LOCUS       DATMTW010000001         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_234882, whole genome shotgun sequence.
ACCESSION   DATMTW010000001 DATMTW010000000
VERSION     DATMTW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234882"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VN084_00005"
     CDS             <1..1367
                     /locus_tag="VN084_00005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWT27865.1"
                     /translation="NQELESRLQANPLLERTDRPEDGGEAESFTPMPNGAETSVAAPA
                     EAPEAETPRQDEFGGGGDDYVEFSGGSNRWDESSAGSDDDNDFTYQEAVSLTLREHLL
                     GQLKLMPLSERDQNLSLLLVDAISDDGYLEQSLEELAELLPEEYEVDPMELETALKHI
                     QHLDPPGIGARSLAECLALQLKAMPEDTPGLQQAIMVAEQHLPLLAARDYVRLRKLLG
                     CDDEALRGVQQLIKQLNPRPGNAFTQIASDHYIQHEIIVKKIKGIWIASLNDEVVPKL
                     RINQLYAGILKRNRDSSSQYLMSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFE
                     HGEVAMRPLVLREIADELGLHESTVSRVTTRKYMLTPRGVYELKYFFGSHVATESGGA
                     CSATAIRALIKQMVNEENPKKPLSDNQITDILGKQGIVVARRTIAKYRESLQIPAANQ
                     RKSL"
     gene            1396..1722
                     /gene="raiA"
                     /locus_tag="VN084_00010"
     CDS             1396..1722
                     /gene="raiA"
                     /locus_tag="VN084_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWT27866.1"
                     /translation="MNLQLTGHHLDVTPALREYVLSKLTRVSNHFDHVIDVKVTLSVE
                     KLIQKVEATLHVPGNDLHATCSDENMYSAIDMLADKLDRQVLKHKEKMSDHHKSDGGV
                     KHQAAE"
     gene            1904..>2205
                     /locus_tag="VN084_00015"
     CDS             1904..>2205
                     /locus_tag="VN084_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HWT27867.1"
                     /translation="MAEISVGQLFKDTRHPLKLKWVAGLNGGDNLLSSERVTKPSLAL
                     IGHLNFVHPNRVQVLGCAEMDYLRSLQEKELQQAIQNLFSTDLAAVVVANGEVAPS"
BASE COUNT          537 a          675 c          639 g          354 t
ORIGIN      
        1 tcaaccaaga gctggaaagt cggctgcagg ccaacccctt gctggaacgc accgatcggc
       61 cggaagacgg tggcgaggct gagagcttca cgcccatgcc caacggcgcg gagaccagcg
      121 tcgccgcccc ggccgaagcc ccggaggcgg aaacgccacg ccaggacgag ttcggcggcg
      181 ggggcgacga ttatgtcgaa ttctccggcg gcagcaaccg ctgggacgag agcagcgccg
      241 gtagcgacga cgacaacgat ttcacctatc aggaagcggt cagcctcacc ctgcgcgaac
      301 acctgctcgg ccagctcaag ctcatgccgc tgtcggaacg tgaccagaac ctgtcgctgc
      361 tattggtcga cgccatcagc gacgatggtt acctggagca atcgctggaa gaactggccg
      421 aattgctgcc ggaagaatac gaagtcgatc cgatggagct ggagacggcg ctcaagcata
      481 tccagcacct ggatccaccc ggtatcggcg cacgcagcct ggccgaatgc ctggcactgc
      541 aactgaaagc catgccggaa gacacgccgg ggctgcaaca ggcgatcatg gtcgccgaac
      601 agcacctgcc gctgctggcg gcacgcgatt acgtgcgact gcgcaaactg ctggggtgtg
      661 atgacgaggc cctgcgcggc gtgcaacagc tcatcaagca gctcaaccct cgcccaggca
      721 acgctttcac ccagatcgcg tcggaccatt acatccagca cgagatcatc gtcaagaaga
      781 tcaaggggat ctggatcgcc agcctcaacg acgaagtggt gcctaagctg cggatcaacc
      841 agttgtatgc cggcatattg aagcgcaacc gcgacagctc gagccagtac ctgatgagcc
      901 agatgcagga ggcgaagtgg atgatcaaga acatccagca acgcttttcc accatcctgc
      961 gcgtatcgca ggccatagtc gaccgccagc gcaacttctt cgagcatggc gaggtggcca
     1021 tgcggccgct ggtgctgcgc gagatcgcag acgagttggg cctgcatgaa tccacggtat
     1081 cacgagtgac gacgcgcaag tacatgctca ctccgcgtgg cgtttatgaa ctgaagtatt
     1141 tcttcggcag ccatgtcgct accgaatccg gaggcgcctg ttccgccacg gcaatccggg
     1201 cattgatcaa acagatggtg aatgaagaga acccgaagaa acccctgtcg gataaccaga
     1261 tcaccgacat cctcggcaaa cagggcatcg tcgtcgcccg acgcaccatc gcgaaatacc
     1321 gcgagtctct gcagataccc gcggcaaacc agcgcaagtc gttgtgacct gcgcgaacca
     1381 ctgaaggaga gcatcatgaa cttgcaacta accggccatc atctcgatgt caccccagct
     1441 ttgcgtgaat atgtcctcag caaactgacg cgcgtcagca atcacttcga tcatgtcatc
     1501 gacgtcaaag tgacgctcag cgtggaaaag ctgatccaga aggtcgaggc gacgctgcat
     1561 gttccgggta acgatctgca cgctacctgt agtgacgaga acatgtatag cgccatcgac
     1621 atgctggccg acaaactgga tcgccaggta ctgaagcaca aggagaaaat gagcgatcat
     1681 cacaagagcg atggtggcgt gaaacaccag gctgccgagt aagcagcatc acctgacgag
     1741 caggacgaga tgcgaagcgc aggcaacatg agaaccgaaa agaccatgcc tcagtgcaag
     1801 ccaggctgca cggcaggtga cgcccgaact gcacatcctt tgcacgggca cgattcgaat
     1861 gcgccggcga ccaagccgcc cgcccttaca acaggtgtca accatggctg agatcagcgt
     1921 agggcaattg ttcaaagaca cccgccaccc gctcaaactc aagtgggtgg caggcctcaa
     1981 cggcggggac aacctgctga gcagcgaacg ggtgaccaag ccctcgctgg cgctgatcgg
     2041 ccatctcaat ttcgtgcatc ccaaccgcgt ccaggtactg ggttgcgccg agatggatta
     2101 cttgcgcagc ctgcaagaaa aagagctgca gcaggcgatc cagaatctct tttccaccga
     2161 ccttgccgct gtcgtcgtcg ccaacggcga agtcgcgccg tcgca
//
LOCUS       DATMTW010000002         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_604020, whole genome shotgun sequence.
ACCESSION   DATMTW010000002 DATMTW010000000
VERSION     DATMTW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604020"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..>2054)
                     /locus_tag="VN084_00020"
     CDS             complement(44..>2054)
                     /locus_tag="VN084_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NEL-type E3 ubiquitin ligase domain-containing
                     protein"
                     /protein_id="HWT27868.1"
                     /translation="VSLLHAVLQCLDDKQKLALGNVGQQVTQLRDKLLEIAAQEREQA
                     ARLIGLAPKGTGVRPPRRFGDGRLAYRLSGGGESSRQAIRRGIHQIFPTLSEMQLEAY
                     MDAVRQRGENLWDHYQMLQRQLAELREVLRQWQADWQNPIDAIRRRRIADTLRRSWRR
                     KLVDGNDQYELSIDGEHVSALPVLPPGVDYGHVRRLTLRNMQLQDIDASFLRLFPNVV
                     DLDLSGNRLSRVPEGIERLSQLRRVNLGNNQIALDEPGSRRLAGLQRLDTLILSYNPL
                     NGVPDLSALPHVRDVRLRATGQVDMSLVHERLALRAHIDLRDNRISELQRDMRGLRLR
                     LRRLNLHENPLNEASVQLLDEARGVSDSRARGGASYSHAAVNAETRAEWVASRNESLR
                     ASREASWDRLQEEPGSAGLFRFLADFAESEDFETHPRHYRRRIWHILDACENNEALRE
                     QLFREADAPRSCEDRLLLILNQMEVAILAYQGIEGVPVAIREARLLRLGRQLHRLDLL
                     DEIAARHVQRMRAEAVVDVDEVETRLYYRSRLASALDLPVTPDEMHYASFAHVSNADL
                     SHAQLEVLAGNTSVAILDSLADRPFWQNYLREAYPERFEALAAPFHERLESLEQQART
                     GQESDYGRRARALMLEHEADERRLMRILTTEAWRRSASIDRPPRA"
BASE COUNT          383 a          657 c          610 g          404 t
ORIGIN      
        1 cgcgtccagg cagcctgacc ctgctggcct cgcctcgtgc cgctcaggcg cgaggcggtc
       61 ggtcaatgga agcagaacgc ctccaggcct cggtagtcaa gatgcgcatc aacctccgct
      121 cgtcagcctc atgctcgagc atcaatgccc gggcacgcct gccgtagtca ctttcctggc
      181 cggttctggc ctgctgttca agcgattcca agcgttcatg gaatggcgca gccagcgctt
      241 cgaagcgctc tgggtaggct tcacgcaggt aattttgcca gaaagggcgg tcagccaaag
      301 aatcgaggat ggccactgac gtgttacccg cgagtacttc cagttgcgca tggctcaggt
      361 cggcgttgct tacatgggca aacgaagcgt aatgcatctc gtctggtgtc actggcagat
      421 caagggcgct tgccaggcgt gagcggtagt acagccgcgt ttcgacttca tcgacgtcga
      481 ccactgcctc cgcgcgcatt ctttgcacat gccgtgcagc aatctcatcc agcaggtcca
      541 gacggtgcag ctgccggccc aggcgcagaa gtcgggcctc ccgtatcgca acggggacac
      601 cttcgatacc ttggtaggca aggatggcca cttccatctg gttaagtatc aacaacaggc
      661 ggtcttcgca gctccggggg gcatcagcct cgcggaacaa ctgctcgcgc aacgcttcgt
      721 tgttttcgca ggcatcgagg atgtgccaga tacggcgacg ataatgacgg ggatgggttt
      781 cgaaatcttc gctttcggcg aaatccgcga ggaaacgaaa caaccctgcc gagccgggct
      841 cctcctgcaa acggtcccag ctagcttcgc gactggcacg cagcgattcg tttcggctgg
      901 ccacccattc ggcacgggtt tcggcgttca ctgccgcgtg ggaataggac gccccgcccc
      961 tggcccggga gtcgctgacg ccgcgcgcct cgtcaagcaa ctggacactg gcttcgttca
     1021 gcgggttctc atgcaaattg agccttcgca gccgcaggcg caggccgcgc atatcccttt
     1081 gcagctcgct gatccggttg tcacgcaggt cgatgtgcgc gcgcaacgca agccgctcgt
     1141 gaaccagact catatcaacc tgcccggttg cacgcagacg cacgtcacgc acatgcggca
     1201 aggcagagag atcaggcaca ccgttgagcg ggttgtagct gagaatcaag gtatcgagcc
     1261 gctgcaaacc cgccagacgg cggctcccag gttcatccag ggctatctgg ttattgccca
     1321 gattgacccg tctcagctgg ctgaggcgct cgatcccctc gggcacgcgg cttagccggt
     1381 tgccgctgag atcaagatcg accacattcg ggaacagcct gagaaacgag gcatcgatgt
     1441 cttgtagttg catgttccgc aacgtcagcc tacgcacatg gccatagtcg acccctgggg
     1501 gcaggacagg cagcgcactg acatgctcac cgtcgatgct cagttcatat tggtcattcc
     1561 cgtcaaccag tttgcgccgc cagctacggc gcaacgtatc ggcgatgcgg cggcgacgga
     1621 tggcgtcgat gggattctgc cagtcggcct gccactgccg cagcacttcc cgcagctcgg
     1681 ccagctggcg ctgcagcatc tggtaatggt cccacaggtt ttcgccacgc tggcgaaccg
     1741 cgtccatata agcttcaagc tgcatttcac tgagcgtcgg gaatatctgg tggatgcctc
     1801 gccggatcgc ctgccggctg ctctccccgc cgccgctcag gcgataagcc aggcggccat
     1861 cgccaaagcg tctgggtggc cgtacgccag tccccttagg ggccaggccg atcagcctgg
     1921 cggcctgttc gcgctcctgc gcagcgattt cgagcagttt gtcccgtagc tgcgtcactt
     1981 gctgcccgac attgcccaag gccagcttct gcttgtcgtc caggcactgc agtacggcat
     2041 gtagcaatga caca
//
LOCUS       DATMTW010000003         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_134324, whole genome shotgun sequence.
ACCESSION   DATMTW010000003 DATMTW010000000
VERSION     DATMTW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134324"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VN084_00025"
     CDS             complement(<1..1105)
                     /locus_tag="VN084_00025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT27869.1"
                     /translation="MAIVDEANHRPALVGPGVELLTYKTPQGAAKTTHPYLQGYEAAI
                     RRGQQIARACLALKQRGFVPDIVCAHAGWGEALFIKEIFPQAILLDYFEFYYRTRGAD
                     VGFDPEIHASSLDDDCRISTRNACHLQSIESADWGWSPTAWQASRFPEHFRAKLSVIH
                     EGIDTKIVKPDADAVFQTPAGLKLTRIEQVVTFVNRNLEPYRGFHIFMRALPRILRDH
                     PHAQVVIVGGDRVSYGSPPREGGTWRECMLAEVGSELDMQRVHFVGKLPYPAYLQLLQ
                     VSSAHVYLTYPFVLSWSMLEAMAAGCALIASDTPPVTEVVRHTHDALLVDFFDIEALA
                     EQVGRVLNKPQEYQAMREQARKTIHERYDLESVC"
     gene            complement(1211..>2279)
                     /locus_tag="VN084_00030"
     CDS             complement(1211..>2279)
                     /locus_tag="VN084_00030"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HWT27870.1"
                     /translation="MASLFDFNAISALFASGFRMKFDGMHAVTGPYAHEIFVNQLGAS
                     QEMLMNCVPLPDFGGGHPDPNLTYAHDLVDILYGSDAPDFGAASDGDGDRNMILGKGF
                     FVTPSDSLAILAASARLVPGYVDGIAGIARSMPTSAAADRVAEELGVPCYETPTGWKF
                     FGNLMDAGKVTLCGEESFGTGSSHVREKDGLWAVLFWLNILAIKRQSVEDLVKRHWAR
                     FGRNVYSRHDFEGLPSEAANAIMQHLREHFAVWPDQAFGRYTVKQCDDFSYTDPVDGS
                     VSKAQGVRIQFTDGSRIVFRLSGTGTEGATLRIYLEAYEPDVSQHHLDAQEALAEMIQ
                     IALQLSQLVERTGRKKPTVIT"
BASE COUNT          486 a          699 c          661 g          433 t
ORIGIN      
        1 aacataccga ttcaagatcg taacgctcgt ggatggtctt ccgcgcttgt tcacgcatgg
       61 cctgatattc ctgcggctta ttcaataccc tacccacctg ttcggcgagc gcctcgatat
      121 cgaagaaatc gaccagcagg gcgtcgtgag tgtgtcgcac cacttccgtc accggcggag
      181 tatcggatgc gatcagcgcg caacccgcag ccatcgcctc cagcatcgac caggacaaga
      241 cgaatggata ggtcagatag acatgcgcgg acgacacctg aagcaactgc agataagccg
      301 gataaggcag cttgccgacg aaatgcacgc gctgcatgtc gagttcgcta ccgacttcgg
      361 cgagcatgca ctcacgccag gttccgcctt ctcgcggcgg actgccgtag ctgacccgat
      421 cgccgccgac gatcaccacc tgcgcatgcg gatgatcgcg caggatccgc ggcaaagccc
      481 gcatgaagat atggaaaccg cgatacggtt cgaggttacg gttgacgaag gtcaccactt
      541 gttcgatccg ggtcagcttc agtccggccg gcgtttggaa caccgcatcc gcgtccggct
      601 tgacgatctt ggtatcgata ccctcatgga tgacactgag cttggccctg aagtgctccg
      661 ggaagcggct tgcctgccac gctgtcggtg accagcccca gtctgcggat tcgatcgact
      721 gcagatgaca ggcgttacgc gtgctgatgc gacaatcgtc gtccagcgat gaggcgtgga
      781 tctccggatc gaacccgaca tccgcgccac gggtccggta gtagaactca aaataatcga
      841 gcaggatcgc ttgcgggaag atctccttga tgaacagcgc ttcgccccaa ccagcatggg
      901 cacaaacgat gtcaggcacg aagccgcgct gcttcaacgc gaggcacgca cgcgcgatct
      961 gctgcccacg tcggatggca gcctcgtagc cttgcaggta tggatgcgtg gtcttcgccg
     1021 ccccttgcgg cgtcttgtaa gtcaacagct ccacgccggg ccccaccaac gcggggcggt
     1081 ggttggcttc atcgacgatg gccacgaccc gattgcccgc gtcaaccgcg agcgccgagg
     1141 cgatatgcct gaattgccca ggaaaattct gatggatgaa aaggtatcgc acaggcggtc
     1201 agctggccga tcaggtgatc accgtgggtt tcttgcggcc agtacgttcg accagttgcg
     1261 acagttgcaa cgcgatctgg atcatctcgg ccaatgcctc ttgcgcatcc agatgatgct
     1321 gggatacatc cggctcatag gcttcgagat agatacgcaa ggtggcgcct tccgttccgg
     1381 tgccggaaag gcggaagacg atacgtgaac catcggtgaa ctggatgcgc acaccctgcg
     1441 ccttgctcac gctgccgtcg acgggatcgg tatagctaaa atcgtcgcat tgcttgaccg
     1501 tataacggcc aaacgcctga tctggccaga ccgcgaaatg ctctcgcaga tgctgcatga
     1561 tggcgttggc agcttcgctc ggcaggcctt cgaaatcatg tcgcgaatag acattgcggc
     1621 cgaatcgcgc ccaatggcgc ttgacgaggt cttcgaccga ctgacgcttg atcgccagga
     1681 tgttgagcca gaacagtacc gcccacaagc cgtccttctc gcgcacatgg ctagagccag
     1741 tgccgaagct ctcctccccg cacaaggtga ccttgcctgc atccatcaga ttgccaaaga
     1801 atttccagcc ggtcggggtc tcgtagcatg gcacgccaag ctcttccgcc acgcgatcgg
     1861 cggcggcgct ggtcggcatg gaacgcgcaa tgccggcgat gccgtcaacg taacccggta
     1921 ccaggcgtgc gctcgccgcc aatatcgcca aactgtccga tggtgtgacg aaaaacccct
     1981 tgcccaggat catgttccgg tcgccatcgc cgtcagaagc agcgccaaaa tccggggcgt
     2041 cggagccata caagatgtcc accagatcat gggcataggt cagattgggg tccggatggc
     2101 cgccaccgaa atccggcaag ggtacacaat tcatcagcat ttcctggctt gcgccaagct
     2161 ggttgacgaa tatctcgtgc gcatacgggc cggtcacggc gtgcatgccg tcgaacttca
     2221 tgcggaaccc gctggcgaac aatgcgctga tcgcattgaa atcgaacaac gaggccatg
//
LOCUS       DATMTW010000004         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_162, whole genome shotgun sequence.
ACCESSION   DATMTW010000004 DATMTW010000000
VERSION     DATMTW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VN084_00035"
     CDS             <1..1014
                     /locus_tag="VN084_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWT27871.1"
                     /translation="GGAHLKAALVDDEGHALQVIQVACQLWLGLHELNLAVAKVQDKL
                     SHVPAQHAITMTGELADIFPDRESGVRQIAEVMNDVLHCKPRFFAGKHGLIAATELPV
                     YLKDIASANWLASATFVAGKLPQGLFVDIGSTTADLSLVRDGAVLNRGFSDAERMQCE
                     ELVYTGVVRTPLMALAKRIPFAGEWQVVAAEHFATTADVYRLTAELNPAEDMAETADG
                     AAKTLHDSARRMARMIGRDLADAPHAAWEELAYAFRQAQLDLLKSAVLRQLSRHQLAS
                     DAPLIGAGAGSFLVATLAAQLGRPFIDIGSLIRGYNAHSQRWASVCLPAYAVACLLND
                     NAG"
     gene            1016..2404
                     /gene="pabB"
                     /locus_tag="VN084_00040"
     CDS             1016..2404
                     /gene="pabB"
                     /locus_tag="VN084_00040"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HWT27872.1"
                     /translation="MREYELPYEEDGARLFARVANRPWAIYLDSGQPESQYGHYDIIV
                     CDPFMTLSTVAGVTIIEDRAGRRQSTADPFTLLKEALAEYPAEACALPFCGGALGYFA
                     YDLGRRLERLPALAEDAEHMPEMRVGLYDWAVIVDHKAKVARLVSHVRHPTTEAAWDA
                     LCALFSAPPPEQAEAFAVTSRVGSNMDEAQYRQAFDRIQRYIAEGDCYQVNLAQRFVA
                     KAQGDAWTAYRQLRRISPAPFMAYMNFGDLQVLSASPERFLQVVGEHVETRPIKGTRP
                     RASDSAADAAHAQALQSSLKDRAENLMIVDLLRNDISKSCRVGTVRADRLFALESFAN
                     VHHLVSTVTGTLKPGMTAVDLLRGCFPGGSITGAPKLRAMEIIEELEPNRRGLYCGAI
                     GYIGFDGNMDTNIAIRTAVYSNGEFRFWAGGGIVADSEMDKEYRETWDKASSMLVLMQ
                     YFGGEVTHDLGR"
     gene            2388..3017
                     /locus_tag="VN084_00045"
     CDS             2388..3017
                     /locus_tag="VN084_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HWT27873.1"
                     /translation="MTWVVKLGGSMLDSSELKHWLDMFARHSDGQIVIVPGGGLFADA
                     VREAQYMTGIDDETAHRMAVVAMDQYGMLMTGLNHDLVTASTELEIAERGWQHRAIVW
                     LPSHMVCGEESIAASWHVTSDSLAAWLAARINATQLVLVKSARPDIEQISLERLVKDG
                     FVDASFGDYIAGQPFETWLLGKQDHAAFNEKLTDQRLQEIGLQVRCAWN"
     gene            3029..3412
                     /locus_tag="VN084_00050"
     CDS             3029..3412
                     /locus_tag="VN084_00050"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWT27874.1"
                     /translation="MSETNHEKTYSEAEVTARLHQELPHWYYEDGWIRRKYKTGSWKG
                     TLMVVNTVGHLAEAAWHHPDLTVSYAFVVVKLCTHSAKGVTEKDFALAKKIEEVIQWQ
                     PGREAGAALEGTPNEDQRFKYIKYD"
     gene            3461..4597
                     /locus_tag="VN084_00055"
     CDS             3461..4597
                     /locus_tag="VN084_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWT27875.1"
                     /translation="MSGHPVVSWQEAGGISSARWRSENGAPPPTRVVIGDDTMTADAA
                     YHLACEGTAILWRGDYQNARQLLQAMARRIDRKPRRSRSANIGKTTAEAFHLHRQAQS
                     QRARILGMLLLPIATEHRLGLRRAPDVAAACEAAYGAATEPYVASLRELLGLIGAYEW
                     RKKGVEIQPLGAKIHPFYGVFSPVRGEYLALVAEAPLPASTLAFDIGTGTGVLAAILA
                     KRGIRHVIATDQDARALACARENIAALGYASQVEVVEADLFPQGKAPLIVCNPPWIPA
                     RPSAAIEYAIYDPESRMLKGFLNGLTEHLAPDGEGWLILSDIAEHLGLRTRETLLAMF
                     EAAGLQVVSRMDTRPVHGKATDTADPLHAARAAEITSLWRLRLA"
     gene            complement(4626..>4808)
                     /locus_tag="VN084_00060"
     CDS             complement(4626..>4808)
                     /locus_tag="VN084_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWT27876.1"
                     /translation="LVTALGLLPLAAGSGQPGREIEGPMATIIVGGLVTSTILNLLIL
                     PTIMLHFGRFEKRLQD"
BASE COUNT          924 a         1491 c         1509 g          884 t
ORIGIN      
        1 gggggcgctc accttaaggc cgccctagtc gatgacgaag gccacgcgct gcaggtgatc
       61 caggtcgcct gtcagctctg gctgggcctg catgagctca atctcgcagt ggcaaaggtg
      121 caggacaagc tcagccatgt accggcgcag catgcgatca ccatgaccgg ggagctggcc
      181 gatatctttc cggatcgtga aagcggcgtg cgtcagatcg ccgaagtcat gaacgatgtc
      241 ctgcattgca agccccgttt ctttgccggc aaacacgggt tgatcgccgc aaccgagctt
      301 cccgtatacc tgaaggacat tgcctccgcc aactggctcg ccagcgctac cttcgtcgcc
      361 ggcaaattgc cgcagggtct gttcgtcgac atcggcagca ccacggctga cctttcactc
      421 gtgcgcgatg gggctgttct gaaccgcggt ttttccgatg ccgagcgcat gcagtgcgaa
      481 gagctggtct ataccggcgt ggtgcgcaca ccgctgatgg cgctggccaa acgcatcccc
      541 ttcgctggcg agtggcaggt ggtggctgcc gagcatttcg ccaccacggc ggatgtctac
      601 cgcttgacgg cagaactgaa ccctgcagaa gacatggccg agactgccga cggtgcggcc
      661 aagaccctgc atgacagcgc caggcgcatg gcacggatga tcggtcgtga tctcgccgac
      721 gcgccgcacg cggcctggga agaattggcc tatgcgttcc gccaggcgca actcgacctg
      781 ctcaagtccg cggtcttgcg gcagctgtct cgccaccagt tggcgtcgga tgcaccgctc
      841 atcggcgccg gcgccggcag tttcctggtg gcgacgctgg ccgcccagct tgggcgaccg
      901 ttcatcgata tcggcagcct cattcgcggc tacaacgccc atagccagcg ctgggcatca
      961 gtctgcctgc cggcctacgc ggtcgcctgc ctgcttaacg ataacgcggg ctgacatgcg
     1021 cgaatacgag cttccttatg aagaggacgg agcgcggtta ttcgcccgcg tcgccaaccg
     1081 cccctgggcg atctatctcg acagcggcca gcccgaaagc cagtacggac attacgacat
     1141 catcgtctgt gaccctttca tgaccttgtc cacagtggcc ggcgtgacga tcatcgaaga
     1201 ccgcgccggt cgccgccaat cgacggccga tccgttcacg ctgctgaagg aggccttggc
     1261 cgaatatcct gcagaggcct gcgccttgcc attctgcggc ggtgcccttg gctatttcgc
     1321 atatgatctt ggccgccgac ttgagcgctt gccggcgctg gccgaagacg cggagcacat
     1381 gcctgagatg cgcgtcgggc tctatgactg ggccgtgatc gtcgatcaca aggcgaaggt
     1441 cgcgcgtctt gtctcgcatg tcagacaccc caccacagag gcggcatggg atgcgctttg
     1501 cgccttgttt tcggcgccac ccccagaaca ggcggaggct tttgccgtca cctcccgtgt
     1561 cggctcgaac atggacgagg cgcaatatcg acaagccttc gatcgcatcc agcgctacat
     1621 tgctgaaggc gactgctatc aggtcaacct ggcacaacgt tttgtcgcca aggcgcaagg
     1681 cgacgcgtgg acggcctatc gccagttgcg ccgcatcagc cccgcgccct tcatggccta
     1741 catgaatttc ggcgacctgc aggtcctgtc ggcctcgcca gagcgtttcc tgcaggttgt
     1801 gggagagcat gtcgagacac ggccgatcaa gggcacacgt ccccgcgcca gcgattcggc
     1861 agccgatgcc gcgcatgcgc aggcgttgca gtccagcctc aaggatcgcg ccgagaacct
     1921 catgatcgtc gatctgttac gcaacgacat cagcaaaagc tgtcgtgtcg gcacagtgcg
     1981 tgcggatcgc ctgttcgcac tcgagagttt cgccaacgtg catcatctgg tgagcaccgt
     2041 caccggtacc ctgaagccgg gcatgacggc agtggacctg ctgcgtggct gcttccccgg
     2101 cggttcgatc accggtgcgc ccaagttgcg cgcgatggag atcatcgagg aactggagcc
     2161 gaaccgtcgc ggcctctatt gcggagcgat cggctacatc ggtttcgatg gcaacatgga
     2221 caccaatatc gccatccgca cagcggtcta ttcgaatggc gagtttcgtt tctgggccgg
     2281 cggcggcatc gtcgccgatt ccgagatgga caaggagtat cgtgaaacct gggacaaggc
     2341 atcgtcgatg ctggtcctga tgcagtattt tggaggtgag gtgacgcatg acctgggtcg
     2401 ttaagcttgg cggcagcatg ctggattcat cagaactcaa gcactggctg gatatgttcg
     2461 cgcgtcacag cgatggtcag atcgtcatcg tgccgggcgg cggcctgttc gccgacgccg
     2521 tgcgggaagc gcagtacatg accgggatcg atgacgagac ggcacaccgc atggcggtag
     2581 tcgcgatgga ccagtacggc atgctgatga cgggtctcaa tcatgatctg gtgacggcct
     2641 ccaccgagct ggagatcgcc gaacgcggct ggcaacaccg tgccatcgtc tggttgccga
     2701 gccatatggt ctgcggcgaa gagagcatcg ccgccagctg gcacgtcacc tcggatagcc
     2761 tcgctgcatg gctcgcggca cgcatcaacg ccacgcaact ggtgctggtc aaatccgcac
     2821 gccccgacat cgagcagata tcgctcgaac ggttggtcaa ggacgggttc gtcgacgcca
     2881 gcttcggtga ctacattgcc ggccagcctt tcgagacctg gctgcttggc aagcaggacc
     2941 acgccgcatt caacgagaaa ctgaccgacc agcggctgca ggagatcggc ctgcaagtac
     3001 gttgcgcctg gaattgaagg agaacgagat gagcgaaacc aaccacgaga agacctatag
     3061 cgaagccgag gttacggctc gcctgcatca ggaactgccg cactggtact acgaagatgg
     3121 ctggatccgc cgcaaataca agaccggtag ctggaagggc accttgatgg tggtcaatac
     3181 cgttggccat cttgccgagg cggcctggca tcatccggac ctgaccgtgt cctatgcttt
     3241 tgtggtcgtc aaactctgca cgcacagcgc caagggcgtc accgagaagg acttcgcgct
     3301 ggcgaagaag atcgaggaag tgatccagtg gcagccgggc agggaggcag gcgccgcgct
     3361 cgaaggcacg ccgaacgagg atcagcgttt caagtacatc aaatatgatt gatgcgcggc
     3421 ggagaccacc tggcggacgg tcctcgaatg gcggctacag atgagcggcc atccggtcgt
     3481 aagctggcaa gaggcgggtg gcatatccag cgcgcgatgg cgctcagaga atggcgcacc
     3541 accgccgaca cgagtcgtca tcggcgacga caccatgact gccgatgcgg cttaccatct
     3601 tgcctgcgaa ggcactgcca tcctctggcg cggcgactac cagaacgccc gccagctgtt
     3661 gcaggccatg gcgcgacgta tcgatcgcaa gccgcgcaga tcccgctcgg ccaacatcgg
     3721 caaaaccact gcagaggcgt tccatctgca tcgccaggcg caatcccagc gtgcgcgcat
     3781 cctcggcatg ttgttattgc cgatcgccac cgaacatcga cttggattgc gtcgcgcacc
     3841 tgatgtcgct gccgcttgcg aagccgcata cggcgcggcc actgagcctt atgtcgcgtc
     3901 cttgcgggaa ctgctggggc tgatcggcgc ttacgagtgg cgcaagaagg gggtcgagat
     3961 acagcctctg ggggcaaaga tccatccgtt ttacggggtc ttttcccccg tgcgcggcga
     4021 atacctggcg ctggtcgctg aagcgccgtt gccagcatca acgctggcgt tcgatatcgg
     4081 caccggcact ggtgtgctgg cggcgatcct ggccaagcgc ggcatacgac atgtgattgc
     4141 gaccgaccag gatgcacgtg cattggcctg tgcccgcgag aatatcgcgg cgctggggta
     4201 cgcaagccag gtcgaagtcg tcgaagcgga tttgtttccg caaggcaagg cgccgctgat
     4261 cgtctgcaac ccgccctgga tcccggcacg cccaagcgca gccatcgaat atgccatcta
     4321 cgacccggag agccgcatgc tcaaggggtt cctcaatggc ctgacggaac accttgcgcc
     4381 ggacggggaa ggttggctga tcctctcgga catcgccgag catctggggt tgcgtacaag
     4441 ggaaaccttg ctggccatgt tcgaggctgc tggcctgcaa gtggtctcac gcatggatac
     4501 gcgcccggtg cacggcaagg cgacggatac agccgatccg ctgcacgcgg cgcgcgccgc
     4561 cgagatcaca tcgctctggc ggctacgctt ggcctaggtt cggtccggtc tggatccggt
     4621 gtgactcagt cctgcaagcg tttttcaaag cgcccgaaat gcagcatgat ggtaggcagg
     4681 atcaacaagt tcaggatcgt ggaagtcacc agcccaccga cgatgatggt tgccatcggg
     4741 ccttcgatct cacggcccgg ctgaccactg ccggccgcga gcggcagtag gccaagcgcg
     4801 gtgaccag
//
LOCUS       DATMTW010000005         6949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_436333, whole genome shotgun sequence.
ACCESSION   DATMTW010000005 DATMTW010000000
VERSION     DATMTW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436333"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1186
                     /locus_tag="VN084_00065"
     CDS             359..1186
                     /locus_tag="VN084_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HWT27877.1"
                     /translation="MLFALACQAEETILDLRTGDTLTRAQLVEQIAQQDVVLLGEIHD
                     DVRHHALRGQLLRDVAARRPLQVVAEQLESGQVFHRQGTLLQGLQLSGFDPMLWAWPA
                     HEALFAAIDQAGIPLVGGNIPLPVAKSISRQGEAAMPADLLATIEQAPLSVPASTTLD
                     DDLLAGHCGFLKPAMLPTMRLTQRARDAAMFDALQARPAGTLGVLVAGNGHVRMDYGV
                     PVLLTRHSPQSRWLSIGFIEQQDQLDALLPTLKQRYTHVWITPGHARGDPCVAFGKP"
     gene            1307..2239
                     /locus_tag="VN084_00070"
     CDS             1307..2239
                     /locus_tag="VN084_00070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT27878.1"
                     /translation="MRTTDNTMQDDSTVYKTLLESTKAIPWKIDWATMQFAYIGPQIE
                     QLLGWTPDSWQTANDWAERMHPEDREWVVNFCVSQSQSGIDHEADYRALTKDGHYVWI
                     RDVVHVVRKENGEVDSLIGFMFDISERKKTEEQLANLRKELEEMSYKDGLTGVANRRM
                     FDSVLELEWSNARRSRQPLSLIMLDIDHFKEYNDHYGHIQGDDCLIRVAKVLATAATR
                     SRDFIARFGGEEFVLVLPETDAEAAAKIAERCRQLIFKAQIPHLNSSVSNVITISVGV
                     GTILPSHQDQPMAFVEATDKKLYLAKQQGRNRVA"
     gene            2466..4943
                     /locus_tag="VN084_00075"
     CDS             2466..4943
                     /locus_tag="VN084_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWT27879.1"
                     /translation="MEISEGRYRTLFEYAPDGILIANTQGYYLDANPRMCEMLGYSHD
                     ELTHLHSTDIVAPSEQQYVEPVLDEVLSNNRHQRLWIFQRKDGSQFEAEVFVTAMPDG
                     NLLGMVRDVSEARAHEREIARLSSLYEALSRINHTVVLRSTRTELLQSICQILVEQGG
                     LCMAWASRYNPTTRQLEPMAIAGDDVGYIQSIKIYADERPEGQGPSGICFRTGAPFIC
                     NDMQNNPVTLPWREEIVRRRFKASAAFPIRQFGEVWGVLNVYSDRTEFFHEKEIALLQ
                     EASTDVSFALDNIAHEEARKSAESEARNERRFSETMVDSMPGILYFYDDQGRFLRWNK
                     NFEAVSGYSADEITRMHPLDFFPEHEKTMLQTRIGEVFVQGESSVQATFLCKDGTLRP
                     HFFTGRKVRFNGMDCLVGVGIDITDRIEAESRLAESEQKYRELVELANSIILRWDAEG
                     RITFLNEYGQKFFGYTLDEIIGRHVIDTLVPDTDSEGRDLKSLMAHICANPKAFEQNI
                     NENIKRNGERVWVAWTNKFVSDADGNIQEILSIGSDITEQREAELAVQELNATLEQRV
                     IDRTEALNAALVRAEAADKIKSAFLATMSHEFRTPLNSIIGFTGIILQGLAGPLNEEQ
                     TKQLSMVRGSARHLLELINDVLDISKIEAGQLEVHPQPFDLKESIERVVASVRPLAEK
                     KGLTLSMTMAGVPDSMESDRRRLEQILLNLLNNAIKFTETGSVSLNIAPAPVIAEDGV
                     KRPGVRFSVKDSGMGIKPEDLGQLFQAFHQIDSGLTRQYEGTGLGLAICRRLANLLGG
                     EVSATSVWGAGSEFSVILPLHAEAVTT"
     gene            4940..5332
                     /locus_tag="VN084_00080"
     CDS             4940..5332
                     /locus_tag="VN084_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWT27880.1"
                     /translation="MSTTILMIEDNEQNRYLATFLLKQHGYEIVSAFDGPSGISMAQT
                     LKPALILLDIQLPGMDGYSVAQALRSIGALEATPIVAVTSYAMVGDREKALAAGCNGY
                     IEKPINPDTFVSEIGKFLPSQTAVQGQP"
     gene            5329..>6949
                     /locus_tag="VN084_00085"
     CDS             5329..>6949
                     /locus_tag="VN084_00085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT27881.1"
                     /translation="MTRILIVDDKDENLYYLQALLQGYGYEVDSAKHGAEALVRARQS
                     APDVVISDLLMPVMDGYTLLRHWKIDYALKRIPFIVYTATYTEPEDERLALSLGADAF
                     ILKPAEPEDFIEKLRAVEKRVAKGVTAQPSAGTEEHMLKVYSETLIRKLEEKTLQLEE
                     ANRELQLDIARRKEAEDKIEHLAFYDPLTGLPNRRLMQDRLQHSFAAASRHQLQGALL
                     FIDLDHFKNLNDTKGHNFGDSLLHMVAERLQSCVREGDTVARLGGDEFVVILENLSEN
                     AEQAAALAEAVGDKVLATLGQPYLIGGDEYHCTASIGVSLFKKHEATVEELVRRADTA
                     MYEAKNSGRNTLRFYDPAMQAALEARMQLESDLRHALTADQFKLFYQPQVNTQGEVFG
                     AEVLIRWISPEKGLIPPMQFIPLAEETGLIVPIGNWVLQAACDQLLQWRKDPRTARLQ
                     LAVNVSARQFHQPDFIEQVLQILIDKKVDPSRLKLELTESLVLDDINSAITKMNALKE
                     AGISFSMDDFGTGYSSLTYLAQLPLTHLKIDQ"
BASE COUNT         1490 a         2168 c         2033 g         1258 t
ORIGIN      
        1 taggctgcgg tggcggacgc aatccggccc atccttatcc acgcagtaag gcttgaattt
       61 gcaaaagcag gtgagcgcga cgacatcatc cagcgtgggt gcatccgagc gctcacgcag
      121 caactgtgcg aaggcctgcc tttcctgttc cattcgatct gtctgatcca tcggtctcac
      181 tctccccaaa acaagtcttt gagactatca agccggccgc ttgttcactg ccgaagcaaa
      241 attcagctac acaggcaatt cggagcatcg gcattgaaca aatcgggcgt cagagcgtct
      301 ttgtgaagta cctcgttaga attggatatc catgcgactg ctgctcacat gcctgactat
      361 gttgttcgcc ctcgcctgcc aggcggagga gactattctc gacctgcgca cgggagacac
      421 gctgacgcgc gcgcaactcg tcgagcagat cgcgcagcag gatgtcgttt tgctcggcga
      481 gatccacgat gacgttcgcc atcacgcgtt gcgcgggcag ttgctgcgtg atgttgccgc
      541 cagacggccg ttacaggtag tggccgagca gctcgagagt ggccaggtat tccacaggca
      601 gggcacgctc ttgcaaggcc tgcagctgtc ggggtttgat ccaatgcttt gggcctggcc
      661 tgcgcatgag gccctgttcg ccgccatcga ccaagccggg atcccgctgg tgggcggtaa
      721 cattccgttg ccggtggcga agagcatctc gcgccagggc gaagcggcca tgccggccga
      781 cctgctggcg acgatcgaac aggcgccatt gtcggtgcct gccagcacaa cactcgatga
      841 cgatctactc gccggccatt gcggcttcct taaaccggcc atgctgccga cgatgcgcct
      901 gacccagcgc gcgcgcgacg ccgcgatgtt cgacgccttg caggcaaggc cggccggtac
      961 gctgggcgtg ctcgtcgcgg gcaacggcca tgtgcgcatg gattacggtg tgcctgtgct
     1021 gctgacgcga cattctccac aaagtcgctg gctgagcatt ggcttcatcg aacagcaaga
     1081 ccagctggac gcgctgctgc cgaccctcaa gcaacgctac acccatgtct ggatcactcc
     1141 tggccacgcg cgtggcgacc cctgcgtagc attcggcaaa ccctagccgg cagccggcca
     1201 acacatggcc agcgcgtagt gccggcctgc actggtttca cctgcatcac ttcgttcaca
     1261 tttgataggc gatgcgatac agtgaatacc tctcgcagga ggccatatgc gcaccaccga
     1321 caacaccatg caggacgaca gtaccgtcta caagaccttg ctcgaatcga ccaaggccat
     1381 cccctggaag atcgactggg cgaccatgca gttcgcctat atcggtccgc agatcgagca
     1441 attgctgggt tggacgcccg acagctggca gacggccaac gactgggccg agcgcatgca
     1501 cccggaagac cgtgaatggg tcgtcaactt ctgcgtttcg caatcccaga gcgggataga
     1561 ccatgaggca gattaccgcg cgctgaccaa ggacggccat tacgtctgga tccgcgatgt
     1621 ggtgcatgtg gtacgcaagg agaacggcga agtcgattcc ctgatcggtt tcatgttcga
     1681 tatcagcgag cgcaagaaga ccgaggaaca gttggccaac ctgcgcaagg aactggaaga
     1741 gatgtcttac aaggacggtc tcaccggtgt cgccaaccgc cgcatgttcg attcggtgct
     1801 ggagcttgaa tggtcgaacg cgcgtcgcag ccggcagccc ttgtcgctga tcatgctcga
     1861 tatcgaccat ttcaaggaat acaacgacca ctatggccac atccagggcg acgattgcct
     1921 gatccgcgtg gccaaggtat tggccacagc cgccacgcgc tcgcgtgatt tcatcgcccg
     1981 tttcggcggt gaggagttcg tgctggtgct gccggaaacc gacgccgaag ccgccgccaa
     2041 gatcgccgag cgttgccgcc agcttatctt caaggcgcag atcccccacc tcaattcctc
     2101 tgtcagcaac gtcatcacca tcagcgtcgg tgtcggtacc atcctgccca gtcatcagga
     2161 tcagccgatg gcgttcgtcg aagccaccga caaaaagttg tatctggcca aacagcaggg
     2221 tcgcaaccgg gtcgcctgat cctgcacaac atgcactcga tgcagccagg gctgtggcgg
     2281 cctctggggc gtcttgctgt gcttgtaact tcccgcgcca aattctatgc agcagattgg
     2341 ccgacagatg gatagaccga tcgtctatct agctgatagg attgtcaaaa caacaaaaaa
     2401 gccttacggc gaaagttgtg catgtgaaca agcgatcggg gaatcagagt catcacgctc
     2461 tgcctttgga aatcagcgaa ggccgctatc gcacactctt cgaatacgcg cctgacggca
     2521 tcctcatcgc caatacccag ggctactatc tcgacgccaa cccgcgcatg tgcgagatgc
     2581 tgggttacag ccacgatgaa ctgacgcacc tgcattccac cgatatcgtc gcgccctccg
     2641 aacagcaata tgtcgaaccg gtgctcgacg aagtgctcag caacaatcgc caccagcgtc
     2701 tatggatctt tcagcgcaag gacggcagcc agttcgaggc tgaagtgttc gtcactgcaa
     2761 tgccggacgg caacctgctc ggcatggtgc gtgatgtcag cgaagcccgc gcccatgaac
     2821 gcgagatcgc ccgcctgtcg agtctctatg aagccctgag ccgcatcaac cataccgtgg
     2881 tgttgcgctc gacgcgtacc gagttgctgc agagcatctg ccagatcctg gtcgaacagg
     2941 gcggcttgtg catggcctgg gccagccgtt ataacccgac gacccgccag ctggagccga
     3001 tggcgatcgc cggcgacgat gtcggctata tccagtcgat caagatctat gccgatgaac
     3061 gccccgaagg gcaggggcct tcgggtatct gtttccgtac cggcgcaccc ttcatctgca
     3121 acgacatgca gaacaacccg gtgacgctgc cctggcgtga ggagatcgtg cgtcgccgct
     3181 tcaaagcctc ggccgccttt cccatccgcc agttcggcga agtctggggc gtgctcaacg
     3241 tatactccga tcgcaccgaa ttcttccatg agaaagagat cgccttgctg caggaggcga
     3301 gcaccgatgt ctcgttcgcg ctcgataaca tcgcccatga agaggcgcgc aagagcgcag
     3361 aatcggaagc gcgcaacgag cggcgtttct ccgaaaccat ggtcgacagc atgccgggca
     3421 tcctgtattt ctatgacgat caggggcggt tcctgcgctg gaacaagaac ttcgaggcgg
     3481 tctccggata ttcggccgac gagatcacgc gcatgcatcc gctcgatttc ttcccagagc
     3541 acgaaaagac catgctgcag acacgtatcg gcgaagtctt cgtgcagggc gaatcgtcgg
     3601 tccaggcgac attcctgtgc aaggacggga cactgcggcc gcatttcttc accgggcgta
     3661 aggtgcgctt caacggcatg gattgcctgg tgggcgtcgg catcgacatc accgaccgta
     3721 tcgaggccga gtcgcggttg gcggagagtg agcagaaata ccgcgagctg gtcgagctcg
     3781 ccaacagcat catcctgcgc tgggatgccg agggccgcat cactttcctc aacgagtacg
     3841 gccagaagtt cttcggatac acgctggacg agatcatcgg ccgccatgtg atcgatacac
     3901 tcgtgcccga taccgatagc gagggccgcg acctcaaatc gctcatggcc catatctgcg
     3961 ccaaccccaa ggccttcgag cagaacatca acgagaacat caaacgtaac ggcgaacggg
     4021 tgtgggtggc ctggaccaac aagttcgtca gcgacgcgga cggcaatatc caggagatcc
     4081 tgagcatcgg cagcgacatc accgaacagc gcgaagccga gctggcggtg caggaactca
     4141 acgccaccct ggaacagcgc gtgatcgatc gcaccgaggc actgaacgcg gcgctggtgc
     4201 gcgctgaggc cgcagacaag atcaagtcgg ccttcctcgc caccatgtct cacgagttcc
     4261 gcacgcccct gaattccatc atcggcttca ccggcatcat cctgcagggc ctggcggggc
     4321 cgctcaacga agaacagacc aaacagctca gcatggtgcg cggcagtgcc cgtcacctgc
     4381 tcgagctgat caacgacgtg ctcgatatct ccaagatcga ggccgggcaa ctggaagtgc
     4441 atccacaacc gttcgatctg aaggaatcca tcgaacgcgt ggtcgcctcc gtgcggccgc
     4501 tggccgagaa gaaagggctg acgctgtcca tgaccatggc cggcgtgccc gacagcatgg
     4561 agagcgatcg tcgtcggctg gaacagatcc tgctcaacct gctcaataac gccatcaagt
     4621 tcaccgagac cggcagcgtc agcctgaaca ttgcgccggc gccggtgatc gctgaggatg
     4681 gcgtcaaaag acccggcgtg cgtttcagcg tgaaggacag cggcatgggc atcaaaccgg
     4741 aagacctggg ccagttgttc caggccttcc atcagatcga ctccggcctc acgcggcaat
     4801 acgaaggcac gggcctcggt cttgcgatct gccggcgcct cgccaacctg ctcggtggcg
     4861 aagtcagcgc tacaagcgtt tggggcgcgg gcagcgaatt cagcgtcatc cttccactgc
     4921 atgcggaggc tgtaacgaca tgagcaccac catcctgatg atcgaagata acgagcagaa
     4981 ccgctatctg gccaccttcc tgctcaagca gcacggctac gagatcgtgt ccgcctttga
     5041 cggcccgagc ggcatcagca tggcgcagac attgaagccg gccttgatcc tgctggatat
     5101 ccagctgccg ggcatggatg gttattcggt cgcacaagcg ctgcgcagta tcggggcgct
     5161 ggaggcaacg cccatcgtcg ccgtcacctc gtacgccatg gtcggcgacc gcgaaaaggc
     5221 gctggcggcc ggctgcaacg gctatatcga gaagccgatc aaccctgaca ccttcgtctc
     5281 ggagatcggc aaattcctcc catcgcaaac cgcagtgcag ggccagccat gacacgcata
     5341 ctgatcgtcg acgacaagga tgagaacctc tattacctgc aggccctgct ccagggctac
     5401 ggctacgagg tggatagcgc caagcatggc gccgaagccc tggtgagggc gcggcaatcg
     5461 gcacctgacg tcgtcatctc cgatctgttg atgccggtga tggacggcta tacgctgctg
     5521 cggcactgga agatcgacta cgcgctgaag cgcatccctt tcatcgtgta taccgccacc
     5581 tacacagagc cggaggacga acggctggcg ctgagcctcg gcgccgatgc cttcatcctc
     5641 aagccggccg agcctgagga tttcatcgaa aagctacgcg cggtggagaa gcgcgtcgcc
     5701 aagggcgtga ccgcacaacc gtcggcaggt accgaagaac acatgctgaa ggtctacagc
     5761 gagacactga tccgcaagct cgaggaaaag acgctgcagc tggaagaagc caaccgcgag
     5821 ctgcagctcg atatcgcacg gcgcaaggag gccgaagaca agatcgagca tctcgccttc
     5881 tacgatccgc tcaccggcct gcccaaccgc cgcctgatgc aggaccgact gcagcacagc
     5941 ttcgccgcgg cctcgcgtca ccagcttcag ggcgcgctgc tgttcatcga tctcgatcac
     6001 ttcaaaaacc tcaacgacac caaaggccat aatttcggtg acagcctgct gcacatggtg
     6061 gcggaaaggc tgcaatcctg cgtgcgcgag ggcgataccg tggcgcggct gggaggcgac
     6121 gaattcgtcg tcatcctcga gaatctgagc gagaacgccg agcaggccgc ggccttggcc
     6181 gaagccgtgg gtgacaaggt cctcgccact ctgggccagc cctacctgat cggcggtgac
     6241 gagtatcact gcaccgcgag tatcggcgtc agcctgttca agaagcatga ggccacggtc
     6301 gaagagctgg tccggcgcgc cgataccgcc atgtacgaag ccaagaacag cggccgcaac
     6361 acactgcgct tctacgaccc ggcgatgcag gccgcgctcg aagcgcgcat gcagctcgag
     6421 agcgatttgc gtcatgcgct gacagccgat cagttcaagc ttttctacca gccgcaggtc
     6481 aatacgcaag gcgaggtgtt cggcgccgaa gtgttgatcc gctggatatc gccggagaaa
     6541 ggcttgatcc cgcccatgca gttcatcccg ctggcggaag agaccggcct catcgtcccc
     6601 atcggcaact gggtgctgca ggcggcttgc gatcagctgc tgcaatggcg caaggatccg
     6661 cgcaccgcac ggctgcagct tgcggtcaac gtcagcgccc ggcagttcca ccagcctgat
     6721 ttcatcgagc aggtgctgca gatcctcatc gacaagaagg tcgatcccag ccgcctcaag
     6781 ctggaactga ccgagagcct ggtgctggac gatatcaatt ccgccatcac caagatgaac
     6841 gcgctgaaag aggccggcat cagcttctcg atggacgatt tcggcaccgg atattcatcc
     6901 cttacctacc tcgcccaatt gccgctgacg catctcaaga tcgatcagt
//
LOCUS       DATMTW010000006         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_436378, whole genome shotgun sequence.
ACCESSION   DATMTW010000006 DATMTW010000000
VERSION     DATMTW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436378"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1478)
                     /gene="tyrS"
                     /locus_tag="VN084_00090"
     CDS             complement(282..1478)
                     /gene="tyrS"
                     /locus_tag="VN084_00090"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWT27882.1"
                     /translation="MTQLNESLAIIKRGSDELLIEQELVDKLKTGKPLRVKAGFDPTA
                     PDLHLGHTVLLNKLRQFQDLGHQVLFLIGDFTGMIGDPTGKSATRPPLTAEQVQDNAK
                     SYAEQVFKILKPEQTEVVFNSEWLNALGAAGMLKLAATHTVARMLERDDFHKRYHSNQ
                     PIAIHEFHYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHHGQPQQVVLTMPLL
                     EGLDGVNKMSKSLGNYIGVAEPANIIFSKIMSISDELMWRYIDLLSFESLQTIAQWKR
                     DIEAGANPRDTKVKFAQEIVARFHSRAAAVEALADFELRAKGGIPDDVPEMTLNIEDD
                     TVGIAQLLKLAGLVASTSEAFRAIEQGGIKLDGERVMDKGVALQRGQTVVAQVGKRKF
                     ARITIA"
     gene            1656..>2450
                     /locus_tag="VN084_00095"
     CDS             1656..>2450
                     /locus_tag="VN084_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HWT27883.1"
                     /translation="MLAISSIPLFGIYAAFGIAPQTVVEDIPVATVIEQLTLPQAPLA
                     SDLSTAATGQFWQTDIVRRDDTLSSLMARLNITNPDALSFLRTSPSASSLASQLIPGR
                     SIQAQTTADGDLLTLEYPNGNATLLHVERTDSGYRAEIHDASLQTHSVLKTAEIKSSL
                     FGATDEAGIPDAIAIQLAEIFSSDIDFHSDLRKGDRFVVVYEASYSNGELMKTGQVLA
                     AEFVNDGKTYRAVRYRDPDGQVGYYTPEGRSLHKSFLRSPLEFTRIS"
BASE COUNT          516 a          733 c          647 g          554 t
ORIGIN      
        1 ttcaaggtgt ttaagcactt gtatcttgag tcgcccaaca tcccgaagaa tgtcaggtcg
       61 tccacaacca caagcactca cacctatcga ttgttcatat tgttaaagag cggtcgctac
      121 gtcagtaacg agaggggcgc attatacaga ccgtattgat tcggtcaagg gttcaacgga
      181 aaaatttgct acctaccgtt tcgtcctgca cgcccgtcgt tcaagcgagc cgcgcattct
      241 acaggcccca agaaattggt caataggctg cgcgaaaaaa tttaagcgat cgtgatgcgg
      301 gcgaacttgc gcttgcctac ctgcgccact acagtctggc ctctttgcaa ggcgacgccc
      361 ttgtccatta ccctttcccc gtccagcttg atgccgcctt gttcgatggc gcggaaggct
      421 tccgacgtgc tggcgaccag acccgccagc ttcagcaact gagcgatgcc aacggtatcg
      481 tcttctatat tcagcgtcat ttccggcaca tcgtccggga tgccaccctt ggcgcgcagc
      541 tcgaagtccg cgagcgcctc cacagcggcc gcgcgggaat ggaagcgtgc cacgatctcc
      601 tgggcgaact tcaccttggt gtcgcggggg ttggcaccgg cttcgatatc tcgcttccac
      661 tgggcgatcg tctgcagcga ctcgaaagac agcaggtcga tataccgcca catcaactca
      721 tcggagatcg acatgatttt gctgaagatg atattggcag gctctgccac accgatatag
      781 ttacccaacg acttcgacat cttgttgacg ccgtccaggc cttccagcaa aggcatggtg
      841 agaaccactt gctgcggctg accgtgatgc ttttgcaact cacgccccat gagcaggttg
      901 aacttttgat cagtaccacc caactccacg tcggccttca gcgccacgga gtcataaccc
      961 tgcagcaacg gatagtggaa ctcatggatc gcgatgggct gattgctgtg atagcgcttg
     1021 tggaaatcat ctcgttccag catgcgtgcg acggtatggg tggccgccag tttcagcatg
     1081 ccggccgcgc ccagtgcatt gagccattcg gagttgaaga ccacttcggt ctgctcgggc
     1141 ttcaatatct tgaatacctg ctcggcatag gatttggcat tgtcctgcac ctgctcggcg
     1201 gtcaggggtg ggcgggtcgc gctcttgccc gttgggtcac cgatcatgcc ggtgaaatcg
     1261 ccgatgagga agagcacctg gtgcccgaga tcctggaact ggcgtaactt gttgagtaac
     1321 acggtatggc cgaggtgcag gtccggcgcg gtaggatcga agccagcctt gacgcgcaac
     1381 ggcttgccgg tctttaactt gtcgaccagc tcctgttcga tcagcagttc gtcactgcca
     1441 cgcttgatga tggcgagact ttcattcagt tgcgtcatgc ttatctatgt ccttgcgtac
     1501 ttatcgcttt caatctgcag ggtttttctg ttaggattgc ccgtccgatc cagttacccg
     1561 aggtagtgcc ggtgcagcag aacgaaccta ctcagaaaca aagtattcta gcgcaaaacc
     1621 ccagttttaa agaacgtaag ctccgtctgc gttggttgtt agcgatctcc agcattccgt
     1681 tattcggcat ctatgccgct tttggtatcg ctccacagac tgtcgtcgaa gacatcccgg
     1741 tcgccaccgt catcgaacag ctcaccctgc cgcaagcgcc gctcgcgtcg gatctgtcga
     1801 ccgcagcgac cggccaattc tggcagaccg atatcgtacg tcgcgatgac accctgtcca
     1861 gcctcatggc gcgtctcaac atcaccaatc cggatgcgct gagcttcctg cgtaccagcc
     1921 cctctgccag cagcctcgct tcacagctga tccctgggcg cagcatccag gcgcaaacta
     1981 cagcggacgg tgacttgctg accctggaat atcctaacgg caatgccacc ctgctacacg
     2041 tcgaacgtac ggatagcggc taccgcgccg aaattcatga cgccagcctg caaacgcaca
     2101 gcgtgctcaa gaccgctgag atcaagagct ccttgttcgg ggccactgac gaagccggca
     2161 ttcccgatgc gatcgccatc cagcttgccg agatcttttc ctcggatatc gatttccata
     2221 gcgatctacg caaaggcgat cgcttcgtcg tggtctatga agccagctac agcaatggcg
     2281 agttgatgaa gaccggacaa gtgctggcgg cagagttcgt caatgacggc aagacctatc
     2341 gcgcagtacg ctaccgcgat ccagatggcc aggtcggtta ttacacccct gaaggcagga
     2401 gcctgcataa gtctttcctg cgctctccac tggaattcac gcgcatcagc
//
LOCUS       DATMTW010000007         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_537100, whole genome shotgun sequence.
ACCESSION   DATMTW010000007 DATMTW010000000
VERSION     DATMTW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537100"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN084_00100"
     CDS             <1..311
                     /locus_tag="VN084_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWT27884.1"
                     /translation="AVWTVEFQLPQAAIKAEYIPGNQYARVERQDANVWGFITRMHKG
                     VGANVAWVLLADTIAGAMLLLSITGVLLWTKMRGSRLAMAGLIGGSLVATILTTLSML
                     "
     gene            complement(363..1658)
                     /locus_tag="VN084_00105"
     CDS             complement(363..1658)
                     /locus_tag="VN084_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT27885.1"
                     /translation="MLFITHDRRFLDRLATRIVELDRGQLTEFIGNFTQYQAKKEELL
                     AVEATHAAKFDKVLAQEEAWIRQGIKARRTRNEGRVRRLEKLRLERAARRERQGTVKL
                     SLDAGERSGKLVAELDHVEKAYGDKVLIRDFSTRILRGDRIGLLGPNGVGKTTLLKLI
                     LGEEAPDSGHIQRGTNLNIAYFDQMREQLDEEATLADTISPGSEFVQIGNERKHVISY
                     LEDFLFPPQRARSPVKSLSGGERNRLLLARLFANPANVLVLDEPTNDLDIETLELLES
                     LLQEFEGTLFLVSHDRAFLENTVTQVIAFEGDGVLTEFGGGYDDWRRFTAQRAEAARS
                     EARSEKSRPAAAKSATSTAAKPGADKLSFKEIKELEALPDRIAAAEAEVAAINHQLAD
                     SNLYRSQPEQVKTLQARLQTLEADIETLMLRWEALESRR"
     gene            complement(1710..2279)
                     /locus_tag="VN084_00110"
                     /pseudo
     CDS             complement(1710..2279)
                     /locus_tag="VN084_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2307..>4356)
                     /locus_tag="VN084_00115"
     CDS             complement(2307..>4356)
                     /locus_tag="VN084_00115"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWT27886.1"
                     /translation="PEYAQSPALPTRRPGLMRTLLLWLASLLLLAAISLGVAFAVCEA
                     KGWPFLRAPAETFLSQSLQREVRIAEPFRLKLLGSVRLDVGGLWIAAPKGFKAPHFVD
                     MRDAELELRYRDLWQLRTSDLLRIRSLKVGSIDAQLIRLDSGKASWQFDQDETAPDKP
                     LPMIETLVVRNGRALLNDTPSQADLDVRFKTEEGSAQQQARSHVSLSGRLRGKPIKGD
                     LRTQGLLPVATQDADSPPIASAGWVDYAGVRVDFDGSISDLFGKQDIQGKFAAKGASL
                     SILGDLVNSTLPTTDPFKLNGRIRKADAVWQVDVASAEVGSSQLSGTFSYDPRPQRPL
                     LVGKLNSKNFVLADLAPAFGTRNPDGSKAKAPDGKTLPDRPLDLPSLNKMDAKVEIDL
                     AHVDLGSAFARPISPLKGKLTLDDGKLALTDLDARTADGSVSGLVAIDAAKGADHGKA
                     QWQIDLNWQDIDVEKWLQVSQDRKQAAKEDGQREPPPAYVTGTLNGTSKLRGEGASTA
                     ALLSSLDGEVSFFIRQGTISHLVVEVLGLDIAQGLGILLTKDQSLPMQCAVMDFDARQ
                     GVITPKVALLDTPVTVVVIDGKVNLATEQLDLRLAAKPKNVSPLTVRSPIRVTGTFAD
                     PKASPESGPIAARVAGAVALAFVNPLAAILPFVDLGGAGKEASPCNKTLSELSQKQAR
                     "
BASE COUNT          706 a         1339 c         1435 g          876 t
ORIGIN      
        1 cagcagtgtg gaccgtggaa ttccagctgc cgcaagccgc catcaaggcc gaatatatcc
       61 cgggcaatca atatgcgcgc gtcgagcgtc aggacgccaa cgtctggggc ttcatcacgc
      121 gcatgcacaa gggcgtaggg gccaacgtcg cctgggtgct gctcgccgac accatcgctg
      181 gcgccatgct gctgttatcc atcaccggcg tgttgctgtg gaccaagatg cgcggttcgc
      241 ggctggccat ggctggcttg atcggcggct cgttggtcgc cactatcctc accaccttgt
      301 ccatgctctg aggcaagcga taccgaaaag caggggtcac ggcccctgtt gtcggtatgc
      361 gcctagcgcc gactttccag tgcctcccag cgcagcatca gggtctcgat atcagcttcg
      421 agcgtctgca ggcgggcttg caacgttttc acctgttcag gttggctgcg gtataggttg
      481 ctgtcagcca gctggtggtt gattgcagcc acctcggcct ccgctgccgc gatgcgatcg
      541 ggcaaggctt ccagttcctt gatctccttg aaactcagtt tgtcggctcc cggtttggcg
      601 gctgtgctgg tcgctgattt ggccgccgct ggccgtgact tttccgaccg tgcctcgcta
      661 cgcgccgctt ccgcacgctg ggcggtgaaa cgacgccaat cgtcgtaacc gccgccgaac
      721 tcggtgagca cgccgtcgcc ttcaaaagcg atgacctggg tcaccgtgtt ctccaggaag
      781 gcgcggtcat ggctgaccag gaacaacgtg ccttcgaact cctgcagcag gctctccagt
      841 agctccagcg tctcgatatc gagatcgttg gtcggttcgt ccaacaccag cacattggct
      901 ggattggcga acaggcgagc cagcaacagg cggttgcgtt cgccgccgga aagcgatttc
      961 accggcgagc gggcgcgctg cggcgggaac aggaagtctt ccaggtagct gatgacgtgt
     1021 ttgcgttcgt tgccgatctg tacgaattcg gaaccggggc tgatggtatc ggccagcgtg
     1081 gcctcctcat ccagctgttc gcgcatctgg tcgaagtagg cgatgttgag gttggtgccg
     1141 cgctggatat gcccgctatc gggtgcctct tcgccgagga tgagcttgag cagggtggtc
     1201 ttgccgacgc cgttggggcc gagcaggccg atgcggtcgc cgcgcaggat gcgggtgctg
     1261 aaatccctga tcagcacctt gtcgccgtag gctttctcta cgtggtccag ttcggcgacc
     1321 agcttgcctg accgttcccc ggcatccagc gatagtttga ccgtgccctg gcgctcgcgt
     1381 cttgcagcgc gttcgaggcg cagcttctcc agccggcgca ccctgccttc gttgcgcgtg
     1441 cggcgtgcct tgatgccctg gcggatccag gcttcttcct gcgccagcac cttgtcgaac
     1501 ttggcggcat gcgtcgcttc caccgccagc agctcttcct tcttggcctg atattgcgtg
     1561 aagttgccga tgaattcggt gagctggcca cgatcgagtt cgacgatgcg cgtggccagg
     1621 cgatccagga agcgccggtc gtgggtgatg aacagcacgc tgccgttgaa gcccagcagc
     1681 aggtcttcca gccactcgat ggcttgcagg tcgaggtggt tggtcggctc gtccagtaac
     1741 aacacttcgg gctcggcgac caaggcgcgg gccagggcga cacgcttgcg ccagccgccg
     1801 gagagcttgc tgatttcggc gtcagcatcc aggccaagct tgctcaacac ggtctctacg
     1861 cgtgcctgcg aatgccagcc ctgtccggcg tcgagcgcat gctgcagcgt ctgcatgcgg
     1921 gccatgagcg catcggtatc agcggcgggg tcggcgaggg catgtgtcac ttcgtggtac
     1981 tcgaccagcg ttctttgcaa tgccccaaga ccttcggcaa cggcttcgta gacggtatgg
     2041 tccggctgca ggctgggttc ttgcggcaca taggcgacgc gggcggcggg cgcgacccaa
     2101 agcgaaccgt cgtcgagctt gctttcgccg gcaatgactt tcagcaggct ggatttgccg
     2161 gtgccgttgc ggccgatgag gccgacgcgt tcgcccgggt ctagctggag gttcgcttgg
     2221 tcgagcaggg gatggtggcc gtaagccagg ctggcttgat tgagggtaat aaggggcatc
     2281 cggctatttt accagccgga ccgggtttat cgcgcctgtt tctggctcaa ctcggacaag
     2341 gtcttgttgc agggcgatgc ctccttgccg gcaccgccga gatcgacgaa gggcaggatg
     2401 gcggccagcg gattgacgaa ggccaacgcg acggcgcccg cgacccgggc ggcgatcggc
     2461 ccgctttcag gcgaagcctt ggggtcggcg aaggtaccgg tgacgcggat cggcgagcgc
     2521 acggtcagcg gtgagacatt cttgggtttg gccgcgagcc tgagatcgag ctgttccgtc
     2581 gccaggttga ccttgccgtc gatgacgacc acggtgaccg gcgtatcgag cagcgccacc
     2641 ttgggcgtga tgacgccctg gcgggcatcg aaatccatga ccgcacattg catgggtagc
     2701 gactggtctt tggtcagcag gatgccgagc ccttgcgcga tatcgaggcc gagcacctcg
     2761 accaccagat gtgagatcgt gccctggcgg atgaagaacg acacctcacc gtcgagtgaa
     2821 ctcagcagcg ccgcggtcga cgcgccttcg ccgcgcaact tgctggtgcc gttcaatgtg
     2881 ccggtgacat aggctggcgg cggctcgcgc tggccgtctt cctttgcggc ctgcttgcgg
     2941 tcttgcgaga cctgcagcca tttctcgaca tcgatatctt gccagttaag gtcgatctgc
     3001 cactgggcct tgccgtgatc tgcgcccttg gcggcatcga tcgcgaccag gcccgagacg
     3061 ctgccatcgg cggtgcgtgc atcgagatca gtcaatgcca gcttgccgtc gtcgagcgtg
     3121 agcttgccct tgagcggcga gatcggtcgg gcaaaggcgc tgccaagatc gacatgcgcc
     3181 aggtcgatct ccacctttgc atccatcttg ttgagcgagg gcagatccag cggccggtcc
     3241 ggcaaggtct tgccgtctgg cgcttttgcc ttgctgccgt ccgggttgcg tgtcccgaag
     3301 gctggggcca ggtctgccag cacgaaattc ttgctattga gcttgcccac cagcaatggc
     3361 cgctgcgggc gtggatcgta gctgaacgtg ccggaaagct ggctgctgcc gacttcggcc
     3421 gatgccacgt cgacctgcca gaccgcatcg gccttgcgga tcctgccgtt gagcttgaac
     3481 gggtcggtgg tcggtagcgt gctgttgacc agatcgccga ggatactcaa cgacgcgccc
     3541 ttggctgcga acttgccctg aatgtcctgc ttgccgaaga ggtcggagat gctgccgtca
     3601 aaatccacgc gcaccccggc gtaatcgacc cagcctgcgg atgcgatggg cggcgaatca
     3661 gcgtcctggg tcgctaccgg cagcaggcct tgggtacgca gatcgccttt gatcggcttg
     3721 ccgcgcaacc gaccggagag cgagacatgc gatcgtgcct gctgctgggc gctaccttcc
     3781 tcggtcttga agcgcacatc gagatcggcc tggctggggg tgtcgttgag tagcgcacgg
     3841 ccgttgcgca ccaccagggt ctcgatcatc ggcaggggtt tgtctggggc ggtctcgtcc
     3901 tggtcgaatt gccagctggc cttgccgctg tcgaggcgga tcagttgggc gtcgatgctg
     3961 ccgaccttga gcgaacggat acgcaacaga tcgctagtgc gcagctgcca caggtctcga
     4021 tagcgcagtt cgagctcggc atcccgcatg tcgacgaagt gcggcgcctt gaagcccttg
     4081 ggtgccgcga tccaaagccc gcccacatcc agccgcacgc tacccagcag cttgagccgg
     4141 aaaggctcgg cgatacgcac ttcgcgttgc aagctctggc tcaggaaggt ttcggccggt
     4201 gcacgcagga aaggccagcc cttcgcctca cagacggcaa aggcgacacc gaggctgatg
     4261 gccgcgagca atagcaggct ggccagccac agcagcaggg tgcgcatcaa gcccggccgc
     4321 cgggtcggca gcgcagggct ttgcgcatat tcaggg
//
LOCUS       DATMTW010000008         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_335902, whole genome shotgun sequence.
ACCESSION   DATMTW010000008 DATMTW010000000
VERSION     DATMTW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335902"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..426)
                     /locus_tag="VN084_00120"
     CDS             complement(187..426)
                     /locus_tag="VN084_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27887.1"
                     /translation="MKPENACHRVVLAGAAGCRVVYCEDCQVAELEIGAMSLRLETPA
                     FDTLAAVMQAAETRLARLKAARLEDGFIARSGNVH"
     gene            complement(436..627)
                     /locus_tag="VN084_00125"
     CDS             complement(436..627)
                     /locus_tag="VN084_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HWT27888.1"
                     /translation="MALPATPELMGESAPDKASCAAADLLRWSSHALLAGKREAVIEH
                     HGVEYRLRLTGQGKLILTK"
     gene            complement(806..1423)
                     /gene="rpsD"
                     /locus_tag="VN084_00130"
     CDS             complement(806..1423)
                     /gene="rpsD"
                     /locus_tag="VN084_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWT27889.1"
                     /translation="MSRFTGPRLKVMRALGVELPGLSRKSIESRPTPPGQHGARAVRR
                     KKSDFGVKLMEKQKLRFNYGLSERQLRRLMMDARRGQAPTGETLLSLLERRLDNVVYR
                     AGYAPTIAAARQLVNHRHVLLNGKLASIPSMRLKVGDEVTLKSSSQQLPMVLETLKDM
                     PLTRPEWLVWDAQGQRARIMHLPAAEDEPFPVDVQQVVEYYANRL"
     gene            complement(1445..1726)
                     /locus_tag="VN084_00135"
     CDS             complement(1445..1726)
                     /locus_tag="VN084_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27890.1"
                     /translation="MNTENTSHHDDDLPDASAVMASLCCVAARYAASPSLELATLAAE
                     LSRKLTAPRYAESRLVREVAQRLVTQWQSIVETRSFAFVSVVPASAALH"
     gene            2046..>2370
                     /locus_tag="VN084_00140"
     CDS             2046..>2370
                     /locus_tag="VN084_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27891.1"
                     /translation="MLKVIIAVIVSAIVIYLGNQHGIVVWMGAAILCAILESTLFNNI
                     VLFIPAAVGGAGAFGQFFMNDAQTASFAAVAIGVVSLLLLFKEMTEDKKVVEEIKREN
                     MKKLRN"
BASE COUNT          521 a          685 c          671 g          493 t
ORIGIN      
        1 gtcgtctgcc gggcaagcgc caggtagaac agtccgacat gcaaaaggat caccagcgcc
       61 agggtcgacg cagacaggcg accccgcgcc gcaggcgtgg attgaggaac agcgggacac
      121 aggctgcgca cgttgcgggc atcagggcgc ggccgaacgg cttgaaggtt gagggcgtat
      181 atagtttcag tggacatttc cggacctagc gatgaatcca tcctctaatc gagccgcctt
      241 cagtctggcg agacgtgttt cggccgcttg cattaccgca gccagggtat cgaatgccgg
      301 cgtttcaaga cgcaggctca tggcgccgat ctccagctcc gcgacctggc aatcctcgca
      361 atagacgacg cgacagcctg ctgcgcctgc gaggacgaca cgatgacatg cgttttccgg
      421 tttcatgtgc atcgcttact tcgtcaggat cagcttgccc tggccggtca gccgcaaccg
      481 gtattccacg ccatggtgct cgatcaccgc ctcacgcttg cccgccagca acgcgtggct
      541 gctccagcgc aataggtcgg ctgctgcaca gctcgcctta tcgggcgcgc tttcccccat
      601 caactcaggt gttgcaggta atgccatcag ggtgtcagtc atcgtggcct caggcatgct
      661 catggctgtg aatgttgatg ataacgattc tcatttgtga agtcaatgct atgtccatgt
      721 attcaattaa atttaggatc gaacttgggc aaaaaaaggc cgctcgcgcg gccaagatga
      781 ggagaaacaa cttggtacaa cacctttaca ggcgattggc gtaatactcc accacctgtt
      841 gcacatccac cggaaacggc tcatcttcgg ccgccggcaa gtgcatgatc ctggcccgct
      901 ggccttgtgc gtcccagact aaccattccg ggcgtgtcag cggcatgtcc ttgagcgttt
      961 ccagcaccat gggcaattgc tggctggacg acttcagcgt cacctcatca ccgaccttga
     1021 gccgcatgga tgggatgctg gcgagcttac cgttgagcaa gacatgacga tgattgacca
     1081 gctgccgtgc cgctgcgatg gtgggcgcat aaccggcacg ataaacaacg ttgtcgagac
     1141 gtcgctcgag caatgacagc aacgtctcgc ctgtcggcgc ctgaccgcga cgcgcatcca
     1201 tcatcaggcg gcgcagctgg cgttcggata agccatagtt gaaacgcagc ttctgctttt
     1261 ccatgagctt gacgccgaaa tccgatttct tgcgtcgcac agcgcgcgcg ccgtgctggc
     1321 ctggcggggt cgggcgcgat tcgatacttt tgcgcgatag cccaggcagc tcgacaccaa
     1381 gggcacgcat cactttcaat ctagggccgg taaagcggga catacgaact cctgattcat
     1441 tgattcaatg cagcgccgcg cttgcgggga ccacgctgac gaaagcgaaa ctgcgcgttt
     1501 cgacgatgga ttgccattgg gtcaccagcc gttgcgccac ttcacgcacc agacgagatt
     1561 cggcataacg cggcgccgtc agcttgcggg acaactccgc tgcaagtgtc gccaattcca
     1621 gcgaagggct tgccgcatag cgggctgcga cacagcacaa ggaagccatc acggcgctgg
     1681 catccggcag gtcgtcgtca tgatgacttg tattctcggt gttcatggca aggcctcagt
     1741 taacatgcaa caaatgataa tgattctcat ttaataagtc aaacccgaat atgcttggcg
     1801 gctgtcgaaa gcctgacagg cctgtagaaa tatcgactgc atgtcgacag caagatagcg
     1861 ccgcaacatg gcctgccgat tgccgacgat gagaacaaat ccatatacat cagcgacttg
     1921 aatttcaatt acggcctatg tcgaccgttc gacgcccggg ggaacgggaa ttgcaaacac
     1981 ctatgacaag acgtgtcgaa tatttcggca cttagttgcc agacctgatc cttgaggaat
     2041 cgaccatgct aaaagtcatc atcgccgtta tcgtctccgc catcgtcatc tacctgggca
     2101 accagcatgg catcgtcgtc tggatgggcg ccgcgatcct gtgcgcgatc ctggagagca
     2161 cgctgttcaa caacatcgtg ctgttcatac cggctgcggt cggtggcgcc ggcgcgttcg
     2221 gccagttctt catgaatgat gcgcagaccg cctcgttcgc cgccgtggcc atcggtgttg
     2281 tcagcctgct gctgctgttc aaggagatga ccgaggacaa gaaggtcgtg gaagagatca
     2341 agcgagaaaa catgaaaaag ctgcgtaatt
//
LOCUS       DATMTW010000009         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_168278, whole genome shotgun sequence.
ACCESSION   DATMTW010000009 DATMTW010000000
VERSION     DATMTW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168278"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VN084_00145"
     CDS             complement(<1..287)
                     /locus_tag="VN084_00145"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWT27892.1"
                     /translation="MLKTRILTSTVLIPGFLAALFMLPDFYWSLLMLVLIGIGLWEWA
                     VMSRFSPWGRVAYVGTAVLACVAVVVGAGDAILYLQEYAIFWGILAAAVFWL"
     gene            complement(280..1059)
                     /locus_tag="VN084_00150"
     CDS             complement(280..1059)
                     /locus_tag="VN084_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWT27893.1"
                     /translation="MAFFSSSTQTVPPISAVPRHIAVIMDGNGRWAKKRFLPRIAGHK
                     RGVEVVRDMVKECATLGVEYLTLFAFSSENWRRPQEEVSFLMGLFMEALKYEVSKLHQ
                     NNIRLVMIGDRSRFDDALRAQIDEAERTTADNTGLVLTIAANYGGRWDLMQAMNRMQM
                     AHPERNGQFEEQDLAPYLSMHYAPEPDLFIRTGGEERISNFLLWQLAYSELYFTDTLW
                     PDFNQKAFNQAIKSYQSRERRFGRTSEQLGDEQQKTQAKHA"
     gene            complement(1120..1677)
                     /gene="frr"
                     /locus_tag="VN084_00155"
     CDS             complement(1120..1677)
                     /gene="frr"
                     /locus_tag="VN084_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWT27894.1"
                     /translation="MLNDLKKNAEQKMQKSLDALKNDLGKIRTGRAHTGLLDHVMVEY
                     YGSMVAVNTVANVTLGDARTINVQPYEKSMIGKVEKAIRDGDLGLNPATNGDVIRVPM
                     PMLTEERRKDLIKVVRAEGENAKVSIRNVRRDANDALKKLVKDKEISEDDERRAQDEI
                     QKLTDKSVAEVDKMLQVKEADLMAV"
     gene            complement(1722..2438)
                     /gene="pyrH"
                     /locus_tag="VN084_00160"
     CDS             complement(1722..2438)
                     /gene="pyrH"
                     /locus_tag="VN084_00160"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWT27895.1"
                     /translation="MSAPAYKRILLKLSGEALMGDDSYGINRATIFRIVNEVKEVVDL
                     GVQVGVVIGGGNIFRGVAPAAEGMDRATADYMGMMATVMNALALQDAMKHVGLVSRVQ
                     SALNIEQVAEPYIRGKAIRYLEEGRVVIFGAGTGNPFFTTDTAAALRGMEINADIVLK
                     ATKVDGIYTDDPKTNPDAMRYKTVSFDEAINKNLKVMDATALTLCRDQKLPITVFSIF
                     KQGALKRVVMGEDEGTSVLP"
BASE COUNT          495 a          727 c          694 g          555 t
ORIGIN      
        1 agccagaata cggcggcggc gaggataccc cagaatatcg catattcctg caggtaaagg
       61 atggcatcgc ctgcaccaac gacaaccgcc acacaagcca gcactgccgt accgacataa
      121 gcaaccctgc cccatgggct gaagcgcgac atgaccgccc actcccagag gccgatgccg
      181 atcagcacca gcatgagcaa ggaccagtag aaatccggca gcatgaacaa tgcggcgaga
      241 aagcccggga tcagtacggt ggaagtcagg atgcgggttt taagcatgtt tggcctgtgt
      301 cttttgctgc tcgtcaccca gttgctcgct ggtgcgaccg aagcggcgct cgcgactctg
      361 gtaggacttg atcgcctgat tgaaggcttt ttggttgaaa tccggccaca gggtgtcggt
      421 gaaatagagt tcgctgtagg caagctgcca gagcaggaaa ttactgatgc gctcttcgcc
      481 gccggttcgg ataaaaagat caggctccgg cgcgtagtgc atggagaggt aaggcgcgag
      541 atcctgctcc tcgaactggc cgttgcgttc agggtgagcc atctgcatgc ggttcatggc
      601 ttgcatcagg tcccagcgac caccgtaatt ggctgcgatg gtcagcacca gcccggtgtt
      661 gtctgcggta gtgcgttcgg cttcatcgat ctgggcacgt aaagcgtcat cgaaacggga
      721 ccggtcgccg atcatgacca agcggatgtt attctggtgc agcttgctga cctcatactt
      781 cagggcttcc atgaacaggc ccatgaggaa agagacttct tcttgcggac gccgccagtt
      841 ctcgctgctg aatgcgaaca gggtcagata ctcgacaccg agtgtggcgc attccttgac
      901 catgtcgcgc accacctcga caccacgctt gtgccctgcg atcctcggca ggaaacgctt
      961 cttagcccaa cggccattgc cgtccatgat caccgcgatg tgacggggaa ccgcagaaat
     1021 cggggggacc gtttgcgtgg agctggaaaa aaaggccaat caatgctcca tgttcaaaga
     1081 caaaagctgg gaacctgaat gatagcgttt cgcggccgct cacacggcca tgagatccgc
     1141 ttctttgact tgtaacatct tgtcgacttc agccactgac ttgtcggtca atttctggat
     1201 ctcgtcctga gcacggcgct cgtcatcttc actgatctct ttatccttga ccagtttctt
     1261 caacgcatcg ttcgcatcgc gacggacatt gcggatcgac accttggcgt tctcgccctc
     1321 ggcacgcacg accttgatca ggtccttgcg tcgctcctcg gtgagcatgg gcatcggcac
     1381 ccggatgacg tcgccgttgg ttgccggatt gagacccaga tcaccgtcgc ggatggcttt
     1441 ctccaccttg ccgatcatgc tcttctcgta aggctggaca ttgatggtgc gggcatcgcc
     1501 caacgtaacg ttggcgacgg tgttgaccgc caccatcgac ccgtaatatt cgaccatgac
     1561 atggtcaagc agccccgtat gggcgcggcc cgtgcggatc ttgcccaagt cgttcttgag
     1621 cgcgtccagg gacttctgca tcttttgttc ggcgtttttc ttaaggtcat tgagcatatt
     1681 attctccgtc gtccttgatc acgataatct ggcaggggtg cctaaggcag cacgctggtg
     1741 ccttcgtcct cgcccatcac gacacgcttg agcgcgccct gcttgaagat gctgaatacc
     1801 gtgatcggca atttctggtc acggcacagt gtcagcgcgg tggcatccat caccttgagg
     1861 ttcttgttga tcgcttcgtc gaagctgact gtcttgtaac gcatggcgtc tggattggtc
     1921 ttggggtcat cggtgtagat gccgtcgacc ttggtcgcct tgagcacgat atcggcattg
     1981 atctccatgc cgcgcagcgc cgctgcggta tccgtcgtga agaacgggtt gccggtgccg
     2041 gcaccgaaga tcaccacacg cccttcttcc aggtagcgga tcgccttgcc ccggatatac
     2101 ggctcagcga cttgttcgat attcaatgcg gattgtaccc ggctcaccag cccgacatgc
     2161 ttcatggcgt cctgtagcgc aagggcgttc atgacggtcg ccatcatgcc catatagtct
     2221 gcagtggcac ggtccatgcc ctcggctgcc ggcgcgacac cgcggaagat attgccgccg
     2281 ccgatcacca cgccaacctg cacacccagg tctacgactt ccttgacctc attgacgatt
     2341 ctgaagatgg tcgcacggtt gatcccgtaa ctgtcatcgc ccatcagggc ctcaccagag
     2401 agcttgagca gaattcgctt ataggcaggt gcagacatag ggctcctcga gttgggcaag
     2461 acgcagcctg c
//
LOCUS       DATMTW010000010         6254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_604579, whole genome shotgun sequence.
ACCESSION   DATMTW010000010 DATMTW010000000
VERSION     DATMTW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604579"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1202)
                     /gene="prpC"
                     /locus_tag="VN084_00165"
     CDS             complement(54..1202)
                     /gene="prpC"
                     /locus_tag="VN084_00165"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HWT27896.1"
                     /translation="MNAVVEPKRKKGVALAGVAAGTTAICTVGHSGNDLHYRGYDILE
                     LAKHATFEEVAYLLIHGELPNRQQLADYHKKLKQLRALPEALKKVLEQIPASAHPMDV
                     MRTGCAMLGTLEPEAADRNIQGSKDLGDRLISCFGSILLYWYHYSHNGKRVEVVTDDD
                     SIAAHFLHVLHGKKPSELHIKAMNTSLVLYAEHEFNASTFTARVIAGTLSDMYSCITG
                     AIGALRGPKHGGANEAAMEIIERYKTPDEAEADIVRRIANKEIVIGFGHPVYTIADPR
                     NVAIKEVSRSLCQDAGDMHLFDISDRLETVMWREKKMFPNLDWYSASSYHMMGIPTGM
                     FTPIFVISRTTGWVAHVIEQRVDNKIIRPNAEYTGPEDRAYVPITQRQ"
     gene            complement(1206..2102)
                     /gene="prpB"
                     /locus_tag="VN084_00170"
     CDS             complement(1206..2102)
                     /gene="prpB"
                     /locus_tag="VN084_00170"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HWT27897.1"
                     /translation="MSQLHSAGARFRAALAAEKPLQIIGAINAYHARLATQTGYKAIY
                     LSGGGVAAGSLGLPDLGISTLEDVLIDVRRITDNVETPLLVDIDTGFGGAFNIARTIK
                     AMTKAGAAAVHIEDQVQSKRCGHRPNKAIVSQVEMVDRIKAAVDAKTDPDFVIMARTD
                     ALAVEGLQSAIDRACACVEAGADMIFPEAITSLQMYKQFAEAVKVPVLANITEFGSTP
                     LFTVQELASVNVGMVLYPLSAFRAMNAAALKVYQAVRRDGTQKNVVDTMQTRMDLYKY
                     LDYHAYEEKLDQLFASQQGTEE"
     gene            complement(2125..3111)
                     /locus_tag="VN084_00175"
     CDS             complement(2125..3111)
                     /locus_tag="VN084_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWT27898.1"
                     /translation="MSVHPNEALFAGEKPFPVIPSCEHFAGSEKLILKALSLQDSLGP
                     IFDITCDCEDGAASGQEREHAEMIVRVLNSDANKHRMAGARIHDYTHPAWKQDIDILV
                     GGAGQVLSYITIPKCTRAAQAAEMIAYLQKVATSHGIQREIPVHVLIETHGALHEVYE
                     IAALPWLQVLDFGLMDFVSGHHGAIPASAMRSPGQFEHRLLARAKADVVAAALAHGVV
                     PAHNVTLDLKNTETTYSDASRARNEFGFMRMWSIYPTQIQAIVDAMKPDYSEVEDAAN
                     ILLAAQQAEWGPIQYAGELHDRATYRYFWEVLQKARITGVGIPQNATHAFFA"
     gene            complement(3123..4181)
                     /locus_tag="VN084_00180"
     CDS             complement(3123..4181)
                     /locus_tag="VN084_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWT27899.1"
                     /translation="MTTASGNFFEHFRLGQEIHHATPRTITEGECALYIALTGARQPL
                     HCATPVAQALGYRDKPVDDLLAFHIAFAKTVPDISVNAVANLGYADVRFLRPVYVGDT
                     LFTTSTVIGLKQNSNGKSGVVYVRSESRNQHSEVVLSWVRWVMVHKRDQDAPAPETVI
                     PELPAVVGVKQLAIPRFLQPTQLASHATGSTRLWDDYQAGERIDHPAGMTVDDSDHTL
                     ATRLYQNNARLHFDALMMQDSAFGQRLMYGGHVISVCRALSYEGLENGLNIVAINAGT
                     HCNPSFGGDTFYAVTEVLEKWELPQAHLGALRLRLLGLKNLHGSALPAAKVDGGGKSE
                     YHPNVVLDLDYTILMPRR"
     gene            complement(4181..5062)
                     /locus_tag="VN084_00185"
     CDS             complement(4181..5062)
                     /locus_tag="VN084_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWT27900.1"
                     /translation="MNNHLRPRRSMLYVPGCNTRYLNKARSLRVDSVILDLGDPILVE
                     AKEQSRLNVVEAVRQGGYGKREVVVRVNDFDSPWGRDDIKAVANIGADAILFPNIESR
                     EDVLVALEALDAAGGKQVPIMVMIESPIAVLNAKEIAAASDRIICMVVATSDLMSQLR
                     ARKTHERIALITSLSMVILAARAYGRAVIDGITNDLNDMQTFEYSCRLGRDLGFDGKS
                     LVHPSQLDYSNDAYTPRPTEIKHAQAIIEALAAANAAGRGTVVVDGKLVEHHHVAAAQ
                     QLLDMSAMIAKLEEGFE"
     gene            complement(5308..>6254)
                     /locus_tag="VN084_00190"
     CDS             complement(5308..>6254)
                     /locus_tag="VN084_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWT27901.1"
                     /translation="ARFWRMIEKHRVTVFYTAPTAIRSLIKASEANPDAHPERFDLSS
                     LRLLGSVGEPINPAAWRWYYEHVGGSRCPILDTFWQTETGGHVITPLPVTPLVPGSCT
                     LPLPGIQAAVVDESGQEVEWGTGGFLVIKRPWPSMIRTIWHDDERYKASYFPEDLGGK
                     LYLAGDGAIRDKTTGYFTIMGRIDDVLNVSGHRMGTMEIESALVANPLVAEAAVVGKP
                     HDIKGEAIVAYVVLKGIRPQGEDAKKIVANLREWVGKEIGPIAKPDDIRFGDNLPKTR
                     SGKIMRRLLRTLAKGETITADVSTLENPAILDQFKQPL"
BASE COUNT         1078 a         1805 c         2064 g         1307 t
ORIGIN      
        1 ataaaaatcc cgcctgccaa atggcagaaa aatgcaggcg ggggttaagc cggctattga
       61 cgttgtgtta tcggtacata ggcacgatct tccggccctg tgtattccgc gttggggcgg
      121 atgatcttgt tgtcgacacg ctgctcgatg acatgcgcga cccagccggt ggtgcggctg
      181 atgacgaaga tcggtgtgaa catcccggtg gggatgccca tcatgtgata gctcgacgcg
      241 ctgtaccagt cgaggttggg gaacatcttc ttctcgcgcc acatcacggt ctcgaggcgg
      301 tcggagatgt cgaacagatg catgtcgccg gcatcctggc acaggctgcg cgagacttcc
      361 ttgatggcga cgttgcgcgg atcggcgatg gtatagaccg ggtggccgaa gccgatgacg
      421 atctccttgt tggcgatccg gcgcacgatg tctgcttcgg cttcgtccgg tgtcttgtag
      481 cgttcgatga tctccatcgc cgcttcgttg gcgccgccat gcttgggccc gcgcagggca
      541 ccgatggcac ccgtgatgca ggagtacata tcggatagcg tgccggcgat cacccgtgcg
      601 gtgaaggtcg aggcgttgaa ctcgtgttcg gcatacagca cgagcgaggt gttcatggcc
      661 ttgatatgca gctcggatgg cttcttgcca tgcagcacat gcaggaagtg cgcggcgatg
      721 gaatcgtcat cggtgacgac ctcgacgcgt ttgccgttgt ggctgtagtg gtaccaatac
      781 agcaggatgg agccgaagca ggagatgagc cggtcgccca gatcctttga accctggata
      841 ttgcggtcgg ccgcttccgg ttccagcgtg cctagcatgg cacagccggt gcgcatgacg
      901 tccatcggat gcgccgatgc ggggatctgc tccagcacct tcttcaacgc ctcgggcagt
      961 gcgcgcagct gcttgagctt cttgtggtag tccgccagtt gctgccggtt gggcagctcg
     1021 ccgtgaatca acaggtaggc cacctcttcg aaagtggcgt gcttcgccag ctccaggatg
     1081 tcatagccgc gataatgcag gtcgttgccg ctatggccga cggtgcagat ggcggtggtt
     1141 ccggcggcga cgccggcaag cgccacgcct ttcttgcgtt tgggttcaac gactgcgttc
     1201 atgccttatt cctccgtgcc ttgctggctc gcgaacagct ggtccagctt ctcttcgtat
     1261 gcgtggtaat ccaggtattt gtagaggtcc atgcgcgtct gcatggtgtc gaccacgttc
     1321 ttttgcgtgc cgtcacgacg cacggcctga tagaccttga gcgcggcggc gttcatggcg
     1381 cggaacgccg acagcggata cagcaccatg ccgacgttga cgctcgccag ctcctgtacc
     1441 gtgaatagcg gcgtcgaacc gaactcggtg atgttggcca gcaccggtac cttcaccgcc
     1501 tcggcgaatt gcttgtacat ctgcaagctg gtgatggcct cggggaagat catgtcggcc
     1561 ccggcttcga cacaggcgca ggcgcggtcg atggcggatt gcaggccttc cacggcaagc
     1621 gcgtcggtgc gggccatgat gacgaagtcg gggtcggtct tggcgtcgac ggcggccttg
     1681 atgcggtcga ccatctcgac ctggctgacg atggccttgt tcgggcgatg gccgcagcgc
     1741 ttgctctgta cctggtcttc gatatgcacg gccgcggcgc cggccttggt catggccttg
     1801 atggtgcgcg cgatgttgaa ggcgccgccg aagccggtat cgatatcgac cagcagtggg
     1861 gtctcgacgt tgtcggtgat gcggcgcacg tcgatcagca cgtcctctag cgtcgagatg
     1921 ccgagatccg gcaagccgag cgagccggct gcgacaccgc cgccggaaag gtagatggcc
     1981 ttgtagccgg tctgcgtcgc caggcgcgca tggtaggcgt tgatggcgcc gatgatctgt
     2041 agcggcttct cggcggcgag cgcggcgcgg aaacgggcac cggcggaatg gagctgactc
     2101 atggtgatcc tttaagcttg gccgttaagc gaaaaaagcg tgggtcgcgt tctgcgggat
     2161 acccaccccg gtgatgcggg ccttttgcag cacttcccag aagtagcggt aggtcgcacg
     2221 gtcgtgcagt tccccggcat actggatcgg cccccattcg gcctgctggg cagccagcag
     2281 gatattggcg gcatcctcga cctcgctgta gtcgggcttc atggcgtcga cgatggcctg
     2341 gatctgcgtc gggtagatgc tccacatgcg catgaaaccg aactcgttgc gcgcgcggct
     2401 ggcatcgctg taggtggtct ccgtgttctt caggtccagc gtgacgttat gcgccggcac
     2461 cacgccgtgc gccagtgcag cggcgacgac atcagccttg gcgcgggcca gcagacgatg
     2521 ctcgaactgt ccgggactgc gcatcgcgct cgcggggatg gcgccgtgat ggccgctgac
     2581 gaaatccatg aggccgaaat ccagcacctg cagccagggc agggcagcga tctcataaac
     2641 ttcatgcagc gcgccgtggg tctcgatcag cacatgcacc gggatctcgc gctggatgcc
     2701 gtgggaagtg gcgaccttct gcaggtaggc gatcatctcg gcagcctggg cagcgcgcgt
     2761 gcacttgggg atggtgatgt agctcagcac ctggcctgcg ccaccaacca ggatgtcgat
     2821 atcctgcttc cacgcagggt gggtgtagtc gtggatgcgt gcgccggcca tgcggtgctt
     2881 gttggcgtct gaattgagca cgcgcacgat catctcggcg tgttcgcgtt cctggcccga
     2941 ggcggcgccg tcttcgcaat cgcaggtgat atcgaagatc ggccccaggc tatcctgcag
     3001 cgacagcgcc ttgaggatga gcttttcgct accggcgaaa tgttcgcacg aggggatcac
     3061 cgggaacggc ttctcgccgg cgaacaaggc ttcgttggga tggactgaca tgcgcgttat
     3121 tcctatctgc gaggcatcaa aatggtgtag tcgagatcga gcacgacatt cgggtgatat
     3181 tcgctcttgc cgccgccgtc gactttggcc gcaggcaggg cggagccgtg caggttcttc
     3241 aggccgagca ggcgcaggcg caatgcacca aggtgcgctt gcggcaattc ccacttctcc
     3301 agcacttcgg tcacggcata gaaggtatcg cccccgaagc ttggattgca gtgcgtgccg
     3361 gcgttgatgg cgacgatgtt gaggccgttc tccagccctt cgtaactcag cgcacggcaa
     3421 accgagatca cgtggccgcc gtacatgagg cgctgcccga acgccgaatc ctgcatcatc
     3481 aaggcgtcga agtgcaggcg cgcgttgttc tggtagagcc tggtcgccag cgtgtggtcg
     3541 ctgtcgtcca cggtcatgcc agccgggtgg tcgatgcgct cgcctgcctg gtaatcgtcc
     3601 cacaggcgcg tgctgccggt ggcgtgcgac gccagttgcg tcggctgcag gaagcgcggg
     3661 atggcgagct gcttcacgcc gaccacggcc ggcagttccg ggatcacggt ttccggtgct
     3721 ggcgcatcct ggtcgcgctt atgcaccatg acccagcgca cccagctcaa taccacttca
     3781 ctgtgctggt tgcggctttc ggatcgcaca tagacgaccc cggacttgcc gttggaattc
     3841 tgcttgaggc cgatgacggt cgaggtcgtg aacagggtgt cgccgacata gaccggccgc
     3901 aggaagcgca catcggcata gccgagattg gccacggcgt tgaccgagat gtcgggcacg
     3961 gtcttggcga aggcgatatg gaacgccagc agatcatcca ctggcttgtc gcgatagccg
     4021 agcgcctggg ccacgggcgt cgcgcaatgc aggggctggc gcgcgccggt gagggcgata
     4081 tagagcgcgc actcgccttc ggtgatggtg cgtggcgtgg cgtgatggat ctcctgcccg
     4141 agccggaagt gttcgaagaa attgccgctg gcggtcgtca tcattcgaac ccttcttcca
     4201 gcttggcgat catcgcgctc atgtccagca gctgctgtgc ggcggcgaca tggtgatgct
     4261 ccaccagttt gccgtcgacc accaccgtac cgcgcccggc ggcgttggcg gcagccagtg
     4321 cctcgatgat ggcctgcgcg tgcttgatct cggtcgggcg tggcgtgtaa gcgtcgttgc
     4381 tgtaatccag ctgcgacgga tgcaccagcg acttgccgtc gaacccgagg tcgcggccca
     4441 agcggcagga gtattcgaag gtctgcatgt cgttaagatc gttggtgata ccgtcgatca
     4501 cggcgcgacc gtaggcgcgt gccgccagga tcaccatcga gaggctggtg atgagggcga
     4561 tacgttcatg cgtcttgcgc gcccgcagtt gcgacatgag gtcggaggtg gcgaccacca
     4621 tgcagatgat gcgatccgac gcggccgcga tctccttggc attgagcacc gcgatggggc
     4681 tctcgatcat gaccatgata ggcacctgct tgccacccgc agcgtcgagc gcttcgagcg
     4741 cgaccagcac atcctcacgt gactcgatgt tggggaagag gatggcgtcg gcgccgatat
     4801 tggcgaccgc cttgatgtcg tcgcgacccc atggcgaatc gaaatcgttg acgcggacga
     4861 ccacttcacg cttgccgtaa ccgccttgcc tgacggcttc gacgacattc aggcgcgact
     4921 gctccttggc ttcgaccagg atggggtcgc caagatcgag aatgacggaa tccacgcgca
     4981 atgagcgcgc cttgttcagg tagcgtgtgt tgcagcctgg cacgtacagc atggagcggc
     5041 gtggccttag atgattgttc atacgtaaac cctgtgttga tcagccgttt gaatctgcat
     5101 atatagctga tatggcgttg gttttgtcat ttttattgat gcgttgcact gattctatcg
     5161 gctgcgcttt cgaagtgtca ataaatatct tatgtcttat ataagatata aaactaagat
     5221 ggccgctatt cgacgtgatc cttttggatt caatgccata tcggcgcgca gacgttgcgc
     5281 cgatacaaca tcgcatcgcg taccagctca aaggggttgc ttgaactgat cgaggatcgc
     5341 cgggttctcc aatgtcgata catcggccgt gatcgtttcg cccttggcca gcgtgcgcaa
     5401 caggcgccgc atgatcttgc ctgagcgggt cttgggcagg ttgtcgccga agcggatgtc
     5461 gtcaggtttt gcgatcgggc cgatctcctt gcccacccat tcgcgcaggt tggcgactat
     5521 ctttttcgcg tcctcgccct gtggccgtat gcccttgagc acgacatagg cgacgatggc
     5581 ttcacccttg atgtcgtgcg gcttgccgac cacggcagcc tctgccacca gcgggttggc
     5641 gaccagggcc gattcgatct ccatggtgcc catgcggtga ccggagacgt tcaatacgtc
     5701 gtcgatgcgg cccatgatgg tgaagtaacc tgtggtcttg tcacggatgg cgccatcgcc
     5761 ggcgagatac agtttgccgc cgaggtcctc tgggaaatac gatgccttgt agcgttcgtc
     5821 gtcgtgccag atggtgcgga tcatcgaagg ccaggggcgt ttgatgacca ggaacccgcc
     5881 cgtaccccat tcgacttcct ggcccgattc gtcgacgacg gcagcctgga tgccagggag
     5941 tggcagggtg caggagccgg gcaccagcgg tgtgacgggt aatggggtga tcacgtggcc
     6001 gccggtttca gtctgccaga aggtgtcgag gatagggcag cgcgagccgc cgacgtgctc
     6061 gtagtaccag cgccaggcgg ccgggttgat cggctcgccg acagagccca gcaagcgcag
     6121 ggagctgagg tcgaagcgct cgggatgggc gtccgggttc gcttccgaag ccttgatcag
     6181 cgaacggatg gcggtcggcg cggtgtagaa gacggtcacg cgatgttttt cgatcatgcg
     6241 ccagaagcgc gctg
//
LOCUS       DATMTW010000011         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_537505, whole genome shotgun sequence.
ACCESSION   DATMTW010000011 DATMTW010000000
VERSION     DATMTW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537505"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VN084_00195"
     CDS             complement(<1..193)
                     /locus_tag="VN084_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HWT27902.1"
                     /translation="MLARLLLVLSLLTIFTQVQAATVITAARVWPSAEYTRITLESSK
                     PITHRMVMLKDPDRLVLDLD"
     gene            complement(187..651)
                     /gene="tsaE"
                     /locus_tag="VN084_00200"
     CDS             complement(187..651)
                     /gene="tsaE"
                     /locus_tag="VN084_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWT27903.1"
                     /translation="MAHDITLHLSDEAATLSFGGRLAKCMRPGLSIYLHGDLGAGKTT
                     LVRGLLHGLGHTGKVKSPTYTLVEPYVVSGLDLYHFDLYRFNDPEEWEAAGFRDHFNP
                     DTICLVEWPEKARALLPAPDIDITLTPAAGGRQVTLHAPTAAGAQTLEALAC"
     gene            643..1731
                     /gene="queG"
                     /locus_tag="VN084_00205"
     CDS             643..1731
                     /gene="queG"
                     /locus_tag="VN084_00205"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWT27904.1"
                     /translation="MRHMSESPIDWNALAAEIKRWGLELGFGQIGITDTDLTDAESAH
                     RAWVAKGFHGAMDYMAKHGDKRTRPAELVSGTLRVISARMDYMPPAARDSMEVMAQGD
                     LAFISRYALGRDYHKVLRSRLQKLCDRISHTAGGFGYRVFTDSAPVLEVALAEKAGLG
                     WRGKHTLLLSRDAGSWFFLGEIYTDLPLPTDTPTQNHCGTCKACIDICPTQAIVAPYE
                     VDARRCISYLTIELKDAIPQALRPLIGNRVYGCDDCQITCPWNRFAQAAQEPDFHVRH
                     GLDDISLVELFGWDEAMFNQRMAGSAIYRIGYLQWLRNLAVGLGNAPTSPAVIEALQG
                     RAQHPSPMLREHVAWALQQHAHAHGASA"
     gene            1777..>2346
                     /locus_tag="VN084_00210"
     CDS             1777..>2346
                     /locus_tag="VN084_00210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWT27905.1"
                     /translation="MANRSLLQKASTALFVLLGLFILLTFRQYGISNDEEVQHVYGRL
                     LLDFYASGFSDQAAFSYRNLYLYGGFFDLIAASLERLLHLAPDSIWLWDMRHLLSALF
                     GFIGIVAVFKTARLLGGLRAAFLAALLLSITGAWSGAMFTHTKDVPFGACMAWALYYT
                     VLISQQLPRFPRALAIKLGIAVGCALGLRI"
BASE COUNT          441 a          722 c          689 g          494 t
ORIGIN      
        1 catcgagatc cagtaccagc cggtccgggt ctttcagcat gaccatgcgg tgggtgatcg
       61 gcttagacga ttcgagggtg atgcgtgtgt attcggccga tggccagacc cgcgccgccg
      121 tgatgacggt ggcggcctgt acctgggtga agatggtgag caggctcagg acgagcaaga
      181 ggcgagctag catgcgagcg cctccagggt ttgcgcgcct gcggctgtcg gcgcatgcag
      241 cgtgacttgt ctgccaccgg cagcgggcgt cagggtgata tcgatatccg gcgccggcaa
      301 caaggcgcgg gctttttccg gccactccac gaggcaaatg gtgtcgggat tgaagtgatc
      361 gcgaaagccg gctgcttccc attcttccgg gtcattgaat ctatataaat caaagtgata
      421 caagtctaat ccagaaacga catacggttc aaccagggta taggtcgggc ttttgacttt
      481 gcccgtgtgc cctagcccgt gcagcaaacc acgcaccaac gtggtcttgc ccgcgccaag
      541 atcgccatgc aggtagatgg atagtccggg ccgcatgcac tttgccaggc gacctccgaa
      601 ggacagggtc gccgcttcat cgctcaggtg caatgtaata tcatgcgcca tatgtcagag
      661 agcccgatcg attggaacgc cctcgccgcg gagatcaaac gctggggcct ggaacttggt
      721 tttggccaga tcggtatcac cgatacggac ctgactgacg ctgaaagtgc acatcgcgcg
      781 tgggtggcta aaggctttca tggtgccatg gattatatgg caaaacatgg cgacaaacgt
      841 acccggccgg cagaattggt ctcaggcacc ctgcgcgtga tctccgcccg aatggactac
      901 atgccgcctg ccgcaaggga tagcatggaa gtcatggcgc aaggtgacct tgccttcatt
      961 tcgcgctatg cgctgggacg cgactatcac aaggtgctgc gtagccgcct gcagaagctc
     1021 tgtgacagga tctcgcacac agctggtgga ttcggctacc gcgtgttcac cgacagcgcg
     1081 ccagtactgg aagtcgcgct ggcagaaaag gccggcctgg gctggcgagg caaacatacc
     1141 ttgctgctat cccgcgatgc cggctcctgg ttcttcctcg gcgagatcta taccgacctg
     1201 cccttgccga ccgatacgcc gacccagaac cattgcggca cctgcaaggc ctgcatcgat
     1261 atctgcccga cacaggccat cgtcgcgcct tacgaggtgg atgcccgacg ctgcatctct
     1321 tacctcacca tcgaactcaa ggacgcgatc ccgcaagcac tgcggccgtt gatcggcaac
     1381 cgtgtctatg gttgtgacga ctgccagatc acctgcccct ggaatcgctt cgcgcaagcc
     1441 gcacaagagc cggatttcca tgtgcggcac ggactggacg atatcagcct ggtcgaactg
     1501 ttcggctggg acgaagccat gttcaaccag cgcatggccg ggagcgccat ctaccggatc
     1561 ggctacctgc aatggctgcg caacctcgct gtggggctcg gcaatgcgcc gacatcgcct
     1621 gccgtgatcg aggcgctgca aggccgcgcg cagcacccgt cgcccatgct gcgcgaacat
     1681 gtcgcctggg ccttgcagca acacgcgcac gcgcatggag caagtgcctg attatcgcta
     1741 taatccggcc ttcttcattg cgtccagcgt gccatcttgg ctaatcgatc cctgctgcag
     1801 aaagcttcca ccgccctgtt cgttctgctg ggcttgttca tccttcttac tttcaggcag
     1861 tacggcatca gcaatgatga agaagtgcag catgtctacg gaaggctgct gctcgatttc
     1921 tatgcctccg gcttcagcga ccaggcggcg ttctcttacc gaaatctcta tctttacggc
     1981 ggctttttcg atctgatcgc cgcctcgctg gaacgcctgc tgcacctggc gccggattcc
     2041 atctggctat gggacatgcg ccacctgctg tcggccttgt tcggtttcat cggtatcgtc
     2101 gccgtattca agacggcacg actgcttggc gggctgcgcg cggcctttct cgcggcttta
     2161 ctgctgagca tcaccggtgc ctggtcaggc gccatgttca cgcacaccaa ggacgttccc
     2221 ttcggcgcat gcatggcctg ggccctctac tacaccgtgc tgatctcgca acagctgccg
     2281 cgatttccgc gtgcgctcgc gatcaagctc ggcatcgctg tcggttgtgc gctaggcctg
     2341 cgcatc
//
LOCUS       DATMTW010000012         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_369782, whole genome shotgun sequence.
ACCESSION   DATMTW010000012 DATMTW010000000
VERSION     DATMTW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369782"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="ychF"
                     /locus_tag="VN084_00215"
     CDS             complement(<1..593)
                     /gene="ychF"
                     /locus_tag="VN084_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWT27906.1"
                     /translation="MSMKCGIVGLPNVGKSTLFNAITRAGIAAENYPFCTIEPNVGIV
                     EVPDPRLKPLIDIVNPQKVQPAIVEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAH
                     VVRCFDDGNVVHVAGKVDPLSDIEVINTELALADMETVEKTLAREGKKAKSGDKEALA
                     LCAVLEKVQKHLDAGHQVRTLGLDADQLAIIKPLCLIT"
     gene            complement(713..2422)
                     /locus_tag="VN084_00220"
     CDS             complement(713..2422)
                     /locus_tag="VN084_00220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT27907.1"
                     /translation="MLAIFSHHRVKIYFLFTFLLTATAFGLSRIDWESSVAVHTLMEA
                     MATLLAFTVGALSLIRYYSHAENKYLLVGLGFIGAAILDGIHGTATSIWFHERFPSEL
                     QRLLPWSWFASRLFLSVMLLLSIVPWLYGKPQHRESETNPARLYFMAIFAICACLLLI
                     SFVHLPPAYYAHLPAARPEEALPAACFALALIGYLRQGQWRSVAVDHWMVLALIASLF
                     CQAVFMPFSSHLFDTNFDVAHILKQLSYLFVLIGLLISTYQSFRQLAHEIAEHRAADI
                     RNRMLVAAIEKSPVAVMVTNAMAKIDYCNRKALEMLGYESAEIIGRDPRMFSSGKTPK
                     HTYDAMWARLKHGEDWNGIFFNRKKSGEELIDESHISPIMDEQRNITHYVAIKLDATE
                     RIIKERDTWHKANYDRLTDLPNRGLFLDRLDNALRNAKRVETNLALLFIDLDGFKAVN
                     DTYGHQAGDHLLQWVASQISRVIRDNDTISRFGGDEFALLVADFTVDAEVRVVAEKVL
                     DAISLPCPLGAQAISISASIGVALYPEHGNTADQLITRADKAMYHVKKQSGRGIAFHT
                     VEV"
     gene            complement(2506..3084)
                     /gene="pth"
                     /locus_tag="VN084_00225"
     CDS             complement(2506..3084)
                     /gene="pth"
                     /locus_tag="VN084_00225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWT27908.1"
                     /translation="MHGIQLIVGLGNPGPQYEATRHNAGFWWVDQIASEHGSRLNTES
                     KFQGVAGRLKQGDHEAWLLKPSTFMNASGRAVAAIANFYKIPPQAILVVHDELDLGPG
                     IVKLKKGGGHGGHNGLKDITAALGTADFWRLRLGIGHPGERNEVVNFVLRPPSRDEMH
                     AIESAMDNSANLLPMLLKGEFEAAMLKLHTKA"
     gene            complement(3168..3809)
                     /locus_tag="VN084_00230"
     CDS             complement(3168..3809)
                     /locus_tag="VN084_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWT27909.1"
                     /translation="MEIIIQASKREGKGTGASRRLRRAGSVPGVIYGAGKDAVSVQFD
                     HKTLFLQFRHEAFHASILTLDLEGKKESVLLRDYQMHPVRNTIQHIDFQRVSATEKIH
                     VKVPLHFVNEEVGPGVKLGGGIVTHILTEADVSCLAKDLPEFIEVDLGNAEVGQTIHL
                     SEIKIAKGIEFVQLAHGNDAAVATITKPRGAATDEETTAEAVEGEAPAAPAAE"
     gene            complement(3957..4892)
                     /locus_tag="VN084_00235"
     CDS             complement(3957..4892)
                     /locus_tag="VN084_00235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWT27910.1"
                     /translation="MMVFTGTANPKLAEEVVSHLGISLGRATVDRFSDGEVMVELMDN
                     VRGKDVFVLQSTCMPTNDNLMEVMVMVDALRRSSAGRITAAIPYFGYSRQDRRPRSAR
                     VAITAKVVANMLTSVGVNRVLTMDLHSDQIQGFFDIPVDNIYATPILLSDLWKRNYDD
                     LVVVSPDVGGVVRARACAKQLNSDLAIIDKRRPKPNVAKVMNIIGDVAGRTCVIMDDM
                     VDTANTLCEAASALKEQGAVKVVAYCTHPVLSGSAIERISASDLDELVVTNTIPLRAD
                     AAGCSKIRQLSTAELMAETMRRISNEDSVSSLFME"
     gene            complement(4968..5044)
                     /locus_tag="VN084_00240"
     tRNA            complement(4968..5044)
                     /locus_tag="VN084_00240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5008..5010),aa:Gln,seq:ttg)
BASE COUNT         1027 a         1397 c         1645 g         1129 t
ORIGIN      
        1 gtgataaggc atagcggctt gatgatggcg agctggtcgg catccagccc cagcgtgcgg
       61 acttggtggc cagcgtcgag atgcttctgt actttttcca gcacggcgca tagggccagt
      121 gcttccttgt cgccggattt ggctttcttg ccttcgcgcg ccagtgtctt ttcgacggtt
      181 tccatgtcgg ccagcgcgag ttcagtattg atgacctcga tatcggaaag cgggtcgacc
      241 ttgccagcga cgtggacgac gttaccgtca tcgaaacaac gcacgacgtg agcgatggca
      301 tcggtctcgc ggatgttggc gaggaacttg ttgcccaggc cttcgccctt ggaggcgccg
      361 gcgaccaggc cggcgatatc gacgaactcg acgatggcag gctgcacctt ctgcggattg
      421 acgatatcga tcaagggctt gaggcgcgga tcggggactt cgacgatgcc gacgttgggc
      481 tcgatggtac agaagggata gttctctgcc gcgatgcctg cacgcgtgat ggcgttgaac
      541 agggtggatt tacccacgtt aggcaaaccg acgattccgc atttcatgga gatgagacct
      601 ttcttggcag caggcaggct gctgtggctg gctgtggtac tggatcagcg cgccgggagt
      661 gattgaataa aggcgctatt ttacctgtaa gccgccggtg cacaaaggtc gattaaacct
      721 cgacggtatg gaaagcgatg ccgcgtccgg actgtttctt gacgtgatac atggctttat
      781 ccgcacgggt gatgagctgg tcggcggtat tgccgtgctc ggggtagagt gcgacgccga
      841 tggaggcgga gatgctgatc gcctgtgcgc ctagcggaca gggcaggctg atggcgtcga
      901 gtaccttttc ggccacgacg cgcacttcgg catcgacagt gaaatccgcc accagcagcg
      961 cgaattcgtc gccgccaaaa cggctgatgg tgtcattgtc acggatgacg cgactgatct
     1021 gcgaggcgac ccactgcagt agatgatcgc ccgcctgatg gccgtaggtg tcgttgaccg
     1081 ccttgaagcc gtcaaggtcg atgaatagca atgcgaggtt ggtctccacg cgcttggcgt
     1141 tgcgcagggc gttgtccagg cgatcgagga acaggccgcg gttgggtagg tcggtcaggc
     1201 ggtcgtaatt ggccttgtgc caggtatcgc gctccttgat gatgcgttcg gtggcatcga
     1261 gcttgatcgc gacgtaatgc gtgatgttgc gttgttcgtc catgatgggc gagatgtgtg
     1321 attcgtcaat cagctcttcc ccggattttt tgcggttgaa gaagatgccg ttccagtcct
     1381 ctccatgctt cagccttgcc cacatcgcgt cgtaggtatg tttcggcgtt ttgccggagg
     1441 agaacatgcg cggatcgcga ccgatgatct cggcactttc gtaacccagc atctcaagcg
     1501 ccttgcggtt gcaataatcg atcttggcca tcgcattggt caccatgacg gcaaccgggc
     1561 ttttctcgat ggcggcaacg agcatgcggt tgcggatatc ggcagcgcgg tgttccgcga
     1621 tctcgtgcgc gagttgccgg aacgattgat aggtgctgat caggagaccg atgagtacga
     1681 acaggtagct cagctgcttg aggatatgcg cgacgtcgaa attggtatcg aacaggtggc
     1741 tagagaacgg catgaacacc gcctggcaga acaggcttgc gatcagggcc agcaccatcc
     1801 agtgatcgac ggcgaccgat cgccattgac cttggcgcag ataaccgatg agcgccaggg
     1861 cgaagcatgc ggcgggcaag gcttcttccg gccgggcggc aggcaggtgc gcatagtagg
     1921 cgggcggcag atgcacgaac gagatcagca gcaggcaagc gcagatggcg aatatggcca
     1981 tgaagtagag ccgggcgggg ttggtttcgg attcccgatg ttgcggtttg ccatagagcc
     2041 agggcacgat gctgagcagc aacatgacgg aaaggaacag acgtgaggcg aaccagctcc
     2101 aaggcagcaa gcgctgcagc tcggagggaa agcgctcatg gaaccagatc gaggtggcgg
     2161 tgccgtggat gccatcgagg atagccgcgc cgatgaagcc aaggccgacc agcagatatt
     2221 tgttttcggc gtgactgtag tagcggatga gcgataatgc cccgaccgta aaggcaagca
     2281 gcgtcgccat cgcttccatc agcgtatgca ctgcgaccga gctttcccag tctatgcggg
     2341 ataagccgaa tgccgttgca gtcagcaaga acgtgaacag gaagtagatc ttgacgcgat
     2401 gatggctgaa aatggccagc aaaatcgtat ccccgattaa gtacgattca atttacaccc
     2461 aatatgggtg caagtcatca agcaataatc aggctaggtg ctggctcagg ccttggtatg
     2521 caacttgagc atggcggctt cgaactcacc cttgagcagc atgggcagca gattcgcgct
     2581 gttatccatc gcgctttcga tggcgtgcat ctcgtcgcgc gaaggcggcc tgagcacgaa
     2641 gttgacgacc tcgttacgtt cgcccggatg tccgatgccg aggcgcaggc gccagaaatc
     2701 cgctgtgccg agcgctgcgg tgatgtcctt gagtccgtta tgcccgccat gcccgccgcc
     2761 cttcttgagc ttgacgatgc cggggccgag gtcgagttca tcatgcacga ccaggatggc
     2821 ctgtggcggg atcttgtaga aattggcgat ggcggcgacc gcgcgaccgc tggcgttcat
     2881 gaaggttgaa ggcttgagca gccaggcttc gtggtcgccc tgtttgagac gtccggcgac
     2941 cccctggaac ttgctctcgg tattgagcct gctgccgtgt tcactggcga tctggtccac
     3001 ccaccagaag ccggcgttgt gacgcgtggc ttcgtattgg gggcctgggt tgccgaggcc
     3061 gacaatgagc tggataccgt gcatcagtgg tttaccttat ccctgaaaat aaaaacgcgc
     3121 gctccagtct cccggaacgc gcgtcatttt acaacgccag gctggaatta ctcagcagct
     3181 ggtgcggctg gtgcttcgcc ttcgacagct tcagccgtgg tttcttcatc ggttgctgcg
     3241 ccacgtggct tggtgatggt cgcgactgca gcgtcattgc catgcgccag ttgtacgaac
     3301 tcgatgccct tggcgatctt gatctcggac aagtggatgg tctggccgac ttctgcattg
     3361 cccagatcga cttcgatgaa ctctggcagg tctttcgcca ggcagctgac atcggcttcg
     3421 gtcaggatgt gggttacgat accgccgccc agcttcacgc cagggccgac ttcttcgttg
     3481 acgaagtgca gtgggacctt gacatggatc ttctcggtgg cgctcacgcg ctggaagtcg
     3541 atgtgctgga tggtgttgcg gacggggtgc atctggtagt cacgcagcag gacggattcc
     3601 ttcttgcctt cgaggtctag ggtcaggatg gatgcgtgga acgcttcgtg gcggaactgc
     3661 aggaacaggg tcttgtggtc gaactgcacg ctgactgcgt ccttgccggc accatagatg
     3721 acgcccggaa cactgccggc gcgacggaga cggcggctcg cacccgtacc tttgccttcg
     3781 cgcttgcttg cttgaatgat gatttccatt ttactactcc ataaagtgct gaaacggctt
     3841 ggcgggcacc ttgttggcgc atcgcttcag tcattccagc tttctaaaac tgttatccgc
     3901 gaccagataa caggcttgtg atccggctgc tcgcgcaacc cgatcgaaac ttttcgctat
     3961 tccatgaaca gcgagctgac cgaatcctcg ttgctgatgc ggcgcatggt ctcggccatg
     4021 agttcggcgg tactcagctg gcggatcttg ctgcagccgg cggcatcggc acgcaacggg
     4081 atggtgttgg tgacgaccag ttcatcgagg tcggaggccg agatgcgttc gatggccgag
     4141 ccggacagca ccgggtgcgt acagtaagcg acgactttca ccgcaccttg ctctttcagc
     4201 gcggaggcgg cttcgcacag cgtattcgcg gtatcgacca tgtcatccat gatgacgcag
     4261 gtgcggccgg cgacatcgcc gatgatgttc atgaccttgg cgacgttcgg cttcggacga
     4321 cgtttgtcga tgatggccaa gtcggaattc aattgttttg cacaggcacg ggcgcgaacc
     4381 acgccgccga catcgggcga tacgacgacc aggtcatcgt agttacgctt ccacaggtcg
     4441 ctcagcagga tcggtgtcgc gtagatgttg tcgaccggga tgtcgaagaa cccctggatc
     4501 tggtcggagt gcaggtccat ggtcaggacg cgattgacgc cgacgctagt cagcatgttg
     4561 gcgaccacct tggcggtgat ggcgacacgg gcagagcggg ggcggcgatc ctgacgcgaa
     4621 taaccgaaat aggggatggc ggcggtgatg cggccagcgg aagagcgtcg cagcgcatcg
     4681 accatgacca tgacttccat caggttgtca ttggtcggca tgcaggtgga ctggagcacg
     4741 aagacatcct tgccgcgcac attgtccatc agctcgacca tgacttcacc gtcgctgaaa
     4801 cggtcgaccg tggcgcggcc gaggctgatg cccaagtggc tgacgacttc ctcagcaagc
     4861 ttggggttgg cagtgccagt gaacaccatc atgtcgccat tagccacagc gaagtccctt
     4921 atcgtcaaaa aaataaaagc gtgtaaggct acacgcttaa cttgttctgg ctggggagga
     4981 aggattcgaa ccttcgcatg ccggaatcaa aatccggtgc cttaaccagc ttggcgactc
     5041 cccaattgtg tgttgcgatt gcttcagtcc gcgaagtcat agagtgggtg ctgttccaga
     5101 cccttggcga gaaatccgac atattgtttc ggcaactcgg catatctcga ctccgcttcg
     5161 gcttgttgat cgaactctac gaacacagac gcaccgga
//
LOCUS       DATMTW010000013         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_101539, whole genome shotgun sequence.
ACCESSION   DATMTW010000013 DATMTW010000000
VERSION     DATMTW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101539"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /gene="rimI"
                     /locus_tag="VN084_00245"
     CDS             <1..268
                     /gene="rimI"
                     /locus_tag="VN084_00245"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWT27911.1"
                     /translation="LVLDEAHLLNLSIAHTHQGRGLGRALLEYMMQISRGHGAANIFL
                     EVRPSNTPAIRLYEQVGFSEMAVRRGYYPAAHGREDAILMGMAL"
     gene            279..1280
                     /locus_tag="VN084_00250"
     CDS             279..1280
                     /locus_tag="VN084_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWT27912.1"
                     /translation="MSLTREDMLRELELLPVWKLREQPQATDTAPQPHPSAEAQAVAT
                     TSLPPQPIEMQATVVPSPAVPSSAEMPIVENPMVTQPLAQAVPLGMPAMVAEQPDAPW
                     DETPVSDDSLAVEEALPPLTTSPGEDRRERIMKLDWAGLQACVPDCQACGLAGTRTQT
                     VFGVGDPQAEWLIVGEAPGAEEDKRGEPFVGQAGKLLDNMLAAIKLKRGENVYIANVL
                     KCRPPENRDPHGEEVAQCDPFLKRQVELIQPKLILALGKFAAQSLLGSDDTIGAMRGK
                     LHRYNGVPVIVTYHPAYLLRNLMDKAKAWEDLCFAVKTMQTLQAEALQSATQPPPSH"
     gene            1309..1902
                     /locus_tag="VN084_00255"
     CDS             1309..1902
                     /locus_tag="VN084_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWT27913.1"
                     /translation="MRSLFLKLSVLLMVLTLSGCGYNTFQSLDEEVKSNWSEVLNQYQ
                     RRADLVPNLVNTVKGYASHEKEVLTAVTEARAKVSSIQATPELLNDEAAFARFQQAQS
                     QMTSALSRLLVVAENYPQLKADTSFLNLQAELSGTENRVAVARNRYIKAVKEYNIAVR
                     TFPNNLTAMMFGYKPKPNFTVDNEAELAKPPVVDFNK"
     gene            1963..2760
                     /locus_tag="VN084_00260"
     CDS             1963..2760
                     /locus_tag="VN084_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HWT27914.1"
                     /translation="MAFAADEVAVPPLQQRVTDLTQTLGADQQAALESKLAQFEQQKG
                     SQIAILLVPTTQPEAIEQYSIRVVEAWKLGRKGVDDGILILVAKADRKVRVEVGYGLE
                     GIVPDSIASQIIRDAIQPHFKQGDFYGGLNAAVDQLIARINGEPLPAPERQAAASGGW
                     EDLLPVLLFGGLLVGGLLRAIFGSFLGGMVNGGLVGAAVWLLGGGLLIAMVVAFVAFI
                     LTMSGGLGGLNGYSGGSHYGGGGFGSGGGFSGGGGGGFGGGGASGDW"
     gene            2786..3289
                     /locus_tag="VN084_00265"
     CDS             2786..3289
                     /locus_tag="VN084_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWT27915.1"
                     /translation="MMRRLKRHIQHLLSAPWQVHRYFSRQALTNIKAAIRDSERQHAG
                     QIRFAVEVGLHPYALWQGKTPRQRAIELFARLGVWDTAHNNGVLIYLLLADHDVEIVA
                     DRGIHAHVGATQWEHVCREMESMFRRGEFEAGVLFGIERIGTLLSQHFPPEHDQANEL
                     SDAPVLL"
     gene            3246..>3760
                     /locus_tag="VN084_00270"
     CDS             3246..>3760
                     /locus_tag="VN084_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27916.1"
                     /translation="MTKPMSSVMRLSCFSLLFALLPASALADSRDLDRVLRQTPAWSG
                     FEICFGGGCAGVAKAVIDDAAWAPIAQLFVPASESAAQERERVSRAIGLLEQIVGLQT
                     GTSEDRAGTFVDIRYQHQLDCNDEAANTKTYLKLLQQAGYLQFHDIIDTKTRGFFING
                     WPHTTAAIEEKA"
BASE COUNT          747 a         1156 c         1163 g          694 t
ORIGIN      
        1 gctggtgctg gacgaagcgc acctgctcaa tctaagtatc gcccataccc atcagggccg
       61 cggtctcggc cgcgcgttac tcgaatacat gatgcagatc tcgcggggac acggtgcagc
      121 caatatcttt ctcgaggtac ggccttccaa cacgcccgcg atccggctct atgaacaggt
      181 cggcttcagt gagatggccg tgcgtcgcgg ctattacccg gcggcgcatg gacgcgagga
      241 tgccatcctg atgggcatgg cgctatgagg gcgatacgat gagcctgacc cgcgaagaca
      301 tgttgcgcga gctggagtta ttgccggtat ggaagctgcg cgagcagccc caggcgaccg
      361 acacggcgcc ccaacctcat ccatcggcag aggcgcaggc ggttgcgacg acctccttgc
      421 cccctcagcc catcgagatg caggcgacag tcgtgccatc gccagctgtg ccatcgtcag
      481 ccgagatgcc gatcgtggag aaccccatgg ttacccagcc cctcgcgcaa gcggtacccc
      541 tgggcatgcc ggcgatggtc gccgagcaac ctgacgcgcc atgggacgag acgccggtgt
      601 ctgatgactc gttagcggtc gaggaggcat tgccgccatt gaccacaagc cctggcgaag
      661 accggcgcga gcgtatcatg aaactcgatt gggcgggcct gcaagcctgc gtgcctgatt
      721 gtcaggcctg tggccttgcc ggtacgcgca cgcagaccgt attcggcgtc ggtgacccac
      781 aagccgaatg gctgatcgtc ggtgaagcgc ctggcgcgga agaggacaag cgcggcgagc
      841 ctttcgtcgg ccaggccggc aaactgctcg ataacatgct ggccgcgatc aagctcaaac
      901 gcggcgagaa cgtctacatc gccaatgtat tgaaatgccg gccacccgag aaccgagacc
      961 cgcatggcga ggaagtcgcc caatgcgatc ctttcctcaa gcgacaggtc gagctgatcc
     1021 agcccaaact gattctcgcg ctcggcaagt tcgcggcgca gtcgctactt ggcagcgacg
     1081 acaccatcgg cgccatgcgc ggcaagctgc accgttacaa tggcgtgccg gtcatcgtca
     1141 cctaccatcc ggcctatctg ctacgcaacc tcatggacaa ggccaaggcc tgggaagact
     1201 tgtgctttgc cgtcaagacc atgcagaccc tgcaggccga ggccttgcaa tcggcgacgc
     1261 agccaccacc atctcactaa agaccgaacc accacacaag gagtcgtcat gcgttcccta
     1321 tttctgaaac tatccgtcct gctcatggtg ctgacactga gcggttgcgg ttacaacacc
     1381 ttccagtcgc tggacgagga agtgaagtcc aactggtcgg aagtcctcaa ccaataccag
     1441 cgccgtgccg atctggtgcc taatctggtg aacacggtga aaggctatgc atcgcacgag
     1501 aaagaggtgc tgactgccgt gaccgaggcg cgtgccaagg tcagcagcat ccaggcaacg
     1561 cctgagctgc tcaacgacga agctgctttt gcccgcttcc agcaggcaca gagccagatg
     1621 acctccgcgc tctcacggtt gctggtggtc gccgagaact acccgcaact caaggccgat
     1681 accagcttcc ttaacttgca ggcagagctc tccggcaccg agaaccgcgt ggcagtggca
     1741 cgcaatcgtt acatcaaggc cgtgaaggag tacaacatcg ccgtgcggac cttccccaac
     1801 aacctgacgg caatgatgtt cggatacaaa ccgaagccta acttcaccgt cgacaacgaa
     1861 gccgaactcg ccaagccgcc ggtcgtggac ttcaacaagt aacaacgcat gcggtcattc
     1921 ttgctccgca gtctggcggt tggcctactg cttctggcga cgatggcgtt cgccgccgac
     1981 gaagtggcgg tgccgccgct gcagcagcga gtgaccgatc tcacccagac cctgggtgcc
     2041 gaccagcaag ctgcgcttga atccaagttg gcgcagttcg agcagcagaa gggcagccag
     2101 atcgccatcc tgctggtgcc gaccacgcag ccggaagcca tcgagcagta ttcgatcaga
     2161 gtggtggagg cctggaagct cggacgcaag ggcgtcgatg acggcatcct gatcctggtc
     2221 gccaaggctg atcgcaaggt acgtgtcgaa gtcggctacg ggttggaagg catcgtaccc
     2281 gacagcattg ccagccagat catccgcgat gccatacagc cgcatttcaa acagggtgat
     2341 ttctacggcg ggctgaatgc cgcagtggat caactgatcg ctcgcatcaa cggcgaaccg
     2401 ttgcccgcgc ctgaacgtca ggcggccgca agcggtggct gggaggatct gctgccggtc
     2461 ttgctgtttg gcggcttgtt ggtcggcggc ttgctgcgtg cgatcttcgg cagctttctc
     2521 ggaggcatgg tcaacggtgg tctggtgggc gccgctgtct ggctgctggg cggcggcctg
     2581 ctgatcgcga tggtggtggc ctttgtcgcg ttcatcctca ccatgagcgg cggtctcggc
     2641 ggactcaacg gctacagtgg cggcagtcat tatggcggag gcggctttgg tagcggcggc
     2701 ggtttctcgg gcggcggcgg tggcggtttt ggtggtggcg gggcttcggg cgactggtga
     2761 tgaagaatat gatcgaaagc gaatcatgat gcgcaggctc aaacgtcata tacagcatct
     2821 gctcagtgcg ccatggcaag tgcatcgcta tttctcacgg caggcgctga ccaatatcaa
     2881 agctgcgatc cgcgatagcg agcgccaaca cgcggggcag atccgtttcg cggtcgaggt
     2941 cggcctgcac ccttacgccc tatggcaagg caagacgcca cggcaacgcg cgatcgaatt
     3001 gttcgcccgg ctcggcgtct gggacaccgc ccacaacaat ggcgtgctga tctacttgct
     3061 gctggccgat catgatgtcg agatcgtcgc cgatcgcggt atccatgcgc atgtcggcgc
     3121 tacacagtgg gagcatgtct gccgcgagat ggaatcgatg ttccgtcgcg gcgaattcga
     3181 ggcaggcgtc ctgttcggta tcgaacgcat cggcaccttg ctcagccagc attttccgcc
     3241 cgaacatgac caagccaatg agctcagtga tgcgcctgtc ctgctttagc ctgttgttcg
     3301 cgctgctgcc ggcatccgcg ctggccgata gccgtgacct cgatcgtgtg ctgcgccaga
     3361 caccggcctg gtcgggcttc gagatctgct tcggcggtgg ctgtgccggg gttgcgaaag
     3421 cggtcatcga cgatgcagca tgggcgccga tcgcgcagct gttcgtgccc gcctcggaga
     3481 gcgcggcgca ggagcgtgag cgtgtcagcc gggccatcgg cttgcttgaa cagatcgtcg
     3541 gactgcagac cggcaccagt gaggaccggg ccggtacttt cgtcgatatc cgttaccagc
     3601 atcagctcga ttgcaacgac gaagccgcca acaccaagac ctatctgaag ctgctgcaac
     3661 aagccggcta cctgcagttc cacgacatca tcgataccaa gacgcgcggt ttctttatca
     3721 acggctggcc gcacacgacc gcggcgatcg aggaaaaggc
//
LOCUS       DATMTW010000014         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_235670, whole genome shotgun sequence.
ACCESSION   DATMTW010000014 DATMTW010000000
VERSION     DATMTW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235670"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VN084_00275"
     CDS             <1..801
                     /locus_tag="VN084_00275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT27917.1"
                     /translation="HNPNTSATDDALGDFSIVLVNYKTLELTRSALEFLRQVVDVTQV
                     PVWVVDNYSEDESTEYLRSLDWIRLIERKIDTPETGALAHGRALDMVLERVDTSYLLL
                     MHTDTFIYDATILRMMLDAIRRDPTVFAVGCIDQIDRGLLRTAWRHASRFMKHYSRKT
                     MLALGLPSRQPKPYYEIYLKSFCALWNVSLIRDHAMHFSMADRTPGYEMQDRLTALGY
                     RIVYIAPRKMFRYLDHVQSGTVAAAGTYGQGHRRTRAYQAMLKKIGQR"
     gene            810..1589
                     /locus_tag="VN084_00280"
     CDS             810..1589
                     /locus_tag="VN084_00280"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HWT27918.1"
                     /translation="MKAKQFYGSESARQSLQESGLVDFESLWTLDAGWFEAPNQRRGG
                     WSGVSRYRLKDGHYVFIKRQQNHSFRSWRYLFRAIPTFTRELSRILGFASAHIPALEL
                     VYFGQREHGKDMQSILVTWELAGFKSLDDPAFVPLRRLDRASRHALIAETAAVVRQMH
                     AYRYQHNCLYPKHIFARLNAGPSAEVRLIDLEKVKWRPARRFAMLRDLGTLYRHVEGW
                     TATDQLRFFLAYRQESGLTATSKRILRKILRGTRKKLARRA"
     gene            2411..>2920
                     /locus_tag="VN084_00285"
     CDS             2411..>2920
                     /locus_tag="VN084_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HWT27919.1"
                     /translation="MEDALRTGHPLKRGNTCTVTRAMIGDRKVVVKRYNIKHWLHGLG
                     RLFRRSRASVSWANAHRLRMHGIATAMPLALVERRFGPCRLQAYFVADYIEAPDMASW
                     MADRAIDPQDKETAAMGLARLMHRLRLLQIAHGDMKATNLHVHGVRPILIDLDSMRHV
                     RCRRSFERRH"
BASE COUNT          552 a          893 c          890 g          585 t
ORIGIN      
        1 cataacccga atacctcagc gacagatgac gctttgggcg atttcagcat cgtattggtg
       61 aattacaaga cactggagct cactaggagt gcgctcgagt ttctccggca ggtggtggac
      121 gtgacccagg tcccggtctg ggtggtcgat aactattcgg aagacgaaag tacggaatat
      181 ctgcgctcgc tcgactggat ccgtctcatc gagcgcaaga tcgatacgcc ggaaaccggg
      241 gcgctggcgc atggccgtgc cctggacatg gtgctggagc gcgtggatac ctcctatctg
      301 ctgctcatgc acaccgacac cttcatctat gacgcgacca ttctgcgcat gatgctcgat
      361 gcgatccggc gtgacccgac cgtattcgcc gtcggctgca tcgaccagat cgaccgtggg
      421 ctgttgcgca ccgcctggcg tcacgccagt cgcttcatga agcattattc tcgcaagacc
      481 atgctggccc tcgggctgcc atcgcggcaa cccaagcctt attacgagat ctacctcaag
      541 agtttctgtg ccttgtggaa cgtctcgctg attcgcgacc atgcgatgca tttttcgatg
      601 gcggaccgca cgccgggtta cgagatgcag gaccgcctga ccgcgctcgg ctacaggatc
      661 gtctatatcg cgccgcgcaa gatgttccgt tatctcgatc acgtccagtc aggcacggtg
      721 gcagcagccg gcacttacgg ccaggggcat cgccggacgc gagcctacca ggcgatgctg
      781 aagaagatcg gtcagcgttg agcttcggca tgaaagcgaa gcagttctac ggcagcgaat
      841 ctgcccggca gagcctgcag gagagcggac tcgtcgattt cgaaagcctc tggacgctgg
      901 atgctggctg gttcgaggcg cccaaccagc gtcgcggcgg ctggagcggc gtcagtcgct
      961 atcgcttgaa agacggccac tatgtcttca tcaaacgcca gcagaaccat tccttccgct
     1021 cgtggcgata tctcttccgc gccataccga ccttcacgcg tgagctgagc cgcatccttg
     1081 ggttcgcctc cgcgcatatc cccgcgctcg agctcgtgta tttcggccag cgcgagcatg
     1141 gcaaggatat gcagtcgatc ctggtgacct gggagctggc cgggttcaag tcgctcgacg
     1201 atccggcctt cgttcccttg cgccggctgg atcgcgcctc ccgccatgcg ttgatcgccg
     1261 agaccgcagc cgtcgtgagg cagatgcacg cctatcgcta ccagcataac tgcctgtatc
     1321 caaaacatat cttcgcccgc ctgaatgccg gccccagcgc ggaagtgcgg ctgatcgacc
     1381 tggaaaaagt gaaatggcgg ccagcacggc gttttgccat gctgcgcgat ctcggcactc
     1441 tttatcgtca tgtcgaaggc tggaccgcga ccgatcagtt acggttcttc ctcgcctacc
     1501 ggcaagagtc ggggttgacc gccacatcca agcggatcct gcgcaagatc ctgcgcggaa
     1561 cccgcaaaaa actggcgaga cgcgcctgaa tgccagtaac ggagatgcga tgtcgatagc
     1621 ccgcgatctg cctactcagt tgccggcggt gatcggcgtg gcgggcgcgg cccccttgct
     1681 gccccatgca ttggtgcgct acctgccagg acgccgcgct gtgctgagag gggagtggaa
     1741 tggtcgcgcc gtctacgcca aatggttctt cggcaaacat gcgtcgcgtc acgccctgcg
     1801 tgaccaacgc ggcgtcgagt ggttgaccca ggcacaactg gcgacgccag cgctgctgca
     1861 ctcgggtacg atcgcagaca gcgccgaccg tatcctgatc ttcgccgaaa tgcccggcca
     1921 aagcgtggaa acgctgtggc ccgacctggc gcccgatcag cgtttcggca tcgcccggca
     1981 actggtgacc gaagtcgcgc gccatcaccg tgccgggctg atgcaaaccg atctctattt
     2041 gaagaacttc ctgctgcatg aggatcacct cgtgacgctg gatggtgatg gcgtgcgcag
     2101 gttgcctgtc ttgagggcgg cgcacgccgc ctggcagaac ctcgccttgc tgctgtcaaa
     2161 gttcgaggtg gatgaccagg cgcaatggct tcccccgttg ttggaagcct attgcggagc
     2221 acgggattgc aagccgatag cgctcgacaa gctgaaaagc cgggtcgagc gcgagcggca
     2281 ccgtgtcatt gaccactacg ccgaacacaa ggtcttccgc gcatgttcgg atgtcagcgt
     2341 gagtcgcgaa tggggtagtt tcagtgcgca aatgcgcgac gttttcgccc agccgctgac
     2401 gatcgcgcag atggaggatg cgcttcgaac agggcatccc ctaaagcgcg gcaatacctg
     2461 caccgtcacc cgggcgatga tcggcgatcg aaaagtggtc gttaaaaggt acaatatcaa
     2521 gcactggtta catgggttgg gccgtctttt tcgtcggtcg cgcgcctcgg tctcctgggc
     2581 caacgcgcat cggctgcgta tgcatggcat cgcaaccgcc atgcctttgg ccttggtcga
     2641 gcgccgtttc ggtccctgcc gtctgcaggc atatttcgtt gccgattaca tcgaggcgcc
     2701 ggatatggcg agctggatgg ccgatcgggc aatcgatccg caagacaagg aaacggccgc
     2761 catgggcctt gcgcgactga tgcacaggtt gcgtttgttg cagatcgcgc atggcgacat
     2821 gaaggcgacc aacctccatg tgcatggggt ccggccgatt ttgattgatc tcgacagcat
     2881 gcgccatgtg cgctgccgtc gcagcttcga gcgccggcat
//
LOCUS       DATMTW010000015         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_604868, whole genome shotgun sequence.
ACCESSION   DATMTW010000015 DATMTW010000000
VERSION     DATMTW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604868"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VN084_00290"
     CDS             complement(<1..211)
                     /locus_tag="VN084_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWT27920.1"
                     /translation="MTDHGLPPDAMSMTGMPDAEALLREDAHSREQALAPDSFIVEAP
                     AGAGKTELLTQRYLKLLQQVDDPEEI"
     gene            complement(208..2949)
                     /locus_tag="VN084_00295"
     CDS             complement(208..2949)
                     /locus_tag="VN084_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWT27921.1"
                     /translation="MTAPDLILCSTPRLARSLQVAHGRRQRQAGNRQWMPLPVMTLSA
                     WLDRWLEEALLTGALDAETVPRDVFNPMQERLLWEQAIEASLKHLDSAPLFDKSGLAS
                     AAQEANRLLIEWNLSLPADMQTEETQQFLLWRQRFHSLCKASGWLEPVRYFSWQIEAL
                     ARTSLPLPNRIALAGFDRISPQMQRFIDTLGQRGSAVTPYILGYETSQQARHVTLPDQ
                     DAECRAAVAWAHALLTAHPQARLAIVVPELAALRGKLSAMLDEAFEPTLVMPQLAGQP
                     RLHDFSLGEALTTQAVVADALALLRFGLQRHAVPQAEFSALLHSPYWSASISEADARA
                     RLDTRVREWLPLHVSPQRLLRFTEKHVQGDTALPMPKTLAGLQGLMQASQAQPSRQPP
                     AAWATAFLSLLETAGWPGERSPSSHEYQAMQAFEKVCHALAQLDGVLGMINAGEALRR
                     LTQLCKEQIFQPETEGDPRIQVMGMLEAAAEPLDAVWVMGMNDHVWPPPPRPNPLLPA
                     SLQRAAQTPNADSAAQATFAEAIHARLLHSARELIFSSAEKDGERLLRVSPLMRGIAP
                     AGAEAAYPLQATLAERMAYATEPDSLQLIDDHRAPAVAEGQHLAGGTGLLKAQAICPA
                     WAFYQYRLYARELKLPVTGLDAMERGTLVHAVLECFWQERGSRDVQAMTPEALQAAVA
                     TAAQQALQRFSAERETPFTPAFLALEEARLSKLTLAWLTEIELQRPQPFSVVACEQKQ
                     EIAIEGVAITLVIDRVDALDDGRLIVMDYKTGARNDYRNWAQARITEPQLPVYAAFVL
                     AADDVAAVCFAQIRMDKRGFIGLAAEPELLQGLIALDDKKGREVFAEREFADWPGVLA
                     QWRERIAGIALELKSGEAAVRFTSEKDLVYCEVLPLLRLPERQLQYERLHNDTGEAA"
BASE COUNT          557 a          993 c         1001 g          602 t
ORIGIN      
        1 tgatctcttc cggatcgtcc acctgctgca ataacttgag gtagcgctgg gtcaatagtt
       61 cggtcttacc ggccccggcg ggcgcctcga cgatgaacga atccggcgcc agcgcctgtt
      121 cgcggctgtg cgcgtcttcc ctgagcagcg cttccgcatc aggcatgcca gtcatcgaca
      181 tggcgtcagg cggcaagccg tgatcggtca tgctgcctct cccgtatcgt tgtgcaagcg
      241 ttcatactgc agttgccgct cgggcaggcg cagcaagggc aagacttcgc agtagacgag
      301 atccttctca ctggtgaagc gcactgcggc ctcgcccgac ttcaattcca gcgcgatgcc
      361 ggcgatacgc tcgcgccatt gcgccaatac gccgggccaa tcagcaaatt cacgctcggc
      421 aaagacctcc ctgcccttct tgtcgtcgag cgcgatcaac ccctgcagca actcgggctc
      481 cgccgcgagg ccgatgaacc cgcgcttatc catgcggatc tgggcgaaac agacggcggc
      541 gacatcgtcc gctgccagta cgaacgccgc atagacgggc agttgtggtt ctgtgatgcg
      601 cgcctgcgcc cagttgcgat agtcattgcg ggcaccggtc ttgtagtcca tgacgatcag
      661 ccggccgtca tccagtgcat cgacgcgatc gatcaccagt gtgatggcga cgccttcgat
      721 cgcgatctcc tgcttctgct cgcaggcgac cacgctgaat ggctgtgggc gctgcaattc
      781 gatctcggtg agccaggcca gcgtgagttt actgagacgt gcttcttcca gtgcgaggaa
      841 cgccggcgtg aacggtgtct cacgctcggc gctgaatcgc tgcagggcct gctgggcggc
      901 ggtggcgaca gcggcctgca gcgcctctgg cgtcatggct tgtacatcac gtgaaccgcg
      961 ctcttgccag aaacattcca gcacggcgtg caccagcgtg ccgcgttcca tggcatccag
     1021 tccggtgacc ggcagcttga gttcacgcgc atacaaccgg tattgataga aagcccaggc
     1081 cggacagatg gcctgggcct tgagcagccc ggtaccgccg gcaagatgct gaccctccgc
     1141 gacggcaggc gcccgatggt cgtcgatcag ctgcaggctg tctggctcag tggcataagc
     1201 catacgctcc gccagtgtcg cttgcagcgg ataggcagct tcggcacccg ctggcgcgat
     1261 gccccgcatc aatgggctga cgcgcaacag gcgctcgccg tctttctccg cgctggagaa
     1321 gatgagctcg cgtgcactgt gcaacaagcg tgcgtggatg gcctcggcaa atgtcgcctg
     1381 tgccgcgcta tcggcattgg gcgtctgtgc ggcgcgttgc aggctggccg gcagcagtgg
     1441 attcggccgc ggcggcggcg gccagacatg atcgttcatg cccatgaccc acaccgcatc
     1501 tagcggctct gcagccgctt cgagcatgcc catgacctgg atgcgggggt caccctcggt
     1561 ctcgggctgg aagatctgtt ccttgcatag ttgcgtgagc cggcgcagcg cctcgccggc
     1621 attgatcatg ccgaggacgc catcgagttg cgccagcgcg tggcaaacct tctcgaacgc
     1681 ttgcatcgcc tggtattcat ggctggaagg agaacgttcg cccggccatc cggcggtttc
     1741 cagcaggctg aggaacgccg tcgcccaggc cgctggcggc tgtcttgatg gctgcgcctg
     1801 gctagcctgc atgaggcctt gcagcccggc tagcgtcttt ggcatgggca gggccgtatc
     1861 gccttgcacg tgtttctcgg tgaacctgag caaccgctgc gggctgacat gcaagggcag
     1921 ccattcgcgg acgcgcgtat cgagccgcgc ccgcgcatcc gcctcgctga tgctcgccga
     1981 ccagtaaggc gaatgcagca aggcagaaaa ctcggcttgc ggcacggcgt ggcgttgcaa
     2041 gccaaaacgc agtaaggcca gggcatcggc gaccacagcc tgggtggtga gtgcctcgcc
     2101 cagcgaaaaa tcatgaaggc gcggctgccc cgccagttgc ggcatcacca gcgtcggctc
     2161 gaacgcttca tcgagcatgg cggataactt gccgcgcaag gcggcgagct caggtacgac
     2221 gatggctagc cgggcttgcg gatgtgccgt cagcaaggca tgtgcccagg cgactgccgc
     2281 gcggcattct gcgtcctggt ccggcagcgt gacatgtcgc gcctgttgcg aagtctcgta
     2341 gcccagaatg tatggcgtga cggcgctccc tcgctggccc agggtgtcga tgaagcgttg
     2401 catctggggg ctgatgcggt cgaagcctgc cagcgcgatg cggttcggca gcggcagcga
     2461 ggtccgcgcc agcgcctcga tctgccagct gaagtagcgc accggctcca gccaaccgga
     2521 tgccttgcac aggctatgga agcgttgtcg ccagagcagg aattgctgcg tctcctcggt
     2581 ctgcatatcg gcaggcaagc tgaggttcca ttcgatcagc aggcgattgg cttcctgcgc
     2641 cgctgaagcc agcccgctct tgtcgaacag cggggcacta tcaagatgtt tgagactggc
     2701 ctcgatggcc tgttcccaca gcagcctttc ctgcatgggg ttgaagacgt cgcgcggcac
     2761 cgtttcggca tcgagcgcgc cggtcagcaa ggcttcctcc agccagcgat cgagccatgc
     2821 ggacaaggtc atgactggca aaggcatcca ctgtcgattg cccgcctgcc gttgcctgcg
     2881 accatgcgcc acttgcagac tgcgcgccag gcgcggggtc gagcaaagga taaggtcggg
     2941 ggcagtcatg acgacatttt aactgtgatt cgccggtcgt cactgcggaa atacagcgga
     3001 aatacattgg accgcgaccc atgcctggcg gtcacaaagc ccgcggtctg cgctaacatg
     3061 ctgcatttag catgtcgcgt ttagcctgat gtgttcaggc atgtgcggtt caatcaccct
     3121 ttagatcctt atggcagacc aaaagaacta cat
//
LOCUS       DATMTW010000016         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_235911, whole genome shotgun sequence.
ACCESSION   DATMTW010000016 DATMTW010000000
VERSION     DATMTW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235911"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="ahcY"
                     /locus_tag="VN084_00300"
     CDS             complement(<1..1274)
                     /gene="ahcY"
                     /locus_tag="VN084_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWT27922.1"
                     /translation="MNTVTDIKDYIVADLSLADFGRKEIAIAETEMPGLMAIREEYAL
                     TKPLKGARITGSLHMTIQTAVLIETLKDLGAEVRWASCNIYSTQDHAAAAIAAGGTAV
                     FAIKGETLEQYWDYTHRIFEWPDGGFSNMILDDGGDATLLLHLGTKAETDATVLNHPG
                     SEEEVCLFNAIREKLKVDPKWYSTRLAHIKGVTEETTTGVHRLYQMHKEGRLAFPAIN
                     VNDSVTKSKFDNLYGCRESLVDAIKRATDVMIAGKVAVVAGYGDVGKGSAQALRALSA
                     QVWVTEIDPICALQAAMEGYRVVTMDYAAEHADIFVTATGNYHVITHEHMKKMKDQAI
                     VCNIGHFDNEIDVASLEQYAWDEIKPQVDHVIFPDGKRIILLAKGRLVNLGCGTGHPS
                     YVMSSSFANQTIAQIELFTHSDAYDVGKVYVLP"
     regulatory      complement(1372..1462)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(1505..>2587)
                     /gene="metK"
                     /locus_tag="VN084_00305"
     CDS             complement(1505..>2587)
                     /gene="metK"
                     /locus_tag="VN084_00305"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWT27923.1"
                     /translation="LAQDPYARVAAETLVSTGLVILAGEITTKAQVDYARVARETIRR
                     IGYADPSLRFDADGCSVHVCYGQQSPNIAQGVDRASDDYLNQGAGDQGLMFGYACDET
                     PTLMPLPLYLSHRLVERQSQLRKDGRLPWLRPDAKSQVTVRYVDGKPFAIDTVVLSTQ
                     HSPEYSSDAKQQELREAVIEHIIKPVLPAELIKGEVNYLVNPTGCFEIGGPHGDAGLT
                     GRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLASRCLVQVSY
                     AIGVAKPTSVMVETYGTGKVSDDVLTKLVLEHFDLRPKGIVQMLDLLRPIYTKSAAYG
                     HFGRDEPEFTWEATDKAAALKAAAGI"
BASE COUNT          507 a          763 c          784 g          533 t
ORIGIN      
        1 ggcagcacgt acaccttgcc cacgtcgtag gcgtcgctgt gcgtgaacag ctcgatctgc
       61 gcgatggtct ggttggcaaa ggacgagctc atcacatagc tcgggtggcc ggtgccgcaa
      121 cccaggttga cgagccggcc cttggccagc aggatgatgc gcttgccgtc agggaagatc
      181 acatgatcga cttgcggctt gatctcatcc cacgcgtatt gttcaagcga agcgacatcg
      241 atctcgttat cgaaatgacc gatgttgcag acgatggcct ggtccttcat cttcttcatg
      301 tgttcgtggg tgatgacatg gtagttaccc gtcgcggtga cgaagatatc ggcatgctct
      361 gccgcatagt ccatggtgac gacacgatag ccttccatcg cggcctgcag cgcgcagatc
      421 ggatcgatct cggtcaccca aacctgcgcg gacaatgcgc gcagggcctg tgcagagccc
      481 ttgcccacat cgccatagcc ggccactacg gcgaccttgc cagcgatcat gacgtcggtg
      541 gcgcgcttga tggcgtcgac cagcgattca cggcagccat agagattgtc gaatttagac
      601 ttggtcacgg aatcgttgac gttgatcgcc gggaacgcca ggcgaccttc cttgtgcatc
      661 tgatacaggc gatggacgcc agtggtggtc tcttccgtca cgcccttgat atgggccaga
      721 cgggtggaat accacttggg gtcgaccttg agtttttccc tgatggcgtt gaacaggcag
      781 acctcttctt cgctacccgg atggttgagg acagtggcgt cggtctcggc cttggtgccc
      841 aaatgcaaca acagggtggc gtcgccgcca tcgtccagga tcatgttgga aaaaccacca
      901 tccggccatt caaagatgcg gtgggtgtaa tcccagtatt gctcgagggt ttcgcccttg
      961 atcgcgaaca cggccgtacc gccggcggcg atcgctgcgg ccgcgtgatc ctgcgtggaa
     1021 tagatgttgc aagatgccca acgcacttct gcacccagat ccttgagggt ctcgatcagc
     1081 acggcggtct ggatggtcat gtgcagagaa ccggtgatgc gcgcgccctt caggggcttg
     1141 gtcaatgcat attcttcgcg gatcgccatg agccccggca tttccgtctc ggcgatggcg
     1201 atctctttgc ggccaaaatc agcgaggctg aggtcggcta cgatatagtc tttgatgtca
     1261 gtcacagtat tcatttcgtt taccttgaat gtgtgaccgg agggaaaggc ttgctagaac
     1321 gcgatgtgaa cagcgttttt tcacccatct cccgtgcccg gcacgcgtta atgaaggtga
     1381 acgccgttca caaagcgcat gacgctttga caaccgagcc tggggcggga aagactcccg
     1441 cctcgcagcg ttcctcgatt aatgattgaa ttataaggct aaatatccat ttagccaaac
     1501 cttgctagat accggctgct gccttgagcg ccgcggcctt atccgtggct tcccaggtga
     1561 actcaggctc atcacggccg aaatgaccgt atgcggcgct cttggtgtag atcgggcgca
     1621 acaggtcgag catctgcacg atacccttcg gacgcaagtc aaagtgctcg agcaccaact
     1681 tggtcagcac gtcgtcggac accttgcccg tgccgtaagt ttccaccatc acgctggtcg
     1741 gcttcgccac gccgatggca taggaaacct ggaccaggca gcgcgaagcc agcccagcgg
     1801 cgacgatgtt cttggcgaca tagcgcgcgg cataggcggc cgaacgatcg accttggacg
     1861 gatccttgcc agagaatgct ccgccgccgt gaggcgcggc accgccgtag gtatcgacga
     1921 tgatcttgcg gccggtgagg ccggcgtcac cgtgcggccc gccgatctcg aaacagccag
     1981 tcgggttgac caggtaattc acctcaccct tgatcagctc ggcaggcaac accggcttga
     2041 tgatgtgctc gatgaccgct tcacgcagct cctgctgctt ggcatcagaa ctgtattcag
     2101 gactgtgctg ggtggacagg accaccgtgt cgatggcgaa cggcttgccg tcgacataac
     2161 gcaccgtgac ctggctcttg gcgtccgggc gcagccaggg caagcgtcca tccttacgca
     2221 gttgcgactg gcgctcgacc aggcggtgag aaaggtacag cggcagcggc atcagggtcg
     2281 gtgtctcgtc gcaggcatat ccgaacatca gcccctggtc gcctgcaccc tgattcaggt
     2341 agtcgtcgga agcgcgatcc acgccctgag cgatgttggg gctctgctgg ccatagcaga
     2401 cgtggaccga acagccgtcg gcatcgaacc gcagggaagg atccgcatac ccgatgcgtc
     2461 ggatggtctc gcgcgccacg cgcgcgtaat cgacctgggc cttggtagtg atctcaccgg
     2521 ccagaatcac gaggccagtg gagaccaggg tctcagccgc gacgcgggcg tagggatcct
     2581 gcgccag
//
LOCUS       DATMTW010000017         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_236043, whole genome shotgun sequence.
ACCESSION   DATMTW010000017 DATMTW010000000
VERSION     DATMTW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236043"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..384
                     /locus_tag="VN084_00310"
     CDS             112..384
                     /locus_tag="VN084_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWT27924.1"
                     /translation="MNKQELIDQIAASAELSKADAAKALNAIVDSITHSLKKGEPVSL
                     IGFGTFKVTQRAARTGRNPQTGKEIKIAARKAAGFTAGKALKDAVN"
     gene            404..904
                     /locus_tag="VN084_00315"
     CDS             404..904
                     /locus_tag="VN084_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWT27925.1"
                     /translation="MLNPASITIASLALAIAFSASAAELGGKVVAVKDGDSIVVLDDA
                     FAEHQIRLGGIDAPERSQPHGQAAKRSLSQMVFGQRVLVVWNTTDPYGRTVGKVLLQG
                     EDVNLRQIEAGYAWHYRHYQSEQSPSDRTAYAQAEIDARQAHLGLWQDAAPIPPWRYR
                     RMHRHH"
     gene            complement(907..1917)
                     /locus_tag="VN084_00320"
     CDS             complement(907..1917)
                     /locus_tag="VN084_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWT27926.1"
                     /translation="MSQKLRWGILGAARVNESLLPAIVEAPNAELVAIASRRPGAAAE
                     TLQKYAPKATGVVVHESLEALLADVSVQAVYIPLANEEHGEWTVRAIAAGKHVLVEKP
                     MAITVEDIDAIEAAAARQGVKVMEGFMYRFHPQHQRVREIVSSGLIGEVRSAKASYSF
                     MMRPARLYRLSRDPNHGGGAMWDVGPYAVHSLRHCFDTAPVSVMAMAKYVESGADIAT
                     SGIIDFGHGKYGHFDISFECSRQSEYTIIGTKGSVKCHTVWQLPGTNDVPVISWWSDD
                     GRSAVETYPAANHFNLEIAHFSDCVLNDLPPALSLQDARLNCATIQALLTSAATGKTV
                     KL"
     gene            complement(2079..2155)
                     /locus_tag="VN084_00325"
     tRNA            complement(2079..2155)
                     /locus_tag="VN084_00325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2119..2121),aa:Pro,seq:ggg)
     gene            complement(2239..2640)
                     /locus_tag="VN084_00330"
     CDS             complement(2239..2640)
                     /locus_tag="VN084_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWT27927.1"
                     /translation="MEHAAKVQLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQL
                     KPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARNRLDEHHDAASVDTPAAPSV
                     VSGTSPAAINVDLPDLRQELRQVLALLNPAA"
     gene            complement(2624..2923)
                     /locus_tag="VN084_00335"
     CDS             complement(2624..2923)
                     /locus_tag="VN084_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HWT27928.1"
                     /translation="MTLTKADLADLLFEQVGLNKREAKDMVEAFFEEIRTALEVGDSV
                     KLSGFGNFELRQKSERPGRNPKTGEEIPITARRVVTFHASQKLKSKVEEAYGARG"
     gene            complement(2969..5323)
                     /gene="pheT"
                     /locus_tag="VN084_00340"
     CDS             complement(2969..5323)
                     /gene="pheT"
                     /locus_tag="VN084_00340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWT27929.1"
                     /translation="MQFSEQWLRTFVDPALDSDALSHLLTMAGLEVEEAEPVAAAFSQ
                     VVVAQILSAEKHPDADRLQVCKVDVGAAEPLQIVCGAPNARAGLKAPCALVGAALPGF
                     NIKQAKVRGVDSFGMMCSQKELGLADESDGILELPAEASVGQSIREFLELDDRALTLK
                     LTPNRSDCLSLLGIARDVSALTGAPLSLPEIDAVEVTAGAQRNVTVKESEACPRYAGR
                     MVTGVDAAAATPAWMVRRLERSGLRSISAIVDITNYVLLELGQPMHAFDADRLQGNIE
                     VRYARQGEKLALLNDQAVELDADMLTIADDSGAIALAGIMGGAGTAVSDATRDIFLES
                     AFFAPSVIVGKARRLNFSTDSSYRFERGVDFANTRTALERATALVLEICGGEAGPITE
                     VLNVLPTRPPVTLRMSRLVAALGVTLSAERVAGLFDQLRFDYRQTGDTFQVTPPSYRF
                     DIRIEEDLIEEVARLHGYENIPAIAPHADLHMQPDPESRLNHAVLRDALAAQGYQEIV
                     SYSFVDERWEKELLGNPHPITLKNPIASNMSVMRSGLWGGLIDTLVYNLNRKQERVRL
                     FEIGAAYAASGGAYHETMRISGLAYGDAVPEQWGETARDADFYDIKADVDLLTGHRAQ
                     YAAATHAALHPGQSAQVLLDGKPIGWIGRLHPKWQQHYQLPRGTILFELDVTALLQRE
                     VPRYVEVPKFPPVRRDLAVIVDEDVSVEALMTTMRAHKNTLVSEIGLFDVYRGKGIAE
                     GKKSLAFLVLMQDTQKTLTDAEADEVMAKLLDSIVRQHGAELRK"
     gene            complement(5331..>5712)
                     /locus_tag="VN084_00345"
     CDS             complement(5331..>5712)
                     /locus_tag="VN084_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWT27930.1"
                     /translation="VEGLWVDEHISFANLKGVVQDFLQRFFERDDLQVRFRPSFFPFT
                     EPSAEMDMSWNGGWLEIGGCGMVHPEVFKHVGIDSERYRGFAFGLGVERLTMLRYGVN
                     DLRLFFDNDLRFLKQFNSPAQSNK"
BASE COUNT         1198 a         1707 c         1635 g         1172 t
ORIGIN      
        1 cggacaggcc ttcaaagcaa tattttttca tcctgtcgcc agcatggatg cggctttcgc
       61 taatatctgg gttatgatgg aatctccatc aacctatcag gatagataag aatgaacaag
      121 caagaactca tcgaccagat cgcagctagc gcagaactga gcaaggcaga tgcagccaag
      181 gcgctgaacg ctatcgttga cagcatcacc cactccctga agaaaggtga gccagtcagc
      241 ttgatcggtt tcggtacatt caaggtcacg cagcgcgccg cacgtaccgg ccgcaaccct
      301 caaaccggca aggaaatcaa gatcgctgca cgcaaggcag cgggcttcac cgccggcaag
      361 gcactgaagg acgcagtgaa ctgatcgcgt ctaaattcgg ctagtgttga atcctgctag
      421 catcacgatc gccagcctcg cgctggcgat tgcgttttcg gcgtccgcag cggaactcgg
      481 cggcaaggtg gtcgccgtca aggacggcga cagtatcgtg gtgttggatg atgcgtttgc
      541 cgagcatcag attcgcctgg gcggcatcga tgcgcctgaa cgctcccaac cgcatggcca
      601 agccgccaaa cgatcgttgt cgcagatggt cttcggccag cgcgtactgg tcgtctggaa
      661 cacaacagac ccctacgggc gcacggtggg caaggtgctg ttgcaaggag aggacgtcaa
      721 tctgcggcag atcgaggccg gctacgcctg gcactaccga cattaccaga gcgagcaaag
      781 cccttcagat cgcacggcct atgcgcaggc cgagatcgat gcacgacagg cgcatctcgg
      841 tttgtggcag gatgcagcac cgatcccgcc ctggcgatat cggcgcatgc atcgccatca
      901 ctgactttac agcttgacgg tcttgccagt ggcggcagat gtcagcaagg cctgaatggt
      961 cgcgcaattg agccgagcgt cctgcaggct cagcgctggc ggaaggtcat tgagcacgca
     1021 atcactgaaa tgtgcgatct cgagattgaa gtgattggct gccggatagg tctcgaccgc
     1081 gctacggcca tcgtccgacc accaactgat caccggcacg tcgttggtgc ccggcaattg
     1141 ccagacggtg tggcacttga cgctgccctt ggtcccgatg atggtgtact ccgactggcg
     1201 actgcactca aaactgatat cgaaatggcc atatttgcca tgaccaaagt cgatgatgcc
     1261 gctggtggcg atatccgcac cactctccac atatttggcc atggccatga cgctgactgg
     1321 cgctgtgtcg aagcaatgtc gcagggaatg cacggcatag ggtccgacat cccacatcgc
     1381 gccgccccca tggttagggt cacgtgacaa gcggtagagc cgggctggcc gcatcatgaa
     1441 ggaatagctg gccttggccg atcgcacctc gccgatcagg cccgaactga cgatctcccg
     1501 aacgcgctga tgttgcgggt ggaagcggta catgaatccc tccatgacct tgaccccctg
     1561 cctggcagcg gcggcttcga tcgcgtcgat atcctcgaca gtgatcgcca tcggcttctc
     1621 caccagcaca tgcttgccgg ccgcgatcgc gcgcacggtc cactcaccgt gctcttcgtt
     1681 ggccagcggg atatacacgg cctgcacgct gacatcggca agtagcgcct ctagcgattc
     1741 gtgcaccacg acaccggtag ccttgggcgc atatttctgt aaagtctcgg ccgcagcgcc
     1801 tggacggcga ctggcaatgg cgaccagttc ggcattcggg gcttcgacga tggctggcaa
     1861 caagctctca ttgacgcgtg ccgcgcccag gataccccag cgtaatttct gactcatggc
     1921 ggaacctctt ggatgacggt gtgattgaat gacgttagct aaacccagag tttaacgcat
     1981 cccttccacg ttatcgccgg gcttgtcacc gagcaggacc cgaaggccct tgaaaacaaa
     2041 aaagctgcca agggcagctt ttttgtttat gtgttctttg gtcggggtga gaggatttga
     2101 acctccgacc acttgcaccc catgcaagta cgctaccagg ctgcgctaca ccccgaaccg
     2161 ttgccgcata ccattcgcag caagccgacc attatagcgt aaagccccga ccgggaaaac
     2221 cagagaaccg cttagtgctc aagcagcggg gttaagcaag gccagtactt ggcgcagttc
     2281 ctggcgcaag tctgggaggt cgacgttgat ggcggcaggt gatgtgccgg agacgaccga
     2341 cggcgcagcg ggcgtatcca ccgaggccgc gtcatggtgc tcgtcgaggc ggttgcgcgc
     2401 accgctgatg gtgaaccctt gttcatagag caactcgcgg atgcgacgga tcagcaggac
     2461 ttcgtgatgc tggtaatagc ggcgattacc gcgacgcttg accggcttga gctgggtgaa
     2521 ctcctgctcc cagtaacgca gcacatgtgg cttgacacca cacagctcac tgacctcacc
     2581 gatggtgaag tagcgtttgg ccgggatcgg cggtaactgg actttagccg cgtgctccat
     2641 acgcctcttc taccttgctt ttgagcttct ggctggcatg gaaagtaacg acgcgacgtg
     2701 cggtgatcgg gatctcttcc ccggttttag gattgcggcc tggacgttcg gatttttggc
     2761 gcaattcaaa attgccaaaa cccgagagct tgacgctatc gccaacttcg agcgcagtgc
     2821 ggatctcttc gaaaaaagct tcaaccatgt ctttggcttc acgcttgttc agaccgacct
     2881 gctcaaacag caaatcggcc aaatcagctt tagtcagtgt cattcaaacc tctcaaacta
     2941 cttatttgat ttttgcttgt gttgttatct attttcgtag ttctgcgccg tgctggcgaa
     3001 ctatggaatc gagcagtttt gccatcactt catcggcttc tgcatccgtc agggtttttt
     3061 gagtatcttg cataagcacc agaaaagcaa ggcttttttt gccttcggcg atgccttttc
     3121 cgcggtagac atcgaacaat ccgatctcgc tgaccaaggt gtttttgtgg gcgcgcatcg
     3181 tcgtcatgag cgcctcgacc gagacatctt catcgacgat gaccgcgagg tcacgacgca
     3241 ccggcgggaa cttcggcacc tctacatagc gcggcacttc gcgttgcagc aatgccgtca
     3301 catcaagttc gaacaatatc gtgccgcgcg gcagctggta atgctgttgc cacttggggt
     3361 gcagtctgcc gatccagccg ataggcttgc cgtcgagcag cacttgcgcc gactggcctg
     3421 gatgcaaggc cgcatgggtc gctgccgcat actgcgcacg atgacccgtc agcagatcga
     3481 catcggcctt gatgtcatag aaatcagcgt cacgcgcggt ctccccccat tgttccggta
     3541 cggcatcgcc ataggcgagg cccgagatac gcatggtctc gtgataagcg ccaccagacg
     3601 ccgcgtaggc tgcgccgatc tcgaacaggc gcacacgctc ctgcttgcgg ttgaggttat
     3661 agacaagagt atcgatcaac ccaccccaca gaccagaacg catgacgctc atattgctgg
     3721 caatagggtt cttgagagtg atcggatgcg gattgccgag caattccttt tcccagcgct
     3781 cgtccacaaa gctatagctc acgatctcct ggtagccttg cgccgccaat gcatcgcgca
     3841 acacggcatg gttgagccgg ctttcgggat caggctgcat atgcaggtca gcatgcggcg
     3901 cgatggccgg gatattctcg tagccatgca aacgggcgac ctcttcgatg agatcttcct
     3961 cgatgcggat atcgaaacga tagctgggcg gcgtcacctg gaaagtatcg cccgtctgtc
     4021 gatagtcgaa tcgcagctga tcgaacaggc cagcgacccg ctcggcgctg agcgtgaccc
     4081 ccaatgccgc gaccagtcgc gacatacgca acgtaacagg cgggcgtgtc ggcaacacgt
     4141 tcaagacttc ggtgatcggt ccggcttcgc caccgcatat ctcgaggacc aaggccgtgg
     4201 cgcgctctag cgccgtcctg gtattggcga aatcgacccc gcgttcgaat cggtaagaag
     4261 aatccgtaga gaagttcaaa cgccttgcct tgccgacgat cacgctcggc gcgaagaagg
     4321 cactctccag aaagatgtcg cgggttgcat ccgaaacggc ggtgccagca ccgcccatga
     4381 tacccgccag cgcgatggcg ccgctgtcgt ctgcgatggt cagcatgtcg gcatcgagtt
     4441 cgaccgcctg gtcattcagt agcgccagct tttcgccctg gcgcgcatag cgcacttcga
     4501 tattgccttg cagcctgtcc gcatcgaagg cgtgcatcgg ctgcccgagt tccagcagca
     4561 cataattggt gatatcgacg atcgcactga tactgcgcaa gccgctgcgc tcaaggcgcc
     4621 tgaccatcca cgccggcgtc gcggcggcgg catcgacgcc agtcaccatg cgtccggcat
     4681 agcgtgggca ggcttccgat tccttgacgg tgacgttgcg ttgcgcgcct gccgtcactt
     4741 cgacggcgtc gatctcgggc agggacaatg gcgcgccggt cagcgccgac acatcccggg
     4801 caatgcccag gaggctcagg cagtcactgc ggttcggcgt gagcttgagt gtcagggcac
     4861 ggtcgtccag ctccaggaac tcacggatac tttgtcccac cgaggcttcg gccggcaatt
     4921 ccaggatgcc gtcgctctcg tccgccagcc ccagctcctt ctgtgaacac atcatgccaa
     4981 aggagtcgac gccgcgcacc ttggcctgct tgatgttgaa tcccggcaat gccgcgccga
     5041 ccagtgcaca aggcgccttg agtccggcac gcgcgttcgg cgcaccgcag acgatctgca
     5101 acggttcggc agcgccaacg tcaaccttgc agacctgcag gcggtcggca tccggatgtt
     5161 tttcggcact gagtatctgg gcaacgacca cttggctgaa tgctgcggcc acaggttcag
     5221 cttcttcgac ctcgaggccg gccatggtca agaggtgact caaggcatcg ctgtcgagtg
     5281 ccggatcgac gaaggtacgt agccactgtt cggagaattg catgtcgttc ctacttgttc
     5341 gattgtgctg gcgaattgaa ttgcttcagg aatctgagat cgttgtcgaa gaaaaggcgc
     5401 aaatcgttga cgccatagcg cagcatggtc aggcgctcga cgccgagccc aaaggcaaaa
     5461 ccacggtatc gctcgctatc gatgccgacg tgcttgaaca cttcaggatg caccatgccg
     5521 caaccgccga tctcaagcca gccgccgttc cagctcatat ccatctcggc cgacggttcg
     5581 gtgaacggga aaaaggatgg acggaaacgc acttgcaggt cgtcacgttc gaagaaacgc
     5641 tgcaggaaat cctgcaccac acctttgagg ttagcaaaac tgatgtgctc gtcgacccat
     5701 aatccctcta cc
//
LOCUS       DATMTW010000018         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_537956, whole genome shotgun sequence.
ACCESSION   DATMTW010000018 DATMTW010000000
VERSION     DATMTW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537956"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VN084_00350"
     CDS             complement(<1..945)
                     /locus_tag="VN084_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWT27931.1"
                     /translation="MDNIPISLQLGVLAILLMISAFFSIAETSLMSVNRYRVRHLAKD
                     GHRGARLTVSLLNRTDKLLGMILLGNNLANAASATLVALITVELFGEGQWIVLMGTLA
                     VTFAILVFSEISPKVIAAAKPEKFAFACSYVLYPLLWLTRPVVWFVNLFVSALLRLLG
                     IKVNFAESTQAITMEELRSIVIDAGSYIPKKHRAILLNLFELEKTTVDDVMTAHTQIE
                     SIDFDTPLEDILQHIASSHHTRLPVREGPTEEIIGILHIRKVMNQLRNGELDLEALRE
                     VISEPYFIPCGTPLYTQLQQFQEHRQRIALIVDEYGELK"
     gene            complement(1018..>1822)
                     /gene="secF"
                     /locus_tag="VN084_00355"
     CDS             complement(1018..>1822)
                     /gene="secF"
                     /locus_tag="VN084_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWT27932.1"
                     /translation="FTGGTVMEVSYPQAANLEQVRSAVDSVGLKEATVQNFGTSKDVL
                     IRLPVKEGLTSAQLSEKVLNALRNVDGSVEMRRVEFVGPQVGQELYDNGALAMLLVCI
                     GIMGYLAMRFEWRFAVAAVIANMHDVVIILGFFAFFQWEFNLTVLAAVLAVLGYSVNE
                     SVVVFDRVRENFRKMRKAAVPEIINNAITRTMSRTIITHAMTQTMVCSMLFFGGEVLH
                     NFSLALTIGILFGIYSSVLVASPIAMYLGVSRENLITGAEKKPQAEAIP"
BASE COUNT          372 a          494 c          596 g          360 t
ORIGIN      
        1 cttgagttca ccgtattcgt cgacgatcag cgcgatgcgc tggcgatgct cctggaactg
       61 ctgtagctgc gtgtagagtg gcgtcccgca gggaatgaaa tagggttccg agatgacttc
      121 gcgcaaggct tccagatcga gttcgccgtt gcgcagctgg ttcatgacct tgcggatatg
      181 caggatgccg atgatctctt ccgtcggtcc ctcgcggaca ggcaggcgcg tatggtggct
      241 gctggcgatg tgttgcagga tatcttccag tggcgtatcg aaatcgatgg attcgatctg
      301 ggtatgggcg gtcatgacgt cgtccaccgt ggtcttttcc agctcgaaca agttcagcag
      361 gatggcgcgg tgcttcttcg ggatgtaact gccggcgtcg atgacgatgc tgcgcagctc
      421 ttccatggtg atggcctgcg tcgattcggc gaaattgacc ttgatgccga gcagacgcaa
      481 cagggcgctg acgaacagat tgacgaacca gaccacaggc cgggtcagcc agagtagcgg
      541 gtagagcaca tagctgcagg caaaggcgaa tttctcgggc ttggcggcgg cgatgacttt
      601 gggcgagatt tcgctgaaga caaggatggc gaaggtgacg gcgagggtgc ccatgagcac
      661 gatccattgg ccctcgccga acagctcgac cgtgatcagc gcgaccagcg ttgcggaggc
      721 tgcattggcc aggttgttac cgagcaggat catgcccagt aacttgtccg tgcgattgag
      781 cagtgacacc gtaagccgtg cgccgcgatg gccatccttg gccaggtggc gcacgcggta
      841 acggttgacc gacatcaggc tggtttcggc gatggaaaag aatgccgaga tcatgagcag
      901 aatggccagt acgccaagct gcagggagat gggaatgtta tccaaggagg tcaggccaag
      961 taggcgatgc cctagtttcc gcgggaagcg cagcccttgt caaggctgcg caggcgatca
     1021 cgggatggct tcggcttgtg gcttcttttc ggcgccggtg atgaggttct cgcgcgagac
     1081 gccaagatac atggcgatcg ggcttgcgac cagcaccgag gaatagatac cgaacaggat
     1141 gccgatggtc agcgccagcg agaagttgtg caggacctca ccgccgaaga acagcatcga
     1201 gcagaccatg gtctgtgtca tggcgtgcgt gatgatggta cgcgacatgg tgcgcgtgat
     1261 ggcgttgttg atgatctccg gtacggcggc cttgcgcatc ttgcggaagt tttcgcgcac
     1321 ccggtcgaac accaccacgg attcgttcac cgaatagccg agtacggcca gcaccgcggc
     1381 cagcacggtg agattgaatt cccactggaa gaaggcgaag aagcccagga tgatgaccac
     1441 gtcatgcatg ttggcgatga cggcagcgac agcgaaacgc cattcgaaac gcatggcgag
     1501 gtagcccatg atgccgatac agaccagcag catggccagg gcgccgttgt cgtagagttc
     1561 ttgcccgacc tgcgggccga cgaactcgac gcggcgcatc tccaccgaac catcgacatt
     1621 gcgtagcgca ttgagcacct tttccgagag ctgggcactg gtcaggcctt ccttcaccgg
     1681 caggcggatc aggacgtcct tgctggtgcc gaagttctgt accgtcgctt ccttgaggcc
     1741 gacgctatcc accgcggagc gcacctgctc caggttcgca gcctgcggat agctgacttc
     1801 catgacggtg ccgccggtga ag
//
LOCUS       DATMTW010000019         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_169157, whole genome shotgun sequence.
ACCESSION   DATMTW010000019 DATMTW010000000
VERSION     DATMTW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169157"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN084_00360"
     CDS             <1..241
                     /locus_tag="VN084_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWT27933.1"
                     /translation="LLVLDANTGQNAVMQVKAFDDALKVSGLVLSKLDGTAKGGVIAA
                     IAQTRPIPIRYIGVGESIGDLRSFKATEFVDALFD"
     gene            413..1063
                     /gene="ftsE"
                     /locus_tag="VN084_00365"
     CDS             413..1063
                     /gene="ftsE"
                     /locus_tag="VN084_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWT27934.1"
                     /translation="MIKFDQVGKRYPGSNLALNGISFEIELGDLVFVTGHSGAGKSTL
                     LKLIAGIERPTSGTVLIGNQNISQLRPAALPYLRRKFGLVFQDHKLLYDRNCFENVAL
                     PLYINGVTGSEAAKRIRAALDKVGLLNKERAMPITLSGGEQQRLAIARAVVSRPSILI
                     ADEPTGNLDETYAGEILSLFHAFHQVGVTVIVATHDAQGISSEHKILRLNHGALAA"
     gene            1060..1962
                     /gene="ftsX"
                     /locus_tag="VN084_00370"
     CDS             1060..1962
                     /gene="ftsX"
                     /locus_tag="VN084_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWT27935.1"
                     /translation="MKQWLNQHKQALRAVVVRMRHNLLATLMMFGVMGVALSLPGVLY
                     VVADNLSRLAGSVQNEPQISLFLKLDIDAETVAAIEQKLKTHPDVAHYQFVSRDAAWE
                     QLQQSAQAVATSIDKNPLPDAYFIEPKELKPETVERLLNEMQQWPGVEHAQADAAWIK
                     RLHAVLGLGRNAAAVLGGLLGLALIAIIGNTIRLQILTQREEIEVSQLIGATDRFIRR
                     PFLYAGTLYGLGGGLAAWLLLAGIISLFNYSVTDIAELYAANFTLELPSLAVNLAMIA
                     SAVILGWIASYIAVSRALASLQGR"
     gene            2121..>2612
                     /locus_tag="VN084_00375"
     CDS             2121..>2612
                     /locus_tag="VN084_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27936.1"
                     /translation="MSVPVWELPRRADYTLQINISRNTLIAVCFSILLHLLLLLWLKP
                     TILLPDGAQGKPQPITVTLNAPIKPAAPQAEAVVEPPPPVVQPHPAKPKPVRSRPQKT
                     QPDVMAVEKVTPDAIAKVPAPASTPEPEAAPTDMMSYVKAAQAKRQAAENYAAQINAD
                     AAAR"
BASE COUNT          545 a          827 c          764 g          476 t
ORIGIN      
        1 gctgctggtg ctcgatgcca acacggggca gaatgcggtg atgcaggtga aggccttcga
       61 tgacgccctc aaggtcagcg gcctggtcct gagcaagctc gacggcacgg ccaagggcgg
      121 cgtgatcgct gctatcgcgc agacgcggcc gatcccgatc cgctacatcg gcgtcggcga
      181 atcgatcggc gacttgcgtt cgttcaaggc cactgaattc gtggatgcac tgtttgattg
      241 agccgagcaa tgccgaacgc atgccggcat cgatggcgcg gtttggcgat acgccaagac
      301 ccggtgccag cgcagacgcg gcacacctca ttttgacagg cgcggagttg gccggcgctt
      361 cgttggccgg tgaagagaca ccagacaaaa caaagggatt tcagggacgt ccatgatcaa
      421 gttcgaccaa gtcggcaaac gctaccccgg tagcaacctg gcattgaacg gcatcagttt
      481 cgagatcgaa ctgggcgatc tcgtgttcgt cactggtcat tcaggtgcgg gcaagagcac
      541 cttactcaaa ctgatcgcgg gcatcgagcg gccgaccagc ggcacggtgc tgatcggcaa
      601 ccagaacatc agccagctgc gcccggctgc attaccttat ttgcgtcgca agttcggttt
      661 ggtgttccag gaccacaagc tgctctatga ccgaaactgc ttcgagaacg tcgccctgcc
      721 gttgtatatc aacggtgtca ccggcagcga agccgccaag cgcatccgcg ccgcactgga
      781 caaagtaggc ctgctgaaca aggaaagagc catgccgatc accttgtctg gtggcgagca
      841 gcaacgcctc gcgatcgcgc gcgccgtggt gagccggcct tcgatcctga tcgctgacga
      901 gcccacgggc aatctggacg aaacctatgc cggcgagatc ctgtcgctgt tccatgcctt
      961 ccatcaagtc ggcgtgacgg tgatcgtggc cacgcacgac gcgcaaggca tctcgtcgga
     1021 acacaagatc ctcagactca atcatggagc gctggccgca tgaaacaatg gctcaaccag
     1081 cataagcagg cgctgcgcgc cgtcgtcgtc cgcatgcggc acaacctgct ggcgacgctg
     1141 atgatgttcg gcgtcatggg cgtcgccctg tcgctgcccg gggtgctgta tgtcgttgcc
     1201 gacaatctca gtcgactggc tggcagcgta cagaacgagc cgcagatcag cctgttcctc
     1261 aaactggata tcgatgccga gaccgtggct gcgatcgagc agaagctgaa gacgcatccg
     1321 gatgtggcgc attaccaatt cgtttcacgc gacgccgcct gggagcagct acagcaaagc
     1381 gcacaggcgg tcgcaaccag tatcgacaag aatccgttgc ccgacgctta cttcatcgag
     1441 cccaaggaac tgaaacctga gaccgtggag cgactgctga acgagatgca gcaatggcca
     1501 ggggtggagc acgcacaggc cgatgccgcc tggatcaaac gcctgcatgc ggtcctgggc
     1561 cttggccgca acgcggctgc agtgctaggt ggactgcttg gattggcgct gatcgccatc
     1621 atcggcaaca ccatccgctt gcagatcctg acgcagcgcg aagagatcga ggtcagccag
     1681 ctcatcggcg ccaccgatcg cttcatacgc cgccccttcc tatatgcggg caccctgtat
     1741 ggcttgggcg ggggcctggc ggcatggttg ctgctggcag ggatcattag cttattcaat
     1801 tattcggtga ccgatatcgc cgagctctac gcggccaatt tcacactgga actgccgagc
     1861 ctcgccgtca acctggccat gatcgcgagt gccgtcatcc tgggctggat cgcctcctac
     1921 atcgccgtca gccgggccct ggccagtcta cagggccgct gaaccacacc tgatcggacg
     1981 cgcaagacaa gcctggttgt gcaaggattt tttctgctgt ttgacggtct ctcctcattt
     2041 aaacgcctat aggatcgcgc ttgtcccaga aaccggcttc cgctgcagtg caggcaaccc
     2101 agtcgtcctc agcgtccggg atgtctgtgc ctgtctggga gctgcctcgt cgcgccgact
     2161 acaccctgca gatcaacatc agccgcaaca cattgatcgc ggtctgcttc tcgatcctgc
     2221 tgcatctgct gctcctgtta tggctcaagc ctactatcct cttgccggat ggcgcgcaag
     2281 gcaaaccgca acccatcact gtcacgctca acgcgccgat aaagccagcg gcaccgcaag
     2341 ccgaagcggt tgtcgaaccg ccgccccccg tagtacaacc acacccggcg aagccgaaac
     2401 ctgtgcgctc cagaccgcag aagacacaac ccgatgtcat ggcggtagaa aaagtcacgc
     2461 cggacgcgat cgctaaagtg ccagcgccgg cctcgacgcc cgagcccgaa gccgcaccga
     2521 ccgacatgat gtcttacgtc aaagccgcgc aagccaagcg tcaagctgcc gagaactacg
     2581 cggcccagat caacgccgat gcggcggcac gt
//
LOCUS       DATMTW010000020         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_68769, whole genome shotgun sequence.
ACCESSION   DATMTW010000020 DATMTW010000000
VERSION     DATMTW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68769"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..377)
                     /locus_tag="VN084_00380"
     tRNA            complement(301..377)
                     /locus_tag="VN084_00380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Arg,seq:ccg)
     gene            492..1004
                     /locus_tag="VN084_00385"
     CDS             492..1004
                     /locus_tag="VN084_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWT27937.1"
                     /translation="MGEQHHDFPKTTVSQVIIATLGGLFAPALVIFLVVKLLLGIQAN
                     HLEDTDPAPQAAAVEARIAPVGDVEVVAAEAGPHVDKSGEEVVGAVCSACHASGALGA
                     PKIGDKAAWGPRIAQGYETLIKHAIAGIRSMPPRGGNASLTDGEIANAIAHMANQSGA
                     HFTPPAPKSE"
     gene            1056..1895
                     /locus_tag="VN084_00390"
     CDS             1056..1895
                     /locus_tag="VN084_00390"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWT27938.1"
                     /translation="MAIYAVGDIQGCYFSFQRLLEHLDFNPDHDRLWLVGDLINRGAG
                     SLATLRWLHAHRDSVTCVLGNHDLHTLAVAEGFVKAHRSDTLDALLTAPDSETLLTWL
                     RHQPMIHRGEGYVMVHAGLLPQWDIEQALTLGREVEAVLQGAGYRDFLACMYGNQPDL
                     WQDDLAGYDRLRLITNAMTRLRVCSAEGRIDFRFKGELDQIPAGCLPWFDVPGRLSTD
                     SVIVCGHWSALGLRQRQNLYTLDTGCLWGGQLTAMRLEDRAIFQAPCDLRDSPRKIEP
                     AED"
     gene            complement(1863..2624)
                     /locus_tag="VN084_00395"
     CDS             complement(1863..2624)
                     /locus_tag="VN084_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWT27939.1"
                     /translation="MQNRPTSRFIRLFRIARMMAHTLYGALAAVTILPWIGPRGRDLL
                     ISHWCRGLLSCLNIQVRVTGTPPTIDCQRTMFVANHISWVDIHALNSIRAVRFIAKAE
                     IRDWPVFGWFAYKVNTLFIERAKRHDTGRIVELASESLRAGDNLCFFPEGTTTDGTHV
                     KPFKSSLLQAAVNAEARIWPFSIHYPGPDGTPNIQMAYHDDVTLMQSIQQVLAQPTPV
                     VHLHFSSPLEAGGQDRRTLLTQARASILSGLDLPG"
     gene            complement(2634..3383)
                     /locus_tag="VN084_00400"
     CDS             complement(2634..3383)
                     /locus_tag="VN084_00400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HWT27940.1"
                     /translation="MLERHSSTGLHQESGHLYFSFARSPDEIAEAQRLRYKVFAEEMG
                     ANIPGKDGHDRDGFDVFCDHLLVRDSLTSEVVGTYRILNPAMANEAGGYYSSGEFDIS
                     RLSHLFDRTVEVGRACVHQNYRHGGTITMLWAGLAKYMQINRYEYMIGCGSVGMADGG
                     HMAASLYNSLRNDYLSPPEYRVFPRCPLPLEALQSDMEVTLPPLIKGYLRLGAYICGE
                     PAWDPDFNTADMLVMLPLSRLNRRYAAHFFK"
     gene            complement(3491..>3712)
                     /locus_tag="VN084_00405"
     CDS             complement(3491..>3712)
                     /locus_tag="VN084_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27941.1"
                     /translation="GAGQAIATVVGAIVGGIAGAAAEEGITRKDGIEITVKLDSGPLV
                     AIVQEADEVFQPGEKVRLIESGGTTRVSH"
BASE COUNT          745 a         1054 c         1150 g          763 t
ORIGIN      
        1 cgtgctctgc ttctgcataa gcggcttgtg gcataccagc accgccttgc taaccatcgc
       61 aaaactggtt gcaaacctac cactggcgcc atggatggtc gcaacatcaa acagggagtg
      121 gaattgccgg ataagcgaat cttggtcggg taagcactgg atatcccaaa ttcgtcatgc
      181 atcagatttg cgagcaaatg cggcatagag tgagtggcac gaacttgctg atgacggctc
      241 tatttaacct ggggatgtaa cgtggggctg ccgcagaaac cctggggtca tgacgatgta
      301 tggcgcgccc ggcgggagtc gaacccacga cctttggctt cggaaaccaa cactctatcc
      361 agctgagcta cgggcgcctg tgagagaagc aggcggatta tagcaaatag ggtatgcctt
      421 gtcccttttg cgtcgctggc attcccagta taatcagcgg ctttcgcact tgctattagg
      481 aatacattgt catgggtgag cagcatcacg attttccgaa aacgacggta tctcaagtca
      541 tcatcgcaac cctgggcgga ttgtttgccc ctgcgttggt catcttcctc gtggtgaagc
      601 tgctgctggg catccaggcc aaccatctcg aagacactga tcctgcacca caggccgcag
      661 ccgtcgaagc gcggatcgcg cctgttggcg acgtggaagt cgtggctgcc gaggcgggcc
      721 cgcacgttga caagagcggg gaagaagtgg tcggcgcggt ctgttccgcc tgccatgctt
      781 ccggagcgct cggtgccccc aagatcggtg acaaggctgc atggggcccc cgtatcgctc
      841 aaggttacga gacgctgatc aaacatgcca tcgcaggtat tcgttcgatg ccacctcgtg
      901 gtggcaacgc gagcctgacc gacggtgaga tcgctaacgc catcgcgcac atggcaaacc
      961 aatccggcgc gcatttcacg ccgccagcac ccaagtcaga ataaggcttc aagcccaggc
     1021 cgccgccaag gcggcctgcc agcaccggga taccgatggc aatttatgca gtaggggaca
     1081 ttcagggctg ctacttctca ttccagcgat tgctggaaca tctcgatttc aatcctgatc
     1141 acgaccgcct gtggctggtc ggcgatctca tcaaccgcgg cgccggttcg ctggcgactt
     1201 tacgctggtt gcacgcacat cgtgacagcg tcacctgcgt gcttggcaat catgacctgc
     1261 acacacttgc agtcgccgaa ggtttcgtga aagcccatag aagcgacacc ctggacgcat
     1321 tgctgacagc gccggatagc gaaaccctgc tgacctggtt gcgtcaccag cccatgatcc
     1381 accgcggcga gggttacgtc atggtacatg ccggcctgtt accccagtgg gatatcgaac
     1441 aggcgctaac cttggggcgt gaggtcgagg ccgtgctgca gggggctggc tatcgggatt
     1501 ttcttgcctg catgtacggt aatcagcctg atctctggca ggatgatctg gcaggctacg
     1561 atcggcttcg cctcatcacc aacgcgatga cccgcttgcg cgtatgcagc gccgaggggc
     1621 gcatcgattt cagattcaag ggcgagctcg accagatccc ggcaggttgc ctgccctggt
     1681 tcgatgtgcc gggccgcctg tctacggata gcgtgatcgt ttgcgggcac tggtcggcgc
     1741 tgggcttgcg tcagcgccag aatctttaca ctctggatac cggttgtctg tggggcggac
     1801 agctgacggc gatgcggctg gaagatcgcg cgatcttcca ggcgccgtgc gacttgcgcg
     1861 attcaccccg gaagatcgag cccgctgagg attgacgccc gcgcctgcgt tagcagcgtc
     1921 cggcggtcct ggccgccagc ttcaagcggc gatgagaaat gcagatggac gacgggtgtc
     1981 ggctgggcga gcacttgctg gatggattgc atcagcgtga cgtcgtcgtg gtaagccatc
     2041 tggatgttcg gtgtgccgtc ggggcccgga taatggatgc tgaacggcca gatacgtgct
     2101 tcggcattga cagccgcctg tagcaggctg cttttgaatg gtttgacatg ggtgccgtct
     2161 gtcgtggtgc cttccggaaa gaaacacaga ttatccccag cccgcaggct ctcgctcgcc
     2221 agctcgacga tgcgtccggt gtcgtgtcgc ttggcgcgtt cgatgaacag ggtgttgacc
     2281 ttgtaggcga accagccgaa caccggccag tcacggatct ccgctttggc gatgaaacgc
     2341 acggcacgaa tgctgttgag ggcatggata tcgacccacg agatgtggtt ggcgacgaac
     2401 atggtgcgct ggcaatcaat ggtcggcggc gtgccagtca cccggacctg gatattgagg
     2461 catgacaaga gcccgcgaca ccagtggctg atcagtagat cgcggcccct gggaccgatc
     2521 cagggcagga tcgtcactgc ggccagcgcg ccgtaaagcg tgtgggccat catgcgggcg
     2581 atacggaaga ggcggatgaa gcggctggtc ggtctgttct gcaagcgaac cctttatttg
     2641 aagaagtgcg ccgcgtatct cctgtttaac cgcgacaatg gcagcatgac cagcatgtcg
     2701 gcggtattga aatccggatc ccaggccggc tctccgcaga tgtaggcacc gaggcgcaga
     2761 tagcccttga tcaacggcgg caaggtgact tccatgtctg actggagcgc ttccagcggc
     2821 aaagggcaac gcgggaacac acgatactct ggcggcgata agtaatcgtt gcgcaaacta
     2881 ttatagaggc tggcggccat gtgtccacca tcggccatgc cgacgctacc gcagccgatc
     2941 atgtattcat agcgattgat ctgcatgtat ttggcgaggc cagcccagag catggtgatg
     3001 gtaccgccgt ggcggtaatt ctgatggacg caggcacgcc cgacttccac ggtccggtcg
     3061 aaaagatgcg acaggcggct gatatcgaat tcgccggatg agtaatagcc accggcctcg
     3121 ttggccatgg ctggattgag gatgcggtag gtgccgacga cttcgctggt caggctgtca
     3181 cgtaccagca agtgatcaca aaagacatcg aagccatcgc ggtcatggcc atccttgccc
     3241 gggatgttcg cgcccatctc ttccgcgaag accttgtaac gcaagcgctg ggcctcggcg
     3301 atctcgtccg ggctacgcgc aaagctgaaa tagagatggc ctgattcttg gtgcaggccg
     3361 gtggaggaat gccgttcaag catcgtgttc ccctgactgg ttgatgagac aggggaactt
     3421 tacgaagctt gcgtgacgga ttagtgacta atcgttgaag gttcgatgac ggaaagtccg
     3481 ggcaactaga ctagtggctg acgcgagtcg tgccgccgct ttcgatcagg cgtacctttt
     3541 cgccgggctg gaagacttca tccgcttcct ggacgatagc gaccagtggc ccgctgtcga
     3601 gtttgacggt gatctcgatg ccatccttgc gggtgatacc ttcttccgcc gcggccccgg
     3661 cgatgccgcc gacaatggcg ccaacgacag tcgcgatcgc ctggcccgca cc
//
LOCUS       DATMTW010000021         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_68883, whole genome shotgun sequence.
ACCESSION   DATMTW010000021 DATMTW010000000
VERSION     DATMTW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68883"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VN084_00410"
     CDS             <1..351
                     /locus_tag="VN084_00410"
                     /inference="COORDINATES: protein motif:HMM:NF040395.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HWT27942.1"
                     /translation="DHSHADEIDNEAMAKSFQAAGVPVGTLPGGTESILQEAQRIIHG
                     PRRDGYGHPRKNFADTAAVWSVLLGTTVTSRQVGQCMIALKLCRDMKKPGRDNLTDIA
                     GYAGCMELIDEAQS"
     gene            348..1481
                     /locus_tag="VN084_00415"
     CDS             348..1481
                     /locus_tag="VN084_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27943.1"
                     /translation="MSNARMTAEQAREYLATWAKHGFSCAATARATGLSRTTWSDRIS
                     TAKIRCPDSGIVPTDVPDGMKFTKTTVQYDGAGNVIQEWRRLHKDAESMEALVEALAA
                     GLKGKCPALPRPAKPVNNKRTLVFPIYDPHFGKHAWEAETGENYDIKIAERVLVNTAR
                     IAMERSGPLDEVVFILGGDWFHSDTRTPQTERSGHVLDVDTRSERVRDVTIKAIVAAI
                     AIFSAVANKVHVVCIPGNHDHESMHWTARILEAVYAGQKHITVDRSPRSRRYLKRGKM
                     LLGIDHGMMKINEYLTLMPCEAADLWATTTERLWLLGHIHQQKVIQKHGVTVEHLESI
                     SATDAWHAESGYVGNPRRTVTFLIDPSYGLVSRSYITVQESMQ"
     gene            1478..>2009
                     /locus_tag="VN084_00420"
     CDS             1478..>2009
                     /locus_tag="VN084_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27944.1"
                     /translation="MTPVELTHHLQRLRRSDHWYKHRAAAAVDLNWYEVIPSRSENSV
                     YLVRFWLNKPAIDGSGKLDSSNATLLHWFVSHDDDGALHDHPWDFSTTVLAGGYIETT
                     PARKPGRLGPGTFKATTVTAGATLQHEAADLHAIAGLLGETWTLVTTGPRIREWGFHP
                     PGQAWADWRTFLAPKRG"
BASE COUNT          412 a          664 c          606 g          327 t
ORIGIN      
        1 gaccacagcc acgctgatga gatagacaac gaggcgatgg cgaaaagctt tcaggctgct
       61 ggtgtgccgg tcggcacgct gcctggcggg actgagtcga tccttcagga ggcgcagcgg
      121 atcatccacg ggccgcgacg cgatggctat ggtcaccccc gaaagaactt cgccgacacc
      181 gccgccgtct ggtccgtgct gcttgggacc acggtcacct ctcgtcaggt cggccagtgc
      241 atgatcgccc tgaagctgtg tcgcgacatg aagaaaccgg gccgagataa cctcacggat
      301 atcgcagggt atgccggctg catggaactc atcgatgagg cgcagtcatg agcaacgccc
      361 gcatgaccgc cgaacaggcc cgcgaatatc tcgcgacctg ggccaagcat ggattcagtt
      421 gcgccgcaac agctcgcgcc accggcctat cgcggaccac ctggagtgac cgcatcagca
      481 ccgcgaagat ccgctgcccg gattccggca tcgtcccgac cgatgttcca gacggcatga
      541 agttcaccaa gaccaccgtg cagtacgatg gcgccggcaa cgtgatccag gagtggcgcc
      601 gcctgcacaa agacgccgag agcatggaag ccctggtcga agccctggcc gctgggctca
      661 agggcaagtg cccggcactg ccacgaccgg cgaagccagt caacaacaag cgcacgctgg
      721 tgttcccgat ctacgacccg cacttcggca agcatgcgtg ggaagctgag accggcgaga
      781 actacgacat caagatcgcc gagcgcgttc tggtcaacac cgcccgcatt gcgatggaac
      841 gcagcggacc tctggacgag gtggtcttca tccttggtgg cgactggttc catagtgaca
      901 cgcgcacgcc acagactgag cgcagcggac atgtgctcga cgtcgacacg agatccgagc
      961 gagtgcgcga cgtgaccatc aaggctatcg tggcggccat cgccatcttc tccgcggtcg
     1021 cgaacaaagt gcatgtcgtc tgcataccag gcaatcatga ccacgagtcg atgcactgga
     1081 ctgcgcgcat actcgaagcg gtctacgctg gccagaagca catcacggtc gatcgctcgc
     1141 cccggtcgcg tcgatacctg aagcgcggca agatgctgct cggtatcgat cacggcatga
     1201 tgaagatcaa cgagtacctg acgctcatgc cctgcgaggc ggccgatctc tgggccacca
     1261 ccaccgagcg cctgtggctg cttggtcaca tccatcagca gaaggtcatc cagaagcacg
     1321 gcgtgacggt cgagcacctg gagtccatca gcgccaccga tgcctggcac gccgagtctg
     1381 gctatgtcgg caacccacgt cgcacggtga cattcctcat cgatcccagc tatggcctcg
     1441 tgagccggag ctacatcact gttcaggagt ccatgcaatg acccccgtcg aactcaccca
     1501 ccacctccag cgccttcgcc gcagcgatca ctggtacaaa catcgcgctg ctgctgcggt
     1561 cgatctcaac tggtacgagg tcattccgag ccgttccgag aacagcgtgt atctcgtcag
     1621 gttctggctc aacaagccgg cgatcgatgg cagcggcaaa ctcgacagca gcaatgccac
     1681 gctgctccac tggttcgtct cgcacgatga tgacggcgcg ctgcatgatc atccgtggga
     1741 cttctcgaca accgtgttgg ctggcggcta catcgagacc acgccagcgc gcaagccagg
     1801 tcgacttgga cccggaacgt tcaaggccac gacggtgacc gcgggagcga cgctccagca
     1861 cgaggccgct gacctgcacg cgatcgccgg gctgcttggc gagacctgga ccctggtgac
     1921 taccggcccc cggatccgcg agtggggctt ccatccgccc ggacaggcct gggccgactg
     1981 gcggaccttc ctggccccga agcgtggcg
//
LOCUS       DATMTW010000022         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_236675, whole genome shotgun sequence.
ACCESSION   DATMTW010000022 DATMTW010000000
VERSION     DATMTW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236675"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1672)
                     /locus_tag="VN084_00425"
     CDS             complement(44..1672)
                     /locus_tag="VN084_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWT27945.1"
                     /translation="MAAHEQQILLSRLERLNAIGVALSAERNNKRLLEMILLGAKEIT
                     NADGGTLYTVTEDQQLKFEIMRTQTLDIAMGGTTGKDIPFPPLPLYTQDGSPNLNMVA
                     AYCVLNDRTVNIEDAYEAAGFDFSGTRKFDQNTGYRSKSFLTIPMKNHENEIIGVLQL
                     INAIDPDSGEIIAFSKANQQLVESLASQAAVALTNYNLIEGLKNLFEAFIELIADAID
                     EKSPYTGGHCRRVPELTMMLADAACRAERGPLADFRMTDKEIYELRIAAWLHDCGKVT
                     TPEHVMDKPTKLSTIYDRIHLVEQRFELLRSQAECDMLRRQLRALREGRPQDAERLQI
                     EFDAFCQQLQADRAFLQQANIGGEYMTPEHKQRVDEIATYQLESGGKQVPFLSEDEVY
                     NLKIERGTLTPEERTVINNHIVVTIHMLESLPYPKDLKRVPEYAGGHHERMDGKGYPR
                     GLRREEMSVPARVMGIADIFEALTSRDRPYKKAKTLSESLHILGKMKLDNHVDPDLFD
                     IFIREKIYQRYAEMFLEPEQIDKVDESAIPGYVP"
     gene            complement(1749..>2146)
                     /locus_tag="VN084_00430"
     CDS             complement(1749..>2146)
                     /locus_tag="VN084_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWT27946.1"
                     /translation="RPAAVKAAAAEAQKMQSTVAQPAMAPAAAAPSVAPPPPAASTEP
                     VGRIQVLSGPSTGKELVLNKALTTLGKPGVQVAVITKRPQGYFITHVEGQQHPVVNGQ
                     SVGAQAHGLRDHDVIELAGVKMEFYLAQP"
BASE COUNT          400 a          623 c          657 g          466 t
ORIGIN      
        1 aacaatatct tctgtttact ggtcatgcgg ccaagggtca cgttcagggc acatagccgg
       61 gaatggccga ttcatcgacc ttgtcgatct gctccggctc gaggaacatc tcggcatagc
      121 gctggtagat cttctcgcgg atgaagatat cgaagagatc agggtcgaca tggttatcca
      181 gcttcatctt gcccaggata tgcaacgact cggagagggt cttcgctttt ttgtaaggcc
      241 ggtcgcgcga ggtcagcgct tcgaagatat cggcgatgcc catcacccgg gccggcaccg
      301 acatctcctc gcgtcgcaag ccccgcggat agcctttgcc gtccatgcgt tcatggtggc
      361 cgcctgcgta ttccggtaca cgcttcaagt ccttgggata gggcagcgat tccagcatgt
      421 ggatggtgac gacgatatgg ttgttgatga ccgtgcgctc ttccggcgtc aaggtaccgc
      481 gctcgatctt gaggttgtag acctcgtcct cactaaggaa gggaacctgc ttgcctccgg
      541 attcgagctg gtaggtggcg atctcatcca cgcgctgctt gtgctcaggc gtcatgtatt
      601 caccaccgat gttggcctgc tgcaggaatg cacgatcggc ctgcagttgc tggcagaacg
      661 catcgaactc gatctgtagc cgctcggcat cctgaggccg cccttcacgc aaggcgcgca
      721 gttgccggcg caacatatcg cattccgcct ggctgcgcag cagctcgaaa cgctgttcga
      781 ccaagtggat acgatcatag atcgtcgaca gcttggtcgg cttgtccatg acatgttcag
      841 gcgtcgtcac cttgccgcaa tcatgcagcc aggctgcgat ccgtaattcg tagatctcct
      901 tgtccgtcat gcggaaatcg gcgagcggcc cgcgctcggc gcgacaggcg gcatccgcca
      961 gcatcatggt cagttccggt acgcgtcgac aatggccgcc ggtataagga gatttctcgt
     1021 cgatggcgtc ggcgatgagc tcgatgaaag cctcgaacag attcttcagg ccctcgatca
     1081 ggttgtaatt ggtgagtgcg accgctgcct gcgaagcaag cgattccacc agttgctggt
     1141 tggccttgga gaaggcgatg atctcgccgc tgtcggggtc gatggcgttg atcagctgca
     1201 acacgccgat gatttcgttc tcgtggttct tcatcgggat ggtgaggaag gacttggagc
     1261 ggtagccagt gttctggtcg aacttgcggg tgccggaaaa atcgaagccc gctgcctcat
     1321 aggcatcctc gatattcacc gtgcggtcat tgagcacgca ataggcggcg accatattga
     1381 ggttcgggct accgtcctgg gtataaagcg gcaagggcgg gaacgggata tccttaccgg
     1441 tggtgccccc catggcgatg tcgagggtct gggtgcgcat gatctcgaac ttgagctgct
     1501 ggtcttcggt aacggtatag agcgtgccgc cgtcggcgtt ggtgatctcc ttggcaccca
     1561 gcaggatcat ctccagcaaa cgcttgttgt tgcgctcggc cgagagcgcg actccgatgg
     1621 cattgagccg ctcgaggcga ctcagcaaga tctgctgctc atgcgccgcc atgaacgacc
     1681 ccgatcagcg acaacattga tgcatgcaag gacaaaagcg accccctacc acccttggtc
     1741 aaccctgatc agggctgggc caaatagaat tccatcttga cacccgccag ctcgatcacg
     1801 tcatgatcgc gcaagccatg ggcctgcgct cctacggatt gcccgttgac cacagggtgc
     1861 tgctgccctt cgacatgggt gatgaaatag ccttgcggcc gtttggtgat cacggcgacc
     1921 tgcacgcccg gtttgcccag tgtcgtcagg gccttgttca ggaccaactc cttgccggtg
     1981 ctaggaccgg acagcacctg gatacggcca acaggctcgg tcgacgccgc tggcggtggt
     2041 ggcgcgacag aaggcgcggc agcggcgggc gccatggccg gctgggcgac tgtgctctgc
     2101 atcttctgcg cttctgcggc agcggctttc acggcagccg gccgga
//
LOCUS       DATMTW010000023         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_505131, whole genome shotgun sequence.
ACCESSION   DATMTW010000023 DATMTW010000000
VERSION     DATMTW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505131"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..534
                     /locus_tag="VN084_00435"
     CDS             187..534
                     /locus_tag="VN084_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWT27947.1"
                     /translation="MDVMLKRAYEHPAKTDGKRILVDRLWPRGLAKVKARIDLWLKDV
                     APSTELRQWFGHDPEKWPEFQKRYRAELKNNPALSELQALSRQENISLIYAARDHLHN
                     EAVVLKQILDRNT"
     gene            583..1167
                     /locus_tag="VN084_00440"
     CDS             583..1167
                     /locus_tag="VN084_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWT27948.1"
                     /translation="MKTDLSSLLAEIRACSLCSEYLPHGPRPVLQVHPSARLLIASQA
                     PGRKVHATGIPFNDASGKRLRDWLGIGEETFYDPTQVAIVPMGFCYPGKGASGDLPPR
                     PECAPQWRDALLAQLENIQLTVVIGRYAQACHLPEAASLTEAVTSWGDESATVIALPH
                     PSPRNNLWLKRNPWFEAQAVPLLRARVRKALDGG"
     gene            complement(1222..1872)
                     /gene="lolA"
                     /locus_tag="VN084_00445"
     CDS             complement(1222..1872)
                     /gene="lolA"
                     /locus_tag="VN084_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HWT27949.1"
                     /translation="MKPVSALPSLLFSVALTLAGASFSAPAAASGIAQLRAFVADTHS
                     ARAGFAQVVMSKSGRKQESSGSMAFARPGKFRWSYEKPYQQLLVSDGNKLWSYDKDLE
                     QVTIKKFGDAMGTAIGGTPAALLAGDRDIDKRFTLSDQGEVDGVDIVDAVPKDKNSTF
                     ARVRIGFVDKLPKMMEIRDNFGQVTTLLFSHFERNPALPAGTFTFTPPKGVDVVGE"
BASE COUNT          397 a          592 c          607 g          365 t
ORIGIN      
        1 ctgagttttc agccgcacaa actgccggtc tgcgggagaa ggagctactg aaatgcgggt
       61 gccattcgtg ctgccaggcc ccacaattcg cttaagcgcg acggcggaac cggctgtttg
      121 ctcggaccgg ctgcttttgc accgcagcca cttatcccaa ggtcaagtcc cgcatgagag
      181 ggaatcatgg acgtcatgtt gaagcgagct tatgagcatc cagccaaaac cgatggcaag
      241 cggattcttg tcgatcgcct ctggccgcga ggattggcca aggtcaaagc caggatcgac
      301 ctctggctaa aagacgtggc gccgtccacc gagcttcgtc aatggtttgg acatgacccg
      361 gagaaatggc cggaattcca aaagcgatat cgcgctgagc tgaaaaacaa tcccgcattg
      421 tcggagctac aagcactttc gcgccaagaa aacattagcc tgatttacgc agccagagat
      481 catttacaca acgaggcggt cgtgctcaaa cagatcctgg atcgcaatac ctaacagcca
      541 ttcaagctgg tgtcggcttg atcaaatgcc cgtcgtaccg atttgaagac cgatctttcc
      601 agcctgctcg ccgagatccg cgcctgttcg ctgtgcagcg aatatctgcc gcacggaccg
      661 cgacccgtgc ttcaggtcca tccctcggca cgcctgctga tcgcaagcca ggcgccgggt
      721 cgcaaggttc acgcaacggg cattccgttc aatgatgcga gcggtaaaag attgcgcgac
      781 tggctcggta tcggcgaaga aacgttttac gatcctacac aggtggcgat cgtaccgatg
      841 ggattctgtt atcccggcaa gggcgcatct ggcgacttgc cgccacgccc tgaatgcgcg
      901 ccccaatgga gagatgcgct gctggcacag ctggagaaca ttcagctcac cgtggtcata
      961 ggccgctacg cacaggcctg tcaccttccc gaagcggcat cgctgacaga ggccgttacc
     1021 tcctggggag atgaaagcgc cacggtgatt gcattgccgc atccgagccc gcgcaacaac
     1081 ctatggctca agcgcaaccc gtggttcgag gcgcaggcgg tgccgctgtt gcgagcacgg
     1141 gtcaggaagg cactggacgg cggctagagc gcttccgcct caagcgctgc aggttaggtg
     1201 attatctcag tgccgactgg atcactcgcc gaccacatcc acgcccttgg gcggcgtgaa
     1261 ggtaaaggtg ccggccggca gcgccgggtt gcgctcgaag tgagagaaca gcaaggtcgt
     1321 gacctgaccg aaattgtcgc ggatctccat catcttgggc agcttgtcga cgaagccgat
     1381 gcgcacgcgc gcgaaggtgc tgttcttgtc cttgggcacg gcatcgacga tatcgacgcc
     1441 atccacctcg ccctggtcgc tcagcgtaaa acgcttgtcg atgtcgcggt ccccggccag
     1501 cagcgcggcc ggggtaccgc cgatggccgt acccatggcg tcgccgaact tcttgatggt
     1561 gacctgctcg agatccttgt catagctcca cagcttgttg ccgtcgctga ccagcagctg
     1621 ctgatagggc ttctcgtagc tccagcggaa cttgccggga cgggcgaagg ccatgctgcc
     1681 ggaagattcc tgcttgcggc cggacttgct catcaccacc tgcgcaaacc cggcgcgcgc
     1741 ggagtgggtg tcggcaacga aggcgcgcag ttgggcgatg ccgctggccg cggccggtgc
     1801 ggaaaagctt gcgccggcga gggtcagcgc gacggaaaac agcagggaag gcagtgcgga
     1861 gacgggtttc attgccacat tttgccagag cggcgcgcgc gccgctggcc cggatgtttc
     1921 aggagtttgc gcgcgcgcgg cgcagcgccg actaagcgcc g
//
LOCUS       DATMTW010000024         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_69124, whole genome shotgun sequence.
ACCESSION   DATMTW010000024 DATMTW010000000
VERSION     DATMTW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69124"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VN084_00450"
     CDS             complement(<1..357)
                     /locus_tag="VN084_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWT27950.1"
                     /translation="MAKQFRIAPSILSANFAKLVQEITDVITSGTDIVHFDVMDNHYV
                     PNLTIGPLVCEAIRPVTDAIIDVHLMVKPVDRIIPDFAKAGANIITFHPEASDHIDRS
                     LSLVREHGCKSGLVFNP"
     gene            477..1724
                     /gene="hemA"
                     /locus_tag="VN084_00455"
     CDS             477..1724
                     /gene="hemA"
                     /locus_tag="VN084_00455"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWT27951.1"
                     /translation="MHLFTVGVNHTTAPLSIREHVAFQNEQLGQALRDLTQRHKVQEA
                     AILSTCNRTELYCNTEDPQAALDWLAHYHHLNPQNIQPYTYLYPHKDAVKHAFRVASG
                     LDSMVLGEPQILGQMKQAVRIAEHAGTLGTLLHKLFQRTFAVAKEVRTSTDIGASSLS
                     MAAASVKLAQKIFGNLAGQHVLFIGAGEMIELCAEHFAVQKPASMTVANRSLERGTEL
                     AARFEGKAMLLADLPERLAEFDIVVTSTASQLPIVGLGMVERAIKHRKHRPMFMVDLA
                     VPRDIEPEAAELDDVFLYTVDDLAQVVQEGLGNRKEAAAEAEAIIALRVENFMHWLKN
                     RDAVPTIRALRDQAERLRRTELEKAQKLLARGEDPTAVLEALSNALTNKLLHGPSHAL
                     NNHGAQDHEQLDALMRQLYQLDP"
     gene            1824..2903
                     /gene="prfA"
                     /locus_tag="VN084_00460"
     CDS             1824..2903
                     /gene="prfA"
                     /locus_tag="VN084_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWT27952.1"
                     /translation="MKPSMLKKLATLSERLDELNRLLSTEDATANMDNFRKLTREHAE
                     ITPIVEQYRAYEQCAADLQEAQAMLADPEMKVFAQEEIDNAKDKQEAIGVELQKLLLP
                     KDPNDERNIFLEIRAGTGGDESALFAGDLFRMYSRYAERQRWKVEVVSANESELGGYK
                     EIIAKIEGFGAYSKLKFESGGHRVQRVPDTETQGRIHTSACTVAVLPEADEVSDVVIS
                     PADIRIDTFRASGAGGQHINKTDSAVRITHFPTGIVVECQDGRSQHANKAQAMQVLAA
                     RIKAKQVDEQQSKIASERKSLIGSGDRSERIRTYNYPQGRITDHRINLTLYKIDAITE
                     GDMDELINALLAEHQAELLATLGED"
     gene            2908..3807
                     /gene="prmC"
                     /locus_tag="VN084_00465"
     CDS             2908..3807
                     /gene="prmC"
                     /locus_tag="VN084_00465"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWT27953.1"
                     /translation="MPTIRTLLADARQALQQRGLPADEGRLEARLLLLQQLQVDHAWL
                     LAHEGDSLAADSHAGFQALLQRRLNGEPIAHILGQREFYGLMLKVTPDTLIPRPDTET
                     LVEAALAKIPDLSFVARQGCPAEYHDGIAPGFKVLDLGTGTGAIALAIASQRPHAEVL
                     AVDASIAALAVASENARSLHLRNVRFLQSDWFSALPDIRFDLVVSNPPYIADQDPHLS
                     QGDLRFEPLEALASGTDGLDDLRQIIAGAPRHLNRGGWLMLEHGYDQASRVGDLLQRA
                     GFSAIGQARDLAGIVRVSYGQLT"
BASE COUNT          842 a         1245 c         1118 g          640 t
ORIGIN      
        1 cgggttgaac accagtccgg acttgcagcc atgttcacgc accagggaca ggctgcggtc
       61 gatgtggtcg gaggcttccg ggtggaaagt gatgatattg gcaccggcct tggcgaaatc
      121 cgggatgata cggtcgaccg gcttcaccat caggtgcacg tcgatgatcg catcggtcac
      181 cggacggatc gcttcacata ccagcgggcc gatggtcagg ttcggaacgt aatggttgtc
      241 catcacgtca aagtggacga tatcggtgcc ggaggtgatg acatcggtga tctcttgaac
      301 cagcttggcg aagttggcgg agaggatact cggggcgatt cggaattgtt tagccatgat
      361 gatttgttct gcgtcagcta cgaaaagagt gcattttaac cgcaatctgc ctgtaaaaaa
      421 cagtctaggc gattgaaatt gttgcttaag cagggccgat acaggaaaat tacgggatgc
      481 atctattcac tgtcggcgtg aaccacacga ccgcaccgct ttccatccgt gagcatgtcg
      541 cttttcaaaa tgaacagctg ggacaagcct tgcgcgacct cacgcagcgc cacaaggtgc
      601 aggaagcggc tatcctctcg acctgcaacc gcaccgagct ctactgcaac accgaagacc
      661 cgcaggccgc gctcgactgg ctggcccact atcaccacct gaacccgcag aacatccagc
      721 catacaccta tctctacccg cacaaggatg ccgtcaagca tgccttccgt gtcgcttccg
      781 ggctggattc gatggtgctc ggcgaaccgc agatcctggg ccagatgaag caggctgtgc
      841 gtatcgccga acatgcaggg acattgggaa cgctactgca caagctgttc cagcgtacct
      901 tcgccgtcgc caaggaagtg cgcaccagca ccgatatcgg cgccagctca ctgtcgatgg
      961 cggccgcctc ggtcaagctg gcgcagaaga tcttcggcaa cctggccggt cagcacgtgc
     1021 tcttcatagg cgccggcgag atgatcgaac tgtgtgccga acatttcgcc gtacagaagc
     1081 cggcgtcgat gacggtggcc aaccgctcgc tggaacgtgg caccgagctc gccgcgcgtt
     1141 tcgagggcaa ggccatgttg ctggcggatc tgcccgagcg cctggcggag ttcgacatcg
     1201 tcgtcacctc cactgccagc cagctgccga tcgtcggcct aggcatggtg gaacgcgcga
     1261 tcaagcaccg caagcaccgg cccatgttca tggtcgacct ggctgtacca cgcgacatcg
     1321 aaccggaagc ggctgaactg gacgacgtgt tcctctatac cgtggacgat ctcgcacaag
     1381 tcgtacagga gggcttgggc aaccgcaagg aagccgctgc cgaggcagaa gccatcatcg
     1441 ccctgcgcgt cgagaacttc atgcactggc tcaagaaccg cgatgccgtg ccgaccatcc
     1501 gcgccctgcg cgaccaggcc gagcgcctgc gccgcaccga gctggaaaag gcccagaaac
     1561 tcctcgccag gggcgaagac cccacggccg tgctcgaggc actgagcaac gcgctcacca
     1621 acaaactgct gcatggcccc agccacgcac tcaacaacca cggcgcacaa gatcatgaac
     1681 agctcgacgc cctcatgcgc caactctacc agctggatcc atgaacccga ccagcacgca
     1741 tcgcgcgccg agcggatgaa cactggctac cacttgacgc gtaacgccga ccccctagcc
     1801 atcgcgcctg aaagaaattt gaaatgaaac cctcgatgtt gaaaaagctc gccaccctga
     1861 gcgagcgcct ggatgaactc aaccgcctgc tgagcacgga agacgccacg gcgaacatgg
     1921 acaattttcg caagctgacg cgcgaacatg ccgagatcac gcccatcgtc gagcaatacc
     1981 gtgcctatga acaatgcgcg gcggacctgc aggaagcgca agccatgctg gccgatccgg
     2041 agatgaaggt gttcgcgcag gaagagatcg acaacgccaa agacaagcag gaagccatcg
     2101 gcgtcgagtt gcagaaactg ctgctgccca aggaccccaa tgacgagcgc aatatcttcc
     2161 tcgagatccg ggccggtacc ggcggcgacg aatccgcact gttcgcgggc gatcttttcc
     2221 gcatgtactc ccgctacgcc gagcgccagc gctggaaggt ggaagtggtc tcggccaacg
     2281 aatccgagct cggcggctac aaggagatca tcgccaagat cgaggggttc ggtgcctact
     2341 ccaagctgaa gttcgagtcc ggcggacacc gtgtgcagcg cgtaccggat accgagaccc
     2401 agggccgcat ccacacctcg gcctgtaccg tggccgtatt gccggaagcc gacgaggtca
     2461 gcgacgtcgt catcagcccg gccgatatcc gcatcgatac cttccgcgcc tccggggccg
     2521 gcgggcaaca catcaacaag accgattccg ccgtgcgcat cacccacttc cccaccggca
     2581 tcgtcgtcga atgccaggat ggccgcagcc agcacgccaa caaggcgcag gccatgcagg
     2641 tgctggccgc acgcatcaag gccaagcagg tcgacgaaca gcaatccaag atcgccagcg
     2701 aacgcaagtc gctgatcggc tctggcgacc gctctgagcg tatccgtacc tacaattatc
     2761 cccagggtcg catcaccgac caccgcatca acctcacgct gtacaagatc gacgccatca
     2821 ccgagggcga catggacgag ctgatcaacg cgctactggc cgagcaccag gcggaattgc
     2881 tggcgacgct gggcgaagac tgagaccatg ccgaccatcc ggacgctgct cgcggatgca
     2941 cggcaagcct tgcaacagcg cggcctgcct gccgacgaag gtcggctcga agcccgcttg
     3001 ctgctgttac aacaactaca ggtcgaccat gcctggctgc tcgcacatga aggcgacagc
     3061 ctggccgccg attcccatgc agggttccag gcgctgctgc agcgccgctt gaacggcgaa
     3121 ccgatcgccc atatcctcgg ccagcgcgaa ttctacgggc ttatgctgaa agtcacgccg
     3181 gacacgctga tcccacggcc ggataccgaa accctggtcg aggcagcgtt agccaagata
     3241 ccggacctat ccttcgtagc caggcagggc tgccccgccg agtatcacga tggcattgca
     3301 ccaggattca aggtgctcga tctcggcacc ggcaccggcg cgatcgcact cgcgatcgct
     3361 agccagcgcc cgcacgccga agtcctggca gtcgacgcct cgatcgccgc gttggccgtc
     3421 gccagcgaaa acgcccgtag cctgcatctg cgcaatgtgc gcttcctgca aagcgactgg
     3481 ttttcggcct tgcccgatat cagattcgat ctcgtcgtca gtaacccgcc ttacatcgcg
     3541 gaccaggacc cgcatctcag tcagggcgac ctgcgcttcg agcctcttga ggcgctggct
     3601 tctggcacag acggcctcga cgacctgcgc caaatcatcg ctggcgcccc acgacatctc
     3661 aacaggggcg gctggctcat gctggaacat ggctacgatc aggcaagcag ggtcggtgat
     3721 ctgttgcagc gcgccggatt ttcggcaatc gggcaggcgc gtgacctggc aggtatcgtc
     3781 cgcgtcagct acggccagct gacctagctg gcatcaaagg aataaccggg taacgacggc
     3841 gagag
//
LOCUS       DATMTW010000025         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_170034, whole genome shotgun sequence.
ACCESSION   DATMTW010000025 DATMTW010000000
VERSION     DATMTW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170034"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VN084_00470"
     CDS             complement(<1..243)
                     /locus_tag="VN084_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27954.1"
                     /translation="MTAPPFLRLLAGLALLPAIAFADTNAAGDEITILWDAPAPAKHD
                     TNLSFSLTPIDPVPSYLPDDSEIAALENPAELSPEDD"
     gene            complement(344..1096)
                     /gene="gloB"
                     /locus_tag="VN084_00475"
     CDS             complement(344..1096)
                     /gene="gloB"
                     /locus_tag="VN084_00475"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HWT27955.1"
                     /translation="MLEILAIPAFKDNYIWLLRKGQHAVIVDPGDASVVIQALEQHHL
                     SLDAILITHHHSDHIDGVDNLLARHPCPVYAPLRERYAFPHTPVSENDRIVLDSLDLE
                     LKVIEVPGHTLGHVAYFGNGLLFCGDTLFGAGCGRLFEGTPAQMYSSLQKLAQLPPET
                     RVYCTHEYTEHNLRFALGLEPDNTALQQRALAASSLRQAGLPTLPSTIALERETNPFL
                     RCEQSTIQRAARIDSSDPVAVFTAIRESRNHY"
     gene            1138..1863
                     /locus_tag="VN084_00480"
     CDS             1138..1863
                     /locus_tag="VN084_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWT27956.1"
                     /translation="MIPIDRQQWLQGELGQYLCEREQEVFDRMVADVFGFNAVQVGMP
                     ELDLLRNCRIPFGFRAGGAQGDVDCTPEYLPLGEKCIDLVLLPHPLEFSQNPHQVLRE
                     VERVLVPEGHVVISGFNPLSLWGAKRACSKRVDYPWNGAFLSQPRIKDWLALLGFEVM
                     AWHMACYAPPLRSPTWRARFGFMDKADKNWWPIPGGVYFVIAKKRVLGMRLIKPNWNK
                     AKLKPGLLPAPTQKSEPQKKRHE"
     gene            1856..2323
                     /gene="rnhA"
                     /locus_tag="VN084_00485"
     CDS             1856..2323
                     /gene="rnhA"
                     /locus_tag="VN084_00485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HWT27957.1"
                     /translation="MNNPSEQAQAGWVVIHADGACKGNPGPGGWGAWLSHEGREKELY
                     GGEPQTTDNRMELTAVIRALEALKRPCKVRVYTDSVYVQKGITEWIHGWKSRNWRTSD
                     KKPVKNDDLWRELDTLAQQHEIEWHWVKGHAGHVGNERADALANRGVETLLIK"
     gene            2359..3066
                     /gene="dnaQ"
                     /locus_tag="VN084_00490"
     CDS             2359..3066
                     /gene="dnaQ"
                     /locus_tag="VN084_00490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWT27958.1"
                     /translation="MRQIFLDTETTGLYPAQGHRIIEIAGVEVINRQLTKNHFHVYLN
                     PEREIDPGAQEVHGITLEFLQDKPLFADIVDELLDFVAGSELVIHNAPFDVGFLNTEL
                     GRLGRSVIEESAAKIIDTLKLAKEMRPGQRNNLDALCRHFGIDNSRRTLHGALLDAEL
                     LADVYLAMTRGQNSLMIDMLEPVTAISELGPLMRETPLRVIGASEAELTAHQDYLTAL
                     AKGGACVWQQYEDKKPS"
     gene            3063..4157
                     /gene="rfbB"
                     /locus_tag="VN084_00495"
     CDS             3063..4157
                     /gene="rfbB"
                     /locus_tag="VN084_00495"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWT27959.1"
                     /translation="MTSNPPSTILVTGGAGFIGANFILAWRAAGLGRVINLDKLTYAG
                     NLQSLESLRDDAMHEFVHGDIGDRALVEQLLETHRPRAVINFAAESHVDRSIHGPEDF
                     IQTNVVGTFHLLEAVRAHWGNLQDDEKTNFRFLHVSTDEVYGSLGPDDPAFTETTPYA
                     PNSPYSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGAYQFPEKLIPLVIHNALAGKP
                     LPIYGDGSNVRDWLYVGDHCAAIRRVLEVGQIGETYNIGGCNEKRNLDVVHTLCDILD
                     RQQPKASGSYRDQITFVKDRPGHDRRYAIDAGKIGRELGWQPQETFETGIEKTVAWYL
                     ANQAWVANVTSGAYRDWVDSHYSEHSGATL"
     gene            4154..>4653
                     /locus_tag="VN084_00500"
     CDS             4154..>4653
                     /locus_tag="VN084_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWT27960.1"
                     /translation="MKGIILAGGSGTRLYPVTQVVSKQLLPVYDKPMIYYPLSTLMLA
                     GIREVLVISTPQDTPRFEQLLGDGAQWGMQISYAVQPSPDGLAQAFIIGSAFIGRDPC
                     ALVLGDNIFYGHELGQMAQTAAQREQGATVFAYPVHDPERYGVVEFDAAGKAISLEEK
                     PQQPKSR"
BASE COUNT         1000 a         1326 c         1405 g          922 t
ORIGIN      
        1 atcatcttcc ggcgatagct cggctggatt ctccagcgcg gcgatctcgc tgtcgtcagg
       61 cagataactg gggacggggt cgatcggggt caggctgaaa ctgaggttgg tgtcatgctt
      121 ggcgggtgca ggcgcatccc agaggatcgt gatctcgtcg ccggcggcat tggtgtcggc
      181 gaacgcgatc gcgggaagca aggcgagacc ggccagcagt ctcagaaatg ggggtgcggt
      241 catgcagttc tctgacggcc aaaaaattcc aacgatagta acgaacactc tgccgtgcag
      301 tcaagtaaaa gctaagcttt atatttacaa aatatcttaa tgattaataa tgatttcttg
      361 attccctgat ggcggtgaaa accgctacgg ggtctgatga gtcgatacgg gcggcgcgtt
      421 ggatggtcga ctgctcgcag cgcaggaagg gattggtctc gcgttcgagc gcaatggtgg
      481 agggcagggt cggcaatccg gcttgacgca ggctgctcgc tgccagcgct cgttgctgca
      541 gtgcggtgtt gtcgggttca aggccgaggg cgaagcgcag attatgttcg gtatactcat
      601 gcgtgcaata gactcgggtc tccggcggca attgcgcgag cttctgcagc gagctataca
      661 tctgggcggg tgtgccttcg aacagcctgc cgcagccagc gccgaacagt gtatcgccgc
      721 agaacagcag cccattgccg aagtaagcga catgccccag cgtatgacca ggcacctcga
      781 tcaccttcag ctccaggtcc aggctgtcga gcacgatgcg atcattctcg cttacgggtg
      841 tgtgcggaaa cgcatagcgc tcgcgcaacg gcgcgtagac aggacaaggg tgccgcgcca
      901 gcaagttgtc cacgccatcg atatggtctg aatggtgatg cgtgatgagg atggcgtcga
      961 gcgacaagtg gtgctgttcg agtgcctgga tgacaacgct agcgtcgccc gggtctacaa
     1021 tgacggcatg ctgacctttg cgcaacagcc agatgtagtt atccttgaaa gcgggtatgg
     1081 caagaatttc caacatgatg ttgtccagta taacggcgat ttgatgccgg ctgaaccatg
     1141 atccctatcg atcgacagca atggctgcag ggcgagctag ggcagtatct ctgcgagcgc
     1201 gagcaggaag tgttcgatcg tatggtggcc gatgtgttcg gcttcaatgc cgtgcaggtc
     1261 ggcatgccag agcttgacct gctgcgtaac tgccgcatcc ccttcggttt ccgtgcaggg
     1321 ggcgcacagg gggatgtgga ttgcacgcct gaatacctgc ctctgggtga gaaatgtatc
     1381 gatctcgtcc tgctgccaca tccgctcgaa ttcagccaga acccgcatca ggtcttgcgc
     1441 gaagtcgagc gcgtgctggt gccggaaggt catgtcgtga tcagcggctt caatcccctg
     1501 agtctgtggg gcgccaagcg ggcgtgttcc aagcgcgtgg attacccatg gaacggcgct
     1561 ttcctgagtc agccgcgtat caaggactgg ctggccttgc tcggatttga agtcatggcc
     1621 tggcatatgg catgctatgc gccgcctttg agaagcccga cctggcgagc acgattcggg
     1681 ttcatggaca aggccgacaa gaactggtgg ccgatccctg gcggggtgta tttcgtcatc
     1741 gccaaaaagc gggtgctcgg catgcgcttg atcaagccca actggaacaa ggccaagctc
     1801 aagcctgggt tgttgccggc gcctacccaa aaatcagaac cacagaaaaa acgacatgaa
     1861 taatccatca gaacaagcac aggccggctg ggtcgtgatc catgcggatg gcgcatgcaa
     1921 aggcaacccc gggcctggcg gctggggcgc ctggcttagc catgaggggc gggagaaaga
     1981 gctctacggc ggtgagccgc agaccaccga taaccgcatg gagctaacgg cggttatccg
     2041 cgcgttggaa gcgctaaaac gcccttgcaa ggtgcgtgtc tacacggatt ccgtctatgt
     2101 gcaaaaaggc atcacggaat ggatccatgg ctggaaatca cgcaactggc gaaccagcga
     2161 caaaaagccg gtcaagaacg atgatctctg gcgggagctc gacacgctcg cgcaacaaca
     2221 tgaaatcgag tggcattggg tcaaggggca tgccggtcat gtcggtaacg aacgcgctga
     2281 tgccttggcc aatcgaggtg tcgagacgct gctaatcaag taagatagcg gctcagaaaa
     2341 ccacgggacc gatcgctgat gagacagata ttcctcgaca cggaaacgac cggcctctac
     2401 ccggcgcaag gccaccgtat catcgagatc gccggtgtcg aggtcatcaa ccgccagctc
     2461 accaagaacc acttccatgt ctacctcaat ccggagcgcg agatcgatcc cggcgcacag
     2521 gaggtgcatg gcatcacgct ggaattcctg caggacaagc ccttgtttgc ggacatcgtc
     2581 gacgagcttc tcgatttcgt cgccgggtct gagctggtga tccataacgc acctttcgac
     2641 gttggattcc tcaacactga acttgggcgt ctcgggcgtt ccgtgatcga ggagtcggca
     2701 gccaagatca tcgataccct gaagctcgcc aaggagatgc gtccgggcca gcgtaacaac
     2761 ctcgatgcgc tgtgccgtca ttttggcatc gacaactcca ggcgcacctt gcatggcgca
     2821 ttgctggacg cggaactgct ggcagacgtc tatctggcca tgacacgcgg tcagaatagc
     2881 ctgatgatcg acatgctgga acccgtcacg gcgatcagtg agcttgggcc gctcatgcgc
     2941 gaaacgccac tgcgtgtgat aggcgcgagt gaagcggagc tgacagccca tcaagactac
     3001 ctcacagcgc ttgccaaagg cggcgcgtgt gtctggcaac aatacgaaga caagaaacct
     3061 tcatgacatc gaatccacca tccaccattc tcgtcactgg cggcgccggg ttcatcggcg
     3121 ccaatttcat cctcgcgtgg cgggctgccg gccttggtcg cgtcatcaac ctcgacaagt
     3181 tgacctatgc cggcaatctg caaagcctgg aatcgctgcg tgacgatgcc atgcatgagt
     3241 tcgtgcatgg cgatatcggt gatcgggcgc tggtcgagca attgctggaa acgcatcgtc
     3301 cgcgcgcagt catcaacttc gccgcggaga gtcatgtcga tcggtccatc catggccccg
     3361 aggatttcat ccagaccaat gtcgtcggta cttttcatct gctggaagcc gttcgcgccc
     3421 attggggcaa cctgcaagat gacgaaaaga cgaatttccg cttcctgcac gtctccactg
     3481 acgaggtcta tggctcactt gggccggacg atcctgcgtt caccgaaacc acgccttatg
     3541 ccccgaacag cccatactcg gcctccaagg ctgcttccga ccatctggtc agggcctacc
     3601 atcacaccta cggcctgccg gtgctgacca cgaactgctc aaacaactac ggggcttatc
     3661 aatttcccga aaagctgatt ccactggtca tccataatgc gttggccggc aagccgctgc
     3721 cgatctacgg cgacggcagc aacgtccgtg actggctcta tgtcggcgac cactgcgcgg
     3781 ctatccgtcg cgtgctcgaa gtcgggcaga tcggcgagac ctataacatc ggtggctgta
     3841 acgagaaacg taatctcgac gtggtacata cgctatgcga tatcctcgac cgccagcagc
     3901 ccaaggcatc aggcagttac cgggaccaga tcactttcgt caaggatcga cctggccatg
     3961 accggcgtta cgccatcgac gctggcaaga tcggtcgcga acttggctgg cagccgcagg
     4021 agaccttcga gaccggcatc gagaagaccg tagcctggta tctggcgaat caggcctggg
     4081 tggccaacgt caccagtggc gcctatcgcg actgggtcga cagccattac agcgagcaca
     4141 gcggagcaac actatgaaag gcatcatcct cgccggtggt tccggcacgc gcctctatcc
     4201 cgtgacgcaa gtggtttcca agcaactgct gccggtctat gacaagccga tgatctacta
     4261 tcccttgtcg acgctgatgc tggccggcat ccgtgaggtg ctggtcatct ccacaccgca
     4321 ggatacgccg cgcttcgagc agttgctggg tgatggcgcg caatggggca tgcagatcag
     4381 ttatgcggtg cagccgtcgc cggatggcct ggcgcaggcg ttcatcatcg gcagtgcgtt
     4441 cattggtcgc gatccctgcg ccctggtgct tggcgacaat atcttctatg gccatgagct
     4501 cggccagatg gcccagacag cagcgcagcg cgagcagggc gctaccgtgt tcgcctatcc
     4561 ggtgcatgac ccggagcgct atggcgtggt cgagttcgat gccgccggca aggcaatcag
     4621 tttggaagag aaaccgcagc aacccaagtc gcg
//
LOCUS       DATMTW010000026         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_304233, whole genome shotgun sequence.
ACCESSION   DATMTW010000026 DATMTW010000000
VERSION     DATMTW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304233"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="gpmI"
                     /locus_tag="VN084_00505"
     CDS             <1..1237
                     /gene="gpmI"
                     /locus_tag="VN084_00505"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWT27961.1"
                     /translation="LHIFGLLSDGGVHSHEDHIHAMAEMAVRQGLQKVYVHAFLDGRD
                     TPPVSAAAYIEKLEQKLATLGAGKIISICGRFYAMDRDKRWPRVEAAYDLITEGISEY
                     QAPTASAGLQAAYARGENDEFVKPTAIVQPGEKPLHLEDGDVVVYMNFRSDRARQLNH
                     ALVAQTFEGFHRRHVRKLAGYFSLTMHDKNETAIVPIFPPFEVRNTFGEYIAGKGLTQ
                     LRIAETEKYPHVTFFFNGGEEQVFAGEDRILVPSPQVATYDLKPEMSAFEVTAELEAA
                     ILSKKYDAIICNFANCDMVGHTGDLQAAIRAVEAVDSCIGRVVAAMQSIGGEVIITAD
                     HGNVEMMMDNVNHQPHTQHTTNLVPMLYIGRPARLAETGALSDLAPTLLKMMGLPQPA
                     EMTGKPLVEFAQATAAASA"
     gene            1242..>1550
                     /locus_tag="VN084_00510"
     CDS             1242..>1550
                     /locus_tag="VN084_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HWT27962.1"
                     /translation="MQRAIARLTLLASLLLGLLAPGVAWAGAKAEQSKEDLNRLHERI
                     EALKKELDSTKEAHKDATDELKQSEKAISETNRKLYELRKQHQQNRNTLASLQQQKLE
                     T"
BASE COUNT          347 a          511 c          451 g          241 t
ORIGIN      
        1 cctgcatatc tttggcctgc tctccgacgg cggcgtgcac agtcacgagg accacatcca
       61 cgccatggcc gaaatggcgg tgcgccaggg actgcaaaag gtctacgtcc atgctttcct
      121 ggatggccgc gatacgcctc ccgtgagcgc cgccgcctac atcgagaagc ttgaacagaa
      181 gctggcgaca ctcggcgccg gcaagatcat ctccatctgc ggccgcttct acgccatgga
      241 ccgcgacaag cgctggccgc gggtcgaagc cgcctacgac ctcatcaccg aaggtatcag
      301 cgaatatcag gcgcccaccg caagcgccgg cttgcaggcg gcctatgcgc gcggcgagaa
      361 cgacgagttc gtcaagccga ccgctatcgt gcagccgggc gaaaagccgc tgcatctgga
      421 agacggcgat gtcgtggtct acatgaattt ccgctccgac cgcgcgcgcc aactcaacca
      481 cgcgctggtg gcgcagacct tcgagggttt ccatcgccgt catgtacgca agctggccgg
      541 ctatttttcg ctgaccatgc atgacaagaa cgagactgcg atcgtgccga tcttccctcc
      601 attcgaggta cgcaacacct ttggcgaata catcgccggc aagggcctga cgcaactgcg
      661 catcgccgaa accgagaaat acccgcacgt cacgttcttc ttcaacggtg gcgaagaaca
      721 ggtcttcgcc ggcgaagacc gcatcctggt gccctcgccg caggtcgcca cctatgacct
      781 caagccggag atgagtgcgt tcgaggtcac ggcagaactc gaggccgcga tcctcagcaa
      841 gaaatacgac gccatcatct gcaatttcgc caactgcgac atggtcggcc ataccggcga
      901 cctgcaggcg gcgatcaggg ccgtggaagc ggtggatagc tgcatcggtc gcgtggtcgc
      961 ggccatgcaa agcatcggcg gcgaagtcat catcaccgcc gatcacggca acgtcgagat
     1021 gatgatggac aacgtgaacc accagccgca cacgcagcac accaccaatc tggtgcccat
     1081 gctctacatc ggccggccgg ccaggttggc tgaaaccggc gcgctgtccg atcttgcccc
     1141 caccctgctg aagatgatgg gcctgcccca accagcggag atgaccggca agccgctggt
     1201 cgaattcgcg caggccacgg ccgctgccag cgcctgagcc gatgcagcga gcgatcgccc
     1261 gactgaccct gctcgccagc ctgctccttg gccttctcgc tccaggcgtt gcctgggcgg
     1321 gtgcgaaggc agagcaatcg aaggaagacc ttaaccgcct gcatgagcgt atcgaggcac
     1381 tgaagaagga actcgacagc acgaaggaag cgcacaagga tgcgaccgac gaactgaaac
     1441 agagcgaaaa ggccatcagc gagaccaatc gcaagctcta tgagctgcgc aagcaacatc
     1501 agcagaaccg caataccctg gccagcctgc agcagcaaaa actggagacc
//
LOCUS       DATMTW010000027         7455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_170363, whole genome shotgun sequence.
ACCESSION   DATMTW010000027 DATMTW010000000
VERSION     DATMTW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170363"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VN084_00515"
     CDS             <1..1149
                     /locus_tag="VN084_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWT27963.1"
                     /translation="LSVESSKPCTTQEDLSLAYTPGVAEPVREIHRDPANAYRYTNKG
                     NLIAVITDGTAVLGLGDVGALAGKPVMEGKGVLFKRFAGIDVFDIEVKAPSVEAFIET
                     VVNIAPTFGGINLEDIAAPHCFRIEKELRERLDIPVFHDDQHGTAVIVAAGLINALEL
                     QGKAIDQVKIVFLGAGAAGCACARLLKVMGATDITMVDRTGVLHSDRDDLHDMNCDLA
                     RKTQARTLEEVMPDADVFIGVSAANAMPAELLKQMAPRPIVFALANPDPEILPSAAHA
                     VRDDLIMATGRSDFPNQVNNVLGFPFLFRGALDARAKQITIAMQVAAARALAELARLP
                     VPQAVLDAYHLDALSFGPEYIIPKPFDPRLLEHVAGAVARAVRPDERR"
     gene            1153..1536
                     /gene="sdhC"
                     /locus_tag="VN084_00520"
     CDS             1153..1536
                     /gene="sdhC"
                     /locus_tag="VN084_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWT27964.1"
                     /translation="MHHDSRHRPKNLKLASIRLPIPALVSILHRATGALIFLLLPAFL
                     WTFQRSLSSPEGYQEVMALAQHQLVKLALLGVLWAFLHHFLAGIRHLTLDMRIGLEIR
                     QARRSSKLVLIGGLLLTLLIGACWW"
     gene            1533..1886
                     /gene="sdhD"
                     /locus_tag="VN084_00525"
     CDS             1533..1886
                     /gene="sdhD"
                     /locus_tag="VN084_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWT27965.1"
                     /translation="MTRLLRAACQCMHHASYEWLLQRFTAVVMAIYTLLIVGVLLVCQ
                     PSGYQAWQALFGATWLKIATLLFLISLFIHAWQGVRDIIVDYIYPHAIGKVLNVAVTI
                     ALIGYAVWSVRILWK"
     gene            1909..3663
                     /gene="sdhA"
                     /locus_tag="VN084_00530"
     CDS             1909..3663
                     /gene="sdhA"
                     /locus_tag="VN084_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWT27966.1"
                     /translation="MSLPSQSFDALIVGGGGAGLRAALQLSESGLNVAVISKVFPTRS
                     HTVAAQGGIAASLGNVGEDNWLWHMYDTIKGSDYLGDQDAIEFMCRNAPKAIYELEHF
                     GMPFDRMESGKIYQRPFGGMTKEFGASPISRTCAAADRTGHAMLHTLYQRNVKCNTNF
                     FIEWLALDLIRDSEGVVHGITAMKMETGEVSILQAKTTLFATGGAGRIFQASTNAFIN
                     TGDGLGMAARAGVPLQDMEFWQFHPTGVAGAGVLITEGVRGEGGYLVNSEGERFMERY
                     APSAKDLASRDVVSRAIATEIKQGRGVGKDKDHVLLKLDHLGEEIIKHKLPGIREIAK
                     NFADVDPVKDPIPVVPTAHYMMGGIPTNLDGQVVQNAAGQVVEGFYAVGECACVSVHG
                     ANRLGSNSLLDLMVFGRAAGDHMIDYVRNNPHHKPLPKDAGEETLARLARLDGQKDGE
                     SVTEAGDAMRRAMQVHCGVFRFPDLLESGVGKIRAVQARIARTGIKDKSKVFNTARIE
                     ALELDNLIEVAVATMTAAEARKESRGAHCREDHPQRDDEQWLKHSLYYSHDARLDYRP
                     VRMQPLTVESFPLKARVY"
     gene            3720..4481
                     /locus_tag="VN084_00535"
     CDS             3720..4481
                     /locus_tag="VN084_00535"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWT27967.1"
                     /translation="MRFSIYRFNPDVDLEPYMQDYELALQPNDRMLLDALLRIKEMDD
                     TLGMRKSCREGVCGSDAMNINGKNGLACVTKLADLSEPVVVRPMPGLPVVRDLIVDMT
                     QFFDNYHSVQPYLVNDDPVPETERLQSPEERAKLDGLYECILCGACTTSCPSFWWNPD
                     KFVGPAGLLQAYRFMADSRDQALDERLDNLEDPYRLYKCHNIMNCVDVCPKGLSPAKA
                     ISKIKELKMKGLKAGGLKAPAAGKPGRQISIKRVP"
     gene            4478..4729
                     /locus_tag="VN084_00540"
     CDS             4478..4729
                     /locus_tag="VN084_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HWT27968.1"
                     /translation="MSIEAGRMLWRCRRGLLELDIVLRVFVEQSYASLTEDEAEAFIR
                     LLAYEDNDLWDLVSGRMTLEDAAQVRVLETLKKHRLCLG"
     gene            4791..5927
                     /locus_tag="VN084_00545"
     CDS             4791..5927
                     /locus_tag="VN084_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWT27969.1"
                     /translation="MEHHYHNGEYCHVFNPSVLGDLWPGIQLYYPPVKYAPSLGIYED
                     LQQASQRMKKHAHNTNAHTLIFDLEDGCRQKEMSRTLLRQELPLLREKHPRVTVAIRV
                     NPFRTDEYELDMDMVRDMADYIDVIMLSKAGEAYGAPEVRDLSNLLVNLNSKITIQPI
                     IEHPKSLKIAPDLMQYSTVRHVVFGIHDFSKAMGISITPQNWTEELFYFLNQIMFEAR
                     IAGKGVIGGVETLIGSSSMPEKFVEPHDVRRWLDLHGENESRIVYKHAVQEAAMGLTG
                     KQVIHPAHIHLCKVAYVPSPTEIRQKINVLKAAIEADALLGGAIKFEGEMLDPPMFGK
                     ALQTLLRAHALRALSEEDTAFAITVLQQLPAQVIRQNWPYGVIL"
     gene            6084..>7455
                     /locus_tag="VN084_00550"
     CDS             6084..>7455
                     /locus_tag="VN084_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWT27970.1"
                     /translation="MITKTALKPFEPWVWQVPEYFNIGVACTDVHLGKPAEQRLAMIV
                     EDDERGTSEITFGQLAERTSRFAQLLHNLKINAGDRVLIRLPNSLDYPIAFLGAMKRG
                     AISVPTSTLLTAEEVLYLAKDSAATVLVTDKAMWRDLHDKLQGCANLKHVLLTGPGEV
                     IDVAGLQVRDLAQALDAIGSWGPAHRTRADDPAYLVYTSGTTGYPKGVLHAHRALIGR
                     LPAARYWFDFSDDGDRIMHSGKFNWTYVLGTGLMDPLSMGKTVIVFEGKNDANTWPRL
                     IAKHNATIFIGVPTIYRQVIQKSTFSKVDVPSLRHCMSAGEHLSDEVLMQWRERFGMD
                     IYEAVGMSEFSYYLCQTKSRPIRPGSAGFPQPGHDIRLLDPETLKPVKPGEEGMICVP
                     ESDPGLFLEYWQLPEETAKLRHDGWFFTGDYARYDEDGYIWFLGRKDDIIKSFGYRVS
                     PYEIERV"
BASE COUNT         1490 a         2271 c         2294 g         1400 t
ORIGIN      
        1 ctctcggtcg agtcgagcaa accctgcacc acacaggaag atctatccct ggcctatacg
       61 ccgggtgtgg cggagccagt gcgcgagatc catcgcgatc ccgccaatgc ctaccgctat
      121 accaacaagg gtaacctgat cgcggtgatc accgacggca cagccgtgct gggcctgggc
      181 gatgtcggcg cgcttgccgg caagccggtg atggaaggca agggcgtgct gttcaagcgc
      241 ttcgccggga tcgacgtgtt cgatatcgag gtcaaggcgc catcggtcga ggccttcatc
      301 gagaccgtgg tcaatatcgc gccgaccttt ggtggcatca acctggaaga catcgccgcg
      361 ccgcattgct tccgcattga gaaggagttg cgcgagcgcc tggatatccc cgtgttccac
      421 gacgaccagc acggcacggc cgtgatcgtc gcggctggcc tcatcaacgc attggagtta
      481 cagggcaagg ctatcgacca ggtgaagatc gtgttccttg gggccggcgc cgccggctgc
      541 gcctgtgcac ggctactgaa ggtgatgggt gcgaccgata tcaccatggt ggaccgtaca
      601 ggtgtgttgc acagcgaccg cgacgacctg catgacatga actgcgacct ggcacgaaag
      661 acccaggcca gaacgctgga agaagtgatg ccggatgcgg atgtattcat cggcgtctcg
      721 gccgccaacg ccatgcctgc cgaattgttg aaacaaatgg cgccccggcc catcgtcttc
      781 gcgctggcga atccggaccc ggagatcctg ccgagtgccg cccatgccgt gcgcgacgat
      841 ctcatcatgg ccaccggccg ttccgatttc ccgaaccagg tcaataacgt gctggggttc
      901 cccttcctgt tccgcggtgc gctggatgct cgcgcgaaac agatcaccat cgccatgcaa
      961 gtggcggcag cgcgtgcgct ggccgagctg gcacgactgc cggtgccgca agcggtgctc
     1021 gacgcctacc atctggatgc gctgagcttt ggccctgaat acatcatccc caagcctttc
     1081 gatccacgcc tgctcgagca tgtcgcgggt gcggtggcga gggcagtgcg tccggacgag
     1141 cgcaggtgaa gcatgcatca cgatagtcga catcgcccca agaacctcaa gctggcgagc
     1201 atccgcctgc cgatccccgc gctggtatcc atcctgcacc gggcgacggg cgcgctgatc
     1261 ttcctgctgt tgcccgcttt tctgtggacg ttccagcgat ccctgtcgtc gcccgaggga
     1321 tatcaagagg tcatggcgct ggcacagcat cagctggtca aactggcgct gttgggcgtg
     1381 ctctgggcct ttctgcatca cttcctagcc ggtatccgcc acctgacgct ggatatgcgc
     1441 atcggcctgg agatccgcca ggcccggcgc agcagcaagc tggtgctgat cggcggccta
     1501 ttgctgacct tgttgatcgg agcatgctgg tggtgacacg actactccgt gcagcctgcc
     1561 agtgcatgca ccacgcctcc tacgaatggc tgttgcagcg attcaccgca gtggtgatgg
     1621 cgatctacac cttgctgatc gtgggtgtgc tgctggtatg ccagccttcg ggctatcagg
     1681 catggcaggc gctgtttggc gcgacctggc tcaagatcgc gacgcttttg ttcctgatca
     1741 gcctgttcat ccatgcatgg cagggggtac gtgacatcat cgtcgattac atctacccgc
     1801 atgccatcgg caaggtcttg aatgtcgcgg tgaccatcgc cctcatcggc tatgccgtgt
     1861 ggtcggtacg gatcctctgg aaataattgt catgaaagct agtatctgat gagtttgccc
     1921 agtcaatctt tcgatgccct gatcgtcggc ggcggcggtg ccggtttgcg tgccgcgctg
     1981 cagttgtcgg agtccggcct caatgtcgcg gtcatctcca aggtgttccc cacgcgctcg
     2041 cataccgttg ccgcgcaggg cggtatcgcc gcttcgctcg gtaatgtcgg cgaggacaac
     2101 tggctctggc atatgtacga caccatcaag ggttccgatt acctgggcga ccaggacgcc
     2161 atcgaattca tgtgccgcaa tgcgccgaag gcgatctatg agctggaaca tttcggcatg
     2221 ccgttcgatc gcatggaaag cggcaagatc taccagcgcc cattcggcgg catgaccaag
     2281 gagttcggcg cgtcgccgat ctcgcgtacc tgcgccgcgg cggaccgcac cggccacgcc
     2341 atgctgcaca cgctctacca gcgcaacgtc aagtgcaaca ccaatttctt catcgaatgg
     2401 ctggcgcttg acctcatccg cgatagcgag ggcgtggtgc acggcatcac cgccatgaag
     2461 atggagaccg gcgaggtcag catcctgcag gccaagacca cgctgttcgc cacgggcggc
     2521 gctgggcgca tcttccaggc cagcaccaac gccttcatca ataccggcga tggcctcggc
     2581 atggccgcac gtgccggcgt acccttgcag gacatggaat tctggcagtt ccacccgacc
     2641 ggcgtcgccg gggccggcgt gctgatcacc gaaggggtgc gtggcgaggg tggctacctg
     2701 gtgaattcgg aaggcgagcg cttcatggaa cgctatgcgc ccagcgctaa ggatctcgcc
     2761 agccgcgacg tggtgtcgcg cgccatcgcc accgagatca agcaaggccg cggtgtcggc
     2821 aaggacaagg accacgtgct gctcaagctc gaccatctgg gcgaggagat catcaagcac
     2881 aagttgccag gcatccgcga gatcgccaag aacttcgccg atgtcgatcc ggtgaaggat
     2941 ccgatcccgg tcgtgccgac cgcgcattac atgatgggtg gtattcccac gaatcttgac
     3001 ggccaggtgg tgcagaacgc ggcgggacaa gtcgttgaag gcttctacgc ggtcggcgaa
     3061 tgcgcctgcg tctcggtgca tggcgccaac cgtctgggtt cgaattcgct gctcgacctc
     3121 atggtgttcg ggcgcgcggc gggtgaccac atgatcgatt acgtgcgcaa caatccgcat
     3181 cacaagccct tgccgaagga cgccggtgag gagacgctgg cgcggttggc ccggctcgat
     3241 gggcagaagg acggcgagag cgtgaccgag gctggcgatg ccatgcgtcg cgccatgcag
     3301 gtccattgcg gcgtgttccg tttccctgat ctgctggaat ccggtgtcgg caagatccgt
     3361 gcggtccagg cacgcatcgc tcgcaccggg atcaaggaca agagcaaggt gttcaacacc
     3421 gcgcgcatcg aagcgctgga gctggacaac ctgatcgaag tcgccgtcgc caccatgacc
     3481 gctgccgaag ctcgcaagga gagccgcggc gcgcattgcc gcgaggacca tccacagcgt
     3541 gacgacgaac aatggctcaa gcattcgctg tattacagcc atgacgcgcg gttggattac
     3601 cggccggtgc gcatgcaacc gctgacggtt gaatcgttcc cgctcaaggc acgtgtttac
     3661 tgatatgacc gcgtttactg aaatcaccaa gatgaaatca ataagatgag atcaacaaga
     3721 tgagattctc gatctatcgc ttcaacccgg atgtcgacct cgagccgtac atgcaggatt
     3781 acgagctcgc gctgcagccg aacgaccgga tgctgctcga cgcgctgttg cgcatcaagg
     3841 agatggacga cacgctgggc atgcgcaaat cctgccgcga gggcgtctgc ggttcggatg
     3901 ccatgaacat caacggcaag aacggcctgg cctgcgtcac caagctcgcc gatctcagcg
     3961 aacccgtggt ggtgcgcccg atgccaggcc tgccggtggt gcgcgacctg atcgtcgaca
     4021 tgacccagtt cttcgacaac tatcattcgg tgcagcccta cctggtcaac gatgaccccg
     4081 tacccgagac cgagcgcctg caatcgccgg aagaacgcgc caagctcgat ggcttgtacg
     4141 agtgcatcct gtgcggcgcc tgcacgacct cgtgtccctc gttctggtgg aacccggaca
     4201 agttcgtcgg ccccgcgggc ctattgcagg cttaccgctt catggccgac agccgcgacc
     4261 aggcgctgga cgagcgcctc gataatctgg aggacccata ccgcctctac aaatgccaca
     4321 acatcatgaa ttgcgtcgac gtctgcccca agggcctgag cccggccaaa gccatcagca
     4381 agatcaagga gttgaagatg aaaggtctca aagccggcgg gctgaaggcg cctgcggcag
     4441 gcaagcccgg caggcagatc agcatcaaga gagtgccgtg agtatcgaag ccggacgcat
     4501 gctatggcgt tgtcgcagag gcttgctgga gctggatatc gtgctgcgcg tcttcgtcga
     4561 gcagagctac gcgtcgctga ctgaagacga ggccgaagcc ttcatccgcc tgctggcgta
     4621 tgaggataac gatctctggg atctggtcag cggcaggatg acgttggagg acgccgcgca
     4681 ggtgcgcgtg ctggagacct tgaagaaaca caggctgtgt ctgggctgat tagcctggat
     4741 aaacgaacga caggaacagg acggaaacca tgaacaagcc gactgcgaca atggaacacc
     4801 actaccacaa cggggaatac tgccacgtgt tcaaccccag tgtgctgggc gatctctggc
     4861 cgggcatcca gctctattac ccgccggtga aatacgcgcc ctcgctcggc atctacgagg
     4921 acctgcagca ggcgtcgcag cgcatgaaga agcatgcgca caacaccaat gcccatacgc
     4981 tgatcttcga cctggaggac ggctgccgtc agaaggagat gagccgcacg ctgttgcgcc
     5041 aggagttgcc gttgctacgg gaaaagcatc cgagggtgac ggtcgccatc cgcgtcaatc
     5101 ccttccgtac cgatgaatac gagctcgaca tggacatggt gcgcgacatg gccgactaca
     5161 tcgacgtcat catgctgtcg aaggcgggcg aggcctatgg cgcgccggaa gtgcgcgacc
     5221 tgtcgaacct gctggtgaat ctcaacagca agatcaccat ccagcccatt atcgagcatc
     5281 ctaagtcgct caagatcgcg ccggacctca tgcagtattc caccgtgcgc cacgtggtgt
     5341 tcggcatcca tgacttctcc aaggcgatgg gcatcagcat cacgccgcag aactggacgg
     5401 aagagctgtt ctatttcctc aaccagatca tgttcgaggc gcgtatcgct ggtaagggcg
     5461 tcatcggcgg ggtcgagacc ctgatcggct ccagcagcat gccggagaag ttcgtcgaac
     5521 cgcatgacgt gcgccgctgg ctcgacctgc atggcgagaa cgaatctcgc atcgtctaca
     5581 agcatgcggt gcaggaagct gccatgggcc tgaccggcaa gcaggtcatc catccggcgc
     5641 acatccacct gtgcaaggtg gcctatgtgc cgtcgccgac cgagatccgg cagaagatca
     5701 acgtactgaa agcggcgatc gaggccgatg ccttgctcgg cggcgccatc aagttcgagg
     5761 gggagatgtt ggacccgccg atgttcggca aggccttgca gaccctgttg cgcgcccatg
     5821 ccctgcgggc gttgagcgaa gaggatacgg cgttcgccat cacggtgctg cagcagttgc
     5881 cggcacaggt gatacgccag aattggccat acggcgtcat tctctaaatg ttgatgagag
     5941 aaaaagcctg gacgttgttg cgcttgcttg ccgtacggat atgtactgtc tacgcaagcg
     6001 cgcctagtcc agacctgttt tctcatcaac cagaaaattg agcggactaa aagtccagga
     6061 gaagcaagat gggtgcagag caaatgatca cgaagacagc gttgaagcct tttgagccgt
     6121 gggtctggca ggtgccggaa tatttcaata tcggcgtcgc ctgcaccgac gtgcatctcg
     6181 gcaagccagc cgagcagcgc ctggcgatga tcgtcgagga tgacgagcgc ggcacctccg
     6241 agatcacctt cggccagctg gcggagcgga ccagccgttt cgcccagttg ctgcataacc
     6301 tcaagatcaa tgccggcgat cgtgtgctga tccgcctgcc gaattcgctc gattacccga
     6361 tcgcattcct cggtgccatg aagcgcggtg ccatctcggt gccgacctcg acgctgctca
     6421 cggcggaaga ggtgctctat ctcgccaagg attcagccgc cacggtgctg gtgaccgaca
     6481 aggctatgtg gcgcgacctg cacgacaagc tgcagggctg cgccaacctc aagcacgtgt
     6541 tgctcacggg accaggcgaa gtcatcgacg ttgcaggtct gcaggtgcgt gatctggcgc
     6601 aggcgctcga cgccatcggg agctggggtc cggcgcatcg tacccgggcc gacgatcctg
     6661 cctacctggt ctatacctcg ggcactaccg gttatcccaa gggcgtgctg catgcccatc
     6721 gcgcactgat cggccgcctg ccggcagccc gttactggtt cgatttcagt gacgatggcg
     6781 accgcatcat gcattccggc aagttcaact ggacctatgt gctgggtact ggcctcatgg
     6841 acccgctgtc catgggcaag accgtcatcg tcttcgaggg caagaacgac gccaatacct
     6901 ggccgcgcct gatcgccaag cacaacgcca ccatcttcat cggcgtgccg accatctatc
     6961 gccaggtcat ccagaagagc accttcagca aagtggacgt gcccagcctg cggcattgca
     7021 tgagcgcggg cgaacacctg tcggacgaag tgctgatgca atggcgcgag cgtttcggca
     7081 tggatatcta cgaagcggtc ggcatgtccg agttctcgta ttacctgtgc cagaccaagt
     7141 cgcggccgat ccggccgggc tctgccgggt tcccgcaacc cgggcatgac atcaggctgc
     7201 tcgacccgga gacgctgaaa cctgtgaagc caggcgagga gggcatgatc tgcgtgccgg
     7261 agagcgatcc cggcctgttc ctcgaatact ggcaactgcc ggaagagacg gccaaattgc
     7321 gtcatgacgg ctggttcttc acgggcgatt atgcgcgtta cgacgaggac ggctatatct
     7381 ggttcctcgg ccgcaaggat gacatcatca agagtttcgg ctaccgcgtc tcgccctacg
     7441 agatcgagcg tgtat
//
LOCUS       DATMTW010000028         7450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_338136, whole genome shotgun sequence.
ACCESSION   DATMTW010000028 DATMTW010000000
VERSION     DATMTW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338136"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VN084_00555"
     CDS             complement(<1..401)
                     /locus_tag="VN084_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27971.1"
                     /translation="MENNVSPEFLRQRRAVHHGLCSVLSEDEAITVLNQWVSHIGSSG
                     SVFNGLNVFVRNVCTQLGKSELQREMLQSINRALMAREEELRAAPDLSGTKLVAEKAV
                     SSSSGKSAPAETAAPASESAEFLGFQALLRAL"
     gene            complement(763..3477)
                     /gene="secA"
                     /locus_tag="VN084_00560"
     CDS             complement(763..3477)
                     /gene="secA"
                     /locus_tag="VN084_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWT27972.1"
                     /translation="MLSTLFKKVFGSRNDRLVKQYSNNVRLINELEPAMQALTDAQLR
                     AKTEEFKQRYQNGESLDKLLPEAFAVVREGGRRILEMRHFDVQLIGGMVLHAGKIAEM
                     RTGEGKTLVATLPAYLNALSGKGVHVVTVNDYLAKRDAEWMARLYNFLGLTVGINLSQ
                     MPHDEKQKAYAADITYGTNNEFGFDYLRDNMVYTAEERVQRALNYALVDEVDSILIDE
                     ARTPLIISGQADDSIDLYTQMNGIAAKLVRQETEEGEGDYWVDEKSQQVLLSEAGHEH
                     AENLLAEAGLLPEGGSLYDAANITLVHHMYAALRARSLFHRDQHYVVRDGQVVIVDEF
                     TGRMMTGRRWSDGLHQAVEAKEGMAIQKENQTLASITFQNYFRMYGKLSGMTGTADTE
                     AYEFNQIYGLETVVIPTHRPMQRKDQMDKVYRTAKEKYDAVIEDIVECQKRGQPVLVG
                     TTSIENSELISELLNKAKLDHQVLNAKQHEREAQIIVQAGRPGVITIATNMAGRGTDI
                     VLGGNPEPDVAAVRSDETLSDADKESRIAAIKADWQKVHEAVLAAGGLHIIGTERHES
                     RRVDNQLRGRSGRQGDPGSSRFYLSLEDQLLRIFASDRVSAIMERLKMPEGEAIEHPW
                     VTRAIENAQRKVEGRNFDIRKQLLEYDDVANDQRKVIYQQRNELLEATDVGETIAAMR
                     QDVIIDIIATHIPPGSVEEQWDIEGLEKALQADVGLDMPLQQMLTDNPDLHEETLRER
                     ILAQAETNYAAKEAMASADVMRQFERAIMLQSLDNHWREHLAALDHLRQGIHLRSYAQ
                     KNPKQEYKREAFELFAALLDTVKREVTQVTMLVQVRSEADVEAVEKPAEPENVRYQHA
                     DYDDALGNAPEPQDAIRPFEREGIKVGRNEPCPCGSGKKYKQCHGKLS"
     gene            complement(3568..4449)
                     /locus_tag="VN084_00565"
     CDS             complement(3568..4449)
                     /locus_tag="VN084_00565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWT27973.1"
                     /translation="MNIIFVSNNMAKARTLTLVQAALLLVAVVAIAVVLTLAIILPQG
                     DGKHQGVKHLLPNPLNFTLHNPQEHLDALALQLGELQARVMRLDALSDRLSRLAGVKD
                     KDVAQQPVPGRGGPEINAQGMTEQQLQQKINELTQEIEQRSDHLSMLEALFLQQRMKK
                     NTLPSSSPVNASYNSSSYGWRIDPFTGRSAFHEGLDFVAEVGTPIYAAADGIVTTAEQ
                     TPDYGKIVKIDHGSGLETRYAHASLLLVKVGDRVSKGQVIAQVGTTGRSTGPHLHFEV
                     RLNGAALDPRKYLRKVN"
     gene            4477..4893
                     /locus_tag="VN084_00570"
     CDS             4477..4893
                     /locus_tag="VN084_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HWT27974.1"
                     /translation="MRRINALFRNDAALMALHDHADSLSAMQATWSAVCPDSLKPLTR
                     VGALNKGTLTVYTDLGAVAAKIKLLLPSLLTKLQKRGIEVTAIRVRVQVKSEPQPKAK
                     IQRKISAKGASRLASLAKELEGSALGEVLERLSRRT"
     gene            4923..5714
                     /locus_tag="VN084_00575"
     CDS             4923..5714
                     /locus_tag="VN084_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWT27975.1"
                     /translation="MESLLYLLAGALAGLLSGLFGVGGGLIIVPILVALFSLQHFADA
                     HIMHLALGSSLATIVVTSLASARAHHRRDNVNWAVVGRITPGIVLGTLLGSQLATCLD
                     SDGLKAIFAIFVLAVATQMLLNLRPNPHRQLPGSLATSFVGGVIGGVSSLVGIGGGTL
                     SVPFLLYCNTAIHRAIGTSAAIGLPIALAGATGYVLAGWGQTNLPQYSLGFLYLPAVA
                     GIAIASSLTAPFGVMLAQRFSAKGLKRLFAILLYGIGVKMAWGLF"
     gene            5711..6463
                     /locus_tag="VN084_00580"
     CDS             5711..6463
                     /locus_tag="VN084_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWT27976.1"
                     /translation="MSASQERYVIEAQGLSKVFDQLSAVDDISFEVRRGVTTALLGGN
                     GAGKTTTIAMLLGLLLPSAGSIRILGEDMLQHRHRLLHRMNFSSPYVDLPQRLTVREN
                     LTIYGHLYRLPRLKQRVAQIASELHLDEVLDRRYGRLSAGQKTRVSLAKSLLNEPELL
                     LLDEPTASLDPDTADAIRTHLEQYQLRTGASILLASHNMAEVERMAADVIMMRRGKIV
                     DTGAPATLIDRYGRNSMEEVFIDVARNREVTA"
     gene            6460..7272
                     /locus_tag="VN084_00585"
     CDS             6460..7272
                     /locus_tag="VN084_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWT27977.1"
                     /translation="MTQTDVRFSPRRIGALVLRHLYLMRTSWPRVLEMVYWPTVQMVL
                     WGFITLFLQGNSSYVAQAAGILLSAVLLWDVLFRGQLGIALVFMEEMWSRNLGHLFVS
                     PLRPYEMVASLILMSLIRTLIGVGGAALVAIPLFHYNIFDLGLPLIAFFFNLVIMGWA
                     VGLLVSGIVLRYGLGAESVAWIAVFAIQPLCAIYYPVDVLPGWLQPVAWALPASHVFE
                     GMRELLFRHSFNWHHFSYAAGLNVLFLSAGIAAFLGYFRAARQRGLLLHVGE"
     gene            complement(7272..7406)
                     /locus_tag="VN084_00590"
                     /pseudo
     CDS             complement(7272..7406)
                     /locus_tag="VN084_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1361 a         2247 c         2168 g         1674 t
ORIGIN      
        1 agcgcccgca gcaatgcctg gaaaccgagg aactcggcgc tctcgctggc gggcgccgct
       61 gtctctgcag gcgcggactt gccactgctg ctggatacgg ctttttcagc taccagcttg
      121 gtgccggaga gatcaggtgc cgcgcgcaac tcctcttcgc gcgccatcag cgcacggttg
      181 atcgattgca gcatctcgcg ctgcagctca ctcttgccga gctgcgtaca gacgttgcgg
      241 acgaacacat tgaggccatt gaagacggaa ccggaactac cgatgtggct gacccactga
      301 ttgagtacgg tgatcgcttc gtcttcgctc agcaccgagc aaagaccgtg atgcactgcg
      361 cgacgttgac gcaggaattc gggactgacg ttgttttcca ttattagctg attcaccttg
      421 ttgttaatcg aacggcattg tcttttcaca acatgcccca ttttgtggcg gggccttgta
      481 gatggtgcgc gattgtcgtg cgtctgcccg atgttcggat caggccgtgc atgctgcgcc
      541 ttgtccatcc tcgcagccag tattgtcgcg gcatccggtg gcatgccgat acccgccgtt
      601 aggtatccac cactgtctac gaaggaggag tcgcaagact tgtgccaagc cccattcctg
      661 cccgcgatcg aggccggcga ccggcaataa aaaaccccgt agcgttcgca acggggttct
      721 agtcagtgac cggcaatatc gggccggtat ggattcaggg catcagctca gtttgccatg
      781 acattgcttg tactttttgc cggaaccgca aggacatggc tcattgcgac cgaccttgat
      841 gccttcgcgc tcgaacgggc ggatcgcgtc ttgcggctcg ggggcattgc caagcgcatc
      901 gtcgtagtcg gcgtgctgat agcgcacgtt ttctggctcc gccggctttt ccacggcttc
      961 gacgtcggct tcgctgcgca cctgcaccag catggtcacc tgcgtgacct cgcgcttcac
     1021 ggtatcgagc agcgctgcga aaagttcgaa cgcctcgcgt ttatattcct gtttgggatt
     1081 cttctgcgcg taggaacgca gatggatgcc ttggcgcaga tggtctagcg ccgccaagtg
     1141 ctcgcgccag tgattgtcga gactctgcaa catgatcgcg cgctcgaatt ggcgcatgac
     1201 atcggcagac gccatcgcct ccttggccgc atagttggtt tccgcctgcg ccaagatacg
     1261 ctcgcgcagg gtttcctcgt gcaggtctgg attgtccgtc aacatctgct gaagcggcat
     1321 atcgaggccg acatcggctt gcagggcttt ttcaaggcct tcgatgtccc actgttcctc
     1381 gacactgccc ggtggaatat gggtagcgat gatatcgatg atgacgtcct ggcgcatggc
     1441 tgcgatggtc tcgccgacat cggtcgcttc cagcagttcg ttacgttgct ggtagatcac
     1501 cttgcgttgg tcgttggcga catcgtcata ttccagcagt tgcttgcgga tatcgaagtt
     1561 gcggccttcg accttacgct gggcattctc gatcgcgcgc gtcacccaag gatgctcgat
     1621 cgcttcgcct tcaggcatct tcagccgctc catgatcgcc gatacgcggt cggaggcgaa
     1681 gatgcgaagc aactggtctt ccagcgagag gtagaagcgg ctcgaacccg ggtcgccctg
     1741 acggccggag cggccgcgca gctggttatc gacgcggcgg gattcgtgac gctcggtacc
     1801 gatgatgtgc aagccgccgg ctgccagcac ggcttcgtga accttctgcc aatcggcctt
     1861 gatggcggcg atacggcttt ccttgtcggc gtcgctcaat gtctcgtcgc tgcgcacagc
     1921 ggcgacatcc ggttccggat tgccgccgag gacgatgtcg gtgccgcggc cggccatgtt
     1981 ggtagcgatg gtgatgacgc ctggacgccc ggcctgcacg atgatctgtg cctcacgttc
     2041 gtgctgtttg gcgttcagta cctggtgatc gagcttggcc ttgttcagta gctcggagat
     2101 cagttcggag ttctcgatgg aggtcgtgcc gaccagcact ggctggccac gtttctggca
     2161 ttcgacgatg tcctcgatca ccgcatcgta tttctccttg gcggtgcgat agaccttatc
     2221 catctggtcc ttgcgctgca tgggccgatg ggtagggatg acgaccgtct caagaccata
     2281 gatctgattg aattcgtaag cttcggtatc ggcggtgccg gtcatgccgg acagctttcc
     2341 atacatacgg aagtaattct ggaaggtgat cgacgccagc gtctggttct ccttctggat
     2401 cgccatgcct tccttcgctt cgaccgcctg atgcaggccg tctgaccatc gacggccagt
     2461 catcatccgg ccagtgaact catcgacgat cacgacctgg ccgtcacgca ccacgtagtg
     2521 ctggtcacgg tggaacaggg agcgcgcgcg cagggccgca tacatgtgat gcaccagcgt
     2581 gatgttggcg gcatcgtaga ggcttccgcc ctctggcaac aggccggcct cggccagcag
     2641 gttctccgca tgttcgtggc ccgcttccga gagcaggact tgctgcgatt tctcgtcgac
     2701 ccagtaatcg ccctcgcctt cctcggtttc ctggcgaacc agcttggcgg cgataccgtt
     2761 catctgtgtg tagagatcga tgctgtcgtc cgcctgcccg gagatgatca gcggggttcg
     2821 ggcttcgtcg atcaggatgg agtcgacctc gtccaccagc gcataattga gcgcacgctg
     2881 cacgcgctcc tctgcggtgt agaccatgtt gtcgcgcagg taatcaaaac cgaattcgtt
     2941 attggtgccg tacgtgatgt cggccgcata ggccttctgc ttttcgtcgt gcggcatctg
     3001 cgacaggttg atgccaacgg tcagcccaag gaagttatag agccgcgcca tccattctgc
     3061 gtcacgtttg gcgagatagt cattgaccgt cactacgtgc acgcctttac cggagagggc
     3121 attgaggtag gccgggagcg tcgcgaccag cgtcttgcct tcgccggtgc gcatttcggc
     3181 gatcttgccg gcatgcaaca ccatgccgcc gatcaactgc acatcgaaat gccgcatctc
     3241 cagtatccga cggccacctt cacgcaccac ggcaaaggct tccggcagca gcttgtcgag
     3301 cgactcgccg ttctggtaac gctgtttgaa ttcttcggtc ttggcacgca gctgcgcatc
     3361 ggtgagcgcc tgcatcgcgg gttcgagctc gttgatgaga cggacattat tggagtactg
     3421 cttgaccagc cgatcgttac ggctaccgaa tactttcttg aacaacgtgg ataacatgaa
     3481 ggaacaccaa ctttccgcga cgcggaagcc ttaaggttga ctttggaaga ttagaaatac
     3541 aacgaattgg tacctgtata ttcctgctta gttcacttta cgcaaatatt ttctcggatc
     3601 cagcgcggcc ccattgaggc ggacctcgaa atgcagatgt ggccctgtcg aacggccagt
     3661 ggtgccgacc tgggcgatga cctgcccctt gcttacgcga tcgccgactt tcaccagcag
     3721 caaggaagcg tgcgcataac gcgtctccaa gccggatccg tgatcgatct tgacgatttt
     3781 accatagtcg ggggtctgtt ccgccgtcgt cacgatgccg tctgccgccg cgtagatcgg
     3841 ggtgccgact tccgccacga aatccagtcc ttcatgaaaa gccgaccggc cggtgaatgg
     3901 atcgatacgc cagccatagc tggaagagtt gtaacttgca ttgaccgggc tactgctcgg
     3961 caaggtgttc tttttcatgc gctgctgcag gaacagggcc tccagcatgc tcaagtgatc
     4021 gctgcgctgc tcgatctcct gcgtgagttc gttgatcttt tgttgcaact gctgctcggt
     4081 catgccttgc gcgttgatct ccggaccgcc acgccctggt acgggttgct gtgcgacatc
     4141 cttatccttg acaccggcca ggcgtgaaag ccggtcgctc agggcatcaa gacgcatgac
     4201 acgcgcctgc agttcgccaa gctgcaaggc gagcgcatcg aggtgttcct ggggattgtg
     4261 caaagtgaaa ttgagcgggt tggggagcag gtgcttgacg ccttggtgct tgccgtcgcc
     4321 ctgtggcaga atgattgcga gtgtcaggac aacagcgatc gccacgacgg cgaccagtag
     4381 caaagccgcc tggactagcg tcaaggtcct ggcttttgcc atgttgttcg agacaaagat
     4441 aatgttcatt cattcccctt tgggaggctc cgcggcatgc gtcgcatcaa tgctttgttc
     4501 cgaaacgatg ccgctctcat ggcactgcac gaccatgcgg acagcctgtc tgccatgcaa
     4561 gcgacatggt ctgcggtctg tccggactcc ctgaagccac tgacacgcgt cggcgccctc
     4621 aacaagggca ccttgacggt gtataccgat ctaggcgccg tggcggccaa gatcaaatta
     4681 ttattgccaa gcttgttgac caagctgcaa aagcgaggaa tcgaagttac tgcaattcgc
     4741 gttcgagtgc aagtcaaatc tgagccgcag ccaaaggcca aaatacaacg taaaatcagt
     4801 gccaaaggcg cttccaggct ggcttcatta gcgaaggaac tggaaggttc ggcgctcggc
     4861 gaagtactgg aaagactgtc gcgcaggacc tgaccagcgc ctagcctcat ccggacattc
     4921 gcatggaatc tctgctttat cttctcgctg gcgcgctcgc cggattgcta tcggggttgt
     4981 tcggcgtcgg cggcggtctc atcatcgttc ccatcctggt ggcgctgttc agcctgcaac
     5041 attttgccga cgcccacatc atgcacttgg ccctgggctc ctcgctggca accatcgtcg
     5101 tcacgtccct cgcctcggcc cgtgcacatc accgtcgtga caacgtaaac tgggctgtcg
     5161 tcggtcgcat cacgcccggc atcgttctcg gcacgctact cggcagccag ctcgccacct
     5221 gcctggacag cgatgggctt aaggccatct ttgccatctt cgtgctggcg gtcgccacac
     5281 agatgctcct gaacttgcgc ccgaatccac accgccagct accgggtagc ctagccacca
     5341 gcttcgtcgg cggcgtcatc ggcggcgtct ccagtctggt cggtatcggc gggggaacgc
     5401 tgtcggtacc ctttctgctt tattgcaaca cggcaatcca tcgcgccatc ggcacctctg
     5461 ccgccatcgg gctgccgatc gccctggctg gcgccactgg ttacgtgctg gctggctggg
     5521 gccagacaaa tctgccgcaa tatagtctgg ggttcctcta cttgccagcc gttgccggga
     5581 tcgccatcgc cagcagcctg accgcgcctt ttggcgtgat gctggcgcaa cgtttttccg
     5641 cgaaaggact caaacgcctg tttgccatcc ttttatatgg catcggtgta aaaatggctt
     5701 gggggttgtt ttgagtgcat cgcaagaaag atatgtcatc gaggcgcaag ggctcagcaa
     5761 agtattcgac cagttgagcg cggtcgacga tatcagtttt gaagtccgac gcggcgtcac
     5821 gacggcattg ctgggcggca acggcgccgg caagaccacc acgatcgcca tgctgctcgg
     5881 cctgctgctg cccagcgcgg gcagcatccg catcctcggt gaagacatgt tgcagcatcg
     5941 ccaccgctta ctgcatcgca tgaatttctc gtcgccgtac gtcgacttgc cgcagcgact
     6001 cacagtccgt gagaacctga ccatctacgg ccatctctat cgcttgccaa gactcaagca
     6061 gcgggtcgcg cagatcgcga gtgaactcca tctggacgaa gtactcgacc gtcgctatgg
     6121 gcggctctca gctggacaga agacccgcgt ctcgcttgcc aaatcgctgc tcaatgagcc
     6181 ggaactgctt ttgctcgacg aacctaccgc atcgctcgac ccagacacag cggacgccat
     6241 ccgcacccac cttgagcaat atcagctgcg caccggcgcc agcatcctgc tggcttcgca
     6301 caatatggct gaggtggaac gcatggccgc ggatgtcatc atgatgcgac gcggcaagat
     6361 cgtcgatacc ggggcgcccg caaccctgat cgaccgctat ggccgcaata gcatggaaga
     6421 ggtcttcatc gatgtcgccc gcaaccgcga ggtgaccgca tgacgcaaac cgatgtacga
     6481 ttctcgcccc gccgcatcgg cgcgctggtg ctgcgtcatc tctacctcat gcgcacgtcc
     6541 tggccacgtg tgctggagat ggtctactgg ccgacggtac agatggtgct gtggggattc
     6601 atcacgctgt tcctgcaggg caacagcagc tatgtggcgc aggctgccgg catcctgctc
     6661 tcggccgtac tgctctggga tgtgctgttt cgcggccaac tcggcatcgc gctcgtgttc
     6721 atggaagaga tgtggtcgcg caacctgggc catctgttcg tcagcccctt gcgaccctac
     6781 gagatggtcg cttcattgat actgatgagc ctgatccgca ccctgatcgg tgtgggtggg
     6841 gcggccctgg tcgcgatccc tctgttccat tacaacatct tcgaccttgg cctgccgctg
     6901 atcgccttct tcttcaacct tgtcatcatg ggctgggccg tgggcctgct agtgtcaggc
     6961 atcgtattgc gttacgggct aggtgccgag agcgtcgcct ggatcgcggt cttcgccatc
     7021 cagccgttat gcgcgatcta ctatccggtc gacgtgctac cgggctggct gcagccggtc
     7081 gcttgggcgc tgccagccag ccacgtgttc gagggcatgc gggagttgtt gttccgtcac
     7141 agcttcaact ggcaccactt cagttatgcg gcaggcctca atgtactctt cctcagtgcg
     7201 ggcatcgccg cgttcctcgg atacttccgc gcggcgcgcc agcgcggact gctgctgcac
     7261 gtgggcgaat aagcccggct caccgctgcc ggcacaagca accaaataga accgttctgc
     7321 ttcaaccaga gataaggcag gtaacaaccg acgatctcgg ccatggcggt gagtgcgaat
     7381 aacccgaaag tcttgaatgc cagcatagcg aggcccgatc aaatgaaagt gcttcgattc
     7441 tagcgttaca
//
LOCUS       DATMTW010000029         1634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_506029, whole genome shotgun sequence.
ACCESSION   DATMTW010000029 DATMTW010000000
VERSION     DATMTW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506029"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="bioF"
                     /locus_tag="VN084_00595"
     CDS             complement(<1..1088)
                     /gene="bioF"
                     /locus_tag="VN084_00595"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWT27978.1"
                     /translation="MSQRLYSELQAELDARASAGLTRRRRLLQGPQGTAITCDGEAVL
                     SFCSNDYLGLASHPALADAIKAAVDSVGVGAGASHLITGHHELHETLEQALATFVGLP
                     AALLFSTGYMANLGVVSALMGRDDAIFADKLNHASLNDAAVLARATLHRYAHNDLAAL
                     ENLLAESKAKRKLVVADAVFSMDGDVAPVPQLLALCEYHDAYLLLDDAHGFGVLGEQG
                     AGILDHFAVSSPRIIYMATLGKAAGVAGAFVAGETVLIDYLVQQCRPYIYTTASPAIL
                     SAALLAALQVMREDPARHAHLQQLIRLLKTRLKLDRWQLMPSDTAIQPLLVGSNEKAV
                     RISEWLLTQGMLVPAIRPPTVPKGTARLR"
     gene            complement(1088..>1634)
                     /gene="bioB"
                     /locus_tag="VN084_00600"
     CDS             complement(1088..>1634)
                     /gene="bioB"
                     /locus_tag="VN084_00600"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWT27979.1"
                     /translation="MLKEGQAEQLKDAGLDYYNHNLDTAPEFYGDVISTRTYQDRLET
                     LERVRNVDINVCCGGIIGMGESRNQRAGLLAQLANMERPPESVPINLLTQVEGTPLHG
                     TDELDPMEFVRTIAAARITMPTSYVRLSAGRQSMHEGIQALCFLAGANSIFYGEKLLT
                     TGNPEAEADKALFAKLGIKAI"
BASE COUNT          306 a          501 c          509 g          318 t
ORIGIN      
        1 cgcaagcgtg cggtgccctt gggtacggtc ggcggacgga tcgccggcac cagcatgcct
       61 tgtgtcagca accactcact tatccggaca gctttttcgt tgctgcccac cagcaatggc
      121 tggatcgccg tatcggaagg catcagttgc cagcggtcga gcttgagtcg tgtcttcaag
      181 agacggatca gctgctgcag atgggcgtga cgcgcaggat cttctcgcat cacctgcagc
      241 gccgccagca gggccgcgga gaggatcgcg gggctggcgg tggtatagat gtagggccgg
      301 cattgctgca cgagataatc gatcagcacc gtttcgccgg cgacgaacgc tcctgccact
      361 ccggccgcct tgcccagtgt cgccatgtag atgatacgcg gcgaactgac cgcgaaatga
      421 tcgaggatcc ctgcgccctg ttcgcccagc acgccaaaac cgtgcgcatc gtccagcaac
      481 agataggcgt catgatactc gcacaatgcc agtagttgcg gtaccggggc cacatcgccg
      541 tccatgctga acaccgcgtc ggcgaccacg agtttgcgtt tggccttgga ctccgccagc
      601 aggttttcca gggcagcgag atcgttatgt gcatagcgat gcagggttgc gcgcgcgagc
      661 actgcggcat cgttgagcga ggcatggttg agcttgtcgg caaagatcgc gtcatccctg
      721 cccatcaggg cactgaccac cccgagattg gccatatagc cagtggagaa tagcagggcc
      781 gcaggcaagc cgacgaatgt cgccagcgcc tgctccagcg tctcgtgcaa ctcatgatgg
      841 ccggtgatga gatgcgaggc accggcaccg acccccacgc tatcgactgc ggccttgatg
      901 gcatcggcca gcgctggatg gctggcgagc cctagatagt cgttactgca aaaggacagc
      961 accgcttcgc cgtcgcaggt gatggcggtt ccctgaggcc cttgcagcag acgccggcgc
     1021 cgggtcaatc cggcgctcgc gcgtgcatcc agctcagcct gcagctcgga atacaagcgc
     1081 tgcgacatta aatcgctttg atgcccagct tggcaaagag cgccttgtct gcctcggctt
     1141 ccgggttacc cgtggtcaac agcttttcgc cgtagaagat ggagttggcg ccggcaagga
     1201 aacacagtgc ctggatgccc tcatgcatgc tctgacggcc cgcggacaag cgcacatagc
     1261 tggttggcat ggtgatgcgc gcagcggcga tggtacgcac gaattccatt gggtcgagct
     1321 catccgtgcc atgcagcggc gtgccctcga cttgcgtcag gagattgatg ggaacgctct
     1381 ccggcgggcg ctccatgttg gccagttgtg ccagcagccc cgcacgctgg ttgcgcgatt
     1441 cacccatgcc gatgatgccg ccacagcaga cgttgatatc gacgttacgc acgcgctcca
     1501 gcgtctccag acggtcctgg taagtacgcg tgctgatcac atcgccgtag aactctggcg
     1561 cagtgtcgag gttatggttg tagtaatcga ggccagcgtc cttcagttgc tcggcctgac
     1621 cttccttcag catg
//
LOCUS       DATMTW010000030         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_237823, whole genome shotgun sequence.
ACCESSION   DATMTW010000030 DATMTW010000000
VERSION     DATMTW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237823"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1007
                     /locus_tag="VN084_00605"
     CDS             6..1007
                     /locus_tag="VN084_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWT27980.1"
                     /translation="MADWREHALKLESEINKVVVGQQSPIRLITTSIFARGHVLLEGD
                     VGVGKTTLLRAFTRGIGGGYQRIEGTIDLMPNDLVYHTYLGEDGKPHVSPGPLLMSGE
                     NLSVFFFNEINRARPLVHSLLLRVMAERTLTAFNKEHHFPHMLVFADRNQVEKEETFE
                     IPSAARDRFMMEIPIVVPSDNDIMESLVFDTRFHNVDALVDTVPADILKFDELNAFAG
                     VIQSSVEASPTLRKYALELWLATKTPTHYGIKVAGVDMNRLVLAGASPRGMSMMLRAA
                     RVNAWLHDRSAVLPEDIHAVFHATVAHRLVFSPVYEMRRTEIAREMLTGITNAIAAP"
     gene            1154..2017
                     /locus_tag="VN084_00610"
     CDS             1154..2017
                     /locus_tag="VN084_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWT27981.1"
                     /translation="MYNFDIKEFSYHIGWRSRGRHPGQHSSTQRGMGMEFRGHTTLLS
                     YPDPRRIDIRQTIRDPLEQVYVRLFNQKSSTPVYVICDLSGSMNFGSRHRKIKLAAEI
                     AESVAQSSTRAADPFGFIGFDETVREDWVCTASVKPQRAFELTERLKEYHPHAASAEG
                     LRNAWRFLPRERSLVFLISDFHMPFELLEDSLAMLLRHQIVPMVLWDSAEYRNLPEFG
                     ITSVADSETGARRTLLMRKELRDNIVASFEARREAIYNLFMRFDMPPFFVEGDFDADA
                     MTEYFYQFVAA"
     gene            2060..>2502
                     /locus_tag="VN084_00615"
     CDS             2060..>2502
                     /locus_tag="VN084_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27982.1"
                     /translation="MRSNLLALFMLALLPASQAIAEELPPNIEDGIVSIKIVEPTHNV
                     GYTVGDILERDITLEVKKPYKLIDSSLPIVGYEKRYKGQTLGVDLRSISHTQEDGKDS
                     TVHHIKLAYQVFTNNVVAKHASLPAEFIRLIKDGKVAQYRIPSWG"
BASE COUNT          506 a          805 c          697 g          494 t
ORIGIN      
        1 gtctattggc cgactggcgc gagcatgcgc taaaactgga atcggaaatc aacaaggtag
       61 tggtgggcca gcaatcgcct attcgcctga tcaccacctc catcttcgcg cgcggccacg
      121 tgttgctcga aggcgatgtc ggcgtgggca agaccacgct gctgcgtgcc ttcacacgcg
      181 gcatcggtgg cggctaccag cgtatcgaag gcaccatcga cttgatgccg aacgatctgg
      241 tgtaccacac ctacctcggc gaagacggca agccacacgt cagcccaggc ccgctgctga
      301 tgtcgggcga gaacctgtcc gtgttcttct tcaacgagat caaccgcgcc cgcccgctgg
      361 tgcattcgct gctgctgcgc gtgatggcgg aacgcacgct gacagcattc aacaaggaac
      421 atcacttccc gcacatgctg gtgttcgctg accgtaacca ggtggagaag gaagagacct
      481 tcgagatccc atccgcggcg cgtgaccgct tcatgatgga gatccccatc gtcgtgccga
      541 gcgacaacga catcatggaa tcgctggtgt tcgacacccg cttccacaac gtcgatgcgc
      601 tggtcgatac cgtgcctgcc gacatcctca agttcgacga gctgaacgcc tttgccggcg
      661 tgatccagtc cagcgtcgaa gccagcccca ccctgcgcaa atatgcgctc gaactgtggc
      721 tcgccaccaa gacgccgact cattacggca tcaaggtcgc tggcgtggac atgaaccgcc
      781 tggtactggc cggcgccagc ccacgcggca tgagcatgat gttgcgcgca gcccgtgtga
      841 acgcctggct gcatgaccgc agcgccgtgt tgccggaaga catccacgcc gtgttccacg
      901 ccaccgtggc ccaccgcctg gtattcagcc cggtctacga gatgcgccgc acggaaatcg
      961 cgcgggagat gttgacgggg attactaacg ctatcgccgc cccgtgatcg atctgtctca
     1021 catcgacgtt actttgttgc cagcgtcgct tcggccttgc cgtacttcca tgtactgcct
     1081 tcatccgaag ctccttggcg ccgcgtatcg tcttcctgat acaaatcgct cgattgagat
     1141 ggcctgacag attatgtaca acttcgatat caaggaattc agctaccaca tcggctggcg
     1201 ctcgcgtggg cgccacccgg gtcagcattc cagcacgcaa cgcggcatgg gcatggagtt
     1261 ccgtgggcat acgaccctgc tctcctaccc tgacccgcgt cgcatcgaca tccgccagac
     1321 catccgcgac ccgctggagc aggtgtatgt gcgcctgttc aaccagaaga gttcgacgcc
     1381 ggtgtatgtc atctgcgatc tgtcgggctc gatgaatttc ggcagccgcc accgcaagat
     1441 caagctcgct gccgagatcg ccgaatctgt ggcgcagtcc tcgacacgcg cggccgatcc
     1501 gttcggtttc atcggtttcg acgagaccgt tcgcgaagac tgggtatgca ccgcttcggt
     1561 gaaaccgcag cgcgcgttcg aactgaccga gcggctcaag gaataccatc cgcatgcggc
     1621 ctcggccgag ggcttgcgca atgcctggcg tttcctgccg cgcgagcgtt cgctggtgtt
     1681 cctgatctcg gatttccata tgccgttcga gctgctggaa gattcgctgg ccatgctctt
     1741 gcgtcaccag atcgtgccca tggtgctgtg ggattcagcg gaataccgca acctccccga
     1801 gttcggcatc accagcgtcg ccgacagcga gaccggcgcc cggcgtaccc tgctcatgcg
     1861 caaggaactg cgcgacaaca tcgtggcctc gttcgaagcc aggcgtgaag cgatctacaa
     1921 cctcttcatg cgattcgaca tgccgccgtt cttcgtcgaa ggcgatttcg acgccgatgc
     1981 catgaccgaa tacttttatc aatttgtcgc agcctagccc gaagcgcccc atccaatgaa
     2041 gaaccctata gtcatgcaca tgcgatccaa tctcctagcc ttgttcatgc tggccctgct
     2101 gcccgccagc caagccatcg ccgaagaatt gccgccgaat atcgaggacg gcatcgtcag
     2161 tatcaagatc gtcgagccga cccacaatgt cggctacacg gtaggcgata tcctcgagcg
     2221 tgatatcacc ttggaagtga agaagcctta caagctgatc gacagttcgt tgccgatcgt
     2281 tggctacgag aaacgctaca aaggccagac cctgggcgtg gatctcagga gcatcagtca
     2341 cacccaggag gacggcaagg acagcacggt gcatcacatc aagctggcct accaggtctt
     2401 caccaacaat gtggtggcca agcatgccag cctgcctgcc gagttcatcc gcctgattaa
     2461 ggacggcaag gtcgcccagt accgcatccc aagctggggc tt
//
LOCUS       DATMTW010000031         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_70461, whole genome shotgun sequence.
ACCESSION   DATMTW010000031 DATMTW010000000
VERSION     DATMTW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70461"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="dapC"
                     /locus_tag="VN084_00620"
     CDS             <1..1054
                     /gene="dapC"
                     /locus_tag="VN084_00620"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HWT27983.1"
                     /translation="TPALIRDALVQNLGGLANYPTTAGIAELRQAISAWLSRRYQIPA
                     LDPEKAIIPVNGSREALFAFAQAVIDGSRPAPVVISPNPFYQIYEGAAFLAGAEPYFL
                     NTLPENDFAMDFDSVPESVLQRTQLAYLCSPGNPSGKVMTLAQWQQVFALSDYYGFVI
                     AADECYSEIYFDEANPPLGALQAAHQLGRDFTRLVIFSSLSKRSNVPGMRSGFVAGDA
                     QILERFLLYRTYHGCAMSPAVQAASIAAWNDEAHVVENRRLYDEKFKEVTPMLQSAME
                     TAIPDGAFYLWARVSGSDTEFARRLYQERNVTVLPGSYLAREAHGVNPGLNFVRLALV
                     ASLEECREAAQRILSL"
     gene            1077..1625
                     /gene="fae"
                     /locus_tag="VN084_00625"
     CDS             1077..1625
                     /gene="fae"
                     /locus_tag="VN084_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HWT27984.1"
                     /translation="MNERIVMRVGEALVAGGPAGTAAEPEVVIGEMDGPMGTAFANLL
                     GDQVKGHTRVLAIMNTDIMVRPATIMVSKVTVKDTRYTNILMGTVQAAIANGVLDAVR
                     CGDIPKEKANDLGIIVSVWLNPIVAEQDDLDHQILFDIHRKATAAAIKKAMRNEPSID
                     WLLENQEKIVHKYFQMGLDGKI"
     gene            complement(1651..>2498)
                     /gene="miaA"
                     /locus_tag="VN084_00630"
     CDS             complement(1651..>2498)
                     /gene="miaA"
                     /locus_tag="VN084_00630"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWT27985.1"
                     /translation="VDSALVYRDMDIGTAKPDAATLKLAPHHLIDIIDPTDAYSAAQF
                     RTAALKLMAEITARGKVPVLVGGTMLYFKALQGGLNDLPEADPETRAALDAEALKVGW
                     PGMHAKLAAIDPETAARLKTTDAQRIQRALEVYALSGKPMSQIFAEEKTQSALPYRLL
                     KLALVPSDRKALHARIALRFEQMLEAGLLDEVRALRVKYPTLHLGLPAMRCVGYRQAW
                     QYLDGEFDMDALREKGIAATRQLAKRQLTWLRGMDDIVELDCLDTGLNETIVTYINQF
                     IRDSS"
BASE COUNT          514 a          790 c          716 g          478 t
ORIGIN      
        1 aacaccagcc ttgatccggg atgcactggt gcaaaacctc ggcgggttgg ccaattatcc
       61 cacgactgcc ggtatcgccg agctacgcca ggcgatttct gcatggctga gccgccgtta
      121 ccagataccg gcactcgatc cggaaaaagc catcatcccc gtcaacggta gccgcgaggc
      181 cctgttcgcc ttcgctcagg cagtcatcga cggcagccgc ccggcacctg tcgtgatctc
      241 gcccaacccg ttctaccaga tctacgaggg tgcggccttc ctcgcgggcg cagaacccta
      301 tttcctcaat acgctgcccg agaacgactt cgccatggat ttcgacagcg tgccggagag
      361 cgtgttacag cgcacgcaac tggcctacct gtgctcgccg ggcaatccgt cgggcaaggt
      421 catgacgctg gcacaatggc agcaggtgtt cgcgctgtca gactactacg gcttcgtcat
      481 cgccgccgac gaatgctact cggagatcta cttcgacgag gccaacccgc cgctgggcgc
      541 gctgcaagcc gctcaccagc tgggtcgcga tttcactcgc ctggtgatct tcagctccct
      601 gtccaagcgt tccaacgtgc cgggcatgcg ctccggcttc gttgccggcg atgcccagat
      661 actggaaaga ttcctgctct accgcaccta tcacggctgc gccatgagtc cggcggtgca
      721 ggctgccagc atcgctgcct ggaacgacga agcgcacgtc gtcgagaatc gccggctgta
      781 cgacgaaaaa ttcaaggaag tcacccccat gctgcagtca gcgatggaaa cggcgatccc
      841 ggacggcgcc ttctatctct gggcgcgtgt atcaggctca gataccgaat tcgcgcggcg
      901 cctctaccag gagcggaatg tcaccgtatt gcccggcagc tacctcgcgc gcgaggcgca
      961 cggcgtcaat cccgggttga acttcgtgcg tctggcgctg gtcgcttcat tagaggaatg
     1021 cagggaagca gcccagcgta tcctcagtct ttagttttca atcaggagta aacaatatga
     1081 atgaacgcat cgtgatgcgc gtaggcgaag ctttggtcgc aggcggcccc gcaggtacag
     1141 cggcagaacc cgaagtcgtg atcggcgaga tggatggccc gatgggcaca gccttcgcca
     1201 acctgcttgg cgaccaggtc aagggccaca cccgcgtgct ggccatcatg aacaccgaca
     1261 tcatggtgcg ccccgccacc atcatggtca gcaaggtcac ggtgaaggac acccgctaca
     1321 ccaacatcct gatgggcacg gtgcaggctg ccatcgccaa tggcgtgctc gacgctgtgc
     1381 gctgcggcga tatccccaag gaaaaggcca atgatctcgg catcatcgtc tcggtctggc
     1441 tcaaccccat cgtcgctgaa caggatgacc tcgatcacca gatcctcttc gatatccacc
     1501 gcaaggcgac cgcagccgcg atcaagaaag ccatgcgcaa tgagcctagc atcgactggt
     1561 tactggagaa tcaggaaaag atcgtccaca agtatttcca gatgggattg gacggcaaga
     1621 tctgacggcg ttacaaccta catcagatta tcaagatgaa tctctaataa actgattaat
     1681 ataagtaact atcgtctcat tcaagcctgt atccaggcag tccaattcca cgatatcgtc
     1741 catgccacgc aaccaggtca gctgccgttt cgccagctgg cgcgtggcgg cgataccttt
     1801 ctcccgtagc gcgtccatat cgaattcgcc atccaggtat tgccacgcct gtcggtagcc
     1861 cacacagcgc atcgccggca gccctagatg caatgtcggg tatttcacgc ggagcgcacg
     1921 tacctcgtcc agcaggcccg cttccagcat ctgttcgaag cgaagggcga tgcgcgcatg
     1981 caaggccttg cggtccgagg gcaccaaagc cagcttgagt aagcgatacg gcaaggcgga
     2041 ctgtgtcttc tcttccgcga agatctggct catgggtttg ccggacaagg catagacctc
     2101 cagcgcccgc tggatgcgct gcgcgtcggt ggtcttgagg cgtgcagcgg tttccggatc
     2161 gatcgccgcc agcttggcat gcatgcccgg ccaaccgacc ttgagcgctt cggcatcgag
     2221 cgccgcgcgc gtctccgggt cggcctcggg caagtcattc aatccgccct gcaaggcctt
     2281 gaaatacaac atggtaccgc ccaccagcac cggcaccttg ccgcgtgccg tgatctcggc
     2341 catgagcttc aatgcagcgg tgcggaactg ggcggcggaa tacgcgtcgg tgggatcgat
     2401 gatgtcaatc aggtggtgcg gggccagctt gagtgtcgcg gcatccggct tggcggtacc
     2461 gatatccatg tcgcgataga ccagtgccga atcaacgc
//
LOCUS       DATMTW010000032         5308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_271422, whole genome shotgun sequence.
ACCESSION   DATMTW010000032 DATMTW010000000
VERSION     DATMTW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271422"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VN084_00635"
     CDS             complement(<1..1194)
                     /locus_tag="VN084_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWT27986.1"
                     /translation="MHSSSQIHGAIKSVRLPERERIPEDQASQAPWVAYDTPATWSGF
                     SSLIAAGRWESLVAIDGMRCAACAIGIEQLLQALPGVDAAEVSATSGKARVVWRAEAT
                     RPSAWLMAIAAAGYAPMPAEPDSGAQRQAQRRLLWRWLVAGFCMMQVMMYAYPVYVAE
                     PGEIGPDLLQLLRWAGWVLTLPVLLFSSAPFLKHAWQDLKHLRVSMELPVAIGILITF
                     AVSSAATWEPGGWWGREVYFDSLAMFVFFLLTGRWLEARLRDRTAGALDALCRRIPQT
                     ASRRTAEGDFVRVALDTLQVGDIVRVLPGEAFPGDGIILGGSTSVNEALITGESAPVP
                     KPQGSRVIAGSDNLSASVTVALHAVGTQTRYAEIVALMTRAAVDKPRLALLADRIARP
                     FLIFVL"
     gene            complement(1206..1649)
                     /locus_tag="VN084_00640"
     CDS             complement(1206..1649)
                     /locus_tag="VN084_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWT27987.1"
                     /translation="MAIPRSYKVEQLLVPVDGSASSLNALQYAAEMLKGRPQGMVHLI
                     NIQPLILPLGEYPDFDTLEKAQRHYAHGIIKQARQALAGADIAVATHIEIGPVAEGII
                     QYAQTHKIDQIVMGTRGMGALGNLVLGSVASRIVHLANLPVTLVK"
     gene            complement(1668..1976)
                     /locus_tag="VN084_00645"
     CDS             complement(1668..1976)
                     /locus_tag="VN084_00645"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWT27988.1"
                     /translation="MSTIHEMLIANKPIANKPIDGTAKTGASAKRRSRAKAAGRHVFS
                     NSEAHVTETDRQQMIADNAYFRAERRDFAPGSEMVDWLEADAEIDRYLDRNPGRQAIN
                     "
     gene            complement(2054..2473)
                     /locus_tag="VN084_00650"
     CDS             complement(2054..2473)
                     /locus_tag="VN084_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWT27989.1"
                     /translation="MTKLTKRRYVGLDDLFDHFMLQPVPNLASHNGLSIRIDVEEDDK
                     AFRVKAEIPGVKKEDIEVKINGNQLFIRAEVKSRHDEKQNNKIVRRERYHGEVSRSFS
                     FDEDIDAAGAEAKYEDGVLTLTLPKARDSRLKQLKVR"
     gene            2647..4008
                     /locus_tag="VN084_00655"
     CDS             2647..4008
                     /locus_tag="VN084_00655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWT27990.1"
                     /translation="MKLQFLGATGTVTGSKYLLQNQSGHTLVDCGLFQGFKQLRLRNW
                     SALPIAPARIDRVILTHAHIDHSGYLPLLVRQGFSGPVYCTSATRDLCEILLMDSAKL
                     QEEEADFANRHSFSKHKPALPLYDRHDAQRALRQLKVVEMGHKLTLDADTAFHFEPSG
                     HILGSAFICLESEGRHIVFSGDLGRPHDLVMSAPSIIQRADYLLIESTYGNRSHAQTD
                     VLEQLAEVINRTHHRGGKVIIPTFAVGRTQSLLYCFHLLKQRGMIDAALPIYLNSPMA
                     QEATRIFQQHPGEYRLTEKEVEGMCRDVKVITDIEESKWINTVKTPMILMAGSGMATG
                     GRIVHHLKQFAPDPKNTILFTGFQAGGTRGAAMLDGMKEIKIHGEYYPVNAEIALVDN
                     LSAHADASEIIGWLSHFQKPPRRTFITHGEPIAADALRMRIEEQLQWEIYLPDYMESV
                     ELA"
     gene            complement(4037..4765)
                     /locus_tag="VN084_00660"
     CDS             complement(4037..4765)
                     /locus_tag="VN084_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HWT27991.1"
                     /translation="MFADIATLGLSFVAGLLSTLSPCVLPLIPILVGAALASHPLGPW
                     GLGTGLALSFTVVGVLLASLGASLGIDQTLLREIGAVVLILLGATLLFPPLHQRFIML
                     TSGFGNAGQGLAAKISGDTLGGQFALGLLLGIIWTPCVGPTLGAAITLASQGESLAYV
                     TTVMLVFGLGAAIPLVVIGLLSRSVLGKVRGRLAKAGDIGKKVLGGLLLLLGLLIITG
                     ADKQLEALALDHLPASLLSFSTSI"
     gene            complement(4770..5150)
                     /locus_tag="VN084_00665"
     CDS             complement(4770..5150)
                     /locus_tag="VN084_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWT27992.1"
                     /translation="MKRWIMVLAALAATLQFTLAQAAVAYTQADFDKLMAEGKPVLVE
                     VHADWCSTCRTQAPITDALLKKPELSKVTALRVDFDKQKDTVKAFNVRQQSTLIVFKG
                     GKEVGRSIADTSASGIEALIRKAL"
BASE COUNT         1048 a         1715 c         1601 g          944 t
ORIGIN      
        1 cagcacgaag atcaggaacg ggcgcgcgat gcgatcggcg agcaatgcga gtcgcggctt
       61 gtctaccgcc gcccgtgtca tcagcgcgac gatctcggca tagcgggtct gcgtgcccac
      121 cgcgtgcagg gccaccgtca ccgaagccga caggttgtca ctgccggcga tgacgcggct
      181 accctgcggc ttgggcacgg gtgccgattc gccggtgatc agcgcctcgt tgacgctggt
      241 gctaccgccg agaatgatgc cgtcgcccgg aaaggcttcg cccggcaaca cgcggacgat
      301 gtccccgacc tgcaaggtat cgagcgcgac ccgcacgaaa tcgccctcgg cggtacgtcg
      361 gctcgccgtt tgcggtatcc ggcgacagag cgcgtcgagc gcgccagccg tgcggtcgcg
      421 caggcgcgct tcgagccagc ggccggtgag caaaaagaag acgaacatgg cgagcgaatc
      481 gaaatagacc tccctgcccc accagccgcc gggttcccag gtggcggccg aactcacggc
      541 aaaggtgatg aggatgccga tggcgaccgg cagctccatg ctcacccgca agtgcttgag
      601 gtcctgccag gcgtgcttga ggaatggcgc gctggagaac agcaacacgg gcagcgtcag
      661 cacccagcct gcccagcgca gcagctgcag caagtcaggc ccgatctcgc cgggctcggc
      721 cacatagacc gggtaggcgt acatcatcac ctgcatcatg cagaagcctg ccaccagcca
      781 gcgccagagc aggcgtcgtt gcgcctggcg ttgcgctcca ctgtccggtt cggccggcat
      841 cggcgcataa ccggccgcgg cgatcgccat cagccatgcc gaaggccggg tggcctctgc
      901 ccgccaaacc acccgtgcct tgccactggt ggcgctgact tcggcggcat cgacgcccgg
      961 cagcgcctgc agtagctgtt cgataccgat ggcgcaggca gcgcagcgca tgccgtcgat
     1021 ggcgaccagc gattcccatc gcccagccgc gatcaaactg ctgaatccgg accaggtcgc
     1081 cggggtgtca tatgcgaccc agggcgcttg actggcctgg tcttcgggga tgcgctcccg
     1141 ctctggcagc cgaacgcttt ttatggcgcc gtgaatttga cttgaggaat gcatgggctc
     1201 tgcgatcact tgaccagggt caccggcaga ttcgccaggt gcacgatcct gctcgccacc
     1261 gaacccagca ccaggttgcc caatgcgccc atgccacggg tgcccatgac gatctggtcg
     1321 atcttgtgtg tttgcgcgta ctggatgatg ccttcggcca ctgggccgat ctcgatatgc
     1381 gtggcgacag cgatgtccgc cccggccagt gcctgccggg cctgcttgat gatgccgtgg
     1441 gcgtaatggc gctgggcttt ttccagcgta tcgaaatccg ggtattcgcc cagcggcagg
     1501 atcaggggct ggatgttgat gagatggacc atgccctgcg ggcggccctt cagcatctcc
     1561 gccgcgtatt gcaaggcgtt gagcgaggac gcggaaccat ccaccggcac cagcagttgt
     1621 tcgaccttat agctgcgagg gatcgccatc ggagactttg ctgacgatca gttgatggcc
     1681 tggcggccag ggttacgatc gaggtaacgg tcgatctcgg catcggcttc gagccagtcg
     1741 accatctccg agccaggcgc gaaatcacgg cgctcggcac gaaaataggc attgtcggcg
     1801 atcatctgct gacgatccgt ttcggtgaca tgcgcctcgc tgttggagaa cacatgacgg
     1861 ccagcagcct tggcgcgact gcgccgcttg gcggatgcgc ctgttttggc tgtgccatca
     1921 atcggcttat tggcgatcgg tttgttggcg atcagcattt catggatcgt ggacatggtg
     1981 tactccttgt gcagtcaggt tggatcggtg gttcgggccg acatcaagcg tgtcggtcgt
     2041 caccccacgg ggttcagcgc accttgagct gcttcaggcg cgaatcgcgc gccttgggca
     2101 gcgtcagcgt caatacgccg tcttcgtact tggcttcggc gccagccgca tcgatatcct
     2161 catcgaagct gaaactacgg ctgacctcgc cgtgatagcg ctcgcgccga acgatcttgt
     2221 tgttctgttt ctcgtcatgc cgcgatttga cttccgcgcg gatgaacagc tggttgccgt
     2281 tgatcttgac ctcgatatcc tctttcttga cgccggggat ctcggccttg acgcggaatg
     2341 ccttgtcgtc ctcttccaca tcgatgcgga tcgacaagcc attgtgggag gcaaggttcg
     2401 gcactggctg gagcatgaaa tgatcgaaca ggtcatcaag acccacataa cgacgtttgg
     2461 tcagcttggt cattccagtc tcctctatcc gaaaatacga atccgagccg acagcatgcg
     2521 tcagccgcgg acaacagact ttgatccagg tcaaacaaca gtgggctaag gcgcatcgcc
     2581 agaaccggcg cggcaggatt cggtatagac tgggcatctc agccttggca tgggaacgac
     2641 ccgacgatga agctgcaatt cctcggtgcg accggcaccg tcaccggttc caaatacctg
     2701 ctgcagaacc agtccggcca cacgctggtc gattgcggcc tgttccaggg gttcaagcaa
     2761 ctgcggctgc gcaactggag cgccctgccc atcgcgccgg cccgcatcga tcgcgtcatc
     2821 ctcacccatg cgcatatcga tcacagcggc tacctgccgc tactggtgcg gcaagggttc
     2881 agcgggccgg tgtattgcac ctcggccacg cgcgacctgt gcgagatcct gctcatggac
     2941 agtgccaagc tgcaggagga agaagccgat ttcgccaacc gccacagctt ttccaagcac
     3001 aagcctgcct tgccgctcta tgaccggcat gacgcgcaac gtgcgctgcg gcagctgaaa
     3061 gtggtggaga tgggtcacaa gctgacgctg gatgccgata ccgcgttcca tttcgagcct
     3121 tccggccata tcctcggctc ggccttcatc tgcctcgaaa gcgagggccg gcacatcgta
     3181 ttctctggcg acctcggtcg cccgcatgat ctagtcatga gcgcgcccag tatcatccag
     3241 cgtgcagatt acctgctcat cgaatccacc tacggcaacc gcagccacgc ccagaccgat
     3301 gtgctggaac aactggcgga ggtcatcaac cgtacgcatc atcgcggcgg caaggtcatc
     3361 atccccacgt tcgctgtcgg ccgcacgcaa tcgctgctct actgcttcca cctgctgaag
     3421 caacgcggca tgatcgacgc tgcactgccg atctacctca atagcccgat ggcacaggag
     3481 gcgacgcgca tcttccagca acatcccggc gaataccgcc tgaccgaaaa ggaagtggaa
     3541 ggcatgtgcc gcgatgtgaa ggtcatcacg gatatcgagg aatcgaaatg gatcaacacc
     3601 gtgaagacac cgatgatcct catggctggc agcggcatgg cgaccggcgg ccgcatcgtg
     3661 catcacctga agcaattcgc gccagacccg aagaacacca tcctcttcac cggcttccag
     3721 gcaggcggca cgcgcggcgc ggccatgctc gacggcatga aggagatcaa gatccacggc
     3781 gagtactacc cggtcaacgc cgagatcgca ctggtagaca acctctcggc ccacgccgat
     3841 gccagcgaga tcattggctg gttaagtcac ttccagaaac cgccacgccg cactttcatc
     3901 acgcacggcg aaccgatcgc cgccgacgcg ctacgcatgc gcatcgagga acagttgcag
     3961 tgggagatct acctgccgga ctacatggaa tcggtcgagc tggcctgagc acggtcgcgc
     4021 ccggccagcc gaccggttag atcgaagtcg agaaactcag cagcgatgcc ggcaagtgat
     4081 ccagcgccag ggcttcgagc tgtttatccg caccggtgat tatcagcaga cccagcaaca
     4141 gcaacaggcc accgagcacc ttcttgccga tatcgccggc cttggccagt cggccgcgta
     4201 ccttgcccag cacgctacgc gacaacaagc cgatcaccac cagcgggatc gccgcgccca
     4261 aaccgaacac cagcatcacc gtcgtcacat aggccaggct ctcgccctgg ctcgcgagcg
     4321 tgatggcagc ccccaaggtc ggcccgacac aaggtgtcca gatgatcccg agcagcaggc
     4381 cgagtgcgaa ctggccaccc agggtatcac cggagatctt cgccgccagc ccctgcccgg
     4441 cgttgccgaa acccgaggtc agcatgatga agcgctggtg cagcggcggg aatagcaagg
     4501 tagccccaag caggatcagc accaccgcgc cgatctcgcg cagcaaggtc tggtcgatgc
     4561 cgaggctggc gccgagcgac gccagcagta cgccaaccac ggtgaaggac agcgcgaggc
     4621 cggtgcccag cccccacgga cccaggggat ggctggccag cgcggcgccg accaggatgg
     4681 ggatgagcgg cagcacgcat ggcgacaacg tggagagcaa cccggcgacg aaactcaggc
     4741 ccagcgtagc gatatcggcg aacatgggct tacagcgcct tgcggatcag cgcttcgatg
     4801 ccgctggcag aggtatcggc gatgctacga ccgacttcct tgccaccctt gaatacgatg
     4861 agcgtgctct gctgacgcac attgaacgcc ttgaccgtat ccttctgctt gtcgaaatcg
     4921 acacgcagcg ccgtcacttt cgacagctca ggctttttca gtagcgcgtc ggtgatcggc
     4981 gcctgtgtgc ggcaggtcga gcaccagtcg gcatgcactt cgaccagcac cggtttgcct
     5041 tcggccatca gcttgtcgaa atcggcttgc gtgtaggcga ctgcggcctg ggccagcgtg
     5101 aattgcaacg tggccgccag ggccgccaaa accatgatcc agcgtttcat ttccatctcc
     5161 ttgcgtgaac tatcgaataa gtggcttccg cgatatcgaa accgcgccgg ggcgccgtcg
     5221 ggcagacccc atacccatta gacggacagg cagcaccagc cttacagcac ggccaaaata
     5281 ttccggcact gaacttggaa ctgaactc
//
LOCUS       DATMTW010000033         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_607476, whole genome shotgun sequence.
ACCESSION   DATMTW010000033 DATMTW010000000
VERSION     DATMTW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607476"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..485
                     /locus_tag="VN084_00670"
     CDS             105..485
                     /locus_tag="VN084_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27993.1"
                     /translation="MKLLQVAEQAYRTLVEEQDDTILWLSQSMQKAGASMDLLLAGNC
                     AYYATVTQKQPALTLGAWQQQAPADIKRDLDQLQTQGAGIYVVHSDLVDRGLIGLPLH
                     PGVKVLDDEGVAQLYEDADKVWQW"
     gene            479..964
                     /locus_tag="VN084_00675"
     CDS             479..964
                     /locus_tag="VN084_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT27994.1"
                     /translation="MVAITHLPMPELAAAGTSAPLTLADIEKLSTFRTGLRQYLQHSH
                     LFCEIFDLTDLQYVFLLTVKAFDAQHQAAYRALKAQLSLTDSALTKLVRRSLGSDLLT
                     IVPAPVGETSQCFRLSAAGDRLIATLAALHKKEFESFQTRFTRQCDTHCESPICWKGS
                     D"
     gene            1016..1486
                     /locus_tag="VN084_00680"
     CDS             1016..1486
                     /locus_tag="VN084_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWT27995.1"
                     /translation="MHLTRFSDIGLRILMYLAREQREAPRVTITEISTQFGLPRNHVV
                     KITSRLARQGWVEATRGRSGGLRLGQAADQIRIGQVIKTLEPRDELIDCEGIGCRLSP
                     ACTLRSLLAQGLEAFYGHLDGYTLADLVSGDTGVEIVNMHRQFLQTAVEPGVPV"
     gene            1590..2789
                     /locus_tag="VN084_00685"
     CDS             1590..2789
                     /locus_tag="VN084_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HWT27996.1"
                     /translation="MLSNEAKSYIHASVPVLREHGNAITKVFYENLFTSHPELKHVFN
                     MGNQANGAQQQSLAAAVFAYASNIDQLENLTPVVRRIAHKHASLGIKPEQYPIVGQHL
                     LGAIKQTLGEAATTPLLQAWEQAYGMLADTLISFEKTITEDKGKPLGQMLRVRVSGIS
                     IQSELVKSFQLTPVDAPLPHFHPGQYVSVLARFADGTRQLRQYSLSDSTDKPYLRISV
                     KRETGSTTPDGQVSNWLHHEMKVGDEIEISQPYGDFTPDIMSDQPIILVSAGIGITPM
                     ISTLNEIAARNPSKRVIFAHLARRRAQHAHIDDLLNAFETMEQLDAVTFYKSPGPNDN
                     LGAYTHAGNFDVSKLPKFDIGKTSIFICGPSQFMKEIIRVFSALGVPRINIHREVFGP
                     DLLDLYL"
     gene            complement(2848..>3671)
                     /locus_tag="VN084_00690"
     CDS             complement(2848..>3671)
                     /locus_tag="VN084_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT27997.1"
                     /translation="RLFPHLSLKGNLGYGMRLTPPALRRLRLEDVVPLLELGPLLEQR
                     PGQLSGGERQRVALGRALLTSPELLLLDEPMASLDERLKAQILPFLRRVKEETAIPMV
                     YVSHAIGEVLALTQQIAVMQDGAILASGDYHEVLVSEHVLPLAQSLGIENLLPVRIRR
                     HHAALGYSEGDCCGHPLVLPLADAPVGAELTVVVQAANLSLARGAVVGTTIQNQVRGL
                     VTQVRLVEHRALVSLDIGTSLVVEVSAKSVQDLAIAPGQEVHCLIKTQSLRYLGR"
BASE COUNT          756 a         1164 c         1083 g          668 t
ORIGIN      
        1 ggcgtcaatg ccgccatcga cggctgccag cgcggttcgc ccgccgatct ctggcgttac
       61 gcgcaatctt ccgacaacac gctcgtcata gggacccgtt aaccatgaaa cttctacaag
      121 tcgccgaaca ggcctaccgc acattggtgg aggaacagga cgacaccatc ctctggctat
      181 cgcaatcgat gcagaaagca ggcgcgtcca tggacctgct gctggccggc aattgcgcct
      241 attacgccac cgtgacgcaa aagcagccgg cattgacgct gggggcatgg caacagcaag
      301 cgccagccga catcaagcgc gacctcgatc agttgcaaac gcaaggcgcc ggcatctatg
      361 tcgtccattc agacctcgtc gatcgtggcc ttatcgggct gcccttgcat ccgggtgtca
      421 aggtgctcga tgacgaaggc gtcgcccagc tctacgagga tgcggacaag gtgtggcaat
      481 ggtagcgatc acccatctgc cgatgccgga gttggcagcc gctggtacct ccgcgccact
      541 cacgttggcc gacatcgaga aactgtcgac cttccgcacc ggcttgcgcc agtacctgca
      601 gcacagccac ctgttctgcg agatcttcga tctcacagac ctgcagtatg tcttcctgct
      661 gacggtcaag gcgttcgacg cgcagcatca ggccgcctac cgcgcgctca aggcgcagct
      721 gtcattgacc gattccgcgt tgacgaagct ggtccggcgt tccctgggat ccgatctgct
      781 gaccatagta cccgcccctg tcggcgagac cagtcaatgc ttcaggctca gcgccgctgg
      841 tgaccgtctc atcgcgaccc tggcagcact gcacaaaaag gagttcgaga gttttcagac
      901 gcgctttacg cgtcagtgcg acacccattg tgaatcgccg atctgttgga aaggcagtga
      961 ttgatccggc atttcggttt aaaatcgcat tttttatgcg actttggact acgccatgca
     1021 tctgacccgg ttctccgata tcggcctgcg catcctgatg tacctcgctc gcgaacagcg
     1081 cgaagcgccc agggtgacga tcaccgagat ctcgacgcaa ttcggtttgc cacgcaacca
     1141 tgtcgtcaag atcaccagcc ggctcgcgcg gcaaggctgg gtggaagcga cccgtggccg
     1201 tagcggcgga ctccggctcg ggcaggcggc agaccagatc cgcatcggcc aggtgatcaa
     1261 aacgctggaa ccgcgcgatg aactgatcga ctgcgaaggc atcggctgcc gcttgagccc
     1321 tgcctgtacc ttgcgttcgt tgctggcgca gggactggaa gccttctatg gccacctcga
     1381 cggctacacg ctggcggacc tggtctcggg cgacaccggt gtcgagatcg tcaacatgca
     1441 tcggcagttc ctgcaaaccg ctgtcgagcc aggcgtgccg gtctgagcgg ccgggggatg
     1501 cagtgcaggg agccatgagc aaacgttttt tgagctgtgt tttttttaaa ataaaagttg
     1561 ctttttatat actactttga ggagatctta tgctgtcgaa tgaagccaaa tcctacatcc
     1621 atgccagcgt gccggtcctg cgcgagcacg gcaacgccat caccaaggtg ttctacgaga
     1681 acctgttcac cagccatccc gagctgaaac atgtgttcaa catgggcaac caggccaacg
     1741 gcgcgcagca gcaatcgctg gccgccgctg tcttcgccta cgcctccaac atcgaccagc
     1801 tggagaacct gacgccggtg gtacgacgga tcgcccacaa gcatgcgtcg ctggggatca
     1861 agcccgagca atatccgatc gtcggccagc atctgctggg tgcgatcaag cagacactgg
     1921 gcgaagccgc cacaacgccc ttactgcaag cctgggagca agcctacggc atgctggcgg
     1981 ataccctgat ctcgttcgag aagaccatca ccgaggacaa gggcaaaccg ctgggccaga
     2041 tgctgcgcgt gcgggtgagc ggtatcagca tccagagcga gttggtcaaa tccttccagc
     2101 tgacgccagt cgacgcgcca ctgccgcatt tccacccggg gcaatacgtc tccgtgctgg
     2161 cgcggtttgc cgacggcacc cgccaactgc ggcagtacag cctgtccgat tcgaccgaca
     2221 aaccctatct gcgtatctcg gtgaagcgcg agaccggaag cacgacgccg gatggccagg
     2281 tatcgaactg gttgcatcat gaaatgaagg tcggcgacga gatcgagatc agccagccgt
     2341 atggcgactt cacgccggat atcatgagcg accagccgat catcctcgtc tcagccggga
     2401 tcggcatcac gcccatgatc tcgacgctga acgagatcgc cgcgaggaac ccttccaagc
     2461 gcgtgatctt cgcccacctc gcccggcgcc gcgcgcaaca tgcccatatc gacgacctgc
     2521 tcaacgcctt cgagaccatg gaacagctcg atgcggtgac cttctacaag tcgccagggc
     2581 ccaacgacaa cctcggcgcc tatacccatg cgggcaattt cgacgtcagc aagctgccga
     2641 aattcgatat cggcaagacc tcgatcttca tctgcggccc gagccagttc atgaaagaga
     2701 tcatccgggt gttcagtgcg ctgggcgtgc cgcgcatcaa tatccatcgc gaggtgttcg
     2761 gaccggatct gctcgatctt tatctatgag catggcagcg cagtaaacgc caggaaggca
     2821 aacgccaaac gagtcggtag gccgcgctca gcggccgagg tagcgcagcg attgggtctt
     2881 gatcaggcaa tgcacctcct ggccgggcgc gatggccaga tcctgcacgg atttggccga
     2941 gacctcgacc acgaggctgg tgccgatatc gagactgacc agggcacggt gctcaaccag
     3001 acgaacctgg gtgaccagac ctcgcacctg gttctggata gtggtgccga cgacagcgcc
     3061 acgcgccagc gagaggttcg ctgcctgcac gaccaccgtg agctcggccc ctacaggcgc
     3121 gtctgcgagc ggcaatacaa gggggtggcc gcagcaatcg ccttcgctgt agccaagcgc
     3181 ggcgtgatgc ctgcggatac gcaccggcag cagattctcg atgcccagtg attgcgccag
     3241 cggcaacaca tgctcactga ccaggacttc gtgatagtcg ccactggcga ggatcgcgcc
     3301 gtcctgcatc acggcgatct gctgggtcag cgccagcacc tcgccgatgg cgtggctgac
     3361 atagaccatg gggatcgccg tctcttcttt cacccgccgc aggaatggca ggatctgcgc
     3421 tttcagtcgc tcgtcgaggg aggccatggg ttcgtccagc agcagcaact cgggcgaagt
     3481 cagcaaggca cgtccgagcg ccacgcgctg gcgttcgccg cctgagagct ggccaggccg
     3541 ctgctcgagc agtgggccaa gttcgagcag gggcaccaca tcctccagac gcagcctgcg
     3601 cagtgcgggc ggcgtcaatc gcatgccgta acccagattg cccttgagcg acagatgcgg
     3661 gaacagccgg c
//
LOCUS       DATMTW010000034         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_104311, whole genome shotgun sequence.
ACCESSION   DATMTW010000034 DATMTW010000000
VERSION     DATMTW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104311"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VN084_00695"
     CDS             <1..674
                     /locus_tag="VN084_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWT27998.1"
                     /translation="ASIPWILEHGGLAFQELGVPNSGGTKIFSVSGHIEKPGNYEVRM
                     GMPFTELLEMVGGIWKGRKLKAVIPGGPSTAVMPASAIMGATLDYDGLSKAGSSLGAG
                     SMIIMDETTCMVKTLKRLSYFFFEESCGQCTPCREGTGWLYRVINRITNGQGRMEDLD
                     LLTDVSGNISGRTICALGDAAATPVLSFIKHFRPEFEYYIQHGRSMVEDGVTMESLQK
                     ETVEA"
     gene            674..3091
                     /gene="nuoG"
                     /locus_tag="VN084_00700"
     CDS             674..3091
                     /gene="nuoG"
                     /locus_tag="VN084_00700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWT27999.1"
                     /translation="MLKVEIDGKPVEVDHGTTIIDAADQLGIAIPRFCYHKKLSVAAN
                     CRMCLVQVEKFAKPLPACATPVADGMKIFTRSKAAVEAQKSVMEFLLINHPLDCPICD
                     QGGECDLQDIAVAYGAPASRYAEEKRVVLNKNIGPLVSTDMTRCIQCTRCVRFLKEVG
                     GMMELGLINRGEHAEITAYVDKSVDSELSGNIIDLCPVGALTSKPFRYSARSWELTRR
                     PSIAPHDGLGSQIEVHVKDNKVMRVLPREKESINECWLSDRDRFSYEGLNSADRLKVP
                     MIKHNGAWQETDWKTALEFAAGQFKDITNEHGGEALGVLVSPNSTMEEGYLAKKIAEG
                     LGSQNIDYRVRQTDFRLDGKRFGTPWMGCNIPDVEEMDRILVIGSNLRNEHPLLTHRI
                     RKAVANGAELSVISPLDNDPLMQIAHKVIVRPNDMVNVLAQVLKAMSGLQKLSLRLPT
                     SLNQLLEDIRVRDNTQAIAESLVGMGKLYDLISPKTGIFVGNIALNDPRFTEMFSLAE
                     AMAGISGAILGLIPAGANNTGQHLMGVMPGPQGMHARAMLEIPRKAYLLVNLEPELDC
                     HNGAAARRAMQQAECVVALTMYKSEALEDADVLLPIAPFTETSGTLMSMEGRVQSFAA
                     VTRPLGECRPGWKVLRVLGNTLGLSGFDFDSSEAVKNAIFGGEKPSAVVWRSLNNNLK
                     ELVEIEVKIKSDGLQRIGEVPQYQADPIVRRSPPLQKTKYNLVPMARMHSKQAEALGL
                     AAGDSVLVKQGEGSAILKVRIDNHVAMGCVRIQAASPVTASLGELMGDLTVEKIAVEQ
                     AGSEAVA"
     gene            3088..4131
                     /gene="nuoH"
                     /locus_tag="VN084_00705"
     CDS             3088..4131
                     /gene="nuoH"
                     /locus_tag="VN084_00705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWT28000.1"
                     /translation="MIDFFAQLFGGYWPQVQLTAWTLIKIVAIVAPLMGAVAYLTLAE
                     RKVIGFMQVRIGPNRVGYFGLLQPIADGLKLLTKEIILPTASNKALFLLGPILAIAPA
                     FAAWAVVPFDAELILADVDAGLLYILAMTSVAVYGVIIAGWASNSKYAFLGSLRSAAQ
                     IVSYEIAMGFALVGVLMSAGSLNLGKIVVAQAGGLHQWYLWPLFPLFIVYFISAVAET
                     NRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMMLVSVLAAVMFLGGWQPLFD
                     VAPFTWIPGFVWLLLKVGFLLFCFLWFRATFPRYRYDQIMRLGWKVFIPITLVWIVVV
                     GGMMQTPWAYLFH"
     gene            4153..>4339
                     /locus_tag="VN084_00710"
     CDS             4153..>4339
                     /locus_tag="VN084_00710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985707.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWT28001.1"
                     /translation="MLRKIKNILSSLMLVELIKGLALTGRYFFARKITIQFPEEKTPQ
                     SPRFRGLHALRRYPNGEE"
BASE COUNT          852 a         1293 c         1304 g          890 t
ORIGIN      
        1 acgcttcaat cccctggatc ctggagcacg gtggtctggc tttccaggaa ttgggtgtgc
       61 ccaactctgg cggcaccaag atattctcgg tctctgggca tatcgagaaa cccggtaatt
      121 atgaagtgcg gatgggtatg ccattcaccg agctgctgga gatggtcggc ggtatctgga
      181 aaggtcgtaa gctcaaggcg gtgatccctg gaggtccatc gacggcagtg atgccggcct
      241 cggccatcat gggcgcgacc ctcgattacg atgggctttc caaggcgggt tccagtttgg
      301 gcgcaggttc catgatcatc atggacgaga ccacctgcat ggtgaagacg ctgaagcggt
      361 tgtcctattt cttctttgag gaatcctgcg gccagtgcac gccttgtcgt gaaggcacgg
      421 gctggctcta tcgcgtgatc aaccgcatca ccaatgggca agggcgcatg gaagacctcg
      481 atctcctgac cgatgtcagc ggcaacatat ccggtcgcac catctgtgca ctgggcgatg
      541 ccgcagcgac cccagtgctg agtttcatca agcatttccg gcctgagttc gagtactaca
      601 tccagcatgg ccgcagcatg gtcgaggatg gcgtgacgat ggaaagcctg cagaaagaaa
      661 cggtggaggc ctgatgttaa aagttgagat agacggcaag ccggtcgaag tcgatcacgg
      721 caccaccatc atcgatgcag ccgaccagct cggcattgcc atcccgcgtt tttgctacca
      781 caagaaactg tcggtcgcgg ccaactgccg catgtgcctg gtgcaggtcg agaagttcgc
      841 caaaccattg cctgcctgcg ccacgcctgt cgccgacggc atgaagatct tcacgcgttc
      901 caaggcagcg gtcgaggcgc agaagagtgt catggagttc ctgctcatca accatccgct
      961 cgattgcccg atctgcgatc agggtggcga gtgtgacctg caggatatcg ctgtcgctta
     1021 cggcgcaccc gcttcgcgct atgccgaaga gaaacgcgtg gtgctgaaca agaacatcgg
     1081 cccgctggta tccaccgaca tgacgcgctg tatccagtgc acgcgctgtg tgcgcttcct
     1141 caaggaagtc ggcggcatga tggagcttgg tctcatcaat cgcggcgagc acgccgagat
     1201 caccgcgtat gtcgacaaga gtgtcgattc cgagctttcc ggcaatatca tcgatctctg
     1261 cccggttggc gcattgacca gtaagccttt ccgttattcc gcacgcagct gggagctgac
     1321 acgccgtccc tcgatcgcgc cgcacgatgg gttgggctcg cagatcgaag tgcacgtcaa
     1381 ggacaacaag gtcatgcgcg tattgccgcg cgagaaggaa agcatcaacg aatgttggct
     1441 gtccgaccgt gaccgtttct cctacgaagg cctcaatagc gccgaccgcc tcaaggtgcc
     1501 gatgatcaag cacaacggcg cctggcagga aaccgattgg aagaccgcgc tggaattcgc
     1561 ggctggccag ttcaaggaca tcaccaacga acacggcggt gaagcgcttg gcgtgctggt
     1621 gtcgcctaac agcaccatgg aagagggtta cctggcgaag aagatcgccg aaggccttgg
     1681 ctcgcagaac atcgattacc gcgtacggca gaccgatttc cgtctggacg gcaaacgttt
     1741 cggcacgccc tggatgggct gcaatatccc tgatgtggaa gagatggacc gtatcctggt
     1801 catcggctcc aatctgcgca acgaacatcc gctgctgaca caccgcattc gcaaggccgt
     1861 cgccaacggc gccgaactca gtgtcatcag cccgctcgac aacgacccgt tgatgcagat
     1921 cgcgcacaag gtcatcgtac ggcccaacga tatggtcaat gtgctggcgc aagtactgaa
     1981 ggcgatgtcc ggcctgcaaa aactgtcgtt acggctaccg acctcgctca accagctgct
     2041 ggaagacatc cgcgtgcgcg acaacacgca ggccatcgcc gagagcctgg tgggtatggg
     2101 caagctgtat gacctgatct cgcccaagac gggcatcttc gtcggcaata tcgcgctcaa
     2161 tgatccgcgt ttcactgaga tgttcagcct ggcggaagcc atggccggca tcagtggcgc
     2221 catcctgggc ctcattcccg ccggcgccaa caatactggc cagcacctca tgggcgtgat
     2281 gccagggccg caaggcatgc atgcacgcgc gatgctggag attccgcgca aggcctatct
     2341 gctggtcaat ctcgagcccg aactcgactg ccataacggt gccgcggcac gccgcgccat
     2401 gcagcaggcg gaatgcgtgg tggcgctgac catgtacaaa tccgaagcgc tggaagacgc
     2461 cgacgtgctg ctgccgatcg cgccgttcac cgagacttcc ggcaccttga tgagcatgga
     2521 aggccgcgta cagagttttg ccgctgtgac ccgacccctg ggcgaatgcc gaccaggctg
     2581 gaaggtgctg cgtgtgctgg gcaatacgct aggtctgtct ggcttcgatt tcgacagtag
     2641 cgaagccgtc aagaatgcca tcttcggtgg cgagaagcca tctgccgtgg tctggcgcag
     2701 tcttaacaat aacctgaagg agttggtcga gatcgaggtc aagatcaagt ccgacggcct
     2761 gcaacgcatc ggcgaagtgc cacaatacca ggccgacccg atcgtgcgtc gttcgccacc
     2821 gctacagaag accaagtaca acctggtgcc gatggcgcgc atgcatagca agcaagccga
     2881 ggcgctcggc cttgccgcag gcgacagcgt gctggtgaag cagggcgaag gcagtgcgat
     2941 cctcaaggtg cgtatcgaca accatgtggc catgggctgc gtgcgcatcc aggccgccag
     3001 tcccgtgacc gccagcctcg gcgagctcat gggcgacctg acggtcgaga agatcgctgt
     3061 tgagcaagcc ggtagcgagg cggtcgcatg atcgacttct tcgcgcaact ctttggcggt
     3121 tactggccgc aggtgcagtt gacggcctgg acgctgatca agatcgtcgc catcgtcgcc
     3181 ccgttgatgg gggcggtcgc ctacctgacg ctggccgaac gcaaggtcat cggcttcatg
     3241 caggttcgta tcggcccgaa tcgcgtgggc tatttcggcc tgttgcaacc gatcgccgac
     3301 ggcctgaagt tgctgaccaa agagatcatc ctgccgacgg cgtcgaacaa ggcgctgttc
     3361 ctgctcggtc ccatccttgc catcgcgccc gcgttcgcgg cctgggcggt ggtgcctttt
     3421 gatgctgaac tcatactggc cgacgtcgat gccggtttgc tgtacatcct ggctatgacc
     3481 tcggtcgccg tttatggcgt catcatcgcc ggctgggctt ccaactccaa atatgcgttc
     3541 ctgggttcct tgcgttccgc ggcacagatc gtctcttacg agatcgccat gggcttcgcc
     3601 ctcgtcggcg tgctgatgag tgctggcagc ctcaatctgg gcaagatcgt ggtggcgcag
     3661 gccggcgggt tgcaccagtg gtacttgtgg cctttgttcc cgctgttcat cgtctatttc
     3721 atcagcgccg tggccgagac caaccgcgca ccgttcgacg ttgccgaagg cgagtcggag
     3781 atcgtcgccg gtttccatgt cgaatactcc ggtatggcgt tcgctgtatt cttcctggct
     3841 gaatacgcca acatgatgct ggtgtcggta ctggcggcgg tgatgttcct cggcggctgg
     3901 cagccgctgt tcgatgtcgc gcctttcacc tggatccccg gcttcgtctg gttgctgctc
     3961 aaggtcggtt tcctgctgtt ctgtttcctc tggttccgtg ccaccttccc acgctatcgc
     4021 tatgaccaga tcatgcgtct gggctggaag gtgttcatcc cgatcacgct ggtctggatc
     4081 gtcgtcgtcg gcggcatgat gcaaacgccg tgggcttacc tattccacta atattccact
     4141 aaggcccaag acatgttgcg caaaatcaag aacatattgt ccagcctgat gctggtggag
     4201 ctgatcaagg gcctggccct gaccggccgc tatttcttcg cacgcaagat caccatacag
     4261 ttcccggaag agaagacgcc gcaatctccg cgtttccgcg gcctgcatgc gctgcggcgc
     4321 tacccgaacg gcgaggaac
//
LOCUS       DATMTW010000035         5910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_271676, whole genome shotgun sequence.
ACCESSION   DATMTW010000035 DATMTW010000000
VERSION     DATMTW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271676"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1640)
                     /gene="groL"
                     /locus_tag="VN084_00715"
     CDS             complement(3..1640)
                     /gene="groL"
                     /locus_tag="VN084_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147112.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWT28002.1"
                     /translation="MAAKQVIFGDEARAKIVNGVNILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLENMGAQLVKEVASKTSDNAGDGTTTATVLAQAIVRE
                     GFKFVAAGFNPTDLKRGIDKAVVAIVEEIKKISQPTKTSKEIAQVGSISANSDADIGQ
                     IIADAMDKVGKKGVITVEDGSGLQNELDVVEGMQFDRGYLSPYFINNPDRQIALLDNP
                     FVLLYEKKISNIRDLLPALEQVAKAGRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDIAILTGGTVIAEDLGLTLEKVTLEDLGQAKRIEVGKEDTTIID
                     GAGNENNIKTRIEQINKQVEEASSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRARAAIKELKGDNPDQDAGIKIVLRAIEEP
                     LRQIVANAGDEPSVVVNKVLEGKGNYGYNAANGTYGDLVELGVLDPAKVTRSALQNAA
                     SVAGLMLTTDCMVAELPREDSPAMPGGMGGMGGMDMM"
     gene            complement(1687..1977)
                     /locus_tag="VN084_00720"
     CDS             complement(1687..1977)
                     /locus_tag="VN084_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWT28003.1"
                     /translation="MKIRPLHDRVIVKRLEAERKTASGIIIPDSATEKPDQGEVIAVG
                     PGKKDDNGKSIVLDVKVGDKVLFGKYAGQAVKVGNDEVLVMREEDIMGVVEA"
     gene            2141..2689
                     /locus_tag="VN084_00725"
     CDS             2141..2689
                     /locus_tag="VN084_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28004.1"
                     /translation="MAENALINASIQQLNLGYDGVEDRLLLRLGLSDNTEVKIWLTRR
                     MVKAVWGLLQSADLLPVTAPGMFATQTSEAPAKSATEPTAQAPMKRMDFSETYDDSRS
                     DLTGAPLLPRECRILALDNGQHVMELQTDGQMNLRIPMNQELIQALTNMLQITSKDAG
                     WDLSFAEGVTLIPESTNRPVLH"
     gene            2690..4000
                     /locus_tag="VN084_00730"
     CDS             2690..4000
                     /locus_tag="VN084_00730"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HWT28005.1"
                     /translation="MDNKTLLNRSLQAVWHPCTQMKHHETLPLIPIQRGDGIWLEDFD
                     GKRYLDGVSSWWVNLFGHNHPRIKQAIKDQLDQLEHVILAGFTHAPVVELSERLAALT
                     GLGHAFYASDGASATEIALKMSFHYWRNHNKPEKAGFISLQQSYHGETLGALSVTDVA
                     LFKDTYAPLLRHSTQVPSPDWRLAEAGESAEAYALRCAEALEAQVAHHHHETAALILE
                     PLVQCAAGMAMHHPAYLRRAREICDTYGVHLIADEIAVGFGRTGTLFACEQADIRPDF
                     MCLSKGITGGYLPLSVVLTTDAVYDAFYDDSTLRGFLHSHSYTGNPLACSAALATLDI
                     FDSEQVVAANIAKAAFLQQQMGQLADLPIRHLRQTGMIAAFDVETEDPHFARRFYQAA
                     LAKGLLLRPVGNTVYYMPPYIIDETQIAWMVERTCQVLREMALA"
     gene            3993..5420
                     /gene="dacB"
                     /locus_tag="VN084_00735"
     CDS             3993..5420
                     /gene="dacB"
                     /locus_tag="VN084_00735"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HWT28006.1"
                     /translation="MPRRAIGRAAAFLACLWPLLGHAQLPAPVSDALGKAGVPQEAVS
                     VIVQSVDETGGQPLRLVTHRPDQPMNPASTMKLLTTYAGLEILGPAYRWRTEIYRNGE
                     VVDGVLNGDLILKGYGDPELMAQDLWLMLGRLRQAGIREIHGDLVLDNTYFASTAPDA
                     AAFDNEPYRAYNATPGALVSNLKATSFRFLPDTQQLRILAEPDIPQITIINQVKWSPG
                     ACGDWKGRLSYAVANQGNRATVTFGGSYATACGEKVLELSVLNETAYTFDLFRSIWQS
                     LGGTLAGGMRAEATPEKAVLVFPYESASLSDVIRRINKYSNNLMARQLYLTIAAEQVA
                     IPGREPDGEKAIRAWLAGKGLQFPELVLENGAGLSRIERISADHMATLLQQAYDSPVM
                     PELMSSLPVLAVDGTTRKRLKDSAAHGRAHLKTGSLEGVRAIAGYLLDQHDRRWIVVF
                     MANHANAWATKAAQDALLDWVYHQP"
     gene            5506..>5910
                     /locus_tag="VN084_00740"
     CDS             5506..>5910
                     /locus_tag="VN084_00740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWT28007.1"
                     /translation="MKRATLYAGAALMAIFATSACTADSNTSNQETTAMTQNTNELVK
                     IDTVIGQGREAEPGFNVTVHYTGWLYDPNKPDHKGQKFDSSVDRNEPFVFFLGGGQVI
                     QGWDEGFAGMKVGGKRTLIIPPHMGYGEYGAGG"
BASE COUNT         1128 a         1773 c         1760 g         1249 t
ORIGIN      
        1 aattacatca tgtccatgcc gcccataccg cccatgccgc ccggcattgc cggactgtct
       61 tctctgggca gttcagcaac catgcagtcg gtggtcagca tcaagccggc cacggaagcc
      121 gcgttctgca gtgcggaacg ggtcaccttg gctgggtcca gtacgccaag ttcgaccagg
      181 tcgccgtagg tgccgttggc ggcgttgtaa ccgtagttac ctttgccttc cagaaccttg
      241 ttgacgacga cagaaggctc gtcaccagcg ttggcaacga tctgacgcag tggctcttcg
      301 atcgcgcgca gcacgatctt gataccggcg tcctggtccg ggttgtcacc cttcagttcc
      361 ttgatcgcgg cacgagcacg cagcagggca acgccaccac cggcgacaat gccttcttca
      421 accgcagcgc gggtcgcatg cagtgcatct tcgacgcggg ccttcttttc cttcatttcg
      481 acttcggtgg ctgcgccgac cttgatcacc gcaacaccac cagccagctt ggcgacacgt
      541 tcttgcagct tttcgcggtc atagtcgctg gaagcttctt cgacttgctt gttgatctgc
      601 tcgatacggg tcttgatgtt gttctcattg ccggcgccgt cgatgatggt ggtgtcttcc
      661 ttgcccactt cgatgcgctt ggcttggccg aggtcttcca gcgtgacctt ttccagggtc
      721 aggccgaggt cttcagcgat gacggtgccg ccagtgagga tggcgatatc ttccagcatg
      781 gccttgcgac ggtcgccgaa gcctggcgcc ttgacggcga cggtcttcag gatgccgcgg
      841 atgttgttga cgaccagggt cgccagcgct tcgccttcga cgtcttcagc gatgatcagc
      901 agcggacggc cagccttggc gacttgttcc agtgcaggca gcaggtcacg gatgttggag
      961 atcttctttt cgtacagcag cacgaaagga ttgtccagca gcgcgatctg acgatcggga
     1021 ttgttgatga agtaagggga gaggtagccg cggtcgaatt gcatgccttc gacgacgtcc
     1081 agttcgtttt gcaggccgga accgtcttca acggtgatca cgcccttctt gcccaccttg
     1141 tccatcgcat cggcgatgat ctggccgata tcggcgtcgg agtttgcgga gatggaaccg
     1201 acctgtgcga tctccttgct ggtcttggtc ggctgggaga tcttcttgat ctcttcgacg
     1261 atcgcaacga cagccttgtc gataccacgc ttgaggtcgg tcgggttaaa accggcagca
     1321 acgaacttga agccttcgcg cacgatggcc tgggccagca cggtggcggt ggtggtgccg
     1381 tcaccggcgt tgtcggaggt cttggaggcg acttccttca ccagttgcgc acccatgttc
     1441 tcgagtttgt ccttgagttc gatctccttg gccacggaca caccgtcctt ggtcacggtc
     1501 ggtgcgccga aggagcgctc gagcacgacg ttacggccct tgggccccag ggtcaccttg
     1561 acggcattcg ccaggatgtt gacgccgttg acgatcttgg cacgtgcctc atcgccgaaa
     1621 attacttgtt ttgcagccat ttgtatctcc tagagtctga ttctgtaatt tgatgacgag
     1681 agtgaattag gcctcgacga cgcccatgat gtcttcttcg cgcatgacca gcacttcgtc
     1741 attgccaacc ttgacggcct ggccggcgta tttgccgaac agcaccttgt cgccgacctt
     1801 gacgtcgagc acgatggact tgccgttgtc atccttcttg ccagggccga cggcgatcac
     1861 ttcgccttga tccggctttt cggtggcact gtctgggatg atgatgcctg atgcggtctt
     1921 gcgttctgct tcaaggcgct taacaataac gcgatcgtgt agaggacgga ttttcatgcg
     1981 gagttctcct taaattgggt tgattcacga acaaggtgtt acgaattctt gcgtaagggg
     2041 tgctgttagc actctgcctt cttgagtgct aataataagg gcgggacatg tatttttcaa
     2101 gtccgcccgc aggataaaga ttcatagact tggagtgtgc gtggcagaaa atgcattgat
     2161 caacgcttcc atccagcagc tcaacctggg ttacgacggc gtagaagacc ggctgctatt
     2221 gcggctgggg ctcagcgaca acaccgaagt caagatctgg ctgacgcgac gcatggtcaa
     2281 agccgtctgg ggcttgttac agagtgcaga cctgttacca gtgactgcgc ctgggatgtt
     2341 tgcaacccag accagcgaag cgcctgcaaa atcggcgacg gaacccacgg cgcaggcgcc
     2401 aatgaaacgc atggatttct ccgagaccta cgatgacagc cgtagcgatc tgaccggcgc
     2461 gcccttgctg ccgcgtgaat gtcgtatcct cgccctggac aatggccagc atgtcatgga
     2521 gctgcaaacc gacggccaga tgaacctgcg catccccatg aatcaggagc tgatccaggc
     2581 cctgaccaat atgttgcaga tcacgagcaa ggatgctggt tgggatctga gcttcgccga
     2641 gggggtcacg ctgatcccgg aatccaccaa ccgaccggta ttgcattaga tggacaacaa
     2701 gactttactg aaccgcagtc tgcaggcggt ctggcatcca tgcacgcaga tgaagcatca
     2761 cgagaccttg ccgttgatcc ctatccagcg cggtgacggc atctggcttg aagacttcga
     2821 cggcaaacgt tatctggacg gcgtcagctc ctggtgggtc aatctgttcg gacataacca
     2881 tccgcgtatc aagcaggcga tcaaggatca gctcgaccag ctggagcacg tcatcctggc
     2941 cgggttcacc catgcgccgg tggtcgaatt atccgaacgc ctggccgcgc tcacgggtct
     3001 cggccatgcc ttctatgcgt cggatggcgc ttcggcgacc gagatcgcgc tgaagatgag
     3061 tttccattac tggcgtaacc acaacaagcc ggagaaagcc ggcttcatca gcctgcagca
     3121 aagctatcac ggcgaaacgc tcggcgcgct gtcggtgact gacgtcgcct tgttcaagga
     3181 tacttacgcc cctttgctgc gccatagcac gcaagtgccc tcgccggact ggcggctggc
     3241 ggaggcgggc gaatctgctg aagcctatgc cttgcgctgt gccgaagcgc tagaagcgca
     3301 ggtggcgcat catcaccatg agacggccgc cctgattctt gaacccctgg tgcaatgtgc
     3361 cgccggcatg gccatgcatc acccggccta tctccggcgc gcgcgcgaga tctgcgacac
     3421 ctacggggtg cacctcatcg ccgacgagat cgcggtcggc ttcggtcgca ccgggacttt
     3481 gttcgcctgc gagcaggctg atatccggcc ggatttcatg tgcctgtcga aaggcatcac
     3541 gggcggctac ctgccgttgt cggtggtgct gaccaccgac gcggtatacg acgctttcta
     3601 tgacgacagc actttgcggg gtttcctgca ttcgcatagc tataccggca acccgctggc
     3661 ctgcagcgca gcgctggcaa cgctggatat cttcgatagc gagcaggtgg tcgccgccaa
     3721 catcgccaag gcggccttcc ttcagcaaca gatggggcag ctcgccgacc tgccgatccg
     3781 gcatctgcgg caaaccggca tgatcgccgc tttcgacgtc gagacagagg atccgcactt
     3841 tgcgcgccgg ttctaccagg ccgcgctggc gaagggcctg ctcttgcgcc ccgtcggcaa
     3901 cacggtctat tacatgccgc cctatatcat cgacgagaca cagatcgcct ggatggtcga
     3961 acggacttgc caggtgttgc gggagatggc gcttgcctag gcgtgccatc ggccgggcgg
     4021 ccgcgtttct ggcctgcctc tggcccctgc tcggacacgc acagttgccg gcgccggtga
     4081 gcgatgcact gggcaaggcc ggcgtgccgc aagaggcggt ctcggtcatc gtgcaatccg
     4141 tggatgaaac cggcggtcag ccgctacggc tggtcacgca ccgcccggat cagccaatga
     4201 atccggcctc gaccatgaag ttgctgacga cgtatgccgg cctggagatc ctgggcccgg
     4261 catatcgttg gcgtaccgag atctaccgca atggcgaggt ggtggatggt gtgctcaatg
     4321 gcgacttgat cctcaagggc tacggcgatc cggagctcat ggcgcaggat ctctggctca
     4381 tgttgggtcg tctgcgacag gctggtatcc gcgagattca tggcgacctg gtgctcgaca
     4441 atacctattt tgcgtccacg gcgcctgatg cggccgcctt cgacaatgag ccttaccgcg
     4501 cctataacgc cacgcctggc gcgctggtaa gcaatctcaa ggcgaccagc ttccgtttcc
     4561 tgcccgatac acagcagctc aggatcttgg ctgaacccga catcccccag atcacgatca
     4621 tcaaccaggt gaaatggtcg ccgggtgcct gtggcgattg gaaggggcga ctgagctacg
     4681 cggtcgcgaa ccagggcaac cgcgccaccg tcactttcgg tggcagttat gccaccgcct
     4741 gcggtgagaa ggtgctcgag ctcagcgtgc tcaatgagac cgcctataca ttcgacttgt
     4801 ttcgcagcat atggcaatcg ctaggcggca ccctggccgg tggcatgcgc gccgaagcca
     4861 cgcccgagaa ggcggtgctg gtgttcccct acgaatcggc gtcgctatcc gacgtgatcc
     4921 ggcgcatcaa caaatacagc aacaatctca tggcgcgcca gctctacctc accatcgccg
     4981 ctgaacaggt ggcgataccc ggccgggaac ctgatggcga aaaggcgatc cgggcatggc
     5041 ttgccgggaa ggggctgcag ttcccggaac tggtgctgga gaacggcgcc ggtctgtcgc
     5101 ggatcgagcg tatcagtgcc gaccatatgg ccacactgtt gcagcaggcc tacgatagcc
     5161 cggtgatgcc ggaattgatg tcatcgctgc cggtgctggc tgtcgatggg acaacgcgta
     5221 aacgcttgaa ggacagcgcc gcccatgggc gcgcgcacct gaagacaggc tcgctcgagg
     5281 gtgtacgtgc cattgccggt tatctgctgg atcagcacga ccggcgctgg atcgtggtct
     5341 tcatggccaa tcatgctaat gcctgggcga ccaaggcggc acaggacgcc ctgctcgact
     5401 gggtctatca ccagccctga gatcgagctg cgcacggaac tgcgccgcat ttccggtatc
     5461 atatttcagc tttgcactca ctcaaccctg cttttcggag cctttatgaa acgcgcaacc
     5521 ctctatgccg gtgccgcctt gatggcgatc ttcgcgacca gcgcctgcac cgctgattcc
     5581 aacacatcca atcaggaaac cactgcaatg acccagaaca ccaatgaact cgtcaagatc
     5641 gataccgtga tcggccaagg ccgcgaagcc gaaccgggat tcaacgtcac cgtgcactac
     5701 acgggctggc tctatgaccc aaacaagccg gaccacaagg gccaaaagtt cgacagctct
     5761 gtcgatcgca atgagccgtt cgtgttcttc ctcggtggcg gccaagtgat ccagggctgg
     5821 gatgaaggtt tcgctggcat gaaggtcggc ggcaagcgta cgctgatcat cccgccacac
     5881 atgggatacg gcgaatacgg tgcgggcggc
//
LOCUS       DATMTW010000036         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_37252, whole genome shotgun sequence.
ACCESSION   DATMTW010000036 DATMTW010000000
VERSION     DATMTW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37252"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1276)
                     /locus_tag="VN084_00745"
     CDS             complement(86..1276)
                     /locus_tag="VN084_00745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT28008.1"
                     /translation="MDTRPLPQLDYRSQILMPLIVVSCIMALAMGWVFDVAQDLIKAG
                     ERKVQIEQTRYRIGAILSAVVDAEAGQRGYLLTGRTQFLEPYDAAVRNTGKLRADLAL
                     AGQVFPDIAPQTQRIAGLIEQKLDIIRDSLNLERNAGAYAPHLRLAKDRGKPVMDEIR
                     LATASADAALVSESSRLERKIGSRSKQLALATLVILMLIVSILYWSYKRTIWLFEHAV
                     ENIKRAEKLGHIAMHDALTHLPNRRNFDEYLAKVHAQAARAQKQYALFYMDLDGFKLI
                     NDRFGHDVGDDALLATITRFGNILRESDYLARIGGDEFTLIVQDYKEQKELVILALRI
                     ISALDRPIMSIDGQDIWMGVSIGIATYPRHAQDINDLVASADGAMYQAKTSGKNQYCF
                     APQT"
BASE COUNT          278 a          410 c          481 g          336 t
ORIGIN      
        1 atgtgattcc ttttgggagt taagcgagta tgtgccctgg gtaagggcac atgggatcac
       61 atgataccaa ggggatgttt cagaatcatg tctgtggcgc gaagcagtac tggttcttgc
      121 cggaagtctt ggcctgatac atcgcaccgt cggcgctcgc caccaggtcg ttgatgtctt
      181 gcgcatgtct gggataggtg gcgatgccga tactcacgcc catccatatg tcttggccat
      241 cgatcgacat gatgggccgg tcgagtgcgc tgatgatgcg tagcgcgagg atcaccagct
      301 ccttctgttc cttgtagtcc tgcacgatga gcgtgaattc gtctccgccg atacgggcca
      361 gataatcgga ttcgcgcagg atattgccga agcgcgtgat ggtggcgagc agggcgtcgt
      421 cgccaacgtc gtgaccgaag cggtcgttga tcagcttgaa gccgtcgaga tccatgtaga
      481 acaacgcgta ctgtttctgg gcccgtgcgg cctgggcgtg gaccttggcc aggtattcgt
      541 cgaagttgcg gcggttcggc aggtgggtga gggcatcatg catggcgata tgaccgagct
      601 tttccgcgcg cttgatgttc tccactgcgt gttcgaacag ccagatcgtc cgtttgtagc
      661 tccagtaaag gatactgacg ataagcatca ggatgacgag cgtcgccaaa gccagctgct
      721 tcgatcggct gccgatcttg cgttcgagac ggctggactc gctcaccagc gcggcgtcag
      781 ccgaggccgt ggccagcctg atctcgtcca tcacgggctt gcccctgtcc ttggccaggc
      841 gcaggtgggg tgcataagcg ccggcattgc gctccaggtt gagcgagtca cggatgatgt
      901 cgagtttctg ctcgatcagg ccggcgatcc gctgcgtctg gggcgcgatg tcgggaaaga
      961 cctgacccgc gagcgcaaga tcggcgcgca gttttccggt attcctgacg gcggcatcgt
     1021 agggttcgag gaactgcgtg cggccggtca ggaggtagcc gcgctgcccc gcctcggcgt
     1081 cgaccacggc actgaggatc gcgccgatgc gatagcgggt ctgctcgatc tggactttac
     1141 gttcgccggc cttgatgagg tcctgggcga catcgaagac ccagcccatc gccagcgcca
     1201 tgatgcagga gacgacgatc agcggcatca ggatctggct gcggtagtcc aactgcggaa
     1261 gtggtctggt atccatggtc atttctgatc gtcttgctcg tcctgacgtg ctttcttcaa
     1321 atcgtgaccc catgcatcga tagctgctta tgcatagccc tttggggaag accgggataa
     1381 cgccagccta cgccctatcg gaccgtattt gaatcattaa gttgggggga agggatcagt
     1441 gtttggatcc tgctcgcgca ggaaccaaaa ggctcaggcc cggtcatgca catgtggccc
     1501 aaaat
//
LOCUS       DATMTW010000037         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_271832, whole genome shotgun sequence.
ACCESSION   DATMTW010000037 DATMTW010000000
VERSION     DATMTW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271832"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1945
                     /gene="hscA"
                     /locus_tag="VN084_00750"
     CDS             80..1945
                     /gene="hscA"
                     /locus_tag="VN084_00750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HWT28009.1"
                     /translation="MALLQIAEPGMSAAPHQHKLAIGIDLGTTNSLVATVRSGMSTVL
                     HDEQGRALLPSVVHYLADGVIDVGYKAQAQQSVDPQNTIVSVKRFMGRGLQDIADIAH
                     LPYHFIDTPGMVQLDTRAGIKSPVEVSAEILKVLKQRAEKSLGGELAGAVITVPAYFD
                     EAQRQATKDAARLAGLTVLRLLNEPTAAAVAYGLDNASEGVYVIYDLGGGTFDISILR
                     LSKGVFEVLATNGDSALGGDDFDRRIFCWVLEQTKLPPINDKDTRLLLTKAREAKEWL
                     SEHSEARITSVLGTGDVVDLTLTSDTLTELTRHLVTKTLTPTRKALRDAGLSTDEVKG
                     VVMVGGATRMPQIRQAVAEFFGQAPLTNLDPDKVVALGAAIQANVLAGNRSDDDLLLL
                     DVIPLSLGLETMGGLVEKVIPRNSTLPVARAQDFTTFKDGQTAMSIHVVQGERELVSD
                     CRSLARFELRGIPPMAAGAARIRVTFQVDADGLLSVTAREQTSGIESNIVVKPSYGLS
                     EDAITSMLQASFSSAESDKEARALREAQVDAQRLLEAIIAALQKDGALLSDDERAALE
                     REMVALAELAQGTDSQAIHKAVDRLNHATETFAARRMDASVKQALAGQNLNTLEL"
     gene            1948..2301
                     /gene="fdx"
                     /locus_tag="VN084_00755"
     CDS             1948..2301
                     /gene="fdx"
                     /locus_tag="VN084_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HWT28010.1"
                     /translation="MPQIIVLPHQELCPEGAAIDAKPGVSICETLLEHDIDIEHACDQ
                     ACACTTCHVIVREGFDSLNPAEDKEEDLLDRAWGLEPKSRLSCQAIVADRDLVVEIPK
                     YSINMAKEGMPKPKA"
     gene            complement(2418..>3176)
                     /locus_tag="VN084_00760"
     CDS             complement(2418..>3176)
                     /locus_tag="VN084_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HWT28011.1"
                     /translation="LFGNFAQSQYKGDRKDFSIDTSDSASNNLLAFQGGAKFDLTKTV
                     SAKAALTFTKFTNDHAPGASTFVPGLGNGTSLGTNPGATNDITTIEVPLELAFKLNDG
                     MKLTAFSDYVKNIDGDDRFDAAVAAGGANAAAIRAAGKDDKAWLLGVGFGKSQGKTAM
                     KGDWSAKIWYQDVGVYALDPNAVDSDFMDSRINMKGVVFKGEYLLRDNVYLNLAAGHG
                     SRKNDDLAAVGTGGDIALNLDSFNLYQADITYKF"
BASE COUNT          678 a         1019 c          931 g          548 t
ORIGIN      
        1 tggaagactg agcacaggcc acataaagcc aagcagaacg aacacttagc ccaaacccga
       61 accaacacca gaaaaagaca tggcattact ccagatcgca gaaccaggca tgagcgccgc
      121 gccacaccag cacaagctgg ccatcggcat cgacctcggc acgaccaatt cgctggtcgc
      181 gactgtgcgc agcggcatga gcaccgtgct gcacgatgag cagggtcgcg cgctgctgcc
      241 ctccgtcgtc cattacctgg cagacggcgt gatcgacgtc ggctacaagg cccaggccca
      301 gcagagcgtg gatccacaga acaccatcgt ctcggtcaag cgcttcatgg gccgcggcct
      361 gcaggatatc gccgacatcg cccacctgcc ctaccatttc atcgacacgc cgggcatggt
      421 gcaactcgac acccgcgctg gcatcaagag cccggtcgag gtctcggccg agatcctgaa
      481 agtcctgaag cagcgcgccg agaaatcgct gggcggcgaa ctcgcaggcg cggttatcac
      541 cgtgcccgct tacttcgacg aggcccaacg ccaggcaacc aaggatgcgg cccgactggc
      601 tggcctgacc gtacttcgcc tgctcaatga accgaccgcg gcggccgtgg catatggtct
      661 ggataacgcc tcagaaggcg tctacgtgat ctacgacctg ggtggcggca ccttcgatat
      721 ctccatcctg cggctctcaa aaggcgtgtt cgaggtgctc gcgaccaatg gcgactcagc
      781 gctgggcggc gacgatttcg accgccgcat cttctgctgg gtgctggaac agaccaagtt
      841 gccgccgatc aacgacaagg atacgcgtct actgctgacc aaggcgcgcg aagccaagga
      901 atggctcagc gagcatagcg aagcgcgcat caccagtgtg ctcggcaccg gcgacgtcgt
      961 cgacctcaca ctgaccagtg acaccctgac cgaactgacg cgccacctgg tcaccaagac
     1021 cctgacaccg acccgtaagg cactgcgaga tgcgggcctg tcgactgacg aggtcaaagg
     1081 cgtggtcatg gtcggcggcg cgacgcgcat gccgcagatc cgccaggccg tggccgaatt
     1141 cttcggccag gcgccgctga ccaatctcga cccggacaag gtggtcgcgc ttggcgccgc
     1201 catccaggcc aacgtgctgg ccggtaaccg cagcgacgac gatctgctgc tgctcgacgt
     1261 catcccacta tcgctgggac ttgagaccat gggtggactg gtggagaagg tcatcccgcg
     1321 caattccacc ttgccagtgg cacgcgccca ggatttcacc acgttcaagg atggccagac
     1381 agccatgagc atccatgtcg tccagggcga acgcgaactg gtatccgatt gccgctcgct
     1441 ggcacgcttc gaactacggg ggatccctcc gatggccgcc ggcgcagccc gtatccgcgt
     1501 caccttccag gtcgatgccg atggtttgct ctcggtcacg gcacgcgaac agaccagcgg
     1561 catcgagtcg aacatcgtgg tcaagccatc ctacggcctc agcgaagacg cgatcaccag
     1621 catgctgcaa gcctcgttca gcagcgccga gagcgacaag gaagcccgtg cgctgcgcga
     1681 agcccaggtg gatgcgcaac gtctgctgga ggccatcatc gccgccctgc agaaagacgg
     1741 cgccctgctc agcgatgacg agcgcgcggc cctcgaacgc gagatggtgg cgcttgctga
     1801 actagcccag ggcacggaca gccaagccat ccacaaggcc gtcgatcgcc taaaccatgc
     1861 caccgagacc tttgccgcac gccgcatgga tgcctcggtg aaacaggccc ttgccggcca
     1921 aaacctcaat acgctggaac tctagacatg cctcaaatca tcgttcttcc ccatcaggaa
     1981 ctctgcccag agggtgcagc catcgatgcc aaacccggcg tctcgatctg cgagaccctg
     2041 ctcgagcacg atatcgacat cgaacatgcc tgcgaccagg cctgtgcctg caccacctgc
     2101 cacgtcatcg tgcgtgaggg tttcgattcg ctgaacccgg ccgaagacaa ggaagaagac
     2161 ctgctggata gagcatgggg attggaaccc aaatcccgct tgtcctgcca ggccatcgtc
     2221 gccgaccggg acctggtggt cgagatcccc aagtactcga tcaacatggc caaggaaggc
     2281 atgcccaaac ccaaggcctg accctcctgc cggcaagcgg cagccatgac gcagcgcact
     2341 agcaataaaa aacccccgca tcactgcggg ggtttttgtt tggactcacc ggtgaggccg
     2401 cttgcgcgac cgtcacatta gaacttgtag gtgatgtcag cctggtacag gttgaagcta
     2461 tccaggttga gggcgatatc gccaccagtg ccaacggcag ccaggtcgtc gttcttgcga
     2521 ctaccgtggc cagcagccag gttgaggtag acgttgtcgc gcagcaggta ttcacccttg
     2581 aacacgacac ccttcatgtt gatgcgggaa tccatgaagt cggaatcgac agcgttcgga
     2641 tccagtgcat agacaccgac atcctggtac cagatcttgg cggaccagtc acccttcatc
     2701 gcggtcttac cttgcgactt gccgaacccg acgcccagca gccaggcctt gtcgtcctta
     2761 ccagcagcgc gaatcgcggc agcgttggcg ccaccggctg caacagcagc gtcgaagcgg
     2821 tcatcgccat cgatgttctt gacgtaatcg ctgaatgcgg tcagcttcat gccgtcattg
     2881 agcttgaacg ccagttccaa tggaacttcg atggtggtga tgtcgttggt agcgcctgga
     2941 ttggtaccca gggaggtacc gttgccgaga ccaggtacga atgtggaggc gccgggcgcg
     3001 tgatcattgg tgaacttggt gaaggtcaac gcggccttgg cggaaaccgt cttggtcagg
     3061 tcgaacttgg cgccgccctg gaaggccagc agattgttcg aagctgagtc actggtgtcg
     3121 atgctgaaat ccttgcggtc acccttgtac tgcgattgcg cgaagttacc gaacag
//
LOCUS       DATMTW010000038         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_473723, whole genome shotgun sequence.
ACCESSION   DATMTW010000038 DATMTW010000000
VERSION     DATMTW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473723"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VN084_00765"
     CDS             <1..422
                     /locus_tag="VN084_00765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWT28012.1"
                     /translation="YDRYLVRIEEMRQSNRIIKQCIDWLRKNPGPVITDNTKVAPPNR
                     EGMKQDMEALIHHFKLFTEGFHIPAGEAYAAVEHPKGVFGIYLISDGANKPYRLKIRA
                     PGFPHLAALDEMTRGHMIADLVAIIGTQDIVFGEIDR"
     gene            559..1032
                     /gene="nuoE"
                     /locus_tag="VN084_00770"
     CDS             559..1032
                     /gene="nuoE"
                     /locus_tag="VN084_00770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWT28013.1"
                     /translation="MLSAESIAKIEYELTKYPAENRQAAVMSALRIAQTEKGWLSKET
                     IAWVADYLGIPAIAALEVATFYNMYELEPIGKYKITVCTNISCMLRDSDTIVEHLNQK
                     LGIGFNETTPDGKFTLKEGECMGTCGGAPLFHINNTRMCEFLTPEKVDAILEELE"
     gene            1032..>2314
                     /gene="nuoF"
                     /locus_tag="VN084_00775"
     CDS             1032..>2314
                     /gene="nuoF"
                     /locus_tag="VN084_00775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWT28014.1"
                     /translation="MATYKPVIAHNLDKQTLVTLRTIKEAQPHTLETYVKLGGYAQLK
                     RIVTEKMKATDIITQVKLSNLRGRGGAGFPTGLKWSFMPRYFDGTKYLVCNTDEGEPG
                     TFKDRDIIRYNPHQLIEGMAIAAYTLGARVGYNYIHGEIWEDYEIFEEALAEARTAGF
                     LGENLFGTNFSFDLYAVHGYGAYICGEETALIESIEGKKGQPRFKPPFPASFGLYGKP
                     TTINNTETFAAVPWIIRNGGQAYLECGKPNNGGTKIYSVSGDVELPGNYEIPLGTPFS
                     KLLELCGGVKGGKKLKAVIPGGSSAPVLPAHIMMECTMDYDSIAKAGSMLGSGAVIVM
                     DDSRSMVESLRRLSYFYMHESCGQCTPCREGTGWLYRVVDRIHNGQGRASDMDLLNSV
                     ADNIQGRTICALGDAAAMPVRAMIKHFRHEFEALVP"
BASE COUNT          498 a          719 c          681 g          416 t
ORIGIN      
        1 gctatgaccg ctacctggtg cgcatcgaag agatgcgtca atccaaccgc atcatcaagc
       61 aatgtatcga ctggctgcgc aagaacccgg gcccggtcat caccgacaac accaaggtcg
      121 cgccgccgaa tcgcgaaggc atgaagcagg acatggaggc gctgatccat cacttcaagc
      181 tgttcaccga gggctttcat atcccggccg gcgaagcgta tgcggcggtc gagcatccga
      241 agggcgtgtt cggcatctac ctgatctctg acggggccaa caagccttac cgtctcaaga
      301 tccgtgcgcc gggtttcccg catctggcgg cgctggatga gatgacgcgt ggccacatga
      361 tcgctgatct ggtcgcgatc atcggcacgc aagacattgt attcggcgag atagaccgat
      421 gagtatcgtc tttcacaccg atactttgat gctcgctagc gcgcgatcct cacatgctgc
      481 tctgtacgct ccggttgctt gcaagcttgc gccgcgtctc ggcgcgaaat actcgctcat
      541 cggaagtgga atctagttat gttgtccgca gaatcaattg ccaagatcga atacgaactg
      601 accaaatacc cggcggagaa ccgccaggcc gcagtcatgt cggcgttgcg catcgcgcag
      661 accgaaaagg gctggttgtc caaggagacc atcgcctggg tcgccgatta cctgggcatc
      721 ccggccatcg ccgcgcttga ggtcgccacc ttctacaata tgtacgagct cgaacccatc
      781 ggcaaataca agatcacggt ttgcaccaat atctcctgca tgctgcgaga ttcggacact
      841 atcgtcgagc acctgaacca gaagctcggt atcggcttca acgagaccac cccggacggc
      901 aagttcacgc tgaaagaggg cgagtgcatg ggcacctgcg gcggcgcgcc gttgttccat
      961 atcaacaaca cgcgcatgtg cgagttcctg accccggaaa aggtcgatgc gatcctggag
     1021 gagctggaat aatggcgacc tacaaacctg tgatcgcgca caacctcgac aagcaaacgc
     1081 tggtgacgct gcgcaccatc aaagaggcac agccgcatac gctggaaacc tacgtcaagc
     1141 tggggggcta tgcgcaattg aaacgcatcg tcaccgagaa gatgaaagcc accgacatca
     1201 tcacccaggt caaattgtcg aatctgcgtg gccggggcgg cgcaggcttt cccaccggtc
     1261 tcaagtggag cttcatgccg cgttatttcg atggcaccaa atacctggtc tgcaacaccg
     1321 atgaaggcga accgggcacc ttcaaggatc gcgacatcat ccgttacaac ccgcaccagc
     1381 tcatcgaagg catggccatc gccgcctata cgctgggcgc gcgcgtcggc tacaactaca
     1441 tccatggcga gatctgggaa gactatgaga tcttcgagga ggcgctggct gaagctcgca
     1501 cagccggctt cctgggtgag aacctgttcg gcaccaattt cagtttcgac ctgtatgcgg
     1561 tgcatggtta tggcgcttac atctgcggcg aagagaccgc gctgatcgag tcgatcgaag
     1621 gcaagaaggg ccagccacgc ttcaagccgc ctttcccggc gagcttcggc ctctacggca
     1681 agccgaccac gatcaacaac accgagacct tcgcggcggt gccctggatc atccgcaacg
     1741 gcgggcaggc ctacctcgaa tgcggcaagc cgaacaacgg cggcaccaag atctactcgg
     1801 tgagcggcga cgtcgagctg cccggcaact acgagatccc gctcggcacg ccgttttcca
     1861 agctgctgga gctgtgtggc ggcgtcaagg gcggcaagaa gctcaaggcc gtgatcccgg
     1921 gtggatcttc cgcgccggtg ctgccggcgc acatcatgat ggagtgcacg atggactacg
     1981 actccatcgc caaggccggc tcgatgctcg gctccggcgc ggtgatcgtc atggacgaca
     2041 gccgctcgat ggtcgagtcg ctgcgccgcc tctcgtactt ctacatgcac gagtcctgcg
     2101 gccagtgcac gccgtgccgc gaaggcacgg gctggctcta ccgcgtggtg gaccgcatcc
     2161 acaacgggca gggcagggcg tcggacatgg acctgctgaa ctccgtggca gacaacatcc
     2221 agggccgcac catctgcgcg ctgggcgatg cggccgccat gcccgtgcgg gccatgatca
     2281 agcacttccg ccacgagttc gaggcgctgg tgcc
//
LOCUS       DATMTW010000039         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_641517, whole genome shotgun sequence.
ACCESSION   DATMTW010000039 DATMTW010000000
VERSION     DATMTW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641517"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="dusB"
                     /locus_tag="VN084_00780"
     CDS             complement(<1..860)
                     /gene="dusB"
                     /locus_tag="VN084_00780"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWT28015.1"
                     /translation="MQIGPYKLKNNLVVAPMAGVTDRPFRMLCKKMGAGLAVSEMVTS
                     NSLLYGSAKTLRRANHEGEVEPISVQIAGADPKMMAEAARYNVDNGAQIIDINMGCPA
                     KKICNVMAGSALLKDEPLVSQILKAVVGAVDVPVTLKIRTGWDKQNRNAVNVARMAED
                     IGVQALAMHGRTRACAYMGDAEYDTIAAVKQAIKIPLIANGDITTPEKAKFVLDYTQA
                     DAVMIGRAAQGRPWIFREIEHYLATGTHMLPPTVSEIHAVMLEHLHDLYAFYGDLTGM
                     RVARKHISWYT"
     gene            complement(1040..1303)
                     /locus_tag="VN084_00785"
     CDS             complement(1040..1303)
                     /locus_tag="VN084_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HWT28016.1"
                     /translation="MHCRSGCGACCIAPSINSPIPGMPNGKPAGVRCVQLLDDMRCAI
                     FGKPERPAFCGGIQPSEEMCGETREYAVHWLSTLEDQTKPHSV"
     gene            complement(1303..2091)
                     /locus_tag="VN084_00790"
     CDS             complement(1303..2091)
                     /locus_tag="VN084_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWT28017.1"
                     /translation="MTNQQATPGFGVIIIGDEILSGKRQDAHLTQAIATLKARGLSLG
                     WAQYLGDDPAQITATLKRTMASDDVVFCFGGIGATPDDHTRQSAAAAAGVPIERHAGA
                     VAEIEAQFGESAYPKRVLMADFPKGADLIPNPVNRVAGFSLGRHYFMPGFPQMAWPMM
                     AWVLDTHYAEHFHRQPQIEAAILVMDAGESQLIDLMNRIVVAYPDTKLFSLPKLDTRR
                     TIELGLKGPATHVEAGMREIRDAISAAGFAWTDLPSGQAPEQAQ"
     gene            2265..2513
                     /locus_tag="VN084_00795"
     CDS             2265..2513
                     /locus_tag="VN084_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWT28018.1"
                     /translation="MSHEQEHPDEEVMCLCSGTTCGQIRVMFRQGLDAEAISRKTGAL
                     TGCGGCEWDLAAFLAALAEQSAASDIPRDSRGRPLPAA"
     gene            complement(2510..5305)
                     /gene="polA"
                     /locus_tag="VN084_00800"
     CDS             complement(2510..5305)
                     /gene="polA"
                     /locus_tag="VN084_00800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWT28019.1"
                     /translation="MKTLLLVDGSSYLYRAFHAMPDLRNKQNEPTGAIQGVLNMLRRL
                     HKDYPSDYSACVFDAKGKTFRDDIYPEYKANRAAMPDDLRAQIEPLHEAIKAMGWPLL
                     AIEGVEADDVIGCLAKWAEREGMRVIISTGDKDISQLVNAHVTVVNTMRDAFRKTDEV
                     LDVAGVENKFGIPPSLIVDYLTLIGDTSDNVPGVEKVGPKTAVKWLKEYGNLDNIVAN
                     ADQISGVVGENLRKALDWLPTGRELITIRCALDHIDGIPNDFEHLRPQTPDKPKLAEL
                     FERFDFKTWRRELENMPGEAIPASAAPSSPAQTPSAASSHSPATQDLFAAPATRSRDY
                     EVILTETALDAWLNKIKQASLICFDTETTALDPMAARLVGMSFAVEAGQAAYLPLKHD
                     YAGAPQQLDFATTLAKIKPLLEDQAIKKVGQNLKYDKHVLANHGIALNGIAHDTLLQS
                     YVLESHRSHGMDDLAMRHLGVQTITFEEVAGKGAKQVGFNQVSVEQATDYAAEDADIT
                     LQLHQNLYPQIQPDPKLDYIYHQIEMPVMEILFTIERNGVLIDSQMLSRQSHELGQKL
                     LELEQQAHQLAGQPFNLGSPKQLQEILFGKLGIKPLKKTPSGAPSTDEDVLQELALDY
                     PLPKVILEHRSLAKLKSTYTDKLPKAVNADTGRVHTNYSQAVANTGRLSSNEPNLQNI
                     PVRTAEGRRIREAFIAPPGSVIVSADYSQIELRIMAHFSKDAGLLQAFAAGEDIHRAT
                     ASEIFGVEREAVSSEQRRYAKVINFGLIYGMSAFGLAQNLNIERSAAASYIERYFARY
                     PGVKDYMQDTRETAKQQGYVETLFGRRLWVPEINSPNGMRRAGAERAAINAPMQGTAA
                     DLIKLAMIAVQGWLEKESLQSKLIMQVHDELVLEVPETELEMVKTRLPQLMQGVAKLD
                     VPLLAEVGVGPNWEAAH"
     gene            5319..>5776
                     /locus_tag="VN084_00805"
     CDS             5319..>5776
                     /locus_tag="VN084_00805"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HWT28020.1"
                     /translation="MSAAKKIPKVIDPDMLQNNHTARESWHAFEIMAEFVDATERLKQ
                     ITPAVSIFGSARTAPDHPYYKLTAEIAHQLSDAGFSVISGGGPGIMEAANKGAYYGKS
                     LSVGLNIELPHEQVSNPFQDLSQNFSHFYMRKVMFVKNASAYVVMPGGFGT"
BASE COUNT          979 a         1708 c         1862 g         1227 t
ORIGIN      
        1 gtgtaccacg agatatgctt gcgcgcgacg cgcataccgg tcaggtcgcc gtagaaggcg
       61 taaaggtcat gcaggtgttc gagcatgacg gcatggatct cgctgacggt tggcggcagc
      121 atgtgcgtgc cagtggcgag gtagtgctcg atctcgcgga agatccacgg gcggccctgg
      181 gctgcgcggc cgatcatcac cgcatcagcc tgggtgtaat ccagaacgaa tttggctttt
      241 tccggcgtgg tgatatcgcc gttggcgatg agcgggatct tgatcgcctg cttgacggca
      301 gcgatggtgt cgtactcggc gtcgcccatg taagcacagg cgcgcgtgcg gccatgcatg
      361 gccagcgcct gcacgccgat atcctcggcc attctggcga cgttgacggc gttgcggttc
      421 tgcttgtccc agccggtgcg gatcttcagc gtcaccggca catcgacggc gccgaccacg
      481 gccttgagga tctgcgagac gagtggctcg tccttcaaca gggcagagcc ggccatgacg
      541 ttacagatct tcttcgccgg gcaacccatg ttgatatcga tgatctgggc gccgttatcc
      601 acgttgtacc tggccgcttc ggccatcatc ttcgggtcgg cgccggcgat ctgcaccgag
      661 atcggttcga cttcgccttc gtgattggcg cggcgtaacg tcttggcgct gccatagagc
      721 agggagttcg acgtcaccat ctcgctgacg gcgagcccgg cacccatctt cttgcacagc
      781 atgcggaaag gccgatcggt gacgcccgcc atgggggcga ccaccagatt gttcttcagt
      841 ttgtatgggc cgatctgcat cgcagcgtat tcgagtcttc gtaaaaagtg gggcgtcaat
      901 aaagtcggtc tgcctgcgcg gcttggaggc aaggcctcga cagcatgcgg agacgacgga
      961 aagttgccta ttttatatgc aaagtgctca tgcgcaaacc tccgattaca tccgccctgg
     1021 ctgccggtcg aaatgatcgc tagaccgaat gcggtttcgt ctggtcttcc agtgtggaca
     1081 gccagtgaac ggcatactcg cgcgtttcgc cgcacatctc ctcgcttggc tggatgccgc
     1141 cgcagaacgc cgggcgttcg ggtttaccga agatggcgca acgcatgtca tccagcagtt
     1201 gtacgcagcg cacgccggcc ggtttgccgt tgggcatgcc ggggatggga ctgttgatcg
     1261 aaggggcgat gcagcaggcg ccgcagcccg aacggcaatg catcactggg cctgctcggg
     1321 agcctgtcca gaaggcaggt cagtccatgc gaacccggcg gcggagatgg cgtcgcggat
     1381 ctcacgcatg ccggcctcga catgcgtggc cgggcccttg agaccaagct cgatggtgcg
     1441 gcgcgtatcg agtttgggca ggctgaacag tttggtatcc ggataggcga cgacgatacg
     1501 gttcatgagg tcgatgagct ggctttcgcc ggcatccatc accaggatgg ccgcttcgat
     1561 ctgcggctgc cggtggaagt gctcggcgta atgcgtgtcc agcacccatg ccatcatcgg
     1621 ccaggccatc tgcgggaagc ccggcatgaa gtaatgccgg cccagcgaga agccggcgac
     1681 gcggttgacc gggttgggga tgaggtcggc gcctttgggg aaatccgcca tcaatacgcg
     1741 tttggggtac gcgctctcgc cgaactgcgc ttcgatctcg gcgacggcgc cggcatggcg
     1801 ttcgatcggc acgccggctg cagcggcggc gctttgccgc gtatggtcgt ccggcgtggc
     1861 gccgataccg ccaaagcaga agactacgtc atcgctggcc atggtgcgct tgagcgtggc
     1921 ggtgatctgc gccgggtcgt cgcccaggta ttgcgcccag ccaaggctga ggccgcgcgc
     1981 cttgagcgtg gcgatggcct gcgtcaggtg cgcgtcctgt cgcttacccg agaggatctc
     2041 atcgccgatg atgatgacac cgaagcccgg tgtcgcctgc tggttcgtca ctttgcatgt
     2101 tccccattga atcaagaccc ggcttcgacc agccggatgt cgttgtgttg caagatcatg
     2161 gcgatttcac ggccaggcca tgtcggcatc gttgtttgcc ggggaaactt ccccgcttac
     2221 aataggctct actacatcgt tccaatcgac atccccagac catcatgagc cacgagcaag
     2281 aacatccgga cgaagaggtc atgtgcctct gcagcggcac cacctgcggc cagatccgtg
     2341 tcatgttccg gcaggggctg gatgccgaag ccatctcccg caagaccggc gcgctcacgg
     2401 gttgcggcgg ctgcgagtgg gatcttgcgg cgtttctcgc ggcgctggcc gagcaatccg
     2461 cggcttcgga catcccgcgc gactcgcgcg gtcgcccatt gcccgcagct taatgcgcgg
     2521 cttcccaatt cggaccgacg ccgacttccg ccagtagcgg tacatccagc ttggccacgc
     2581 cctgcatgag ctgcggcagc cgagtcttga ccatctccag ttcggtttcc ggcacttcca
     2641 gcaccagctc gtcatgcact tgcatgatga gcttgctctg cagactttcc ttttccagcc
     2701 agccttgcac cgcgatcatc gccagcttga tgagatcggc cgccgtaccc tgcatgggcg
     2761 cgttgatcgc cgcgcgctct gcaccagccc ggcgcatgcc gttggggctg ttgatctccg
     2821 gcacccagag gcgcctgccg aacagggtct cgacgtagcc ttgctgcttc gcggtctcgc
     2881 gcgtgtcctg catgtaatcc ttgacgccag gatagcgggc gaaatagcgc tcgatatagc
     2941 tggcggcggc gctgcgttcg atattgaggt tctgcgccag gccgaacgcg ctcatgccgt
     3001 agatgaggcc gaagttgatg accttggcat agcgacgctg ttcgctgctg acggcctcac
     3061 gttccacacc gaagatctcc gaggcggtgg cgcggtggat gtcctcgccg gcagcgaaag
     3121 cctgcagcag ccccgcatcc ttggaaaagt gagccatgat gcgcagctcg atctgcgagt
     3181 agtcggcgga gacgatgacg ctgcctggcg gcgcgatgaa ggcttcgcgg atgcgacggc
     3241 cctcggccgt gcgtaccggg atgttctgca ggttcggttc gttactcgac aggcggccgg
     3301 tgttggccac ggcctgactg tagttggtgt gcacacggcc ggtatctgcg ttgacggcct
     3361 tggggagctt gtcggtgtag gtggacttga gcttggccag gctgcgatgt tcgaggatga
     3421 ccttgggtag cgggtaatcc agtgccagct cctgcagcac atcctcgtcg gtcgagggcg
     3481 cgcctgaggg tgtcttcttc agtggcttga tgccgagctt gccgaacaat atctcctgta
     3541 gctgcttggg tgagccgagg ttgaacggct ggccggcgag ctggtgcgcc tgctgttcca
     3601 gttccagcag tttctggccg agctcatggc tctggcggct cagcatctgg ctatcgatga
     3661 gtacgccatt gcgttcgatt gtgaacagga tctccatcac cggcatctcg atctggtgat
     3721 agatatagtc gagtttcgga tcaggctgga tctgcgggta gaggttctgg tgcagctgca
     3781 gcgtgatgtc ggcgtcctct gcggcataat ccgtcgcctg ttcgaccgac acctgattga
     3841 agcccacctg tttcgcgccc ttgccggcga cctcctcgaa agtgatggtc tgcacgccga
     3901 gatggcgcat cgcgaggtca tccatgccgt gcgagcgatg gctctccagg acataggatt
     3961 gcagcagcgt atcgtgcgcg atgccgttga gcgcgatacc gtgattggcc agcacatgtt
     4021 tgtcgtactt gaggttctgg cctactttct tgatcgcttg gtcttccagc agcggcttga
     4081 tcttggccag cgtggtggcg aaatccagtt gctgcggcgc gccggcgtag tcgtgcttca
     4141 gcggcaggta agcggcctgg ccggcttcga cagcgaacga catgccgacg agccgcgcgg
     4201 ccatcgggtc gagcgccgtg gtctcggtat cgaagcagat caggctggcc tgcttgatct
     4261 tgttcagcca ggcgtcgagc gcggtttcgg tgaggatgac ttcgtaatcg cgggatcggg
     4321 tcgcgggtgc cgcgaacagg tcttgtgtgg caggtgaatg gctgctggcc gcgctgggcg
     4381 tttgcgctgg cgaggagggc gctgccgatg ccgggatggc ctcgcctggc atgttctcca
     4441 gttcgcggcg ccaggtcttg aaatcgaagc gctcgaacag ctcggccagc ttgggcttgt
     4501 ccggcgtctg tggccgcagg tgctcgaaat cattggggat gccgtcgatg tgatcgagcg
     4561 cgcagcggat ggtgatgagc tcgcgtccgg tcggcagcca gtccagcgct ttgcgcaggt
     4621 tctcgccgac cacgccgctg atctggtcag cgttggctac gatattgtcg agattgccgt
     4681 attccttgag ccatttgacc gcggtcttgg ggccgacctt ctccacgccc ggcacgttgt
     4741 ccgacgtgtc gccgatcagg gtcaggtagt cgacgatgag gctgggcggg atgccgaact
     4801 tgttctcgac gcctgccacg tcgagtacct cgtcggtctt gcgaaaggcg tcgcgcatgg
     4861 tgttaaccac ggtcacgtgc gcgttcacca gctgcgagat gtccttgtcg ccggtggaaa
     4921 tgatcacgcg catgccttcg cgctcggccc atttcgccag gcagccgatg acgtcatcgg
     4981 cctcgacgcc ttcgatcgcc agcaagggcc agcccatggc cttgatcgct tcatgcaaag
     5041 gttcgatctg cgcacgcagg tcgtcgggca tggcagcgcg gttggctttg tattcggggt
     5101 agatgtcgtc gcgaaaggtt ttgcccttgg catcgaagac gcaagcgcta taatcggacg
     5161 gatagtcttt atgcaggcgg cgcagcatgt tgagcacgcc ctggatcgca ccggtcggct
     5221 cgttctgctt gttgcgcaga tcgggcatgg cgtggaaggc gcgataaaga taggacgaac
     5281 cgtcgactaa caacaacgtt ttcatttgga tatccgctat gtctgctgct aaaaaaatcc
     5341 caaaagtgat cgatcccgac atgctgcaaa acaatcacac ggcgcgcgag tcgtggcatg
     5401 ccttcgagat tatggcagag ttcgtcgacg cgaccgagcg cctcaagcaa atcacgccgg
     5461 cggtctccat cttcggtagc gcgcgtactg cgcccgacca tccctattac aaactgaccg
     5521 cggagatcgc ccaccagctc tctgacgcag gcttcagcgt catctccggc ggcggccccg
     5581 gtatcatgga agccgccaac aagggcgcgt attacggcaa atcgctgagt gtcggcctca
     5641 acatcgaatt gccgcacgag caggtcagca atccgttcca ggatctcagc cagaatttct
     5701 cgcactttta tatgcgtaag gtcatgttcg tcaagaacgc ctcggcttat gtcgtcatgc
     5761 ccggtgggtt cggtac
//
LOCUS       DATMTW010000040         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_641572, whole genome shotgun sequence.
ACCESSION   DATMTW010000040 DATMTW010000000
VERSION     DATMTW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641572"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VN084_00810"
     CDS             <1..458
                     /locus_tag="VN084_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWT28021.1"
                     /translation="NARQRHPRGVPHKIGRDRPETRLTRPMVRILLAEDDDSMRTYLA
                     RALERSGYEVVAVETGAQAVPYIEADQFDLLLTDIVMPEMDGIELAQHAATVAPNMRI
                     MFITGFAAVTLKAGKAVPQAKVLSKPFHLRDLVLEIDRIFGTTSMTGLN"
     gene            complement(466..>1511)
                     /gene="dctA"
                     /locus_tag="VN084_00815"
     CDS             complement(466..>1511)
                     /gene="dctA"
                     /locus_tag="VN084_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HWT28022.1"
                     /translation="TLALIVGLIVANVVHPGAGLNIDPATLDAGKVAQYQQQAHDQSL
                     TGFLMDIIPATLISAVADGHNILQVLFVAILFGIAMTLIGEKAEPLMQVLESASHAIF
                     KLVSILMKAAPIGALGAMAFTIGKYGVGALANLVELVATFYLTSLLFVLVVLGLVARL
                     IGFNILHLIRYLKAELLLVLGTSSSEAALPSLIEKMERAGCRKSVVGLVVPTGYSFNL
                     DGTNIYMTLAALFIAQATNVDLSIGDQILLLLVAMLSSKGAAGVTGAGFITLAATLSI
                     VPSVPVAGMTLILGVDRFMSECRSLTNFIGNAVATVVVSAWERGLDRERFAAAMAGEA
                     LPPTPDMEEVVPG"
BASE COUNT          309 a          460 c          482 g          260 t
ORIGIN      
        1 ataatgcccg gcagcgccac ccgcgagggg tgccgcacaa gatcggccga gaccggccag
       61 agacgagact gacccgacca atggttcgaa ttctgctggc cgaagatgat gacagcatgc
      121 gtacctatct cgcccgcgcg cttgagcgtt cgggctatga ggtggtggcc gtcgagaccg
      181 gcgcgcaggc ggtgccctat atcgaagcgg atcagttcga cctgttgctg accgatatcg
      241 tcatgcccga gatggacggg atcgaacttg cccagcatgc ggccacggtc gcgccgaaca
      301 tgcgcatcat gttcatcacc ggctttgctg ccgtgacgct gaaggctggc aaggcggtgc
      361 cccaggccaa ggtgctgtcc aaacccttcc acctgcgcga cctggttctg gagatcgacc
      421 gcatcttcgg aacgaccagc atgaccggcc tcaactgaaa gccgcttagc cggggacgac
      481 ttcttccatg tcgggggtcg ggggcagtgc ttctcccgcc atggccgccg cgaatcgctc
      541 gcgatcaagg cccctctccc acgccgaaac gacgaccgtg gcgaccgcat tgccgatgaa
      601 gttcgtaagg ctgcggcatt cgctcatgaa ccgatcaacg cccaggatca gcgtcatgcc
      661 cgcgaccggc actgatggga cgatcgagag cgtagcggca agtgtgatga agcctgcgcc
      721 cgtgacgcca gccgcgccct tgctggacaa catcgcgacc agcagcagca ggatctgatc
      781 gcctatgctg agatcgacat tggtcgcttg ggcgatgaac agcgccgcca gggtcatgta
      841 gatgttcgtg ccgtcaaggt tgaagctgta gccggtcggc accaccagtc cgacgaccga
      901 ctttcggcag cctgctcgct ccatcttctc gatcaggctg ggcagcgcgg cctcggatga
      961 ggaggtgccg agtacgagca gcagttccgc cttcagatag cggatgaggt gcaggatgtt
     1021 aaagccaatc agcctcgcca caaggccaag cacgaccagc acgaacaaga gcgaggtgag
     1081 gtagaaggtc gcgaccaatt ccacgagatt tgccagcgcg cccacgccat atttgccgat
     1141 ggtgaaggcc atggcgccca gcgcgccgat gggggcagcc ttcatcagga tcgagacgag
     1201 cttgaaaatg gcgtggctgg cggattccag cacctgcatc aggggctccg ccttctcgcc
     1261 gatcagggtc atggcaatgc cgaacagaat ggcgacgaac aggacttgca gaatattgtg
     1321 gccgtccgcg accgccgaga tcagcgtggc cgggatgatg tccatcagga agccggtgag
     1381 tgactggtca tgcgcctgct gctgatattg cgcgactttg cccgcatcga gcgtcgccgg
     1441 gtcgatattg aggccagcgc cggggtgcac gacattggcg acgatcaagc cgacgatcag
     1501 cgcaagggta g
//
LOCUS       DATMTW010000041         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_339593, whole genome shotgun sequence.
ACCESSION   DATMTW010000041 DATMTW010000000
VERSION     DATMTW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339593"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..979
                     /locus_tag="VN084_00820"
     CDS             122..979
                     /locus_tag="VN084_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWT28023.1"
                     /translation="MLQALLRLFAILPLPVVHALGVLTGYISYITDRKFSRRIRNNLA
                     STGIATATSAYRKLVRRNAAETGKGILETFAVWFRSPAATLQWVKACHGWEHVEQALA
                     SGKGLIFLTPHLGCYEITALYYAARHPITVLYRPPRQRWLAPLIEQGRNRSRITLAAT
                     NMRGVRSLLKALKQGEAIGILPDQVPEFGEGVWADFFGKPAYTMSLVSKLAESTEATV
                     LMAFGERLSWGRGFVIHIAPLDLPPTPANINLGIEHLVRQRPAQYLWSYRRYKQPQQR
                     RNKPSDTTT"
     gene            1046..1879
                     /gene="dapF"
                     /locus_tag="VN084_00825"
     CDS             1046..1879
                     /gene="dapF"
                     /locus_tag="VN084_00825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWT28024.1"
                     /translation="MTLKFTKMQGIGNDFVVIDGYTAPVSLSAEQIRYIANRHFGVGC
                     DQLLMVERATQENIDFRYRIFNADGGEVEQCGNGARCFVRFVHEQGLTEKAEISVETA
                     SGIIYPRLEEDGNVTVNMGAPDFSPEHVPFIADAPALTYLLDVAGQAVDIGVVSMGNP
                     HAVQIVTNVEDAPVAQQGPIIEAHPRFPQRVNAGFMQVVSAHEIRLRVYERGSGETLA
                     CGTGACAAVVTGIRQGLLESPVKVNTHGGVLHISWAGGTSPVWMTGPAMTVFQGEIAL
                     Q"
     gene            1937..2608
                     /locus_tag="VN084_00830"
     CDS             1937..2608
                     /locus_tag="VN084_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HWT28025.1"
                     /translation="MDNDTPLSELDVGNYLKAHPQFFEHHAELLTEMYLPSPHGSGTV
                     SLAERQQLLQRDRIRVLEAKMADFLKFGEENDVTSEKVHRLSLGLLASPNLEVLVQIL
                     THTLREDFQVPHVGLRLWASPMNQLDREQPIFQPVDDELRNWADGLLTPYCGHRPGLE
                     MDSWFGENATPKSFALVALRSEKVFGLLAMAAEDEQRFYPEMGTLFLKRIGELVSAAL
                     LRYIE"
     gene            2613..3488
                     /gene="xerC"
                     /locus_tag="VN084_00835"
     CDS             2613..3488
                     /gene="xerC"
                     /locus_tag="VN084_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWT28026.1"
                     /translation="MELLAEYLAYLSFERGLSPLTREHYARDIRLLLDAAKDQALDSL
                     DASHIRRYIAALHGRGLSGKSIARCLSAWRGFFNFLIQRKGFDSNPCSGLKAPKSPRT
                     LPQALSTDQAVSLVAIEGDDCLTLRDRAILELFYSSGLRLAELIGLDINALDLDNGTV
                     TVTGKGSKTRIVPMGSHAILAIRKWLVARAAIPQVDAQALFVSQHGKRLTSRAIQYRI
                     KQWAIRQGINADVHPHMLRHSFATHVLQSSGDLRAVQEMLGHANISTTQVYTHLDFQH
                     LAKVYDAAHPRARKK"
     gene            3652..>3846
                     /locus_tag="VN084_00840"
     CDS             3652..>3846
                     /locus_tag="VN084_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="SodB; iron binding; present under aerobic and
                     anaerobic conditions; destroys free radicals; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HWT28027.1"
                     /translation="MEHQLPALPYPKNALAPHISEETLEFHYGKHHQACVTNLNNLIK
                     GTEFENTGLEDIIKKASGGIY"
BASE COUNT          827 a         1229 c         1064 g          726 t
ORIGIN      
        1 aagtaaatag atattcgctc attggttcgg tctgtaccca gggattaaag taaaagcagc
       61 gggtttaaaa aatcaaaaga ttgctagaat agcaaaatca tcctgtttca aaaagaacta
      121 catgctccag gcattgctcc gcctcttcgc catcctgccc cttccggtcg tccatgcgct
      181 gggcgtgctg accggttata tcagctacat cactgaccgc aaattctccc gacgcatccg
      241 caacaatctc gccagcacgg gcatcgccac cgccactagc gcctatcgca aactcgtgcg
      301 ccgtaatgcg gccgaaacgg gcaaaggcat cctagagacc ttcgcggtct ggttccgttc
      361 gcccgcagcg acactacaat gggtcaaggc ctgccatggc tgggagcatg tggaacaggc
      421 gctggccagc ggcaagggtc tgatcttcct gacgccacac ctgggctgct acgagatcac
      481 cgcgctctac tacgccgcgc gccacccgat caccgtactc tatcgtccgc cacggcagcg
      541 ctggctcgcc cccttgatcg aacaggggcg taaccgcagc cggatcacgc ttgccgcaac
      601 caatatgcgc ggcgtacgta gcctgctcaa ggcacttaag cagggtgagg cgatcggcat
      661 cctgccggac caggtgcctg agttcggcga aggtgtctgg gcggattttt tcggcaagcc
      721 ggcctatacc atgagcctcg tgagcaaact ggcagagagc accgaggcca cggtgctcat
      781 ggcgttcggc gagcgtctat cctggggccg cggcttcgtc atccatatcg cgccattgga
      841 cttgccgccc acaccggcca acatcaatct cggcatcgag catctggtca ggcagcgccc
      901 ggcccaatac ctgtggagct accgccgcta caaacagccg cagcagcgac gcaacaagcc
      961 atcggacacg accacgtgag tgccgctatc ggcgccttgc acattgccct acaataaccg
     1021 tcacgcaacc cacccgctga tgcacatgac gctcaaattc accaagatgc aggggatcgg
     1081 caacgatttc gtcgtcatcg atggctacac tgccccggta tcgctctccg cagaacagat
     1141 ccgctacatc gccaatcgcc atttcggcgt tggctgcgac cagttgctga tggtcgagcg
     1201 cgcgacacag gaaaatatcg acttcagata ccgtatcttc aatgccgatg gtggcgaagt
     1261 cgaacagtgc ggcaacggtg cgcgctgctt cgtgcgcttc gtgcatgagc aggggctgac
     1321 ggaaaaggcc gagatcagcg tcgagactgc cagcggcatc atctacccca gattggaaga
     1381 ggacggcaat gtcacagtca acatgggtgc gccggacttt agccctgaac atgtgccgtt
     1441 catcgccgat gcgccagctc tgacctactt gctggacgtg gccggacagg ccgtcgacat
     1501 cggcgtcgtc tcgatgggca acccacacgc cgtgcagatc gtcaccaacg tcgaagacgc
     1561 gccggtcgcc cagcaaggac ctatcatcga agcgcatccg cgctttccac agcgcgtcaa
     1621 tgccgggttc atgcaggtcg tctctgcgca tgagatccgc ctgcgcgtct atgagcgcgg
     1681 cagcggcgag accctggcat gtggcacggg cgcttgcgcg gctgtggtca caggtatccg
     1741 ccagggattg ctggagagcc cagtgaaggt caacacccac ggcggcgttt tacacatcag
     1801 ctgggcaggc ggcaccagcc ctgtctggat gaccggcccc gcaatgacgg tgttccaggg
     1861 cgagatcgcg cttcaatgac cgcatgcttg cccggatccc cattcgccct atcgtcacgc
     1921 agatcaagga aaccccatgg ataacgacac cccgctgagc gaactcgacg tcggcaatta
     1981 cctcaaggcc cacccgcaat tcttcgaaca tcatgccgag ctgctgacgg agatgtactt
     2041 gcccagcccg catggcagcg ggacggtctc gctggccgaa cggcagcagc tgttgcagcg
     2101 ggatcgcatc cgggtactgg aagcgaagat ggccgacttc ctcaagttcg gtgaagagaa
     2161 cgacgtcacc agcgaaaaag tccaccggct gagcctcggc ctgctggcga gccccaattt
     2221 ggaagtgctg gtgcagattc tgacccatac cttgcgcgag gattttcagg tcccgcatgt
     2281 cgggctacgg ctgtgggcat cgccgatgaa ccagctcgac cgtgagcaac cgatcttcca
     2341 gccggtggac gatgaattgc gcaactgggc ggacggcctg ctcacaccgt attgcggcca
     2401 tcgtccaggg ctcgagatgg acagctggtt cggcgagaac gccactccca aatccttcgc
     2461 gctcgtcgcg ttacgtagcg aaaaggtgtt cggcctgttg gccatggcag ccgaagacga
     2521 gcagcgtttc tatcccgaga tgggtacgct gttcctcaag cgcatcggcg aactggtcag
     2581 cgctgccctc ttgcgctata tcgaatagtg tcttggaact gctcgccgaa tatctggcat
     2641 acctcagctt cgagcgcggc cttagtccgc tcacgcgcga gcattacgcc cgggacatcc
     2701 ggctactgct cgatgccgcc aaggatcaag cgctcgatag cctcgatgcc tcgcatatcc
     2761 ggcgctacat cgctgccctg catggacggg ggctgagcgg taaaagcatc gcccgctgcc
     2821 tgtcggcgtg gcgcggtttc ttcaacttcc tcatccagcg caagggcttc gacagcaacc
     2881 cctgcagcgg actcaaggcg ccgaaatccc ccaggacctt gccccaggcg ctgtccaccg
     2941 accaggcagt gagccttgtc gccatcgaag gcgatgactg cctgacgtta cgggaccgcg
     3001 ccatcctgga actcttctac tcctcgggtt tgcgcctggc agaactgatc ggtctcgaca
     3061 tcaatgccct cgacctcgat aacggcacgg tgacagtcac cggcaagggc agcaagacac
     3121 gtatcgtgcc catgggcagc catgcgatcc tcgccatccg caaatggctg gtcgcgcgcg
     3181 ccgccattcc gcaagtggat gcgcaagcgc ttttcgtcag ccagcatggc aaacgtctca
     3241 cgtcgcgcgc catccagtat cgcatcaagc agtgggcgat ccgccaaggc atcaatgccg
     3301 acgtccatcc gcatatgctt cgccatagct ttgccacgca tgtcttgcaa tcgagcggcg
     3361 atctgcgtgc agtgcaggag atgctggggc acgccaacat cagcacgacg caagtctaca
     3421 cacatctcga tttccagcat ctggccaagg tctacgacgc tgcccatccc cgcgcccgca
     3481 agaaatgatc gtagttgcta gcggcatcgg gacgtgacct gacacctgct tacctcgaag
     3541 gcgccacaat tgagaatggt tctcgataag taagatcggc gcgaaattat ggcaagattt
     3601 aggcttcgac ccctcagtgt ctatacccct tctagtttta aggattaaat catggaacat
     3661 caattacctg ctttacccta tccgaagaac gcactcgcac cgcacatttc cgaagagacc
     3721 ctggagttcc actatggcaa acaccaccag gcgtgcgtca ccaacctgaa caacctgatc
     3781 aagggcacgg aattcgagaa caccggtctc gaagacatca tcaagaaggc ttccggcggc
     3841 atctac
//
LOCUS       DATMTW010000042         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_406658, whole genome shotgun sequence.
ACCESSION   DATMTW010000042 DATMTW010000000
VERSION     DATMTW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406658"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1315)
                     /gene="lplT"
                     /locus_tag="VN084_00845"
     CDS             complement(98..1315)
                     /gene="lplT"
                     /locus_tag="VN084_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HWT28028.1"
                     /translation="MKISHSLFSRGMVAVLTAQFVSALADNALLFAAIALLKSQAAPG
                     WQIPMLQEFFVIAFILLAPFVGPFADTLPKGRVMLIANSLKLIGACAMLVGLHPLLAY
                     GMVGLGAAAYSPAKYGILSELVGPEKLVKANSMMEGSTIVAILLGAIVGGWLAAHSVN
                     TALASIATCYLLAMVANLLIPRLPPAHPLTHFSVPVLFSDFWHALRSLLGNRDARFTL
                     LGTSVFWGAGSTLRFLLVAWVPAALLIRDDAAALSMAANLSGVVAIGIALGAAAAARF
                     VSLKTVNRALPAGILIGGLIMAFAFVDHLYLAILLLILTGACGGFYVVPLNALLQERG
                     HETVGAGHAIAIQNFFENGCMLLMIGFYTLLDKQGVSVVDSTMIVGAVVLLAIGSLSL
                     LRYSQTRKMPRAP"
     gene            complement(1325..3394)
                     /gene="aas"
                     /locus_tag="VN084_00850"
     CDS             complement(1325..3394)
                     /gene="aas"
                     /locus_tag="VN084_00850"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HWT28029.1"
                     /translation="MLGKSNVPDQRGLLVIANHESFLDGLLLGLFLPVRATFVVHTTV
                     LNSWLFRQILRLTPYLAVDPTSPLAMKKVIKLLESGENVVIFPEGRITQTGSLMKVYD
                     GPGFVAAKTGAAILPVRLDGAARSYFSRLSGDYPHKLFPKVTLSILPATGIAMPQAAT
                     AKQRRRMAGEAMRRTMQEMLFQSQPVQTLFSAFLDAISVHGRKTRLVEDMRQVEESYG
                     ELLKKSLALGRLVEKISAPGEAVGLLMPNVSNTVCLIFGMSAMQRVPAMLNYTAGTAG
                     LQNACTAAKVKTIITSRQFVEAAKLADTVAALQDVELVYLEDLRSRFSLRDKLWLMGY
                     ALWLPRIATRNVTPDQAAVILFTSGSEGKPKGVVHSHRGILANIAQIRAVIDFSPTDK
                     FMIALPLFHAFGFTCGGIMPLVTGSKLFLYPSPLHYRVIPEVIYDRGCTVLFGTSTFL
                     GNYAKFAHPYDFYKLRYVVAGAEKLNDEVRKAWMDKFGIRILEGYGSTECAPVLAVNT
                     PMANLVGSVGPLLPGLQPRLESVPGIEHGGRLSVKGPNVMLGYYLYDAPGVLQRPADG
                     WYDTGDIVEIDMHGFVHIRGRVKRFAKVAGEMVSLETVEQIANAASPNTQQAATTQVD
                     AQRGESIVLFTTHASLTRESLQAAARTLGAPELAVPRKIVKVKELPLLGTGKIDYVQL
                     KQLAENA"
BASE COUNT          714 a          980 c         1055 g          645 t
ORIGIN      
        1 gggctcggac gttgactgaa atccatgtag gccgtggctg acacgtacca gtcctatcgg
       61 ggacaggtcg atagcgggca ctcactggac atcccactca cggcgcacgc ggcatcttcc
      121 gggtctggct ataacgtagc agcgacaggc taccgattgc cagcaatact acagcgccta
      181 cgatcatggt cgaatccact accgagacgc cctgcttgtc gagcaaggta tagaacccga
      241 tcatgagcag catgcagcca ttctcgaaaa agttctggat ggcgatggca tgaccggcgc
      301 cgacggtttc atgaccgcgc tcctgaagca aggcgttcaa tggcaccaca tagaagcctc
      361 cgcaagcgcc agtcaatatg agcaacagga tcgccaggta gaggtgatcg acgaatgcga
      421 acgccatgat caggccgccg atgaggatac cggccggcag ggcacgattg acggtcttga
      481 ggctgacgaa gcgggccgct gcggctgcgc ctagcgcgat gccgatggcg acaactccgc
      541 tcaggttggc cgccatgctc agtgccgcgg catcgtccct gatcagaagt gccgctggca
      601 cccaggccac cagcaggaag cgcagtgtgc tgccggcacc ccagaacacg ctggtaccga
      661 gtagcgtgaa acgagcatct ctattaccca gcaggctgcg cagggcgtgc caaaagtcac
      721 tgaacagcac gggcacggag aagtgcgtga gcggatgtgc tggtggcaag cgcgggatca
      781 gcaaattggc gaccatcgcc agcaggtagc acgtcgcgat gctggccagc gccgtattga
      841 ccgagtgcgc agccagccag ccgccgacga tcgcgccgag caggatggcg acgatggtcg
      901 agccttccat catgctgttg gccttgacca gcttttctgg cccgaccagt tcgctcagga
      961 tgccgtattt tgctggcgaa tatgcggcgg ctcccagccc caccatcccg taggcaagca
     1021 gggggtgaag accgaccagc atggcgcatg cgccgatgag cttcaggctg ttggcgatga
     1081 gcatgacccg gcctttgggt agtgtgtcgg caaatggccc gacaaaaggt gccagcagga
     1141 tgaaggcgat gacgaagaac tcctgcagca tcgggatctg ccagccaggt gccgcctggg
     1201 atttgagcaa ggcgatggcg gcgaacagta aggcattatc ggcgagcgcc gagacgaact
     1261 gtgcggtcag tacagcgacc atgcctcttg agaacagcga atgcgatatc ttcatggatt
     1321 tcccttatgc gttttctgcc aactgcttca attggacgta atcgatcttg ccggtaccaa
     1381 gcagcggcaa ctccttgacc ttgacgatct tgcgcggcac cgcgagctct ggcgcgccca
     1441 gcgttctggc ggcagcctgc aaggattctc gtgtcaaaga cgcatgcgtc gtgaagagta
     1501 cgatgctctc gccacgctga gcgtcgactt gcgtggtcgc ggcttgctgc gtatttgggg
     1561 atgcggcgtt ggcgatctgt tccacggttt ccagcgagac catctcgcct gcgaccttgg
     1621 cgaagcgttt gacacgcccc cggatatgca caaaaccatg catatcgatc tccacgatat
     1681 cgccggtgtc gtaccagccg tcggctgggc gttgcagtac gcccggcgcg tcatacaggt
     1741 aatagccgag catcacgttc ggacccttca ctgacaaccg gccgccgtgc tcgatgccag
     1801 gcacggattc cagcctgggt tgaagcccag gtaacagcgg accgacgctg ccgaccagat
     1861 tcgccatcgg cgtattgact gccagtacag gcgcacattc ggtgctgccg tacccttcca
     1921 ggatgcgaat gccaaacttg tccatccagg ccttgcgtac ctcgtcattg agcttttcgg
     1981 cgccagcgac cacatagcgt agcttgtaga aatcgtaggg gtgagcgaac ttggcgtaat
     2041 tgccgaggaa ggtgctggtg ccgaacagca ctgtgcagcc ccgatcgtag atgacctcgg
     2101 ggatgacgcg gtaatgcagg ggcgaaggat ataggaacaa tttgctgccg gtgaccagcg
     2161 gcatgatgcc gccgcaagtg aagccaaagg catggaacag cggcagcgcg atcatgaact
     2221 tgtcggttgg cgagaaatcg atgaccgcac ggatctgcgc gatattcgca aggatgccgc
     2281 gatgcgaatg caccacgccc ttgggtttgc cttcagaacc tgaggtgaag aggatgacgg
     2341 ccgcctgatc cggcgtcacg ttgcgtgtcg ctatgcgagg caaccacaag gcatagccca
     2401 tgagccagag tttgtcacgg aggctgaatc ggcttcgcag gtcttccaga tagacgagtt
     2461 cgacatcttg caaggcagcc accgtatcgg cgagtttcgc tgcctcgacg aattggcgtg
     2521 aagtgatgat ggtcttaacc ttggcggcgg tacatgcgtt ctgcaggccg gcggtgcctg
     2581 ccgtgtaatt cagcatcgcc ggcacacgct gcatggcgct catgccgaag atcaggcaga
     2641 cggtattcga cacattcggc atgagtagac cgacggcttc gcctggcgca ctgatctttt
     2701 ccactagccg gccgagtgcc aggctctttt tcagcagctc gccgtagctc tcttcgactt
     2761 gccgcatatc ttccaccagc cgggttttgc ggccatggac actgattgca tcaaggaacg
     2821 cgctgaacag cgtttgcacc ggctgtgact ggaacagcat ctcttgcatc gtgcggcgca
     2881 tcgcttcgcc tgccatgcgc cgccgctgtt tggcggtggc ggcctgcggc atggcgatgc
     2941 cggtggcggg caggatggac aaggtcacct tggggaaaag cttgtgcgga taatcgcctg
     3001 acaagcgact gaagtaagag cgagcagcac catcaagccg taccggcagg atcgcggcgc
     3061 cggtcttggc ggcgacgaag cccggaccgt catagacttt catgaggcta ccggtttggg
     3121 tgatgcgacc ttccggaaag atcacgacgt tctcgccgga ttccagtagc ttgatgactt
     3181 tcttcatcgc cagcgggctg gtcgggtcga ccgccagata aggcgtcaga cgcaggatct
     3241 ggcggaacag ccagctattg aggaccgtgg tatgcaccac gaacgtggca cgcaccggca
     3301 ggaataggcc aagcagcagg ccatcgagaa aggattcgtg attggcgatg accagcagac
     3361 cgcgctggtc aggcacattg gattttccga gcac
//
LOCUS       DATMTW010000043         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_440041, whole genome shotgun sequence.
ACCESSION   DATMTW010000043 DATMTW010000000
VERSION     DATMTW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440041"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VN084_00855"
     CDS             complement(<1..1339)
                     /locus_tag="VN084_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWT28030.1"
                     /translation="MAAQASAQPHLHKYRHGLRLLLRFLLYAVSFFFVCFGWWTSDNF
                     GEPPLDQVLYHLQFGMDGLVDTDAQLIRTFVLTAILLPTAVAFCLVATESAIGAYLTH
                     TARHHIYPAASIVNRSAKRVLKLIYWLISHRAPIYVFTASALYFGAQFSVLAFLHHQF
                     GKDYFAEHYVYPDQVNITPLQPRNLVLIYVESLENTYRQPALFQRNLLAGLDQLGGLS
                     FAAFRQAPGTGWTIAGMTASQCAIPLKSVSLYDGNAQGQNIKTFLPSAVCLGDILHRY
                     GYRNVYMGGDGKDFSGKGMFFQDHHYDEVYGRDELRSGEAHEELNEWGLFDDALLARA
                     RGKLDQLHQAGQPFNLTLSTIDTHGPWGHLSQLCKHRGARVFEDVVQCSANQVAEFVQ
                     FMRKRGYLKDTNVVIMGDHLAMWNPVYEDKLEPTAMRYVYNRLISIQPHAKNRD"
     gene            complement(1428..1928)
                     /locus_tag="VN084_00860"
     CDS             complement(1428..1928)
                     /locus_tag="VN084_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWT28031.1"
                     /translation="MKQTFLAGVMTLLLPAAASAAEPMALDACVKASLAMHPGKIKSL
                     SAEIEDGKRQYELDIKGLDGNRWEVECDATTGKINRVEREIHEHSDEFHSKARISREA
                     ATQAALAQYPGKVINTEYDIEDDGEISYEFIIERSDGRFTEIEVDAVTGKLAGEEEVL
                     YHIGGK"
BASE COUNT          409 a          612 c          614 g          431 t
ORIGIN      
        1 catcccggtt cttggcatgc ggctggatcg agatcaggcg attgtagaca taacgcatag
       61 cggtcggctc aagcttgtcc tcgtagaccg ggttccacat cgcaagatgg tcgcccatga
      121 tcaccacgtt ggtatccttg aggtagccgc gcttgcgcat gaactgtacg aactcggcga
      181 cctggttggc cgagcactgc accacatcct cgaacacgcg cgcgccgcga tgcttgcata
      241 gctgtgacag atgcccccat gggccgtggg tatcgatggt ggaaagcgtg aggttgaacg
      301 gctgcccggc ctgatgcaac tggtcgagct tgcctctggc tcgcgccagg agggcatcgt
      361 cgaacaagcc ccattcgttc agctcttcat gcgcctcacc gctgcgtagt tcatcgcgcc
      421 cgtagacttc gtcgtaatga tggtcctgga agaacatgcc cttgcccgaa aagtccttgc
      481 cgtcgccgcc catatagacg ttgcgatacc cgtagcgatg caggatatcg ccgaggcaga
      541 cagcgctggg caggaaagtc ttgatattct ggccttgcgc attgccgtca tagaggctga
      601 cgctcttcag cgggatcgcg cattgcgagg cggtcatgcc ggcgatggtc cagccggtcc
      661 ccggcgcctg gcggaatgcg gcgaaactga gcccgccgag ctggtcgagc cccgccagca
      721 ggttgcgctg gaacaaggcg ggctgccggt aggtgttctc caggctttcg acatagatga
      781 gcaccaggtt acgcggctgc agcggcgtga tgttgacctg atcgggatag acgtaatgct
      841 cggcgaaata atccttgccg aactgatgat gcagaaacgc cagcacggag aactgcgcgc
      901 caaaatacag cgcgctggcc gtgaacacat agatcggcgc gcgatggctg atcagccagt
      961 agatgagctt gaggacgcgt ttggctgagc ggttgacgat cgaagcggcc ggatagatgt
     1021 gatgtcgggc ggtgtgcgtg aggtaggcgc cgatcgcgga ttcggtcgcc accagacaaa
     1081 acgccaccgc cgtcggcagc agtatggcgg tcagtacaaa ggtgcggatc agttgggcat
     1141 cggtgtcgac caggccatcc atgccgaact gcaggtggta tagcacctgg tcgagcggcg
     1201 gctcgccgaa gttgtcgctg gtccaccagc cgaagcagac gaagaagaac gacaccgcat
     1261 acagcaggaa acgcagcagc agtcgcaagc catgccggta cttgtgcaga tggggttgcg
     1321 cagacgcctg tgctgccatg cctcgaatcc ctgccgccga ttaaaaggcg ttaagttaca
     1381 ccaatatgac aacagtgtct ggacgccacg catcatggcg aacgcgatca cttgccgccg
     1441 atgtggtaga gcacctcttc ttcgcccgcc agcttgccgg taacggcatc gacctcgatc
     1501 tcggtgaacc tgccgtcgct acgctcgatg atgaattcat aggagatctc gccgtcgtcc
     1561 tcgatatcgt attcggtgtt gatcaccttg cccggatatt gggccagcgc cgcctgtgtc
     1621 gccgcttcgc ggctgatcct ggctttgctg tggaattcat cgctatgttc gtggatctcg
     1681 cgctcgaccc gattgatctt gccggtcgtg gcgtcgcatt ccacttccca gcgattgccg
     1741 tcgagtccct tgatatcgag ttcatactgg cgcttgccgt cttcgatctc ggcgctcagc
     1801 gatttgatct tgcccggatg catcgccagc gacgctttca cgcacgcgtc aagcgccatg
     1861 ggttctgcgg ccgatgctgc ggccggtagc aacaaggtca tcacacctgc caaaaatgtc
     1921 tgcttcatgg tttcacctcc cgatcaaaaa tcccattaaa gaagattcgc actacacctg
     1981 ctttaacctg cgtcaaatcg ccgtgcattc gccacagggg cattgcccga tctcatcatg
     2041 tcatgctgcc ggatccgctg accgac
//
LOCUS       DATMTW010000044         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_37807, whole genome shotgun sequence.
ACCESSION   DATMTW010000044 DATMTW010000000
VERSION     DATMTW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37807"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..414)
                     /locus_tag="VN084_00865"
     CDS             complement(16..414)
                     /locus_tag="VN084_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWT28032.1"
                     /translation="MSVQLTNFLIHWAVMSLALWVASHIFKGISFSNRGALLVSALVL
                     GFLNAILRPILFFLTFPLTLLTFGLFLLVINALIIMITAKLVNGFKLSGFWTAFFASI
                     FIAIFGFFLEWVLPGNTHLIVVPSMHTVNI"
     gene            complement(429..1763)
                     /gene="hpnE"
                     /locus_tag="VN084_00870"
     CDS             complement(429..1763)
                     /gene="hpnE"
                     /locus_tag="VN084_00870"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HWT28033.1"
                     /translation="MAARLNPAGSNHVAVIGGGCAGLAAAARLASQGVRVTLFEASPH
                     FGGRARRVRWQGEALDNGQHILLGAYRDTLALLRLVGVDCEQALMRLPLQLEIQGHFK
                     LRACPWLPAPLHILVGLLTASGLRWDERWQAIRFMVWLKSIDFQLPQDLPLADLLARR
                     RQPEPVIRWLWEPLCLAALNTPLDTASAQVFLNVLRDSFARGKTDSDMLLPLQDLSAL
                     LAEPVIAYLRQHGGQCKPGCAVEAVEADDDGYRLVTTDGIADFSEVIIAVHPARATDL
                     LARLPSQAATRATTAALQYQPIYTIYIQYATDTRLPAPMLGLTDGIGQWVFDRGQLYG
                     QHGLMAVVISAEGAHQRLTQTRLAETIIAELSRQFPLLPAARWHKVIAEKRATFACTA
                     GLKRPAAITADKGLLLAGDYVAGDYPATIEGAVRSGFYCADHILARLTQPTS"
     gene            complement(1748..>2288)
                     /locus_tag="VN084_00875"
     CDS             complement(1748..>2288)
                     /locus_tag="VN084_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWT28034.1"
                     /translation="IIDGMEMDLDQNRYADFKQLQLYCYRVASVVGILSVAIFGYTNR
                     NTLKYAHDLGLAFQLTNIIRDVGEDARRDRIYLPLDELAQFGVTERDILQGRESDRVQ
                     QLLDFQIARASDFYDRALNALPIEDRKAQRTGLIMTAIYRTLLEEIKADGAQQVLNSR
                     TSLTPLRKLWLAWLTWLRA"
BASE COUNT          474 a          636 c          753 g          425 t
ORIGIN      
        1 catccgtgag ccgtatcaga tattcacggt atgcatcgac ggcactacga tcagatgcgt
       61 gttgcccggc aatacccact ccaggaagaa gccgaagata gcgatgaaga tactggcgaa
      121 gaatgctgtc cagaaaccgg agagcttgaa gccgttgacc agtttggcgg tgatcatgat
      181 gatcaaggcg ttgatcacca gcagaaagag gccaaaggtc agcagtgtca gcggaaaagt
      241 caggaagaac aggatcggac gcaggatcgc attgagaaag ccaagtacca gcgctgagac
      301 cagcaaggcg ccgcggttac tgaacgagat acctttgaag atatggctcg ctacccacag
      361 ggcaagactc atgacggccc aatggatcag gaaattggtg agttgtacgc tcatggttga
      421 tgtcctcgct aggatgttgg ttgggtgaga cgggcaagaa tgtggtcggc acagtagaag
      481 ccgctgcgaa cagcaccttc gatggtcgcg ggatagtcac cggcgacata gtcaccggcc
      541 agcaggaggc ctttgtcggc cgtgatcgcg gcgggccgtt tcagccccgc ggtgcaggcg
      601 aaggtggcgc gtttctccgc gatcacctta tgccaccgcg cggctggcaa tagtgggaat
      661 tggcgagaca gctcggcgat gatcgtctcg gcaagcctgg tttgggtaag tcgctgatgc
      721 gcaccctcgg cgctgatgac gaccgccatc aggccatgct ggccgtaaag ctggccgcga
      781 tcgaataccc attggccgat gccatcggtc aaacccagca tgggggctgg tagccgggta
      841 tcggtagcgt actggatata tatggtgtag atcggctgat actgcaacgc tgccgtggtg
      901 gcgcgggtgg ccgcctggct tggtagcctc gccaggagat ccgttgcgcg ggctggatgg
      961 acggcgatga tgacctcgct gaagtcggcg atgccgtccg tggtgacaag acgatagccg
     1021 tcgtcatcgg cttcgaccgc ttccaccgca cagcctggct tgcattgtcc gccatgttgc
     1081 cgcagatagg cgatgacagg ctctgcaagc aaggctgaga ggtcctgcag cggcaacagc
     1141 atatcgctgt cggtcttgcc gcgagcgaaa ctatcgcgta acacatttag gaacacttgc
     1201 gcgctggccg tgtcgagggg agtgttcagc gccgccagac atagcggttc ccatagccag
     1261 cggatgactg gttccggctg gcgccggcgg gccagcagat cggccagcgg tagatcctgc
     1321 ggcagctgaa aatcgatgct cttaagccag accatgaaac ggatcgcctg ccagcgctcg
     1381 tcccagcgca agccactggc ggtaagtaac ccgaccagga tatgcagtgg cgcgggcagc
     1441 caggggcagg cccggagctt gaagtgtccc tggatctcaa gttgcagcgg tagccgcatc
     1501 agggcttgtt cgcaatcgac gccgaccagt cgcaagagcg ccagggtgtc tcgatacgcg
     1561 ccgagcagga tgtgctgacc gttgtcgagc gcttcaccct gccagcgtac gcgccgtgcg
     1621 cggccaccaa aatgcgggct ggcttcgaac aaggtcacac gcacgccttg cgatgctagt
     1681 ctcgccgcgg ccgcgagacc ggcacagccg ccgccgatga cggcgacatg gttgctgccg
     1741 gcgggattca ggcgcgcagc catgtcagcc atgccagcca gagtttgcgc agcggcgtca
     1801 gcgaagtgcg cgaattcagc acttgctggg caccatcggc cttgatctct tcgagcaggg
     1861 tgcggtagat cgcagtcatg atgagcccag tgcgctgggc cttgcggtct tcgatcggca
     1921 gcgcgttgag tgcgcggtca tagaaatccg aagcgcgcgc gatctggaaa tcgagtagtt
     1981 gctggacacg gtctgattcg cggccttgca ggatgtcgcg ctcagtgaca ccgaattgcg
     2041 cgagctcgtc cagcggcagg tagatgcggt cccgccgcgc atcctcaccg acgtcacgga
     2101 tgatattggt gagctggaaa gccagcccca ggtcatgggc gtatttgagt gtattgcggt
     2161 tggtgtagcc gaagatcgcg accgagagga tgccgacgac actggcgacg cggtagcagt
     2221 agagctgcag ctgcttgaaa tcggcatagc ggttctggtc gagatccatc tccatgccgt
     2281 cgatgatc
//
LOCUS       DATMTW010000045         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_105100, whole genome shotgun sequence.
ACCESSION   DATMTW010000045 DATMTW010000000
VERSION     DATMTW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105100"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..525)
                     /locus_tag="VN084_00880"
     CDS             complement(37..525)
                     /locus_tag="VN084_00880"
                     /inference="COORDINATES: protein motif:HMM:NF018232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HWT28035.1"
                     /translation="MDSLVALGISIGVLIAAWVYVSVGMPDLGLIVWAGIVAWATFYA
                     AGGGSGGLQKTIASNLSGNFWAAVALYVFAMVGNGSVVALAIIFGVVAFIFCVQAKIS
                     LLAFIPGAFLGAATWVGVDVAGAGNDGGAVNMTDLMISASMVVGAILGYVSEAVGKKL
                     SG"
     gene            complement(696..1298)
                     /locus_tag="VN084_00885"
     CDS             complement(696..1298)
                     /locus_tag="VN084_00885"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWT28036.1"
                     /translation="MMRAWTWTVVLLLFSANVLAGAQKEEPLSNSVRALMQKSISDDA
                     APKLMFVNDPEAMKWLQEMSRRLEKRMPDPVMREDFLRTVHYEATRAGLDPQLVLGLI
                     QVESGFKKYAVSSVGARGFMQVMPFWVRSIGNPDHNLFHLRLNLRYGCTILRHYLDIE
                     RGDLYRALGRYNGSLGKPQYPNLVVGAWRKNWNYGPTAIR"
     gene            complement(1295..2980)
                     /locus_tag="VN084_00890"
     CDS             complement(1295..2980)
                     /locus_tag="VN084_00890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWT28037.1"
                     /translation="MKEAPQEAELPSHKLMLRAGLIKRLGSGLYSWMPLGLRVLRKVE
                     AVVRDEMNKAGALELLMPAVQPKELWEETGRWAVFGPQMLKIKDRHERDFCFGPTHEE
                     VITDIARKEIRSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFVMKDAYSFHTSFESL
                     EQTYQVMYQAYSNVFDRLGLKFRAVKADTGAIGGDGSHEFHVLADSGEDALAYCEQSD
                     YAANVELAEALPPSQSRSAASETMREVETPKQTACEDVAALLGIGVERTVKAIALMAD
                     GVFYLLLLRGDHTLNEIKASKLPGLGNFRFATDDEIRAALNCPPGFIGPVGVASDVKV
                     YADHTVAAMSDFVCGANKPKYHLAGVNFGRDLPEPLVIADIRNVVAGDPSPDGKGPLA
                     LCRGIEVGHIFQLRTKYAQAMNATYLDENGQTQVMEMGCYGIGVSRIVGAAIEQGNDA
                     KGIVFPAAMAPFEVVLCPIGMDRSELVKDTAQKLYAELQAAGIDVLLDDRGERPGVMF
                     ADMELIGIPHRVVLGERSLNEGKVEYQARTDTEAQALPLGDIVSRLKQVLVAT"
BASE COUNT          618 a          922 c          857 g          585 t
ORIGIN      
        1 gatcaccgcg cgtcgccgcc atcaaggcag gaagccctag cccgagagtt tcttgcctac
       61 cgcttctgac acatagccca gtatcgcacc gacgaccatt gaggcagaga tcatgaggtc
      121 ggtcatattc actgcaccgc catcgttacc tgcgcctgcg acatcgacgc ctacccatgt
      181 cgcagcaccc aggaacgcgc ccggtatgaa tgcaagcagg gatatcttgg cttgtacgca
      241 aaagatgaac gcaacgacgc cgaagatgat ggcaagggca acgacgctgc cgttaccgac
      301 catggcgaac acatacaggg cgacggctgc ccagaagtta ccggacagat tggacgcgat
      361 cgttttctgc agcccgcctg aaccgccacc ggccgcatag aacgtggccc aagctacgat
      421 cccggcccaa acgatgagcc ccagatccgg catgccgacc gagacataga cccatgcggc
      481 gatcaatacg ccgatgctga tacctaacgc tacgagtgaa tccattgtat ttcctcccaa
      541 aacgagttga agtggaacta cagcggacat aaaggcatgt ctgcagatct tgaccgtctg
      601 ctgatgcttt catgtcaagt ccgctagcgg gatagtagac tgcttttgtc gggaatgaca
      661 acctttttgc accaagactg tgcaagtggc gatggtcagc ggatagccgt ggggccgtaa
      721 ttccagttct tacgccaggc gccgacgacc agattagggt attgcggctt gcccagactg
      781 ccgttatagc ggccaagggc acggtaaaga tcaccacgct cgatatccag gtaatgccgc
      841 aggatggtgc agccgtagcg caggttgagc cgcaggtgga acagattatg gtccggattg
      901 ccgatcgagc gcacccagaa aggcatcacc tgcatgaacc cgcgcgcgcc gaccgatgag
      961 accgcgtatt tcttgaagcc gctctcgacc tggatcaagc cgagcaccag ctgcggatcc
     1021 agcccggccc gtgtcgcttc gtaatgcacg gttcgcagga aatcctcgcg catcaccggg
     1081 tccggcatgc gcttttccag tcggcgagac atctcctgca gccacttcat ggcctccgga
     1141 tcgttgacga acatgagctt gggcgccgcg tcatcgctga tggatttttg catcagcgca
     1201 cgcacgctat tggacaaggg ttcttccttc tgcgcaccgg ccagcacatt ggccgagaac
     1261 agcagcaaga cgacggtcca tgtccaagca cgcatcatgt cgcgaccaac acctgcttga
     1321 gtcgggaaac gatatcgccc agaggcaacg cctgggcttc agtatcggtg cgggcctgat
     1381 actcgacctt gccttcgttc aggctgcgct cgcccagtac cacccggtgt gggataccga
     1441 tcagttccat gtcggcgaac atcacccctg gccgctcgcc gcggtcgtct agcaatacat
     1501 cgataccggc agcctgcagc tccgcgtaca acttttgcgc ggtgtccttc accagctcgc
     1561 tgcgatccat gccgattgga cataacacaa cttcgaacgg cgccatggcg gcagggaaga
     1621 cgatgccttt ggcgtcgttg ccctgctcga tggcggcgcc aacgatgcgc gaaacgccga
     1681 taccgtagca gcccatctcc atgacctgcg tctggccatt ctcgtcgagg taagtcgcgt
     1741 tcatggcctg tgcgtatttg gtgcgcaact ggaagatatg gccgacctcg atgccgcgac
     1801 acagcgcgag cggacccttg ccgtccgggc tcggatcgcc ggcgacgaca ttgcggatat
     1861 ccgcgatgac cagcggctca ggcaagtcac ggccgaaatt gacacctgcc aggtgatact
     1921 tgggcttgtt ggcgccacag acgaagtcgc tcatggcggc gaccgtgtga tcggcataga
     1981 ccttgacgtc ggaagcgacg ccaaccgggc cgatgaaccc cggcggacag ttcaatgcgg
     2041 cacggatctc atcgtcggtg gcgaacctga aattccccag ccccggcaac ttggacgcct
     2101 tgatctcgtt cagcgtatgg tcaccccgta gcagcagcag atagaacacg ccatcggcca
     2161 tgagggcgat ggccttgacc gtgcgttcga caccgatgcc aagtagtgcc gccacatcct
     2221 cgcacgcggt ctgtttcggc gtctcgactt cgcgcatggt ctcactggcg gcagaccgtg
     2281 actggcttgg cggcagggct tctgccaact cgacgttggc cgcgtaatcg gattgctcgc
     2341 aataggccaa ggcatcttcg ccggaatcgg ccaggacgtg aaattcatgc gatccgtcgc
     2401 cgccaatggc gccggtgtcg gctttcaccg cgcggaactt gaggccgagg cggtcgaaga
     2461 cattgctgta ggcctgatac atgacctggt aagtctgttc cagcgattcg aaactggtgt
     2521 ggaaggagta ggcgtccttc atcacgaatt cacgtgcgcg catgacaccg aaacgtgggc
     2581 ggatctcgtc gcggaacttg gtctggatct ggtaaaaatt cagtggcaat tgcttatagg
     2641 agcggatctc cttgcgcgcg atatcggtga tgacttcctc gtgcgtcggg ccgaaacaga
     2701 aatcgcgttc gtgcctgtcc ttgatcttga gcatctgcgg tccgaacacg gcccagcgac
     2761 cggtctcttc ccacaactcc ttgggctgca cggctggcat cagcagttcc agcgcgcctg
     2821 ccttgttcat ctcgtcacgc accaccgcct cgaccttgcg caacacgcgc aggcccaacg
     2881 gcatccagct atagagccct gaacccagac gcttgatcag cccggcgcgc agcatgagct
     2941 tgtggctagg cagttcggct tcctgcgggg cttctttcag gg
//
LOCUS       DATMTW010000046         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_105117, whole genome shotgun sequence.
ACCESSION   DATMTW010000046 DATMTW010000000
VERSION     DATMTW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105117"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VN084_00895"
     CDS             complement(<1..531)
                     /locus_tag="VN084_00895"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HWT28038.1"
                     /translation="MIYSRIAGTGSYLPQRILTNAELEKMVDTTDEWIVTRTGIRQRH
                     IIADDEFTSDMAVAAGQRAMVAAAIDASQVDLIIIATTTPDHTFPSTACIVQQKLGIT
                     NCPAFDIQAVCSGFVYAVATADSFIKSGMAKCALVVGADSMSRITDWTDRSNCILWGD
                     GAGAVIMTASDTPVVLS"
     gene            complement(528..1583)
                     /gene="plsX"
                     /locus_tag="VN084_00900"
     CDS             complement(528..1583)
                     /gene="plsX"
                     /locus_tag="VN084_00900"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWT28039.1"
                     /translation="MDITVAIDAMGGDHGPHVTVPAALDALKQDDQINIVLVGLTEVI
                     EAELAAHKAAVGPRLRIHHASEVVTMDESPQSALKNKKDSSMRVAINLVKSGEANACV
                     SAGNTGALMATARFVLKTLPGIDRPAIAATLPSQKGMTYMLDLGANADCTPEHLLQFA
                     IMGAMLVSCVEHKDRPTVGLLNIGSEDIKGNEVVKQAGELLRASHLNFYGNVEGNDIY
                     KGTTDVVVCDGFVGNIALKTSEGLAQMMARFLTQEFKRSWMTKLMALFAMPVLKAFKS
                     RLDPRRYNGASFLGLRGIVVKSHGGADSFSFYHAIHAAAEESRNGVLRRITEQLEIEH
                     IKPSAAAEGPAAVKEVA"
     gene            complement(1657..1836)
                     /gene="rpmF"
                     /locus_tag="VN084_00905"
     CDS             complement(1657..1836)
                     /gene="rpmF"
                     /locus_tag="VN084_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWT28040.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDFLTNPPLAVEPTTGETHLRHHVSP
                     NGYYRGRKVLPSKGE"
BASE COUNT          396 a          559 c          570 g          444 t
ORIGIN      
        1 cgagagtacg acaggcgtgt cgctggcggt catgatcacg gcgccggcgc cgtcacccca
       61 caggatgcaa ttgctgcgat cggtccagtc ggtgatccgc gacatgctgt cggcaccgac
      121 caccaatgcg cacttcgcca taccgctctt gataaagcta tcggcggtgg ccacggcata
      181 gacaaagccg ctacagacag cctggatatc gaatgccggg caatttgtga tgccgagttt
      241 ctgctgcacg atgcaggcag tgctcggaaa ggtatgatcg ggcgtggtgg tggcgatgat
      301 gatgaggtcg acctgactcg cgtcgatggc agcagccacc atcgcgcgct gccccgccgc
      361 cactgccata tcgcttgtga actcgtcatc agcgatgatg tggcgctggc ggatgccggt
      421 gcgggtgaca atccactcgt ctgtggtgtc gaccatcttt tccagctcgg cattggtcag
      481 gatacgttgt ggcaggtaac tgccggtccc agcgatacgg gaatagatca tgcaacctct
      541 tttactgctg ccgggccttc ggctgcggca gacggtttga tgtgttcgat ctctagctgt
      601 tccgtgatac ggcgcaagac accattacgt gactcttctg ccgccgcatg gatcgcgtgg
      661 tagaaagaga agctatccgc accgccgtgg ctcttgacga cgatgccgcg aaggccgaga
      721 aaactggcac cgttatagcg gcgtgggtct agacggctct taaacgcctt gagcacgggc
      781 atcgcgaaca gggccatgag cttggtcatc cagctgcgct tgaattcctg ggtcaggaag
      841 cgcgccatca tctgagccag gccttcagag gtcttcaggg cgatattacc gacgaaccca
      901 tcacaaacga cgacatctgt cgtccccttg tagatgtcat tgccctcgac attcccgtag
      961 aagttgaggt ggctggcgcg tagcaactca cccgcctgct tcacgacctc attgcccttg
     1021 atgtcttcgg aaccgatatt gagcaggccg accgtgggcc gatccttgtg ctcgacacag
     1081 gaaacgagca tggcgcccat gatggcgaac tgcagcaagt gctctggggt gcaatcagca
     1141 ttagcaccca gatccagcat gtaagtcatg cccttctggc taggcagggt ggcggcgatc
     1201 gctgggcgat cgatgcctgg cagggtcttc aggacgaagc gcgcggtcgc catcagggcg
     1261 ccggtattgc cggcactgac acaggcattg gcttcgccac tcttgaccag attgatcgcc
     1321 acacgcatcg aagaatcttt tttattcttc aatgcacttt gcggagattc atccatggtc
     1381 acgacttcgc tagcgtgatg gatgcgcaag cgagggccga cagcagcctt gtgcgcagcg
     1441 agctccgcct cgatgacctc tgtgagacct accagcacga tgttgatctg gtcgtcctgc
     1501 tttaatgcgt ccaatgcagc aggaacagtg acgtgcgggc cgtgatcgcc ccccattgca
     1561 tcgatggcga cggtgatatc catattcagg atcggatttg caaaacaaaa cgccaagcca
     1621 tcacgacttg gcgcacttgt tcagtaatcg actagattac tcgcccttgg aaggcagaac
     1681 cttgcggcca cggtagtagc cgttcgggct cacgtggtga cgcaggtggg tttcgcccgt
     1741 ggtgggctca acagccagcg gcgggttggt cagaaagtcg tgcgaacgat gcatgccacg
     1801 cttggaaggc gacttcttgt tttgttgaac tgccatgatt gactcctatt cagtactctc
     1861 taaaatttcg gctctcgcca atttatcgcc cttgcgactt gagtttttcc aactcggcaa
     1921 atgggcttgc tttctttgat tcgctcagct tgcttgtcgt gccacaaac
//
LOCUS       DATMTW010000047         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_172237, whole genome shotgun sequence.
ACCESSION   DATMTW010000047 DATMTW010000000
VERSION     DATMTW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172237"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /gene="mrdA"
                     /locus_tag="VN084_00910"
     CDS             complement(<1..1409)
                     /gene="mrdA"
                     /locus_tag="VN084_00910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWT28041.1"
                     /translation="MKVRSELKNYRHEQYYFRVRLAIAAIVVLVCFGILASRFFYLQM
                     AKYDYFQTLAENNRISLVPIVPNRGLIVDRNGVVLAHNFFVYTLEITPSRVENVEETI
                     NELAKLVEVSAADRKRFKKLRAQSHSFESVPLRTHLNEVEAAHFAVNRYRFPGVEIKS
                     RLFRHYPQGQQGSHFIGYIGRINDKDLEALEESGDLSNYKGSDHIGKSGIEQYYEHDL
                     HGTTGFQQVEIDADGHAVRVLSSTAPVPGNNLVLSIDSKLQQIAEQAFGERRGAMVAI
                     NPKNGEVLAYVSMPTFDPNLFVDGIDTENWKSLNESLDKPLINRPLRGIYPPGSTFKP
                     FVALAGLEAGKREPPFSISDNGYYTLPGSSHRYRDWKAGGHGQVDVRRAITVSCDTFF
                     YGLAVELGIERLTDFVQHFGFGRKSGIDIKGEIGGLLPTPDWKMRRWKQKWYPGETVI
                     VGIGQGYTLVTPLQLAQATA"
     gene            complement(1409..1888)
                     /gene="mreD"
                     /locus_tag="VN084_00915"
     CDS             complement(1409..1888)
                     /gene="mreD"
                     /locus_tag="VN084_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWT28042.1"
                     /translation="MRPIRLGTIYLSLLAALVLQLLPWSGFGLLLRPDFVLLVLLYWL
                     LRAPQLCNIGTAWFAGLVMDLATGGLFGQYGLAYALTAYLAVSYQRRLALFTLWQQAG
                     YVFVLLLVAQLILMILKLFAGDESPGWHYFLASVTGILLWQLFIFSRLGADSHPHRS"
     gene            complement(1875..2933)
                     /gene="mreC"
                     /locus_tag="VN084_00920"
     CDS             complement(1875..2933)
                     /gene="mreC"
                     /locus_tag="VN084_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWT28043.1"
                     /translation="MQHQDPPAFFVRGPSPFARLVFFAALSLTLMATDARLHYLTEIR
                     LGLAAILHPLELLAQLPSNLYHDADEYLTTHHTLLNENRRLTGQAIAQATQLQRLRDL
                     QTENDHLRSLLGAAEASVQPARMAEIVHMGRDPFTHKVTVNLGAQHGIVAGQAVVDAA
                     GVIGQVTRAYPFNSEVTLITDKDLAIPIQIERNSLRAIAFGHGRNNTIDLPYLPANVD
                     IRQGDRLITSGIDGVYPPGLAVAQVVTIEHNPDSPFAHIVCKPIAGIENNRQVLVLSL
                     PATEPPTGKAISNKAGNKAVTPSAAPAASSATTPANAKPSAEAAKPAMAKPAMAKPSA
                     AQPRTPTTTQEGPAHATH"
     gene            complement(3037..4080)
                     /locus_tag="VN084_00925"
     CDS             complement(3037..4080)
                     /locus_tag="VN084_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWT28044.1"
                     /translation="MLGFLNSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     GGPTGKKTMLAVGLEAKRMLGRAPANITAIRPMKDGVIADFTITEQMLKYFIKKIHES
                     RLFSPNPRIIICVPVGSTQVERRAIRESAIGAGARQVYLIEEPMAAAIGADMPVAEAT
                     GSMVVDIGGGTTEVGVISLGGIVYAKSERVGGDKFDQAIIDYIRRNYGMQISEPTAEL
                     IKKKIGSAFPGSEVLEMEVTGRNLAEGLPRKCTISSNEILEALTEPLNAIVGAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLVEETGLPVIVAEDPLTCVARGSGRAL
                     EEMDKLGNVFASE"
     gene            4209..4523
                     /gene="gatC"
                     /locus_tag="VN084_00930"
     CDS             4209..4523
                     /gene="gatC"
                     /locus_tag="VN084_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWT28045.1"
                     /translation="MSLSTSDVKRIAHLARIEISDAEAEATLHKLSGILGLIEQMQAV
                     DTTGITPMSHSQDVTQRLREDVVTASNQRELFQSVAPAIGNGSDAQAVEHGLYLVPKV
                     IE"
     gene            4534..>4912
                     /locus_tag="VN084_00935"
     CDS             4534..>4912
                     /locus_tag="VN084_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWT28046.1"
                     /translation="MINDSLKALGAMLASKQISSVEMTTEFLQRIARHNPDINAYITV
                     DAEKSLAQARAADERIANGTASALTGIPIAQKDIFVAKGWKTTCGSKMLDNFIGPYDA
                     GVIERFDVAGAVNLDKTNMDEFAM"
BASE COUNT          941 a         1404 c         1589 g          978 t
ORIGIN      
        1 gcggtagcct gggctagctg caacggcgtg accagggtat agccctggcc gatgccgacg
       61 atcacggtct cgcccggata ccatttctgt ttccatcgcc gcatcttcca gtccggtgtc
      121 ggcagcaggc cgccgatctc acccttgatg tcgatgccgg atttgcggcc gaaaccgaag
      181 tgctgcacga agtcggtcag tcgttcgatg ccgagctcca cagcgagccc gtagaagaag
      241 gtatcgcagg acacggtgat cgcacgccgc acatcgacct ggccatggcc gccggctttc
      301 cagtcccggt agcggtgact gctgccgggc agcgtgtaat aaccgttgtc gctgatactg
      361 aacggcggct cgcgtttgcc cgcttcgagc ccggccagcg cgacgaaggg tttgaaggtg
      421 gagcccggcg gatagatgcc gcgcaaaggg cggttgatca ggggcttgtc cagcgattcg
      481 ttgagtgatt tccagttttc ggtatcgata ccgtcgacga acaggttggg gtcgaaggtt
      541 ggcatgctga cgtaagccag cacctcgccg ttcttcgggt tgatggcaac catggcgccg
      601 cgccgttcgc cgaaggcctg ctctgcgatc tgctgcagct tgctgtcgat ggagagcacg
      661 aggttgttgc caggcactgg ggccgtgctc gacagcacgc gcaccgcatg accgtcggcg
      721 tcgatctcga cctgctggaa gccggtggtg ccatgcaagt catgctcgta atactgttcg
      781 ataccgctct tgccgatatg gtcggagccc ttgtaattcg acaggtcacc ggattcttcg
      841 agcgcttcca ggtccttgtc gttgatgcgg ccgatgtagc cgatgaaatg cgaaccctgc
      901 tgaccttgcg ggtaatgccg gaacaggcgt gacttgatct ccacgccggg gaaacgatag
      961 cggttgacgg cgaagtgcgc ggcctcgact tcgttcaggt gcgtgcgcaa gggcacgctc
     1021 tcgaaactgt ggctttgcgc gcgcagcttc ttgaaacgct tgcggtctgc cgcactgacc
     1081 tcgaccagct tggcgagctc gttgatggtc tcttccacgt tctccacgcg ggatggggtg
     1141 atctccagcg tgtatacgaa gaagttatgg gcgagcacga cgccattgcg gtcgacgatg
     1201 agcccgcgat tggggacgat gggtaccagc gagatgcggt tattctctgc cagggtctgg
     1261 aagtagtcgt acttcgccat ctgcaggtag aagaagcggc tggcgaggat accgaaacag
     1321 accagcacga cgatggcggc gatcgccagg cgcacgcgga aatagtattg ttcgtgccgg
     1381 tagtttttaa gttcggaacg gactttcatc aactgcgatg cggatggctg tcggcaccca
     1441 gccgggaaaa gatgaacaat tgccagagca ggatgccggt gacgctggcg aggaagtagt
     1501 gccagcccgg gctttcatcg cccgcgaaca gcttgaggat catcaggatc agttgcgcca
     1561 ccagcagcag cacgaatacg tagcccgcct gttgccacag ggtgaatagc gccagacgtc
     1621 gctggtaact gacggcaaga taggcggtga gcgcgtaggc gaggccgtat tggccgaaca
     1681 ggccgccggt ggccagatcc atgaccaggc cggcgaacca ggccgtgccg atattgcaca
     1741 gctgcggcgc gcgcagcagc cagtagagta gcacgagcag gacgaaatcc ggccgtagca
     1801 atagtccgaa gccggaccac ggcaagagct gcaataccag cgccgccagc agcgagaggt
     1861 agatcgtgcc aagcctaatg ggtcgcatgc gcaggccctt cttgcgtggt cgtcggtgtt
     1921 cttggctggg ccgcagatgg tttggccata gctggtttgg ccatagctgg cttggctgct
     1981 tccgcggaag gcttggcgtt cgctggcgtg gtggccgatg aggccgccgg cgcggcagac
     2041 ggcgtcaccg ccttgttgcc ggctttattg ctgatcgcct tgcccgtcgg cggttcggtc
     2101 gcgggcaagc tcagcaccag cacctgccgg ttgttctcga tgccggcgat gggtttgcag
     2161 acgatatggg cgaaaggcga atccggatta tgttcgatgg tcaccacctg ggcgacggcg
     2221 agccccggtg gatagacgcc atcgataccg gatgtgatca ggcggtcgcc ctggcggata
     2281 tcgacattcg ccggcaagta gggcaggtcg atggtgttgt tgcgcccgtg cccgaaagcg
     2341 atggcgcgca ggctgttgcg ctcgatctgg atggggatcg ccagatcctt gtcggtgatc
     2401 agcgtcactt cgctattgaa ggggtatgcg cgcgtcacct ggccgatgac gcccgcggca
     2461 tcgaccacgg cctggccggc gacgatcccg tgctgtgctc ccagattcac ggtcacttta
     2521 tgtgtgaagg gatcgcgccc catgtgcacg atctccgcca tgcgcgctgg ctgcaccgat
     2581 gcctcggccg cacccagcag gctgcgcaga tggtcgttct cggtctggag gtcacgtaga
     2641 cgctgcaatt gcgtcgcctg cgcgatcgcc tggccggtca ggcggcgatt ctcattgagc
     2701 agcgtatggt gcgtggtcag gtattcgtcg gcgtcgtgat agaggttgct gggtagttgc
     2761 gccagcagtt ccagtgggtg caggatggcc gccaggccga ggcggatctc agtcaggtaa
     2821 tgcaggcggg catcggtcgc catcagggtc agcgacaagg cggcaaagaa caccaggcgg
     2881 gcaaaaggac tggggccacg gacgaagaag gccggcgggt cctgatgttg catggcactt
     2941 ggcatctgaa tctagggcct tgtttcctga aaggctggct gacaaaaagc aggccattca
     3001 tcatggcctg cagggtgatc gaatgatgag gtggccttat tcgctggcaa atacattgcc
     3061 cagtttgtcc atttcttcca gtgcgcggcc actgccgcgt gcgacgcagg tgagcgggtc
     3121 ttctgcgacg atcaccggca ggccggtctc ctccaccagc aagcggtcga ggtcacgcag
     3181 cagcgcgcca ccgccagtga gtaccatgcc cttctcggcg atatcggcac cgagttccgg
     3241 tggggtctgt tccagcgcgg acttgaccgc gccgacgatg gcgttgagcg gctcggtcag
     3301 cgcttccagg atctcattgc tggagatggt gcatttgcgc ggcaggcctt cagccaggtt
     3361 gcgaccggtg acttccatct ccagcacttc ggagccgggg aaggcggagc cgatcttctt
     3421 cttgatcagt tcggcggtcg gttcggagat ctgcatgccg tagttgcggc ggatgtaatc
     3481 gatgatggct tgatcgaact tgtcgccgcc gacacgctcg gatttggcgt agacgatgcc
     3541 gccgagtgag atgacgccga cctcggtggt gccgccgccg atgtcgacga ccatggagcc
     3601 ggtcgcttca gccactggca tatcagcgcc gatcgcggct gccatcggct cctcgatcaa
     3661 atagacttgc cttgcgccgg cgccgatcgc ggattcgcgg atggcacggc gttcgacctg
     3721 agtggagccg accggcacgc agatgatgat gcgtggattc ggcgagaaca ggcgggattc
     3781 gtggatcttc ttgatgaaat acttcagcat ctgttcggtg atggtgaaat cggcgatcac
     3841 gccgtccttc atcgggcgga tggccgtgat gttggccggc gcgcggccca gcatgcgctt
     3901 ggcctcgagg ccgacggcga gcatggtctt cttgccggtg gggccgcctt cctggcggat
     3961 ggcgacgacg gacggctcgt cgagcacgat acctttgcca cgtacgtaga tcagggtgtt
     4021 tgcggtgccg aggtcgatgg caagatcatt ggagaaataa ctattgagaa aaccaagcat
     4081 tgtttttgta tcccgagcga gctgtcatga agcgcatccg gctaaagatg tgcttaaaat
     4141 caagttataa taactgatat cgcattgaaa tttaagattc aaccgaaatt attggaacgg
     4201 gtcgccccat gtcactgagc acttccgacg tcaagcgcat cgctcacctc gcacgcatcg
     4261 aaatcagcga tgccgaggcc gaagcgacct tgcacaaact ttccggtatc ctcggcctca
     4321 tcgagcagat gcaggcggtg gacaccaccg gcatcacgcc catgtcgcat tcgcaggacg
     4381 tcacgcagcg cctgcgtgaa gacgtggtga cggccagcaa ccagcgcgaa ttgttccagt
     4441 cggtcgcgcc cgcgatcggc aacggttcgg acgcgcaagc ggtcgagcac ggcctctacc
     4501 tcgttcccaa agtcatcgaa taaacgtcag cacatgatca acgacagcct caaagcgctg
     4561 ggggcgatgc ttgccagcaa acagatctcc agtgtggaga tgaccaccga attcctgcag
     4621 cgtatcgcgc gtcacaaccc ggatatcaac gcctacatca cggtcgatgc cgaaaagagc
     4681 ctggcccagg ccagggcagc ggacgaacgt atcgcaaatg gcacggcatc ggccttgacc
     4741 ggcattccca tcgcacagaa ggacatcttc gtcgccaagg gctggaagac cacctgcggt
     4801 tcgaaaatgc tcgataactt catcgggcct tacgatgccg gtgtcatcga gcgcttcgat
     4861 gtggctggcg ccgtcaatct cgacaagacc aacatggacg agttcgccat gg
//
LOCUS       DATMTW010000048         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_205806, whole genome shotgun sequence.
ACCESSION   DATMTW010000048 DATMTW010000000
VERSION     DATMTW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205806"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN084_00940"
     CDS             <1..228
                     /locus_tag="VN084_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28047.1"
                     /translation="VIPNDAVVWYGGKAWVYRQESPERFVRQPIETDHETGNGWFNTG
                     LIKPGDALVTSGAQLLLSEEFKYQIKNENED"
     gene            367..3498
                     /locus_tag="VN084_00945"
     CDS             367..3498
                     /locus_tag="VN084_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWT28048.1"
                     /translation="MMSALVRFSIRFNGVIIGLASLVVLYGLYSLTRSNLDVFPEFSP
                     TQLVIQTESPGLSAELVEQLVSQPIEQSIAGTVGIELMRSQSIPGLSVVTIIFEEGTD
                     VFRNRQVIAERLATLANRLPRGITPNITPLTSAASTVMGIGLTSDQRSLMELRTLVDW
                     SIRPHLLAVQGVADVNTFGGEVRQFQIQADPEKLIRYGISLQQVMDAADKATGVRGAG
                     FIENNNQRIIINSEGQALTPAMLAQTTLLYRDGQTVRIGDIGKVVEGAAPSISSAQIN
                     GKTGIYLSIQGQLGANTHGVTQALEKALREIEPSLKDEGVTLHKGLFRPANFIETAID
                     GVRADILIGSVLVITVLFLFLFNARTAFISATAIPLSLLTAIVVLSYFNIGLNIMVLG
                     GLAIALGEVVDDAIIDTENIFRRLRENRLLPNPLATHRVVFNASMEVRSSVVYATFIV
                     ALVFMPLLTLSGVAGKLFAPLGLSYIAAILASLAVALTLTPALCYLLLGNAKLDSEDP
                     PLIRSIKQRYVKLLYRIERHYRLVIGVATVLIALGLGILPLFKSQFIPNLYEGHYIMH
                     MTAVPGTSEQESLRIGKKVTETIMGIKGVKSVVQWVGRAPNAADTFGTHYSEFEIEIG
                     TLSGEEQTRILNEIREQLAGEAEDEDGDGVAEPGFVGVNFAINTFLTERIEETISGYP
                     ASVVINIYGRDLDALDRDAQAVASVLASIDGATDVLVQSPPGTPQLVIRLRNERLLQW
                     GLQPTDVLDSIRAAYESVPVTQVYDGNRVIGVSVVLAEPQRNDITQVAKLPLKNIEGR
                     IVRLGDVADINQENGRSKILHAGAKRIQTVTANVTNRDIQQFAKEMKSRIRETVKLGP
                     GNYMTFSGEAEAQAKSREDLIVHSLLAGVGIFLMLYVAFGRLRNLLLTFANLPFALIG
                     GVLAALFTGGWISLGSLVGFVTLFGITLRNSIMMISHYQHLVDEEGMTWGLETAIRGA
                     SERLPSILMTALVTALGLLPLAAGSGQPGREIEGPMATIIVGGLVTSTILNLLILPTI
                     MLHFGRFEKRLQD"
     gene            complement(3521..>3726)
                     /locus_tag="VN084_00950"
     CDS             complement(3521..>3726)
                     /locus_tag="VN084_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWT28049.1"
                     /translation="WLILSDIAEHLGLRTRETLLAMFEAAGLQVISRMDTRPLHGKAT
                     DTADPLHAARAAEVTSLWRLSLA"
BASE COUNT          749 a         1185 c         1065 g          727 t
ORIGIN      
        1 gtcatcccga acgatgccgt ggtctggtat ggcggcaagg cttgggtcta ccgccaggag
       61 agcccggaac gcttcgtgcg ccagccgatc gagactgacc acgaaacagg caacggctgg
      121 ttcaataccg gcctgatcaa gcccggcgac gccttggtga cgagtggtgc ccagctgctg
      181 ctgtcggaag aattcaagta tcagatcaag aatgaaaacg aggattaaat tgttcaattt
      241 aatcctcgtt tcggtggaga ctcatgcgcg cagcgcgtga tgtcgtagcc aaaatgagga
      301 cgtccagata gcggaagcaa ctatgctcta tattcaaaaa aacaaatcaa gcactgagct
      361 ttcagtatga tgtccgccct cgtccgcttc tcgatccgct tcaatggcgt catcatcggc
      421 cttgccagcc tggtcgtact ctatggcctt tatagcctca cacgcagcaa tctcgatgtg
      481 tttccggagt tctcgccgac ccaattggtg atccagaccg aatcacccgg actttccgcg
      541 gaactggtcg agcagctggt cagccagccg atcgagcaga gtatcgccgg cacggtgggt
      601 atcgagctca tgcgctcaca gtcgatcccc ggcctgtcgg tggtcaccat catcttcgaa
      661 gaaggcactg acgtcttccg caaccgccag gtcatcgccg agcgcctcgc cacgctggcg
      721 aatcgcttgc cacgtggcat cacgccgaac atcaccccgc tgacctccgc cgcgagcaca
      781 gtcatgggta tcggcctcac gtcggaccag cgctcgctga tggagctgcg tacgctggtc
      841 gactggtcga tccggccgca cttgctggcg gtgcaaggag tcgccgatgt gaacaccttc
      901 gggggtgagg tccgccagtt ccagatccag gcggatccgg aaaagctcat ccgctacggc
      961 atctcactgc agcaagtgat ggacgccgcc gacaaggcca caggcgtgcg tggcgctgga
     1021 ttcatcgaga acaacaatca gcgcatcatc atcaacagcg aaggccaggc gctgaccccg
     1081 gccatgctgg cgcaaaccac cctgctctat cgcgatggcc agaccgtccg tatcggcgac
     1141 atcggcaagg ttgtcgaagg cgccgcgccc tccatcagca gcgcccagat caatggcaag
     1201 accggcatct atctgtcgat ccagggccag ctgggcgcca acacgcatgg cgtgacgcag
     1261 gcgttggaga aggccctgcg ggagatcgaa ccttcactga aggacgaagg cgtgaccctg
     1321 cataaaggcc tgttccgccc cgccaacttt atcgaaaccg ccatcgatgg cgtacgcgcc
     1381 gacatcctga tcggttcggt actggtgatc acggtcctgt tcctgttcct gttcaacgca
     1441 cgcaccgcct tcatctcggc cacggccatc ccgctgtcgc tgctgaccgc catcgtggtg
     1501 ctcagttatt tcaacatcgg cctcaatatc atggttctcg gtggtcttgc catcgcgctg
     1561 ggcgaagtgg tcgatgacgc catcatcgac accgagaaca tcttccgtcg cctgcgcgag
     1621 aatcggctgt tgccgaatcc tctagcgact caccgcgtgg tattcaacgc ctcgatggaa
     1681 gttcgcagct ctgtggtcta tgccacgttc atcgtggccc tggtcttcat gcccttgctc
     1741 acgctcagcg gcgtcgccgg caaactgttc gcgccactcg gcctatccta tatcgccgcc
     1801 atcctggctt cgctcgctgt agcgctgaca ctgacgcctg cgctgtgcta cctgcttctg
     1861 gggaacgcaa agctcgatag tgaggatccg ccgctgatcc gtagcatcaa gcagcgttac
     1921 gtgaagctgc tgtatcgcat cgagaggcac tatagactgg tgatcggtgt cgccaccgtc
     1981 ctcatcgccc tggggcttgg catcctgcca ctgttcaaga gtcagttcat ccccaacctc
     2041 tacgaaggcc attacatcat gcacatgacg gccgtgcccg gtacgtctga gcaagagtcg
     2101 ctgcgtatcg gcaagaaggt gactgaaacc atcatgggca tcaaaggcgt gaaatccgtg
     2161 gtgcagtggg taggccgcgc accgaatgcg gcagatacgt tcggcacgca ttacagcgag
     2221 ttcgagatcg agatcggcac gctgtccggc gaggaacaga cccgcatcct caacgagatc
     2281 cgcgagcaac tggcaggcga ggcagaggat gaagacggtg acggcgtcgc cgaaccgggt
     2341 tttgtcggcg tcaatttcgc catcaataca ttcctcaccg agcgtatcga agagacgatc
     2401 tccggttacc cggcgtctgt cgtcatcaat atctacgggc gtgacctgga cgcgctcgac
     2461 cgcgacgcgc aagcggtcgc cagcgtcctc gccagcatcg acggcgccac tgacgtgctc
     2521 gtgcaatccc cgcccggcac gccgcagctg gtgatccgcc tgcgcaacga gcgcctattg
     2581 caatggggtc tgcaacccac tgatgtactg gatagcatcc gcgccgccta cgaaagcgtg
     2641 cccgtcaccc aggtctatga cggcaatcgg gtcatcggcg tcagtgtggt gctcgccgag
     2701 ccgcaacgca acgacatcac ccaggtcgcc aagctgccgc taaagaacat cgaaggccgt
     2761 atcgtgcgtc taggcgatgt cgccgatatc aaccaggaga acggccgctc caagatcctg
     2821 catgcgggcg cgaaacgtat ccagacggtc accgccaacg tcacgaaccg tgacatccag
     2881 cagttcgcga aagagatgaa gtcgcgcatc cgcgaaacgg taaagctggg tccgggcaat
     2941 tacatgacct tctcgggcga agcggaagcc caggccaagt cgcgcgagga tctgatcgtg
     3001 cactccctgc tcgccggcgt cggcatcttc ctcatgctgt atgtcgcctt cggtcgactg
     3061 cgcaatctct tgctgacttt cgccaacctg cccttcgccc tgatcggcgg ggtgctggcc
     3121 gcactattta ccggtggctg gatatcgctg ggctcgctgg tcgggttcgt cacgctgttc
     3181 ggcatcaccc tgcgcaactc catcatgatg atctcgcact accagcacct ggtagacgag
     3241 gaaggcatga cctggggact ggaaacggcg atccgcgggg cttccgaacg cctgccgtcg
     3301 atcctcatga cggcactggt caccgcgctt ggcctactgc cgctcgcggc cggcagtggt
     3361 cagccgggcc gtgagatcga aggcccgatg gctaccatca tcgtcggtgg gctggtgact
     3421 tccacgatcc tgaacctgct gatcctaccc accatcatgt tacatttcgg gcgctttgaa
     3481 aaacgcttgc aggactaatt cacgtcggcc cagaccggat ctaagccaag cttagccgcc
     3541 agagcgatgt gacctcggcg gcgcgcgccg cgtgcagcgg atcggctgta tccgtcgcct
     3601 tgccgtgcaa cgggcgcgta tccatgcggg agatcacttg cagtccagcc gcctcgaaca
     3661 tggccagcaa ggtttccctt gtccgcaacc ccaaatgctc ggcgatatcc gagaggatca
     3721 gccagc
//
LOCUS       DATMTW010000049         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_440210, whole genome shotgun sequence.
ACCESSION   DATMTW010000049 DATMTW010000000
VERSION     DATMTW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440210"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VN084_00955"
     CDS             <1..597
                     /locus_tag="VN084_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWT28050.1"
                     /translation="PSGSGKSTFMNILGCLDKPSSGEYTLDGHQTATLDGDALARVRN
                     KTIGFVFQGFNLLSRSSLLDNVSLPLVYAGVDKAERHARAAVLLRKVGLERYFQSMPN
                     QISGGQQQRVAIARALINDPSLILADEPTGNLDSKTSEEIMALFGELNAEGITIVLVT
                     HEQDIAEHARRQVRFLDGRIVQDTQHGFGELTADVGAA"
     gene            597..1820
                     /locus_tag="VN084_00960"
     CDS             597..1820
                     /locus_tag="VN084_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWT28051.1"
                     /translation="MFGAMLGEAWHAMGANRLRTFLTMLGMVIGVGAVVLMMAIGQGA
                     QESVKESINSMGSNLFIVLSGSTTANGARIGTGNSPSLKVGDAEAIGELEGISAVAPV
                     TMGTAQIVFGSNNWNTSILGTMPSYFDVRAWTLADGYVFSDSDIRSATRVAIIGKTVA
                     QNLFGDEIDPIGKTIRIKQTPFTVVGLLDSKGQSLDGRDQDDTIVVPLTTAQRKLFGS
                     QFPGSVRMIMVQAETAEVMPVVEVDINNLLRQRHNIREGNDNDFSVRNLTSLANSAAE
                     TTRTMALLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARERDILMQFLL
                     EAIVISFFGCLIGLVLGIGGAILVGMLTQATILISGNSILLAFSVAAAVGIFFGFYPA
                     RKAAQLNPIEALRYQ"
     gene            1962..>4035
                     /locus_tag="VN084_00965"
     CDS             1962..>4035
                     /locus_tag="VN084_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWT28052.1"
                     /translation="MPLKNLPKRIANLRPGRTALGYALFGLLWVAIENRLFSPPAEHT
                     TSALLAYLLPDISLVLLSATIIYLLLYHRPASAALPASPPTGQPLHRGYLLLLLVILL
                     AVSPLITLGVKYFYLPRIEQDAYVNLQLVSRIKVVQFERWRDSRVGDAEVLAADRGFA
                     ASTAALLREDTPSHRAAIAQRFAALISAYEYESIALLDAQGNELLVQGRSVASNTIQR
                     QLTAQSRSGKVVHSDLYTIGERVQLDIVAPIIKPAATGLASTIRDPSPDIVAYVIVHT
                     GIDRQILPRVLSWPSSSSSARIVIARQADGQEALIHYQTGTPRKTTFTLRPLTASSPY
                     ASAKRTLGIDYRQHEAYVNIEPIQGSAWRMVGQIDKSEVMAPLDTLLAWIASLTLIAV
                     ALLIGAILLLLRQQQRTHALELLTESSERDRLLKRFYDMPFIGMAVIDIESKRWTQVN
                     DRLCEMLGYEAHELLRMTWIDITHPEDLERDLELNQAFIDGKLDHILIEKRLLHRSGK
                     SLIVRIEANAIRDLHGKIEHYLVAVEDITERRTAENALRQQEEFNRVLLNNQADGVVA
                     CDADMRLVVFNNIARQWHGLDPREIPPEQWSHYFGLYDASGEHELQAHEIPLVRAFNG
                     EQLQHAGMVIKAQGQAPRFVSCSATAFFDDQGKKLGAVAIMRDITEIQAQDRALRESE
                     TLFQALARV"
BASE COUNT          806 a         1318 c         1156 g          755 t
ORIGIN      
        1 ccctccggtt ccggcaagtc caccttcatg aatatcctcg gctgcctcga caaaccaagc
       61 agcggcgaat acacactgga cggccaccag accgccacgc tggatggcga tgccttggcc
      121 cgcgtccgca acaagaccat cggcttcgtc ttccagggct tcaacctgtt atcgcgttca
      181 agccttctgg acaacgtttc gctcccactg gtgtatgccg gcgtcgacaa ggcagaacgc
      241 catgcccgtg cggccgtact gctgcgcaaa gtcggactgg agcgctattt tcagtccatg
      301 cccaaccaga tctcaggcgg tcagcagcaa cgcgtggcca tcgcgcgtgc gctgatcaac
      361 gaccctagcc tgatcctcgc cgacgagccg accggtaacc tcgacagcaa gaccagcgaa
      421 gagatcatgg ccttgttcgg cgaactgaat gcggaaggca tcaccatcgt gctggtcacg
      481 cacgaacagg acatcgccga acacgcacgc cgccaagtgc gattcctcga tgggcggatc
      541 gtgcaggata cgcaacatgg cttcggtgaa ttgaccgccg acgtgggagc cgcctgatgt
      601 tcggcgcgat gttgggcgaa gcatggcatg ccatgggcgc caatcgcctg cgcaccttcc
      661 tcaccatgct cggcatggtg atcggtgtcg gcgccgtggt gttgatgatg gccatcggcc
      721 agggcgccca ggaatcggtc aaggaaagca tcaactccat gggcagcaac ctgttcatcg
      781 tgctgtctgg ttcgaccacg gcgaacggtg cccgcatcgg taccggcaac tcgccttcgc
      841 tgaaggtcgg cgatgccgag gccatcggcg aactggaagg catttcggcc gtcgcaccgg
      901 tgaccatggg gaccgcgcag atcgtgttcg gctccaacaa ctggaatacc agcatcctcg
      961 gcaccatgcc gagttacttc gatgtgcggg cttggacact ggccgatggc tatgtcttct
     1021 cagactcgga catccgctcc gccacccggg tcgccatcat cggcaagacc gtcgcccaga
     1081 acctcttcgg cgacgagatc gacccgatcg gcaagaccat ccgcatcaag cagacgccat
     1141 tcaccgtcgt cgggctgctc gacagcaagg gccagagcct cgacgggcgc gaccaggacg
     1201 ataccatcgt cgtgccgctg acgaccgcgc aacgcaagtt gttcggcagc cagttcccgg
     1261 gcagcgtgcg catgatcatg gtgcaggcgg agaccgccga agtcatgccc gtcgtcgaag
     1321 tcgacatcaa caacctgctg cggcaacgcc ataacatccg cgaaggcaat gacaacgatt
     1381 tctcggtgcg caacctgacc tcgctcgcca attcagccgc ggaaacgaca cgcaccatgg
     1441 ccctgctact gggggcgatc gcctccgtct cgctgctggt cggcggtatc ggcatcatga
     1501 acatcatgct ggtctcggtc accgaacgta cacgtgagat cggtatccgc atggcgatcg
     1561 gggcacgcga acgggatatc ctgatgcaat tcctgctgga ggcaatcgtg atctcattct
     1621 tcggctgcct gatcggcctc gtgctcggca tcggcggcgc tatcctcgtc ggcatgctga
     1681 cccaggcgac catcctcatc tccggcaact ccatcctgct ggcgttcagt gtcgccgcag
     1741 ccgtcggcat cttcttcggc ttctatccgg cccgcaaggc agcccagctc aacccgatcg
     1801 aagccctgcg ctaccaatag attcgacaag ccttgccggc agcctgctgc cctagtctga
     1861 cggcctgttt ccagcgcctg cttttctcgg caggcgttat gcattctgaa ttgtcgaatg
     1921 caccaaatct gcgcataatg aagtcaaagc acggactcat catgccgtta aaaaacctgc
     1981 ccaaacgcat cgccaactta cgcccgggga gaaccgcgct aggctacgcg ttgttcgggc
     2041 tgctttgggt cgcgatcgag aatcggctgt tctcgccacc ggccgaacat accacctccg
     2101 ccctactcgc ttatttgctg ccagacatca gcctggtgct attgagcgcg acgatcatct
     2161 acttgctgct ctaccatcgc cctgcgtctg ccgccctgcc agcatccccg cccaccggac
     2221 aaccattgca ccgcggctat cttttactgc tgctggtaat cctgcttgcg gtctctccac
     2281 tgattacgct cggcgtcaaa tacttctacc tgccgcggat cgaacaggac gcttacgtca
     2341 atctgcaact ggtgtcccgc atcaaggtcg tgcagttcga gcgctggcgg gattcgcgcg
     2401 tgggcgatgc cgaggtactg gccgcggatc gtggctttgc cgcatcgacg gccgccttgc
     2461 tgcgggagga tacgccgagc catcgggcag cgatcgcgca gcgcttcgcg gccctgatct
     2521 cggcctatga atatgaatcg atcgctttgc tcgacgcgca aggcaatgag cttctggtgc
     2581 aaggccgcag cgtggcttcc aatacgatcc agcgacagct gacggcgcag agccgcagcg
     2641 gcaaggtcgt acacagcgac ctttacacca tcggcgaacg cgtacagctt gatatcgtgg
     2701 cccccatcat caaacccgca gccaccggcc tggcatcgac gatccgggat ccgtcgccgg
     2761 atatcgtcgc ctatgtcatc gtgcataccg gcatcgatcg gcagatcctg ccgcgcgtgc
     2821 tttcctggcc cagttcgagt tcgagcgcgc gcatcgtcat cgctcgccag gccgatggtc
     2881 aggaagcgct gatccattac caaacaggca ctccgcgcaa gaccacattc acgctgcgac
     2941 cgctgacggc cagtagcccc tatgccagcg cgaaacgcac actgggtatc gactatcgcc
     3001 agcatgaggc ctatgtgaac atcgagccca tccagggttc tgcctggcgc atggtcggcc
     3061 agatcgacaa gtctgaagtg atggcgccgc tggatacctt gctggcctgg atcgccagcc
     3121 tgaccctgat cgccgtcgcc ctgctgatcg gcgccatatt gttgctgttg cgtcagcagc
     3181 agcgcacgca tgcgctggaa ttattgaccg aaagcagcga acgcgatcgc ctgctgaaaa
     3241 gattctacga tatgccgttc atcggcatgg cggtgatcga tatcgagagc aaacggtgga
     3301 cacaggtcaa tgaccgttta tgcgagatgc tgggctacga agcgcacgag ctgttgcgca
     3361 tgacctggat cgacatcacc catccggaag acctcgaacg cgacctggag ctcaaccagg
     3421 ccttcatcga cggcaagctc gaccatatcc tgatcgagaa gcggctgctg catcgatcag
     3481 gcaagagcct catcgtgcgt atcgaggcca atgccatccg cgacctgcac ggcaagatcg
     3541 aacattacct ggtcgccgtc gaggacatca ccgaacgccg cacggcagag aatgcactgc
     3601 gccagcagga ggaattcaac cgcgtactgc tcaacaacca ggcagacggc gtggtggcct
     3661 gcgacgctga catgcgtctg gtggtcttca acaacatcgc gcgccaatgg catggcctcg
     3721 atccgcgcga gatcccgccc gagcaatgga gtcattactt cggtctttac gacgccagtg
     3781 gcgagcacga actgcaggcg cacgagatcc cgctggtgcg tgccttcaac ggcgagcagc
     3841 tgcagcatgc cggcatggtg atcaaggccc agggccaggc gccgcgcttc gtctcctgca
     3901 gcgcgacggc tttcttcgac gaccagggca agaagctggg cgcggtcgcc atcatgcgcg
     3961 acatcaccga gatacaggcg caagaccgtg ccctgcgcga gagcgagacc ctgttccagg
     4021 cattggcgcg ggtcg
//
LOCUS       DATMTW010000050         1856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_474344, whole genome shotgun sequence.
ACCESSION   DATMTW010000050 DATMTW010000000
VERSION     DATMTW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474344"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VN084_00970"
     CDS             complement(<1..323)
                     /locus_tag="VN084_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class I"
                     /protein_id="HWT28053.1"
                     /translation="MDLKNFLADHALAALVADIAEACRQTATQVRKGALLGQMGSLGS
                     QNVQGETQKQLDVIANDIWLQVNERHGHYAGMASEELEAPYAVPPQYRQHGRYLLLFD
                     PLDGSS"
     gene            complement(326..865)
                     /locus_tag="VN084_00975"
     CDS             complement(326..865)
                     /locus_tag="VN084_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWT28054.1"
                     /translation="MSLSTAASVSGDLRSSRKARQFAVAACMTMSLCAPGVQAAEDQA
                     RPDYGPDITLDTAKQLAAGAVGECRQQHWKVAVAIVNTHGSLVYYERMDNTQSASARI
                     AVDKASAAAMYRRTTREFADTVNKSGPAVTTLPGVIASPGGLPVMRDGKVIGGIGVSG
                     MTGDQDEQCAKAGLANLNP"
     gene            complement(862..1209)
                     /locus_tag="VN084_00980"
     CDS             complement(862..1209)
                     /locus_tag="VN084_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWT28055.1"
                     /translation="MNYRGSCHCGQVAFEVEGEIQGAMACNCSICVRKGTLMWFVPRE
                     KLHLLTPDDAASTYTFNKHLIQHRFCPTCGIHPYAEATGPDGKPMVAINLRCIEDIDL
                     DAIPVKHYDGRSK"
     gene            complement(1256..1603)
                     /locus_tag="VN084_00985"
     CDS             complement(1256..1603)
                     /locus_tag="VN084_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWT28056.1"
                     /translation="MLFAAERTLLAWNRTSLALIAFGFVVERTGLLMRILAPERAGNL
                     TMSLTFWIGEALILLGALCAAYSSRQYTAVLKTLNPDEFPPGYAAKWGMLVNITVAVL
                     GLVLIAALYAGHD"
BASE COUNT          323 a          583 c          575 g          375 t
ORIGIN      
        1 gacgagccgt cgagcggatc gaacagcagc aggtaacgcc cgtgctgacg gtattgcggc
       61 gggacggcat aaggagcctc cagctcttcc gaagccatgc cggcgtaatg gccatggcgc
      121 tcgttcacct gtagccagat atcgttggcg atcacgtcga gctgtttctg cgtctcgccc
      181 tgcacgttct gcgaacccag gctgcccatc tggccgagca gcgcgccttt gcgcacctgc
      241 gttgcggtct gccggcaggc ctcggcgata tcggcgacca gcgctgccag cgcgtgatcc
      301 gccagaaagt ttttcaggtc catcgtcatg gattcagatt cgcgaggccc gccttggcgc
      361 attgttcgtc ctggtcgccg gtcatgccgc tgaccccgat acccccgatc accttgccgt
      421 cacgcatgac gggcaggccg ccgggcgacg cgatcacccc gggcaaggtc gtcactgccg
      481 ggccggactt gtttacggta tcggcgaatt cgcgcgtggt acggcgatac atcgccgccg
      541 cgctggcctt gtccaccgcg atcctggcgc tggcggattg cgtgttgtcc atgcgttcgt
      601 agtagaccag cgagccatgg gtgttgacga tggcgacggc gactttccag tgctgctgcc
      661 ggcactcgcc aacggcccct gcagcaagct gcttggcggt gtcgagcgtg atgtcggggc
      721 cgtaatcggg cctggcttga tcttctgccg cctgcacgcc tggcgcgcat agcgacatgg
      781 tcatgcaggc cgccacggca aactgccttg ccttgcgcga agagcgcagg tcgccggaga
      841 cgctggccgc tgtcgacaga ctcattttga acgcccgtcg taatgcttca cggggatggc
      901 gtcgagatcg atgtcttcga tgcagcgcag gttgatcgcc accatgggct tcccgtctgg
      961 tccagtggcc tcggcgtagg gatggatgcc gcaggtcggg caaaagcggt gctggatcag
     1021 atgcttgttg aaggtatagg tgctcgccgc gtcatcaggc gtcagcaggt gcagtttttc
     1081 acgcggcacg aaccacatga gcgtgccctt gcgcacgcag atcgagcagt tgcaggccat
     1141 ggcgccctgg atctcgcctt ccacttcaaa cgccacctgg ccgcaatggc agctaccacg
     1201 atagttcata ccggtctcct ttgtcgttga cttcgtcttg tctcaagccg cccgatcaat
     1261 cgtggccggc atacaaggcg gcgatgagca caaggcccag caccgccacc gtgatattca
     1321 ccagcattcc ccacttcgcg gcatagccgg gcgggaattc atccggattc agggtcttca
     1381 gcaccgccgt atattgtcgg gacgaatagg cggcacacag cgcgcccagc aggatcaagg
     1441 cctcgccgat ccagaaagtc aggctcatgg tgaggttgcc ggcgcgctcc ggcgccagga
     1501 tgcgcatcag cagaccggtg cgctcaacca caaacccgaa agcgatcagc gcgaggctgg
     1561 tccggttcca ggcgagtaag gtgcgttccg ccgcgaataa cactcttggg tctttcaggt
     1621 ctgacagtgt atcgatcacc tatgacatgg gtggataggt tcggcatgcg ccatgccgga
     1681 tttgcgtgct gcgccgcttt gcaatcaccg gtagcgcaaa gtcagggttc aggcgtgccc
     1741 gtatcctgat caccccggtc gatatcatcg cgggccgcag tggcgagcgc ttggtatttg
     1801 cgcataaagc tatccagcgt ctcttcctcc agctcttcca gatcgagcaa cgcgtt
//
LOCUS       DATMTW010000051         9539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_239098, whole genome shotgun sequence.
ACCESSION   DATMTW010000051 DATMTW010000000
VERSION     DATMTW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239098"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="coaBC"
                     /locus_tag="VN084_00990"
     CDS             complement(<1..575)
                     /gene="coaBC"
                     /locus_tag="VN084_00990"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWT28057.1"
                     /translation="MAHPSIKKLLLGVTGGIAAYKSAELVRLLVKQGIDVQVVMTAAA
                     QHFITPVTMQALSGKRVFTDMWDASIANGMPHIELSREADAILIAPASADFMAKLVHG
                     GADDLLSTLCLARECPLMVAPAMNRQMWENPATQRNIAQLRQDGVHVLGPDSGEQACG
                     ETGMGRMLEPHQLLDMIAIALQPKILAGQRVL"
     gene            625..1299
                     /gene="radC"
                     /locus_tag="VN084_00995"
     CDS             625..1299
                     /gene="radC"
                     /locus_tag="VN084_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWT28058.1"
                     /translation="MAITDWPASERPREKLLQHGAGVLSDAELLAIFLRVGVTGKSAV
                     DLARDLLTRFGSLNGIFAASPSEIGAVHGMGVSKYAQLQAIFEMSRRALAEQMRESDV
                     LGSPKQVRDYLCLRLGQMKREVFIVLFLDAQNRVVAQEEMFSGTLTQTSVYPREIVKR
                     ALHHNAASVIFAHNHPSGVAQQSRADELLTDALKQALALVDIRTLDHIVIAGNTSLSF
                     AEQGLL"
     gene            complement(1307..2167)
                     /locus_tag="VN084_01000"
     CDS             complement(1307..2167)
                     /locus_tag="VN084_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HWT28059.1"
                     /translation="MKPPKRLQALIEDGLIDEVSRQLMSGKEATVYVVRCGDEVRCAK
                     VYKEANQRSFRQNVDYTEGRKTKNSRQARAMQKGSRYGREVQEQAWQSAEVDALYRLA
                     SAGVRVPQPYNFYEGVLLMELVTDVDGHAAPRLNDVELSPEEARKHHATLITEVVRML
                     CAGIIHGDLSEFNILLGSEGPVIIDLPQAVDAAGNNQAQALLERDIDNLSHYFGRFAP
                     ELRSSQYGKEIWSLYTHGALTPDTLLTGHFRESSAPVDLKTVMRVINDVKKEEEARIR
                     RLAESQNDER"
     gene            complement(2236..2613)
                     /locus_tag="VN084_01005"
     CDS             complement(2236..2613)
                     /locus_tag="VN084_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWT28060.1"
                     /translation="MKAQIRQADPEREFFTPERCYILELSNIPEDAGLSIARARVLPG
                     VSTRWHRVIDTAERYVILEGHGQVEVKGLGSQTVMPGDVVIIPAGCPQRITNPGDSDL
                     VFLALCTPRFQQANYQDIEDSCA"
     gene            complement(2660..2980)
                     /locus_tag="VN084_01010"
     CDS             complement(2660..2980)
                     /locus_tag="VN084_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28061.1"
                     /translation="MKTFLSLVMLCILVSQAALADDSPAPADAAPIQATTANGDAVIL
                     HPNGRWEFIDTKKAAEAKVVADQFPENQVCPPGAQGKFLGIGRCIPVGDKDFNRGSLS
                     GKGR"
     gene            complement(2990..5698)
                     /locus_tag="VN084_01015"
     CDS             complement(2990..5698)
                     /locus_tag="VN084_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWT28062.1"
                     /translation="MSLTETKSTCCYCGVGCGVIIQSEDDKITGVRGDPDHPANFGRL
                     CTKGSTLHLTARHDARALYPELRKSREQARQRASWDESLDYVAERFAAIIREHGPDAV
                     AFYISGQLLTEDYYVFNKLAKGLIGTNNVDTNSRLCMSSAVAGYKTTLGADAPPACYE
                     DIDHTACLFIAGSNTAFAHPILYRRIEDAKARNPDLKVIVVDPRRTDTAQAADLHLAI
                     LPGTDVALFNGLLHVMLWEGMLDMAFIREHTEGFEALKNTVREYTPKMVADICGIAEA
                     DILKAAQWFGTGPTLSMYCQGLNQSTSGTDKNAALINLHLATGQIGKPGAGPLSLTGQ
                     PNAMGGREVGGMANLLSGHRDLANPEHRQEVAKLWGVADVPATPGKTAVELFDALNDG
                     TVKAVWIACTNPAQSMPDLNNALTALQKAELVVVQDAYQHTDTTHFADVLLPATSWGE
                     KEGTVTNSERRITHITAAVTAPGESRADWRIATEFAHRLGRLLEDRPNAAKLFPYTQP
                     EQVFNEHRETTRGRDLDITGLSYALLDARGPQQWPFRTGDQAGKARLYSDGHFEKADG
                     KAHFSNTVYRATAEKTDARHPLHLNTGRLRDQWHGMSRTGTVAQLFNHVEEPLIAMNA
                     QDMQRRGIQPGDLVKVSNKRGSLVLRAQASDEMLPAQTFIPMHWGSQFMQGLGVNALM
                     PGAFDPTSKQPELKHAAVKIERLDLPWQMTVMRTCGDLRQLQQLRAMMPYFTYASCGL
                     FGRAPGIMILRAAHTEQPDADLIAQLDTLLGMTDDIPMLHYQDAKRGISKRIMVEGDQ
                     VTGVRLMGEVLATAWLKEVMTQGSFTDTLRRWALAPLSAPPAGQQSRGKVICNCLDVA
                     ENEIIDTIQAGADFVTLQNKLKCGTQCGSCVPELKRLVKLHHKFAA"
     gene            complement(5699..6007)
                     /gene="nirD"
                     /locus_tag="VN084_01020"
     CDS             complement(5699..6007)
                     /gene="nirD"
                     /locus_tag="VN084_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWT28063.1"
                     /translation="MSNWIKIVPLNEIPKLGSRVVRSAKGDIAVFRTDDDRVFALHNQ
                     CPHKGGPLSQGIVYGDKVACPLHGWKISLADGNAEEPDEGHTACFAVKVEDGAVYLEL
                     "
     gene            complement(6021..7715)
                     /locus_tag="VN084_01025"
     CDS             complement(6021..7715)
                     /locus_tag="VN084_01025"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HWT28064.1"
                     /translation="MPMKIAVGQSSLAGPRERNEDYCGVVTPTDEQLSTKGALLMVAD
                     GVSGNAGGLEAAEMTVRSVLSDYYATPDTWEVHAALDKVLAAANRWLLAQAAAHRDLA
                     GMATTLSLVILRGTRYYLAHVGDTRIYRLRQGQLQLLTTDHVWDRPDMRHVLKRAVGL
                     DQHLAVDFADGDLREGDVFALMSDGVWESLGQKSIHEIMGLYDNPQLIADQLTREALA
                     RGGSDNATALVVRVAQPGEETLSELLAGGRDLTPPPRMKAGDTLDGLEVVALLHESRN
                     SLLYKVRNPTSGQHFVLKTLQPGLAADADSCQALLNEEWLAKRVVSRYVVQVLPLTLE
                     KRSRLYYVMSWHDGATLQQRLDSGHHFSNTEVSRIGMEIMRGLGALHRLNIVHRDIKP
                     ANIHLGDDQRLRILDLGVALSAGLGSIAGMQNPGTPSFMAPELFEGTAAGTSSDIYAA
                     GVTLYHLLTRRYPYGEIEPFQHPRFGDPVPPTRYRPDIPHWLENIVLKAVAPRPGARF
                     ETAEEMLLALEHGELKPILAPQRTPLIARARLVKWQWIAMFSLILNFLLIYLLVVS"
     gene            complement(7747..8958)
                     /locus_tag="VN084_01030"
     CDS             complement(7747..8958)
                     /locus_tag="VN084_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HWT28065.1"
                     /translation="MQKSFWQAGHKPTLFSAFLYFDLSFMVWVLLGPLAVQIASDLQL
                     SPAEKGLMVATPVLAGALLRIVMGVLVDHIQPKKAGLLGQVIVIAALATAWLHGIHTF
                     QHALLLGIFLGFAGASFAVALPLASRWYPPEHQGTALGIAGAGNSGTVLAALFAPGLA
                     VAFGWTNVFGLALIPLVIVFIAYSLLAKDSPDTPPAKSLSQYLAIMKDKDAWWFMFFY
                     SVTFGGFVGLASSLTIYFNDLYHLGPVVAGYCTAACVFAGSMVRPIGGYIADRIGGIR
                     TLLMMYGLAGLLLLLMSFGQATLTAALLVTIPAMAVLGMGNGAVFQLVPQRFRKEIGV
                     MTGLVGMAGGIGGFYLAASLGWCKQLTGTYQIGLLIFAALAGLAIIGLSSVKTRWRTT
                     WGSATMTSAKV"
     gene            complement(8989..>9539)
                     /locus_tag="VN084_01035"
     CDS             complement(8989..>9539)
                     /locus_tag="VN084_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HWT28066.1"
                     /translation="GHAYGKSIRTVKTCVGSEFCRFGTQNSTQLGIDIEKAFDHMWAP
                     HKVKFAVSGCPRNCAESGIKDVGIIGVDSGWEIYVGGNGGIKTEVAQFLCKVKTAEEV
                     LEYSGAFIQIYREEARYLDRTVHWIERVGLDYVKKRILEDAEGRKAAYERLLFALQGS
                     IDPWAERATKRDVHTEFDTITV"
BASE COUNT         1715 a         2839 c         3092 g         1893 t
ORIGIN      
        1 aggacacgtt gccctgccag gatcttgggc tgcagtgcga tcgcgatcat gtcgagcaac
       61 tgatgcggtt ccagcatgcg acccatgccg gtctcgccac aggcttgctc gccgctgtca
      121 gggcccagca cgtgcacgcc atcctgcctg agttgggcga tattgcgctg cgtcgccggg
      181 ttctcccaca tttgccggtt catcgctggt gccaccatga gtggacattc acgcgccaag
      241 cacagggtcg agagcagatc gtcggccccg ccatgcacca gcttggccat gaaatcggca
      301 ctcgccgggg caatgaggat agcgtctgcc tcgcgtgata attcgatatg cggcatgccg
      361 ttggcgatac tcgcatccca catgtcggtg aatacgcgct tgccggagag cgcttgcatg
      421 gtcactgggg tgatgaagtg ctgcgcggcc gctgtcatga cgacttggac gtcgatgcct
      481 tgcttgacca gcaggcgcac cagttctgcc gatttgtagg cggcgatgcc acctgtgaca
      541 cctaatagca gctttttaat ggaggggtga gccataatct tggaaaatca tcaatagatt
      601 caattttata tgaaagggga gagcgtggcg attaccgatt ggccagcaag cgagcggccg
      661 cgcgagaaac tgctgcagca cggtgcgggg gtattgtcgg atgctgagtt gctagcgatc
      721 ttcctgcgtg tcggcgtcac cggcaaaagc gcggtcgatc tggcgcgcga cctgctcacg
      781 cgctttggta gcctcaacgg catctttgcc gcaagcccgt cagagatcgg cgcggtacat
      841 ggcatgggcg tcagcaagta cgcgcaactg caggcaatct ttgaaatgag ccggcgcgcc
      901 ctcgcggagc agatgcgtga aagcgacgtg ctaggctcgc cgaagcaggt tcgggattac
      961 ttatgcttgc gcctgggtca gatgaagcgt gaggtgttca tcgtattgtt cctcgacgcc
     1021 cagaaccggg tcgtcgcgca ggaagagatg ttcagcggga ccctcaccca gaccagcgtt
     1081 tatccgcgcg agatcgtcaa gcgtgcgctg catcacaacg cggcatcggt gatcttcgcc
     1141 cataaccatc cctctggcgt tgcccagcaa agtcgcgccg acgaactgct gacggatgcc
     1201 ttgaaacagg ccttggcgct ggtcgatatc cgcacgctcg atcatatcgt catcgccggc
     1261 aacaccagtc tctcgttcgc cgaacagggc ttgctttgac gcgccttcag cgctcgtcgt
     1321 tctgtgattc ggcgagacgc cggatacgtg cttcttcttc cttcttcaca tcattgatga
     1381 cccgcatgac ggtcttcagg tcgaccggtg cgctactttc gcggaaatgg ccagtaagga
     1441 gggtgtctgg cgtgagggcg ccatgcgtgt acagtgacca gatctccttg ccgtattgcg
     1501 aactgcgcag ctccggcgcg aatcggccaa aataatggct caggttgtcg atgtcgcgtt
     1561 ctaacaaggc ctgcgcttgg ttgttgccgg cagcatcgac cgcctgcggc aggtcgatga
     1621 tcaccgggcc ttcgctgcca agcaggatat tgaattccga cagatcgccg tggatgatgc
     1681 cggcgcaaag catgcgtacg acttcggtga tcagcgtcgc gtgatgcttg cgggcttctt
     1741 ccgggctgag ctcgacgtcg ttgagcctgg gtgccgcatg gccgtcaacg tccgtgacca
     1801 gttccatcaa gagcacgccc tcatagaaat tgtagggttg cggtacccga acgcctgctg
     1861 aagccaatcg gtagagtgca tcgacctctg cgctctgcca ggcttgttcc tggacctcgc
     1921 gcccatagcg ggagcccttc tgcatggcac gcgcctgccg gctgttcttg gtcttgcggc
     1981 cctcggtgta atccacgttc tggcggaagc tgcgttggtt ggcttccttg tagaccttgg
     2041 cgcaacggac ttcgtcgcca cagcgcacaa cgtatacggt ggcttccttg ccgctcatga
     2101 gctggcggct gacctcatcg atcaacccgt cctcgatcaa tgcctggagt cgttttgggg
     2161 gtttcatgtc gccatgatag gggttttcag tcggcggcgc gatccggctg gttgcagcaa
     2221 gtcgcctgag aggggctagg cgcagctatc ttcgatgtcc tgataattgg cctgctgaaa
     2281 gcggggtgtg cagagtgcga ggaacaccag gtcagagtcg cccgggttgg tgatgcgttg
     2341 cggacagcca gcggggatga tgacgacatc gccgggcatc acggtctgcg aacctagccc
     2401 tttgacctcg acctgaccat gcccttctag gatcacataa cgctcggcgg tgtcgatcac
     2461 ccggtgccag cgtgtactca ctccgggcag cacgcgtgcc cgggcgatcg atagcccggc
     2521 gtcctcaggt atgttcgaca actccaggat atagcatcgc tctggcgtga agaattcccg
     2581 ttcggggtct gcctgcctga tctgcgcctt catgccggag tcgggtgctc tgggtccgac
     2641 tggcgatcag ccaggagaat cagcggccct tgccgctgag cgagccgcga ttgaaatcct
     2701 tgtcgccgac cgggatgcaa cggccgatgc ccaggaactt gccctgtgcg ccgggcgggc
     2761 agacttgatt ctccggaaac tgatcggcaa cgaccttcgc ttcggccgcc ttcttcgtat
     2821 cgatgaattc ccagcggccg ttcggatgca ggatgacggc gtcgccgttg gcggtggtcg
     2881 cctggatcgg cgcagcgtcg gcaggcgcag gactgtcgtc ggccagtgcg gcctggctga
     2941 caaggatgca gagcatcact agggaaagga acgttttcat cggggcaccc tatgcagcga
     3001 atttatgatg gagtttgact aggcgtttca gctctgggac gcaggaaccg cattgcgtac
     3061 cgcatttgag cttgttctgc agtgtaacaa aatccgcgcc agcctggatg gtgtcgatga
     3121 tctcgttctc cgcgacgtcg aggcaattgc agatgacctt gccgcggctc tgctggccgg
     3181 caggtggcgc gctcagcggg gccagggccc agcgtcgcag tgtgtcggtg aagctgcctt
     3241 gcgtcatgac ttccttgagc caggcggtcg ccagcacttc gcccatcaag cgcacgccgg
     3301 tgacctggtc gccttcgacc atgatgcgtt tgctgatgcc gcgcttggca tcctggtagt
     3361 gcagcatggg gatgtcgtcg gtcatgccga gcagggtatc gagttgcgcg atgaggtcgg
     3421 catctggctg ttcggtatgc gccgcacgca ggatcatgat gcccggcgcg cgaccgaaga
     3481 ggccgcagct ggcataggtg aaatacggca tcatggcgcg caactgctgg agttggcgca
     3541 ggtcgccgca agtgcgcatc accgtcatct gccagggcag gtcgagcctc tcgatcttga
     3601 cggcggcatg tttcagttcg ggctgtttgg aggtcgggtc gaacgcgccg ggcatcagcg
     3661 cattgacccc caggccttgc atgaactgac tgccccagtg catggggatg aaggtctgcg
     3721 ccggcagcat ctcatcggaa gcctgcgcac gcagcaccag gctgccacgc ttgttgctga
     3781 ccttcaccag atcgccgggc tggatgccac ggcgctgcat gtcctgggcg ttcatggcga
     3841 tgagcggttc ttcgacatgg ttgaacaact gggcgacggt tcctgtgcgg ctcatgccat
     3901 gccactggtc gcgtaggcgt ccggtgttga gatgcaatgg atgacgcgca tcggtcttct
     3961 cggcggtggc gcgatagacg gtattggaga aatgcgcctt gccgtcggcc ttctcgaagt
     4021 gaccatcgct gtagagacgt gccttgcccg cctgatcgcc tgtacggaac ggccattgtt
     4081 gtgggccacg cgcatcaagc agggcatagc tgaggccggt gatgtcaaga tcacgaccgc
     4141 gtgtggtctc gcggtgttcg ttgaagacct gttccggctg ggtatagggg aaaagcttgg
     4201 cggcgttggg ccggtcctcg agcaggcggc cgaggcggtg cgcgaactcg gtggcgatac
     4261 gccagtctgc gcggctctcg ccgggcgcgg tgacggccgc ggtgatgtgt gtgatacgac
     4321 gttcggaatt ggtgacggtg ccttctttct cgccccaaga ggtggccggc aacagcacgt
     4381 cggcaaagtg tgttgtgtcg gtatgctgat aggcgtcctg taccacgacc agctcggcct
     4441 tttgcagcgc ggtcagcgca ttattgagat caggcatgga ttgcgccggg ttggtacagg
     4501 cgatccagac tgctttcacc gtgccgtcat tgagcgcgtc gaatagctcc accgcggtct
     4561 tgcccggcgt cgcgggcaca tcggcgacgc cccaaagctt cgcgacttct tgccggtgtt
     4621 cggggttggc gaggtcgcga tggcccgata gcaggttggc catgccgcca acttcacggc
     4681 cgcccatggc attaggctgg ccggtcagcg acagcggccc ggcgcccggc ttgccgatct
     4741 ggccggtggc caggtgcagg ttgatgaggg cggcattctt atcggtgccg ctggtggatt
     4801 gattcagccc ctggcagtac atcgagaggg tggggccggt accgaaccac tgcgccgcct
     4861 tgaggatatc cgcttcagcg ataccgcaga tatcagccac catcttcggc gtgtattcgc
     4921 gcaccgtgtt cttcagtgcc tcgaagcctt cggtgtgctc acggatgaac gccatgtcga
     4981 gcatgccttc ccatagcatc acgtgtagca ggccgttgaa cagcgcgaca tcggtgcccg
     5041 gcaggatggc gaggtgcaga tcagccgcct gcgcggtatc cgtgcggcgc ggatcgacga
     5101 cgatgacctt caggtcgggg ttcctggcct tggcatcctc gatgcggcga tagaggatgg
     5161 gatgggcgaa cgcggtgttg gaaccggcga tgaacaggca cgccgtatgg tcgatgtcct
     5221 cgtagcaagc tggcggtgcg tcggcaccca aggtcgtctt gtaaccagcg acggcggaag
     5281 acatacacag gcgcgagttg gtatcgacgt tgttggtgcc gatgaggccc ttggccagtt
     5341 tgttgaagac gtagtaatcc tcggtcagta attgaccgga gatgtagaac gcgacggcat
     5401 ccggaccgtg ttcccggatg atggcggcga agcgttcggc gacatagtca aggctctcgt
     5461 cccagctggc gcgttgcctc gcttgttcgc gtgacttgcg caattcgggg tacaaggccc
     5521 gggcgtcatg tcgtgctgtc aggtgcagcg tcgaaccctt ggtgcacaga cgaccgaaat
     5581 tggccgggtg atccgggtcg ccgcgtacgc cggtgatctt gtcatcttcg ctctggatga
     5641 tgacgccgca gccgacgccg cagtagcaac aggtggattt ggtttcagtg aggctcaatt
     5701 acagctccag ataaaccgca ccgtcttcca ccttgaccgc gaagcaggcg gtatggcctt
     5761 catccggttc ttcggcgttg ccatcggcga ggctgatctt ccagccgtgc agcgggcagg
     5821 cgaccttatc gccgtagacg atgccttgag acagtggccc gcctttgtgc gggcactggt
     5881 tatgcagcgc gaacacgcgg tcgtcgtcgg tgcgaaacac cgcgatatcg ccttttgcgc
     5941 tacgcacgac gcgcgagccc agcttgggga tctcattgag gggaacgatc ttgatccagt
     6001 tactcatacc ttcatcactt tcaggaaaca accaatagat atatcaacag aaaattcagg
     6061 atcagcgaga acatcgcgat ccattgccac ttgaccaggc gcgcgcgggc gatcagcggg
     6121 gtgcgttgcg gcgcaaggat gggtttcagt tcgccatgtt ccagcgccag cagcatctcc
     6181 tcagcggtct cgaagcgcgc ccccggcctc ggcgcgaccg ccttgaggac gatgttctcg
     6241 agccagtgcg ggatatccgg acggtaacgc gtcggcggca ccggatcgcc gaaacgaggg
     6301 tgctggaacg gctcgatctc gccgtaggga tagcgccgcg tcagcaggtg ataaagcgtg
     6361 acgccagcgg cgtagatgtc gctgctggtg ccggctgccg tgccttcgaa cagctccggc
     6421 gccatgaaac tcggcgtgcc cggattctgc atgccggcga tgctgccgag accggcgctc
     6481 agcgccacgc cgaggtcgag gatacggagg cgctggtcgt cgccaaggtg gatgttcgcc
     6541 ggcttgatgt cgcggtgcac gatattcagg cgatgcagcg cgcccaggcc acgcatgatc
     6601 tccatgccga tacgcgacac ttcggtattg ctgaaatgat ggccgctatc cagccgttgc
     6661 tgcagcgttg cgccgtcgtg ccagctcatg acgtaataca gccggctgcg cttctcaagc
     6721 gtcagcggca gcacctgcac gacatagcgc gagaccacac gcttggcgag ccattcctcg
     6781 ttgagcagcg cctggcagct gtcggcatcg gcggccaacc ccggctgcaa agtcttgagc
     6841 acgaaatgct gcccgctggt ggggttgcgc accttgtaca gcaggctatt gcgggattcg
     6901 tgcagtaacg ccacgacttc caggccgtcg agcgtgtcgc cagctttcat gcgcggcggc
     6961 ggcgtcaggt cgcgtccgcc agccagcagt tcggacaggg tctcctctcc cggttgggcc
     7021 acccgcacca ccagcgcggt cgcgttatcg ctgccgcctc gggcgagtgc ttcgcgggtc
     7081 aactgatcgg cgatgagttg cgggttatcg tacaggccca tgatctcatg gatggatttc
     7141 tggcccaggc tctcccagac gccatccgac atcagcgcga agacgtcgcc ctcgcgcaga
     7201 tcgccatcgg cgaaatccac cgccagatgc tggtcgagac cgacggcgcg tttcagcaca
     7261 tgacgcatgt ccgggcggtc ccacacgtga tcggtggtca acagttgcag ctggccctgg
     7321 cgcaggcgat agatgcgcgt gtcgccgacg tgggccaggt aatagcgggt gccgcgcagg
     7381 atgacgagcg agagcgtcgt ggccatgccg gcgagatcgc gatgtgcagc ggcctgcgct
     7441 agtagccagc ggttggcggc agccagcacc ttgtcgagcg cggcatgtac ctcccacgtg
     7501 tccggcgtcg cgtagtagtc ggacagcacg ctgcgcacgg tcatctcggc ggcttccagc
     7561 ccgccggcgt tgccgctgac gccgtccgcc accatcagca gcgcgccttt ggtgctgagc
     7621 tgctcatcgg tgggcgtgac gacgccacag taatcctcgt tgcgctcgcg cggtcccgcc
     7681 aggctactct gcccgacggc gatcttcatc ggcatgacat gactcttgaa ctcgaacggt
     7741 ttcagtttaa acctttgccg aggtcatggt ggccgagccc caggtcgtgc gccagcgtgt
     7801 cttgacgctg ctgagaccga tgatggcgag gcccgccagc gctgcgaaga tcaggagacc
     7861 gatctgatag gtgcccgtca gttgcttgca ccaacccaga cttgccgcca gatagaaacc
     7921 accgatgccg ccagccatgc cgacgagccc ggtcatgacg ccgatctcct tgcggaagcg
     7981 ctggggcacg agctggaaca cggcgccgtt gcccatgccg agcacggcca tggccgggat
     8041 ggtcaccagc agcgcagcgg tcagcgtggc ctggccgaaa ctcatcaata gcaagagcag
     8101 gcctgccagg ccatacatca tgagcagcgt gcggataccg ccgatgcggt cggcgatata
     8161 gccgccgatg gggcgcacca tggagccggc gaagacgcag gcggcggtgc agtaacccgc
     8221 gaccacgggg cccagatgat agagatcgtt gaagtagatc gtcagcgagg aggccaggcc
     8281 gacgaagccg ccgaaggtca cgctgtagaa gaacatgaac caccaggcat ccttgtcctt
     8341 catgatcgcc agatactggc taagcgactt ggcgggtggg gtgtccgggc tatccttggc
     8401 gagcaggctg taggcgatga aaacgatcac cagcgggatc agggcgaggc cgaagacgtt
     8461 ggtccagccg aacgcgacgg ccagaccggg cgcgaacagc gccgccagca cagtccctga
     8521 attaccggca ccggcgatgc ccagcgcagt gccctggtgt tccggcggat accagcgtga
     8581 ggccaggggc agggcgacgg cgaacgatgc gccggcgaag ccgaggaaga tgcccagcag
     8641 caaggcgtgc tggaaggtat ggatgccatg cagccaggct gtggccagcg ccgcgatgac
     8701 gatcacctgc cccagcagcc cggctttctt cggctggata tgatcgacca gtacgcccat
     8761 gacgatacgc agcagggccc cggcgagcac cggggtcgcg accatcaggc ccttttcagc
     8821 cgggctgagc tgcaggtcgc tggcgatctg cacggcgagc gggccgagca gtacccacac
     8881 catgaaactc aggtcgaagt ataggaaggc ggagaacagg gtaggcttgt ggccggcctg
     8941 ccagaaactc ttttgcatga aatactcctc agggatgctg gttatcggtt acaccgtgat
     9001 ggtgtcgaat tcggtgtgca cgtcgcgctt ggtggcacgc tccgcccacg gatcgatcga
     9061 accctgcaag gcgaacagca ggcgctcgta ggcggcctta cggccttcgg catcttccag
     9121 gatgcgcttc ttgacatagt cgaggccgac acgctcgatc cagtgcacgg tacggtcgag
     9181 atagcgcgcc tcttcacggt agatctggat gaaggcgccg gaatattcca atacctcttc
     9241 tgccgtcttc accttgcaca ggaactgcgc gacctcggtc ttgatgccgc cgttgccgcc
     9301 gacatagatc tcccagccgg aatcgacgcc gatgatgccg acgtccttga tgccggattc
     9361 ggcgcagttg cgcgggcagc cagagacggc gaacttgacc ttgtgcggcg cccacatgtg
     9421 atcgaacgct ttttcgatat cgatgccaag ctgcgtcgaa ttctgtgtgc cgaagcggca
     9481 gaactcagag ccgacacagg tcttcaccgt gcggatgctc ttgccgtagg cgtggcccg
//
LOCUS       DATMTW010000052         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_507901, whole genome shotgun sequence.
ACCESSION   DATMTW010000052 DATMTW010000000
VERSION     DATMTW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507901"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VN084_01040"
     CDS             <1..188
                     /locus_tag="VN084_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWT28067.1"
                     /translation="ASKFGGDGSAIYTRENRIVKSSKGLLAQIRNCNTMLGLKWFEDE
                     ELHVASYLNKTYYKFEQ"
     gene            178..1041
                     /gene="rsgA"
                     /locus_tag="VN084_01045"
     CDS             178..1041
                     /gene="rsgA"
                     /locus_tag="VN084_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWT28068.1"
                     /translation="MNSKGLVVGAYGKRYDVELSDHRVISCVTRGKKTDLACGDHVEI
                     QSTADGEGVVERYLPRTSLLYRSNAYRTKILAANVTQAIIVLATQPSFYEELLNRCVV
                     AAEAAGIKPLILLNKCDLAAYDETMQRLAYYQHLGYSVQPLSAKRDIEPLKPHLAGQV
                     SVLVGQSGMGKSTIVNALLPHLALRTQEVSTVLDSGKHTTTATHLYHLDATSDLIDSP
                     GLQEFGLYHLSIEQLEHAFIEFRPYLGKCRFNDCRHHKEPDCAILAAVGSGTISPVRL
                     QCYQGLLRELG"
     gene            1055..1978
                     /locus_tag="VN084_01050"
     CDS             1055..1978
                     /locus_tag="VN084_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HWT28069.1"
                     /translation="MTTAFINHPDTLLHVMDGRHPESPARITAIRDRLASDGLLAKLA
                     RYEAPAADDIQLRRVHTQAYIDRIRALSPRAGLVQLDPDTAMGPMSLSACLHASGAAV
                     MATDLVMRGEASNAFCCTRPPGHHAGREYAAGFCIFNHVAVGAAHALAQYGLERVAIV
                     DFDVHHGDGTEDIFKDRSQVMLCSTFQHPFYPHKGADSRTERMVNVPLPAGTGSEAFR
                     AAVEKEFAPALARFKPQMVFVSAGFDAHADDPLAQLQLTTDDYRWVTRFIRQVADRHA
                     EGRIVSILEGGYRLSDLADSAAAHVAALAAG"
     gene            2007..2447
                     /gene="queD"
                     /locus_tag="VN084_01055"
     CDS             2007..2447
                     /gene="queD"
                     /locus_tag="VN084_01055"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWT28070.1"
                     /translation="MQITTRLEFDAGHRIPSHKSQCRNLHGHRYAMEITLSGDIIRQE
                     GVSENGMVMDFSDVKTIARRLVVDVWDHAFLVYRGDQVVMDFLATLPDHKTVIMDTVP
                     TAENMAAEAFRLLAAGYHDTYGNHLKLERVRLYETPNSWADALG"
     gene            complement(2465..3382)
                     /gene="rfbD"
                     /locus_tag="VN084_01060"
     CDS             complement(2465..3382)
                     /gene="rfbD"
                     /locus_tag="VN084_01060"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWT28071.1"
                     /translation="MPAFGTILLTGIHGQVGSVLQTDLGALGDVVGVSRAQLDLADAD
                     AIRRTVRELRPGLIVNPAAYTAVDKAEAEPELAFVINAEAPRILAEEAARLAIPLVHF
                     STDYVFDGRKTEAYLETDNPNPLGVYGKSKLAGEEAIRASGAQHLILRTSWVYGAYGN
                     NFMKTMIRLATQRDSLRVVADQFGAPTSSHTIAAATCRALTDWRATASGTYHLTNRGR
                     TSWHGFAQAILDHYQRLQASRGWPPLQVAPEAVVAIGTADYPTAATRPANSCLDCSKF
                     VAAFGNGLPEWREALEEEMQALQMELPRA"
     gene            complement(3382..>3582)
                     /gene="rfbC"
                     /locus_tag="VN084_01065"
     CDS             complement(3382..>3582)
                     /gene="rfbC"
                     /locus_tag="VN084_01065"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWT28072.1"
                     /translation="FAHGFLVLSESAEFLYKTTDFYAPEHERCLRWDDPALAIEWPLQ
                     GAPILSAKDAQGQTLDMAELFD"
BASE COUNT          715 a         1153 c         1034 g          680 t
ORIGIN      
        1 atgccagcaa gttcggcggt gacggttcgg ccatctacac gcgtgaaaac cgcatcgtca
       61 aaagttccaa gggtctgctg gcgcagatcc gtaactgcaa caccatgctg ggcctgaagt
      121 ggttcgaaga cgaagaactg catgtcgcca gctacctcaa caaaacctat tacaagtttg
      181 aacagtaaag ggctggtggt cggcgcctac ggcaagcgct acgacgtcga actctccgac
      241 caccgtgtca tcagctgcgt cacccgcggc aagaagacag acctcgcatg cggtgatcat
      301 gtcgagatcc agtcgaccgc cgacggcgaa ggcgtcgtag aacgctatct gccccgcacc
      361 tccctgctat accgcagcaa cgcctaccgc accaagatct tggctgccaa cgtcacgcag
      421 gccatcatcg tgctggccac ccagccaagc ttctacgaag aattgctcaa tcgctgtgtg
      481 gtcgcggcag aggctgccgg tatcaagcct ttgatcctgc tcaacaagtg cgacctggcg
      541 gcctatgacg agaccatgca gcgcctcgcg tattaccagc atctgggtta cagcgtgcag
      601 ccgttatcgg caaagcgcga catcgagccc ttgaagccgc acctggctgg gcaggtcagc
      661 gtgctggtcg gtcagtccgg catggggaaa tccaccatcg tcaacgccct gctgccgcat
      721 ctggccttgc gcacgcagga ggtgtcgacc gtgctggata gcggcaagca caccacgacc
      781 gccacccatc tctaccatct ggacgcgacc agcgacctca tcgattcacc tggactgcag
      841 gaattcggcc tttaccatct gagcatcgag caactcgaac acgcgttcat cgaattccgg
      901 ccatatctcg gcaaatgccg attcaacgac tgccgccatc acaaggaacc ggattgcgcg
      961 atcctcgccg ctgtcggaag cggcacgatc tcccctgtgc gcctgcagtg ctaccagggg
     1021 cttctgcgcg agctcggttg aacaggattg cgcgatgacg accgccttca tcaaccaccc
     1081 cgacaccttg ctgcatgtca tggatggccg tcaccctgaa tcccccgcgc gcatcaccgc
     1141 tatccgcgat cgccttgcca gtgatgggct gctggcgaaa ctggcgagat atgaggcacc
     1201 ggcggctgac gacatccagc tgcggcgcgt gcatacgcag gcatacatcg atcgcatccg
     1261 cgcgctatcg cccagagcgg gtctcgtgca gctcgatcca gataccgcca tgggcccgat
     1321 gagtctctct gcctgcctgc acgccagcgg cgcggccgtc atggccaccg atctggtcat
     1381 gcgcggtgaa gcaagcaatg ctttctgttg cacccgcccg cctggccatc atgccggccg
     1441 tgagtatgcg gccgggtttt gcatcttcaa ccacgtcgcg gtaggcgcgg ctcacgccct
     1501 tgcgcaatat ggactggaac gggtcgctat cgtcgatttc gatgtccatc acggggacgg
     1561 cacggaagat atcttcaagg accggtcaca ggtcatgctc tgctctacct tccagcatcc
     1621 gttctacccc cacaagggcg cagatagccg aaccgagcgc atggtcaatg ttcccctgcc
     1681 ggcagggacc ggcagtgagg cttttcgcgc tgccgtagag aaggaattcg cgccggcact
     1741 ggcgcggttc aaaccacaga tggtgttcgt ctcggccggc ttcgacgcac acgccgacga
     1801 cccgctggcg caactacagc tgacgaccga cgattatcgc tgggtcacga ggttcatacg
     1861 gcaagtcgcc gaccggcatg ccgagggccg tatcgtatcg atcctcgaag gaggttatcg
     1921 cctgagcgat ctcgcggata gcgctgccgc ccacgttgcg gctttggcgg ccggctaagc
     1981 gccgcgcatg ctgctataat cgcgccatgc aaatcaccac ccgcctggaa ttcgatgcag
     2041 gacatcgcat tccctcccac aagagccagt gccgcaactt gcacggccac cgctacgcca
     2101 tggaaatcac cctctccggc gacatcatcc gacaggaagg cgtctcagag aatggcatgg
     2161 tgatggattt ttcggatgtg aagacgatcg cgcggcgctt ggtcgttgat gtctgggatc
     2221 atgctttcct cgtctatcgc ggtgaccaag tggtgatgga ttttctcgcc accctgcctg
     2281 accacaagac tgtgatcatg gacacggtgc cgaccgcgga gaatatggcg gcagaggcgt
     2341 tcagattact agcggctggt taccatgaca cgtatggcaa tcatctcaag ctggagcgcg
     2401 tgcgcctcta cgaaaccccc aacagctggg cggacgctct gggctgagcc caaacagaat
     2461 cgctttaggc cctcggcagc tccatctgca gggcctgcat ctcctcttcc aaggcttctc
     2521 gccactctgg cagaccattc ccgaaagccg ctacgaactt gctgcaatcc aggcaggaat
     2581 tcgccggcct cgtcgctgca gtcggataat ccgccgtgcc gatcgcgacg acagcttctg
     2641 gtgccacctg caaaggcggc catccacggc tcgcctgcag gcgttggtaa tgatccagga
     2701 tcgcttgtgc gaaaccatgc caggaagtac ggccgcgatt ggtcaggtga taggttccgg
     2761 aggcagtcgc ccgccagtcg gtgagggccc ggcaagttgc tgccgcgatg gtgtggctgc
     2821 tggtgggcgc cccgaactgg tcagccacca cccgcaggct gtcccgctgc gtggccagcc
     2881 tgatcatggt cttcatgaaa ttgttgccat atgcgccgta gacccagctg gtgcgcagga
     2941 tcaggtgctg ggcgccgctc gcacggatcg cctcctcgcc cgccagcttg ctcttgccgt
     3001 agacccccag cggattcggg ttgtcagtct cgagataagc ctcggtcttg cggccatcaa
     3061 agacgtaatc ggtagagaaa tgaaccagcg ggatcgccag ccttgcagct tcctcggcaa
     3121 ggatgcgtgg cgcctctgcg ttgatgacga acgccagttc tggctctgcc tcggccttgt
     3181 ccacggccgt gtaggctgca ggattgacga tcaggcccgg acgcaattca cgtaccgtgc
     3241 ggcggatggc atcagcatcg gcaagatcca gttgcgcgcg tgagacgcca acaacatctc
     3301 ccagcgcccc cagatccgtc tgcaaaaccg agccgacctg gccatggatg ccggtcagca
     3361 agatagtccc gaatgccggc atcagtcgaa cagctcggcc atgtccagcg tctggccttg
     3421 cgcatccttg gcggacaaga tcggcgcccc ttgcagaggc cattcgatcg ccagcgctgg
     3481 atcatcccag cgcaggcaac gctcatgttc cggcgcgtag aaatctgtcg tcttgtagag
     3541 gaattctgcg gactcggaca ataccaggaa gccgtgggca aa
//
LOCUS       DATMTW010000053         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_306469, whole genome shotgun sequence.
ACCESSION   DATMTW010000053 DATMTW010000000
VERSION     DATMTW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306469"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VN084_01070"
     CDS             complement(<1..1184)
                     /locus_tag="VN084_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWT28073.1"
                     /translation="MIVTFPNSKYALHQPFPPAGDQPQAIEKLVNGIEQGMKFQTLLG
                     VTGSGKTYTMANVIARTGRPAIVMAPNKTLAAQLYSEFREFFPNNAVEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINDHIEQMRLSATKAILEREDCVVVATVSAIYGIGDPGEYH
                     GMVLHVHQGMKVSQREIVAKLIAMQYDRNDFDFSRGAFRVRGDVIDVFPSENSETAVR
                     ISLFDDEIEAITLFDPLTGQIFNKIHNFRIFPSSHYVTAREATIRAMETIKQELRERV
                     DFFIKNQKLVEAQRIEQRTRFDLEMLNEIGFCKGIENYSRHLTGRKPGEPPPTLIDYL
                     PANALMIIDESHVTVPQVGGMYKGDRARKENLVDYGFRLPSAMDNRPLRFEEFETVMR
                     QCV"
     gene            1318..2334
                     /locus_tag="VN084_01075"
     CDS             1318..2334
                     /locus_tag="VN084_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWT28074.1"
                     /translation="MRRLLLLLIALSLAPTAYAYDRAKLFKAFLSVVMVRGYNPDGSM
                     AYGSGVVVAPNQVATNCHIFRKTKQPWISRGEDSYNITSVKADRYRDLCLVTTTNMPF
                     EAVEMLKHPDLQKGQEIVAIGHSSGVPAPLTSLGTLKSVYPYKGGNILRSNARFAMGA
                     SGSGMFDSEGRLLGINTFKTPGREAYFYALPISWLDDIEKLPTETTFPIDGMAFWEEE
                     DAKKPYFMQIALPEIDEDWPKLAQVAEQWTKEEPDSTEAWYELGNAQEHLDRDSEAEA
                     AYRKSIELSATNTDALFRLGVMASKKGDTAEVKSINTSLLALNKDIAEEFNKAITCKD
                     QCKN"
     gene            2436..3629
                     /locus_tag="VN084_01080"
     CDS             2436..3629
                     /locus_tag="VN084_01080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWT28075.1"
                     /translation="MELSQRVQSIKESPTLAVTARAARLKAEGKDIIGLGAGEPDFDT
                     PQHIKDAAKKAIDAGFTKYTPVAGIPGLKKAIVHKFKQENGFDYTEKEVIVGVGGKQC
                     IFNLALAVLNPGDEVIVPAPYWVSYADIAMVAGAKPVIIECGIEQGFKLLPAQLEAAI
                     TPKTKLFMINSPSNPTGAVYTLAELQALGEVLKRNPHVLVATDDMYEHVNLSGERFHN
                     ILNATPELKDRCIVLNGVSKAYSMTGWRIGYAAGPAHIIKAMEILQSQSTSNPTSISQ
                     VAAQAALEGPQSCITPMVEAFRERHAYVVDRFNAMPGLKCIKAGGAFYAFPDARIAIS
                     NLHKAGKIHEATDMALSEYLLEKVGVAVVPGSAFGAEGYFRISFATSMENLRNALDRI
                     EQALS"
     gene            complement(3698..>3910)
                     /locus_tag="VN084_01085"
     CDS             complement(3698..>3910)
                     /locus_tag="VN084_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWT28076.1"
                     /translation="DLKKGVSLATIPLQVSGTVSKPSVFPTKAAMAGAVAGTAVLGPG
                     VGTTLGVKAGSAVEKIKGLFGGDDKD"
BASE COUNT          883 a         1146 c         1054 g          827 t
ORIGIN      
        1 acgcattgcc gcatgacagt ctcgaactcc tcgaagcgca aaggacggtt atccatggcc
       61 gaaggcaggc ggaatccgta atcgaccagg ttctccttgc gcgcccggtc gcctttatac
      121 atgccgccga cttgcggcac ggtgacatgg ctttcgtcaa tgatcatcag cgcattggcc
      181 ggcagatagt cgatcagggt cggtggcggc tcgcccggct tacgtccggt gaggtggcga
      241 ctgtagttct cgatgccctt gcagaagccg atctcgttca gcatctcgag gtcgaatcgc
      301 gtgcgctgct cgatgcgctg cgcctcgacg agcttctggt tcttgatgaa gaaatccaca
      361 cgctcccgca gctcctgctt gatggtctcc atggcacgga tcgtcgcttc gcgcgcagtg
      421 acgtaatgac tggaagggaa gatgcggaaa ttgtggatct tgttgaagat ctgcccggtc
      481 aacgggtcga acagcgtgat cgcttcgatc tcatcgtcga acaggctgat gcgcaccgcc
      541 gtctcgctgt tttcggacgg gaacacatca atgacgtcac cacgcacgcg gaaagcgcca
      601 cgcgaaaagt caaaatcgtt gcgatcgtat tgcatggcga tgagcttggc gacgatctcg
      661 cgctggctga ccttcatgcc ttggtgcaca tgcagcacca tgccatggta ttcgccggga
      721 tcgccgatac cgtagatcgc ggagaccgtc gccaccacca cgcagtcttc gcgctcaagg
      781 atggccttgg tcgccgaaag ccgcatctgc tcgatgtggt cgttgatgct cgagtccttt
      841 tcaatgaaaa ggtcacgcga agggacatag gcctcaggtt gatagtaatc gtaatacgag
      901 acgaaatact ccaccgcgtt attggggaag aactcacgga attcagaata gagctgcgcc
      961 gccagcgtct tgttcggagc catgacgata gccgggcgcc cagtgcgggc gatgacattg
     1021 gccatggtgt aggtcttgcc ggagccggtg accccgagca aggtctggaa cttcatgccc
     1081 tgctcgatgc cgttgaccag cttctcgatc gcctgcggct ggtcgccagc cggcgggaaa
     1141 ggttgatgca gcgcgtattt gctattgggg aatgtgacga tcacaagctt cactttcaaa
     1201 acagacgctt attctagcag gcatgccgaa ctggctgccg agaactttaa ttacccacac
     1261 tgggtcatca ttaggtattt caagactcat catgacaata acgatctggg gaaacacatg
     1321 cgtcgacttc ttctattact cattgcactc agcctggcac ccacggctta cgcgtatgat
     1381 cgagccaagc tgttcaaggc ctttctgtcc gtcgtcatgg ttcgcggcta taacccggac
     1441 ggtagcatgg cctacggctc cggcgttgtg gttgcaccca accaggtcgc taccaactgt
     1501 cacatcttcc gcaagaccaa gcaaccttgg atctcgcgtg gcgaagatag ctacaacatc
     1561 acttcggtca aggcagaccg ctatcgggac ctgtgtctcg tcaccacgac caacatgccg
     1621 ttcgaggctg tcgagatgtt gaaacatccg gacctgcaga aaggccagga gatcgtcgct
     1681 atcggccact ccagtggcgt accagcgccg ctgacttcac tcgggacctt gaaatccgtc
     1741 tacccataca agggcggcaa catcctgcgc agtaacgccc gcttcgcaat gggtgccagc
     1801 ggcagcggca tgttcgactc cgaaggccgt ttgctgggca tcaatacatt caagacccca
     1861 ggacgcgagg cttacttcta cgccttgccg atctcatggc tggacgatat cgagaagtta
     1921 ccgacggaaa ccactttccc gatcgacggt atggcattct gggaagaaga agatgccaag
     1981 aagccctact tcatgcagat cgccctgccc gagatcgatg aagactggcc caagctggct
     2041 caagtcgccg aacaatggac caaggaagaa cctgatagca ccgaagcctg gtatgaactt
     2101 ggcaacgcgc aggagcatct ggaccgcgac agtgaggcag aagctgccta tcgtaaatcg
     2161 atcgagctct ctgcgaccaa taccgacgcg cttttccggt tgggtgtgat ggcttccaag
     2221 aagggtgaca ccgcagaggt gaagtcaatc aataccagcc tgctggcgtt gaacaaggac
     2281 atcgccgaag agttcaacaa ggccattact tgtaaggatc aatgtaaaaa ctaaaattta
     2341 catttctgtt acgcgctgca gattccgctg tataggtttt gtgcgtaaaa ttagggtttt
     2401 acttggctca accctactca gcaaggactt ttgtcttgga actctcgcag cgcgttcaat
     2461 ccatcaagga atccccaact cttgccgtga ccgcccgtgc ggccagactc aaagccgaag
     2521 gtaaagacat catcggccta ggtgccggag aaccggattt cgacaccccg caacatatca
     2581 aagacgctgc gaaaaaggcc atcgatgccg gcttcaccaa atacacccct gttgccggta
     2641 ttccagggct gaaaaaagcc atcgtacata aattcaagca agagaacggt ttcgactata
     2701 ccgaaaagga agtcatcgtc ggcgtgggcg gcaagcagtg catcttcaac ctggctctag
     2761 ccgtgctgaa tccgggcgat gaagtcatcg tgcccgcgcc ctactgggtc tcttacgccg
     2821 atatcgccat ggtggcaggg gcgaaacccg tcatcatcga atgtggtatc gaacagggct
     2881 tcaagttgct gccggcacag ttggaagccg cgatcacgcc caagaccaag ctgttcatga
     2941 tcaactcgcc ctcaaacccg acgggcgccg tctacacgct ggctgaactg caggccttgg
     3001 gcgaggtgct gaagcgcaac ccccatgtgc tagtcgctac agatgacatg tacgagcacg
     3061 tcaacctcag cggtgaacga ttccataaca tcctcaacgc cacaccggaa ctcaaggatc
     3121 gttgcatcgt actgaacggc gtatccaagg cgtattccat gaccggctgg cgcatcggct
     3181 acgccgcagg cccagcgcat atcatcaagg ccatggagat cctgcaatcg caatccactt
     3241 ccaacccgac ctcgatctcg caggtcgccg cacaggctgc actggaaggc ccacaaagct
     3301 gcatcacgcc gatggtcgaa gccttccgcg agcgtcatgc ttatgtggtc gatcgtttca
     3361 atgccatgcc cggtcttaaa tgcatcaagg ctggcggcgc gttctatgcc tttcctgatg
     3421 cccgtatcgc catttccaac ttgcacaagg ctggcaagat ccatgaggca acagatatgg
     3481 cgctatccga atacctgctg gaaaaagtcg gcgtcgcggt cgttccgggt tcggctttcg
     3541 gtgccgaagg ctatttccga atctcgttcg cgacttcgat ggaaaatctg cggaatgcac
     3601 tcgaccgtat cgagcaggcg ctgagctagt cacatcctgt tagccgcaaa aaaggccttc
     3661 tcatgaaggc ctttttgttt gcgacgtcca ccctgcctca gtccttgtca tcgccgccga
     3721 acaggccttt gatcttctcg acggcagaac ctgccttgac cccgagcgtc gtgccgacgc
     3781 ccgggcccag cacagccgtg cccgcgactg cgccagccat tgcggccttg gtcgggaaca
     3841 cacttggctt gctcaccgtc ccggagacct gcagtgggat cgtcgcaagg ctcacccctt
     3901 tcttcaaatc
//
LOCUS       DATMTW010000054         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_4625, whole genome shotgun sequence.
ACCESSION   DATMTW010000054 DATMTW010000000
VERSION     DATMTW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4625"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VN084_01090"
     CDS             complement(<1..836)
                     /locus_tag="VN084_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HWT28077.1"
                     /translation="MASGLHQQIADFQQWRDQLSQTIGEYRQWLEDTGSSDSLQDLRL
                     FDMMETLRKDHLVLAFVAEFSRGKTETINALFFADYNQRLLPSEAGRTTMCPTEIFWD
                     EREEPCIRLLPIETRKRDDSLAYLKSNPNEWTTLRLDIHSAEAMKESMHSVIQQKPVS
                     LEEARSLGLWNDQDVSMVQSIKARGVVDVPVWRHAMINFPHPLLKSGLVILDTPGLNT
                     LGSEPELTLSIIPNAHAVIFLLATDTGVTRSDMQIWTDFIRTRASRKLALLNKIDILW
                     DE"
     gene            complement(956..1531)
                     /locus_tag="VN084_01095"
     CDS             complement(956..1531)
                     /locus_tag="VN084_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWT28078.1"
                     /translation="MIANALNFLLYTILGLFTLVVLLRFYLQLTGAPFQNPASQAIVA
                     LTNFAVRPLRRIIPGWRGIDFSTLLLAFATQVLLQLGTLWLRDFPLLVADNAVWSAVL
                     GLALIGLLKLSVYIFLYAVLLQAILSWVNPYTPISGVLEMLTRPLMRPLRKRIPLAGG
                     VDLSPLVFFIIAELVLIALLAPLEQQLLMFF"
     gene            complement(1542..2351)
                     /gene="proC"
                     /locus_tag="VN084_01100"
     CDS             complement(1542..2351)
                     /gene="proC"
                     /locus_tag="VN084_01100"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWT28079.1"
                     /translation="MKISFIGGGNMARALISGMLRQGFDAAAISVVDPDADKRASLQR
                     DFGVTTTAQLPSVSAADVIVLAVKPQQLRDLSIFLGSLLQHQLVISIAAGIRCNDLAR
                     WLGGYAAIVRVMPNTPAQVQRGLSALFAMPAVSATQRAQAEQVLQAVGKIMWLDAEAK
                     MDAVTAISGSGPAYVFYFMEALQQAAVELGFTEDEARMLTLQTFSGASALADASAESA
                     AVLRQQVTSKGGTTEQALLSMEDAAVKAAIVRAAHAAAERSRTLGDQLGRD"
BASE COUNT          482 a          674 c          799 g          474 t
ORIGIN      
        1 atctcatccc aaaggatgtc gatcttgttg agcaaagcca gtttgcgact ggcgcgcgtg
       61 cggatgaaat cagtccatat ctgcatgtcg gagcgggtga cgccagtatc ggtggcgagc
      121 aggaagatca cggcatgcgc gttgggaatg atgctcaggg tcaattccgg ttcgctaccc
      181 aatgtgttga ggccgggcgt gtctaggatg accagtccgc tcttcagcag cggatgcggg
      241 aagttgatca tggcgtggcg ccagacgggc acatcgacca cgccacgggc cttgatggat
      301 tgcaccatcg agacatcctg gtcgttccat aacccgagac tgcgagcctc ttccagcgaa
      361 accggcttct gctggatcac ggaatgcatg gattccttca tcgcctcggc tgagtggatg
      421 tcgaggcgca gggtggtcca ctcgttggga ttgctcttga ggtaggccag cgagtcgtcg
      481 cgtttgcgtg tctcgatcgg cagcagcctg atgcagggtt cttcgcgttc gtcccagaag
      541 atctcggttg gacacatggt cgtgcggccg gcttcggaag gcagcaggcg ctggttgtaa
      601 tcggcgaaga aaagggcgtt gatggtttcg gtcttgccgc gggagaactc cgcgacgaag
      661 gccagtacca gatggtcctt gcgtaaggtc tccatcatgt cgaagaggcg caggtcctgc
      721 agtgaatcag agctgccggt atcttccagc cattggcgat actcgccgat ggtctgcgac
      781 agctgatccc gccactgctg gaaatcggcg atctgctgat gaagtccgct cgccatgacg
      841 gttccgctag ccgttctgag acacaggtgg ttcggtcgcc ttattcatct tgtccccgtg
      901 ataaccgatt gatgttgttt gcagatatta cggcatgcaa agcgcgtgac gtcaatcaga
      961 agaacatgag tagctgttgt tcgagcggtg ccagcaaggc gatgaggaca agctcagcga
     1021 tgatgaagaa caccagcggg ctgagatcga cgccgccagc aagcgggatg cgtttgcgca
     1081 aggggcgcat cagtgggcgc gtcaacatct ccagcacgcc actgatcggc gtgtagggat
     1141 tgacccagct gaggatcgct tgcagcagca cggcgtacag gaagatatat acgctgagct
     1201 tgagcaggcc gatcaacgcc aggcccagta cggcgctcca tacggcgttg tctgccacga
     1261 gcaaggggaa atcgcgcagc cagagcgtac ccagctgtag caagacctgg gtggcgaaag
     1321 ccagtagcag ggtcgagaaa tcgatgccgc gccagcctgg aatgatccgc cgcaggggcc
     1381 gcactgcgaa gttagtgagc gcgacgatgg cttgtgaggc gggattctgg aacggagcac
     1441 cagtcagctg caggtaaaag cgcagcagca cgaccagcgt gaacaagccc aggatggtat
     1501 agagcaggaa attcaaagca ttggcgatca tgcgcgtccc cctagtcccg gcccagctgg
     1561 tcgcccagcg tcctggagcg ctcagcggcg gcatgggcgg cgcggacgat ggccgctttc
     1621 acggcagcgt cttccatgct gagcaaggct tgttcagtgg tgccgccctt ggaggtcacc
     1681 tgctggcgca acactgcagc agactctgcg ctggcatcgg cgagcgcact ggccccgctg
     1741 aaggtctgca gtgtcagcat ccgggcttcg tcctcggtga atccgagttc cacggcggct
     1801 tgctgcaggg cctccatgaa gtagaagaca taggcgggcc cactgccgga gatggccgtt
     1861 acggcatcca tcttcgcttc ggcgtcgagc cacatgatct tgcccacggc ctgcaggact
     1921 tgttccgctt gcgcgcgttg tgtcgcgctg acggctggca tggcaaagag cgccgacagc
     1981 cctcgctgga cctgcgccgg ggtattcggc atgacgcgca cgatggcggc ataaccgccc
     2041 agccagcgtg ccaggtcgtt gcagcggatg ccagcggcga tggagatgac caactgatgc
     2101 tgcagcaggc tcccgaggaa gatcgacaga tcgcgcaatt gctggggctt gacggcgagg
     2161 acgatgacat cggcagcgct gacgctgggc aattgtgcag tcgttgtgac gccgaagtcc
     2221 cgctgtagcg atgcacgctt gtcggcatcg gggtcgacga cgctgatggc tgcggcatcg
     2281 aacccttgcc gaagcatgcc gctgatgagc gcgcgtgcca tgttgccgcc gccgatgaaa
     2341 ctgattttca tatgcttcct gtcgatcatg attgcgttat acgtgcggcg cgcgggccga
     2401 aaatggcaga gccgatgcgg atgatggtg
//
LOCUS       DATMTW010000055         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_575226, whole genome shotgun sequence.
ACCESSION   DATMTW010000055 DATMTW010000000
VERSION     DATMTW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575226"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1443
                     /gene="gshA"
                     /locus_tag="VN084_01105"
     CDS             166..1443
                     /gene="gshA"
                     /locus_tag="VN084_01105"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWT28080.1"
                     /translation="MVPHLTTALNGPLLELEKRMLAAMPQIEHWFRTQWQEHTVPFYA
                     SVDLRNAGFKLAPVDTNLFPGGFNNLNPDFLPLCVQAAMSAVEKICPEARRLLVIPEN
                     HTRNTYYLRNVAALTNILRQAGMEVRIGSISPEITTTTTLDLQDGQTLQLEPVQRKGN
                     RLVLDGFDSCAILLNNDLSGGVPDVLQRLEQDLIPPLHAGWATRRKSQHFSAYNQVVD
                     EFAQLLEIDPWLLNPYFDTCSEIDFHARTGEACLSSRVEKLLEKIRIKYAEYGVKQDP
                     FVIVKADAGTYGMGIMTVKSPDDVRDLNRKQRNKMAVVKEGLEVSEVIIQEGVYTFEN
                     LNDAVAEPVIYMIDHYVVGGFYRVHTGRAVDENLNAPGMHFVPLAFERSCSLPDHSAA
                     PDAPPNRFYAYGVVARLALLAAAIELETHAPEQ"
     gene            1449..2393
                     /gene="gshB"
                     /locus_tag="VN084_01110"
     CDS             1449..2393
                     /gene="gshB"
                     /locus_tag="VN084_01110"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWT28081.1"
                     /translation="MRLLFILDPLDQLKTYKDTSIAIMRAAAARGHTLFTAMQGDILL
                     RHERVVVRASAFRFAEGASWYEGDAAFETVPADFDAVLMRKDPPFDNEYLYSTYLLEL
                     ATQQGAHVYNAPESIRGWNEKLSIARFAEFAPPFLVTANNTLIRDFLASHGDIIVKPL
                     DGMGGSGVFRLRTDDANIGVILETVTHFGERTVMAQRYVPEIKVGDKRILMIDGQPLP
                     YALARVPQGGETRGNLAAGGKGVAQPLSPRDLEIANRIGPVLREHGLFLVGLDVIGDY
                     LTEVNVTSPTGMVEIAAQTDCDPASLFMDALESACAAS"
     gene            2378..3409
                     /locus_tag="VN084_01115"
     CDS             2378..3409
                     /locus_tag="VN084_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWT28082.1"
                     /translation="MRGLLIACLLLLSACSRDPLYQSQSYVFGTLVDISIYGEEPQRA
                     RALTDHILQDFQRLHDKLHAWHDNSALSQLNVAFAAGKAPVAIDDELATMLQDATKWS
                     VRSGGKFNPAIGQLIQQWGFQRDEFTPVHIEDAAIRQLVAANPQMTDIVIDKHMAYSR
                     NPAVKLDLGGYAKGYALDRAADYLRSQGVVNALVNIGGNIIALGKHGDKAWRVGIQHP
                     RAPGPIATLDLQDGWAIGTSGDYQRYFMRDDKRYCHIIDPATGYPGDQVQSVTVLIPP
                     AANSGVVSDVASKPIFLAPVDQRASAAMRMGIDNVMLIDAAGNIYSSAGMAKKLQWMD
                     PDVRWQTLF"
     gene            3384..3773
                     /locus_tag="VN084_01120"
     CDS             3384..3773
                     /locus_tag="VN084_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HWT28083.1"
                     /translation="MSVGKPYSEHALLSGDWVVIIGALLLIIASFRIFWHQEAAGKLL
                     IRQGDKVYARYSLDQDRTLDIHGPLGDSRIEIAHGKVRFHSSPCSNQYCVHQGWLSKV
                     GQVAICLPNQVSLELLGDSKPYDSLNY"
     gene            3790..4287
                     /locus_tag="VN084_01125"
     CDS             3790..4287
                     /locus_tag="VN084_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HWT28084.1"
                     /translation="MKITTTPDDHRIAKLTAIAIGLHLIESIVPSPLPGVKPGIANII
                     TLYVLYRYDFATAAWVSLLRIFAASLLLGQFLSPTFILSLSGGLCSLLVLGVASRLPL
                     RWFGPVSLSVISAFAHISGQLLIVRLLLIPNTGISYLIPVFATTALLFGVINGVIAGK
                     LLRHP"
BASE COUNT          894 a         1409 c         1220 g          883 t
ORIGIN      
        1 attaagcaaa atatgtttct tgatcgtcgc gtggttcaga atcaattctg ctcggtttca
       61 tgcatttatc tcctcctctt cgggcgcatc tagcgcccgt ttttttcgcc tgttggccgc
      121 aactcagcta aaatccagcc tatccttcct gtccttggtc agcttatggt cccgcatctc
      181 actaccgcac tgaatggccc cctactggaa cttgaaaagc gcatgctcgc cgccatgccg
      241 cagatcgagc attggttccg cacgcaatgg caggaacata ccgtgccgtt ctacgcctct
      301 gtggacttgc gcaatgccgg gttcaaactg gcaccggtag acaccaacct cttccctggc
      361 ggcttcaaca atctcaatcc ggatttcctg cccttatgtg tgcaggcggc gatgtcggcg
      421 gtagagaaga tctgcccgga ggcccgtcgt ctgctggtga tcccagagaa ccatacgcgc
      481 aacacctatt acctgcgtaa cgtcgccgcg ctcaccaaca tcctgcgaca ggccggcatg
      541 gaagtgcgca tcggcagcat ctcgccggag atcaccacca cgaccacgct tgatctgcag
      601 gatggccaaa cgctgcaact cgagcccgtg cagcgcaagg gcaaccgtct ggtgctggac
      661 gggttcgata gctgcgccat cctgctcaac aacgacctct ccggcggcgt acctgatgtc
      721 ctgcagcggc tggagcagga cctgatcccg ccgctgcacg ccggctgggc gacccgccgc
      781 aaatcacagc atttcagtgc ctataaccag gtggtggatg aattcgcaca actgctggag
      841 atcgacccgt ggttgctgaa tccctatttc gacacttgca gcgagatcga cttccacgcg
      901 cgcaccggcg aagcctgcct ctcgagccgc gtcgaaaagc tgctggagaa gatccggatc
      961 aaatatgcgg aatatggcgt caagcaggac ccgttcgtca tcgtcaaggc cgatgccggc
     1021 acctacggca tgggcatcat gacagtcaag agcccggacg atgtgcgcga cctcaatcgc
     1081 aagcagcgca acaagatggc tgtagtcaag gaaggccttg aggtcagcga agtcatcatc
     1141 caggaaggcg tctacacctt cgagaacctt aacgacgccg tcgccgaacc ggtgatctac
     1201 atgatcgatc actacgtcgt cggtggcttt taccgcgtac acaccggccg cgcagtggac
     1261 gagaatctca acgcgcccgg catgcatttc gtgccgctgg cattcgagcg ctcatgctcg
     1321 ctgcccgacc actcggccgc acctgacgcg ccaccgaatc gcttctatgc ctacggtgtg
     1381 gtcgcccggc tggctttgct cgcagcggcg atcgagctcg aaacccacgc gccggagcaa
     1441 tgatcgacat gcgcctgcta ttcattcttg atccgctgga ccagctgaag acatacaagg
     1501 acaccagcat cgccatcatg cgcgcggcgg cggcacgcgg ccatacgcta ttcaccgcca
     1561 tgcagggcga tatcctgttg cggcacgaac gtgtggtggt ccgcgccagc gcattccggt
     1621 tcgccgaagg cgcgagctgg tatgagggcg acgcagcatt cgagaccgtg cctgccgact
     1681 tcgatgctgt gctcatgcgc aaggatccgc cgttcgataa cgaatacctc tacagcacct
     1741 atctgctgga gctggcaacg cagcagggtg cacacgtcta caacgctcct gagtcgatcc
     1801 gcggctggaa cgaaaaactc tcgatcgcgc gtttcgccga gttcgcgccg ccctttctcg
     1861 tcaccgcgaa caatacgctg atccgcgatt tccttgccag ccacggtgac atcatcgtca
     1921 aaccgctcga cggcatgggt ggcagcggcg tgtttcgcct gcgcaccgac gatgccaaca
     1981 tcggcgtcat tctcgagacc gtgacgcact tcggcgaacg caccgtgatg gcccagcgtt
     2041 atgtgcccga gatcaaggtc ggcgacaagc gcatcctcat gatcgacggc caacccctgc
     2101 cttatgccct ggcccgtgtg ccgcaaggcg gcgagacacg cggcaatctt gctgccggcg
     2161 gcaaaggcgt ggcacagccg ctgagcccgc gtgaccttga gatcgccaac cggatcggcc
     2221 cggtgctgcg ggaacacggc ctattcctgg ttgggctgga tgtcatcggc gattacctga
     2281 ccgaggtcaa cgtcaccagc cccaccggca tggtggagat cgccgcgcag accgattgtg
     2341 accccgccag cctgttcatg gatgcgctgg aatctgcatg cgcggcctcc tgatcgcatg
     2401 cctgctgctt ctcagcgcct gtagcaggga cccgctctac cagagccaga gttatgtttt
     2461 cggcacgctt gtggacatca gcatctatgg tgaggagccg cagcgcgcac gcgcactgac
     2521 cgaccatatc ctgcaggatt tccagcggct gcatgacaag ctccacgcct ggcatgacaa
     2581 cagcgcgttg agtcagctca atgtggcctt tgccgccggc aaagcacctg tggcgatcga
     2641 cgatgagctg gcgacgatgc tccaggatgc gaccaagtgg tcggtgcgct cgggcggcaa
     2701 attcaacccg gccatcgggc aactcatcca gcaatggggt ttccagcgcg atgaattcac
     2761 gccggtgcat atcgaagatg ccgcgatccg gcaacttgtg gcagccaacc cgcagatgac
     2821 cgatatcgtc atcgacaagc acatggccta cagccgtaac cctgccgtca agcttgacct
     2881 tggcggctat gcgaagggct acgcgttgga ccgcgcagcc gattacctgc gctcgcaagg
     2941 agtagtcaat gccttggtga acatcggcgg caacatcatc gcccttggca agcacggcga
     3001 caaagcctgg cgtgtcggta tccagcatcc gagagcgcct ggcccgatcg ccacgctgga
     3061 tctgcaggac ggctgggcca tcggcacctc aggcgattat cagcgctatt tcatgcgcga
     3121 cgataaacgc tactgtcaca tcatcgaccc cgcgacaggc tatcccggcg accaggtaca
     3181 atccgtcacc gtgctcatac cgcctgccgc caatagtggc gtggtctccg acgtagcctc
     3241 caagccgatc tttctcgcac ccgtcgatca gcgcgccagt gctgcgatgc gcatgggtat
     3301 cgacaacgtc atgctgatcg atgcggccgg taatatctac agcagcgccg gcatggccaa
     3361 gaaactgcaa tggatggatc cagatgtccg ttggcaaacc ctattctgaa catgcgctat
     3421 tgtcggggga ctgggtcgtc atcatcggcg cattgctgct gatcatcgcc tccttccgca
     3481 tattctggca tcaggaagcc gccggcaaac tgctgatccg ccaaggcgac aaggtctatg
     3541 cacgttacag cctcgaccag gatcgcacgc tggatatcca tggcccgcta ggcgattcac
     3601 gcatagagat cgcgcacggc aaagtgcgct tccacagctc gccatgcagc aatcaatact
     3661 gcgtacacca gggctggctg agcaaagtcg ggcaagtggc gatatgcctg ccgaaccagg
     3721 tcagccttga gctgctaggc gacagtaaac cctatgactc cctcaattac tgaaccgccg
     3781 acacgcgcga tgaagatcac gaccacgccg gatgaccacc gcatcgcaaa gctgaccgcg
     3841 atcgccatcg gcctgcacct gatcgaatcc atcgtgcctt cccctttacc cggcgtaaag
     3901 cccggtatcg ccaacatcat cacgctctac gtgctgtacc gctatgactt cgctaccgca
     3961 gcctgggtga gcttgctgcg gatattcgcc gccagcctgc tgctggggca attcctgtcg
     4021 ccgaccttca tactgagcct cagcggcggc ctatgcagcc tgctcgtgct gggcgtggcc
     4081 agccgactgc ctttgcgctg gttcgggcca gtgagcctga gcgtgatatc agcctttgcg
     4141 catatttccg gtcaattgct gattgtgcga ctattgttga taccgaatac gggaatcagt
     4201 tatctgatcc ccgtattcgc tacgacggca ttgctgtttg gcgttataaa cggtgttatt
     4261 gcaggaaaac tgctcagaca cccttgagct aattacctga ccaactctct ccagcccgtc
     4321 ctgcgagatt gtgtactccc aggattggtc tggatggcga ccgtagactt acctccgcca
     4381 gcatcggttg cgccagcttt tagctg
//
LOCUS       DATMTW010000056         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_508365, whole genome shotgun sequence.
ACCESSION   DATMTW010000056 DATMTW010000000
VERSION     DATMTW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508365"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VN084_01130"
     CDS             complement(<1..219)
                     /locus_tag="VN084_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28085.1"
                     /translation="MNALLRYCLLASALLPWNATAIDTSDLGKFAAYGKFGANYTEGL
                     LVKSLIDVTQGHVQEALDTIDQLLKTTPN"
     gene            complement(254..1414)
                     /locus_tag="VN084_01135"
     CDS             complement(254..1414)
                     /locus_tag="VN084_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT28086.1"
                     /translation="MPVSRIYSISLTILLSLHLMADARADDLRDISRQASQGQQAAAL
                     ERINNYLSKNPSDVQAMFIRGVILADQGRRDDAIKVFTEITEKHPGLPEPYNNLAVLY
                     ADQGQYEKARKALETAIKTHPSYATAHENLGDIYARMASEAYDKALQLDTGNARAQNK
                     LAMIKDLFSTSTDKSTMLAAKASAAAKTPASVDKATPATPIRSAENKPVDPKPAAIVA
                     AADNAKPAPIKKAEPANTPALKPEPPKVEAAKPEPAKQAAAKAEQQTSATSGSADAEK
                     EAMAAVNAWARAWSSKDVDRYLASYAASFQTPDGQTRQQWEALRRERISKPARISVEL
                     SNIRAKLAGPDQARVSFKQRYQARGTSMRTSKSLVLKKNKGQWLIEQELTDR"
     gene            1577..2947
                     /gene="cysS"
                     /locus_tag="VN084_01140"
     CDS             1577..2947
                     /gene="cysS"
                     /locus_tag="VN084_01140"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWT28087.1"
                     /translation="MLKIYNSIAREKQVFVPIVAGKVSMYVCGMTVYDYCHLGHARVM
                     VVFDLVNRWLRASGFDVTYVRNITDIDDKIIKRANENNETIEALTSRFIQAMDEDAGK
                     LGVMRPDIEPKATTHIGAMLDMIAALIDKGHAYQADNGDVFYAVSSFAAYGKLSGKSL
                     DDLRAGERVEVDGYKRDPMDFVLWKSAKPGEPSWDSPWGKGRPGWHIECSAMGACHLG
                     KHFDIHGGGQDLQFPHHENEIAQSEGAHDCTFVNYWMHNGFVRVDDEKMSKSLGNFFT
                     IREVLKQYDAEVVRFFILRAQYRSPLNYSDQHLDDARQALTRLYTALRGQAVEAVEID
                     WQHPQAARFKAALDDDFNTPEAFAVLFELAGELNRTGTAETAGLLKALGGVIGLLQRS
                     PETFLQGGQSAHGYTPETIEQLITQRLAARQSKDFAEADRIRKELLDNGIVLEDGPQG
                     TVWRLA"
     gene            2940..3236
                     /locus_tag="VN084_01145"
     CDS             2940..3236
                     /locus_tag="VN084_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28088.1"
                     /translation="MPESIMTLARTLVVLLLLTLSFTALAQYTEHEPAQGQEQSLLAD
                     VDSIQSSSDCDLSDIGLLPGRRPGVHLPRIKACASLDKVYRQLAISPPFKPPVA"
     gene            3400..4653
                     /locus_tag="VN084_01150"
     CDS             3400..4653
                     /locus_tag="VN084_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWT28089.1"
                     /translation="MRAARRLLLAMLAGSLCLGGALQAHAMQTLTLQDAIDSALQQNP
                     SINIARAQEDAAVAGVTTAKAYINPEVEIGGGPTRYRRGTESNGNNYMIGLAQPLEFS
                     GVRSARRELAEAGVKVAEVGTDLARIDLRARVKSAFYDVLQREAVLKLVEGDRTLLKD
                     IRERVKLRVDVGESPRYELIKADTELLAAERDYQAALIRVAEGKAYLRGLIGPTVGDD
                     FELSGELPLTSSLPSLESLRERLEQSPQLAQIRAATEAADNRVRLEEALRMPGVTVKA
                     GVDQDPDMNSFHLGVAVPLPLWSQRQGQIAEAAAGAREVRSILSERELALRRDLDSSY
                     QRYLIARQQVSSFENGLLSQSEAVLKTAEAAYRYGERGILEYLDAQRTFRVVRKDYLT
                     ARYDYVNAMLEIERVLGTEILEDKP"
BASE COUNT          875 a         1417 c         1464 g         1024 t
ORIGIN      
        1 gttaggcgtg gtcttcagca gctgatcgat ggtatcgagc gcttcctgca cgtggccttg
       61 cgtcacgtct atcaggctct tgaccagcaa gccttcggtg taattggcgc cgaattttcc
      121 gtaggcggcg aatttgccga gatcggacgt gtcgatcgcc gttgcgttcc atggcaggag
      181 cgcgctggct agcagacagt aacgaagtag ggcgttcaat ttgcgcatca ccgtaggatc
      241 ggacgtggta tgcctagcga tcggtcagct cctgttcgat cagccactga cctttgttct
      301 ttttcaggac cagggatttc gatgtgcgca tcgaggtgcc gcgtgcctgg tagcgttgct
      361 tgaaactgac acgggcctga tccgggcccg cgagcttggc gcggatattg gacaactcca
      421 cactgatacg tgccggcttg ctgatacgct cacgacgcaa ggcttcccat tgctggcggg
      481 tctggccgtc cggggtctgg aaactggcgg cgtaactggc gagataacgg tcgacatcct
      541 tgctcgacca ggctctggcc caggcattga cggcagccat ggcttccttt tcggcgtctg
      601 ccgagccact tgtagctgaa gtctgctgtt cggctttggc ggctgcttgc ttggctggtt
      661 ccggtttcgc ggcctcgacc ttgggtggtt cgggcttgag ggcgggagtg ttggccggtt
      721 ctgccttctt gatcggagcc ggcttggcat tatcggcagc ggcgacgata gcggcgggtt
      781 tggggtcgac cggtttgttc tctgcagaac ggatcggcgt cgcaggcgtt gccttgtcga
      841 cgctggctgg cgttttcgcg gctgctgacg ctttcgcggc gagcatggtc gacttatcgg
      901 tgctggtgga gaacaggtcc ttgatcatgg ccagcttgtt ctgcgcgcgg gcattgccgg
      961 tatcgagctg cagcgccttg tcgtaggctt ccgatgccat gcgcgcatag atatcgccca
     1021 ggttctcgtg tgcggtggcg tagctcggat gggtcttgat tgcagtctcc agcgccttgc
     1081 gcgctttctc gtattgcccc tggtcggcat acaataccgc caggttgttg tagggctcgg
     1141 gcaggcccgg gtgcttttcg gtgatctccg tgaatacctt gatcgcgtcg tcccggcgac
     1201 cctggtcggc caggatcacg ccacggatga acatcgcctg cacgtccgag gggtttttgc
     1261 tcaggtaatt gttgatgcgc tcaagtgccg ctgcttgttg cccctggctg gcctggcggg
     1321 agatgtccct gaggtcatcg gcgcgtgcat cggccatcaa gtgcagggag agcaggatcg
     1381 tcaggctgat ggagtaaatc cgtgacacag gcattgtgct atacttttcg acattgattc
     1441 agccgccaat tctatcaaca agcacaatca acctccaata gctcgccatg ttttcagcag
     1501 atttatttct tggctatcaa attcttaagt caattattgc ttccgctgct taaggttcat
     1561 cccgctcacc agtgccatgt taaaaatcta taattcgatc gcgcgcgaga agcaggtctt
     1621 cgtacccatc gtcgccggca aggtcagcat gtacgtttgc ggcatgacgg tctatgacta
     1681 ttgccatctc ggccatgcgc gcgtcatggt ggtgttcgac ctagtcaatc gctggttgcg
     1741 cgcctccgga ttcgacgtga cctatgtgcg taacatcacg gacatcgacg acaagatcat
     1801 caagcgggcc aacgagaaca acgaaaccat cgaggcgttg accagccgtt tcatccaggc
     1861 catggacgag gatgccggca agctcggcgt catgcgaccg gatatcgaac ccaaggccac
     1921 gacccacatc ggcgccatgc tcgacatgat cgccgcgctc atcgacaagg gccatgccta
     1981 tcaggctgac aacggcgacg tgttctatgc cgtaagcagc tttgccgcct atggcaagct
     2041 ctctggcaag agtctcgatg atctgcgcgc cggtgagcgt gtcgaagtcg atggctacaa
     2101 gcgcgacccg atggatttcg tgctctggaa gtcggccaag ccaggcgagc ccagttggga
     2161 ttcgccctgg ggcaagggcc ggcccggttg gcacatagaa tgctccgcca tgggcgcctg
     2221 ccatctgggc aagcatttcg atatccacgg cggcggtcag gatttgcaat tcccgcatca
     2281 cgagaatgag atcgcgcaat ccgagggcgc gcatgattgc accttcgtca attactggat
     2341 gcacaacggg ttcgtgcgcg tcgatgacga gaagatgtcc aaatcgctgg gcaacttctt
     2401 caccatccgc gaggtattga agcaatatga cgccgaagtc gtgcgtttct tcatcctgcg
     2461 cgcgcaatac cgcagcccgc tcaattattc cgaccagcac ctcgacgacg cccggcaggc
     2521 actgacacgg ctctataccg ccttgcgtgg gcaggccgtc gaagcggtgg agatcgactg
     2581 gcagcatcca caggccgccc gtttcaaggc agcgctcgat gacgacttca atacgccgga
     2641 agcctttgcc gtgctgttcg agctcgccgg tgagctgaac cgcaccggga ctgccgagac
     2701 agcgggtttg ctcaaggcgc ttggcggcgt catcggtctg ctgcaacgct cgccagaaac
     2761 ttttctgcag ggcggacagt ctgcgcatgg gtacactccg gaaaccatcg aacaattgat
     2821 cacccaacgc cttgccgcca ggcaatccaa ggatttcgcc gaggccgacc gtatccgcaa
     2881 ggaattgctg gataacggca tcgtgctcga agacggcccg caaggcaccg tctggcgtct
     2941 tgcctgaatc catcatgacc cttgcccgca ccctggtcgt attgttgctg ctgacgctgt
     3001 ctttcacggc gctggcccaa tacaccgagc atgagccggc gcaggggcag gagcagtctt
     3061 tgctcgctga tgtcgatagc atccagtcgt ccagcgactg tgatctcagc gacatcggtc
     3121 ttttgccggg gcgccgcccc ggtgtccacc ttccgcgtat caaggcatgc gccagtttgg
     3181 ataaggtcta tcgccagctg gccatctccc cgccgtttaa accgccagtc gcctgagtga
     3241 ctttcgggct ggcgcgttaa ttgctaagct tttggcttgc actatgcgca ccttatccaa
     3301 gcttttcctg acgatggccg cccattcttg ggcgggtatc tcgtcaggtt caccgaagaa
     3361 ctgagacata tgacatcacc atccgtaatt ggacagccga tgcgcgccgc gcgccggctt
     3421 ctgttggcca tgcttgccgg cagcctgtgt ctgggcggtg cgctccaggc gcatgccatg
     3481 cagaccctga ccctgcagga cgcgatcgac agcgctctgc agcagaatcc ttccatcaac
     3541 atcgcgcgag cgcaggaaga cgccgccgtc gccggtgtca ccacggccaa ggcctacatc
     3601 aaccccgaag ttgagattgg cggcgggccg acccggtatc gccgcggcac cgagagcaat
     3661 ggcaacaact acatgatcgg cctggcgcag ccgctggaat tctcgggtgt gcgttcagcg
     3721 cgccgtgaac tggcagaggc cggcgtcaag gtcgccgagg tcggcaccga tttggcgcgt
     3781 atcgacctgc gtgcccgcgt caagagcgcg ttttatgacg tgctgcagcg tgaagcggtg
     3841 ctgaaactgg tcgagggtga ccgtaccctg ctcaaggaca tccgggagcg ggtcaagctg
     3901 cgcgtcgatg tcggtgaatc gccacgttac gagctgatca aggccgatac cgaattgctc
     3961 gcggctgagc gggattatca ggccgccctg atccgtgtcg ccgaaggcaa ggcctatctg
     4021 cgcggcctca tcgggccgac ggtcggcgac gatttcgagc tcagcggcga attgccgttg
     4081 accagtagcc tgccttcgct ggagtcgctg cgcgagcgct tggaacaaag cccgcaactg
     4141 gcacagatcc gggccgccac cgaagcggcc gataaccgcg tgcggctgga agaagcgctg
     4201 cgtatgcccg gtgtcacggt caaggctggc gtcgatcagg atccggacat gaacagtttc
     4261 catcttggcg tggccgtccc gctgccgttg tggagtcagc ggcaagggca gatcgccgaa
     4321 gccgccgctg gcgcgcgcga ggtccggtcg attctcagcg agcgtgaact ggcgctgcgc
     4381 cgggatctgg attcgtccta ccagcgctat ctgatcgccc gtcagcaggt gtcctccttc
     4441 gagaatgggc tcctgagcca gtcggaggcc gtcctgaaaa cggcggaagc cgcatatcgc
     4501 tacggcgagc gcggcatact ggaatacctg gacgcacagc ggaccttccg cgtggtgcgc
     4561 aaagattacc tgacagcacg ctatgactat gtgaatgcga tgctggagat cgagcgtgtg
     4621 ctcggaaccg aaatcctgga ggataagcct tgaagtcttt tctgatcgat acctacaatc
     4681 ccgccaccgg ctccgcacga gcagtggtca gtctcctact ggccctgacg ctcgccggtt
     4741 gcggcaagac cgaggtcgaa gcgcctgcca ccacgcaggt
//
LOCUS       DATMTW010000057         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_440812, whole genome shotgun sequence.
ACCESSION   DATMTW010000057 DATMTW010000000
VERSION     DATMTW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440812"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VN084_01155"
     CDS             complement(<1..546)
                     /locus_tag="VN084_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HWT28090.1"
                     /translation="MERTIVQKNAQRVRLLRGLRKIHGWLGLWGAVLGLLFGVSGFLL
                     NHRAVMKIPAARMEATEIQLGMAQPYPQDQKAFVRYIQQALDISQAPARPKGRPPQKE
                     TMAQTSEPARHGKVEDQATTQTSAAGMGSSGASAREGASVVKTAGKAEHDTRFMGQAM
                     KQPAVWTVEFQLPQASIKAEYI"
     gene            complement(568..1242)
                     /locus_tag="VN084_01160"
     CDS             complement(568..1242)
                     /locus_tag="VN084_01160"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HWT28091.1"
                     /translation="MLIVLDDVLNAQALREFRAAYDQLPYGDGRATAGERAAEVKRNL
                     QLANDTPQHAALTASVHAALGSCAEFMSAALPLQISPPLFNRYLPGMAFGQHVDNAIR
                     IDGEQPLRADVSATLMLSGPEEYQGGDLVIADTYGEQRIRLKAGSLVVYPSSSLHRVE
                     AVTSGQRDVIVFWTQSMVRDNGQRATLYRLDQTIRQLCQRQPDAPEIDALMATYNNLL
                     RMWADL"
     gene            complement(1333..>2916)
                     /locus_tag="VN084_01165"
     CDS             complement(1333..>2916)
                     /locus_tag="VN084_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWT28092.1"
                     /translation="ALNDVVSVRLNALWNDSDSFRDQNFTRRHGLAPSIKFQFSDKTD
                     LTLNYLYQKEDSVFDYGVPMFLGRPADVSTRKFYGFKDDRLQEYETNVATATLKHAFT
                     DEFSVKNTTRYGDYYRKYRTNLVSSVGNDPAITTTFGTATFSQALRDSHQQNLINQTD
                     FLLTKPLFGMENKLAFGAEFGEENYDFRSKNSPGAAYRTSIFNAVFPATWPNGTGAAD
                     DFSGTLNSSRQIRAITKAAYVQDQLSLTEHWKVLAGVRYDDFRVDQTDVLVPANNAFD
                     SGNHKWSPRTGLIWQPTEAQSYYVSYGESFNPTAEQYALIDTAEEAKLKPETSSNKEI
                     GAKWDLFGGRVSLTSAIYRLEKDNARTTNPLTGLTTLSGKQRTDGFELGVNGAVTSNW
                     NVSAAYSYMEAEVIESLQTATGSVSGTTDIPLKGMTPQNVPKNSGTVWTTYRFMRNWE
                     VGGGLYAVGSRFTDNVNEVELPGYVRLDAVLAYHQPKYDVQLNLFNLLDKKYYESGQV
                     RSALPGLPLSGMLSLNLRY"
BASE COUNT          522 a          847 c          920 g          627 t
ORIGIN      
        1 gatgtattcg gccttgatgg aagcttgcgg tagctgaaat tccacggtcc acaccgctgg
       61 ctgcttcatc gcctgcccca tgaaccgggt atcgtgctcg gccttgcctg cggttttcac
      121 cacactggca ccttctctgg ccgaggcacc tgatgagccc attcccgcgg cgctagtctg
      181 cgtcgtagcc tgatcttcaa ccttgccgtg ccgcgcaggc tccgatgttt gcgccatggt
      241 ttccttttgc ggtggtcggc ctttggggcg cgcaggtgcc tggctgatgt ccagggcctg
      301 ctgaatgtag cgcacaaagg ctttctggtc ttgcggataa ggctgggcca tccccaactg
      361 gatctcggtc gcttccatcc tggctgccgg gatcttcatc acggcgcgat ggttgagcag
      421 gaacccactg acgccgaaca acaggcccag caccgccccc caaagcccaa gccagccatg
      481 gatcttgcgc aggcctctca gcaggcggac gcgttgtgca ttcttttgta cgatggttct
      541 ttccataagg tttcttcaaa aaagggttca gaggtctgcc cacatgcgca gcaggttgtt
      601 ataggtcgcc atcagggcgt cgatctccgg cgcgtccggt tgccgctggc acagttggcg
      661 gatggtctgg tcgaggcgat acagcgtggc gcgctggcca ttgtcgcgca ccatgctctg
      721 cgtccagaac acgatcacat cacgctggcc ggatgtcacc gcttcgaccc ggtgcaggct
      781 ggacgacggg tagacgacga gactgcctgc cttgagccgg atgcgttgct cgccgtaggt
      841 gtcggcgatc accaggtcgc cgccctggta ctcttccgga ccggacagca tgagcgtcgc
      901 cgagacatcg gcccgtaagg gctgctcgcc atcgatacgg atggcattgt ccacatgctg
      961 gccgaaagcc atgcccggca ggtagcgatt gaacagcggc ggactgatct gtaacggcaa
     1021 ggccgccgac atgaattcgg cacagctgcc gagggcagca tgcacgctcg ccgtcagcgc
     1081 cgcgtgctgc ggcgtatcgt tggccagctg caagttgcgc ttgacctcgg ccgcgcgctc
     1141 ccccgccgtc gcccggccgt cgccgtacgg caactggtcg taggcggcgc ggaattcgcg
     1201 caaggcttgt gcgttcagta catcatcgag aacgatcagc atgcgggttg cactcctgta
     1261 cggttcaatc aaaaaaagcc cggcatggct gccgggcaag tgtcgcatgc atgaggagaa
     1321 agcgatgcgg catcaatatc tcaggttgag cgaaagcatc cccgagagtg gcaagccggg
     1381 cagcgcgcta cgcacttgcc ccgattcata gtatttcttg tccagcaggt tgaacaggtt
     1441 gagctgcaca tcatacttgg gctgatggta tgccagcacc gcatcgaggc gcacgtaacc
     1501 tggcaactcg acttcattca cattgtcggt gaaccggcta ccgaccgcat agagaccgcc
     1561 gcccacttcc cagttgcgca tgaagcgata ggtggtccat acggtaccgc tgttcttcgg
     1621 aacattctgc ggcgtcatgc ccttgagcgg gatatcggtg gtgccgctga cggaacccgt
     1681 ggcggtctgc agcgactcga tgacttccgc ctccatgtag gagtaagcgg cggagacgtt
     1741 ccagttgctg gtgacggcgc cgttgacgcc gagctcgaaa ccatccgtgc gctgcttgcc
     1801 ggagagcgtg gtgaggccgg tcagcgggtt ggtggtgcgg gcgttgtcct tctccagccg
     1861 gtagatggcg gaggtaaggc tgacgcggcc gccgaacagg tcccacttgg cgccgatctc
     1921 cttgttgctg ctggtttccg gcttgagctt cgcttcttcc gcggtatcga tcagcgcgta
     1981 ctgctcggcg gtcgggttga acgattcgcc gtagctcacg taataggatt gcgcctcggt
     2041 cggctgccag ataaggccgg tgcgcggact ccacttgtgg ttgccactat cgaaggcgtt
     2101 gttggctggt accagcacgt cggtctgatc gacccggaaa tcgtcataac gcacgccggc
     2161 cagcactttc caatgctcgg tcagggagag ctgatcctgc acgtaggcgg ccttggtgat
     2221 cgcgcggatc tggcggctgc tattcagcgt gccgctgaaa tcatccgctg cgccggtgcc
     2281 gttgggccat gtcgcaggga agaccgcatt gaagatggaa gtacgataag cggcacctgg
     2341 cgagttcttg ctgcggaaat cgtagttctc ttcgccgaat tcagcgccga aagccagctt
     2401 gttctccatg ccgaacagcg gcttggtcaa caggaaatcg gtctggttga tgaggttctg
     2461 ctgatggctg tcgcgcaacg cctggctgaa ggtggcggta ccgaaggtcg tcgtaatggc
     2521 cgggtcatta cctacgctgc tgaccagatt cgtgcggtat ttacgatagt aatcgccgta
     2581 gcgcgtggtg ttcttgaccg agaattcgtc ggtgaaggca tgcttcagcg tggcggtggc
     2641 gacgttggtc tcgtattcct gcaggcggtc gtccttgaag ccgtagaact tgcgcgtgga
     2701 gacatcggcc ggccggccaa gaaacatggg cacgccgtaa tcgaagacgc tgtcttcttt
     2761 ctggtagagg taattcaggg tcagatcggt cttgtcgctg aactggaact tgatcgatgg
     2821 cgcgaggccg tggcggcggg tgaagttctg gtcacggaac gaatccgaat cgttccatag
     2881 ggcgttcaaa cgcaccgaga ccacatcatt caaggc
//
LOCUS       DATMTW010000058         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_440851, whole genome shotgun sequence.
ACCESSION   DATMTW010000058 DATMTW010000000
VERSION     DATMTW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440851"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VN084_01170"
     CDS             <1..590
                     /locus_tag="VN084_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWT28093.1"
                     /translation="AEVWLADKGWTRVDPTAAVAPQRVNAGIDAALPDNALGPVFARK
                     DYPLLRKLYLNWDAVNNGWNQWVLGYDQEKQMALLNRLTGSDLSWQDLVIGLVLSLAT
                     VGLILSAFLLRGKRRILDPVQRLYVQFLRKLERAGLKRLAHEGPQDFGHRAARRLPDR
                     AKEIEAITQAYTGIRYRSNISAEALAALRHMIKAF"
     gene            641..1099
                     /gene="tadA"
                     /locus_tag="VN084_01175"
     CDS             641..1099
                     /gene="tadA"
                     /locus_tag="VN084_01175"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWT28094.1"
                     /translation="MTDEEYMQIALELAQQAAEAGEVPVGAVIVRDGEIIGRGSNAPI
                     ASHDPTAHAEIQAMREAAAAIGNYRLVGCTLYVTLEPCAMCSGAIQHARIARLVYGAS
                     DPKTGACGSVVDLMAESRLNHHTVVERGVLADTCGATLSAFFAARRRKPG"
     gene            complement(1247..1936)
                     /locus_tag="VN084_01180"
     CDS             complement(1247..1936)
                     /locus_tag="VN084_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWT28095.1"
                     /translation="MQTNYQVANSTQALSPESHKVLRNTYMMLGLTMVPTVIGAVVGM
                     SMNFAWAAQHPIMFAIGSLALIFGLFFGISANRNSSLGVVLLLALTFILGMMLGPILQ
                     VALQLRNGAQLVGLAAGGTGIIFLTLAGIATTSKKDFSFLGNFLLVGIILLIIASLAN
                     MFFQVPAIALALSGVAVLLFSGFILYDVSRIVNGGETNYVMATLAIYMDIYNLFVNLL
                     QLLMALLGERD"
BASE COUNT          451 a          670 c          681 g          370 t
ORIGIN      
        1 gggccgaggt atggctggcg gacaagggct ggacgcgggt cgacccgacc gctgccgtgg
       61 cgccgcaacg cgtcaatgca ggcatcgacg ccgcactgcc ggataacgcg ctcggcccgg
      121 tgttcgcgcg caaggattac cccctgctgc gcaagctgta cctcaactgg gacgcggtca
      181 acaacggctg gaaccaatgg gtcctcggct atgaccagga aaagcagatg gcgctgctca
      241 accgcctcac cggcagcgat ctcagctggc aggacctggt gatcggcctc gtgctgagcc
      301 tcgcgaccgt tggcctgatc ctcagcgcct tcttgctgcg cggcaaacgc cgcatactgg
      361 atccggtgca acggctctat gtgcagttcc tgcgcaagct cgaacgtgcg gggctcaagc
      421 gcctggcgca cgaaggcccg caggatttcg gccaccgcgc ggcgcggcgc ctgccggatc
      481 gcgccaagga gatcgaagcc atcacgcagg cctataccgg tatccgttat cgcagtaaca
      541 tcagcgccga ggcgctggcg gccttgcgcc acatgatcaa ggcgttctag caagcgatgg
      601 ccatgccggc gatacatgcc acattcaaaa agacacgacc atgaccgatg aggaatacat
      661 gcaaatcgcg ctggaactgg cgcagcaggc tgccgaagcc ggcgaagtcc cggtcggtgc
      721 cgtcatcgtg cgtgatggcg agatcatcgg ccgcggcagc aacgcgccta tcgcaagcca
      781 tgacccgacc gcgcacgccg agatccaggc catgcgtgaa gcggcggcgg ccatcggcaa
      841 ctatcgtctg gtcggttgta cgctttacgt gacgctggag ccctgcgcca tgtgcagtgg
      901 cgccatccag catgcgcgca tcgcacggct ggtatatggc gccagcgacc ccaagaccgg
      961 cgcctgcggc agcgtggtcg acctcatggc cgaaagccgg ctcaaccatc acacggtggt
     1021 cgaacgcggc gtgctggccg acacctgcgg cgcgacgctc tccgcgttct tcgcggcccg
     1081 ccgtcgcaaa cccggctgat ctagcgcatc tgtcgtcggg tcgtcctgcc cgttggcaac
     1141 actagtccag actcatcgca tcgcctgcaa tctgcctgac cgaaacgcca gccataaaaa
     1201 acccgccacg agggcgggtt cgcttgccag cggataagcc gttagctcaa tcgcgttcgc
     1261 cgagcagcgc catgagcaac tgcagcaggt tgacgaacag attgtagatg tccatgtaga
     1321 tcgccagcgt cgccatcaca tagttggtct caccaccgtt gacgatgcgg ctgacgtcat
     1381 acaggatgaa cccggagaac agcagcacgg caacgccaga cagcgccagc gcgatggccg
     1441 gcacctggaa gaacatgttg gccagcgagg cgatgatcag caggatgatg ccgaccagca
     1501 ggaaattgcc gaggaagctg aaatccttct tggacgtggt agcgatacca gccagcgtca
     1561 ggaagatgat gcctgtgccg ccagcagcga gaccgaccag ttgtgcgccg ttacgcagct
     1621 gtagggcaac ctgcaggatc gggcccagca tcatgccgag aatgaaggtc agcgccagca
     1681 gcaggacgac accgaggctg ctgttgcggt tggcggagat accgaagaac agaccgaaga
     1741 tcaaggccag cgagccgatg gcgaacatga tcgggtgttg cgcagcccat gcgaaattca
     1801 tgctcatgcc gaccacagcg ccaatgacgg taggcaccat ggtcaggccc agcatcatgt
     1861 aggtattgcg caataccttg tggctttcgg gagaaagcgc ctgggtactg ttcgcgactt
     1921 gatagttggt ttgcattgcg tgtgtttcct cgtgtgaata aaacctgtgt gtaagactag
     1981 ccaaacccgg ccgaagtttc aatgggattt atgtgacaga ttgtgtccgg gccctatacg
     2041 gctcaagtga tgaacggcag gttgggctcg gtcttgccga cctgcccgac ctggtggatg
     2101 gcctgcatga acgccgcgtc ctcgcgcaac gcgaccatgt cggccggatg atggcggcca
     2161 tgcatgcgtg cc
//
LOCUS       DATMTW010000059         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_474966, whole genome shotgun sequence.
ACCESSION   DATMTW010000059 DATMTW010000000
VERSION     DATMTW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474966"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VN084_01185"
     CDS             complement(<1..329)
                     /locus_tag="VN084_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWT28096.1"
                     /translation="MSAEHRIAVIAGDGIGQEVMPEGMRVLEAAGRRFGISFKWDELD
                     WSCERYHQTGSMMPEDGLDRIRHHDAIYLGAVGYPGVPDHISLWGLLIPIRREFDQYA
                     NVRPVRL"
     gene            complement(326..1270)
                     /locus_tag="VN084_01190"
     CDS             complement(326..1270)
                     /locus_tag="VN084_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HWT28097.1"
                     /translation="MRILCLGAGAIGGYFGGRLVAAGAADVTFLVREKRQMQLRADGL
                     RIESTYGDAKVNVTALTRAEIDGAYDLILLTCKAYDLPSAIETIEGLVGRDTAVLPLL
                     NGVAHIDILNAKFGRERVLGGVAKIAAMLTPDGVIKHLNDWRYITFGEQDGALSERVL
                     ALKAAFDKTSVVAAAVPNILHVMWEKVVHLSTAAGMTCAMRASVGEIARTRLGAALML
                     DFLERNAEIARREGFPPSDAFMQEYRQLFQNRSSTYTASMLRDILRSGPIEADHVLGF
                     MLDKAIAHKVDAELHRFVYVNLQSYEEHRAATALKVAS"
BASE COUNT          367 a          541 c          444 g          296 t
ORIGIN      
        1 atcaaccgca ccggacggac gttggcatac tgatcgaatt cccgccggat cggaatgagc
       61 agcccccaaa gcgagatgtg atcgggcacg cccggatagc cgaccgcgcc gagataaatc
      121 gcatcatgat ggcgaatccg atcgaggcca tcctcgggca tcatgctccc ggtctggtga
      181 tagcgctcgc acgaccagtc gagttcgtcc cacttgaagg aaatgccgaa ccgtcgcccc
      241 gcagcctcca atacgcgcat gccttccggc atgacctcct ggccgatccc atcgcctgca
      301 atcaccgcga tgcgatgctc ggcgctcatg acgccacctt cagcgcagtc gcagcccgat
      361 gctcctcata ggactgcaga ttgacgtaga caaagcgatg caactccgca tcgaccttgt
      421 gcgctatcgc cttgtcgagc atgaagccaa ggacgtgatc cgcttcgatt ggcccgctcc
      481 ggaggatatc tcgcagcatc gaggccgtgt aggtcgacga gcgattctgg aagagctgcc
      541 gatactcctg catgaaggca tccgaaggtg gaaaaccttc acgccgcgcg atttcagcgt
      601 tgcgctccag gaaatccagc atcaacgccg cacccagccg tgtccgcgca atttcgccga
      661 cactcgcgcg catcgcgcaa gtcatgccgg ccgcggtgga gagatgcacg accttctccc
      721 acatcacatg caggatgttc ggcacggccg cggcaacaac ggacgtcttg tcgaatgcgg
      781 ccttaagcgc cagcacacgc tcgctgagag caccgtcctg ctcgccgaac gtgatgtaac
      841 gccaatcatt gagatgcttg atgacgccgt ccggcgtcag catcgcggcg atcttggcaa
      901 cgccgcccag cacccgttcg cgaccgaatt tggcgttcag gatatcgata tgcgccacgc
      961 cattgagcag cggcaacacc gcggtatcgc gcccgaccaa cccttcgatg gtttcgatcg
     1021 cggaaggcag atcataagct ttgcatgtca gcaggatcag atcgtatgca ccatcgatct
     1081 cggcgcgggt cagcgccgtg acgttgacct tggcatcacc gtaggtgctt tcgatccgca
     1141 ggccatccgc gcgaagctgc atctggcgct tctcccgcac caggaaggtg acgtcagcgg
     1201 ctcccgccgc aaccaaacgc ccgccgaaat agcctccgat ggcgcctgcc cccagacaca
     1261 gaatgcgcat tacttccccg atcgtcacga cagcggaagc caccttcggc gaccgcgtca
     1321 cctatcataa tcatattgcg cgttgatagc atggcaattt aacatgtcaa tacatcatat
     1381 cgccgacacc agggataagc agatccggat gcgccagccg gaggccgcgc gcgccagacg
     1441 ccgcgtcgat ttagtcgacg agaatgcggt ccggcgtaaa ccctctacgg attctgcggc
     1501 cagcggaatc ccagatcata ttcgattcaa aaacgcgatc ttcgaacctg attggatctt
     1561 gcacgctcgc cccgcaacac agaatgctgc gctgtgaacc agccaacgcg ttgcaaaggt
     1621 taaaccggaa cgcatagcaa aaatgcca
//
LOCUS       DATMTW010000060         7124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_72377, whole genome shotgun sequence.
ACCESSION   DATMTW010000060 DATMTW010000000
VERSION     DATMTW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72377"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VN084_01195"
     CDS             complement(<1..1013)
                     /locus_tag="VN084_01195"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWT28098.1"
                     /translation="MSKLLIVESPSKAKTLKKYLGKDFEVLASYGHVRDLIPKTGAVD
                     PDNDFAMKYEVIDRNARHVDAIAKAVKNADAIYLATDPDREGEAISWHIAEILKSKKL
                     VKDKLMKRVVFHEITKSAVNEAIAQPRDISMPLVNAQQARRALDYLVGFNLSPLLWKK
                     IRRGLSAGRVQSPALRLIVERELEIEAFKRREYWTVHLESRKHQHAFDAKLIQLDGNK
                     VEQFTVTSQDQEREIVGKLLLASAGKATVSRVEKKQKSRSPAAPFTTSTLQQEAVRKL
                     GFTTSRAMRIAQQLYEGIDIGGGTVGLITYMRTDSMSLATEAVMQIRDYIKKHFEADY
                     LPKS"
     gene            1133..3001
                     /locus_tag="VN084_01200"
     CDS             1133..3001
                     /locus_tag="VN084_01200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWT28099.1"
                     /translation="MKRDGLSRHLLLACAISALLLVEATWLHSLQPLENRLSDVLLRQ
                     HALHSPPDPDIVIVDIDERSLALMADSVGRWPWPRSMHAELIEGIARQQPKAIVFDIL
                     FSDPDLTRPQDDAYFAEVIGRFDQLYFPMLLLDGADTGKGIPLREYGSRLGAVPGPQA
                     EPQATAAMVLPLPAILATGRVGTHNAIADQDGVVRSYPIYMYAGGWRIPSLPARVAVD
                     LGYALPATGEMPLHWHGGVLAYPRVSYADLYADLQRRVSQRPAAEFKGKIVIIGATAT
                     GLHDVRATPVDGFYPGVAILATALDNLKNADSLKPAPLAWGLGGGMLAILLLALAFAV
                     RRHIAVIGLALGAASALALLTAYLVLQAQWRVWVLTPVLFAWLYFLVAAFMAYWQERK
                     TRERAIATFSRFLDPRVVDALVAQGVTLASLGGKSRQVSVLFSDIRGFTTLSEGSTPE
                     QVVELLNAYFTLQAEAVFRQEGTLDKYIGDAIMAFWGAPAEQPDHALRAVAAALDMSD
                     KLEQFRAQLVAQDKPLGQALEIGIGVHSGPAVVGFIGSPNKQEYTAIGDTVNLTSRIE
                     GQTKGICRVLVSEDTRQLCELQANGHCPYDFVDHGVYEVKGRARPVRLFEPKRKAG"
     gene            3027..3575
                     /locus_tag="VN084_01205"
     CDS             3027..3575
                     /locus_tag="VN084_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWT28100.1"
                     /translation="MKSRDLILVGLLLMPPWVLAGEPAQTLVAADLKREPYADAATIL
                     SLPAASAVEVIKRQGGWMHVKSADGKEGWLKMVSIKLGAGAAAKGDTGLGSLLNVARS
                     GRSGNTGVTVATGVRGLSAEDLKNAKPAPEALKRLDSLPNGKREGESFASAGRLQAQK
                     IDYLGMSQAATDQGANTFGGGR"
     gene            3578..4414
                     /locus_tag="VN084_01210"
     CDS             3578..4414
                     /locus_tag="VN084_01210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWT28101.1"
                     /translation="MKRVLVLIVLCISAATAQAFELNINKLVDAIGKVKDLKPVDDKG
                     EAEIGSGVAANLLGAAPLVQDDKLQAYVSRLGWWLAMQTERADLSWRFGVLDSDTVNA
                     FAAPGGYVFITRGLLLRMRNEAELAGVLSHEIAHVLKRHHLNAIQKNARTGLMADLAS
                     MAASTSEHAALAEKAIGAGTELYTRGLDKDDEFEADRMGVVIAARAGYDPYGLPAVLQ
                     TLEAANAQDSNLALMFKTHPAPQQRLELLDGLMTGTLDRYASQPVVADRFMKVVGDYA
                     KR"
     gene            complement(4490..4933)
                     /locus_tag="VN084_01215"
     CDS             complement(4490..4933)
                     /locus_tag="VN084_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28102.1"
                     /translation="MMHKPFDDNADLAAREALVSLFLPLALTLETDHFRVFLAVCMED
                     GISLQKLGRLTDLGQSEVIRATSMLETWQAGPDACGLLHNEMALAKGYRRLSFLTASG
                     KALRAALQDAFDTEEYAEAAAAFHSFMQQELSAGFSETAAETVQA"
     gene            complement(4938..>7124)
                     /locus_tag="VN084_01220"
     CDS             complement(4938..>7124)
                     /locus_tag="VN084_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HWT28103.1"
                     /translation="PEHASSFEFCENGVKAVAAEATSRRVTPAFFAKHSVTSLLAQSD
                     FWLENQGRLTHPMVYNAATDHYEAISWDNAFQKIASALQALDHPDQAAFYTSGRASNE
                     AAFLYQLFVREFGTNNFPDCSNMCHEASGYALIESIGIGKGTVKLEDFHKADAIFIFG
                     QNPGTNHPRMLGELRSAAKRGCKIVSINPLKERGLEKFAFPQSPVEMLTGGSTPISSL
                     YLQPNIGGDLAVIKGMLKHIIAQHDAALANGQPGVIDQAFIDRHTHGFESFLADIRTE
                     RWDVILEESGISRAEIEAAANIYLASPRVICTWGMGLTQHKHSVLTIQQVVNLLLLGG
                     HIGREGAGACPVRGHSNVQGNRTVGITEKPSQAFLDRMEQVFGAKMPRENGLDVVATI
                     EAMQRGDVRVFVGLGGNFAAATPDTTLTEAALRRCHLMVNISTKLNRSHLVMGKQALI
                     LPCLGRTEIDLQASGPQMVTVEDSMSMVHGSGGINKAASPHLLSEPAIVARMAHATLS
                     GSRVQWLDLIADYDRIRDLIEKVYDGFHDYNTRVRRPGGFYLPNSAANHDWRTATGKA
                     NFTTAAIPTDLPFHQATRTSEAPVFNLMTTRSHDQYNTTIYGLDDRYRGVFGQRRVIF
                     ISRKDLDKLGMQADQWVDLRTVSDDGVERKAEQFRLVEYDIPEGCIAGYYPETNPLVP
                     IGALGDFSRTPTSKSIPVTLCLSAIQSPNGKSSGSSEKHPDELAAA"
BASE COUNT         1280 a         2101 c         2322 g         1421 t
ORIGIN      
        1 gacttcggca agtagtcggc ctcaaagtgc ttcttgatgt agtcgcgaat ctgcatcacg
       61 gcctcggttg cgaggctcat ggagtcggtt cgcatgtagg tgatgaggcc gacggtaccg
      121 ccgccgatat cgatcccttc atacagctgc tgtgcgatgc gcatggcacg gctggtcgtg
      181 aaaccgagct tgcgcacggc ttcctgctgc agggtcgagg tggtgaacgg ggcagccggg
      241 ctgcggctct tctgcttttt ctccacgcgc gacacggtgg ctttgcccgc actggcaagc
      301 agcagcttgc cgacgatctc gcgttcctgg tcctgcgaag tgacggtgaa ttgttccacc
      361 ttgttgccgt cgagctggat caatttcgca tcgaaggcgt gctgatgctt gcgcgattca
      421 agatgcacgg tccagtattc gcggcgcttg aaggcttcga tctccagctc gcgctcgacg
      481 atgaggcgca gtgccgggct ttgtacacga ccggcagaca agccgcgacg gatcttcttc
      541 cacagcagcg gcgacaggtt gaagccaacc aggtaatcga gcgcacggcg tgcctgctgc
      601 gcattgacca ggggcatgga gatatcgcgc ggctgggcga tcgcttcgtt cacggcgctc
      661 ttggtgatct catggaacac cacgcgcttc atgagtttgt ccttgaccag tttcttgctc
      721 ttgaggatct cggcgatatg ccaggagatg gcctcccctt cgcggtccgg gtcggtcgcc
      781 aggtagatgg cgtcagcgtt cttgactgcc ttggcgatgg catccacgtg ccgggcgtta
      841 cggtcgatga cttcgtattt catggcgaag tcgttgtccg ggtcgacagc gccagtcttg
      901 gggatcaggt cgcgaacatg cccgtacgaa gccagcactt cgaaatcctt gcccaggtat
      961 ttcttcagcg tcttggcttt ggagggagat tcaacgatca gtagtttgga catgtacaca
     1021 atttgtggga tacaggagcg gcagataata agagtaaatc actgccaagt gcaagaaacc
     1081 tctgaaataa gagtgctttg gcatcaacag gttcgccagt tatgatgcgt gcatgaaacg
     1141 cgacggccta tcccgccacc ttctcctcgc atgcgcgatc tcggccctgt tgctggtgga
     1201 ggcgacctgg ctgcatagtc ttcagccgct ggagaatcgg ttgtccgatg tcctgctgcg
     1261 tcagcacgcc ctgcatagcc cgcctgaccc ggatatcgtg atcgtcgata tcgacgaacg
     1321 cagcctggca ctgatggccg acagcgtcgg ccgctggccc tggccccgat ccatgcatgc
     1381 agaactcatc gaggggatcg ctcgccagca gcccaaggcc atcgtgttcg atatcctgtt
     1441 cagtgacccc gacctgacgc gaccacagga tgacgcttat tttgccgagg tcatcggtcg
     1501 cttcgatcag ctctatttcc ccatgctatt gctggatggc gccgatacgg gcaagggtat
     1561 cccgctgcgc gaatacgggt ccagactggg agcggtacca ggtccccaag ccgagccgca
     1621 ggccacagcg gccatggtgc tgcctttgcc tgccatattg gcgaccgggc gcgtcggtac
     1681 gcataacgcc atcgccgatc aggatggcgt cgtgcgcagt taccccatat atatgtatgc
     1741 aggtggctgg cggatccctt cgttgccggc gcgagtcgcc gtcgacctcg gctatgcgct
     1801 gccagccacg ggcgagatgc ctctgcactg gcatggcggc gtgctcgcgt atccccgtgt
     1861 ctcctacgcc gatctttacg ctgatctgca gcgacgtgtg tcgcagcgcc ccgcagcgga
     1921 attcaagggc aagatcgtga tcatcggcgc cacggccacc ggcttgcacg atgtgcgcgc
     1981 cacgccggtc gacggtttct atccgggtgt cgctatcctg gcgacggcgc tcgataacct
     2041 gaagaacgcc gactcgctga agcccgcgcc gctggcatgg gggttgggcg gcgggatgct
     2101 ggcgatcctg ctgctggccc tggccttcgc tgtacgtcgc catatcgcgg tcatcggcct
     2161 cgcgctcggc gccgcttccg cactggcgct gctgacggct tatcttgtcc tgcaggccca
     2221 atggcgggtc tgggtattga cgcccgtgct cttcgcctgg ctatatttcc tggttgccgc
     2281 tttcatggct tattggcagg agcgcaagac ccgcgagcgg gccatcgcca ccttcagtcg
     2341 cttcctcgat ccgcgggtgg tcgatgcgct ggtggcccag ggggtgaccc tggcatcgct
     2401 cggcggcaag agccgacagg tcagcgtgtt gttctcggac atccgcggtt tcaccacgct
     2461 ctctgaaggc agcacgccgg agcaggtggt cgaattgctg aatgcctact tcacactgca
     2521 ggccgaggcg gtatttcggc aagagggcac gctggacaag tacatcggcg atgccatcat
     2581 ggctttctgg ggtgcgcctg ccgagcagcc agaccatgcc ctgcgtgccg tggcggcggc
     2641 gttggacatg agcgacaagc tggagcagtt ccgcgcgcaa ctggtcgccc aagacaaacc
     2701 gctggggcag gcgctggaga tcggcatcgg cgtgcacagc ggtccggccg tggtgggctt
     2761 tatcggctca cctaacaagc aggaatacac cgccatcggc gatacggtga atctgaccag
     2821 ccgtatcgaa ggccagacca agggcatctg tcgcgtgctg gtgtcagaag atacgcgcca
     2881 gctctgcgag ttgcaggcca atggccattg cccgtatgat tttgtcgatc acggcgtgta
     2941 tgaagtcaaa ggcagggcga ggccggtgcg cctattcgag ccaaaaagaa aagcaggctg
     3001 atggaccgtc attcaagggg atgtgcatga aatctaggga tttgatcctg gtagggctgc
     3061 tgttgatgcc gccatgggtg ttggcaggcg agccagcgca gacgctggtg gctgccgacc
     3121 tcaagcgtga accatacgcc gatgctgcga ccatcctcag tttgccggcc gcaagtgcgg
     3181 tagaggtcat caagcgccag ggtggctgga tgcacgtgaa gtccgcggac ggcaaggaag
     3241 gctggctcaa gatggtcagt atcaagctcg gggcaggcgc tgcagccaag ggcgacaccg
     3301 gcctaggttc gctgcttaac gtcgcgcgca gcggacgctc gggcaacacg ggcgtcaccg
     3361 tcgccaccgg ggtgcgcggg ctcagcgcgg aagacctgaa gaacgccaaa cccgcgcccg
     3421 aagccttgaa gcgactcgac agcctcccca atggcaagcg tgaaggcgag tctttcgcca
     3481 gtgctggccg gctgcaagca cagaagatcg attatcttgg catgtcgcaa gccgccaccg
     3541 atcagggcgc caacaccttc ggcggagggc gctgaccatg aagcgagtcc tagtcctgat
     3601 tgttttatgc atctcagccg cgacggcgca agcctttgaa ctcaatatca acaagctggt
     3661 ggacgccatc ggcaaggtca aggatctgaa gccggtcgac gacaagggtg aagcggagat
     3721 cggtagcggc gtcgccgcca atctgctcgg cgccgcgccg ctggtgcagg acgacaagct
     3781 acaggcctat gtcagccgcc tgggctggtg gctggcgatg cagaccgaac gcgccgacct
     3841 gtcctggcgt tttggggtgc tcgacagcga taccgtcaac gccttcgccg cgcccggcgg
     3901 ctatgtcttc atcacgcgtg gtttgctgct gaggatgcgc aacgaagctg agctggctgg
     3961 tgtgctgtcg cacgagattg cccacgtgct aaagcgtcat cacctgaatg ccatccagaa
     4021 gaacgcgcgt acgggtctca tggccgatct tgcgagcatg gccgcaagca ctagcgagca
     4081 tgccgcgcta gcagagaagg ccattggcgc cggcacggaa ctctatacac gcggcctcga
     4141 caaggatgac gaattcgagg ccgaccgcat gggcgtggtc atcgccgcgc gcgcggggta
     4201 cgacccctat ggtctgcccg ccgttctaca gacgctggaa gctgccaatg cgcaggacag
     4261 caacctcgcg ctcatgttca agacccaccc tgcaccgcag cagcggctgg aattgctcga
     4321 tggcttgatg accggcaccc tcgatcggta tgcgagtcag cccgtggtcg cggaccgatt
     4381 catgaaggtc gtgggggatt acgccaagcg ttgaggcatc ggctggcgtc gagcgcgcat
     4441 gcccttaatc agcgcgaaaa gatacgccag gctggggtgc aggcaaggcc taggcctgga
     4501 ccgtctcggc ggcggtctcg ctgaatccag cgcttaattc ctgctgcatg aagctgtgga
     4561 acgcggcggc ggcttcggca tactcttcag tatcgaatgc atcctgcaag gcggcacgca
     4621 aggctttgcc cgatgccgtg agaaaggata gccggcgata gcctttggcg agcgccatct
     4681 cgttgtgcag caggccgcag gcatccgggc ccgcttgcca ggtttcgagc atggaagtgg
     4741 cgcggatgac ttcactctgg ccaaggtctg tcaggcggcc gagtttctgc agcgagatgc
     4801 cgtcctccat gcataccgcc aggaacacgc gaaagtgatc ggtctccagc gtcagggcca
     4861 atggcaggaa caggctcacc agggcttcgc gtgcggccag gtctgcattg tcatcgaaag
     4921 gcttgtgcat catgggctta tgccgcggcc agctcgtccg gatgcttctc cgagctgccc
     4981 gacgacttgc cgttgggcga ctggatggcg ctcaggcaca gggtgaccgg gatcgacttc
     5041 gaagtcggcg tgcgactgaa gtcgccgagg gcgccgatcg ggaccagcgg attggtttcc
     5101 gggtagtagc cggcgatgca gccctcgggg atgtcatatt ccaccaggcg gaactgctcg
     5161 gctttgcgct cgacgccgtc gtccgatacc gtgcgcagat ccacccactg atcggcctgc
     5221 atgcccagct tgtcgagatc cttgcggctg atgaagatga cgcgacgttg gccgaataca
     5281 ccacggtagc ggtcgtccag gccatagatg gtcgtgttgt actggtcgtg cgagcgcgtc
     5341 gtcatcaggt tgaacacggg cgcttcactc gtgcgcgtag cctgatggaa cggcagatcg
     5401 gtggggatgg cggccgtggt gaagttggct tttcccgttg cggtgcgcca gtcgtgattg
     5461 gcggcgctgt tgggcagata gaaaccgccg gggcgacgca cgcgcgtgtt gtaatcgtgg
     5521 aaaccgtcgt agaccttttc gatgaggtcg cggatgcggt cgtaatcggc gatcaggtcc
     5581 agccactgca cgcgcgagcc cgacagcgtg gcgtgtgcca tgcgggcgac gatcgccggt
     5641 tcggacagca ggtgcggcga agccgccttg ttgatgccgc cggagccgtg caccatgctc
     5701 atcgaatctt ccacggtcac catctgcgga ccgctggcct gcaggtcgat ctcggtacgg
     5761 cccaggcaag gcaggatcag tgcctgcttg cccatcacca ggtggctgcg gttgagtttc
     5821 gtgctgatgt tgaccatcag gtggcatcgt ctcagggcag cttcggtcag ggtggtatcg
     5881 ggcgttgcag ccgcgaagtt gccaccaagc ccgacgaaca cgcgaacgtc gccgcgctgc
     5941 atggcttcga tggtggcgac cacatccagg ccgttctcgc gcggcatctt ggcgccgaac
     6001 acctgttcca tccgatccag gaacgcctgc gaaggctttt cagtgatgcc gacggtgcgg
     6061 ttaccctgca cattgctgtg gccgcgcacc gggcaggcgc cggcgccctc tctcccgata
     6121 tgcccgccga gcaggagcag attgaccacc tgctgaatgg tgagcaccga atgcttgtgc
     6181 tgtgtcaggc ccatgcccca ggtgcagatg acgcgaggcg aagcgaggta gatattggcg
     6241 gcggcctcga tctcggcacg tgagatcccg gattcctcca ggatcacatc ccagcgctcg
     6301 gtgcggatgt cggcgaggaa agactcgaag ccgtgcgtgt ggcgatcgat gaacgcctgg
     6361 tcgatgacac ctggctggcc gttcgccagc gccgcgtcat gctgggcgat gatgtgcttg
     6421 agcatgccct tgatgacggc gagatcgcct ccgatattgg gctgcaggta gagtgagctg
     6481 atcggcgtgc tgccgccggt cagcatctcc accgggcttt gcgggaaagc gaacttttcc
     6541 aggccccgct ctttcagtgg gttgatggaa acgatcttgc agccgcgctt cgccgcgcta
     6601 cgcaactcgc ccagcatgcg tggatggttg gtgcccggat tctggccgaa gatgaaaatg
     6661 gcgtccgcct tgtggaaatc ctccagcttg acggtcccct tgccgatgcc gatggactcg
     6721 atcagcgcat agccgctggc ctcgtggcac atgttggagc agtcggggaa gttgttggtg
     6781 ccgaattcgc gcacgaacag ctggtacagg aacgccgctt cgttgcttgc acggcctgag
     6841 gtatagaagg cggcctggtc cggatggtcc agcgcctgaa gggcactggc gattttttgg
     6901 aacgcgttgt cccagctgat ggcctcgtag tggtccgtag cggcgttgta gaccatgggg
     6961 tgggtgagac gcccctggtt ctcgagccag aaatcggatt gcgccaatag tgacgtcacc
     7021 gagtgcttgg cgaagaatgc cggggtgacg cgcctggaag tcgcttcggc agcgacggcc
     7081 ttgacgccgt tttcgcagaa ttcgaaggaa gaggcatgtt ccgg
//
LOCUS       DATMTW010000061         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_541987, whole genome shotgun sequence.
ACCESSION   DATMTW010000061 DATMTW010000000
VERSION     DATMTW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541987"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1012)
                     /gene="cyoE"
                     /locus_tag="VN084_01225"
     CDS             complement(107..1012)
                     /gene="cyoE"
                     /locus_tag="VN084_01225"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWT28104.1"
                     /translation="MSAVSTLKLQRVSLGCRNFLALCKPRVTALIVFTAMIGMFLATP
                     DVLPLGLLLAATIGIGMASGAAAAFNCLIEQTIDARMARTRGRPLPTGMVTSTQTVIF
                     ATLTGATGLLILYVFVNPLTMWLTLATFVGYAVIYTVFLKPATPLNIVIGGASGAMPP
                     ILGWAAVNNVVAPEALILFLIIFAWTPPHFWALALYRRAEYAKVGMPMLPVTHGEEFT
                     RLHILLYTIILFAVALMPYGHGMSGVIYLVASLILNAGFMAYAIGLYRQYSDQLARRT
                     FRYSILYLTLLFAALLVDHYWRITL"
     gene            complement(1022..2026)
                     /locus_tag="VN084_01230"
     CDS             complement(1022..2026)
                     /locus_tag="VN084_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWT28105.1"
                     /translation="MRTDRSAFGFRQLVIITSLLAFCVVGLGAFVRLSDAGLGCPDWP
                     GCYGSMTVPQSEAAIQQAQTAYPQSTVEVHKAWKEMLHRYLAGTLGILILGLCVLGWH
                     GRKVLRVSPWVPTALVLLVGLQAMLGMWTVTLLLKPVIVSAHLLGGMSTLALLVWIAH
                     RHWGVASQRFLTSPWLRFWVRGAIVLIFAQIFLGGWTSSNYAALACMDFPTCHGELVP
                     PMDFGVAFHMIRDLGQGPDGSPLSLPALVAIQWVHRLGAIVVFLYLGVLAWRLSRYPQ
                     LRPFAQALFLLLLMQISLGIGNLLLHLPLVLAVAHNLGAALLLITIVILNSKITELPK
                     "
     gene            complement(2040..2558)
                     /locus_tag="VN084_01235"
     CDS             complement(2040..2558)
                     /locus_tag="VN084_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28106.1"
                     /translation="MPIIIVVLMYLFEWHPQGASQGELLAPPRPILLSGDLRDANAAP
                     LPADLLRDKWSMVYVTVQCEAQCLQRLHTMRQLHASMAKDSARLQRILVAQDADWQRL
                     QADYPDMVIINQPRAAIVSLSQDLQVMPMAESRIYLIDPLANLMMSFPESVAPKAVRK
                     DLVRLLKAAWAG"
     gene            complement(2640..>3222)
                     /locus_tag="VN084_01240"
     CDS             complement(2640..>3222)
                     /locus_tag="VN084_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWT28107.1"
                     /translation="LHAPAVPLPVTMDDATAWRYHRVRVTGVYDASHQILLDNQVYQD
                     RAGYHVLTPLRTAGNRLVLINRGWVPGAADHTRLPQTTVPTGEQTITGQVWIPPSKFF
                     SLESTAPQDSPSAWTPVWQNLDMARYAGIVQQPVSPIIIRLDQDAAGGYVRDWPRPAE
                     RIETHIGYAYQWFGFAVAAFCIYLYVGFRKRPA"
BASE COUNT          765 a          933 c          949 g          575 t
ORIGIN      
        1 acctagggct ggcctgcata aaggccagca aaaaaaagcc aggtttcaac ctggcttttt
       61 ttatgggcac gcttcgatgc gtgcgccgga gatgccgatg ttcgacctag agcgtgatgc
      121 gccagtaatg atcgaccagc aaggccgcaa acagcaaggt gagatagagg atcgaatagc
      181 ggaaagtgcg gcgcgccaat tgatctgaat actgacgata aagtccgatg gcataagcca
      241 tgaaaccagc attcagtatc aatgaggcca caaggtagat caccccgctc atgccatggc
      301 cgtaaggcat cagcgcaaca gcgaacaaga tgatggtgta gagcaggatg tgtagccggg
      361 tgaactcctc gccgtgggtc acaggcagca ttggcatacc caccttggcg tattcagctc
      421 gccgatagag cgccagcgcc cagaaatgcg gcggggtcca ggcaaagatg atcaggaaca
      481 ggatcagcgc ttcaggggca accacattat tgaccgcagc ccagcccagg ataggcggca
      541 tggcaccgga ggcaccgccg atgacgatgt tgagcggcgt cgcgggtttg aggaaaacgg
      601 tatagatcac cgcatagccg acaaaggtcg cgagcgtgag ccacatggtc agcggattga
      661 cgaagacata caggatcaat aagccggtag cgcctgtcag cgtcgcaaag atcacagtct
      721 gggtggacgt taccataccg gtcggcaacg gacgtccacg ggtcctcgcc atgcgcgcgt
      781 cgatcgtttg ctcgatcagg cagttgaacg ccgctgcggc tccggaggcc atgccgatcc
      841 cgatggtcgc cgccagcaac aggcccagcg gcagaacatc cggcgtcgcg aggaacatgc
      901 cgatcatcgc ggtgaatacg atgagcgccg tgacacgggg cttgcataac gccaggaagt
      961 tacgacaccc caggcttact cgttgcagtt tcaaggtact gacagcgctc atgtaacctc
     1021 cctattttgg taattctgtt atttttgaat tcagtattac gatagtgatc aacaacaagg
     1081 cagcacctag attgtgcgcc accgcaagca ctaggggaag atgcagcaga aggtttccga
     1141 tccccaggct gatttgcatc agcagtagca aaaacagcgc ctgtgcgaac ggccgcaact
     1201 gtggataacg agacaagcgc caggccagca cacccagata aaggaacaca acgatcgccc
     1261 ctaggcgatg gacccattgg atcgccacca gcgcaggcaa gctcagcggc gaaccgtccg
     1321 ggccctggcc caggtcacgg atcatatgga aagcgacgcc gaaatccata ggcggcacaa
     1381 gctcgccgtg gcaggtcggg aaatccatgc aggccaaggc tgcataattg gaactggtcc
     1441 agccgcccag gaatatctgg gcgaagataa gcacgatcgc accccgcacc cagaaccgca
     1501 gccatggact ggtcaggaag cgctgtgagg cgacccccca gtgacgatgt gcgatccata
     1561 ccagcaaggc tagcgtgctc atgccgccaa gaagatgcgc cgacacgatg acaggtttga
     1621 gcagcagcgt cacagtccac atgccgagca tggcctgcag accgaccagc aacaccagcg
     1681 ctgtaggtac ccaaggcgaa acgcggagga cttttcggcc atgccaccct aacacgcaca
     1741 atccgaggat caatatcccg agcgtgccgg ccaggtagcg atgtagcatc tctttccagg
     1801 ccttgtgcac ttccacggtc gattgcggat aagcggtttg cgcctgctgg atagccgcct
     1861 cgctttgcgg cacggtcatg ctgccgtagc agcccggcca atccggacag ccgagacccg
     1921 cgtccgaaag gcggacgaac gcgcccagac caaccacgca aaaagcgagc aggctggtga
     1981 tgatcaccag ttgtctgaag ccgaaggcgc tacggtccgt tctcactggt ccactccgct
     2041 catcctgccc acgccgcttt cagtaaccgc accagatcct tgcgcacggc cttaggggca
     2101 acggactccg gaaagctcat catcagattc gccaatggat caatgagata gatgcgggac
     2161 tcagccatgg gcatcacttg cagatcctga ctcagcgaaa cgatagctgc gcgcggctga
     2221 ttgatgatga ccatgtccgg atagtcggct tgcagacgct gccagtcagc gtcctgtgcg
     2281 acgaggatgc gttgcaggcg cgcgctgtcc ttggccatcg aggcatgcag ctgtcgcatc
     2341 gtgtgcaatc gttgcaggca ctgcgcttcg cattgcacgg tgacatagac catgctccat
     2401 ttatcacgca acagatccgc cgggagcggc gccgcattgg cgtctcgcag gtcgccggaa
     2461 agaaggatcg gtcgcggcgg cgcaagcaac tcgccttgac tcgcgccttg cggatgccat
     2521 tcaaagagat acatcagaac cacgatgatg ataggcaagg cgaaaaagaa cgctagcagg
     2581 cagaacaccc accgacctct cgcttgccgt tgctctgcag ttaaattgac gctggacatt
     2641 taggcaggtc ttttcctgaa accgacatag agatagatgc aaaacgccgc cactgcaaaa
     2701 ccgaaccact ggtaagcgta tccgatatgg gtttcgatgc gctcggcagg tcgcggccaa
     2761 tcccggacat agccgccagc cgcatcctga tcgagccgga tgatgatcgg cgacacaggc
     2821 tgttgcacga taccggcata acgtgccatg tcgaggttct gccagaccgg tgtccatgcg
     2881 gatggcgagt cttgtggagc cgtgctctcc agtgagaaga acttgctcgg cgggatccat
     2941 acctggccag tgatcgtctg ctcgccggtt ggcactgtgg tttgtggcaa tcgcgtgtga
     3001 tctgccgcgc cggggaccca gccgcgattg atcagcacca gtcggttacc tgccgtgcgc
     3061 aggggtgtca acacatggta gccagcacga tcctgatata cctggttgtc gagcaggatc
     3121 tgatggctgg cgtcgtagac gccggtgact cgtacccggt gatagcgcca tgcagttgcg
     3181 tcatccatgg ttaccggcaa cgggactgct ggtgcatgca ga
//
LOCUS       DATMTW010000062         7126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_340674, whole genome shotgun sequence.
ACCESSION   DATMTW010000062 DATMTW010000000
VERSION     DATMTW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340674"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VN084_01245"
     CDS             complement(<1..454)
                     /locus_tag="VN084_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWT28108.1"
                     /translation="MLVGLTELSFAGPRETQTTIEEPSVSAAEPEPETIQVQILDKRI
                     SIEDRLRLRRDLYEYSKSVDAAHIQIEERRRLMRKRIQERFLDADHDNDGSLSREEAT
                     EMLPQVARHFNQFDLNADGVLTLSELEDAQAKAAERQRALTAKIDGPNP"
     gene            919..1635
                     /gene="ompR"
                     /locus_tag="VN084_01250"
     CDS             919..1635
                     /gene="ompR"
                     /locus_tag="VN084_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HWT28109.1"
                     /translation="MKKILMVDDDLRMRELLQRYLGEQGFTVKTVSDATEMDIALTHE
                     HVDLLVLDLMLPGEDGLAICRRLRAAGNTMPIIMLTARGDEVDRIVGLEMGADDYLPK
                     PFNPRELLARINAVMRRHGPTVPSAPAIEGDVISFGDLTFDPSTRSLLRNGVAITITS
                     GEFALLKVFVDHPRQPLSRDRLMQLARGRELDVFDRSIDVQVSRLRKLIEPDPAHPRY
                     LQTMWGFGYVFIPDGEALKT"
     gene            1645..2952
                     /locus_tag="VN084_01255"
     CDS             1645..2952
                     /locus_tag="VN084_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28110.1"
                     /translation="MKFLPRSLLAHVMLLIALLLAVGQYAALKLFDYFEREPRAMAAA
                     LQAITVVNYTRASLLASHENRRLALLSELSGKEGVRVYAADLLEEIEPLPDDPFIRMI
                     AAKIRDRLGKDTLVSYNHFGIPGLWVSFSIEQDDFWVVIPRIQVDRPFPWQWLGWGAL
                     MILLSLMGAYVIAARINRPLSLLVKAADRLRNGEQPPKLPEVGVAELREVSHTFNQMS
                     EALHRLDTERTLLLAGISHDLRTPLARLRLAVEMLPEDQCGSFKTGMVQDITDMDDII
                     HQFLDFVRGVEGEPTQMVDLNELLKSIVERNQRAGRDVILRLSAQHLIPLRPLAMQRL
                     IGNLIDNAYKYGHGEVIVSTRINSGSVVVSVMDNGPGIPASQMSRLLRPFERLDKARG
                     NEGGSGLGLAIAERIAKLHKGELTLLNRPQGGLEARLTLPIVV"
     gene            complement(3072..4460)
                     /gene="cysG"
                     /locus_tag="VN084_01260"
     CDS             complement(3072..4460)
                     /gene="cysG"
                     /locus_tag="VN084_01260"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HWT28111.1"
                     /translation="MDALPIFMRIQGRRCVVIGGGEVAARKVTMLLKAGGDVVVISPE
                     LHHELAEMHAAGKITHLADVFRAGHLADACLAVAATDEADVNRAVFDEANARQLPVNV
                     VDCPELCTFTMPSIVERYPITIAISSGGAAPVLARMLRARIETMLPASYGRLAGLASE
                     FRDRVKARFATTQERRIFWERVFQGPIGERVLSGQEPAARAALEQAIASDQAGQDHGE
                     VYLVGGGPGDPDLLTFRALRLMQQADVCVYDKLVSREVMELVRRDAELIYVGKARDQH
                     TLPQEEINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLMQHGVPFQVVPGITAA
                     NGVSAYAGIPLTHRDYAQSCVFTTGHLKDGTVDLDWPALVRPNQTVVIYMGLVGLDEI
                     CRQLVSHGLDATTPAAVVQQGTTPRQKVVVADLATLAAQVQAADMKPPCLTIVGEVVK
                     LREKLAWFDPQA"
     gene            4624..5958
                     /gene="miaB"
                     /locus_tag="VN084_01265"
     CDS             4624..5958
                     /gene="miaB"
                     /locus_tag="VN084_01265"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWT28112.1"
                     /translation="MSTPRKVFIKTFGCQMNEYDSSRMADMLHSSEGMLTTDNVDEAD
                     VILLNTCSIREKSEDKVFSHLGRFIPLKEKNPNLIIGVGGCVASQEGEQIVARAPYVD
                     VVFGPQTLHRLPEMISHRRRTGISQVDISFPEIEKFDHLPPPRVEGAAAFLSIMEGCS
                     KYCTFCVVPFTRGDEVSRPFGDILVEAVQLAEQGVKEITLLGQNVNSYRGESPTGAPA
                     DLALLIEYIAEIPQIERIRFTTSHPNEMSDALIDCFVRIPKLVSHLHLPVQAGSDRIL
                     MAMKRNYTALQYKSIIRKLRKARPDICISSDFIVGFPGETEADFEATMKLLKDLDFDF
                     SFSFVYSARPGTPASYLPDDTPQRVKLERLARLQAVNEEQGLSISRSMVGTRQRVLVE
                     SLSKKDTGELAGRTDNNRIVNFPGGAELINQFVEVRITDAQPHTLRGELIRA"
     gene            5961..6926
                     /locus_tag="VN084_01270"
     CDS             5961..6926
                     /locus_tag="VN084_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWT28113.1"
                     /translation="MEITLSPEDNTRLANLCGVLDENLKQIESALDVGISRRGSHIRL
                     SGEAHNMRLAAQLLENFYERAKKPISLEEVQLSLVEVDRLKPEEVTTAMPVLMTRRQD
                     LHGRTPRQVEYLQQIQEFDITFGIGPAGTGKTYLAVASAVDALTRDRVKRIVLVRPAV
                     EAGERLGFLPGDLSQKVDPYLRPLYDALYDLAGYDTVNKMFERGAIEVAPLAYMRGRT
                     LNQSFIILDEAQNTTPEQMKMFLTRIGFGTKAVITGDVTQIDLARGQKSGLVEAQKIL
                     KEVRGIAFTKFLSDDVVRHPLVQKIINAYEQYQEAQEQSRKPEAS"
     gene            6926..>7126
                     /locus_tag="VN084_01275"
     CDS             6926..>7126
                     /locus_tag="VN084_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HWT28114.1"
                     /translation="MPTLNYQVQYATDDPQLPTAAQFAKWARKALRVDTDVTLRIVGE
                     NEGRALNHDYRGNDYATNVLTFP"
BASE COUNT         1426 a         2268 c         2049 g         1383 t
ORIGIN      
        1 ccgggttcgg gccatcgatc ttggctgtca acgcgcgttg acgttccgcc gccttggcct
       61 gggcatcctc cagctcggac aaggtgagca cgccatcggc attgaggtcg aactggttga
      121 agtgacgggc gacctgcggc aacatttcag tcgcctcttc gcgtgagaga gagccgtcgt
      181 tatcgtgatc ggcatcgagg aagcgctcct gaatgcgctt gcgcatcagc cgacggcgct
      241 cttcgatctg gatgtgggcg gcgtctaccg atttggaata ttcataaagg tcgcgacgta
      301 agcgcagtcg gtcttcgatg ctgatgcgct tgtccaggat ctgcacctgg atggtttctg
      361 gttctggttc tgcggcggag acgctaggtt cttcgatcgt ggtctgcgtt tcgcgtgggc
      421 cggcaaaaga caactcggtc agtccgacca gcacaaaaag cgcaatgaga gcgtgcttgt
      481 gatatggcgc ctgaaaatcc gtgatcagca tacggaatac accaccaaaa gttgagcttt
      541 cagcatcgtt gctttccatc ccccagccag tgtttttgcg atgaccactc cagctattac
      601 gagtgggatc gtcatttgtc taggatgcgc aatgcaatgg atttgttcat tactttgcgt
      661 tccagccagc atatttttgt cgtatatcga tcgctgttcc acccgcgaga accgctttgc
      721 gatccgctgt tccgtcgtcg ggccaagcca ttttgcatat ggcaagagtc cgcaatcatg
      781 acgcatgaat gtaaatcgaa tgtttcatcg actcccttga ttgtaaaaaa ttgtaactta
      841 atcaagtgtt atcaaaaact taacgtaggg ctttcaattc cgggccattc cgcagaaaat
      901 acgggcatgc aacccccgat gaaaaagatc ctgatggttg acgacgactt gcgcatgcgc
      961 gagttgctgc aacgctatct cggtgaacaa ggcttcaccg tcaagactgt ttccgacgcg
     1021 accgaaatgg acatcgcgct cacgcacgaa catgtcgacc tgctcgtact cgatctcatg
     1081 ctgccgggag aagacggcct ggcgatctgc cgacgattgc gggcggccgg caataccatg
     1141 cccatcatca tgctgactgc gcgtggcgat gaagtcgatc gcatcgtcgg gctggagatg
     1201 ggcgctgatg attacttgcc caaacccttc aacccacgcg agctgctggc gcgcatcaac
     1261 gccgtcatgc gtcgacatgg tccgaccgta cccagcgcgc cggcgatcga gggcgacgtc
     1321 ataagcttcg gcgacctgac cttcgatcct tccacacgca gcctgttgcg caacggggtc
     1381 gccatcacca tcaccagcgg cgagttcgcc ctgctcaaag tctttgtcga tcatccacgg
     1441 caaccgctgt cccgggatcg gctcatgcaa ctggcgcggg gccgcgagct cgatgtgttc
     1501 gaccgcagca tcgacgtcca ggtttcgcgc ctgcgcaagc tgatcgaacc cgaccctgcg
     1561 catccgcgct atctgcagac catgtgggga ttcggctatg tcttcatccc ggatggcgaa
     1621 gcgctcaaaa cctagcgtgt acttgtgaaa ttcctgccgc gttcattgct ggcgcacgta
     1681 atgttgctga tcgccttgct gctggcggtc ggccagtatg cggcgctcaa gctgttcgac
     1741 tatttcgagc gcgagccgcg cgccatggcc gcggccctgc aagccatcac cgtcgtcaat
     1801 tacacccgcg cctctctact ggcatctcac gagaaccgtc ggctcgccct gctctccgaa
     1861 ctctccggca aggagggtgt acgcgtctac gcggccgacc tgctggaaga gatcgaaccc
     1921 ctgccggacg accctttcat ccgcatgatc gccgccaaga tccgtgaccg gcttggcaag
     1981 gacaccctag tctcgtataa ccatttcggc atccctggcc tctgggtgag tttcagtatc
     2041 gagcaggacg atttctgggt ggtgatcccg cgtatccagg tcgatcggcc gttcccctgg
     2101 cagtggctgg gctggggggc cctgatgatc ctgctctcgc tgatgggggc ctacgtgatc
     2161 gctgcgcgca tcaaccgtcc gctgagcctg ctggtgaagg ccgctgaccg actgcgtaac
     2221 ggcgagcaac cgcccaagct gccggaagtg ggggtcgccg aactacgcga ggtgagccat
     2281 acgttcaacc agatgtcgga agccctgcat cggctggata ccgaacgtac gctattgctg
     2341 gccggcatct cgcatgactt gcgcacgccg ctggctcggc tgcggcttgc cgtcgagatg
     2401 ctgcccgaag accagtgcgg cagtttcaag accggcatgg tgcaggacat caccgacatg
     2461 gacgacatca tccaccagtt cctcgatttc gtccgtggcg tcgagggcga gccgacacag
     2521 atggtcgacc tcaacgaact gctgaaatcg atcgtggaac gcaatcagcg tgccggcagg
     2581 gacgtcatcc tgcggctatc ggcacagcat ctcatcccgt tgcggcctct ggccatgcag
     2641 cgcctgatcg gcaacctgat cgacaacgcc tacaaatacg gtcatggtga agtgattgtc
     2701 agcacacgca tcaactccgg atcagtggtg gtgagcgtga tggacaacgg gccagggatc
     2761 cccgcatcgc agatgtcacg cctgctgcgc ccgttcgaac gtctggacaa ggcgcgcggc
     2821 aatgaaggcg gcagcgggct tgggctggcc atcgccgaac gtatcgcgaa gttgcacaag
     2881 ggtgagttga cgctgctcaa tcgcccgcaa ggcggattgg aagcgcggct gacactgcct
     2941 atcgtggtct gatcgcagta cccttgggcg ctgcgcactg cgttgacggt gcgtgcgggt
     3001 ggcgcgaacc ccagggtgcc agttggtgtt gaatgaaagc gcggcccggg aatccagcct
     3061 gcgcatgctg gctatgcctg cggatcgaac caggccagct tctcgcgcag cttgaccact
     3121 tcaccgacga tggtcaggca aggtggtttc atgtctgcgg cctgcacctg cgccgccagt
     3181 gtcgcaaggt cggccaccac cactttctgc cgcggcgtcg tcccctgctg caccacggct
     3241 gcgggtgtcg tggcgtccag gccatggctg accagctgcc ggcatatctc atccagcccg
     3301 accagcccca tgtagataac taccgtctga ttgggtcgca ccaatgccgg ccagtcgaga
     3361 tccaccgtgc cgtccttgag atgcccggtg gtgaaaacgc aggattgcgc ataatcgcga
     3421 tgcgtgagcg gaatcccggc atacgccgag acgccgttgg cggcggtgat gcccggcacc
     3481 acctggaagg gtacgccatg ctgcatgagg gtttcgatct cctcgccgcc acggccgaag
     3541 atgaacgggt ctccgccttt caggcgaagt acgcgcttac cctccttggc aagtcttacc
     3601 agcagctgat tgatctcttc ctgcggcaag gtatgctggt cacgcgcctt gccgacatag
     3661 atcagctcgg cgtcgcgccg caccagttcc atgacctctc tgctcaccag tttgtcgtag
     3721 acgcagacat cggcttgctg catcaggcgc aaggcacgga acgtcagcag atccggatcg
     3781 cccggtccgc caccgaccag atagacctcg ccgtgatcct gcccagcctg gtcgctggca
     3841 atcgcctgct cgagcgctgc gcgcgctgcc ggctcctgcc ccgagagtac gcgctcaccg
     3901 atcggcccct ggaacacacg ttcccagaag atgcgccgct cctgcgtcgt cgcgaagcgc
     3961 gccttcacgc ggtcgcggaa ctcgcttgcc agacccgcca gccggccata actcgcaggc
     4021 agcatggtct cgatccgtgc gcgcagcatc cgcgccagca ccggggcggc gccaccgctg
     4081 gaaatagcga tggtgatcgg atatcgctcg acgatggaag gcatggtgaa cgtgcatagc
     4141 tcggggcagt cgaccacgtt cactgggagc tgccgagcat tggcctcgtc gaagacggct
     4201 cgattgacat ctgcttcatc agtcgccgcc acggccaggc aggcatccgc cagatggccg
     4261 gcacgaaaga cgtctgccag gtgcgtgatc ttgccggcgg cgtgcatctc agcaagttca
     4321 tgatgtaatt caggtgagat gaccaccaca tcgccgcccg ctttcagcag catggtgacc
     4381 ttgcgcgcag cgacttcgcc gccgccgatg acgacgcagc gacggccctg tatccgcatg
     4441 aaaatcggta atgcatccat ggcgccattt tacgctggtt tgtgcatcgg catgggctgc
     4501 gctacaatca ggccttagct tactgttttt ttaagatatt ttgcatcctc ccccagtcag
     4561 tttcaccgcc agttccatct cgatctgcgc tgtgctcccg aaccgacacg ccgccaagcc
     4621 cgcatgagca cgcccaggaa agttttcatc aagaccttcg gctgccagat gaacgaatac
     4681 gattcgtcac ggatggccga tatgctgcac agcagcgaag gcatgctgac gaccgacaac
     4741 gtcgacgaag ctgacgtcat cctgctcaac acctgctcca tacgtgaaaa gtccgaagac
     4801 aaggtgttca gccatctcgg tcgcttcatc ccgctcaagg aaaagaaccc gaacctgatt
     4861 atcggcgtcg gcggctgcgt cgccagccag gagggcgagc agatcgtcgc ccgtgcgcct
     4921 tatgtcgatg tcgtcttcgg gccgcagacc ttgcaccgtt tgccggagat gatcagtcac
     4981 cggcgccgca ccggcatctc gcaggtcgac atcagcttcc ccgagatcga gaagttcgat
     5041 catctgccgc cgccgcgcgt cgaaggcgcg gccgcgttcc tctccatcat ggaaggttgt
     5101 agcaagtact gcaccttttg cgtggtgccg ttcacccgcg gcgacgaagt ctcgcgtccg
     5161 ttcggcgaca tcctggtcga agcagtgcaa ctggcggagc aaggcgtgaa ggagatcacc
     5221 ctgctaggac agaacgtcaa cagctaccgc ggcgaatcac cgaccggcgc gcctgccgat
     5281 ctggcgttgc tcatcgaata catcgcagag atcccgcaga tcgaacgcat ccgcttcacc
     5341 acgtcacatc cgaacgagat gagcgacgcg ctgatcgact gcttcgtgcg catccccaag
     5401 ctggtctcac acctgcacct gccagtgcag gcgggttccg accgcatcct catggcgatg
     5461 aagcgtaact acaccgcatt gcagtacaaa tccatcattc gcaagctgcg caaggcgcgc
     5521 cccgacatct gcatcagctc ggatttcatc gtcggtttcc cgggcgagac cgaggcagac
     5581 ttcgaagcga cgatgaaact gctgaaggat ctcgatttcg acttcagttt cagtttcgtc
     5641 tatagcgctc ggccaggtac gccggcttcc tacttgcccg acgacacccc gcaacgcgtc
     5701 aagctcgagc ggttggcgcg gctgcaggcg gtcaacgagg aacaaggcct gtccatcagc
     5761 cgcagcatgg tcggcacgcg ccagcgcgtg ctggtggaga gcctgtcgaa aaaggatacc
     5821 ggcgaactcg ccggccgcac cgacaacaac cgtatcgtca atttccctgg cggcgcggaa
     5881 ctcatcaacc agttcgtcga agtccgcatc accgacgccc agccgcatac cttgcgcggc
     5941 gaacttatca gagcctaatc ttggaaatca ccttaagccc cgaagacaat acccgcctcg
     6001 ccaacctttg cggcgtgctg gacgagaacc tgaagcagat cgaatccgcg ttggacgtcg
     6061 gcatctcacg ccgtggctcg catatccgcc tgtcgggcga agcccacaac atgcggcttg
     6121 ccgcccagtt actggaaaac ttctacgaac gcgcgaagaa acccatcagc ctcgaagagg
     6181 tccagctgag cctggtcgag gtcgaccgcc tcaagcccga agaagtgacg acggcgatgc
     6241 cggtattgat gacccgccgc caggacctcc atggacgcac accgcgccag gtggaatacc
     6301 tgcagcagat ccaggagttc gatatcacct tcggcatcgg cccagccggt accggcaaga
     6361 cctatctcgc agtggccagc gccgttgacg cgttgacgcg tgaccgcgtg aaacgcatcg
     6421 tgctggttcg tcccgcagtc gaagccggcg agcgcctggg cttcctgccc ggcgatcttt
     6481 cgcaaaaggt cgacccttac ctgcgcccgc tgtatgacgc gctctacgac ctcgccgggt
     6541 acgacaccgt caacaagatg tttgagcgcg gcgccatcga agtcgcgccc ctggcttaca
     6601 tgcgcggtcg cacgctgaac cagagcttca tcatcctcga cgaggcgcag aacacgacgc
     6661 cggaacagat gaagatgttc ctcactcgga tcggtttcgg caccaaggcc gtgatcaccg
     6721 gcgacgtcac gcagatcgac ctcgcccgcg gccagaagag cggcctggtc gaagcccaga
     6781 agatattgaa ggaggtgcgt ggtatcgcgt tcaccaagtt cctgtccgat gacgtcgtgc
     6841 gccatccgct cgtgcagaag atcatcaacg cctacgagca ataccaggaa gcacaggaac
     6901 agtcgcgcaa gccggaagcc tcgtgatgcc aacgctcaac taccaggtgc aatacgccac
     6961 cgacgacccg caactgccca ctgccgcgca attcgccaaa tgggcgcgca aggccctgcg
     7021 ggtggacacc gacgtcaccc tgcgcatcgt cggcgaaaac gaaggccgcg cgctcaatca
     7081 cgactaccgt ggcaatgatt acgcgaccaa tgtactcacc ttcccc
//
LOCUS       DATMTW010000063         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_173204, whole genome shotgun sequence.
ACCESSION   DATMTW010000063 DATMTW010000000
VERSION     DATMTW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173204"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VN084_01280"
     CDS             <1..1726
                     /locus_tag="VN084_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWT28115.1"
                     /translation="AGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAAL
                     ERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLP
                     DKAIDLIDEAASRIKMEIDSKPESMDKLERRLIQLKIEREAVKKEKDEASKKRFGLIE
                     EEIARLEKEYADLEQIWTSEKAQVQGGQHIKEAIEKLKLQMEDAKRKGEWQKVSELQY
                     GKLPQLEAQLKEATANEGEPNKMKLLRTQVGAEEIAEVVSRATGIPVSKMMTGEREKL
                     LHMEDKLHERVVGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELTKA
                     LAGFLFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVIL
                     LDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIIMTSNLGSQMIQSMAGDDY
                     QVIKLAVMGEVKANFRPEFVNRIDEVVVFHALGEAHVKSIASIQLQNLAKRLAGMEMT
                     LDVTDAALAEIANAGFDPVYGARPLKRAIQSEIENPLAREILQGNFAAKDTIRVDYKA
                     NKMVFSKA"
     gene            1763..>2463
                     /locus_tag="VN084_01285"
     CDS             1763..>2463
                     /locus_tag="VN084_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWT28116.1"
                     /translation="MPLKLVMMCGLWMLAMQAVPAWAAFSAREQATFENEPPQIRALL
                     KQALTYERSANEYDGEWQAANLYCRASRLGSPEAEYRLGMLYAFGKGVPKNRGLAASL
                     FSLASSQGHFEAQKMLETINYTTSDLPACVLQAVAPEKALAAIDVQIDNLPENKRWVV
                     DLVSTMSDWYDIDPKLILSIIQVESNFKVGAQSNKAAQGLMQLIPATAERFNVKNAYD
                     AAQNIKGGIAYIRWL"
BASE COUNT          540 a          734 c          778 g          411 t
ORIGIN      
        1 cgccggcaag gccgagggcg ccatggatgc cggcaacatg ctgaaacctg cgctggcgcg
       61 cggcgagctg cactgcgtcg gcgccactac gctggacgaa taccgcaagt acatcgagaa
      121 ggacgccgcg ctcgaacgcc gcttccagaa ggtgctggtg gacgagccta gcgtcgaggc
      181 caccatcgcc atcctgcgcg gcttgcagga gaaatacgaa ctgcatcatg gcgtcgacat
      241 caccgacccg gccatcgtcg ctgcggccga gttgtcgcat cgttacatca ccgaccgctt
      301 cctgccggac aaggccatcg acctcatcga cgaggccgct tcgcgcatca agatggagat
      361 cgattccaaa ccggaatcga tggataaact cgagcgccgc ctgatccagc tcaagatcga
      421 gcgcgaagcc gtcaagaagg aaaaggacga ggcctccaag aagcgcttcg gcctgatcga
      481 ggaagagatc gcgcgcctgg agaaggaata cgccgacctc gaacagatct ggacttcgga
      541 aaaggcccag gtacaggggg gccagcacat caaggaagcc atcgagaagc tcaagttgca
      601 gatggaggat gccaagcgca agggcgaatg gcagaaggtc tccgagctgc agtacggcaa
      661 gttgccgcaa ctcgaggcgc agctgaagga ggcgacggcc aacgaaggcg agccgaacaa
      721 gatgaagctg ctgcgtacgc aggtcggcgc cgaagagatt gccgaagtgg tgtcgcgcgc
      781 gaccggcatc ccggtctcca agatgatgac cggcgaacgc gagaagctgc tgcacatgga
      841 agacaagctc cacgaacgcg tggtcgggca ggacgaggct gtgcgcctgg tgtctgacgc
      901 catccgccgt tcgcgttccg ggctttccga ccccaaccgg ccatatggtt cgttcctgtt
      961 ccttggtcct acgggcgtcg gcaagaccga actgaccaag gcgctggcgg gcttcctgtt
     1021 cgattccgaa gaccacctga tccgcatcga catgagcgaa ttcatggaga agcattccgt
     1081 cgcccgtctg atcggcgcgc cgccgggtta tgtcggttac gaggagggcg gttacctgac
     1141 cgaggccgtg cgccgcaagc cttactcggt gatcctgctc gacgaggtcg agaaggcgca
     1201 tccggacgtg ttcaacgtgc tgctgcaggt gctggacgac ggtcgcctga ccgatggcca
     1261 gggccgtacc gtggatttca agaacaccgt catcatcatg acttccaacc tcggttcgca
     1321 gatgatccag agcatggccg gcgacgacta ccaggtcatc aagctcgcgg tgatgggcga
     1381 ggtgaaagcc aacttccgtc cggaattcgt caatcgtatc gacgaagtcg tggtgttcca
     1441 tgccctgggc gaagcccatg tgaagtcgat cgccagcatc cagctgcaga acctggccaa
     1501 acgcctggcg gggatggaga tgacgctcga cgtcaccgac gcggcactgg ccgagatcgc
     1561 caatgccggc ttcgatccgg tttacggcgc gcggcctctg aagcgggcga tccagtccga
     1621 gatcgagaac cccttggcgc gggagatcct gcaggggaat tttgcggcca aggatacgat
     1681 cagagtagac tacaaggcca acaaaatggt ctttagcaag gcctgaggcg ccgctgcggc
     1741 ggtagcccgg agataaggga cgatgccgtt gaagttagtc atgatgtgcg ggttgtggat
     1801 gctggccatg caggcggtgc ctgcatgggc ggcgttctcg gccagggaac aggcgacctt
     1861 cgaaaacgaa ccgccgcaga tccgcgccct gctcaagcaa gcgttaacct atgaacgcag
     1921 cgccaacgag tacgacggcg aatggcaggc ggccaacctc tattgccggg cttcgcgcct
     1981 gggcagtccg gaagccgagt atcggctcgg tatgttgtac gccttcggca agggtgtgcc
     2041 caagaatcgc ggtctcgccg cttcgctgtt ctcgctggcc tcctcacaag ggcattttga
     2101 ggcgcaaaag atgctggaga ccatcaacta caccaccagc gatctgccag cttgcgtgtt
     2161 gcaggccgtc gcgccggaga aagcgctggc cgcgatcgat gtgcagatcg acaacctgcc
     2221 cgagaacaag cgctgggtcg tcgatctggt cagcaccatg tccgactggt acgacatcga
     2281 tcccaaactc atcctgtcca tcatccaggt cgaatcaaac ttcaaggtcg gcgcgcagtc
     2341 caacaaggca gcacaggggc tgatgcaact gatccccgcg acggcggagc gcttcaacgt
     2401 caagaacgca tacgacgcgg cgcagaacat caagggcggc atcgcttata tccgctggct
     2461 gtt
//
LOCUS       DATMTW010000064         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_72619, whole genome shotgun sequence.
ACCESSION   DATMTW010000064 DATMTW010000000
VERSION     DATMTW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72619"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /gene="hemW"
                     /locus_tag="VN084_01290"
     CDS             <1..1044
                     /gene="hemW"
                     /locus_tag="VN084_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWT28117.1"
                     /translation="EIPERAYVDALIADLEQSVPNIWDRKVRIVFFGGGTPSLFSPDA
                     IDRILSQVRALVPLEYEAEITLEANPGTVDESHFRGFREAGVNRLSIGIQSFDPRYLT
                     ALGRIHDERQACSAAEHALATFDQVNLDVMYALPQQSLEDALADAETACALGPQHLSF
                     YHLTLEPNTPFHRSPPSLPDDDTSAEMQERIEQLLASHGYQHYETSAFAKSGMACRHN
                     LNYWQFGDYLGIGAGAHSKLSYHDRITRERRHKHPNHYLEGAGKGQAVAEVWTIPTDE
                     LGFEFMMNGLRLTGGFDIGLFQQRTGRAIAEIEPALNTAKARGLIVIEGDAIKPTLLG
                     QRFLNELLQLFLQ"
     gene            complement(1063..2556)
                     /locus_tag="VN084_01295"
     CDS             complement(1063..2556)
                     /locus_tag="VN084_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28118.1"
                     /translation="MLAATGWLATQGWAEPDLDTTADTAETMVWPMLPGESVNQLAAL
                     FYPGDKAMQRRFADRTRALSAAMAPTLDPATPFTQAAVIVIPDIKALSRQAKGFKRAS
                     TPRPDKLRMSYQIAEAGSVMVTPQMEAEYESLSQRNRALKDNLQKLNARLAELQLKMA
                     AMLDQRASGTASTAGQDMPTPERQPAAQSSLPAPATPPVSTPASTPVTLAPSALPDPA
                     STSAAAVASPAKAVTAIKPLSAPATPPQPTISQPATSQPSTSLPQDSAPLSASYWGDD
                     DLKGLLMQFAMLVLGLILALFAWRWLRKQLAKKITEATDEQLDGLRKNTFSADESGFF
                     NTQQPFHVADDMGVLSVEEFDSVVDEARILVSMDRINEAISLLRQHLDEQPRSSLQPW
                     VYLLDLYRTLDKQEAFTELAKRFHLAFNVMAPQWEQKQIAMVVATTLEEFPHIIDQLQ
                     SMWPNPESRDYLEALIKDNREGERAGFSIEVAKEIALLISILETRDS"
     gene            complement(2663..>3466)
                     /gene="argH"
                     /locus_tag="VN084_01300"
     CDS             complement(2663..>3466)
                     /gene="argH"
                     /locus_tag="VN084_01300"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWT28119.1"
                     /translation="LPLGAAALAGTSYPIDRQMVAKALGFDGVCENSLDAVSDRDFAI
                     EFTAAAALVMTHLSRLSEELILWSSPRFGFIDIADRFCTGSSIMPQKKNPDVPELVRG
                     KTGRVTGHLMALLTLMKSQPLAYNKDNQEDKEPLFDTADTLLVTLEIYADMLTGIKVD
                     KAAMRAAATEGFATATDLADYLVKKGMPFRDAHEVVALAVRHAADKGCDLSDLALAEL
                     QKFSSQIAADVFDVLTLEGSLASRDHIGGTAPTQVRQAIGRARKILGAH"
BASE COUNT          657 a         1062 c         1059 g          688 t
ORIGIN      
        1 gagatccctg aacgtgccta tgtcgatgcg ctgatcgccg accttgaaca atccgtccca
       61 aatatctggg accgcaaagt acgcattgtc ttcttcggcg gcggcacccc aagcctgttc
      121 tcgcctgatg ccatcgaccg catccttagc caggttcggg cgctggtgcc gctggaatat
      181 gaggcagaga tcacgctcga agccaatccg ggtacggtcg acgaatcaca tttcaggggc
      241 tttcgtgagg ctggcgtcaa tcgcctgtcg atcggcatcc agagtttcga tccgcgctat
      301 cttaccgccc ttggccgcat ccacgacgaa cggcaggcct gcagcgcagc agaacatgcg
      361 ctggcgacct tcgatcaggt gaatctcgac gtgatgtacg ccttaccgca acaatcgcta
      421 gaggacgcgc tggccgacgc tgagacagcc tgcgcactcg gtccgcaaca cttgtcgttc
      481 tatcacctaa cgctggagcc caatacgccg ttccaccgaa gcccacccag cctgcccgac
      541 gacgacacca gtgccgagat gcaggagcgc atcgaacagt tgctcgcgtc gcatggttac
      601 cagcattacg aaacctcggc cttcgccaag tcgggcatgg catgccgaca taatctcaac
      661 tactggcaat tcggtgatta cctcggcatc ggcgcgggcg cgcacagcaa gctcagctac
      721 catgaccgca tcacgcgcga gcgccggcac aagcatccca atcattacct ggaaggcgct
      781 ggcaagggcc aggcggtggc ggaagtatgg accatcccga ccgatgagct gggttttgaa
      841 ttcatgatga acgggctgcg actgactggc ggtttcgata tcggcttgtt ccagcaacgc
      901 accggcagag ccatcgccga gatcgagccg gcgttgaata ccgccaaggc acgcggcctg
      961 atcgtcatcg agggcgacgc gatcaaaccc accctgctcg gccaacgttt cctcaacgag
     1021 ttattgcagt tattccttca gtaacctgca gggtagcaag cgctaactat cgcgggtttc
     1081 caggatggag atcagcaacg cgatctcctt cgccacttcg atgctgaaac cggcgcgctc
     1141 gccttcccgg ttgtccttga tcagcgcttc caggtaatcc ctggattccg ggttaggcca
     1201 catcgattgc aactggtcga tgatgtgcgg gaactcttcg agcgtggtcg ccaccaccat
     1261 cgcgatctgc ttttgctccc attgcggcgc catcacattg aacgcgagat ggaagcgctt
     1321 cgccagctcg gtgaatgcct cctgtttatc cagcgtacgg tagaggtcca gcaggtagac
     1381 ccagggttgc agcgaggatc tgggttgttc gtcgagatgc tggcgcagca ggctgatggc
     1441 ttcattgatg cgatccatac tgaccaggat gcgcgcctca tcgaccaccg aatcgaactc
     1501 ctcgacagac agcacgccca tgtcgtctgc cacatggaat ggctgctggg tattgaagaa
     1561 accgctctcg tcggcgctga acgtgttctt gcgcaagccg tccagttgct cgtcggtcgc
     1621 ctcggtgatc tttttcgcca gctgcttgcg tagccagcgc caggcgaaca gcgccaggat
     1681 cagaccgagt accagcatgg cgaactgcat cagcaagcct ttaaggtcat catcgcccca
     1741 gtagctggca gatagggggg ctgaatcctg tggcaatgaa gtcgatggct gcgaggtcgc
     1801 tggctgtgag attgttggct ggggcggtgt agcgggcgcg gataacggct tgatggccgt
     1861 gacagccttt gcgggtgacg cgacagccgc cgctgatgtg cttgccggat ccggcagggc
     1921 ggacggcgcc agcgtgactg gggtcgaggc tggagtcgag actggcggcg tagcaggtgc
     1981 cggaagcgag gactgcgctg caggctgacg ctcgggcgtg ggcatatcct ggccggccgt
     2041 gcttgctgtg ccgcttgcgc gctggtccag catcgccgcc atcttcaact gtagttctgc
     2101 caacctggcg ttgagtttct gcaggttatc cttgagcgcg cgattgcgct ggctcaggct
     2161 ttcatactcc gcttccatct gcggtgtgac catcacgcta cctgcctccg cgatctgata
     2221 gctcatgcgc agcttatccg ggcgcggcgt cgacgcacgc ttgaagcctt tggcctggcg
     2281 tgacagggcc ttgatatccg ggataacgat cacggcagct tgagtgaatg gcgtcgccgg
     2341 gtcgagcgtc ggcgccatgg ccgcgctgag ggcgcgggtc cggtcggcga aacgtcgctg
     2401 catcgccttg tcgccgggat agaacaaagc cgccagctgg ttcacgctct cgcccggcag
     2461 catgggccag accatggtct cggccgtgtc ggctgtcgtg tcgagatcgg gctccgccca
     2521 tccttgcgta gccagccagc cagtcgcagc gagcatggcc agccacgaca gatagacaga
     2581 aaaaacccgg ccgcggccgg atcttcggag ggatgtgata aagcggccaa cgctgcctga
     2641 gccggaacgt tggcctgcca agttaatgcg ctcccagtat ttttcgcgcc cgtccgatcg
     2701 cctgcctgac ttgggtcggc gcagtgccgc cgatatggtc acggctcgcc agtgagcctt
     2761 cgagcgtcag cacgtcgaac acgtcggccg cgatctggct actgaacttc tgcaattcgg
     2821 caagcgcaag gtcggacaag tcacaaccct tgtcggcggc gtgacgcacc gcaagcgcga
     2881 ccacctcatg ggcgtcacgg aacggcatcc ctttcttcac caggtaatcc gcgagatcag
     2941 tggccgtggc gaagccttcg gtcgcggctg cacgcatggc cgccttgtcg accttgatgc
     3001 cggtcagcat gtcagcgtag atctccagcg tcaccagcaa ggtgtcggcg gtatcgaaca
     3061 agggctcctt gtcttcctgg ttatccttgt tataggccaa cggttgcgat ttcatgagcg
     3121 tcagcaaggc catcagatgg ccagtgacac gccccgtctt gccgcgcacc agttccggca
     3181 cgtccgggtt cttcttctgc ggcatgatgg aggaaccggt gcagaagcgg tcggcgatgt
     3241 cgataaagcc gaaacggggg ctcgaccaga ggatcagctc ttccgacagt cgtgacagat
     3301 gggtcatgac cagtgccgcg gcggccgtga attcgatggc gaaatcacgg tcggagaccg
     3361 cgtccaggga attctcgcag acgccatcga accccagtgc cttggcgacc atttgccgat
     3421 cgatcgggta gctggtgccg gccagcgcgg ctgcgcccaa aggcaa
//
LOCUS       DATMTW010000065         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_206999, whole genome shotgun sequence.
ACCESSION   DATMTW010000065 DATMTW010000000
VERSION     DATMTW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206999"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..277
                     /gene="pqqA"
                     /locus_tag="VN084_01305"
     CDS             206..277
                     /gene="pqqA"
                     /locus_tag="VN084_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HWT28120.1"
                     /translation="MWTKPAATEMRFGFEVTMYVCNR"
     gene            complement(390..1823)
                     /locus_tag="VN084_01310"
     CDS             complement(390..1823)
                     /locus_tag="VN084_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWT28121.1"
                     /translation="MLPSYVLLDLETTGATPLRDRITEIALVRFDHGIETARWQTLVN
                     PETPIPPFIQNLTGITDEMVRDAPTFAEVAPLLLDYFEGSVLAAHNARFDHGFLKSEY
                     KRIGISLRQKVLCTVKLSRKLYPEHYRHGLDAIIQRHGLTCSARHRAMGDVEMLIGFL
                     QAASADLGIEQVQATAESLMRGPSLPPGLDHTLLDDLPESPGVYLFYGESPGDSQRQE
                     GQAHAGYELPLYIGKSVNIRSRVLSHFSSDHSSTKEMRITQEIKRVEWRETAGEFGAL
                     LLESRLIKEMQPIYNRRLRNERQLCSWRIADTADTRPLVTLVRESEIEATMVGQLFGT
                     FRSKRQAVEVLQQIAENHGLCEKLLGLENGKGPCFAHQLRRCKGVCAGKEAPEIHYLR
                     LQQALAAHRLKTWPYEGRIALREYDSASNQTQIHVFEHWCHLVTASDEATLHESLETR
                     RPLAFDLDTYKLLLKELHRNRLEIIRL"
     gene            1917..2669
                     /locus_tag="VN084_01315"
     CDS             1917..2669
                     /locus_tag="VN084_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT28122.1"
                     /translation="MNRTYPTMAALTLLLCLSNANAATLEDARQAIEAHDFKTASTIY
                     HELADQGDAKAQYNLGLMYSRGDGVKQNYNEALKWYRLSAEQGFAEAQYNAGIILLRP
                     EWVKPDYAESIKWYTKAAEQGHLRSQVALGVAYLRGEVVRYDPEAAQKWFTTAAEQGD
                     KEAQFDLSSMYLELEGPDRNMVLGYMWLLISSDAAHGEAKRNYKAEKRIRFVESKMTP
                     EQIAEGKKLAQECVAMQLKGCAANPFKAVAAS"
BASE COUNT          591 a          828 c          768 g          577 t
ORIGIN      
        1 gtttcggctt tgaagtaacg ctgtatgtat gtaaccgtta aggctacatc gccaaatcgc
       61 gcaaccgccg aggttgttat gattcaggca tcatgacgta tcgttcttca aatcaatcta
      121 accagttatc caaccagatc gggtggtcgc tccagcactt ttggaactgc acctgcccga
      181 tctgcgctaa ataaggagag tcatcatgtg gaccaaacca gccgctactg aaatgcgttt
      241 tggctttgaa gtcacgatgt acgtgtgcaa tcgctaacac gacatttgtg taaagtaagt
      301 caagcgaacg acttcctctg aagtcgcatc gtagaataac gggagcctct ggctcccgtt
      361 ctcatttcag ggggtcacgt tcaatgcggc tagagccgaa tgatctccag cctgttccgg
      421 tgtagttcct tcagcagcaa tttataggta tcaaggtcaa acgccagcgg acgcctggtc
      481 tcgagcgatt cgtgcagggt cgcttcatcg ctggccgtca ccagatggca ccagtgttcg
      541 aacacgtgga tctgcgtctg gttggaggcc gaatcatatt cgcggagggc gatcctgcct
      601 tcatacggcc aggttttcag ccggtgcgcc gccagtgcct gctgcaatct caggtagtgg
      661 atctctggcg cctccttgcc tgcacaaacg cccttgcagc gccgcaactg atgggcgaag
      721 cacgggccct tgccgttctc cagccccagt agcttctcgc acaggccatg gttttcggcg
      781 atctgctgca gaacctccac cgcctggcgt ttgctgcgga aggtgccgaa caattgcccg
      841 accatcgtcg cctcgatctc gctttcgcgt accagcgtga ccaggggccg cgtgtcggca
      901 gtatcggcga tacgccacga acacagttgc cgctcgttgc gtaaccgacg gttgtagatc
      961 ggctgcatct ccttgatcag ccttgattcg agcagcaagg caccgaactc gcctgcagtc
     1021 tcgcgccatt ccacgcgctt gatctcctgg gtgatgcgca tctccttggt tgagctgtgg
     1081 tcgctgctga aatgcgacag cacgcggcta cggatattga cgctcttgcc gatataaagc
     1141 ggcagctcgt agcctgcatg ggcttgcccc tcctggcgct ggctatcacc cggactttcg
     1201 ccatagaaca ggtaaacccc ggggctttcc ggcaggtcgt ccagcaatgt gtgatccagg
     1261 cccggcggca agctggggcc acgcatcagt gactcggccg tggcctggac ctgttcgatc
     1321 cccaagtctg cgctcgccgc ctgcagaaaa ccgatcagca tctcgacatc gcccatggcg
     1381 cggtgcctgg ctgagcatgt cagaccgtga cgctggatga tggcgtcgag tccgtgacgg
     1441 taatgctccg gatagagctt gcgcgaaagc ttcacggtgc agagcacctt ctgccgcagg
     1501 ctgatgccga tgcgcttgta ctcgctcttg agaaagccgt gatcgaaccg cgcgttatgc
     1561 gccgccagta cgctgccctc aaagtaatcg agcaacagcg gcgcgacttc ggcaaacgtc
     1621 ggcgcatcgc gcaccatttc gtcggtgatg ccggtcagat tctggatgaa cggcgggatc
     1681 ggcgtttcgg gattcaccag cgtctgccaa cgcgccgtct cgataccgtg gtcgaagcgc
     1741 accagcgcga tctcggtgat acggtcgcgc agcggtgtcg ccccggtggt ttcgaggtcg
     1801 agcaatacat aagatggaag catgggcacg agtttgaact attacagcca gaataatcaa
     1861 actgactagc aattcaccag aataattccg gcgccgccga ccataaggag aacgacttga
     1921 accgcaccta ccccacgatg gcagcgctga ccttgctgct ttgcctatcc aatgccaatg
     1981 ccgccacgct cgaagatgcc cggcaggcca tcgaagcaca cgatttcaaa actgcctcca
     2041 ccatctacca cgagcttgcc gatcagggtg acgccaaggc gcaatacaat ctcggcctca
     2101 tgtattcccg tggcgatggc gtcaagcaga actacaacga ggcgctcaaa tggtatcggc
     2161 tctctgccga acaaggattt gccgaagccc aatacaacgc cggcatcatc ctgttgcgac
     2221 ctgagtgggt gaaaccggat tacgcagaat ccatcaagtg gtacaccaag gcagccgagc
     2281 aaggccacct gcggtcgcaa gtcgcgctgg gcgtcgccta cctgcgtggc gaagtcgttc
     2341 gctacgaccc ggaagccgcg cagaaatggt tcaccacggc cgccgaacaa ggcgacaagg
     2401 aagcccagtt cgatctctcc agcatgtatc tcgagctcga aggcccagac cgtaacatgg
     2461 tgctgggtta tatgtggcta ctgatctcgt ccgatgccgc acacggcgag gccaaacgca
     2521 actacaaggc cgaaaagcgc atccgcttcg tcgaatcgaa gatgacgccg gaacagatcg
     2581 ccgaaggcaa gaagctcgct caggaatgcg tcgccatgca gctcaaaggc tgtgccgcca
     2641 accccttcaa ggctgtggct gccagctagt cggtgcgcgc gtattgcaga caataaaaag
     2701 gccgggtttc cccggccttt ttattgatgc tgatttcgac tgcttatact tcgtactgca
     2761 cgcg
//
LOCUS       DATMTW010000066         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_643136, whole genome shotgun sequence.
ACCESSION   DATMTW010000066 DATMTW010000000
VERSION     DATMTW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643136"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..539
                     /locus_tag="VN084_01320"
     CDS             27..539
                     /locus_tag="VN084_01320"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HWT28123.1"
                     /translation="MSHYNVKPIRLDFKLSLRLFVLLVCVALGSCAIVLYLPIRPLLD
                     QAFGQPFAQVLSQGIKLGAVILILLTLRQHGLKQWQRTPQACKALCLDSKGAWQLEYA
                     DGSVHAVTILPTSFVAPYLTILHCRLAGRWSSQSVVILPDAVDAEAYRRLRVWLRWGY
                     QDSSDEAAGA"
     gene            complement(506..1543)
                     /locus_tag="VN084_01325"
     CDS             complement(506..1543)
                     /locus_tag="VN084_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWT28124.1"
                     /translation="MNTVKHWLKKAFNYFRHRHDVALLTSAFILLVVALFNPTVPIKR
                     DIYSYMLVADISQSMNVVDTTLNGKPATRAEYMRYMMKRVVASMPCGTSVSIGLFAGV
                     SVAAMYRPLEVCQNYDAIQDTITHLDWRTAWSGNSRIRNSMPNLARTLRSFPQSAQVV
                     FFTDGEEAPKLHVFNTQDLTNFQGGTDWLFVGIGSDKGTPIPKLDSENQLIGYWSGES
                     FAMQPGIAQISEANIGVRDDNVASAESDRYLSKLDEQYLKDLAKEINGMYVNGDSLHD
                     ILGAMKKQRPARRDTAAFELRWLLAAVAGLLFITAYLPKHPVKASRNAIQAWFSRRRK
                     PQAPAASSLES"
BASE COUNT          293 a          439 c          528 g          354 t
ORIGIN      
        1 cgatatctca agccgggttt tagggtatgt cacattataa cgtgaagccg atacggctgg
       61 atttcaagct ttcgctgcgg ctgttcgtgc tattggtgtg cgtcgcgctg ggtagctgcg
      121 ccatcgtgct gtacctgcct atccgaccat tgctcgatca ggcgttcggc cagccgtttg
      181 cacaggtgct cagccagggg atcaagctcg gagcggtgat cctgatactg ctgaccttgc
      241 gtcagcatgg cctgaaacag tggcagcgta cgccgcaggc ctgcaaggcg ctatgtctgg
      301 atagcaaggg ggcttggcag cttgaatatg ccgacggcag cgtgcatgcg gtgacgatcc
      361 tgccgacgag cttcgtcgcg ccttatctca ccatcctgca ttgtcgcctt gcgggacgct
      421 ggtcttccca gtcggtggtc atcctgccgg atgcggtcga tgcggaagcc tatcgccgct
      481 tgcgggtttg gctgcgctgg ggatatcagg actcgagtga cgaagcggcg ggcgcctgag
      541 gtttccggcg gcgtgagaac caggcctgga tcgcgttacg gctggccttg accggatgct
      601 tgggcaggta ggccgtgatg aacagcaggc cagccacagc agcgagtagc cagcgtaatt
      661 caaaagcagc ggtatcgcgg cgggccggac gctgtttctt catggcgccc aggatgtcgt
      721 gcaggctgtc gccattcaca tacatgccgt tgatctcttt cgcgaggtcc ttcaggtatt
      781 gttcatccag cttggaaagg tagcgatcgc tctcggccga ggcgacgtta tcgtcgcgca
      841 caccgatgtt cgcttccgag atctgggcga tccccggctg catggcgaag ctctcaccag
      901 accagtagcc gatcagctgg ttctcggaat ccagtttcgg gatcggcgta cccttgtcgc
      961 taccgatacc gacgaacagc cagtcggtgc cgccctggaa attggtcagg tcttgcgtgt
     1021 tgaacacgtg cagcttaggg gcttcttcgc catcggtgaa aaacaccact tgtgccgatt
     1081 gcgggaagga gcgcagcgta cgcgcgaggt tgggcatgct gttgcggatg cggctattgc
     1141 cggaccaggc agtgcgccag tcgagatggg tgatggtgtc ctggatggca tcatagttct
     1201 ggcagacttc gagcggccta tacatggcgg cgacactgac gccggcgaac aggccgatgc
     1261 tgacgctggt gccacagggc atggatgcga ccacgcgttt catcatgtag cgcatgtatt
     1321 cggcgcgcgt ggccggtttg ccgttgagtg tggtgtcgac cacgttcatg ctctgcgaga
     1381 tgtcggcgac cagcatgtag ctgtagatgt cgcgtttgat cggcacagtc gggttgaaca
     1441 aggccaccac cagcagtatg aacgcagacg tcagcaaggc gacgtcatgc cgatggcgga
     1501 aataattgaa ggccttcttg agccagtgtt taacggtgtt catgggcgca actcacaagt
     1561 gctgggcaat ggtaggcgat aggctttggc caggccgcct caagccgggc cggc
//
LOCUS       DATMTW010000067         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_273625, whole genome shotgun sequence.
ACCESSION   DATMTW010000067 DATMTW010000000
VERSION     DATMTW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273625"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..171
                     /locus_tag="VN084_01330"
     CDS             10..171
                     /locus_tag="VN084_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28125.1"
                     /translation="MEGSLSGKLVDDNQRPLGDSQIEKIRQQLKVIHATMVARGIMPG
                     SPGALRLFS"
     gene            193..2250
                     /gene="ligA"
                     /locus_tag="VN084_01335"
     CDS             193..2250
                     /gene="ligA"
                     /locus_tag="VN084_01335"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWT28126.1"
                     /translation="MAEFDPLAAQGSLFSTSPGQPEAEIARLIAQIREWDHQYYVLDA
                     PTVPDSEYDRVFRQLQALEHANPHLITPDSPTQRVGGLPHKTFSAITHRVPMLSLNNA
                     LDENEASAFDRRVRESLGIDQVEYAVEPKFDGLAITLTYEHGRFVQGATRGDGFTGED
                     VTANLRTIRALPMRLNTPNPPALFEVRGEVLMLKQDFERLNQAQAARGDKLFANPRNA
                     AAGSLRQLDPNVTANRPLTFFAYGLGAVEGLSLPATHSGVMDLLASLHLPVARERATV
                     SGLQGLLDYYEAIGAKRQALPYDIDGVVYKVNASRQQAELGFVSRAPRWAVAHKFPAQ
                     EALTEVLDITVQVGRTGAITPVARLKPVFVGGVTVTNATLHNEDEVRRKDIHIGDTVS
                     VRRAGDVIPEIVNVMIERRPDDAKRFVMPTTCPICGSHIVKPQDEAVARCSGGLFCPA
                     QRKQAILHFASRRAMDVEGLGDKLVDQLVDQGLVHKLSDIYQLDLATLSGLDRMAERS
                     AQNILDALESSKATTLARFIYALGIRNVGEATAKDLARHFGNLQALQAADVTTLEQVP
                     DVGPVVAESLFDFFSEAHNREVISQLQAAGIHWEDTPGKVGVDGVLSGKTLVLTGTLP
                     NMSRDAAKELIEAAGGKVSGSVSKKTDYVVAGAEAGSKLEKAQGLGVAILDEAGLLAL
                     LQS"
     gene            2262..3077
                     /gene="cysQ"
                     /locus_tag="VN084_01340"
     CDS             2262..3077
                     /gene="cysQ"
                     /locus_tag="VN084_01340"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWT28127.1"
                     /translation="MDQVIDKKWLPQIMQIAREAGDAIMQVYRGEIDVQRKDDNSPLT
                     MADLAAHTLIEARLKQMTPELPILSEESASIPYDERRRWTRYWLVDPLDGTREFIKRN
                     DEFTVNIALIDEGRVVMGVVYAPALDMLYFAAQGFGAFRQARAEAATRISARPLDTTS
                     LIIAGSRSHAGERMQGFLQRVGEKLSTPKSISLGSSLKICLVAEGSADLYPRLGLTSE
                     WDTAAAQCVLEQAGGHLLEWNGSPLRYNGKDSLLNPEFLACGATLHDWKQYLE"
     gene            3082..>3678
                     /locus_tag="VN084_01345"
     CDS             3082..>3678
                     /locus_tag="VN084_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28128.1"
                     /translation="MDFRSAAFFALTLFAGIPAQAADTLAAKVDQCDRAVSAGDTAKA
                     LALSQEVLGQEANNREALLCKGRAYGASGNYQEALLALQAAEQLSKTPLDHIVARTLM
                     GNVQKSAGDFDAAMQTYQQSLALAKREANGQLQRINLNLIGEIQLATQHLDQALENFL
                     AGSKLAANDNERADNYARIADVYNKQGKNDQAIEYQVKT"
BASE COUNT          797 a         1124 c         1094 g          663 t
ORIGIN      
        1 gcaaaacaga tggaaggcag cctcagcggc aaactggtgg acgataacca gcgcccgctc
       61 ggtgacagcc agatcgaaaa gatccgccag cagctcaaag tgatccatgc caccatggtc
      121 gcccgcggca tcatgccggg cagcccgggc gccctgagac tgttctcttg agtcgttttg
      181 cagtgaagca ccatggctga attcgatcct ttggccgcgc aaggcagcct gttctccaca
      241 tcgccaggac aaccagaggc tgagatcgcc cgcctgatcg cgcagatccg ggaatgggat
      301 catcaatact acgtgctcga cgccccaacc gtacctgata gcgaatacga ccgtgtcttt
      361 cgccagctgc aggccctaga acatgccaat ccgcatctga tcacaccaga ctcgcccacg
      421 cagcgtgtcg gtggcttgcc acacaagact ttcagtgcga tcacccatcg cgtgcccatg
      481 ctgtcgctca acaatgcgct cgacgagaac gaggcaagcg ccttcgaccg ccgcgtaagg
      541 gaaagcctgg gtatcgacca ggtggagtac gccgttgagc ccaagttcga tggcctcgcc
      601 atcacgctga cctatgaaca cggccgcttc gtacagggcg ccacccgcgg cgacggcttt
      661 acgggagagg atgtcacggc caatctgcgc accatccgcg ccttgccgat gcggctcaac
      721 acgcctaatc caccagcgct gttcgaagta cgaggcgaag tgctcatgct gaagcaggat
      781 tttgaacgcc tgaaccaggc ccaggcagcc cgtggcgaca agctcttcgc caatccgcgt
      841 aacgcggcgg cgggcagcct gcgtcagctt gaccccaacg tcacggccaa tcgcccactg
      901 acgttcttcg cttacggcct cggggcggtc gaggggctat cgctgcctgc gacccatagc
      961 ggcgtcatgg atctgctcgc gtccctgcat ctgccggtgg cacgtgagcg cgcgaccgtg
     1021 tcaggtcttc agggattgct cgattactac gaagccatcg gcgccaagcg ccaggcattg
     1081 ccttacgata tcgatggcgt cgtatacaag gtcaatgcct cacggcaaca ggctgaactg
     1141 ggcttcgttt cacgtgcgcc acgctgggct gtcgcccaca agttcccggc acaggaggcc
     1201 ttgacggagg tgctggatat cacagtgcaa gtaggccgta ccggggccat cacgccggtc
     1261 gcgcgcctca aaccggtatt cgtcggtggc gtcactgtca ccaatgccac gctgcacaac
     1321 gaggacgagg tccggcggaa agatatccat atcggggata ccgtcagcgt gcgcagggca
     1381 ggggacgtca tccccgagat cgtcaatgtc atgatcgaac gccggccgga cgatgcaaaa
     1441 cgattcgtca tgccgaccac gtgtccgatc tgcggatcac atatcgtcaa accacaggat
     1501 gaggctgtgg cacgctgtag cggcggtttg ttctgtccag cccaacgcaa gcaggcgatc
     1561 ctgcacttcg cttcacgtcg cgcgatggat gttgaaggcc tgggcgacaa gctggtcgac
     1621 cagttggtgg accagggcct ggtgcacaag ctatcggata tctatcaact cgacctggct
     1681 actttgtccg ggctcgatcg catggccgag agatcggcgc agaacattct cgacgcactg
     1741 gaaagcagca aggcgacgac attggcacga ttcatctacg ccctcggcat ccgcaatgtc
     1801 ggtgaagcca cggccaagga tctggcgcgg catttcggta atctgcaggc gctacaagcg
     1861 gcggatgtca cgacactgga acaggtgccg gatgtcggcc ccgtggtagc ggaatccctc
     1921 tttgatttct tcagtgaagc gcataaccgc gaagtgatct cgcaactgca ggctgccggc
     1981 atccattggg aggatacgcc aggaaaggta ggcgtcgatg gcgtgctcag cggcaagacg
     2041 ttggtactga caggtacatt gccgaacatg tcgcgcgatg cggcgaagga gctgatcgaa
     2101 gctgccggcg gcaaggtcag cggcagcgta tcgaagaaga ccgactatgt cgtcgccggc
     2161 gccgaggcgg gcagcaagct cgagaaggcc cagggacttg gtgtcgccat cctggatgag
     2221 gctggcttgc tggcgctgct ccaaagttag gatcgggcgc catggatcag gttatcgaca
     2281 agaaatggct gccgcagatc atgcaaatcg cccgcgaagc cggcgatgcg atcatgcagg
     2341 tctaccgtgg cgagatcgac gtacaacgta aggatgataa ttcgccactg acgatggccg
     2401 atcttgcggc acataccctc atcgaagccc gcctcaagca gatgacaccg gaattgccga
     2461 tcctttcgga agaatccgcc agcatcccct acgacgagcg caggcgctgg acgcgctact
     2521 ggctggtcga cccgctggat ggcacgcgcg agttcatcaa gcgcaatgac gaattcacgg
     2581 tcaatatcgc gctgatcgac gagggcaggg tggtcatggg ggtcgtctat gcgccggcgc
     2641 tggatatgct ctactttgcc gcccagggct tcggcgcctt tcggcaggca cgtgccgaag
     2701 ccgcgacacg tatctctgcg cgtccgctgg atacgacttc attgatcatc gccggtagcc
     2761 gttcccacgc gggcgaacgc atgcagggct tcctgcagcg cgtcggcgag aaactttcca
     2821 cgccaaaatc aatctctctc ggtagctcgt tgaaaatctg cctggtagca gaaggcagcg
     2881 ctgacttgta tccacggctg ggattgacct cggaatggga tacggctgca gcacagtgcg
     2941 tgctggagca ggccggtggc cacttgcttg aatggaatgg ctcgccgctg cgctacaacg
     3001 gcaaggattc cctgctcaac ccggaattcc tggcttgtgg cgccacgctg catgactgga
     3061 aacaatattt ggaatgacat gatggacttc agatccgccg ctttttttgc actgacgcta
     3121 ttcgccggca taccggcaca ggccgccgac acgcttgccg ccaaggtcga tcaatgtgat
     3181 cgcgcagtct ccgcggggga taccgcaaaa gccctggcct tgtcgcagga ggtgctgggt
     3241 caggaggcga acaaccggga agcactgcta tgcaagggtc gagcctacgg cgccagcggc
     3301 aactaccagg aagccttgct tgccctgcaa gccgcggagc agctatccaa aaccccactt
     3361 gatcacatcg tcgcgcgaac cctgatgggt aacgtgcaaa aaagcgctgg cgatttcgat
     3421 gctgccatgc agacctacca gcagagcctc gccttggcca agcgcgaggc gaacggccag
     3481 ctccaacgca tcaacctcaa ccttatcggc gagatccagc tcgcaacgca gcatctagac
     3541 caggcgctcg agaatttcct tgccggcagc aagctcgcgg ccaatgacaa cgagcgtgca
     3601 gacaactatg cgcgcatcgc cgatgtctac aacaagcaag gcaaaaacga ccaggccatc
     3661 gaataccagg tcaagacc
//
LOCUS       DATMTW010000068         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_39312, whole genome shotgun sequence.
ACCESSION   DATMTW010000068 DATMTW010000000
VERSION     DATMTW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39312"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..810
                     /locus_tag="VN084_01350"
     CDS             265..810
                     /locus_tag="VN084_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWT28129.1"
                     /translation="MLISCGSSGQKAPTLYGTDISGADFAQTLQLTDHNGKPRSLADF
                     KGKVVALFFGYTHCPDVCPTTMADLAAALKLMGDRAAEVQVLFVIVDPERDTQEVLAQ
                     FVPSFNPTFIGLYGTPAQTAETAKNFKIFYEKQVDPGKSSYTIDHSAGVYAFDKQGKI
                     RVYLKFGQKPEELAHDLSSLL"
     gene            914..2365
                     /gene="ccoN"
                     /locus_tag="VN084_01355"
     CDS             914..2365
                     /gene="ccoN"
                     /locus_tag="VN084_01355"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWT28130.1"
                     /translation="MATSSVAAAGSADQYNYDIVRRFTIMTLVWGAIGMFVGVYIASE
                     LAFPFLNFDNPYITFGRLRPVHTGAVIFGFGGSALFATSYYVVQRTCQARLFSDGMAN
                     FTFWGWQAVIVCMALGNMMGYSQGREYAEMEWPIDILIEIVWVTYLIVFVSTIMKRKQ
                     PHIYVANWFYLAFILATALLHTFNNLAVPIDLFSMKSYSLFSGAQDAMTQWWYGHNSV
                     GFFLTAAFLGMMYYFVPKQAGRPVYSYRLSVLHFWAIIFLYMWAGSHHLHWTAIPDWT
                     STLAATFSIMLLLPSWGGMINGILTLSGAWDKLRTDPIMRFLIVALSFYGMSTFEGPM
                     MSLKDVNALSHYTDWTIGHVHSGALGWVAMITFGSLYHLVPRLWNTQMHSMRLVNVHF
                     WLATVGVLLYITAMWISGIMQGLMWRAFDDFGNLQYSFVESVAAAHPFYIMRAVGGAF
                     FLTGMLIMCYNMYRTIRQGNAEEAEASISPAVA"
     gene            2390..>2586
                     /locus_tag="VN084_01360"
     CDS             2390..>2586
                     /locus_tag="VN084_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HWT28131.1"
                     /translation="MNFNHDKIERNLGILLILTMLAISAGGLVEIVPLFFIKETVEKV
                     DGVRPYSPLELRGRDIYVREG"
BASE COUNT          499 a          774 c          697 g          616 t
ORIGIN      
        1 taccagatta gaccatcgcg gtcagccatc gacatctttg cttctcctag cctgccgggt
       61 gcgaacccgc gcataaaaaa gcgtaaattg taaacgagac gccccctgct tgaacaggga
      121 tactccaatg tatttcaagg gcatgcatca aatcccgtgc acaataaatg gcgactgcat
      181 cgtgctagac taccggtcta gcgttatatg aagtccgcca tgtccagaat ccttcgcagt
      241 gtttgcatcg ccgccctggc gatcatgctg atttcctgcg gctcgtccgg ccagaaagca
      301 ccgaccctgt acggcaccga tatctctggc gccgatttcg cccagacgct ccagctcact
      361 gaccacaacg gcaagccccg cagcctggcg gatttcaagg gcaaggtggt ggcgttgttc
      421 ttcggttata cccattgtcc ggatgtctgc ccgaccacca tggctgatct tgctgccgcg
      481 ctcaagctca tgggcgaccg cgccgccgag gtgcaggtgc tgttcgtgat cgtcgatcct
      541 gaacgggata cgcaggaggt gctcgcgcag ttcgtgcctt cgttcaaccc gaccttcatc
      601 ggactctatg gcaccccggc acagaccgca gagacggcaa agaatttcaa gatcttttat
      661 gagaagcagg tggatccggg caagagcagc tataccatcg atcatagcgc cggggtttat
      721 gcgtttgata agcaaggaaa aatccgcgtc tatctcaagt tcgggcaaaa gcccgaagag
      781 ctggcgcacg atctttcctc cttgttgtga ggcatttcgc ctcgtgtttt aagttgtcgt
      841 gccgtgacta tagagaatat aatcacgggc ttttcgattt cagttttcgg cttttggatt
      901 aggagacgta gtaatggcaa catcaagcgt cgcagcagca ggtagcgcgg accaatataa
      961 ctacgatatt gttcgccggt tcaccatcat gaccttggtt tggggcgcga tcggtatgtt
     1021 cgtcggtgta tatatcgcct ccgaactcgc ttttccgttc ctgaatttcg ataaccccta
     1081 catcaccttc ggtcgactgc gtcctgtcca caccggcgcc gtgatcttcg gcttcggcgg
     1141 cagcgccttg ttcgccacct cctactatgt cgtgcagcgc acctgtcagg cgcgcctgtt
     1201 cagtgacggc atggccaatt tcacgttctg gggctggcag gcggtcatcg tctgcatggc
     1261 gctcggcaac atgatgggct acagccaggg ccgtgaatac gcggaaatgg aatggccgat
     1321 cgacatcctg atcgagatcg tctgggtgac ctacctgatc gtcttcgtct ccaccatcat
     1381 gaagcgcaag cagccgcata tctatgtggc taactggttc taccttgcct ttatcctcgc
     1441 caccgccttg ctgcatacct tcaataacct ggcggtgccg atcgacctct tctccatgaa
     1501 gtcctacagc ctgttctccg gcgcacagga cgccatgacc cagtggtggt acggccacaa
     1561 ctcggtgggc ttcttcctca ccgcggcctt cctcggcatg atgtattact tcgtgcccaa
     1621 gcaggctggc cgcccggttt attcctatcg cctgtcggtg ctgcacttct gggcgatcat
     1681 cttcctctat atgtgggctg gttcgcacca cttgcactgg accgcgatcc ctgactggac
     1741 ctcgaccctg gccgcgacct tctccatcat gctgctgctg ccttcctggg gcgggatgat
     1801 caacggtatc ctgaccttat caggcgcctg ggacaagctg cgcactgacc cgatcatgcg
     1861 tttcctgatc gtggcgctgt cgttctacgg catgtccacc ttcgaaggcc cgatgatgtc
     1921 gctgaaggac gtcaacgcac tgtcgcatta caccgactgg accatcggcc acgttcactc
     1981 cggcgcgctc ggctgggtgg cgatgatcac cttcggttcg ctctaccacc tggtgccgcg
     2041 cctctggaac acccagatgc acagcatgcg cctggtcaac gtgcacttct ggctggcgac
     2101 tgtgggcgta ttgctctaca tcaccgccat gtggatctcc ggcatcatgc agggcctgat
     2161 gtggcgtgcg ttcgatgact tcggcaacct gcagtattcc ttcgtcgagt ccgtcgctgc
     2221 cgcgcatccg ttctatatca tgcgtgcggt cggcggcgcg ttcttcctga ccggtatgct
     2281 gatcatgtgc tacaacatgt acaggacgat ccgtcagggc aacgctgaag aagcggaagc
     2341 cagcatcagc ccggcagtgg cttgagatat aaggttagtg gagtagaaca tgaattttaa
     2401 tcacgacaag atcgaacgta atctcggcat cctgctgatc ctgaccatgc tggccatcag
     2461 tgccggcggc ctggtggaga tcgtgccact gttcttcatc aaggaaaccg tcgagaaggt
     2521 cgatggcgtg cgcccctaca gcccacttga gctgcgcggc cgcgatatct acgtgcgcga
     2581 aggttg
//
LOCUS       DATMTW010000069         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_106594, whole genome shotgun sequence.
ACCESSION   DATMTW010000069 DATMTW010000000
VERSION     DATMTW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106594"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1181
                     /locus_tag="VN084_01365"
     CDS             216..1181
                     /locus_tag="VN084_01365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HWT28132.1"
                     /translation="MQKLIDTQSIVSQATVARHSQHTVVIFGEVLFDDFPDGAVLGGA
                     PFNVAWHLQAFGMHPVVISRTGNDAQRERIIDNMQRWGMDTIGMQSDPLHPTGRVAVH
                     EDDHGHRFEILAEQAYDYIHPTIARMVELSVHPRLLYYGTLAQRNKVSRRALAALLRS
                     ASVPRLLDLNLRAPWYDTRLINRSLESADIVKMSQEELAVLAKSLRLRGEDPVAHGDA
                     LVKRFALDRILVTCGDEGSWLLDKQGMLARESAEVGDIKVIDTVGAGDAFSAVYITGM
                     LLDWPIYITMQRASAFAAAVCGVRGATPQDPAFYDRFRNAWGVPA"
     gene            1178..>3017
                     /locus_tag="VN084_01370"
     CDS             1178..>3017
                     /locus_tag="VN084_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWT28133.1"
                     /translation="MSTPQNPKLHILMISVHGLIRGKEMELGRDADTGGQITYVVELA
                     KALGRHAAVGQIDLLTRLVDDPQIAPDYAVPEEPLADNVRILRLPCGPRRYLRKESLW
                     PHLDQMVDRCLHYLRQQGKLPDIIHTHYADAGYVGLQLSNLLGIPQIHTGHSLGRPKR
                     QRLLDSGRKELTIERQFNLGQRIRVEEEVLSHASLIVASTRQEVTDQYGMYQVPDQKY
                     SVIPPGTDTSLFFPPGRKPIQPEVTEMIDRFLSEPDKPAVLAICRPDTRKNIPGLLKA
                     YGEHPELQARANLILVAGTREDIRQMGEAQQKVLNDVLLDIDRYDLWGKVAIPKNCSV
                     EYIPELYRLVARRRGVFVNPALTEPFGLTLIEAAASGIPFVATEDGGPRDIVSNCHSG
                     HLVDPLDSASIAEAIFGILQDRKQWQKLSRNGIAGVRRHYTWDGHVAKYLKSVGQVLH
                     QSRKRMRREMALSLRLKRTPIPLAQKALISDIDNTLLGDEQGLRELITLLRKHSDQMA
                     FGIATGRSIDSAVKILKKWQVPIPDVLITSVGSEIHYWPSQRLDLGWSHHISYQWRRD
                     ALETFFSAVPGLTLQAAENQREFKLSYNVDPEQMPPIKELYKQLRQL"
BASE COUNT          611 a         1014 c          880 g          512 t
ORIGIN      
        1 agcgcttaac ccatttgtca tgaaaaaagt gtgaactcga ccgaatcgag gctgtcccgt
       61 tatatgggtg cacggctcgc acctgttgtt catcatcgct gcccacatca caaattaacg
      121 cgcagcctca ataatttaaa aaatgcatgg tttgtgccaa ggacaaaatc cggcacacaa
      181 gccactgctc tattagggaa tggactcgct tcgttttgca aaaactcatc gacacgcaat
      241 ctatcgtcag tcaggccacc gtcgcccgac atagccagca taccgtcgtc atcttcggcg
      301 aggtgctgtt cgacgacttt ccagacggcg ccgttctagg cggcgccccg ttcaacgtcg
      361 cctggcatct gcaggccttc ggcatgcatc ccgtcgtgat ctcgcgcacc ggcaacgacg
      421 cgcagcgcga acgcatcatc gacaacatgc agcgctgggg catggacacc atcggcatgc
      481 agtccgaccc cttgcacccg accggccgcg tcgccgtgca tgaggacgac catggccacc
      541 gttttgagat ccttgccgaa caggcctatg actatatcca cccgaccatc gcccgcatgg
      601 tcgagctcag cgtacatccg cgcctgctgt attacggcac cctcgcccag cgcaacaagg
      661 tctcgcgccg tgcgctggcg gcattgctgc gtagcgccag cgtaccgcgc ctgctggacc
      721 tcaacctgcg cgcaccctgg tatgacaccc gtctcatcaa tcgttcgctg gaaagcgccg
      781 atatcgtcaa gatgagccag gaggaacttg ccgtactggc caagagcctg cgcctgcgcg
      841 gcgaagaccc ggtggcccac ggcgatgcgc tggtcaagcg tttcgcgctg gaccgcatcc
      901 tcgtcacctg cggcgacgaa ggctcatggc tgctggacaa gcaaggcatg ctggcacgcg
      961 aatcggccga ggtcggcgac atcaaggtca tcgacaccgt cggggccgga gacgcgttct
     1021 cggcggtcta catcacgggc atgctgctcg actggccgat ctacatcacc atgcagcgcg
     1081 ccagcgcctt tgccgcagca gtctgtggcg tgcgtggcgc cacgccgcag gatcccgctt
     1141 tctacgaccg gttcaggaat gcatgggggg taccggcatg agcaccccac agaatccgaa
     1201 gctgcacatc ctcatgatca gcgtgcacgg cctgatccga ggcaaggaga tggagctggg
     1261 ccgcgacgcc gacaccgggg gccagatcac ctatgtcgtc gaactggcga aggcgctcgg
     1321 tcgccatgcg gccgtcggcc agatcgacct gctgacgcgc ctggtggacg acccgcaaat
     1381 cgcgccggac tatgccgtgc cggaagaacc gctggcggat aatgtgcgca tcctccgcct
     1441 gccgtgcggg ccgcgccgct acctgcgcaa ggagtcgctg tggccgcatc tggaccagat
     1501 ggtcgaccgc tgcctgcact acctgcgcca acagggcaaa ttgccggaca tcatccacac
     1561 ccactatgcc gacgccggct atgtcgggct gcagctctcc aacctgctgg gcatcccgca
     1621 gatccatacc ggccattcgc tgggccggcc gaagcgccaa cgcctgctcg acagcggccg
     1681 caaggagctg accatcgagc gccagttcaa cctggggcag cgtatccgcg tcgaggaaga
     1741 agtgctgtcg cacgcctcgc tcatcgtcgc cagcacacgc caggaagtca ccgaccagta
     1801 cggcatgtac caggtaccgg atcagaaata tagcgtgatc ccgccgggga ccgatacctc
     1861 gctgttcttc ccgccgggtc gcaagccgat ccagccggaa gtgacggaga tgatcgaccg
     1921 cttcctgagc gagcccgaca agccggcagt gctcgccatc tgccgaccgg acacgcgcaa
     1981 gaacatcccg gggctgctca aggcctatgg cgaacatccc gaactgcagg cacgcgccaa
     2041 cctcatcctc gtcgccggca cacgcgagga tatccgccag atgggcgagg cccagcaaaa
     2101 ggtgctcaac gacgtgttgc tggatatcga ccgttacgac ctctggggca aggtcgccat
     2161 ccccaagaat tgcagcgtgg aatacatccc cgagctttac cggctggtcg cgcgtcgtcg
     2221 tggcgtgttc gtcaatccgg ccctgaccga gcctttcggc ctgacgctga tcgaggccgc
     2281 cgccagcggc atcccgttcg tcgccaccga agacggcggg ccgcgcgata tcgtcagtaa
     2341 ctgccacagc gggcacctgg tcgacccgct cgacagcgcc agcatcgccg aggcgatctt
     2401 tggcatcctg caagaccgca agcaatggca gaagctctct cgcaacggca tcgccggcgt
     2461 ccggcggcat tacacctggg atggccatgt cgccaaatac ctgaaatccg tcggtcaggt
     2521 gttgcaccag tcgcgcaagc gcatgcggcg cgagatggcg ctgagcctgc gcctcaagcg
     2581 cacgccgatc ccgctcgcgc aaaaagcctt gatcagcgat atcgacaaca ccttgctggg
     2641 cgacgagcag ggcttgcgcg aactcatcac gctgttgcgc aagcacagcg accagatggc
     2701 gtttggtatc gccaccggcc gcagcatcga tagcgcggtg aagatcctga agaaatggca
     2761 ggtgccgatc cccgatgtgc tcatcacctc ggtcggcagc gagatccatt actggccttc
     2821 gcagcgactc gacctgggct ggagccacca catcagctac cagtggcgtc gcgacgcgct
     2881 ggaaactttc ttcagcgcgg taccgggcct gaccttgcag gccgccgaga accagcgtga
     2941 attcaaactc agttacaacg tcgaccccga gcagatgccg ccgatcaagg agctgtacaa
     3001 gcagctgcgt cagttaa
//
LOCUS       DATMTW010000070         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_609945, whole genome shotgun sequence.
ACCESSION   DATMTW010000070 DATMTW010000000
VERSION     DATMTW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609945"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="glyA"
                     /locus_tag="VN084_01375"
     CDS             <1..1122
                     /gene="glyA"
                     /locus_tag="VN084_01375"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWT28134.1"
                     /translation="VMQAQGSQLTNKYAEGYPGKRFYGGCEFVDQIEQIAIDRLKKLY
                     GAEYANVQPHSGSQANQAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGKLFNIVA
                     YGLNENEEIDYDEMERVAIENKPKLLIGGASAYALRFDWARMAEIAKKVGAYFMVDMA
                     HYSGLIAAGVYPNPVPHADFVTSTTHKTLRGPRGGIIMAKAEYEKMLNSSVFPSLQGG
                     PLMHVIAGKAVAFLEASQPEFKAYQEQVLKNAAAMAETLAARGLRIISGRTESHMFMV
                     DLRPKGLTGKAADAALGLAHITVNKNAIPNDPESPFVTSGIRIGSPAITTRGFKEEEA
                     RQVANLIADVLDDPANEAVIAATKQKVHALTSRFPVYGA"
     gene            1122..1439
                     /locus_tag="VN084_01380"
     CDS             1122..1439
                     /locus_tag="VN084_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWT28135.1"
                     /translation="MNTCPGCGVAHADMHPQMPLAQLPNLGTVSAEWLAQAGLHTLAD
                     LAGIGAIEAYRRVQALGFKPSLNLLYALEGALHDEHWLQTKRHRKLDLLARLQAAKEH
                     ATS"
     gene            1436..1936
                     /gene="nrdR"
                     /locus_tag="VN084_01385"
     CDS             1436..1936
                     /gene="nrdR"
                     /locus_tag="VN084_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWT28136.1"
                     /translation="MKCPFCGADDTQVIDSRVNDEGDSIRRRRRCAECDKRFTTYETA
                     ELHLPQVVKQNGNREEFNRDKLRLSFTRALHKRPVPTEYVDRAIEHIVQKILGKGERE
                     IMARDLGESVMQELRLMDKVAYIRFASVYRSFQDVDDFHDAIRDLDKHGREIPKRRST
                     DSAEES"
     gene            1954..2331
                     /locus_tag="VN084_01390"
     CDS             1954..2331
                     /locus_tag="VN084_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28137.1"
                     /translation="MKIIKILCVALSLITLLITIVFFCWWAMKDYFVFGDQRFDQVRW
                     ITAAPTADNRCYRGDMAHDLQSRLLLKGMPRQFALAMLGRPDWDDGSQIEYDLGHCLW
                     DTHGLRLYFGDDGNLIQSRIVQH"
     gene            2331..>2608
                     /locus_tag="VN084_01395"
     CDS             2331..>2608
                     /locus_tag="VN084_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWT28138.1"
                     /translation="MARVASDPKPNEAYMRRALALAERGLYTTTPNPRVGCVIVQGDR
                     IVGEGAHLRAGEHHAEVDALRQAGDLSRGADVYVTLEPCSHYGRTPPCA"
BASE COUNT          524 a          834 c          767 g          483 t
ORIGIN      
        1 gtgatgcagg cgcaaggttc gcagctgacc aacaagtacg ccgagggtta tccgggcaag
       61 cgcttctatg gtggttgtga attcgtcgac cagatcgagc aaatcgccat cgaccgcctg
      121 aagaaactgt acggtgcaga atacgctaac gtacaaccgc actccggttc gcaggcgaac
      181 caggcggtgt acttctccat cctcaagccg ggcgataccg tcatgggcat gaatctcggt
      241 cacggcggtc acctgaccca tggttctcct gccaacctgt ccggcaagct gttcaacatc
      301 gttgcctatg gtctcaatga gaacgaagag atcgactacg acgagatgga acgcgtcgcc
      361 atcgagaaca agccgaagct gctgatcggc ggtgcttccg cttatgccct gcgcttcgac
      421 tgggcacgca tggccgagat cgccaagaag gtcggcgcct atttcatggt cgatatggcc
      481 cactactccg gcctgatcgc tgcgggtgtc tatccgaacc cggtgccgca cgctgatttt
      541 gtcacctcga ctacgcacaa gacgttgcgt gggccgcgtg gcggcatcat catggccaag
      601 gccgagtacg agaagatgct gaattccagc gtgttcccct cgctgcaggg cggcccgctc
      661 atgcacgtga tcgccggcaa ggccgtggct ttccttgagg cctcgcagcc tgaattcaag
      721 gcgtaccagg aacaggtgct gaagaatgct gcagccatgg ccgagacctt ggctgcacgc
      781 ggcctgcgta tcatctccgg ccgtaccgaa tcgcacatgt tcatggtcga cctgcgtccc
      841 aaggggctca ccggtaaagc ggccgacgct gcattgggcc tggcgcatat caccgtcaac
      901 aagaacgcga tccccaacga cccggaaagc ccgttcgtca cctccggcat ccgcatcggt
      961 tcgccggcca tcacaacgcg cggcttcaag gaagaagaag cgcgccaggt ggccaacctg
     1021 atcgccgatg tgctggacga tcccgccaac gaggctgtca tcgcggccac caagcaaaag
     1081 gtgcacgcgc tgaccagccg gttcccggtc tacggcgcct gatgaacact tgcccgggtt
     1141 gtggcgtagc ccatgctgac atgcatccgc agatgccgtt ggcgcaactg ccgaacctgg
     1201 gtacagtaag tgccgaatgg ctggcccagg ccggcttgca tacactggcg gatctggccg
     1261 gcattggcgc catcgaggcc tatcgccggg tgcaagcgct agggttcaaa cccagcctca
     1321 acctgctgta cgcgctggaa ggcgccctgc acgacgagca ttggttgcag accaaacgcc
     1381 accgtaaact cgatctgctg gcccgcctgc aggcagccaa ggagcacgcg acgtcatgaa
     1441 atgcccgttc tgtggtgccg acgataccca ggtcatcgac tcacgcgtca acgacgaggg
     1501 agacagcatc cgccgtcgcc gtcgttgtgc cgagtgcgac aagcgtttca ctacctatga
     1561 gacggccgag ttgcacctgc cgcaagtggt caagcagaac ggtaatcgtg aggaattcaa
     1621 ccgcgacaag cttcgcctgt ccttcacccg tgccctgcac aaacgccccg taccgactga
     1681 atatgtggat cgcgccatcg agcacatcgt gcagaagatc ctcggcaagg gcgagcgcga
     1741 gatcatggcg cgcgaccttg gcgagagcgt catgcaggaa ctgcgcttga tggacaaggt
     1801 ggcctatatc cgtttcgcct cggtctatcg tagcttccag gatgtggacg atttccacga
     1861 cgcgatccgc gacctcgaca agcacggtcg cgagatcccg aagcgacgca gcaccgatag
     1921 cgccgaagag tcctgatcct ccaccggtgc cccatgaaaa tcatcaagat cctttgcgtg
     1981 gcgctttccc tgatcaccct gctgatcacc atcgtctttt tctgctggtg ggcgatgaag
     2041 gattatttcg ttttcggcga ccagcgcttc gaccaggtac gctggatcac agccgcacca
     2101 acggccgaca accgctgcta tcgcggtgac atggcgcatg acctgcaaag ccgcctgttg
     2161 ttgaaaggca tgccgcggca gttcgcgctg gccatgctgg gccgcccgga ctgggatgat
     2221 ggtagccaga tcgaatacga tctcggtcac tgcctgtggg atacccacgg cctgcgcctg
     2281 tatttcggcg atgacggtaa cctgatccag agtcggatcg tccagcattg atggctcgcg
     2341 tcgcttccga tcccaagccg aacgaagctt acatgcgccg cgcgctggcc ctggccgagc
     2401 gcggacttta taccaccacg ccgaatccgc gcgtcggctg cgtcatcgtc cagggtgatc
     2461 gcatcgtcgg cgagggcgcg cacctgcgag ccggcgagca tcacgcggaa gtcgacgcct
     2521 tgcgacaggc gggcgatctc tcccgtggcg ccgatgtcta tgtcaccctc gaaccctgca
     2581 gccattacgg ccgcacgccg ccctgcgc
//
LOCUS       DATMTW010000071         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_273803, whole genome shotgun sequence.
ACCESSION   DATMTW010000071 DATMTW010000000
VERSION     DATMTW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273803"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="tgt"
                     /locus_tag="VN084_01400"
     CDS             <1..612
                     /gene="tgt"
                     /locus_tag="VN084_01400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWT28139.1"
                     /translation="PARQLSDDAPSAERNALFGIIQGGMYEDLRDESLAGLVEIGFDG
                     YAIGGLSVGEPKEDMLRILAHTAPRMPADKPRYLMGVGTPEDLVAAVSRGVDMFDCVM
                     PTRNARNGWLFTRHGDIKLKNARYKLDTGPLDPDCQCYTCQNFSRAYLHHLHRLGEIL
                     GARLNTIHNLHYYQELMSDMRQAIEQDRFDAFVTRFAEQRGAK"
     gene            683..1000
                     /gene="yajC"
                     /locus_tag="VN084_01405"
     CDS             683..1000
                     /gene="yajC"
                     /locus_tag="VN084_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWT28140.1"
                     /translation="MFISNAYAAPAAASPDWTSFIPLVVIFVLFWFMLIRPQMKQAKE
                     QRKMIEALQKGDEVTTVGGVLGKVTKVGEAFVSLEIATGVEINVQKHTIQTLLPKGTI
                     KSL"
     gene            1037..2866
                     /gene="secD"
                     /locus_tag="VN084_01410"
     CDS             1037..2866
                     /gene="secD"
                     /locus_tag="VN084_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWT28141.1"
                     /translation="MNRYPLWKYMLVLITLVLGLLYSIPNLFGESPAVQVSSAKNTVK
                     VDTTVMGEVETALKEGSVKYETIFMDANGIKIRLKDPETQLKAKDVLQAKLGNDYTIA
                     LNLLSRSPDWLRSIGALPMYLGLDLRGGVHFLLQVDMKAALDQAAERYVGDFRISLRK
                     ERIAYLGISRDAQTVIVRFGNMEELQKAEKLLNKEFADLVFKESDSGSEKLLKASLSP
                     PAQTRIQEFALKQNIQTLHNRINELGVAEPIIQQQGLDRVVVQLPGVQDTAKAKEILG
                     RTATLEIRLVDEEKSDLVMLERAAKGQVPFGDEYFEDRDGRPLLVKKSVVLTGDYITD
                     AGPGVDQQNGGSVVNVTLDGRGARIFKQVTRENVKKRMAILLIEKGKAEVITAPVINE
                     EIGGGRVQISGMASPQEATDVSLLLRAGALAAPMEIIEERTVGPSMGEENIKRGVHST
                     LWGFAAIAVFMIIYYMVFGTISVLALGINLLLLVALLSMLQATLTLPGLAAIALTLGM
                     AIDANVLINERIREEVRNGNTPQAAIHAGYDRAFDTILDSNVTTLIAGLALFMFGTGP
                     VKGFAVVHVLGILTSMFSAVVVSRAVVNLVYGYRRKINKLTIG"
     gene            2883..3401
                     /locus_tag="VN084_01415"
                     /pseudo
     CDS             2883..3401
                     /locus_tag="VN084_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830792.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
BASE COUNT          713 a         1065 c          994 g          629 t
ORIGIN      
        1 ccggcacgac aactatcaga cgatgcgcca tcagcagagc gcaatgcgct gttcggcatc
       61 atccagggcg gcatgtacga ggacctgcgc gatgaatcac tggccggtct ggtggagatc
      121 ggcttcgacg gctacgccat aggcggcctc tcggtcggcg aacccaagga agacatgttg
      181 cgcatcctcg cccacaccgc gcccaggatg cctgccgaca agccacgtta cctgatgggc
      241 gtcggcacgc ctgaggacct ggtcgcggcg gtctcgcgtg gcgtggacat gtttgactgc
      301 gtgatgccga cgcgcaacgc gcgcaacggc tggctgttca cgcgtcatgg cgacatcaag
      361 ctcaagaacg ccagatacaa gctggatacg gggccactcg accctgattg ccagtgctat
      421 acctgccaga acttcagccg cgcctatctc catcacctgc accggctggg ggagatcctc
      481 ggcgcgcgcc tcaacaccat ccacaacctg cactattacc aggaactgat gtccgatatg
      541 cgccaggcca tcgagcagga ccgcttcgac gctttcgtca cccgcttcgc cgagcagcgc
      601 ggggcgaagt gatgcgcgcg cgggctggca ctatgttaga attttgcgct tcaagtgatt
      661 cacaggatta aggaaggtta ccgtgtttat cagcaacgca tacgcagcac ccgcagccgc
      721 atcccccgac tggaccagct tcattccgct ggtcgtgatc ttcgtcctgt tctggttcat
      781 gctgatccgt ccacagatga agcaggccaa ggaacagcgc aagatgatcg aagcgctgca
      841 gaaaggcgat gaagtcacca ccgtcggcgg cgtgctgggc aaggtcacca aggtcggcga
      901 agcgttcgtc agcctggaga tcgccaccgg cgtcgagatc aacgtccaga agcacaccat
      961 ccagaccctg ctgcccaaag gcaccatcaa gtccctgtaa ccctgccgtc aacggcatag
     1021 cctcgcgagt ctcgccatga atcgttaccc cttatggaag tacatgctgg tgctcatcac
     1081 cctggtgctg ggcctgctct attccatacc caacctgttc ggtgaatccc cggccgtcca
     1141 ggtctccagc gccaagaata ccgtcaaggt cgataccacc gtcatgggcg aggtggaaac
     1201 cgcgctcaag gaaggctcgg tcaagtacga gaccatcttc atggacgcca acggcatcaa
     1261 gatccgcctc aaggatccgg agacccagct caaggccaag gacgtactgc aggcgaaact
     1321 gggtaacgat tacaccatcg cgctcaacct gctgtcacgc tcgcccgact ggttgcgcag
     1381 catcggcgcc ctgcccatgt acctgggtct ggacttgcgt ggtggcgtgc acttcctgct
     1441 gcaggtggat atgaaagcgg cactggacca ggccgccgaa cgttatgtcg gtgacttccg
     1501 catcagcctg cgcaaggaac gcatcgccta tctcggtatc agccgtgacg cacagaccgt
     1561 gatcgtgcgc ttcggcaaca tggaagaact gcagaaggcg gagaagctgc tgaacaagga
     1621 attcgccgac ctcgtcttca aggaaagcga cagcggcagc gagaagctgc tcaaggccag
     1681 cctcagcccg ccagcacaga cccgcatcca ggaattcgcg ctgaagcaga acatccagac
     1741 cctgcataac cggatcaatg agctcggcgt ggccgaaccc atcatccagc agcaaggcct
     1801 cgaccgtgtc gtggtgcaac tgcccggcgt gcaggatacc gccaaggcga aggagatcct
     1861 gggccgtacc gcgaccctcg agatccgcct ggtggatgaa gagaagtctg acctcgtcat
     1921 gctcgaacgg gcggccaagg gccaggtgcc gttcggcgac gaatacttcg aggatcgcga
     1981 tggccgtccg ctgttggtca agaagagcgt cgtcctcacc ggcgattaca tcaccgacgc
     2041 cggccccggt gttgatcagc agaacggcgg ttcggtcgtc aatgtgacgc tcgatggccg
     2101 tggcgcccgc atcttcaaac aggtcacacg cgagaacgtc aagaaacgca tggccatcct
     2161 actgatcgag aagggcaagg cggaagtcat caccgcgcct gtgatcaacg aagagatcgg
     2221 cggcggacgc gtgcagatct ccggcatggc cagcccgcag gaagcgaccg atgtctccct
     2281 gctgctgcgc gcaggcgcgc tggcagcccc gatggagatc atcgaggaac gtaccgtcgg
     2341 cccgagcatg ggtgaagaga acatcaagcg cggcgtgcat tcgacgctgt ggggctttgc
     2401 cgcgatcgcc gtgttcatga tcatctacta catggtgttc ggcaccatct ccgtgctggc
     2461 gctgggcatc aacctgctgt tgctggtggc cttgctgtcc atgctgcagg cgacactgac
     2521 gctgccgggc ctcgccgcga tcgcgctgac gctgggcatg gcgatcgacg ccaacgtcct
     2581 catcaacgag cgcatccgcg aagaagtgcg taacggcaat acgccgcagg cggccatcca
     2641 cgccggctac gatcgtgcct tcgacaccat tctcgactcc aacgtcacga cactgatcgc
     2701 cggtctcgcg ctgttcatgt tcggcaccgg tccagtgaaa ggcttcgctg tcgtgcacgt
     2761 gctcggcatc ctgacttcga tgtttagcgc cgtggtggtc tcacgcgccg tggtcaacct
     2821 cgtttacggc taccgccgca agatcaacaa actgaccatc ggttaatcaa gagcacaaga
     2881 tcatggaaat cttcaagatt aaaaaagaca tcccgttcat gagttatggc cgtctgacga
     2941 cgtccatctc attgatcacc ttcttgctgg cggtgttctt cctcgcgcat agaggcctta
     3001 acctcggcgt cgacttcacc ggcggcaccg tcatggaggt cagctatccg caggctgcga
     3061 acctggagca ggtgcgttcc gcggtcgaca gcgtcggcct caaggaggcg acagtacaga
     3121 acttcggcac cagcaaagac gtcctgatcc gcctgccggt gaaagagggc ctgaccagcg
     3181 cccagctctc ggaaaaggtg ctcaatgcgc tacgcaacgt cgatggttcg gtcgagatgc
     3241 gccgcgtcga gttcgttggc ccgcaggtag gataggaact ttacgacaac ggcgccctgg
     3301 cgatgctact ggtctgtatc ggtatcatgg gctatctcgc catgcgtttc gaatggcgtt
     3361 tcgctgtcgc tgccgtcatc gccaatatgc atgacgtcgt c
//
LOCUS       DATMTW010000072         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_173878, whole genome shotgun sequence.
ACCESSION   DATMTW010000072 DATMTW010000000
VERSION     DATMTW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173878"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1153)
                     /gene="lipA"
                     /locus_tag="VN084_01420"
     CDS             complement(188..1153)
                     /gene="lipA"
                     /locus_tag="VN084_01420"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWT28142.1"
                     /translation="MSESTTLKPAAKLPGVKESSAEKVARIPIKIVPQPAMRKPEWIR
                     MKVPDSARFQEIKQVLRDNKLHTVCEEASCPNIGECFSGGTATFMILGDICTRRCPFC
                     DVSHGKPLAPDANEPENLGSTIAQMRLKYVVITSVDRDDLRDGGAQHFVDCIASVRKH
                     SPAIKVEILVPDFRGRLDRAMAILTQAPPDVMNHNLETVPRLYKEARPGSDYHNSLNL
                     LKVFGEQHPQVPTKSGLMLGLGETDEEILDVMRDLRAHGVTMLTLGQYLQPSQHHLPV
                     KRFVTPERFTQFEREAMAMGFTHAACGPMVRSSYHADQQAHSAGV"
     gene            complement(1183..>1722)
                     /gene="lipB"
                     /locus_tag="VN084_01425"
     CDS             complement(1183..>1722)
                     /gene="lipB"
                     /locus_tag="VN084_01425"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWT28143.1"
                     /translation="ERDTATPDELWVNEHPPVYTLGLNRKGVRLPSSADIPVIAVDRG
                     GKITYHGPGQVVIYLLLDLQRRGLSVRQLVNVMEGAIVSLLAEHGIESAARPDAPGVY
                     VRDSKIASLGLRLKQQRCYHGLALNVDMDLSPFLAIDPCGYQGMQVTQLRDLGITLDA
                     AHASERLLRLLQDGLRQAS"
BASE COUNT          330 a          488 c          548 g          356 t
ORIGIN      
        1 aacagccaat actttattca tgacattcct gaaaagtgag ttagtaggaa tccgttctaa
       61 cgcatcaacc ggcatatggt tgaatggttt ttcacttttt ttaacatatt tttacaatta
      121 gcccgccgcc agcgagaaga cgccaagagt tggctctggg caggaccgac ccagcaggaa
      181 gcagaggcta gacgccggcc gagtgtgcct gctgatcggc gtggtacgag gaacgcacca
      241 tcgggccgca ggccgcatgg gtgaagccca tcgccatggc ctcacgctcg aattgcgtga
      301 agcgttccgg cgtgacgaag cgcttcaccg gcaaatgatg ctggctgggt tgcagatact
      361 ggcccagcgt gagcatggtg acgccgtggg cgcgcagatc gcgcatgacg tcgaggatct
      421 cttcatcggt ctcgcccagc ccgagcatga ggccggattt ggtcggcacc tgcgggtgct
      481 gttcgccgaa caccttgagc aggttgagcg agttatggta atccgaaccc gggcgcgctt
      541 ccttgtacag ccgcggtacg gtctccaggt tgtggttcat gacatcgggc ggggcctgcg
      601 tcaggatcgc catcgcgcgg tcaaggcgac cacggaaatc cggtaccagg atctcgacct
      661 tgatcgctgg cgagtgcttg cgcaccgagg cgatgcaatc gacgaagtgc tgggcgccgc
      721 cgtcacgcag gtcgtcacgg tccaccgagg tgatgaccac atacttgagg cgcatctggg
      781 cgatggtgct gccgaggttt tcgggttcat tggcatccgg ggccagtggc ttgccgtgcg
      841 acacatcaca gaaggggcag cgccgcgtgc agatatcacc gaggatcatg aaggtggctg
      901 tgccgccgct gaagcactca ccgatattgg ggcaggaggc ttcttcacac acggtatgca
      961 acttgttatc gcgcaatact tgcttgatct cttgaaaacg cgccgaatcg ggcactttca
     1021 tgcggatcca ttccggcttg cgcatggcgg gctgcggcac gatcttgatc ggtatgcgcg
     1081 cgactttttc cgcgctggat tccttgacgc cggggagctt cgctgcaggt tttagggtgg
     1141 tggattcgct catgagcgtg attatctcaa attctcaagc gcttagcttg cctgcctgag
     1201 gccgtcttgc agcaggcgca gcaatcgctc actggcgtgc gctgcatcca gcgtgatgcc
     1261 caagtccctg agctgcgtca cttgcatgcc ctgatagccg cagggatcga tggcgagaaa
     1321 gggcgagaga tccatgtcga cgttcaaggc caggccatgg taacagcgtt gctgcttgag
     1381 ccttaaacca agcgaggcga tcttgctgtc acggacgtag acacccgggg catcgggccg
     1441 ggcggccgat tcgatgccgt gctcggccag caatgaaacg atcgcgcctt ccatcacatt
     1501 caccagctgg cgcacgctga ggccgcggcg ctggaggtcg agtagcagat aaatgaccac
     1561 ctggcccggg ccgtgatagg tgatcttgcc gccgcggtcg accgcgatca ccgggatatc
     1621 ggcgctagaa ggcagccgca cgcccttgcg gttgaggccc aaggtataga ccggcgggtg
     1681 ttcgttgacc cagagttcgt cgggcgtggc cgtatcccgt tc
//
LOCUS       DATMTW010000073         6720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_643581, whole genome shotgun sequence.
ACCESSION   DATMTW010000073 DATMTW010000000
VERSION     DATMTW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643581"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VN084_01430"
     CDS             complement(<1..439)
                     /locus_tag="VN084_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWT28144.1"
                     /translation="MTMQRPSVSSFHPPKRILLGPGPSDVHQRVLDAMARPTVGHLDP
                     RFSEMLEDLKSLLRYTFQTENALTFALSAPGMIGMETCFVNLVEPGDKVIVCNNGVFG
                     RRMIENVRRCRGELVTVEDDWGRPVDPQKLEDALKANPDTRVVA"
     gene            complement(436..1473)
                     /gene="pyrC"
                     /locus_tag="VN084_01435"
     CDS             complement(436..1473)
                     /gene="pyrC"
                     /locus_tag="VN084_01435"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWT28145.1"
                     /translation="MTDTLTITRPDDWHLHLRDGDALRSVLPDTARQFARAIVMPNLR
                     PPVTTTEQAAAYRQRILDALPAGAVFEPLMTLYLTDNTPAEEIVKAKASGFVHGVKLY
                     PAGATTNSDSGVTSLDKCAGALAAMEKHGMPLLVHAEVTDADIDVFDREKVFIDRHMR
                     PLVARYPGLKIVFEHITTRDAAEFVAAASGQVGATITAHHLLMNRNAMFAGGMRPHHY
                     CLPVLKREEHRLALVRAATSGSAKFFLGTDSAPHPKTAKESACGCAGMYTAHAAIELY
                     AEVFAAAGALDKLEAFASFYGADFYGLPRNQGQVTLTRDAWTVPDELTLGAEALVPFR
                     AGQTIAWKLRG"
     gene            complement(1566..2414)
                     /gene="rsmI"
                     /locus_tag="VN084_01440"
     CDS             complement(1566..2414)
                     /gene="rsmI"
                     /locus_tag="VN084_01440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWT28146.1"
                     /translation="MSHSGTLYVVATPIGNLQDITLRALEVLKAVDVIAAEDTRHTAG
                     LLSHFAIQKKLVAVHEHNEHKAAEALLQRLQAGESIALVTDAGTPGISDPGAVVVDHL
                     RDAGIVVVPIPGASAVIAALSAAGIAVNGFTFHGFLPASGAQRRKVLESFKAMPQTLV
                     FYEAPHRVLESVADMAKVLGGERRLTIARELTKTFETFHRCPLSEAVAWLESDPNQQR
                     GEFVLLVEPAPAVEVAGIGEDAERVLKSLLAELPLKQAVKLATEITGLKKNALYERAL
                     HLTERT"
     gene            2451..4097
                     /locus_tag="VN084_01445"
     CDS             2451..4097
                     /locus_tag="VN084_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWT28147.1"
                     /translation="MTGLHAAEATNTKAPPGKKSPEASFMEGQACLQRTDLDCARLAL
                     NRIPAPSAYAKLLQGNIAAAQGDFDQTFRLLLPLQADQTLSKPATASLHASLGLAYEN
                     QPDTLRALEQYVETADYLGDAAARAENDQRIWHLLSQPSRSELIDIRGESYDSTIQGW
                     IDLALAAQSLDSDPTAIRGWRAAYPDHVARAFAKRQFGEGSTESASSIGTGAKPASLT
                     QPPSAPPTKLQGPVAVLLPFGVGAFYPVSDAIERGFVTAQGLHQDSNDIRLYPTDGSK
                     EAIEAVYAQAIGEGAAYVLGPLTRDEATALASQDRPVPTLELNQPETAATGRNVFSFG
                     LSIESEVSQIVRLARNAGMQTATIVSSGSPVAGRAANAFRDAWLADGGEILLQTGPDQ
                     TTTLGDMHERIKAQPTDMIFFAASPEEARAIRPFLDSGTPTFGISHLYSGLPFDPSDH
                     VMNAVRFVELPWVLDSDNADFKAYRDAAAELPPGEMQRWFALGADAYALLLQLSVSPL
                     QATTIDGLTGKLRISATGKISRELALGRFSADGVVLEKAP"
     gene            4094..4429
                     /locus_tag="VN084_01450"
     CDS             4094..4429
                     /locus_tag="VN084_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWT28148.1"
                     /translation="MNPAGQMAEALAAEYLQQKGLKLLSRNYRCRFGEIDLIMQDGPT
                     LVFVEVRMRSNRHFGSAAESITQTKQRKLMITAQHYLQQHGEKNSRFDAVIMSSLTAQ
                     DIQWLRDAF"
     gene            4608..5192
                     /locus_tag="VN084_01455"
     CDS             4608..5192
                     /locus_tag="VN084_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWT28149.1"
                     /translation="MHINFKMALTQSALVLIMATQLTACLPAVVGGAAAGGMMAADRR
                     TSGIYIEDEGIEIKAEKNIADQLGDKIHVNVTSYNRNVLITGEAEDEATKAKAEAIVK
                     AVDNVRSVTNELAVDMKSTIANRNNDAYVTSKVKARMLKEGRFPANYVKVVTESSVVY
                     LLGLVTHKEAEDAVEIASSTDGVYRVVKVFEYID"
     gene            5244..5555
                     /locus_tag="VN084_01460"
     CDS             5244..5555
                     /locus_tag="VN084_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HWT28150.1"
                     /translation="MSNDTPPAVNEIIIEGLTRAGKPFRPSDWVDRMCSTYASFGSDR
                     KLRYSPYLKPRVVNGVRCLAVDLKLKDVNPEGFSQLMHFAEENQLNVLDAAGNSIPVP
                     A"
     gene            complement(5585..5974)
                     /locus_tag="VN084_01465"
     CDS             complement(5585..5974)
                     /locus_tag="VN084_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWT28151.1"
                     /translation="MLEDTTGKRLALSDYKGKWVVVNYWATWCPPCLEEIPDLVNLYD
                     QRKDRDLVVIGVAFEYKNRQEVADYVDDMLMSYPIVLGNPQVAQQIGSAEVLPTTYIY
                     DPQGTLVKTHRGLITRRYIEQLIAGRR"
     gene            6178..>6720
                     /locus_tag="VN084_01470"
     CDS             6178..>6720
                     /locus_tag="VN084_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28152.1"
                     /translation="MPLSLKIPALSDKPLLIAETRASKLFEFIEKLPLSNPLQAASTL
                     LDEMEILNRQKVAPDGRIRALEIYRPVLLRIAELLATHYAAATLPLPAQAKEYAAAAE
                     SLWLELGYGYKLALVDQQGKLFSLVSSKGNALILQRAIESLGKLAMVYYQTYVTPPGN
                     LWQDLHQLYAHAAQESLIDIE"
BASE COUNT         1416 a         2130 c         1912 g         1262 t
ORIGIN      
        1 aggccacgac ccgggtgtcg ggattggcct tcaaggcatc ttccagcttt tgcggatcca
       61 ccggcctgcc ccagtcatcc tcgactgtca ccagttcgcc gcgacagcgt cgtacgttct
      121 caatcatgcg acggccgaac acaccgttat tgcagacgat aaccttgtcg cccggctcca
      181 ccagattcac gaagcaggtc tccatgccaa tcatgccggg cgccgatagc gcgaacgtca
      241 gcgcattctc tgtctggaag gtgtagcgca acaaggactt gaggtcctcc agcatctcgc
      301 tgaaacgtgg gtcgaggtgg ccgacggtcg gcctcgccat ggcatcaagt acgcgctgat
      361 gcacatcgga aggtcccggc ccgagcagga tgcgcttggg cgggtggaaa gagctgacgc
      421 tgggtctttg catggtcatc cccgcaactt ccaggcgatc gtctggcccg cccggaacgg
      481 caccagagcc tcggcgccca gcgtcagttc gtcggggacc gtccaggcgt ccctggtcag
      541 cgtgacctgc ccctgattac gcggcaggcc gtagaaatcc gccccataaa aactggcgaa
      601 ggcttcgagt ttgtcgagcg cgccagccgc cgcgaacacc tcggcataga gctcaatggc
      661 cgcatgggcc gtatacatcc cggcgcaacc gcaagccgac tccttggccg tcttgggatg
      721 cggcgcgcta tcggtcccca ggaagaattt ggcactgccc gaggtcgccg cacgcaccag
      781 cgccaggcga tgctcctcac gcttcagtac cggcaggcag tagtgatgcg gccgcatgcc
      841 tccggcgaac atggcattgc ggttcatgag caggtgatgg gccgtgatgg tggcgccgac
      901 ctggccggac gcagcagcca cgaactccgc cgcgtcgcgc gtggtgatat gctcgaagac
      961 gatcttcagg cccgggtagc gcgccaccag cggccgcatg tggcggtcga tgaacacttt
     1021 ttcgcgatcg aacacgtcga tgtcggcatc ggtcacttcg gcgtgcacca atagaggcat
     1081 gccatgcttt tccatggctg ccagggcgcc agcgcacttg tcgagactgg tgacgccgga
     1141 atccgaattg gtcgtggcgc ccgcaggata gagcttgacg ccgtgtacga atccgctggc
     1201 cttagccttg acgatctcct ccgctggcgt gttgtccgtg agatagagcg tcatcaatgg
     1261 ctcaaacaca gcgccggctg gcaaggcgtc gaggatgcgc tggcgatagg cggccgcctg
     1321 ttcggtagtg gtgaccggcg ggcgcagatt gggcatgacg atggcacggg cgaattggcg
     1381 cgccgtatcg ggcagtacgg agcgcagggc gtcaccgtca cgcaggtgca aatgccagtc
     1441 gtcagggcgg gtgatggtca atgtgtctgt catatacgaa tcttcaatac gcgaccgatg
     1501 cacgcccatg gatacagatc aaaaaaaccg tcgtatctgc acacaccttc gtccatccgt
     1561 cgctgttaag tcctttccgt cagatgcagc gctcgctcgt acagggcgtt cttcttcagc
     1621 cccgtgatct cggtcgccaa ttttaccgcc tgcttgagcg gcagttcagc caataaggat
     1681 tttaatacgc gttcggcgtc ttcgccgatc ccggcgactt cgaccgctgg cgcaggctcc
     1741 accagcaaga cgaattcgcc gcgctgctga ttgggatcgc tttcgagcca ggccacagcc
     1801 tccgacagcg ggcagcggtg gaaggtctcg aaggtcttgg tcagctcccg cgcgatcgtc
     1861 agccggcgct cgccacccag caccttggcc atatccgcga cactctccag cacacgatgc
     1921 ggggcttcat agaacacgag cgtctgcggc atggctttga atgattccag caccttgcgt
     1981 cgttgtgcgc cactggccgg caggaagcca tgaaaggtga atccgttcac ggcgataccg
     2041 gccgcggaca aggccgcgat gacggcgctc gctccaggga taggcacgac gacaatgccc
     2101 gcgtcgcgca gatgatcgac cacgacggct cccggatcgc tgatgcctgg tgtgccggcg
     2161 tcggtgacca gggcgatcga ctcgcctgct tgcagccgct gcaacaaggc ctccgccgct
     2221 ttgtgctcgt tatgttcatg caccgcgacc agtttcttct ggatggcgaa atgcgataac
     2281 agaccggccg tgtggcgcgt gtcttcggca gcgatgacgt ccaccgcctt cagcacctcc
     2341 agcgcgcgca gcgtgatgtc ctgcaggttt ccaatcggcg tggctaccac atataatgtg
     2401 ccgctatgac tcaacgtcta cttgcctttc tattgctgca attcgtcttc ttgaccggcc
     2461 tccatgcagc agaggccacc aacaccaaag ccccgcccgg caaaaaatcg cccgaggctt
     2521 ccttcatgga ggggcaggcc tgcctgcagc gcaccgacct cgattgtgca aggctggccc
     2581 tgaaccgcat ccccgcgcca tcggcctatg ccaaactact gcaaggcaat atcgcggcgg
     2641 cacaaggcga ctttgaccag acattccgac tgttgctgcc cctgcaggcc gaccagaccc
     2701 tgagcaagcc ggccaccgca agtttacacg caagcctagg cctagcctac gagaaccagc
     2761 cggataccct acgggcgctt gagcagtatg tcgagactgc cgactacctc ggcgatgccg
     2821 ccgcacgtgc ggagaatgac cagcgcatct ggcacctgct gagccaaccc agccgcagcg
     2881 aattgatcga tatccgtggc gaaagttacg acagcaccat tcagggctgg atcgacctgg
     2941 cattggcagc gcaatcgctg gacagcgacc caacggcgat ccgcggctgg cgcgcggcct
     3001 atccggacca cgtcgcccgc gccttcgcca aacgtcagtt cggcgaaggc tccacagaat
     3061 cggccagcag catcggcacg ggggccaagc cagcatcgct gacacagccg ccatctgccc
     3121 cgccaaccaa gctgcaaggg ccggtcgccg tgttgctgcc atttggcgtc ggagcgttct
     3181 atccggtctc ggatgccatc gaacgtggtt tcgtcactgc acagggcctg catcaggaca
     3241 gtaacgacat ccggctgtat ccgaccgacg gcagcaaaga ggccatcgaa gccgtctatg
     3301 cccaggctat cggcgaaggc gccgcttacg tactggggcc gctgacacgc gatgaagcca
     3361 cggccctcgc cagccaggac agacccgtgc ccacgctgga actcaaccag cccgagaccg
     3421 cggccaccgg caggaacgtc ttcagctttg gactctccat cgagtccgaa gtcagccaga
     3481 tcgtgcgtct tgcgcgcaat gcggggatgc aaacagccac catcgtttcc agcggcagtc
     3541 cggtcgctgg tcgcgcggcc aatgctttcc gtgatgcctg gctcgccgat ggcggcgaaa
     3601 tcctgttgca gaccggtcca gaccagacaa cgactctggg cgatatgcat gagcgcatca
     3661 aggcgcaacc tacagacatg atcttcttcg ccgccagccc ggaggaagcc cgagccattc
     3721 gacccttcct cgattccggt acaccgacct tcggcatctc gcacctctac tccggcttgc
     3781 cgttcgatcc gtccgatcat gtcatgaatg cggtgcgctt cgtcgagttg ccgtgggtgc
     3841 tcgatagtga caacgcagac ttcaaggcgt atcgcgatgc ggcggccgaa ttgccgccgg
     3901 gcgagatgca acgctggttc gccctggggg cggacgccta tgccctcttg ctacaactca
     3961 gcgtttcgcc gctgcaggcc accacgatcg acggcctcac tggcaagctg cggatctccg
     4021 ccaccggcaa gatcagtcgc gagctagcct tggggcggtt ctccgcagac ggcgtcgttc
     4081 tggaaaaagc gccttgaacc ctgcgggtca gatggcggaa gcactggccg ccgaatatct
     4141 gcaacaaaag ggcttgaaac tactatcacg caactatcgc tgccgctttg gtgagatcga
     4201 cctgatcatg caggacggtc caaccctggt gttcgtcgag gtgcgcatgc gcagcaaccg
     4261 tcattttggc agcgccgcag agagcatcac ccagaccaag caaaggaaac tcatgatcac
     4321 ggcccaacat tacctgcaac agcatggtga gaagaacagc cgtttcgatg ccgtgatcat
     4381 gagcagcctg accgcccagg atatccagtg gctcagggat gctttttgat ccgggccttg
     4441 tcgagcaaac acccatggaa gaactgaact tctgccacgg caagttatcc atagaatact
     4501 catccatcgg gcagggtcat ccacatggac tatcgattct gatccatcga tgcgttattc
     4561 tgcgcagcaa cccgaatacc ctcaatcgat aacgcaggag catacagatg catatcaatt
     4621 tcaaaatggc cctgacccag tcggccctgg tcctcatcat ggcgacccaa ctcaccgcct
     4681 gcctgccggc ggttgtcggc ggcgccgctg ctggcggcat gatggctgcg gaccgtcgca
     4741 cttcagggat ctacatcgaa gatgaaggca tcgagatcaa ggcggagaag aatatcgcgg
     4801 atcagctcgg cgacaagatc catgtcaacg tcaccagcta taaccgtaat gtgctgatca
     4861 ccggcgaagc cgaggacgaa gcgaccaaag ccaaagccga agccatcgtc aaggccgtgg
     4921 ataacgtgcg cagcgtcacc aatgaattgg cggtcgacat gaagagcacc atcgccaacc
     4981 gcaacaacga cgcctacgtc acctccaagg tcaaggcccg gatgctgaag gaaggacgct
     5041 tcccggccaa ttacgtcaag gttgtcaccg agtcctcggt ggtctacctg ctcggcctcg
     5101 tcacccataa ggaagcagaa gacgccgtgg agatcgccag cagtaccgat ggtgtctatc
     5161 gtgtggtaaa ggtgttcgaa tatatcgact gacgccctgc tataatttta tccaacctat
     5221 cggacacccc agaagagcag atcatgagca acgacacacc tcccgcggtc aatgagatca
     5281 tcatcgaagg cctgacccgg gccggcaaac ctttccgccc cagcgactgg gtggaccgca
     5341 tgtgcagcac ctatgccagc ttcggcagtg acaggaagtt acgctattcg ccctacctca
     5401 agccacgcgt ggtgaatggc gtgcgctgcc tggccgtcga cctcaaactg aaagatgtga
     5461 atcccgaagg gttcagccaa ctcatgcatt tcgccgagga gaaccagctc aacgtgctgg
     5521 acgctgccgg caacagcatc ccggtccctg cctgagctcg cggtcggcta gagtttgcga
     5581 cccactagcg ccggcctgcg attagctgtt cgatgtagcg ccgcgtgatg aggccgcgat
     5641 gggtcttcac cagcgttccc tgcggatcgt agatataagt cgtcggcaac acttcggctg
     5701 agccgatctg ctgggccacc tgcggattgc ccagcacgat cgggtaagac atcaacatat
     5761 cgtcgacgta atcggcgact tcctgacggt tcttgtattc aaaggcgacg ccgatcacga
     5821 ccaggtcacg atccttgcgc tggtcataga gattgaccag gtccgggatc tcttcgaggc
     5881 agggtgggca ccaggtcgcc cagtaattga ccacgaccca cttccccttg taatcactga
     5941 gcgcgagtcg ctttcctgtt gtatcctcta gcacaaagtt tgcttgagca aacacgttcg
     6001 tcgcaaaaca caacgcgaac actgcccata aacaacgacc aataaacggg gaagtcatga
     6061 caacatttcg aagattcggc atcaggctct gattctcaac tggattcagc atgttaaccc
     6121 gtgacaaagg tcacgggaaa ccctaacatc agccaatatt gtgggagtta tccttagatg
     6181 ccgctcagcc tcaagattcc agcgctttcc gacaagcctc tgctgatcgc ggagacccgg
     6241 gcctcaaaac tattcgagtt catcgaaaaa ctgccgctat ccaatccctt gcaagccgcg
     6301 tcgaccttgc tcgacgagat ggagatattg aaccggcaaa aggtcgcgcc ggacggccgt
     6361 atacgtgcgc tggagatcta tcgtcctgtg ctgctccgca tcgcagaact gctggccacc
     6421 cattacgctg cagccacctt gccgttaccg gcacaagcca aggaatatgc cgccgccgca
     6481 gagtcgctct ggctggaact gggctatggc tacaaactgg ccttggtcga ccagcaaggc
     6541 aaattgttca gcctcgtcag cagcaagggc aacgccttga tcctgcagcg cgccatcgaa
     6601 tcgctgggca aactggccat ggtctactac cagacttacg tcacgccgcc cggtaacctg
     6661 tggcaagacc tgcaccagct ttacgcgcac gccgcgcagg agtcattgat cgatatcgag
//
LOCUS       DATMTW010000074         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_274005, whole genome shotgun sequence.
ACCESSION   DATMTW010000074 DATMTW010000000
VERSION     DATMTW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274005"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /gene="arsA"
                     /locus_tag="VN084_01475"
     CDS             <1..1868
                     /gene="arsA"
                     /locus_tag="VN084_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HWT28153.1"
                     /translation="EEIERLGVTATPAFLIDGSLVHSGGMPTHDQVENWLRGKTPSFL
                     ERPTRHLFFTGKGGVGKTSLSTATALALADAGKRVLLVSTDAASNLDEMLGIELRNIP
                     TPVPGAPGMSVLNIDPDTAAESYRRRVLAQMDASASVTDRATVREQLSGACTTEIASF
                     DEFTSLLSDGAGDYDNIVFDTAPTGHTLRLLSLPQAWSGFLSGNDRGASCLGPHSGLK
                     MQEVRFKAAMDTLTDPGQTTVILVARADGSAIAEAARTSEELRTLGLNNQRLVINGVF
                     HASDPDDVVAAAITTLEQQALDEMPTCLQLLPQDRVPLRSFDTVGLSALRALLTVDAS
                     PIVSGNVGTEPIEPLPGLDALADELALAEHGLIMVMGKGGVGKTTIAAALALGLIQRG
                     KTVHLSTTDPAAHLVGTVDADVPGLQVDRIDPKLETQRYIDKVMASKSPGLNPQEQAL
                     LLEDCRSPCTEEVAVFHAFSKIVNEARSAFVVLDTAPTGHSLLLMDATGAYHRQTLRE
                     FGGRGGRLTTPLMRLQDETYTRIILVTLPEVTPVSQAAALQYDLRRAGVEPYAWVINK
                     SVLAAGTHDPLLAARMAGERKQMQRMAGGLAARLFVVPWRATPPIGNLELLKLVN"
     gene            complement(1961..2365)
                     /locus_tag="VN084_01480"
     CDS             complement(1961..2365)
                     /locus_tag="VN084_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28154.1"
                     /translation="MLIERIPAVQVLLGLVATAGLVACASPDGDHGHGRHRTAQPADT
                     AAGGSASTGGTMGSGMTGSNSACPMTNCDSAGASATGQRMMDKDAMCAMYRGMRDAPT
                     AQARQAMMARNMQDMSPEMRQRHMEMMQQQCQ"
     gene            2660..>3007
                     /locus_tag="VN084_01485"
     CDS             2660..>3007
                     /locus_tag="VN084_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWT28155.1"
                     /translation="MMDWTDRHCRVFHRQITKHTWLYTEMVTTGALVYGDVERHLRYD
                     DIEHPIALQLGGSDPADLAKSAKLGEQWGYDEINLNCGCPSERVQKGAFGACLMQEPQ
                     LVADCVKAMRDAVS"
BASE COUNT          594 a          878 c          922 g          613 t
ORIGIN      
        1 gggaagaaat cgagcgcctt ggcgttaccg cgacgcctgc cttcctgatt gatggttcgc
       61 tcgtgcacag cggcggcatg ccaacgcacg atcaagtgga aaactggctc aggggcaaaa
      121 cgccgagttt cctggagcgg ccgacccgtc acctattttt tacaggtaaa ggaggcgtcg
      181 gaaagacttc attatcgaca gccacggcac tggcgcttgc cgatgccgga aaaagggtac
      241 tgctggttag tacggacgcc gcatcgaacc tcgatgagat gttaggcatc gagcttcgca
      301 acattcctac gccagtgcct ggcgcaccgg gaatgtctgt gctgaatatc gatccggaca
      361 cggccgccga atcgtatcga cggcgggtgc tggcgcagat ggacgccagc gcgagcgtca
      421 cggatcgcgc aactgtgcgt gaacaattgt ccggcgcgtg taccacggag atcgcttcat
      481 tcgacgaatt cacctccctg ctgtccgacg gagccggtga ctatgacaat atcgtgtttg
      541 atacggcgcc gacggggcac acgttgcgct tgctcagctt gccgcaggca tggagtggct
      601 ttttgtccgg caatgatcgc ggcgcttctt gcctgggacc gcattcaggt ctaaagatgc
      661 aggaagtgcg tttcaaggct gcaatggaca ccttgaccga tcctggccag acgactgtca
      721 tactcgtcgc ccgcgccgac gggagtgcga ttgccgaggc cgctcgtact tcggaagaat
      781 tgcgtacgct cgggctgaac aaccagcgtc tggtgatcaa tggcgtgttc catgcaagcg
      841 atcctgacga tgtcgtcgct gctgccatca ctacactgga acagcaggcg ttggatgaga
      901 tgccgacatg cctgcaatta ttacctcagg atcgagtgcc gttgcgatcg ttcgacacgg
      961 tcggtctttc tgccctgcgt gcgctgctca cggtcgatgc cagcccgatc gtcagcggga
     1021 atgtgggcac tgaacccatc gagccactgc ccggcctgga cgcgttggcc gacgagttgg
     1081 cgctggccga acacggcttg atcatggtga tgggtaaggg cggggtgggt aagacgacca
     1141 tagccgcagc cttggctctc ggcctgattc aacgtgggaa gaccgtccac ctcagcacca
     1201 cggatccggc agcgcacctt gtcggcaccg tggatgcaga tgtgcctggt ttgcaggtcg
     1261 accgcatcga cccgaagctt gaaacccagc gctatatcga taaggtgatg gcaagcaaat
     1321 cgccggggct taatccgcag gaacaagctt tgctgttgga agattgtcgc tcgccgtgca
     1381 cggaagaagt tgcggttttt catgccttct cgaaaatcgt gaatgaagcc cgtagtgcct
     1441 ttgtcgtgct tgacacagcg ccaaccgggc actccctcct gttgatggac gctaccggtg
     1501 cttatcatcg gcaaacgctg cgcgaattcg gaggccgtgg cgggcgcttg accacgcctt
     1561 tgatgcggct gcaagatgaa acatacacca ggatcatcct ggtgaccttg ccggaggtga
     1621 cgcctgtttc gcaagcagcg gcgttgcaat acgacttgcg ccgcgccggt gtggaacctt
     1681 atgcgtgggt gatcaacaaa agcgtacttg ctgccggcac gcatgatcca ctactggcgg
     1741 cgcggatggc cggcgaacgc aagcagatgc aacgcatggc cgggggattg gccgcacgct
     1801 tgttcgtcgt gccttggcgc gccactccac ccattggcaa tcttgaattg ctcaagctgg
     1861 tcaattaaca gcgcgtcgcc tgcgtggcgc aacgaagtga gagcgctcgg ccagccgggt
     1921 ttcggcggct ggcgctacat acgtgtcgtt cagcgtgcaa tcactgacat tgctgttgca
     1981 tcatttccat atgccgttgg cgcatctcgg gagacatatc ttgcatgttc cgcgccatca
     2041 tcgcttgccg tgcctgtgct gtcggggcgt cgcgcatgcc gcggtacatg gcgcacatcg
     2101 catccttgtc catcatgcgc tgtcctgttg cggaggcgcc ggcgctgtcg cagtttgtca
     2161 ttgggcaggc ggaattgcta ccggtcatgc cgctgcccat cgttccacca gtcgacgccg
     2221 acccgccggc tgccgtatct gctggctgcg ccgtgcgatg ccggccatga ccatggtcgc
     2281 cgtcaggact ggcgcatgcg acaaggccag ccgtcgccac cagcccgagc aggacctgaa
     2341 cagcgggaat ccgttcgatt aacatgttcg ctccttgttc gatagtcgac gtgtttgtcc
     2401 aacatacccc tccatccgtg acgaagcaag gtggacgttg cctgtgccgc cagcgctaac
     2461 gcgtcgaggc ggccaatcaa tgcgacaccg aaatcgtacg atgatggcgg cggaggcctc
     2521 gatacgagat aatcctcggt ttggatggct gcaggatcac gggttcatga ctgattcgag
     2581 tttgaactta gtcaaaagcc aggaacgtaa ttttgagata gtgatgaaat accgcaaacg
     2641 cacattaagt acggcaccca tgatggactg gaccgaccgc cattgccgcg tcttccaccg
     2701 ccagatcacg aaacacacct ggttgtatac agagatggtg acgacgggcg ccctggtcta
     2761 tggcgacgtc gagcgccacc tgcgctacga tgacatcgag catcccatcg cgctgcaact
     2821 gggcggcagc gatccggccg acctcgccaa gagcgcgaag ctgggcgagc agtggggcta
     2881 tgacgagatc aacctgaatt gcggctgccc gtccgagcgc gtgcagaagg gggcgttcgg
     2941 cgcctgcctg atgcaggaac cgcaactcgt cgccgattgc gtgaaggcga tgcgcgacgc
     3001 cgtcagc
//
LOCUS       DATMTW010000075         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_173989, whole genome shotgun sequence.
ACCESSION   DATMTW010000075 DATMTW010000000
VERSION     DATMTW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173989"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2820)
                     /locus_tag="VN084_01490"
     CDS             complement(<1..2820)
                     /locus_tag="VN084_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HWT28156.1"
                     /translation="MTARLREIPYNYTSFSDREIVIRFLGEEIWNVLNELRGERKTGR
                     SARMLFEVLGDLWVVTRNPYLQDDLLANKKRRKALVDALHHRIKAIDERSHGNPKVLK
                     LVIAARQAVTEFENDFKLTENLRKKALAKLGKITRKDNIQFDGLARVSHVTDATDWRV
                     EYPFVVVNPDTEEEVAAIVKACIELGLTIIPRGGGTGYTGGAVPLTKLSAVINTEKLD
                     TLGRVEMRTLPGVSRQVPTIRCGAGVVTRRAMEAATEAGLEFACDPTSADASCIGGNV
                     AMNAGGKKAVLWGTALDNLASWRMVTPDATWMEIERLDHNLGKIHDVDVARFKISRYD
                     ADGKTLHAEPEILEIPGPSFRKIGLGKDVTDKFLSGLPGIQKEGCDGLITSATFILHR
                     MPKHVRTVCLEFFGNVALAVPAIVEIKDYLDATESTLLAGLEHMDERYIKAVGYATKA
                     SRAERPKMVLIADIASDDENAVGEAASQIVRLANARGGEGFIAVSAEARKKFWLDRAR
                     TAAIAKHTNAFKINEDVVIPLPRLGDYSDGIERINIELSIANKLRLLDELQAFFAGDL
                     PLHQDEDTNADAESVEAKRMLSLGLLEQLRTRWTAILNNLDAPVSVLGEEDAKRYGQY
                     ENIFRAVQSYELRVSWKRELKKPLQEIFTGRAYQKILEKCDELQKKILKSRVFIALHM
                     HAGDGNVHTNIPVNSDDYGMMQDAHAAVARVMELAKGLDGVISGEHGIGITKMEFLDQ
                     QTIDTFAAYKQKVDPEGRFNKGKLMAGSGLDSAYTPSFSLLETESIIMEQSAIGEIAD
                     SISDCLRCGKCKPVCTTHVPRANLLYSPRNKILGTSLLIEAFLYEEQTRRGISLKHFD
                     EFNDVADHCTVCHRCLNPCPVDIDFGDVSIAMRNFLREQGKKHFNPGTTLAMTFLNIK
                     DPATIKLMRNFMVGFGYRAQRLGHAV"
     gene            2985..3479
                     /locus_tag="VN084_01495"
     CDS             2985..3479
                     /locus_tag="VN084_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWT28157.1"
                     /translation="MTKWILPIAILGLLVFLGISLANRDKAPQVTFTTITGKTIPLAS
                     LNGQVVLVNFWATDCPGCIAEMPGLIKTYNDYHDKGFEVVAVAMSYDPPSQVLNYSQK
                     NALPFPVMHDGYGEIAKQFNEVRVTPTSYILDKQGKVLGKVVGELDFAALRKLLDEQL
                     RKQA"
     gene            3479..>3837
                     /locus_tag="VN084_01500"
     CDS             3479..>3837
                     /locus_tag="VN084_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWT28158.1"
                     /translation="MLWIKAWHIIFMVTWFAGLFYMPRLFVYHAMADDEVSKERFKIM
                     ERKLFYGIMTPGGVLTIVFGLWLWLGYGISGDWLMAKLLLVAGLVAYHGYCGVLMTDF
                     KHDRNQHSHVYFRWFNEV"
BASE COUNT          753 a         1076 c         1183 g          825 t
ORIGIN      
        1 caccgcatgg cccaggcgtt gtgcccggta accgaaaccg accatgaagt tgcgcatgag
       61 cttgatcgtg gccggatcct tgatgttgag gaaggtcatg gccagcgtcg tgcccgggtt
      121 gaagtgcttc ttgccttgtt cccgcaggaa gttgcgcatg gcgatggaca catccccgaa
      181 gtcgatatcg accgggcatg ggttcaggca gcggtggcag accgtacagt gatcggcgac
      241 atcgttgaac tcgtcgaaat gcttgagcga gatgccacgg cgcgtctgct cttcgtaaag
      301 gaaggcttcg atcagaagtg aagtgcccag gatcttgttg cgcggcgaat atagcaagtt
      361 ggcgcgcggc acgtgcgtgg tgcacaccgg cttgcactta ccgcagcgca ggcagtcgct
      421 gatggagtcg gcgatctcac cgatggccga ctgttccatg atgatggatt cagtctccag
      481 caggctgaaa gatggcgtat aggcgctatc aagtcccgag ccggccatga gcttgccctt
      541 gttgaaccgg ccctcaggat cgaccttctg cttgtaggca gcaaaagtgt cgatggtctg
      601 ctggtcaagg aactccattt tggtgatgcc gataccatgc tcgccggaaa taacgccatc
      661 gagtcctttg gcaagctcca tgacgcgagc cacggcagcg tgcgcgtcct gcatcatgcc
      721 gtagtcgtcg gaattgaccg ggatattggt gtgcacgttg ccgtcgccgg catgcatgtg
      781 cagtgcgatg aagacacggc tcttgaggat cttcttctgc agttcatcgc acttttccag
      841 gatcttctgg taagcgcggc cggtgaagat ctcttgtagc ggcttcttca gctcgcgctt
      901 ccaggacaca cgtaattcgt aggattgcac ggcgcggaag atgttctcgt attgaccgta
      961 gcgcttggcg tcctcttcgc ccagcacgct caccggcgca tcaaggttgt tgaggatggc
     1021 cgtccagcgc gtgcgcagct gttccagcag gccgagcgaa agcatgcgct tggcctcgac
     1081 agactcggcg tcggcgttgg tgtcctcgtc ctgatggagc ggcagatccc cagcgaaaaa
     1141 ggcctgcagc tcatccagca gccgaagctt gttggcgatc gacagctcga tgttgatgcg
     1201 ctcgataccg tccgagtagt cgccaagacg cggcaaagga atgaccacat cctcattgat
     1261 cttgaaagcg ttggtgtgct tggcgatggc ggccgtacgt gcgcgatcga gccagaattt
     1321 cttgcgggct tcggcagaga cggcaatgaa gccttcgccg ccacgcgcgt tggcgaggcg
     1381 gacgatctgc gaagcagcct ctccgacggc gttctcgtcg tcgctggcga tatcggcgat
     1441 cagcaccatc ttcggacgct cggcgcggct ggccttggtc gcataaccga ccgccttgat
     1501 atagcgttcg tccatgtgct ccagcccggc aagcagggtc gattcggtcg cgtccaggta
     1561 atccttgatc tcgacgattg caggcaccgc cagggcaacg ttgccgaaga attcgaggca
     1621 gactgtgcgc acatgcttgg gcatgcggtg caggatgaag gtggccgacg tgatgaggcc
     1681 gtcgcagcct tccttctgga tgcccggcaa cccggacagg aacttgtctg tcacgtcctt
     1741 gccgagaccg atcttgcgga agctcggacc ggggatctcg aggatctccg gttcggcgtg
     1801 cagggtcttg ccatcggcgt cgtagcggct gatcttgaaa cgcgcgacat cgacgtcatg
     1861 gatcttgccc agattgtggt cgagacgctc gatctccatc caggtggcgt cgggggtgac
     1921 catgcgccaa gaggcaaggt tatccagcgc cgtgccccag agtacggcct tcttgccgcc
     1981 tgcattcatg gcgacgttac cgccgataca ggaggcatcg gccgaagtcg ggtcgcaagc
     2041 gaattccagg ccggcttcgg tcgcggcttc catggcgcgg cgcgtcacca cgccggcgcc
     2101 gcaacggatg gtcggcacct ggcgcgaaac gcctggcaag gtgcgcatct cgacacgccc
     2161 cagggtatcg agtttttcgg tgttgatgac ggccgacagc ttggtcaacg gcacggcgcc
     2221 gccggtgtaa ccggtaccgc ctccgcgtgg gatgatggtc agcccgagtt cgatacaggc
     2281 cttgacgatg gcggcgactt cctcctcggt atccgggttg acgacgacaa aaggatattc
     2341 gacacgccag tccgtggcgt cggtgacatg ggacacccgc gcaaggccgt cgaactggat
     2401 gttgtcctta cgggtgatct tgccgagctt ggccagcgct ttcttgcgca ggttctcggt
     2461 gagcttgaag tcgttctcga attcggtgac ggcctgccgg gctgcgatca ccagcttgag
     2521 caccttggga ttgccgtggc tacgctcgtc gatggccttg atgcgatgat gcagggcatc
     2581 aaccagcgcc ttgcggcgtt ttttattggc cagcaggtca tcctgcaggt aggggttgcg
     2641 cgttacgacc cagagatccc cgagcacttc gaacagcatg cgggccgaac ggccggtctt
     2701 gcgctcacct cggagttcgt tgagtacgtt ccagatttct tcgccaagga atcgaatgac
     2761 gatttcacgg tcagagaatg aggtgtagtt gtaggggatt tcgcgtaatc gggcggtcat
     2821 gaatcagctg atggataaag aggccattgt atcacgtgca gtgaccatca agataacatc
     2881 gcggttccgc ctgatagatg acaaatcacg gaaaagacac gacaattgcc ccttttgaca
     2941 gcagtggatt aaccaaggaa aagacgcaac agatgcccag taaaatgacc aaatggattt
     3001 tgccgatcgc catcctcggc ctgctggtgt tcctcggcat atcgctcgcc aaccgcgaca
     3061 aggcgccgca ggtcaccttc accaccatca ccggcaagac cattccgcta gcgtcgctca
     3121 atgggcaggt ggtactggtg aatttctggg cgaccgactg cccggggtgc atcgccgaga
     3181 tgccaggcct gatcaagacc tacaacgatt accacgataa aggcttcgaa gtggtcgccg
     3241 ttgccatgtc ctatgatccg ccgagtcagg tgctcaatta ttcccagaag aacgcgctgc
     3301 cttttcccgt catgcatgac ggctacggcg agatcgctaa gcaattcaac gaggtgcgcg
     3361 tcacgcccac cagctacatc ctcgacaagc agggcaaggt gctgggaaag gtggtcgggg
     3421 agctggattt cgctgcgctg cgcaagttgc ttgatgaaca gctgaggaag caagcctgat
     3481 gctgtggatc aaggcctggc acatcatctt catggtgacc tggttcgcgg gcctgttcta
     3541 tatgccgcgc ttattcgtct atcacgccat ggcggatgac gaagtcagca aagaacgttt
     3601 caagatcatg gaacgcaaac tgttctacgg catcatgacg cctgggggcg tgctgaccat
     3661 cgtctttggg ctctggctgt ggctgggcta tggcatcagc ggcgattggc tgatggccaa
     3721 actgctgctg gtggctggac tggtcgccta tcatggctat tgcggtgtgc tgatgaccga
     3781 cttcaagcat gaccgcaatc agcacagcca tgtgtatttc cgctggttca acgaagt
//
LOCUS       DATMTW010000076         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_476033, whole genome shotgun sequence.
ACCESSION   DATMTW010000076 DATMTW010000000
VERSION     DATMTW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476033"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VN084_01505"
     CDS             complement(<1..887)
                     /locus_tag="VN084_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWT28159.1"
                     /translation="MSAWLIRNLQRVLTGLCLLALGLASANAAAQSRITVLITAPTPA
                     SAEFTDRLRSELEPAGGLSFKIVTEDMLNSAPDAIPEDSLVIAVGVQALLQASKLDLK
                     RSVLGVLVPQPSFDRILVDSKRNPRNFSAVFLDQPYPRQIALIKAILPGTTSLGVLFG
                     PTSLSSSYLLQQAAQAASLGLAQENIYGTDELVPKLRRALESSPVLLAVPDPLVYNRE
                     TAQSVLLTSYRYQKPVIGFSQAYVRAGALAGVFSTPQQIARQTAEVVRQYLNKPQAGL
                     PPPQAPKYFSVDVNHQVARS"
     gene            complement(887..2914)
                     /locus_tag="VN084_01510"
     CDS             complement(887..2914)
                     /locus_tag="VN084_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWT28160.1"
                     /translation="MLCAHPAYAAETASAKLLSEDVYLGDMPNVLTVSRLSQPIADAP
                     SAVTVIDRETIRESGIVDLAEIFRLVPGMYVGTNAGYLQTVNPVVSYHGMTDAYSRRM
                     QVLVDGRSVYEPLYGGVQWSDLPLAIADIERIEVTRGPNAASYGANAFLGVINIITQQ
                     ASEVSGSSVLVTRGSGRSDTFYRYGGRLGDLQYRVTIGHRIDEGLQQRADSKHTDLLT
                     ARADYRASDRDDLEFQLGYNGGSRKEGDPDPQALIFLPRSKEVESHFQQVKWRHSFSA
                     TSDFSLQGYHSYSRSNDPVSSVDLVQFFGPILLNPTLLLDNDVENERYDLEAQHTFSP
                     WQSLRLVWGGSVRLDQITAPFYLNSGDRDSFHLQRIFGHVEWHATPRLVVNAGTLFEH
                     NDLTGSDFSPRASANFRLAPGHTLRIGISTALRTPTYLEEKFMGRFVVPTTIPGVTFI
                     QEQFRDQGGLRPEQIISREIGYLGDFGSLDIDLRVFEDAMSNVIRQTKVASPYSAPPG
                     FIRIPGTPKPFTYINGADARVRGFELQGQWRLSDDTRLQANFAHVRIYGKQERSGPDP
                     LPPEDLLERDFLDSAPNNTLSAMLTHRFNTYWDASLMYYQTSEVAALGDGVPVGLARH
                     ADLRVARKFDAGRFQGEVSAVAQNLFDTHYEEFADYNLMRRRAYLNVRLDF"
     gene            complement(2958..3422)
                     /locus_tag="VN084_01515"
     CDS             complement(2958..3422)
                     /locus_tag="VN084_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWT28161.1"
                     /translation="MELLLWRHAEAADTAPDIQRELTAKGRQQAEIMGKWLRARLPKG
                     SLILVSPATRTQQTAAALRLDFTTRRDLGPDASARTILKAAGWPDADGTVVIVGHQPS
                     LGEAASLALTGKTQYLSVKKGSVWWISSRISEGVTQASLRAVIPPNLIKDPK"
BASE COUNT          644 a         1028 c         1208 g          715 t
ORIGIN      
        1 gaacgggcga cctggtggtt cacatcaact gaaaaatact taggtgcctg tggcggcggc
       61 aatccggcct gtggcttgtt caggtattgg cgcacgactt ccgcggtctg cctggcgatc
      121 tgctgcggcg tactgaacac gccggccagt gcgccagcac ggacataggc ctgcgagaaa
      181 ccgatcacag gtttctggta gcgatagctg gtgagcaaaa cgctttgtgc cgtttcgcgg
      241 ttgtagacca gcgggtcggg caccgccagc agcaccgggc tgctttcgag ggcgcgccgc
      301 agcttgggca ccaattcatc cgtgccgtag atattctctt gcgccaagcc caggctcgcc
      361 gcctgcgccg cctgctgtag caggtaactg gaggaaagcg atgtcggccc gaacaatacg
      421 cccagactcg tggtgccggg caggatagcc ttgatcagcg cgatctggcg cggataaggc
      481 tgatcgagga agacggcgct gaagttgcgg ggattgcgct tgctatcgac caggatgcgg
      541 tcaaatgaag gttggggcac gaggacgccg agcacgctac gcttgagatc gagcttgctc
      601 gcctgcagca gggcctgtac gccgacggcg atgaccagac tatcctccgg gatggcgtct
      661 ggggcgctgt tcaacatgtc ttcggtgacg attttgaagc tcaggccacc cgctggctct
      721 agctcgctcc tcaggcgatc ggtgaattcg gcgctggccg gcgtcggcgc ggtgatgagc
      781 acggtgatgc gggattgcgc cgcggcattg gctgacgcca agcccagcgc cagcagacaa
      841 agcccggtca ggacgcgctg caggttgcga atcagccagg cggacatcag aagtccagcc
      901 tcacgttaag gtaggcgcgg cggcgcatca ggttgtaatc ggcgaattcc tcgtaatggg
      961 tatcgaacag gttctgcgcg acggccgaga cctcaccctg gaagcgcccg gcgtcgaact
     1021 tgcgtgcgac acgcagatcc gcgtgtcgcg ccagcccgac cggcacccca tcgccgagcg
     1081 cagcgacctc gctggtctgg taatacatca ggctggcgtc ccagtaagtg ttgaaacgat
     1141 gcgtcagcat ggcgctcagg gtgttgttcg gggccgagtc gaggaagtcg cgctcaagca
     1201 gatcctccgg cggtagcgga tccgggcctg aacgttcctg cttgccgtag atgcggacgt
     1261 gggcgaagtt ggcttgcaga cgcgtatcgt ctgaaaggcg ccactgccct tgcagctcga
     1321 agccccgcac gcgggcgtcc gccccattga tgtaggtgaa aggtttcggc gtgccgggga
     1381 tgcggatgaa tcccggtggt gcgctgtaag gactggcgac cttggtctgg cggatgacgt
     1441 tgctcatcgc gtcctcgaac acgcgcaggt caatatccag actgccgaaa tcgccaaggt
     1501 agccgatctc cctggagatg atctgctccg gtcgcaagcc gccctggtca cggaactgct
     1561 cctggatgaa ggtcacgccg gggatggtcg tcggcacgac gaatcgcccc atgaatttct
     1621 cttccaggta ggtcggggta cgtagcgcag tcgagatgcc gatgcgtagc gtgtgcccag
     1681 gcgcaaggcg gaagttggcg ctggcgcgtg gtgagaagtc gctgccggtg aggtcgttgt
     1741 gttcgaatag ggtgcctgca ttcaccacca gccggggcgt ggcatgccac tcgacatgac
     1801 cgaatatgcg ctgcaggtgg aaactgtcac gatcaccgct gttgagatag aacggtgcag
     1861 tgatctggtc caggcgcacg ctgccgcccc acaccaggcg cagggattgc caaggcgaaa
     1921 aggtgtgttg cgcctccagg tcgtagcgtt cgttctcgac gtcgttatcc agcagcaagg
     1981 tcggattgag caggatgggg ccgaagaact ggacgagatc gaccgacgac accgggtcgt
     2041 tggaacggct gtagctgtga taaccctgca aggaaaaatc gctggtggcg ctgaagctat
     2101 gccgccactt cacttgctgg aaatggcttt cgacctcctt gctgcggggc agaaagatca
     2161 gggcctgagg atcggggtcg ccttcctttc tgctgccgcc gttgtagccg agctggaact
     2221 cgaggtcgtc acggtcgctg gcgcgataat ccgcgcgtgc cgtgagaagg tcggtgtgct
     2281 tgctgtcggc ccgttgttgc aggccttcgt cgatgcggtg gccgatggtg acgcgatact
     2341 gcaggtcgcc gagtcttccg ccgtaacggt agaaggtatc gctgcggccg ctgccgcgcg
     2401 taacgagcac gctggacccg ctgacttccg acgcttgctg cgtgatgatg ttgatgacgc
     2461 cgaggaaagc gttggcgcca tagctcgcgg cattcggccc acgcgtcacc tcgatgcgtt
     2521 cgatgtcggc gatggccaag ggcaggtcgc tccattgcac gccgccatag agcggctcgt
     2581 agaccgaacg cccgtcgacc agcacttgca tgcgccgtga gtaggcatcg gtcatgccgt
     2641 ggtagctgac gacgggattc accgtctgca ggtaaccggc gttggtgccg acgtacatgc
     2701 caggcaccag gcggaagatc tctgcgagat cgacgatccc cgattcgcgg atcgtctcac
     2761 ggtcgatgac cgtcacggcg gaaggtgcgt cggcaatggg ctgggacagc cgggaaacag
     2821 tcagtacatt gggcatgtcg ccgagataga cgtcctcgga gagcagcttg gctgaagcgg
     2881 tttctgctgc ataggcggga tgtgcgcaca gcatcaggca ggcaattaaa tgtctggcgt
     2941 gcatcatcga ccccggatta cttggggtct ttgatgaggt tgggcgggat gacggccctc
     3001 aggctggctt gcgtgacccc ttcactgatg cggctcgaga tccaccagac actccctttt
     3061 ttgacgctca ggtattgtgt cttgccggtg agggcgagag aagccgcttc gcccaggctt
     3121 ggctgatggc cgacgatgac gacagtgccg tccgcgtcag gccagccggc tgccttcagt
     3181 atcgtcctgg cgctggcgtc cgggccgagg tcgcgccgcg tggtaaaatc gagcctgagg
     3241 gctgctgcgg tctgctgggt tcttgtggca gggctgacga ggatcaggct gcctttcggc
     3301 aagcgtgccc gcagccattt gcccatgatc tcggcctgtt gccggccctt ggcggtcagt
     3361 tcgcgctgaa tgtcaggagc tgtgtcggcg gcttcggcat gccgccagag gagcagttcc
     3421 atgaatatta ttctccctgt gatggggtta tcttaaccgt agcctacgct tgacggaagc
     3481 ggcaatcgca tgaatcgcca tctttacctg ttggctgaca gccttgatcc gatcccggca
     3541 gagagcctgg tggcaatcga ggccttggtc gctcgcggtc gcgtggattc ccgac
//
LOCUS       DATMTW010000077         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_375212, whole genome shotgun sequence.
ACCESSION   DATMTW010000077 DATMTW010000000
VERSION     DATMTW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375212"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VN084_01520"
     CDS             complement(<1..1105)
                     /locus_tag="VN084_01520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT28162.1"
                     /translation="MATLNELFQTAYQLHRNGQYAQAESAYRQLIRQQPRHADALHLL
                     GVLCHQTARHEEAATLIARAIAIDPDNADFLNNCGLALRAAGRAQDAITCYEEAMRLS
                     PKDADIQNNLGNAYQEAGRHAEAANCYRRVLRSHPQDADLRQALVSVLMAEGDQHFAN
                     GRFGDAEHCYQEAVTLAPDTAILHYNLGNAKRELGKAAEAAVLYKRYLALAPTDADGW
                     NNLGNALRELGRLDEAVTAYRQALEHNPTLYHAKVHLVHQQQHMCDWQGLDESVACIR
                     DWVRTKPEALVSPFAFLAMPGTTAAEQRLCALQWARNRHQSLIELGRNLAFNYVKTPG
                     RKLHIGYLSGDFRLHPLAFLITEMIELHDGEKFE"
     gene            complement(1121..>2036)
                     /gene="mnmC"
                     /locus_tag="VN084_01525"
     CDS             complement(1121..>2036)
                     /gene="mnmC"
                     /locus_tag="VN084_01525"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HWT28163.1"
                     /translation="DERVRCEAVAARRLPETLVHYVDAETASDIAGLPLQHSALYFPK
                     GGWVDPVAFCQALVTQPGIRLLTHASALELKRQGDHWQVRDHRQVLAEASIVIIACAN
                     QTASFSQTRHLPLTPVRGQITMVEASERSKQLRAVICSDGYVSPAHQGRHCLGATFSP
                     RESALDERDSDHQANLAMLRRLCPELHQAIDPTRLSGRVALRAAMPDYLPMAGPLLDS
                     EALLADPPTHRTDPADLPWLDGLYINAGHGAKGLLNAPLCAEMLASALCGEPAPVDSQ
                     LLAALDPNRFLLRKMGLKRLVAGLAARP"
BASE COUNT          341 a          624 c          678 g          393 t
ORIGIN      
        1 tctcgaactt ctcgccgtcg tgcagttcga tcatctcggt gatcaggaag gccagcgggt
       61 gtagccggaa atcgcccgaa agatagccga tatgcagctt gcggccgggc gtcttgacgt
      121 aattgaaagc gaggttacgt ccgagttcga tgagcgattg atgccggttc ctagcccatt
      181 gcaaggcgca gagtctctgc tccgcggccg tcgtcccggg catcgcaagg aacgcgaacg
      241 gcgagaccag ggcctccggc ttcgtgcgca cccagtctcg gatacacgcg acgctctcgt
      301 ccagcccttg ccagtcgcac atatgctgtt gctgatgcac caggtgcacc ttggcatgat
      361 agagcgtcgg gttgtgctcc agcgcctgcc ggtaggcggt gacggcctcg tccagccggc
      421 cgagctcgcg cagggcgtta ccgaggttgt tccagccatc ggcgtcggtc ggcgctagcg
      481 ctagatagcg tttgtagagc actgcggcct cggcagcctt gcccagctcg cgtttggcgt
      541 tgcccaggtt gtagtgcagg atcgccgtgt caggcgccag ggtcactgct tcctgatagc
      601 aatgctctgc gtcgccgaaa cgcccgttgg cgaagtgctg gtcgccctcg gccatcagca
      661 ccgacaccag cgcttggcgc aaatcggcat cctgcggatg tgaacgcagt acgcggcgat
      721 agcaattcgc ggcctctgca tgacgaccag cctcctggta ggcgttgccc aggttgttct
      781 ggatatcggc atccttgggc gatagccgca tcgcctcttc gtagcaggtg atcgcgtctt
      841 gcgcgcggcc tgcggcacgc agggccaggc cgcaattgtt gaggaaatcg gcattgtccg
      901 gatcgatggc gatggcgcgc gcgatgagcg tcgcggcctc ttcgtggcgt gccgtctggt
      961 ggcacagcac gcccagcagg tgcagggcgt cggcgtgcct gggttgctgg cggatcagtt
     1021 gccggtaagc ggattctgcc tgggcatatt gcccgttacg atggagttga taagcggtct
     1081 ggaagagttc gttcagcgtg gccatgcccc tgattctacc tcaaggccgc gcggcaagtc
     1141 ccgccacgag ccgcttgagg cccatcttgc gtaacaggaa acgattcgga tcgagcgccg
     1201 ccaggagctg gctgtcgacg ggcgccggct cgccacagag cgcgctggct agcatctccg
     1261 cgcacaaggg cgcgttgagc agacccttgg cgccatgacc agcattgatg tataggccgt
     1321 ccagccaggg caggtcggca gggtcggtgc gatgggtcgg cggatcggcg agcaacgcct
     1381 cgctatccaa tagcgggccg gccatcggca ggtagtctgg catggcggca cgcaaagcga
     1441 ccctcccgct aagccgggtt ggatcgatcg cctgatgaag ttcagggcag agcctgcgca
     1501 gcatcgccag attggcctga tgatcgctgt cgcgctcatc cagcgcggat tccctcggtg
     1561 agaaggtcgc tcccaggcaa tggcgtccct gatgggcggg gctgacgtag ccgtcgctac
     1621 agatcacggc cctcaactgc ttgctgcgct cgctggcttc taccatcgtg atctggccgc
     1681 gcaccggcgt cagcggcaga tggcgtgtct gggaaaagct cgccgtctgg ttggcgcagg
     1741 cgatgatgac gatcgaggct tctgccaata cctgtcggtg atcccgcact tgccaatgat
     1801 cgccctgacg ctttagctcg agtgcgctgg cgtgcgtcag cagacggata ccgggttgcg
     1861 tgaccagcgc ctgacaaaac gccaccggat cgacccagcc gcctttgggg aaataaagcg
     1921 ccgaatgttg cagtggcagg ccggcgatgt cgctggcggt ttcggcgtcg acatagtgga
     1981 ccagcgtttc cggcaagcga cgtgccgcca ccgcttcgca gcggacgcgc tcgtca
//
LOCUS       DATMTW010000078         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_610681, whole genome shotgun sequence.
ACCESSION   DATMTW010000078 DATMTW010000000
VERSION     DATMTW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610681"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VN084_01530"
     CDS             <1..166
                     /locus_tag="VN084_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28164.1"
                     /translation="ILTRPLGAVIGDFLDKPVSAGGLALSRYSASAALLTFMVVAILL
                     FRQRAAKTAH"
     gene            189..854
                     /locus_tag="VN084_01535"
     CDS             189..854
                     /locus_tag="VN084_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWT28165.1"
                     /translation="MRVLLVEDDLMIGEEIQGALKDASCAADWVHDGQTALTTMGCQH
                     YDLVLLDLGLPGKDGLEVLASIRARDNPIPLLIITARDGLDDRLRGLDGGADDYVSKP
                     FHMAELLARIRAVLRRKGGTAAPLLSNGVVTLDPATKDAWVNGGSQVQLSNREFSMLQ
                     ALMVRPGAILSRAELEDRIYGWGDEVESNAVEYLIHALRRKLGSEVIKNVRGLGWMVS
                     KNV"
     gene            830..2206
                     /locus_tag="VN084_01540"
     CDS             830..2206
                     /locus_tag="VN084_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28166.1"
                     /translation="MDGFKKRLNESVQLKLSFTLSLAILAVAVVAGVFSFLSAFDEAH
                     ELQDDVLRQVAQLMDRQRLSPVAPSIDTRSKDADEESRVIVQRLGEANPSGASVDAGG
                     TLSLPTTLIDGLHTLEVSGETFRVLVKTTATGDRIAVAQESGFRNEIARGGALRTVMP
                     FLILMPVLLLIVADLVRKMFQPIAALSKEIDQRAEQEMHPVEEHHLPAEVRPFVVAIN
                     RLLARVGQSMESQRRFLADAAHELRSPLTALSLQAERLAEAEMSDLAHQRLVVLRRGI
                     ERGRNLLDQLLTLAKAQSAGHLPKSAVSVQGVYRRVLEDLMPLAEAKHIDIGLEGMQD
                     AEVWVSEMDIIALVKNLIDNAIRYTPQGGRVDLSVRVSEGSAVLRIQDNGPGIPLAER
                     ERVFDPFYRTLGSKQTGSGLGLSIVQTIANRIGAGIRLDLADVHKQTGLVVTVHFPAI
                     PNLNRYSF"
     gene            2250..>3346
                     /locus_tag="VN084_01545"
     CDS             2250..>3346
                     /locus_tag="VN084_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWT28167.1"
                     /translation="MSGCVAYKPMPLGAVPDPPAHARDIQIDAAKLPFPQLRSHRFDP
                     SDGLDETEVAMLAVANNPALRLARDGLALAHAQAFAAGLLPDPQLVLSQGLSNSNTGA
                     DATKSFGLGLNYDANLLIQHVAGVRAARADERKVSLTLLWQEWQVVSQARLLFAKLVH
                     GRRLINVLEDTRQLFADRVRRTTEAQARGLLASDVVLPALASLQDVDKQLYDARRQAA
                     QNAKDLSTLLGLEATAAVPLRETSGAFPATIAPDDALADLRRRRPDLLALEAGFESQD
                     QRYRGAVLAQFPALNVGITRARDTGNIYDNTLGVTLSLPFMNRNRGNIAIQQATRQRL
                     YDEYQQRVQASRGEIAALLAQRDMDKKQLDRV"
BASE COUNT          645 a         1007 c         1032 g          662 t
ORIGIN      
        1 catcctgacc cgcccgcttg gggctgtcat aggtgacttt ttagataagc cggtgagcgc
       61 gggtggcctg gcgttgagcc gttattcagc ctcggccgcg ctgctgacct tcatggtggt
      121 ggcaattctg ctgttcaggc agcgagcagc caagacggcg cattgatcaa caactggatg
      181 gaggaggaat gcgggtattg ctggtcgaag acgatctcat gatcggtgaa gagatccagg
      241 gcgcattgaa ggatgcgtcc tgtgccgctg actgggtgca tgatggtcaa actgccctca
      301 cgacaatggg ctgtcaacac tacgacctag tgctccttga tttgggtctg cccggaaagg
      361 atggactgga ggtactcgcc agcatccgtg ccagagacaa cccgatccca ctgctcatta
      421 tcacggcccg tgacggcctc gatgaccgcc tgcggggtct ggacggcgga gcagacgact
      481 atgtgagcaa gccctttcat atggcggagc tgctggccag aatacgcgct gttctgcggc
      541 gcaaaggcgg cactgcggca ccccttctca gcaacggcgt ggtgacgctc gacccagcca
      601 ccaaagatgc ctgggtcaat ggcggctctc aagtgcaact atcaaatcgc gagttttcga
      661 tgctgcaagc cttgatggtt cggccggggg ccatcctttc acgcgccgaa ctggaagatc
      721 gcatttatgg ttggggggat gaggtcgaaa gcaatgcggt ggaatatctg atccacgcgt
      781 tgcggcgcaa actcggcagc gaggtcatca agaacgtcag ggggctggga tggatggttt
      841 caaaaaacgt ttgaacgaat cagttcagct caagctctcc tttaccttat cactggccat
      901 cctcgcagtg gccgtcgtgg cgggcgtctt ctcgttcttg tccgccttcg atgaggcgca
      961 cgaactacag gacgatgtac tgcgtcaggt agcgcaactc atggatcgtc agcgcctgtc
     1021 gccagtcgcg ccgagcatcg atacccgttc gaaggatgcc gatgaggagt cgcgcgtgat
     1081 cgtgcagcga ttaggcgaag ccaatccgtc cggggcaagc gtggatgcag gcggcacact
     1141 ttctctgccg acgacactga ttgatgggct gcacacgctg gaggttagtg gagagacgtt
     1201 tcgcgtgctg gtcaagacta cggcaactgg cgatcgcatc gccgtggctc aagaatccgg
     1261 cttccgcaac gaaattgccc gcggcggggc actgcgcaca gtaatgcctt tcctgattct
     1321 catgccagtg ctgctactga tcgtggctga cctggtgcgc aagatgttcc agcccatcgc
     1381 ggcgctttcc aaggagatcg accagcgggc agagcaggag atgcatccgg tggaagagca
     1441 ccaccttcca gccgaggtgc ggccattcgt cgtggcgatc aatcggcttc tcgcccgcgt
     1501 cggccagtcg atggagtcgc agcgccgctt ccttgcggat gccgcgcatg agctgcgctc
     1561 accactgacc gccctttcgt tgcaagccga aaggctggcc gaagcggaaa tgtccgactt
     1621 ggcacaccaa cggctggtag tcttgcgccg agggatcgag cgcggccgga atttgcttga
     1681 tcaactcctc accctggcaa aggcgcagtc ggccgggcac ttgccgaagt cagcggtatc
     1741 cgtccaggga gtctatcgcc gcgtgctgga ggaccttatg ccactggccg aggccaagca
     1801 catcgacatc ggtctagaag gcatgcaaga cgccgaagtg tgggtgagcg agatggacat
     1861 aatcgccttg gtcaagaacc tgatcgataa cgccatccgc tacacgccac agggcgggcg
     1921 cgttgatctt tcagtgcggg tctcggaagg aagcgccgta ctgcgtattc aggacaatgg
     1981 gcctggcatt cctttggctg agcgggagcg cgtttttgac cctttctacc gcacgctggg
     2041 cagcaagcag acaggttcgg gccttggcct gtccatcgtc cagaccatcg ccaatcgtat
     2101 cggggcggga atccgccttg acttggctga cgtgcacaag caaaccggtc tggtcgttac
     2161 cgtgcacttt ccggcgattc ctaaccttaa ccgctattca ttttaatatg aagcaaagac
     2221 gttacctcgg cgcatgcttg gcgcttgctt tgtccggctg tgtcgcatac aaacccatgc
     2281 cccttggtgc cgtgcccgat ccgccggctc atgcccgcga tattcaaatc gacgcggcga
     2341 aactcccgtt tccgcaactg aggagccatc gcttcgatcc ctccgacggg cttgacgaga
     2401 cggaggtcgc gatgttagcc gtcgccaaca atccggccct gcgacttgcg cgcgacggcc
     2461 tcgcgcttgc ccacgcgcaa gcgttcgcgg ctggcttgct gcccgacccg cagctggtgc
     2521 tgagtcaggg tctttcgaac agcaatactg gcgctgatgc taccaagtcc tttggcttag
     2581 gcctgaatta cgacgcgaat ctcttgattc agcatgtggc cggcgtacgc gccgcacggg
     2641 cagacgagcg caaggtctcg ctgaccctgc tctggcagga gtggcaagtg gtgtcgcaag
     2701 cgcgtttgct gttcgcgaag ctcgtccatg gccgccgttt gattaacgta ctggaagaca
     2761 ctcgccagct gttcgccgac cgcgtccggc gcaccaccga ggcgcaagcg cggggcctgt
     2821 tggccagcga cgtcgtcctg ccggcgctgg caagtctgca ggatgtcgac aagcagcttt
     2881 acgacgcccg tcgccaggcc gcgcaaaacg ccaaggacct gagcacattg cttggcctgg
     2941 aggcgacggc cgctgtcccc ctgcgggaaa cgagcggcgc cttcccggcg accatagcgc
     3001 cggatgatgc cttggccgat ctgcgccgcc gccggcccga cctgctggcc ctggaagccg
     3061 gctttgagtc gcaggaccag cgttaccgag gcgccgtgct ggcgcaattc ccggcgctga
     3121 acgtcgggat tacacgcgca cgcgacaccg gcaacatcta cgacaacacc ctcggcgtga
     3181 cactgtctct gcccttcatg aaccgcaatc gcggcaacat cgcaattcaa caggcgacgc
     3241 gtcagcgcct gtatgacgaa tatcagcagc gcgtccaggc tagccgcggc gaaattgccg
     3301 cgctgctggc gcagcgtgac atggacaaga aacagttgga tcgtgt
//
LOCUS       DATMTW010000079         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_610738, whole genome shotgun sequence.
ACCESSION   DATMTW010000079 DATMTW010000000
VERSION     DATMTW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610738"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..950)
                     /locus_tag="VN084_01550"
     CDS             complement(114..950)
                     /locus_tag="VN084_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWT28168.1"
                     /translation="MRRDLTLRKYLIGLALLLGSMVALAAPTEFRMISLQHRFAKDVL
                     PVIESMVGPEGKVSALDNHLMVTATPEQLEAIEQVVAKLDVARRTWRITIGYDDQHDR
                     KVTSAGVRGRVRAGDIVIGTDAAGQASGAGDVRIEIDQRSQRQATKLTQFLSVTDGEQ
                     AFIRVGQSIPYTQQWLSLTQRHVHLQQSTRFHDITTGFLVRPRSIGNEVELAIAPRIA
                     DLGSTGVVDFQALQTTVRVSPGQWFDLGGTMQSRDEVSRSILEQGDSEDARGRQLMIR
                     ID"
     gene            complement(950..1453)
                     /locus_tag="VN084_01555"
     CDS             complement(950..1453)
                     /locus_tag="VN084_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28169.1"
                     /translation="MTQPIYDVLTPDAVLFGEGLYREATDHVIGLAKHELLIFDHDLG
                     RGDHASTRRFAQLQDFLSRAPGNRLTIVLHETADFASRYPRLFGLLKIYGHTMTVYQT
                     NDQAKVARDSFIVADRQHYLRRFHADQPRFRYASDDAETVHMLCQRFDELLEATTHTV
                     ATHSLGL"
     gene            complement(1457..2572)
                     /locus_tag="VN084_01560"
     CDS             complement(1457..2572)
                     /locus_tag="VN084_01560"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWT28170.1"
                     /translation="MELLGGLTPEAFLRDYWQKRPLLIRGAIPDFAGLLSPDELAGLA
                     CEASVQSRLILRDARRWAIEHGPIDEARFGELPARDWTLLVQEVNHHLPEADALLQRF
                     DFIPYARLDDLMVSYAPDGGGVGPHFDSYDVFLLQGQGERLWRISQQQDLTLEEGAPL
                     RILKHFDTEQEYLLQAGDMLYLPPQVAHWGVAVGECMTYSIGFRAPSAQELASQFLDY
                     LHGKLALHGMYEDPDLTVTGHPAEIDAAMVSRVAKMIAVIDWQEADVADFLGSYLSEP
                     KPHLVFDPPRAMSLTSFRQRLQSKGVRLDGKSRLLYRGQRFYMNGEPVEAGAAELPAL
                     TELADRRCLPGGGTHDAALVARLHDWYSAGMLTFDRH"
BASE COUNT          511 a          806 c          767 g          495 t
ORIGIN      
        1 ctacacaggc gccaaaaaaa ataccactgt tttttgcgcg ctagcccctt gttttatggg
       61 aaaagtcacg tttatcagcg agagcgatca aaaaggcgat cctcaccctg ctttcagtcg
      121 atccgaatca tgagctggcg gcctcgcgca tcctcagaat caccctgttc gaggatgctt
      181 ctgctcacct catcccgtga ttgcatcgtc ccgccgagat cgaaccactg accgggcgag
      241 acccgcaccg ttgtctgcaa tgcctgaaaa tcgacgacac ctgtcgaacc caggtcggcg
      301 atgcgcggcg cgatcgccaa ctccacctcg ttgccgatgc tgcgcggcct gacgaggaag
      361 ccggtggtga tgtcgtggaa tcgcgtggat tgctgcagat gcacatggcg ttgcgtcagc
      421 gaaagccact gctgggtata agggatcgac tggccgacac ggatgaacgc ctgctcgcca
      481 tcggtcacgc tgagaaactg ggtgagtttt gtcgcctggc gctggctgcg ctggtcgatc
      541 tcgatcctga cgtcgccagc ccccgaggcc tggcctgccg catcggtacc aatcacgatg
      601 tcacccgcgc ggacccgtcc acgcaccccg gcactggtca ctttacgatc gtgctggtcg
      661 tcatagccga tcgtgatgcg ccaggtcctg cgcgccacat ccagcttggc gaccacctgt
      721 tcgatcgctt ccagctgttc cggcgtcgcc gtgaccatca aatgattatc gagcgcgctg
      781 accttgccct ctggcccgac catcgattcg atcacaggca ggacatcctt cgcgaaccga
      841 tgttgcagcg agatcatcct gaattctgtg ggtgccgcca gtgccaccat gctccccagc
      901 agcaaagcca gtccgatcag atacttgcgc agcgttagat cacggcgcat cacaagccca
      961 ggctatgagt ggcgacggta tgcgtggtcg cctccagcag ttcgtcgaat cgctggcaca
     1021 gcatgtggac agtctctgca tcatcgctgg cgtagcggaa gcgcggctgg tcggcatgga
     1081 atcgccgcag gtaatgttgg cggtcggcca cgatgaagct gtcgcgagcg accttggcct
     1141 gatcgttcgt ttgatagacg gtcatggtat ggccgtagat cttcagcaac ccgaacaggc
     1201 gcgggtaacg tgacgcgaaa tctgcagtct catgcaatac gatcgtgagg cggttgccgg
     1261 gcgcgcgact cagaaagtcc tgtagctggg cgaaccgacg ggtactggca tgatcgccgc
     1321 ggccgagatc gtgatcgaag atcagcagtt catgtttcgc caggccgatc acatgatcgg
     1381 tcgcctcgcg atagagccct tcgccgaaga gcacggcatc tggcgtcagg acatcataga
     1441 tgggctgcgt catggctcaa tgccgatcga atgtcagcat gccggccgaa taccagtcat
     1501 gcaatctcgc gaccagcgcc gcgtcatgcg tcccgccgcc cggcaggcag cgccgatcgg
     1561 cgagttcggt cagggcaggc agctcggcgg caccagcctc caccggctcg ccgttcatat
     1621 agaaccgttg tccgcggtac agcaatcggc tcttgccatc cagtcgcaca cccttgctct
     1681 gcaatcgttg acggaagctc gtcaatgaca tcgcgcgtgg cgggtcgaac acgagatgcg
     1741 gcttgggttc ggaaagatag ctgccgagga aatccgcgac gtccgcctcc tgccagtcga
     1801 taacggcaat catcttggca acgcgactga ccatcgccgc atcgatctct gcgggatgcc
     1861 ctgtcacggt caggtccggg tcttcataca tgccgtggag cgccagcttg ccgtgcagat
     1921 aatcgaggaa ctggctggcc agctcttgcg cgctaggcgc gcgaaagccg atggaatacg
     1981 tcatgcactc gcccaccgcg acgccccaat gcgcgacctg cggcggtagg tagagcatgt
     2041 caccagcttg cagcaggtat tcttgctcgg tatcgaaatg cttgaggatg cgcagcggcg
     2101 caccttcctc cagcgtcagg tcctgttgct ggctgatgcg ccacaagcgc tcgccttgcc
     2161 cctgcaagag gaacacgtca taggaatcga aatgcgggcc gacgcctccg ccatccggcg
     2221 catagctcac catcaaatcg tcgagccgcg cgtatgggat gaaatcgaag cgctgcagca
     2281 gagcgtcggc ttccggcaga tgatggttca cttcctgcac cagcagggtc cagtcacgcg
     2341 ccggcagttc gccgaatcgt gcttcgtcga tcgggccgtg ctcaatggcc cagcgacgcg
     2401 catcgcgcaa aatgaggcgc gattgcaccg acgcctcgca agcgagaccg gccagttcgt
     2461 cagggctcag caagccggca aaatcgggga tggcgccgcg aatcagcaag ggtctctttt
     2521 gccagtaatc gcgcaggaat gcctctggcg taaggccgcc aagcagctcg atgcaggtg
//
LOCUS       DATMTW010000080         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_409097, whole genome shotgun sequence.
ACCESSION   DATMTW010000080 DATMTW010000000
VERSION     DATMTW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409097"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VN084_01565"
     CDS             <1..373
                     /locus_tag="VN084_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWT28171.1"
                     /translation="NARGSAIAWIFAALTTMEPPIVERSMAVLLESDKSWSEERLALL
                     DQRIRNRLDQLSDRLGRADWLDGAFSAGDLMMVSVLRRLRSSGLLKEYPNLAAYVARA
                     EARPAFQRAFDDQLALFKRGS"
     gene            complement(442..798)
                     /locus_tag="VN084_01570"
     CDS             complement(442..798)
                     /locus_tag="VN084_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28172.1"
                     /translation="MPETVLIVEDEFFIAMDLQLMLEGYGWRVMGPVGTVKDALLLLE
                     TELPSVALLDVNLGRELVTPVAEYLKAHDVPFAVATAYDRPEQYGGEVLAGVPNAGKP
                     TDERRLMAALMQLTGR"
     gene            complement(808..1812)
                     /locus_tag="VN084_01575"
     CDS             complement(808..1812)
                     /locus_tag="VN084_01575"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HWT28173.1"
                     /translation="MNMEDLYRLLRTGHIQAQGIVDTVADPMLVLDASLCVQNASRAF
                     FETFKVDREETIGHPIYKLGNGQWDIPELRRLLADVIPKATAIINFEVEHTFPALGRR
                     TMLLTARTLFHPDGASHTLLLSIVDATDRYRRDMAKDMLFGELRHRMKNLLSVVKAIA
                     HQTTTQDRSAKEYRDVFLGRFSALIEAQELAYAEQDEVGVDRLLNIILAPYADKPEAV
                     VIEPGEAIELGPHMIMSLSLVLHELATNAAKYGALSVPSGQVRVGWQVEDANSKLRLK
                     WVESGGPPVSRPAVAGYGSQLIRSTIVYNLHGEVEQDYAVDGFRTEIVIPLGTAPSSS
                     "
     gene            complement(2153..>2669)
                     /gene="sdhA"
                     /locus_tag="VN084_01580"
     CDS             complement(2153..>2669)
                     /gene="sdhA"
                     /locus_tag="VN084_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWT28174.1"
                     /translation="GSHRPLPKDAADLALGRLDHYRNAKGGSPTAKIRLEMQHTMQKN
                     AAVFRDSALLAEGVTKMSQVNQSLADVGVSDRSLIWNTDLIETLELDNLMSQAVCTMV
                     SAENRKESRGAHAHEDFPNRDDENWMKHTISWFDGWGGKGGNVTLNYRPVHDYTLTDE
                     AEYIKPKARVY"
BASE COUNT          557 a          852 c          715 g          545 t
ORIGIN      
        1 gaacgcgcgc ggtagcgcaa tcgcctggat attcgccgcc ctcaccacaa tggagccgcc
       61 tatcgtggaa cgcagcatgg cggtcctgct ggaaagcgac aaaagctgga gcgaggagcg
      121 cctggccctg ctcgaccagc gcatccgcaa tcggctggac caactgtccg accggctggg
      181 cagagccgac tggctggacg gcgcattcag cgcaggcgac ctgatgatgg tatcggtgct
      241 acgcaggctg cgaagctccg gcttgttaaa ggaatatccc aacctcgccg cctatgtagc
      301 cagagccgaa gcacgccccg ctttccagcg tgccttcgac gaccagctgg cgctgttcaa
      361 aaggggcagt tgatcgggca ggctgatccc gctccaccat atcttgcgcg ccactgcggc
      421 cttgggacat cacatccgca gtcagcgacc agttagttgc atcagcgcgg ccatcagccg
      481 ccgttcgtcc gtcggcttac ccgcattggg caccccagcc agtacctcgc caccatattg
      541 ctcaggcctg tcataggccg tcgccacggc aaaaggcacg tcgtgcgcct tcagatattc
      601 cgctacgggt gtaaccaatt cgcggcccag attgacatcc agcaatgcca ccgacggcaa
      661 ttcggtctct aacaaaagca gcgcatcttt gaccgtgccg acgggaccca tcacgcgcca
      721 cccatagccc tccagcatca actgaaggtc catcgcgatg aaaaactcgt cctcaacaat
      781 caacaccgtt tcgggcacgg atggctctca gctagaggat ggagccgttc ccaatggaat
      841 gacgatctct gtccgaaaac cgtctaccgc atagtcttgt tccacctcgc catggaggtt
      901 gtagacgatg gtggaccgga tcagctggct tccatagccc gctacagcag gccgggatac
      961 cggggggcca ccgctctcta cccatttcag ccgcaacttg ctgttggcat cctcgacctg
     1021 ccatccgacg cggacctgtc cgcttggcac tgacaaggca ccgtatttcg cggcgttggt
     1081 cgccagctca tgcagcacca gactcagcga catgatcatg tgcgggccca gctcgatggc
     1141 ctcgcccggc tcgatcacca cagcctccgg cttgtcggca tagggtgcca ggataatgtt
     1201 cagcagccgg tcaacaccga cttcatcctg ctcggcatag gcaagttcct gtgcctcgat
     1261 cagggcgctg aaacgcccca gaaacacatc gcgatactcc ttggccgatc ggtcctgagt
     1321 ggtcgtctga tgcgctatcg ctttcacgac gctcaacaga ttcttcatcc gatggcgaag
     1381 ttcgccgaac agcatgtcct tggccatgtc ccgacgataa cgatccgtgg catcgacgat
     1441 cgacaatagc agcgtgtggc tagccccatc gggatggaac agcgtgcgcg cggtcaaaag
     1501 catcgtcctg cggcccagcg cggggaatgt atgttcgacc tcgaaattga tgatcgccgt
     1561 ggccttgggg atcacgtccg ccagcagccg acgtagttcg ggaatgtccc actggccgtt
     1621 gcccaactta tatatggggt ggccgatcgt ctcttcccgg tcgaccttga acgtttcaaa
     1681 aaatgcgcgg ctagcatttt gaacgcacag gctcgcgtcc aacaccaaca ttggatcggc
     1741 gacagtatcg acgatacctt gcgcctgaat atgtcctgtc cgcagaaggc ggtaaagatc
     1801 ttccatgttc atcaacacta tcctgtacgt agcgtgtgca aagggcaagc cccggcacca
     1861 tcgctcacta ctccgcaaga cagctattcc gcaattcaac gatgtagtat cgccaatttc
     1921 aggttgaatt cttgatctgt gttatctttg ctcgcgatca tcttgacgat tgcaggcatc
     1981 ttcaaacgca ttgctccaaa tttggggaaa tagaggcaag cccgccaacc atagcttcac
     2041 ggcgagcgat tgccaatcca acagcctcac ccccgaccac ggcaccaccc acaacgaaaa
     2101 agggcggact ttgcagcccg ccctcttccg atgcgatctc aaaaagcgca gatcagtaaa
     2161 cgcgcgcctt gggcttgata tattccgcct cgtcggtgag cgtataatca tgcaccgggc
     2221 ggtagttgag ggtgacgttg ccacccttcc caccccagcc gtcaaaccag ctgatggtgt
     2281 gcttcatcca attttcgtca tcgcgattcg ggaaatcctc atgcgcatgc gcgccacggc
     2341 tttccttgcg gttttccgcg ctgaccatgg tgcagaccgc ctgagacatc aggttatcca
     2401 gctccagcgt ctcgatgagg tcggtgttcc agatcagcga tcggtcggat acgccgacat
     2461 cagccaagct ctggttgacc tgcgacatct tggtgacgcc ctctgccagc agcgcgctgt
     2521 cgcggaacac ggcggcattc ttctgcatgg tgtgctgcat ttccagccgg atcttcgccg
     2581 ttggcgatcc gcccttggca ttgcgataat gatccaggcg gcccagcgcc aagtcagccg
     2641 catccttggg cagcggacga tgcgagcca
//
LOCUS       DATMTW010000081         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_308487, whole genome shotgun sequence.
ACCESSION   DATMTW010000081 DATMTW010000000
VERSION     DATMTW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308487"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VN084_01585"
     CDS             <1..660
                     /locus_tag="VN084_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28175.1"
                     /translation="SFVMTGNSIVTPRKTDVISSTGFAGACSSQITVSGNSTFTGAIC
                     SDPGISPKFTANTTVNSDVLTSTGFVSQNIQPGMYVSGTGIAADTVVESVASNTSLVM
                     SKDATASGASTIRISRLKDWFFDNGWQNYVYYAMSKTTGALTVGARTNVDALLLTTGK
                     AIASTPYTQSKASPQSRSLSCSNADYLDSTENANADNVYDVTSKARKENYNDQMFIVS
                     P"
     gene            663..1394
                     /locus_tag="VN084_01590"
     CDS             663..1394
                     /locus_tag="VN084_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWT28176.1"
                     /translation="MIVAKPTTQGFTLVEMAVVLVILGFVISALLLPVAAQRDQAYRT
                     QTDNQLEIARKALLGFAQTNGRLPCPATDTSAGLEAPIGGSTSAVACTVLVGYLPAGT
                     LGIQPVNAAGYAVDGWENPIRYAVTQSNADGAGGVDFTTTGEMNKVTITALAPDLRIC
                     ADSANCSVTQYLTNNAVAVIYATGKNGPTGVGADEVANLDADAIFYSRIPTDTTATVG
                     VFDDQMVWISPFVLYNAMIEAGQLH"
     gene            1409..2167
                     /gene="moeB"
                     /locus_tag="VN084_01595"
     CDS             1409..2167
                     /gene="moeB"
                     /locus_tag="VN084_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWT28177.1"
                     /translation="MNDNQLLRYSRHILLPQIGYEGQEKLTQSHALIVGAGGLGAPVA
                     LYLAAGGIGQLTICDFDTVDLTNLQRQVIHTTQSVGINKAVSAQHTLQAINPEVAVHT
                     VQAKSTEAQFEALASEVDVIIDCSDNFATRYALNRVCVKLRKPLVSGAAIGFEGQVTV
                     FDMRHDSSPCYHCLFPDTGDEEGLRCAENGVFAPLVGMIGTTQAAEAMKLMLGLGESL
                     QGRLLLLDVQAMEWRSIKLSRDPSCKVCGDARAA"
     gene            complement(2182..2352)
                     /locus_tag="VN084_01600"
     CDS             complement(2182..2352)
                     /locus_tag="VN084_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HWT28178.1"
                     /translation="MGSLIGILILVADVYAIVKIAQSGAEPLKQALWIVGVLVFPVLG
                     FIAWYLAGPGAK"
     gene            complement(2399..3241)
                     /locus_tag="VN084_01605"
     CDS             complement(2399..3241)
                     /locus_tag="VN084_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWT28179.1"
                     /translation="MTEFTFVLLIPVVILIWKGIRIVPQGEEWVVERLGKFNAVLGPG
                     LHVINPIFSKISYKVTTKDIILDIPEQEVITRDNAVILANAVAFIKVSNIQRAVYGIE
                     NFQEAMRNMIQTNLRSIIGGMDLNHALTSRDRIKAELKAAIADEAADWGLTVKSVEIQ
                     DIKPSQNMQHAMEQQASAERERVAVVTRAEGEKQSLILNAEARLEAARRDAEAQEVAA
                     KASAESIRLIAEAVKDNNGSATFLLGDRYIQALQKMSNADNSKIVVMPGDLVSAVKAL
                     VGGK"
BASE COUNT          656 a          964 c          975 g          677 t
ORIGIN      
        1 agctttgtca tgaccggtaa ttcgatcgtc acgcccagga agaccgacgt catcagcagc
       61 accggcttcg ccggggcatg tagttcgcaa atcaccgtca gcggtaacag tacgttcacc
      121 ggcgcgatct gcagtgatcc gggcatctcg ccgaaattca ccgcgaacac gaccgtcaac
      181 agtgacgtat tgaccagtac cgggttcgtc agccagaaca tccagcccgg catgtatgtc
      241 tccggcaccg gcatcgcggc ggatacggtg gtcgagtccg tcgccagcaa cacgagcctc
      301 gtcatgtcca aggacgccac tgccagtggt gcctcgacca tccgtatcag ccggctcaag
      361 gactggttct tcgataacgg ctggcagaac tatgtctatt acgcgatgag caaaaccacc
      421 ggtgcattaa cggtgggcgc cagaaccaat gtcgatgcgt tgctgttgac cacaggcaag
      481 gcgatcgcaa gcacgcctta cacgcaatcc aaggccagtc cccaatcccg aagcttgtcc
      541 tgcagtaacg cggattatct ggacagcacg gagaacgcga atgccgacaa cgtttacgat
      601 gtgacgagca aagccagaaa agagaattac aatgaccaga tgttcatcgt ttcgccctga
      661 atatgattgt cgctaaaccc accacacaag gcttcaccct ggtcgagatg gccgtggtgc
      721 tggtcatcct ggggtttgtc atctctgcat tactgttgcc ggtagcagcg cagcgagacc
      781 aggcttatcg tacgcaaacc gacaaccagc tcgagatcgc gagaaaggcg ttgcttggct
      841 tcgcccagac caacgggcgt ctgccttgtc cggcgaccga caccagcgct ggcctggagg
      901 caccgatcgg cggcagtaca tccgccgtgg cgtgtaccgt cctggtgggc tatctgcctg
      961 cagggacttt gggcatccag ccggtcaatg ccgcaggcta tgcggtcgat ggctgggaga
     1021 acccgatccg ttatgcagtc acacaaagca atgcggatgg cgccggtggg gtcgatttca
     1081 cgacgacagg cgagatgaac aaagtgacga tcacggcatt ggcgcctgac ctacgcatct
     1141 gcgcggattc cgcgaattgc tcggtcacgc aatacctgac gaacaacgcc gtcgccgtta
     1201 tctatgccac tggcaagaac ggacccaccg gggtcggtgc cgatgaagtc gccaacctgg
     1261 atgcagatgc catcttctac agccgtatcc cgaccgatac cacagcgaca gttggcgtct
     1321 ttgacgacca gatggtctgg atctcgccat tcgtcctcta taacgccatg atcgaggccg
     1381 gccagctgca ctgagttaga atcgtgtcat gaacgataac cagcttttac gttacagccg
     1441 ccatatcctg ctgccgcaga tcggttatga aggtcaggag aagctcaccc agagtcatgc
     1501 attgatcgtc ggcgcgggtg gcctcggtgc gccggtggcg ctttacctcg ccgcgggcgg
     1561 tatcggccag ctcacgatct gtgatttcga tacggtggac ctcaccaacc tgcagcgaca
     1621 ggtgatccat accacgcagt cggtgggcat caacaaggcg gtctctgcac aacatacctt
     1681 gcaggccatc aacccggaag tggcggtgca caccgtgcag gccaagtcga ccgaagccca
     1741 gttcgaggct ttggcgtccg aggtcgacgt catcatcgac tgtagcgaca atttcgccac
     1801 gcgctatgcc ctgaaccgtg tttgcgtgaa attgcgcaag ccgctggtgt caggcgccgc
     1861 tatcgggttt gaggggcagg tgacggtgtt cgacatgcgt cacgacagca gcccgtgcta
     1921 tcactgcctg tttcccgata cgggcgacga ggaggggttg cgttgcgcgg agaatggggt
     1981 gtttgcgccg ctggtgggca tgatcggcac cacgcaggcg gctgaagcca tgaaacttat
     2041 gcttggcctg ggcgagagcc tgcagggccg gctgttgttg ctcgacgtgc aggcgatgga
     2101 atggcgcagc atcaaattga gccgcgaccc ttcatgcaag gtctgcggcg acgcgcgcgc
     2161 cgcctgagca aaccagtctg actatttggc gccggggcca gccaggtacc aggcgatgaa
     2221 gcccagcacc gggaatacca gcacgccgac gatccatagc gcttgcttca gcggttcagc
     2281 gccgctttgc gcgatcttga cgatggcgta gacatccgcg accaggatca ggattccgat
     2341 aagactgccc atgcttgctc cttgagttat acgttgacgg aatgggttgg cgatcgcatc
     2401 acttgcctcc gaccagtgcc ttgacggcgc tgaccagatc gcccggcatg accacgatct
     2461 tgctattgtc ggcgttcgac atcttctgca atgcctggat atagcggtcg ccaagcagga
     2521 aggtggcgga accattgtta tccttcaccg cctcggcgat cagccggatc gactcggccg
     2581 aagccttggc ggcgacctcc tgtgcttcgg cgtcgcggcg cgcagcttcc agccgcgcct
     2641 cggcgttgag gatcagggat tgcttctcac cctcggcccg cgtcaccacc gcgacgcgct
     2701 cgcgttcggc ggaggcttgc tgctccatcg catgttgcat gttctgcgag ggcttgatgt
     2761 cctggatctc gaccgatttc acggtcaggc cccaatccgc ggcttcatcg gcgatggcgg
     2821 ctttcagttc ggccttgata cggtcgcgcg aggtcagggc gtggttaagg tccatgccgc
     2881 cgatgatgga gcgcaggttg gtctggatca tgttgcgcat cgcttcctgg aaattctcga
     2941 tgccatagac ggcacgctgg atgttgctga ccttgatgaa cgcgaccgcg ttggccagga
     3001 tcactgcgtt gtcgcgcgtg atgacctcct gctctgggat gtcgaggatg atgtccttgg
     3061 tggtgacctt gtagctgatc ttgctgaaga tcggattgat gacatgcagg cccggcccaa
     3121 gcacggcgtt gaatttgcct agccgctcca cgacccattc ctcgccttgc ggcacgatgc
     3181 ggataccctt ccagataagg atgacgacag gtatcagtaa tacgaaggtg aattcagtca
     3241 tgcgggttcc cctgggttaa gtgtttttct gt
//
LOCUS       DATMTW010000082         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_208181, whole genome shotgun sequence.
ACCESSION   DATMTW010000082 DATMTW010000000
VERSION     DATMTW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208181"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="mutL"
                     /locus_tag="VN084_01610"
     CDS             complement(<1..737)
                     /gene="mutL"
                     /locus_tag="VN084_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWT28180.1"
                     /translation="MTSIRLLPDQLISQIAAGEVVERPASALKELLENSLDAGSSEIN
                     VALLNGGIKQLRVADNGRGIAEDDLTLALTRHATSKIATLEDLEMVGSLGFRGEALAS
                     IASVSRTQLISREATGKHAWRITSEGSDLSALEPAALDKGTIVEVQDLYFNTPARRKF
                     LKTEATEFGHCEEAFHRIALSRPDVTFLLQHNGRVLARLAAGEPERRFGEVLGDDFAA
                     EAFFLDESAGGLRLWGMAARPTFSRHAR"
     gene            complement(800..1819)
                     /locus_tag="VN084_01615"
     CDS             complement(800..1819)
                     /locus_tag="VN084_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HWT28181.1"
                     /translation="MKAIGLTRYLPINDPDSLQDLELPKPTAAGHDLLVRIHAIAVNP
                     IDTKVRKPKDIVEATPRILGWDAAGEVAAVGPDCSLFKPGDRVYYSGDVTRQGCNAEY
                     QLVDERIVGHMPRSLHYEQAAALPLTSVTAWESLFDRLGIITDLAHNQGRSILIIGAA
                     GGVGSIAIQLAKQVAGLTVVATASRPSTVTWVEGLGADHVVDHSQPLVAQMASMGLPQ
                     VDYIFCCSHTDAYFESMVTLIKPQGRICAIVDAMQPLNMTLLKPKSAGFVWESMFTRS
                     MFKTPDMIAQHHILERVAALIDDRTLQTTLNEVISPINAANLRAVHAKLESGSTIGKV
                     VLSGW"
     gene            complement(1916..2521)
                     /locus_tag="VN084_01620"
     CDS             complement(1916..2521)
                     /locus_tag="VN084_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWT28182.1"
                     /translation="MGDELHIGKAADTRQQILHTARGIILGKGFAAVGLNEILSCAGV
                     PKGSFYHYFKSKEQFGNAMLEDYFEEYMAMLETLLGDKTLPAGIQLLGYFRYWLASQT
                     SDTTEEKCLIVKLSAEVTDLSESMRLTLKRGTDGIIRRLAQAVEAGVREGEFPADIEV
                     TRVTHELYYLWLGATLLTKVQRNRDALENAYRATCERLGTA"
     gene            complement(2648..>3150)
                     /locus_tag="VN084_01625"
     CDS             complement(2648..>3150)
                     /locus_tag="VN084_01625"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWT28183.1"
                     /translation="VSIHADAFTRPEARGSSVFALSERGATSASARYLAKKENESDLI
                     GGVSLDDKDPYLARTLLDLSQTATINDSLKLGKAVLGHIGEINTLHKNSVEQAGFAVL
                     KSPDIPSILVETAFISNPEEERRLNDEAYQQQLAKAILSGIKRYFSSNPPLARSKLAL
                     DQSSAD"
BASE COUNT          586 a          927 c         1007 g          630 t
ORIGIN      
        1 cgcgcatggc gggaaaaggt cggccgcgcc gccatgcccc acaggcgcaa gccgccagca
       61 gattcatcga ggaagaaggc ctcggcggcg aagtcatcgc ccagcacctc accgaaccgg
      121 cgctccggtt cgccagcagc cagccgtgcc agcacgcgcc cattgtgctg tagcagaaac
      181 gtgacatcgg gacgcgacaa ggcgatgcgg tgaaacgcct cttcgcaatg cccgaactcg
      241 gtggcttcgg tcttgaggaa cttacgccgc gccggcgtgt tgaagtaaag gtcttgcacc
      301 tcgacgattg tgcccttgtc gagtgctgcg ggctccagcg ccgaaagatc gctgccctcg
      361 gacgtgatgc gccaggcatg cttgccggta gcttcgcggc taatgagctg ggtgcgggag
      421 acagaggcga tgctggcgag cgcctcgccg cggaagccga ggctgccgac catttccagg
      481 tcttccagcg tggcgatctt gcttgtggca tggcgcgtca gcgccaacgt caggtcgtct
      541 tcagcgatgc ccctgccgtt atcggcgact cgcaattgct tgatcccgcc attgagcaag
      601 gcgacgttga tctcgctact gccggcgtcg aggctgttct cgagcaattc cttgagcgcg
      661 gaggctggtc gttcgaccac ttccccggcg gcgatctggc tgatgagctg gtctggcagc
      721 aggcggatgg atgtcatgcg gacattttaa cctgctttcg aagctggctg acgcgcgggc
      781 ggattcggag cgagcagcct caccagcctg aaagcacgac cttgccgatg gtgctgccgg
      841 attcgagctt ggcatgcact gcacgcaggt tggctgcgtt gatcggcgaa atcacctcgt
      901 tcagcgtggt ctgtagcgtg cgatcatcga tcagtgcagc gacgcgttcc aggatatggt
      961 gctgggcgat catgtccggg gtcttgaaca tggaccgcgt gaacatcgac tcccacacga
     1021 agcccgcgct tttgggcttg agcagggtca tgttgagcgg ctgcatggcg tcgacgatcg
     1081 cgcagatcct gccctgcggc ttgatgagcg tgaccatgct ctcgaaatac gcatcggtat
     1141 ggctgcagca gaagatgtaa tcgacctgtg ggagccccat gctcgccatt tgggcgacca
     1201 agggctggct gtgatcgacg acatggtctg cgcccaatcc ctcgacccag gtcaccgtgc
     1261 tcgggcggga cgccgtagcg acgacggtga gcccggcgac ctgcttcgcg agctggatgg
     1321 caattgagcc cacgccgcca gccgcgccga tgatgaggat ggatctcccc tggttatgcg
     1381 ccagatcggt gatgatgccg agacgatcga acagcgattc ccaggccgtg accgaggtca
     1441 ggggcaaggc cgccgcctgc tcgtagtgca gagaacgcgg catgtggcca acgatacgct
     1501 cgtcgaccaa ctggtattcc gcgttgcagc cctggcgcgt gacgtcgccc gagtaataga
     1561 cgcggtcacc gggcttgaac aggctgcaat ccggcccaac agctgccacc tcgcctgctg
     1621 catcccagcc gaggatgcgc ggcgtggctt caacgatatc cttgggtttg cgcaccttcg
     1681 tgtcgatggg gttgactgcg atggcatgga tcctgaccag caggtcgtgc ccagccgccg
     1741 tgggcttggg cagctccaga tcctgcagcg aatccgggtc gttgatggga aggtaacggg
     1801 tcagtcctat ggctttcatg atgctccctg tgcacgtctt tgatcgggtt ttactggata
     1861 cgtgtcctgc tgaatattag accagtcgac taaacattgg ccaaaagagt caggttcagg
     1921 cggtgcctag ccgctcgcaa gtggcacggt aagcgttctc cagtgcatcg cgattgcgct
     1981 gcaccttggt caacagggtc gcgcccagcc aaaggtaata cagctcatgc gtcacgcgtg
     2041 tcacctcgat atcagcgggg aactcgccct cacggacccc cgcctccact gcctgcgcca
     2101 gccgacggat gatgccgtct gtcccacgtt tcaaggtcag ccgcatggat tcggaaaggt
     2161 cggtgacctc ggcactgagc ttgacgatca ggcacttctc ttcggtggta tcgctggtct
     2221 gtgacgccag ccagtagcga aagtagccga gcaactgaat gccggcaggc agggtcttgt
     2281 cacccagcag ggtctccagc atggccatgt attcctcgaa gtaatcttcg agcatggcgt
     2341 tgccgaactg ctccttggat ttgaagtaat gatagaacga acccttgggc acgcctgcgc
     2401 aggacaatat ctcgttcagc ccgacggccg cgaacccttt gccgagaatg atccctctgg
     2461 cggtatggag gatctgctgc cgtgtgtctg cggccttgcc gatgtgaagt tcgtcaccca
     2521 tgcgatgtag tctgacatag cgccagacgc accgtccagc aaacgcgatg atcgtgaccg
     2581 tgcgtggcca ggaaacacct gaagaagccc ggcgctaccg aaaaacaggt atggcgcggg
     2641 cgcatggtca gtctgcggaa gattgatcga gcgcgagttt agagcgcgct agcggcggat
     2701 tgctggagaa ataacgcttg atgccagaga ggatggcctt ggcgagctgc tgctggtagg
     2761 cctcgtcatt gagccggcgc tcttcttccg gattactgat gaacgccgtc tccaccagga
     2821 tggaggggat gtccggcgat ttcagcacgg cgaagccggc ctgctcgaca ctattcttgt
     2881 gcagggtgtt gatctcgcct atgtgcccca gcacggcttt gccaagcttc aggctgtcgt
     2941 tgatggtcgc ggtttgcgac aggtcgagca gcgtgcgggc aaggtatggg tccttgtcat
     3001 cgagactcac gccaccgatc agatccgatt cgttctcctt cttcgccagg tatcgcgcgg
     3061 aggcactggt ggcaccgcgc tcagacaagg cgaagaccga ggagccgcgg gcctctgggc
     3121 gcgtgaaggc atcggcgtgg atggagacga
//
LOCUS       DATMTW010000083         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_108020, whole genome shotgun sequence.
ACCESSION   DATMTW010000083 DATMTW010000000
VERSION     DATMTW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108020"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3180)
                     /locus_tag="VN084_01630"
     CDS             complement(<1..3180)
                     /locus_tag="VN084_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HWT28184.1"
                     /translation="MTSSTKHAPDAPQRRELTPSTLDFPVVGIGASAGGLQAVKSFFQ
                     HMPDDSGMAFVVILHLSPDHHSIVDRIIQDVTAMPVLQVTETTDIKKNTVYVIPPAHH
                     LAMSDGHLSVTSTDRHPGLNIAIDLFFRDLADVHKERAFCLVLSGTGSDGAVGLSRIK
                     EQGGVTLVQAPEDAEFDGMPRAAIDTRMVDLVLPVVQMPEKLLELWRNSQAIRLPNAN
                     DPDASIGPAPGERDAAVAEQRLQDILLQLRAGTGHDFKHYKRATVLRRIERRLQVTAQ
                     PDLAAYYQYLQAHPQETRELLCDMLIGVTNFFRDREAFQALERDVVPSLLKSLGPQRD
                     EIRVWSAGCSTGEEAYSLAMMLTEQLAQQESPAKLQVFATDIDDRAIAFGRNGVYPEA
                     IATDVPPSYMRQYFSREQQHFRVRKEVREKVLFAKHSLLLDPPFSQIDLIVCRNLLIY
                     LDRDVQRDIMQMFHFALRPGGYLFLGTSESADACLDLFTPVDKRNRIFRAKSGLTHTR
                     RAPAMPRGGYVRTTIASSPVVAQSARKTSYADIHLRALEKAAPPSIIVDANANILHIT
                     EGAGRFLRYVAGELSRNLLTLAHPDLRLEIRTTLFQVQQAGTPATSRKVRIERERRSY
                     LVDLTAHPYKDDETENEYVLVIFEEREVEPQDSLASVSGQTENRMLSNLEQELQRTKL
                     HLQETIEQSEVSSEELKASNEEMQAINEELRSATEELETSKEELQSINEELLTVNYEL
                     KTKVEETDKINDYLSNLITSTDIATVFVDRNMRIRWFTPRATDIFSMLPVDTGRSLMD
                     ITHRLDYPQMAEDAATVFESLNMIEREVSSNDQRWYIARLLPYRSSEDHIDGTVLTFI
                     DISKRRAAEDELRLGEERMRLVAQSTHDFAIIILDEHGVITDWNTGAQLIFGYAKDEV
                     LGACYDLIFTPEDRAEGVPEAELAAARKHGRGEDERWHLRKDGSRFYCSGEVALLKGE
                     SLQGYVKIARDLTGHKRMHDQQSQQLADTQTSSHLKDEFFAVMSHELKHPLNLIQLNA
                     ELLRRLPMTKSAGPAMKAVNTICDAVASQA"
BASE COUNT          558 a          925 c         1042 g          695 t
ORIGIN      
        1 cgcctggctg gcgaccgcat cgcagatggt gttcacggct ttcatcgccg gcccggcgga
       61 tttggtcatc ggcaggcgcc gcagcagttc ggcattgagc tggatgagat tgagcgggtg
      121 cttgagttcg tgggacatca cggcaaagaa ttcgtccttg aggtgactgc tggtctgggt
      181 gtcggccagt tgctggcttt gttggtcgtg catgcgcttg tgaccggtga ggtcgcgggc
      241 aatcttcacg tagccctgca ggctttcccc cttgagcaag gcgacttcgc cactgcaata
      301 gaagcggctg ccgtccttgc gcaagtgcca gcgctcgtct tcgccgcgcc catgtttgcg
      361 tgccgccgca agttcggctt caggcacgcc ctcggcgcgg tcctcgggag tgaagatcag
      421 gtcatagcag gcgccgagca cttcgtcctt ggcgtaaccg aaaatcagct gcgcgccggt
      481 gttccagtcg gtaatcacac catgctcgtc gagaatgatg atcgcgaagt cgtgggtgct
      541 ttgcgccacc agacgcatgc gttcctcacc caggcgcagc tcgtcttcgg cggcgcgccg
      601 cttgctgatg tcgatgaagg tcagcaccgt gccgtcgata tgatcctcgc tggaacgata
      661 gggcagcaat cgggcgatgt accagcgttg gtcgttgctg ctgacctcgc gctcgatcat
      721 gttcagcgat tcgaagacgg tcgccgcgtc ctcggccatc tgcgggtaat cgaggcggtg
      781 ggtgatgtcc atcaacgagc gcccggtgtc gaccggcagc atgctgaaga tgtccgtggc
      841 cctgggggtg aaccagcgga tgcgcatgtt gcgatcgacg aacaccgtgg cgatgtcggt
      901 ggaggtaatc aggttggaaa ggtagtcgtt gatcttgtcg gtttcttcaa ccttggtttt
      961 gagctcgtag ttgaccgtca gcagttcctc gttgatcgac tgcagctctt ccttgctggt
     1021 ctccagctct tcggtagctg aacgcaactc ctcgttgatg gcctgcattt cttcgttgga
     1081 cgccttgagc tcttcgctgg agacctccga ttgctcgatg gtctcctgca aatgcagctt
     1141 ggtacgttgc agctcttgtt cgagattgct caacatccgg ttttcggtct ggccagagac
     1201 gctggcgagc gagtcctgag gctcgacttc gcgctcctca aagatcacca ggacgtattc
     1261 gttttcggtc tcgtcgtcct tgtaggggtg agcggtcagg tcgaccagat agctgcgccg
     1321 ctcacgctcg attcgcactt tgcgcgaggt cgctggcgtg ccggcctgct gcacctgaaa
     1381 cagcgtggtg cggatctcca gtcgcaggtc cggatgggcc agcgtcagca agttgcgcga
     1441 caactcgccc gccacataac gcaggaagcg cccagcacct tcggtgatgt gcaggatatt
     1501 ggcattggca tcgacgatga tgctcggcgg cgcggccttt tccagggcgc gcaaatggat
     1561 atcggcatag gacgtcttgc gtgcggattg agcgacgacg gggctgctgg cgatggtcgt
     1621 gcgcacgtag ccaccgcgag gcatggccgg tgcccgtcgg gtatgggtca ggccactctt
     1681 ggcgcgaaag atgcggttgc gtttgtccac gggggtgaac aggtccaggc aagcgtcagc
     1741 ggactcggag gtccccagga acaggtagcc gcccgggcgc aaggcgaaat ggaacatttg
     1801 catgatgtcg cgttgtacgt cgcggtcgag gtaaatcagc aggttcctgc agacgatcag
     1861 gtcgatttgc gaaaacggcg ggtccagcaa caggctgtgc ttggcgaaca gcactttctc
     1921 gcggacttcc ttgcgcacgc gaaagtgctg ttgttcgcgg ctgaagtact ggcgcatgta
     1981 tgacggcggg acgtcggttg cgatcgcttc gggatagacg ccgttgcggc cgaaggcgat
     2041 ggcccggtca tcgatatccg tggcgaagac ttgcagcttg gcggggctct cttgctgcgc
     2101 cagctgttcg gtcaacatca ttgccaggct ataggcttct tcgcccgtcg agcagcctgc
     2161 cgaccagacg cgtatctcgt cacgctgtgg ccccaacgat ttgagcaggc tcggtacgac
     2221 atcgcgctcc agcgcctgga aggcttcgcg gtcgcgaaag aaattggtca cgccgatcag
     2281 catgtcgcac agcagttccc tggtttcttg cggatgagcc tgcaggtact ggtaataggc
     2341 ggccagatcc ggttgcgcgg tcacttgcag gcgccgctcg atgcggcgca acaccgtcgc
     2401 gcgcttgtag tgcttgaagt catggccagt gcctgcccgc agttgtagca gtatgtcctg
     2461 cagccgttgt tccgcgacgg cggcgtcgcg ctcgccggga gccgggccga tactggcgtc
     2521 aggatcgttg gcgttcggca ggcgaatggc ttgcgagttg cgccatagct ccagcagctt
     2581 ttccggcatt tgcaccaccg gcagcaccag gtcgaccatc cgggtgtcga tggcggcgcg
     2641 aggcatgccg tcgaactcgg catcttcggg tgcctgcacc aacgtgacgc cgccttgttc
     2701 cttgattcgc gacaacccca ccgcgccatc agaaccggtg cccgaaagca ccaggcaaaa
     2761 ggcgcgttcc ttgtgcacat ccgccaggtc cctgaagaac aggtcgatgg cgatgttcaa
     2821 gccagggtgt cgatcggtcg atgtcaccga caggtggcca tcgctcatcg ccaggtggtg
     2881 cgcgggcggg atgacgtaga cggtgttctt cttgatatca gtcgtttcgg tcacctgcag
     2941 caccggcatc gcagtgacat cctgaatgat ccggtcgacg atgctgtggt gatccggtga
     3001 caaatgaaga atcaccacga aggccatgcc gctgtcatcg ggcatgtgtt gaaaaaatga
     3061 cttcacggcc tgaagcccgc cggcggaagc gccgatgcct accacaggaa agtccagggt
     3121 actgggggtc aactctctgc gctggggggc atcgggtgcg tgcttcgtgg aagacgtcat
     3181 acattaaggc cttaaatggc gaccgcagct gcctgcccac
//
LOCUS       DATMTW010000084         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_241711, whole genome shotgun sequence.
ACCESSION   DATMTW010000084 DATMTW010000000
VERSION     DATMTW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241711"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1832
                     /locus_tag="VN084_01635"
     CDS             177..1832
                     /locus_tag="VN084_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28185.1"
                     /translation="MIVNYDYRVIALLLLVTFIAALIAFSLSAKEYRRAPTSFGLWFA
                     AGAAAVAAGVWLMSYTGFFSMSLGGSLLAGSIIMLAIWLCIVIVSRFSGRQFSLSNTF
                     KQANYELSRMAMLDTLTQLPNRRAFQQHLDFGIRRSARMGNSIALAFIDLDGFKPIND
                     ALGHHIGDEVLQAVAKRLNTAVRGCDVVARIGGDEFVALLEDIKTDQDIIPIVERMIQ
                     SLRDIFFIDHHEISISASIGIAVYPRDGDIERLMVCADAAMYRAKTDGKNQFRFFDSD
                     IELASDLLMEMQRDLSHALAKDEFRLHFQPKVDSKTRLLTGVEALLRWQHPIKGVLAP
                     NAFIHAAEKFGLINQIGEWVIEESCRTLHRLRAQGLDLRIAINLSPQQCRNPNLVSHV
                     IRVMERFDLPRSALMFEVTELAATTNPEQMDSLLLAFKQAGISVSMDDFGAGNSSLVC
                     LQHLKIDELKLDDVLVKHIAVSDRSRAITSSIIALAHALGLNVVAEGVETEDQRQMLE
                     KLGCDEQQGFLFSRPVPEEKLAALIKQLSGAVLPDSPGPLVAE"
     gene            1903..2253
                     /locus_tag="VN084_01640"
     CDS             1903..2253
                     /locus_tag="VN084_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HWT28186.1"
                     /translation="MKLYGIPNCNTVKKAREWLDTHGVAYTFHDFKKQGIDDATLHAW
                     LVQQPWEKLVNRAGMTWRGPSDDEKAQVKDNASAAALMRTKTSVIKRPVLVSEDRILA
                     LGFDADQYEALTRA"
     gene            2269..>3438
                     /locus_tag="VN084_01645"
     CDS             2269..>3438
                     /locus_tag="VN084_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWT28187.1"
                     /translation="MLDAISRRGSTSLLCLMAAWCLASCATPPKVDAIVNQTPDAGTP
                     TRVVNARGPLTQAEGKALIARSARNHKEADLLQDHLLIEQEIAETPLVAGNDVHLLRD
                     GEQTFNAMFSLIRQARQHIHLEYFIFEDVQHHGESLGDLLIAKRKAGVAIDVIYDGIG
                     SITTPPAFFERLRLAGVQVVTFNPIDLAHAPDINNRDHRKILIVDGESAIVGGINLSL
                     SYESSRLRRSAGVATHRPEHWRDTDLLIRGPAVDQLQRLFIQHWAEQHGPALSEPGLF
                     PDHTNRGKEIVRIIGSSPEDALPRYYVTLLSAIRNAEQSIYLSSAYFVPTEDEKKDLV
                     AAARRGVAVSIFVPGVSDSPAAIRVQHSHYEDLLDAGVKIYESEHEVLHAKTISID"
BASE COUNT          702 a         1109 c          975 g          652 t
ORIGIN      
        1 accgccgcta tccagcacca gaaaatataa tagtcaatcc attgtttttt aacggcattt
       61 gaccatcctc caagcagcta ccagcaggct tgcgtatcgc tttcgcctat gcggcatgca
      121 tattgcgact cactgtatgt atatgaatcc tcatgaccct gatgagaacg gactccatga
      181 tcgtaaatta tgattatcgc gtcattgcgt tgctgctact ggtgaccttt atcgcggcac
      241 tgatcgcctt cagcctatcg gcaaaggaat accggcgcgc gccgacttcc ttcggcctct
      301 ggttcgctgc gggcgcagct gcggtcgccg ccggcgtctg gctcatgtcg tataccggct
      361 tcttcagcat gtcgctcggc ggttccttgc tggcaggctc gatcatcatg ctggcgatct
      421 ggctttgcat cgtcatcgtc agccgtttca gcggaaggca attctcgctg agcaatacct
      481 tcaagcaggc caactacgaa ctgagccgca tggcgatgct ggacacgctg acgcaactgc
      541 cgaatcggcg ggcgttccag cagcatctgg atttcggcat ccgtcggagc gcgcgcatgg
      601 gcaactccat cgcgctggcc ttcatcgacc ttgatggctt caagccgatc aacgacgcgc
      661 tcggtcacca catcggcgac gaagtgctgc aggccgtggc caagcgcctg aataccgcgg
      721 tgcgcggttg cgacgtggtc gcccgcatcg gtggtgacga attcgtcgcg ctactggaag
      781 acatcaagac cgaccaggac atcatcccca tcgtcgagcg catgatccag tcgctgcgcg
      841 acatcttctt catcgaccat cacgagatct cgatctcggc ctccatcggg atcgcggtgt
      901 atccgcgtga cggcgacatc gagcgcctga tggtgtgcgc cgacgccgcc atgtatcgcg
      961 ccaagaccga cggcaagaac cagttccgct tcttcgactc cgatatcgag ctggcgtcag
     1021 acctgttgat ggaaatgcag cgcgacctga gccacgccct ggccaaggac gagttccgcc
     1081 tgcacttcca gcccaaggtc gacagcaaga cgcgcctgct caccggcgtt gaggcgttgc
     1141 tgcgctggca gcatccgatc aagggcgtgt tggcgcccaa tgcgttcatt catgcagcgg
     1201 agaagttcgg gctgatcaac cagatcggcg aatgggtgat cgaggaatcc tgccgcaccc
     1261 tgcaccgtct gcgcgcgcaa ggcctggatc tgcgcatcgc catcaacctg tcgccgcagc
     1321 aatgtcgcaa ccccaatctc gtcagtcacg tgatccgggt catggagcgc ttcgacctgc
     1381 cacgcagcgc gctgatgttc gaggtgacgg aactggccgc gaccaccaac ccggagcaga
     1441 tggattcgct attgctggca ttcaagcagg ctggcatcag cgtctcgatg gatgatttcg
     1501 gcgcaggcaa ctccagcctg gtctgcctgc agcacctcaa gatcgacgag ctgaaactgg
     1561 atgacgtgct ggtcaagcat atcgctgtca gcgaccgctc gcgcgccatc accagctcca
     1621 tcatcgcgct tgcgcacgcg ctgggcctga atgtggtggc cgaaggcgtg gaaaccgaag
     1681 atcagcgcca gatgctggag aaactcggtt gcgacgagca acaggggttc ctgttctcgc
     1741 gcccggtacc tgaagaaaaa ctggccgccc tcatcaagca gctctcgggt gccgtgctgc
     1801 cggatagccc ggggccgctc gtcgccgaat agcccgaaag gcctatcacg cccatcgcat
     1861 ggggctgtta aaatgtgcct gacccatctt taggcacgct cgatgaagct ctacggcatc
     1921 cccaactgca acaccgtgaa aaaagcccgc gaatggctgg atacccacgg tgtcgcctac
     1981 accttccatg acttcaagaa acaaggcatc gacgacgcca cgctgcatgc gtggctggtc
     2041 caacagccct gggagaaact ggtcaatcgc gccggcatga catggcgcgg cccatccgac
     2101 gacgaaaagg cgcaggtcaa ggacaacgcc tctgccgccg ccctcatgcg aaccaagacc
     2161 agtgtcatca agcggcctgt gctggtctcc gaagaccgga tactcgcgct ggggttcgat
     2221 gccgaccaat atgaggcgtt gacccgagcc tagcctcgcg cgctggccat gcttgacgcc
     2281 atctcacgcc ggggttccac cagtctgctc tgcctgatgg cagcgtggtg cctcgcgagt
     2341 tgcgccacgc cacctaaggt cgatgccatc gtcaaccaga cacccgacgc cggcacaccg
     2401 acacgggtcg tcaacgctcg cggccccctg acgcaagcgg agggtaaagc gctcatcgcc
     2461 aggagcgccc gtaaccacaa ggaagccgac ctgctgcagg atcacttgtt gatcgagcag
     2521 gagatcgccg aaacgccact ggtcgccggc aatgatgtgc acctgctgcg cgatggcgag
     2581 caaaccttca atgccatgtt ctccttgatc aggcaggcac gccagcatat ccatctcgaa
     2641 tatttcatct ttgaggacgt gcagcaccac ggcgaatcac tcggcgacct gctgatcgcc
     2701 aaacgcaagg caggcgtcgc gatcgacgtc atctacgacg gcatcggctc catcacaacg
     2761 ccgcctgcct tcttcgaacg tctcagattg gccggcgtgc aggtcgtgac gttcaacccg
     2821 atcgatctcg cgcatgcgcc ggacatcaac aatcgcgatc atcgcaagat cctgattgtc
     2881 gacggcgaat ccgctatcgt cggcggcatc aacctcagcc tgagttacga atcatctcgc
     2941 ctgcgccgtt ccgccggggt cgccacgcac aggcccgaac attggcggga tacggacctg
     3001 ctcatccgcg gaccggcagt cgaccagttg cagcggctct tcatccagca ctgggcggag
     3061 cagcatggcc ctgcgctgag tgagcccggc ctctttcccg accacacgaa ccgcggcaag
     3121 gagatcgtgc gcatcatcgg cagctcgccc gaggatgcac tgccgcgcta ttacgtgacg
     3181 ctgctgtcgg cgatccgcaa cgcggaacaa agcatctatc ttagctcagc gtatttcgtg
     3241 ccgaccgagg acgagaaaaa agacctggtc gctgcggcgc ggcgcggtgt ggcagtcagc
     3301 atcttcgtgc ccggcgtcag cgactcgcct gccgccatcc gtgtccagca ttcacattac
     3361 gaggatctgc tcgatgcagg cgtcaagatc tacgagagcg agcacgaagt gctgcatgcc
     3421 aagaccatca gtatcgat
//
LOCUS       DATMTW010000085         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_342750, whole genome shotgun sequence.
ACCESSION   DATMTW010000085 DATMTW010000000
VERSION     DATMTW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342750"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VN084_01650"
     CDS             complement(<1..1524)
                     /locus_tag="VN084_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWT28188.1"
                     /translation="MSLSKIERLSPAELKTNLLSGLTVALAMVPEAIAFALIAHVSPL
                     TGLYAAVIVSFITAAFGGRPGMISGATGALAVVMVALVVQHGVEYLFATVVVMGVLQA
                     LFAVAKLGKFIRMVPHPVMLGFVNGLAIVIFLAQFGHFKTVAADGSVTWMQGAALYTM
                     FGLIAATVTVIYLLPKLTKIIPSTLAAIMLVSLGVGLFGIDTKTVGDLGSIAGGLPSF
                     HLPQVPLNLETLRIVFPYALILAAIGLIETLLTLNLIDDMTDTRGKPNRESMAQGAAN
                     IVTGFFGGMGGCAMIGQSMINVNNGALKRLSGIATSAFLLSFILFAHRWIEMIPLAAL
                     IGVMFVVAAKTFEWGSFRLFGKVPKADIVVGLLVAGITVIADLAVAVIIGVIVSALVF
                     AWEHAKQINARSSINDQGQKVYELQGTLFFASIAAFQNLFSPQTDNDDVIIDFRDAKV
                     MDHSALAAIDALALRYQAAGKTLHLRHLSPDCLELLDQAQGMIEVNVLEDPRYHIADN
                     "
     gene            complement(1769..>2034)
                     /locus_tag="VN084_01655"
     CDS             complement(1769..>2034)
                     /locus_tag="VN084_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HWT28189.1"
                     /translation="ASLDITGREYFVTGVDDANRGGHDNILRADIAFTTRVVGNHGLS
                     IKYLWNQRDATYDALGDRSQITATLGLYYTYLGDDAFGAVDWR"
BASE COUNT          414 a          568 c          686 g          366 t
ORIGIN      
        1 gttgtcggcg atgtggtagc gggggtcttc cagcacattg acctcgatca tgccttgggc
       61 ctgatctagt agttccaggc agtctgggct caggtgccgc agatgcagcg tcttgccggc
      121 cgcctggtag cgaagagcca gcgcgtcgat tgctgccagc gcggagtggt ccatgacctt
      181 ggcgtcgcgg aagtcgatga tgacgtcatc gttatcggtt tgcggactga acaggttctg
      241 gaacgcagcg atcgaggcga agaacagcgt accttgcaat tcgtagacct tctgtccctg
      301 atcgttgatg gaagatcggg cattgatctg ctttgcgtgc tcccaggcga agaccagcgc
      361 ggagacgatg acgccgatga tgacggcgac ggcaagatcg gcgatgacgg tgatgccggc
      421 caccagcagg ccgaccacga tatcggcctt gggcaccttg ccgaacagcc tgaaactgcc
      481 ccattcgaag gtcttggcgg cgacgacgaa catgacgccg atcagcgccg ccagggggat
      541 catctcgatc cagcggtggg cgaacaggat gaaggacagc aggaaggcag aggtggcgat
      601 gcccgacagg cgcttcaagg cgccgttgtt cacgttgatc atgctttggc cgatcatggc
      661 gcaaccgccc atgccgccaa agaaaccggt gacgatattg gcggcgcctt gcgccatgga
      721 ttcgcggttc ggcttgccgc gcgtatcggt catgtcgtcg atcaggttca gggtcagcag
      781 agtttcgatc aggccgatcg cagccaggat cagggcatac ggaaagacga tacgcaaggt
      841 ctccaggttt aacggcactt gcggcagatg gaagctcggc aggccgccgg cgatggaacc
      901 gaggtcgccc acggtcttgg tgtcgatgcc gaacaggcct acacccagcg agaccagcat
      961 gatggctgcc agggtggacg ggatgatctt ggtcagcttg ggcagcaggt agatcaccgt
     1021 cacagtggcc gcgatgaggc cgaacatggt gtagagcgcc gcgccttgca tccaggtcac
     1081 gctgccgtcg gcggccacgg tcttgaaatg tccgaactgc gccaggaaga tgacgatggc
     1141 gaggccgttg acgaagccca gcatcacggg atgcggcacc atgcgaatga acttgccgag
     1201 cttggcgacc gcgaacagcg cctgcaagac gcccatgacc accacggtgg cgaacaggta
     1261 ctccacgcca tgctgcacca ccagcgccac catcaccacg gctagtgcgc cagtcgcgcc
     1321 ggagatcatg cccggtcgtc caccaaaggc ggcggtgatg aacgagacga tgacggcggc
     1381 atagagcccg gtcagcggcg atacatgggc gatcagtgca aaagcgatgg cttccggcac
     1441 catggccagc gcgacggtga ggccgctcaa cagattggtt ttgagctcag ccgggctgag
     1501 cctttcgatc ttggataagg acaagtgttt ctccaggcag gcgccgcata cgctgcgggc
     1561 cgcaagatga ttacgggatg gtcaggcgag ggtggggcag gcctgcgttc ggcaggctgg
     1621 ttgcggcatc acatgggtgg cggtatctat ggcgggcaga aaaccgctat tttagcagaa
     1681 atgggattta ggccaaaacc tcaacggata caaccacttg cgtcacgcgc gctggatgtt
     1741 gcgtggccag gcgttcagcg ccagtcgttc agcgccagtc gacggcgccg aaggcgtcat
     1801 cgccaaggta ggtgtaatag agacccagcg tggccgtgat ctgtgagcgg tcgcccaagg
     1861 catcataggt ggcgtcgcgc tggttccaca ggtacttgat gctgaggccg tggttgccga
     1921 cgacgcgtgt ggtgaaggcg atatcggcgc gcaggatatt gtcgtgcccg ccacggttgg
     1981 cgtcgtcgac gccggttacg aaatattcac ggccggtgat gtcgagcgag gccc
//
LOCUS       DATMTW010000086         1710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_74550, whole genome shotgun sequence.
ACCESSION   DATMTW010000086 DATMTW010000000
VERSION     DATMTW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74550"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VN084_01660"
     CDS             complement(<1..1182)
                     /locus_tag="VN084_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWT28190.1"
                     /translation="MTEAAQKFFMDPRRRMLAGFVLALSNFMVVLDLTIANVSVPHIA
                     GNLGISADQGTWIITSYAVAEAICLPLTGWLSQRFGTVRLFIGGMIGFGLFSFLCGIS
                     TTLGMIVACRIGQGICGAPLMPMSQTLLMRIYPPEQRGRVMGLWAMTTLAGPALGPII
                     GGYISDNWSWHWIFFINLPIAVICVFAAIALLTPAETERKKLPIDYIGLALLVFWIGC
                     LQIMLDTGRDHDWFADPTILMLAVLAGIGFLVFIIWELTEEHPIVDLRVFRHVGFTSG
                     VLTYSLCFGAYFAGIVIIPQWLQISMGYTAVWAGVVTAFTATTSIFVAPIAGRVVSKV
                     DVRILISASVTWMAVVTMWRAGWTSGADFWTLSMPQVVQGLAMPFFIIWELTEEHPIV
                     DL"
     gene            complement(1193..>1710)
                     /locus_tag="VN084_01665"
     CDS             complement(1193..>1710)
                     /locus_tag="VN084_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWT28191.1"
                     /translation="ELAANDALVSGSTIETDPAVMAAKAKLESARLDLGRTIIRAPID
                     GVVTRRQVQVGQRVAQGSPIMSIVPLAQVYVDANFKERQLRQVRVGMPATVVADIYGG
                     DVVYHGKVVGFSGGTGSSLALIPAQNATGNWIKVVQRLPVRIALDPRELAAHPLRVGL
                     STEVEIDLSRK"
BASE COUNT          369 a          548 c          520 g          273 t
ORIGIN      
        1 cagatccacg atcggatgct cttcggtcag ttcccaaatg atgaagaagg gcatggccag
       61 cccctgcacg acctgcggca tggacaaggt ccagaaatcg gccccgcttg tccatcccgc
      121 ccgccacatg gtgacgaccg ccatccatgt cactgacgca gagatcagga tgcgcacatc
      181 cactttgctc accacccgtc cggcgatcgg ggcgacgaag atggacgtcg tcgcggtaaa
      241 ggcggtcacc acgcccgccc agactgcggt gtagcccatg gatatctgca accattgcgg
      301 gatgatcacg atgcccgcga aataggcgcc gaagcagagc gagtaggtca gcacgcccga
      361 tgtgaaaccg acatggcgaa agacgcgcag atccacgatc ggatgctctt cggtcagttc
      421 ccaaatgatg aagaccagga agccgatgcc cgccagcacg gccagcatca ggatcgtggg
      481 atcagcgaac cagtcatggt cccggccagt gtccagcatg atctgaaggc aaccgatcca
      541 gaaaaccagc agggcaaggc cgatatagtc gatcggcagc ttcttgcgct cggtctcggc
      601 cggggtcagc aaggctatcg ccgcgaacac gcagatgacg gcgatcggca ggttgatgaa
      661 gaagatccag tgccagctcc aattgtcgct gatatagccg ccgatgatcg gccccagcgc
      721 gggaccggca agggtggtca tcgcccaaag ccccatcacc cttccgcgct gctcgggcgg
      781 atagatgcgc atgagcaagg tctgcgacat cggcatcagc ggcgcgccgc aaatgccctg
      841 cccgatacgg catgcgacga tcatgccgag cgtggtcgag ataccgcaca ggaaagagaa
      901 cagcccgaag ccgatcatgc cgccaatgaa cagccgcacc gtgccgaacc gctgggacaa
      961 ccagcctgta agcggcaggc agatcgcctc cgccacggcg taggaagtga tgatccaggt
     1021 tccctggtca gccgaaatgc ccaggttccc ggcgatatgc ggcaccgaca cattggcgat
     1081 cgtcaggtcg agtacgacca tgaagttgct caatgccagc acgaaccctg caagcatccg
     1141 ccgccggggg tccatgaaga atttttgggc cgcttcggtc acgcgccctg ccttatttgc
     1201 gcgaaaggtc gatctcgact tcggtcgaga ggcctacacg gagcggatgg gctgcgagtt
     1261 cacgtgggtc gagcgcgatg cgcacaggca ggcgctggac caccttgatc cagttgccgg
     1321 tagcgttctg cgccggaatc agcgccagcg acgacccggt gccgccggaa aagccgacga
     1381 ccttgccatg atagacgacg tccccgccat agatgtcggc gaccaccgtg gcgggcatgc
     1441 cgacgcgcac ctggcgcagt tgccgctcct tgaagttggc atcgacatag acttgggcaa
     1501 gcggcacgat gctcatgatc gggctgccct gcgcgacacg ctggccgacc tgcacctgac
     1561 gccgcgtcac cacaccatcg atgggcgcgc ggatgatcgt gcggccaagg tcaagccgcg
     1621 cactttccaa tttcgccttg gcggccatca cggccgggtc cgtctcgatg gtcgatccgc
     1681 tgacaagggc atcattcgcc gcaagctcac
//
LOCUS       DATMTW010000087         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_241872, whole genome shotgun sequence.
ACCESSION   DATMTW010000087 DATMTW010000000
VERSION     DATMTW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241872"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VN084_01670"
     CDS             <1..669
                     /locus_tag="VN084_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28192.1"
                     /translation="DKAVQVADPAFRLGVEPVELPSLFKGAADGVLAFNVSPLVADSW
                     KRRNPGGSLIDECAGFVRRVLAESSLGVALLPHVDPLDGSVENSDSAYMEGLLKACGG
                     TSDRLALMPRGLNASQIKSLVGASRFLIAARTHATVAGWSQFVPTLSIAYSIKAKGLN
                     QDLFDGLDYVLDTPKVSRSTLWDGLSLLRDREHDIRAHLAARIPVWRERAGLSAEVLA
                     KALG"
     gene            673..1692
                     /locus_tag="VN084_01675"
     CDS             673..1692
                     /locus_tag="VN084_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT28193.1"
                     /translation="MHRADCELTVIIKALNEARNIVRTAETARQATRGVDVQLLLADS
                     LSDDETVSLAQAQGLEVVQLLDRRDRSCGVGAQLGFQHARGRFILLIDGDMELQREWV
                     LAAMQRLSDDPSLAGVGGLIEDMNFDNIEFRARKQRNPVDAQPGLVDRLNGGGMFRRE
                     AIESVGYFTNRNLHACEELELGLRLTHAGWRMERLGLVAVRHYGHTDPLLKLVRKRWR
                     SHYVDGAGELLRASFGQPWSGKAWRGVSKLLVVLGWWALLLVLGSAVLAGVASGWTWL
                     VLAGTPPVLMMAKKRSVAMGAYAVFAWCVDLAGLLRGILRRQTDPRQPVSCRVLPSPA
                     APGAV"
     gene            1698..2279
                     /locus_tag="VN084_01680"
     CDS             1698..2279
                     /locus_tag="VN084_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28194.1"
                     /translation="MPSDPKPTLGDCIRADVWRCQGAFSWPLVLRSWWQDPAVRPVLT
                     LRFVQACDAGRGPAWRLLSACLRIWHRRTQARCGVDLPRSLQVGPGFKLLHGWGIVIN
                     RDARIGRNVTIMQGVTIGGTQSGIPVIEDDVIVCANATVVGRLTLGQGAVVGAGCVVT
                     RDVAPHTTVVGNPQRVIERRGPIKPYNPAPLQH"
     gene            2290..>3714
                     /locus_tag="VN084_01685"
     CDS             2290..>3714
                     /locus_tag="VN084_01685"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWT28195.1"
                     /translation="MKFDLLLKHVCALSACTFAGFVLCAVAHADDGGWRGVEDRSLEV
                     QGANNPLDFSSLLPVHAAAGSNGWAIATPDGHIAFEHQSSPTRFLCASFVFSALNGGL
                     PSKEDAPRLVQALRRQAYNLVRLHFVDATLMAGRKKDFDFDPDQLDRLFFLLAELKKA
                     GIYWLVDGMTSDNAAYGDVEPNRYVKKHHAKMEVLTTEAGWQHWATLVEKLWGRKNPY
                     TGLPPLKDPAMLGMILVNEGSLAYQASIEGGHYSDTLGPGFTSWLRQRYNDDSGLARA
                     WQREKQDSESMQGSVSLPSEVRARGARGADFSRYIVDLERRGVARMEKHVRDLGFKGL
                     TTAFDNWGFLQADISRAASGWVDMHSYASLPSQHGQAGSVLEQTSVHNNVARYVRELS
                     NARQWGKPFTVSEWGHPFWNQWRYESALLMPAMAGLQGWDAICQFAETPIQFEYGTVG
                     PKRLQAMYPFGVGGDPIMRAAERLA"
BASE COUNT          639 a         1134 c         1243 g          698 t
ORIGIN      
        1 gacaaggcgg tccaggtggc cgaccccgcc ttccgcctag gtgtggagcc ggtggaattg
       61 ccgtcgctgt tcaagggtgc ggccgacggc gtgctggcgt tcaatgtgag ccccctggtg
      121 gccgacagct ggaagcgccg caatcctggc ggctcgctga tcgatgaatg cgctggcttc
      181 gtacgtcgcg tattggccga atcctcgctg ggcgtggcgc tgctgcctca tgtcgacccg
      241 cttgatggca gcgtggagaa cagcgactca gcctacatgg aaggcttgct gaaggcctgc
      301 ggaggcactt cggaccgcct ggcactgatg ccgcgcgggc tcaatgcgtc ccagatcaag
      361 tcactcgtgg gtgccagccg cttcctgatc gcggcgcgca cccatgcgac cgtggctggc
      421 tggtcccagt tcgtgcccac gctgtctatc gcctacagca tcaaggccaa gggtttgaat
      481 caggacctgt tcgatggcct ggattacgtg cttgacacgc ccaaggtatc gcggagcacg
      541 ctgtgggatg gcctgtcgct tctgcgtgat cgcgaacatg acatccgcgc gcatttggct
      601 gcacgcattc ccgtgtggcg cgagcgagca gggctcagcg ctgaggtcct ggcgaaagct
      661 cttgggtaga ccatgcatcg cgccgactgc gaactcacgg tcatcatcaa agcgctgaac
      721 gaggcgcgca atatcgtgcg aacggccgag acggcgcgcc aggctacacg cggcgttgac
      781 gtacaactgc tgctcgccga ctcgctgtcc gacgacgaga cggtcagtct cgcccaggcg
      841 cagggccttg aggtggtgca actgctcgac cggcgcgacc gctcctgcgg cgtgggcgcc
      901 cagctcggct tccagcatgc gcgcggcagg ttcatcctgc tcatcgacgg cgacatggaa
      961 ctgcagcgcg agtgggtgct agctgccatg cagcggctgt ccgatgaccc aagcttggcc
     1021 ggcgtgggtg gactgatcga ggacatgaat ttcgacaaca ttgagttccg agcaagaaag
     1081 cagcgcaatc cggttgacgc gcagcccggc ctcgtggacc gactcaatgg tggaggcatg
     1141 ttccggcgcg aggcgatcga gtcggttggc tacttcacaa atcgcaacct tcacgcgtgc
     1201 gaggagctcg aactcgggct tcggctgacg cacgcgggct ggcgcatgga gcgcctgggc
     1261 ctggtggcgg tgcgccacta tgggcacacg gacccgctgc tgaagctggt tcgcaagcgc
     1321 tggcgctccc actatgtcga tggcgctggc gagctgctgc gcgccagctt cggtcagccc
     1381 tggagcggca aggcatggcg cggcgtctcc aaactgttag tggtactggg atggtgggcc
     1441 ttgctgctcg tgctcggctc ggccgtgttg gccggtgtcg ccagcggttg gacctggctg
     1501 gtattggccg gcaccccgcc tgtgctcatg atggccaaga agcgcagcgt ggctatgggt
     1561 gcctacgccg tgtttgcctg gtgtgtggac ttggctgggc tgctgcgcgg cattctccgc
     1621 aggcagaccg atccccgcca gcccgtgtcc tgccgggtgt tgccgtcccc cgctgcgccc
     1681 ggcgcggtct gacgagcatg ccgtccgatc ccaaaccaac cttgggtgac tgcatccgcg
     1741 ccgacgtctg gcgatgccag ggcgcgttca gctggccgct ggtgcttcga agctggtggc
     1801 aggacccagc ggtcaggccc gtgttgacgt tacgcttcgt tcaggcctgc gatgcgggcc
     1861 gaggcccggc atggcgcctg ttgagcgcgt gtctccgcat ctggcatcga aggacgcagg
     1921 cgcgttgcgg tgttgaccta ccgcgcagcc ttcaggtcgg tccaggtttc aaactgttgc
     1981 atggctgggg cattgtgatc aatcgcgatg cccgcattgg ccgaaacgtc acgatcatgc
     2041 aaggcgtcac catcggtgga acgcagagcg gcattcctgt aatcgaggat gacgtcatcg
     2101 tgtgcgcaaa cgccacagta gttggcagac tcacgctggg ccagggcgcc gtcgtcggcg
     2161 ccgggtgcgt cgtcactcga gatgtcgcac cgcacaccac ggtggtgggt aacccgcagc
     2221 gggtgatcga aaggcgaggg ccgatcaagc cctacaaccc ggcaccgctg cagcattagc
     2281 gagctggcca tgaagtttga tctcctcctc aagcacgttt gcgcgctttc ggcgtgcacc
     2341 tttgcagggt ttgtcctttg cgccgttgcc catgccgacg atggtggctg gcgtggcgtg
     2401 gaggaccgca gcctcgaagt gcagggcgcg aacaacccgc tggatttcag cagcctgctg
     2461 cccgtccacg ccgcggcggg gagcaatggc tgggccattg cgacgcctga cgggcacatc
     2521 gccttcgagc accagtccag cccgacgcgc ttcctgtgcg cgtcctttgt attcagcgcg
     2581 ctcaatggcg ggctgccaag taaggaggac gctccgcgct tagtacaagc actgcgtcgg
     2641 caagcctata acctggttcg gctgcatttc gtcgacgcta ctttgatggc aggacgcaag
     2701 aaggatttcg acttcgatcc tgatcaactc gaccggctgt tcttcctgct cgccgagctg
     2761 aagaaggcgg gcatttactg gttggtcgat ggcatgacct ccgacaacgc tgcgtatggg
     2821 gacgtggagc ccaatcggta cgtcaaaaag catcacgcaa agatggaggt gctcaccacc
     2881 gaggcgggct ggcagcactg ggcgactctg gtcgagaaac tgtgggggcg caaaaatccc
     2941 tacactggcc taccgccgtt gaaggaccct gccatgctcg gcatgatcct cgtcaatgag
     3001 gggtcgcttg cctaccaggc cagcatcgaa ggcgggcatt actcggacac gttggggcca
     3061 gggttcacca gctggcttcg ccagcgctac aacgatgaca gcgggctcgc tcgcgcttgg
     3121 cagcgagaaa agcaggatag cgagtctatg caaggcagcg tgtccttgcc gagcgaagtg
     3181 cgcgcgcgcg gcgcgcgcgg cgcggatttc tcgcgctaca tcgtcgacct ggagcgacgc
     3241 ggcgtggcgc gaatggaaaa gcatgtgcgc gatctcggct tcaaggggct cacgactgca
     3301 ttcgacaact ggggcttcct gcaggccgac ataagtcgcg ctgccagcgg ctgggtggat
     3361 atgcacagct atgcaagcct gccttcgcaa catggccagg cggggtcagt gctcgagcag
     3421 accagcgtgc acaacaacgt ggcgcggtac gtgcgcgagt tgagtaacgc gcgacaatgg
     3481 ggaaagccct tcaccgtgag cgaatggggc catcccttct ggaaccaatg gcgctacgag
     3541 tctgcactgc taatgccggc catggcggga ctccagggat gggacgcaat ctgccagttc
     3601 gcggagacgc cgattcagtt tgaatatggc actgttggcc cgaagcgcct tcaagccatg
     3661 tacccgttcg gcgttggtgg cgacccaatc atgcgtgcgg ccgagcgact cgcc
//
LOCUS       DATMTW010000088         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_175168, whole genome shotgun sequence.
ACCESSION   DATMTW010000088 DATMTW010000000
VERSION     DATMTW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175168"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VN084_01690"
     CDS             complement(<1..355)
                     /locus_tag="VN084_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28196.1"
                     /translation="MPSKATAPWRSYLSRRFCLALLLSLLLHLFVVHGFGFLKLPVDG
                     TEPTMTEARLVPLPPKPPAKIAARPAKPPKPVKRPQPQPAEPVLEPLPPRLPIPEPPA
                     PSIAEPVAADAPAQPA"
     gene            complement(345..1040)
                     /locus_tag="VN084_01695"
     CDS             complement(345..1040)
                     /locus_tag="VN084_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWT28197.1"
                     /translation="MKHMISAGFGMMLALVSLAVSAAPKQVDLVYEATRNGQPFATVT
                     ESYRQENGRYRIESVTKGVGVYALFGERRLISEGEVTSDGLKPTHFELHQGDNARKSL
                     FADFDWATHTLTMKVKGSPRTAPLEEGAQDLSSMLYQFMFKQPAGELFSLPVTTGKKL
                     RNYEYKVSARDLPLEVPAGTYKTIHLVEAEATGDEKELWLGAESHYLPVKLLLHDDNG
                     SKIEQTLTSLHAE"
     gene            complement(1121..>1753)
                     /gene="purM"
                     /locus_tag="VN084_01700"
     CDS             complement(1121..>1753)
                     /gene="purM"
                     /locus_tag="VN084_01700"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWT28198.1"
                     /translation="ALVGGETAEMPGMYPAGEYDLAGFAVGAVDRSAIINGSTIAEGD
                     VVLGLASSGAHSNGYSLIRKLIDQSGIDMDADFHGKPFRDVVMAPTRIYVKPLLKLLA
                     AIPVKGMAHITGGGITENIPRVLPAGLTAEIRAGSWAMPPLFSWLQAQGNVTDDEMYR
                     TFNCGIGMAVIVSQQNAEAARALLAAEGEQVWAIGYIRRQQVEEAPTVVV"
BASE COUNT          311 a          532 c          562 g          348 t
ORIGIN      
        1 gtgccggttg cgcgggcgca tctgccgcaa ccggctcggc aatcgagggt gccggcggct
       61 ccggaatggg cagtcgcggc ggcaagggtt cgagcacagg ttcagccggc tggggctggg
      121 gccgcttgac tggtttgggc ggtttcgcag gccgggcagc gatcttggct ggcggcttgg
      181 gcggcaaggg taccagccgt gcctcggtca tcgtgggttc ggtaccatcg accggcaatt
      241 tgaggaaacc gaatccatgg accacaaata gatgcaacag cagggaaagc aacagcgcca
      301 ggcaaaaacg ccgggaaaga tagcttcgcc atggcgcagt ggctttactc ggcatgcagg
      361 ctggtcaacg tctgctcgat tttgctaccg ttatcgtcat gcagcagcag tttcaccggc
      421 aggtaatggc tttcggcacc cagccacaac tccttctcat cgccggtcgc ttcggcttcc
      481 acaagatgga tggtcttgta agtgcctgct gggacttcca gcggcagatc gcgggcgctg
      541 accttgtact cgtaattgcg cagcttcttg ccggtggtca ccggtaggct gaacagttcg
      601 cccgcaggct gcttgaacat gaactggtag agcatgctgc tgaggtcctg cgcgccttct
      661 tccagcggcg ccgtgcgcgg gctgcccttg accttcatgg tcagcgtatg ggtcgcccag
      721 tcgaaatcgg cgaagagcga tttgcgcgca ttgtcgccct gatgcagttc gaaatgggtg
      781 ggtttgaggc cgtcactcgt gacttcgccc tcactgatca agcggcgttc gccgaacaag
      841 gcatagacgc caacgccctt ggtgacgctc tcgatacggt agcgcccatt ctcctggcgg
      901 tagctttcgg tcacggtcgc gaaaggctgg ccgttacggg tggcctcata gaccaggtcg
      961 acttgcttgg gagcggcgct gaccgccaga ctgaccaggg ccagcatcat gccgaaccct
     1021 gctgagatca tgtgcttcat cttgcctcct attcggtgcg tcacgtctgt catctaacgc
     1081 gatggcaact gattggttgg caatgcgcag gcggacaagt tcagacgacg acggtcggcg
     1141 cttcttcgac ctgctggcga cggatgtacc caatggccca gacctgctca ccttcggccg
     1201 ccagcaaggc acgcgcagct tcggcattct gctggctgac gatgaccgcc atgccgatgc
     1261 cacagttgaa ggtacggtac atctcatcgt cggtgacgtt gccctgcgcc tgcagccagc
     1321 tgaacagcgg gggcatggcc cagctccccg cccggatctc ggccgtgagg cccgccggca
     1381 acacgcgcgg gatgttctcg gtgatgccgc cgccagtgat gtgcgccatg cccttgaccg
     1441 gtatcgccgc cagcagcttg agcagcggct tgacatagat gcgcgtcggc gccatgacga
     1501 catcgcggaa tggcttgccg tggaagtcag cgtccatatc gataccggat tggtcgatga
     1561 gcttgcggat cagcgaataa ccgttggagt gcgcgccgct ggaagcgagg cccagcacca
     1621 cgtcaccttc ggcgatggtg ctgccgttga tgatggcgct gcgatcgacg gcaccgaccg
     1681 caaaaccggc gagatcgtat tcgccggccg gatacatgcc cggcatctcg gcggtttcac
     1741 cgcccacgag cgc
//
LOCUS       DATMTW010000089         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_309101, whole genome shotgun sequence.
ACCESSION   DATMTW010000089 DATMTW010000000
VERSION     DATMTW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309101"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="glnE"
                     /locus_tag="VN084_01705"
     CDS             complement(<1..1334)
                     /gene="glnE"
                     /locus_tag="VN084_01705"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HWT28199.1"
                     /translation="MPNLDQILQQNATLTDDEKRLAHAVWCSPYLKRLLESDATLLPE
                     LMAQLHQPWTPADMQDWLAAQPVNDEATLKRALRKLRQRVMLRLIVRDLGGLGDLDEV
                     MRVTSALAEVAVQFALQRLTPDLQRQYGAPIGESGKPQALLVVGMGKLGGGELNVSSD
                     IDLIFAFDEDGETDGERPISNGEFFTRLGKKLIAAIDDVTEDGFVFRVDMRLRPYGSE
                     GPLACSLAMLEDYYQNQGREWERYAWIKGRVIAGEGKTLADLLRPFVFRKYLDYGAFA
                     SMRDLKVQIQRDVNRRDLHDNIKLGRGGIREIEFIAQVFQLIRGGRDASLQIRPTLAV
                     LQLLGEKGLMPQATVDELTVAYVFLRNLEHRLQYLEDAQTQDLPKNEAAQARITRAMG
                     FDDWAAFREALEIHRQRVQRHFTELFSEPEGKDTPDEDGPLKQAKALWQGLLA"
     gene            1430..3394
                     /gene="acs"
                     /locus_tag="VN084_01710"
     CDS             1430..3394
                     /gene="acs"
                     /locus_tag="VN084_01710"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWT28200.1"
                     /translation="MSSIESVLSETRVFPPHESFKQSATVSGMSAYQALCDQATQDYT
                     GFWADLARQELDWHKPFSQVLDESNAPFYRWFHDGQLNASYNCLDRHLASRGDKTALI
                     FEGDDGQTTNITYRQLHQRVCRFANVLKSQGIGLGDRVIIYLPMSIEAVVAMQACARI
                     GAIHSVVFGGFSAKSLHERITDVGASLIITADGQFRGGKAMPLKPAVDESLTYGGCEG
                     VKKVIVYKRTGGEVQWDANRDVWWHELESTASDQCEPTWVSAEHPLFILYTSGSTGKP
                     KGVQHSTGGYLLGAIMSMKWVFDYKSADIFWCTADVGWITGHTYVTYGPLALGGTEVI
                     FEGVPTYPDAGRFWQMIEKHKVSIFYTAPTAIRSLIKLGADLPNQYDLSSLRLLGTVG
                     EPINPEAWMWYHTVVGKERCPIADTWWQTETGAHMIAPLPGAVPTKPGSCTLPLPGIM
                     MDIVHEDGTPVEGTGGGLLVVKKPWPSMIRTIWNDPERFKKSYFTEELKGCYLAGDSS
                     HRDADGYFWIMGRIDDVLNVSGHRLGTMEIESALVANPLVAEAAVVGKPHDIKGEAVV
                     AYVVLKGARPEGDAAKKIVAELRDWVGKEIGPIAKPDEIRFGENLPKTRSGKIMRRLL
                     RSLAKGEEITSDISTLDNPAILEQLKQAVS"
     gene            complement(3465..>3804)
                     /locus_tag="VN084_01715"
     CDS             complement(3465..>3804)
                     /locus_tag="VN084_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWT28201.1"
                     /translation="VYGVGFYQPENDHGDGGLGNPWLYFDGKDGKPIGAKIPGAGSAG
                     DIFMQAQFPLHSGRILGLPGRIMISIMGLVVAMLSLTGIVIWLHKQRNRALQRYRQVK
                     SGPSFIGRGV"
BASE COUNT          762 a         1227 c         1079 g          736 t
ORIGIN      
        1 gctagcaagc cctgccacag cgctttcgcc tgcttgagtg gcccgtcttc atctggagta
       61 tccttgcctt caggttcgct gaaaagttcg gtgaaatgtc gctggacccg ctgacgatgg
      121 atttccagcg cctcacggaa ggccgcccag tcgtcaaagc ccatggcgcg cgtgatccgt
      181 gcctgcgccg cctcgttttt cggcagatcc tgggtctgcg catcttcaag atattgcagg
      241 cggtgctcca gattccgcag gaatacatag gccacggtca attcatccac cgtggcttgc
      301 ggcatcagcc ctttttcacc gagcaactgc aagaccgcca gcgtcggccg gatctgtagg
      361 ctggcgtcac ggccgccgcg aatcagctgg aatacctgcg cgatgaactc gatctcgcga
      421 atgccaccgc gcccgagctt gatgttgtcg tgcaagtcac ggcgattgac gtcgcgctgg
      481 atctgcacct tgagatcgcg catggaagcg aacgccccat aatccaggta tttgcggaag
      541 acgaacggcc gcagcaggtc ggccagcgtc ttgccttcgc cagcgatcac ccgccccttg
      601 atccaggcgt aacgctccca ctcgcgcccc tgattctggt aatagtcttc gagcatggca
      661 aggctgcaag ccagcggccc ttccgagcca tacgggcgca gtcgcatgtc gacgcggaag
      721 acgaacccat cctcggtgac gtcgtcgatg gcggcgatca gtttcttgcc cagccttgta
      781 aagaactcgc cattgctgat cgggcgctcg ccatcggttt cgccatcctc gtcgaaggcg
      841 aagatgaggt cgatatcgga cgagacgtta agctccccgc cgccgagctt gcccataccg
      901 accaccagca acgcctgcgg cttgcctgat tctccgatgg gtgcgccata ttgccgctgc
      961 aggtccggcg tcaagcgttg cagggcgaac tgcacggcga cttcagccaa ggcagaggtc
     1021 acccgcatga cctcgtcgag gtctcccagc ccacccagat cgcgcacgat cagtcgcagc
     1081 atgacgcgct ggcgcaattt acgcagcgca cgcttgagcg tggcctcatc gttcaccggt
     1141 tgcgccgcca gccaatcctg catgtcggca ggtgtccagg gctggtgcaa ttgcgccatc
     1201 agttcaggca gtaaggtcgc atcgctttcg agcaggcgtt taaggtaggg actgcaccag
     1261 acggcgtgcg cgaggcgctt ttcgtcatcg gtcagggtgg cgttctgctg caatatctgg
     1321 tcgaggtttg gcatctcaat atcggatgat tgggttatca tgcttattct aagcggtttg
     1381 ccataaatca taagaaatca gttgcataag tattctctgg aggagtttta tgtcgtcgat
     1441 cgaatccgtg ctttcagaaa cccgtgtctt ccccccgcac gaatcattca agcaatctgc
     1501 caccgtttcc ggcatgtcgg cttatcaggc gctatgcgat caagccactc aggattacac
     1561 cggtttctgg gctgacctgg cacggcagga actcgactgg cacaaaccct tcagccaggt
     1621 cctcgacgaa tccaacgcgc ctttctatcg ctggttccat gacggccagc tgaatgcctc
     1681 ctacaattgc ctcgaccgcc acctggcctc gcgcggcgac aagactgcgc tgatcttcga
     1741 aggcgacgac ggccaaacca ccaacatcac ttacaggcag ctccatcagc gcgtctgccg
     1801 attcgcgaac gtattgaaat cgcaaggcat cggccttggc gaccgcgtca tcatctacct
     1861 gccgatgagc atcgaggcag tcgtcgccat gcaagcctgt gctcgcatcg gcgccatcca
     1921 ctccgtggtg ttcggcggct tctccgccaa gagcctgcat gaacgcatca ccgatgtcgg
     1981 tgcctcgctc atcatcacgg ctgacggtca gttccgcggc ggcaaggcca tgccgctcaa
     2041 acctgcggtc gacgaatcgt tgacctacgg cggttgcgaa ggcgtgaaga aagtcatcgt
     2101 ctacaagcgc accggcggcg aggtgcaatg ggacgcgaat cgcgacgtct ggtggcatga
     2161 actcgaaagc acggcgtccg accagtgcga gccgacctgg gtcagcgcag agcatccgct
     2221 gttcatcctc tacacctccg gttcgaccgg caagcccaag ggcgtgcaac actccaccgg
     2281 cggctatctg ctcggcgcca tcatgagcat gaaatgggtg ttcgactaca aatccgccga
     2341 tatcttctgg tgcacggccg acgtcggctg gatcaccggc cacacctacg tcacctatgg
     2401 cccgctggcg ctgggcggca ccgaagtcat cttcgagggc gtgcccacct atccggatgc
     2461 cgggcgtttc tggcagatga tcgaaaagca caaggtcagc atcttctaca ccgcgccgac
     2521 cgccatccgc tcgctgatca agctcggtgc cgacctgcct aaccaatacg atctctccag
     2581 cctgcgcctg ctgggcaccg tgggcgaacc gatcaacccg gaagcctgga tgtggtacca
     2641 caccgtggtc ggcaaggaac gctgtcccat cgccgatacc tggtggcaga ccgagaccgg
     2701 cgcgcacatg attgcgccgt tacccggcgc cgtaccgacc aagcccggct cttgcaccct
     2761 gccgctaccc ggcatcatga tggatatcgt gcatgaggac ggcacacccg tcgaaggcac
     2821 cggcggcggc ctgttggtcg tgaagaagcc ctggccttcc atgatccgca ccatctggaa
     2881 cgacccggag cggttcaaga aatcctactt cacggaagaa ctcaaaggct gttacctcgc
     2941 gggcgattcc tcgcaccggg acgccgatgg ctacttctgg atcatgggcc gtatcgacga
     3001 cgtactgaac gtctcgggcc accgtctcgg caccatggag atcgaatcgg cgctggtcgc
     3061 caacccgctg gtggcagaag ccgccgtcgt cggcaagcca cacgatatca agggcgaagc
     3121 cgtagtggct tacgtggtgc tgaagggtgc acgccccgaa ggcgatgccg cgaagaagat
     3181 cgtcgccgaa ctgcgcgact gggtcggcaa ggagatcggc cctatcgcca agccggacga
     3241 gatccgcttc ggcgagaacc tgcccaagac ccgctccggc aagatcatgc gccgcctgtt
     3301 gcgctcactg gccaagggcg aggagatcac ttccgatatc tccacgcttg ataacccggc
     3361 gatcctggaa cagctgaaac aggccgtctc ctgaatctga cggtcgcaat agaaaaaacc
     3421 ggccagtggc cggttttttc tttttcacac ccccctgccg atgttcacac ccccctgccg
     3481 atgaacgatg gccctgactt gacctgccgg taacgctgca aggcgcggtt gcgttgcttg
     3541 tgcaaccaga tgacgatacc ggtaagcgac agcatcgcca ccaccagccc catgatggag
     3601 atcatgatac ggcccggtaa gccaaggatg cggcccgaat gcagcgggaa ctgcgcctgc
     3661 atgaagatat cgccagcgct gcccgcaccg ggaatcttgg cgccgatcgg cttaccgtcc
     3721 ttgccatcga aataaagcca gggattacct aggccaccat cgccgtggtc attctccggt
     3781 tgatagaacc ccacgccgta gacc
//
LOCUS       DATMTW010000090         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_443430, whole genome shotgun sequence.
ACCESSION   DATMTW010000090 DATMTW010000000
VERSION     DATMTW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443430"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VN084_01720"
     CDS             <1..1098
                     /locus_tag="VN084_01720"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWT28202.1"
                     /translation="REVDTVARLGGDEFIVLLENLHESAEIAASTSESVAEKILHQLR
                     QDFTLEGYVYHSSASIGISLFSQQEVNAEELLKRADTAMYQAKNAGRNTLRFYDPAMQ
                     HALESRAALETDLRHALEDNQFQLYFQCQAYHNRQIISAEILLRWIHPQRGMISPLEF
                     IPLAEETGLIVPIGQWVLESACAQLKAWEHDVETAFLKLSINVSARQFHQDDFVEQVT
                     QTVLKSGIDPDKLDIELTESLVLDDINETIRKMEALREIGVRFSMDDFGTGYSSLAYL
                     TQLPLDKLKIDQSFVRNIGVKDSDAVIVQTIIGMASNLRMEVIAEGVETEAQRAFLEA
                     HGCPVCQGYLFGKPVPYAEFMRQLRDQAGPA"
     gene            complement(1111..1959)
                     /locus_tag="VN084_01725"
     CDS             complement(1111..1959)
                     /locus_tag="VN084_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWT28203.1"
                     /translation="MTLRVLIAGCGDLGLGVVSALAHTRFDIHGLRRSAQPCPNGVTA
                     VQADVTRPDSLGALRTLRPDVLLYCVAADAQTDDSYRMHYVDGLRNVLATLDPAQLRH
                     VFFVSSTRVYGQRADVLLDESTPAVPADFGGERLLQAEGLLQGLPCGTTVLRLSGIYG
                     PGRTRMLRLAAEPERWPAENVWSNRIHRDDAAAFTAFLIERVAAGMPVEHLYLVTDDL
                     PVPQQEVLRWIAGQLGVDCTQTPVMPVSGGKRLSNQRLRATGFELAYPDYRAGYGEML
                     RSGHEA"
     gene            complement(1956..>2187)
                     /locus_tag="VN084_01730"
     CDS             complement(1956..>2187)
                     /locus_tag="VN084_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWT28204.1"
                     /translation="QHPCIGYSNEVIHIYLARDLSAGEHRRDEDEHLQLFDASLPDCL
                     RMVRDGRITDGKTIIALFWAEKVLQGAWPLDT"
BASE COUNT          477 a          671 c          667 g          372 t
ORIGIN      
        1 cgcgaggtcg ataccgtggc ccggctcggt ggcgacgaat tcatcgtgct gctggagaac
       61 ctgcacgaga gcgccgagat cgctgccagc acgagtgaga gtgtggctga gaagatcctg
      121 catcagctcc gccaggattt cacgctggaa ggctatgtct accatagctc cgccagcatc
      181 ggcatcagcc tgttcagcca acaggaagtg aatgcggaag aattgctgaa acgcgccgat
      241 accgcgatgt accaggccaa gaacgcgggg cgcaacaccc tgcgcttcta cgacccggcg
      301 atgcagcatg cgctcgaaag ccgcgcagcg ctggagacgg acttgcggca tgcgctggag
      361 gataaccagt tccagctcta tttccagtgt caggcctatc ataaccggca gatcatcagc
      421 gccgagatcc tgttgcggtg gatacacccg cagcgcggca tgatctcccc gctcgaattc
      481 atcccgctcg ccgaagagac cgggctcatc gtaccgatcg gccaatgggt gctcgagagc
      541 gcctgtgcac aactcaaggc ctgggagcat gatgtggaga ccgcgttcct caagctctcc
      601 atcaacgtca gtgcgagaca gttccatcag gacgatttcg tcgagcaggt cacccagacc
      661 gtactcaaga gtggcatcga ccccgataag ctggatatcg agctgaccga gagtctggtg
      721 ctggacgaca tcaacgagac catccgcaag atggaagcct tgcgcgagat cggggtgcgc
      781 ttctccatgg acgacttcgg aacgggctat tcctcactgg cctacctgac ccagctgccg
      841 ctggacaagc tcaagatcga ccaatccttc gtgcgtaata tcggcgtcaa ggacagcgat
      901 gccgtgatcg tgcaaaccat catcggcatg gccagcaacc tcaggatgga agtgatcgcg
      961 gaaggcgtcg agaccgaagc gcaacgcgcc ttcctcgaag cgcacggatg cccggtatgt
     1021 cagggctacc tgttcggcaa gccagtgcct tatgcggaat tcatgcgaca gctacgagac
     1081 caggcaggac cagcgtgagc gcagatggct tcaagcctcg tggccggatc ggagcatctc
     1141 tccatagccc gcacgatagt ccgggtatgc cagctcaaag ccggtagcgc gtaaccgctg
     1201 gttactgagc cgcttgccgc cggacacagg catgactggc gtttgcgtgc aatcgacgcc
     1261 caactgcccg gcgatccagc gcagcacctc ttgctgcggt accggaaggt catccgtcac
     1321 cagatacaag tgctcgaccg gcatacccgc cgccacgcgc tcgatgagga atgcggtgaa
     1381 agccgcggca tcgtcgcggt ggatgcgatt gctccagaca ttctcggctg gccagcgctc
     1441 tggttcagcc gccagccgca acatacgcgt acgcccgggc ccgtagatgc ccgacaggcg
     1501 cagcaccgtt gtgccgcaag gcagcccctg caacaagccc tctgcctgaa gcagccgttc
     1561 accgccgaaa tcagcgggta cagcgggggt cgactcgtca agcaagacat cggcgcgctg
     1621 accatagacg cgcgtactgg aaacgaaaaa gacatgcctg agctgggcgg ggtcgagtgt
     1681 agccaggacg ttgcgcaggc catcgacata atgcatgcgg tagctgtcgt cggtctgcgc
     1741 gtcggcggcg acgcaataga gcaggacgtc aggacgcagt gttcgcaacg cgccaagaga
     1801 gtctggacgc gtgacatcgg cttgcacagc cgtcacacca ttggggcaag gctgcgcgct
     1861 gcggcggagg ccatggatat cgaatcgggt atgagcgagc gccgatacga cccccaggcc
     1921 gagatcgccg caaccagcga tcagtacgcg caaggtcatg tatccagggg ccaggcgccc
     1981 tgcaatacct tctcggccca gaacagcgcg atgatggtct tgccatcggt gatcctgcca
     2041 tcacgcacca tgcgcagaca atccggcaag ctggcatcga atagctgcag atgctcatcc
     2101 tcatcgcgtc ggtgctcgcc tgcactgagg tcgcgagcaa ggtagatatg gatgacttcg
     2161 ttggagtaac cgatgcaggg atgctgc
//
LOCUS       DATMTW010000091         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_175492, whole genome shotgun sequence.
ACCESSION   DATMTW010000091 DATMTW010000000
VERSION     DATMTW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175492"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VN084_01735"
     CDS             <1..377
                     /locus_tag="VN084_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWT28205.1"
                     /translation="KIVDLIRRQVREQPVEGLGEDLPDEPGMDEFFEQDGRHWDDKPL
                     AWDMPDNALEQKQFLGVLQECMDRLPAKLASLFMLREVQEEDNEKICKELDITPTNAW
                     VMLYRARMGLRKCLELNWLHRS"
     gene            414..665
                     /locus_tag="VN084_01740"
     CDS             414..665
                     /locus_tag="VN084_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWT28206.1"
                     /translation="MLSCKQASQLLSQSLDRRLSWRERMGLRLHLTVCDVCRRFGRQI
                     LVMRQAIRSLVRAVEQDEQISIPIDVKGRIAKAIDSKRG"
     gene            741..1076
                     /locus_tag="VN084_01745"
     CDS             741..1076
                     /locus_tag="VN084_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28207.1"
                     /translation="MKASKKTLAVVVGSAFVASIAATSVNAATNPFQAKTLTAGYMLA
                     DGSDKMKDGKCGTGKCGAEKKKMMEEKAKEGSCSADKAKEGSCHADKAKEGSCSADKA
                     KEGSCHADK"
     gene            1084..1959
                     /locus_tag="VN084_01750"
     CDS             1084..1959
                     /locus_tag="VN084_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HWT28208.1"
                     /translation="MAQTEKIHGTGLGLKRELIPQIQSAWAEGGRHNPLVANIDFLEI
                     APENWIGAGGRTAKQLAWFTERFPMVCHGLCLSLGGPDPLNEPFLQRVKQFLTQHHIP
                     RYTEHLSYCSDGGYLYDLLPIPFTEEAVHHVAGRIRRTQDILERRIAVENASFYAAAP
                     ISEMDEPSFIRAVLEEADCDLHLDLNNIYVNSVNFGFDPHAFLRSIPGERIVYAHVAG
                     HDREADDLAIDTHGQDVIDPVWALLREAYDLYGVFPTLLERDTNIPPLERLVREIDYI
                     AELQAPHRQSARAVL"
     gene            1989..>2162
                     /locus_tag="VN084_01755"
     CDS             1989..>2162
                     /locus_tag="VN084_01755"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HWT28209.1"
                     /translation="MPAFQRYQLSFTGHIRDPQHQPKPDKVAARRMRVYTEIVFNNLY
                     GAVSACFPVAQKVL"
BASE COUNT          486 a          613 c          642 g          421 t
ORIGIN      
        1 acaagatcgt cgacctcatc cgccggcagg tccgcgaaca gccggtcgaa gggctgggtg
       61 aagacctgcc ggacgagccg ggcatggacg aattcttcga gcaggacggc cggcattggg
      121 acgacaagcc gctggcctgg gacatgccgg acaacgcgct ggagcagaag cagttcctag
      181 gcgtcttgca ggagtgcatg gaccgcctgc cggcgaaact cgccagcctg ttcatgttgc
      241 gcgaggtgca ggaagaagat aacgaaaaaa tatgtaagga actggatatc acgccgacga
      301 atgcatgggt gatgctgtat cgcgcccgaa tgggattacg taaatgtctg gagttgaact
      361 ggttgcatcg gtcatgacct gtcgatgtga ctgttgcgca ggatgaacga cgaatgttga
      421 gttgcaaaca agctagccaa ttgctgtctc agtcgctcga ccgtcgatta tcctggcggg
      481 agcgcatggg tctgcgcctg cacttgacgg tgtgtgatgt ctgccggcgg ttcggtagac
      541 agatcctcgt catgcggcag gcgatcagga gcctggtgcg cgccgtggag caagacgaac
      601 agatcagtat tcccattgat gtcaaagggc gcatcgccaa ggcgatcgat tcgaaacgcg
      661 gttaaccggg tttagatacc gaactcgctg tgcatgccat ttaagttgca aattatccaa
      721 tccatccaag gagagacaca atgaaagcat ccaagaaaac cctggcagtc gtcgtcggca
      781 gcgcgttcgt cgcatctatc gccgccacct cggtcaatgc cgccaccaac ccgttccagg
      841 ccaagaccct gacggccggt tacatgctgg cagacggtag cgacaagatg aaggacggca
      901 agtgcggtac cggcaagtgc ggcgccgaga agaaaaagat gatggaagaa aaggccaagg
      961 aaggctcttg cagcgcggac aaggcgaagg aaggttcctg ccatgccgac aaggccaagg
     1021 aaggctcctg cagcgcggac aaggccaagg aaggctcctg ccacgctgac aagtagtcgt
     1081 tcgatggcgc agactgagaa gatccatggc actggtctgg ggctgaagcg tgagctgatc
     1141 ccccagatcc agtcggcctg ggccgaaggc gggcgtcata acccgctggt ggccaacatc
     1201 gattttctcg agatcgcgcc tgaaaactgg atcggtgccg gtggccgcac agcgaaacag
     1261 ctggcgtggt tcaccgagcg cttccccatg gtttgccatg gcctgtgcct ctcactcggc
     1321 ggaccagacc cgctcaacga accttttttg caacgcgtca aacagttcct cacgcaacac
     1381 catattccgc gctataccga acatctgagc tactgttccg acggtggcta cctgtatgac
     1441 ttgttgccca ttccgttcac ggaagaagca gtgcaccatg tcgctggccg tatacgacgc
     1501 acgcaggata tcctggaacg tcggatcgct gtggagaatg cctccttcta tgcggcagcg
     1561 ccgatctctg agatggatga accgagcttt atccgcgccg tgctcgagga ggctgattgc
     1621 gacctgcatc tcgacctcaa caatatctat gtcaacagcg tgaatttcgg gttcgatccg
     1681 cacgcattcc tgcgcagcat ccctggcgag cgcatcgtct atgcccatgt tgccggccac
     1741 gatcgcgaag cggatgacct ggcgatcgac acgcatggcc aggacgtcat cgacccggta
     1801 tgggcgctgc tgcgagaagc ctatgatttg tacggcgtgt tcccgacctt gcttgagcgc
     1861 gataccaaca tcccgccatt ggagaggctg gtccgcgaga tcgattacat cgccgaactc
     1921 caggcgcccc atcgtcaatc tgcgagagcc gtgctatgac tattcagccg atgcgaacgg
     1981 acgattcgat gccggcattc cagcgttacc agttaagctt caccggccat atccgtgatc
     2041 cgcaacacca gccaaaaccg gacaaggtgg cggcgcggcg catgcgcgtc tataccgaga
     2101 ttgtcttcaa taatctctat ggcgctgtct cggcctgttt tccggtcgcg cagaaggtgc
     2161 tg
//
LOCUS       DATMTW010000092         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_578268, whole genome shotgun sequence.
ACCESSION   DATMTW010000092 DATMTW010000000
VERSION     DATMTW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578268"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN084_01760"
     CDS             complement(<1..389)
                     /locus_tag="VN084_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWT28210.1"
                     /translation="MSVAPDNPRPEDALPDGALIDRYGRKVDYIRLSITDRCDFRCVY
                     CMSEDMTFLPREEVLSLEECARLVKAFVSLGVSKVRITGGEPLVRKNALKLFEEVGQL
                     PGLRELVVTTNGSQLEHQAAALKRAGVR"
     gene            complement(469..2121)
                     /locus_tag="VN084_01765"
     CDS             complement(469..2121)
                     /locus_tag="VN084_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWT28211.1"
                     /translation="MAFELEHDWQGNLATPRARIGESAKTRLLILLCVVWVCLGLISH
                     QPWKPDESQSISIVKQMLAGEHLLTPMAIGQTTIGTPPLYYLSAAGMAKLLSPWLPMH
                     DGARLVSGLWMAITLLMVGMIGRELWGRGIGRQTTFIFLGSLGLIVTAHMLMPEVAAL
                     AGCAMGLYALALAKRRPFRASALLGTGIGVAFLAGDVRYAGIVLCTALALPIFFGHWR
                     SKSFFIVLGLSLIAALPWLSIWPLLLWHLAPVQFEGWLAQSVAQFSQHNHWYFTKTLA
                     WFAWPALPMAAWGLWRFRANLLTKPKFQLLIVNFAVALLLTGFAANDREIFALPLLLP
                     LTALAGGSVETLRRGAAGLLNWFGLILFGVMSFLIWLGWFAMMTGWPAKIAERMQVLS
                     AIAEPHFRLPAFLAALLVTVIWCVVVFNAKRSNRAAVTDWAVGITMTWSLLMSLWLPW
                     IDSARSYEKVMHQIATAAPAGFACITSRNLGAAQQALLHYYTDIQTQPFETTQRMDCD
                     LYLIQDERGRDKIEPGPSWQLIWHGKRPTDRRESFRLYQHLG"
     gene            complement(2239..2439)
                     /gene="rpmE"
                     /locus_tag="VN084_01770"
     CDS             complement(2239..2439)
                     /gene="rpmE"
                     /locus_tag="VN084_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWT28212.1"
                     /translation="MKADIHPNYPDVTVTCSCGNKFKTRSTSGRDLNIEVCSQCHPFY
                     TGKQKIMDTAGRVEKFRQKYGM"
BASE COUNT          552 a          743 c          842 g          517 t
ORIGIN      
        1 cgaacgccgg cccgcttcag tgcggcggct tgatgctcga gctgcgaacc gttggtggtg
       61 acgacaagct cacgcaggcc tggtagctgg ccgacctctt cgaacagttt cagggcattc
      121 ttgcgcacga gcggctcgcc acccgtgatg cgtactttgc ttacgccaag agagacaaag
      181 gctttcacca ggcgcgcgca ttcctcgagt gacagtacct cttcgcgcgg caaaaaggtc
      241 atgtcctcgg acatgcagta cacgcaacga aaatcacagc gatcggtgat cgacagacgg
      301 atgtaatcca ccttgcgacc gtaacggtcg atcagtgcgc catctggcag tgcgtcttct
      361 gggcgtgggt tatcgggggc tactgacatg cggggatacc ggccttgaat ccattggtat
      421 agacaatcga ttgtaagtct ttagggatgg atgaacaatg tcaatcgcct agccgaggtg
      481 ctggtagagg cggaaacttt cgcgtcggtc tgtcgggcgc ttgccatgcc agatcagttg
      541 ccaggacggc cctggctcga tcttgtcgcg gccgcgttcg tcctggatca agtagaggtc
      601 gcaatccatg cgctgcgtcg tctcgaacgg ctgcgtctgg atatcggtat agtaatgcag
      661 cagcgcttgc tgcgcggcgc ccaggttgcg gctggtgatg caggcgaatc cagctggcgc
      721 ggcagttgcg atctggtgca tgactttctc gtaacttctc gctgaatcga tccaggggag
      781 ccagagcgac atcagcaggc tccatgtcat ggtgatgccg actgcccaat ccgtgactgc
      841 ggcacggttg gaacgtttgg cgttgaacac gacgacacac cagatcaccg tgaccagcaa
      901 ggccgcgagg aatgccggca gacggaagtg tggctcggcg atcgcggaca ggacttgcat
      961 gcgttcggcg atcttcgccg gccagccagt catcatcgcg aaccagccga gccagatgag
     1021 gaaactcatg accccgaaca ggatcaggcc gaaccagttg agcaagccgg cggcgccgcg
     1081 ccggagggtc tcgacactgc cccccgccag cgccgtgagc ggcaacagca gcggcagggc
     1141 gaagatctcg cgatcgttgg ctgcgaagcc agtgagcaac aaggcgacgg cgaaattgac
     1201 gatgaggagc tggaacttgg gcttggtgag caagttggca cggaagcgcc acaggcccca
     1261 ggctgccata ggcagtgcgg gccaggcgaa ccaggccagg gtcttggtga agtaccagtg
     1321 gttatgctgg ctgaattgcg cgacggactg cgctagccag ccctcgaact gcactggcgc
     1381 cagatgccag agcagcaaag gccagatcga cagccagggt agcgctgcga tcaggctcaa
     1441 gccgagcacg atgaaaaaac tcttgctgcg ccaatggccg aaaaagatcg gcagggccag
     1501 cgccgtgcac aagacgatgc cggcatagcg cacgtccccc gccaggaaag cgacgccgat
     1561 acctgtcccg agcaaggcac tggcgcggaa cggcctgcgt ttggccaagg ccagggcata
     1621 gaggcccatg gcgcatcccg ccagtgcggc gacttccggc atcagcatgt gggcggtgac
     1681 gatcagccct agtgacccga gaaagatgaa tgtcgtctgg cgcccgatgc cacgccccca
     1741 tagttcccgg ccgatcatgc cgaccatgag cagggtgatc gccatccata gcccactgac
     1801 cagccgcgca ccatcatgca tgggtagcca gggggagagc agcttggcca tgccggccgc
     1861 gctcagatag tagagcggcg gagttccgat cgtggtctgg ccgatggcca tcggcgtcag
     1921 caaatgttcg ccggccagca tctgcttgac gatgctgatg ctctgggatt cgtctggctt
     1981 ccagggttgg tggctgatga ggcccaggca gacccagacc acgcacagca ggatcagcag
     2041 gcgcgtcttg gcgctttcgc cgatccgtgc gcgtggggtc gccagattgc cttgccagtc
     2101 atgttcgagt tcgaatgcca cgagatcctt attttctagt gcctagcccg ttttgggtct
     2161 gcgtattcta atgcgcgggg cgatcaacat aaaacaaaaa ggcagcctga gctgcctttt
     2221 tcgaacgctg aatagcgatt acatgccgta tttttggcgg aacttctcaa cgcgaccggc
     2281 ggtatccatg atcttctgct tgccggtata gaacggatgg cactgggagc agacttcgat
     2341 gttgaggtcg cggccgctgg tggagcgggt cttgaacttg ttgccgcaac tgcaggtgac
     2401 agtcacgtca ggataatttg gatgaatatc ggctttcatg ctcgtgtctc tttcagttgc
     2461 ttttagcgaa gggcgcaatt atcaatgaaa tgagggaatt aatcaacctc tgcgcatcga
     2521 gtcgaaaaag tcggcgttgt tcttggtcgc cttgatcttg tcgagcagga attccatggc
     2581 tgaggggtag aggctgcgac acggtttcgc gtagcgcggt gcgggagcag cctcgagtga
     2641 cctcgaaacg gatc
//
LOCUS       DATMTW010000093         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_141620, whole genome shotgun sequence.
ACCESSION   DATMTW010000093 DATMTW010000000
VERSION     DATMTW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141620"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VN084_01775"
     CDS             <1..386
                     /locus_tag="VN084_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWT28213.1"
                     /translation="VEKYGKWVLISHHDIRLADQWFDRYGEIIIFVGRLLPAVRTFIA
                     FPAGIARMPMGKFIAYTFIGSLIWCWLLAYAGMKTGEHWESLKVYFHQFHIVLVVAAV
                     IFVVWYVRRHLRAIRRLHLHDDDPA"
     gene            complement(411..3857)
                     /locus_tag="VN084_01780"
     CDS             complement(411..3857)
                     /locus_tag="VN084_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWT28214.1"
                     /translation="MLREDAHSREQALAPDSFIVEAPAGAGKTELLTQRYLKLLQQVD
                     DPEEIIAITFTNKAASEMRVRILDSLQLAANGVSPAQPHKQVTYKLGLKALQRSADRG
                     WRLLDNPSRLRIYTIDSLSSNLARQMPLLSRFGAQPGVRDDASLYYEEAATNTLDLLE
                     DPQAGEIIRRALQYFDNDTYKLGGLLANMLRMRDQWLNYTQGQATAEAAEAALQQMLG
                     QDLQAAAHYLSPRIQQALMPIARYAASNLACEEPIALLRDWETVIPPEPIALPMWRAL
                     ADLLLTGSDHKGGLRKALNKNQGLPATDAAKPYKAQLTEILDTLRDTPGAERAIARLR
                     LLPEARHGDEAWQIIATLGDLLNIAVAQLWFVFQAHGEVDFVEIAQRALRALKDDTDS
                     PTDLALKLDYRIQHLLVDEFQDTSPTQIKLLKCLTSGWVPGDGRTLFLVGDPMQSIYR
                     FRKANVGLFLSVAREGIGDVALTPLRLWRNNRSCPPVVDWVNHAFSGVFPAQDSVRQG
                     AIAYRPFVATKANETDEGVQVHALVSPRGDDANQEQREGSEEDEATESSADSLNDVRQ
                     REANVIIDIIQQTRAQDPKRKIAVLVRARNHLHALVTEIRRNHPNLSFQAVEIEELAN
                     RQIVQDHLSLTNALHHRADRLHWLAILRAPWCGLTLADLDQLVGGDRYSTIPSLMEDE
                     GRLERLSDDGRARLLHLREVMQTALAQRGRQRISRWVHGVWLMLGGPDCLWEAGDVRD
                     VQAFFARIAQLEATGQFTTELLATEVKKLYAAPDTEADERLQFMTIHKSKGLEFDTVI
                     LPGLDRKTGNGDQPLLLWEEVPREAGHDASQVDLIAAPVTPQGSGRQGPTLYSYLPQL
                     ESERALNEDARVLYVAVTRAERCLHLVGVATPNSKGELKPAKGTFLELLWPQVHMHFA
                     ETAARPVEVGARMESSVALADFVPQLVRLASPGLPALLRSPTAVAVAPSRRATADAGA
                     SAAPDNETNTQIGILLHRYVELIAREGLAAWPLERVASLKAAMRHWLARQGVAADAIE
                     PALQRVIEGLRATLQSEDGQWVLQARETGSVEQTLISDGADGAKQHVMDRTFIEQGVR
                     WIVDYKSTLLDLSIAPQALQAVAEQHRPQLARYAALFEEEGLPVRTAVFFLSIGRLVE
                     LA"
BASE COUNT          631 a         1252 c         1235 g          742 t
ORIGIN      
        1 tggtggaaaa atacggcaag tgggtgctga tctcgcacca tgacatccgg ctggccgatc
       61 agtggttcga ccgctatggc gagatcatca tcttcgtcgg ccgcctgctg ccggcggtgc
      121 gcaccttcat cgccttcccc gccggcatcg cgcgcatgcc catgggcaag ttcatcgcct
      181 ataccttcat cggttcgctg atctggtgct ggctgctggc ctacgccggc atgaagacgg
      241 gcgagcattg ggagagcctg aaggtgtact tccaccagtt ccatatcgtg ctggtggtgg
      301 ccgccgtcat cttcgtcgtc tggtacgtac gccgccatct gcgggccatc cgtcgcctgc
      361 acctgcatga cgacgacccg gcctgaggct tggactggcc tagctaaaga tcaggccagc
      421 tcgaccagcc tgccgatact gaggaagaac acggccgtcc gcaccggcag gccttcttcc
      481 tcgaatagcg cggcatagcg cgcgagttgc ggccgatgct gctcggcaac ggcttgcaag
      541 gcctgcggcg cgatcgatag gtcaagcagc gtcgatttat agtcgacgat ccagcgcacg
      601 ccctgctcaa tgaaggtacg gtccatcacg tgctgtttcg cgccgtcggc gccatcgctg
      661 atcagcgtct gctccacact cccggtttct cgcgcttgca agacccactg gccgtcttcg
      721 ctctgcagtg tagcgcgcag cccttcgatg acgcgctgca gggccggctc gatcgcatcg
      781 gccgcgacgc cctgacgtgc cagccagtgc cgcatggcgg cctttaacga cgccacgcgt
      841 tcaagcggcc aggcggccag gccttcgcgg gcgataagtt cgacatagcg atgcagcaag
      901 ataccgatct gggtattggt ctcgttatcc ggcgcagcgc ttgcgccggc atcggcagtt
      961 gcccgtctcg agggcgccac cgcgaccgcg gtcggcgatc gcagcagcgc cggtaatccg
     1021 gggcttgcca atcgcaccag ctgaggcacg aaatccgcca gcgccacgct actttccatc
     1081 cgggcaccga cttcgaccgg acgcgcggcc gtttccgcga aatgcatgtg cacctgcggc
     1141 cacagcaact cgaggaacgt gcctttggcc ggcttcagct cgcccttgct gttgggggtc
     1201 gccacaccga ccaggtgcag gcaccgttcg gcgcgtgtaa cggcgacgta gagcacccgg
     1261 gcatcctcgt tcagcgcacg ctcgctctcc agctggggca ggtagctata gagggtcggc
     1321 ccttgtcggc cgctaccctg tggtgtcacc ggcgcggcaa tcaggtccac ctggctcgcg
     1381 tcgtgccctg cttcgcgcgg cacctcttcc catagcaaga gcggctggtc gccattaccg
     1441 gtcttgcggt ccagacccgg caggatcacg gtatcgaatt ccagcccctt ggacttgtgg
     1501 atggtcatga actgcaggcg ttcgtcggct tcggtatctg gcgcagcata gagcttcttc
     1561 acctcggtcg ccagcaactc ggtcgtgaat tgcccagtgg cttcgagttg cgcgatccgc
     1621 gcgaagaacg cctgcacatc gcgcacgtcg cctgcttccc acaggcaatc cggcccgccc
     1681 agcatgagcc agacgccgtg cacccaacgg ctgatacgct gccggccgcg ctgcgcgagc
     1741 gcggtctgca tcacttcccg cagatgcagg agccgtgcgc gaccgtcatc gctgagtctc
     1801 tccagtcggc cttcatcttc catcaggctg gggatggtgc tgtagcggtc gccgccgacc
     1861 agctggtcga ggtcggccag cgtcaaaccg caccagggtg cgcgcaagat cgccagccaa
     1921 tgcaagcggt cagcgcgatg gtgcaaggcg ttggtcagcg acaggtggtc ctgcacgatc
     1981 tggcggttgg ccaattcctc gatctcgacc gcctggaaac tgagattggg atggttgcgg
     2041 cggatctcgg tcaccagcgc gtgcaaatgg ttacgcgcac gcaccagcac ggcgatcttg
     2101 cgttttgggt cctgcgcgcg cgtctgctgg atgatgtcga tgatgacatt agcctcgcgt
     2161 tgccgcacat cgttgaggct atccgccgaa ctctccgtcg cctcgtcttc ctcgctaccc
     2221 tcgcgctgct cctggttcgc gtcatcgccc cggggcgaga cgagggcatg cacctgcaca
     2281 ccctcatcgg tctcgttcgc cttggtggcg acgaacggac gataagcgat ggcgccctgg
     2341 cgcacgctat cctgcgccgg aaagacgccc gagaaggcat ggttgaccca gtccaccact
     2401 ggcgggcagg aacggttgtt acgccacaac cgcagtggcg tcagggcgac atcgccgatg
     2461 ccctcacgcg ccacgctgag gaacaggccg acgttggcct tgcggaagcg gtagatgctc
     2521 tgcatggggt cgcccactag gaacaaagta cggccgtcgc caggcaccca gccgctggtc
     2581 aggcatttga gcagtttgat ctgggtcggg ctggtgtcct ggaactcatc caccagcagg
     2641 tgctggatgc ggtaatcaag cttgagcgcg agatcggtcg ggctgtcggt atcgtccttg
     2701 agggcgcgta gcgcacgctg ggcgatctcg acgaaatcca cctcgccatg cgcctggaat
     2761 acgaaccaga gctgggcaac ggcgatattg agcaggtcgc cgagcgtggc gatgatctgc
     2821 caggcctcgt cgccgtgccg cgcctccggt aacaggcgca ggcgcgcgat ggcccgttcg
     2881 gcacccggtg tgtcgcgcag cgtatcgagg atctcggtga gctgcgcctt gtaaggcttg
     2941 gctgcgtcgg tcgctggcaa gccctgattc ttgttgagcg ccttgcgcaa accgcccttg
     3001 tggtcgctac cggtcagcag caggtcagcc agcgcacgcc acatcggcag cgcgatcggc
     3061 tcaggcggga tcacggtctc ccagtcacgc agcagggcga tcggctcctc gcacgccagg
     3121 ttgctcgccg cataacgggc gatcggcatc agggcctgct ggatgcgtgg gctcagatag
     3181 tgggcagcgg cctgcaaatc ctggcccagc atctgctgca gcgccgcttc tgcggcttcg
     3241 gccgtggcct gaccttgggt gtaattgagc cactggtcgc gcatgcgcag catgttcgcc
     3301 agcaagccgc cgagtttgta ggtgtcgtta tcgaaatact gcagtgcccg gcggatgatc
     3361 tcgccggcct gcgggtcttc cagcaggtca agcgtattgg tcgcggcttc ctcgtagtag
     3421 aggctggcgt cgtcacgcac gccaggctgc gcgccgaaac ggctgagcaa cggcatctgc
     3481 cgcgccaggt tgctgctcag cgaatcgatg gtgtagatgc gcaggcgcga aggattgtcc
     3541 agcaggcgcc agccccggtc ggcactgcgc tggagggctt tcagacccag cttgtaggtc
     3601 acctgcttgt gcggctgcgc gggtgaaacg ccgttcgctg cgagttgcag actatcgagg
     3661 atgcgcacgc gcatctcgga ggccgccttg ttagtgaaag tgatcgcgat gatctcttcc
     3721 ggatcgtcca cctgctgcaa taacttgagg tagcgctggg tcaatagttc ggtcttgccg
     3781 gctccggcgg gcgcctcgac gatgaacgaa tccggcgcca gcgcctgttc gcggctgtgc
     3841 gcatcttccc tgagcagcgc
//
LOCUS       DATMTW010000094         1699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_578584, whole genome shotgun sequence.
ACCESSION   DATMTW010000094 DATMTW010000000
VERSION     DATMTW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578584"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VN084_01785"
     CDS             <1..865
                     /locus_tag="VN084_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT28215.1"
                     /translation="IAPIKSLTSMNEDLQIGLAAAQTVFAMIDGKTERSEGTLTIARA
                     KGELEFRGVTIKYDNAKRNAISNLNIVVHPGEKLALVGRSGAGKTTLVNLLPRFYELQ
                     TGLILLDGVDIRAMPIDNLRQQFALVSQDVILFNDTVFNNIAYGMLGHATEAEVIAAA
                     KAAHAWEFIQHLPGGLQSEIGDRGVRLSGGQRQRLAIARAILKDAPILLLDEATSALD
                     TESEQHVQAALDELMRNRTSIVIAHRLSTIENADRILVMEQGQIIESGSHQDLLAAEG
                     HYAKLYHKQFH"
     gene            874..>1699
                     /gene="lpxK"
                     /locus_tag="VN084_01790"
     CDS             874..>1699
                     /gene="lpxK"
                     /locus_tag="VN084_01790"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWT28216.1"
                     /translation="MADWLQRQWYRISIWHLVLIPISWVFGLVIILRREAYRIGWLAS
                     CKLPIPVIVVGNITVGGTGKTPLVVWLAQQLQAMGFHPGIISRGYGAEHRQPMPVHAD
                     SDARALGDEPVLIAMRTGLPVWVCRDRVASARALRQAHPACDVIISDDGLQHYRLQRD
                     VEIAVIDGQRLFGNRQLLPAGPLREPPSRLKEVDAIVCNGGFSPADGHVYDMRLNAGS
                     FRQVRNPARTASVSDLEQKTLLAIAGIGHPERFFAQLREMQLVFESRAFPDHHPYVA"
BASE COUNT          338 a          513 c          508 g          340 t
ORIGIN      
        1 gatcgcgcca atcaaaagcc tgaccagcat gaatgaagat ttgcagatcg gtctcgctgc
       61 ggcgcagacc gtattcgcga tgatcgacgg caagacagag cgtagcgagg gcacgctgac
      121 gatcgcgcgc gccaagggcg aactggagtt ccgcggagtc acgatcaaat acgacaacgc
      181 caaacgcaac gcgatcagca acctcaatat cgtcgtccat cccggcgaga agcttgccct
      241 ggtagggcgc tctggggcgg gtaaaaccac cttggtcaac ctcttgcccc gcttctatga
      301 gctgcagacc gggctgatcc tgctggatgg tgtcgatatc cgtgccatgc cgatcgataa
      361 tctgcgccag cagttcgcgc tggtgagcca ggatgtcatc ctgttcaacg acactgtctt
      421 caacaacatc gcttacggca tgctcggcca tgctacggaa gccgaggtga tcgccgctgc
      481 caaggcggcc catgcctggg aattcatcca gcatctgcca ggcggattgc agagcgagat
      541 cggcgaccgt ggcgtgcggc tctccggcgg ccagcgccag cgactcgcca tcgcgcgagc
      601 catcctcaag gatgcgccca tactgctgtt ggatgaggcg acctcggcgc tcgataccga
      661 atctgaacag catgtacagg cggcgctgga tgagctcatg cgcaaccgta catccattgt
      721 catcgcccat cgtctgtcga cgatcgagaa tgcggaccgt atcctggtca tggaacaggg
      781 ccagatcatc gagagcggtt cgcatcaaga cttgctggcg gctgaagggc attacgcgaa
      841 gctctaccac aagcaattcc actaggtgac gccatggccg actggctgca gagacagtgg
      901 taccgtatca gcatctggca ccttgtgctg atccccatct catgggtctt tggtctcgtc
      961 atcatcttgc gtcgcgaagc gtaccgcatc ggctggctgg cttcctgcaa actccccata
     1021 cctgtcatcg tggtcggtaa catcaccgtc ggcggcacag gcaagacgcc gctggttgtt
     1081 tggctcgcgc agcaactgca ggccatggga ttccatccgg gaattatcag ccgcggttac
     1141 ggcgcagagc atcgtcagcc gatgccggtg catgccgata gcgatgcacg tgcgctgggt
     1201 gacgaaccgg tgctgatcgc catgcgtact ggcctgcctg tctgggtttg ccgggatcgg
     1261 gtcgcgagcg caagggccct gcgtcaggcg catccggctt gcgatgtgat catcagtgac
     1321 gatggcttgc agcattaccg gctgcagcgc gatgtcgaga tcgccgtgat agacggccag
     1381 cgcctttttg gtaatcgtca gctcctgccc gcgggtccat tgcgagaacc gccatcgcgg
     1441 ctgaaagaag tcgacgccat cgtttgcaac ggcggtttca gtccggcgga tgggcatgta
     1501 tatgacatgc gactgaatgc cggtagcttc cgtcaggtgc gtaacccggc ccgtacggcc
     1561 agcgtatccg atcttgaaca gaagacatta ctggctatcg ccggtatcgg acatccagag
     1621 cgcttcttcg cacagctgcg cgagatgcag cttgtattcg agtcccgcgc gttcccggat
     1681 caccaccctt atgtcgccg
//
LOCUS       DATMTW010000095         7178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_410589, whole genome shotgun sequence.
ACCESSION   DATMTW010000095 DATMTW010000000
VERSION     DATMTW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410589"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VN084_01795"
     CDS             complement(<1..724)
                     /locus_tag="VN084_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWT28217.1"
                     /translation="MNLTDTLEIVRVTDVGQRRDHNEDAVASDSEIGLVILADGMGGY
                     NAGEVASEIAVLTLVAELKESMATLAPGHVDPASGMQAESQLLLDAVAKANASIYEVS
                     QSQPQCAGMGTTLVAAMFTNGKLLVGHIGDSRMYRLREESLEQITEDHSLLNEQIKSG
                     LITPEQARYSSNKNLVTRALGIDPEVELELHEHDVEVGDLYLLCSDGLSDLVEDEEIA
                     LTLTSLSANLELAANQLVQMAND"
     gene            complement(728..3259)
                     /locus_tag="VN084_01800"
     CDS             complement(728..3259)
                     /locus_tag="VN084_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWT28218.1"
                     /translation="MKKTFWKSDWFAGLIVSLLFLFLGGGALLQGLERKAYDLGVQGS
                     TRNAGDKIAIIAIDDQSIANIGRWPWSREIHAKMIDMLAGAKAKVIGNTVFFIDPQVD
                     PGLAYINKLAEFVASSSIPTTAPEAGQLDSMLKDAQAALNTDTKLAQSVQQAGNVVLA
                     MPFVVGEPQGNPDKPLPDYVSRNALTKIMDRVNAEANGMLPIPTISALPPIPEIGEQA
                     AAIGHLNANPDVDGAIRAEPLVLAYYDQYYPSLSLQIAARYLNLKPADIQPRLGEGVR
                     LGKLNIATDPFLQMHTFFYADHDGRSVFPVDSFYDVYSGKIPVEKYRDRIVLIGATAG
                     GIGDSQITPISSGMKPIETLAHSVASILNEDFFVVPAWAAWAERGVWLLVALYLIVLL
                     PRLNAGMAAAITALLLIALLAVSYILLTQEGMWLQLVTPAVLLVVGHALLTTKRFLLT
                     EKGKIRSEAESAESNRNLALMFQSQGQLDMAFDRLRKCPMDDSVMDVLYNLALDFERK
                     RQFNKAESVFGYMADFNPKYKDLEQRLARSKAMAETVILGGAGGGRTNSGTMILNDGT
                     VEKPMLGRYQVEKELGKGAMGVVYLGKDPKIGRVVAIKTMALAQEFEPDELQEVKERF
                     FREAETAGRLSHPNIVTMYDAGEEHDLAYIAMEFLKGKDLAPYTKPDNLLPLAKVMDI
                     VAKTADALDYAHGNNVVHRDIKPANIMYEVDTGVVKVTDFGIARITDSSKTKTGMVLG
                     TPSYMSPEQLAGKKVDGRSDLFSLGVMLYQMSTGQLPFTGDSMATLMFKIANETHAGV
                     KTINPELPQCLSDIIDRMLEKDIDKRYARGAEIAADLRRCAEQLG"
     gene            complement(3495..3965)
                     /gene="nusB"
                     /locus_tag="VN084_01805"
     CDS             complement(3495..3965)
                     /gene="nusB"
                     /locus_tag="VN084_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWT28219.1"
                     /translation="MSTEQKPAAKPKASRNRRKSRELVLKGIYRSLINQADPKLVMRD
                     LADDPDFERADQDYLRQLLAGVLQHMPELDTRLGSFLDRTAAELSPIEHAILYLSAFE
                     LIHDPSIPYRVAINEGVELAKLYGGIDGHKYVNGVLDKLAAEARPSEVKRTSRD"
     gene            complement(3962..4411)
                     /gene="ribH"
                     /locus_tag="VN084_01810"
     CDS             complement(3962..4411)
                     /gene="ribH"
                     /locus_tag="VN084_01810"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWT28220.1"
                     /translation="MKKLDLNLDGKGMRIGIVLSRFNPEIGHGLLAACEAELLKLGVA
                     ADDIILATVPGALETPVVLHQMADSDEFDALIALGAIIRGETYHFEVVANESARGVSD
                     VQLSTGIPVANAILTTEDDDQAIARMAIKGAEAATVAVEMANLLRQL"
     gene            complement(4540..5097)
                     /locus_tag="VN084_01815"
     CDS             complement(4540..5097)
                     /locus_tag="VN084_01815"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWT28221.1"
                     /translation="MTRCRWAKTPLDIAYHDAEWGVPVHDERRLFEFLILEGAQAGLS
                     WHTILQKRARYREVFDGFDPAKVADYGDAKVSSLLTDAGIVRNRLKVAAAIGNARHFL
                     EVQAERGSFDAYLWDFVDGQPIINTWRSHEDIPAKTAISDQLSRDLQKRGFKFVGSTI
                     CYAMMQAVGMVNDHTLDCFRYADLA"
     gene            5178..5870
                     /gene="phoB"
                     /locus_tag="VN084_01820"
     CDS             5178..5870
                     /gene="phoB"
                     /locus_tag="VN084_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HWT28222.1"
                     /translation="MPANILVVEDEPAIQELLALNLTQAGHNAIRALSVEQAQKLIRE
                     ALPDLIILDWMLPGMSGIEFARKLKSDELTKTIPIIMLTARGEEADKVRGLEVGADDY
                     VTKPFSPRELNARIKAVLRRRAPQMTDDPIEVSGLRLDPMTHRVSGNGTTLDLGPTEF
                     RLLHYLMSNPERVHSRSQVLDRVWGDRVFVEDRTVDVHIRRLRRALSESGHEDLIQTV
                     RGVGYRFSALAS"
     gene            5919..>7178
                     /gene="phoR"
                     /locus_tag="VN084_01825"
     CDS             5919..>7178
                     /gene="phoR"
                     /locus_tag="VN084_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HWT28223.1"
                     /translation="MQDIRWRAFWLGLVAVLVCLVAWALTDALTALIIFTCFVTLYLA
                     SHLYWLHQLLVWFKKPELSTMPLGSGIWEDVFAAIYHEQRRHSRSQTQLSSALDRFRH
                     AASALPDGVVLLNGSDQIEWCNPPAESQLGLSMKQDAGQPIVYLVRHNEFIQYLQAQD
                     FSEPIKLKSWRNTEVTLEIQLVPFGANQKLLICRDITQMEKVETMRRDFIANVSHELR
                     TPLTVVGGFLETLNDIEGAVPENTRGYFNMMQEQTARMRRLIEDLLTLSQLENSQTVP
                     NDSEIDMTALMSMLLNDAQGLSNGRHHLSLDADPNLSLTGASEELHSAFGNLISNAIR
                     YTPEGGDIVIRWERRGQEAVFSVKDTGIGIEQQHIDRLTERFYRVDRSRSRETGGTGL
                     GLSIVKHILTRHQARLEIESVPGQGSTF"
BASE COUNT         1438 a         2206 c         2087 g         1447 t
ORIGIN      
        1 tgtcattggc catctgcacc agctgattcg ccgccagctc aagattagca cttaatgaag
       61 tcagcgttaa tgctatctcc tcatcctcca ccaggtcgct caggccatcc gaacacaaca
      121 ggtacaggtc gccaacttcg acatcgtgct cgtgcaactc gagctcgacc tctgggtcga
      181 tgcccagcgc gcgcgtgacg aggtttttgt tgctggagta ccgggcttgc tcaggcgtga
      241 tgagcccgga tttgatctgc tcgttgagca gggagtgatc ttccgtgatc tgctccagcg
      301 actcctcgcg caaccggtac atgcgcgaat caccgatatg cccgaccagc agcttaccgt
      361 tggtgaacat ggccgcgacc agcgtcgttc ccatgccagc acactgtggc tggctctgcg
      421 agacctcata gatggaggcg ttggccttgg ctacggcgtc cagcagcagc tgactctcgg
      481 cctgcatgcc actggcaggg tcgacatggc caggcgccag cgtcgccatg ctttccttga
      541 gctccgcgac cagggtcaat accgcgatct cgctcgccac ctcgcccgcg ttgtagccgc
      601 ccataccatc ggccaggatc acgaggccga tctcgctatc gcttgcgacg gcatcttcgt
      661 tatggtcgcg ccgctggccg acatcggtga cgcgaacgat ctcaagggta tcggtgaggt
      721 tcatggctca gcccagctgt tccgcgcaac gccggagatc tgctgcgatc tcagcgccac
      781 gggcatagcg tttatcgatg tccttttcca gcatgcggtc aatgatatcc gacaggcact
      841 gcggcagctc cggattgatg gtcttcacgc ccgcatgggt ctcgttggcg atcttgaaca
      901 tcagcgtcgc catcgaatcg ccggtgaatg gcagttgccc ggtgctcatc tggtagagca
      961 tgacgccgag cgagaacagg tcggagcgac cgtcaacctt tttgccagcg agctgttcag
     1021 gcgacatgta gctcggtgtg cccagcacca tgccggtctt ggtcttggag gaatcggtga
     1081 tgcgcgcgat accgaagtca gtgaccttga ccacgccagt atcgacttcg tacatgatgt
     1141 tggctggctt gatatcccga tgcacgacat tgttgccgtg cgcgtaatcc agcgcgtcgg
     1201 cggtcttggc gacgatatcc atgaccttcg ccagcggcag caggttgtcc ggcttggtat
     1261 agggcgcgag gtccttgccc ttgaggaact ccatggcgat ataggcaaga tcgtgctcct
     1321 cgccagcatc gtacatggtg acgatattgg ggtggctgag cctgccggcg gtctccgctt
     1381 cgcggaaaaa gcgttccttc acctcttgca gttcatccgg ctcgaactcc tgtgccagtg
     1441 ccatggtctt gatcgccacc acacggccga tctttggatc cttgccgagg tagaccacgc
     1501 ccattgcacc cttgcccagc tctttttcca cctggtaacg gccgagcatg ggtttttcca
     1561 ccgtaccgtc gttaaggatc atggtgcctg aattggtacg gccaccgcct gccccgccaa
     1621 ggatgacggt ttccgccatg gctttggagc gtgccaggcg ctgttccaga tccttgtact
     1681 tgggattgaa gtccgccatg tagccgaaca ccgattctgc cttgttgaac tggcgcttgc
     1741 gttcgaaatc gagcgcaagg ttgtacagga cgtccatgac gctgtcgtcc atggggcatt
     1801 tgcgcaagcg atcgaaggcc atgtcgagct ggccctggct ctggaacatg agtgccaggt
     1861 tgcggttact ctcggccgat tcggcttcgg atctgatttt gcctttctcg gtcaggagga
     1921 agcgcttcgt ggtcagcaag gcgtggccga ccaccagcag cacggccggg gtcaccagtt
     1981 gcagccacat gccttcctga gtgagcagga tatagctgac ggccagcagg gcgatcagca
     2041 gcagcgcagt gatcgccgcc gccatgccag cgttgaggcg cggcagcagc acgatgaggt
     2101 agagcgcgac cagcagccag acgccgcgtt ccgcccaagc cgcccaggcc ggtacaacga
     2161 agaaatcttc attgaggatg ctggcgaccg aatgcgccag ggtctcgatc ggtttcatgc
     2221 ccgacgagat cggcgtgatc tggctgtcgc cgataccacc agcggtcgcg ccgatcagca
     2281 cgatacggtc acggtatttc tctaccggga tcttgccact gtagacgtca tagaacgaat
     2341 ccaccggaaa taccgagcgc ccgtcatgat cggcataaaa gaaggtatgc atctgcagga
     2401 acgggtccgt ggcgatgttg agcttgccga gcctgacgcc ctcgcccaga cgcggctgga
     2461 tatcggccgg tttcaggttg agatagcggg cagcgatctg taacgacagt gaagggtaat
     2521 actggtcgta gtaggccagc accagcggtt cggcccgaat ggcgccgtcc acatccggat
     2581 tggcgttcag atgaccgatg gccgctgcct gctcgccgat ctccgggatt ggtggcaagg
     2641 cggaaatggt cgggatcggc agcatgccgt tagcttcggc attcacgcga tccatgatct
     2701 tggtcagcgc gttacggctg acatagtcag gcaatggctt gtctgggttg ccttgcggct
     2761 cgccgacaac gaacggcatc gccagcacga cattaccggc ctgctgtacg ctctgcgcca
     2821 gcttggtgtc ggtattgagc gcagcttgtg catctttcag catgctatcg agctggcccg
     2881 cttccggtgc ggtggtaggg atggaagaac tggcgacgaa ttcggccagc ttgttgatgt
     2941 aggcgagacc cggatcgacc tgcggatcga tgaagaacac cgtgttgccg atcaccttgg
     3001 ccttggcgcc ggcgagcatg tcgatcatct tggcgtggat ctcgcgcgac cagggccagc
     3061 gaccgatatt ggcgatgctc tggtcatcga tggcgatgat ggcgatcttg tcgccggcat
     3121 tgcgcgtcga gccttgcacg ccgaggtcat aggccttgcg ttccagcccc tgcaagagcg
     3181 ctccgccacc caggaacagg aacaatagag agacgatgag ccccgcgaac cagtcggact
     3241 tccagaaagt ctttttcaac ccgctcccct ttatcgatcg actgcaatag atacgccagg
     3301 ctggcctgac acacgcaggt gatggccgat atcacacgca ccctgctgca tgccccttat
     3361 tcgcgacgga attatacata cagccggcaa aaacgatatt acacgcgatt tttttcaaaa
     3421 agaagtgcag acggacaaaa gcccgcactt ccgccgcatt cggcaaaaag cacgggcttg
     3481 cgtgaagcga agggtcagtc gcgggaggtg cgcttgactt cgctggggcg ggcttcggcc
     3541 gccagcttgt cgagaacgcc gttgacatac ttgtggccgt cgatcccgcc gtagagcttg
     3601 gcgagctcga ccccttcgtt gatcgcaaca cgatagggga tgctcggatc gtggatcagc
     3661 tcgaacgccg aaagatagag gattgcatgc tcgatcgggc tcaattcggc cgcggtgcgg
     3721 tcgagaaagc tgccaaggcg tgtatcgagt tctggcatgt gttgcagcac acccgccagt
     3781 agctggcgca agtaatcctg gtcggcgcgc tcgaaatccg ggtcatctgc cagatcccgc
     3841 atcaccagct tcgggtcggc ctgattgatc aggctacggt agataccctt gagcacaagc
     3901 tctcgcgact tgcggcggtt acggctggcc ttgggcttgg ctgcgggttt ctgttcggta
     3961 ctcataactg tctgagcagg ttagccatct cgaccgcaac ggtcgcagcc tccgcgccct
     4021 tgatcgccat acgggcgatg gcctggtcat catcctcggt ggtgaggatg gcattggcga
     4081 ccgggatgcc ggtgctgagt tgcacatcgg acactccgcg cgcggattca ttggcgacca
     4141 cttcaaaatg gtaggtctcg ccacggatga tggcacccag tgcgatcagc gcatcgaatt
     4201 cgtcgctgtc ggccatctgg tgcaagacca ccggcgtctc aagagcacct ggcacggtcg
     4261 ccaggatgat atcgtcagca gcgaccccca gtttgagcag ttcagcctcg caagccgcca
     4321 gcaaaccatg gccgatctcc gggttgaagc gggacagcac gatgccgata cgcatgcctt
     4381 tgccatcgag atttagatca agtttcttca cgtggtattc cagtcagata aataggtcct
     4441 gcagacctcc gggctggcga gcctgtcccg aaggcggtaa gcgattcatt cggcgacgat
     4501 tttaccacag acgccaccgc ggcttctgca tttgccggct caggcgagat cggcgtagcg
     4561 gaaacaatcc agcgtatggt cgttgaccat gcccaccgcc tgcatcatgg cgtagcagat
     4621 ggtagaaccg acgaatttga acccgcgttt ttgcaaatct cgcgatagct gatccgaaat
     4681 cgcggtcttc gccgggatgt cttcatggga tcgccaggtg ttgatgatgg gctggccgtc
     4741 gacgaaatcc cagagataag catcgaaact accgcgctcg gcctgcactt ccaggaagtg
     4801 ccgagcattg ccgatagcgg cggcgacctt caggcgattg cgcacgatcc cggcgtccgt
     4861 cagcaggctc gagaccttgg cgtcaccgta atccgccact ttggccgggt cgaagccgtc
     4921 gaacacttct cgatagcggg cacgcttttg caggatggtg tgccagctca atccggcctg
     4981 cgcgccttcc aggatcagga actcgaacag acggcgttca tcatgcacag gcacacccca
     5041 ttcagcatcg tgataagcga tatccagcgg ggttttcgcc cagcgacaac gtgtcatatt
     5101 tttctcggtc atcgtcataa aactgtcata tcatcgtcat atagtgcgct ggagtacaaa
     5161 aggaaaagga acatactatg ccagctaata tactcgttgt tgaagacgaa cctgccattc
     5221 aggagctgct tgcactgaac ctgacgcaag ccggccataa cgccatccgg gccctgagcg
     5281 tggaacaggc gcagaaactc atccgcgaag ccctgcccga cctcatcatc ctcgactgga
     5341 tgctacccgg catgagcggc atcgaattcg cgcgcaagct gaagtccgac gaactgacca
     5401 agaccatccc catcatcatg ctcaccgccc gcggcgaaga ggccgacaag gtgcgcggcc
     5461 tggaagtcgg cgctgacgat tacgtgacca aacccttcag cccgcgcgag ctcaatgcac
     5521 gcatcaaagc cgtgttacgc cgtcgtgcgc cgcagatgac cgacgacccg atcgaagtca
     5581 gcggcctgcg gctcgatccg atgacgcacc gtgtctccgg caacggcacc acgctcgacc
     5641 tcgggccgac cgaattccgt ctgctgcact acctcatgtc caacccggag cgcgtgcatt
     5701 cccgtagcca ggtgctcgat cgtgtatggg gagaccgcgt tttcgtcgag gataggaccg
     5761 tcgatgtgca tatccgtcgc ctgcgccgtg cgctctccga atccgggcac gaagacctga
     5821 tccagaccgt acgtggcgtc ggttaccgtt tcagcgccct agcatcgtag gccgctgtgg
     5881 ctcataatag gcacgccgta aattagggaa gaagctgcgt gcaggatatt cgttggcgag
     5941 cgttctggct cggattagtt gccgtactgg tttgcctcgt cgcctgggcg ctgaccgacg
     6001 cgctcaccgc gctcatcatc ttcacctgtt tcgtcaccct ctaccttgcc agccacctgt
     6061 actggctgca tcaactgctg gtctggttca agaagcccga actctccacc atgccgctcg
     6121 gcagcggcat ctgggaagat gtcttcgccg ccatctacca cgaacagcgc cgccacagcc
     6181 gaagtcagac ccagctcagt tccgcgctgg accgtttccg ccacgcggcg agcgccttgc
     6241 ccgacggcgt cgtgctgctc aacggcagcg accagatcga atggtgcaac ccgccagccg
     6301 aatcccagct tggcctctcg atgaaacagg acgcaggcca gcccatcgtc tatctcgtgc
     6361 gccacaatga attcatccag tacctgcagg ctcaggattt ctctgaaccg atcaagctca
     6421 agtcctggcg caacaccgaa gtgacgctgg agatccagct ggtcccgttc ggcgccaacc
     6481 agaaactgct gatctgccgc gacatcacgc agatggagaa ggtcgagacc atgcgccgcg
     6541 atttcatcgc caacgtctca cacgagttgc gcacgccgct gaccgtggtc ggcggcttcc
     6601 tcgagacgct caacgacatc gagggcgccg taccggaaaa tacgcgcggg tatttcaaca
     6661 tgatgcagga gcagacggcc cgcatgcgcc ggctgatcga agatctgttg acgctatcgc
     6721 agctggagaa cagccagacg gtgcccaacg acagtgagat cgacatgacc gcgctcatga
     6781 gcatgctgct gaacgatgcc cagggcttga gcaatggccg tcatcatctc agcctagacg
     6841 ccgaccccaa cctcagcctc accggcgcca gcgaagagct gcacagtgcc ttcggcaacc
     6901 tcatcagcaa cgccatccgc tacacgccgg aaggcggcga tatcgtcatc cgctgggaac
     6961 ggcgcggcca ggaagcggta ttcagcgtca aggacaccgg catcggtatc gaacaacagc
     7021 acatagaccg gctcaccgaa cgcttctatc gcgtcgatcg cagccgatcg cgcgagaccg
     7081 gcggcaccgg cctcggactc tccatcgtca agcacatcct gacccggcat caggctcgcc
     7141 tcgaaatcga gagcgtacct ggccagggca gcaccttc
//
LOCUS       DATMTW010000096         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_309863, whole genome shotgun sequence.
ACCESSION   DATMTW010000096 DATMTW010000000
VERSION     DATMTW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309863"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..440
                     /gene="secG"
                     /locus_tag="VN084_01830"
     CDS             90..440
                     /gene="secG"
                     /locus_tag="VN084_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWT28224.1"
                     /translation="METLVMVVHVLAALGVIGLVLLQHGKGADMGAAFGSGASGSLFG
                     VSGSSNFLSRATAIFVAIFFATSMGLAYIGGHRGEQSSVIKLQQNAVTTSSQPAEKQD
                     AKPAEPSNAKDVPQ"
     gene            475..559
                     /locus_tag="VN084_01835"
     tRNA            475..559
                     /locus_tag="VN084_01835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:509..511,aa:Leu,seq:gag)
     gene            606..962
                     /locus_tag="VN084_01840"
     CDS             606..962
                     /locus_tag="VN084_01840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWT28225.1"
                     /translation="MLENYFPILLFILVGLAVGVAPIVLGKLVAPNKPDAEKNSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLQEIGLFGFIAMMVFLAIL
                     VVGFIYEWMKGALVWE"
     gene            1130..1606
                     /locus_tag="VN084_01845"
     CDS             1130..1606
                     /locus_tag="VN084_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWT28226.1"
                     /translation="MSIEGVLEKGFVTTTLDTVINYTRTGSLWPMTFGLACCAVEMMH
                     AGASRYDLDRFGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR"
     gene            1619..2230
                     /locus_tag="VN084_01850"
     CDS             1619..2230
                     /locus_tag="VN084_01850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWT28227.1"
                     /translation="MSARLKKLEEHLRLALGDQLTRLASAVGELTIECKAADLLSVCQ
                     TLRDHAELKFEQLVDLCGVDYADYGDGRWEGLRFAVVYHFLSVSLNQRVRLRVFAADK
                     DFPVLPTAVDLWPAANWFEREAFDLYGIMFDGHPDLRRLLTDYGFVGHPFRKDFPMIG
                     NVEMRYDPEQQRVIYQPVTIDPRNNVPRVIREEGFHEEGFRNG"
     gene            2223..>2554
                     /locus_tag="VN084_01855"
     CDS             2223..>2554
                     /locus_tag="VN084_01855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWT28228.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAENRTYLQSVPYMDRLDYVSMMSNEHAYVMAIEKLLGLEVPIRAQYIRVMFD
                     EITRVLNH"
BASE COUNT          485 a          703 c          773 g          593 t
ORIGIN      
        1 gtaagtgttc tttgaacgcc ggcgattttg aaggaatttg tcgcgctgct tgcggctaag
       61 ggcatgcaag cagggagatg caggggaaaa tggaaacttt ggtcatggta gtacatgtgc
      121 tcgcggcact gggcgttatc ggcctggtgc tgttacagca tggtaaaggc gcagacatgg
      181 gggcagcgtt cggtagcggc gcttccggca gcctgttcgg cgtcagtgga tcctccaact
      241 ttttgagccg cgctacggct atctttgtgg caatcttttt tgcgaccagc atgggtctcg
      301 cctatatcgg cggccatcgc ggtgagcaat ccagcgtcat caagctgcag cagaatgcag
      361 tgacgacctc ttcgcagcct gctgaaaagc aggatgccaa gcctgccgag ccatccaacg
      421 ccaaggatgt gccgcaataa gctgagtgag tggggttaag cagccccgat gcatgccgac
      481 gtggtggaat tggtagacac gctatcttga gggggtagtg acgaaagtcg tatgagttcg
      541 agtctcatcg tcggcaccaa gttaaataac ctgccagcaa cgacgctggc aattgggatc
      601 tggacgtgct ggaaaattac tttccaatct tgctgttcat tctggtcggc cttgccgtcg
      661 gcgtagcccc tattgtcctt ggtaagttgg tcgcacccaa caagccggac gccgaaaaaa
      721 actcccccta tgaatgcggc ttcgaagcct tcgaagacgc ccgcatgaag ttcgacgtac
      781 gttactacct cgtcgccatc ctatttatcc tgttcgacct tgagatcgcc ttcctcttcc
      841 cctgggctgt cgtgctgcag gagatcggcc tgttcggctt tatcgctatg atggtgttcc
      901 tcgcgatcct tgtggtcggc ttcatctacg agtggatgaa aggggcgctg gtgtgggagt
      961 agcgcgaatg agtcgccggg cttgccaagc tgcgaccaag atgaatgagg cagggtgccg
     1021 aatcggtcat cacgctgttc caggggcttg ccctgcggct tcgatgcttg atggtctttc
     1081 gtctggggtt ttggtttgcg gtttttgccg gaatgatgag gcggtataga tgagcatcga
     1141 aggcgttctc gaaaaaggct ttgtcaccac cacgctggat acggtgatca actatacgcg
     1201 caccggctcc ctgtggccca tgaccttcgg gctggcctgt tgcgcggtgg agatgatgca
     1261 tgccggtgct tcgcgttatg acctcgatcg tttcggcgtc gtgttccgtc caagcccgcg
     1321 ccagtcagat gtgatgatcg ttgccggcac gctggtcaac aagatggcgc cggcgctgcg
     1381 caaggtctat gaccagatgg ccgagccgcg ctgggtcatt tccatggggt cctgtgccaa
     1441 cggcggcggt tattaccatt actcctacgc tgtggtccgc ggctgtgacc gcatcgtgcc
     1501 ggtcgatgtc tatgtgccgg ggtgtccgcc gactgcggaa gccctgctgt acggcatcat
     1561 ccagttgcag aacaagatca agcgcaccaa caccatcgcc cgttgagaac atagatacat
     1621 gagcgcccgt ctgaaaaaac tcgaagaaca cctcaggctt gccctgggcg atcagctgac
     1681 caggctggcc tcggcggtgg gtgagttaac gatcgaatgc aaggcggccg acctgctgtc
     1741 cgtgtgtcag accttgcgtg accatgcgga gctcaagttc gagcagttgg tcgacctctg
     1801 cggtgtcgat tatgccgatt acggcgatgg gcgctgggaa ggtttgcgct tcgctgtcgt
     1861 ctatcatttc ctgtcggtga gcctcaatca gcgcgtgcgc ttgcgcgtgt tcgccgcgga
     1921 taaggatttc ccggtgctgc cgaccgcggt cgatctctgg cctgccgcca actggttcga
     1981 gcgcgaggcg ttcgatcttt acggcatcat gttcgacggc catcccgatc tgcgccgcct
     2041 gcttacagac tatggttttg tcggtcatcc gttcaggaag gactttccca tgatcggcaa
     2101 cgtcgagatg cgctacgatc ctgagcagca gcgcgtcatc tatcagcctg tgacgatcga
     2161 tccgcgcaac aacgtgccgc gcgtcatccg cgaagaggga ttccatgagg aaggcttccg
     2221 caatggctga gattcgcaat tacaccatga acttcggccc gcagcacccg gcggcacacg
     2281 gcgtcctgcg actcgtgctg gagctcgacg gtgaagtcat ccagcgtgcc gatccgcaca
     2341 tcggactgct gcatcgtgcg acggagaagc tcgccgaaaa ccgtacttat ctgcagtcgg
     2401 tgccgtatat ggaccgcctg gactatgtgt ccatgatgtc caacgagcat gcctatgtca
     2461 tggccatcga gaagctgctg gggcttgagg tgccgatccg cgcccaatat atccgggtga
     2521 tgttcgacga gatcacgcgt gtgctcaacc attt
//
LOCUS       DATMTW010000097         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_578728, whole genome shotgun sequence.
ACCESSION   DATMTW010000097 DATMTW010000000
VERSION     DATMTW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578728"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VN084_01860"
     CDS             <1..417
                     /locus_tag="VN084_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HWT28229.1"
                     /translation="KKTCDDYFYLKHRKEPRGIGGIFFDDFNELGFDPSFALQRSVGD
                     AFLNAYLPIVQRRKDMPYGECERDFQAYRRGRYVEFNLVFDRGTLFGLQSNGRTESIL
                     LSMPPVVKWRYDWKPADGSPEARLYTDFLTGRDWLA"
     gene            456..>1823
                     /locus_tag="VN084_01865"
     CDS             456..>1823
                     /locus_tag="VN084_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWT28230.1"
                     /translation="MLAQLFRTKPIVPHTDSGLKKCLTAFDLALLGIGCAIGTGIFVL
                     TGIAAATQSGPAVVLSFVFAGIASAFAAFSYAELAAAVGGSGSAYGYSYVAFGEFFAW
                     VIGWILLLEYGVGAAAVANGWSGYFINTLANFSIHIPTTWTRAPSLGGVINLPAFAII
                     WLLTLLLIAGVKESARFNNIIVVVKLSTIVIFIALASMHLNSDNWHPFMPFGWFDTVE
                     NGKTIGVLAGASLVFFAYFGFDAVSTAADEAKNPQRDLPIGLIASLSFCTIVYILVSG
                     ALTGIVPYTQLNVDSPVAWALSQLGYTWSSTLVATGVLAGLITVLLVLLYGLTRILFA
                     MSRDGLISPVFSKVDPDRQTPVRIILMCGFVVSLVAGFFPLGALAETVNIGTLASFVM
                     VCAGVIVMRMRHPDLHRPFRNPWNPLIPVLGILSCTALMTFLPAGTWHRFLLWVAAGI
                     VFYFSY"
BASE COUNT          310 a          566 c          508 g          439 t
ORIGIN      
        1 aaaaaaactt gcgacgacta tttttatctc aagcatcgca aggaaccgcg cggtatcggc
       61 ggcatcttct ttgacgattt caacgaactc ggctttgacc cgagttttgc gctgcagcgc
      121 agcgtcggcg acgcctttct caacgcctat ctgccgatcg tgcagcgccg caaggacatg
      181 ccctacggtg agtgtgaacg agacttccag gcctatcgcc gcggccgcta tgtcgaattc
      241 aacctggtat tcgatcgcgg tacgctgttc ggcctgcagt cgaacgggcg caccgaatcc
      301 atcctcttgt cgatgccgcc cgtggtcaaa tggcgttatg actggaagcc ggccgacggt
      361 tcgccggaag cgcggctgta tacggatttc ctgacgggcc gcgactggct ggcctgattc
      421 atgcatactt ggtaagaaga aaaacgggag aaaccatgct tgcacagtta ttccgcacca
      481 aacccatcgt tccccatacc gatagtggcc tgaaaaagtg cctcactgcc ttcgacctgg
      541 ccttgctcgg catcggctgt gccatcggca ccggcatctt cgtgctgaca ggcatcgccg
      601 ccgcgaccca atccggcccg gcagtggtgt tatcgtttgt cttcgccggg atcgcctccg
      661 cctttgccgc gttctcgtat gccgagctgg cagcggcggt cggcggctcc ggtagcgctt
      721 atggttatag ctatgtggct tttggtgagt tcttcgcctg ggtgatcggc tggatcttgc
      781 tgctcgaata cggggtcggc gctgcagccg tcgccaatgg ctggtctgga tatttcatca
      841 acacgctggc gaatttcagc atccacatcc ccacgacctg gacacgggca ccaagcctgg
      901 gaggcgtcat caacctgccc gccttcgcca tcatctggct gctgaccttg ctgctgatcg
      961 cgggggtgaa ggaaagcgcg cgcttcaaca atatcatcgt cgtcgtcaag ctgagcacca
     1021 tcgtcatctt catcgcgctc gcctcaatgc acctgaatag cgacaactgg catccattca
     1081 tgccgttcgg ctggttcgat acggtcgaga acggcaagac gatcggcgta ctggcaggcg
     1141 cctcgctggt gttcttcgcc tatttcgggt tcgatgcggt ctccaccgct gccgacgaag
     1201 ccaagaatcc gcaacgcgac ctgccgatcg gtctcatcgc ttcgctcagt ttctgcacca
     1261 tcgtctatat cctggtttcg ggcgcgctga ccggtatcgt gccctacacc cagctcaatg
     1321 tggattctcc cgtggcttgg gcactgagtc agctcggcta cacctggtct tcgaccttgg
     1381 tggctactgg cgtactcgct ggcctcatca ctgtcttgct ggtactgctt tatggcctga
     1441 cgcgcatcct gttcgccatg agccgcgatg gcctgatctc gcccgtattc tccaaggtcg
     1501 acccggatcg tcagacaccg gtacgtatca tcctcatgtg cggctttgtc gtctcgctgg
     1561 tagctggttt cttcccgctt ggcgcgctgg cggagacggt caatatcggc acactggcca
     1621 gcttcgtcat ggtctgcgca ggcgtgatcg tgatgcgcat gcgtcacccg gacctgcatc
     1681 gcccattcag gaacccctgg aacccactga tcccggtact gggtatcctc tcctgtacgg
     1741 cgttgatgac attcctgcct gcgggcactt ggcatcgctt cctgttatgg gtcgcggcgg
     1801 ggatcgtctt ttatttcagc tat
//
LOCUS       DATMTW010000098         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_444290, whole genome shotgun sequence.
ACCESSION   DATMTW010000098 DATMTW010000000
VERSION     DATMTW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444290"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1884
                     /locus_tag="VN084_01870"
     CDS             175..1884
                     /locus_tag="VN084_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT28231.1"
                     /translation="MPNPISLRRAVVAAALTIVAASASAEDTKATPQSPATSAGMQAQ
                     LSSEFVYKYLVGEVAGQRGDPGLASSIFLDLAKSSRDPRLAERAAKAAVYGNKPKVAL
                     QATALWAELDPSSIEAQQATTQMLVSTGNLQEAKPHLQKLLAKEDTRANGFLYLNGLF
                     ANQQDKQAVLALVQELAKPYPELPEAHFTIAHTAWAAGNDDLAISELAQADKLRPGWE
                     TSAILHGQVLYRQSPDLAMGFYRDFLSHHANASEVRLTYARLLVNEKQFAAAKTEFIK
                     LVQASNNNPEVLVVVGLLSLQSMELADAEKYFLQAEKAGFKDPDQLYLYLGQIAERQK
                     KDDKALAMYDKVQPGEHYLDAQLNTAAVMARTQGTDAGIAQLDRLTDLQSEEQAIVAQ
                     TRANLLTQAKRYDEAYSVLENAVSTLPNSPELIYDYAMAAERVQKLDVMERELRKLIR
                     LKPDFAQAYNALGYTLADRNLKLEEARSLIEKALALSPNDHYILDSMGWVHYRLGKLD
                     AAADYLRRAYTAQTDPEIAAHLGEVLWKQGKYSEALQTWEEALKVHPENEVLINTTKK
                     FKQ"
     gene            1891..2517
                     /gene="lolB"
                     /locus_tag="VN084_01875"
     CDS             1891..2517
                     /gene="lolB"
                     /locus_tag="VN084_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HWT28232.1"
                     /translation="MTAIQRWHWLAVMLVLTGCAGLQPTPQRSASSPEVHQRHLASLA
                     DIQRFNIEGRIGVQTDGRGFSGATRWQHAEDRDKIGLFSPLGGQVANIERTRSGVVLT
                     DGDGKSYTAPDAETLTRQTMGWTLPMRGLPDWALGRPSPDAIELAVWDAEGKLLRLQQ
                     AGWDIRYQAYVQSGDHQLPSKILMVSPKVNLKLLIERWQLGGPAAAMP"
     gene            2517..>3358
                     /gene="ispE"
                     /locus_tag="VN084_01880"
     CDS             2517..>3358
                     /gene="ispE"
                     /locus_tag="VN084_01880"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWT28233.1"
                     /translation="MAGFQAFPAPAKVNLFLHITGRRADGYHLLQSVFRLLDRYDTIH
                     LKPNRTGLIGRVNDVPGVPEAHDLCIRAARLLQQHTGTRQGVEIAVDKHIPMGGGLGG
                     GSSDAATVLLALNRLWDLNLPRQTLMDLGLKLGADVPVFIFGRNAWAEGVGEVLQAVS
                     LDPAWYLVLTPDAHVSTAEIFASKELTRDTIPTTIAAFSGVTSGKEQEGLFHNDLEPV
                     VCQRYPAVASSLEFLKKFGQARMSGSGASVFVAFDQQAAAESSYAELPKHDVGFLAKG
                     LEQHP"
BASE COUNT          725 a         1093 c          946 g          594 t
ORIGIN      
        1 ctgctttgag acgctttccg tgcggaaagt tctacttggc aaagatctat gcaaaaagct
       61 atagtcagac ttaattatgg atgaacttgc atccggtgtc acggactaag ctatgactgg
      121 ataaccgccg cactatccat aaatccctac atctgtatcg agaacattct gaatatgcct
      181 aaccccatct ctctgcgtcg tgctgtcgtc gccgctgcgc tcactatcgt agccgcctca
      241 gctagcgccg aggacaccaa agccaccccg cagagcccgg caaccagcgc aggcatgcag
      301 gcccaactca gcagcgaatt cgtctacaaa tatctggtgg gcgaagttgc cggccagcgc
      361 ggcgaccccg ggctcgccag cagcatcttc ctcgatctcg ccaaatcgag ccgcgatccg
      421 cgtctcgcag agcgcgccgc caaggcggcc gtgtacggca acaaacccaa ggtggcgctg
      481 caggcgacgg ccttgtgggc tgagctcgac ccgagctcga tcgaggcaca acaagccacc
      541 acgcagatgc tggtctccac cggcaacctg caagaagcca aacctcatct gcaaaaactt
      601 ctggcgaagg aagacacccg tgccaacggc ttcctctacc tcaatggcct gttcgccaac
      661 cagcaggaca aacaggctgt actggccctg gtgcaggaac tggccaagcc atatcccgaa
      721 ttgccggaag ctcacttcac gatcgcgcac accgcctggg ctgcgggtaa cgacgatctc
      781 gcgatcagcg aactggccca ggccgataag ctgcgcccgg gctgggagac cagcgctatc
      841 ctgcacggcc aggtgctcta ccgtcagtcg ccagatctcg ccatgggctt ctatcgggat
      901 ttcctctccc atcacgccaa tgcgtctgaa gtccgcctga cctatgcgcg cctgctggtc
      961 aacgagaaac agttcgccgc ggccaagacc gaattcatca agctggtgca ggcctccaac
     1021 aacaaccctg aagtactggt cgtcgtcggc ttgctgtcac tgcaatcgat ggaactcgcc
     1081 gatgccgaga aatactttct gcaggcagaa aaagcaggat tcaaggatcc ggaccaactc
     1141 tatctctatc tcggccagat cgccgaacgc cagaagaagg atgacaaggc cctcgccatg
     1201 tacgacaagg tgcagccggg cgagcactat ctggacgcgc agctcaacac cgctgcggtc
     1261 atggcccgca cacagggcac ggacgcaggc atcgcccagc tcgaccgcct gaccgacctg
     1321 cagagcgaag agcaggcgat cgtcgcccag acccgcgcca acctgctgac gcaggccaaa
     1381 cgttatgacg aagcctacag cgtgcttgag aatgcggtaa gcacactgcc caacagcccg
     1441 gaactgatct acgactacgc catggcggcg gagcgcgtgc aaaaactgga cgtgatggag
     1501 cgcgaactgc gcaagctgat ccggctcaag cctgatttcg cccaagccta caacgcgctg
     1561 ggttacacgc tggctgatcg caacctcaag ctggaggaag cacgcagcct gatcgaaaaa
     1621 gccctggcct tgagcccgaa tgaccactat atcctcgaca gcatggggtg ggtccattac
     1681 cgtctgggca agctcgatgc cgccgcggat tacctgcgcc gcgcctacac tgcgcagacc
     1741 gaccccgaga tcgccgcgca cttgggcgaa gtgctctgga aacagggcaa gtattccgaa
     1801 gccctgcaga cctgggaaga agcgctcaag gtgcatcccg agaacgaagt gctgatcaac
     1861 accaccaaaa aattcaagca ataagccatg atgacagcca tccaacgctg gcattggctg
     1921 gctgtcatgc ttgtgctcac cggttgcgcc ggcttgcagc ctacaccgca acgctccgcc
     1981 agctcgcctg aagtccacca gcgtcacttg gcgagcctcg ccgatatcca gcgtttcaac
     2041 atcgaaggtc gcatcggcgt ccagaccgac gggcgcggct tctctggcgc gacgcgctgg
     2101 cagcatgccg aggatcgcga caagatcggg cttttctcgc cgctcggtgg ccaggtcgcc
     2161 aacatcgaac gtacccgcag cggcgtggtg ctgaccgatg gggacggcaa atcctataca
     2221 gcccctgacg ccgagacctt gacgcgacag accatgggct ggaccctgcc catgcgcggt
     2281 ttgccggatt gggcgctggg ccgccccagc ccggatgcga tcgaactcgc cgtctgggat
     2341 gcagaaggca aactgctgcg tctgcaacag gcaggctggg atatccgcta tcaggcctat
     2401 gtgcaatccg gcgaccacca gcttccgagc aagatcctga tggtgagccc caaggtcaac
     2461 ctcaagctgc tcatcgagcg ctggcaactc ggcggcccgg ccgcggcaat gccatgatgg
     2521 ctggtttcca ggccttcccc gcgccggcca aggtcaacct ctttctccac atcaccgggc
     2581 gccgtgccga tggttatcac cttctgcaat cggtgttccg tctgctggat cgctacgaca
     2641 ccatccacct caaacccaat cgcaccgggc tgattggacg cgtcaacgat gtgcccggcg
     2701 tgcccgaggc gcacgatctc tgcatccggg ccgcccgact actgcagcaa cacaccggta
     2761 cccggcaagg tgtcgagatc gctgtcgaca agcacatccc catgggcggc ggcctcggcg
     2821 gcggcagctc ggacgcggcg accgtcctgc tggcgctcaa ccgcttatgg gacctcaatc
     2881 tcccgcgcca gacactgatg gatctgggcc tcaagttggg cgccgatgtg cccgtgttca
     2941 ttttcggccg caacgcgtgg gccgaaggcg tcggcgaagt gttgcaggca gtctcgctgg
     3001 acccggcctg gtacctcgta ttgacgccgg acgcgcacgt ctcgaccgcc gaaatttttg
     3061 ccagcaagga attgacaagg gacacgattc ccaccacaat agcggccttt tctggggtca
     3121 cttccgggaa agaacaggaa ggcttgtttc acaacgatct cgaacccgtg gtctgccagc
     3181 gttatcctgc cgtggcgtcc agtcttgaat tcttgaaaaa attcggtcag gcacgcatga
     3241 gtggttctgg tgcatcggtg ttcgtggcgt tcgatcagca agccgcagcg gagtcgagct
     3301 atgccgagtt accgaaacat gatgtcgggt ttctcgccaa gggtctggaa cagcaccc
//
LOCUS       DATMTW010000099         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_545203, whole genome shotgun sequence.
ACCESSION   DATMTW010000099 DATMTW010000000
VERSION     DATMTW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545203"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..193
                     /locus_tag="VN084_01885"
     tRNA            118..193
                     /locus_tag="VN084_01885"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Gly,seq:gcc)
     gene            250..323
                     /locus_tag="VN084_01890"
     tRNA            250..323
                     /locus_tag="VN084_01890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:282..284,aa:Cys,seq:gca)
     gene            533..2659
                     /locus_tag="VN084_01895"
     CDS             533..2659
                     /locus_tag="VN084_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWT28234.1"
                     /translation="MQNDITDTLLECLLVVCRMNGVAISRDALIAGLPLKEGRLSPGL
                     VKRAAARANLVSNILKRPLSAIRGEFLPVILLLEEQDACLLLGWDDQRKFARVIFPEL
                     GDAEVRVPLEELAQRYSGYAIAAKPKFLFDQRAPSVGNVKLKHWFWGTLAENAKIYQD
                     IMLAAFLINLFALAMPLFTMNVYDRIVPNRSLETLWVLVVGIVVVISADFALRTMRAY
                     FLDWASHRVDIKLSGRIMEQVLGLKLEQRPNSVGSFAQNLRSFENVRDFITSATITTL
                     IDIPFALIFVLVIAWISWPMILPLLTGAVIMLIYALSVMTRLHEISETMFRASAIRNA
                     TLIESLVGLETVKALGIEGQMQRKWEKSAAFLTEVGGKMKLLSTSINNGAMGVQQVVN
                     VMMVLLGVYLVIEGELTMGGLIACSMLSGRALAPVSQAAGLMTQYHNSSTSLASLDDL
                     MNKPVERPIDSNFLSRKSFAGDIEFREVSFNYPGSEVNALKEVSFKIRAGEHVAILGR
                     MGSGKTTLHKLILGLYEPTEGAVMVDGIDLRQIDPAELRRSIGYVQQDTNLFYGTIRD
                     NIAVAAAHADDEQVLAAARMGGIDGFINAHPKGFDMLVGERGETLSGGQKQGVGIARA
                     MITNPAIVLLDEPTSAMDHSGEEAIKKRLIEATAGKTLMVITHRSSLFDLVDRIIVID
                     GGKIVADGAKDQVIEALRTGRVGKSV"
     gene            2656..4068
                     /locus_tag="VN084_01900"
     CDS             2656..4068
                     /locus_tag="VN084_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWT28235.1"
                     /translation="MSQDLFKRMGKLHDFFSRHRWITAPVHKLLDMFSSKHTPDDLEW
                     HEEAEIALEEQAPLRARRMLYWIGGVLLAIIIWAAFAEIDEVTRGEGRVIPSSQVQII
                     QSLDGGIVSEILVHEGETVSVGQPLIRIDETRAASSLRENRVQYLSLVAKQARLKALA
                     EDTTFNPPAEVKEENPTVYSQEASLYLASKEELASQIQIAREQKTQRERELVEAKAKL
                     DQVERGLELASRELTVTKPLEASGAVSEVDLLKLEREVSKYRGDREQTKAQISRLQSA
                     ILEADSKIDEISQNFRSKVRTDLTETTAKLNSLNESSVALSDKVAQATLKSPVNGTVS
                     RLFYNTVGGVIQPGKEVLEVVPTDDALILETKIQPKDIAFIKLLQPATVKLTAYDYTV
                     YGALDAIVENISADSVVDEKGNAFYIVRVRTLRSNLGKGLPIIPGMVAQVDIMTGKKT
                     VLSYLLKPVLKAKSYAFTER"
     gene            4068..4691
                     /locus_tag="VN084_01905"
     CDS             4068..4691
                     /locus_tag="VN084_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWT28236.1"
                     /translation="MRDLFICPRNVMRDNWAEACPKAKLYSDVGSIPKKGNDEAIVWI
                     HCDPANVAEVFASIGQVLSRLERCKVIMLSNTPDQAEAFQAMSAGVVGYCHALSPPRF
                     LREVRAVVAHGGIWLGAELLKSLMLASKPLVGNAPQTVADNLALLTKREREVALEAAK
                     GLSNKEIARVLDITERTVKAHLSASFERLGLKDRLQLALMLNERIHS"
     gene            4796..>5870
                     /locus_tag="VN084_01910"
     CDS             4796..>5870
                     /locus_tag="VN084_01910"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HWT28237.1"
                     /translation="MAQVGTVVAVNGIAYIIKPNGQQHQIKLGDTVDPGDTIVTTRGV
                     IVELELISGRVVQIASEESVKFTDELIFATDQTDPTEFAIDLATIQTIIKAIEEGRDV
                     SEVLSETTAGIDGGNLTNYGFSFVELSRISELLNNFTFDFDRAFSNFNPSTQTPLGLT
                     RPGDSAEFIQVGTGLVGDTPNSAPVASASAPAQGSEDGALLSGSLSASDADGDALTFR
                     LLSAAPPGMVFHTDGSWTLDPSDASYQSMDAGQVSHWVGTYQVSDGKGGTDTATFVID
                     VTGTNDIPVASAATDNATEDGAVVTGSVSATDADANANATLTYALDNTVTGLTLATDG
                     SYSFDPASYDALGAGQTTDLVATY"
BASE COUNT         1274 a         1708 c         1677 g         1211 t
ORIGIN      
        1 aggcgcatcg gcttgatgga tgaaaaaatt aatgtatact ctcgcctcgc tgatcggcta
       61 gccgccagaa ttctcaagat tcaaacggac cctcagcgat gcggtcagtg tgcaaatgcg
      121 ggagtagctc agttggtaga gcgcaacctt gccaaggttg aggtcgcgag ttcgagactc
      181 gtttcccgct ccagcattat aaaaacgggg aaggctgtcg ctttccccgt tttagtttca
      241 gcctcaaatg gcgcgatagc aaagcggtta tgccgcggcc tgcaaagccg tttaggccgg
      301 ttcgactccg gctcgcgcct ccactacttc cctgtcactt ctaaatctcc gtctctagcc
      361 atgtggcacg catcttgcca cttgcttcat atttcacgcc gaatcttgtg ccggacactg
      421 gcatggatcg ataaatcagc taaactgaca taaatcgatt cagtgcatga ctgttcacgc
      481 tgggcatggt tttggatatt gcatctaaac aataaagacg gtttttagaa gaatgcaaaa
      541 cgacatcacc gatacgttac tcgaatgcct gttagtggtt tgccgaatga atggggtcgc
      601 catctcccgc gatgccctga tcgccggcct gccgctcaag gaaggccggc tctcgccggg
      661 ccttgtcaag cgtgcagcgg cacgggccaa ccttgtcagt aatatcctga agagaccgct
      721 ttcggccatc cgtggcgaat tcctgccagt gatcctgctg ctggaagaac aggacgcctg
      781 cctgctactg ggctgggacg accagaggaa gttcgcgcgc gtgatcttcc cggaactggg
      841 tgacgccgaa gtacgggtgc cgctggaaga actggcgcaa cgctactccg gctacgcgat
      901 cgcagccaaa cccaaattcc tgttcgacca gcgcgcgcct tcggtcggta acgtcaagct
      961 caagcactgg ttctggggca cgctggctga gaacgcgaag atctaccagg acatcatgct
     1021 ggcggcgttc ctcatcaatc ttttcgcgct ggcgatgccg ctgttcacca tgaacgtgta
     1081 cgaccgtatc gtgcccaatc gttcactgga gactctgtgg gtgctagtgg tcggcatcgt
     1141 cgtcgtcatc agcgccgatt ttgctctgcg caccatgcgc gcctatttcc tcgattgggc
     1201 cagccaccga gtcgatatca agctttccgg ccgtatcatg gagcaggtgc tggggcttaa
     1261 gctcgaacag cggccgaatt ccgtcggctc gttcgcccag aatctgcgct ctttcgagaa
     1321 cgttcgagat ttcatcactt ccgccaccat cacgacattg atcgatatcc cgttcgcgct
     1381 catcttcgtg ctggtgatcg cctggatctc ctggcccatg atcctgccct tactgactgg
     1441 cgcggtcatc atgctgatct acgccctcag cgtcatgacg cgcctgcacg agatctcgga
     1501 aacgatgttc cgcgccagcg cgattcgcaa tgccacgctg atcgaaagcc tggtcgggct
     1561 ggaaaccgtc aaggcgctgg gtatcgaagg ccagatgcag cgcaagtggg agaagagtgc
     1621 cgccttcctc accgaggtcg gtggcaagat gaagctgctg tcgacctcca tcaacaacgg
     1681 cgcgatgggc gtgcagcaag tggtcaacgt catgatggtc ctgctaggcg tctatctcgt
     1741 catcgagggt gaactgacca tgggtggcct cattgcctgt tccatgcttt ccggtcgtgc
     1801 actggcgcca gtctctcagg ctgccggctt gatgactcaa taccataact cctcgacctc
     1861 gctcgcttcg ctagacgacc ttatgaacaa gccggtggaa cggccgatcg attcgaactt
     1921 cctctcccgt aaatcctttg ccggagatat cgagttccgt gaggtgagtt tcaattatcc
     1981 gggcagtgaa gtgaatgcgc tgaaggaggt gagcttcaag atccgcgccg gcgaacatgt
     2041 tgccatcctc ggtcgcatgg ggtcgggcaa gaccaccttg cataagctga tccttggcct
     2101 ctatgagccg acggaaggcg ccgtcatggt cgatggcatc gacctgcgcc agatcgaccc
     2161 cgccgaattg cggcgcagca tcggttacgt gcaacaggac accaatctat tctatggcac
     2221 gatccgcgac aatatcgccg tagcggcggc gcatgcggac gacgagcagg tgctcgcggc
     2281 tgcgcgcatg ggcggcatcg atggcttcat caatgcgcat ccgaaaggct ttgacatgct
     2341 ggtgggcgag cgtggcgaaa ccctgtccgg cggccagaag caaggcgtgg ggatcgctcg
     2401 ggcgatgatc accaacccgg ccatcgtctt gctggacgaa ccgaccagcg ccatggacca
     2461 ttcgggcgaa gaggccatca agaagcggct gatcgaagcc acggcaggca agaccctgat
     2521 ggtcatcaca catcgttcat cgctgttcga tctggtcgac aggatcatcg tcatcgacgg
     2581 cggcaagatc gtcgctgacg gcgccaagga tcaagtcatc gaggcgcttc gcacgggcag
     2641 agtagggaag tcggtatgag tcaggatctg ttcaagcgca tgggtaagct ccatgatttc
     2701 ttcagtcgcc atagatggat cacggcgccg gtccataaat tgctggatat gttcagttcc
     2761 aagcacacgc cggatgacct cgaatggcat gaggaagccg agatcgcgct cgaggagcag
     2821 gcgccactgc gcgcgcgccg catgctctac tggatcggcg gcgtgctgct ggccatcatc
     2881 atctgggccg cattcgccga gatcgatgaa gtgacgcgtg gcgaagggcg ggtcatccct
     2941 tccagtcagg tgcagatcat ccagtccctc gatggcggca tcgtatccga gatcctggtg
     3001 catgaaggcg agaccgtgtc tgtggggcaa ccgctgatcc gtatcgatga gacaagggcg
     3061 gcgtcatcct tgcgtgagaa tcgcgtccag tatctatcac tggtcgccaa gcaggcacgc
     3121 ttgaaggcat tggcagaaga taccactttc aaccccccgg ccgaggtcaa ggaagagaac
     3181 ccgaccgtct actcgcaaga ggcatcgctc taccttgcca gcaaggaaga gctggcgtcg
     3241 cagatccaga tcgcgcggga gcaaaagacc cagcgtgaaa gggagctggt cgaagccaaa
     3301 gccaagctcg atcaggttga gcgcgggctg gagctcgcca gtcgcgagct gacggtgacc
     3361 aagccgctgg aagccagtgg cgcagtgtcg gaagtcgatc tgctgaagct cgaacgtgag
     3421 gtctccaagt atcgcggcga ccgcgaacag accaaggcac agatcagtcg cttgcagtcg
     3481 gctatccttg aagcagacag caagatcgat gagatcagtc agaattttcg tagcaaggtg
     3541 cgaacggatc tcaccgagac cacggccaag ctcaatagcc tgaacgagtc cagcgtcgca
     3601 ctctcggaca aggtggcgca agcgacgctc aaatcccccg ttaacggcac cgtcagcagg
     3661 ttgttctata acactgtagg cggtgtgatc cagccgggta aagaggtact cgaagtggtg
     3721 ccgacagacg atgcgctgat cctcgaaaca aagatacaac ccaaggacat cgccttcatc
     3781 aagttgctgc aaccggcgac cgtcaagctc acggcttacg attacacggt ctatggtgcg
     3841 ctggatgcca tcgtcgagaa catctccgcc gactctgtcg tcgatgagaa aggcaatgcc
     3901 ttctacatcg tgcgggtgcg caccctgcgc tccaacctcg gcaaggggct accgatcatt
     3961 ccggggatgg tggcccaggt ggatatcatg acgggtaaga aaaccgtgct ttcctatctg
     4021 ctgaagcctg tgctgaaagc gaagtcttac gcctttaccg agagataatg cgcgatctat
     4081 tcatttgccc gcgcaatgtc atgcgggaca attgggcaga agcttgcccc aaagcgaagc
     4141 tctactccga cgtcggatcg atcccgaaaa agggtaacga cgaggctatc gtctggatcc
     4201 attgcgatcc ggccaacgtg gccgaagtct tcgcttccat tggtcaggta ctcagtcgcc
     4261 tcgagcgttg caaggtcatc atgctctcca acaccccgga tcaagccgag gcttttcaag
     4321 ccatgtccgc cggtgtggtt ggctattgtc acgccttgag cccacccagg ttcctcagag
     4381 aggtgcgtgc agtcgtcgcg catggcggga tctggctggg ggctgaactc ttgaagagcc
     4441 tcatgctggc gagcaagccg ctggtcggta acgccccgca gaccgtggcc gataacctcg
     4501 cgttgctcac caagcgcgaa cgcgaagttg cgctggaggc ggccaagggt ttgagcaaca
     4561 aggagatcgc acgggtgctc gatatcactg agcgcaccgt caaggcgcat ctttcggcga
     4621 gtttcgagcg cctggggctg aaggatcggc tacaacttgc attgatgctg aacgaaagaa
     4681 tccacagctg aaatacgcct aatattgtcc ttcaggacaa tatatttttg tcgacacatc
     4741 ttctttaata gctgcatcgg taaaagtctc cagatcacct ggaaagaggc atcacatggc
     4801 acaagtcgga accgtcgttg cagtcaatgg catcgcttac atcatcaagc cgaatggcca
     4861 gcagcatcag atcaagctgg gggataccgt cgatccgggc gataccatcg tcaccactcg
     4921 cggcgtgatc gttgagctgg agctcatcag tggtcgtgtc gtacagatcg catccgaaga
     4981 atcggtcaaa ttcactgatg agctgatctt tgccacggac caaaccgacc ccaccgaatt
     5041 cgccatcgac cttgccacga tccagaccat catcaaagct atcgaagagg ggcgcgacgt
     5101 cagcgaggtg ttgtctgaga cgaccgccgg tatcgatggc ggcaatctga cgaattacgg
     5161 tttttctttc gtcgaactgt cgcgcatcag cgagctgctc aataacttca cattcgattt
     5221 cgatcgtgcg ttcagtaatt tcaatccatc aacacaaaca ccgcttggcc tgacccgtcc
     5281 gggggatagc gccgaattca tccaggtcgg cacgggtctc gttggcgata cgccaaattc
     5341 ggcacctgtt gcctccgctt cagccccggc gcaaggatcc gaggatggcg ccttgctctc
     5401 ggggagtctg tcggcaagcg atgcggacgg ggatgcgctt actttcaggt tgttgtctgc
     5461 tgcgcctcct ggcatggtgt tccacactga tggcagttgg acgctggatc cttctgacgc
     5521 cagctatcag agtatggacg ctggtcaagt ctcgcattgg gtcggcactt atcaagtgag
     5581 cgacggtaaa ggcggaacag ataccgcgac attcgtcatc gatgtcaccg gcaccaatga
     5641 cattccggtc gccagcgccg ccactgacaa cgccaccgaa gacggtgctg tcgtcaccgg
     5701 cagcgtcagt gccaccgacg ccgacgccaa cgccaacgcc acgctgacgt acgccctcga
     5761 caacacggtc accggtctca cgctcgccac cgacggcagc tacagcttcg accctgcgag
     5821 ctatgacgcc ctcggtgccg gccagaccac ggacctcgtc gccacctaca
//
LOCUS       DATMTW010000100         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_142023, whole genome shotgun sequence.
ACCESSION   DATMTW010000100 DATMTW010000000
VERSION     DATMTW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142023"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2282
                     /gene="fdhF"
                     /locus_tag="VN084_01915"
     CDS             <1..2282
                     /gene="fdhF"
                     /locus_tag="VN084_01915"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWT28238.1"
                     /translation="GFESKVAAGVHDFMDSECVSCGACVQACPTATLMENSVIQHGIP
                     EHSVTTTCAYCGVGCSFNAEMKGEEVIRMMPNKDGGANHGHSCVKGRFAWGYATHKDR
                     ITTPMIRKSIKDPWQEVSWEEAIGYAASEIKRIIGQYGKDAVGAITSSRCTNEEVYVV
                     QKLVRAVFGTNHVDTCARVCHSPTGYGLKQTLGESAGTQTFDSVMHSDVIMIIGANPT
                     DGHPVFASQMKRRLREGAKLIIADPRAIDLVSSPHIKADHHLKLRPGTNVALISALGH
                     VVVTEGLAKEDYIRERCEWDSYVIWRDFMAKPENSPEALEAELGIPAAEVRAAARLYA
                     TGGNAAIYYGLGVTEHSQGSTMVMGIANLAMATGNLGREGVGVNPLRGQNNVQGSCDM
                     GSMPHEFTGYRHVSDDATRALFEAAWGRPLSKDPGLRIPNMLDLAGEGLFKAVYCNGE
                     DIAQSDPNTQHVTHALESMECVIVQDLFLNETAMYAHVFFPGASFLEKSGTFTNAERR
                     ISPVRRVMTPKNGMEDWEITARLSEALGYPMHYNHASEIMDEIADLTPTFHGVSFKKL
                     DELGSIQWPCNEEHPVGTPIMHVDEFVRGKGKFYITQYIPTNERVTPKYPLILTTGRI
                     LTQYNVGAQTRRTENVHWHHEDLIEIHPHDAEERGIREGDWVGIASRAGNTVLRATIT
                     ERVQPGVVYTTFHHPESGANVITTDNSDWATNCPEYKVTAVQVTRVSQQSEWQEQYRQ
                     FSREQIALASLQPDEERVG"
     gene            2286..3119
                     /gene="fdhD"
                     /locus_tag="VN084_01920"
     CDS             2286..3119
                     /gene="fdhD"
                     /locus_tag="VN084_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWT28239.1"
                     /translation="MKLPADLIGTEATSSEYSVVKWEAGQRIAHDDVVAEEVPVALIY
                     NGVSHVVMLATPQDLEDFAVGFSLSEGIITRADEIYGVEIQPQPQGIELHIALAADRF
                     FMLKERRRNLTGRTGCGLCGAESLAQALRIPESLGTTGHFDHAAIVAAHQNIRARQPL
                     QAVTGATHASAWVRPDGEIALVREDVGRHNALDKLIGARARSRAQGEGFVLTTSRASY
                     EMVQKTASAGIPMLVAVSAPTGLAIRIARNCGLTLVGFARQQQYVIYSHPERILHKEY
                     A"
     gene            3116..3340
                     /locus_tag="VN084_01925"
     CDS             3116..3340
                     /locus_tag="VN084_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HWT28240.1"
                     /translation="MSIERLITMANQIGEFFRANPDSAQAKEDIVNHLKKFWAYNMRQ
                     QILVHVQEKQGAGLEPIVGEAIREHQASLL"
BASE COUNT          729 a         1047 c         1020 g          643 t
ORIGIN      
        1 gtggcttcga atccaaggtc gccgccggcg tccacgattt catggattcc gaatgcgtct
       61 cctgtggcgc ttgcgtacag gcttgtccga ctgcgacctt gatggagaac tccgtcatcc
      121 agcacggcat cccagaacac agcgtcacca ccacctgtgc ctattgcggc gtcggctgtt
      181 cgttcaacgc cgagatgaag ggcgaggaag tcatccgcat gatgccgaac aaggacggtg
      241 gcgccaatca cggccattcc tgcgtcaagg gtcgattcgc ctggggttac gccacgcaca
      301 aggatcgcat caccacgccg atgatccgca agagcatcaa agacccgtgg caggaagtct
      361 cgtgggaaga ggccatcggc tacgcggcca gcgagatcaa gcgcatcatc ggtcaatacg
      421 gtaaggacgc ggtcggcgcc atcacctcgt cgcgctgcac caatgaagag gtgtatgtcg
      481 tgcagaaact ggtgcgcgcc gtattcggca ccaaccatgt ggacacctgc gcacgcgtct
      541 gtcattcgcc gacgggttat ggcctgaagc agaccctagg ggaatcggcc ggcacgcaga
      601 ccttcgattc ggtcatgcat tccgacgtga ttatgatcat cggcgccaac ccgacggatg
      661 gtcatccggt gttcgcttcg cagatgaagc gccgcctgcg cgagggtgca aagctcatca
      721 tcgcggatcc gcgtgccatc gatctcgtct cttcgccaca tatcaaggcc gaccaccacc
      781 tcaagctgcg gccaggtacc aacgtcgcgc tgatctcggc cctgggccac gttgtggtca
      841 ccgaagggct ggccaaggaa gattacatcc gtgaacgctg cgaatgggat tcttatgtca
      901 tctggcgcga tttcatggcc aagccggaga actcgccaga agcgctggaa gccgaactcg
      961 gcatccctgc ggctgaagtg cgcgccgccg ccagactcta cgccacgggc ggtaatgcag
     1021 ccatctatta cggcctgggc gttaccgaac acagccaggg ctcgaccatg gtcatgggta
     1081 tcgccaacct cgccatggcg accggcaacc tcggccgcga aggtgtgggc gtcaatccgt
     1141 tacgcggtca gaacaacgtg caaggttcct gtgacatggg ctccatgccg catgaattca
     1201 cgggttaccg ccatgtctcc gatgacgcta cacgcgcctt gttcgaagct gcctggggtc
     1261 gtccgctgtc caaggatccg ggcctgcgca tccccaatat gctggatctg gccggtgaag
     1321 ggctgttcaa ggcggtttac tgcaatggcg aggatatcgc gcagtctgac cccaacactc
     1381 agcatgtcac gcacgcgctg gagtcgatgg aatgcgtcat cgtgcaggac ctcttcctca
     1441 atgagacggc gatgtatgcc cacgtgttct tcccgggcgc ttccttcctg gaaaagagcg
     1501 gcacattcac caatgccgag cggcgcatct cgccggttcg gcgtgtcatg accccgaaga
     1561 acggcatgga agactgggag atcaccgcca gattgtccga ggcgctgggc tacccgatgc
     1621 attacaacca tgcttccgag atcatggacg agatcgcaga tctgacgccg accttccatg
     1681 gcgtcagctt caaaaagctg gacgaactcg gcagtatcca gtggccttgc aacgaggagc
     1741 atccggtcgg cacgccgatc atgcatgtgg acgaattcgt gcgcggcaag ggcaagttct
     1801 acatcacgca atacatcccg accaacgagc gtgtgacgcc gaaatatccg ctgatcctga
     1861 cgacaggccg catcctcacc caatacaacg tgggtgcgca gacgcgacgt acggagaacg
     1921 tacattggca ccatgaggat ctgatcgaga tccatccgca cgatgccgag gaacgcggca
     1981 tccgcgaggg agattgggtg ggcatcgcca gccgggcggg taataccgtg ctgcgcgcca
     2041 cgatcaccga gcgggtgcag cccggggtgg tctacaccac gttccaccat ccggaatctg
     2101 gcgcgaatgt catcaccacc gataactcag actgggccac caactgcccg gagtacaagg
     2161 tgacggcagt gcaggtgacg cgcgtctcgc aacagtcaga atggcaggag cagtaccgtc
     2221 agttcagccg tgagcagatc gcgctcgcca gcctgcagcc ggatgaggag cgggttggct
     2281 aagtcgtgaa gctcccggca gacctgatcg gcaccgaagc caccagtagc gagtattccg
     2341 tcgtcaaatg ggaggcaggc cagcgcatag cccacgatga cgtcgttgct gaagaagtgc
     2401 ctgtcgcact gatctacaac ggtgtgtcgc atgtagtgat gctggccaca ccgcaagatc
     2461 tggaggactt cgcagtcgga ttctccttgt ccgaaggcat catcacgcgt gctgacgaga
     2521 tctacggtgt cgagatccag cctcagccgc agggcatcga gttacatatc gcccttgccg
     2581 ccgatcgctt cttcatgctc aaggaacgcc gccgtaacct caccggccgc accggatgcg
     2641 ggttgtgtgg tgccgagagt ctggcgcagg ctttgcgcat ccccgaaagc cttggtacca
     2701 cgggtcattt cgaccacgcc gcgatcgtcg ctgcgcatca gaacatccgc gcccggcaac
     2761 cattgcaggc ggtgaccggc gccacgcatg caagcgcctg ggtccggcct gatggcgaga
     2821 tcgcactggt acgcgaggat gtcggtcggc ataacgcgct cgataagttg atcggtgccc
     2881 gcgcgcgcag ccgcgcccag ggcgaaggct ttgtgcttac caccagtcgt gccagttacg
     2941 agatggtgca gaagacggcg tccgcgggta ttcccatgct agtcgcagtt tctgcgccta
     3001 ccggcctcgc gatccgcatc gctcgcaatt gcgggttgac cctggtcggt tttgcccgtc
     3061 aacagcaata cgtgatttat agccacccag aacgaatctt gcacaaggaa tacgcatgag
     3121 catcgaacgc ctcatcacca tggccaacca gatcggtgaa tttttccggg ccaaccccga
     3181 tagcgcgcag gcaaaagagg atatcgttaa tcacctgaag aaattctggg cgtataacat
     3241 gcgccagcag attcttgtgc atgtgcagga gaagcagggt gcaggcctgg agcctatcgt
     3301 tggcgaagcc atccgcgaac atcaggcgag cctgctttag cgtccccgat accagtttcc
     3361 ccaataaccg cccagcccga taaaaaagcc agcgatcgct ggctttttta tcgggctggg
     3421 cggttattgg ggaaactgg
//
LOCUS       DATMTW010000101         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_511767, whole genome shotgun sequence.
ACCESSION   DATMTW010000101 DATMTW010000000
VERSION     DATMTW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511767"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..899
                     /gene="ygiD"
                     /locus_tag="VN084_01930"
     CDS             132..899
                     /gene="ygiD"
                     /locus_tag="VN084_01930"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HWT28241.1"
                     /translation="MPAIFFGHGNPMNALEDNPYTRSWRAIGQSIPRPRAILCISAHW
                     YLAGTAVTANLAPPTIHDFGGFPDALYECRYPAAGSAELVDEVRETLAFIPPALEHGW
                     GLDHGTWSVLCHVFPEADVPVVQLSIDRTRPPQFHYDLGRKLASLRDQGILIMGSGNL
                     VHNLHAYAWGKPDAQPFDWAQRFETQARQLMADSDHDALIHYDTLGSDAMLSIPTPEH
                     YLPLLYILGAQQPGDQLSFPVEGIEGGSVSMLTVQVG"
     gene            902..1318
                     /locus_tag="VN084_01935"
     CDS             902..1318
                     /locus_tag="VN084_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28242.1"
                     /translation="MALRHDRTRFILLGLPCLLNIPALALFALNATTSGRGSCAFGLS
                     LILMAVCLVLSVPFILKRGRDLGQPAKGTFAFWLLGYAFPPLWLVLLIIYSIKPAAPA
                     ADQLGPAPARPGAGIIPLAILAYGWPWMAMLVWCRW"
     gene            1388..2131
                     /locus_tag="VN084_01940"
     CDS             1388..2131
                     /locus_tag="VN084_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWT28243.1"
                     /translation="MFEWIASPEAWMALATLTALEIVLGIDNIIFISILVGRLPEKQR
                     AFARRMGLSLAMLARLALLFSISWVMGLTEPWFELLGKAVSGRDFILIGGGLFLLAKA
                     THEIHNSLEGTEESEQSTASATLGIVLGQIAVLVIVFSLDSVITAVGLADHVSIMAIA
                     IVLSVGVMLLAAKAIGEFVDTHPTIKILALAFLILVGVTLMIEGFGIHVPKGYIYFAM
                     AFSVAVEMLNIRMRKKRAEAIRLRKALTK"
     gene            2138..2794
                     /locus_tag="VN084_01945"
     CDS             2138..2794
                     /locus_tag="VN084_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWT28244.1"
                     /translation="MQQILVYGDSLSWGIVPGTRQRLEFAQRWPGVMEIALRAAGHDV
                     RVIEDCLNGRRTVWDDPFKPGRNGLQGLAQRIEIHSPLTLVMLVLGTNDFQCMHDNTP
                     FLSAQGLAALINAIRQAPLEPGMAIPPILVVAPPAIVQPKGPIAGKFEGASLKSAGLA
                     AAYAELAQQLDCAFFDANSVTPASTVDGIHLDADQHMRLGQALADWFGSWSVRTKPHA
                     "
     gene            2880..3344
                     /locus_tag="VN084_01950"
     CDS             2880..3344
                     /locus_tag="VN084_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HWT28245.1"
                     /translation="MNGRLAILIDFGECRFYNKESSIMLIKRLFSLFVAASFLFVGNI
                     QIAQATLVSSEQVAQSAGIANAEQDRQRIYAALAREDVRSALVERGVDPKQVEQRVAA
                     LTDEEASVMADQIENAPAGSGIIGAIVLIFLVLLLTDILGFTKVFPFTRSVR"
     gene            3360..4322
                     /locus_tag="VN084_01955"
     CDS             3360..4322
                     /locus_tag="VN084_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HWT28246.1"
                     /translation="MSLQRHLLACALLLAAAVLSGCAAPGVREATQDSKLPRQHELAD
                     TPFFPQEQYQCGPAALATALNAIDIKVTPDQLTPEVYIPSRQGSLQIEMLAATRRHGA
                     IPYVIAPNLQALLREIAAGNNVLVMQNLGLSFAPSWHYAVVVGYDLDRELIWLRSGTY
                     QRFEMTLSTFERTWARSQHWAFVAKPPGVLPASIDAAQAARALIAFEQKAPAAAAEHA
                     YQAAVQRWPDDLVLLMGAGNSAYRNGDIAGAHHAFQSAAEQHPQSAAAHNNLASILME
                     QGKLDQAQQHASQALALAEGDAVLKQQVQQTLNEIDAKRHPAQK"
     gene            4433..>5149
                     /locus_tag="VN084_01960"
     CDS             4433..>5149
                     /locus_tag="VN084_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWT28247.1"
                     /translation="MPYYRTPLILSLLLTLAACGDVAQLPVSAGIGPDPTLPPPKKML
                     IPTVKTSPAVGWPTNARPSTASDLQVQAFASDLSHPRWVYRLPNGDILVAESSAPPKG
                     DSGFSLKGWISGIVMKNVGARVASPNRITLLRDADGDGVAEFRSTLLQGLHSPFGMAL
                     VGDQLYVANADAVLRFTYAAGQTEIKTPGEKLVDLPGLPLNHHWTKNIIASQDGSKLY
                     AAVGSNSNVGENGMDIEQGRA"
BASE COUNT          967 a         1712 c         1478 g          992 t
ORIGIN      
        1 gttcggacta acccgaatga cagcgtgcag tttgcgggta atataagcgg tgcaggatga
       61 gcgatagtgc tcccaacctt gaccaagcga cttgtggaga ccagaccaga gcgatgccca
      121 agacaacccc catgccagcc atcttcttcg gtcacggcaa ccccatgaat gcgctggagg
      181 ataatccgta tacccgcagc tggcgcgcga tcggccaatc gatcccacgg ccgcgcgcca
      241 tcctctgcat ctccgcccac tggtacctgg cgggtaccgc ggtcactgca aatctcgcac
      301 cgccgaccat ccatgatttc ggcggattcc cggatgcatt gtacgaatgc cgctaccccg
      361 ctgcaggcag tgcggaactg gtggacgagg tgcgggagac actggccttc atcccgccgg
      421 cgctggagca tggctggggg ttggatcatg gcacctggtc ggtgctctgc catgtgtttc
      481 cggaagccga cgtgccggtg gtgcagctca gcatcgaccg cacgcggccg cctcaattcc
      541 attacgacct tggccgcaag ctggcatccc tgcgtgacca gggtatcctc atcatgggca
      601 gcggcaatct ggtgcacaac ctgcatgcct acgcctgggg caaacccgac gctcaaccgt
      661 tcgattgggc acaacgcttc gagacgcagg cgcggcaatt gatggctgac agtgaccacg
      721 acgccttgat ccactacgac acgctgggca gcgatgccat gctgtccatc cccacgccgg
      781 aacactacct gcccctgctg tatatcctcg gcgcacagca accgggcgat cagctcagct
      841 ttcccgtcga gggcatcgaa ggcggctctg tctccatgct gacggtacag gtcggctgac
      901 catggccctg cgccacgacc gtacccgctt cattctgctc ggcctgccgt gcctgctcaa
      961 catcccggcg ctggcgttgt tcgcgctcaa cgccaccacc agcggcaggg gcagctgcgc
     1021 ttttggcctg tcgctgattc tcatggcagt ctgcctggtc ttgtccgtgc cgttcattct
     1081 caagcgcggt cgcgatctcg gtcagccggc caaaggtacg ttcgcgttct ggctactggg
     1141 ctatgcattc ccaccgcttt ggctggtttt gctcatcatc tacagcatca aacccgccgc
     1201 acctgcagcc gaccagctcg gcccggcacc cgcccggcca ggcgccggca tcatcccttt
     1261 ggctatcctg gcctatggct ggccctggat ggcgatgctc gtctggtgcc gatggtaatc
     1321 gtcaaccaca acggcggtct gtcttataat ccggaccacc agccttttca tcccacagga
     1381 gcccccgatg tttgaatgga tcgccagccc ggaagcctgg atggccctcg ccacactgac
     1441 cgcactcgag atcgtgctcg gtatcgacaa catcatcttc atctccatcc tggtcggccg
     1501 cctgccggaa aagcagcgcg ctttcgcccg caggatgggg cttagtctgg cgatgctggc
     1561 gcgtcttgcc ttgctgttct ccatctcctg ggtcatgggc ctgacggagc cctggttcga
     1621 gctgctgggc aaagccgtct ccggccgcga tttcatcctc atcggcggtg gccttttcct
     1681 gctggccaag gcgacgcatg agatccacaa cagcctggaa ggcactgaag agagcgagca
     1741 gtcaacggca agcgccaccc tgggcatagt gctggggcag atcgccgtgc tcgtcatcgt
     1801 gttctcgctc gattccgtca tcaccgctgt cggcctcgcc gaccatgtct ccatcatggc
     1861 gatcgcgatc gtgctgtcgg tcggcgtcat gctgctggcg gccaaggcca tcggcgagtt
     1921 cgtcgatact caccccacca tcaagatcct ggcactggcg ttcctgatcc tggtcggggt
     1981 cacgctgatg atcgaaggtt tcggtatcca tgtgcccaag ggctatatct acttcgccat
     2041 ggcgttctcg gtcgcggtcg agatgctcaa tatccggatg cgcaagaaac gcgccgaagc
     2101 catccggttg cgcaaggcat tgacgaaata gccggtcatg caacagatcc tcgtctatgg
     2161 cgactcactg tcctggggca tcgtcccggg tacgcgccaa cgtctggaat tcgcgcagcg
     2221 ctggcccggc gtcatggaga tcgcactacg cgcagcaggg catgacgtac gcgtcatcga
     2281 agattgcctc aacggccgcc gcactgtctg ggatgatccg ttcaagcccg gccgcaacgg
     2341 cctgcaagga ttggcgcagc gcatcgagat ccactcgccg ctgacgctgg tgatgctcgt
     2401 gctcggtacc aatgactttc agtgcatgca cgacaacacg cctttccttt ccgcgcaagg
     2461 gctggcggca ctgatcaacg ccatccgcca ggcgccgctg gagccgggca tggccatccc
     2521 gcccatcctc gtcgtcgcgc cgcctgccat cgtgcagccc aaaggaccga tcgccggcaa
     2581 gttcgaaggc gccagcctga agagcgcagg gcttgctgcc gcgtatgccg aactggcgca
     2641 gcaactcgat tgcgcgttct tcgatgccaa tagcgtgacg ccggccagca cggtcgacgg
     2701 tatccacctc gacgcagacc agcacatgcg cctgggtcag gcccttgcgg attggttcgg
     2761 ctcttggtcc gttcggacta agcctcacgc atagccagtc tctggcgttt tgtggtactt
     2821 ttacatcgct ggcggatcat aacgaggcgc atgccagcca cgaataagcg ccaacaggaa
     2881 tgaacggccg tctggccatc ttgatcgact tcggggaatg ccgtttctat aacaaggagt
     2941 cttccatcat gctcatcaaa cgtctgttca gtcttttcgt cgccgcatcc ttcctcttcg
     3001 tcggcaatat ccagatcgcg caggccactt tggtcagctc cgaacaggtc gcgcagtccg
     3061 ccggcatcgc caatgccgag caggaccgcc aacgcatcta tgccgcattg gcgcgcgagg
     3121 atgtccgcag cgcactggtc gaacgtggcg tcgaccccaa acaggtcgag cagcgcgtcg
     3181 ctgcgttgac cgatgaagaa gccagtgtga tggccgacca gatcgagaat gcccctgctg
     3241 gtagcggcat catcggcgcc atcgtactga tcttcctggt actgttgctg accgatatcc
     3301 tcggcttcac caaagtcttc cccttcaccc gctccgttcg ctgatcaggc aaggccggtt
     3361 tgtcgctgca aagacactta ctggcctgcg ccctgttgct cgcagccgcc gtactcagcg
     3421 gctgcgcggc accgggggtg cgtgaagcga ctcaggacag caagctgcca cgccagcatg
     3481 agctggctga cacgcccttc ttcccgcagg aacagtatca gtgcggtcct gccgcactcg
     3541 ccactgcgct caatgccatc gatatcaagg tgacgccgga tcaattgaca ccagaagtct
     3601 atatcccgag tcgccagggt agcctgcaga tcgagatgct cgcggccaca cgtcgccacg
     3661 gggccatccc ctatgtcatt gcacccaacc tgcaggcatt gctgcgggag atcgccgcag
     3721 gcaacaacgt gctggtcatg cagaacctgg ggctatcctt tgcgccttcg tggcactacg
     3781 ccgtggtcgt cggttatgac ctcgaccgcg aactgatctg gttgcgctcg gggacctacc
     3841 aacgtttcga gatgaccttg agcaccttcg agcgcacctg ggcacgtagc cagcactggg
     3901 ctttcgtggc caagccaccg ggtgtgctgc cggccagcat cgatgctgca caagctgcac
     3961 gggcgctcat cgccttcgag cagaaggccc ccgccgctgc cgctgaacac gcttatcagg
     4021 ctgcagtcca gcgctggccg gatgacctcg tgctactcat gggagcaggt aacagcgcct
     4081 atcgaaacgg cgatattgcc ggtgcgcatc atgctttcca gtcagcggcg gagcagcatc
     4141 cgcaatctgc cgccgcgcac aacaatctcg ccagcatcct catggaacaa ggcaagctcg
     4201 accaggcaca acagcatgcg tcacaggcac tcgcgctcgc cgagggcgat gccgtattga
     4261 agcaacaggt gcagcagacg ctgaacgaga tcgacgccaa gcgtcatcct gcacagaagt
     4321 agaccagatc aggcgctagc gcccttcgga aagaccatgc cagaggcgca cctcggtgca
     4381 cgcccatgtg acctgccgga gcgcatcgcc agccgtatga aaggaaccag gaatgccgta
     4441 ttaccgtacc ccactcatcc tctcgctgtt gctgaccctt gccgcctgcg gcgatgtcgc
     4501 ccagctaccg gtctctgccg gcatcgggcc cgacccgacc ttgccgccgc cgaagaagat
     4561 gctgatcccc accgtcaaga cctcgccagc cgtaggctgg ccgacgaatg cgaggccgag
     4621 taccgccagc gatctccaag tacaggcttt cgccagcgac ctgtcgcatc cgcgctgggt
     4681 ctacaggctg cccaatggcg atatcctcgt tgccgagagc agcgcaccgc ccaagggcga
     4741 cagtggattc agcctcaagg gctggatctc cggcatcgtc atgaagaatg tcggtgcccg
     4801 tgtcgccagc cccaaccgca tcactttgtt gcgtgatgcc gacggtgatg gcgtggcgga
     4861 attccgctcc acgctgctgc aaggcttgca ttccccgttc ggcatggcac tggtcggcga
     4921 tcagctatat gtcgccaacg ccgatgcggt gttgcgcttc acctatgccg caggccagac
     4981 cgagatcaag acacccggtg aaaagctagt cgacttaccg ggcctgccgc tcaaccatca
     5041 ctggaccaag aacatcatcg ccagccagga cggcagcaag ctctacgctg ccgttggctc
     5101 caacagcaac gtgggcgaga acggcatgga cattgagcaa gggcgagcg
//
LOCUS       DATMTW010000102         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_176588, whole genome shotgun sequence.
ACCESSION   DATMTW010000102 DATMTW010000000
VERSION     DATMTW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176588"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VN084_01965"
     CDS             <1..680
                     /locus_tag="VN084_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWT28248.1"
                     /translation="QSGDQQQVYLANGDGWVASFHLRETLRANAAQTVRALQSAGKQV
                     MLVSGDKRAAVEQVAQTLGITQVASECTPAEKLARLRALQLAGHQVMMVGDGLNDGPV
                     LAGARVSVAIGQRVPLAQRQSDIVLPHARLGELCTLFSQSTRTMRVVRQNLTWAFVYN
                     AVCVPLAVVGYLPAWLAGLGMATSSLLVVLNAARLSVIAPVAETQAAEAPGTKLEERA
                     AGARMAA"
     gene            680..817
                     /gene="ccoS"
                     /locus_tag="VN084_01970"
     CDS             680..817
                     /gene="ccoS"
                     /locus_tag="VN084_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HWT28249.1"
                     /translation="MEILFLLIPLSVVLVLAILAGLWWAIHTGQFEDVEKEGARILHE
                     D"
     gene            922..2358
                     /gene="ccoN"
                     /locus_tag="VN084_01975"
     CDS             922..2358
                     /gene="ccoN"
                     /locus_tag="VN084_01975"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWT28250.1"
                     /translation="MVSNNKTAAQYNDTALRQFSIMAVVWGVVGMLMGVIIAAQLVWP
                     ELNLGLPWTSFGRLRPLHTNAVIFAFGGSTLFATSYYVVQRTCQTTLFCPRLASFTFW
                     GWQAVIVAAAISLPLGFTSGKEYAELEWPIDILIAVVWVSYAIVFFGTIGRRKVRHIY
                     VANWFFGAFIIAVALLHIVNSAEMPASFMKSYSAYAGVQDAMVQWWYGHNAVGFFLTA
                     GFLGIMYYFVPKQAERPVYSYSLSIVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMV
                     FSLILLAPSWGGMINGIMTLSGAWHKLRTDPILRFLIVSLSFYGMSTFEGPMMAVKTV
                     NALSHYTDWTVGHVHSGALGWVGLVSMGALYYMVPRLFGQKQMHSIKAIEVHFWMATI
                     GIVLYISALWISGVMEGLMWRAINPDGTLTYTFVESVKAKHPYYLLRLLGGLLYLGGM
                     LVMLWNVLKTASKGQAVVTDIPPVAAHA"
     gene            2368..2988
                     /gene="ccoO"
                     /locus_tag="VN084_01980"
     CDS             2368..2988
                     /gene="ccoO"
                     /locus_tag="VN084_01980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HWT28251.1"
                     /translation="MTQEKTGFTHEKIETSNFLMIVLILLVVAIGGLVEIVPLFFQKS
                     TTEPIKGVEPYTALQLAGRDIYVREGCYNCHSQMIRPFRAETLRYGHYSVAGEFVYDH
                     PFQWGSKRTGPDLARVGGRYSDDWHRIHLTNPRDVVPESIMPEYPWLEKTLVDAEAMP
                     SHMRALRVVGVPYTDAQIAGSVDEVKGKTEMDAMIAYLQMLGTHLK"
     gene            2998..3135
                     /locus_tag="VN084_01985"
     CDS             2998..3135
                     /locus_tag="VN084_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HWT28252.1"
                     /translation="MDINTLRILVTVVSFVLFVGIVIWAWRGRNTQDFRQAANLPFEE
                     E"
     gene            3145..4182
                     /gene="ccoP"
                     /locus_tag="VN084_01990"
     CDS             3145..4182
                     /gene="ccoP"
                     /locus_tag="VN084_01990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HWT28253.1"
                     /translation="MSDFVSNFWPVFITLVTLAGIYGCGLLLWKTGKVKVIKGGDGTS
                     GHVWDGDLREMNNPLPKWWVGLFVITIVFSLVYLAAYPGLGKYEGQLGWTQVRQYEEE
                     VKAADQSLAPLYARFAAMDVAALSQDTEAHAIGERLFMNNCAQCHGSDARGSRGFPNL
                     TDHDWLHGGTPEIIKQTISAGRIGMMPPMAAAVGNDEDVKNVANYVLSLSGSAHDAQR
                     AALGKDKFVVCAACHGAEGKGNTMIGAPNLSDHIWLHGSGEEAIIRRIREGKTNQMPA
                     WSGKFTEAQIHVLTAYVWGLSNQGAPVGGDDRDAAGEKDSGEKDSGEKDSGEKNSTED
                     KPAVADGHDSD"
BASE COUNT          808 a         1325 c         1349 g          825 t
ORIGIN      
        1 cgcaatccgg cgatcagcag caggtgtatc tggccaatgg tgatggctgg gtggcgagct
       61 tccatctgcg cgagaccctg cgcgccaatg ctgcgcaaac cgtgcgcgcg ttgcagtcgg
      121 ctggcaagca ggtgatgttg gtttcgggcg acaagcgggc tgccgtagaa caggtggcgc
      181 agacgctagg gatcacgcaa gtggcgtcgg aatgcacgcc agccgagaaa ctcgcccggc
      241 tacgtgcact gcaattggcc ggacatcagg tgatgatggt cggcgatggc cttaacgacg
      301 ggccagtgct ggctggcgca cgggtctcgg tcgccatcgg ccagcgtgtg ccgctggccc
      361 agcgccagag cgatatcgtg ctgccgcatg cccggctcgg ggagttgtgc acgctgttca
      421 gccaatcgac acgcaccatg cgcgtggtac ggcagaacct cacctgggcc ttcgtctata
      481 acgctgtttg cgtgccgctg gctgtcgtcg gctacctgcc ggcctggctc gccggcctcg
      541 gcatggcgac cagctctctg ctggtggtgt tgaatgccgc gcgtctttcc gtgatcgcgc
      601 ccgttgccga aacgcaggcc gccgaagcac ccggaacgaa gctggaagag cgcgctgcgg
      661 gtgcgcggat ggctgcctga tggagatcct gttcctgttg atccccttgt cggtggtgct
      721 ggtactggcg atccttgccg ggctctggtg ggccatccac accgggcagt tcgaggatgt
      781 ggagaaggaa ggcgcgcgca ttctgcatga agattgattt gcatcaagtc gcccacgacc
      841 gacgcccggc agactgccgg taattcctgc cggaccggat gcccgggcgg gggaagccaa
      901 cctgtcactg ggagaacatc catggtttca aacaacaaga cagcagccca atacaacgat
      961 accgcgctcc gccaattctc catcatggcc gtcgtctggg gtgtcgtcgg catgctgatg
     1021 ggcgtcatca tcgccgccca gctcgtctgg ccggagctca atctcggcct gccctggacc
     1081 agcttcggtc gattgcggcc gctgcacacc aacgccgtga tcttcgcctt cggcggttcg
     1141 acgctgttcg ccacctctta ctacgtggtg cagcgtacct gccagaccac cttgttctgc
     1201 ccacgactgg cgagcttcac cttctggggg tggcaggcag tgatcgtcgc tgccgccatc
     1261 tcgctgccgc tgggcttcac ctccggcaag gaatacgccg agcttgaatg gccgatcgac
     1321 atcctgatcg ctgtggtctg ggtgtcgtac gcgatcgtgt tcttcggcac catcggccgg
     1381 cgcaaggtgc ggcatatcta tgtcgccaac tggttcttcg gtgccttcat catcgccgtc
     1441 gcgctgctgc atatcgtcaa cagcgccgag atgccggcga gcttcatgaa atcgtattcg
     1501 gcctacgccg gcgtgcagga tgccatggtg caatggtggt acggtcacaa cgcggtcggt
     1561 ttcttcctca ctgcgggctt cctcggcatc atgtattact tcgtgcccaa gcaggccgag
     1621 cgcccggtgt attcctacag cctctccatc gtgcacttct gggcgctgat cttcacctac
     1681 atgtgggccg gcccgcacca tttgcactac acggcgctgc cggactggac ccaatcgctg
     1741 ggcatggtgt tctcgctgat cctgctggcg ccaagctggg gcggcatgat caacggcatc
     1801 atgacgctct ccggcgcctg gcataagctg cgcaccgacc ccatcctgcg cttccttatc
     1861 gtgtcgctgt cgttctacgg catgagcacc tttgaaggcc cgatgatggc cgtgaagacc
     1921 gtcaacgcgc tgtcgcatta caccgactgg accgtaggcc acgtgcactc cggtgcgctg
     1981 ggctgggtcg ggctggtgtc gatgggcgcg ctctactaca tggtgccgcg cctgttcggc
     2041 cagaagcaga tgcacagcat caaggccatc gaagtgcatt tctggatggc gaccatcggc
     2101 atcgtgctct acatctccgc gctctggatc tccggtgtga tggaaggcct catgtggcgc
     2161 gccatcaacc ccgacggcac gctgacctat accttcgtcg agagcgtcaa ggccaagcac
     2221 ccctattacc tgttgcgcct gctcggcggg ctgctctatc tgggcggcat gctggtcatg
     2281 ctgtggaacg tgctcaagac cgccagcaag ggccaggcgg tggtgaccga tatcccgcca
     2341 gtcgcggcac acgcttaagg agccatcatg acgcaagaaa aaaccgggtt cacccacgag
     2401 aagatcgaga ccagcaactt cctcatgatc gtattgatcc tgctggtggt cgccatcggc
     2461 gggctggtcg agatcgtgcc actgttcttc cagaaatcga ccaccgagcc gatcaagggc
     2521 gtggagccct acaccgcgct gcagttggcg ggacgcgata tctatgtgcg agagggctgc
     2581 tataactgcc actcgcagat gatccggcca ttccgcgccg agacgctgcg ttatggccat
     2641 tattcggtgg ctggcgagtt cgtctacgac catccgttcc agtggggcag caagcgcacc
     2701 gggccggatc tagcgcgtgt cggcggccgt tacagcgacg actggcaccg tatccacctg
     2761 accaatccgc gtgacgtcgt gcctgaatcc atcatgcccg agtacccatg gctggagaag
     2821 acgctggtcg atgctgaggc catgccgtcg cacatgcgcg cgctgcgcgt ggttggcgtg
     2881 ccttataccg atgcacagat cgcgggttcg gtggatgagg tcaagggcaa gaccgagatg
     2941 gacgccatga tcgcctacct gcagatgctc ggcacccacc ttaaatagga gacctccatg
     3001 gatatcaata ccttacgcat ccttgtcacg gtcgtcagct tcgtcttgtt cgtcggcatc
     3061 gtgatctggg catggcgcgg ccgcaacacc caggattttc gccaggcagc gaatctgccg
     3121 tttgaagagg aatagggagt caacatgagc gatttcgtca gcaatttctg gccggtgttc
     3181 atcacgctgg tgacgctagc cggcatctac ggctgcggcc tcctgctgtg gaagaccggc
     3241 aaggtgaagg tcatcaaggg cggcgacggc accagcggcc acgtctggga tggcgacttg
     3301 cgcgagatga acaatcccct gcccaagtgg tgggtcggcc tgttcgtcat caccatcgtc
     3361 ttttccctgg tctacctggc ggcgtatccg ggtctgggca agtatgaggg gcagctgggc
     3421 tggacacagg tgcgccagta cgaggaagaa gtcaaagccg cagaccagtc cctggcgccg
     3481 ctctatgccc gatttgcggc catggacgtg gcagcgctga gtcaggacac cgaagcccac
     3541 gccatcggcg agcggctgtt catgaacaac tgcgcgcaat gccacggttc ggatgcgcgc
     3601 ggcagtcgcg gctttcccaa cctcaccgac catgactggc tgcatggcgg cacgccggag
     3661 atcatcaagc agaccatcag cgcgggccgc atcggcatga tgccgccgat ggccgccgca
     3721 gtgggtaacg acgaggacgt gaagaacgtc gccaattacg tgctgagtct ttccggcagc
     3781 gcccatgacg cccagcgcgc cgcactcggc aaggacaagt tcgtggtctg tgccgcctgc
     3841 catggtgccg aaggcaaggg caacacgatg atcggcgcac ccaatctcag cgaccatatc
     3901 tggctgcacg gcagcggcga ggaagccatc atcagacgca tccgcgaagg caagaccaac
     3961 cagatgccag cctggagcgg caaattcacg gaagcgcaga tccatgtgct gactgcgtat
     4021 gtctggggac tctccaacca gggcgcgcct gtcggcggcg atgacaggga tgctgctggc
     4081 gaaaaggata gcggcgaaaa agacagcggc gaaaaagaca gcggcgaaaa aaatagcacc
     4141 gaagacaagc ccgccgtcgc agacggacac gacagtgact gatgtgaacg gcgttatcga
     4201 taaagccgtg accggcgata cgggaaaagc cgtgaccggt acggccgacg aggcgctggt
     4261 atcgctgtat gcctcgcagg aaaaagtcta cccgcgcagc gtctccg
//
LOCUS       DATMTW010000103         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_579240, whole genome shotgun sequence.
ACCESSION   DATMTW010000103 DATMTW010000000
VERSION     DATMTW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579240"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VN084_01995"
     CDS             complement(<1..478)
                     /locus_tag="VN084_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWT28254.1"
                     /translation="MVKKTTKKQPATAVITHRVNPALMAQYEQWLDEIVPVAERYPGH
                     VGTVIIRPALTGEVDYRIVLHFDTRAHLRLWLASQDRAALIAKAELLLANAEQVELRQ
                     EAAIWFNLVSATTPTPRRWKQVLVTWSALYPLVLTVSLLVGWLTHYLGALQHDSLRI"
     gene            complement(551..1297)
                     /locus_tag="VN084_02000"
     CDS             complement(551..1297)
                     /locus_tag="VN084_02000"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWT28255.1"
                     /translation="MSQKLANKVAVVTGASKGIGAAIAKHLAGEGAAVVVNYSASQAA
                     AEQVVAEITRAGGKAIAVKANVSEQADIARLFSATQNAFGPVDILVNNAGVYNPAPIE
                     EINADNFYKHIDLNMLGLLLTTQEALKHFNPQGGSIVNISSVVSTLGFPGLSVYSGTK
                     GAVDAYTRSLAKEFGPRNIRVNSVNPGMVETEGLHDTGFIAQKDQMAAVTPLGRIGQP
                     EDIAPAVTFFASADASWITGETLLISGGLR"
     gene            1446..2360
                     /locus_tag="VN084_02005"
     CDS             1446..2360
                     /locus_tag="VN084_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWT28256.1"
                     /translation="MDRFDAMQAFLRVVEMHSFTKAAQSLNLPKATVSAQVQALEKRL
                     NVRLLHRTTRNISITKEGVAYYERAKQLLVDLEETEAAVSLSSRSYRGRLRVETSGTL
                     GRQILIPALDDFFRRYPEIDLEIGCSDRTVDLLQDGVDCAIRGGMPLDDTLVARKLIE
                     TQPVTCATQTYLDRHGTPQTLEELQQHNVVNYLSPRTGRVVGFNYLTGDDKVTIYGRR
                     RVTINDIDACLSAALADLGIVQMPYFAVRHHIKAGRLLRVLAQYPAECTPVHIVYLPN
                     RHLSANVRAFVDWVVELFHREATDYASM"
BASE COUNT          459 a          725 c          712 g          488 t
ORIGIN      
        1 ggatgcgcaa gctgtcatgc tgcagcgctc ccaggtaatg ggtgagccag cccaccagta
       61 gcgacaccgt caacaccagt ggatacagcg ccgaccatgt caccagcacc tgtttccagc
      121 ggcgtggcgt tggggtggtg gctgagacaa ggttgaacca gatggccgct tcctgacgca
      181 gctccacctg ctcggcgttt gccagcagga gttcggcctt ggcgataagt gccgcacgat
      241 cctgcgaggc gagccacaag cgtagatgcg cccgggtatc gaagtgcagc acgatgcggt
      301 aatcgacttc cccggtcagc gccgggcgaa tgatcaccgt acccacgtgg ccggggtagc
      361 gctccgctac cggtacgatc tcgtccagcc actgctcata ctgcgccatc agcgcagggt
      421 tgacacgatg cgtgatgacc gcggtagctg gttgtttttt cgtcgttttc tttaccatga
      481 tcgatcccct tctggatcat tccctttcga taacaggcgg gatgacacga tagccatccc
      541 gctatgctgg ttaacgcaat ccgccgctga tcaacagggt ctcgcccgtg atccacgacg
      601 catctgccga ggcgaagaag gtcacagccg gggcgatgtc ttccggctgg ccgatgcggc
      661 cgagcggggt gacggcggcc atctggtcct tctgcgcgat gaagccggta tcgtgcaggc
      721 cttcggtttc caccatgcct gggttcactg aattcacgcg gatattgcgc gggccgaatt
      781 ccttcgccag tgagcgcgtg taggcatcaa ccgcgccctt ggtgccgcta tagaccgaca
      841 ggccagggaa gcccagcgtg ctgaccacgg agctgatgtt gacgatgctg ccgccctgcg
      901 ggttgaaatg cttgagcgct tcttgcgtgg tcagcagcag gccgagcatg ttgagatcga
      961 tatgcttgta gaaattatcg gcgttgatct cctcgatcgg cgccgggtta tagacacctg
     1021 cgttgttcac caggatatcc accgggccga aggcgttctg ggtggcgctg aacaggcgcg
     1081 cgatatcggc ctgctcgctg acgttggctt tcacagcgat ggccttgccg ccagcacggg
     1141 tgatctcggc caccacttgc tcagccgccg cctggctcgc ggaatagttg acgacgacag
     1201 cagcgccctc gccagcgaga tgcttggcga tcgctgcacc aatgcctttg gatgcgccgg
     1261 tgaccacggc aaccttgttt gcaagtttct gactcatgat gttgctcctt tgattatcgg
     1321 gtttcggttg gcgctcgtga tcgatcgctt caccatggga accagtgtat tgatatgccg
     1381 tgacaagata aataatgata aattagaaat actgtattcg tttattggac aatcacggga
     1441 acatcatgga ccgattcgat gccatgcaag cctttctgcg agtggtggag atgcatagct
     1501 tcaccaaggc cgcgcaatcg ctcaacctgc ccaaggccac cgtctcggcc caggtgcagg
     1561 cacttgagaa gcggctcaac gtgcggctgc tgcaccgcac cacgcgcaac atcagcatca
     1621 ccaaggaagg cgttgcctac tacgagcgcg caaaacaatt gctggtggat ctggaagaga
     1681 ccgaagccgc tgtcagcctc agcagccgta gctaccgcgg ccgcctgcgg gtggagactt
     1741 cgggcacgct gggccggcag atcctcatcc ccgcgctgga tgatttcttt cgccgctatc
     1801 cggagatcga tctcgagatc ggctgcagcg accgcacggt ggatctgttg caggatgggg
     1861 tggattgcgc cattcgcggc ggcatgccgc tggacgatac gctggtggcc cgcaagctga
     1921 tcgagaccca gcctgtcacc tgcgccacgc aaacctatct cgaccgccac ggcacgccgc
     1981 agacgctgga agaactgcaa caacacaacg tggtgaacta tttatcgccg cgcaccgggc
     2041 gcgtggtcgg cttcaactat ctgacgggtg acgacaaggt cacgatctat ggtcgccgcc
     2101 gcgtcaccat caatgacatc gacgcctgcc tctccgcagc gctggctgat ctgggcatcg
     2161 tgcagatgcc gtatttcgcc gtgcgccacc atatcaaggc cggccggctg ttgcgcgtcc
     2221 tggcgcaata tcccgccgaa tgcacaccgg tgcacatcgt ctacctgccc aaccggcatc
     2281 tctcggccaa tgtgcgcgcg tttgtcgatt gggtggttga attgttccac cgggaagcga
     2341 ccgactacgc cagcatgtga cgcaaaatcg cccggcatga cgcg
//
LOCUS       DATMTW010000104         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_478895, whole genome shotgun sequence.
ACCESSION   DATMTW010000104 DATMTW010000000
VERSION     DATMTW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478895"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..628
                     /locus_tag="VN084_02010"
     CDS             188..628
                     /locus_tag="VN084_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWT28257.1"
                     /translation="MTLRLDYYSASPKAMKALIAMEALTSNLSIEPALLHLIKIRASQ
                     INGCAFCTDMHSVDARRLGETDRRLYSIVVWRDSNFFNPRERAALAWTEAVTLLCESH
                     VPDAVYEQARTQFSEGELVDLTIAVTTINSWNRLAVSFRQTPSA"
     gene            702..1679
                     /locus_tag="VN084_02015"
     CDS             702..1679
                     /locus_tag="VN084_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWT28258.1"
                     /translation="MSFMENRGDQPVAQYPTHLDTTASWRTRAAQIDPLLAQCFSVIA
                     RCGCFMQASRRLNIKATRLRKHLAQLEKQLRCSLFSPSENGLVLSRDGLQLHKQLTAL
                     AHERDLPVLEQPVVRLAVAETILHDILGRDLVALLRRNASARLDIITLDSELSLQAVS
                     ADVVVWMTGIGSPTPGPSFAVNEPRCLARLDYLPHIAKRYSRVAARPAGLDDLADFML
                     VQWQHDRQIDSFRSWNDLVDQRLAGVVQSHSYPLMLEMVRCSACIGLLPAYMSYFDRG
                     LIGLPGLFSQPMQRQVWLAVNAEAEGDAQVQTIVELILGTFEERREWFL"
     gene            1856..2377
                     /locus_tag="VN084_02020"
     CDS             1856..2377
                     /locus_tag="VN084_02020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWT28259.1"
                     /translation="MTGTDAVITLERLNHSHVPGITALYHEPVVARQTLQMPFPSSET
                     WQKRVETDNERIVQLVALHQGTVIGSITLEQFPRIRRSHAGSFGMGVASRWQGKGVGS
                     KLLAAVLDIADNWMNLRRVELSVYADNEAAISLYRKFGFETEGLFRDYAVRDGVYVDT
                     LSMARLRRMPKCD"
     gene            2470..2964
                     /locus_tag="VN084_02025"
     CDS             2470..2964
                     /locus_tag="VN084_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28260.1"
                     /translation="MDDVRQLGEMLRHYADSEAHKKQLFETQSAEWAKRIGELFDQIQ
                     QWLEPVLAPNLLEVSREAYVASGPSVPVETSTFKTEKLSITIAGKPVEFVPDVMGAGG
                     QISLAVMGFTAARYGSVSLVCMPSSNGWQWRKTNGLKDPDTFVFDADFLAQQLQSLIP
                     RERG"
BASE COUNT          620 a          925 c          953 g          631 t
ORIGIN      
        1 cgattgaggc gcgtgtgcgt tacgccagat taccctcaac tggcgtttat gcagtagttg
       61 cgtggcgtaa aaaccgccaa ttggtctggg cccaggtggt ccacggaaat ttcgcgcaat
      121 tggatatgta acgcagcgtt aacaacgtct accttggcgg catatcgacg ccatatgagg
      181 tcacgccatg accctccgcc tggattatta cagcgcctcg cccaaggcga tgaaagcgct
      241 gatcgccatg gaagccctga ccagcaacct gagtatcgag ccggcgctgc tgcacctgat
      301 caagatccgt gcttcgcaga tcaatggctg tgcgttctgc actgacatgc actcggtaga
      361 cgcccggcgc ctgggcgaaa ccgaccgccg cctgtactcc atcgtggtct ggcgcgacag
      421 taacttcttc aacccacgcg aacgcgccgc cctggcttgg acggaggcgg tgacactact
      481 ttgcgagagc catgtgccgg acgccgtcta cgagcaggcg cggactcagt tcagcgaagg
      541 tgaactggtg gacctgacca tcgctgtcac caccatcaac agctggaatc gactggccgt
      601 gagttttcgc cagaccccca gcgcctgaaa ggctgtcaca cgtcgttcat gtaccttgcg
      661 caggatggtg ctcccagtga aagatcgaca gggagtcatc aatgtcattc atggaaaacc
      721 ggggcgacca gcctgtagcg caatatccca cacacctcga tacgaccgcc tcctggcgca
      781 cccgcgcggc gcagatcgat ccgctgctcg cgcagtgctt cagcgtgatc gcccggtgcg
      841 gctgcttcat gcaggcatcg cggcgcctca acatcaaggc aacccggttg cgcaagcatt
      901 tggcacagct tgaaaaacag ctgcggtgtt cgctgttcag cccatcggag aatggcctgg
      961 tactcagtcg tgacggcctg caactgcaca agcagctgac cgccctggcg catgaacgcg
     1021 acctgccggt gctcgagcaa cctgtggtgc gcctggcggt ggccgagacg atcctgcacg
     1081 acatcctcgg tcgcgatctg gtggcgttgc tgcgacgcaa tgccagcgcg cgcctggaca
     1141 tcattaccct cgacagcgaa ttgtccctgc aggcggtcag tgccgatgtc gtggtatgga
     1201 tgacaggcat cggctcgcca acgccgggac cgagttttgc cgtcaacgag cccagatgcc
     1261 tggcgcggct cgactatcta ccgcacatcg ccaaacgcta ttcacgagtc gccgcgcggc
     1321 cggccggtct cgatgacctg gccgatttca tgctggtgca atggcagcat gatcgacaga
     1381 tcgacagttt tcggtcctgg aacgacctgg tcgaccagcg cctggccggg gtcgtgcagt
     1441 cgcattccta tccactgatg ctggagatgg tccgttgcag cgcctgcatc ggcctgttgc
     1501 ctgcctacat gagctatttc gaccgtggct tgatcggttt gccggggttg ttcagccagc
     1561 cgatgcagcg ccaggtgtgg ctggcggtga atgccgaggc cgagggcgat gcgcaggtgc
     1621 agacgattgt cgagttgatc ctcggcacct tcgaagagcg gcgggaatgg ttcctgtagg
     1681 agcgagcatg ctccgggcgg cgttccgacg aaaaacctgg gagcgccgcg gggcatcagg
     1741 tatcccgcgt catcgttaac gaccatcgcg agcattctcg ctcctacagg gtgtttttcc
     1801 atgttggatg cgttgtagag tgaacggcca tgtctctatc aaggatgaat cacccatgac
     1861 cggcaccgac gctgtcatca ccctcgaacg cctcaaccat tcccacgtcc cgggcatcac
     1921 cgcgctttac cacgaaccgg tcgtcgcccg tcagacgctg caaatgccgt ttccatcctc
     1981 cgagacttgg caaaaacgcg tggagacgga caatgaacgg atcgtacaat tggtcgccct
     2041 gcatcaaggg acggttatcg gtagcatcac cctggaacag tttccacgca tccgccgcag
     2101 ccacgcaggc agcttcggca tgggcgttgc ctcccgatgg cagggcaagg gcgtgggttc
     2161 gaaactgttg gcggcggtgc tggatattgc tgacaactgg atgaacctgc gacgggtcga
     2221 actgagcgtg tatgccgaca acgaagccgc catcagcctg taccgcaagt tcggtttcga
     2281 gaccgaaggg ctgtttcgcg actatgcggt gcgcgatggc gtctacgtcg ataccttgag
     2341 catggcgcgc ttgcgccgca tgcccaagtg cgattgatcc ataccactcg cgcttttagt
     2401 cgtacgccgg cggcgcgcat ctgccatgct caatgtccgc agcaccgctt caatcaaagg
     2461 aacaccgcaa tggacgacgt acggcaactg ggcgagatgc ttcgtcacta cgcagacagc
     2521 gaagcgcaca aaaagcagct gttcgagaca caatcggccg agtgggccaa gcgcattggg
     2581 gagttgttcg atcagatcca gcagtggctg gagccggttc tggcaccgaa tctgctggag
     2641 gtgagccgcg aagcgtatgt cgcgtccggg ccgagtgttc cggtggagac gtctaccttc
     2701 aagactgaaa agctgagcat tacgatcgcc ggcaaaccgg tggagttcgt gccggatgtg
     2761 atgggtgccg gtgggcagat ttccctggca gtgatgggct ttaccgctgc gcgctacggc
     2821 agtgtttcac tggtgtgcat gccatcgtcc aatggctggc agtggcgcaa gaccaatggt
     2881 cttaaagatc cggatacctt tgtcttcgat gcggatttcc tggcgcagca gttgcagagc
     2941 ctgattccgc gcgaacgcgg ctgagttttc gcgacactgt gaatccccct gtgggagcga
     3001 gcctgctcgc gaaagcgatg aatcagtcaa tatcgatgtc gactgtcagc ccgtcttcgc
     3061 gagcgggctc gcttgtatgg taggacttga agggaggagg agggacaagg ctcgattctg
     3121 actgttgac
//
LOCUS       DATMTW010000105         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_42701, whole genome shotgun sequence.
ACCESSION   DATMTW010000105 DATMTW010000000
VERSION     DATMTW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42701"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..>1596)
                     /locus_tag="VN084_02030"
     CDS             complement(292..>1596)
                     /locus_tag="VN084_02030"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28261.1"
                     /translation="ASFAFLQTLSFPGGYAPAGLIGDGTNTSAWFFVFWHVTYPLGIL
                     IHALSKDAPVRTMRLDRSAEAAIGVTVVSVFVVVTGLAWLATAHIESLPVFYRDSVTL
                     QTRLGNQVNLGFLLWYAVVLVVLLVRRRTILDIWLAVILIAWMPNFVVAMLASSVRFS
                     FGWYASRGFALVASFMLLSVLLTEMTVLYSRLANAFSLLRRERANRMMSVDAATAAIA
                     HEVRTPLGAIALNADVALVHLRSTPPELAEVGFILKDIEADALRANEVISGIRALFKT
                     EPGNRLPVNLENLVRHAVALSEPDLLASGIAVSTDFRADQATVQGDPTQLQQVVLNLI
                     RNAVDAMGTTARDDRQLSFSTETKDGSTVVLSVCDTGAGIREEDQDRIFDAFFTKKTT
                     GMGLGLAISRAIVDGHEGTLRLAKSDPNGSEFEIELHRLRVR"
BASE COUNT          348 a          476 c          462 g          310 t
ORIGIN      
        1 atccagctcc gcttgtttca agcagccagt tatggcgttg gggtcttcgg cctgaaggca
       61 aacctgtcgc tttcgacatg ctcgccgtcg atccgcgatg caactagatg cgccaggcct
      121 gacttcccta cgccgtcgaa accctcgatg cgcaggagtc tcggatcgct cgcctcgcgc
      181 caagcacgga tcactacgtc tgcggcttcc tcgaaggtcc gaaccgtcga tatcttcacg
      241 ttaatcgttc ctagtccgcc ggcgatcacc ggctctaaga taactggccg actaccggac
      301 acgcagcctg tggagctcga tttcaaattc tgatccgttc ggatcagatt tcgccagacg
      361 taacgttcct tcgtgaccat ctacaatggc gcggctgatg gcgagaccca accccatgcc
      421 tgtcgtcttc ttagtgaaga atgcatcaaa gatacgatcc tgatcctcct cgcggatgcc
      481 ggctccggtg tcgcatacgg acagcacgac cgtcgatccg tccttcgttt cagtcgaaaa
      541 gcttaactgc cggtcatcgc gggccgtagt gcccatggca tcgactgcgt tcctgatcag
      601 gtttagaacc acctgctgca actgggtagg atcgccctgc acggttgctt ggtccgcgcg
      661 gaaatcggtc gatacggcaa tgccgcttgc gagcaaatca ggctcggaca gggcgactgc
      721 gtgccggaca aggttttcaa ggtttacggg gaggcggttg ccgggctcgg tcttgaacag
      781 tgcgcggatg cccgaaatga cctcgtttgc ccgcagtgcg tccgcctcga tatccttgag
      841 aatgaacccg acctcggcaa gttcgggggg cgtggaacga aggtggacga gcgcaacatc
      901 tgcattgagc gcgatagcgc cgagcggggt gcgcacctca tgcgcaatcg ctgcggtggc
      961 cgcgtctacg ctcatcatcc ggttggcccg ctcgcggcgc aataacgaaa aggcgttggc
     1021 aaggcgcgaa tacagcactg tcatctcggt cagtagcacg ctcagcagca tgaagctcgc
     1081 aacaagcgca aagccgcgag aagcatacca gccgaacgaa aatcgtaccg agctggcaag
     1141 catagcgacg acgaaattgg gcatccatgc tattaggatc acggctagcc agatgtcgag
     1201 gattgtgcgc ctgcggacca gcaacaccac aaggacgact gcgtaccaga gcaggaagcc
     1261 gaggttcacc tggttgccca gtctggtttg cagggtgacg ctgtccctgt agaagaccgg
     1321 caggctttca atatgcgctg tagcaagcca tgccagtcca gtgaccacca cgaacacgct
     1381 gacgaccgtc acaccaatgg cggcttctgc agaccgatcc aatcgcatgg tgcgaaccgg
     1441 cgcatccttc gagagtgcgt ggatcaggat accgagcggg tacgtcacgt gccagaaaac
     1501 gaaaaaccaa gctgatgtat tggttccgtc tccgatcaga cccgccggcg cgtagccacc
     1561 gggaaagctc agggtctgca ggaaggcaaa tgatgc
//
LOCUS       DATMTW010000106         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_42755, whole genome shotgun sequence.
ACCESSION   DATMTW010000106 DATMTW010000000
VERSION     DATMTW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42755"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /gene="mpl"
                     /locus_tag="VN084_02035"
     CDS             <1..1338
                     /gene="mpl"
                     /locus_tag="VN084_02035"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HWT28262.1"
                     /translation="DQTSLNPDIYVIGNVVTRGNPLMEAILDRGLPYISGPQWLAENV
                     LQGKWVLAVAGTHGKTTTASMLAWILEYAGLAPGFLIGGVPQNFGISARLPGISPKDS
                     NYHLYKQQDLAVDTRRPAHSGRDSTDGYGEAVSTAQQSDAGKDAVCSPFFVIEADEYD
                     TAFFDKRSKFVHYHPRTAILNNLEFDHADIFADLAAIETQFHHLVRTVPGQGLVVANG
                     REESLQRVLQRGCWTPVETFGDSGGWQAGEVHQDGSFDVLHAGKFQGRVQWELLGEHN
                     RMNALATLAAARHVGVPVEVGIAALSTFQNVKRRMEVRGVVNAVTVYDDFAHHPTAIA
                     TTVAGLRAKVGHARILAVLEPRSNTMKLGVMKAALPDSLVDADQVFCYGANLGWDAAE
                     AFAPIAHKAKVYDDMPAMVEAIVASARPGDHVLVMSNGGFGGVHQKLLERLGT"
     gene            1350..>2235
                     /locus_tag="VN084_02040"
     CDS             1350..>2235
                     /locus_tag="VN084_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWT28263.1"
                     /translation="MKNWEPLEITLAVSAFLHLVVIVAVKFEPPDLKFFKDKMPPLEV
                     VLVNAKTEKAPTKADALAQANLDRGGNTEENRRLKSALPPPKQQVTEVSVKPAAEAKK
                     AAAAAEAQAEAERKQQQRVAELERQAQELMTQINAKKKVETAPPKPSAAPEIDKQQQQ
                     ATSKLSGADLMASSLDIARLEAQIAKQQDEYQKRPKRKFIGARTQEYRFATYVEAWRQ
                     KVEKVGNLNYPEAAKEQKIYGQLRMTVSIKADGNIESIEVNSSSGFKVLDDAAKRIVE
                     LAAPYAAFPEDIRKDTDIL"
BASE COUNT          485 a          663 c          695 g          392 t
ORIGIN      
        1 gaccagacca gcctcaatcc cgatatctac gtcatcggta acgtcgtcac gcgcggcaat
       61 ccgttgatgg aagccatcct cgatcgtggc ctgccctata tctctggccc gcaatggctg
      121 gccgagaacg tgctgcaagg caagtgggtg ctcgctgtcg caggtaccca cggcaagacc
      181 accaccgctt ccatgctggc ctggatcctc gaatacgcag gtcttgcgcc tgggttcctc
      241 atcggcggcg taccccagaa tttcggcatc tcggcgcgct tgccggggat ttcaccaaaa
      301 gacagtaatt accatctcta caagcagcaa gatttggctg tcgacacgag gcgcccggcg
      361 cacagcggcc gagacagtac agatgggtac ggcgaggcgg tgagcaccgc gcaacaaagt
      421 gacgcaggca aagatgctgt ttgttcgccg ttcttcgtca tcgaggcgga cgaatacgac
      481 accgccttct tcgacaagcg ttccaagttc gtgcactacc atccgcgcac ggccatcctc
      541 aacaacctcg aattcgacca cgccgatatc tttgccgatc tcgccgcgat cgagacccag
      601 ttccatcatc tggtgcgtac ggtgcccggt caggggctgg tggtggccaa tggccgcgaa
      661 gagagtctgc agcgcgtgct gcaacgtggc tgctggacgc cggtagagac cttcggcgat
      721 agcgggggct ggcaggcggg tgaggtccat caggacggta gcttcgacgt tttgcatgcc
      781 ggcaaatttc agggccgcgt gcaatgggag ctgctgggcg agcacaatcg catgaacgcg
      841 ttggcgacgc tggcggcggc acgtcatgtc ggcgtaccgg tcgaggtcgg catcgcagcg
      901 ctgagtacct tccagaacgt caaacgccgg atggaagtgc gtggcgtagt caatgctgtc
      961 acggtctatg acgatttcgc ccatcatccg acggcgatcg ccacgacggt cgccggcctg
     1021 cgtgccaaag tcggccatgc gcgcatcctg gccgtgctcg agccgcgctc gaataccatg
     1081 aagcttgggg tgatgaaggc tgccttgccc gatagcctgg tcgatgccga ccaggtgttc
     1141 tgctatggcg ccaacctggg ctgggatgcc gcagaagcct tcgcgccgat cgcgcacaag
     1201 gcgaaggtct atgacgatat gcccgccatg gtcgaggcca tcgttgcgag cgcccggcca
     1261 ggcgaccatg tgctggtgat gagcaacggc ggttttggtg gcgtgcacca gaagttgctg
     1321 gaaaggctgg gcacttgagg cctgccttca tgaaaaactg ggaaccgctg gagatcaccc
     1381 tggcggtctc ggcttttctg catttggtgg tgatcgtcgc cgtcaagttc gagccgcctg
     1441 acctcaagtt cttcaaggac aagatgccgc cgctggaagt ggtgctggtc aatgccaaga
     1501 ccgaaaaggc cccgaccaag gccgatgcac tggcgcaggc gaaccttgat cgcggcggca
     1561 atacggaaga gaaccgtcgt ctcaaatcgg ctttgccgcc tcccaagcag caagtgactg
     1621 aagtctcggt caagccggcg gccgaggcga agaaggcggc cgcagctgcc gaagcgcagg
     1681 ccgaagccga acgtaagcag caacagcggg tcgccgaact cgagcggcag gcgcaagagc
     1741 tgatgaccca gatcaacgcc aagaagaagg tggagaccgc gccacccaag ccatcggcgg
     1801 cgccagagat cgataagcaa caacagcagg cgacctcaaa actcagcggt gccgatctca
     1861 tggccagcag tctcgatatc gccaggctcg aggcgcagat cgccaagcag caggacgaat
     1921 atcagaaacg tccgaagcgc aaattcatcg gcgcgcgcac ccaggaatac cgtttcgcga
     1981 cctatgttga agcctggcgc cagaaggtgg agaaagtggg caaccttaat tatccggaag
     2041 cagccaagga gcagaagatc tatgggcagc tccgcatgac ggtttccatc aaagccgatg
     2101 gcaacatcga gagcatcgag gtcaactcga gttccggttt caaggtgctg gatgacgcgg
     2161 cgaaacgcat cgtcgaactg gccgcgccct atgccgcgtt tcccgaggat atccgcaagg
     2221 atacggatat cctga
//
LOCUS       DATMTW010000107         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_613266, whole genome shotgun sequence.
ACCESSION   DATMTW010000107 DATMTW010000000
VERSION     DATMTW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613266"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VN084_02045"
     CDS             <1..854
                     /locus_tag="VN084_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWT28264.1"
                     /translation="AGPMAKSCEDMALMMNVMTGFDERDSTSLQREKEDYTRGLYAMD
                     PARPLAGLRVGLPREYFAEGLDAKVAKVVQDAIEQYRKLGAEIVDISLPNNNLSIPVY
                     YVLAPAEASSNLSRYDGVRYGHRAAEYTDLMDMYCKSRAEGFGAEVKRRILIGTYVLS
                     AGYYDAYYLKAQQIRRLIAEDFARAFEQCDVIMGPAAPSTAFRTGEKTSDPVAMYLED
                     LYTIAVNLAGLPGMSIPAGFAEVDGKQLPVGLQVIGNYFDEARMLDIGHAYQQVTDWH
                     TRMPEGV"
     gene            856..2289
                     /gene="gatB"
                     /locus_tag="VN084_02050"
     CDS             856..2289
                     /gene="gatB"
                     /locus_tag="VN084_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWT28265.1"
                     /translation="MEWEVVIGLETHTQLQTRSKIFSGASTTYGAEPNTQACEVSLAL
                     PGVLPVLNKEAVKCAIKFGLAIDAEIAPRSVFARKNYFYPDLPKGYQISQYELPVVGK
                     GKLTIQIGDTEKTINITRAHLEEDAGKSVHGASEGMSGIDLNRAGTPLLEIVTEPDMR
                     SAAEAVAYAKKLHELVQWIGICDGNMQEGSFRCDVNVSVRPKGSDKLGTRREIKNLNS
                     FRFMQQAIEYETRWQIETLEDGGTIQQATVLFNPDTGETRAMRSKEEANDYRYFPDPD
                     LLPLEITEADKAEVRVMMPELPQAMKARFEQEYALSAYDASALTASKSVADYFIATVQ
                     AFGGEAKLAANWVMGGISAKLNEEDKDITGSPVSPAQLAGLLKRIADNTISSKIAKEV
                     FEAMWAGEGEADAIIEKKGLKQVTDSGAIEAMIDEVLAANAAMVEEFKAGKEKAFNAL
                     VGQAMKASRGKANPAQVNEILRKKLGA"
     gene            complement(2305..2958)
                     /locus_tag="VN084_02055"
     CDS             complement(2305..2958)
                     /locus_tag="VN084_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWT28266.1"
                     /translation="MTAARLCMIGCTHILLSLSIVAGSACAESGKIVKWVDEKGVTHY
                     GDVVPPQYSGRDSTVINRQGMVIKRNLVNDQQVDPAEAQQAAEQQRRDRALRDAYTTE
                     EEIDLAKERNLQMDEASMLGLQQSLSSAKNRQLMLKKSVDDLAKRKKPQPEDLKKDVK
                     DNEAEIAKIERQMAERRKAMDATRQRFDNDKQRFRELTQKEAAPQPIKVAPSPAAAR"
     gene            complement(2970..>3257)
                     /gene="pyrE"
                     /locus_tag="VN084_02060"
     CDS             complement(2970..>3257)
                     /gene="pyrE"
                     /locus_tag="VN084_02060"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184195.1"
                     /note="involved in fifth step of pyrimidine biosynthesis;
                     converts orotidine 5'-phosphate and diphosphate to orotate
                     and 5-phospho-alpha-D-ribose 1-diphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWT28267.1"
                     /translation="VLIVDDVISAGTSVRESVEIITAAGSIPAGVAIAIDRQERGLGA
                     LSAVQEVERDHGLPVCAIANLDGLIRYLHDQSDMAQNLEQIQKYRTIYGIT"
BASE COUNT          653 a          977 c         1001 g          626 t
ORIGIN      
        1 aggcaggccc gatggccaag tcctgcgaag acatggcgct gatgatgaac gtcatgaccg
       61 gcttcgacga gcgcgattcg accagtctgc agcgcgaaaa ggaagactac acccgtggtc
      121 tctacgccat ggatccggcc aggccactcg ctgggctgcg cgtcggatta ccgcgggaat
      181 atttcgccga gggtctcgat gccaaggtcg ccaaggtggt gcaggacgcg atcgagcagt
      241 accgcaagct cggtgccgag atcgtcgata tcagcctgcc caacaacaac ctttccattc
      301 ctgtgtatta cgtgctggca cccgccgagg cgtcgagcaa cctgtcgcgc tacgacggcg
      361 tgcgctacgg ccaccgcgcc gccgaatata ccgacctcat ggacatgtac tgcaagagcc
      421 gcgccgaagg tttcggcgcc gaggtcaagc gtcgcatcct catcggcacc tatgtactga
      481 gcgccggcta ctatgacgct tactacttga aagcacagca gatccgccgc ctgatcgccg
      541 aggacttcgc ccgggcgttc gagcagtgcg acgtcatcat gggcccggcc gcgccgtcga
      601 ccgcgttccg cactggcgag aagaccagcg atccggtcgc catgtacctt gaggacctct
      661 acaccatcgc cgtcaacctc gccggtctgc ccggcatgag catccctgct ggtttcgccg
      721 aagtcgacgg caagcagttg ccggtaggcc tgcaggtcat cggtaactat ttcgacgagg
      781 cacgcatgct cgatatcggc catgcctatc aacaagtgac agattggcat acccgtatgc
      841 cggagggtgt ttaaggtgga atgggaagtc gtcatcgggc tggagaccca tacccagctg
      901 caaaccagat ccaagatctt ttccggggcc tcgaccactt acggcgctga gccgaacacc
      961 caggcctgcg aagtgagtct ggccctgccg ggtgtgctgc cggtgctgaa caaggaagcc
     1021 gtcaagtgcg ccatcaagtt cggcctcgcg atcgatgccg agatcgcacc gcgctcggtg
     1081 ttcgcgcgca agaactactt ctaccccgat ttgcccaagg gctaccagat cagccagtac
     1141 gaactgccgg tcgtcggcaa gggcaagctg accatccaga tcggcgatac cgaaaagacc
     1201 atcaacatca cgcgcgccca tctggaagaa gatgcgggca agtcggtaca cggcgccagc
     1261 gagggcatgt ccggtatcga cctcaatcgg gccggcaccc cgctgctaga gatcgtcacc
     1321 gagcctgaca tgcgttcggc ggccgaagcg gtggcctatg ccaagaagct gcacgaactg
     1381 gtgcagtgga tcggcatctg cgacggcaac atgcaggaag gcagtttccg ctgcgacgtc
     1441 aacgtctcgg tgcgtcccaa gggttccgac aagcttggta ctcgccgcga gatcaagaac
     1501 ctcaactcct tccgcttcat gcagcaggcc atcgaatacg agacacgctg gcagatcgag
     1561 accctggaag acgggggtac catccagcag gcgaccgtgc tgttcaaccc ggacacgggt
     1621 gagacgcgcg ccatgcgttc aaaggaagaa gccaacgact atcgttattt ccccgatccc
     1681 gacctgttgc cgctggagat cacggaagct gacaaggccg aagtccgggt gatgatgcca
     1741 gagctgccgc aggccatgaa agcccgtttc gagcaggaat atgcgctctc ggcctatgat
     1801 gcatcagcgc tgactgcctc gaaatcggtg gctgattact tcatcgcgac ggtacaggcg
     1861 tttggtggcg aagccaaact tgccgccaac tgggtgatgg gtggtatctc ggccaagctc
     1921 aacgaagaag acaaggacat caccggctcc ccagtgtctc cagcgcaact ggcaggcttg
     1981 ctcaagcgca tcgccgacaa caccatctcc agcaagatcg ccaaggaagt gttcgaagcg
     2041 atgtgggcgg gcgagggtga agcggatgcc atcatcgaaa agaagggcct gaagcaggtg
     2101 accgacagcg gcgccatcga agcgatgatc gacgaggtgt tggcggcgaa tgcggccatg
     2161 gtcgaggaat tcaaggcggg taaggaaaaa gccttcaacg cgctggtcgg tcaggccatg
     2221 aaggcttcca ggggcaaggc caacccggcg caggtcaacg agatcctgcg caagaagcta
     2281 ggcgcctagc gggcaacagc agggttagcg ggcagcggca ggtgaagggg cgaccttgat
     2341 cggttgcggg gcagcctctt tctgagtcag ctcccggaaa cgctgcttgt cgttgtcgaa
     2401 gcgctggcgg gtcgcgtcca tcgccttgcg gcgctcggcc atctggcgct cgatcttggc
     2461 gatctccgct tcgttgtcct tcacgtcttt ctttaggtct tcaggctgcg gtttcttgcg
     2521 cttggcgaga tcgtcgacac tcttcttcag catgagttga cggttcttgg cgctgctcaa
     2581 gctctgctgc aggccgagca tcgaggcctc gtccatctgc agattgcgtt ccttggcgag
     2641 atcgatctct tcttccgtgg tgtaggcatc cctgagcgcg cggtcacgcc gttgctgttc
     2701 tgcggcttgc tgggcctcgg ccgggtcgac ctgctgatcg ttgaccaggt tgcgcttgat
     2761 gaccatgccc tggcgattga tgactgtgct atcgcgtccg gagtattgcg gcgggacgac
     2821 gtcgccataa tgcgtgacgc ccttctcatc cacccatttg acgatcttgc cgctctcggc
     2881 acaggcgctc ccggccacta tcgatagcga taggaggatg tgggtacagc cgatcatgca
     2941 cagccgtgca gcagtcattc gttccccagt caggtgatac cgtagattgt tctatatttt
     3001 tgtatctgct cgagattttg cgccatatcg gattgatcgt gcaagtatcg gatcaggccg
     3061 tcgagattag cgatggcgca gactggcagg ccgtggtcgc gttccacctc ctgtaccgcg
     3121 gacagcgcgc cgaggccgcg ctcctgccgg tcgatggcga tggcgacgcc tgcggggata
     3181 gagccggcgg cggtgatgat ctccacggat tcgcgtaccg aggtgccagc cgatatcacg
     3241 tcgtcgacga tcagtac
//
LOCUS       DATMTW010000108         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_378123, whole genome shotgun sequence.
ACCESSION   DATMTW010000108 DATMTW010000000
VERSION     DATMTW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378123"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="nusA"
                     /locus_tag="VN084_02065"
     CDS             <1..964
                     /gene="nusA"
                     /locus_tag="VN084_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWT28268.1"
                     /translation="KENLRVGDRVRAYLSRIERGARGPQLILSRIAPEFLIKLFELEV
                     PEIEEGLLEIRSAARDPGLRSKIAVKSNDQRLDPVGTCVGMRGSRVQAVTSELAGERV
                     DIVLWSMEPAQFVINAMAPAEVTSIVVDEDAHSMDVVVDEEQLAQAIGRNGQNVRLAS
                     ELTGWTLNILTEEEAAKKHEEEYTNTRQLFMDKLDVDEEVADILVQEGFSTLEEIAYV
                     PLAEMSEIDAFDEDTINELRKRARAALLTEAIAKEEKVEEASEELLAMEGMDDATAHA
                     LAAKGVSTVEDLADLAVDDLVEMTEMDAERAKQLIMTARAPWFA"
     gene            1011..3659
                     /gene="infB"
                     /locus_tag="VN084_02070"
     CDS             1011..3659
                     /gene="infB"
                     /locus_tag="VN084_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWT28269.1"
                     /translation="MGQTSVAQFATELGLPAELLLEQLKGAGVNKAAADDKLTEQDKT
                     SLLEYLRKEHGAQAPKKQITLTRKQSTEIKKTDSTGKARTIQVEVRKKRVLVRRDDVA
                     ETEQIEEAPVELPVVEPELPEVVAEVAPPEPEVVIPEPEVIAPPVEEIEKPKAKLTRS
                     VLTPEQQAQREHEARRHAALIAAQTEDLRKKQELALKRQEEEAKRAAEKAAAAEKASK
                     LSEGTLHKLVAKEGAKEDDKNKKAGKSNRDWADNENKKRGIKTRGDVGVAQGWRAHKS
                     KSKSHKGDEDQQHAFNAPTEPIVHEVLVPETISVGDLAHKMAVKASEVIKALMKMGMM
                     VTINQVLDQETAIIVVEEMGHVAKAAAANDPETFLEEEAHAEAIMESRPPVVTVMGHV
                     DHGKTSLLDYIRRSRVASGEAGGITQHIGAYHVETPRGMVTFLDTPGHEAFTAMRARG
                     AKATDVVILVVAADDGVMPQTIEAVHHAKAAGVPLVVAVNKIDKPEANPERVKQELVA
                     QEVIPEEYGGETMFVEVSAKTGKGIDNLLEAVLLQAEVLELKAPKNTPAKGLVIEGRL
                     DKGRGPVSTILIQSGTLKRGDMLLAGTAFGRVRAMLDEAGREIKEAGPSIPVEILGLS
                     DVPNAGDEVIVLNDERKAREIALFRQGKFRDVKLARQQAAKLENMFEQMGEGEVKSLP
                     LIIKSDVQGSYEALATSLQKLSTDEVKVNIIHTGVGAISESDVNLGAASKAVVIGFNV
                     RADAGARKLIDSSGVDVRYYNIIYEAVDEVKAALGGMLSPEQKENAIGLVEIREVFRI
                     SKVGAVAGCYVLDGVIKRGSKVRVLRDNVVIHTGELDSLKRFKDDVKEVKASFECGLS
                     LKNFNDIEVGDTLEVFEVVEVARTLA"
     gene            3663..4031
                     /gene="rbfA"
                     /locus_tag="VN084_02075"
     CDS             3663..4031
                     /gene="rbfA"
                     /locus_tag="VN084_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWT28270.1"
                     /translation="MAKEFSRSDRVAEQIQRELADLLQFEVKDPRVGMVTITEVEVSG
                     DMSHAKIYYSAAEGTKELQKGLQKSAGFLRTQLSKRLLLRTVPQLHFVYDASIDRGMR
                     LSKLIDEALAPEAPTKSNKH"
     gene            4041..>4771
                     /gene="truB"
                     /locus_tag="VN084_02080"
     CDS             4041..>4771
                     /gene="truB"
                     /locus_tag="VN084_02080"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWT28271.1"
                     /translation="MQTKRIKRAIDGVLLLDKPLGFSSNQALQKVKWLYQAAKAGHTG
                     SLDPLATGLLPICLGEATKFSHFLLEADKSYRALIRLGSTTTTADAEGEVLTRSEVRV
                     DASQLADVLGSFVGDIVQVPPMYSALKHEGKALYEYARAGVDIPRKGREVTIHEIVLE
                     RFEGEMLEVVVRCSKGTYIRTLAEDIGHALGCGAHLAGLRRLTTAHLGLQDAVTIEQL
                     EALAPAARDALLLPVDATITDLPKVT"
BASE COUNT         1151 a         1266 c         1476 g          878 t
ORIGIN      
        1 caaagagaac ctgcgggttg gcgatcgggt gcgcgcctat ttgtcccgga tcgaacgtgg
       61 tgcccgtggc ccgcaactga tcctgtcgcg tatcgcgccg gaattcctca tcaagctgtt
      121 cgagctggaa gtcccagaga tcgaggaagg tttgttggag attcgctccg cagcccgtga
      181 tcctggcctg cgttcgaaga ttgcagtgaa atccaatgac cagcggctgg atccggtcgg
      241 cacttgtgtc ggcatgcgcg gttcgcgcgt gcaggctgtc accagtgaac ttgccggcga
      301 acgtgtcgat atcgtgttgt ggtcgatgga gccagcccag ttcgtcatca atgccatggc
      361 acctgcagaa gtgacgagta tcgtcgtcga tgaagatgcg cacagcatgg atgtggtcgt
      421 ggatgaagaa cagttggcac aggccattgg tcgtaacggc cagaatgttc gcctggcgtc
      481 tgaattgacc ggctggacac tgaacatcct gaccgaagaa gaagcggcca agaagcacga
      541 ggaagaatat accaatactc gtcagctgtt catggacaag ctggacgtcg atgaagaggt
      601 cgccgatatt ctcgtgcaag agggattcag caccctggaa gagatcgctt acgtgccatt
      661 ggccgaaatg agcgagatcg atgcgttcga tgaagatacc atcaacgagt tgcgcaagcg
      721 tgcacgtgca gccttgttga ccgaagcgat cgccaaggaa gaaaaggtcg aggaagcgtc
      781 cgaagaattg ttggcgatgg aaggcatgga cgacgctact gcgcacgctt tggctgccaa
      841 aggggtctca actgttgagg acctggcaga tctggcggta gacgatctgg tggaaatgac
      901 cgagatggac gctgagcggg ccaagcagtt gatcatgacc gcacgtgcgc catggttcgc
      961 ataaggctga aacagcgtaa gtagttagaa aagaaactgg agcaggaaat atgggacaaa
     1021 caagtgttgc gcaatttgcc accgagctgg gtctgcccgc tgaactgttg cttgagcaac
     1081 tgaaaggcgc gggcgtcaac aaggcggctg ccgatgacaa gctaaccgaa caggacaaga
     1141 cgtcgctgct cgagtacttg cgcaaggaac acggcgcaca agctcccaaa aagcagatca
     1201 cgctcacgcg caagcaaagt accgagatca agaagaccga cagcacgggc aaggcccgta
     1261 ccattcaggt ggaagtccgc aagaagcgtg tcctcgtgcg tcgagacgat gtcgccgaaa
     1321 ctgagcaaat tgaagaagct ccggttgaat tgccggtcgt tgagccagag ttgcctgaag
     1381 tggtcgctga agtcgcgcca ccagagcctg aggttgtgat tccagagccg gaagtaatcg
     1441 ctcccccagt agaagaaatc gagaaaccta aagccaagct cacccggagc gtactgacgc
     1501 cagagcagca ggcgcagcga gaacatgaag cgcgtcgtca tgcggccctg atcgcggcac
     1561 agacggaaga cctgcgcaag aagcaggaat tggcactgaa gcgccaggaa gaagaagcca
     1621 aacgtgccgc tgaaaaggct gcagcggccg aaaaagccag caagttgtcc gaaggcacac
     1681 tgcacaaact tgtcgccaag gaaggcgcca aggaagatga caagaacaag aaggcgggca
     1741 agagcaatcg cgactgggcc gataacgaga ataagaagcg tggtatcaag acgcgcggcg
     1801 atgttggcgt agcccagggt tggcgtgcgc acaagtcaaa atccaaatcg cacaagggcg
     1861 atgaagacca gcaacatgcc ttcaacgcac cgaccgaacc gatcgtgcat gaggtcctgg
     1921 tgccggagac catctctgta ggtgacctgg cccacaagat ggcggtcaag gccagcgaag
     1981 tgatcaaggc actgatgaag atgggcatga tggtcacgat caaccaggtg ttggaccaag
     2041 agactgccat catcgtcgtc gaagaaatgg ggcacgttgc caaggcggcg gcggccaacg
     2101 atcctgagac cttcctcgaa gaagaggcgc atgcagaagc catcatggag tcgcgcccac
     2161 cggtggtgac cgttatgggt cacgttgatc atggcaagac gtccttgctg gattacatcc
     2221 gtcgtagccg cgtcgcttct ggtgaagctg gcggcatcac ccagcatatc ggtgcttatc
     2281 acgtcgaaac cccgcgcggt atggtgacct tcctcgatac gcctggccat gaagccttca
     2341 cggcgatgcg tgcacgtggt gcaaaagcaa ccgatgtcgt cattctggtg gtggcggcag
     2401 atgatggcgt catgccgcaa accatcgagg ccgtgcatca tgccaaggca gccggcgtgc
     2461 ccttggtggt cgctgtgaac aagatcgata aaccggaagc caatcccgaa cgcgtcaagc
     2521 aggagctggt cgcccaggaa gtcatccctg aagaatacgg tggcgaaacc atgtttgtcg
     2581 aagtctcggc caagaccggc aagggtatcg acaacctgct ggaagccgtg ttgctgcaag
     2641 ctgaagtgct tgaattgaag gcgccaaaga atacgccggc caagggcctg gtgatcgaag
     2701 gccgactgga caaggggcgt ggcccggttt cgaccatcct catccagtcc ggcaccctca
     2761 agcgcggcga tatgctgctc gctggcaccg cctttggtcg cgtacgcgcg atgctcgacg
     2821 aagccggcag ggagatcaag gaagctggtc catccatccc ggtcgagatc ctcggtttgt
     2881 ccgacgtacc gaatgccggc gatgaagtca tcgtccttaa cgatgaacgc aaagcacgcg
     2941 agatcgcgtt gttccgccaa ggcaagttcc gcgatgtgaa gctggcaaga cagcaggcag
     3001 ccaagctgga aaacatgttc gaacagatgg gcgaaggcga agtgaagtct ctcccgctga
     3061 tcatcaagtc cgacgtgcaa ggctcctatg aagcgcttgc cacctcgctg cagaagctgt
     3121 cgacggatga ggtcaaggtc aatatcatcc ataccggcgt cggtgccatc agcgagtccg
     3181 atgtcaatct gggcgccgct tccaaggctg tcgtcattgg tttcaacgtg cgtgctgatg
     3241 caggcgcgcg taagttgatc gacagttctg gcgtggacgt gcgttactac aacatcatct
     3301 atgaagcggt ggatgaagtg aaagctgcgc tcggcggcat gttgtcgcca gagcagaaag
     3361 aaaacgccat cggtctcgtc gagatccgtg aagtgttccg catctccaag gtcggtgccg
     3421 tggcaggctg ttatgtactg gatggtgtca tcaagcgcgg ctccaaggtc cgtgtattgc
     3481 gcgataacgt cgtcatccac accggtgagc tggattcgct gaagcgcttc aaggatgacg
     3541 tgaaggaagt gaaagccagt ttcgaatgcg gcttgtctct gaagaacttc aacgatatcg
     3601 aagtcggcga tacgctggaa gtgtttgaag tcgtcgaagt ggcgagaaca ctggcataag
     3661 gcatggccaa ggaattctcc cgtagcgacc gcgtagccga gcagatccag cgagagctgg
     3721 ctgatctgct acagttcgag gtcaaggatc cgcgcgtggg catggtgacc atcaccgaag
     3781 tcgaggtgag tggtgacatg tcacacgcca agatctatta cagcgcggca gagggcacca
     3841 aggagctgca gaagggcctg caaaaatccg ctggcttcct gcgtacgcaa ctctccaaac
     3901 gcttgctctt gcgcaccgtg ccacagctgc atttcgtcta cgatgcctcg atcgatcgtg
     3961 gcatgaggct ctccaagctc atcgacgaag cattggcgcc cgaagcgccg accaagtcca
     4021 acaagcactg atcccgccga ttgcagacca aacgtatcaa gcgcgccatt gatggcgtac
     4081 tgctattgga taagccgctg ggcttttcct ctaaccaggc gctgcaaaag gtcaagtggc
     4141 tatatcaggc agccaaggcg ggtcacaccg gtagtctgga tccgctggcc acagggttgc
     4201 tgccgatctg tctgggtgag gcgaccaagt tctcgcattt cctgctggaa gccgacaaaa
     4261 gttaccgtgc gctgattcgg ctcggcagta ccacgaccac cgccgatgcc gaaggcgagg
     4321 tgctaacgcg atccgaagtc agggtcgatg ccagccagtt ggcggacgtc ctgggaagct
     4381 tcgtcggcga catcgtgcag gtgccgccca tgtattcggc cttgaaacac gagggcaaag
     4441 ccctctacga gtacgccagg gctggcgtcg acattcctcg aaagggccgt gaagtcacga
     4501 tccacgagat cgtgctcgag cgtttcgaag gagaaatgct cgaagtggtt gtgcgttgca
     4561 gcaagggcac ctatatccgc acattggccg aggatatcgg ccatgccctg ggttgtgggg
     4621 cacacctggc aggcctgcgg cgattgacga ccgcgcatct cgggcttcag gacgcggtta
     4681 cgatcgaaca gctggaggca ttggccccag cggcaagaga cgccttgtta ttgccggtgg
     4741 acgcgacgat cacagatctg ccgaaggtga c
//
LOCUS       DATMTW010000109         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_9003, whole genome shotgun sequence.
ACCESSION   DATMTW010000109 DATMTW010000000
VERSION     DATMTW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9003"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..>2596)
                     /locus_tag="VN084_02085"
     CDS             complement(218..>2596)
                     /locus_tag="VN084_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28272.1"
                     /translation="SNQLKNLLMILFAQLGLSILLIVGILHSRFLKPMRTLTEQATHL
                     AELHLDTPFLWERKDEIGRLGRHLEWTRAELKRLIDELRAKTLALESDIARRREVEDA
                     LRRSENKYRELFWSNLDGIVISSLDGQVIDANPAFLNLMCYSLDQLKLQNFWSLIAPE
                     SEGMERFNLDNKVLRFGYCDEFEASYINRFGNVVPVSVKTVAMRDSFGRINAVWRMVR
                     DISEKRVAEERMQLAAKVFENTVEGIMITDADKRIRSVNRAFSQITGFGQQEVLGQKP
                     DMLSSGRHDETFYEAMWSAINEHGSWQGELWNRRKNGEVYPEWLAISAVRNAVGEITH
                     YVAIFSDLSERKAADERIQFLAHFDVLTGLPNRAHIQDRVELAIQNATRDNERLALLL
                     LDLDRFKTINESLGHSAGDALLQVLAERIRSTLLTGEVVARQGGDEFIVLLPLIADPG
                     SAALAAERIREVLEPPVELFNHSISITPSIGISVFPDDGRDFETLVRNADAAMYHAKS
                     AGRNSYKFYTADLNARAREILEIESQLRYALDRDEFVLHYQPQVDLKTGEIIGAEALI
                     RWQHPELGLLGPVRFIEVAEERGFIVQIGNWVIQEACRQLAQWRRQGLPRISLAVNLS
                     TLQFRQQDLAVHLQRALSAHGLVGSDLDVEVTESVIMDDVETTIHTIDRIKSMGLQLS
                     IDDFGTGYSSLSYLKRFKADKLKIDRSFVRDIPMDADDSAIARAIINMAKNLNMTVIA
                     EGVETIEQWKFLDREGCHAAQGYLLAKPVPAKEFEKLLEKGILLPERLTSSA"
BASE COUNT          484 a          804 c          788 g          520 t
ORIGIN      
        1 ctttttgtag gtttgcagag ttgatgcaaa ttttcaacgg atagctgaaa aatccagatg
       61 tcggaagagt aaggcccagc cagacatggc tgggcttttt tgtttttaag tccgtcacgg
      121 ctgggttttt atccggttac ccgggaccca tgaataaaaa aggttggccg aagacacgct
      181 tcggccaacc ttggcaagca accgcagagc tgccctatca ggcactggag gtgagtcgct
      241 ccggcagcag gatgcccttc tccagcagct tctcgaactc cttggccggg accggtttcg
      301 ccagcaggta accctgggcg gcatggcagc cctcgcggtc gaggaacttc cactgctcga
      361 tggtctccac cccttccgca atcaccgtca tgttgaggtt cttggccatg ttgatgatgg
      421 cgcgggcaat ggccgaatcg tccgcatcca tcggaatgtc acgcacgaag gaacggtcga
      481 tcttcaactt gtccgccttg aaacgcttaa ggtaggacag actggagtag cctgtaccaa
      541 agtcgtcgat ggaaagctgc agccccatgc tcttgatgcg gtcgatggta tggatggtgg
      601 tttcgacatc gtccatgatc acgctctcgg tcacctcgac gtccagatca ctgcccacca
      661 gcccgtgcgc cgatagtgcc cgctgcaggt gtacggccag gtcctgctgc ctgaactgca
      721 gcgtggacag gttgaccgcc agcgaaatgc gcggcagtcc ctggcggcgc cactgcgcca
      781 gctgacggca ggcctcctgg atcacccagt tgccgatctg gacaatgaag ccccgctcct
      841 ctgcgacctc gatgaagcgt accggcccca gcaggccgag ctcggggtgc tgccagcgga
      901 tcaacgcctc ggcaccgatg atttccccgg tcttcaggtc aacctgcggc tggtagtgca
      961 gcacgaactc gtcgcgatcc agcgcgtagc gcagctggct ctcgatctcg aggatttccc
     1021 gagcccgtgc attgaggtcg gccgtgtaga acttgtagct gttgcggccg gccgatttgg
     1081 cgtggtacat cgcggcatcg gcgttgcgca ccagggtctc gaagtcacgg ccatcgtccg
     1141 ggaacacact gatgccgatc gacggcgtga tcgagatcga gtggttgaac agctcaaccg
     1201 gcggctccag cacctcgcgg atgcgctcgg cggccagtgc cgcgctaccc ggatcggcga
     1261 tcagcggcaa cagcacgatg aactcgtcgc cgccttggcg tgccaccact tcacccgtca
     1321 gcaaggtcga gcggatacgc tctgccagca cctgcagcaa ggcgtcgccc gccgaatggc
     1381 cgagcgactc gttgatggtc ttgaaacggt cgaggtcgag cagcagcagg gccagccgct
     1441 cgttgtcgcg cgtggcgttc tggatcgcca gctcgacccg atcctggata tgcgcgcggt
     1501 tgggcaagcc ggtcagcacg tcgaaatgcg ccaggaactg gatacgctcg tcggcggcct
     1561 tgcgctcgga caggtcgctg aaaatcgcca cgtaatgggt gatttcgccc accgcattgc
     1621 gcacggcgct gattgccagc cactccgggt agacctcacc gttcttgcgg cgattccaca
     1681 actcgccctg ccaggaacca tgctcgttga tggcactcca catcgcttcg tagaaggtct
     1741 cgtcgtgacg gccggaagac agcatgtccg gcttctgccc cagcacttcc tgctggccga
     1801 agccggtgat ctggctgaac gccctgttga cgctgcggat gcgcttgtcc gcgtcggtga
     1861 tcatgatgcc ctcgacggta ttctcgaaca ccttggccgc tagctgcatg cgctcttcag
     1921 ccacgcgctt ttcggagatg tcgcgcacca tgcgccatac cgcgttgatg cgaccgaagg
     1981 aatcccgcat cgccacggtc ttgacgctga ccggcaccac gttgccgaag cggttgatgt
     2041 agctggcctc gaattcatcg caatagccga agcgcagcac cttgttgtcg aggttgaagc
     2101 gctccatccc ctcgctctcc ggcgcgatca gcgaccagaa gttctgcagc ttgagttggt
     2161 cgaggctgta gcacatcagg ttgaggaaag ccgggttggc atcgatcacc tggccgtcga
     2221 gcgagctgat cacgatgccg tctagattgg accaaaacag ctcgcggtac ttgttctcgg
     2281 aacggcgcaa ggcatcctcc acctcgcgcc gccgggcgat gtccgactcc agcgccaggg
     2341 tcttggcgcg caattcgtcg atcaggcgct tgagttcggc tcgcgtccac tcgaggtggc
     2401 gccccagccg gccgatctca tccttgcgct cccagagaaa gggggtgtcg aggtgcaatt
     2461 cggccagatg cgtcgcctgc tcggtcaggg tgcgcatcgg cttgaggaag cgggaatgca
     2521 gaatgccgac aatcagcaga atggacagcc ccagctgggc gaacaggatc atcagcaggt
     2581 tcttgagctg attgct
//
LOCUS       DATMTW010000110         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_42947, whole genome shotgun sequence.
ACCESSION   DATMTW010000110 DATMTW010000000
VERSION     DATMTW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42947"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1514
                     /locus_tag="VN084_02090"
     CDS             90..1514
                     /locus_tag="VN084_02090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWT28273.1"
                     /translation="MSFENLNLHPAILRAIEESGYTTATPIQAQAIPEVMAGHDLMAS
                     AQTGTGKTAAFTLPALNLLATPHPSKSRGPRILVLVPTRELAAQVNDAARKYGKHMRA
                     RCVSIVGGMPYPLQNKLLSQPLDILVATPGRLLDHMERGRIDLSRLQMLILDEADRML
                     DMGFMPDVERICNQLSAERQTLLFSATLEGNIGRIAQQILRNPKTIQVAAQKEKHANI
                     EQRLHFVDDMSHKNKLLEHLLIDPEVNQAIVFTSTKRHADLLAEDLYAAGHKTAALHG
                     DMTQGARNRTLTKLRHGDVKVLVATDVAARGIDVAGISHVINYDLPKFAEDYVHRIGR
                     TGRAGNTVIAVSFASHMDRHQVRKIEQFTGQRIEASVIEGFEPRRPAPRTDRDDRPRG
                     KPGNKGGFKARTQANGPRKDAAGRPFGENKFGGDRKFGDNKPAGARNDSNRGNSAGKR
                     SDDRFAGEKRSFGDRAAKRPRTFA"
     gene            1515..>1674
                     /locus_tag="VN084_02095"
     CDS             1515..>1674
                     /locus_tag="VN084_02095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWT28274.1"
                     /translation="MNTVTTENTSETVRFDALGLAPELLKALTDSGYTTPTPIQAQAI
                     PLVLEGRDL"
BASE COUNT          367 a          554 c          456 g          297 t
ORIGIN      
        1 gttttgcgca tcgctttcaa cacctcaagg tggatgaacg ccatgtgtct gcttgcgccc
       61 ttttagcttt atcctcgaaa gggaaatttg tgtcttttga aaatctgaat ttacatccag
      121 caatcctgcg tgccattgaa gaatctggct acaccaccgc tacgcccatc caggcccagg
      181 cgatcccaga agtcatggct ggacacgacc tcatggcctc cgcgcagacc ggcaccggca
      241 agaccgctgc gttcacgctg ccggcactga acctgctggc aacgccacac ccgtccaaga
      301 gccgtggccc acgcatcctg gtactggtac cgacgcgtga actggctgcg caagtcaatg
      361 atgccgcccg caagtacggc aagcacatgc gcgcccgctg cgtgagtatc gtcggcggca
      421 tgccttaccc actgcagaac aagctgctga gccagccact ggatatcctg gtcgctacgc
      481 ctggccgtct gctggaccat atggaacgcg gtcgtatcga cctctcccgc ctgcagatgc
      541 tgatcctgga cgaagccgac cgtatgctgg acatgggctt catgccggat gtcgagcgta
      601 tctgcaacca gttgtccgca gaacgccaga ccctgttatt ctccgccacc ctcgaaggca
      661 acatcggccg catcgcgcag cagatcctgc gcaatccgaa gaccatccag gtggccgctc
      721 aaaaagagaa gcatgccaat atcgaacagc gtctgcattt cgtcgacgac atgtcgcaca
      781 agaacaagct gctggaacat ctgctgatcg atccggaagt gaaccaggcc atcgtcttca
      841 cctcgaccaa gcgtcacgcc gacctgctgg cggaagacct gtacgctgcc ggccacaaga
      901 ccgcagccct gcatggcgac atgacccaag gtgcccgtaa ccgtacgctg accaagctgc
      961 gtcacggtga tgtcaaggta ttggtagcga ctgatgtcgc cgcgcgcggc atcgacgtcg
     1021 ccggcatctc gcacgtcatc aattacgatc tgccgaaatt cgccgaagac tatgtccacc
     1081 gcatcggccg caccggccgt gccggcaaca cggtgatcgc ggtctccttc gcttcccata
     1141 tggacaggca ccaagtccgc aagatcgaac agttcaccgg ccagcgcatc gaagccagcg
     1201 tgatcgaagg cttcgagcct cgtcgccctg cccctcgtac cgatcgtgat gatcgtccac
     1261 gcggcaagcc aggcaacaag ggcggcttca aggctcgcac ccaggccaat ggcccccgca
     1321 aggacgctgc aggcaggccg ttcggtgaga acaagttcgg tggcgaccgc aagttcggcg
     1381 acaacaagcc ggctggggca cgcaacgata gcaaccgcgg caattccgca ggtaaacgca
     1441 gcgatgatcg ctttgccggc gagaagcgca gctttggcga ccgcgccgca aaacgtccac
     1501 gcacctttgc ctaaatgaat accgtcacta ctgaaaatac cagcgaaacc gtccgtttcg
     1561 acgcactcgg tctggcgcct gaactgctga aggcgctgac cgattccggt tacacgaccc
     1621 cgacgccgat ccaggcccag gcgatcccgt tggtgctgga agggcgcgac ctca
//
LOCUS       DATMTW010000111         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_210817, whole genome shotgun sequence.
ACCESSION   DATMTW010000111 DATMTW010000000
VERSION     DATMTW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210817"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="adk"
                     /locus_tag="VN084_02100"
     CDS             complement(<1..570)
                     /gene="adk"
                     /locus_tag="VN084_02100"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWT28275.1"
                     /translation="MRIILLGAPGAGKGTQATFIKEKFNIPQISTGDMLRAAVKAGTQ
                     LGLEAKKFMDAGGLVPDEVIIGLVKERIKDEDCKSGFLFDGFPRTIPQAEAMKQAGVG
                     IDYVVEIDVPDEEIVKRMSGRRAHLASGRTYHVVFNPPKVAGKDDVTGEDLVQRDDDR
                     EEVVQKRLEVYHAQTEQLVGYYSDWASSGI"
     gene            complement(600..1400)
                     /gene="rsmA"
                     /locus_tag="VN084_02105"
     CDS             complement(600..1400)
                     /gene="rsmA"
                     /locus_tag="VN084_02105"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWT28276.1"
                     /translation="MKHVAKKRFGQNFLNDQAIITSLVDAIAPRHDDLMVEIGPGLGA
                     LTQPLLKKLDHLHVVEIDRDIISWMKNQYPADKITIHERDALKFDFSQLQSGSLAEVK
                     RIRVVGNLPYNISTPILFHLLDNVGSIVDMHFMLQKEVVERMVAEPSNAAYGRLSVML
                     QYRLQMEYLFTVPPEAFDPAPKVESAFVRVVPHAVLPHPADDVAMLARVVTAAFGQRR
                     KTLRNTLKGLLDDAGFATLEIDPQLRAENLSVAEFVAISNHLSRTQPA"
     gene            complement(1400..2392)
                     /gene="pdxA"
                     /locus_tag="VN084_02110"
     CDS             complement(1400..2392)
                     /gene="pdxA"
                     /locus_tag="VN084_02110"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWT28277.1"
                     /translation="MLKLPRIAVTAGEPAGIGLDLCVMLARHPFPAALVVIANRDALL
                     ARAAQLGQVIECLNYDAQHCVPHAGDGRLTVLDIHTATPPVAGKLDTANGPHVLETLQ
                     RAIDGCVSGEFDAMTTAPVHKGVINDAGVAFTGHTEFLAEQTHTPEVVMMLVGAGMRV
                     ALVTRHLPLTQVSAAITQPSLDRTIRILAHDLVHHFGIAEPRILVAGLNPHAGEGGYL
                     GTEEITTITPVLERLRAEGMTLLGPLPADTLFTPHNLEKGDCVLAMYHDQGLPVLKHA
                     SFGGGVNVTLGLPIIRTSVDHGTALDIAGKGIADIGSLLAAIELAIELANSRKH"
     gene            complement(2411..>2704)
                     /locus_tag="VN084_02115"
     CDS             complement(2411..>2704)
                     /locus_tag="VN084_02115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWT28278.1"
                     /translation="AANGGDLGWVNPGDTVPQFEKAMNALQPGEISQPIRSPFGWHII
                     QVLERRKQDMTKEAARLKARQEIRARKSEEAYQDWVRELRDRAYVELRLEDKY"
BASE COUNT          479 a          791 c          861 g          573 t
ORIGIN      
        1 gataccggag ctcgcccagt cagagtagta gccgaccagt tgctcggtct gggcgtggta
       61 gacctcaaga cgtttctgca cgacttcctc gcggtcatca tcacgctgga ccaggtcttc
      121 gccggtgacg tcgtccttgc ctgcgacctt gggcgggttg aacaccacat ggtaggtgcg
      181 cccggaggcc agatgcgcgc ggcgaccgct catgcgtttg acgatctctt cgtcgggcac
      241 gtcgatctcg accacgtaat cgatgccgac accggcctgc ttcatggctt cagcttgcgg
      301 aatggtgcgc gggaagccgt cgaagaggaa acctgacttg cagtcctcgt ccttgatgcg
      361 ttccttgacc agcccgatga tgacttcgtc cggtaccagg ccgccggcat ccatgaactt
      421 cttggcttcc agccccaact gggtgccggc cttgaccgca gcacgcagca tgtcgccggt
      481 ggagatctgc gggatgttga atttctcttt gatgaacgtg gcttgcgtgc ccttaccggc
      541 gccgggtgcg cctaacaaaa tgattctcat gcggttcctc ccttggaaac aggattatat
      601 caggcgggct gtgtgcggct aaggtgattg ctgatggcga cgaactcggc cacgctcaga
      661 ttctcggcgc gcagttgcgg gtcgatctcc aaggtcgcga aaccggcgtc atccagcagg
      721 cccttgagcg tgttgcgcag ggtcttgcgc cgctggccga aagccgccgt gacgaccctg
      781 gcgagcatgg ctacatcgtc agcggggtgg ggcaatacgg catgcggcac cactcgcacg
      841 aaggctgact cgaccttggg cgcagggtca aaggcctcgg gcggtacggt gaagaggtat
      901 tccatctgca gacggtattg cagcatgacc gaaagccggc cgtaagcggc attcgacggc
      961 tctgccacca tgcgttcgac cacttccttt tgcagcatga aatgcatatc gacgatgctg
     1021 ccgacgttat ccagcaggtg gaacaggatg ggtgtggaga tgttgtaagg caggttgccc
     1081 accacacgga tccgcttcac ctccgctagc gagccagatt gaagttgcga gaaatcgaat
     1141 ttcaacgcgt cgcgctcgtg gatggtgatc ttgtctgccg gatactggtt tttcatccag
     1201 ctgatgatgt cacggtcgat ctcgaccacg tgcagatgat ctagcttctt cagcaggggc
     1261 tgcgtcaatg cgccgagtcc cggcccgatc tcgaccatga ggtcgtcatg ccggggggcg
     1321 atggcatcga ccaggctagt gatgatggcc tggtcgttga ggaagttctg gccgaatctt
     1381 ttctttgcta cgtgtttcat cagtgttttc ggctgttcgc cagctcgatg gcgagttcga
     1441 tagccgccag caggctaccg atgtcggcga tgcctttgcc ggcgatatca agcgcggtgc
     1501 catggtcgac cgaggtgcgg ataatcggta aacccagcgt cacattgacg ccgccaccga
     1561 aactcgcatg cttcagtacg ggcaggccct ggtcatggta catggccagc acgcaatcac
     1621 ccttctcaag gttgtgcggt gtgaagagag tatcggcagg cagcggccca agcaacgtca
     1681 tgccttctgc gcgcagcctt tccaacactg gcgtgatggt ggtgatctcc tctgtaccca
     1741 ggtagccccc ttcgccggca tgcggattga gcccggcgac caggatgcgc ggctcggcga
     1801 tgccgaaatg gtgcacgaga tcatgcgcca ggatgcggat ggtgcgatcg aggctaggct
     1861 gggtgatggc ggcgctgacc tgcgtcagcg gcaagtggcg cgtgacaagc gctacccgca
     1921 tgccggcacc aaccagcatc atgacgactt caggcgtatg ggtctgttcc gcgaggaatt
     1981 ccgtgtggcc ggtgaaggcg acaccggcat cgttgatgac gcctttgtgg actggcgccg
     2041 tcgtcatggc gtcgaattcg ccgctgacgc agccgtcgat cgcccgctgc agggtctcta
     2101 gcacatgagg gccattggcc gtgtcgagct tgccggccac tggtggcgtg gcggtatgga
     2161 tatccaggac cgtcagcctg ccgtcacccg cgtgtggcac gcagtgctgc gcatcgtagt
     2221 tcagacattc gatcacttgc ccgagctgtg ccgcgcgcgc cagcagggcg tcgcggttgg
     2281 cgatgacgac cagcgctgcc gggaaagggt gtcgcgccag catcacgcac agatcgagac
     2341 cgatgccggc aggctcaccg gcagtgacgg caattcgggg cagcttgagc atatggcggg
     2401 gtgaagcggc ttagtatttg tcttcgagac gcagttcgac gtaggcacgg tcacgcagtt
     2461 cgcgcaccca gtcctgatag gcctcttccg atttgcgtgc gcggatctcc tgcctggcct
     2521 tgagtcgtgc tgcttccttc gtcatgtcct gcttgcgacg ctccaggacc tggatgatat
     2581 gccagccgaa cgggctgcgg atcggttggc tgatctcacc tggctgcagc gcattcatgg
     2641 ctttctcgaa ttgcgggaca gtatcgcctg gattgaccca gccgagatcg ccgccattgg
     2701 cggc
//
LOCUS       DATMTW010000112         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_445459, whole genome shotgun sequence.
ACCESSION   DATMTW010000112 DATMTW010000000
VERSION     DATMTW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445459"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VN084_02120"
     CDS             <1..1273
                     /locus_tag="VN084_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28279.1"
                     /translation="ARLLGGLRAAFLAALLLSITGAWSGAMFTHTKDVPFGACMAWAL
                     YYTVLISQQLPRFPRALAIKLGIAVGCALGLRIGAAFAVIYLLLMVMLAAWCQRDGWR
                     QRCDFIAATVVSLLPAGIVAFILMAVFWPWGVMSPSHPLEAAKAFSHFTFNMLTIMDG
                     EVMNIGSVPRAYLPAYLLVRLPEAFLMGLASLLIFNWCRIRKASHADILAWFSVSIAV
                     VFPLAFILFDKPALYNGIRHFTFLLPPLAILAGLGLSLAWDRLVDRPPLRLGFAALFA
                     AVCLLNTVTLVRLHPYEYVYYNHLAGSLPKAEHEWEGDYWSSSLREAADKLEALIPGI
                     NKADPRADYLVAVCAESIQGDAYLDDRFEITRDWMAADFYISTTNMNCDKVMQGKIIA
                     TVERMGTVLAVVKDRRALNGHLRRPRPVPKP"
     gene            1288..2259
                     /locus_tag="VN084_02125"
     CDS             1288..2259
                     /locus_tag="VN084_02125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWT28280.1"
                     /translation="MEDRSAASPHITVIVPAYNEARAISATLASIAAQLGQLSPEWNI
                     VVVDDGSRDETAEVVKGLPASLHVTLVRFSRNFGKEYAITAGLEYAKGDVVICMDADG
                     QHSSELLSEMLAKWRLGYDMVYAVRNDRGAESRFKRWGSRLFYKAISLGGQIHIPRDA
                     GDFRLMHRNVVDALLALPERNRFMKGMYAWVGYKSIGIPFTPLARAHGESAFSRLKLI
                     QLAWTGVTSFSVMPLRLASAVGMILALLAFCYGLLVVIDKLFFHESVPGWPTVVASIM
                     FFSGVQLLFIGILGEYLARIYDEVKGRPPYIVAEVVEPAAVSAQAHG"
     gene            2264..2647
                     /locus_tag="VN084_02130"
     CDS             2264..2647
                     /locus_tag="VN084_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWT28281.1"
                     /translation="MSLQRSLSWFTLIGAIAAAVHYVVAVTLEGGLHVAPAWANVTGF
                     VFAFPVSYAGHSRLSFAHHAASHRQALPRFLAIALTGFASNQVLLLSALAWLDWPFWL
                     VLALVMGIVACLTYLLSRYWAFRAA"
     gene            2644..3456
                     /locus_tag="VN084_02135"
     CDS             2644..3456
                     /locus_tag="VN084_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWT28282.1"
                     /translation="MTPLIIAADDYAQSAAIDQGILALVARSRLTAFSCLVLSPRWPA
                     AARQITPEVRSKADIGLHLDFTQYAQNAPAALPWLIVKSTLRSLSHKAVRAAIMTQCN
                     RFEDALGTMPDYVDGHQHVHQLPQIREALIDVLTQRYQHRLPWLRIARPPAASGFKAA
                     IIGALGSSALAQLARQNGIRHTSALLGVYDFKGNTSEYGARLAGWLAQASRSKPCCAL
                     MCHPAMEIGVSADDLQDPIHAARLNEYRIFANDRFPSLMATYAVEPVRGDQL"
     gene            complement(3485..>3732)
                     /locus_tag="VN084_02140"
     CDS             complement(3485..>3732)
                     /locus_tag="VN084_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28283.1"
                     /translation="YAGQAIADSNRLSLTLQATDRRSADNSIGYATMKKDSHGHWTFR
                     KNYYEPNDNGGVNGTEFCVLKISLVNPNRPKNAKPEF"
BASE COUNT          641 a         1197 c         1128 g          766 t
ORIGIN      
        1 ggcacgactg cttggcggcc tgcgcgcggc ctttctcgcg gctttactgc tgagcatcac
       61 cggtgcctgg tcaggcgcca tgttcacgca caccaaggac gtccccttcg gcgcatgtat
      121 ggcctgggcc ctctactaca ccgtgctgat ctcgcaacag ctgccgcgat ttccgcgtgc
      181 gctcgcgatc aagctcggca tcgctgtcgg ttgtgcgcta ggcctgcgca tcggcgcggc
      241 attcgcggtc atctatctgc tgctgatggt catgctggct gcatggtgcc aacgcgacgg
      301 ctggcgtcag cgttgcgact tcatcgccgc taccgtggta tcgttgctgc cggcaggcat
      361 cgtcgcgttt atcctcatgg cggtattctg gccctggggc gtgatgtcgc cgagccaccc
      421 gctggaagcg gccaaggcat tctcccattt cactttcaac atgctgacca ttatggacgg
      481 cgaggtcatg aacatcggca gcgtgccccg cgcctacctg cccgcctacc tgctggtgcg
      541 cctgccggag gcttttctca tgggcctggc gagcctgctg atctttaact ggtgccggat
      601 ccgcaaggcc agccatgcgg atatcctggc ctggttcagc gtttcgatcg cggtggtgtt
      661 cccactggcg ttcatcctgt tcgacaaacc cgcgctatat aacggcattc gccatttcac
      721 cttcctgctg ccgcccctgg cgatcctggc cggccttggc ctgagcctgg cctgggacag
      781 gctggtcgac cggccaccgc tacgcctggg gttcgctgcg ctgttcgcgg cggtgtgcct
      841 gctgaacacg gtgaccctgg tcaggctgca tccttatgaa tatgtgtatt acaaccatct
      901 ggccggtagc ctgccaaaag cggaacacga atgggagggc gactactggt cgagcagcct
      961 gcgcgaggca gccgacaaac tggaggcctt gatccccggc atcaacaagg cggacccacg
     1021 cgccgactat ctggtcgccg tctgtgccga gagcatccag ggcgacgcct atctggatga
     1081 ccgtttcgag atcacgcgcg attggatggc tgccgatttc tacatcagca cgaccaacat
     1141 gaattgcgat aaagtcatgc aaggcaagat catcgccacg gtggaacgca tggggaccgt
     1201 actggctgtc gtcaaggaca ggcgcgcgct caacggccac cttcgccggc cacgcccggt
     1261 acccaaaccc taatccggag cactcgcttg gaagaccgca gcgccgcaag ccctcatatc
     1321 acggtcatcg tccccgccta taacgaagcg cgggcgatct cggccacact cgccagtatc
     1381 gcagcgcaac tgggccaact gagccccgaa tggaacatcg tcgtcgtcga cgacggcagc
     1441 cgcgacgaga cggccgaggt ggtcaagggc ctgccagcat cgctgcatgt cacactggtg
     1501 cggttctcgc gcaacttcgg caaggaatac gcgatcaccg ccgggctgga gtatgccaag
     1561 ggtgacgtgg tcatctgcat ggatgcggac ggtcagcact cgagcgaact gctaagtgag
     1621 atgctggcga aatggcggct gggctatgac atggtctacg ccgttcgcaa tgaccgcggc
     1681 gccgaatcgc gcttcaagcg ctggggctct cgcctcttct acaaggccat cagtctcggc
     1741 gggcagatcc atatcccgcg cgatgccggt gattttcgct tgatgcatcg caatgtggtc
     1801 gatgccctgc tcgcgttgcc ggaacgaaat cgcttcatga aaggcatgta tgcctgggtc
     1861 ggctacaagt cgatcggcat ccccttcacg ccgctggcgc gagcgcatgg cgagagcgct
     1921 ttctcccgcc tgaagctgat ccagctggcc tggaccgggg tcaccagctt ttcggtgatg
     1981 ccgttgcgac tggccagcgc ggtcggcatg atcctcgcgc tgctggcctt ctgctacggg
     2041 ctactggtgg tgatcgataa gctgttcttc cacgaatccg tacctggctg gccgacggtg
     2101 gtcgccagca tcatgttctt ctctggcgtg cagctgctgt tcatcgggat actgggtgaa
     2161 tacctggcgc gcatctacga cgaagtcaaa ggtcgcccgc cctatatcgt ggccgaagtg
     2221 gtcgaacccg cagcggtgtc ggcacaagcg cacggataag tccttgagcc tgcaacgctc
     2281 cctctcctgg ttcaccctga tcggcgcgat cgccgcagcc gtgcattacg tggtggcggt
     2341 gacgctggag ggcggcctgc acgttgcacc ggcctgggcc aatgtcactg gcttcgtgtt
     2401 tgcgttcccg gtgagttatg ccggccacag caggctctcg ttcgcgcatc acgccgcctc
     2461 gcatcgccag gcgttgccgc gtttcctggc catcgcgctg accggcttcg ccagcaacca
     2521 ggtgctgctg ctgagtgccc tcgcctggct ggactggccg ttctggctgg tgctggccct
     2581 tgttatgggt atcgttgcct gcctaactta tttgctcagc cgctattggg ctttccgcgc
     2641 cgcatgacgc cgctgatcat cgccgccgac gattatgcgc aaagcgcagc catcgaccag
     2701 gggatcctgg cgctggtcgc gcgttcccgc ctcactgcgt tctcctgcct ggtgttgagc
     2761 ccgcgctggc cggccgcagc ccgtcagatc acaccagaag ttcgcagcaa agccgatatc
     2821 ggcctgcatc tggatttcac ccagtacgcg caaaacgcgc ctgcagcgct gccttggcta
     2881 atagtcaagt cgacgttgcg cagcctgtca cacaaggccg tacgcgctgc catcatgacc
     2941 caatgcaatc gattcgagga cgcgctcggc acgatgccgg attacgtcga cggccaccag
     3001 catgtccacc agctgcccca gatccgcgag gcgctgatcg atgtgctgac gcaacgttac
     3061 cagcatcgcc tgccatggct tcggatcgcc agaccgccag ccgcttcagg cttcaaggcc
     3121 gccatcatcg gcgcgctcgg cagcagcgca ttggcccaac tggcgcggca gaacggtatc
     3181 cggcatacca gcgccctgct cggggtctat gacttcaagg gaaatacttc ggaatacgga
     3241 gcacgactgg ccggctggct ggcgcaggcc tcacgatcga aaccctgctg cgccctgatg
     3301 tgccacccgg ccatggagat cggggtcagc gcagacgatc tgcaggatcc gatccatgcc
     3361 gcacggctga atgaatatcg tatcttcgcc aatgaccggt tccccagcct catggcgacc
     3421 tatgcggtag aaccggtacg gggcgatcag ctgtaaaaaa acccgccgaa gcgggtgatt
     3481 gcggtcagaa ctccggcttg gcgttcttcg gccggttagg attgaccagg ctgatcttca
     3541 acacgcagaa ctcggtgcca ttgacaccac cgttatcgtt aggctcgtag tagttcttgc
     3601 ggaaggtcca gtgcccatgg ctgtccttct tcatggtggc ataaccgatg ctgttgtcgg
     3661 cactgcgtcg gtcagtcgcc tgcaaggtca gcgaaaggcg gttgctgtcg gcgatcgctt
     3721 ggccggcgta gg
//
LOCUS       DATMTW010000113         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_110801, whole genome shotgun sequence.
ACCESSION   DATMTW010000113 DATMTW010000000
VERSION     DATMTW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110801"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VN084_02145"
     CDS             complement(<1..200)
                     /locus_tag="VN084_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWT28284.1"
                     /translation="MLEKLIAALAVWIIGVISTLGYGGIVLLMAIESACIPLPSEIIM
                     PFAGFLVFKGELLLWGVALAGAA"
     gene            complement(200..>3031)
                     /gene="mfd"
                     /locus_tag="VN084_02150"
     CDS             complement(200..>3031)
                     /gene="mfd"
                     /locus_tag="VN084_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWT28285.1"
                     /translation="ESIRTFDVDTQRSLYPVGEIRLLPAREFPLDDKGISLFRQQFRE
                     TFEGDPSRSRIYKDVSKGIASGGIEWYLPLFFEETATLFDYLPQDAVLCMHGDLDQAA
                     QVFLAETQSRYRLLAHDPERPILKPEQLLIKADEFFAQTHAYARIALAPDASAATLPA
                     LDIDRRAEQPLHKLQAFLKSHKGRALILAESLGRRETIGQLLADNGLQPSLTESWEAF
                     IASDAPFVLGIAPLHKGFVSDKVAVITEAELYAATVRQQRRRDKEKARSTEGMLKDLS
                     ELRENDPVVHEQHGVGRYRGLVNLDFGEGETEFLLLEYAGDDKLYVPVSQLFLISRYS
                     GGPPESAPLHRLGSGHWEKAKKKALKQIRDTAAELLNLYAQRAARKGHAFKLTLQDYE
                     AFAEGFPFEETPDQLAAIEAVISDMQSGRPMDRLVCGDVGFGKTEVALRAAFVAVMGG
                     RQVAVLVPTTLLAEQHYNNFTDRFAEWPIKIAEISRFRTAKEQAEAIRGLQTGEIDII
                     IGTHRLIQKDVKFKNLGLVILDEEHRFGVRQKEQLKALRAEVDVLTLTATPIPRTLSM
                     AMEGLREFSVIATPPQKRLAIKTFHADYSEGIIREAAMREFKRGGQVYFLHNEVDTIH
                     AMREKLERILPEARIGIAHGQLRERELEHVMRDFYHQRFNLLLCTTIIETGIDVPTAN
                     TIIMNKADMFGLAQLHQLRGRVGRSHHQAYAYLLTDPDRNITPQAQKRLDAIQLLEDL
                     GAGFHLAMHDLEIRGAGELLGDSQSGEMQEIGFSLYADMLNHAVKQLKAGKEPDINAP
                     LGVTTEINLHTPALLPNDYCPDVHERLVLYKRLANCTNDDELDTLQEELIDRFGLLPE
                     QGEALMACHRLRVAAKPLDILKIDASDSAIQLQFNPKADLDPAKLIGLLQRDRRCRMN
                     GPDKLRVTVQLHDINHRADFIKVLLKEFV"
BASE COUNT          536 a          898 c          988 g          609 t
ORIGIN      
        1 gccgcgccgg ccagcgccac accccacagt agcagctcgc ccttgaacac gagaaatccg
       61 gcgaacggca tgatgatctc ggagggcagg gggatgcagg cggattcgat cgccatcagc
      121 agcacgatgc cgccatagcc cagggtggag atgacgccga tgatccatac ggcgagtgcg
      181 gcgatgagtt tttccagcat cagacgaatt ccttgagcaa gaccttgatg aagtcggcgc
      241 ggtggttgat gtcatgcagc tgcaccgtca cgcgtaattt gtcaggccca ttcatacggc
      301 aacggcggtc gcgctgcaat agcccgatca gcttggctgg gtcgaggtcg gccttgggat
      361 tgaattgcag ctggatggcg ctatcactcg cgtcgatctt gaggatatcc agcggcttgg
      421 cggcgacgcg caggcgatgg caggccatga gcgcctcacc ctgttccggc agcaggccga
      481 agcggtcgat cagttcttcc tgcagggtat ccagctcatc gtcgttcgtg cagttcgcca
      541 ggcgcttgta gagcaccagc cgctcgtgca cgtccgggca gtagtcgttt ggcagcaacg
      601 ccggcgtatg caggttgatc tcggtggtga cgcccagcgg cgcgttgatg tccggctcct
      661 tgccggcctt gagctgtttg acggcgtgat tgagcatgtc ggcatagagc gagaagccga
      721 tctcctgcat ctcgccggat tgcgaatccc ccagcagttc gcccgcaccg cggatctcga
      781 gatcgtgcat cgccaagtga aagcccgcgc ccaggtcttc cagcagctgg atggcgtcca
      841 ggcgtttctg cgcctgtggc gtgatgttgc ggtcagggtc ggtcaacagg taggcatagg
      901 cctggtgatg cgaacggcca acgcggccgc gtagctggtg cagctgcgcc aggccgaaca
      961 tatcggcttt gttcatgatg atggtgttgg cggtcggcac gtcgatgccg gtttcgatga
     1021 tggtggtgca cagcagcaaa ttgaagcgct ggtgatagaa atcccgcatc acatgctcca
     1081 gctcgcgctc acgcaactgg ccatgggcga tgccgatgcg cgcttcgggg aggatacgct
     1141 ccagcttttc gcgcatggcg tggatggtat ccacctcgtt gtgcaggaaa tacacctgac
     1201 cgccgcgctt gaattcccgc atcgccgctt cgcggatgat gccttcggag taatcggcat
     1261 ggaaggtctt gatcgccagg cgcttctgcg gcggcgtggc gatcaccgag aattcgcgca
     1321 ggccttccat cgccatcgac aaggtacgcg ggatcggcgt cgccgtcagc gtcagtacat
     1381 caacctctgc gcgcaaggcc ttgagctgct ccttctggcg cacgccgaaa cgatgttcct
     1441 cgtcgaggat gaccaggccc aggttcttga acttgacgtc cttctggatc aggcgatgcg
     1501 tgccgatgat gatatcgatc tcgcccgtct gcaggccgcg gatggcttct gcctgctcct
     1561 tggcggtgcg gaaccgcgag atctcggcga tcttgatcgg ccactcggcg aagcggtcgg
     1621 tgaagttgtt gtagtgttgc tcggcgagca gtgtggtcgg caccagtacg gccacctggc
     1681 gtccgcccat cacggcgacg aacgccgcac gcagggcgac ctcggtcttg ccgaagccga
     1741 catcgccgca gaccagccgg tccattggcc ggcccgattg catatcgctg atgacggcct
     1801 caatggctgc cagctggtct ggcgtctcct caaacgggaa gccttcggcg aaggcttcgt
     1861 aatcctgcag cgtgagcttg aacgcatggc ccttgcgcgc ggcgcgctgg gcatagagat
     1921 tgagcagttc ggcggcggtg tcgcggatct gcttcagtgc cttcttcttg gctttttccc
     1981 agtggccgct gcccaggcga tgcagcgggg cggattccgg cgggccaccc gaatagcgcg
     2041 agatgaggaa cagctgcgag actggcacat agagcttgtc gtcaccggcg tattccagca
     2101 gcaggaactc ggtctcgcct tcgccgaaat ccaggttgac cagcccgcgg taacgcccga
     2161 cgccatgctg ctcgtggacc accgggtcgt tctcgcgcag ctcggagagg tccttgagca
     2221 taccctcggt gctgcgcgct ttttccttgt cgcggcggcg ctgctggcgc acggtcgctg
     2281 catagagttc ggcctcggtg atcacggcga ccttgtcact gacgaagccc ttgtgcaacg
     2341 gcgcgatgcc cagtacgaaa ggcgcatcgc tggcgatgaa tgcttcccag gattcggtca
     2401 gtgacggttg cagtccattg tcggccagca gctggccgat ggtctcgcgc ctgcccaggc
     2461 tttccgccaa gatcaacgca cgacccttat gggatttgag gaatgcctgc agcttgtgca
     2521 gcggttgttc cgcccggcgg tcgatatcca acgcaggcag ggtcgccgca ctggcgtcag
     2581 gcgcaagtgc gatacgcgca taggcatggg tctgcgcgaa gaattcgtca gctttgatca
     2641 ggagctgttc aggcttgagg atcggccgtt ccgggtcatg cgccagcagg cggtagcgtg
     2701 actgggtctc ggcaaggaac acctgggccg cctgatcgag atcgccgtgc atgcagagca
     2761 cggcgtcttg cggcaggtaa tcgaacaagg tcgcggtctc ttcgaagaaa agcggcagat
     2821 accattcgat gccgccactg gcgatgccct tgctgacgtc cttgtagatg cggctacgcg
     2881 aagggtcgcc ctcgaaggtt tcgcggaact gctggcggaa caaggagatg cccttgtcgt
     2941 ccagcgggaa ttcacgggca ggcagcaaac ggatctcgcc gacaggatag aggctgcgct
     3001 gggtgtcaac atcgaaggta cggatgctct c
//
LOCUS       DATMTW010000114         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_412197, whole genome shotgun sequence.
ACCESSION   DATMTW010000114 DATMTW010000000
VERSION     DATMTW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412197"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1387)
                     /locus_tag="VN084_02155"
     CDS             complement(161..1387)
                     /locus_tag="VN084_02155"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWT28286.1"
                     /translation="MALIVQKYGGTSVANPERIQNVARRVAHYKALGHQVVVVVSAMS
                     GETNRLIGLAKEIMPTPDPRELDQMVATGEQVTIALTAMALMDLGLKAKSYTGGQVRI
                     LTDNAHTKARILDIDGSNMRKDLDAGYVVVVAGFQGVDELGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRLKSITFEEMLELASQGSKVLQIRSVEF
                     AGKYKVKLRVLSSFEEEGEGTLITFEEEDNMEQPIISGIAFNRDEAKITVLGVPDRPG
                     IAYQILGPIADANIDVDIIIQNTGADGTTDFTFTVHKNELNKALDILRNQVQKHIGAR
                     EITGDDKIAKVSIVGVGMRSHVGIASQMFRTLAEEGINIQMISTSEIKIAVVIDEKYM
                     ELAVRVLHKAFELDQA"
     gene            complement(1440..>4038)
                     /gene="alaS"
                     /locus_tag="VN084_02160"
     CDS             complement(1440..>4038)
                     /gene="alaS"
                     /locus_tag="VN084_02160"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWT28287.1"
                     /translation="PTLLFTNAGMVQFKNVFTGQEQRSYKRATSSQKCVRAGGKHNDL
                     DNVGYTARHHTFFEMLGNFSFGNYFKDRAIELAWNLITKEFALPQERLLVTVYAEDDE
                     AYDIWHKEIGIPAEKIVRIGDNKGARYASDNFWMMGDTGPCGPCSEIFYDHGAHIPGG
                     PPGSPDEDGDRFIEIWNNVFMQFNRDEAGVMHKLPKPSVDTGMGLERISAVLQGVHAN
                     YEIDLFQTLIKAAAREVTNALSTEAIKFRSGLMAGSNGEMMPIDIDSPSLKVLADHIR
                     ACSFLIADGVIPGNEGRGYVLRRIIRRAIRHGYKLGARAAFFHRMVPDLVAEMGDAYP
                     ELASGQKRIMDVLKQEEDRFFETIENGMAILETELAEMANSGNATFNGETAFKLHDTY
                     GFPLDLTADICRERGVTVDAAAFDAAMARQKEQARAAGKFKMAANLEYAGKATTFHGY
                     DTLEAKGTVLALYKDGAAVQQLDEGDMGVVVLDDTPFYAESGGQVGDRGELKAADGIF
                     AVEDTQKIQASVFGHHGTLKTGTLKVGDAVAAKVDLVARGRTMRNHSATHLMHKALRE
                     VLGEHVQQKGSLVDSEKTRFDFVHNAPMTDAEITEVERRVNAEIIANAPTLARVMDIE
                     SAQKTGAMMLFGEKYGDEVRVLDIGTSRELCGGTHVQRTGDIGLFKIYAESGVAAGVR
                     RVEATTGDVALAYVQQQQALLNDAASAFKAPAQELPGKIGQMIDNVKALEKELDRLKS
                     KMAASQGDDLASQAVDIAGIKVLAAKLEGVDAKGLREVADKLKDKLGHCALLLAVTEG
                     DKVSLVAAVSKDLTTRIKAGDLVNQVATQVGGKGGGRPDMAMAGGTDASKLPQALASV
                     NDWVRQQLG"
BASE COUNT          678 a         1190 c         1255 g          915 t
ORIGIN      
        1 gaaccctcga tacaggttta agcccgtatg cttccttagc agggaagtgc cttcgaccac
       61 tcggccagct ctccacacga gggcgtaacg atacgcgaat acgccttaaa aatcaaacca
      121 aatcggcccg ctccaggctt cccttatggc cctggattgg ctaagcctga tcgagttcga
      181 acgccttgtg caatacgcgt accgcgagtt ccatgtactt ctcgtcgatg acgacggcga
      241 tcttgatctc ggaggtggag atcatctgga tgttgatgcc ttcttccgcc agcgtgcgga
      301 acatctggct cgcgatgccg acgtgggagc gcatgccgac gccgacgatg gagaccttgg
      361 cgatcttgtc gtcaccggtg atctcgcgtg cgccgatgtg cttctgtacc tggttgcgca
      421 ggatatcaag cgccttgttg agctcattct tgtgcacggt gaaggtgaaa tcggtggtgc
      481 catcagcccc ggtgttctgg atgatgatgt cgacatcgat gttcgcgtcg gcgatcgggc
      541 ccaggatctg gtaggcgata ccgggacggt ccggcacgcc aagcacggtg atcttggctt
      601 cgtcgcggtt gaaggcgatg ccggagatga tgggttgttc catgttgtct tcttcctcaa
      661 atgtaatcag cgtgccttcg ccttcttcct cgaagctgga tagcacgcgc agtttgacct
      721 tatatttgcc ggcgaactcg accgaacgga tctgcagcac cttggagccc tgactggcca
      781 attccagcat ctcttcgaag gtgatggatt tcaggcggcg cgcttccggt acgacacgcg
      841 ggtcggtggt gtagacgccg tcgacatcgg tgtagatctg gcattcgtcg gccttgagcg
      901 cggcagccag ggcaacgccc gtggtgtccg atccgccgcg tcccagcgtg gtgatgttgc
      961 ccaattcgtc cacgccctgg aagccggcga cgacgacgac ataacctgcg tcgagatcct
     1021 tacgcatgtt gctgccgtcg atatcgagga tgcgcgcctt ggtgtgggcg ttgtcggtga
     1081 ggatgcggac ctgcccaccg gtgtagctct tggctttgag cccgaggtcc atcagcgcca
     1141 tggcggtcag cgcgatggtg acctgttcgc cggtagcgac catctggtcc agctcgcgtg
     1201 ggtccggggt gggcatgatc tccttggcga ggccgatcag gcggttggtc tcgccggaca
     1261 tggctgagac gacgactacg acctgatggc cgagagcctt gtaatgggcg acccggcgcg
     1321 cgacgttctg gatgcgctcg gggttggcga cggaagtgcc gccgtatttc tgtacgataa
     1381 gtgccataaa tgacctagtg ttgattcagt gtggttgatt cgatcggttt gtttttaact
     1441 cagcccagtt gctgcctgac ccagtcgttg acgctcgcca gtgcctgcgg cagtttggag
     1501 gcgtcggtgc cgcccgccat cgccatgtca ggcctgccgc cgcccttgcc gccgacttgc
     1561 gtggcgacct ggttgaccag gtcgccggcc ttgatcctgg tcgtcaggtc cttgcttacg
     1621 gcggcgacca ggctgacctt gtcgccttcg gtcacggcca gcagcagggc gcagtgaccg
     1681 agtttatcct tgagtttgtc agcgacttca cgcaggccct tggcatccac gccctcgagt
     1741 ttggcggcca gtaccttgat gccggcgata tcgaccgcct ggctggcgag atcatcgccc
     1801 tggctcgcgg ccatcttcga tttcaggcgg tcgagttcct tctccagcgc cttgacgttg
     1861 tcgatcatct ggccgatctt gcccggcagt tcctgcgcag gcgccttgaa ggcgctggcg
     1921 gcgtcgttga gcagcgcctg ttgctgctgc acatacgcta gcgccacatc gccggtggtc
     1981 gcttctacac ggcgaacacc agcggcgacg ccggattcgg catagatctt gaatagaccg
     2041 atatcgccgg tgcgctgcac atgggtgccg ccgcagagtt ctcgtgaagt accgatatcc
     2101 agtacgcgga cctcatcccc gtatttctca ccgaacagca tcatggcacc ggtcttttgt
     2161 gcggattcga tatccatcac tcgcgccagt gtcggcgcgt ttgcgatgat ctcggcattg
     2221 acgcggcgct cgacttcggt gatctcggca tcggtcatcg gcgcgttatg cacgaaatcg
     2281 aagcgggtct tttcgctgtc tacgagcgag cctttctgtt gcacatgctc gcccagcact
     2341 tcacgtagcg ccttgtgcat gagatgggtg gccgagtggt tacgcatggt gcgaccacgc
     2401 gccaccagat ccaccttggc agcgacggcg tcgccgacct tgagcgtgcc ggtcttcaat
     2461 gtgccgtggt gaccgaagac actggcctgg atcttttgcg tatcttcgac ggcgaagatg
     2521 ccatcggccg ccttgagctc gccgcgatcg ccgacctggc cgccagattc ggcgtagaac
     2581 ggcgtatcgt caagtacgac cacgcccatg tcgccttcgt cgagctgctg cacagctgcg
     2641 ccatccttgt acaacgccag gaccgtgcct ttggcttcga gtgtgtcgta accgtggaag
     2701 gtggttgcct tgccggcata ttcgagattg gccgccatct tgaacttgcc ggccgcacgg
     2761 gcttgctcct tctggcgcgc catggccgcg tcgaatgccg cggcatcgac tgtcacgccg
     2821 cgttcgcggc agatgtcggc cgtgaggtcg agcgggaaac cgtaggtatc gtgcagcttg
     2881 aatgcggtct cgccattaaa ggttgcattg ccgctgttcg ccatttcggc gagttcggtc
     2941 tcgaggatgg ccatgccgtt ctcgatggtc tcgaagaagc ggtcttcttc ctgcttcagt
     3001 acgtccatga tgcgtttctg gccgctggcg agctccgggt aagcgtctcc catctcggcg
     3061 acaaggtccg gcaccatcct gtggaagaag gcggcccgcg cgccgagctt gtatccatgg
     3121 cggatggcgc ggcggatgat gcggcgcagc acatagccgc ggccttcgtt gccggggatg
     3181 acgccgtcgg cgatgaggaa actgcaggca cggatatggt cggccagcac tttcagcgat
     3241 ggactgtcta tatcaatggg catcatttca ccattgcttc cagccatcag gccgctgcga
     3301 aatttaatcg cctcggtgct taaagcatta gtaacttcac gtgcggccgc cttgatcaag
     3361 gtctggaaca ggtcgatctc gtaattggcg tgcacgcctt gcagcacggc ggagatgcgc
     3421 tccaggccca tgccggtatc gaccgatggc ttcggcagct tgtgcatgac accagcttcg
     3481 tcacggttga actgcatgaa cacgttgttc cagatctcga tgaagcggtc gccgtcttcg
     3541 tcaggagagc cgggtggccc gccggggata tgggcgccgt ggtcatagaa gatctcgctg
     3601 catggaccgc aggggccggt gtcgcccatc atccagaagt tgtcggacgc gtagcgtgcg
     3661 cccttgttgt cgccgatgcg gacgatcttc tcggcgggga tgcctatctc cttgtgccag
     3721 atatcatagg cctcgtcgtc ctcggcatag accgtgacca gcaggcgctc ctgcggcagc
     3781 gcgaattcct tggtgatcag gttccacgcg agttcgatcg cgcgatcctt gaaatagttg
     3841 ccgaacgaaa aattgcccag catctcgaaa aacgtgtgat gacgcgcggt gtagccgacg
     3901 ttgtcgagat cgttatgctt gccgccggcg cggacgcatt tctgcgacga ggtggcgcgc
     3961 ttgtaggagc gctgctcctg gccggtgaag acattcttga actgcaccat gcccgcattg
     4021 gtgaagagca gcgtcggg
//
LOCUS       DATMTW010000115         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_9690, whole genome shotgun sequence.
ACCESSION   DATMTW010000115 DATMTW010000000
VERSION     DATMTW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9690"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1290)
                     /locus_tag="VN084_02165"
     CDS             complement(79..1290)
                     /locus_tag="VN084_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HWT28288.1"
                     /translation="MKARHSPLVISAVLASLAALAPFAIDTYLPAFPALAQALGGTTL
                     ELQQSLTAYLVPYALMTLWHGAVSDAIGRLSAIKWGLGVFALASIGCALAPNVEALWC
                     FRVLQGLSAGAGNVVARAMVRDLFEGVAAQRVMAQVQIIFGLAPAIAPIIGGFLLGIH
                     WQAIFIFLFLYAIATLFASQWVLPETVPVHKRVPLSAKQVLKGYHQAFSDTEYLRLII
                     GVGANFAGFFVYVLASPVFLIQHLGLNEQQFGAMFIPTVCGMVLGSWLARQSAGKFAP
                     HQVIRFAYTWMAAAAVLNIGISWWLPPSPWTHIAPIALYNVGMALAMPILSIAGLDRM
                     PKLRGTAASGQAFMQMLLSAVSAGMIIPLIWHHPLGLAFGMLGYLLIGLFSTRSSAVW
                     RSSQPEAGNVR"
     gene            complement(1306..1944)
                     /locus_tag="VN084_02170"
     CDS             complement(1306..1944)
                     /locus_tag="VN084_02170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWT28289.1"
                     /translation="MYEASKALMRRLHDIRFATRYFVGNGIDIGCGPDPVSQYQEQFP
                     LMRSCRSWDMPDGDAQLLASVADEAFDFVHSSHCLEHMRDPREALQHWLRVLKPGGHL
                     IVTVPDEDLYEQGIFPSTNNSDHKWTFTIAKQASWSGRSVNLCTLLSEFAVVAQVIKI
                     ELLDASFRYGLQRMDQTMTPVGECAIEFILRKWPAAELDRKGRYPDPVKKHT"
     gene            complement(1972..>2182)
                     /locus_tag="VN084_02175"
     CDS             complement(1972..>2182)
                     /locus_tag="VN084_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HWT28290.1"
                     /translation="QAFLLSGDAESADPVCSLSPNHHLIEQAIIDAQNPVLQAQPIIF
                     RTDKNSKKYASGESKERVEDFHALP"
BASE COUNT          466 a          659 c          652 g          405 t
ORIGIN      
        1 ttttttgggt cgcttttcta ttggtgggcc atttcctcgt aacgccgccc tgcgcttatt
       61 gggctcaccc tcttacattc accttacatt tcccgcctca ggctgacttg aacgccagac
      121 cgcgctgctg cgcgtactga acaagccgat cagcagatac cccagcatgc cgaaggccaa
      181 gcccagcgga tgatgccaga tgagcggaat gatcatccct gctgacaccg ccgacagcag
      241 catctgcatg aaagcctgcc cagacgcggc agtaccgcgc aacttaggca tgcggtcgag
      301 gccggcgatg gacaggatgg gcatggccaa tgccatgccg acgttataca gcgcgatggg
      361 cgcgatatgc gtccaggggc tgggtggcag ccaccagctg atgccgatgt tgagcacggc
      421 cgctgccgcc atccaagtgt aggcgaagcg gatcacctgg tgcggcgcga acttgccggc
      481 actttgccgc gccagccacg agcccagcac cataccgcag accgtgggga tgaacatggc
      541 gccgaactgc tgctcattga ggccaaggtg ctgtatgagg aacaccggcg acgccagcac
      601 atagacgaag aagccggcaa agttggcgcc gacgccgatg atgagccgca ggtattcggt
      661 atcgctaaac gcctggtgat agcccttcag tacctgtttc gccgagagcg gcacgcgttt
      721 gtgcacaggg acggtctccg gcagcaccca ttgcgaagcg aacaacgtcg ctatcgcgta
      781 caggaacagg aaaatgaaga tggcctgcca gtggatcccg agcaagaaac cgccaatgat
      841 gggcgcgatc gccggcgcca ggccaaagat gatctgcacc tgtgccatca cgcgttgcgc
      901 ggccacgccc tcgaacaggt cacgcaccat ggcccgcgcc accacgttac ccgccccggc
      961 ggacaagccc tgcaggacgc ggaaacacca cagtgcttcg acattggggg ccagcgcaca
     1021 gccgatcgac gccagcgcga acacgcccag cccccatttg atggccgaga gccggcctat
     1081 ggcatcggac accgcgccat gccagagggt catcagcgca tagggcacca ggtacgcagt
     1141 aaggctttgc tgcaactcca gcgtggtgcc gccgagcgcc tgcgccaacg ccgggaaagc
     1201 cggcaggtaa gtatcgatcg caaatggcgc caatgccgcc agactggcga gcacggccga
     1261 aatgacgagg ggagaatgtc tggctttcaa gactggctcg attccttatg tgtgtttttt
     1321 gaccgggtca gggtagcgcc cttttctgtc cagctccgcc gccggccatt tacgcagaat
     1381 gaactcgatg gcgcactcac ctaccggcgt catggtctga tccatgcgct gcaagccgta
     1441 acggaagctc gcgtcgagca gttcgatctt gatgacctgg gccaccaccg caaactctga
     1501 gagcaacgtg caaagattca ccgatcgccc tgaccagctc gcctgcttgg cgatggtgaa
     1561 ggtccatttg tggtcgctgt tattggtcga cgggaagatg ccctgctcgt agagatcctc
     1621 atccggcacg gtaacgatca ggtggccgcc cggcttcagc acgcgtagcc aatgttgcaa
     1681 ggcttcgcgc gggtcacgca tatgctccag gcaatgactg gaatgcacga aatcaaaggc
     1741 ctcgtcggca acgctcgcca gcaactgggc gtcgccgtca ggcatatccc agctgcggca
     1801 actacgcatg agggggaact gttcctggta ctgactcacc ggatcggggc cacagccgat
     1861 gtcgatgccg ttaccgacga aataacgtgt ggcaaaccga atgtcatgca ggcggcgcat
     1921 gagcgccttg ctggcttcgt acatagcgac tccgtgtgac tggagccgct attatggcag
     1981 agcgtggaaa tcctctacac gttctttgga ttcaccgctg gcatattttt tagagttctt
     2041 gtcagttctg aagatgatgg gttgcgcctg caatacagga ttctgcgcgt cgatgatcgc
     2101 ctgttcgatc aggtgatgat tgggcgagag actacagacc ggatcggcac tttcagcatc
     2161 gcctgagagc aagaaggcct gg
//
LOCUS       DATMTW010000116         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_479888, whole genome shotgun sequence.
ACCESSION   DATMTW010000116 DATMTW010000000
VERSION     DATMTW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479888"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VN084_02180"
     CDS             <1..1156
                     /locus_tag="VN084_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWT28291.1"
                     /translation="GREEARMIFVGVSHDLPPATHRRLVIDIGGGSTEFIVGEGYEPL
                     EMESLYMGCVSFSLKYFPEGKITEASLEKAHIAAASEIENIRKNFVAGHWQEAVGSSG
                     TARALGEIMRLNGLSDGEITRAGLWTLREWLLAAKDSRKLELPGLSADRAAVLPGGLT
                     IMLAAFESLGIERMTTATSALREGVLYELLGRMHHQDTREATVSHFMRRYQVDEKQSA
                     RVEQLALALLAQIEDGLRMDPETVRQYLAWAADLHEIGISIAHSGYHKHSAYIVENAD
                     MPGFSRMEQSTLGLLVRAQRRSLTKVGLPPLEDDRCAMILVLRLAILFNRNRLDEHSP
                     PLHLGWNSKGFRLEIASGWLERNPLTRAELGNEIAYWKEAGVELRVEDET"
     gene            complement(1153..>2345)
                     /gene="ppk1"
                     /locus_tag="VN084_02185"
     CDS             complement(1153..>2345)
                     /gene="ppk1"
                     /locus_tag="VN084_02185"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWT28292.1"
                     /translation="LVNLVRLMQIPDWVDRPELKFPHYTPSVPKEFAKEPDVFKAIRK
                     GDILLHHPFQSFNPVIEFIQQAAQDPDVLAIKQTFYRTSADSTLMQSLIEAAQRGKEV
                     TVVVELLARFDEEANINWAAKLEAAGAHVVYGVVGHKTHAKMAMVVRREEGKLRRYVH
                     LSTGNYHPRTARLYTDFGLLTCRDEICEDVNDVFAQLTGLGKASKLRHLWQSPFTLHQ
                     RVIKAINHEAEMARAGKKARIIAKMNALLDADIIRALYDASNAGVQIELIVRGVCALK
                     PGIPGVSEHIRVISIVGRFLEHSRIFYFYNNREENLYLSSADWMYRNFFRRIEVCFPV
                     LDVKLKKRIIKEGLEPYLRDNVNAWEMLPDSSYRLRPARRGNHFCAQTHLAVEYGQEL
                     PLES"
BASE COUNT          459 a          677 c          727 g          482 t
ORIGIN      
        1 cggccgcgaa gaggcgcgca tgatcttcgt cggtgtcagc cacgacctgc cgcccgcgac
       61 ccatagacgg ctggtcatcg atatcggcgg cggctctacc gaattcatcg tcggcgaggg
      121 ttacgaaccc ctggagatgg aaagcctgta catgggctgc gtcagcttca gtctgaagta
      181 ctttcccgaa ggcaagatca ccgaggccag cctggaaaaa gcccatatcg ctgccgccag
      241 cgagatcgag aacatccgca agaattttgt cgccggtcac tggcaggaag ctgtaggttc
      301 gtccggtaca gcacgcgcgc tgggggagat catgcggctc aatggcctta gcgacggcga
      361 gatcacccgc gccggcttgt ggaccctgcg tgaatggttg ctggccgcca aggacagccg
      421 caagctcgaa ttgcccggcc ttagcgccga tcgcgcagcg gtgctgccag gtggcctcac
      481 catcatgctt gcggcgttcg agtcgctcgg tatcgaacgc atgaccacag ctaccagtgc
      541 cttgcgtgaa ggcgtgctct acgagctgct gggccgcatg catcatcagg atacgcgtga
      601 ggcgactgtc tcgcatttca tgcggcgcta tcaggtcgac gagaagcaat ccgcgcgggt
      661 cgaacagctt gcgctggcat tgctggcaca gatcgaagac ggtttgcgca tggatccgga
      721 aaccgtgcgt cagtatctgg cctgggcggc ggatttgcac gagatcggca tctccatcgc
      781 gcacagtggc tatcacaagc attccgccta tatcgtcgag aatgccgata tgccggggtt
      841 ctcgcgcatg gagcaatcca cgctcggcct gctggtgcgg gctcagcgcc gttcactgac
      901 caaagtcggc ttgccgccgc ttgaagacga tcgttgtgca atgatcctgg tgctgcgact
      961 ggcgatcctg ttcaatcgca atcggctcga tgaacatagc ccgcctttgc acctgggttg
     1021 gaacagcaag ggattccgcc tcgagatcgc gtcgggctgg ctggagcgca accctctaac
     1081 ccgtgccgaa ttgggtaacg agatcgcgta ctggaaagag gctggcgtcg agttgcgcgt
     1141 cgaggacgag acctagctct cgagcggcag ctcctgcccg tattccacag cgaggtgggt
     1201 ctgtgcgcag aaatggttac cgcgccgggc cggtctcaaa cgatagctgc tgtccggcag
     1261 catctcccaa gcattgacgt tgtcccgcaa gtaaggctcc aggccttcct tgatgatgcg
     1321 ctttttgagc ttgacgtcca gcaccggaaa acacacttcg atgcgccgga agaagttgcg
     1381 gtacatccag tctgcgctcg acagatacag gttctcttca cggttgttat agaaatagaa
     1441 gatacgcgag tgttcgagga atcgcccgac gatggagatg acgcggatat gttcggatac
     1501 gcccgggata cccggcttga gcgcacaaac gccgcgcacg atgagttcga tctgcacgcc
     1561 ggcattggag gcatcgtaga gtgcgcggat gatatcggcg tcgagcaggg cgttcatctt
     1621 ggcgatgatg cgcgccttct tgcctgcgcg tgccatctcg gcttcatggt tgatggcctt
     1681 gatcacgcgt tgatgcaagg taaagggtga ctgccagagg tggcgcaact tgctcgcttt
     1741 gccgagacct gtcagctggg cgaagacatc attgacgtcc tcgcagatct cgtcgcggca
     1801 ggtgagtagg ccgaaatcgg tatagaggcg ggccgtgcgt ggatggtagt tgccagtgga
     1861 gagatgtacg taacggcgta gcttgccttc ctcgcgccgc accaccatcg ccatcttggc
     1921 gtgcgtctta tggcccacca cgccatacac cacatgcgcg ccagccgctt ccagcttggc
     1981 tgcccagttg atgttggctt cctcgtcgaa acgcgccagt agctcgacca ccacggtcac
     2041 ttccttgcca cgctgtgcgg cctcgatcag cgactgcatg agcgttgagt cggcactggt
     2101 acgatagaag gtctgtttga tcgccagcac gtcagggtcc tgcgccgcct gctggatgaa
     2161 ctcaatgacc ggattgaacg actggaaagg gtgatgcagc aggatatcgc ctttgcggat
     2221 ggccttgaac acatccggct ccttggcgaa ttctttcggg acgctggggg tgtaatgcgg
     2281 gaacttgagt tcaggccgat ccacccaatc cggaatctgc atcaggcgca cgaggttcac
     2341 caggc
//
LOCUS       DATMTW010000117         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_412271, whole genome shotgun sequence.
ACCESSION   DATMTW010000117 DATMTW010000000
VERSION     DATMTW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412271"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VN084_02190"
     CDS             <1..739
                     /locus_tag="VN084_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWT28293.1"
                     /translation="RAASMLNAIVAISMLTTPLLMLIYGRWAQRSLNRPAEGHDDFET
                     QRPIIIAGFGRVGQVLTRLLNGIGTPPTVIELDPNQIELVRQFGFKAYYGDITRPDVL
                     EAAGIAQARLFVLAIDDPEDALETARYVKAHYPQVRILARARNRTHAFDFMELEIDVV
                     RETFFSALQLAERMLREIGYTQAQANRAVQRFRKHDEHMMQESLAIRHDDEKLIENSH
                     RAREDLARLLQQEFEEAVTEVPAAEAR"
     gene            complement(736..1164)
                     /locus_tag="VN084_02195"
     CDS             complement(736..1164)
                     /locus_tag="VN084_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWT28294.1"
                     /translation="MKTVSQLLETKGHQIYSVAPDSLVYDALLLMAEHHIGALLVMRR
                     DELVGIFSERDYAREVVIKGRTSATTAVQDIMSTKLITVSPEDTVEACMNLMSGKRIR
                     HLPVLDHGKVVGLLSIGDLVQATIEYQQFLIKQLESYIQG"
     gene            1281..1790
                     /locus_tag="VN084_02200"
     CDS             1281..1790
                     /locus_tag="VN084_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWT28295.1"
                     /translation="MLQLRDLPTPEVLNKFAQRYPQADISAISSFLHLLRVATDLSIA
                     LDECLSRHGLLQGRWWVLILLMREEALVSTPSALADKAGVTRATMTGLIDGLERDGLV
                     ERIFEPQDRRRISVKLTRAGQDRLDAVMPDYYSRLRECMSALDEAQRAGLHQMLDLIS
                     TGVYAFTKA"
     gene            1873..2268
                     /locus_tag="VN084_02205"
     CDS             1873..2268
                     /locus_tag="VN084_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HWT28296.1"
                     /translation="MRDMTETPVDTPALLQTLNSRWDEAFNSQQPTVLAALYDDEATV
                     MPAGAAPASGGRAILEFWRNLIAQGVVDHKIEIVETIVDGNLGVQRGFWSAAAVDADG
                     KRQVFRGSLQLVFRRQIDGSWKTYTHIWN"
     gene            2298..3212
                     /locus_tag="VN084_02210"
     CDS             2298..3212
                     /locus_tag="VN084_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWT28297.1"
                     /translation="MSNTSQTVRAFLALLLLAALWGYNWVVMKHALDFAGAFQFGALR
                     TFLGAVCLMLLLLVMRRPLKPAEVPTLILLGILQTCGFTGLIIWALVEGGAGKTAVLC
                     YTMPFWTMLLAWPLLGEKIKGWNWLAVGLSVMGLLFILDPLHLGADWFSMILAILGGV
                     FWALAVIVAKKLHQRKPDLDLLSLTAWQMLFGSLPLVAVAFIVPAGPIDWTPQFIGAV
                     IFNAVFCNALAWLLWLYALQRLAAGVASMTSMLAPVIGVLAAWWQLDEVPNTYEGVGM
                     VLIGLALVVISIISIRKHTEVEPAMGQD"
     gene            3485..>4038
                     /locus_tag="VN084_02215"
     CDS             3485..>4038
                     /locus_tag="VN084_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWT28298.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKIFTKLIKEITVAARLGGGDIN
                     FNPRLRAAVAEAKEENMPADNITRAIKRGTGELEGVSYEEIRYEGYGINGAAIIIDCL
                     TDNKQRAVADVRHALSKNGGNLGTDGSVAFLFKHCGSLLFAQGTSEDQVMEAALEAGA
                     EDIITDEDGSIEVITAPSDFSNVK"
BASE COUNT          793 a         1194 c         1208 g          843 t
ORIGIN      
        1 cagggcggca agcatgctca acgccatcgt tgccatctcc atgctgacca cacctctact
       61 catgctgatc tatggccgct gggcacagcg cagcctcaac cgtccggcag aggggcatga
      121 cgacttcgag acccagcgcc ccatcatcat cgctggtttc ggccgtgtgg ggcaggtgtt
      181 gacgcgtctc ttgaacggca tcggcacgcc gccgacagtg atcgagctcg accccaatca
      241 gatcgagctg gtgcggcagt tcggcttcaa ggcctattac ggcgacatca cccgaccgga
      301 cgtgctggaa gcagcgggta tcgcccaggc acgcctgttc gtgctggcga tcgacgatcc
      361 ggaagatgcg ctggagacgg cgcgctatgt gaaggcgcat tatccgcagg tgcgcatcct
      421 ggcaagggcg cgcaaccgga cgcatgcttt cgatttcatg gagctggaaa tcgacgtcgt
      481 gcgcgagacc ttcttctcgg ccctgcaact ggcggaaagg atgctgcgcg agatcggcta
      541 tacccaggcg caggcgaacc gtgccgtaca gcgattccgc aagcacgacg agcacatgat
      601 gcaggagtcg ctggcgatcc ggcatgacga cgagaaactg atcgagaaca gccaccgtgc
      661 acgcgaggat ctggcccggt tgctgcaaca ggaattcgag gaggccgtga ccgaggtgcc
      721 ggccgcggaa gcacgctagc cctggatgta gctttccagt tgcttgatca ggaattgctg
      781 gtattcgatg gtcgcctgca ccaggtcgcc gatcgacagc agcccgacca ccttgccgtg
      841 atcgagcacc ggcagatggc ggatacgttt gcctgacatc aggttcatgc acgcctcgac
      901 ggtgtcttct ggcgataccg tgatgagttt ggtcgacatg atgtcctgga cggcggtggt
      961 cgcggaggtg cggcccttga tgacgacttc gcgggcatag tcccgttccg agaagatccc
     1021 caccagttcg tcgcggcgca tgacgagcag ggcgccgatg tggtgttcgg ccattaacag
     1081 cagcgcatcg tagaccaggc tatccggcgc cactgagtag atctgatgac ctttcgtctc
     1141 aagcaattgg ctgacggtct tcatcctatc ctcctcattt cttgtcttgt tcttgatgac
     1201 taaaaatata caccttaatg ccctgagcca aaacattcgc gcttgacata gttagccaac
     1261 taactataat gacactaacc atgttgcaat tacgcgatct acccacaccg gaagtcctga
     1321 ataaatttgc ccagcgttat ccgcaggcag atatcagtgc catttcctct tttctgcatc
     1381 tgctgcgcgt ggccaccgac ctttccatcg cgcttgacga gtgcctgagc cgccatggct
     1441 tgctgcaggg acgctggtgg gtgctgatcc tgctgatgcg tgaggaggcg ctggtctcaa
     1501 cgccgtcggc gctggccgac aaggcgggtg tgacgcgcgc caccatgacc ggcctgatcg
     1561 acggtctgga gcgtgatggt ctggtcgagc gcatctttga accgcaagac cgccgccgga
     1621 tctcggtcaa gctcacccgg gctgggcagg atcggttgga tgccgtcatg cccgattact
     1681 atagccggct gcgcgaatgc atgtcggcgc tggatgaagc gcagcgtgca gggctgcatc
     1741 agatgctgga tctgatctcg accggcgtgt acgcgttcac caaagcctga accttactta
     1801 aagccgtttt ttttagccaa acaattaggc gactaatcat ttattttatt gacccattca
     1861 aaggagtcca tgatgcgaga tatgactgaa accccggtgg atacccccgc cttactgcaa
     1921 acgctcaaca gccgttggga cgaagccttc aatagccagc aaccgaccgt gctggcggcg
     1981 ctgtacgacg atgaggcgac cgtgatgcca gcgggtgctg cgcccgcgag tggtggtcgg
     2041 gcgatcctcg agttctggcg taacctcatt gcgcagggtg tcgtcgacca taagatcgag
     2101 atcgtcgaaa ccatcgtcga cggcaacctg ggcgtgcagc gtggattctg gagtgctgcc
     2161 gcggtcgatg ctgatggcaa gcgccaggtc ttccgaggta gtctgcaact ggtgttccgt
     2221 cggcagatcg acggcagctg gaagacctat acacacatct ggaactaagc gacacttgcc
     2281 aaaaacggga agccaccatg tccaatactt cacaaaccgt acgcgccttc ctcgccttgc
     2341 tgctgcttgc ggcgctctgg ggctataact gggtcgtcat gaagcatgcg ctggattttg
     2401 ccggcgcctt ccagttcggt gcgctgcgga cctttcttgg cgcggtctgc ctcatgctgc
     2461 tgcttctggt catgcgccgg ccgctcaagc cggcggaagt gccgaccctg atcctgctcg
     2521 gcatcctgca gacctgcggc ttcaccgggc tcatcatctg ggccctggtc gaaggcggtg
     2581 caggaaagac cgccgtactt tgctacacca tgccgttctg gaccatgctg ctggcctggc
     2641 cattgttggg cgagaagatt aagggctgga actggctggc ggtgggactc tcggtcatgg
     2701 gtctgctatt catcctcgac ccgctgcatc tgggggctga ctggttcagc atgatcctcg
     2761 ccatcctcgg tggggtgttc tgggcactgg ccgtcatcgt cgccaagaag ctgcaccagc
     2821 gtaaaccgga tctcgatcta ctttcgctga cggcatggca gatgctgttc ggttccctgc
     2881 cgctggtggc cgtggctttc atcgtgccgg ccggtccgat cgactggacg ccgcagttca
     2941 tcggcgccgt catctttaat gccgtgttct gtaacgcgct ggcctggctg ctatggctct
     3001 atgccctgca gcgccttgcc gcaggcgtcg ccagcatgac ttcgatgctg gccccggtga
     3061 tcggcgttct tgccgcctgg tggcaactcg acgaagtgcc taacacttat gagggcgtcg
     3121 gcatggtgct gatcggtctg gcgctggtgg tgatctccat catcagtatc aggaagcata
     3181 cggaagtcga gcctgccatg ggccaggact gacacgtcgc ctgatcaggc gaactttgtt
     3241 gcgccggctt gccgtaccaa ggtgtactgt cttcgccgac gcgcctcgtt cgcccctctc
     3301 agcctggggt gtctcgatcc tccatgtaat acgaagtgaa cgctggtcaa gcaatcctgc
     3361 cgatagcgat cgaggccggc catgccccag ctagagactg ctttgcaaag cgaaagctca
     3421 cgttgggccg gcttggtgcc tatgctaaaa tcccagcagt tttcagacac caagggtttc
     3481 agacatggca gggcattcca aatgggccaa tatccagcat cgcaagggcc gtcaggacgc
     3541 taagcgcggc aagatattca ccaaactcat caaggaaatc acggttgccg cgcggctcgg
     3601 cggcggtgac atcaacttca atcctcgctt gcgcgcagcg gtggctgaag ccaaggaaga
     3661 gaacatgccg gccgacaaca tcacgcgtgc catcaagcgc gggaccggcg agctcgaagg
     3721 cgtcagctac gaagagatcc gttatgaagg ctacggtatc aacggcgccg ccatcatcat
     3781 cgattgcctg actgacaaca agcagcgtgc tgtcgccgat gtgcgtcacg ccctgagcaa
     3841 gaacggcggc aatctcggga ctgacggatc ggtcgctttc ctgttcaagc attgcggcag
     3901 cctgctgttt gcgcaaggca ccagcgagga tcaggtcatg gaagccgcgc tcgaagcagg
     3961 cgccgaggac atcatcaccg atgaggacgg cagcatcgaa gtcatcaccg cgccatccga
     4021 tttcagtaac gtgaagga
//
LOCUS       DATMTW010000118         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_546755, whole genome shotgun sequence.
ACCESSION   DATMTW010000118 DATMTW010000000
VERSION     DATMTW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546755"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..611
                     /locus_tag="VN084_02220"
     CDS             276..611
                     /locus_tag="VN084_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28299.1"
                     /translation="MTGFIPLSSLWRFTPTDQARSKEGAAMATIDTENLAKGLQQVEA
                     CWQAYTSSQRLVEGLKGSDLSSAIETANRDFTRFQNQCQLFYAEVRKRAADADKLESD
                     MARYPHYFQ"
     gene            678..920
                     /locus_tag="VN084_02225"
     CDS             678..920
                     /locus_tag="VN084_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28300.1"
                     /translation="MSIERNIPELEAWYAQYDDISYRRASPDAYHHELLRTAEALRDD
                     GAIDWDDWLALKALADEAHIRAMEDAVQAHVSDPDA"
     gene            917..>1633
                     /gene="xth"
                     /locus_tag="VN084_02230"
     CDS             917..>1633
                     /gene="xth"
                     /locus_tag="VN084_02230"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWT28301.1"
                     /translation="MKALKIATFNINGIRSRLPALLAWLEREQPDIACLQELKAADQQ
                     FPRAELEAAGYGSLHHGQPSWNGVAILVRGSDPLEVRRGLPGLEEDSQSRYLEAAAHG
                     VLVACLYLPNGNPQPGPKFDYKLHWFECLIQHARSLYGSGHPTLLAGDFNVVPTDMDI
                     YNPRSWLKDALLQPESRACYQRLLDQGWLDAIRHLYPDERVYTFWDYFRNHWARNAGL
                     RIDHLLLNRELAPYLKRAGVD"
BASE COUNT          341 a          483 c          495 g          314 t
ORIGIN      
        1 atatcacgca catagatcaa tgcgccgtct tccgggtaag gccacggcac acgacggtcc
       61 agataacgga cgatctccca ctgggggaac agtttctgaa tggttgctgc atctcccaac
      121 tgaaggggtg tgagaacaag acggtctgtg aacagcgttg gaaggcaatc catggcactc
      181 gctcctgagc ttgcggataa tgcgtctgag acttgtgaaa cctggtatcc atgcgtctga
      241 gccgaaatag tctacgctct tagccgaagc gcgtcgtgac aggcttcatc ccgttatcgt
      301 cgctctggcg attcacaccg acggaccagg ctcggtccaa agagggtgct gcaatggcga
      361 ctatcgacac cgagaacctt gcaaaggggc tgcagcaggt tgaagcctgc tggcaggcgt
      421 atacctcgtc acagcggctt gtcgaaggcc tcaagggcag cgacctgagc agtgccattg
      481 aaaccgccaa ccgcgatttc accaggtttc agaaccagtg ccagcttttt tatgccgaag
      541 tgcgcaagag ggccgcggat gcggacaagc tggagagcga catggcacgg tatccccatt
      601 actttcagtg acgtcctgat tgaacgtcgc gctgtgcccg ggttctgaca agcaaagggt
      661 acagcgagga cgctttcatg agcatcgaac gcaacattcc cgagcttgaa gcctggtacg
      721 cccagtacga cgacatcagt taccggcgcg cgtcaccgga tgcctaccac catgaactgc
      781 ttcgcacagc cgaggccctg cgcgacgatg gcgccatcga ctgggacgac tggttggcgc
      841 tcaaggcgct ggccgatgag gcgcatatcc gcgcgatgga agatgccgtg caggcccatg
      901 tgagtgaccc ggacgcatga aagcgctgaa gattgccacc ttcaacatca atggcatccg
      961 cagccgtttg ccggcgctgc tggcctggct ggagcgggag cagcccgaca ttgcctgcct
     1021 gcaggagctg aaggcggccg atcagcagtt ccctcgcgcg gagctggagg cagcaggcta
     1081 tggcagcctt catcatgggc aaccgtcatg gaacggcgtg gccattctgg tgcggggcag
     1141 tgatccgctt gaggttcgcc gcggcttgcc cggcctggaa gaggacagcc agagccgcta
     1201 tctggaagcg gcagcacacg gggtgttggt cgcttgcctc tacctgccga acggcaaccc
     1261 gcagcccggg cccaagttcg attacaagct gcactggttc gaatgcctga tccagcatgc
     1321 gcgcagcctc tatggaagtg gccaccccac gctgctggca ggggatttca atgtcgtgcc
     1381 caccgacatg gacatctata acccgcgttc ctggctgaag gacgcactgc tgcagcctga
     1441 gtcgcgagcg tgttaccaac gactgcttga ccagggctgg ctcgatgcca tacgccatct
     1501 gtacccggac gagcgtgtct acaccttctg ggactatttc cgtaaccact gggcccgcaa
     1561 tgccgggttg cgaatcgatc acctgttgct caatcgcgag cttgcgcctt atctgaaacg
     1621 ggcgggtgtc gat
//
LOCUS       DATMTW010000119         4508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_546766, whole genome shotgun sequence.
ACCESSION   DATMTW010000119 DATMTW010000000
VERSION     DATMTW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546766"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1792)
                     /locus_tag="VN084_02235"
     CDS             complement(119..1792)
                     /locus_tag="VN084_02235"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWT28302.1"
                     /translation="MYKYDEIDQAIVDERVAQYRDQVRRFLAGELSDDEFRPLRLQNG
                     LYIQRHAPMLRIAVPYGTLASRQLRKLGDIAKRYDRGYGHFSTRQNMQLNWPKLEDTP
                     DILAELATVQMHAIQTSGNCIRNITTDQFAGIAPDEIIDPRPIAEIMRQWSTFHPEFA
                     LLPRKFKIAVSGSAQDRAIVQAHDIGVEFYYDDSKKIAAKVWVGGGLGRTPILGEVIR
                     EHIEWQHVLTYCEAIIRVYNLHGRRDNAFKARIKILVKALGIDEFRRQVEAEWLHLKD
                     SPNTITEAEFARVSQFFTTMPYETLPAHDSGFDAAIASNPAFAAWVKRCVHGHKMPGY
                     RALTLSLKPHGKAPGDATSEQMYVVADLADTYSFGELRVSHEQNLILTDVKLSDVYAV
                     WEIARANGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQMQFDNLDYLYDIGE
                     LELNISGCMNACGHHHIGHIGILGVDKDGSEWYQVTIGGKQGHDASLGTVIGPSFSAD
                     EMPGVVQRLIEVYLAQRTPEERFIDTARRIGVAPFKEHVYAGRKEAAHV"
     gene            complement(1836..2630)
                     /locus_tag="VN084_02240"
     CDS             complement(1836..2630)
                     /locus_tag="VN084_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWT28303.1"
                     /translation="MDLGYIVSGFAVGMLVGITGVGGGSLMTPLLVFLFGFKPAVAVG
                     TDLLFAAITKTGGVWVHHGKHGSVDWRVVSLMSVGSLPMAIGTIFVIRQLIAVGKETS
                     GIITYSLGVALMLTACALLVRSYVTRNTQRETENGLVSTGRFKRMQVPATILTGAVLG
                     VLVTLSSVGAGALGTIAILFLYPKMRTLKVVGTDLAHAIPLTAVAGIGHWSLGHVDFL
                     LLGSLLVGSLPGIWIGSHISAKIPEKVLRPILASILLLIGLKFVVN"
     gene            2755..3681
                     /locus_tag="VN084_02245"
     CDS             2755..3681
                     /locus_tag="VN084_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HWT28304.1"
                     /translation="MKLHQLRYVREVARHGMNVSLAAEALHTSQPGVSKQIQLLEEEL
                     SLQIFQRNGKRLIGVTEPGRIILELAERVMREMDNIKRVGEEFTHEETGHLTVATTHT
                     QARYRLPAAVKAFMERFPSVKLTIHQGNPTQVSEQVANGEADIGIATESISSYQNLLC
                     LPCYQWNRCVVMPHGHPLLNDQPLTLEKITRYPLITYDFAFTGGSLVSKVFGKEGLTP
                     NVVLTAIDADVIKTYVSLGLGVGLLAKMAYDAERDANLAMLDVSHLFPPSTTYLGVRK
                     DAYLRGYTYEFIQLLAPQFDRKAVKSALQTDV"
     gene            3860..4360
                     /locus_tag="VN084_02250"
     CDS             3860..4360
                     /locus_tag="VN084_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWT28305.1"
                     /translation="MRDSLVFAALSLLLSTPVAWAAAPLPPVDESTAIYEVPVEPGVS
                     YDDVVTSLKVVSEGENFVNPANFPIGEHMKQRGQDPHGPLEVRAFCNLGLGAEIMLDH
                     PEFVVFAPCRIAIYQKQGQLYLGLDRPTYDLKSIKHPTARAVKAAQQLEATLIAIIDK
                     ARKGDI"
     gene            4396..>4508
                     /locus_tag="VN084_02255"
     CDS             4396..>4508
                     /locus_tag="VN084_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HWT28306.1"
                     /translation="MAEPILIAKAGDRDLCILPRMANRHGLIAGATGTGKTV"
BASE COUNT          915 a         1307 c         1366 g          920 t
ORIGIN      
        1 gagcttgaac atcacgactt taccagcctg cttgcggacg atcttctcgc cgatctccgg
       61 cagttggacg gtctgccatt cgtcggcgac gatctggcca ttttgtatga gattggattt
      121 agacatgagc tgcctccttc ctgccggcat agacatgttc cttgaatggc gcgacaccga
      181 tacgacgcgc ggtatcgata aagcgttctt ctggcgtgcg ttgcgccagg tagacctcga
      241 tcaggcgttg cacgacgccc ggcatctcgt ctgccgagaa cgatgggccg atgacggtgc
      301 cgagactggc gtcatggcct tgcttgccgc cgatggtgac ctggtaccac tcggaaccgt
      361 ccttatcgac gccaaggatg ccgatatggc cgatgtggtg gtgaccacag gcattcatgc
      421 agccagagat gttgagctcg agctccccaa tatcgtaaag gtagtcgagg ttgtcgaact
      481 gcatctggat ggcttcggcg atggggatgc tcttggcgtt cgccaggctg cagaagtcgc
      541 cgccggggca gcagatgatg tcggtcagca agccgatgtt gggggtggcg aggccattgg
      601 cgcgtgcgat ctcccagacg gcatagacgt cgctgagctt gacgtcggtc aggatcaggt
      661 tctgctcgtg cgatacgcgc agctcgccga aactgtatgt atccgcaaga tcggcgacca
      721 catacatctg ttctgaagtg gcgtcgcccg gggccttgcc gtgaggcttt agcgacaggg
      781 tcaaggcgcg atagccaggc atcttgtggc catgcacgca gcgtttgacc caggcggcga
      841 acgccgggtt gctggcgatg gcggcgtcga agccgctgtc atgcgcaggc agcgtctcgt
      901 aaggcatggt cgtgaagaac tggctgacgc gcgcgaactc ggcttcggtg atggtgttgg
      961 ggctatcctt caggtgcagc cattcggcct cgacctggcg gcggaactcg tcgattccga
     1021 gtgccttgac caggatcttg atgcgcgcct tgaacgcgtt gtcgcggcgg ccatgcaggt
     1081 tgtagacgcg gatgatggcc tcgcaatagg tcagcacgtg ctgccattcg atatgttcgc
     1141 ggatgacttc acccaggatc ggcgtgcggc ctaggccacc gccaacccat actttggctg
     1201 cgatcttctt gctgtcgtcg tagtagaact cgacgccgat gtcgtgcgcc tgcacgatgg
     1261 cgcggtcctg cgcgctgccg gagacggcga tcttgaactt gcgcggcaag agcgcgaatt
     1321 ccgggtggaa ggtactccac tggcgcatga tctcggcgat cgggcgtgga tcgatgatct
     1381 cgtccggggc gatgccggcg aactggtcgg tggtgatgtt gcggatgcag ttgcctgagg
     1441 tctggatcgc gtgcatctgc acggttgcca gctcggccag gatgtccggt gtgtcttcca
     1501 gcttgggcca gttgagctgc atgttctgac gggtgctgaa gtgaccatag ccacggtcgt
     1561 aacgcttggc gatgtcgcca agcttgcgca gctgacgcga ggccagtgtg ccgtaaggca
     1621 cggcgatacg cagcatcggc gcatggcgct ggatgtagag gccgttctgc aggcgcagcg
     1681 ggcggaattc gtcatcgctc agttcgcctg ccaggaagcg gcgtacctgg tcgcgatact
     1741 gcgcgacgcg ctcgtctacg atggcctgat caatctcgtc gtatttatac atagtctctt
     1801 acgtctattc gtccgtgttg gttcaatgct acaagtcagt tcactacaaa tttcagcccg
     1861 atcagcaaca ggatcgaagc gaggatcggg cgcaggacct tttccgggat cttggcgctg
     1921 atgtggctgc cgatccagat gcctggcagc gaacccacca gcaggctgcc cagcagcaag
     1981 aagtccacat gtcccagcga ccagtggccg atgccggcga ccgcagtcaa cgggatggca
     2041 tgcgccagat cggtgccaac cactttcagc gtccgcatct tcggatacag gaacaggatg
     2101 gcgatcgtgc ccaatgcacc tgcgccgacc gaggacagcg tgacaagcac gccgagcacc
     2161 gccccggtaa ggatggtcgc cggcacttgc atgcgcttga aacggccggt actgaccagg
     2221 ccgttctcgg tctcgcgttg cgtgttgcgc gtgacgtagc tgcggaccag cagggcacag
     2281 gcggtcagca tcagcgccac gcccagcgaa tacgtgatga tgccgctcgt ttccttgccg
     2341 acggcgatca gttgccgaat gacgaagatg gtgccgatcg ccatcggcag gctaccgacc
     2401 gacatcaggc tcacgacacg ccaatcgacc gagccgtgct tgccatggtg tacccagacg
     2461 ccgccagtct tggtgatcgc tgcgaacagc agatcggtac cgaccgctac ggctggcttg
     2521 aatccgaaca ggaacaccag cagcggcgtc atcagggagc cgccgcccac ccccgtgatc
     2581 cccaccagca tgccgactgc gaagccggaa acgatataac ctaagtccat gaaatttctt
     2641 tgaatattcg gctcaaggca cgaagaggca gttattttaa cagctaagta tctggtgttt
     2701 aataatatgt tattctatga atatacattt tcgttataaa gcgggagcct gaccatgaag
     2761 ctgcatcagt tgcgctatgt gcgcgaagtg gcacgtcacg gcatgaacgt atcactagcg
     2821 gcggaagcgt tgcatacgtc gcagccgggc gtcagtaagc agatccaact actggaggaa
     2881 gagctcagcc tgcagatctt ccagcgcaac ggcaaacgct tgatcggtgt cacggaaccg
     2941 ggccgaatta tactggagtt ggctgagcgc gtcatgcgcg agatggataa catcaagcgt
     3001 gtcggcgagg agttcacgca tgaagagact ggccacctga cggtggcgac gacccacacg
     3061 caagcgcgct atcgcctgcc ggccgcggtc aaggccttca tggaacggtt cccctcggtc
     3121 aagctgacca ttcaccaggg caacccgacc caagtctcgg aacaggtcgc caacggcgaa
     3181 gccgatatcg gtatcgctac cgagtccatc agcagttacc agaatctcct ttgcttacct
     3241 tgttatcagt ggaatcgttg cgtggtcatg ccgcacggac atcccttgct caacgatcaa
     3301 ccgttaaccc tggaaaagat cacgcgttat ccactcatca cctacgattt cgcctttaca
     3361 ggcggttcgc tggtaagcaa ggtgttcggc aaggaagggc tgacgcccaa cgtggtgctg
     3421 acggcgatcg atgccgacgt catcaagacc tacgtctcgc ttgggctggg cgtgggttta
     3481 ttggcaaaga tggcctatga cgccgagcgc gacgccaatc tggcgatgct tgacgtgagc
     3541 cacttgttcc cgccgagcac cacttatctt ggggtgcgca aggacgctta tctgcggggt
     3601 tacacgtacg agttcatcca gctcttggcg ccgcagttcg atcgtaaggc cgtcaaatcg
     3661 gcgttgcaga ccgacgtctg agtctgcgcc atctttattg agtctgcgcc atcgtgacga
     3721 tgcatctttc tcgaacgcat gacggtgcca cctgtcgatc ggctcacgaa tgccgccgat
     3781 cggctggcag ggcggaaagc ggggtatgat gaccacagtc gtgatattgg ttaagagtta
     3841 caggaaaggt cgatcgctga tgcgtgattc actggtattt gcagcgttgt cactgctttt
     3901 gagtacgcct gtcgcatggg ccgccgcacc gctgccgccg gtcgacgaaa gcaccgcgat
     3961 ctacgaggtg ccggtcgaac ccggcgtcag ctacgacgat gtggtgacca gcctcaaagt
     4021 cgtctccgaa ggcgagaact tcgtcaaccc ggccaatttc cccatcggcg aacacatgaa
     4081 gcagcgcggc caggatccgc atggcccgct cgaagtgcgc gccttctgta accttgggtt
     4141 gggcgcagag atcatgctcg accatccgga attcgtcgtc ttcgctcctt gccgcatcgc
     4201 catctatcag aagcagggcc aactctatct cgggctggac cgaccgacct acgatctcaa
     4261 gagcatcaag catccgaccg cgcgcgccgt gaaagccgcg caacagctcg aggcgacctt
     4321 gatcgcgatc atcgacaagg cgcgcaaggg cgatatctga acgaatcaac aacccataaa
     4381 aacaaaaggt gaaacatggc tgaaccgatc ctgatcgcca aggctggcga tagagacctt
     4441 tgcatcctcc ctcgcatggc caaccgacac ggcctgatcg caggcgctac cggcaccggc
     4501 aagaccgt
//
LOCUS       DATMTW010000120         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_111090, whole genome shotgun sequence.
ACCESSION   DATMTW010000120 DATMTW010000000
VERSION     DATMTW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111090"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /gene="lpdA"
                     /locus_tag="VN084_02260"
     CDS             complement(<1..1567)
                     /gene="lpdA"
                     /locus_tag="VN084_02260"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWT28307.1"
                     /translation="MSQLTEVLVPDIGNFDSVDVIEVLVKAGDVIAKEDSLITLESDK
                     ASMDIPSSHAGTVKEVKVKVGDKVAKGTLILLVEAEAATEAKPAPAAAPAAAPVPVAE
                     TKPATVTAPTPAPAAVGASDLHAEVVVLGSGPGGYTAAFRAADLGKKVVLIERYATLG
                     GVCLNVGCIPSKALLHTAKVITEAEETAHHGVSFGKPQIKLEELRNWKANDVVAKLTG
                     GLAQMAKQREVTTVVGVGKFTGPNQIAVTAADGKVTTVSFDNAIIAAGSQATKFPGAP
                     EDERIMDSTGALALADIPKRLLVIGGGIIGLEMGTVYDALGSKVSVVEFMDGLIPGCD
                     RDLVRPLQKRMEKRFEKIMLSTKVSKIEAKKDGIHVSFEGENAIEPQVYDRVLVAIGR
                     RPNGKNIGAELAGVAVDERGFIAVDKQMRTNVPHIFAIGDIVGQPMLAHKATHEGKVA
                     AEVIAGHKVEFQALAIPSVAYTDPEVAWAGVSETEAKAKGIEIEKASFPWAASGRALS
                     IARSEGSTKLIFDK"
     gene            complement(1577..2905)
                     /gene="aceF"
                     /locus_tag="VN084_02265"
     CDS             complement(1577..2905)
                     /gene="aceF"
                     /locus_tag="VN084_02265"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HWT28308.1"
                     /translation="MAIKDVLVPDIGNFDSVDVIEVLVKAGDSIAKDDSLITLESDKA
                     SMDIPAPFGGTVKEVKLKVGDKVAQGTLILTVDAAEEQATAAKPAPAPTAPPVVTAVQ
                     EHAIPAPSHPVPEAPKPIVPAHKPDPIGASVVTNGGKRSHASPSVRKFARELGVNLAL
                     VQGSGPKDRILQSDVQAFVKSELSKPRSEMAAGAGLSTLPMPVIDFSQFGEIESKPLS
                     RIKKLSGANLHRNWVTAPHVTQFDEADITDLEDFRKSMLAEAEKRGVKLTLLAFLMKA
                     VVNALRTYPNFNASLSPDGESLIMKQYFHVGFACDTPDGLVVPVVRDVNQKDVLDIAR
                     DLAELSAKARERKLKIEEMQGGCFTISSLGGIGGTMFTPIINCPEVAILGVSRSSFQP
                     VFDPKSKSFEPRLILPLSLSYDHRVIDGADGARFTSHLRMMLSDVRRLLL"
     gene            complement(2923..>5145)
                     /gene="aceE"
                     /locus_tag="VN084_02270"
     CDS             complement(2923..>5145)
                     /gene="aceE"
                     /locus_tag="VN084_02270"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWT28309.1"
                     /translation="VYARAFLEGRLTEEQLSNFRIETEGKGLSSYPHPWLMPDFWQFP
                     TVSMGLGPLQGIYQARFLKYLHDRGIADTSDRKVWVFCGDGEMDEPESLGAISLAARE
                     KLDNLIFVINCNLQRLDGPVRGNGKIIQELEADFRGSGWNVLKVIWGSYWDPLLSMDK
                     DGLLKKRMEEAVDGEYQNFKAKGGAYTREHFFGKYPELKDMVAAMSDEDIWRLNRGGH
                     DPHKVYAAYAAATSHKGQPTVILAKTVKGYGMGEAGEGHNTTHQQKSMDIASLKAFRD
                     RFDLPLTDEQVENLSFYKPADDSPEMQYMRERRNALGGYVPSRRRKGNELTVPELTAF
                     ENMLGATGEREISTTMAFVRILSTLVRDKQLGKYVVPIVPDEARTFGMEGMFRQLGIY
                     SSVGQLYEPQDSDQVMFYKEQKDGQILEEGINEAGSFSSWIAAATSYSVTGTQMIPFY
                     IFYSMFGFQRIGDLAWAAGDSRARGFLLGATAGRTTLNGEGLQHEDGHSHLMSATIPN
                     CVSYDPTFGYELAVIIQEGLRRMVQNQEDVYYYITLMNENYSHPAMPKGAEAGILKGM
                     YQFSQSKSKGPKVQLMGSGVILREVIAAAELLEKDWGVSADIWSVTSFTELRREGLDV
                     ERWNMLNPDKKQKQTYVGECLSKAEGPVIASTDYMKSFADQIRNFIPQRYVVLGTDGY
                     GRSDSREALRSFFEVDRYYVVLAALKALADEGKLPATKAAEAIKKYKIDVKRPNPTTV
                     "
BASE COUNT          892 a         1505 c         1635 g         1113 t
ORIGIN      
        1 ctttgtcgaa gatcagcttg gtgctgccct cggagcgcgc gattgagagt gcgcgaccgc
       61 tggctgccca ggggaaggaa gccttctcga tctcgatacc cttggccttg gcttcggtct
      121 cgctgacgcc tgcccaggcc acttcggggt cggtgtaggc gacggacggg atcgccagtg
      181 cctggaattc gaccttgtgg ccggcgatga cttcagcggc gaccttgcct tcatgcgtgg
      241 ccttgtgcgc cagcatgggt tggccgacga tgtcgccgat ggcgaagatg tgcgggacat
      301 tggtgcgcat ctgcttgtcg acggcgatga agccgcgctc gtcgaccgcg acgccagcca
      361 gttccgcgcc gatgttcttg ccgttggggc ggcggccgat ggcgaccagc acgcggtcat
      421 agacttgcgg ttcgatggcg ttctcgccct cgaaactgac gtggatgccg tccttcttcg
      481 cttcgatctt gctcaccttg gtggacagca tgatcttctc gaagcgtttt tccatgcgct
      541 tttgcagcgg acgcacgaga tctcggtcgc aaccggggat caatccgtcc atgaactcga
      601 cgacgctgac cttggagccg agcgcgtcat acaccgtgcc catttcaagg ccgatgatgc
      661 cgccgccgat gaccagcagg cgtttcggga tatcagccaa tgccaatgcg ccggtggaat
      721 ccatgatgcg ctcgtcttcc ggcgcgcctg ggaatttcgt cgcttgcgaa ccggcggcga
      781 tgatggcgtt gtcgaaggat acggtggtca ccttgccatc ggccgcggtc acggcgatct
      841 ggttcgggcc ggtgaacttg ccgacgccga ccaccgtggt gacttcacgc tgtttcgcca
      901 tctgggcgag gccgcctgtg agtttggcga cgacgtcgtt ggctttccag ttgcggagtt
      961 cttcgagttt gatctgtggc ttgccgaagc tgacgccgtg gtgggccgtc tcttccgctt
     1021 cggtgatgac cttggccgta tgcagcaatg cctttgaagg gatgcagccg acattgaggc
     1081 aaacgccgcc aagcgtggcg tagcgttcga tcagcacgac cttcttgcca agatccgcgg
     1141 cgcggaacgc ggcggtgtac ccgccggggc cgctgcccag cacgacgact tctgcatgca
     1201 ggtccgatgc gcccaccgcg gctggtgccg gagttggtgc agtgacagtg gcgggtttgg
     1261 tttcagcaac cggcaccggt gcggcagcgg gtgctgctgc cggagcaggt ttggcttcag
     1321 tggccgcttc ggcttcgacc agcaaaatca aagtgccctt ggcaaccttg tcgccaacct
     1381 tcactttcac ttccttgacc gtgccagcgt gcgaggatgg gatgtccatc gacgccttgt
     1441 cagattcgag ggtgatcagc gaatcttcct tggcgatgac gtcgccagcc ttgaccaata
     1501 cttcaatgac atcgacgctg tcgaaattac cgatatccgg caccaatact tctgttaatt
     1561 gactcatccg tattccttac agcaacaagc gacgcacgtc ggacagcatc atacgcagat
     1621 ggctcgtgaa acgtgcgcca tcggcaccgt cgatcacgcg gtggtcgtag gagagtgaga
     1681 gcggcaggat cagacgcggc tcaaaactct tgctcttggg atcgaatacc ggctggaagc
     1741 tggagcgcga tacgcccagg atcgccactt cagggcaatt gatgatcggc gtgaacatgg
     1801 tgccgccgat accgccaagg ctggagatgg tgaagcaacc accttgcatc tcctcgatct
     1861 tgagcttgcg ttcgcgggcc ttggcagaga gctcggccag gtcgcgcgcg atatcgagca
     1921 cgtccttctg gttgacgtca cgcaccacgg gcaccaccaa gccatccggc gtgtcgcagg
     1981 cgaagccgac atggaaatac tgcttcatga tcaggctctc gccatccggc gacagcgacg
     2041 cattgaagtt gggataagtg cgcagcgcgt tgaccactgc cttcatcagg aaagccagca
     2101 gcgtcagctt gacgccgcgc ttttcggctt cggccagcat ggacttgcgg aaatcctcga
     2161 ggtcggtgat gtcggcttcg tcgaattgcg tgacgtgtgg cgcggtgacc cagttgcggt
     2221 gcaggttggc gcccgacaac ttcttgatgc gagacagcgg tttgctctcg atctcgccga
     2281 actggctgaa atcgatgacc ggcatcggca gggtgctgag gccggcgcct gcagccatct
     2341 cgctgcgtgg tttggaaagc tcgctcttga cgaaggcttg cacatccgat tgcaggatac
     2401 ggtccttggg gccgctgcct tggaccagcg cgaggttgac gccgagttcg cgcgcgaact
     2461 tgcgcacaga agggctggcg tgggagcgtt tgccgccatt ggtgacgacg ctggcaccga
     2521 tcggatccgg cttgtgtgct ggcacgatcg gcttcggtgc ctcaggtaca ggatgggacg
     2581 gcgccgggat cgcatgttcc tgcaccgcgg tcactaccgg cggggccgtt ggcgcagggg
     2641 ccggtttggc ggcggtcgct tgctcttcag ccgcgtccac cgtcaggatc aacgtgccct
     2701 gggcgacctt gtcaccgacc ttgagtttga cttctttcac cgtgccgcca aaaggcgcag
     2761 ggatgtccat ggaggccttg tcggactcca gcgtgatcag cgagtcatcc ttggcgatgc
     2821 tgtcgccagc ctttaccagg acttcgatca catccacgct gtcgaaattg ccgatatcgg
     2881 ggacgagtac gtctttgatt gccatgttga atcgtctcct gattaaaccg tggttggatt
     2941 agggcgcttg acgtcgatct tgtacttctt gatcgcctcg gcggccttgg tggcaggcag
     3001 cttgccttcg tcagccagcg ccttcagcgc ggccagcacc acatagtagc ggtcgacttc
     3061 gaagaagctg cgcagcgctt cgcgcgagtc ggagcggccg taaccatccg tacccagcac
     3121 gacatagcgt tgcgggatga agttgcggat ctggtcggcg aacgacttca tgtaatcggt
     3181 cgaggcgatg accggaccct cggccttgct caggcattcg ccgacgtagg tctgcttctg
     3241 cttcttgtcc gggttcagca tgttccagcg ttcgacatcc aggccttcac ggcgcagctc
     3301 ggtgaagctg gtgacgctcc agatatcggc ggacacgccc cagtccttct ccagcagttc
     3361 ggcggctgcg atgacttcgc gcaggatgac gccgctgccc atgagctgga ccttggggcc
     3421 cttgctcttg gattggctga actggtacat gcccttgagg atgcctgcct cggcaccctt
     3481 cggcatggcc ggatggctat agttctcgtt catcagggtg atgtagtagt acacgtcttc
     3541 ctggttctgt accatgcgac gcagaccttc ctggatgatg acggcgagtt cgtaaccgaa
     3601 ggtcggatcg taggacacgc agttcgggat ggtcgccgac atcaggtggc tatggccgtc
     3661 ctcatgctgc aggccttcgc cattcagcgt ggtgcgaccg gcagtggcgc ccagcaggaa
     3721 gccgcgcgca cggctgtcgc cggcggccca ggcgaggtcg ccgatgcgct ggaaaccgaa
     3781 catcgaatag aagatgtaga acgggatcat ctgcgtgccg gtcacgctgt atgaggtcgc
     3841 ggcggcgatc cagctcgaga acgagccggc ttcgttgatg ccttcctcaa ggatctggcc
     3901 gtccttctgt tccttgtaga acatgacctg gtcagaatcc tgtggctcgt acaactggcc
     3961 gacggaagag tagatgccga gctggcggaa catgccttcc ataccgaagg tacgggcttc
     4021 gtccgggacg ataggcacga catacttgcc gagttgcttg tcgcgcacca gggtcgagag
     4081 gatgcgcacg aacgccatgg tggtggagat ctcgcgttcg ccggtggcac ccagcatgtt
     4141 ctcgaatgca gtgagttccg ggacggtcag ctcgttgccc ttgcggcggc gggacggcac
     4201 atagccgccc agcgcgttgc ggcgctcgcg catgtattgc atctcgggac tgtcgtcagc
     4261 cggcttgtag aaggacaggt tctcgacctg ttcgtccgtc agcggcaggt cgaaacgatc
     4321 gcggaacgcc ttcagcgagg cgatatccat gctcttctgc tggtgggtgg tgttgtggcc
     4381 ttcgccggct tcgcccatgc cgtagccctt gacggtcttc gccaggatga cggtcggctg
     4441 acccttgtgc gaggtcgcag cagcgtaagc tgcatagact ttgtgcggat cgtggccgcc
     4501 acggttgagg cgccagatat cttcatcgga catggcggcg accatatcct ttagctcggg
     4561 atacttgccg aagaagtgtt cgcgcgtata tgcgccgccc ttggccttga agttctggta
     4621 ttcgccgtcg acggcttctt ccatgcgttt cttgagcagg ccgtccttgt ccatggagag
     4681 caacggatcc cagtacgaac cccagatgac ttttagtacg ttccagccgg agccgcggaa
     4741 atcggcttcg agttcctgga tgatcttgcc gttgccgcgt actggaccgt cgaggcgctg
     4801 cagattgcag ttgatgacga agatcaggtt gtcgagcttt tcgcgtgccg ccagcgagat
     4861 tgcgcccagg gattccggtt catccatctc gccgtcgccg cagaacaccc agaccttgcg
     4921 gtcagaggta tcggcgatgc cacggtcgtg caggtacttg aggaagcgcg cctgatagat
     4981 gccctgtaag gggccgagac ccatggatac ggtggggaac tgccagaaat ccggcatcag
     5041 ccatgggtgt ggataggacg acaggccctt gccttccgtc tcgatacgga agttggacaa
     5101 ctgctcctcg gtcagccgac cttcgaggaa ggcgcgcgca taaac
//
LOCUS       DATMTW010000121         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_244925, whole genome shotgun sequence.
ACCESSION   DATMTW010000121 DATMTW010000000
VERSION     DATMTW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244925"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VN084_02275"
     CDS             <1..936
                     /locus_tag="VN084_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT28310.1"
                     /translation="GDMIAEDGIDILVDLAGYTTHARPEIMALRAAPIQIAYLGYPST
                     MGTDFIDYAIVDHIVAKPADQATAWSESLVRLPNAYCPYDHTMDHAPANLQRRDFGLP
                     DQAFVLCCFNNNYKIEPMVFAVWMRILTAVPEAVLWLLGLKADTQQNLLAAAEQAGIS
                     KERLVFAGVLPLTEHIKRYQLADLFLDTYWHNAHTTAADALWQGLPVLTCEGAVASSR
                     LASSLLHALDMQDELVTCNLDEYEQKAIHYAKNRNALKAVRDKLAANRESQPLFDTAL
                     TVQHLEQAYLAVWARHQAGLPPTGIDIADIRETVH"
     gene            933..>2081
                     /locus_tag="VN084_02280"
     CDS             933..>2081
                     /locus_tag="VN084_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWT28311.1"
                     /translation="MSDLFEPASPQAPLAEQLRPKTLDEVVGQSHLLGEGKPLRLAFT
                     SGKLPSMILWGPPGVGKTTLARLIANTADAEFIPLSAVLAGVKDIRDAVQLAEHTLQQ
                     SGRRTIMFVDEVHRFNKSQQDAFLPFVESGLITFIGATTENPSFEVNSALLSRAQVYV
                     LSALSEAELGQLLDRALAVAGQGVTLTAEVREQVLAYADGDARRLLNFAEGLFNAAQA
                     AGIETVDETFLQTTMASKLRRFDKGGEAFYDQISALHKSVRGSNPDAALYWFLRMIDG
                     GADPLYLGRRIVRMAVEDIGLADPRAQTMTLEACQIYERLGSPEGELALANAVIYLAV
                     APKSNAAYMAYNQAKAFVGSDKSRAVPLHLRNAPTKLMKALDYGKEYRY"
BASE COUNT          427 a          634 c          612 g          408 t
ORIGIN      
        1 ggtgacatga tcgcggagga cggcatcgat atcctggtcg atctcgctgg ctacaccacc
       61 cacgcacgcc cagagatcat ggcgctcaga gcggcaccga tccagatcgc ttaccttggt
      121 taccccagca ccatgggcac ggatttcatc gactacgcga tcgtcgatca tatcgtcgcc
      181 aaaccggccg atcaggcgac ggcatggagc gagtcacttg tgcgactgcc caatgcctat
      241 tgtccttatg accacacgat ggatcatgcg ccggcaaatc tgcagcgcag ggatttcgga
      301 ttgcctgacc aggcttttgt gctttgctgc ttcaataaca actacaagat cgagcccatg
      361 gtcttcgccg tctggatgcg tatcctcacg gcagtgcccg aagccgtgct ctggctactg
      421 gggttaaagg cagataccca gcagaacctg ctggccgcgg cggagcaagc gggcatctcg
      481 aaagagcgcc tcgtatttgc cggggtgttg ccgctgacag aacatatcaa gcgttaccag
      541 ctcgcggatt tgttcctcga cacctactgg cataatgcgc ataccacggc tgcggatgcc
      601 ttgtggcaag ggctgccggt attgacctgt gagggagccg tcgcgtcttc gcgcctggcg
      661 tcgtcgctgt tgcacgcgct ggacatgcag gacgaactcg tgacctgcaa tctcgatgaa
      721 tacgagcaaa aagcgatcca ttacgccaag aaccgcaacg cactaaaagc tgtgcgcgat
      781 aaactggcag ccaatcgtga aagccagccg ctgttcgata cagcgctgac cgtgcaacat
      841 cttgaacaag cctatctggc agtgtgggcc cgccatcagg ctggtctgcc gccaaccggg
      901 atcgatatcg cggatatcag agagacagta cattgagtga tctattcgaa cctgcgagcc
      961 cgcaggcccc actggctgaa cagctgcgcc ccaagacgct ggacgaggtc gtcgggcaat
     1021 cgcacctgct gggcgaaggc aagcctttgc gactggcgtt cacctccggc aagttgccgt
     1081 cgatgatcct ttgggggccg ccaggcgtcg gcaagacgac cttggcacgt ttgatcgcca
     1141 atacggcgga cgcggaattc atccccttgt cggctgtgct ggccggcgtc aaggatatcc
     1201 gggatgccgt gcaattggct gagcatacgc tgcagcaatc cggccgtcgc accatcatgt
     1261 tcgtcgacga agtgcatcgc ttcaataaat cgcagcagga tgccttcctg ccgttcgtcg
     1321 agagcggcct tatcactttc atcggcgcca ccaccgagaa cccctcattc gaagtcaaca
     1381 gtgcgctgct gagccgtgca caggtatatg tgctcagtgc cttgtccgaa gccgagcttg
     1441 gtcaactact cgatcgcgcg ttggcggtgg cagggcaggg tgtgaccctg acggcggaag
     1501 tgcgcgaaca ggtgctagcc tatgccgatg gcgacgcgcg gcgtttgctg aattttgccg
     1561 aaggcctgtt caatgccgcg caagccgcag ggatcgaaac ggtcgacgaa accttccttc
     1621 aaaccacgat ggccagcaag ctgcgccgct tcgacaaggg cggcgaagcc ttctacgacc
     1681 agatatcggc cttgcataaa tcggtgcgtg gttccaatcc cgatgccgcg ctctactggt
     1741 tcctgcgcat gatcgacggc ggcgccgatc cgctgtacct gggtcgccgc atcgtgcgca
     1801 tggcggtcga ggacatcggt ctcgccgacc cgcgcgcgca gaccatgacg ctggaagctt
     1861 gccagatcta cgagcggctg ggctcgcccg agggcgaact ggcgctggcg aatgccgtca
     1921 tctatcttgc tgtcgcgcct aagagcaacg ccgcttacat ggcctacaat caggccaagg
     1981 ctttcgtcgg tagcgacaaa tcgcgggccg tgccgctaca tctgcgcaat gcgccgacca
     2041 agctcatgaa ggccctggat tacggcaagg aataccgcta c
//
LOCUS       DATMTW010000122         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_614602, whole genome shotgun sequence.
ACCESSION   DATMTW010000122 DATMTW010000000
VERSION     DATMTW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614602"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VN084_02285"
     CDS             <1..998
                     /locus_tag="VN084_02285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWT28312.1"
                     /translation="AGLIVLGPVLIALGIGLFINGIQAFNHKTPDRHIPIAMALGTAI
                     IDTYFLLVNNDLRTVISFNSAVFSLAYVAAARMLFRQPVNRELKRFYNINSGLFLLMA
                     AAMAVRASAAMLASSVVFSQVADWPVYRLTFMAVIAIQLCVTFCFVLMLNYRQVFMLK
                     RIATHDVLTGILNRNGLMEAAARAAANCEKHGEPMTVLSIDIDGMAKINRQYSDFVGD
                     EALRELAEALTERARPTDLVGRYGNDEFCMVLPGTSEADAMQMAETIREGFNFKLILA
                     DDNHTNASVSIGVSSSEHMGLGFAELYAAAHSAMYNAKELGRNRVVAHSVIHQFN"
     gene            1121..2260
                     /locus_tag="VN084_02290"
     CDS             1121..2260
                     /locus_tag="VN084_02290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWT28313.1"
                     /translation="MVVVLTPLLSLLLILARVHADGVHGLGHWALGNLCISIGMLIMM
                     MQFKSTYFAILPATTLYALGLGLYLNGTQAFTEKRPTYGITAIIVAMVIVIDGVFLWK
                     QDFRIIVVFNAFIFMAVNIFGARMLLIPVKPPLRTAYWFTGSMFSLMALVFLIRGFGG
                     LIAEDAVFQAMSQWPLNKFTFFFGTVFQLCTTFGFILMLNYKLVERMRRMAGEDWLTG
                     ALNRRSLEDAANRMEANCRRFNLGIAMLLIDLDHFKQINDRYGHQFGDQVLAEFARIV
                     VGSIRAGDLFGRYGGEEFCILLPNTSEDEALTQADRLRQAFETDMTVFRNKRLACTIS
                     IGVSESTRVGHDFSALFAAADRALYEAKQRGRNRVVPYSALAGDA"
     gene            2291..>2993
                     /locus_tag="VN084_02295"
     CDS             2291..>2993
                     /locus_tag="VN084_02295"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HWT28314.1"
                     /translation="MAAWNEGRVVGQKQWNDKLFSIYVEADIEDYEAGQFAKLGLQVG
                     DEIIGRPYSLVNPPDRRPLEFYYIHVPDGPLTSALVKLQQGDRILVSPRANGFMILDE
                     VPQSRYLWLIATGTGVGPFLAILDTLKPWQRFEKVVLVYAVRSLSELSYQERIAGVQA
                     RHPERFVYVPFISREASDFAMPGRIPDAIVDHRLETRAGLLLNAEDTQVMMCGNPQMV
                     EDTMRVLVERGFKKHR"
BASE COUNT          567 a          922 c          883 g          621 t
ORIGIN      
        1 ttgccggcct gatcgtgctc ggccccgtgc tcatcgcctt gggcatcggt ctcttcatca
       61 acggtatcca ggccttcaac cacaagacgc cggaccgcca tatccccatc gccatggcac
      121 tcggcaccgc catcatcgac acttatttcc tgctggtgaa taacgacctg cgtacggtca
      181 tcagcttcaa ctcggcggtg ttctcgctcg cctatgtcgc agcggcacgc atgctcttcc
      241 ggcagccggt gaaccgcgag ctgaagcgct tctataacat caatagcggg ctgttcctgc
      301 tcatggcggc agccatggcg gtgcgtgcca gcgcggccat gctggccagc agcgtggtgt
      361 tctcgcaagt ggccgattgg ccggtctacc gcctgacctt catggcggtg atcgcgatcc
      421 agctctgtgt caccttctgt ttcgtgctca tgctcaacta ccggcaggtg ttcatgctga
      481 aacgcatcgc cacgcatgac gtgctcaccg gcatcctcaa ccgcaatggc ctgatggaag
      541 ctgcggctcg cgctgcggcg aactgcgaga agcatggcga gccgatgacg gtgttgtcga
      601 tcgatatcga cggcatggcg aagatcaacc ggcaatacag tgattttgtc ggcgacgaag
      661 cactgcgcga gctggctgaa gccctcaccg aacgtgcgcg gccgacggat ctggtcggcc
      721 gttacggcaa tgacgagttc tgcatggtgt tgccgggcac ttccgaggcc gacgccatgc
      781 aaatggccga aaccatccgc gaaggcttca acttcaagct gatcctggcg gacgacaatc
      841 ataccaacgc cagcgtcagc atcggcgtca gtagctccga gcacatgggg ctgggcttcg
      901 ccgaactcta tgcggccgcg cactctgcga tgtacaacgc caaggagctg ggccgcaatc
      961 gggtggtggc gcattccgtg atccaccagt tcaactgata cccccacatg cgacatgtgt
     1021 ttgcggatcg agatttatgg cagactcgac atgagtctaa acggggggcg ctgacgcgat
     1081 cttagccttg ttcgaactgg atttccgcac gatagtattc atggtcgtag tgctaacccc
     1141 gctactgtca ttgctattga tcctggcgcg cgtgcatgcc gatggtgtcc atgggctcgg
     1201 gcactgggca ctcggcaacc tctgcatcag catcggcatg ctgatcatga tgatgcagtt
     1261 caagagcacc tatttcgcca tcctgccggc gaccacactc tacgcgctcg ggctcgggct
     1321 ttacctcaac ggcacgcagg cgtttaccga aaaacggccg acctacggca tcaccgcaat
     1381 catcgtggcc atggtgatcg tcatcgatgg cgttttcctc tggaagcagg acttccgcat
     1441 catcgtcgtg ttcaatgcct ttatcttcat ggcggtcaat atcttcggtg cacgtatgct
     1501 gctgatcccg gtcaagccgc cgttacgcac cgcctactgg ttcacgggct ccatgttcag
     1561 cctcatggcg ctggtattct tgatccgggg ttttggcggg ctgatcgccg aggacgcggt
     1621 gttccaggcc atgtcccagt ggccgctgaa taaattcacc ttcttctttg gcacggtgtt
     1681 ccagctctgc acgacgttcg gtttcatcct catgctgaat tacaagctgg tcgagcgcat
     1741 gcgtcgcatg gcaggggagg actggctgac cggcgcgctc aaccggcgca gcctggagga
     1801 tgccgcaaac cgcatggaag ccaactgccg tcgcttcaac ctcggtatcg ccatgctgct
     1861 gatcgacctc gatcatttca agcagatcaa cgaccgctac ggacatcaat tcggcgacca
     1921 ggtgctggcc gagtttgccc gcatcgtcgt cggcagtatc cgggcgggcg acctgttcgg
     1981 ccgctatggc ggcgaggagt tctgcatcct tttgccgaac accagtgagg atgaggcgct
     2041 gacgcaggcc gatcgcttgc gtcaggcgtt cgagaccgat atgacggtat tccgcaacaa
     2101 acgcctggcc tgcaccatca gcatcggcgt cagcgaatcg acccgggtgg gccacgattt
     2161 cagcgcgctt ttcgccgctg ccgaccgcgc cttgtacgaa gccaagcaac gcggacgcaa
     2221 tcgtgttgtc ccatactcag cgttggccgg cgacgcatga acgccggtat cacggaaagc
     2281 gggtaaagcg atggcagcat ggaacgaggg ccgtgtggtc ggccagaagc aatggaacga
     2341 caaactcttc agcatctatg tcgaggcgga tatcgaggac tatgaagcgg gccagttcgc
     2401 caagctcggg ctgcaggtcg gcgacgagat catcggtcgg ccatactcgc tggtcaaccc
     2461 gccagaccgg aggccgctgg agttctatta catccacgta ccggacggcc cgctcaccag
     2521 cgcgctggtg aagttgcagc aaggcgatcg catcctggtc tcgccgcgcg cgaatggctt
     2581 catgatcctg gatgaagtcc cgcaaagccg atatctctgg cttatcgcca ccggcaccgg
     2641 cgtcggtccg ttcctcgcca ttctcgacac gctcaaaccc tggcagcgtt tcgagaaagt
     2701 ggtgctggtg tatgccgtgc gcagcttgtc cgaactgagt taccaggaac gcatcgcggg
     2761 ggtgcaggcc cgtcatccgg agcgcttcgt ctatgtgccg ttcatcagcc gcgaggcctc
     2821 ggattttgcg atgccggggc gcattcccga cgccatcgtc gaccaccggc tcgaaacccg
     2881 tgccggactg ctgctcaacg ctgaggatac ccaggtcatg atgtgcggca acccgcagat
     2941 ggtcgaggac acgatgcggg tgctggtcga gcgtgggttc aagaaacacc gcc
//
LOCUS       DATMTW010000123         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_278420, whole genome shotgun sequence.
ACCESSION   DATMTW010000123 DATMTW010000000
VERSION     DATMTW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278420"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VN084_02300"
     CDS             complement(<1..801)
                     /locus_tag="VN084_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWT28315.1"
                     /translation="MAVPASDHPALNWRKYLLDQQQQLRVRFMEKQDTSDLLRQHCRL
                     VDDLLRDLWKSAAIPDGFCLIAVGGYGRGELFPYSDVDLLILLPDDADSVHDQAIEAL
                     IGVLWDIGLAVGHSVRNLSECIEEAAKDVTVQTNLSEARLLIGSRKLYKTFGEAMRSA
                     MDPQRFFDAKLQEQQQRHAKFNDTSYNLEPNIKESPGGLRDLQSVLWIAHSMGFGNSW
                     QALVRHGLLTPLEARQIRRHERHLQTLRIRLHYLAKRREDRLLFDFQND"
     gene            complement(812..1609)
                     /gene="map"
                     /locus_tag="VN084_02305"
     CDS             complement(812..1609)
                     /gene="map"
                     /locus_tag="VN084_02305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWT28316.1"
                     /translation="MAITIKNATDIEKMRIAGKLASEVLDFITPHVKAGVTTGELDAL
                     CHDYMVNVQKTIPAPLHYTPPGHTPYPKSICTSVNHQICHGIPGDKVLKNSDIVNIDI
                     TVIKDGYHGDTSRMFYVGEPSIQARRLCEITYECMWLGIAKVKPGATLGDIGHAIQSY
                     AEKQGYSVVREFCGHGIGKKFHEDPQILHYGRPGAGMKLQAGMMFTVEPMINAGKRDI
                     KQLNDGWTIVTKDHSLSAQWEHTVLVTESGFEVMTVSAGSPPIPDFV"
BASE COUNT          346 a          497 c          523 g          401 t
ORIGIN      
        1 gtcgttctgg aaatcgaaca gcaggcgatc ctcacgtcgc ttcgccaggt agtgcaggcg
       61 gatacgcaag gtttgcaggt gccgctcgtg ccggcggatc tgccgggctt cgaggggcgt
      121 cagcaagccg tgtcggacca gtgcctgcca gctattgccg aagcccatgc tatgggcgat
      181 ccacagcact gactgcaggt cgcgcaagcc gcccggactc tccttgatgt tcggttccag
      241 attgtaggag gtgtcattga acttggcatg ccgttgttgc tgttcctgca gcttggcatc
      301 gaagaatcgc tgcggatcca tggcgctgcg catcgcttcg ccaaaagtct tgtataactt
      361 gcggctaccg atcagcagac gcgcttccga aagattggtc tggacagtca cgtccttggc
      421 ggcttcctcg atgcactcac tgaggttgcg cacgctgtgg ccgacggcaa ggccgatatc
      481 ccagagcacg ccgatcaggg cctcgatggc ctgatcatgg acgctatcgg catcgtccgg
      541 cagcaggatc aacaggtcaa cgtcggagta ggggaagagt tcgccgcgac cgtagccacc
      601 gaccgcgatc aggcagaaac catccgggat cgcggcgctt ttccagagat cccgtaacag
      661 gtcgtccaca agcctgcaat gctggcgcaa cagatccgac gtgtcctgct tttccatgaa
      721 acgcacacgc aactgttgtt gctggtcgag caggtatttg cgccaattca gcgctggatg
      781 atccgacgcg ggaacggcca taggtaactg cctagacgaa atccgggatg ggaggcgatc
      841 ctgccgagac ggtcatcact tcgaaaccgg actcggtgac gagaaccgta tgctcccatt
      901 gcgcggacag gctgtgatcc ttggtgacga tagtccatcc gtcgttgagc tgcttgatgt
      961 cgcgcttgcc agcgttaatc atgggttcga ccgtgaacat catgccagcc tgcagcttca
     1021 tgccggcgcc ggggcgaccg taatgcagga tctgcgggtc ttcgtggaat ttcttgccga
     1081 tgccgtggcc gcagaattcg cgcaccacac tgtaaccctg tttctcggca tagctctgga
     1141 tggcgtgacc gatatcccca agcgtggctc ccggcttgac tttggcgatg cctagccaca
     1201 tgcattcata ggtgatctcg cacaggcgcc tggcctggat cgacggctcg ccgacataga
     1261 acatgcggct ggtatcgccg tgataaccgt ccttgatgac cgtgatgtcg atattgacga
     1321 tatcgctatt cttcaacacc ttgtcgcccg ggatgccgtg gcagatttgg tgattgaccg
     1381 aagtgcagat ggacttgggg tagggcgtat ggcctggcgg cgtataatgt agcggtgccg
     1441 ggatggtctt ctgcacgttc accatgtagt cgtggcagag cgcgtcgagt tcacctgtcg
     1501 tcacgcccgc cttgacatgc ggtgtgatga aatcgagaac ctcggaagcc agtttgccgg
     1561 ctatgcgcat tttttcgata tctgtcgcgt ttttgatcgt gatcgccata gtgcatgatc
     1621 ggcccgaaag cccagttttt ccttgaatct caacagttgc ccatgttaac atacgtgccc
     1681 gtttcgcaga agcgattccc gcggggcgga acattatcaa tcttaaatcg agcatatgcc
     1741 ccgtacaggg tgtctggagc cagatct
//
LOCUS       DATMTW010000124         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_111770, whole genome shotgun sequence.
ACCESSION   DATMTW010000124 DATMTW010000000
VERSION     DATMTW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111770"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1073
                     /locus_tag="VN084_02310"
     CDS             15..1073
                     /locus_tag="VN084_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWT28317.1"
                     /translation="MLPDYRLVLLLAAALGLIYLLSPILAPFLIAATLAYICSPLVDR
                     LAGIRIGPCKINRTLGTLLVSAMIVGIVILVFLIIVPLLQKELLTVVERLPKYLATAK
                     ARIEPWLMAHFGVALAIDSDQIQQLVTKNWKSAGNLLAPLLVAVSNHGLALIGWVTNL
                     LLIPLVLFYLLRDWHALVGRIADLLPRRWYDKTAEIAGEIDRVLAEFLRGQLSVMLAM
                     SVFYAVGLWMAGLELAIPIGMIAGLLGFVPYLGIGIGILLAVLAGALQFNTLTEMIPV
                     AIVFGLGQVLESFVLTPWLVGDRIGLHPVAVIFALLAGGQLFGFTGVLLALPAGAAIA
                     VGLRHAKRHYQASDAYLK"
     gene            1070..1678
                     /locus_tag="VN084_02315"
     CDS             1070..1678
                     /locus_tag="VN084_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HWT28318.1"
                     /translation="MKQLLLDIQPAAQPSLANFVVGQNAEALDTLTRACEGKPEARFI
                     YLWGPPGSGKSHLLQACADAARQHGVALLVADDVHLLNEDDQIALFDAFNQLREGGGT
                     LISSGIASPLQMGLRDDLATRLAWGLVYQLQPLSDTEKAEALRKHAAERGMKLPEEVL
                     DYCLRHLRRDLPTLMATLDALDEWSLTDKRPVTLPLLRQLLR"
     gene            complement(1954..2271)
                     /gene="soxZ"
                     /locus_tag="VN084_02320"
     CDS             complement(1954..2271)
                     /gene="soxZ"
                     /locus_tag="VN084_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HWT28319.1"
                     /translation="MSDDIKMRAQRKGEITEVKVLMSHPMETGRRKNDLDETIPAHFV
                     QLVTATLNGQPVLEAQWGTGISKNPYLTFHLKDAKVGDKVAVTWHDNKGQSASHEIEV
                     VQG"
     gene            complement(2341..2763)
                     /locus_tag="VN084_02325"
     CDS             complement(2341..2763)
                     /locus_tag="VN084_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HWT28320.1"
                     /translation="MGLAGGLGVMLLMPLKALAAVWNREVFESTELAGAFKGLQAETQ
                     SPSQQIDLKAPDRAENGAVVQIEVSSRIPGTEAIAILVDHNPTPLIANFLFSQGTEPF
                     VVTRIKMAESSNVRAVAKVGDRYYEAMRFVEVLENGCN"
BASE COUNT          507 a          922 c          829 g          626 t
ORIGIN      
        1 gcaaacaaga aaacatcctg cctgactacc gcctcgtctt gcttctggcc gcagccctgg
       61 gtttgatcta cctgctatca cccatcctgg cgccttttct catcgccgcc accctcgcct
      121 acatctgcag ccccctggtc gaccggctgg ccggcatccg catcgggcct tgcaagatca
      181 accgcacctt gggcaccttg ctggtttcgg cgatgatcgt cggcatcgtc atcctggtct
      241 tcctcatcat cgtgccattg ctgcagaaag aattgttgac ggtggtggag cggctaccca
      301 agtatctcgc taccgccaaa gcacgcatcg aaccctggct gatggcccat tttggcgtcg
      361 cgttggcgat cgatagcgac cagatccagc aattagtcac caagaactgg aaatctgccg
      421 gcaacctgct ggcgccactg ctcgtagccg tgagcaatca cggcctggcc ctgatcggct
      481 gggtcaccaa cctgttgctg atcccgctgg tgctctttta cctgctgcgc gactggcatg
      541 cgctagtcgg caggatcgcc gacctgctgc cgcgccgctg gtatgacaag acagccgaga
      601 tcgccggcga gatcgaccgt gtgctggcag aattcctgcg tggccagctc tcggtgatgc
      661 tggcgatgag cgtgttctat gccgtcggcc tgtggatggc tgggctagaa ctcgcgatcc
      721 ccatcggcat gatcgccggt ctgctcgggt tcgtgcccta tctcggtatc ggtatcggca
      781 tcctgcttgc cgtgctcgcg ggcgccctgc agttcaacac cctgaccgaa atgatccccg
      841 tggccatcgt cttcggcctg ggccaggtgc tggagagttt cgtactcacg ccctggctgg
      901 tgggcgatcg tatcggcctc catccggtgg ccgtgatctt cgcgctgctg gcaggcgggc
      961 aattgttcgg cttcaccggc gtgttactgg cactccctgc cggcgccgcc atcgctgtcg
     1021 gcctgcgtca tgccaagcgc cattaccagg ccagcgacgc ttatctgaaa tgaagcagct
     1081 gctgttggat atccagccgg cggcgcagcc gtccctggcg aatttcgttg tcggccagaa
     1141 cgcggaagcg ctcgatacat tgacgcgggc ctgcgaaggc aagccagaag cgcggttcat
     1201 ctacctatgg ggcccgccgg gcagcggcaa gagccatctg ttgcaggcct gtgccgacgc
     1261 cgcccgtcag catggtgtgg cgctactcgt cgctgatgac gtgcacctgc taaatgagga
     1321 tgaccagatc gcgctgttcg acgccttcaa ccagctacgc gaaggcggtg gcacactcat
     1381 cagcagcggc atcgcctccc cactgcaaat gggcttgcgc gacgacctgg cgacgcgcct
     1441 ggcctggggg ctggtctacc agctacaacc gttgagcgac acggagaagg ccgaagcatt
     1501 gaggaagcat gccgcggaac gcggcatgaa gctgccggag gaagtgctgg attattgcct
     1561 gcgccacctg cgccgcgacc tgccgacact gatggcgacc ctggatgccc tggacgaatg
     1621 gtcgctgacg gacaagcgtc ctgtgacgct gcccttgctc aggcaattac tgcgctgatc
     1681 gctgatcgcc gatctctacg ctagaccact agaccatcag atccgcgcta accgcggtta
     1741 gtcgaatcta cggtaagtac gggcttactt gagcttcttg gcgatccagt acaggatcgc
     1801 accgaagaca atgatgatgc cgatcagggt gtagataccc tgccaacctt gaaagaacat
     1861 ggccatcctc cttcctcggc caaacggcaa gaccgtctgg cattgatcgg gatgtgtcgc
     1921 agcgccgctg cttcagcgct tgcggggtta accctagccc tgcaccacct cgatctcgtg
     1981 acttgcgctc tggcccttgt tgtcatgcca ggtcacggcc accttgtcgc caaccttggc
     2041 gtccttgaga tgaaaggtga ggtaggggtt cttcgagata ccagtacccc actgcgcctc
     2101 cagcaccggc tggccgttaa gcgtggccgt caccagttgc acgaaatggg cggggatcgt
     2161 ttcgtcgagg tcgttcttgc gccgcccagt ctccatcggg tggctcatga gcaccttcac
     2221 ctcagtgatc tcgcccttgc gctgcgcgcg catctttatg tcatccgaca attccatccc
     2281 tcctgttgat gcccacggcc tttaccgtgg ccatgttcga tatgcatgcg ctggcaggca
     2341 tcagttgcac ccgttctcca ggacttcaac gaaacgcatg gcttcataat aacgatcgcc
     2401 gaccttggcc acggcacgca cattggaact ttccgccatc ttgatacggg tcacgacaaa
     2461 gggttccgta ccctgactga agaggaaatt ggcgatcagc ggcgtcgggt tgtgatcgac
     2521 caggatggcg atcgcctctg tcccggggat acggctcgat acttcgatct gaaccactgc
     2581 gccgttctcg gcccgatccg gcgccttgag atcgatctgc tggctcggcg actgggtttc
     2641 ggcctgcagg cccttgaatg cacctgccag ctcggtcgat tcgaagacct cccggttcca
     2701 gacggcggcc agggccttga gcggcatgag cagcatcacg ccaagcccgc cggcgagtcc
     2761 catcaaaaat tccctgcgtt gcatctttgt cccttttcgt cgcagctagt tgaaccagcc
     2821 ttccttcttg ggtttgtcca cgcgctgctt gagttcgttc aatctcgctt cgacgatgga
     2881 ttgc
//
LOCUS       DATMTW010000125         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_212273, whole genome shotgun sequence.
ACCESSION   DATMTW010000125 DATMTW010000000
VERSION     DATMTW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212273"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..543
                     /locus_tag="VN084_02330"
     CDS             172..543
                     /locus_tag="VN084_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28321.1"
                     /translation="MNQHLQALTLSLALAALLAGCSSMDKYNPFTDTPVYQEISRAPA
                     DATEYRCEGEKKFYVRMLDKGSDAWLIYPDREVRLEKAGGSGNRYTNGVAVLDINGDQ
                     SSLSDGPQIDYKACKAANPKK"
     gene            545..1084
                     /locus_tag="VN084_02335"
     CDS             545..1084
                     /locus_tag="VN084_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWT28322.1"
                     /translation="MDASAADLRRLTLQFPYAGKVDAIYLRPARRVPAVAAQEAFAEA
                     GAGLVGDRKSLTVSGLPHGSKRQVTLLQAEHLPVIAALLKRSAIDPALLRRNLVISGL
                     NLLAARSLFKDRPLQLRVGACVLEITGPCEPCSRMEEVLGTGGYNVMRGHGGLNARII
                     ERGEIRVGDAVTCVALPAG"
     gene            complement(1094..2101)
                     /locus_tag="VN084_02340"
     CDS             complement(1094..2101)
                     /locus_tag="VN084_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWT28323.1"
                     /translation="MRAIFLLGLGLLATQNVLAEPSVDTVYYLKGSVVKVNTVTNKGG
                     QGVGSGVVVAENTVATNCHVLADSNGINITASGEFYLPTAVQEDWRHDVCILRFQFLP
                     LKPAVLGDSDQLQYEQSVFSIGFPGGPPKPLTTYGQIKALYPMDGSHVIRTSASFVMG
                     ASGSPIFDDEGKLLGMNTVKSPGRNAYFYGVPVNWIKQAMTLPQNPLISQPGTPFWDA
                     PEDQRPYFMRVVLPLQEEAWSELQQIAQAWLKQEPDNLEARYYVALSSEHLGHAAEAR
                     AGYEAILARNSRHTASLVGLGRLAAQAGDQPEVARLSLLLGDLDQEALETLKTQTGKQ
                     P"
     gene            2142..2849
                     /locus_tag="VN084_02345"
     CDS             2142..2849
                     /locus_tag="VN084_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWT28324.1"
                     /translation="MTRIPFTGQALQRVLRLLLATVLLLCATEALADRPPGFVYLDEA
                     IPTIRLEMRYASNDNFIGRPIDGYQKPRALLTAEAALALLKVQDALADFKLGLKVYDA
                     YRPQRAVNDFLRWAADPADTRQRAEYYPEVEKPDLFKQGYLAERSGHSRGSTVDLTIV
                     ELDSGKELDMGGSYDYFGPLSWPTSTLPTPQQRASRMLLQSVMLRFGFVPYPQEWWHF
                     TLKDEPHPDQYFDFPVE"
     gene            complement(2904..3149)
                     /locus_tag="VN084_02350"
     CDS             complement(2904..3149)
                     /locus_tag="VN084_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28325.1"
                     /translation="MTHIRFILIALPLMLLSACATPAGKADTRQWTTLTCSGFLQWKD
                     CWDQARAICPGGFDIANQIEMRGQQKREVEIACKAGR"
     gene            3323..>3762
                     /locus_tag="VN084_02355"
     CDS             3323..>3762
                     /locus_tag="VN084_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWT28326.1"
                     /translation="MLIQSHIVDLPTPTGVMRTYVHRPVAEGRYAAILFYSEIFQQTG
                     PIERAAKIMAGHGYAVLVPEVFHELNPIGTVLGYDDAGREKGNADKAAKDVQGYDSDN
                     RAMIEWVQAQPWCSGKVGAMGFCIGGHLAFRAALQPEVKATACF"
BASE COUNT          720 a         1189 c         1148 g          705 t
ORIGIN      
        1 atggacacct cccgtggtcg tctcgtcact caggagacgg tcaaaagtac acatggcaaa
       61 acaattcccc gaaccggttc gaagccgatc acgccggcaa agttcctaag ttggttgagc
      121 cgaacggact agtttggcaa gataggggtc tttggcacaa tggaacggta catgaaccag
      181 catttacaag cattgaccct gtcgctggcc ttggcggccc tgctcgcagg ctgcagctcg
      241 atggacaagt acaatccctt caccgatacg cctgtctatc aggagatctc gcgtgcgccg
      301 gccgacgcca ccgaataccg ctgcgaaggg gagaaaaagt tctatgtgcg tatgctggac
      361 aagggcagcg acgcttggct catctacccg gatcgggaag tacgcctgga aaaggccggt
      421 ggtagcggca accgctacac caacggtgtg gccgtgctgg acatcaatgg ggaccagagc
      481 agcctgagcg acggcccaca gatcgattac aaggcctgca aggccgctaa ccccaagaaa
      541 tagcgtggat gcgagcgcgg ccgacctgcg ccgcctgacc ctgcagttcc cttatgccgg
      601 caaggtcgac gccatctatc tgcggccggc acgtcgggtg ccggcggtcg ccgcacaaga
      661 ggctttcgcc gaagccggcg ccggcctggt cggcgatcgc aaatccctca cagtgtctgg
      721 cctgccgcat ggcagcaagc gccaggtgac gctgttacag gccgaacact tgccagtgat
      781 cgccgccttg ctaaagcgta gcgcgatcga ccctgccctg ttgcgccgaa acctcgtcat
      841 ttccggcctc aatctgctgg ccgcccgcag cctgttcaag gatcgcccgc tgcaactgcg
      901 ggtcggtgcc tgcgtgcttg agatcaccgg accgtgcgag ccgtgttcgc gcatggaaga
      961 ggtgctcggg acaggcggct acaacgtcat gcgcggccac ggcggactca acgcgcgcat
     1021 catcgagcgc ggcgagatcc gcgtcggtga cgcggtcact tgcgtggcgc tgcctgcggg
     1081 ctgagcgctg gcctcagggc tgcttaccgg tttgcgtttt caaggtttcc agcgcttcct
     1141 gatcgagatc cccaagcaac agactgagcc gcgcgacctc cggctgatcg cctgcctgcg
     1201 cggccagtcg tcccagcccg accaggcttg ccgtatgccg gctattgcgc gcgaggatcg
     1261 cctcatatcc cgccctggcc tcggcggcat ggcctagatg ttcggacgac agcgcgacgt
     1321 aatagcgggc ttcgaggttg tctggctcct gcttgagcca ggcttgcgcg atctgttgca
     1381 actcgctcca ggcttcttcc tgcaagggca gtaccacgcg catgaaatag gggcgctggt
     1441 cttccggcgc atcccagaag ggcgtgcctg gctggctgat gagggggttc tgcggtagcg
     1501 tcatggcctg cttgatccag ttgaccggca cgccgtagaa ataggcgttg cggcccgggc
     1561 tcttgaccgt attcatgccg agcagcttgc cctcgtcgtc gaagatcggg ctgccgctgg
     1621 cgcccatgac gaacgaggcc gaggtacgga tgacgtggct gccgtccatc gggtatagcg
     1681 ccttgatctg gccgtaggtg gtcaacggct tgggcgggcc gccgggaaag ccgatcgaga
     1741 acaccgattg ttcgtattgc agctgatcgc tgtcgccgag cacggctggc ttcaagggca
     1801 ggaactggaa gcgcaggatg cagacgtcgt gacgccagtc ctcctgcacg gcggtcggca
     1861 ggtagaattc gccggaagcg gtgatgttga tgccgttgga atcggccagc acatggcaat
     1921 tggtggcgac cgtgttctcg gcgaccacga ctcccgagcc gacgccctgg ccccccttgt
     1981 tggtgacggt attgaccttg acgaccgagc ccttgaggta atagaccgta tcgacactgg
     2041 gctccgccag gacgttctgg gtggccagta gccctagccc gagcagaaag attgcgcgca
     2101 taatgttccc ttcaagacac atcctaaaca gaccagatcc catgacaagg attcccttta
     2161 ccggacaggc tttgcagcgc gtgcttcgcc tgctgctggc taccgtattg ttgctttgcg
     2221 ccacagaagc gctggccgac cggccgccag gcttcgttta cctcgacgag gctatcccca
     2281 ccatccgtct cgagatgcgc tacgcctcca acgacaattt catcggccgc cccatcgacg
     2341 gataccagaa accgcgtgcc ctgctgacgg cagaggccgc gctggccttg ctcaaggtgc
     2401 aggacgcgct cgccgacttc aagctcgggc tcaaggtcta cgacgcctac cgcccgcagc
     2461 gggcggtgaa cgacttcctg cgctgggctg cggatcctgc cgataccagg caacgcgccg
     2521 aatactaccc ggaggtcgag aaaccggacc tcttcaagca aggttacctc gccgaacgtt
     2581 ccgggcatag ccgcggcagc acggtcgacc tgaccatcgt cgaactcgac agcggaaagg
     2641 aactcgatat gggcggcagc tacgattatt tcggcccgct ttcctggccg acgtccacct
     2701 tgccaacccc gcaacaacgc gcgagccgca tgttgctgca atcggtgatg ctacgtttcg
     2761 gcttcgtgcc ctatccgcag gagtggtggc atttcaccct gaaagacgaa ccccaccccg
     2821 accagtattt cgactttccc gtcgaatgaa cgaacggcgg cattagctgc gccagccagt
     2881 gccagcgggc gatcagccca ggactagcgg cccgctttgc aggcgatctc gacttcgcgc
     2941 ttctgctgtc cgcgcatctc gatctggttg gcgatatcga acccacccgg gcagatcgca
     3001 cgcgcctggt cccagcagtc cttccattgc aggaagccgc tgcaagtgag cgtggtccac
     3061 tgacgggtgt cagccttgcc ggcaggcgtg gcgcaagccg acagcagcat cagcggcagg
     3121 gcgatgagga taaaccggat atgcgtcatg atagcgatcc tgcgagttgg gtcagttcta
     3181 cgactatgca cggaccctga aggttcagtg gcggcgcagg cgaagcatct ccacataaat
     3241 ttaccgtgag ctgcgctgcg cttcgagcta aaatgtctgc ctaatcaagt cacttacaaa
     3301 aaccgatcgc gcgaggagcc ccatgctgat tcaaagccat atcgtagatt tgcccacccc
     3361 taccggcgtc atgcgcacct atgtccatcg cccggtggcc gaaggccgtt atgcggccat
     3421 cctgttctac tccgagatct tccagcagac cggtccgatc gagcgcgccg ccaagatcat
     3481 ggctggccac ggctacgccg tcctggtgcc ggaagtgttc cacgagctga acccgatcgg
     3541 taccgtcctc ggctatgacg atgccggccg cgagaaaggt aatgccgata aggcggccaa
     3601 ggacgtgcag ggttacgaca gcgacaaccg cgccatgatc gagtgggtgc aggcccagcc
     3661 atggtgctcc ggcaaggtcg gcgcaatggg cttctgcatc ggtggccatc tggcgttccg
     3721 tgccgcgttg cagcccgaag tgaaggccac ggcctgtttc ta
//
LOCUS       DATMTW010000126         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_615010, whole genome shotgun sequence.
ACCESSION   DATMTW010000126 DATMTW010000000
VERSION     DATMTW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615010"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..1123)
                     /locus_tag="VN084_02360"
     CDS             complement(353..1123)
                     /locus_tag="VN084_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWT28327.1"
                     /translation="MGTRRNRTINLPAPYLKRIWFDPSRVSDRSAYPLCLPFLQDDFE
                     LSFDRAITIIVGENGAGKSTLLEGIAVLAGYDEAGGGKGYRPVDHTDATEVMGGQLSN
                     AIRASWLPKITNGWFFRAESFFSVARYLDQAALEPLSGPPPDFLSHSHGEGFLRFFEE
                     RCQKQGIFIFDEPESALSPSRQIEFLKLLQRMENSRISQVIMATHAPMLMAYPGARLL
                     RMTKYGLEPVTIEQTDHYRVMREFCADPAGFIETVISE"
     gene            complement(1358..1750)
                     /locus_tag="VN084_02365"
     CDS             complement(1358..1750)
                     /locus_tag="VN084_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HWT28328.1"
                     /translation="MFSNQFFNVHRTWEDWLGMVLGLLIAVSPMLAGGEVSRAAIWNT
                     VLVGVLIFFIAELEYAVLQRWEEICQLVLGLWLIASPYVLGYSAAGSLRFWHSTLGGL
                     VILLAALELWQDWNRSDQDMVRRGKLFG"
BASE COUNT          423 a          577 c          533 g          358 t
ORIGIN      
        1 gatctgggga gaacttgtct tcagtgatga ctccgacgac gcggccagtc atgccgtgga
       61 ttcgaatccg gtctccgatc tccatccctc gcaaaaccga cattcctcag cctcccctgc
      121 gcagcttgcc gctaattatt agccgatctg agcgacggcc gattggcagt aaactctcgc
      181 gcggcaagcc cgtagcccgg atgcagcgca gcgaaatccg ggaacggcct gcctcgcact
      241 tcgctgcgct ccatgcgggc tacttatgga cgtgagaaga ccggtggccc aaagcgcaag
      301 ccgaagctgc ggagcgagta ccggctgttg aatagttctg cagactacac actcattcgc
      361 tgatcaccgt ttcaataaag ccagccgggt cagcgcaaaa ctcgcgcatg acccgatagt
      421 ggtctgtttg ctcgatcgtc actggctcaa ggccgtattt tgtcatgcgc agaagtcgtg
      481 cacctggata tgccatcagc attggcgcat gagttgccat gatgacctgg ctgatgcgcg
      541 aattctccat gcgctgtaac agtttcagga attcgatctg gcgagaaggc gaaagcgccg
      601 actccggttc gtcgaaaatg aaaataccct gcttctggca gcgttcctca aagaagcgca
      661 gaaatccttc gccatgcgag tgtgaaagaa agtctggcgg cggcccgctg agcggttcta
      721 aagcggcctg atcgagataa cgagcgacgg agaagaagct ttcggcgcga aagaaccagc
      781 cgtttgtgat tttgggcaac cagcttgcgc gaatggcatt cgaaagttgt ccccccatca
      841 cctcagtcgc atcagtatga tcgacgggtc ggtagccctt gcctccgccg gcctcgtcat
      901 acccggccag aacggcaatt ccttctagca aggtcgactt tcctgctcca ttttcaccaa
      961 cgatgatggt gatggcccga tcaaaactga gctcaaaatc atcctgcaga aatgggagac
     1021 agagcggata cgcagagcgg tcggaaactc gtgagggatc gaaccatatt cgcttgaggt
     1081 aaggggcagg tagattgatc gtacgattgc gtctagtgcc catgcgctat ccaatgccgg
     1141 ccgtcatcca acgcgcgtga gttttcggca ttttgacaga cgatgcaatg gcccgacgct
     1201 agggcgcgca gccaccgtag cccggatgca gcgcagcgaa atccggaaag cggcctgccc
     1261 cgcatttcgc ggtgctccat gctggctaca aaccgcggag acacattcat gcaacatcag
     1321 ccgccgacca gacggctgca ctcgtccctc gtctcactca cccgaacagc ttgccgcgtc
     1381 ggaccatgtc ctggtcgctt cgattccagt cctgccagag ttcaagcgct gcgagcagga
     1441 tgacgagacc gccgagcgtc gaatgccaga agcgcaggct gccggcggcg gagtaaccga
     1501 gcacgtaggg cgacgcgatc agccacagac cgagcaccag ttggcagatc tcctcccagc
     1561 gctgcaacac cgcgtattca agctcggcga tgaagaatat cagcacgccg accaggacgg
     1621 tattccagat cgccgcgcgg ctgacttcgc cgccggcgag catgggcgat accgcgataa
     1681 gcaggcccag caccatgccg agccaatctt cccaggttcg atggacgttg aagaattgat
     1741 tgctgaacat cgaagcctcc ctcgcaaaag aggcatacgt ctgcggctgg cagccactcc
     1801 gctccgaccg ttcgacgagc aacccagccg cgtcggggcg tatgtccaat aatcacgtat
     1861 gtggcgcgcg gaccgccgca agcccctgcc c
//
LOCUS       DATMTW010000127         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_581431, whole genome shotgun sequence.
ACCESSION   DATMTW010000127 DATMTW010000000
VERSION     DATMTW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581431"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VN084_02370"
     CDS             <1..888
                     /locus_tag="VN084_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWT28329.1"
                     /translation="AMAALESALESERASAESFKAAEARYKAGIATPADKLQAQTAYA
                     QTTLTRITAEGTLKTAYGNLANVMGLAANQQLTLARSQAIESQPSVEADIDALIDQAR
                     QRRPDLLASEAQVKAAEATIEASRAAAKPTVSLTASNSYQDGSSLTANNNSTLGISVS
                     IPLFSGYAPSYRIRSAEAAAEVRAAQRDRIRLQISLDVWRAYQSLRTANQSISAATTF
                     VSSAEESARVALGRYKAGVGNILDTLNAQSALATARQQKIQADLNWNIARATLAQSIG
                     VLDNAMIQSLPENNNFAMP"
     gene            919..>1521
                     /locus_tag="VN084_02375"
     CDS             919..>1521
                     /locus_tag="VN084_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWT28330.1"
                     /translation="MRHAIRKFSTLFVLVAIAIGGYYFYAQSKKPKPEEIYRQQAIKQ
                     GNVEQSVSANGTLNPVTIVSVGTQVSGRVSKLYVDFNDKVEKGQVLLELDDSLFAAQI
                     AQSSATVRNAQASLELAQANEARIRSLYQQEYVSRQELDQAVQALKSAKAQLDTASAQ
                     LRRDQTNLGYSIIRSPVSGVVVDRVIDVGQTVAASFQTPTL"
BASE COUNT          337 a          516 c          430 g          238 t
ORIGIN      
        1 gcgatggccg ccctggaatc cgccctggaa tcggagcgcg ccagcgccga gagcttcaag
       61 gctgccgaag cccgctacaa ggcagggatc gctacaccag ccgacaagtt gcaggcgcag
      121 acggcttacg cccagacgac actgacgcgg atcactgcgg aaggtacatt gaaaaccgca
      181 tacggtaacc tggcgaacgt gatgggtctt gccgccaatc agcagctgac actcgcacga
      241 agccaggcca tcgaaagcca gcctagcgtg gaggccgata tcgacgcgct gatcgaccag
      301 gctcgccagc gccggccgga tctgctggcc agcgaagccc aggtcaaggc cgctgaagca
      361 accatcgaag ccagccgtgc cgccgccaaa ccgaccgtct cactcaccgc gtcgaatagc
      421 taccaggatg gcagcagcct gacggcgaac aacaactcca cactcggcat cagcgtttcc
      481 atcccgctat tctcgggtta cgcgcccagt taccgcatcc gttctgccga agccgcggcc
      541 gaagtgcgcg cggcgcaacg cgaccgcatc cgcttgcaga tctcgctcga cgtctggcgc
      601 gcataccaga gcctgcgtac tgccaaccag tcgatctcag ccgccaccac gttcgtcagc
      661 agcgccgaag aatcggctcg tgtcgcttta ggtcgctaca aggcaggggt cggcaacatc
      721 ctggataccc tcaacgcaca gagcgcgctg gccaccgctc gccagcagaa gatccaggca
      781 gacctcaact ggaacatcgc gcgcgccacc ctggcacaat ccatcggcgt gctggacaac
      841 gccatgatcc agtcattgcc cgagaacaac aatttcgcca tgccctaggc caggcgcctc
      901 aacggaaaag acatcgacat gcgtcatgcc atcagaaaat tcagcacact gttcgtacta
      961 gtggccatcg ccatcggcgg ctactacttc tatgcgcaaa gcaagaaacc gaagcccgag
     1021 gagatctacc gccagcaggc catcaagcag ggcaacgtcg agcagtcggt gtctgccaac
     1081 ggtactctga atcccgtgac catcgtcagc gtcggtaccc aggtctccgg ccgtgtcagc
     1141 aagctctatg tcgacttcaa cgacaaagtc gaaaaaggtc aggtgctgct ggaactcgac
     1201 gactcgctgt ttgccgccca gatcgcgcaa tcttccgcca ccgtgcgcaa tgcccaggct
     1261 tcgctggagc ttgcgcaggc gaacgaagcg cggatccgct cgctctacca gcaggaatac
     1321 gtatcgaggc aggagctgga ccaggccgtg caggcgctga agtctgccaa ggcacagctg
     1381 gataccgcca gcgcgcagtt gcgtcgggat cagaccaacc tcggttactc catcatccgc
     1441 tcgccggtct ccggcgtggt ggtcgaccgg gtgatcgatg tcggccagac cgtcgccgcc
     1501 agtttccaga cgccgacgct g
//
LOCUS       DATMTW010000128         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_547644, whole genome shotgun sequence.
ACCESSION   DATMTW010000128 DATMTW010000000
VERSION     DATMTW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547644"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /gene="pheA"
                     /locus_tag="VN084_02380"
     CDS             complement(<1..597)
                     /gene="pheA"
                     /locus_tag="VN084_02380"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWT28331.1"
                     /translation="MSDQLKQCRDQIDAIDEQLLELVNQRAQLAREIGNLKGGGPIYR
                     PEREAQVLRRLLDHNAGPLSGEAITAIFRSVMSNCRALEKELSVAFLGPMGTFSEEAA
                     NKQFGGLTSPVQCASIDDVFRMVESGQVDYGVVPVENSTEGAVGRTLDLLMATSLHIC
                     GEVTLPVHHNLLSKAADKAAVRKVYSHAQSLAQCHEWLN"
     gene            complement(594..1676)
                     /gene="serC"
                     /locus_tag="VN084_02385"
     CDS             complement(594..1676)
                     /gene="serC"
                     /locus_tag="VN084_02385"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HWT28332.1"
                     /translation="MTQIYNFSAGPAVLPKVVLEQARDELLDWHGSGMSVMEMSHRGK
                     AFLGIAAEAEADLRELMAIPANYKVLFLQGGAHSQFSMIPMNLLRGRTRADYLDSGLW
                     SVKAIEEARRYCEVNVVASSRDRNYTYAPAQDQWRLDRDAAYVHYTSNETIGGVEIFW
                     TPDTGDVPLVADMSSHILSRPIDVSKFGLIYAGAQKNIGPAGLTIVIVRDDLIGQTLP
                     GTPTMFDYKTHADNDSMYNTPPTYGIYIAGLVFKWLKAMGGLSAMEKTNILKADMLYD
                     YLDSTDFYEAPVARENRSRMNVPFRIADASLDATFLAHAAEHGLLQLKGHKLVGGMRA
                     SIYNAMPVEGVATLVGFMKDFERAHA"
     gene            complement(1688..>4232)
                     /gene="gyrA"
                     /locus_tag="VN084_02390"
     CDS             complement(1688..>4232)
                     /gene="gyrA"
                     /locus_tag="VN084_02390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWT28333.1"
                     /translation="LAKEIIPVKLEDEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRR
                     VLFAMHELSNDWNRAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYMLVDG
                     QGNFGSVDGDNAAAMRYTEIRMARIAHELLADIDKETVDFGPNYDGSENEPLIMPARI
                     PNLLINGSSGIAVGMATNIPPHNLGEVIDACLTLLKYPETTVDELIDIIPAPDFPTAG
                     IIYGTVGVKEGYRTGRGRVVMRGRTHVEDMEKGNRQALIIDELPYQVNKKSLIEKIAE
                     LVNDKKIEGISDLRDESDKSGMRVVIELKRGEVPEVILNNLYKQTQLQDTFGMNMVAL
                     LDGQPRLLNLKQMLEAFLRHRREVITRRTVFELRKARERGHVLEGLAVALANVDEMIA
                     LIKAAPTPPDAKRELMARTWTSPVVEEMLKRAAMEASRPDGLSVDFGMTPKGYRLSDV
                     QAQEILQMRLQRLTGLEQDKIVNEYKEVMDVIADLLDILAKSERVTAIIVEELSAIRQ
                     QFGDKRRSEIIQNAQDLSLEDLITPQDVVVTLSHTGYIKSQPLDEYRAQRRGGRGKQA
                     TATKEDDFIDKMFVANTHDYILCFSSRGRVYWLKVYEVPQGSRISRGKPIVNLFPLEE
                     GEKINAILPVKEFDENHYIFMATSMGTVKKTSLVEFSNPRKSGIIAINLDDNDFLIGA
                     EITNGANDVVLVSNAGKAVWFDEEDVRPMGRNTRGVRGMKLQAKQQVISLLIADNEQQ
                     TVLVATENGYGKRTALADFRHSGRGTQGVKAIVTSERNGTVVAARLVGDDDEIMLITT
                     GGVLIRTRVKEIRELGRATQGVTLINLGEGEKLSGLEKVVETDDEQDEQ"
BASE COUNT          793 a         1273 c         1231 g          935 t
ORIGIN      
        1 attgagccat tcatggcact gggccagcga ttgtgcgtgt gaatagacct tacgcacggc
       61 tgctttgtcc gcggccttcg acaacaggtt gtgatgcacc ggcagcgtga cttcgccgca
      121 gatatgcaga ctggtcgcca tcaagagatc aagcgtgcga cccacggcgc cttctgtgga
      181 attctccacc ggaaccacgc catagtcgac ctgacctgat tcgaccatac ggaacacgtc
      241 gtcgatggaa gcgcattgca caggtgatgt cagcccaccg aactgcttgt tggcggcttc
      301 ttcactgaag gtgcccatag ggccgagaaa ggcgacggag agctctttct ccagtgcgcg
      361 gcaattggac atgacgctgc ggaagatcgc cgtgatggct tcgccagaca acggtcctgc
      421 attgtggtcg agcaggcggc gcagcacctg cgcctcgcgc tcggggcggt agatggggcc
      481 gccgcccttg aggttgccga tctctcgcgc cagctgcgcg cgctggttga ccagctctag
      541 caactgctca tcgatcgcat cgatctggtc acggcattgt ttgagttgat cgctcatgca
      601 tgcgcccgtt cgaaatcttt catgaacccg accaaggtgg ccacgccttc caccggcatc
      661 gcgttataga tcgatgcgcg catgccaccc accagcttat ggcccttcaa ttgcagcagg
      721 ccatgctctg cagcatgcgc caggaacgtc gcatcgagcg acgcgtcagc gatgcggaac
      781 gggacgttca tacgcgagcg gttctctctt gcgaccggcg cctcgtagaa atcagtactg
      841 tcgagataat cgtacagcat gtccgctttc aggatgttgg ttttttccat ggcagacaaa
      901 ccacccatgg ccttgagcca cttgaatacc agaccagcga tgtagatgcc gtaggtcggc
      961 ggcgtgttgt acatggaatc gttgtctgcg tgggtcttgt aatcgaacat cgtcggcgtg
     1021 ccgggtagcg tctggccgat gaggtcgtcc cgcacgatga cgatggtgag cccggccggc
     1081 ccgatgttct tctgcgcgcc ggcatagatc aggccgaact tgctgacgtc gatcggacgc
     1141 gacaggatat gggaggacat gtcggccacc aacgggacat cccccgtgtc gggcgtccag
     1201 aagatctcga cgccgccaat ggtctcgttg gaagtgtagt gtacataagc agcgtcacga
     1261 tcgagacgcc actgatcctg cgctggcgca taggtgtaat tgcggtcacg ggatgaagcc
     1321 accacgttga cctcacaata acgtctggcc tcctcgatcg cttttaccga ccacaggccg
     1381 gaatcgagat aatcggctcg cgtcctgccg cgcagcagat tcatggggat catggagaac
     1441 tggctgtgtg ccccaccctg caggaaaagc accttgtaat tggcagggat cgccatcaat
     1501 tcgcgcagat cggcctcggc ctctgccgca atacccaaga acgccttgcc gcggtgactc
     1561 atctccatca ccgacatgcc actgccatgc caatccagca gctcgtctcg cgcttgctcc
     1621 agcaccacct tgggaagcac agccggacca gcggaaaaat tgtagatctg cgtcaatcgg
     1681 ccaccaccta ctgttcgtct tgttcgtcgt cggtctcgac cactttttcc aggccggaga
     1741 gtttttcgcc ctcgcccaga ttgatcaggg tcaccccttg ggtcgctcgg cccagttcac
     1801 ggatctcctt gacgcgcgtg cggatcagca cgccgccggt cgtgattagc atgatctcgt
     1861 cgtcgtcgcc gaccaggcgc gctgcgacga ctgtgccgtt acgttcactg gtcacgatag
     1921 ccttcacgcc ctgcgtgccg cgaccggagt gacggaaatc agccagtgcc gtgcgtttgc
     1981 cataaccgtt ctcggtggcg accagcacgg tctgttgttc gttgtcggcg atcagcaagg
     2041 agatcacctg ctgtttggcc tgcaacttca tgccacgcac gccgcgcgtg ttgcggccca
     2101 tcgggcgtac gtcttcctca tcgaaccaga ccgccttgcc ggcattggac acgagaacga
     2161 catcgttggc accgttggtg atctcggctc cgatcaggaa atcgttgtca tcaaggttga
     2221 tggcgatgat gcctgacttg cgcgggttgg agaactcgac cagcgaggtc ttcttcacgg
     2281 tgcccatgct ggtcgccatg aagatgtaat ggttctcgtc gaactccttg accggcagga
     2341 tggcattgat cttctcgccc tcttccagcg ggaacaggtt gacgatcggc ttgccgcgac
     2401 tgatccggct tccctgtggc acctcataga ccttgagcca atagacgcgg ccgcggcttg
     2461 agaagcacag gatgtaatca tgtgtgttgg cgacgaacat cttgtcgatg aaatcgtctt
     2521 ccttggtcgc ggtcgcctgc ttgcctcggc cgccacgacg ctgagcacga tattcatcca
     2581 gtggctgcga tttgatgtag cctgtgtgcg atagcgtcac caccacgtct tgcggcgtga
     2641 tcaggtcttc cagcgacagg tcctgcgcat tctggatgat ctcgctacgg cgtttgtcgc
     2701 caaattgctg gcggatcgcc gagagttctt caacgatgat cgcggtcacg cgctcggact
     2761 tggccaggat atcgagcagg tcggcgatga catccatcac ctccttgtat tcgttgacga
     2821 tcttgtcttg ttccagaccg gtcaggcgtt gcagtcgcat ctgcaggatc tcttgcgcct
     2881 gcacgtcgga gagtcggtag cctttaggcg tcataccaaa gtcgactgac aacccgtcag
     2941 ggcgggaagc ttccatcgcg gcgcgtttca gcatctcttc aacgacaggt gatgtccagg
     3001 tacgcgccat caactcgcgc ttggcatccg gcggcgtcgg cgcagccttg atcagcgcga
     3061 tcatctcgtc gacattcgcc aatgccacgg ccagaccttc cagcacatgg ccgcgctcgc
     3121 gtgccttgcg cagttcgaat acggtgcggc gcgtgatgac ttcgcgacga tgacgcagga
     3181 aggcttccag catctgcttg aggttgagca gacgcggctg accgtcgagc agggccacca
     3241 tgttcatgcc gaaggtatcc tgcaactggg tctgcttgta cagattgttg aggatgactt
     3301 cgggtacttc gccgcgcttg agctcgatga ccacacgcat acccgacttg tcggattcat
     3361 cgcgcaggtc agagatgccc tcgatcttct tgtcgttgac cagttcggcg atcttttcga
     3421 tcagggactt cttgttcacc tgatacggca gctcatcgat gatgagcgcc tggcggttac
     3481 ccttctccat gtcttcgaca tgcgtgcggc cgcgcatgac aacacggcca cggccggtgc
     3541 ggtacccttc cttgacgccg accgttccat agatgatgcc cgcggtcggg aaatccggtg
     3601 ccgggatgat gtcgatcagt tcatcgacgg tggtctccgg atacttgagc agggtgagac
     3661 acgcgtcgat cacttcgcca aggttatgcg gcggaatgtt ggttgccata ccgacggcga
     3721 tcccggagct gccgttgatc agcaggttgg ggatacgtgc cggcatgatc agcggctcat
     3781 tctccgaacc gtcgtagttc gggccgaaat ccacggtctc tttgtcgatg tcagccagca
     3841 actcgtgcgc gatgcgcgcc atgcggattt cggtgtaacg catcgccgcc gcgttgtcgc
     3901 catccaccga accgaagttg ccctgaccgt cgaccagcat gtagcgcaac gagaaatcct
     3961 gcgccatgcg cacgatggtg tcgtacaccg cggtgtcgcc atgcggatga tacttaccga
     4021 tgacgtcacc aacgatacgc gccgatttct tgtaggcccg gttccagtcg ttgctcagct
     4081 cgtgcatggc gaaaagcacg cgccgatgca caggtttgag gccatcacgc acgtccggca
     4141 gtgcacgtcc cacgatcacg ctcatggcgt aatcgaggta gctgcggcgc atctcgtctt
     4201 ccagtttgac ggggatgatt tccttggcga ga
//
LOCUS       DATMTW010000129         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_615187, whole genome shotgun sequence.
ACCESSION   DATMTW010000129 DATMTW010000000
VERSION     DATMTW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615187"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VN084_02395"
     CDS             <1..757
                     /locus_tag="VN084_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWT28334.1"
                     /translation="ADIQRLAVPDMASLQYVFDAVAQIRKSIAGRVPLIGFAGSPWTL
                     ATYMVEGKGGTDFLNIKKMAYARPDLLHRILDITAQAVTQYLNAQIAAGAQAVMVFDS
                     WGGALSHNAYREFSLAYIEKIMNGLTRENEGRIVPRIAFTKGGGLWLEAQAGIGADAL
                     GLDWAVDIGEARKRVGDQVALQGNLDPAILLSTPEAIEKEVARILASYASAGGHGHGH
                     VFNLGHGITQWTPPENAAAMLQAVREHSTQYHR"
     gene            773..1258
                     /locus_tag="VN084_02400"
     CDS             773..1258
                     /locus_tag="VN084_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWT28335.1"
                     /translation="MPSEQASRTLYTSGYQGLVIDQFIARLKQAGIETVVDIRATPYS
                     RKPDFTKQALADHLEIAGIAYRHIPALGCPKPIRDAYGADHAQYEIHFKRYLATQQDA
                     VDALAVFSQTTSACLLCFEADHRECHRSLVAEACQRVQAMPVVHLGQVELPAQGLLGF
                     D"
     gene            1346..>2995
                     /locus_tag="VN084_02405"
     CDS             1346..>2995
                     /locus_tag="VN084_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWT28336.1"
                     /translation="MNLSSLNPRLRRFLVSRPLKIVLAILLVYLLLGYFAVNPLAQHL
                     LPWVAENKLASRITVDSVRFDPLRLILRADQLKLQTPDGKLLAGFDHLRVDLDSAGIF
                     RFAWRLSDIHLSGPQVVLDIAPDGKLNWAQLIAKLNEDKTEDDTGMSRLLIDHILIEN
                     GHIQYSERDRPTPFRMALKPLGVELEGLSTLPEDRGDYLIAAKLPEQGGTLKWKGDIA
                     LNPIASRGTLELQDINLAKLLQVVDPASMPVKVTSGGLQTRFAYDFSMVGDKAGPNPT
                     EPTPKATLKDILIRFTSLTADVEPHKALTVEDVTLKVPTLDLAMNKGTQLAFSDLDFT
                     ARQLVFRDAGFLQPLRAEIADLGLGFGFEMLGDTKAIHAARLNLGDMRLYSGEGAQPV
                     ASVAKVAARDGEVDLGKSAVALASLVVSGLTADVIRNADGKLNWQQILEPKATDHAAS
                     QVAAAGGADSASPSSPSSSAPPSSGASAASSASPWTLALAELALEDGGIRFVDNTLRE
                     PVKFDIQQVSLSMAQASQDLSKPLPVKASFKVKQGGQFSADG"
BASE COUNT          579 a          960 c          895 g          561 t
ORIGIN      
        1 agccgatatc cagagactcg ccgtgcccga catggcgagc ctgcaatacg tattcgacgc
       61 cgtagcgcag atccgcaaga gcatcgctgg ccgcgtgccg ctgatcggtt tcgccggcag
      121 cccttggacg ctggcgacct acatggtcga aggtaagggc ggcaccgatt tcctcaacat
      181 caagaagatg gcttacgcac ggcctgacct gttgcaccgt atcctcgata tcacggcgca
      241 ggcggtcacg cagtacctca acgcccagat cgccgccggc gcgcaggccg tgatggtgtt
      301 cgattcctgg ggcggcgcgc tgtcgcataa cgcttatcgc gagttttccc tggcctatat
      361 cgaaaaaatc atgaatggcc tgactcgcga gaacgaaggc cgcatcgtgc cgcgtatcgc
      421 attcaccaag ggcggtggct tgtggctgga ggcgcaggcc ggcatcggcg cagacgcgtt
      481 aggcctggac tgggcggtgg acattggcga ggcacgcaag cgcgtcggtg accaggtcgc
      541 gctgcaaggc aatctcgatc cggcgatcct gctctccacg ccagaagcca tcgaaaagga
      601 agtcgccaga atcctggcaa gctatgcttc tgctgggggc cacggtcacg gccacgtgtt
      661 caacctgggg catggcatca cccagtggac gccgcctgaa aacgccgctg ctatgctgca
      721 agcggttcgc gagcacagca cgcaatacca tcggtaggac cgcaggactg gcatgccttc
      781 cgaacaggca tctcgcaccc tctatacctc gggctatcag ggcttggtca tcgaccagtt
      841 catcgcccgg ctgaaacaag ccggcatcga aaccgtggtc gatatccgtg ccacgccgta
      901 ttcgcgcaag ccggatttca ccaagcaggc acttgccgac catcttgaga tagccggtat
      961 cgcttatcgc cacatccccg cattaggttg cccgaagccg atccgcgatg cctatggcgc
     1021 agatcatgcg cagtacgaga tccacttcaa gcgctacctg gcgacccagc aggatgccgt
     1081 cgacgcgctg gctgtgttca gccagaccac atccgcctgc ctgttgtgct tcgaggccga
     1141 ccaccgcgaa tgtcatcgca gcctcgtcgc cgaggcctgc cagcgtgtgc aggccatgcc
     1201 tgtcgtgcat ctcgggcaag tcgagcttcc cgcgcagggt ctgcttggct ttgactgaac
     1261 aagcgctcga ctgaacaggc cgtgcgggcg agtgctgcgc tatacttgag tcatccatcc
     1321 tcaagcatga tccggcttcg caaccatgaa cctgtccagc cttaacccgc gcctccgtcg
     1381 cttcctggtc tcccggccgc tcaagatcgt actcgccatc ctgctggtct atctgttgct
     1441 cggctatttt gccgtcaacc cgctcgccca gcacctactg ccatgggtgg ccgagaacaa
     1501 gctggcgagc cgcatcactg tggatagcgt gagattcgat cccctgcgcc tgatcctgcg
     1561 cgccgaccaa ctgaaactgc aaacgccgga tggcaagctg ttagccggtt tcgatcatct
     1621 gcgggtcgat ctcgatagcg ccggcatctt ccgtttcgcc tggcggctca gcgatatcca
     1681 tctgagcggc ccgcaggtcg tgctggacat cgccccggac ggtaagctga actgggcgca
     1741 gcttatcgcg aagctcaacg aggacaagac cgaagacgat actggcatgt cgcgcttgct
     1801 gatcgaccat atcctgatcg agaacggcca tatccagtac agcgagcgtg accggccaac
     1861 gcctttccgc atggcgctga agccgctggg tgtcgagttg gaaggcttgt ctaccctgcc
     1921 cgaggatcgc ggtgactacc tgatcgcggc caagctgccg gagcagggcg gcaccctcaa
     1981 atggaaaggc gacatcgcgc tcaaccccat cgcatcgaga ggcacgctcg agctgcaaga
     2041 catcaacctc gccaagttgt tgcaggtggt cgatcccgcg tccatgccgg tcaaggtgac
     2101 ctccggcggc ctgcagacgc gcttcgccta tgacttttcg atggtcgggg ataaagcggg
     2161 gccaaatccg acagagccca cgcctaaagc cacgctgaag gacattctca tccggttcac
     2221 cagcctaacg gccgatgtcg agccgcataa agcgctgacg gtagaggacg tgacgctgaa
     2281 ggtgccgacg ctggatctgg cgatgaacaa gggtacgcag ctggcgttca gcgaccttga
     2341 tttcaccgcc cggcaactgg tattccgcga tgccggattc ctgcagccgc tgcgtgcaga
     2401 gatcgcggac ctcggtctcg gcttcggttt cgagatgctg ggggatacga aagcgatcca
     2461 tgccgccagg ctcaaccttg gcgacatgcg cctctattcc ggcgagggtg cccaaccggt
     2521 agccagcgtg gccaaggtcg ctgcgcgtga tggtgaggtc gatctcggca agagtgctgt
     2581 ggcgctggcg tcgctggtag tgtcgggcct gacggcagac gtgatccgca atgccgacgg
     2641 caagctgaac tggcagcaga tcctcgagcc gaaggctacc gaccatgctg ccagccaggt
     2701 tgcggccgct ggcggagcag actccgcttc tccctcgtct ccatcatcca gcgcacctcc
     2761 atctagcggt gcttcagccg cttccagtgc ctctccgtgg acactggcac tggcagagtt
     2821 ggccctggaa gatggcggta tccgtttcgt cgacaacacc ctacgcgaac cggtcaagtt
     2881 cgatatccag caggtctccc tgtcgatggc gcaggcctcg caggacctga gcaaacccct
     2941 gccggtcaag gccagtttca aggtcaagca gggcggccag ttcagcgcag acggc
//
LOCUS       DATMTW010000130         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_413423, whole genome shotgun sequence.
ACCESSION   DATMTW010000130 DATMTW010000000
VERSION     DATMTW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413423"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="VN084_02410"
     CDS             <1..1753
                     /locus_tag="VN084_02410"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWT28337.1"
                     /translation="AIAGMAVEHADLAGPAAQGPADQGLALGQAGVADREAGGEVVRA
                     VQHQVIPRHHRPGVGLTVQDILRRFLAQHSDWKLLPEKIAIQLNDTHPAVAVAEMMYQ
                     LVDVHRLKWDQAWKMVNRIFAYTNHTLMPEALETWPVELFGRLLPRHLEIIYQINHEF
                     LHMVNHRFPGDTDLLSRVSIIDESHGRRVRMAHLAVVGSNTVNGVAALHSELLKTTLF
                     ADFNRIYPGKFINVTNGITPRRWLNQCNPGLTHLIASVIGDGFQKDLSKLVKLTPLAD
                     DADFRKAFHSVKQHNKQRLANRIEQLTGVKLNTDSLFDVQIKRIHEYKRQLLNLLHVI
                     TLYNRIRKDGGKDFTPRTVIFGGKAAPGYWMAKQIIRLINDVAAIVNDDPAVGDKLKV
                     VFYPNYEVSAAELLFPGSDLSEQISTAGTEASGTGNMKMALNGALTIGTLDGANVEIK
                     EEVGEDNIFIFGLTTQEVAETKAAGYNPWDYYHANAELREVLDMIAGGFFSVDEPGRY
                     QAIFDTLLHKGDHYMLLADYASYIETQDKVSALYRNREEWTRRAVLNVARMSKFSSDR
                     TIGEYVRNIWKVTPVKR"
     gene            1917..>2228
                     /locus_tag="VN084_02415"
     CDS             1917..>2228
                     /locus_tag="VN084_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HWT28338.1"
                     /translation="MRRVVTALVVLFFSLQASAVSLGDLTNTEASSGLKEALIQGAGK
                     AVGKLGAVDGFLGNPKVKIPLPDSLKKAEKAMRMFGMGKQADELVLKINRASEAAVPE
                     AK"
BASE COUNT          469 a          710 c          641 g          408 t
ORIGIN      
        1 agcgatagca gggatggccg tggaacacgc cgacctggcc ggtccagcgg cccagggccc
       61 cgctgaccag ggtcttgccc tcggccaggc aggcgtcgct gaccgcgaag cgggtggcga
      121 agtcgtccgt gccgtccagc accaggtcat accgcgacac caccgcccgg gcgttggcct
      181 caccgtccag gatatcctgc gccgcttcct cgcccagcat agcgactgga aactcttgcc
      241 ggagaagatc gccatccagc tcaatgacac gcacccggcg gtcgcggtcg cggagatgat
      301 gtaccagctg gtcgacgtgc atcgcctgaa atgggatcag gcctggaaga tggtcaaccg
      361 catcttcgcc tacaccaatc acacgctcat gccggaagcc ctggagacct ggcctgtcga
      421 gctgttcggt cgcctgttgc cgcggcatct ggagatcatc taccagatca accacgaatt
      481 cctgcacatg gtgaaccaca ggttccccgg cgataccgac ctcctgagtc gcgtctccat
      541 catcgacgag agtcatggcc gccgtgtgcg catggctcac ctggccgtcg tcggcagcaa
      601 taccgtgaac ggcgtagctg cgctgcatag cgaactgctc aagaccacgc tgttcgccga
      661 tttcaaccgc atctatccgg gcaagttcat caatgtcacc aatggcatca ccccgcggcg
      721 ctggctgaac cagtgcaatc cggggctgac acacctgatc gcttcggtga tcggcgacgg
      781 cttccagaaa gacctttcca agctggtcaa gctcacgcca ctcgcggatg acgcggattt
      841 ccgcaaggct ttccatagcg tcaaacagca taacaaacag cgcctggcga accgcatcga
      901 gcagctgacc ggcgtgaaac tcaataccga cagcctgttc gacgtgcaga tcaagcgtat
      961 ccacgaatac aagcgccaac tgctcaacct gttgcatgtc atcacgctct ataaccgtat
     1021 ccgcaaagat ggcggcaagg acttcacgcc gcgtacggtg atcttcggcg gcaaggcagc
     1081 cccgggctac tggatggcga aacagatcat ccgcctcatc aacgacgtcg ccgccatcgt
     1141 caatgacgat cctgccgtgg gcgacaagct caaggtggtg ttctatccca attatgaagt
     1201 ctccgctgcc gagctgctgt tccccggcag cgacctctcc gagcagatat ccacggctgg
     1261 caccgaggca tcaggtaccg gaaacatgaa gatggcgctc aatggcgcgc tcactatcgg
     1321 cacgctcgat ggcgccaatg tcgagatcaa ggaagaggtc ggcgaggaca atatcttcat
     1381 cttcggcctc acgacgcagg aagtcgccga gaccaaggcc gccggctaca acccatggga
     1441 ctactatcat gccaatgccg agctgcgtga agtgctcgat atgatcgccg ggggcttctt
     1501 ctcggtggac gagccggggc gctaccaggc gatcttcgat accttgctgc acaagggcga
     1561 ccactacatg ctgctggccg attacgcgag ctatatcgag actcaggaca aggtcagtgc
     1621 gctctatcgt aaccgcgagg aatggacgcg ccgcgccgtc ctcaacgtgg cgcgcatgag
     1681 caagttctcc agtgaccgta ccatcggcga atacgtacgc aatatctgga aggtcacacc
     1741 ggtcaagcgc tgaccgccgg cgtctggccc atcgtggccg gctggtgatc tcggcgctgc
     1801 gcgcgctgtc ttagaactgt gcgcgctgcc ttagaactgt gcgggccgcc ttggaactgt
     1861 gcgggcaagc agagtagact aggcttgctt gcccaatctt tcatctggag gtcttcatgc
     1921 gtcgcgtcgt cacagccctg gtagtcctat tcttttcgct gcaggccagt gcagtcagcc
     1981 tcggcgatct caccaacacc gaagccagca gcggcctcaa ggaggcattg atccagggtg
     2041 ccggcaaggc ggtcggtaaa ctgggcgccg tcgatggctt cctcggcaat cccaaggtca
     2101 agatcccctt gccggattca ctgaaaaagg ccgaaaaagc catgcgcatg ttcggcatgg
     2161 gtaaacaggc cgatgagctc gtgctcaaga tcaaccgcgc ctccgaggcc gctgtgcctg
     2221 aggccaag
//
LOCUS       DATMTW010000131         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_548204, whole genome shotgun sequence.
ACCESSION   DATMTW010000131 DATMTW010000000
VERSION     DATMTW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548204"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="VN084_02420"
     CDS             complement(<1..2504)
                     /locus_tag="VN084_02420"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWT28339.1"
                     /translation="MELAKSFEPADIERRWYPAWESAGHFKAGLDTSNPNAFCILLPP
                     PNVTGTLHMGHGFNQTIMDALTRFHRMRGDNTLWQPGTDHAGIATQIVVERQLDAQGV
                     SRHDLGREKFLEKVWEWKEFSGNTITKQMRRLGTSPDWSRERFTMDSGLSKIVTETFV
                     RLYNEGLIYRGKRLVNWDPVLHTAVSDLEVVSEEEDGSLWHIRYPLADGSGSLTVATT
                     RPETMLGDTAVMVHPEDERYAHLIGKTVKLPLTNGTDALDARGWREIPIIADEYVDRE
                     FGTGVVKVTPAHDFNDYAVGQRHKLPMICILTLDAKINDEAPVQYRGLDRFAARKQIV
                     ADLEAAGLLVQIDKHKLKVPRGDRTNVVIEPMLTDQWFVAMTKPGANGKSITEQALEV
                     VASGEIKFHPENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYGDNGKVYVAHDQSEA
                     EAMAAKDGYSGNLKRDDDVLDTWYSSALWPFSTLDWTPEWPAKSNPALDLYLPSTVLV
                     TGFDIIFFWVARMVMMTKHITGRIPFKDVYVHGLIRDAEGQKMSKSKGNVLDPIDLID
                     GIDLDALVAKRTTGLMNPKQAEQIAKRTKKEFPEGIPGFGTDALRFTFLSLASPGRDI
                     KFDMQRCEGYRNFCNKLWNATRFVLMNCEGQDCGFAKPGPCAPGDCAPDGYHHFSQAD
                     RWIVSELQRVEAEAAQHFADYRFDLLAKSVYEFVWDQYCDWYLELAKVQIQTGNEATQ
                     RGTRRTLLRVLETILRLAHPLIPFITEELWQTVAPLTGIDVAGKSIMLQAYPKSQPEK
                     IDTAAEAWMAQLKEMVTACRSLRGEMNISPAQRVPLV"
     gene            complement(2590..>2775)
                     /locus_tag="VN084_02425"
     CDS             complement(2590..>2775)
                     /locus_tag="VN084_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28340.1"
                     /translation="ELARALERWIATQLPDALAQALDSVAEQLKLELAARLRAELLTR
                     PMFDQQPLFQPARKPGP"
BASE COUNT          487 a          816 c          916 g          556 t
ORIGIN      
        1 accagcggca cgcgctgggc cggcgagatg ttcatttcgc cacgcaggct gcggcaggcc
       61 gtaaccattt ccttgagttg cgccatccag gcttcggcgg ccgtatcgat cttctccggc
      121 tgcgacttgg ggtaggcctg cagcatgatg gacttgccgg ccacatcgat gccggtcagc
      181 ggcgcgacgg tctgccacag ctcttcggta atgaagggaa tcagcggatg agcaaggcgc
      241 agaatggttt cgagcacgcg cagcagggtg cggcgggtgc cgcgctgcgt cgcttcgttt
      301 ccggtctgga tctgtacctt ggccagctcc agataccagt cgcagtactg atcccagacg
      361 aattcataca cgctcttggc gagcaggtcg aaacggtagt cggcgaaatg ttgcgccgct
      421 tcggcttcga cgcgctgcag ttcggagacg atccagcgat cggcctgcga gaagtggtgg
      481 tagccatccg gcgcacaatc gccgggagca caggggccag gttttgcaaa cccacaatct
      541 tgcccttcgc agttcatcag cacgaagcgc gttgcgttcc acagcttgtt gcagaagttg
      601 cgataaccct cgcaacgctg catgtcgaac ttgatgtcgc ggccgggcga ggcgagcgag
      661 aggaaggtga agcgcagcgc atcggtgccg aagccgggaa tcccttccgg gaactccttc
      721 ttggtgcgct tggcgatctg ctcggcctgc ttcggattca tgagaccggt ggtgcgcttg
      781 gcgaccaggg cgtccagatc gatgccatcg atcaggtcga tcgggtcgag cacattgccc
      841 ttggacttcg acatcttctg gccttcggca tcgcggatca ggccgtgcac atacacgtcc
      901 ttaaacggga tcctgccggt gatgtgcttg gtcatcatga ccatgcgcgc cacccagaag
      961 aagatgatgt caaagcccgt gaccagcacg gtcgagggca ggtagaggtc gagcgcggga
     1021 ttcgacttgg ccggccactc cggcgtccag tccagcgtcg agaacggcca cagcgccgac
     1081 gagtaccagg tatcgagtac atcgtcgtcg cgcttcaggt tgccgctata gccatccttg
     1141 gctgccatgg cttcggcttc gctttggtca tgtgcgacgt agaccttgcc gttgtcgccg
     1201 taccaggccg ggatctgatg gccccaccac agctggcgtg agatgcacca gtcctggatg
     1261 ttgttcagcc actggttgta ggtattgacc cagttttccg ggtggaactt gatctcgcct
     1321 gaggcgacaa cctcaagggc ttgttctgtg atgctcttgc cgttggcgcc tggcttggtc
     1381 atggcgacga accactggtc ggtgagcatg ggctcgatca cgacattggt acggtcgccg
     1441 cgcggcacct tcagcttgtg cttgtcgatc tgcaccagca ggccggcggc ttcgagatcg
     1501 gcgacgatct gtttgcgggc tgcaaagcga tccaggccgc ggtactggac cggggcttcg
     1561 tcgttgatct tggcatccag cgtgaggatg cagatcatcg gcagcttgtg acgttggccg
     1621 acggcatagt cgttgaagtc gtgcgccggg gtgaccttga ccacgccggt accgaactcg
     1681 cgatcgacgt attcgtcggc gatgatcgga atctcgcgcc aaccgcgcgc atcaagcgcg
     1741 tcggttccat tggtcagcgg cagcttcacg gtcttgccga tcaggtgcgc ataacgctca
     1801 tcttccggat gcaccatcac ggcggtgtcg ccgagcatgg tttctggacg cgtggtggct
     1861 acggtcagcg aacccgagcc gtcggcgagc gggtagcgga tgtgccacag cgaaccatcc
     1921 tcttcctcag atacgacttc gaggtcgctg accgccgtgt gcagcaccgg gtcccaattg
     1981 acgaggcgct tgccgcggta gatcaggcct tcgttgtaaa ggcgcacgaa ggtttcggta
     2041 acgatcttgg agagaccgct gtccatggta aagcgctcgc gcgaccagtc gggcgaggtg
     2101 ccgaggcgac gcatctgctt ggtgatggtg ttgccggaga actccttcca ctcccacacc
     2161 ttttccagga acttctcgcg cccgagatca tgacgcgaga cgccctgcgc atcgagctgg
     2221 cgctcaacca cgatctgggt ggcgatgccg gcatggtcgg tgcccggctg ccacagcgtg
     2281 ttgtcgccgc gcatccgatg aaagcgcgtc agcgcatcca tgatggtctg gttgaaaccg
     2341 tggcccatgt gcagcgtgcc cgtgacattg ggcggcggca gcaggatgca gaaggcgttg
     2401 ggattcgagg tgtcgagtcc ggccttgaaa tgaccggcgg attcccaggc cggataccag
     2461 cggcgctcga tatcggcggg ctcgaagctc ttggcgagtt ccatgatgtg cagcggcaaa
     2521 gacgaaaccc gcaattatac gggagtggct gcagagaacc gccttggcga aggctggtcc
     2581 ggggcgcggc taggggccgg gtttacgggc gggctggaat agcggctgct ggtcgaacat
     2641 ggggcgggtc agcagctcgg cacgcaggcg cgcggcaagc tcgagcttca actgctcggc
     2701 gacgctgtcg agcgcttgcg ccagagcatc cggcagctgc gtggcgatcc agcgttccag
     2761 cgcgcgcgcc agttc
//
LOCUS       DATMTW010000132         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_313149, whole genome shotgun sequence.
ACCESSION   DATMTW010000132 DATMTW010000000
VERSION     DATMTW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313149"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..543)
                     /locus_tag="VN084_02430"
     CDS             complement(52..543)
                     /locus_tag="VN084_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWT28341.1"
                     /translation="MASVNKVILVGNLGRDPEMRYMPSGDALASFSIATTDTWKDKNG
                     QKQERTEWHRISMFGKQAEVAGEYLKKGSSVYIEGRLQTRKWQDKEGNERQTTEVVAD
                     RMQMLGGRSGGGNTYEVMDDDMNQDAPAPRQSAPASAPKQSPAQKPAGGGGFDDFEDD
                     IPF"
     gene            complement(560..1936)
                     /locus_tag="VN084_02435"
     CDS             complement(560..1936)
                     /locus_tag="VN084_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWT28342.1"
                     /translation="MAKTEQMSALELRASMSLASIYGLRMLGMFLILPIFAIYAETLP
                     GDKNHVLIGLALGAYGLTQAIFQLPFGMASDRYGRKRMIYIGLVLFVIGSLVAGFAHD
                     IYTVIIGRAIQGAGAVSAVVTALVADLTREEHRTKAMAMIGGTIGITFALSLILGPAL
                     NQWIGVPGIFLLTGALAALAILDVKFLVPDPLITRFHSDAEAAPGKLKEVLKDFQLLR
                     LNFGIFALHAAQMAMFVVVPFAIKQTSGMNENDHWMIYLPVMVVSFALMVPAIIYGEK
                     RARLKQVFVGAIAIMLGAQLLFASSISHFWGIIISLSAYFIAFNVLEASLPSLISKVA
                     PAASKGTAIGVYNTAQSLGVFVGGTLGGYLSHYHGYASVFIFCSALMGLWLIFAASMK
                     TPPAVKSKMYHVESLSEEAAANLSRQLGTLTGVREAVVLPDEGLVILKVDMQQAFDES
                     EAMRLIAG"
     gene            2005..>2963
                     /locus_tag="VN084_02440"
     CDS             2005..>2963
                     /locus_tag="VN084_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWT28343.1"
                     /translation="MDYIRIRGARTHNLKNVSLDLPRNKMIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLARMDKPDVDLIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLYARAGDPECPDHGIKLEAQTVSQMVDHVLALPEDTKLMILAPVVSNRKG
                     EQLDLLEELKAQGFVRVRVDGEIYEMDAVPKLAKTVKHNVDVVVDRLKVRADMKQRIA
                     ESFETALRLAEGKAVAMEMDTGKEYLFSAKFSCPVCDYSLAELEPRLFSFNNPMGACP
                     QCDGLGQISFFDPKRVVAFPHLSLAAGAIKGWDKRNQFYFQMLT"
BASE COUNT          604 a          896 c          885 g          578 t
ORIGIN      
        1 agctgacgca agtcagctgg cttcttcgtt tgcaaccgcg gggacagacc gctagaacgg
       61 aatatcgtcc tcgaaatcgt caaaaccgcc accgcctgcc ggcttttgcg ccggtgactg
      121 cttgggtgcg ctggcgggtg ccgattggcg cggcgccggg gcgtcctgat tcatgtcgtc
      181 gtccatgact tcataggtgt tgccgccgcc gctgcggcca ccgagcatct gcatgcggtc
      241 ggcgacgacc tcggtggtct ggcgttcatt gccttcctta tcctgccatt tgcgtgtctg
      301 caggcggcct tcgatataaa cggaagaccc tttcttcagg tactcgccag cgacttcggc
      361 ttgtttgccg aacatggaga tacggtgcca ttcggtacgt tcctgctttt gtccgttctt
      421 gtctttccag gtatccgtgg tggcgatact gaacgaggcc agcgcgtcgc cggacggcat
      481 gtagcgcatc tccggatcac gccccagatt accgaccaga atcactttgt ttaccgatgc
      541 catctttaat ccccttgtgc tatcccgcta ttaatcgcat ggcttccgac tcgtcgaaag
      601 cctgctgcat gtcgacctta agtataacca aaccttcatc cggcagcact accgcttcgc
      661 gcacgccagt cagcgtgccg agttggcgcg acaggttggc ggcggcttcc tcgctcaggc
      721 tctcgacgtg atacatcttg ctcttcaccg ctggcggcgt tttcatgctg gcggcgaaga
      781 tcagccataa ccccatgagt gcgctgcaga agatgaatac cgaagcgtag ccgtggtaat
      841 gcgacaggta accgcccagt gtgccgccga cgaatacgcc gagagactgc gccgtgttgt
      901 agacgccgat ggcggtgccc ttggaggcag ccggggcgac cttggagatc agcgaaggca
      961 aactcgcttc aaggacgttg aaagcgatga aataggccga cagcgaaatg atgatgcccc
     1021 agaaatggct gatggaagac gcgaacagca gttgcgcccc cagcatgatg gcgatcgcgc
     1081 cgacaaacac ctgcttgagg cgcgcacgct tctcgccgta gatgatggcc ggcaccatga
     1141 gcgcgaagga caccaccatc accggcaggt agatcatcca gtggtcgttc tcgttcatgc
     1201 cgctggtctg cttgatggcg aacggcacga cgacgaacat ggccatctgc gccgcatgca
     1261 aggcaaagat accgaaattg agccgcagca gctggaaatc cttgagcact tccttgagct
     1321 tgccgggggc cgcctctgca tccgagtgga agcgcgtgat aaggggatcc ggcaccagga
     1381 atttcacgtc gaggatcgcc agcgcggcca gcgccccggt caacaggaag ataccgggca
     1441 cgccgatcca ctggttgagc gccggcccga ggatcaaaga caacgcaaag gtgatcccga
     1501 tggtgccgcc gatcatggcc atggccttgg ttcggtgctc ctcgcgcgtc aggtcggcga
     1561 ccagcgccgt cactaccgca gacacggcac cggcgccctg gatggcacgg ccgatgatga
     1621 cggtatagat gtcatgggcg aagccggcga ccaggctgcc gatgacgaac agcacgaggc
     1681 cgatgtagat catgcgcttg cggccgtagc ggtcggaggc catgccgaac ggcaactgga
     1741 agatcgcctg cgtcaggcca taggcgccaa gcgcaaggcc gatcagcacg tgatttttgt
     1801 cgcctggcag ggtttcggcg tagatcgcga agatcggcag gatgaggaac atgcctagca
     1861 tgcgcaggcc gtagatcgag gccagactca tgcttgcccg gagttcaagc gcggacatct
     1921 gttcggtttt tgccatggaa agtttgaatg aaaggctgaa aattgcgtat attaacaggt
     1981 tgccccactc gtcgtcagag ccacatggat tacatacgta tacgcggtgc ccgcacccac
     2041 aatctgaaga acgtctcgct cgatctgcca cgcaacaaga tgatcgtcat caccggcctc
     2101 tccgggtcag gtaagtcctc actcgcgttc gataccttgt acgccgaggg tcagcgacgc
     2161 tacgtcgaat cgctgtcggc ctacgcccgg cagttcctcg cacgcatgga caagccggat
     2221 gtcgatctga tcgaaggcct gtcgccggcc atctccatcg agcagaagtc gacttcgcac
     2281 aacccgcgtt cgacggtcgg cactgtcacc gagatccacg attacctgcg tctgctctat
     2341 gcgcgcgccg gcgatcctga gtgcccggac catgggatca agctcgaggc gcagaccgtg
     2401 tcgcagatgg tggaccatgt gctggcgcta ccggaagaca ccaagctcat gatcctggcg
     2461 ccggtcgtca gcaaccgcaa gggcgagcaa ctagatctgc tggaagaact caaggcccag
     2521 ggtttcgttc gcgtgcgcgt cgacggcgag atctacgaga tggacgccgt gcccaagctc
     2581 gccaagaccg tgaagcacaa tgtggatgtc gtagtggatc ggctgaaagt acgcgcagac
     2641 atgaagcagc gtatcgccga atccttcgag accgccctgc gtctggctga aggcaaggcc
     2701 gtggcgatgg agatggacac cggcaaggaa tacctgttct cggccaagtt ctcctgcccg
     2761 gtttgtgact actcactggc agaactcgag ccgcgcctgt tctcgttcaa caacccgatg
     2821 ggcgcctgcc cgcaatgcga tggcctggga cagatcagct tcttcgaccc caagcgcgtc
     2881 gtcgccttcc cgcatctgtc gctggccgcc ggcgccatca agggctggga caagcgcaac
     2941 cagttctatt tccagatgct gac
//
LOCUS       DATMTW010000133         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_615721, whole genome shotgun sequence.
ACCESSION   DATMTW010000133 DATMTW010000000
VERSION     DATMTW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615721"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VN084_02445"
     CDS             <1..193
                     /locus_tag="VN084_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWT28344.1"
                     /translation="FVACLMALQAQALPPTAHLKQSDPECDLDYVPNVGRQNVRVDTV
                     MSNSFAFGGTSAVLIARKP"
     gene            complement(216..1370)
                     /gene="clsB"
                     /locus_tag="VN084_02450"
     CDS             complement(216..1370)
                     /gene="clsB"
                     /locus_tag="VN084_02450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HWT28345.1"
                     /translation="MNHYFSGNQITLLRNGTEYFPAIEAAIDAARLEIHLQAYIYEKD
                     ETGIRIGEALKRAVDRGVVVCVLLDGYGSKNLPSDYVETLRAAGIKLMFFRPKISPWT
                     LKRNRLRRLHRKVLVVDGCLAFVGGINIIDDQNCPGNNAPRVDYAVKVEGPLVRRIHT
                     SIYRHWRRISWMHFRQAHDNPVERLPQPTTAGNMRAAYVIRNNVLHRRDIERAYLRAI
                     GTARREILIANAYFLPGKAFRRALIAASKRGVRVRVLLQGKMEYWLMFATHAVYGLFL
                     RHRIEIYEYRKSFMHSKVAVVDDLWATVGSSNIDPMSLLLAHEANVVVRNREFATLLR
                     EDIEQAIEAGGYRIRTEDWNGRHVFRRFFSWATYLLVRIMMGIAGFPKGR"
     gene            complement(1370..2119)
                     /locus_tag="VN084_02455"
     CDS             complement(1370..2119)
                     /locus_tag="VN084_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWT28346.1"
                     /translation="MQNTLRIATFNIHKGVTSFNARFALHQQRDLIRKLHADIVFLQE
                     VRDLHIPHSRRFSGWPENGQAEFLADAVWPDYAYGRNSVYPAGHHGNALLSKFPIISS
                     VNEDISAHAVEQRGMLHCEIAVPGWQQPLHCLCVHLGLFSRWRRKQVHAMRERIEATI
                     PSDAPLIIAGDFNDWGMSAGKILVEGLHLREVFEHHHGRSARSFPSVLPMFRLDRIYT
                     RGFHVHHAEVHASADFAKVSDHAALSALLSR"
     gene            complement(2244..3341)
                     /gene="nadA"
                     /locus_tag="VN084_02460"
     CDS             complement(2244..3341)
                     /gene="nadA"
                     /locus_tag="VN084_02460"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWT28347.1"
                     /translation="MQTATIKFERFQNSADDECQQRIVAAKEKLGDRLVILGHHYQQE
                     GVYKHAHFTGDSLKLSRHAADVDAEFIVFLGVHFMAEVADILSRPEQIAILPDLSAGC
                     SMADMANLAKVERSFRELNKVLDFDKEVTPLTYINSAADLKAFCGEHGGIVCTSTNAT
                     KMLEWSFARREKVLFFPDQNLGRWSGYKMGIPLEQMPVWDPDQPMGGLTEEQIKNARI
                     LLWKGHCAVHQMFRPQNIIKFRNEHPEGKVISHPEAPFEVCLQSDYVGSTEYIVKTIT
                     DSAPGTRWLVGTELNLVNRLAERFKPDGKIVQFMSHVVCECSTMARIDPQHLAWTLEN
                     LAEGNVVNQIKVLPEEAKLAKLALDRMLEAS"
     gene            complement(3351..3803)
                     /gene="nudB"
                     /locus_tag="VN084_02465"
     CDS             complement(3351..3803)
                     /gene="nudB"
                     /locus_tag="VN084_02465"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HWT28348.1"
                     /translation="MVSYKTPVSALILIHTPDLKVLIMERADKPGFWQSVTGSIEADE
                     TPYDAAIREVREETGLDALDYKFEDWQASNTYEIYPHWRHRYAPGVTENVEHLFGLEL
                     PAPVDVQLAPDEHVRYEWVDWREAAKRVFSWTNVDALKRLGERHHLKL"
     gene            complement(3800..>4292)
                     /locus_tag="VN084_02470"
     CDS             complement(3800..>4292)
                     /locus_tag="VN084_02470"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWT28349.1"
                     /translation="YPKQGVQFRDITTLLKDPLGLKVTIDEFHKRYVDQRIDKVVGVE
                     SRGFLVGAPLAYKLGVGFVPIRKGGKLPAETMGHDYELEYGSDRVEIHKDAIEKGERI
                     LLVDDLIATGGTALASAALIEKLGGEVVECAFIIDLISLGGRKRLEAKGHTMFALCEF
                     EGE"
BASE COUNT          805 a         1295 c         1321 g          871 t
ORIGIN      
        1 gttcgtcgcc tgcctcatgg cactgcaagc gcaagccttg ccgccgaccg cacacctgaa
       61 acagtccgac ccggaatgcg acctggacta cgtgccgaac gtcggccgcc agaatgtcag
      121 ggtcgatacc gtcatgagca attccttcgc ttttggcggc accagcgccg tcctcatcgc
      181 gcgcaagccc tgaagcagcc agcgccgcgc ttgcgctaac gccctttcgg gaagccggcg
      241 atccccatca tgatgcgcac cagcaggtag gtagcccagg agaagaaacg ccggaacacg
      301 tgtcgcccgt tccagtcttc ggtgcggatg cgatagccgc cggcctcgat cgcctgttcg
      361 atatcttctc gcagtagcgt ggcgaactca cggttacgca ccaccacgtt ggcttcgtgc
      421 gcgagcaaca ggctcatggg gtcgatgttc gacgagccga cggtcgccca cagatcgtcg
      481 acgacagcga ccttgctgtg catgaagctc ttgcggtatt cgtagatctc gatccggtgt
      541 cgcaggaaca ggccatagac cgcgtgcgtg gcgaacatca gccagtattc catcttgccc
      601 tgcagcagca cacgcacacg caccccacgc ttgctggcgg cgatgagggc acgacggaag
      661 gccttgccgg gcaggaaata ggcattggcg atgaggatct cccgccgggc cgtgccgatc
      721 gcgcgcagat aagctctttc gatgtcgcgc cggtgcagta cgttgttgcg gatgacatac
      781 gccgcacgca tattgccggc ggtcgtcggt tgcggcagcc gttcgaccgg gttgtcgtgt
      841 gcctgacgga aatgcatcca gctgatacga cgccagtggc ggtagatgct ggtatggatg
      901 cggcgcacca gcggcccttc gaccttgacc gcataatcga cgcgtggcgc gttgttgccg
      961 ggacagttct ggtcatcgat gatattgatg ccgccgacga aagccaggca gccgtccacc
     1021 accaggacct tgcgatgcag gcggcgcagg cggttgcgtt tcagggtcca cggcgagatc
     1081 ttcggccgga agaacatgag cttgataccg gccgcgcgca acgtctcgac ataatctgaa
     1141 ggcaggttct tgctgccgta gccatcgagc agcacgcata ccaccacacc acgatcgacg
     1201 gcacgcttga gggcttcacc gatgcggatg ccggtctcgt ccttttcgta gatataggcc
     1261 tgcagatgga tctccagcct ggcggcgtcg atggccgcct cgatcgccgg gaaatactcc
     1321 gtgccgttac gtaacagcgt gatctggttg ccggagaagt aatgattcat cagcgtgata
     1381 atagcgcgct caacgcagcg tgatcggaga ccttggcgaa atcggcgctg gcatggactt
     1441 cggcatggtg cacatggaag ccgcgtgtgt agatgcggtc gagccggaac atgggcagca
     1501 cagaggggaa actgcgggcc gagcggccgt gatggtgctc gaacacttcg cgcaggtgca
     1561 ggccttcgac caggatcttg ccggcgctca tgccccagtc gttgaaatcg ccggcgatga
     1621 tgagcggcgc gtcggacggg atggtcgcct cgatgcgttc gcgcatggca tgcacctgct
     1681 tgcggcgcca gcgcgagaac agccccagat gcacgcagag gcagtgcaac ggctgttgcc
     1741 agcccggtac cgcaatctcg cagtgcagca tcccacgctg ctcgacagca tgcgccgaga
     1801 tatcttcatt gacgctactg atgatgggga atttcgacag cagggcattg ccgtgatggc
     1861 cggccggata caccgaattc cgcccatagg catagtccgg ccataccgcg tcggcgagaa
     1921 actcggcctg gccgttctcc ggccagccgg agaagcgacg actgtgtggg atatgcaggt
     1981 cacgcacttc ctgcaggaaa acgatatccg catgcagctt gcggatgagg tcgcgctgct
     2041 ggtgcaacgc gaaacgggcg ttgaagctgg tcacgccctt gtggatgttg aaggtggcga
     2101 tacgtaacgt attctgcaaa ttgacgaccc ttgatccagc ctgacttcgt tgacttccag
     2161 tgcccgggtg ttttcacgcc gcgctcaggc gccttcaacc ggggatgtcg ccatgcatgc
     2221 cggcacgcat gggttgccgc tggttatgag gcttccagca tccggtcgag cgcgagcttg
     2281 gccagcttgg cttcttccgg cagtaccttg atctggttca ctacattgcc ttcggccagg
     2341 ttctccagcg tccaggccag atgctgcgga tcgatgcgcg ccatggtcga gcattcgcag
     2401 accacatgcg acatgaactg cacgatcttg ccgtccggct tgaagcgctc tgccaggcgg
     2461 ttcaccaggt tgagctcggt gccgaccagc cagcgggtgc ccggcgcgga atcggtgatg
     2521 gtcttgacga tgtattctgt agaaccgacg taatccgact gcaggcagac ctcgaacggc
     2581 gcttccgggt gcgagatgac cttgccttcc gggtgctcgt tgcggaactt gatgatgttc
     2641 tgcgggcgga acatctggtg cacggcgcag tggcctttcc acaacaggat cctggcgttc
     2701 ttgatctgtt cctcggtgag gccgcccatg ggctgatccg gatcccacac cggcatctgt
     2761 tccagcggga tgcccatctt gtaaccgctc cagcgaccca ggttctggtc ggggaagaac
     2821 agcactttct cacgtcgcgc gaagctccat tcgagcatct tggtggcgtt ggtggaggtg
     2881 cagacgatgc cgccatgctc gccgcagaag gccttgagat cggccgcgga gttgatgtaa
     2941 gtgagcggcg tcacctcttt gtcaaagtcc agcaccttgt tcagctcgcg gaaactgcgc
     3001 tcgaccttgg ccaggttggc catgtcggcc atggaacaac cggcggacaa gtcaggcaaa
     3061 atcgcgatct gctctggccg cgagaggata tcggcgacct cggccatgaa atgcacgccg
     3121 aggaagacga tgaactcggc atcgacatcg gcggcatggc gcgacagttt cagcgaatcg
     3181 ccggtgaagt gcgcgtgctt gtagacgcct tcctgctggt agtggtgccc gaggatgacg
     3241 aggcggtcgc ccagcttctc cttggccgcg acgatgcgct gctggcactc gtcgtcggcg
     3301 gagttctgga agcgttcgaa tttgatggtc gctgtttgca taatgtgatt ttacaacttc
     3361 aagtgatgcc gttcacccaa tcgtttgagt gcatcgacgt tagtccatga gaatacgcgc
     3421 ttcgccgcct cgcgccagtc cacccattca tagcgcacat gttcgtccgg tgcgagttgg
     3481 acatcgaccg gcgctggcaa ttcaagcccg aacagatgct cgacgttctc cgtcacgccc
     3541 ggcgcatagc ggtgacgcca gtgcgggtag atctcgtaag tgttcgaggc ctgccagtcc
     3601 tcgaacttgt agtccaatgc atccagcccg gtctcctccc gcacctctcg gatcgctgcg
     3661 tcataaggtg tctcgtccgc ctcgatactg cccgtcaccg actgccagaa tccgggcttg
     3721 tcggcacgct ccatgatcag cactttaagg tctggcgtat ggatcaggat cagggcggag
     3781 accggggtct tgtaactcac tattcgccct cgaactcaca caaagcgaac atcgtgtggc
     3841 ccttggcttc gagacgtttg cgaccgccaa ggctgatgag gtcgatgatg aaggcgcatt
     3901 ccaccacttc gccgccgagc ttctcgatga gcgcggcgga agccagcgcg gtgccgccgg
     3961 tggcgatgag atcatccacc agcaggatgc gttcgccttt ttcgatggcg tccttatgga
     4021 tctccacgcg gtcgctaccg tattccagct cgtaatcgtg gcccatggtc tcggccggta
     4081 gcttgccgcc tttgcggatg ggtacgaaac ccacgcccag tttgtaggcc agcggcgcac
     4141 caaccagaaa accgcgcgac tcgacgccca ccactttatc gatacgctgg tcgacatagc
     4201 gcttgtggaa ctcgtcgatg gtgaccttga ggcctagcgg gtctttcagc agcgtcgtga
     4261 tgtcacggaa ctgcacgccc tgcttggggt aa
//
LOCUS       DATMTW010000134         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_179619, whole genome shotgun sequence.
ACCESSION   DATMTW010000134 DATMTW010000000
VERSION     DATMTW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179619"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VN084_02475"
     CDS             <1..576
                     /locus_tag="VN084_02475"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWT28350.1"
                     /translation="AMLADGCGHGLAPAISVMPVLSMFYSLVETGTQPPPDLPHLVGE
                     LNRQLLATLPRGRFVAAAILCLDQRQQLADLWVGGIPALFWLGADGRVKQRFISDHLS
                     LGIVDTDADLLTLNRVHWQPGDAFVACSDGLLESQSAGGETLGVDAFVRLLEACPRDT
                     DLLEYVRAALRARLGSRAPADDISLLVVNCR"
     gene            668..1276
                     /locus_tag="VN084_02480"
     CDS             668..1276
                     /locus_tag="VN084_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWT28351.1"
                     /translation="MSMQANSTAGRMLSVLVIDESQGRAAEICAGLAIAGYQVAALVP
                     DTLDLSDRVQRLRPDVILIQTDSPSRDTLEHLAVLGRDLPRPVLMFAKERDSKVIREA
                     MRAGVSAYVVDGLQIDRLQPLVEVAMARFEEYSALRRERDEAARKLTDRIAIDRAKGV
                     LMKARGLDEEAAYAALRKLAMDRGQPIGDVASDVLAMAKILL"
     gene            1574..2818
                     /locus_tag="VN084_02485"
     CDS             1574..2818
                     /locus_tag="VN084_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWT28352.1"
                     /translation="MTEEKKGMSRREFIKASAGAGLMELLPAGEAQAWVAGSDAPEKK
                     ELRVGFIALTDCASVVMAAVQKFDEKYGIKIIPSRESSWAAIRDKLLAGELDAAQALY
                     GLVYGVQLGIGGVQKDMNILMALNNNGQGITLASRLGAEGVIDGATLAQRVARQDKSF
                     VFAQTFPTGTHAMWLYYWLATHGIDPFRDVRNIVVPPPNMVANIRGGNTDGFCAGEPW
                     NQCAINERVGFSVAATQDIWPDHPEKVLGTTEEWVQQHPNAARALTAAILEASRWIDA
                     SAANRHIAAGTIAQQAYVNTDVDVILGRMLGRYENGLGQSKDDPHAMKFHADGAVNFP
                     YLSDAMWFMTQHRRWGFLREDPDYLAVAQRVNRIDIYAQAATQLNVALPDSSMRSSRL
                     IDGVIWDGSDPTAYANSFSIHA"
     gene            2869..>3863
                     /locus_tag="VN084_02490"
     CDS             2869..>3863
                     /locus_tag="VN084_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWT28353.1"
                     /translation="MTSKSDSKNRIQSQGRRSAMFRVAALAGALSFGSLGLPSFVHAE
                     DTGIGKPEKVNLKFGFIKLTDMAPLAIAYEKGYFRDEGLNVTLEAQANWKVLLDRVID
                     GQLDGAHMLAGQPLAATVGIGTKEPIVTAFSMDLNGNAITVSNEVWKEMKPYLPMKDG
                     KVVHPIKADVLKKVIDAFKTKGKPFKMGMVFPVSTHNYELRYWLASGGISPGFYSSKD
                     VTGQTKADALLSVTPPPQMPATMESGTIDGYCVGEPWNQQAVFKGIGVPVITDDEIWK
                     NNPEKVFGVTKAWAEKYPNTNIRVVKALIRAGMWLDESMAHRIEAVKIISQSNYVG"
BASE COUNT          732 a         1242 c         1181 g          708 t
ORIGIN      
        1 gccatgcttg ccgacggctg cggccacggc ctggcgccgg cgatcagcgt gatgccggtg
       61 ctgtccatgt tctacagtct ggtcgagacg ggcacccagc cgccgcccga cctgccgcat
      121 ctggtcggcg aactcaaccg acaattgctg gcgacgctgc cgcgcggccg cttcgtcgcc
      181 gccgccattc tctgtctcga ccagcgccag cagctggccg acctgtgggt gggcggcatt
      241 ccggcgctgt tctggctcgg tgccgacggc cgcgtcaagc agcgcttcat ttccgaccat
      301 ctgtcgctgg gcatcgtcga taccgatgcc gacctgctca ccctgaaccg cgtgcattgg
      361 cagcccggcg atgccttcgt ggcctgctcc gatggcctgc tcgaaagcca gagcgcgggc
      421 ggcgaaaccc tgggcgtgga tgccttcgtg cgcctgctcg aagcctgtcc gcgcgacacc
      481 gatctgctcg aatacgtgcg cgctgcgctg cgcgcgcgcc tgggcagccg ggcgccggct
      541 gacgatatct cgctgctggt ggtgaactgc cgctgaggcg gcatcgggat cgaccaccgc
      601 cgccggtccg ggccaacggc cgcgcactct tcagaatacc ggcatgtaac ttgcatcttt
      661 gatgtgcatg tccatgcaag ccaactctac cgccggccgg atgctcagcg tcctggtcat
      721 cgacgaatcc caggggcgtg ccgccgaaat ctgcgccggc cttgccattg ccggctatca
      781 ggtggcggcg ctggtgccgg atacgctcga cctgtccgac cgcgtgcagc gcctgcgccc
      841 cgacgtgatc ctgatccaga ccgattcgcc ctcgcgcgac acgctcgaac acctggccgt
      901 gctcggccgc gatctgcccc gcccggtgct gatgttcgcc aaggagcgcg acagcaaggt
      961 aatccgcgaa gccatgcggg ccggcgtgtc ggcctatgtg gtggacggct tgcagatcga
     1021 tcgtctgcag cccctggtcg aggtggcgat ggcgcgcttc gaggagtaca gcgccctgcg
     1081 tcgcgagcgc gacgaggctg cgcgcaaact caccgaccgc atcgccatcg atcgcgccaa
     1141 gggcgtactg atgaaggcgc gcgggctcga cgaagaggcc gcgtatgcgg ctttgcgcaa
     1201 gctggccatg gatcgcggcc agcccatcgg cgatgtagct agcgatgtgc tcgccatggc
     1261 caaaatcctg ctctgagttt cacgcacgtt tccttcttca tatctctcac cataacggtg
     1321 cgcatgcacc gttatacgcc acgagagtag cggccttcgc aggcgctccg cccgcttacc
     1381 ggttcgcgtt gaaatcctct tatccctgtt gcagcagtcg tttcaagctc aatctgcata
     1441 actggcacag cgcttgctga tagggaagca cctcgaatca atggcgattt ggggcgatca
     1501 gcggacaatg gcgtccaggt gtttttcccg ttcggggaaa atgtctggcg cctttttttt
     1561 cgacttcgaa accatgacag aagagaagaa gggcatgagc agacgcgaat tcatcaaggc
     1621 atcggcaggc gccggcctga tggagttgct gccggccggc gaggcgcagg cctgggttgc
     1681 cggcagcgat gcacccgaga agaaagaact ccgggtcggc tttatcgcgt tgaccgattg
     1741 cgcttccgtg gtcatggcag cggtacagaa gttcgacgag aagtacggca tcaagatcat
     1801 tccctcgcgc gagtcttcct gggcggcgat ccgcgacaag ctgctggccg gcgaactcga
     1861 tgcggcgcaa gccctgtacg gcctggtgta tggcgtgcag ctcggcattg gcggcgtgca
     1921 gaaggacatg aacatcctga tggcgctcaa caacaacggc cagggcatca cgctggccag
     1981 ccgcctcggt gccgaaggcg tgatcgatgg cgccacgctg gcgcagcgcg tggcgcgcca
     2041 ggataaatcc ttcgtcttcg cgcagacctt tccgaccggc acccacgcca tgtggctgta
     2101 ctactggcta gccacgcacg gcatcgaccc cttccgcgat gtgcgcaata tcgtggtgcc
     2161 gccgccgaat atggtggcca atatccgcgg cggtaatacc gatggcttct gcgcgggcga
     2221 gccatggaac caatgcgcca tcaacgagcg cgtcggcttc agtgtggcgg cgacgcagga
     2281 catctggccc gaccatcccg aaaaagtgct gggcaccacc gaagagtggg tgcagcagca
     2341 tccgaatgca gcacgcgcac tgactgccgc gatactcgaa gccagtcgct ggatcgacgc
     2401 ttcggccgca aaccgccaca tcgctgccgg cactatcgcg cagcaagcct acgtcaatac
     2461 cgatgtcgat gtgattctcg gccgcatgct tggccgctat gaaaacggtc tcggccaaag
     2521 caaggacgac ccgcacgcca tgaagttcca tgccgacggt gcggtcaact tcccctatct
     2581 ctcggacgcg atgtggttca tgacccagca tcgtcgttgg ggtttcctgc gcgaagaccc
     2641 tgattacctg gccgtggcgc agcgcgtcaa tcgcatcgat atctatgcgc aagccgcgac
     2701 gcaactgaat gtggcgctgc ccgacagcag catgcgcagc agccgattga tcgacggtgt
     2761 gatctgggac ggcagcgatc cgaccgctta tgccaattcc ttttcgattc acgcctgacc
     2821 cgcttcgccg ttacacatta cgccccgttt tttaacttgg agaacgcaat gacttctaag
     2881 tccgacagca aaaaccgcat ccagtcgcaa ggccgccgca gtgccatgtt ccgtgttgcc
     2941 gccctggccg gcgcgctcag cttcggcagc cttggcttgc cgagcttcgt gcatgcggaa
     3001 gatacgggca tcggcaagcc cgaaaaagtt aatctgaagt tcggcttcat caagctgacc
     3061 gacatggcgc cgctcgccat cgcctacgaa aagggttact tccgcgatga aggcctgaac
     3121 gtgacgctgg aagcccaggc caactggaag gtactgctcg accgcgtcat cgacggtcag
     3181 ctcgacggtg cccacatgct ggccggccag ccgctcgccg ctaccgtcgg catcggcacc
     3241 aaggagccca tcgtcactgc cttcagcatg gacctcaacg gcaatgcgat taccgtgtcc
     3301 aacgaagtgt ggaaggaaat gaagccctac ctgcccatga aggatggcaa ggtggtgcac
     3361 ccgatcaagg ccgatgtgtt gaagaaggtg atcgacgcct tcaagaccaa gggcaagccc
     3421 ttcaagatgg gcatggtgtt cccggtgtcc acccacaact acgagctgcg ctactggctt
     3481 gcttccggcg gcatcagccc cggcttctat tcgagcaagg atgtgactgg ccagaccaag
     3541 gccgacgcgc tgctgtcggt aacgccgccg ccgcaaatgc cggcgaccat ggagtccggc
     3601 accatcgacg gctactgcgt gggcgagccc tggaatcagc aggccgtgtt caagggcatc
     3661 ggcgtgccgg tgatcaccga cgacgaaatc tggaagaaca atccggaaaa ggtcttcggc
     3721 gtgaccaagg cctgggccga gaagtatccg aacaccaaca tccgcgtagt caaggcgcta
     3781 atccgtgccg gcatgtggct ggatgaaagc atggcccacc gcatcgaagc cgtgaagatc
     3841 atttcgcaat ccaactacgt cgg
//
LOCUS       DATMTW010000135         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_113049, whole genome shotgun sequence.
ACCESSION   DATMTW010000135 DATMTW010000000
VERSION     DATMTW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113049"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1249
                     /locus_tag="VN084_02495"
     CDS             47..1249
                     /locus_tag="VN084_02495"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWT28354.1"
                     /translation="MAVERVLSGMRPTGNLHLGHYHGVLKNWIRLQHEHECFFFVADW
                     HALTTHYDTPEIIEESVWEMVIDWLAAGVDPASATIFIQSRVPEHAELHTLLSMITPL
                     SWLERVPTYKDQQEKLASKDLSTYGFLGYPLLQSADILIYRATQVPVGEDQIPHIEFT
                     REIARRFNHIYGREPGYEEKAEAAIKKLGSKRSALYEEMRNAYQQSGDEEALESARAM
                     IEEQQGLSLGDKERLLGYLEGGGKIILPEPEYKLTPASKMPGLDGQKMSKSYNNTVSL
                     REPADVVAKKIKTMPTDPARIRRTDPGDPAKCPVWQLHEVYSDDARREWVMQGCTTAG
                     IGCIECKQPVIDAMMEELKPIQERAAQYAEDPLLVKNIVAEGCERARKLARETMRDVR
                     EAMGLNYS"
     gene            1343..2134
                     /locus_tag="VN084_02500"
     CDS             1343..2134
                     /locus_tag="VN084_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWT28355.1"
                     /translation="MQTQLTIDARIYGEQLDTIPQDLYIPPDALEVYLDAFEGPLDLL
                     LYLIRKHNLDILDIPMAELTRQYMDYVEKMRAMSEHFKLELAADYLLMSAMLIEIKSR
                     MLLPKPEEIVEEDDPRAELVRRLMEYEAIKLAAQRLDAMPQVGVDILVAQSYVERISD
                     IHPPEVSVDDLVAAWKGVLARAGNFRHHRISREELSVREHMSDVLRRLQEHRMLEFTA
                     LFDVVNDGIPKLVVYFLAILELAREGLLRITQQQAFTPIYLQIAA"
     gene            2143..2697
                     /gene="scpB"
                     /locus_tag="VN084_02505"
     CDS             2143..2697
                     /gene="scpB"
                     /locus_tag="VN084_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWT28356.1"
                     /translation="MQHEPQTIEEMKQVLEAALLTSAQPLSLDELLRLFAGRMERATL
                     RMLLDELKSDWQARTLELVQVASGWRFQARSQYAPFIARLNPEKQPKYSRAVMETLAI
                     IAYRQPVTRGDIEEIRGVAVNPNVMRQLQERDWIDVVGQRDTPGRPSLYGTTRTFLDD
                     LNLRSLAELPPMEDFAQTEIPLDL"
     gene            2780..>3060
                     /locus_tag="VN084_02510"
     CDS             2780..>3060
                     /locus_tag="VN084_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWT28357.1"
                     /translation="MARPFKQQPTQRIKPAAVDPDAVPQRLHKLLALAGLGSRREMET
                     LIESGRVTINGKPAQVGAGVVPSDVVRVDSRPVRLPFEPELPQVLLYHKP"
BASE COUNT          632 a          939 c          913 g          576 t
ORIGIN      
        1 ctttgtataa tgcagctatc tttttccgaa agactataaa aacatcatgg cggttgaacg
       61 cgttttatcg ggcatgcgcc ccacaggtaa cttgcatctg gggcattatc acggggtatt
      121 gaagaactgg atcagattgc agcatgagca cgaatgcttc ttcttcgtcg ccgactggca
      181 cgcgctgacc acccattacg acacgccaga gatcatcgag gagagcgtct gggagatggt
      241 catcgactgg ctcgctgccg gggtcgatcc cgccagcgcc accatcttca tccagtcgcg
      301 tgtgccggag catgccgagc tgcacacgct gttgtccatg atcacgccgc tgtcctggct
      361 ggaacgcgtg ccgacctaca aggaccagca ggagaaactg gcgagcaagg atctctcgac
      421 ctacggcttc ctcggttatc cgctgttgca gagcgccgac atcctgatct accgtgcgac
      481 gcaggtgcct gtgggcgagg accagatccc gcatatcgag ttcacccgcg agatcgcccg
      541 tcgcttcaac catatctatg gtcgtgaacc gggttacgaa gagaaggccg aagccgccat
      601 caagaagctg ggcagcaagc gcagcgcgct ctatgaggag atgcgcaacg cctaccagca
      661 aagtggcgac gaagaggcac tggaatcggc acgcgccatg atcgaagagc agcagggctt
      721 gtcgctgggc gacaaggagc gcctgctggg ctatctcgaa ggcggcggca agatcatcct
      781 gccggaaccc gaatacaagc tgacgccagc ctccaagatg ccggggctgg acgggcagaa
      841 gatgtccaag tcctacaaca acaccgtgtc gctgcgcgaa ccggctgatg tcgttgcgaa
      901 gaagatcaag accatgccga ccgaccctgc gcgtatccgt cgtaccgatc cgggcgatcc
      961 ggccaagtgc ccggtctggc agttgcatga ggtctattcc gacgacgccc gccgcgaatg
     1021 ggtgatgcag ggctgcacca ccgccggtat cggttgtatc gaatgcaaac agccggtgat
     1081 cgacgccatg atggaagagc tgaagccgat ccaggagcgg gctgcgcaat acgccgaaga
     1141 ccccttgctg gtgaagaaca tcgttgccga aggctgtgag cgcgcacgca agctggcgcg
     1201 tgagaccatg cgcgacgtac gtgaagccat gggcctcaac tactcctgat gtcacgatga
     1261 gacgccacgc catgcctgag acattagccg cgtcgacgga tggcgggcct gccgtttcac
     1321 tggatggtga ggcgtgagca ccatgcagac ccagttgacg atcgatgcgc ggatctatgg
     1381 tgagcagctc gacaccatcc cgcaggatct ctacatcccg ccggatgcgc tggaagtcta
     1441 tctcgatgcc ttcgagggcc cgctggacct gctgctgtac ctcattcgca agcacaatct
     1501 cgatatcctc gacatcccca tggccgaact cacgcggcaa tacatggatt acgtggaaaa
     1561 gatgcgcgcc atgtccgagc acttcaagct ggaactggcc gctgattacc tgctgatgtc
     1621 ggccatgctg atcgagatca agtcgcgcat gctgttgccc aagcccgaag agatcgtgga
     1681 ggaggatgac ccgcgggccg agctggtgcg caggctcatg gagtacgagg cgatcaagct
     1741 cgccgcgcag cgtctcgatg ccatgccgca ggtcggtgtc gatatcctgg tcgcccagag
     1801 ttacgtcgag cgcatcagcg atatccaccc gccggaagta tcggtcgatg atctggtcgc
     1861 ggcctggaag ggcgtgctgg ctcgcgccgg taattttcgt catcacagga tcagccgcga
     1921 agagctctcg gtgcgcgagc acatgagcga cgtcctgcgg cgcctgcagg aacaccgcat
     1981 gctcgagttc acggcgctgt tcgacgtcgt taacgacggc attcccaagc tggtcgtgta
     2041 tttcctcgcc atcctcgaac tggcgcgcga aggtctgcta cgtatcaccc agcagcaagc
     2101 cttcaccccc atctatctgc agatcgcagc ctgaggtcgt cgatgcagca cgaaccacaa
     2161 accatcgaag aaatgaagca ggtgctggaa gccgcattgc tcaccagtgc gcagcccttg
     2221 tccctggatg agttgctgcg cctgttcgcc ggtcgcatgg agcgcgcgac cttgcgcatg
     2281 ctgctggatg aattgaagag cgattggcag gcgcgcacgc tggaactggt gcaggtcgcc
     2341 agcggctggc gcttccaggc acgtagccag tatgccccat ttatcgctcg gctcaacccg
     2401 gagaagcagc ctaaatattc gcgcgccgtc atggaaacgc tcgccatcat cgcctatcgc
     2461 caaccggtga cgcgcggcga tattgaggag atccgcggtg tcgcggtcaa ccccaacgtc
     2521 atgcgccaat tgcaggagcg cgactggatc gatgtcgtcg gccagcgcga cacgccgggc
     2581 cggccgtcgc tctatggcac caccaggacc tttctcgacg acctcaatct gcgctcgctc
     2641 gccgaattgc cgccgatgga agatttcgcg cagaccgaga tcccgctgga cttgtaactt
     2701 tctctcgata tttcatcgtc ttattcgtgc ctggccgtgc tctggcttat aataggagcc
     2761 ttcatacgaa gtagcagcca tggcccgtcc tttcaagcag caacctaccc aacgcatcaa
     2821 acccgccgcc gtcgatccgg acgccgtccc acagcgcctg cataaactgt tggcgctggc
     2881 tggccttggt tcgcgtcgtg agatggagac cctgatcgag agcggtcgcg tcaccatcaa
     2941 cggcaaaccc gcccaggtgg gcgcgggtgt cgtgcccagc gatgtggtgc gggtcgatag
     3001 tcggccggtg cgcctgccgt tcgaaccgga attgccgcag gtgctgctct accacaagcc
//
LOCUS       DATMTW010000136         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_113075, whole genome shotgun sequence.
ACCESSION   DATMTW010000136 DATMTW010000000
VERSION     DATMTW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113075"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VN084_02515"
     CDS             <1..609
                     /locus_tag="VN084_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HWT28358.1"
                     /translation="YLARNFLAFFRRDYREVAKAHVESGWVPADTPIDEFETAIRAIC
                     EPIFDKPLKEISFGRTLLALFQMARRFGVIIQPQLIMLQKTLLNIEGLGRDLDPDLDL
                     WKTARPFLERWMSEQVGWRSVLQNIKAELPFWGKILPQMPRMLHQQLQRDHDTPINQE
                     LLALVREQRRHSMWLRVIAIGLTLIVLFQGFSLYVIWFGMAH"
     gene            706..2190
                     /locus_tag="VN084_02520"
     CDS             706..2190
                     /locus_tag="VN084_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWT28359.1"
                     /translation="MLIWFVVAYLMLSIGIGLYAATRVKNTKDYAVAGRHLPLPVVMA
                     TVFATWFGAEAVFGVSATFVKEGLNGVVADPFGSSLCLVIAGFFFATQLYKLNIITLG
                     DFYRMRYNRTVEVLTTIAIVISYLGWVAAQIKALGLIFSMLTNGAISQEAGMILGTAI
                     VLTYTTFGGMLSVAILDFVQMIVVIGGLLFIGYIVSGMTGGVAPVIEHARAAGKLDFF
                     PKGGDIWVWVTFIGAWMTMMLGSIPQQDVFQRITSAKSAKIAVWGSVLGASVYFCFTF
                     VPMFIAYSATLIDPGLFNTLINEDSQRVLPTLVLQHTPLLAQAIFFGAVISAIMSCSS
                     ATLLAPSISFAENIVRGYMPHLSDRGFLKVMRICLVGFACCVLLYALNSERSIFGMVE
                     SAYKITLAGAFVPLFFGAFWKRATNQGALAAIIGGIAAWVLIEVLVGEDSLIPPQLIG
                     LGVSVVGMVAGSLLPQKIAGHTNPPLGYLHAHAAHVHTPETHHH"
     gene            complement(2237..3049)
                     /gene="mutM"
                     /locus_tag="VN084_02525"
     CDS             complement(2237..3049)
                     /gene="mutM"
                     /locus_tag="VN084_02525"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWT28360.1"
                     /translation="MPELPEVEVTRRGMLPLLQQQVREVVIRNPALRWPIPADLPQLL
                     AGSRLQSLDRRGKYILARFDSGTVILHLGMSGRLCLLARDEAPAKHDHFDIHFTGGQV
                     LRLRDPRRFGAVLWAGAQPHKHVLLEALGPEPLEAAFTGDRLYRETRSRSAPIKNVIM
                     DSHLVVGVGNIYASESLFRAGIHPNTPANKLSRARCERLVTEIKATLQDALEAGGSSL
                     RDFFGADGNPGYFQQAYFVYGRTGQPCKVCGKSIRLLRLGQRSTFYCTTCQR"
     gene            complement(3065..3943)
                     /locus_tag="VN084_02530"
     CDS             complement(3065..3943)
                     /locus_tag="VN084_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWT28361.1"
                     /translation="MKSRWLMNLAMLVLIVGIVAFLYLRPKAVDNDTPTYEVSTLKMA
                     DFDHVTVEFPTKAPVAFEKIGGYWYLVKPYKARAEQRMVQAILSIIAATTTHKFPADD
                     LARFGLDTPKLKVKHNDQEFVFGTYNTLTSEQYVAYKGALYLLPVTYSEAASTQVVEM
                     LEKNPLAPGEKIAGFDLSHLEQWEDIRLIVDLVDGKWKASAANAKPQQNEMNEWFETS
                     WKHPAVLSVEPYTPDRKTTYPSLEVKLADGRKVHLDKLQESPELLLGRPDEGMLYHFP
                     QDAGFVMLNPPINLSK"
     gene            complement(3953..>4187)
                     /locus_tag="VN084_02535"
     CDS             complement(3953..>4187)
                     /locus_tag="VN084_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HWT28362.1"
                     /translation="ITNGGNLDLGVNVVNWLTNDDKLITIQPMVQKDINMTIPSTGMG
                     ALVALIIGYGFQIVLPALFLIFGFVVWFKRRKA"
BASE COUNT          772 a         1294 c         1224 g          897 t
ORIGIN      
        1 tacttggcgc gcaacttcct agctttcttc cggcgtgatt accgtgaagt ggccaaggcc
       61 cacgtcgagt ccggctgggt accggcagac acgccgatcg atgaattcga gaccgcgatc
      121 cgcgccatct gcgaaccgat cttcgacaag ccgctgaaag agatctcctt cgggcgcacc
      181 ttgctggcct tgttccagat ggcccgccgc ttcggtgtca tcatccagcc gcaactcatc
      241 atgctgcaga agaccctgct caatatcgaa ggcctgggcc gcgacctcga cccggatctc
      301 gacctgtgga agactgcccg ccccttcctg gaacgctgga tgtcggaaca ggtcggctgg
      361 cgcagcgtgc tgcagaacat caaggccgag ctgccgttct ggggcaagat cctgccgcag
      421 atgccccgca tgcttcacca gcaacttcaa cgcgatcatg acacaccgat caatcaggag
      481 cttctggccc tggtacgcga gcagcgacgc catagcatgt ggctgcgcgt gatcgccatc
      541 ggcctgaccc tgatcgtgct cttccaggga ttcagcctgt acgtgatctg gttcggcatg
      601 gcgcactgat cgtcgcgctg gccgctcgca aggcagctac tgcgaatccg ccgcttttgc
      661 tagaatccac gcatcgaatg tcttctgcgt gaaggatctt ccaccgtgct catctggttc
      721 gtcgtcgctt atctgatgct gtccatcggc atcggcctct atgccgccac tcgcgtcaag
      781 aacaccaagg attacgccgt cgccggccgg cacttgccgc tacctgtggt catggccacc
      841 gtcttcgcca cctggttcgg tgccgaggct gtgttcggcg tctcggccac gttcgtcaag
      901 gaaggcctca atggcgtagt cgccgacccc ttcggctcaa gcctttgtct cgtcatcgcc
      961 ggcttcttct ttgccaccca gctatacaag ctcaacatca tcacgctcgg cgacttctat
     1021 cgcatgcgtt acaaccgtac agtagaagtg ctgaccacca tcgccatcgt catctcttac
     1081 ctgggctggg tcgccgccca gatcaaggcg ctcgggctca tcttcagcat gctcacgaac
     1141 ggcgccatca gccaggaagc cggcatgatc ctgggcacgg ccatcgtgct cacctacacg
     1201 acgtttggcg gcatgctgtc ggtggccatc ctcgatttcg tgcagatgat cgtggtcatc
     1261 ggcggcctgt tattcatcgg ctacatcgtc tccggcatga ccggtggcgt ggcgcctgtg
     1321 atcgagcatg cccgcgccgc cggcaaacta gacttcttcc cgaagggcgg ggatatctgg
     1381 gtctgggtca cgttcatcgg tgcgtggatg accatgatgc tggggtcgat cccacagcag
     1441 gatgtattcc agcgcatcac ctcggcgaaa agcgcgaaga tcgctgtctg gggctcggtg
     1501 cttggggctt cagtctattt ctgcttcact ttcgtgccga tgttcatcgc ctactccgcc
     1561 acactgatcg atccgggcct tttcaacacc ctgatcaacg aagattcgca gcgcgtgttg
     1621 ccgacgctgg tgctgcaaca cacacccctg ctggcgcagg cgatcttctt cggcgcggtc
     1681 atctcggcca tcatgagttg ttcgtcggca acgttgctgg cgccttcgat ctcgttcgcc
     1741 gagaacatcg tgcgtggcta catgccgcac ttgagtgacc gcggcttcct caaggtcatg
     1801 cgcatctgcc tcgtcggttt cgcctgctgc gtgttgctgt atgcgctgaa ctcggaacgg
     1861 tcgatcttcg ggatggtgga gtcagcctac aagatcacac tggcgggagc cttcgtgccc
     1921 ttgttcttcg gcgccttctg gaaacgcgcc accaatcagg gtgcgctggc ggcgatcatc
     1981 ggcggcatcg ccgcctgggt gttgatcgag gtattggtcg gggaagacag cctgataccg
     2041 ccacagctca tcggcctcgg cgtcagcgtg gtcggcatgg tggccggctc attgctaccg
     2101 cagaagatcg cggggcacac caacccgccg ctgggttacc tgcatgccca tgcagcccat
     2161 gtgcacacgc cagagacgca ccatcactag ccggcagtga ccgcctcatg gcgcatgcgc
     2221 ttaccgccga tcagcctcag cgctggcagg tcgtgcaata gaaggtcgag cgctgaccga
     2281 gtcgcaggag cctgatcgac tttccgcaga ccttgcacgg ttggccggtg cggccataga
     2341 cgaaataggc ttgctggaaa tagcccgggt tgccatccgc cccaaagaaa tcgcgcaaac
     2401 tgctgccgcc tgcttccagc gcatcctgca gcgtcgcctt gatctccgtc accagccttt
     2461 cgcagcgcgc gcggctgagc ttgttggctg gcgtattcgg atggatccct gcgcggaaga
     2521 gcgattcact cgcgtagata ttacccaccc ccaccaccag gtggctgtcc atgatgacgt
     2581 tcttgatcgg cgcgctgcgg ctgcgagtct cgcggtaaag ccgatccccg gtgaacgcgg
     2641 cctcaagcgg ttcaggcccc aaggcttcca gcaacacgtg tttgtgcggt tgagcgcctg
     2701 cccacaatac ggcgccgaag cgacgcgggt cgcgcaggcg caggacttgc ccgccagtga
     2761 aatggatgtc gaaatggtcg tgtttggcgg gcgcctcgtc ccgcgcgaga agacagagac
     2821 ggccggacat gccgaggtgc aggatcaccg taccggaatc gaaacgtgcg aggatatatt
     2881 tgccgcggcg gtcaagcgat tgtagtcggc tgccggcaag cagctgtggc aaatcagcag
     2941 gaatcggcca gcgcaacgca ggattgcgga tcacaacttc gcgcacctgc tgttgcagca
     3001 ggggcagcat gccgcgtctg gtgacttcga cctctggtaa ttcaggcatg ccggcgcgcc
     3061 ggccttattt ggacagattg atcggcggat tgagcatcac gaagccggcg tcctgaggga
     3121 agtggtacaa catgccttcg tccggacgcc ccagtagcaa ctccggcgat tcctgcagct
     3181 tgtccagatg caccttacgg ccatccgcca gcttcacctc gagcgatggg tatgtagtct
     3241 tgcggtccgg cgtataaggc tcgacggaga gtacggctgg atgtttccag ctggtctcga
     3301 accactcgtt catctcgttc tgctgcggct tggcgttcgc ggccgatgct ttccatttgc
     3361 catcgaccag atccactatc aggcggatat cttcccattg ctcgagatgc gacaggtcga
     3421 aaccggcgat cttttcgcca ggtgccaggg ggttcttttc cagcatctcg accacttggg
     3481 tagaggccgc ttcggagtaa gtcaccggta gcagatagag ggcaccttta taagcgacgt
     3541 actgctcgga ggtcagcgtg ttgtaagtcc cgaacacgaa ctcctggtca ttgtgcttga
     3601 ccttgagctt gggcgtatca agaccgaaac gggcaaggtc atcagcagga aacttgtgcg
     3661 tagtggtcgc ggcgatgatc gagaggatag cctgcaccat gcgctgctct gcccgggctt
     3721 tgtagggttt caccaggtac cagtagccgc cgatcttctc gaaggctacc ggcgccttgg
     3781 ttgggaattc gaccgtgaca tgatcgaaat ccgccatctt cagggtcgaa acctcatagg
     3841 tcggggtatc gttatcgacc gccttcggtc gcaggtaaag gaaggcgacg atgccgacga
     3901 tcagcaccag catcgcgaga ttcatcaacc agcgtgactt catgacgacc tcttacgcct
     3961 ttctgcgctt gaaccagacc acgaacccaa agatcaggaa cagggccggc agcacgatct
     4021 ggaacccgta gccgatgatc aatgccacca gcgcgcccat acccgtgctg gggatcgtca
     4081 tattgatgtc tttctgcacc atcggctgga tcgtgatcag tttgtcgtcg ttggtcagcc
     4141 agttgacaac gttgacgccc agatcgaggt tgccgccgtt ggtgatg
//
LOCUS       DATMTW010000137         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_380917, whole genome shotgun sequence.
ACCESSION   DATMTW010000137 DATMTW010000000
VERSION     DATMTW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380917"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1098)
                     /locus_tag="VN084_02540"
     CDS             complement(94..1098)
                     /locus_tag="VN084_02540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWT28363.1"
                     /translation="MNTHSSDLTHPGEQAQRRLLDTSALRLALGRFATGLTVATATGP
                     DGRLAGITVNSFNAVSLDPPLVLWSLARHAGSAPVFEAASHWAVHVLAHDQSDLAAHF
                     ARSNPDKFAGLAYRRSDDGTPLLSGCAARFLCRAVHRYDGGDHIIMVGRVLQFEQSTT
                     PALVYHDSAYAIATSTAEHEAAEPESPAAGSLGFLLGSAFFYMYGRLRDAGGKLGFTN
                     SELFVLMALGERGWRSRGEINALLAYGGHPTNLQVLDDLEGSGRCTSRPGISGATDDA
                     QFALTPAGEVVVAELTRASLAVMADMDDILGMSGSIALRSLLRRFVKDTESSRTVNWL
                     "
     gene            1404..>2169
                     /locus_tag="VN084_02545"
     CDS             1404..>2169
                     /locus_tag="VN084_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28364.1"
                     /translation="MLTLVRNDGPHENADMEGLTALPSAPVADDRAELLNRTIHAIYQ
                     SVLESEPWLACLKLMVEYLDTNDAIIVVRPSTELELGYLVCFPSDPEVELEYRSVWYK
                     VDPFVELPLERVVTVADVMSDQQWRSCDFYRHFFGDMWSVDATRVMGVNITTRAGTVS
                     RLRLHRLQDAAPFSPEDKQRLAALIPHIKQAMTLAAHLNRNESEREIYEEGLDRLNIG
                     VIVLDETGQLLRANPVACHILNGADGVKLVDRHLEGC"
BASE COUNT          440 a          622 c          675 g          432 t
ORIGIN      
        1 acggctccgt tttatatcac gatttgtatc gtagccgtcg ggctcatcag tgcgtgcctg
       61 atgagcaaga atgggtcgga cgactgaacc aggctatagc cagttcacgg tacgtgacga
      121 ttcggtgtct ttcacaaagc gtcgcagcaa gctgcgcaag gcaatgctcc ctgacatccc
      181 gaggatatcg tccatgtctg ccatcaccgc gaggctggcc cgggtcaact cggcaaccac
      241 cacttcaccg gccggtgtca gggcaaactg ggcatcgtcc gtggcgccgc tgatccccgg
      301 gcgggacgtg caccgcccgc tcccttcgag atcgtccagc acctgcaggt tggtcgggtg
      361 gccgccgtac gccagcagtg cgttgatttc gccgcgactg cgccatccgc gttcacccag
      421 cgccatgagg acgaacaact cgctattggt aaatcctagc ttgccgccgg cgtcgcgcag
      481 cctgccgtac atgtagaaga acgcggagcc gagcaggaag ccgagggagc ctgcggcagg
      541 gctttcgggc tccgcggcct cgtgttccgc cgtactggtg gcaatcgcgt aagcactgtc
      601 gtgatacacg agtgccggtg tcgtactttg ctcgaattgc aggacccggc cgaccatgat
      661 gatgtgatcg ccgccatcat agcgatgcac ggcacggcac aggaagcgtg cggcgcagcc
      721 gctcagcaag ggcgtgccgt cgtcgctgcg cctgtacgcc aggccggcga acttgtcggg
      781 gttcgatctg gcgaagtgcg cagccaggtc ggactggtcg tgagccagta cgtggaccgc
      841 ccagtgcgag gcggcctcga ataccggcgc gctgcccgcg tggcgtgcga ggctccacaa
      901 caccaggggc gggtccaggg agaccgcgtt gaagctgttg acggtaattc ccgccagacg
      961 cccatcgggt cccgtggcgg tggccacggt gagcccggtg gcgaagcggc caagggccag
     1021 gcgcaatgca ctggtgtcca gcaagcgacg ctgagcttgt tcgccaggat gcgtcaagtc
     1081 cgagctatgt gtattcattc gtagactccg tgtaccttgg cgccggctgc caggttctcg
     1141 atgcccatta tggatagttt ttcgctccgg acatcgtcga accggactag gtaagaccga
     1201 cggcagcggc agcgaggctg ggctagtcca ttcggatgat gcgcgccaga tgcgtgacaa
     1261 cgagactagg tcacaaggtg gacgatgtaa ccaccagcct catcccaaag caggttactg
     1321 ggtgcgtaca catggatagc aatctggcaa aatagtgatg taaatatata aaagtggcct
     1381 taaagctagc tagggagaca gtcatgctca ctcttgtgag gaacgacgga ccacatgaaa
     1441 atgccgatat ggagggactg acagctttgc cttcggcccc tgttgcggat gatcgagcgg
     1501 aactgcttaa ccgcacaatt catgcgattt atcagtcggt gcttgaaagc gagccgtggc
     1561 tcgcctgcct caaattgatg gtcgaatacc tggataccaa cgatgccatc atcgtggtcc
     1621 ggccctccac cgaacttgaa cttgggtacc tggtatgttt tcctagcgac cccgaggttg
     1681 agctggaata tcgctccgta tggtacaagg tcgacccctt cgtggaattg cccctggaac
     1741 gcgtggtgac ggttgccgac gtgatgagcg accaacaatg gcgcagctgc gatttttacc
     1801 ggcatttttt tggcgatatg tggagtgtcg atgccacgcg tgtcatggga gtaaatatca
     1861 ccacgcgggc tggaacggtt tcacgtttgc gtttgcatcg cctgcaggac gcggcgccat
     1921 tctcgcccga ggacaagcag cggctcgcag cattgattcc gcatatcaag caagccatga
     1981 cgctcgcggc ccatctgaac cgcaacgaat ccgaacgcga aatctatgaa gaaggactcg
     2041 accggctgaa cattggcgtg atcgtgctgg acgaaacggg gcagctgttg cgggccaatc
     2101 cggtcgcatg tcacatcctc aacggtgctg atggggtcaa gctggtggat cgccatctgg
     2161 agggctgca
//
LOCUS       DATMTW010000138         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_145468, whole genome shotgun sequence.
ACCESSION   DATMTW010000138 DATMTW010000000
VERSION     DATMTW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145468"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..477)
                     /gene="panD"
                     /locus_tag="VN084_02550"
     CDS             complement(97..477)
                     /gene="panD"
                     /locus_tag="VN084_02550"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWT28365.1"
                     /translation="MQRTMLKSKLHRVRVTHSELDYEGSCAIDEALLDAADIREYQQI
                     DIYNINNGERFTTYAIRAQRGSGTISVNGAAARKASPGDLLIIASYASYNEIELEKFK
                     PQLVYVDAENRIKDQRHAIPVQAA"
     gene            complement(546..1379)
                     /gene="panC"
                     /locus_tag="VN084_02555"
     CDS             complement(546..1379)
                     /gene="panC"
                     /locus_tag="VN084_02555"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWT28366.1"
                     /translation="MHVVSTVAELRQRLGSETSVAFVPTMGNLHAGHLHLVELAKQHA
                     GTVVVSIFVNPLQFGANEDLANYPRTPEADYEKLKQAGADIVFTPTAEEMYPTTQTLL
                     VEPPPIANELCGAARPGHFKGVATVVLKLFNMVQPQVAVFGKKDFQQLFVIREMVRQL
                     NLPITIVAGETMREPDGLAMSSRNGYLQPAQRVEAPRLHRALQQVVTAMQAGEQDPVT
                     IEAQTTQYLTQLGWIVDYISVRAANTLQPAQADDRELVVLGAARQGRTRLIDNLEFTR
                     D"
     gene            complement(1379..2164)
                     /gene="panB"
                     /locus_tag="VN084_02560"
     CDS             complement(1379..2164)
                     /gene="panB"
                     /locus_tag="VN084_02560"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWT28367.1"
                     /translation="MNLTALQQMAASGDKIAVLTAYDASFATLCARAGVDVLLVGDSL
                     GMVLQGADSTLAVTLHDMQYHTRCVAKGAAGAYIVTDLPFGSYQQSPEQAFRNAARLM
                     ASGAQMVKLEGGAVMTETVRFLVERGIPVCGHLGLTPQSVHQLGGYRVQGKDQAEAQR
                     LVADAQALADAGAGMLVLEMVPASLAKTITESVRIPTIGIGAGVDCSGQVLVLHDMLG
                     IYPGKSPRFVRNFMQGAPSIAQALEDYVKAVKSKTFPTAEHSF"
BASE COUNT          404 a          656 c          723 g          466 t
ORIGIN      
        1 cgtccatgac ggtgatgtca aaagccttga tatcttccag cgcgtcgatg acggccagtt
       61 tggtttgttc caggtctagc aaaagagggt ctgctttcag gcggcttgca cggggatggc
      121 gtgtcgctgg tccttgatgc ggttctctgc atcgacatag accagctgtg gtttgaattt
      181 ttccagctcg atctcgttat agctggcata gctggcgatg atcagcagat cgccgggact
      241 ggccttgcga gctgcggcac cgttgaccga gatggtgccg gagccgcgtt gggcacggat
      301 ggcgtacgtg gtgaagcgtt cgccgttgtt gatgttgtag atatcgatct gctgatactc
      361 gcggatatcg gcggcgtcca gcagggcctc gtcgatggcg caggagccct catagtccag
      421 ctccgaatgc gtcacgcgca cgcggtgcag cttggatttg agcatggttc tttgcatcgg
      481 ctacctaatt gattctaaaa ggatggctat tatagtcagt tcgggtccgg ggtgcaaaaa
      541 caccttcagt cacgtgtgaa ctcgaggttg tcgatgaggc gagtgcgccc ctggcgggcc
      601 gccccgagca cgaccagctc acggtcatcc gcttgcgccg gttgcagggt attcgccgcg
      661 cgtaccgaga tgtagtcgac gatccaaccc agttgcgtga ggtattgcgt cgtctgggct
      721 tcgatcgtca cgggatcctg ctcgcccgct tgcatggccg tgaccacttg ctgcagggcg
      781 cgatgcagcc gcggtgcctc aacccgctgc gccggttgca gatagccgtt gcgcgaactc
      841 atggccaggc cgtcaggctc tcgcatggtc tcgccggcga cgatagtgat cggcaggttc
      901 aactggcgca ccatctcacg gatgacgaag agctgctgga aatccttctt gccgaacacc
      961 gccacctgcg gctgtaccat attgaagagc ttgagcacga cggtcgcgac acccttgaaa
     1021 tggcctgggc gggctgcgcc acagagctcg ttggcgatcg gcggcggctc aaccagcagc
     1081 gtctgcgtgg tcgggtacat ctcttccgcg gtcggcgtaa agacgatatc ggcgccggcc
     1141 tgcttgagct tttcgtaatc cgcttccggg gtgcgagggt aattggccag gtcttcgttg
     1201 gcgccgaatt gcagcggatt gacgaagatg ctgaccacca cggtcccggc gtgttgcttc
     1261 gccagttcaa cgagatggag atggcctgca tgcaggttgc ccatggtggg cacgaaagcg
     1321 acggaggttt cgctgccaag acgctgacgc agttctgcga ccgtactcac cacgtgcatc
     1381 agaagctgtg ctccgccgtg gggaaggtct tgctcttcac cgccttgacg taatcctcga
     1441 gcgcttgcgc gatggagggt gcgccttgca tgaaattgcg cacgaagcgc ggcgacttgc
     1501 cgggatagat gccgagcatg tcatgcagga caagcacctg accgctgcaa tcgacgccag
     1561 caccgatgcc gatggttggt atccgcacac tttcggtgat ggtcttggcg agcgaggcgg
     1621 gcaccatctc cagcaccagc atgccagcac cggcatcagc cagcgcctgc gcatcggcga
     1681 ccaatcgctg cgcctctgct tgatccttgc cttgcacgcg gtatccgccg agctggtgca
     1741 cggattgggg tgtcaggccc aggtggccac acaccgggat gccgcgttcg accaggaaac
     1801 gcacggtctc ggtcatgacc gcaccgcctt cgagcttgac catctgtgcg ccagaggcca
     1861 tcaggcgtgc ggcattgcga aaggcctgtt ccggactctg ctgataactg ccgaagggca
     1921 agtcggtgac gatgtaggcg ccagcagccc ccttggcgac acagcgcgta tggtattgca
     1981 tgtcatgcag ggtcacggcg agcgtgctgt cggcgccttg caacaccatg cccagtgagt
     2041 cacccaccag cagcacatcg acgccggcgc gggcgcagag ggtggcgaaa ctggcgtcat
     2101 aggcggtcag tacggcgatc ttgtcgccgc tggcggccat ctgctgcagg gcggtcaggt
     2161 tcatgaaggc tcagtcgtcc agattgggat tgaagaactc gcgccgccca cgtatctgca
     2221 tgatgcgttg caccagcaga tcgagcgcg
//
LOCUS       DATMTW010000139         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_414622, whole genome shotgun sequence.
ACCESSION   DATMTW010000139 DATMTW010000000
VERSION     DATMTW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414622"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN084_02565"
     CDS             complement(<1..249)
                     /locus_tag="VN084_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWT28368.1"
                     /translation="MAKYAHLAGRILLALIFVIAGIGKFNDPAGTTAYMGSVGLPAIL
                     LWPTILLEVLGGIAIIVGFQTRLVSYLLAGFTLLAAALF"
     gene            365..952
                     /locus_tag="VN084_02570"
     CDS             365..952
                     /locus_tag="VN084_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWT28369.1"
                     /translation="MHIIGILLAGGHSRRFGSNKLLHPMADGQPVALVAARHLLAALP
                     HSIAIVRDDTPLGNLLKAAGMQVIECTTAQQGMGDSLAAAVRFAAAQGEVDGFVVALA
                     DMPYIQPQTITAVADALAGGAAIALPVHAGQRGHPVGFAARFSDALQALAGDEGARAI
                     VQANRSMVLEIAVEDAGILRDIDYPTDLQTDPAAD"
     gene            complement(1028..2038)
                     /locus_tag="VN084_02575"
     CDS             complement(1028..2038)
                     /locus_tag="VN084_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWT28370.1"
                     /translation="MQALDHEVLQRATEWLAQGRGVHLFTVVQTWGSAPRLPGAVLAI
                     RDDGHLVGSVSGGCIEDDLADKARHGQLPETASLIEYGVSRDEAQRFGIPCGGRLLIF
                     AEPLRDSVALMQIMHGMQQRKLIKRSVKLGTGECRLAEALPGSAPSLDGEWFHSYFGP
                     QWRLLIIGANQLGTVLASMAQALDFDVLICDPRETMRAEWHVEGATWCHGMPDDVVLE
                     LQPDAHTAIVAVTHDPKLDDMALLEALKCDAFYVGALGSRKNQEKRRERMRMFDLNES
                     EIARLRGPVGLPIGSRTPAEIAVAILAELIQVRTQQRAAAAESSDYTQAAAKTMARSC
                     AS"
BASE COUNT          407 a          669 c          704 g          418 t
ORIGIN      
        1 gaacaacgcc gcggcaagta aggtgaagcc agccagcaga taagacacaa gccgtgtctg
       61 gaaaccgacg atgatggcga tgccgcccag cacctcaagc aggatggtag gccacagcag
      121 gatagcgggc agaccgacag accccatata agctgtcgtg cccgccgggt cgttgaattt
      181 gccgatgccg gcgatcacga agatcagggc cagcaggata cggcccgcca gatgtgcgta
      241 ctttgccatg ttgtttcctc cgagtgtttt agggttgtat cgatcgaaat cgatgcatta
      301 ccataaatca attcagactg atgtaccatc cttgctccaa gcaaccgcgg cctgagcgtt
      361 cttcatgcac atcatcggta tcctgctggc aggcggccat tcgcgccgtt tcggcagcaa
      421 taaactcctg catcccatgg cggacgggca acccgttgcg ctggttgcgg cacgccactt
      481 gttggcagcc ttgccgcatt cgatcgcgat cgtccgtgac gacactccac tgggcaacct
      541 actcaaggcg gcgggcatgc aagtgatcga gtgcacgaca gcccaacagg gcatgggcga
      601 cagcctggcg gccgcagtgc gctttgccgc cgcgcagggt gaggtcgatg gcttcgtcgt
      661 tgcgctggcg gatatgcctt atatccagcc acagaccatc accgcagtgg ccgatgcatt
      721 ggctggcggc gccgccatcg cgctaccggt ccatgccggt cagcgtggtc atccggtggg
      781 gtttgccgcg aggttctcgg atgcactgca agccctggct ggggatgagg gcgcgcgtgc
      841 catcgtgcag gcaaaccggt cgatggtcct ggagatcgcc gtcgaggatg cggggatact
      901 gcgcgatatc gattatccga ccgatctaca gaccgatccg gctgcagact gatccttcag
      961 cgcgaatgcg ctatcacaaa tgaagaaacg tactcgtcca gcgttgacgg gaacgctggc
     1021 cgggaactca gctcgcgcaa ctacgcgcca tggtcttggc ggcagcctgc gtgtaatcgc
     1081 tggattctgc cgccgccgcg cgttgctggg tacgtacctg gatcagctcg gcgaggatcg
     1141 ccacggcgat ctcggctggt gtgcggctgc cgatgggtag gccgaccggg ccgcgcaggc
     1201 gggcgatctc gctttcgtta aggtcgaaca tgcgcatgcg ttcgcggcgc ttctcctggt
     1261 tcttgcgtga gccgagcgcg ccaacgtaga aggcatcgca tttcagggct tccagcagcg
     1321 ccatgtcgtc gagtttggga tcatgggtga cggcgacgat agccgtgtgg gcatccggct
     1381 gcaattccag caccacatcg tctggcatgc catggcacca ggtcgcgccc tcgacatgcc
     1441 attcggcacg catggtttcg cgcgggtcgc agatcagcac atcgaaatcc agcgcctgtg
     1501 ccatgctcgc cagtaccgtg ccaagctggt tggcgccgat gatgagcaga cgccattgcg
     1561 ggccgaaata gctatggaac cattcgccgt cgagactggg tgcgcttccg ggtagggctt
     1621 cggccagtcg gcattcacct gttcccaatt tgacagaacg cttgatcagc ttgcgttgtt
     1681 gcatgccatg catgatctgc atgagcgcca cactgtcacg caggggttcg gcgaagatca
     1741 gcaggcggcc gccgcagggg atgccgaagc gttgcgcttc atcgcggctg acgccgtact
     1801 cgatcagcga tgcggtctcg ggcaactggc catggcgcgc cttgtcggcc aggtcgtcct
     1861 cgatgcagcc gccggacacc gaaccgacca gatggccgtc gtcgcggatg gcgagtaccg
     1921 caccgggtag gcgcggggcc gagccccagg tctgcaccac ggtgaacagg tggacgccac
     1981 ggccctgcgc cagccattcg gtggcgcgct gcaagacctc gtgatcgagt gcctgcatgg
     2041 aggcctaagc cagctggctg ccgataggca agctgcggat acgcttgccg gtgagcgcga
     2101 agatggcatt ggtgacagcc ggcactactg gcggcaaggc gggttcgcca acgccgccca
     2161 tcttttcctg actcggcacg atgtgtactt cgaccttc
//
LOCUS       DATMTW010000140         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_213835, whole genome shotgun sequence.
ACCESSION   DATMTW010000140 DATMTW010000000
VERSION     DATMTW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213835"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VN084_02580"
     CDS             <1..1614
                     /locus_tag="VN084_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HWT28371.1"
                     /translation="ISGLHITMLSGLMFALVMALWRRSARLVLWLPARKAATFAAVCA
                     ALAYALVAGFSVPTQRTLYMLTIFAIALWSDREVRVGRVLALALLAVTVVDPWAVIAP
                     GFWLSFGAVAVIAYVFAGRVWRTHWLREAIISQWAVTLGLLPLLLFMFQQMSVISPVA
                     NAFAIPVVSLAVVPLTLLGSLLPIEWPLQLAHAVMALCMTALEGLAGWSASTWQQQAP
                     SALATLLAVFGVLWILLPRGFPMRWLGWIALLPLFLPAPETLRPGEMRVTIIDVGQGL
                     SVLVRTAGHRLLYDAGPKFSAHSDSGLKTIFPYLRGEGIRRLDGFVISHDDADHSGGA
                     ASLRQLLAVSWLSSSLPAHSPLLAGVRHIPCRAGQSWRWDGVGFVILGPDDTSATGAK
                     DNNRSCVLRVESAYGSLLLPGDIEREAEAALLTHAPDKLAAEVLVVPHHGSKTSSTPA
                     FIEAVQPRVAIFTAGYLNRFGHPKTAVVERYRTRGIHTYRSDREGAILLDFAVDGIHI
                     DRWRVMRKRYWQDVTADCAECLAEKGLAR"
     gene            1652..>1876
                     /locus_tag="VN084_02585"
     CDS             1652..>1876
                     /locus_tag="VN084_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWT28372.1"
                     /translation="MWEIIVAAGWPIWPLIFASIIALAIIAERFWALRTEVVAPKALL
                     PEVQKWLSQGGITKETCARLEQHSLLGEIFA"
BASE COUNT          297 a          547 c          612 g          420 t
ORIGIN      
        1 atctccggat tgcatatcac catgctttca gggctgatgt tcgccctggt catggcgtta
       61 tggcgcagga gcgcgcgttt ggtgctgtgg ctgccggcac gcaaggcggc gacattcgcg
      121 gcggtttgcg cggcgctcgc atatgccttg gtggcgggtt tttcggtgcc gacgcaacgc
      181 acgctctata tgttgaccat tttcgccatc gctctctggt cagatcggga ggtacgcgtc
      241 ggtcgggtgt tggcgctggc gctgttggca gtgactgtgg tcgatccgtg ggcggtcatc
      301 gctcccggtt tctggttgtc gttcggggcc gttgccgtca tcgcatatgt ctttgccggc
      361 cgtgtatggc gcacgcactg gctgcgcgaa gcgatcatca gccagtgggc ggtgacgctg
      421 ggtttgctgc ccttgctgct cttcatgttc cagcagatgt cagtgatctc gccagtcgcg
      481 aacgcattcg ccatcccggt cgtcagtctg gcggtggtcc cgctgacgct gctgggtagc
      541 ctgctgccga tcgagtggcc attgcaactg gcgcatgccg tgatggcgct ctgcatgacc
      601 gcgctcgaag gcttggcggg atggtcggcg agcacctggc agcaacaggc gccatccgcc
      661 ttggcaacgc tgctggctgt gttcggcgtg ctctggatat tgctgccccg aggctttccc
      721 atgcgctggt tgggatggat cgctttgctg ccgctgttct tgccggcgcc ggagactctc
      781 cggcccggcg agatgcgggt gaccatcatc gatgtcggcc agggcttgtc cgtgctggtg
      841 aggacagcag gacaccgatt gctctacgat gccgggccga agttctccgc acacagcgac
      901 agcggcctca agaccatatt cccttatctg cgcggcgaag gcatccgtcg gctcgatggc
      961 ttcgtcatca gccatgacga tgccgatcac agcggaggcg cggcatcgct gcgtcagctc
     1021 ctggcagtca gttggctcag cagcagcctg ccggcacatt cgccactgct cgccggagtc
     1081 aggcatatcc cctgcagggc ggggcaatcc tggcgctggg atggtgtcgg cttcgtgata
     1141 ctcggaccgg atgacacatc ggccacgggc gcgaaggaca ataatcgtag ttgcgtgctc
     1201 agggtcgaga gtgcctacgg cagcctgtta ctgcctggtg acatcgagcg tgaggcggaa
     1261 gccgccttgt tgacgcatgc cccggacaag ctcgccgcag aagtgctggt cgtgccgcat
     1321 catggcagca agacctcgtc gaccccggcc ttcatcgagg ccgtacagcc ccgcgtagcg
     1381 atcttcacgg ctggttatct caatcgcttc gggcatccga agacggcggt ggtcgagcgt
     1441 taccggacca gggggatcca tacataccgt tcggatcgag agggtgccat cctgctcgat
     1501 tttgccgtgg acggaatcca tatcgatcga tggcgcgtga tgcgcaagcg ttattggcag
     1561 gatgtgaccg cggattgcgc ggagtgtctt gctgaaaaag gcctcgcacg ctaaagtaac
     1621 gccactttag atatctttag gagcagcgct cgtgtgggaa attattgtcg cggccggttg
     1681 gccgatctgg ccgctgattt ttgcctcaat catcgctttg gccatcatcg ctgagcggtt
     1741 ctgggcatta cgcaccgaag tcgtcgctcc caaggcttta ctgcctgaag tccagaaatg
     1801 gctgtcgcag ggcgggatca ccaaagagac ctgcgcgagg ctggagcagc actccctgct
     1861 tggtgagatc tttgcc
//
LOCUS       DATMTW010000141         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_549312, whole genome shotgun sequence.
ACCESSION   DATMTW010000141 DATMTW010000000
VERSION     DATMTW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549312"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VN084_02590"
     CDS             <1..216
                     /locus_tag="VN084_02590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWT28373.1"
                     /translation="SIWAENFEQQATAIRRTIDDERYRVWRLYLAGCAYSFQHDQLCI
                     YQVLCQKAGQRAELLPWSRRYIYAAAG"
     gene            complement(241..2667)
                     /locus_tag="VN084_02595"
     CDS             complement(241..2667)
                     /locus_tag="VN084_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28374.1"
                     /translation="MAVKTYAYKTKDLAIFYVNKQLLKITWPFLVIVPLLLLLSIISA
                     EALSAVRAFVNGESLWSKSQKQAINALNLYAISHDEQDYRAYLDAISVPLGDRRARLA
                     LDQPKPDLEAAHRGFSAGMNHPDDIPGLIWLYRVFSDTPLMEKPIAMWVRGDAYIAEL
                     NTAAIRLHSQIANGETDFRQLRPVLETINRINTELTPIENAFSYSLGEASRDAKHLIV
                     LLTLTITLLLLTLGIYLSYRMVNQNAAANQALRESELRLRAIVDTAMDALVEVDANGI
                     ITRWSKQAEAIFAWPKGEAVGKKLYELILPANSSKAQIEEIKSLHAQLPDAHNQRLEI
                     SALRRDGTEFPAELTLSTVSWGMEAESCIFIRDITKRKEAAERLIQLAHFDSVTNLPN
                     RVLFQDRLEQQIKKANRSSLPLALMFLDVDRFKEVNDTLGHHKGDALLRETARRLRDC
                     VRETDTVARMGGDEFTIILSDLEDLTSIERITQKILRRVAEPYDLGGELAYVSVSIGI
                     TLYPNDAQAADALLKNADQAMYLAKKNGRNRASYFTAAMQEAAQRRLMLANDLHAAIT
                     DHQFKVYYQPIVQLSDGKIIKAEALARWAHPTHGLISPVDFIPIAEETGMIVDIGNWV
                     FRTAARQAMRWRDRFDPAFQVSINKSPMQFQYAREQDASWVTQIAALKVSGASIVVEI
                     TEGMMMDLNDNTKAKLIEFRDAGIQVALDDFGTGYSSLSYLNEFDIDYIKIDRSFVKD
                     LHADTDQMVLCEAIIVMAHKLGIQVIAEGVETEAQRALLQQAGCDYAQGYLFSPPIPV
                     AAFEALLKRA"
     gene            2861..3076
                     /locus_tag="VN084_02600"
     CDS             2861..3076
                     /locus_tag="VN084_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28375.1"
                     /translation="MPSLYRCREERVQDVVIAYLNEDASLRYSLTHGGQFLPFSKQEK
                     QFMRHEKAWAMARLTIDKIMRLPDMAY"
     gene            3475..4692
                     /locus_tag="VN084_02605"
     CDS             3475..4692
                     /locus_tag="VN084_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWT28376.1"
                     /translation="MRLPNYLNAAFLQLLTFRVQIVLAYQIMAVVVGWHIYQITHDPL
                     ALGLVGLAEVIPYFSTALFAGYAVDHHSRRLFGVFAALLLLLNACILFAVSLHWLAGD
                     PTWWIYGSIAVTGVARAFIGPSYNALFALILPRDQYARAAGLGSSIFQIGLVVGPALG
                     GLLVGFASTTAAYAVAMALCLGAALALLWLKVKEPPPPPRSPVFASIGEGLRFVYHNQ
                     IVLGAQCLDMFAVLFGGAVAMLPAFVQDVFHYGPEGLGILRAAPAIGAVVTGLFLARH
                     PLNRHAGRWLLVAVAGFGLSIIGFALSVDFWVAGLLLLLSGVFDGVSVVLRTTIMQLA
                     TPDHMRGRVSAINGIFIGSSNELGAFESGLSARLLGLIPSVIFGGAMTLLVVGATARL
                     APKLRRLELSQLH"
     gene            4707..5102
                     /locus_tag="VN084_02610"
     CDS             4707..5102
                     /locus_tag="VN084_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HWT28377.1"
                     /translation="MRDLDDLFQALHRSRFRRGFRLRGPDLAYAHDKGLPVVLDHAAQ
                     FIAQRLAPAHIPNDGKQTPFRGHPVFVAQHATATCCRGCLEKWHHIPADKALDVQETA
                     YVLRVLARWLSQQAGPNENRPGKTPDLFS"
BASE COUNT          922 a         1575 c         1575 g         1138 t
ORIGIN      
        1 tcgatctggg cggagaattt cgaacagcag gcgaccgcga tccgccgcac catcgacgac
       61 gagcgttacc gggtgtggcg cttgtacctg gccggctgtg cctactcgtt ccagcacgat
      121 cagctatgca tctatcaggt cttgtgccag aaggcggggc agcgggccga gctgctgccg
      181 tggtcgcgcc gctatatcta cgccgcagca ggctaaagca ggttgcaacg cctggcagcg
      241 tcaggcgcgc ttgagcagag cctcgaaagc cgcgaccggg atcggcggtg agaacaggta
      301 gccctgagca tagtcacagc cggcttgctg gagcaatgcg cgctgcgctt ccgtttccac
      361 cccttccgcg atgacctgga taccgagctt gtgggccatg acgatgatgg cttcacacaa
      421 caccatctgg tcagtatcgg catgcaggtc cttgacgaag ctgcggtcga tcttgatgta
      481 atcgatatcg aactcgttga gataggagag cgaggagtag cccgtgccga agtcgtccag
      541 cgccacctgg atgcctgcgt cgcggaactc gatgagtttg gccttggtat tgtcgttaag
      601 gtccatcatc atgccttcgg tgatctcgac gacgatgctc gcgcccgaca ctttgagcgc
      661 cgcgatctgg gtcacccagg aggcgtcttg ctcgcgtgcg tactggaact gcatcggcga
      721 tttgttgatg ctcacctgga aagccggatc gaatctgtcg cgccaacgca tggcctgcct
      781 cgctgccgtg cggaacaccc agttaccgat atcgacgatc atgccggtct cttccgcgat
      841 cgggatgaaa tcgacagggc tgatcaggcc atgtgtcggg tgagcccacc gggctagcgc
      901 ttcggccttg atgatcttgc cgtcgctcaa ctgcacgatg ggctggtaat agaccttgaa
      961 ctggtgatcg gtgatggccg catgcagatc gttggccagc atcagacgtc gctgagcggc
     1021 ttcctgcatg gcggcggtga aataactcgc gcggttgcgt ccgttcttct tggcgaggta
     1081 catcgcctgg tcggcgttct tgagcagggc atcggccgct tgcgcgtcgt tcggatacag
     1141 cgtgatgccg atgctgaccg agacataggc gagctcgcca ccgagatcgt atggctcggc
     1201 cacccggcgc aggatcttct gggtgatgcg ttcgatgctg gtcaggtctt ccagatcgct
     1261 gaggatgatg gtgaattcgt cgccgcccat gcgcgccacg gtatcggtct cacggacgca
     1321 atcgcgcagc cgccgtgccg tctcgcgcaa cagggcatcg cccttgtgat ggccaagggt
     1381 gtcgttgacc tccttgaaac gatcgacgtc gaggaacatg agcgccaatg gcaacgagga
     1441 acggttggcc ttcttgatct gctgctcgag tcgatcctgg aacaggacgc ggttgggcag
     1501 gttggtgacg ctatcgaagt gcgccagctg gatcagcctt tcggcggctt ccttacgctt
     1561 ggtgatgtcg cggatgaaga tgcatgactc ggcttccatg ccccagctca cggtggacaa
     1621 ggtcagctct gccggaaact cggtgccatc gcggcgcaag gccgaaatct cgagccgctg
     1681 gttgtgcgca tcgggcaact gggcgtgcag gctcttgatc tcttcgatct gcgccttgct
     1741 cgaattggca ggcaggatca gttcatagag ctttttaccg accgcttcgc ccttgggcca
     1801 ggcgaagatg gcttcggcct gcttgctcca tcgcgtgatg ataccgttgg catcgacttc
     1861 caccagcgca tccatcgccg tatcgacgat ggcgcgcaag cgcagctcgc tctcgcgcag
     1921 cgcctgattg gcggccgcgt tctggttcac catgcggtaa gagagataga taccgagcgt
     1981 gagcagcagc agggtgatgg tcaaggtcag cagcacgatc aggtgcttgg catcgcgtga
     2041 cgcttcgccc aggctgtagg agaacgcatt ctcgatcggc gtcagttcgg tattgatgcg
     2101 gttgatggtc tccagcaccg gcctcagttg ccggaaatcg gtctcgccat ttgcaatctg
     2161 gctatgcaac cggatcgctg cggtgttgag ctcggcgata taggcgtcgc ccctgaccca
     2221 catggcgata ggcttttcca tcagcggggt gtcgctgaac acacgataga gccagatcag
     2281 ccccgggata tcgtccggat ggttcatgcc tgcgctgaaa ccgcgatgag ccgcctccag
     2341 atcaggtttg ggctggtcca gcgcaagccg ggcccggcga tcgcccagcg gtacggagat
     2401 cgcgtcgaga taggcccggt aatcctgctc gtcatgcgag atggcgtaga gattgagggc
     2461 attgatcgcc tgcttctgcg atttcgacca caggctctcg ccgttgacga aggcccgcac
     2521 cgcagacagg gcttcggcgc tgatgatgct cagcaacaga agcaggggaa cgatcacgag
     2581 gaacggccaa gtgatcttca gtagctgttt gttgacgtaa aaaatcgcaa gatcctttgt
     2641 cttgtatgcg tatgttttta ctgccactgc aggccgccgc tctccccaga accatgcggc
     2701 catctcttaa tgtagcgtga gccattatgc ttttcaacgc tcatgtcatc cgagcgccca
     2761 tagtggtcac caccggttgg tgagtcatca catcaggcgt attgttcttt gacgccaaag
     2821 cttgatacga taggctggat tcatctatcg gggagctgta atgccgtcgt tatatcgatg
     2881 ccgggaagaa cgggtacagg atgtcgtgat cgcttacctg aacgaggatg cctcgctcag
     2941 gtattcgctg acgcacggcg gccagttcct gccgttcagc aagcaggaga aacagttcat
     3001 gcggcacgaa aaggcctggg cgatggcacg gctgaccatc gacaagatca tgcggctacc
     3061 ggatatggcg tattagcgca ttcgggctcg cccctgccac gaggagcatg acgcgtaggg
     3121 cacatggagg gtggagcgcc cagcaagcag ggcgggaagt gtgggcagcg cgtggtaaac
     3181 agcaagtggg gaatagtaag tgttagtgcg cctaatccat ctcgacagaa ccccactagg
     3241 tcaagggcga ctcatcggcc cgtcatatcg ggcttactgc cttattcact gcactaaccg
     3301 catgattata cctgcactgg cgccgcttca gctctggtat cctatcggtc tttcatctta
     3361 gcggtctttt attctagtgg gcttgtcgcg ccaccctgga tcctgatcgc gctgtcttga
     3421 accctgtaac ttgaaccctg taagtcatca acccccggaa gccctctggc acccatgcgt
     3481 ttacccaact acctgaatgc cgcattcctg cagctcctga cctttcgcgt gcagatcgtc
     3541 ctcgcctacc agatcatggc cgtggtcgtt ggctggcaca tctaccagat cacgcatgac
     3601 ccgctggccc tcggcctggt ggggctcgcc gaagtcatcc cctacttcag cacggccctg
     3661 ttcgccggct acgcggtgga tcaccactcg cgccggctat tcggcgtgtt cgcggcattg
     3721 ctgctgttgc tcaatgcctg catcctgttc gccgtatcgc tacactggct tgcaggcgac
     3781 ccgacctggt ggatctacgg atcgatcgcc gtcaccgggg tcgcgcgtgc tttcatcggc
     3841 cccagctata acgcgctgtt cgccttgatc ctgccacgcg accaatacgc aagggccgct
     3901 ggtctcggca gctccatctt ccagatcggc ctggtcgtcg gcccggcgct gggcgggctg
     3961 ctggtcggct tcgccagtac cacggcggct tacgccgtcg ccatggccct gtgcctcggc
     4021 gccgcactgg cattgctctg gctgaaggta aaggaaccgc cgccgccccc tcgctcacca
     4081 gtgttcgcca gcatcggcga gggcctgcgt ttcgtctatc acaaccagat cgtgctcggc
     4141 gcccaatgtc tcgacatgtt cgccgtgttg ttcggtggtg ctgtggccat gctgccggcg
     4201 ttcgtgcagg acgtcttcca ttacggcccg gaagggttgg ggatcctgcg tgccgcgcca
     4261 gcgatcggcg ctgtcgtcac tggcctcttt ctcgcccgcc atccgctcaa tcgccacgcc
     4321 gggcgctggt tgctggtcgc cgttgccggt ttcgggcttt ccatcatcgg cttcgcattg
     4381 agcgtcgatt tctgggttgc cggtctgctg ctgttgctct ctggcgtctt tgacggcgta
     4441 tcggtagtgc tgcgtaccac catcatgcag ctggccacgc ccgaccacat gcgcggacgc
     4501 gtctcggcaa tcaacggcat cttcatcggt tcgtcgaacg agcttggcgc cttcgaatcc
     4561 ggcctctcgg ccagactgct gggcctgatc ccatcggtga tcttcggcgg ggccatgacg
     4621 ctactggtgg tcggtgccac ggccaggctc gcgcccaaac tgcgacggct ggaattgagc
     4681 cagttgcatt agccgcgcca aacgtcatgc gggatcttga tgacctcttc caggccttgc
     4741 acagatcgcg cttccgtcgc ggtttccgct tgcgcggccc ggatctggcc tatgcgcatg
     4801 acaagggtct gcccgtcgtg ctggaccacg cagcgcaatt catcgcccag cgccttgcgc
     4861 cggcgcatat ccccaacgac ggcaagcaga ccccgttccg cggacacccg gtgttcgtcg
     4921 cccaacatgc cactgcgaca tgctgtcgcg gctgcctgga gaaatggcac catatcccgg
     4981 cggacaaagc actcgatgtg caggaaaccg cctatgtgct acgggtactg gcccggtggt
     5041 tgtcacagca agcaggccca aacgaaaaca ggcctggcaa aacgccagac ctgttctctt
     5101 gatgacctgc caacgcggcg agtttatctg gtgatgggtt tgtagcgaat gcgcttaggt
     5161 tttgcgcctt tgtagccgaa cttaccacag gctgcttcgt ggtccagttc
//
LOCUS       DATMTW010000142         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_515980, whole genome shotgun sequence.
ACCESSION   DATMTW010000142 DATMTW010000000
VERSION     DATMTW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515980"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VN084_02615"
     CDS             complement(<1..697)
                     /locus_tag="VN084_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWT28378.1"
                     /translation="MNEAHAAPAACISTYRTQAHTAGLYLTHTVHNLLLTVFNFGLGA
                     VLGAAGGLLGIGGGLIAIPILSYLYGMDQHLAQGTALVMIVPNVLMGLLRYHQKHRID
                     LRSAAAIVLFATASSYVAARLAAGISSSDLHTLFAVFLVVLALYFGWPKRSSASDDDV
                     HASPARPIPQAALPLLGIASGGMSGVFTVGGSLVVVPALVSLFGMQQTRAQGLALTLV
                     VPGALVALVAYAQE"
     gene            complement(694..2028)
                     /locus_tag="VN084_02620"
     CDS             complement(694..2028)
                     /locus_tag="VN084_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HWT28379.1"
                     /translation="MDAATKAASAHAPVVRLNAWQALMRREIGPLPLPVYLAIAGITI
                     AAALNGKLPNDMIGGLSVLMLMGFLLGDIGNRIPVLKQIGGAAILCLFVPSALIGYGL
                     LDQGMQKALTTTMKTANVQYLYIAGLVAGSILGMRHRVLVQGFMRMFVPLLAGTAAAI
                     TAGVLVGLLFGYDPMHTFFFIVIPILGGGIGEGILPLSIGYAEILGKSQAELVAMMVP
                     AALVGNVVAIVASGILRWLGDRKPELNGHGMLVKSGDDAELLNDTQERQPLDLSLMGA
                     GLLIACAFFILGALLARVTGIPGPILMIVSAALVKVFKVLPARMELGAYQMYQFFSKN
                     LTFAILVGLGALFVSWKDLVHAFTPGYIAICAASVGAMVTCGFFVGKWLNMYPVESGI
                     VTACHSGLGGTGDVAILTASNRMGLMPFAQISTRIGGAAMIVAATLLLKLFH"
     gene            complement(2109..>2828)
                     /gene="leuD"
                     /locus_tag="VN084_02625"
     CDS             complement(2109..>2828)
                     /gene="leuD"
                     /locus_tag="VN084_02625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWT28380.1"
                     /translation="PPRSPAVSPTYDNLEHPMRDMNPDIARIEGIAAPLPTPNLDTDQ
                     IMPKQLLRGIDKSGLDRGLLYDMRFDGEHRPRPDFVLNRPEYGNTAILIGGSNFGCGS
                     SREHAVWGLQQYGIRAVIASSYGEIFYSNAMNNRLLLVMLPEDAVQKIMADVSDPATS
                     HVTIDVERMTVRSHSVETTFSLSGRHRHMFLEGLDMIGATLTLQEQIQAFQQGHWAAR
                     PWLKDVAHTTKARLASSKTQQ"
BASE COUNT          583 a          874 c          890 g          481 t
ORIGIN      
        1 cctcctgcgc gtaagcgaca agagcaacca gcgcacccgg cacgacgagc gtgagcgcca
       61 gaccttgcgc acgggtctgc tgcatgccga acagactaac gagagccggc accacaacaa
      121 gtgaaccgcc gacggtgaat acgccggaca tgccaccact cgcaatgccg agcagcggaa
      181 gggccgcctg cggaattggc cgcgcgggcg atgcgtgtac atcatcgtcc gaggctgaag
      241 aacgcttggg ccatccgaaa tacagcgcga ggacgaccag gaacaccgcg aacaaagtat
      301 gcaagtcact agagctgatg cctgcagcga ggcgcgccgc cacgtagctg ctcgcggtcg
      361 cgaacaagac tatcgccgct gccgaacgca gatcgatgcg atgcttctgg tggtaacgca
      421 acaggcccat caggacgttc ggcacaatca tgactagtgc cgtgccctgt gccagatgct
      481 gatccatgcc gtacagatag ctaaggatcg ggatcgcgat cagaccacca ccgataccca
      541 gcaggccgcc ggccgcgccg agcaccgcgc caaggccaaa attaaataca gtgagaagca
      601 ggttgtgcac agtatgggtc aggtaaagcc cggcagtatg agcttgggta cgataagtgg
      661 aaatgcaggc tgccggagca gcgtgcgcct cgttcaatgg aacagtttca gaagcagggt
      721 agcggccacg atcatcgcgg ccccaccgat gcgggtcgag atctgcgcga acggcatcag
      781 gcccatgcgg ttcgacgccg tcaggatcgc cacgtcgccg gtaccgccca ggccgctgtg
      841 gcaggccgtc acgatacccg actcgaccgg gtacatgttc agccatttgc cgacgaagaa
      901 gccgcacgtg accatggcac ctacgctggc ggcacagatc gcgatgtagc ccggcgtgaa
      961 cgcatggacc aagtccttcc acgatacgaa cagggcaccg agaccgacga ggatcgcgaa
     1021 cgtcaggttc ttcgagaaga actggtacat ctgataggca ccgagctcca tcctggccgg
     1081 caggaccttg aagaccttga ccagtgcggc gctgacgatc atcaggatcg ggccggggat
     1141 acccgtcacc ctggcgagca gcgcacccag gatgaagaag gcgcatgcga tgagcaggcc
     1201 ggcacccatc aggctcaggt cgagcggctg gcgctcctgg gtgtcgttca gtagttcggc
     1261 gtcgtcgccc gatttgacca gcatgccatg gccgttcagc tccggtttgc ggtcgccgag
     1321 ccaacgcagg atgccgctgg ccacgatcgc gaccacgtta ccgaccaggg cagcgggtac
     1381 catcatcgcg accagttccg cctgcgactt gccgaggatt tccgcgtacc cgatcgacag
     1441 cggcaagatg ccttcgccga tgcccccacc gaggatcggg atgacgatga agaagaacgt
     1501 gtgcatcgga tcgtagccga acagcagacc gaccagcacg ccggccgtga tcgccgccgc
     1561 ggtaccggcc agcagcggta cgaacatgcg catgaagccc tgcaccagca cgcgatggcg
     1621 catccccagg atgctgccgg cgaccaggcc ggcgatgtac aggtattgca cattggccgt
     1681 cttcatcgtg gtcgtgagag ccttttgcat gccctggtcc agcagcccgt aaccgatcag
     1741 ggccgagggc acgaacaggc acaagatggc ggcgccgccg atctgcttca gcaccgggat
     1801 gcggttgccg atatcgccaa gtagaaagcc catcagcatc aggacggaca ggccgccaat
     1861 catgtcgttg gggagtttgc cgttcagcgc cgcggcaatc gtgatgcccg cgatcgcaag
     1921 gtagaccggt aatggtagag ggccgatctc gcgccgcatg agagcctgcc acgcgttcag
     1981 acgtacaaca ggcgcgtgcg cgcttgctgc cttggttgct gcatccatat tgtctccttg
     2041 tgggacactg gtcccttatg aatgggtaac cgcccggaca agtagaatcc ccgggcgatt
     2101 tcgccgtgtt actgttgcgt cttcgacgac gcaaggcgcg ccttggtcgt gtgagcaaca
     2161 tccttcaacc agggccgggc ggcccagtgt ccttgctgga aagcctgaat ctgttcctgc
     2221 aacgtcaggg tggcgccgat catgtccagc ccctcgagga acatgtgacg atgacgcccc
     2281 gacaggctga acgtcgtctc gacgctgtgg ctcctcaccg tcatgcgctc gacgtcgatc
     2341 gtgacatggc tggttgccgg atcggacacg tcggccatga ttttctggac cgcgtcctcg
     2401 ggcagcatca ccagcagcag gcggttgttc atcgcattgc tgtagaagat ttcgccatag
     2461 ctggacgcga tcacggcacg aatgccgtac tgctgcaggc cccagacggc atgttcgcga
     2521 ctcgatccgc agccgaaatt gctgccgccg atcaggatgg cggtattgcc gtattccgga
     2581 cggttcagga cgaagtccgg acgcgggcga tgctcgccat cgaagcgcat gtcgtacagc
     2641 agaccgcggt ccaggcccga tttgtcgatc ccgcgcagga gctgcttggg catgatctgg
     2701 tcggtgtcca ggttcggcgt cggcagtggc gccgcgatgc cttcgatacg ggctatgtcg
     2761 gggttcatgt cacgcatggg atgctccaaa ttgtcgtacg tcggtgatac ggccggtgac
     2821 cgcggcgg
//
LOCUS       DATMTW010000143         9850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_650366, whole genome shotgun sequence.
ACCESSION   DATMTW010000143 DATMTW010000000
VERSION     DATMTW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650366"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VN084_02630"
     CDS             <1..434
                     /locus_tag="VN084_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWT28381.1"
                     /translation="RLGAIICDASAHDEQAMAEYGMRLGTAFQLIDDVLDLSGNSGEI
                     GKNLGDDLAEGKPTLPLLYAMRNGDSRQRETIRRAIEQGGLEELPTVIQAVRDTGALD
                     HVRRLARAEAERGCAAIAHLPAGDYHTALIDLANFAVERSF"
     gene            complement(455..781)
                     /locus_tag="VN084_02635"
     CDS             complement(455..781)
                     /locus_tag="VN084_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28382.1"
                     /translation="MTDVSTQAQDAWETPVVCRVEVDLGAWLEQLTGKANWELFSEDE
                     DEAYMSFAMRQGRQHAEVTLYHNGYAVVDVDGQALFDGSLTTGTSDCAHLSYYYAETG
                     EPVMLN"
     gene            923..1783
                     /locus_tag="VN084_02640"
     CDS             923..1783
                     /locus_tag="VN084_02640"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWT28383.1"
                     /translation="MTDFFTMLSEHATMFITFSVVFGLMVGSFLNVVIHRLPQMMERE
                     WQAHCQEVSGQEVAPLPKYNLVVPRSACPGCGLPISAWQNIPLLSYVMLGGKCAGCKQ
                     AISPRYPLIEALTGILAGLISWKFGYSSMAVAAWLFAFALVALTFIDLDTQLLPDDIT
                     LPLLWLGLLYNLDGGFTDIRSAIIGAAAGYLLLWSVYWLFKLVTGKEGMGYGDFKLLA
                     AIGAWFGWQLLPAVILLSSLLGSIIGIGLVILAKRGRDIPMPFGPYLALGGIAALFFG
                     PQLSQFYLGG"
     gene            1789..2397
                     /gene="coaE"
                     /locus_tag="VN084_02645"
     CDS             1789..2397
                     /gene="coaE"
                     /locus_tag="VN084_02645"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480447.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWT28384.1"
                     /translation="MYVVALTGGIGAGKSEVAKIFASLGVPIVDVDVISRRLTGPGQP
                     IMDKIAEAFGRDFLTEDGALNRPAMRERIFANDEDRKKLEAILHPVIHSHALRELDQN
                     REAPYQILAIPLFFETNRYSGLVKRILLVDCDETLQISRTMNRNGMTEHTVRAIIAAQ
                     ASRSYRRALANDIIENDSSIEALAEKVKALHEKYMQACLLSD"
     gene            2459..3217
                     /gene="zapD"
                     /locus_tag="VN084_02650"
     CDS             2459..3217
                     /gene="zapD"
                     /locus_tag="VN084_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HWT28385.1"
                     /translation="MPSYDYPFNERIRTLLRLEDLFVKVLHNIEQPHEFNHHAALIAL
                     FQMLDVIDRAELKTDLLQELDRQKIVMNGLRGNPAIAEQVLNDVISQIETTALALRGN
                     TVKLGQSLRDNEWLMSIKQRAGIPGGVCEFDLPSYHYWLGLDAERRRSDLERWLEPLM
                     PVHEALRIILHILRGSGATSKMVAEHGSYQQMLGGAKPAQMMRIELDETLPCFPEVSA
                     NKYAINIRFNSLDAAQKPRQCDMDVHFKLTLCNL"
     gene            3214..3402
                     /locus_tag="VN084_02655"
     CDS             3214..3402
                     /locus_tag="VN084_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HWT28386.1"
                     /translation="MTSPKKKTLVTCPQCGNVSEYSRDNPFRPFCSERCKLIDLGQWA
                     SENYRIPEPLKPEDLPEA"
     gene            3485..3922
                     /locus_tag="VN084_02660"
     CDS             3485..3922
                     /locus_tag="VN084_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWT28387.1"
                     /translation="MTRSALIASLLALTSLPALAGDDVEITNAWTRATAPGQQVGAAY
                     MTLTSKTDMTLESVESPVAGSVEIHSMTMKDGVMRMRKLETLPLKANAPEHLAPGGFH
                     LMLFDLKKPLKIGETVDFTLHLKDASGHDSTQHLVSPIKRSAD"
     gene            complement(3939..4370)
                     /locus_tag="VN084_02665"
     CDS             complement(3939..4370)
                     /locus_tag="VN084_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28388.1"
                     /translation="MNRVKLAGMAALLLLAALLAAIGVTRYLNGAASPVVQVIGCKDI
                     VRGCNGDGLRVRMDRAPEIMRPFKLEVEAERADTIEASFQMAGMEMGFNRYRLQRQDG
                     DVWQAEVTLPICVRGRKDWLLLLEVHEGQAQKQVAVPFSTR"
     gene            complement(4367..5329)
                     /locus_tag="VN084_02670"
     CDS             complement(4367..5329)
                     /locus_tag="VN084_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HWT28389.1"
                     /translation="MTSTTTGTVATEVVHAAVAVIQRTDGCVLLGQRPQGKPWAGWWE
                     FPGGKIEAGEAAIDALGRELDEELGIEVIEATPWLTRVFAYPERTVQLHFFMVRQWRG
                     EPHGREGQQLAWQQPGDLSVGPMLPANLPIVEALQLPPVYAISNLAELGEARFIAALE
                     KALQQGLRLMQWREPDLPPAHREALLQQVLELANRYGARVLVNADIALARAAGAHGVH
                     LPARALMAMQQKPAGLCCAASCHTVEELAQAARLEIDFVVLSPVLPTPSHPGAATLGW
                     TAFAEMVADYPIPVYALGGMRDEHLHTAWLHGAHGIAMQRGVWT"
     gene            complement(5339..6208)
                     /locus_tag="VN084_02675"
     CDS             complement(5339..6208)
                     /locus_tag="VN084_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28390.1"
                     /translation="MADTDTVLQRAEALLSRLEGLMQHTPAEPDWSALAWRWQHNNGR
                     GYLEPVAFPHQIKLDDIHNVDEQKVAIVQNTEQFVKGYTANNVLLTGARGTGKSSLVK
                     ALLSQFAKHGLRIVEVDKQDLTDLPQITALLRGREERFIVFCDDLSFESGEPGYKALK
                     VVLDGSVATTSDNIVVYATSNRRHLMPEYMAENLETKYVGDEIRPAEAVEEKVSLSDR
                     FGIWLSFYPFAQDEYLVIAEQWLRHYGIKKFDDQARGAALQWALTRGSRSGRSAHQFA
                     RDFAGRAALAAKK"
     gene            complement(6211..7440)
                     /gene="argJ"
                     /locus_tag="VN084_02680"
     CDS             complement(6211..7440)
                     /gene="argJ"
                     /locus_tag="VN084_02680"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWT28391.1"
                     /translation="MPVNLSAPQAQDLLPVKGVQLGIAKAGIRKPDRKDMLVMKLAEG
                     TRVAGVFTKNRFCAAPVTLCKSHLAQDTGIRALVVNTGNANAGTGESGLQDARATCAE
                     LAALLACRPEQVLPFSTGVILEPLPMGRLLAGLPGCIADLKEDNWRSAADAIMTTDIV
                     AKGVSRQVTVGGKTITITGISKGSGMIHRNMATMLGYIATDAAVSQAALEQIIQFAVN
                     RSFNCITVDGDTSTNDSLILMATGSAGNPEIASEQDAGYLVLRDALTEVATLLAQAIV
                     RDGEGATKFMTIRVEAGRDEEECRKVAYAIAHSPLIKTAFFASDPNLGRILAAIGYAG
                     IDDLDVNQLKLYLGDVLVAEHGGRATSYEEAQGAAVMKESDITVRVTLNRGTAEATVW
                     TCDFSYDYVKINADYRS"
     gene            7581..>9850
                     /locus_tag="VN084_02685"
     CDS             7581..>9850
                     /locus_tag="VN084_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWT28392.1"
                     /translation="MNNPAVVQEKEAASLTEMGNKLAFTKKLQAVTNKIHATRNIDEI
                     ILEVSQDIRALFEAERLTLYVTNDDESAIVSKVKTGLNEFKELKLAINPQSIAGYVAF
                     NKSILNIADVYDDAELERISPEIHFLKEVDKKTGFRTKQMLIFPILSQEDSALMGVVQ
                     VINTMSGLPFPQMAEEGAPELAKTLAIALSQRQAPIGVVKTKFDTLVSEGIISSTELE
                     LAQRSARKKGKDLEEVLIEEFQVKPTDIGKSLATFFGVALEPFKVDRVKPMDLLKNLK
                     PDYVMSNGWLPIDETKEEGLVILTTDPEKVRASRIVSNIFPRHAKITYRVCLHRDFSN
                     TVNQFYGVSGLSGDIDDILSTMGEDEEDSGGIGAEDISAAADNELVKLVNKIIIDAYN
                     QGASDIHIEPYPDKGKTEVRFRKDGTLVNYIEIPPSYRNAIVARIKIMCDLDIAERRK
                     PQDGKIKFKKFGPLDIELRVATIPTAGGIEDIVMRILAAGKPIPLDKLGLSEHNNKKL
                     KELVAKPYGLFFVCGPTGSGKTTTLHSALGFINTPETKIWTAEDPVEITQKGLRQVQV
                     NKKAGLDFAMVMKAFLRADPDVIMVGEMRDKETVSTGIEASLTGHLVFATLHTNSAPE
                     SIVRLLDMGMDPFNFADAMLGILAQRLGKRLCECKKAYTPDANEIKGMLNEYCEELKN
                     TSSWKKDPEAAYKAVYQNWVKSFGNDKGQLTLYKAIGCDKCNSSGYKGRVGLHELLEG
                     TDAIKKNIQEHARVAD"
BASE COUNT         2056 a         3074 c         2862 g         1858 t
ORIGIN      
        1 cccggcttgg cgccatcatc tgcgatgctt cggcgcacga cgagcaggcc atggcggaat
       61 acggcatgcg ccttggtaca gcgttccagc tgatcgacga tgtgctcgac ctcagtggta
      121 atagcggtga gatcggcaag aacctcggtg acgacctcgc ggaaggcaag ccgacactgc
      181 cgctactcta cgccatgcgc aacggcgact cccggcaacg cgaaaccatc cggcgcgcca
      241 tcgagcaggg cgggctggaa gaattgccga ccgtgatcca ggcagtcagg gataccgggg
      301 cgctcgacca tgtgcgccga ttggcgcgcg cagaggccga acgtggctgc gccgcgatcg
      361 cacacctacc ggcaggggat taccacacgg cgctcatcga ccttgcgaat ttcgctgtcg
      421 agcgttcatt ctgaagtgac cggttgccag ccgctcagtt gagcatcact ggttcgccgg
      481 tctcggcgta gtaataactc agatgggcgc aatcgctggt gcctgtcgtc agcgaaccgt
      541 cgaacaaagc ctggccatct acatccacca cggcatagcc attgtgatac agggtgactt
      601 ccgcgtgctg gcgtccctga cgcatggcga agctcatgta agcctcgtcc tcatcctcgg
      661 agaagagttc ccaattggcc ttccccgtca attgctccag ccacgcgcct agatccacct
      721 ctacgcggca aacgactggg gtttcccatg catcttgcgc ttgcgtgctg acgtcggtca
      781 tttgcgatcc ttttgaaagt gattgtcgat tgctatctag tttgtgacat atcgcgtaat
      841 ttcaaggccc ggtgcggccc cggaaggttg ccttgcatgt ataacggtgg gatgatgcag
      901 caagtttact ggacggcgcc ttatgactga ttttttcacc atgctgagcg agcatgcgac
      961 gatgttcatc accttttccg ttgtgttcgg tctcatggtc ggcagttttc tcaatgtggt
     1021 catccaccgc ctgccgcaga tgatggagcg ggaatggcaa gcccattgcc aggaagtcag
     1081 cggccaggag gtcgcgccgc tgccgaaata caatctggtg gtgccgcgtt cagcctgtcc
     1141 cggttgtggg ctacccatca gcgcgtggca gaacattccg ctcctcagtt acgtcatgct
     1201 gggtggcaag tgcgccggct gcaaacaggc catcagtccg cgctatccgc tgatcgaagc
     1261 cctgacgggc atcctggccg gccttatcag ctggaagttc ggctactcgt cgatggctgt
     1321 tgcggcttgg ctgttcgcgt tcgccctcgt cgcactcacc ttcatcgacc tggacacgca
     1381 gctgctgccg gacgacatca cactgccact gttatggctg ggactgcttt ataacctgga
     1441 tggcggcttc accgacatcc gctcggcgat cattggcgcc gctgcaggct atctgctgct
     1501 gtggtcggtc tactggctgt tcaagctggt caccggtaag gagggcatgg gttacggcga
     1561 tttcaagcta cttgcggcga tcggtgcgtg gttcggctgg caattgctgc cggccgtgat
     1621 cctgttgtct tcgctgctcg gctcgatcat cggcatcggc ctcgttattc tcgccaagcg
     1681 tgggcgcgac atccccatgc cgtttggtcc ttatcttgct ttaggtggta tcgcggcctt
     1741 gttcttcgga ccgcagttgt cacaatttta tctgggaggt tgatcatcat gtatgtcgtg
     1801 gcactgaccg gcggtatcgg cgctggcaag agcgaagtcg ccaagatatt cgcatccctc
     1861 ggagtgccga tcgtggatgt cgacgtcatc tcgcgcagac tgaccggccc cggccaaccg
     1921 atcatggaca agatcgcgga agccttcggc cgcgatttcc tgaccgagga cggtgcattg
     1981 aaccgcccgg cgatgcgtga acgcatcttt gccaacgacg aggatcgcaa gaagctggaa
     2041 gccatcctgc acccggtgat tcacagccac gcattgcgcg agctcgacca gaaccgcgag
     2101 gcgccgtacc agatcctcgc cattccatta tttttcgaga ccaatcgtta ctctggcctg
     2161 gtcaagcgca tcctgctcgt ggattgcgac gaaaccctgc agatcagccg caccatgaac
     2221 cgtaacggca tgaccgagca taccgtgcgt gccatcatcg cagcccaggc ttcgcgcagt
     2281 tatcgccgtg cgctggcgaa cgacatcatt gaaaatgaca gctcgatcga agcccttgca
     2341 gaaaaggtca aggcgctgca tgaaaaatat atgcaagctt gcttacttag cgattaaatt
     2401 tcataattca ccgcaatatt ttcagcgcta accaagatta gccggtaaaa aaaatcccgt
     2461 gcccagctac gactatccct tcaacgaacg tatccgcaca ctgctgcggc tcgaggacct
     2521 gttcgtcaag gttttgcaca acatcgagca gccgcacgaa ttcaatcacc atgccgcgct
     2581 gatcgccttg ttccagatgc tcgacgtcat cgaccgcgcc gagctcaaga ccgatctgct
     2641 gcaggagctt gatcgccaga agattgtgat gaacggtctg cgcggcaacc cggcgatcgc
     2701 cgaacaggtg ctgaacgacg tcatcagcca gatcgagacc acggcgctgg ccttgcgcgg
     2761 caacaccgtc aagctcggcc agtcgctgcg tgacaacgag tggctgatga gcatcaagca
     2821 gcgcgccggt atcccggggg gcgtctgcga attcgaccta ccttcctatc actattggct
     2881 gggcctggat gcggaacgtc gtcgcagcga cctggaaaga tggctggaac cgctgatgcc
     2941 ggtgcatgaa gcactgcgta tcatcctgca catcctgcgc gggagcggcg cgacctcgaa
     3001 gatggtagcc gaacacggca gctaccagca gatgcttggc ggtgccaagc cggcgcagat
     3061 gatgcgcatc gaactggatg agacgctacc ctgctttcct gaagtcagcg ccaacaagta
     3121 cgccatcaat atccgtttca atagtctgga cgcggcgcag aaaccacgac aatgcgacat
     3181 ggacgtccac ttcaagctga cgctgtgcaa cctgtgacct caccgaaaaa gaagacgctg
     3241 gtcacgtgcc cgcaatgcgg taacgtcagc gaatattccc gcgacaaccc gttccggccg
     3301 ttctgcagcg aacgctgcaa gctcatagac ctcgggcagt gggcgagcga gaactatcgc
     3361 atcccggaac cgctcaaacc ggaagattta ccggaagcgt gaacccttca tgccgggcgc
     3421 tcgcccaacg ctagagccca tgcattcgat atcaccaacg cgtcctcaac caaaggatag
     3481 tcgcatgaca cgaagcgccc tgatcgcaag cctgctcgca ttgaccagcc tcccggcgtt
     3541 ggccggcgac gacgtcgaga tcaccaacgc ctggacgcga gccacagcgc cggggcagca
     3601 ggtcggcgcg gcctacatga cactgaccag caagaccgac atgaccctgg aaagtgtcga
     3661 aagcccggtg gccggtagcg tggagatcca cagcatgacc atgaaagacg gtgtcatgcg
     3721 catgcgcaaa ctggaaacgt taccgctgaa agccaacgcg ccagaacacc tcgcgcccgg
     3781 tgggttccat ttgatgctgt tcgacctgaa gaagccgctc aagataggcg agacggtgga
     3841 cttcaccctg catctcaagg atgccagcgg tcacgacagc acgcagcatc tcgtatcgcc
     3901 gatcaagcgt agcgccgact gaggctcgca gcgcagacct agcgcgtgct gaatgggacg
     3961 gccacctgct tctgtgcctg tccttcatgc acctccagca acaacagcca atccttgcgg
     4021 ccgcgtacac agatcggcaa ggtcacctca gcctgccaga catcgccatc ctgtctctgc
     4081 aggcgatatc gattgaatcc catctccatg ccagccatct gaaaactggc ttcgatcgta
     4141 tccgcccgct ccgcttcaac ctccagcttg aacggccgca tgatctcggg agcccggtcc
     4201 atacgcacgc gtagcccgtc gccgttgcag ccccgcacga tatccttgca gcctatcacc
     4261 tgcacgactg gcgacgcggc accgttgaga tagcgcgtga cgccgatcgc cgccaacaag
     4321 gcggccaaca acagcaatgc cgccatcccg gcgagcttga cgcgattcat gtccacaccc
     4381 cccgctgcat ggcgatcccg tgggcgccat gcagccacgc cgtgtgcaga tgctcatcgc
     4441 gcatgccgcc cagggcatag accgggatcg ggtaatcggc gaccatctcg gcaaacgctg
     4501 tccagcccag cgtagcggct cccgggtgac tcggtgtagg caacaccggc gagagcacga
     4561 caaaatcgat ctccagcctg gccgcctgcg ccagctcttc aaccgtgtgg caggaagcgg
     4621 cacagcacag ccccgccggc ttctgctgca tcgccatcaa ggcgcgcgcc ggcaagtgca
     4681 ccccgtgggc acctgcggcg cgggctagcg cgatatcagc gttgaccagc acccgtgcgc
     4741 cgtagcggtt cgccagttcc agcacttgtt gcagcagcgc ctcgcggtgc gccggcggca
     4801 gatcgggttc acgccactgc atgaggcgca gcccctgctg caaggctttt tccagggcgg
     4861 caatgaagcg tgcttcgccc aactcggcca gattggagat ggcgtacacc ggcggcagct
     4921 gcaaggcctc gacgataggc aggttggccg gcagcatcgg gccgacgctc aaatcgcctg
     4981 gctgttgcca ggcaagctgc tggccttccc tgccgtgggg ctcaccgcgc cattggcgga
     5041 ccatgaagaa atgcagttgc acggtgcgtt cagggtaagc gaagacccgc gtcagccagg
     5101 gcgtcgcttc gatcacctcg atgccaagct cttcgtcgag ctcgcgaccg agcgcgtcga
     5161 tcgcagcctc ccccgcttcg atcttgcccc ccgggaactc ccaccagcca gcccatggct
     5221 tgccttgtgg acgctgcccg agcagcacgc agccatccgt ccgctgtatg acggccacgg
     5281 cggcgtgcac gacctcagtc gcgactgtcc cggtagttgt cgacgtcatg cgcgatgctt
     5341 atttcttggc ggccagcgcg gcacggccgg cgaagtcacg cgcgaactgg tgcgccgaac
     5401 gaccactacg cgaaccgcgc gtcagcgccc attgcagcgc ggcaccacgg gcctggtcgt
     5461 caaacttctt gataccgtaa tgcctgagcc attgttcggc gatcaccaga tactcgtcct
     5521 gcgcgaaagg gtagaacgat agccagatgc cgaaacggtc agacaaggag accttttcct
     5581 cgaccgcctc cgccgggcgg atctcgtcac cgacgtactt ggtctcaaga ttctcggcca
     5641 tgtattctgg catcaggtgt cggcggttgg aggtggcata gaccacgata ttgtccgagg
     5701 tggtcgccac cgagccgtca agcacgacct tgagcgcctt gtagccgggc tcgccggatt
     5761 cgaaggaaag atcgtcgcag aacacgatga agcgttcctc gcgaccgcgc agcagcgccg
     5821 tgatctgcgg caggtcggtc aggtcctgct tgtcgacttc gacgatgcgc aggccatgct
     5881 tggcgaactg gctgagcagg gctttcacca gcgatgactt gccggtgccg cgcgcgccag
     5941 ttagcaggac gttgttggcg gtgtagcctt tgacgaactg ctcggtgttc tgcacgatcg
     6001 ctaccttctg ttcgtccacg ttgtggatat cgtcgagctt gatctggtgc ggaaacgcca
     6061 cgggctcaag gtagccgcgg ccattattgt gctgccagcg ccaggccagc gccgaccagt
     6121 ccggttcggc cggcgtatgc tgcatcaagc cctccagacg gctgagcaag gcttccgcac
     6181 gctgtagaac ggtgtccgtg tctgccatcg ttagctcctg taatccgcgt tgatcttcac
     6241 gtagtcatac gagaaatcgc aggtccagac cgtcgcttcg gcggtaccgc gattcagcgt
     6301 cacgcgcacg gtgatatcgc tttccttcat gacagccgct ccctgagcct cctcgtaact
     6361 tgtcgcacgg ccaccatgct cggccaccag cacatcgccc agatacagct tgagctggtt
     6421 gacgtcgagg tcatcgatac cggcataacc gatagcggcc aggatacggc ccaggttagg
     6481 gtcggaagcg aagaacgcgg tcttgatcag cggtgaatgg gcaatggcat atgcgacctt
     6541 gcgacattcc tcctcatccc tgccggcctc gacccggatg gtcatgaact tggtggcccc
     6601 ctcgccgtcg cggacgatgg cctgggccaa cagcgtcgcc acctcggtca atgcatcacg
     6661 caatacgagg taacccgcat cctgctcgga agcgatctcc ggattgccag cgctgccagt
     6721 cgccatcagg atcagcgaat cgttggtcga ggtgtcgccg tcgaccgtga tgcaattgaa
     6781 ggagcggttc accgcaaact ggatgatctg ttccagtgcc gcctggctga cggcggcatc
     6841 ggtcgcgata tatcccagca tggtcgccat gttgcggtgg atcatgccgg aacccttgga
     6901 gatcccggtg atggtgatgg tcttgccacc gaccgtgacc tgacgcgaaa cccctttggc
     6961 gacgatatcg gtggtcatga tcgcatccgc tgcgctacgc cagttgtctt ccttgaggtc
     7021 agcgatgcag ccgggcaagc cggcaagcaa gcggcccatg ggcaggggtt cgaggatgac
     7081 gccggtggag aacggcagga cttgctcggg tcggcatgcc agtagcgccg ccagctctgc
     7141 acaagtcgcg cgtgcgtcct gcaggcctga ttccccggtg ccggcgttgg cgttgccggt
     7201 attgaccact aaggcgcgga tgccggtatc ttgcgccaaa tgtgacttgc atagcgtgac
     7261 cggggccgcg cagaaacggt tcttggtgaa tacgcctgcc acccgcgtac cttccgccag
     7321 tttcatcacc agcatgtcct tgcggtctgg cttgcggata ccggccttgg cgataccgag
     7381 ctggacaccc ttgacgggca gaaggtcttg cgcctgtggc gcggatagat tcacgggcat
     7441 ggtgactttc gatgaggatg gcaaagcacc gattttaccg cattcgatgg cggaattagg
     7501 gcggccgcag aattgacatt gaggcaagct gtgatagtgt tggcatatcc aacgtctcag
     7561 ctgctcaggg gtcaacctgc atgaacaatc ccgctgtcgt acaggaaaaa gaagcggcca
     7621 gcctcacgga gatgggcaac aagctcgcct tcaccaaaaa gctgcaggcc gtcaccaaca
     7681 agatccacgc cacccgcaac atcgacgaga tcatcctcga ggtcagccag gacatccgcg
     7741 cgctattcga agccgaacga ctcacactgt acgtgacgaa cgatgacgag tccgccatcg
     7801 tctccaaggt caagaccggg ctcaacgaat tcaaggaact gaagctcgcc atcaacccgc
     7861 agagcatcgc cggttatgtc gccttcaaca agagcatcct taacatcgcc gatgtctacg
     7921 acgatgccga gctggaaagg atcagcccgg agatccattt cctcaaggaa gtcgacaaga
     7981 agaccggctt ccgcaccaag cagatgctga tcttccctat cctgagccag gaagacagtg
     8041 cgctcatggg ggtggtgcag gtgatcaaca ccatgtctgg cctgccgttc ccgcagatgg
     8101 cggaagaagg cgcgccggag ctggcaaaga cactggcgat cgcgctcagc cagcgacagg
     8161 cgccgatcgg cgtggtcaag accaagttcg ataccctggt cagcgaaggc atcatttcca
     8221 gcaccgaact cgaactggcg caacgctcgg cccgcaagaa agggaaggat ctcgaagagg
     8281 tcctgatcga ggagttccag gtcaaaccca cggatatcgg caaatcactc gccacgttct
     8341 tcggtgtcgc gttggagccg ttcaaggtcg accgcgtcaa gccgatggat ctgttgaaga
     8401 atctcaagcc ggattacgtc atgagcaacg gctggttgcc gatcgacgag accaaggaag
     8461 aggggctggt catcctcacc accgatccgg agaaagtgcg cgcctcgcgc atcgtcagca
     8521 acatcttccc gcgtcatgcc aagatcacct accgggtttg cctgcatcgt gacttcagca
     8581 acacggtcaa ccagttctat ggcgtcagcg gtctatctgg cgacatcgac gacatcctct
     8641 cgaccatggg cgaggacgag gaggacagcg gcggcatcgg cgccgaggat atctcggccg
     8701 ctgccgacaa cgagctggtc aagctcgtca acaagatcat catcgacgcc tataaccaag
     8761 gcgcgtccga catccatatc gagccttatc cggacaaggg taagaccgaa gtgcgcttcc
     8821 gtaaggacgg cacactggtc aattacatcg agatcccacc cagctaccgc aacgccatcg
     8881 tcgcccgcat caagatcatg tgcgaccttg atatcgccga gcggcgcaaa ccacaggacg
     8941 gcaagatcaa gttcaagaag ttcggcccgc tcgatatcga actgcgcgtc gccaccatcc
     9001 ccaccgcggg gggcatcgaa gatatcgtca tgcgcatcct ggcggcaggc aagccgatcc
     9061 cgctcgacaa gctcgggctc tcagagcaca acaacaagaa gctgaaggag ctggtcgcca
     9121 agccctacgg cctgttcttc gtctgcggcc cgaccggttc gggcaagacc accacgctgc
     9181 attctgcgct gggcttcatc aacaccccgg aaaccaagat atggaccgcg gaggacccgg
     9241 tcgagatcac gcagaagggc ctgcgccagg tgcaggtcaa caagaaggcc ggcctggatt
     9301 tcgccatggt gatgaaggcg ttcctgcgtg ccgacccgga cgtcatcatg gtcggcgaga
     9361 tgcgcgacaa ggaaacggtc tccaccggca tcgaggcctc gctcaccggc cacctggtat
     9421 tcgccacgct gcataccaat agcgcgcctg aatccatcgt gcgcctgctc gacatgggga
     9481 tggacccatt caatttcgcc gatgccatgc tcggcatcct ggcccagcgc ctgggcaagc
     9541 gtctgtgcga atgcaagaag gcctatacgc ccgatgccaa cgagatcaag ggcatgctca
     9601 acgaatattg cgaggaactg aagaacacct ccagctggaa gaaggatccg gaagccgcct
     9661 acaaagcggt ctaccagaat tgggtcaaga gttttggcaa cgacaagggt cagctcacgc
     9721 tatacaaggc gatcggctgt gacaaatgca acagcagcgg ctacaaggga cgcgtcggcc
     9781 tgcacgagct gctggaaggc accgacgcca tcaagaagaa catccaggaa catgctcggg
     9841 tggccgacat
//
LOCUS       DATMTW010000144         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_247425, whole genome shotgun sequence.
ACCESSION   DATMTW010000144 DATMTW010000000
VERSION     DATMTW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247425"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VN084_02690"
     CDS             <1..349
                     /locus_tag="VN084_02690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWT28393.1"
                     /translation="DHHGHLAGDMVIRRLAQIIRGCLRESDFLCRWGGEEYLLLMKGC
                     DLNRAQIIAETLRTTVKEAPIDYGDISIRITISLGVAEYHDPESNTAMFGRADEALYR
                     AKAAGRDCVRTAG"
     gene            complement(370..1584)
                     /locus_tag="VN084_02695"
     CDS             complement(370..1584)
                     /locus_tag="VN084_02695"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HWT28394.1"
                     /translation="MKVYQVGGAVRDALLGLPVKDTDHVVVGATPEQMARLGYRPVGK
                     DFPVFLHPESHEEYALARTERKTAKGYKGFQVFASPEVTLEEDLARRDLTINAIARDE
                     AGQLIDPFNGQADISSRTLRHVSEAFAEDPVRILRAARFAARFTDFSVAPETMRLMKG
                     MVDAGEVDALVAERVWQELSKGLMEAKPSRMFDVLRECGALQRIFPELDRLWGVPQPP
                     QHHPEIDTGVHVMMVIDYAASQGYSLPVRFAALTHDLGKGTTPAEILPRHIGHEERSV
                     SLLKDVCKRLRVPNDCKELAHIVARFHGKVHAVSEMRPGTLVQFLQDTDAIRQPQRFR
                     EFLLACESDSRGRTGFEQKPFPQAERLLQLLAAVTAVDAGAIAQQFDAPEKIKAAVFE
                     ARVEAVKALSQA"
     gene            complement(1715..2662)
                     /locus_tag="VN084_02700"
     CDS             complement(1715..2662)
                     /locus_tag="VN084_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HWT28395.1"
                     /translation="MAIRQVCVLGGSGFVGSAIVHRLSAAGYDVKVLTRRRERAKHLI
                     LLPYVQVIECDIMDDAALSQQISGTDAVINLVGILHESSKSSFNGIHHELAERVAKLC
                     RRHKVARLLHMSALQAAADAPSAYLRSKAAGESAVIAEAGETKVTIFRPSVVFGRGDG
                     FLNLFANLVKVMPVVVLAKPEARFQPVWVEDVAQAFVASLDNTTTYGKVYELAGPRVY
                     TLRELIEFVVFVLGKKRKVVGLNDKLSYYQAYLLEKMPIKLMTRDNLASMEVDSVASG
                     PFPAVFGFKPTALEAVVPEYLANDTPRGAYDRFRGLAGR"
     gene            complement(2688..>4201)
                     /locus_tag="VN084_02705"
     CDS             complement(2688..>4201)
                     /locus_tag="VN084_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWT28396.1"
                     /translation="GEPPASCDSLFDRMQKAGVLTEEDIWQRFRLALVEGKLPLAKAI
                     LQRMPNATKANLKLIDSVYANPQRALDKKSISLKSRLGRELNLYALERVSRSQMAVAL
                     KHWGHMQASYDAGDRSYLWSRFALYAARQHDPAALEYYTRGSDATLDKEQIAWKVRAA
                     LRIKDWPGVQAAIGEMPVSQQQESAWRYWKARALKEQQQIPQANAIFVPLSHERSYYG
                     LLAEEELGPVMSEPPSFYKASEADIKEVAALPGIQRALELQRQELRWESRSEWAMATK
                     DFDDRLMIAAAEIAARQEWYDLAIITADKTRFTHDYTLRYPTPYRDLVRSFSKDQGLD
                     EAWVYGLARQESRFIIQAKSGVGAAGLMQVMPATARWIAKRMGLDSYHNGMIHQLETN
                     IQMGTYYMRHVLDQMGGQALMATAAYNAGPGRAKRWADPQPMEGAIYAETIPFSETRN
                     YVQKVMANAYFYASRLGTKVQSVKQRLGVIAGTASDNVNPDDETVVAEQNKPE"
BASE COUNT          751 a         1322 c         1274 g          854 t
ORIGIN      
        1 cgaccatcat ggtcacttgg caggcgacat ggtcatccgg cgcctggccc agatcatccg
       61 tggctgcctg cgcgaatccg atttcctctg ccgctggggc ggggaagaat acctgctgct
      121 gatgaaaggc tgcgacctca accgtgcaca gatcatcgct gagacgctgc gcaccaccgt
      181 gaaggaggcg ccgatcgatt acggcgacat cagcatcagg atcaccatca gcctcggcgt
      241 cgcggaatac catgacccgg aatcgaacac ggcgatgttc ggccgagctg acgaagcgct
      301 ctatcgtgcc aaggccgctg gccgtgactg cgtccgtact gccggatgag tgtgcggaat
      361 acctgggact taggcctgcg acaatgcctt gaccgcttcg acgcgggctt cgaacacggc
      421 agccttgatc ttctccggcg cgtcgaactg ttgggcgatt gcgccagcat cgaccgctgt
      481 caccgctgcc agcagctgca ataagcgttc ggcctgcggg aatggtttct gctcgaagcc
      541 agtgcgcccg cgcgaatcgc tctcacaggc cagcagaaat tccctgaacc gctgtggctg
      601 ccggatcgcg tccgtatcct gcaggaactg cacaagtgtc cccggccgca tctccgagac
      661 cgcatggacc ttgccatgga acctggcgac gatatgggcc agctccttgc aatcgttggg
      721 tacgcgtaag cgcttgcaca cgtccttcag caggctcacg ctgcgctcct cgtgaccgat
      781 atggcgcggc aggatctccg ctggcgtcgt gcccttgccg aggtcgtgcg tcagcgcggc
      841 gaatcgcacc ggcagggaat acccttgtga tgccgcgtaa tcgatgacca tcatcacatg
      901 cacgccggta tcgatctcgg gatgatgttg cggcggttgc ggcacgcccc agagccgatc
      961 gagttcgggg aatatccgct gcagggcgcc gcactcccgc agcacgtcga acatgcgcga
     1021 gggcttcgcc tccatcaggc ccttggacaa ttcctgccag accctttcgg ccacaagcgc
     1081 atcaacttcc cccgcatcga ccatgccttt catcagtcgc atggtctcgg gcgcgacact
     1141 gaagtcggtg aagcgtgccg cgaaacgtgc cgcacgcaga atgcgcaccg ggtcctcggc
     1201 gaaggcttcg ctcacgtgac gcaaggtcct gctggagata tcggcctggc cgttgaatgg
     1261 gtcgatgagc tgacccgctt cgtcgcgggc gatggcgttg atggtgagat cgcgacgtgc
     1321 gaggtcttct tccaaggtga cttctggcga agcgaacacc tggaaaccct tgtaaccttt
     1381 tgccgtcttg cgctcagtgc gtgccagcgc gtattcctcg tgcgattccg gatgcaggaa
     1441 cactgggaaa tccttgccta ccggtcgata accgagtcgc gccatctgct caggcgtggc
     1501 gcccacgacg acatggtcgg tatccttgac cggcaacccc agcaaggcat cacgcacagc
     1561 accgccgacc tggtagacct tcatctgcat gctcctgcgt gaatcgcatt gcatggcggg
     1621 atgccctgcc acacggaacg cacagacgcg tattcaccca tgtgaaggtg aggagtcttc
     1681 gccccgcgcg atgccgtcac cagcatctat ggcctcaacg tccggcgagg ccgcggaatc
     1741 ggtcataggc gcccctgggg gtatcattgg ccagatactc cggcaccacg gcttcgagcg
     1801 ccgtcggctt gaaaccgaac accgccggga acggaccgct ggcgacacta tcgacttcca
     1861 tcgaagccag gttgtcgcgc gtcatgagct tgatcggcat cttttccagc agataggcct
     1921 gatagtagga gagcttgtca ttgaggccga cgaccttgcg cttcttgcca aggacgaaga
     1981 ccacgaactc gatcagttcg cgcaaggtgt agacgcgcgg gccggccagt tcatagacct
     2041 tgccgtaggt ggtcgtgttg tcgaggctag cgacgaacgc ctgggcgaca tcttccaccc
     2101 acactggctg gaagcgggct tcaggcttcg ccagcacgac gaccggcatg accttgacca
     2161 gattggcgaa caggttgaga aaaccatcgc cgcgaccgaa caccaccgat ggccgaaaga
     2221 tggtgacctt ggtctcgcca gcttcggcga tgacggcgga ttcgccggcc gctttcgacc
     2281 gcaggtaggc actgggcgcg tcagcggcgg cttgcagggc actcatgtgc agcaggcgcg
     2341 ccaccttatg acggcgacag agctttgcga cgcgttccgc cagttcgtga tggatgccat
     2401 tgaaactgga tttgctgctc tcgtgcagga tgccgaccag gttgatgacg gcatcggtcc
     2461 ctgagatctg ttgcgacagc gccgcgtcgt ccatgatgtc gcattcgatg acttgcacat
     2521 agggcagcag gatcaggtgt ttggcgcgct cacgcctgcg ggtcagcacc ttgacgtcgt
     2581 aacctgccgc actgagccga tggacgatcg cactgccgac gaaacctgaa ccacccaata
     2641 cacatacctg cctgattgcc attgctatcc tctgttgtcc tgattgttca ttccggctta
     2701 ttctgttcag cgactacagt ttcatcgtcc gggttcacgt tatcactcgc cgtgcctgcg
     2761 atgacaccca gccgctgctt cacgctctgc accttggtac cgagccggct ggcgtagaaa
     2821 taggcattgg ccatgacttt ctgcacgtag ttgcgcgtct cgctgaacgg gatggtctcg
     2881 gcatagatcg cgccctccat cggttgcggg tccgcccagc gcttggcacg gccagggccg
     2941 gcgttgtagg ctgcggtcgc catcagcgcc tggccaccca tctggtccag cacatgccgc
     3001 atgtagtagg tacccatctg gatattggtc tcgagctgat ggatcatgcc attgtggtaa
     3061 ctatcgagcc ccatgcgctt ggcgatccac ctcgccgtgg ctggcatcac ctgcatgagg
     3121 ccggcggcgc cgacgccgga tttggcctgg atgatgaagc ggctctcctg cctggccagt
     3181 ccgtagaccc aggcctcgtc cagcccctgg tccttggaaa agcttcgcac caggtcacga
     3241 tagggcgttg ggtagcgcaa ggtgtagtcg tgggtgaatc tggtcttgtc ggcagtgatg
     3301 atggcgaggt cataccactc ctgccgtgcc gcgatctccg cggcggcgat catcaggcga
     3361 tcgtcgaaat ccttggtcgc catggcccat tccgaccggg actcccagcg cagctcctgg
     3421 cgctgcaact ccagcgcacg ctggataccg ggcagcgctg cgacttcctt gatatccgcc
     3481 tccgaggcct tgtagaagct cggtggctca ctcatcaccg gccctagctc ttcttctgcc
     3541 agcaggccgt aatagctgcg ctcatgcgac agcgggacga agatggcatt ggcttgcggg
     3601 atctgctgtt gttctttgag cgcccttgcc ttccagtaac gccaggcgga ttcctgctgc
     3661 tggctgaccg gcatctcgcc gatggctgcc tgcacgcccg gccaatcctt gatgcgcagc
     3721 gcggcacgca ctttccaggc gatctgttcc ttgtccagcg tggcatcgct accgcgtgtg
     3781 taatattcga gcgcggccgg atcatgctgg cgcgcggcat agagcgcgaa gcggctccac
     3841 agataactgc gatcaccggc gtcgtaggac gcttgcatat ggccccagtg tttgagggcg
     3901 accgccatct ggctacgcga gacacgctcc agcgcataga ggttgagctc acgtcccagg
     3961 cggctcttga gggagatgct cttcttgtcg agcgcgcgct gcgggttggc gtagacgctg
     4021 tcgatcagct tgagattggc cttggtggcg ttcggcatgc gctgcaggat cgctttggcc
     4081 aaggggagtt tgccttcgac cagggccagc cggaaccgct gccagatatc ctcttccgtg
     4141 aggacaccgg ctttctgcat gcgatcgaac aggctatcgc agctggctgg cggttcgccc
     4201 g
//
LOCUS       DATMTW010000145         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_549692, whole genome shotgun sequence.
ACCESSION   DATMTW010000145 DATMTW010000000
VERSION     DATMTW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549692"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VN084_02710"
     CDS             <1..1246
                     /locus_tag="VN084_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWT28397.1"
                     /translation="ALGGGLEIYDFIIFVFFALTLSQLFFPPEMPEWLRLLQSFGIFV
                     TGYLARPLGGILMAHFADRLGRKKVFSLSILMMALPCLLIGIMPTYAQIGYFAPLLLL
                     ALRILQGAAVGGEVPSAWVFVAEHAPIAHRGYALGFLQAGLTFGYLIGALTATFLAQA
                     FSPAEILDYAWRYPFLLGGVFGVIGVWLRRWLSETPVFMAMQAQREAAGELPLRTVLR
                     EHRLAILPAMILTCVLTSAVVVFVVITPTMMQKTFGMTASHTFALSALGIVFLNIGCV
                     LAGLLVDRIGAWRTVMLYSLLLPLGIGVLYACLIMGGDWIGLAYAVAGLACGVVGAVP
                     SVMVSLFPARIRVSGISFTYNIAYAAWASITPLLLIGLMPWSPWICVMFSAVMGAVGV
                     GSAAYFGARMPRIAGCSVAGAH"
     gene            1364..>1656
                     /locus_tag="VN084_02715"
     CDS             1364..>1656
                     /locus_tag="VN084_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWT28398.1"
                     /translation="MFNKRLKQELSALREELSSLQQVKESLESEMLVLTLDSDGRVQS
                     VNQNFLGEMHYKSADLIGRHIDDLVPDHVKSDEFQLRFKNALTRGEHFAGAVRL"
BASE COUNT          261 a          487 c          523 g          385 t
ORIGIN      
        1 cgccctcggc gggggactgg aaatctacga tttcatcatc ttcgtgtttt tcgccctgac
       61 cctcagtcag ttgttcttcc cgccggaaat gcccgagtgg ctgcgcctgc tgcaaagctt
      121 cgggatcttc gtcaccggtt acctggcgcg gccgttgggc ggcatcctga tggcgcattt
      181 cgccgaccgt ctggggcgca agaaagtctt cagcctgagc atcctgatga tggcgttgcc
      241 ctgcctgctg atcgggatca tgccgaccta cgcgcaaatc ggctatttcg caccgttgtt
      301 gctgttggcc ctgcgcatcc tccagggcgc ggcggtgggc ggcgaggtgc ccagtgcctg
      361 ggtattcgtc gccgaacacg cgcccatagc ccatcgcggc tatgccttgg gcttcctgca
      421 agcggggctg acctttggtt acctgatcgg tgcattgacc gcgactttcc tggcgcaggc
      481 attcagcccg gcggaaatcc tcgattacgc ctggcgctac ccattcctgc tcggcggcgt
      541 gtttggggtg attggcgtgt ggctgcgtcg ctggctcagc gaaaccccgg tgttcatggc
      601 catgcaggcg caacgcgagg cggccggcga attgccgctg cgcacggttt tgcgcgagca
      661 tcgcctggcc attttgccgg cgatgattct cacctgtgtg ctgacctcgg ccgtggtggt
      721 gttcgtggtc atcaccccga ccatgatgca gaaaaccttt ggcatgaccg ccagccatac
      781 cttcgccttg agcgcgctgg gcatcgtgtt cctgaacatc ggctgcgtgc tcgccgggct
      841 gctggtcgat cgcatcggcg cctggcgcac agtgatgctc tatagcctgc tgctgccgct
      901 gggcatcggc gtgctgtatg cctgcctgat catgggcggc gactggatcg gcctggccta
      961 tgcggtggca gggctggcct gcggggtggt cggcgcggtg ccatcggtga tggtcagcct
     1021 gttcccggcg cgaattcgtg tctcgggcat ctcgttcacc tacaacattg cctacgccgc
     1081 gtgggcaagc atcacaccgc tgttgttgat cggtctgatg ccgtggagtc catggatctg
     1141 cgtgatgttc agtgcggtga tgggcgcggt gggcgtgggc agtgcggcgt acttcggtgc
     1201 gcggatgccg agaatcgcag gctgttctgt ggctggcgcg cattgatcgg gcgtgtcgta
     1261 actatttttg taggacaaac ctgaagcacg cttaagaaaa tgtttccaag gccgatggtt
     1321 aggctgcata tcgctttcta tccctgtcca tcggtgcttt cccatgttca ataaacgctt
     1381 gaagcaggag ctgtcggctc ttcgtgaaga actctccagc ctccagcaag tgaaggaaag
     1441 tctggaaagc gaaatgctgg tgctgaccct cgacagcgac gggcgggttc agtcggtcaa
     1501 ccagaacttc cttggcgaaa tgcactacaa gagtgctgac ctgatcggcc gacacatcga
     1561 cgacctcgtc ccggatcacg tcaaatccga tgaatttcaa ttgcgtttca agaacgcgct
     1621 gacccgtggc gagcacttcg ctggcgcggt gcgcct
//
LOCUS       DATMTW010000146         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_448900, whole genome shotgun sequence.
ACCESSION   DATMTW010000146 DATMTW010000000
VERSION     DATMTW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448900"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1529)
                     /locus_tag="VN084_02720"
     CDS             complement(18..1529)
                     /locus_tag="VN084_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWT28399.1"
                     /translation="MNKRPSQEFNLSSETNTPSLSFNELGLAQPILRAIADAGYTTPT
                     PIQAKAIPQVLSGGDLLAGAQTGTGKTAGFTLPLLHLLQAKPAQMQAGQPRCLILTPT
                     RELAAQVEESVKTYGKYLPLKSMVMFGGVNINPQIARLKKPLDILVATPGRLLDHVGQ
                     KTIDLSRIEILVLDEADRMLDMGFIRDIKRVLALLPKQRQNLLFSATFSDEIKALADG
                     LLHNPGFVEVARRNTASELVEQTVHLVPQAHKRDLMSHLIRQHDWQQVLVFTRTKHGA
                     NRLAEKLVKDGIEAAAIHGNKSQGARTKALADFKSGSVKVLVATDIAARGLDIDQLPQ
                     VVNFELPNVPEDYVHRIGRTGRAGSTGAAISLVDEEEVKLLKAIERLIKREIPRVTVE
                     GFVPSEKTVEAPRPPKPARGQGQQPRGRDGRNQARSSSGQNGQGQAGQRQGKPGHGTN
                     KGSGNARPASQPATRSDNQPKNAQPMSEAARHQAMPQSPLFAPKPVHRGHRGR"
     gene            1684..1854
                     /locus_tag="VN084_02725"
     CDS             1684..1854
                     /locus_tag="VN084_02725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT28400.1"
                     /translation="MECIAREEGTPINFTISIGVSSLKDKGTTLDVLLSQVDAAMYEA
                     KQSGRNKVTIAT"
BASE COUNT          348 a          565 c          625 g          441 t
ORIGIN      
        1 agctcctgcg agggttttca gcggccgcga tgaccgcggt ggaccggttt gggggcgaac
       61 agcggcgact gtggcatggc ctgatggcgg gctgcttcag acatcggctg tgcgttcttg
      121 ggctggttat ccgatcgcgt cgctggctgg ctggcgggac gtgcgttgcc gctgcccttg
      181 ttcgtgccgt gccctggctt tccctgtcgc tgcccggctt gtccctggcc attctggccg
      241 gacgacgaac gcgcctgatt gcggccgtca cggccacgtg gctgctgtcc ctggcctctt
      301 gccggtttgg gaggacgcgg tgcctcgaca gtcttttcgc taggtacgaa gccttctacc
      361 gtgacgcgtg gaatctcgcg tttgatcagg cgttcgatcg ctttcagcaa cttcacttct
      421 tcttcatcga cgagcgagat ggcggcgccg gtgctgcctg cgcggcctgt tcgtccgatc
      481 cggtggacgt agtcctccgg tacgttgggt aattcgaaat tcaccacttg cggcaattgg
      541 tcgatatcga gtccgcgtgc ggcgatatcc gtcgccacca ggaccttgac actaccgctc
      601 ttgaaatcgg ccagcgcctt ggtacgcgcg ccctgactct tgttgccgtg gatcgctgcg
      661 gcctcgatgc cgtccttgac cagcttttcg gcgaggcgat tggccccgtg cttggtgcgc
      721 gtgaacacca gtacctgctg ccagtcatgc tggcggatca gatggctcat caggtcgcgc
      781 ttgtgcgctt gcggcaccag gtgcacggtc tgctcgacca gctccgaagc cgtgttgcga
      841 cgcgccactt cgacaaagcc cggattatgc agcaagccat cggccaacgc cttgatctca
      901 tcggagaatg tagccgagaa caacaggttc tggcgctgtt tcggcagcaa ggcgaggaca
      961 cgtttgatat cacggatgaa gcccatgtcc agcatgcgat cggcttcgtc cagcacgagg
     1021 atctcgatgc gggagaggtc gatggtcttc tggccgacat ggtcgagcag ccggccgggc
     1081 gtcgccacca ggatatccag cggtttcttc aggcgagcga tctgcggatt gatattgacg
     1141 ccgccgaaca tgaccatgga tttcagcggc aggtatttgc cgtaagtttt caccgattcc
     1201 tcgacctgag ccgcgagctc gcgcgtcggc gtcagaatca ggcagcgtgg ctggccggct
     1261 tgcatctgcg cgggctttgc ctgcaatagg tgcaacagcg gtagcgtgaa accggcggtc
     1321 ttgccggtac cggtctgcgc gccggcaagc aagtcgccgc cgctcaatac ctgcggaatg
     1381 gccttggcct ggatcggtgt cggcgtggtg tagccggcat cggcgatcgc acgcaagatg
     1441 ggttgagcca gcccgagttc gttgaaggac aggctggggg tgtttgtttc agatgacaag
     1501 ttaaactcct gcgacggcct tttgttcatg gaacaaaacc aatcgaggca gacgaataag
     1561 ggatcgagta gatcggagta gggaatagac cgctatgctg attggtgtta catagggaag
     1621 ctgcattata cgcagtttca cccgcaaggt aagtcgagag ttggttttat tgtatgtaaa
     1681 tcagtggaat gcatcgcacg ggaagaaggc acgccgatca atttcactat ctcgatcggg
     1741 gtatctagcc tgaaggataa aggcaccacc cttgatgtct tgctgagcca ggtagatgcc
     1801 gcgatgtatg aggccaagca gtcagggcgc aacaaggtca ccatcgcgac ctgagcgtct
     1861 tgtcaggctg gcagcttctc gcccctgagg ttttccatgt ccgtttcatc gagccagcgc
     1921 cattcgcccg gcgccaggtc cgccggcaac tccagcgcgc cgatccggat acgcttgag
//
LOCUS       DATMTW010000147         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_381748, whole genome shotgun sequence.
ACCESSION   DATMTW010000147 DATMTW010000000
VERSION     DATMTW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381748"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VN084_02730"
     CDS             <1..637
                     /locus_tag="VN084_02730"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWT28401.1"
                     /translation="QGCGSCPPADAAFRKIVGQGDVIALAYHVDYWNYLGWTDTLSSK
                     QNTDRQYGYARMLGRSNVYTPQVVVNGRDHMAGSDISAINGKINGFSTEGKGLSVPVD
                     AQMRGDELEIKIGAGQGKASVVMVYFDKEKTIDVEKGENSGQKISYINSVSDIETVGM
                     WDGKATSLTLPATVLQKADLEGCAILLQAATADGDPAAILGATVIMAGKNI"
     gene            916..1317
                     /locus_tag="VN084_02735"
     CDS             916..1317
                     /locus_tag="VN084_02735"
                     /inference="COORDINATES: protein motif:HMM:NF022437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HWT28402.1"
                     /translation="MTDQPDLRHGDHRSADNSSSVVVDLKEYKQSKDPLPVTFHRREL
                     DAILWIYGRMVGEGEWKDYAIDHMKDKAVFSVFKRSGEMPLFTIEKDPKLANKQGAYS
                     VVNTNGMILKRGHELQQVLKVFDKVLKLVDK"
     gene            complement(1322..>1694)
                     /locus_tag="VN084_02740"
     CDS             complement(1322..>1694)
                     /locus_tag="VN084_02740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWT28403.1"
                     /translation="GELLGYAYASAFRTRPAYRWMVEDSIYLAPAARGKGVGKALLAE
                     LISRCTALGFRQMVAVIGGAHPASIALHRSSGFEETGLLKGTGYKHGRWLDTMLMQRV
                     LGEGKDTDPDLSAYPGSLYKG"
BASE COUNT          366 a          483 c          529 g          316 t
ORIGIN      
        1 acagggctgc ggctcctgtc cgccggctga tgcggcgttt cgcaagatcg tcggccaggg
       61 cgatgtcatc gcgctcgcct atcatgtcga ttactggaac tatctcggct ggaccgacac
      121 gctgagctcc aagcagaata ccgaccggca gtatggctat gcccggatgc tcggccgcag
      181 caacgtctat acgccgcagg tggtggtcaa tggccgcgac catatggcag gatccgacat
      241 ctcggcgatt aacggcaaga tcaatggctt ctccacggag ggcaagggcc tctccgtgcc
      301 cgtcgatgcg cagatgcgcg gcgacgagct ggagatcaag atcggtgccg ggcagggtaa
      361 ggccagcgtc gtcatggtct atttcgacaa ggaaaagacc atcgacgtcg aaaagggcga
      421 gaatagcggc cagaagattt cctacatcaa cagcgtcagc gacatcgaaa ccgtgggcat
      481 gtgggacgga aaggcaacaa gcctgacctt acctgcaacg gtgctccaga aggccgacct
      541 cgagggctgt gcaattctgc tgcaggctgc tacggctgat ggtgacccgg ccgcgatcct
      601 gggagcaacc gtcatcatgg cgggcaagaa tatctgaggg tttcatctgc tccggcagat
      661 ggaaaacggg gcggcccggc tgatcgtatt cggggccggg gttaaggtcg ataggctcag
      721 ccgggccctt tggcgcgctg gcgccaaatc ggtgagcgga acttcccctg caaatgaggc
      781 gctgttttga ctgaattgcg gcgctcagga gattgcatag catatgatta atgtgatgac
      841 tggccgcact tgcaatttgg cgcggctcgg gcaaactgtc actctcctgt cacaagcgga
      901 ggcacaggga gactgatgac agatcagccg gatttgcggc atggcgatca ccgttccgcc
      961 gacaatagtt cctcagtcgt cgtcgatctt aaagaataca agcagagcaa ggatccgttg
     1021 ccggtgacgt ttcaccggcg agagctggat gcaatcctgt ggatttacgg ccgcatggtc
     1081 ggcgagggcg agtggaaaga ctacgcaatc gaccacatga aggacaaggc ggtgttctcc
     1141 gtcttcaagc gctccggcga aatgcccctc ttcaccatcg agaaagatcc caagcttgcc
     1201 aacaagcagg gcgcctattc cgtggtgaat accaacggca tgatcctgaa gcgtggccac
     1261 gagctgcagc aggtcctgaa ggtcttcgac aaggttttga agctcgtcga caaataggcg
     1321 cttagccctt gtagaggctg cctggatagg cagagagatc cgggtctgta tccttgccct
     1381 cgccgagcac acgctgcatc agcatcgtgt ccagccagcg gccgtgcttg tagccggtgc
     1441 ccttcagcag gccggtttcc tcgaaacccg atgaacgatg cagcgcgatc gaggctggat
     1501 gggcgccgcc gatgacggcc accatttgcc ggaagccgag cgcggtgcag cgtgatatca
     1561 gttccgccag cagcgccttg ccgacgccct ttccacgggc ggcgggggca agatagatcg
     1621 aatcctcgac catccatcta taggccggcc gcgtgcggaa cgccgacgca taggcatagc
     1681 ccagaagctc gccg
//
LOCUS       DATMTW010000148         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_348816, whole genome shotgun sequence.
ACCESSION   DATMTW010000148 DATMTW010000000
VERSION     DATMTW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348816"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VN084_02745"
     CDS             complement(<1..729)
                     /locus_tag="VN084_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWT28404.1"
                     /translation="MIPSALVFVLGVWVLQQQSALPSPFWLLSAIPLIGLAVVSRRRY
                     PAVMHLSLAGLCFLAGFFWAAMLAQMRLHDALPQAWERKDIALVGVIASLPQSHERGQ
                     RFEFDVERVLTPGAIVPTHISLTDYGTGYANPEAKPGHDARMTESPLSRFQAGERWQL
                     TVRLKRPHGTSNPHNVDFEAWAFERDIRAAGYLRAAGDSRKLDAFVYRPGYVVDRLRG
                     HIRNHLDAVLAGAPYAGILRALAIG"
     gene            complement(736..1407)
                     /gene="lolD"
                     /locus_tag="VN084_02750"
     CDS             complement(736..1407)
                     /gene="lolD"
                     /locus_tag="VN084_02750"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HWT28405.1"
                     /translation="MTAIISCTHLQKTYTGLDVAVLNGIDLTIGAGEQVAIVGASGSG
                     KSTLLHLLGGLDDVTAGHVELLGHAMDSLSETRRGQLRNEALGFVYQFHHLLPEFTAL
                     ENVAMPLLIRRLPRDAALASAAAVLRKVGLGHRLEHMPGELSGGERQRAAVARALVTQ
                     PRCVLADEPTGNLDRHTADGVFDLLLQLNAEQGVSLVVVTHDLQLAARMQRQYKLEDG
                     KLHAI"
     gene            complement(1524..2792)
                     /locus_tag="VN084_02755"
     CDS             complement(1524..2792)
                     /locus_tag="VN084_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWT28406.1"
                     /translation="MSRSRNLPFELFVGLRYTRAKRRNHFISFISMTSMIGIGLGVAA
                     LIVVLSVMNGFQEELRERILGVASHLQITGDNNVLKDWQTVAAKVQHAPHVEGTAPYI
                     MAQGMLSYGQAVQGALVRGILPDAEDRVADIGQHMKAGRLADLRPGEFGIVLGNELAQ
                     SLGVLLGDKVVLMAPQGQFTPTGVVPRIKQFKVVGIFEIGMYEYDAGLALIQMDDAAK
                     LYRMGDNVSGLRLKLDDLFHAPAISQALGDLIGKSSETYYLTDWTQQHANFFRAVQME
                     KRVMFLILALIVAVAAFNIVSTLVMAVTDKRADIAIMRTFGASPRSIMQIFIVQGALI
                     GVIGTVFGAVAGVILALNIETVVPFIEHTFGVQFLAKDVYYINDLPSRLDWMDVTVIT
                     TMSFVLSLLATLYPSWKAAKTNPAEALRYE"
     gene            2842..3450
                     /locus_tag="VN084_02760"
     CDS             2842..3450
                     /locus_tag="VN084_02760"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWT28407.1"
                     /translation="MFTGIVQAVGQIKDIAPAGADVKLTIATAGLDLEDVAVGDSIAV
                     NGVCLTATQLGVGEFHAHVSHETLSCTVGLDSPRPVNLEKALRFSDRLGGHLVSGHVD
                     GVGQIVRFEPVDDCMLLAVRAPHALSRYIAVKGSIAIDGVSLTVNHVEKDTFSVNLIP
                     HTLEMTSLRTLGIGSRVNLEIDLIARYVERMAEWQRDGGDSA"
     gene            3447..>3937
                     /gene="ribB"
                     /locus_tag="VN084_02765"
     CDS             3447..>3937
                     /gene="ribB"
                     /locus_tag="VN084_02765"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWT28408.1"
                     /translation="MSPTSSLVSPINEIIEEIRAGRMVILVDEEDRENEGDLVLGAQF
                     TTPAHINFMAKHGRGLICLTLTEDRCKQLRLPAMVQKNGARMGTNFTISIEAAEGVTT
                     GISAADRARTIQAAVARQASPKDIVSPGHVFPLVAENGGVLVRAGHTEAGCDLASLAG
                     LEPA"
BASE COUNT          811 a         1227 c         1194 g          705 t
ORIGIN      
        1 accgatcgcc agcgcccgca agatacccgc atagggcgcg ccggcgagca ccgcgtccag
       61 atgatttcgg atatgcccgc gtaaccggtc taccacatac cccggacgat agacgaaagc
      121 gtccagttta cggctgtcac cagcagcgcg cagatacccg gcggcccgga tgtcacgctc
      181 gaaagcccag gcttcaaaat cgacattgtg tggattggac gtgccatgcg gtcgcttgag
      241 ccggaccgtg agctgccagc gttcaccagc ctggaatcgg ctcagcggcg actccgtcat
      301 ccgggcatca tgccccggct tcgcttccgg attcgcatag cccgtaccat agtccgtgag
      361 tgagatatgt gtcggtacga tcgcaccggg ggtcaggaca cgctccacgt cgaattcgaa
      421 tcgctgtccg cgctcatgcg actgcggcaa gctcgcgatc acgccaacga gcgcgatgtc
      481 tttacgttcc catgcctgcg gcaaggcgtc atgcagtcgc atctgagcca gcatggcggc
      541 ccagaagaat ccggccagaa aacatagtcc ggccagcgag agatgcatga ccgcggggta
      601 gcgacgccgg gagactactg cgaggccgat caggggaatg gctgaaagta gccagaatgg
      661 cgacggtagc gccgattgtt gttgcaggac ccaaacaccc aggacgaaaa ccagtgccga
      721 tgggatcatc gccggctaga tggcgtgcag cttgccgtcc tcgagcttgt attgccgttg
      781 catgcgcgcc gccagttgca gatcatgggt gacgacgacg agactgacgc cctgctcggc
      841 gttcaactgc agcaagagat cgaaaactcc atcagcggta tggcggtcga gattgccagt
      901 cggttcatcg gcaagcacac atcgcggctg cgttaccagc gcccgcgcca cggctgcgcg
      961 ctggcgctcg cctccagaga gctctcccgg catatgctcc agccgatgac ccaacccgac
     1021 cttgcgcagc acggcggcgg cagacgccag cgccgcatca cgcggcaggc gacggatcag
     1081 gagtggcatc gccacattct cgagggccgt gaattccggc aacaggtgat ggaactgata
     1141 gacgaagccg agcgcctcgt tgcgcaactg gccgcgcctg gtctcgctca gggaatccat
     1201 cgcgtgtccc agcagctcga cgtggcctgc ggtcacgtca tcgagtccgc ccaaaagatg
     1261 tagcaacgtg ctcttgccag aaccggaagc gccgacgatg gcgacttgct caccggcgcc
     1321 gatcgtcagg tcgatgccat tgagcacggc gacatcgagt ccggtgtagg tcttttgtaa
     1381 atgggtacag gagatgatgg cggtcataag gctacattcc agcggcaagc gcggcgaaca
     1441 gcttgtaacc attgcgcgag cagcatgcct gtgcgcgcaa cgaagtcacc gacaacgccg
     1501 caggccggct acacgacagt ttgctattca taacgcaaag cctctgcagg attggtcttt
     1561 gccgctttcc agctcgggta tagcgtcgcc agcaggctca gtacgaacga catggtggtg
     1621 atcaccgtga cgtccatcca gtccagccgc gacggcaggt cgttgatgta atacacatcc
     1681 ttggccagga attgcacgcc gaaggtatgc tcgatgaaag gcacgacagt ctcgatattg
     1741 agcgcaagga tcacgccggc aacggcgcca aacacagtgc cgatgacacc gatcagcgcg
     1801 ccctgcacga tgaagatctg catgatgctg cgcggactgg cgccaaaggt acgcatgatg
     1861 gcgatgtcgg cgcgcttgtc ggtcactgcc atgacgagcg tggacacgat attgaacgcc
     1921 gccacggcga cgatgagcgc gaggatgagg aacatcacgc gcttctccat ctgcacggca
     1981 cggaagaaat tggcgtgttg ctgagtccag tcggtgaggt agtaagtctc gctcgacttc
     2041 ccgatcaggt cgcccaatgc ctggctgatg gctggcgcat ggaacaggtc atcgagcttg
     2101 aggcgcagac cggagacatt gtcgcccatg cggtaaagct tggcggcatc gtccatctgg
     2161 atcaaggcga ggcctgcgtc atattcatac atgccgatct cgaagatacc cactaccttg
     2221 aactgtttga tgcgcggcac cacgcccgtc ggcgtgaact ggccttgagg cgccatcagc
     2281 acgaccttgt cgccgagcag gacacccagg gattgcgcca gctcgttgcc gagtacgatg
     2341 ccgaattcgc ctggccgcaa gtctgcgaga cgcccggctt tcatgtgctg gccgatatcc
     2401 gccacgcgat cctcagcgtc cggcaagata ccgcgcacca atgcgccttg caccgcctgc
     2461 ccgtaggaaa gcatgccctg cgccatgatg tagggcgccg tgccctcgac gtgcggcgca
     2521 tgctggacct tggccgccac cgtctgccag tccttgagca cgttattgtc gccggtgatc
     2581 tgcagatgcg aggcgacgcc aaggatgcgt tcgcgcaact cctcctgaaa cccgttcatg
     2641 accgagagca cgacgatcag cgcggcaacg ccgagaccga tgccgatcat gctggtcatg
     2701 gaaatgaacg agatgaagtg attgcggcgc ttggcacggg tataacgcaa gccgacaaac
     2761 agttcgaatg gcaggtttct ggagcgtgac atagcgacga attctagcag ggttgcggcc
     2821 tcattctgtt aaaattgtgg catgttcacc ggaatcgtac aggccgtcgg ccagatcaag
     2881 gacatcgccc ctgcgggtgc cgatgtcaag ctcaccatcg ctaccgctgg cctcgacctc
     2941 gaggatgtcg cggtcggcga cagcatcgcc gtcaacggcg tctgtcttac cgccacacag
     3001 ctgggcgtgg gtgaattcca cgcccatgtc tcgcacgaga ccttgtcgtg cacggttggc
     3061 ctcgacagcc cgcgcccggt caatctggag aaagcgctgc gtttctccga tcgccttggc
     3121 ggccaccttg tcagcggcca cgtcgatggc gtcggtcaga tcgtacgttt cgagcctgtg
     3181 gacgactgca tgctgctcgc tgttcgggcg ccgcacgcgt tgtcccgtta catcgccgtc
     3241 aagggctcga tcgccatcga cggcgtcagc ctgaccgtca atcatgtcga aaaagacacc
     3301 ttcagcgtca atctcatccc gcacacgctg gagatgacat cgctcagaac cctggggatc
     3361 ggtagtcgcg tcaacctcga aatcgacctt atcgcccgct atgtcgagcg tatggccgaa
     3421 tggcaacgcg acggcggaga cagcgcatga gcccgacgtc ttcgcttgtc agcccgatca
     3481 acgagatcat tgaggagatc cgcgccggcc gcatggtgat cctggtcgac gaggaagatc
     3541 gcgagaatga aggcgatctg gtgctaggcg cccagttcac gaccccggcc cacatcaatt
     3601 tcatggccaa gcatgggcgc gggctgatct gcctcaccct gaccgaggat cgctgcaaac
     3661 agctgcggct accagccatg gtgcagaaga atggcgcacg tatgggcacc aacttcacca
     3721 tttccatcga agccgcagaa ggcgtgacca ccggcatctc ggcggcggac cgcgcccgca
     3781 ccatccaggc cgccgtggcc aggcaggcat cgcccaagga catcgtcagt cccggccatg
     3841 tgttcccact ggtcgccgag aacggcggcg tgctggtccg tgccggccac acagaagcag
     3901 gttgcgacct ggcttcgctg gcaggtctgg agccagc
//
LOCUS       DATMTW010000149         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_314629, whole genome shotgun sequence.
ACCESSION   DATMTW010000149 DATMTW010000000
VERSION     DATMTW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314629"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..977)
                     /gene="fmt"
                     /locus_tag="VN084_02770"
     CDS             complement(42..977)
                     /gene="fmt"
                     /locus_tag="VN084_02770"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWT28409.1"
                     /translation="MAALNVIFAGTPEFAVPALQALLDSRHVVCQVLTQPDRPAGRGM
                     KLKASPVKQLALQHGIPVLQPETLKDAAVQAQIAAAQADVMIVAAYGLIIPTVVLNMP
                     RLGCYNIHASLLPRWRGAAPIQRALLAGDRETGVTIMEVVPALDAGAMVRKGTLPIDE
                     RATAQSLHDGLAAIGGQLMVTAMDALADAGTLSAEPQDEALVTYAEKLQKSEAEIDWS
                     QPAAAISRQVRAFNPFPVAQSLLHGEVCRIWMATAQPGSGRPGEVVAIGDTLAVGTGE
                     GLLAIEVLQLPGGKRLAVREFMAGRKLKVGDRFGV"
     gene            complement(977..1477)
                     /gene="def"
                     /locus_tag="VN084_02775"
     CDS             complement(977..1477)
                     /gene="def"
                     /locus_tag="VN084_02775"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWT28410.1"
                     /translation="MAILDILNYPDPRLHTVAASVETVDDGIRRLVDDMAETMYAAPG
                     IGLAATQVNVHQQVIVIDTTEEKRDLRVFINPRIVSQRGRQQYEEGCLSVPGVYESVT
                     RAEQITVEALGRDGKPFTIEAEGLLAVCIQHEMDHLLGKVFVEYLSPLKQSRIKNKMK
                     KRQRER"
BASE COUNT          309 a          508 c          483 g          342 t
ORIGIN      
        1 gaatggcgga attcaatcgt cgacataccg ccgccgtaga ttcatacccc gaagcggtcg
       61 cctaccttga gcttgcgacc agccatgaac tctcgcaccg cgagtcgttt gccacccggc
      121 aattgcaaga cttcgatcgc aagcaagcct tcacctgtgc cgactgcgag ggtgtcgcca
      181 atcgcgacca cttcgccggg ccgcccgctg ccaggctgcg ccgtcgccat ccagatacga
      241 cagacctcgc catgcagcag gctctgcgct accgggaacg ggttgaatgc ccgcacctgg
      301 cgcgagatgg cagccgcagg ttggctccag tcgatctctg cctctgattt ctgcagtttt
      361 tccgcgtagg tcaccaaggc ttcgtcctgc ggttccgcag ataacgtgcc ggcatccgcc
      421 agggcgtcca tggccgtgac catgagctgg ccgccgatcg cagccagacc gtcatgcagg
      481 ctctgggcgg tggcgcgctc gtcgatcggc aatgtgcctt tgcgcaccat cgcgccggca
      541 tcgagcgctg gcaccacctc catgatggtc acccccgttt ctcgatcgcc ggccagcaat
      601 gcgcgttgaa tgggcgcggc gccgcgccat ctgggcagca gcgaggcgtg gatgttgtag
      661 cagccgagcc ggggcatgtt gagcacgacg gtcgggatga tcaagccata ggcagcgaca
      721 atcatgacat cggcctgggc ggcagcgatc tgcgcctgta ccgccgcatc cttcagggtc
      781 tcgggctgca ataccgggat gccgtgctgc aatgccagct gtttcaccgg gcttgccttg
      841 agcttcatgc cgcgacctgc cggccggtcc ggttgcgtca gtacctggca gaccacgtgc
      901 ctggaatcga gcagcgcctg caacgccggc acggcgaatt ccggcgtacc ggcgaagatc
      961 acgttgagcg ctgccatcaa cgttcgcgct ggcgcttctt catcttgttc ttgatgcggg
     1021 attgcttgag cggcgataga tattcgacga acaccttgcc cagcagatga tccatctcat
     1081 gctggatgca gacggcgagc aaaccttccg cttcgatcgt gaacggcttg ccgtcgcggc
     1141 cgagtgcttc gaccgtgatc tgctcggcgc gcgtcacgga ttcatagacg ccgggaacgg
     1201 aaagacaacc ctcttcatat tgctgcctgc cgcgctggga gacgatgcgt ggattgatga
     1261 acacccgcag gtcacgtttt tcctcggtgg tgtcgatcac gatcacctgc tgatggacgt
     1321 tcacttgcgt agcggccagg ccgatccctg gcgctgcgta catggtctcg gccatgtcgt
     1381 cgaccagccg acggatgccg tcatccacgg tttccaccga cgcggcgacc gtatgcagcc
     1441 gcgggtcagg ataattgagg atatctagga tcgccataaa ataagaaacg ccataaaagc
     1501 ttgattgttg aatgaattac atgcagaatt taatgcaact tcgcggatac taattctgcg
     1561 ccttgagacc cgaggtcata actatgtata aatacattat aacgccgcta ttattttgtt
     1621 gcctggcttt gccggcggtg gc
//
LOCUS       DATMTW010000150         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_415482, whole genome shotgun sequence.
ACCESSION   DATMTW010000150 DATMTW010000000
VERSION     DATMTW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415482"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /gene="pgk"
                     /locus_tag="VN084_02780"
     CDS             <1..355
                     /gene="pgk"
                     /locus_tag="VN084_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWT28411.1"
                     /translation="LKDADSVAADDMIFDIGPKSAKELADIIMNAATVVWNGPVGVFE
                     FDQFADGTKTIAEAIAKTKAFTLAGGGDTIAAIQKYDIYDRVSYISTAGGAFLEFLEG
                     KKLPAVDILEQRAQH"
     gene            419..1840
                     /gene="pyk"
                     /locus_tag="VN084_02785"
     CDS             419..1840
                     /gene="pyk"
                     /locus_tag="VN084_02785"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWT28412.1"
                     /translation="MSLRKTKIVATLGPASSSEEMIARMIVAGVDVVRLNFSHGSAED
                     HIQRAETVRRIALKLGRPVGVLCDLQGPKIRIGKFELGSVKLAVGDTFILDAACELGN
                     QQRVGLDYKALPQEVGPGSVLLLDDGRIVMAVEMVKGSEIHCTVTTGGILSNNKGINR
                     QGGGLSAAALTDKDKQDIKTAVALEADFVALSFPRSGADVEQARKLVRKAGGSASIVA
                     KIERAEAIDALEEIIAASDVIMVARGDLGVEVGDAAVPGLQKRMIRMARAQNKVVITA
                     TQMLESMINNPIPTRAEVSDVANAVLDGTDAVMLSAESAVGHYPLEAVSAMHRVCLEA
                     EKEYHGRQLPQKVNQEFQRTDEAIAMAAIYTAEHLKVKAIAALTQSGTVAQWLSRADT
                     LVPIYALSPLKQARRKLTLYRGVYPYPIDHAGKQRDEILQEMEDTLVRYGVVSKADLV
                     LFTFGEPIGTEGGTNTMKIVKIR"
BASE COUNT          398 a          541 c          579 g          336 t
ORIGIN      
        1 attgaaagac gcggatagcg tggccgctga cgacatgatc ttcgatattg gcccgaaatc
       61 tgctaaggaa ttggcagaca tcatcatgaa cgcggcgact gtcgtctgga atggcccagt
      121 tggcgtgttc gagttcgacc agttcgccga tggcaccaag accatcgccg aagccatcgc
      181 caagaccaag gccttcacct tggcgggcgg cggtgacacg atcgcggcca ttcagaagta
      241 tgacatttat gaccgggtat cctatatttc aactgcgggc ggcgcattcc tcgaattcct
      301 cgagggcaag aaactgccgg cagtcgatat cctggagcaa cgtgcccagc actagccgcc
      361 ttgcatggca tcaacaaaag gggctcgcca cgggcccctt tttattttcg ggagtcacat
      421 gagcttacgc aaaaccaaga tcgtcgctac cctcggcccg gcatcgagca gcgaggaaat
      481 gatcgcgcgc atgatcgtcg ccggcgtcga tgtggtcagg ctgaatttct cccatgggag
      541 tgccgaagat catatccagc gggccgagac cgtgcgcagg atcgccttga agctggggcg
      601 ccctgtcggc gtactgtgcg atctgcaggg gcccaagatc cgcatcggca agttcgaact
      661 ggggagtgtg aaactggcgg ttggcgatac cttcatcctc gatgccgcgt gcgaactggg
      721 caaccagcaa cgcgtcggcc ttgattacaa ggcgctgcca caggaggtcg gccccggcag
      781 cgtgctgctg cttgacgatg gtcgcatcgt tatggccgtg gaaatggtca agggcagcga
      841 gatccactgt accgtgacca ccggcggcat cctctccaac aacaagggca tcaaccgtca
      901 gggcggtggc ttgtctgccg cagcgttgac cgataaggac aagcaggaca tcaagacggc
      961 ggtggcgctg gaagccgatt tcgtcgccct gtcgttcccc cgcagcggtg ccgatgtaga
     1021 gcaggcgcgc aagctggtgc gcaaggcggg cggtagtgcc agcatcgtcg ccaagatcga
     1081 gcgcgccgag gccatcgacg cgctggagga gatcatcgct gcttccgatg tcatcatggt
     1141 ggcgcgtggc gacctcggtg tcgaagtcgg cgatgccgcc gtgcccggct tgcaaaaacg
     1201 catgatccgc atggcgcggg cgcagaacaa agtcgtgatc acggcgacgc agatgctgga
     1261 gtcgatgatc aacaacccga tcccgacacg ggcggaggtc tcggatgtgg ccaatgccgt
     1321 gctcgatggc accgatgccg tcatgctgtc tgcggagagc gcggtcggtc attatccgct
     1381 ggaggccgtc tcggccatgc accgggtctg tctcgaagcc gagaaggaat atcacggacg
     1441 ccagctgccg caaaaggtga accaggagtt ccagcgtacg gatgaagcca tcgccatggc
     1501 ggcgatctac acggccgaac acctcaaagt gaaagccatc gccgccctca cgcaatcggg
     1561 cacggtggcc caatggttat cgcgggccga tacgctggtg cctatatacg cgttgtcgcc
     1621 gctgaagcaa gcgcggcgca agctcacact ctatcgcggc gtctatccct atccgatcga
     1681 ccatgccggc aaacagcgcg acgagatcct gcaggagatg gaagatacac tggtgcgtta
     1741 tggggtcgtc agcaaggccg accttgtact cttcactttc ggcgagccga tcggcacgga
     1801 agggggcact aacaccatga agattgtcaa aataaggtaa gcatccgcat cgca
//
LOCUS       DATMTW010000151         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_214667, whole genome shotgun sequence.
ACCESSION   DATMTW010000151 DATMTW010000000
VERSION     DATMTW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214667"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VN084_02790"
     CDS             <1..243
                     /locus_tag="VN084_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28413.1"
                     /translation="VITLQNIDNPLSLFETTAHKLQASGYLRISQTPYIQTGGISARK
                     KKAARKPTRCPYTLDFLEDRADSERPQPLTPPDLFT"
     gene            complement(292..1623)
                     /gene="rimO"
                     /locus_tag="VN084_02795"
     CDS             complement(292..1623)
                     /gene="rimO"
                     /locus_tag="VN084_02795"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWT28414.1"
                     /translation="MMLKSVPKVGFVSLGCPKASSDSERILTQLRAEGYEISGSYEDS
                     DLVVVNTCGFIYSAVAESLDAIGEALDKNGKVIVRGCLGAKADVVQNAHPSVLAVTGP
                     HAMEEVMAHVHGHLPKPHDPYTDLVPPQGIRLTPKHYAYLKISEGCNHRCSFCIIPSM
                     RGDLVSRPIGDVMQEAENLVNAGVSELLVISQDTSAYGVDVKYRTGFWNGRPIKTRMT
                     ELAKGLGELGVWVRMHYVYTYSHVDEIIPLMADGLILPYLDVPFQHASPRILKAMKRP
                     ASSENNLARINAWRDICPDITIRSTFIVGFPGETDEDFEMLLDFMQEAQLDRVGCFAY
                     SSVDGAVANALPNHVPEALKQERLARFMAVQEEISANKQRNKIGRIEMVLVDEVEGDV
                     AIGRTSADAPEIDGVVHLADADGLMPGDLVEVEITGSDGHDLWAAPPSREE"
     gene            1635..1871
                     /locus_tag="VN084_02800"
     CDS             1635..1871
                     /locus_tag="VN084_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28415.1"
                     /translation="MYIVAIAWLYVTLMMAVTEHSLAAGILTFLFYGLLPCALFVWLF
                     STPQRRRNREAREAEAARAASVEERLDQPDRADP"
     gene            complement(1827..2612)
                     /locus_tag="VN084_02805"
     CDS             complement(1827..2612)
                     /locus_tag="VN084_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWT28416.1"
                     /translation="MSTLSWIITTSLLGGVLSVLAAGFMAFKARRSWVPMLISYAIGA
                     LLGAVFLEILPHAFHASGSIDAMAVTVLLGILLFFILEKLVLWRHCHGDECEVHAGHD
                     AHQAGDDHHDHGRSGLMIMVGDTFHNFVDGILIAAAFMIDTNLGVVTALAIIAHEIPQ
                     EVGDFLILLHSGYSKRQALLFNLLSSLATLVGGLLGYFALQSVQTWIPTILGLAAASM
                     IYVAVADLIPGLHKRTELKATIQQVVLIALGISSIWLVKALLD"
     gene            complement(2691..3446)
                     /gene="pgeF"
                     /locus_tag="VN084_02810"
     CDS             complement(2691..3446)
                     /gene="pgeF"
                     /locus_tag="VN084_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWT28417.1"
                     /translation="MTTTLNEAGLIRPDWPAPDNVRALQTTRAGGISLAPYASMNLGD
                     HVADDPLHVAANRQRLQPWLPSEPVWMRQVHGTAVVCADQASCLPEADAAYARRPNTV
                     CTVMTADCLPLLLCDRAGSVVAAVHAGWRGLLAGVIEATIRDMAVAPGELMAWLGPAI
                     GPEAFEVGAEVRQMFVDKDAAATQAFASTVNGKWLADIYQLARGRLRQSGVNAVYGGQ
                     YCTYRDDERFFSYRRDGVTGRMASMVWLVGEHV"
     gene            complement(3427..>4172)
                     /locus_tag="VN084_02815"
     CDS             complement(3427..>4172)
                     /locus_tag="VN084_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWT28418.1"
                     /translation="HPAAGNWEGTLLNGLLYHLPALERVPRAGIVHRLDKDTSGLLVV
                     AKHLEAQTRLVRQLQARTVKREYRAIVWGQIWRNGTADQPIGRHPHARTKMAVNRMGK
                     PAVTRYEVLERFGVHTYLRCLLETGRTHQIRVHMQHLQAPIVGDPVYGIRGIIPHRFM
                     TDVLFDAVQGFKRQALHAIRLGLTHPASGLPMEWQIDLPADMKLLLEAMRHEDPVTDE
                     TFSFEMLEDADDLDDADFDDETLDDDDIE"
BASE COUNT          877 a         1304 c         1183 g          808 t
ORIGIN      
        1 gtcatcacgt tacagaatat cgacaatccg ctgagcctgt tcgagaccac agcgcataaa
       61 ctgcaagcat ccggctacct ccgcatcagc cagacccctt acatccagac cggtggcatc
      121 agcgccagaa agaaaaaggc agcgcgcaaa cctacacgct gcccttacac actcgacttc
      181 ctggaagacc gggctgattc cgaacgtccg caaccactga caccaccaga tttattcacc
      241 tagtgcggct ggcatgaact gacaggctgc tcctgtcgca caaccgagga ctcactcttc
      301 cctgctgggt ggtgccgccc acaaatcgtg accatccgaa cccgtgatct ccacctcgac
      361 cagatcgcca ggcatcaggc catcggcatc tgccagatgc accacgccat cgatctctgg
      421 agcatcggcg ctggtgcgac caatggcgac gtcaccttcg acctcgtcga ccagcaccat
      481 ctcgatacgc ccgatcttgt tgcgttgctt gttggcgctg atctcctcct gcaccgccat
      541 gaaccgtgcc agccgctcct gcttcaacgc ttccgggaca tggttcggca aagcgttggc
      601 gacggcgcca tcgactgatg aataggcgaa gcaccccaca cggtcgagct gcgcctcctg
      661 catgaagtcg aggagcatct cgaaatcttc atcggtttca cccgggaagc cgacgatgaa
      721 cgtactgcgg atggtgatat ccgggcaaat atcccgccag gcattgatcc gagccaggtt
      781 gttctcgctg ctggccgggc gcttcatggc cttgaggata cgcggactgg catgctggaa
      841 cggtacatcc agatacggca ggatgaggcc atccgccatc agcgggatga tctcgtcgac
      901 atgcgagtat gtatagacat aatgcatgcg cacccagacg cccagttcac cgagcccttt
      961 cgccagctct gtcatgcgcg tcttgatcgg ccgcccattc cagaagccgg tgcgatattt
     1021 gacatcgacg ccataagcgc tcgtgtcctg cgagatcacc agcagctctg aaacgccggc
     1081 attgaccagg ttctctgcct cttgcatgac atcgccgatc ggccggctga caaggtcgcc
     1141 acgcatggag gggatgatgc agaaactaca gcgatggtta cagccctcgg atatcttgag
     1201 ataagcgtag tgcttgggtg tcaggcgaat gccttgcggc ggcaccagat cggtatatgg
     1261 gtcatgcggt ttaggcaagt ggccatggac atgcgccatg acctcttcca tcgcatgtgg
     1321 cccggtcacc gcgagcacac tcggatgtgc gttttgcacc acgtcagcct tcgcgcccag
     1381 acaacctctg acgatgacct tgccgttctt gtcgagcgct tcgccgatcg cgtcaagcga
     1441 ttccgcgacg gcgctataga tgaagccgca ggtattcacc acaaccagat cggaatcctc
     1501 ataggaaccc gatatttcgt agccttccgc gcgcaattgc gtgagaatac gttccgaatc
     1561 tgacgaagcc ttggggcaac ccagagaaac gaaaccgact ttaggcactg atttaagcat
     1621 cattattgac atccatgtat atcgttgcaa tcgcgtggct ctacgtcacg ctcatgatgg
     1681 ccgtgactga acactcgctg gccgcaggta tcctgacttt cctgttctac ggactgttgc
     1741 cctgcgcgtt attcgtttgg ctcttttcga ccccgcagcg acgccgcaat cgggaagcac
     1801 gcgaggccga agccgccaga gccgcctcag tcgaggagcg ccttgaccag ccagatcgag
     1861 ctgatcccta gcgcgatcaa tactacctgc tgaatggtcg ctttcaattc cgtacgcttg
     1921 tgcaggccgg gaatcaggtc agcgaccgcc acatagatca tgcttgctgc cgccagcccg
     1981 aggatggtcg ggatccaggt ctggacgctc tgcaaggcaa aatagccgag caggccgccg
     2041 accagtgtcg cgaggctgga taagaggttg aacagcagcg cctgccgctt gctgtagccg
     2101 gaatgcagca ggatgagaaa atcgccgacc tcctgcggga tctcgtgggc aatgatggca
     2161 agcgccgtga ccacgccaag gttggtatcg atcatgaagg ccgccgcgat caggatgccg
     2221 tcgacgaagt tatggaaggt atcgccgacc atgatcatga gtccgctgcg gccatggtcg
     2281 tgatgatcat cgccagcctg gtgggcatcg tggccggcgt gcacctcaca ttcgtcgcca
     2341 tgacaatgcc gccagagcac caacttttcc aaaatgaaaa acagcaggat gccgagcagt
     2401 accgtcacgg ccatggcatc gatgctgccc gatgcatgga aagcatgcgg caatatctcg
     2461 agaaaaacgg cgccgagcaa tgcgccgatg gcatagctga tcagcatggg gacccagcta
     2521 cggcgtgcct tgaacgccat gaagccggcc gccagcacac tcaggacgcc gcccagcagg
     2581 ctggtcgtga tgatccagga aagcgtagac atatcgggaa agggcatcaa ccccggcaaa
     2641 tttgaagaat cggcattata aacgaggggc cggcttaccg cgcttgaatt tcagacatgc
     2701 tcgccgacga gccagaccat gctggccatg cgcccggtga cgccatcgcg ccgatatgag
     2761 aagaaacgct cgtcatcccg gtaagtgcaa tattgaccgc cgtagacggc gttgacccct
     2821 gactggcgta agcggcctct cgccaattgg tagatatcgg caagccattt gccgtttact
     2881 gtcgacgcga atgcctgtgt cgcggcagca tccttgtcga cgaacatctg cctgacctct
     2941 gccccgacct cgaatgcttc ggggccgatc gccgggccta gccaggccat gagttcccca
     3001 ggcgcgactg ccatgtcgcg gatcgttgcc tcgatgacac cggccagcag cccgcgccat
     3061 ccagcatgca cagcggcaac gacgctaccg gcacggtcgc acaacaaaag cggcaggcaa
     3121 tccgccgtca tcaccgtgca tacggtattc ggccggcgag cgtaggctgc atccgcttcc
     3181 ggcaggcaac tcgcctggtc ggcgcacacc accgcggtgc cgtgcacttg ccgcatccag
     3241 accggctcgc taggtaacca tggctggaga cgctgtcggt tggcggccac gtgcaaggga
     3301 tcatccgcga catggtcgcc gaggttcatg ctggcatacg gtgccagact gatgccgccg
     3361 gcgcgcgtag tctgcaacgc ccgaacattg tcgggcgccg gccagtcggg cctgatgagg
     3421 ccagcttcat tcaatgtcgt cgtcatccag cgtctcgtcg tcgaagtctg catcgtcgag
     3481 atcatccgca tcctccagca tctcgaagct gaatgtctcg tcggtcactg ggtcctcgtg
     3541 tcgcatggct tcgagcagta acttcatgtc cgctggaagg tcgatctgcc actccatcgg
     3601 caagccgctc gcggggtggg tcaaccccag gcgaatggcg tgcaaggcct ggcgcttgaa
     3661 gccttgtacc gcatcgaata acacgtccgt catgaaccga tgcgggatga tgccgcggat
     3721 gccatagacc ggatcaccca ctatcggcgc ctgcaaatgt tgcatatgga cacggatctg
     3781 atgggttcgg ccagtctcca gcaagcatcg caggtaggta tgcaccccga agcgttccag
     3841 cacctcataa cgcgtcaccg caggcttgcc catgcggttg accgccatct tggtcctggc
     3901 atgcgggtga cgtccgatcg gctggtcggc cgtaccgttg cgccagatct ggccccagac
     3961 gatcgcgcga tattcgcgtt tgacagttct cgcctgcaat tgccgcacaa ggcgggtctg
     4021 ggcttcaaga tgcttggcga caaccagcag gccgctggtg tccttgtcca gtcgatggac
     4081 gatgcccgcg cgcggcacgc gctccagggc aggcagatgg tacaacaagc cgttgagcaa
     4141 agtgccttcc caattccctg cagctggatg ca
//
LOCUS       DATMTW010000152         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_247666, whole genome shotgun sequence.
ACCESSION   DATMTW010000152 DATMTW010000000
VERSION     DATMTW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247666"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VN084_02820"
     CDS             complement(<1..543)
                     /locus_tag="VN084_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWT28419.1"
                     /translation="MIESMIRAALKQRLVIVVIALALMVAGVFAMKKLSVDAFPDVTN
                     IQVLIATQSTGRSPEEMERFITVPLEIAMTGLPGLTEMRSLNKNGLSLITLVFTDNTD
                     VYFARQLVMERLMEVMDRMPEGVTPVLGPVSTGLGEVYQYTLDKPTDGDKELTQADLT
                     ERRAIQDWIVRPLLRGILGVA"
     gene            complement(547..1722)
                     /locus_tag="VN084_02825"
     CDS             complement(547..1722)
                     /locus_tag="VN084_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWT28420.1"
                     /translation="MSFLIDTYNPATCSTRAVVSLLLALTLAGCGKTEVEAPATTQVT
                     DLNQVELTPELQQQIKLHKVGESEIREVLRIPGSIHVDEQRVARMGAPVTGRITDIDV
                     VLGQDVKEGQLLATLNSTELAQNQLTYIKAQQQIALQTKAVERAKLLLEADVIGAAEL
                     QRREAELSAAHAELNAARDQLQILGMSDNAITQLSKSSSIRSYSSVSARLAGTVISRN
                     VNLGQVVQPAEELFIVADLSHVWAVAEVPEQQIELIQEGEEVSIEVPALSDREFTGRL
                     IYVGDVVNPKTRTVTVRTDLKNTEGDIKPDMLVSMLVQSKPSSRIVVPAQAIVRENDR
                     DHVFVQMAPNKYRLREVVLGPEYHGKVAVLSGLDIGETVVGEGAFHVNNERKRKELE"
     gene            complement(1722..>2121)
                     /locus_tag="VN084_02830"
     CDS             complement(1722..>2121)
                     /locus_tag="VN084_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWT28421.1"
                     /translation="SFHLGVAVPLPLWSQRQGQIAEAAAGAREVRSILSERELALRRD
                     LDSSYQRYLIARQQVSSFENGLLSQSEAVLKTAEAAYRYGERGILEYLDAQRTFRVVR
                     KDYLTARYDYVNAMLEIERVLGTEILEDKP"
BASE COUNT          379 a          652 c          655 g          435 t
ORIGIN      
        1 ggcgacgccg aggatgccgc gcagcaaggg tcgcacgatc cagtcctgga tggcgcgacg
       61 ctcggtgaga tcggcctggg tcaattcctt gtcgccgtcg gtgggcttat cgagcgtgta
      121 ctggtagacc tcgcccaagc ccgtggacac cggaccgagc acaggcgtga caccttccgg
      181 catgcggtcc atgacttcca tcaggcgctc catgaccagc tggcgcgcga aatagacatc
      241 ggtattgtcg gtgaacacaa gcgtgatcag ggacagtccg ttcttgttca gggaacgcat
      301 ctcggtcaga ccgggcaggc cggtcatggc gatctcgagc ggcacggtga tgaagcgttc
      361 catctcttcc ggcgaacgac cggtggattg ggtggcgatc aacacctgga tgttggtgac
      421 atcggggaag gcgtcgacgg agagtttctt catcgcgaac acgccagcca ccatcagggc
      481 tagcgcgatc acgacgatga cgagacgctg cttgagcgca gcgcggatca tggattcgat
      541 catggcttat tccagttctt tgcgcttgcg ctcgttattt acatggaatg cgccctcgcc
      601 caccactgtc tcgccgatat cgagtccact cagcaccgcg accttgccgt gatactcggg
      661 cccgagcacg acttcgcgca gacgatactt gttcggcgcc atctggacga agacatggtc
      721 acggtcgttc tcgcgaacga tcgcctgtgc aggcacgacg atacgggaag acggtttgga
      781 ctggaccagc atggacacca gcatgtccgg cttgatgtcc ccctcggtgt tcttgagatc
      841 ggtgcgcacc gtcactgtcc gcgtcttggg gttcaccacg tcgccgacat agatgagtcg
      901 gccagtgaac tcgcggtcac tcagcgccgg cacttcgatg ctcacttcct cgccctcctg
      961 gatcagctcg atctgctgct ccggcacttc ggccactgcc cagacgtgcg agagatcggc
     1021 cacgatgaaa agctcctcgg ccggctgcac cacctgaccc agattgacgt tgcggctgat
     1081 gaccgtgcct gccagtcttg ccgaaacgct gctgtaggag cggatcgagc tcgacttgga
     1141 gagctgtgtg atcgcattgt cggacatgcc caggatctgc agctggtcac gcgcggcatt
     1201 gagttcggca tgcgccgcgc tcagttccgc ctcgcggcgt tgcagttcgg cggcgccgat
     1261 gacgtcagcc tcaagcagca atttggcacg ctcgaccgcc ttggtctgca acgcgatctg
     1321 ctgctgcgcc ttgatatagg tgagctggtt ctgtgcgagc tcggtgctgt tgagcgtggc
     1381 cagcaactgg ccctccttca cgtcctgccc gagcacgaca tcgatatccg tgatgcggcc
     1441 agtgactggc gcgcccatgc gggcgacgcg ctgttcatcg acatggatgc tgcctgggat
     1501 gcgcaacact tcgcggatct cggattcacc aaccttgtgc agcttgatct gctgctgcag
     1561 ttcgggcgtc agttcgacct ggttgagatc cgtcacctgc gtggtggcag gcgcttcgac
     1621 ctcggtcttg ccgcaaccgg cgagcgtcag ggccagtagg agactgacca ctgcccgtgt
     1681 ggagcaggtg gcgggattgt aggtatcgat cagaaaagac atcagggctt atcctccagg
     1741 atttcggttc cgagcacacg ctcgatttcc agcatcgcat tcacatagtc atagcgtgcc
     1801 gtcaggtaat ctttgcgcac tacgcggaaa gtccgctgtg cgtccaggta ttccagtatg
     1861 ccgcgctcgc catagcgata tgcggcttcc gccgttttca ggacggcctc agactggctc
     1921 agcagcccat tctcgaagga ggacacctgc tggcgcgcga tcaggtagcg ctggtaggac
     1981 gagtccaggt cgcggcgcag cgccagttcg cgctcgctga ggatcgaccg gacctcacgc
     2041 gcgccagcgg cggcttcggc gatctgccct tgccgctggc tccataacgg cagtggcaca
     2101 gccacgccca gatggaaact g
//
LOCUS       DATMTW010000153         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_214689, whole genome shotgun sequence.
ACCESSION   DATMTW010000153 DATMTW010000000
VERSION     DATMTW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214689"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VN084_02835"
     CDS             <1..523
                     /locus_tag="VN084_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28422.1"
                     /translation="EMKKLQIAHHPGNGYLMVDLDIDSFFEGGETTENPFLALFKRHA
                     WSEHELVVNVVENHTTADALRSQRLIELLQRSGTAVALEDVGVGWGMFSLSAFLDASV
                     VKFNGMALKQLNEQAAEATLDWLVSAARRIGVQTILDGVSSCDDMDWARRMGVDCVQG
                     ALFARQAVMARSG"
     gene            655..1128
                     /locus_tag="VN084_02840"
     CDS             655..1128
                     /locus_tag="VN084_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWT28423.1"
                     /translation="MAKDKKADSGINTPVIKALNTILEMELAGVVRYTHYALMIYGYN
                     RIPIVSWLRGQATESLDHANKAGELITHLGGHPSLSIGPLLETHNHDVGDILRESLDH
                     ENASLNCYRKLLKLVEGKSVMLEEYAREMIYTEELHLGEVNKMLRKPGEVGVYHD"
     gene            complement(1201..1737)
                     /locus_tag="VN084_02845"
     CDS             complement(1201..1737)
                     /locus_tag="VN084_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HWT28424.1"
                     /translation="MRIAVQLSALSLLLACLTAQAADQPADGQPVPIAAEDGGWSGPA
                     QEILINALSLTGIKYKYGGISPETGFDCSGFVRYVFQQAANLTLPHGARALSQLGQTV
                     PLDQLRPGDLVFFNTLKSAFSHVGIYLGDNRFIHAPSSGGGIHVVNMNDAYWSERFNG
                     ARRIEVAAPPSEAQKPAP"
BASE COUNT          442 a          553 c          617 g          373 t
ORIGIN      
        1 cgagatgaaa aagctgcaga tcgcacatca tccgggcaat ggctacctca tggtcgatct
       61 cgatatcgac agcttcttcg aaggtggcga aaccacggag aacccgttcc tggcactttt
      121 caagcgtcac gcatggtcgg agcatgagct tgtcgtcaac gtcgtggaga accacaccac
      181 agctgacgcc ttgcgctcgc aacgcctgat cgaactcctg caacgcagcg gcacggcagt
      241 ggcgctggaa gatgtcggtg tgggctgggg catgttttcc ctgagcgcat tcctcgacgc
      301 cagcgtcgtc aagttcaacg gcatggcctt gaagcagctc aacgaacagg ccgccgaagc
      361 caccctcgac tggctggtga gcgcggcgcg gcggatcggc gtgcagacca tcctggacgg
      421 cgtcagcagc tgtgacgata tggattgggc gcgccgcatg ggggtcgatt gcgtgcaggg
      481 cgcactcttc gcgcgacaag cggtcatggc gcgcagcgga tgaccgtccg ccggcaacgc
      541 cgcgagttgc gattcacaat gtgaactatt cgcaattctg cacgaactgc cagcctggga
      601 tagactgccg ccatggctgc aaggcccgat ccaaaaacac cttaggaggt caccatggcg
      661 aaagacaaaa aagccgatag cggcatcaac acccccgtca tcaaggcgct gaacactatc
      721 ctggagatgg aactggctgg cgtggtgcgt tacacgcact acgcactcat gatctatggc
      781 tataaccgta tcccaatcgt ctcctggcta cgcggacagg cgaccgaatc gctcgatcat
      841 gccaacaagg ccggcgagct gatcacccat ctgggtgggc atccgtcgct gtcgatcggc
      901 ccgctgctcg aaacgcataa ccatgatgtg ggcgatatcc tgcgcgaatc gctggatcac
      961 gagaatgcct cgctcaactg ctaccgcaag ctgttgaagc tggtggaagg caagtcggtg
     1021 atgctggagg aatatgcgcg cgaaatgatc tacaccgagg aactgcatct gggtgaggtc
     1081 aataagatgt tgcgcaagcc tggcgaagtc ggtgtttatc acgactgaac gcatctgacg
     1141 caaacgtagg caaaacgaga acggccggct aaaccccggc cgtttttcat gcggctggat
     1201 tcatggcgcc ggcttctgcg cctctgaagg tggcgcggcg acttcgatgc gtcgtgcgcc
     1261 gttgaagcgt tcgctccagt aggcgtcgtt catgttgacg acgtggatgc cgccgccgga
     1321 gctaggcgca tggatgaagc ggttgtcgcc gagatagata ccgacgtggg agaacgccga
     1381 cttcagcgta ttgaagaaca cgaggtcgcc cgggcgcagc tggtcgagcg gcacggtctg
     1441 gccgagctgg ctcagcgcgc gtgcgccgtg tggcaaggtg aggttggcag cctgctggaa
     1501 aacatagcgc acgaagccgc tgcaatcgaa acctgtttca ggtgagatgc cgccatattt
     1561 gtatttgatg ccggtgaggc tgagggcatt gatgagaatc tcctgcgctg gaccggacca
     1621 gccgccatct tcggccgcga tcggcacggg ctggccatcg gcgggctgat ccgcggcttg
     1681 cgccgtgagg caggcaagca acaggctcag agcggaaagt tggacagcga tgcgcatgcc
     1741 ggcattctaa aaggcacatg cggcatttgt aaacctttga cttatttgca taaacgatgg
     1801 cttgccaaac cgcatgagtg agatggtata gaattgggct gaagcaaccg atcagcagga
     1861 gtgtatcgat gtcagctcat cgcgtgattt ccatggcagg acacaaggcg agtaccacga
     1921 cccatcaggc gcaactgtca ccaaggactg ggccgcgtcg acaaatccag gaaacatggt
     1981 tcgat
//
LOCUS       DATMTW010000154         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_281008, whole genome shotgun sequence.
ACCESSION   DATMTW010000154 DATMTW010000000
VERSION     DATMTW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281008"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VN084_02850"
     CDS             complement(<1..347)
                     /locus_tag="VN084_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWT28425.1"
                     /translation="MSVGNAIRKRRKQLNLTLQELAGRVDTDSGNLSRIERGTQGVSE
                     AMLMRLCDALDCTAAYLYSQTESASGTSTPASPRLNLLQPQEFVRWFRAAAPYIHAFG
                     GRTFVIAFGGEVV"
     gene            complement(423..1160)
                     /locus_tag="VN084_02855"
     CDS             complement(423..1160)
                     /locus_tag="VN084_02855"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWT28426.1"
                     /translation="MPNPRFYHPEKLAPGATITLTPNAAVHASRALRLQVGDQALLFN
                     GDGNDYLCELIVVKKTEVVAKIKAVEHVDSESPLKISLAQAISSGDRMDYTIQKAVEL
                     GVGAIQPIASQRSVVKLTGERAEKRREHWQNVVISACEQSGRAVLPTVAAPLSLADWL
                     ARPSSADLRITLSPTAEHCLHELPPPQGEICLLIGAEGGLTEHEIALAASQGFVPVRL
                     GNRILRTETAPIAAISAMQTLWGDFNR"
     gene            1223..1789
                     /locus_tag="VN084_02860"
     CDS             1223..1789
                     /locus_tag="VN084_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWT28427.1"
                     /translation="MASVNPPVCDFGWIAPKFRLPGVDGRTWSLRDAAGPKGTLVMFI
                     CNHCPYVKAIRPRLVADLRELQTLGINAIAIQSNDVIAYPEDNFEAMKAVSAEFNFPF
                     PYVLDEAQEVAKAYGAVCTPDFFGFNANFELQYRGRFDESRKETAEGSTRDLFYAMKM
                     IAETGEGPREQIASIGCSIKWLEERAAG"
     gene            1988..>3428
                     /gene="tkt"
                     /locus_tag="VN084_02865"
     CDS             1988..>3428
                     /gene="tkt"
                     /locus_tag="VN084_02865"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWT28428.1"
                     /translation="MATRTDLCNAIRALSMDAVQKANSGHPGAPMGMAEIAEVLWNHN
                     LSHNPKNPNWANRDRFVLSNGHGSMLIYSLLHLTGYDVTIDDIKTFRQLHSKCAGHPE
                     YHYAPGVETTTGPLGQGIANGVGFAMAEKLLANQFNKPGHDIVDHYTYVFLGDGCMME
                     GVSHEACALAGTWGLGKLMAFWDDNGISIDGHIEGWYTDDTAKRFEAYGWHVQSVDGH
                     DSAAIQKAIDAAKKVTDKPSLICCKTIIGKGSPNKCGSHDCHGSALGEDEVAATRAAI
                     GWPHEPFVVPADVYEGWDAKAKGAKAEADWNAKFDAYAKAYPAEAAEFKRRMAGELPA
                     NWKQVTDAMIAATNEKAEGVASRKASQNVIGALAPVLPEFLGGSADLTGSNLTSTGSF
                     KHVSGKEPGNYISYGVREFGMAAIMNGMALHGGLLPFGGTFHMFSDYMKNGMRMSALM
                     KQRVVYVLTHDSIGQGEDGPTHQPVENTSG"
BASE COUNT          731 a         1014 c         1017 g          666 t
ORIGIN      
        1 tcgaccacct cgccaccgaa agcgatgacg aaggtgcgtc cgccgaaggc gtggatataa
       61 ggcgcggcgg cgcggaacca gcgaacgaat tcctgcggct gcaatagatt gaggcgcggg
      121 ctggcgggcg tcgaggtgcc ggaagcgctc tccgtctgag aatagaggta ggcggcggtg
      181 caatcgagtg cgtcgcacaa gcgcatgagc atggcctccg acaccccttg cgtgccacgt
      241 tcgatacgcg acaggttgcc gctgtcagta tcgacacggc ctgcgagttc ctgcagggtc
      301 aggttgagtt gcttgcgccg cttgcggatg gcattaccga cagacatgac taggccttta
      361 atttgctata tacgcacata tttatacatt attgaggaat ttgcaacaaa tatgcgcaga
      421 atctagcgat taaagtcccc ccataacgtt tgcattgcgg atattgctgc aatgggcgca
      481 gtttctgtgc gcaagatgcg gttgcccaga cgcaccggta cgaatccctg gctggcggca
      541 agcgcgatct cgtgctcggt caggccgcct tcggcaccaa tcagcaggca gatctcgcct
      601 tgtggtggag gcaattcgtg caggcagtgc tcggccgtcg gcgagagcgt gatgcgcagg
      661 tcggcgctgg agggccttgc cagccagtcc gccagcgaga gtggcgccgc tacggtggga
      721 agtaccgcgc gccccgattg ctcacaggcc gagatgacga cgttctgcca atgctcacgc
      781 cgcttttcgg cccgctcgcc cgtgagcttg accacgctgc gctggctggc aataggctga
      841 atggcgccta cccccagttc caccgctttc tgtatggtgt aatccatacg gtcaccgctg
      901 gagatggcct gggccagcga gatcttgagt ggggattcgc tatccacgtg ttccacggct
      961 ttgatcttgg ccacgacctc ggttttcttg accacgatca gttcgcacag gtaatcgttg
     1021 ccgtcgccat tgaacagcag ggcctggtca cccacttgca gacgcagggc gcggctggcg
     1081 tggacggctg cattgggcgt tagcgtgatg gtggcgccgg gcgccagttt ttccggatga
     1141 tagaagcgcg ggttgggcat cgtcgggcgg ccatgtagaa tttgtctgaa cgacgattct
     1201 aattgattga aggagtgatg tgatggcaag tgttaatccg cctgtttgtg attttggctg
     1261 gatcgcgccc aagttccgct tacccggagt ggatggtcgc acctggtcgc tgcgtgatgc
     1321 agccggcccc aagggtacgc tggtgatgtt catctgcaat cattgcccct acgtcaaggc
     1381 gatccgccca cgcctggtcg ccgatctgcg cgaactgcag acccttggca tcaatgccat
     1441 cgccatccag tccaacgatg tgatcgcgta cccggaagac aatttcgagg ccatgaaggc
     1501 ggtctcggcc gaattcaatt tccctttccc gtatgtgctg gatgaggcgc aagaagtggc
     1561 caaagcctat ggcgcagtct gcacgccaga ctttttcggc ttcaatgcca atttcgaatt
     1621 gcagtatcgc ggccgcttcg acgaatcgcg caaggagact gccgaaggga gtacgcgtga
     1681 cctgttctac gccatgaaaa tgatcgccga aacgggcgag gggccacgcg aacagatcgc
     1741 cagtatcggc tgctcgatca agtggctgga agaacgggct gcaggctgag cacggcttgc
     1801 tgaaaaccgc gatctaagca ataattgtcg tctatacccc tgataaaaac aaagacttgg
     1861 cgttgagcaa aatcctgtaa cagcaggggg attcctgaag aacaagaggc gaggctggtt
     1921 cctggaccat gggtcgggaa gctgcctttt tctctattaa acccggtttg aaagatggga
     1981 gattaaaatg gcaactcgta ccgacctatg taatgccatc cgtgcactgt ccatggatgc
     2041 ggtccagaag gcaaattctg gccacccagg cgccccgatg gggatggcag aaattgccga
     2101 agtgttgtgg aaccacaacc tgagccacaa cccgaagaac cctaattggg ctaaccgcga
     2161 ccgtttcgtg ctgtccaacg gtcacggctc catgctgatc tactccctgc tgcacctgac
     2221 cggctatgac gtcaccatcg acgacatcaa gaccttccgt caactgcact ccaagtgcgc
     2281 aggccaccct gaataccact acgcgccagg cgttgaaacc accaccggcc cactgggcca
     2341 gggtatcgcc aacggcgtcg gtttcgccat ggccgagaag ctgctggcca accagttcaa
     2401 caagccaggt catgacatcg tcgaccacta cacctacgtg ttcctgggtg acggctgtat
     2461 gatggaaggc gtttcccacg aagcctgtgc actggccggt acctggggcc tgggcaagct
     2521 gatggcgttc tgggacgaca acggcatctc catcgacggc cacatcgaag gctggtacac
     2581 cgatgacacc gccaagcgtt tcgaagccta cggctggcac gtccagtccg tcgacggcca
     2641 cgattccgca gcgatccaga aagccatcga cgctgccaag aaggtcaccg acaagccatc
     2701 cctgatctgc tgcaagacca ttatcggtaa gggctccccg aacaagtgcg gttcgcacga
     2761 ctgccacggt tccgcactgg gcgaagacga agttgccgcc acccgtgcag cgatcggctg
     2821 gccacacgag ccgttcgtcg taccggccga cgtttacgaa ggctgggatg ccaaagccaa
     2881 gggtgccaag gctgaagctg actggaacgc caagttcgac gcatacgcca aggcttaccc
     2941 agctgaagcc gctgaattca agcgccgcat ggccggcgaa ctgccagcca actggaagca
     3001 agtgaccgat gccatgatcg cagcgaccaa cgaaaaggcc gaaggcgtcg ccagccgcaa
     3061 ggcatcgcag aacgtcatcg gcgcactggc gccagtcctg ccggaattcc tcggcggttc
     3121 cgccgacctg accggttcca acctgaccag cacgggcagc ttcaagcatg tcagcggcaa
     3181 ggagccaggc aactacatct cctacggtgt tcgcgagttc ggcatggccg cgatcatgaa
     3241 cggtatggcg ctgcacggcg gtctgctgcc gtttggcggt actttccaca tgttctccga
     3301 ctacatgaag aacggcatgc gcatgtccgc gctgatgaag caacgcgtgg tctatgtgct
     3361 gacccacgac tccatcggcc agggcgaaga cggcccgacc caccagccag tcgagaacac
     3421 ctccggcc
//
LOCUS       DATMTW010000155         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_114326, whole genome shotgun sequence.
ACCESSION   DATMTW010000155 DATMTW010000000
VERSION     DATMTW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114326"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VN084_02870"
     CDS             complement(<1..837)
                     /locus_tag="VN084_02870"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWT28429.1"
                     /translation="MHKEHFGVLDGWRGISILLVLAAHLLPLGPKAWDLNVAAGLLGM
                     VLFFNLSGFLITHFLLQRPSVPDFLMRRFFRILPVAWLYMGLMLYLTPASGATWSAHF
                     LFYANYPPEPLIFTTSHLWSLCVEVHFYLGIALLVGFFGKRGLLAIPLLCVAFTLLRV
                     SDGIHYAVISHYRVDEILAGAWLALIYNSRPDAMLRRFVSQGSLLVLVPLLLVACHPD
                     SGAFNYLRPYLAAALIGATMLDKTSPLVPLLQQRLLVYIAGISYALYVIHPFLMSTWL
                     GSG"
     gene            complement(864..1928)
                     /locus_tag="VN084_02875"
     CDS             complement(864..1928)
                     /locus_tag="VN084_02875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWT28430.1"
                     /translation="MFVWVQVLRGLAASLVVLHHYLASQIERGAEVSHWLENLGASGV
                     DIFFVISGFIMMITTSQMTRTPSAGDFLKRRLVRIAPLYWTLTAIAFAMVLAAGSAVN
                     TVVSPQKFVASMLFLPYHDGAIDMGMLAHKAYVIPMAWTLTYEWYFYLVFALTLAIGL
                     RPAGRLMFMTAWFAVTVGVGMLLQPTALIAQVVSQPMVFEFLLGCVIAMLYSRGLRIG
                     GGAALLLAGSGLFVLGQIFHHTAYDRTLYWGTAAFMLIAAATLYRGSQSETPALRPFS
                     WFGDISYSLYLSHFFTLALFVRLQRRFDLLADSFAPWTLIAFLLLVTMVAAACYYGIE
                     EPARKRFARRKRPLGAALNT"
     gene            complement(1944..>2287)
                     /locus_tag="VN084_02880"
     CDS             complement(1944..>2287)
                     /locus_tag="VN084_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWT28431.1"
                     /translation="RNLPLMALVLDMLVPPLALLTLLVTMLALIGALVAWFTGDAMPW
                     LLGLCIAALLAASVGLAWLGYGRQILSLRHLAYAPFYALAKLPLYARFLVNRQVEWVR
                     SRRDDGSASER"
BASE COUNT          544 a          693 c          727 g          323 t
ORIGIN      
        1 cccgctaccg agccaggtcg acatcaggaa cgggtggatc acatacaggg cgtatgagat
       61 gccggcgata tagaccagca gtcgttgctg cagcaacggt accagcggcg acgtcttatc
      121 cagcatggtc gcgccgatca aggccgcggc caggtaaggc ctcaggtaat tgaaggcgcc
      181 gctgtccgga tggcaggcga ccaaaagcag cggcaccagg acgagtaggc tgccctgcga
      241 gacgaagcgt cgcagcatcg cgtccggccg gctgttgtat atcagcgcca gccaggcgcc
      301 ggcgaggatc tcatcgacgc ggtaatggct gatcacggca tagtggatgc cgtcactgac
      361 gcgcagcagc gtgaaagcga cgcacagcag ggggatggcc agcaagccgc gcttgccgaa
      421 aaagccgacc agcagtgcga tgccaaggta gaaatgcacc tccacgcaca gcgaccacaa
      481 atgactggtg gtaaagatca gcggctctgg cggatagttg gcgtagaaca ggaaatgcgc
      541 gctccaggtc gcgccggagg ctggcgtcag gtagagcatc aatcccatgt agagccaggc
      601 caccgggagg atgcggaaga aacgccgcat gaggaaatcc ggcacactcg gccgctgcag
      661 caggaaatgg gtgatgagaa acccggaaag attgaagaac agcaccatgc cgagcagccc
      721 agcggccaca ttcagatccc aggccttcgg cccgagcggt aggaggtggg cggcaagcac
      781 cagcaggata ctgatgccgc gccagccatc cagcacccca aaatgttcct tatgcatgtg
      841 caagcccctt tccggatacg acgtcaggta ttcaatgccg cgcccagcgg tcgcttgcgc
      901 cgggcgaatc gcttgcgcgc gggttcttcg atgccgtaat aacaggcagc cgccaccatg
      961 gtcaccagca agaggaaggc gatcagggtc cacggcgcaa aactgtcggc aagaagatcg
     1021 aagcgccgct gcaggcgcac gaacaacgcc agcgtgaaga aatgcgagag atagagcgaa
     1081 tacgagatat cgccgaacca gctgaacggg cgcagggccg gcgtctctga ctggctaccg
     1141 cgatagaggg tcgccgcggc gatcagcatg aatgcggccg ttccccagta caaggtgcgg
     1201 tcataagcgg tgtgatggaa tatctgaccc aggacgaaca ggcctgatcc ggccagcagc
     1261 aaggccgccc cgccgccgat gcgcaagcct ctgctgtaca gcatggcgat cacgcaaccg
     1321 agcaggaact cgaacaccat gggctggctc accacttgcg cgatcagggc tgtgggttgc
     1381 agcagcatgc cgacccctac agtcaccgcg aaccatgccg tcatgaacat gaggcgaccg
     1441 gcaggccgca agccgatcgc cagcgtcaac gcgaacacca ggtagaaata ccactcgtag
     1501 gtcagtgtcc aggccatggg gatgacataa gccttgtgcg ccagcatgcc catgtcgatg
     1561 gccccgtcgt gataaggcag gaacagcatg gaggcgacga acttctgggg gctcaccacc
     1621 gtattcacgg cgctgcctgc ggccagcacc atggcgaagg cgatcgccgt cagcgtccag
     1681 tagagcggcg cgatgcgcac cagacggcgc ttgaggaaat cgcctgccga gggggtgcgc
     1741 gtcatctgcg aagtggtgat catcatgatg aagccactaa tgacgaagaa gatgtcgaca
     1801 ccggaggcgc cgaggttctc gagccagtgg ctcacctccg ccccacgctc gatctgcgag
     1861 gcgagataat gatgcagcac cacgagggag gcggccaggc cgcgcaatac ttgtacccag
     1921 acgaacatcg tcgttttctc cccttatctc tccgaagcgg aaccgtcatc acggcgtgag
     1981 cgcacccact cgacttgccg gttgaccagg aagcgcgcat acagcggtag cttggccaag
     2041 gcatagaacg gcgcgtaggc cagatgacgc aagctgagga tttgccggcc gtaaccgagc
     2101 caggcaagac cgaccgaggc tgcgagcaat gcggctatgc atagccccag cagccacggc
     2161 atggcatcgc cggtgaacca ggcgaccaac gcgccgatga gcgcgagcat ggtcaccagc
     2221 aaggtcagca gagccagcgg cggcaccagc atatccagca ccagcgccat caacggcagg
     2281 ttgcgct
//
LOCUS       DATMTW010000156         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_281451, whole genome shotgun sequence.
ACCESSION   DATMTW010000156 DATMTW010000000
VERSION     DATMTW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281451"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..2607)
                     /locus_tag="VN084_02885"
     CDS             complement(109..2607)
                     /locus_tag="VN084_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWT28432.1"
                     /translation="MEHSNQLPLQGVNVIDFGQYIAGPAVAMILADLGATVVHIDPQG
                     GPLWDSPANATLNRNKLCMRLDLKTPEGLHQARELISRADIVIENFRPGVMPRLGLDF
                     AELRRARPELITLSIPGFASDDELRAQWRATEAVIASASGVFTDMGQNRVLMGINPSF
                     SPLPLASAYGTMLAVSSVVLALQAREKNGVGDQIEVPLASAVMEGLSYNSIKIDGYPL
                     RYKTLREKESERRRAENLPMNLSYQDLQEFLDPFYRTYECADGRKFYAVCPSHREHAK
                     RCLQAMGIYEEMVAAGLPKVDDPYLPIDQWEGNASLGVYPLPADWAARISARMKEVFL
                     TRTAAEWEVIFGEGRFPGAPHRSTQEWLHDEHSNTAGLIVEVNDREFGVMKQPGPIAW
                     LEGCSEPMLKPLSRREVSYEQALATLSERSAPALPPAKLDKPAGWLEGVKILDLTNVI
                     AGPHSTSFLGRFGADVIKLDPVVPNYDPWNTVVFGMSSARGKESVLVDLNSCEGREVF
                     NRLVREVDVIVMNAPDRQLAPLGLDEASLQAVNPGVIFCQLDCFGGPRRGPRTDYLGY
                     DDLIQATTGIMLRFGGGMETPEEHAHVGTIDVMCGFAAALGVATALYRKHHSGEVYRA
                     RTSLASLGNLVQIPFCYDYAGRAPFNEPSGRDVVGYSDLSRFYKTADGWIYLDASEPE
                     LHKFADIKEFSGLASASDRAAYLQAVIARMSSSFLQERLQAANIAAAVPDNIDHLRSQ
                     YSRAADGLPGTENGSYSFSVYSDHPSGHRLTQLDPYAIRPRVARIRALPPAEKFGAST
                     RKVLGRYGYTEAQIQALLDAGAIGDSWSEEYLPS"
BASE COUNT          608 a          822 c          789 g          531 t
ORIGIN      
        1 tgggcatgaa cgcgcccgcg agcagccttt ggctcgtgtt cgcttgaacg gtaagtcggc
       61 aagcaagggg ggctctcgaa ggcgcgcccc ttgacctgcc ttcaggagtc agctgggcag
      121 atattcctca ctccaggaat caccgattgc gccggcgtcc aggagcgcct gaatctgcgc
      181 ctcggtatag ccgtagcggc ccaatacctt gcgagtcgaa gcgccaaact tctcagcggg
      241 tggcagcgct cgaattcgcg ctacccgcgg gcgaatggca taaggatcca gctgcgtaag
      301 ccgatgaccg ctcggatggt cgctgtagac gctgaaggag tagctgccat tctcggtacc
      361 tggcaggcca tcagcggcac ggctgtactg gctgcgcagg tgatcgatgt tgtccggcac
      421 cgcggcagcg atgttggccg cctgcagacg ctcctgaagg aaagagctgc tcatgcgggc
      481 aatgaccgct tgcagatagg cagcgcgatc actggcggac gccaagccgc tgaactcttt
      541 gatgtcggcg aatttgtgca gttctggctc ggacgcatcc aggtagatcc agccatccgc
      601 cgtcttgtag aagcgcgaaa gatcgctgta gccaaccacg tcgcgcccgg acggctcatt
      661 gaatggcgct cgacctgcgt agtcatagca gaacgggatc tgcaccagat tccccagtga
      721 agccagagag gtgcgcgccc ggtagacttc gccggagtgg tgtttgcgat aaagcgccgt
      781 tgcaacgccc agcgcggcag caaacccaca catcacatcg atggttccga catgggcatg
      841 ttcttccggg gtttccatgc caccaccaaa acgcagcatg atcccggtcg tggcctggat
      901 gagatcgtca taccccagat aatcggtgcg agggccacgg cgggggccac cgaaacaatc
      961 gagctggcag aagatcactc caggattcac cgcctgaagg ctggcctcat ccaggcccag
     1021 cggtgccagt tggcgatcag gcgcgttcat gacgatgaca tcgacctccc gcacaagacg
     1081 attgaacacc tctcgccctt cgcaagagtt caagtcaacc agtacacttt ccttgccccg
     1141 tgccgaagac ataccgaaga ccacggtatt ccagggatca tagttaggaa cgacagggtc
     1201 gagcttgatg acgtctgccc cgaagcggcc taggaacgac gtcgagtggg gccccgcgat
     1261 gacgttggtc aggtctagaa tcttgacgcc ttcaagccag ccggcaggtt tgtcgagctt
     1321 ggcaggcggc aaggcaggag ccgaacgctc ggacagtgtt gccaacgctt gctcatagct
     1381 cacctcgcga cgcgaaagag gcttcagcat cggctctgaa cacccctcca accaggcaat
     1441 tggaccaggc tgtttcatga cgccaaactc gcgatcattg acctcaacga tcagcccggc
     1501 agtattcgaa tgctcgtcat ggagccactc ctgggtcgac ctgtgcggag ccccagggaa
     1561 acgcccctca ccgaagatga cttcccactc cgccgctgtc ctggtcagga atacttcttt
     1621 catccgcgcg gaaatgcgtg cggcccaatc agcaggcagc gggtagacac ccaacgatgc
     1681 attaccttcc cactggtcaa tgggcaggta ggggtcatca accttgggca aaccggcggc
     1741 aaccatttcc tcgtagatgc ccatagcctg cagacagcgc ttggcatgct cacgatgcga
     1801 tgggcaaacg gcgtagaatt tacgaccatc cgcacattca taggtacggt agaacgggtc
     1861 gaggaactcc tgcagatcct gatagctcaa gttcatcggc aggttctcgg cgcgacgacg
     1921 ctcactctcc ttctctcgca gggtcttgta gcgcagcggg taaccatcga tcttgatcga
     1981 gttgtacgac agaccttcca tcaccgctga tgccaaggga acttcgatct gatcgccaac
     2041 gccattcttt tcacgcgcct gcagagcaag cactacagaa gatacagcaa gcatggtgcc
     2101 gtacgcagac gccaagggaa gcggcgagaa actagggttg atacccatca gtacgcggtt
     2161 ttgacccatg tcggtgaaca caccggaggc cgaagcgatc accgcctcgg tagcacgcca
     2221 ttgcgcacgc agttcgtcat cactggcaaa ccctggaatc gacaacgtga tcaactcagg
     2281 acgagcacgc cgcagctctg cgaaatcgag gcccagcctg ggcatgacac caggccggaa
     2341 attttcaatc acgatatcgg cacgggagat gagttcgcga gcctgatgca gcccctctgg
     2401 cgttttcaga tccagccgca tgcacagttt gttgcgatta agcgtggcat ttgccgggct
     2461 atcccagaga ggccctccct gcgggtcgat gtgaacgaca gtcgctccca gatcagcgag
     2521 gatcatcgct actgcggggc ctgcgatgta ttgtccgaaa tcgatgacat tgacgccttg
     2581 caaaggcaat tggttggagt gttccatgac gataacccta ggtagccccg tgccaacgag
     2641 attcgtcggc gcggctattg ttgttattga gacctcgtac taaccggtca cctggacgac
     2701 tgcaccatcc aagggcttgc gatgtgattg ctactttcag aaattcgaag
//
LOCUS       DATMTW010000157         5192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_584128, whole genome shotgun sequence.
ACCESSION   DATMTW010000157 DATMTW010000000
VERSION     DATMTW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584128"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..278
                     /locus_tag="VN084_02890"
     CDS             108..278
                     /locus_tag="VN084_02890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT28433.1"
                     /translation="MECIAREEGISINFTISIGVFSLKDKGTTLDVLLSQVDAAMYEA
                     KQSGRNKVTIAT"
     gene            complement(287..1003)
                     /locus_tag="VN084_02895"
     CDS             complement(287..1003)
                     /locus_tag="VN084_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWT28434.1"
                     /translation="MQLERILHRQGFGSRKLCRILVLNDQVTVNGAPCDDPSADFDTT
                     DLEFTVEGVAWQYREQAYLMLHKPAHYECSHKTLHHPSIYRLLPAPLVERGVQCIGRL
                     DEDTTGLILLSDDGQFIHRMSSPKWKVPKIYEVTTKHAVDDKQVQALLQGVLLVDESE
                     TIAALACSQVSDHVIHLTLAEGKYHQVKRMLAAVGNRVEALKRIRIGALELPADLAPG
                     EWRWLDKTDMENLRGEKLPA"
     gene            complement(1064..4042)
                     /locus_tag="VN084_02900"
     CDS             complement(1064..4042)
                     /locus_tag="VN084_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28435.1"
                     /translation="MKKPVSSGLGGNESPELTHLNHQLSSLVEARDAFYTSLLFRYGL
                     RETELTKYLVALAIFIIAFLARLLLIPDNSGLPYITFYPAIFVCAFVCGTRPSLLLVL
                     LSTFAGQFAFTPPLWSFKDTSEYASAMLLFALSGVLVCTVIEEIYRGVAAASDVEDKV
                     KETIRVLENDIRARAQTEEALRESEHLFRSLFEQAGVGVSLCDAVSGHFIKTNQRYCD
                     IVGYSLEELRALKLTNITHADDMAVQARLTKAMLAGEVKEYHIEKRYLKKDGSIVWVD
                     LTCTALWSPGESPTRHIAVVQDITEKKAIEAALQLSEQRWKFALDVTEEGVWDIDLNT
                     NRIFLSKRCKEMLGYAEDEIDDNVDAWASLIHPDDIDCLMAMRQTSIDGTHRTFTNEH
                     RKRCKDGSWRWVMVKGMVVDRDAAGNATRMIGTYTDIHASKEASDRLALADMVFQSSS
                     EAIMIADEHDRLFSINPAFTKVTGYDLDDVHGKSLHFLDRGLQSPAFYKEMQRQLDAT
                     GQWQGELRGQRKNGEVFAEWLTINSSLNADSSLHRRVAIFSDITKLKESEELVWQEAN
                     FDALTGLPNRRMFYDRLQLELKKSSRTGLPLAMMFMDLDRFKEINDSLGHDMGDVLLK
                     ETAKRLRRCVRETDIIARLGGDEFTVILSALDDPGVIERVASDILFQLSQPYQLGSEK
                     TYVTASIGITLYPQDAMGIDELMKNADRAMYAAKSLGRNRASYFTISMQEAAEKRLRL
                     TNDLRQAIPGKQLELHYQPIVDLRDGKVHKAEALLRWHHPHRGMVSPAEFIPIAEETG
                     LIVEIGDWVFENAVAEVARIRAQQPDFQISINKSAVQFRAEQDVHTHWFEHIARLGLP
                     GNALVVEITEGLLLDPSETTQTQLHQLNDAGIEIAIDDFGTGYSSLAYIKNFSIDYIK
                     IDQSFTRNLAPDSSDLALCEAIIVMAHKLGMRVIAEGVETAEQCALLTEAGCDYGQGY
                     LFSRPLPSEQFMSFLGQPAA"
     gene            complement(4128..5132)
                     /gene="dhaK"
                     /locus_tag="VN084_02905"
     CDS             complement(4128..5132)
                     /gene="dhaK"
                     /locus_tag="VN084_02905"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HWT28436.1"
                     /translation="MKKFINNVDQILVESLGGFAVAHHDLVDLHLDPNYLTRATPARD
                     KVALISGGGSGHEPLHAGYVGYGMLDAACPGHVFTSPSPDQMLAAAETVHAGKGVLFI
                     VKNYAGDVMNFEMAAEMLPFESATVLTSDDVAVTNSTYTTGRRGVAGTVIVEKCVGSL
                     AETGADLAACKALGDKVNRRTGSMGVALTSCTVPAAGRPTFDIGDTELEMGVGIHGEP
                     GRRREPMRAADAIVADLVEAILQDFEASGQKPGKGQEVLLLVNGFAGTPLMELYLLYN
                     TAARLLDQHGLTVSRSLVGNYVTSLEMAGASVTVCLLDDEIKKHWDSPVHTAALRWGR
                     "
BASE COUNT          971 a         1471 c         1647 g         1103 t
ORIGIN      
        1 agtagggaat agaccgctat gctgattggt gttacatagg gaagctgcat tatacgcagt
       61 ttcacccgca aggtaagtcg agagttggtt ttattgtatg taaatcattg gaatgcatcg
      121 cacgggaaga gggcatttca atcaatttca ccatctcgat cggggtattt agcctgaagg
      181 ataaaggtac cacccttgat gtcctgctga gccaggtaga tgccgcgatg tatgaggcca
      241 agcagtcagg gcgcaacaag gtcaccatag cgacctgagc gtcttgtcag gctggcagct
      301 tctcgcccct gaggttttcc atgtccgttt tatcgagcca acgccattcg cccggcgcga
      361 ggtccgccgg caactccagc gcgccgatcc ggatacgctt gagggcttcg acgcgattgc
      421 cgacagccgc cagcatacgc ttcacctgat ggtacttgcc ctcggccagc gtcaggtgga
      481 tgacgtgatc cgagacctgc gagcaggcca acgcggcgat ggtctccgat tcgtccacca
      541 gcaatacccc ctgcagcaaa gcctgcacct gtttgtcatc gaccgcatgc ttggtagtga
      601 cttcatagat tttgggcact ttccacttgg gggaactcat gcgatggatg aactggccgt
      661 catccgacaa caggatcagg cccgtggtgt cctcatccag ccggccgatg cactgcacgc
      721 cgcgctcgac cagcggcgcc ggcagcaagc gatagatgct gggatggtgc agggtcttgt
      781 gcgaacactc gtaatgcgcc ggcttgtgca gcatgaggta ggcctgctcg cgatattgcc
      841 aggcgacccc ttcgaccgtg aattccaggt cggtggtgtc gaaatcagcg cttggatcgt
      901 cgcagggcgc gccattgacg gttacctgat cgttgagaac aaggatacgg cagagcttgc
      961 gggagccgaa accctggcga tggaggatac gttcgagttg catgccgcca ttgtaaacga
     1021 gcccggccgg ctgatgtcgc atgccgggcg ctgcgagggg atgtcaggcc gcgggctggc
     1081 cgaggaagct catgaactgt tcgctcggca ggggccgcga gaacaggtaa ccctggccat
     1141 agtcgcagcc ggcctcagtc agcaaggcgc attgctcggc ggtttcgaca ccctcggcga
     1201 tgacgcgcat gccgagcttg tgcgccatga cgatgatcgc ctcgcatagt gcgaggtcgc
     1261 tggaatcagg cgcaaggttg cgggtgaagg attggtcgat cttgatgtag tcgatgctga
     1321 aattcttgat gtaggccagc gacgaatagc cggtgccgaa atcgtcgatc gcgatctcga
     1381 tgccagcatc gttgagttga tgcaactgtg tctgggtcgt ttcgctcggg tcgagcagca
     1441 ggccttcggt gatctcgacg accagcgcgt tgcccggcag gccgaggcgc gcgatatgct
     1501 cgaaccagtg ggtgtgcacg tcctgctcgg cgcggaactg cactgccgat ttattgatgc
     1561 tgatctggaa atccggctgc tgcgcacgga tacgcgctac ttccgcaacg gcgttctcga
     1621 acacccaatc gccgatctcg acgatgaggc cagtctcttc tgcgatgggg atgaattctg
     1681 ccgggctcac catgccgcga tgcgggtggt gccagcgcaa cagcgcttcg gccttgtgta
     1741 ccttgccgtc cctgaggtcg acgatgggct ggtaatgcag ttcgagctgt ttgcccggga
     1801 tcgcctgccg caagtcgttg gtcagccgca ggcgcttctc cgccgcctcc tgcatggaga
     1861 tggtgaaata gctggcacgg ttgcgcccca ggctcttggc tgcgtacatg gcgcggtctg
     1921 cattcttcat caattcatcg atacccatcg cgtcctgcgg gtagagcgtg atgccgatgc
     1981 tggccgtgac ataggtcttt tcgctgccga gctggtaagg ctgcgatagc tggaacagga
     2041 tatcggaagc gacgcgctcg atgacgccgg gatcatccag cgcgctcagg atgacggtga
     2101 actcatcgcc gcctaggcgc gcgatgatgt cggtctcgcg tacgcaacgg cgcaggcgtt
     2161 tcgccgtctc tttcagcagc acgtcaccca tgtcatggcc aaggctgtcg ttgatctcct
     2221 tgaagcggtc gaggtccatg aacatcatcg ccaggggcag gccggtacgg ctcgatttct
     2281 tcagctccag ctgcagccga tcgtagaaca tgcgccggtt gggcaggccg gtgagggcgt
     2341 cgaagttggc ttcctgccag accagctcct cggactcttt gagcttagtg atgtccgaga
     2401 agatggcgac gcggcgatgc agcgagctgt ccgcattgag gctggaattg atggtcagcc
     2461 actcggcgaa gacctcgccg ttcttgcgct ggccgcgcaa ctcgccctgc cattggccgg
     2521 tggcgtcgag ttgccgttgc atctccttgt agaaagcggg tgactgcagg ccgcgatcga
     2581 gaaaatgcag ggatttgccg tgtacgtcgt cgagatcgta gccggtgacc ttggtgaaag
     2641 cagggttgat cgagaacagg cgatcatgtt cgtcagcgat catgatcgct tcgctgctgc
     2701 tctggaagac catatccgcc agggccaggc ggtcgctggc ttctttcgag gcatggatgt
     2761 cggtataggt accgatcatg cgggtggcgt taccggcggc atcgcgatcg accaccattc
     2821 ctttcaccat cacccatcgc cagctgccgt ccttgcagcg tttgcggtgc tcgttggtga
     2881 aagtgcgatg ggtgccgtcg atggatgttt gccgcatcgc catcaggcag tcgatgtcgt
     2941 ccgggtggat aaggctggcc cacgcatcca cattgtcgtc gatctcatcc tcggcatagc
     3001 cgagcatctc cttgcagcgt ttgctgagga agatgcggtt cgtgttcagg tcgatatccc
     3061 agacgccttc ctcggtgacg tcgagcgcga atttccagcg ttgctcgctg agttgcagcg
     3121 cggcttcgat ggctttcttc tcggtgatgt cctgcaccac ggcgatatgc cgggtgggcg
     3181 attcgccggg cgaccacagg gcggtgcagg tcagatcgac ccagacgatg ctgccgtcct
     3241 ttttcaggta gcgcttctcg atatggtatt ccttgacctc acccgccagc atggccttgg
     3301 tcagcctggc ttgcaccgcc atgtcgtcgg catgggtgat gttggtgagc ttgagggccc
     3361 tcagttcttc caggctgtag ccgacgatgt cgcagtagcg ctggttggtc ttgatgaagt
     3421 ggccgctgac ggcatcgcat agcgagacgc cgacgccggc ctgctcgaac aggctacgga
     3481 acaagtgctc gctttcgcgc agcgcttcct cggtctgggc ccgtgcgcgg atatcgttct
     3541 ccagcacgcg gatggtttcc ttcaccttat cctcgacatc gctggcggcc gcgacccctc
     3601 gatagatctc ttcgatgact gtgcacacca gcacgccaga caaggcgaac agcagcatgg
     3661 cgctggcata ctccgacgtg tccttgaacg accatagtgg tggggtgaag gcgaactggc
     3721 cggcgaaggt gctgagcagg accagtaaca ggctggggcg tgtgccgcag acaaaggcac
     3781 agacgaagat ggcaggatag aacgtgatgt agggcaggcc gctgttatca gggatgagta
     3841 gcaggcgggc gaggaaggcg atgatgaaga tcgccagtgc gaccaggtat ttggtgagtt
     3901 cggtttcgcg taggccatag cgaaacagca gtgaagtgta aaaagcgtct cttgcctcaa
     3961 ccaggctgct gagctgatgg ttcaggtgtg taagttcagg gctttcgttg ccacctaggc
     4021 ccgatgaaac cggtttcttc atgcattccc ctagaccaat ccattctgtg cttcggcata
     4081 agggaatgca agcgggttta taaaataaaa accggggctt atggcgttta gcgaccccag
     4141 cgcaaggcag cggtatgtac cgggctgtcc cagtgctttt tgatctcgtc gtcgagcagg
     4201 cagaccgtga ccgaagcgcc tgccatctcc agcgaggtga cgtaattgcc gaccagcgag
     4261 cgactgaccg tcaggccatg ctggtccaag aggcgggccg cggtgttata gagcaggtag
     4321 agctccatca gcggcgtacc ggcgaagccg ttgaccagca gcaatacctc ctggcctttg
     4381 cccggctttt gtcccgaagc ctcgaaatcc tgcaggatcg cttctaccag atcggcgacg
     4441 atagcatcgg cggcacgcat gggttcgcgt ctacgcccgg gctcgccgtg gatgccgacc
     4501 cccatctcga gttcggtgtc gccgatgtcg aaggtcgggc ggccagcagc cggaacggtg
     4561 caactggtca gtgccacgcc catggagccg gtacgccggt tgaccttgtc gcccaaggcc
     4621 ttgcaggcgg cgaggtcggc gccagtctcg gccaggctgc cgacgcattt ctcgacgatg
     4681 accgtgccgg cgacgccgcg gcggccggtg gtgtaggtgg agttggtgac cgcgacatcg
     4741 tcgctggtga gtaccgtggc gctctcgaaa ggcagcatct ccgcggccat ctcgaagttc
     4801 atgacgtcgc cggcgtaatt cttgacgatg aacagcacgc ctttgccggc gtgcacggtt
     4861 tccgcagccg ccagcatctg gtcgggtgaa ggcgaggtga agacatggcc aggacaggct
     4921 gcgtcgagca tgccgtagcc gacataaccg gcatgcagtg gttcgtgtcc cgagccgcca
     4981 ccagagatca gcgctacctt gtcgcgggcg ggcgtcgcgc gcgtcaggta gttggggtcc
     5041 agatgcagat cgaccaggtc gtgatgggcg acagcaaaac cgccgaggct ctcgaccagg
     5101 atttgatcga cgttattgat gaatttcttc atggttcctc ctctactcca atgcggtacg
     5161 caaaatcaat cgattgcaca aggcatcgtt tg
//
LOCUS       DATMTW010000158         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_281454, whole genome shotgun sequence.
ACCESSION   DATMTW010000158 DATMTW010000000
VERSION     DATMTW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281454"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VN084_02910"
     CDS             <1..164
                     /locus_tag="VN084_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWT28437.1"
                     /translation="FNPFFYMIDGFRYGFFGTGDISPVISLSVVGGSFFALSWLTLKM
                     LKSGYKLRG"
     gene            175..423
                     /locus_tag="VN084_02915"
     CDS             175..423
                     /locus_tag="VN084_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HWT28438.1"
                     /translation="MLRAEELRDYIMQGLACDHVQVMGDDGQHFEAVIVSPQFAGKNM
                     VQQHQMVYQALGDRMREEIHALSMRTFTPEAWARFIEK"
     gene            534..851
                     /gene="grxD"
                     /locus_tag="VN084_02920"
     CDS             534..851
                     /gene="grxD"
                     /locus_tag="VN084_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HWT28439.1"
                     /translation="MDTQEIIKNQIKANPVVLYMKGSPKFPQCGFSGLAVQILQASGV
                     KDFVAVDVLADPAIRDGIKVYANWPTIPQLYIKGEFIGGADIMRDLYEQGELQKLLQE
                     AVA"
     gene            852..2120
                     /gene="murA"
                     /locus_tag="VN084_02925"
     CDS             852..2120
                     /gene="murA"
                     /locus_tag="VN084_02925"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWT28440.1"
                     /translation="MDKLVIEGGVPLRGEVTISGAKNAALPIICAALLAETPLHLTSV
                     PALRDVGTTYHLLQHMGVAVSKQGANAVTLDASNITAHEAPYEMVKTMRASILVLGPL
                     LARFGHARVSLPGGCAIGSRPVDLHIKGLKAMGAEIHIEHGYIEATTAHLPNKRLQGA
                     RIFMDIVTVTGTENLMMAAALAEGTTVLENAAREPEVVDMAECLIKMGARIEGVGTDV
                     ITVHGVERLQGCEHAIICDRIEAGTYMVAAAMTGGQVTLRNARADLLDAVIEKLREAG
                     VRVEHDAHSITVTGGGKLKSVNIRTAPHPAFPTDMQAQFMALNTIAEGAGQVIETIFE
                     NRFMHVQEMQRLGADIHVEGNTAIVRGVDHLDGAIVMATDLRASASLVLAALVARGET
                     TIERIYHLDRGYEQLEEKLTALGAKVRRVH"
     gene            2286..2930
                     /gene="hisG"
                     /locus_tag="VN084_02930"
     CDS             2286..2930
                     /gene="hisG"
                     /locus_tag="VN084_02930"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWT28441.1"
                     /translation="MITIALSKGRIFEETTPLLAAAGITALEDPESSRKLILPTNRDD
                     VRLIIVRATDVPTYVQYGAADLGIAGKDVLNEHGGEGLYQPLDLKIARCKMMVAVRED
                     FDYDGSIRRGARLKVVTKYVKTAREHFADKGMHVDLIKLYGSMELGPLVGLADAIVDL
                     VSTGGTLRANNLKAVEEITDISSRLIVNQASLKLKRGALQPIMDSFAKAVADNN"
     gene            2938..>4047
                     /gene="hisD"
                     /locus_tag="VN084_02935"
     CDS             2938..>4047
                     /gene="hisD"
                     /locus_tag="VN084_02935"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWT28442.1"
                     /translation="MDIRRLSTTDAGFEQALKDLLAFETAQDDKIDQVVADILRDVKT
                     RGDAAVLEYTHRFDRLDADSMAALELSQQELKAALDSLPADQRKALREAADRVRSYHE
                     KQLMSSWTYTEDDGTLLGQQVTSLDRVGLYVPGGKAAYPSSVLMNAIPAKVAGVKELI
                     MVVPTPGGEKNALVLAAAAVCGVDRVFCIGGAQAVGALAYGTQTVPQVDKIVGPGNAY
                     VAAAKRRVFGVVGIDMVAGPSEILVICDGKTNPDWIAMDLFSQAEHDELAQAILLSPD
                     AAFLDQVKASIDRQVEEMPRKDIIRTSLESRGALIQVRDLEEAAKISNYIAPEHLESS
                     VDESLAFSKKIKHAGAIFMGRDTCESLGDYCAGPNH"
BASE COUNT          863 a         1116 c         1244 g          824 t
ORIGIN      
        1 atttcaatcc gttcttctac atgatcgacg gattccgcta tggctttttc gggaccgggg
       61 atatatcgcc ggtgatcagt ctaagcgttg tcggcggcag cttttttgcc ctatcatggc
      121 tgactttgaa aatgctgaaa tccggctata aattacgggg ttaggtaaca ctagatgctg
      181 cgcgccgaag agctacggga ttacatcatg cagggcctgg cttgtgatca tgtgcaggtg
      241 atgggggatg acgggcagca ttttgaggct gtcatcgtta gcccgcaatt cgccggcaag
      301 aatatggtgc agcagcacca gatggtctac caggctttgg gtgatcgcat gcgtgaagag
      361 atccatgcgc tatccatgcg aaccttcacg ccggaggctt gggccagatt catagaaaaa
      421 taagccgcgg atcaagcgcg cagggtaaga atatgcggat atcccatccg ccaaccgccg
      481 gcctcaaggc tggcataccg actttcaggc attcgatatt ggagaatagt gcaatggata
      541 cgcaagagat catcaagaat cagatcaagg ccaacccggt cgtgctctac atgaagggta
      601 gcccgaagtt cccgcaatgt ggcttctcgg gattggctgt acagatcctg caggcaagcg
      661 gcgtcaagga tttcgtcgca gtcgacgtgc tcgcagaccc cgccatccgt gatgggatca
      721 aggtctacgc caactggcca acgattccgc agctctatat caagggcgaa ttcatcggtg
      781 gcgccgacat catgcgtgac ctttacgaac agggtgaatt gcagaagctg ctgcaggaag
      841 cagtcgccta attggataag ctcgtcatcg aaggcggagt gccgctgcgg ggtgaggtca
      901 ccatctctgg cgccaagaac gctgccttgc ccatcatctg cgcggcgttg ttggcggaga
      961 cgccgctaca cctgaccagc gtgccggcgc tgcgtgatgt cggtaccacc tatcatttgt
     1021 tgcagcacat gggtgtcgcg gtcagcaagc aaggtgcgaa cgccgtcacg ctggacgcca
     1081 gcaacatcac tgcacatgaa gcaccctacg agatggtgaa gacgatgcgt gcttccatcc
     1141 ttgtgctggg gccgttgctg gcgcgtttcg gccatgcgcg cgtatccctg cccggcggct
     1201 gtgcgatcgg ctcgcgcccc gtggatctcc acatcaaggg tttgaaagcc atgggcgcgg
     1261 agatccacat cgagcatggc tacatcgaag cgacgacggc gcatctgccc aacaaacgct
     1321 tgcagggggc gcgtatcttc atggatatcg tcaccgtcac cggtaccgag aacctgatga
     1381 tggccgctgc gctggccgag ggtacgaccg tgctcgagaa tgcggctcgc gaaccggaag
     1441 tggtcgatat ggcggaatgt ctgatcaaga tgggcgccag gatcgaaggc gtcggtaccg
     1501 atgtcatcac agtgcatggc gtggaacgcc tgcagggctg tgagcatgcc atcatctgcg
     1561 accggatcga agcgggtacc tacatggtgg ccgcagcgat gactggtggc caggtgacgt
     1621 tgcggaacgc ccgcgccgat ctgctggatg ccgttatcga gaagctgcgt gaggcgggtg
     1681 tgcgcgtcga gcacgatgct cacagtatca ccgttaccgg gggtggcaag ctgaagtcgg
     1741 tgaatatccg tactgcaccg caccccgctt tcccgacgga tatgcaggca cagttcatgg
     1801 ccttgaatac tatcgccgag ggcgcaggcc aggtgatcga gaccatcttc gagaaccggt
     1861 ttatgcatgt gcaggagatg caacgcctgg gcgcagacat ccatgtcgaa ggcaataccg
     1921 cgatcgtgcg cggcgtcgat cacctggatg gcgccatcgt catggcgacg gatctgcgtg
     1981 cttctgccag tttggtgctg gcggcactgg tggcccgtgg cgagacgacg atcgaacgta
     2041 tctaccatct cgatcgcggt tacgaacagc ttgaagagaa actgacggca ctgggtgcca
     2101 aagtcaggcg cgtgcattaa acgagcggat catggcagtg gggtgcacgc cgcatggcgg
     2161 cgcgtaagta agccctgatt tgacggcaaa aagcactaca ataggtgttt gcggatgccg
     2221 ctggatggcc ttgggaaacc agagcgctat tcggaacaat tgcagttaac gataacttta
     2281 aaactatgat caccatcgca ttatccaaag gccgtatctt tgaagagacg acgccgttac
     2341 tggctgcggc aggcatcacg gcgctcgagg atcctgaatc ttcgcgcaag ctgatcctgc
     2401 cgaccaaccg cgatgacgtg cggctcatca tcgtgcgcgc caccgacgtc ccgacctatg
     2461 tgcaatacgg cgccgctgat ctcggtatcg ccggcaagga cgtactcaac gagcatggtg
     2521 gcgaaggcct gtatcagccg ctagacctca agatcgcacg ttgcaagatg atggtcgccg
     2581 tacgcgagga tttcgactat gacggctcca ttcgccgcgg ggcacggctg aaggtcgtca
     2641 ctaaatacgt caagaccgca cgcgagcatt tcgccgataa gggcatgcac gtcgacttga
     2701 tcaagctgta cggctccatg gagctgggcc cgctggtcgg gctggcggat gccatcgtcg
     2761 atctggtcag taccgggggt accttgcgcg cgaataatct caaggctgtg gaagagatca
     2821 ccgacatcag ctcgcggctg atcgtcaatc aggcttcgct caaactcaag cgtggcgctt
     2881 tgcaacctat catggatagt tttgccaaag ccgtcgcaga caacaactag gttcattatg
     2941 gatattcgac gtttatcaac taccgatgct ggcttcgagc aggcgctcaa ggatctgctg
     3001 gcttttgaaa cggcccagga tgacaagatc gatcaggtcg tggccgatat tttgcgcgat
     3061 gtgaaaaccc gaggtgatgc tgcggtgctg gaatacaccc atcgtttcga tcgactcgat
     3121 gccgatagca tggctgcgct ggagctgtct cagcaggaac tgaaggcggc gctggatagc
     3181 ctccctgccg accagcgcaa ggccctgcgg gaggccgctg accgcgtgcg cagctatcac
     3241 gagaagcagc tcatgtcgtc ctggacctat accgaagacg acggcacctt gcttggccag
     3301 caagtcacct cgctcgaccg cgttggtctc tacgtgccgg gcggcaaggc cgcttatccg
     3361 tcttcagtgc tcatgaacgc catcccggcc aaggtcgcgg gtgtgaagga gctcatcatg
     3421 gtcgtgccga cgccaggcgg cgagaagaat gcgctggtgc tggccgccgc tgcggtctgt
     3481 ggtgtcgatc gtgtattctg catcggtggt gcccaggctg ttggcgcgct ggcatatggc
     3541 acgcagaccg taccgcaggt ggacaagatc gtcggaccgg gtaacgctta tgttgcggca
     3601 gccaagcgcc gtgtgtttgg tgtggttggt atcgacatgg tggccgggcc gtccgagatc
     3661 ctggtcatct gcgatggcaa gaccaacccc gactggatcg ccatggacct gttctcgcaa
     3721 gccgagcacg atgaactggc gcaagctatc ttgttgtccc cggacgcggc attcctcgat
     3781 caagtcaaag ccagcatcga ccgtcaggtg gaagagatgc cgcgcaagga catcatccgc
     3841 acttcactgg aaagtcgagg tgcgctgatc caggtacgcg atctggaaga ggcggcgaag
     3901 atctccaact acatcgcgcc ggaacacctc gagtcgtcgg tcgatgaatc gctggcgttc
     3961 agcaagaaga tcaagcatgc cggcgcaatc ttcatggggc gtgatacttg cgaatcgctg
     4021 ggcgattact gtgccggccc caatcac
//
LOCUS       DATMTW010000159         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_349505, whole genome shotgun sequence.
ACCESSION   DATMTW010000159 DATMTW010000000
VERSION     DATMTW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349505"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VN084_02940"
     CDS             <1..129
                     /locus_tag="VN084_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HWT28443.1"
                     /translation="ITLLIRVYGGYPDGVAFAVLLMNLCVPLIDMYTQPRVFGHEQ"
     gene            145..795
                     /gene="rsxG"
                     /locus_tag="VN084_02945"
     CDS             145..795
                     /gene="rsxG"
                     /locus_tag="VN084_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HWT28444.1"
                     /translation="MDKTIIRHAVVTALILTAFTVVGTALLSGTFEATKSPIADSERQ
                     ATLHLLSQVMPASLHDNDLLADSLDLPATPELGLKSGARIYRARLQGAPAGVVLEAIA
                     PDGYSGEIKLLIGIALDGTLTGVRVLAHKETPGLGDYIDLAHSDWIKNFDGLSLAKIP
                     EAGWKVKKDGGQFDYMTGATITPRAVIKAVHKALQYYAQSHEQLFATQGAATQTGE"
     gene            798..1475
                     /locus_tag="VN084_02950"
     CDS             798..1475
                     /locus_tag="VN084_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HWT28445.1"
                     /translation="MSQYGEIVENGLWKQNPGVVQLLGLCPTLAVTSTAINGLSLGIA
                     TSLVMALSNASVSPVRKLVPDEIRIPVFILIIAALVTIVDLSMNAYLHPLYNVLGIFI
                     PLIVTNCIVLARVEAFAAKNPTPASALDGFVMGLGLTFVLTLLGAMREFVGKGTVLSG
                     LDLVFGPQAKSLIWHVIPDYPGFLLAILPPGAFIGLGLLIAGRNWVEQRRTAAQALPP
                     LAASPSH"
     gene            1505..2152
                     /gene="nth"
                     /locus_tag="VN084_02955"
     CDS             1505..2152
                     /gene="nth"
                     /locus_tag="VN084_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWT28446.1"
                     /translation="MNAEKRREIFRRLAIAIPKPETELIHHSTFELLIAVILSAQATD
                     KGVNLATARLFPLANTPQAILALGLDGLESHIRTIGLYRSKAKNILATCEMLVRLHGG
                     QVLDTREALERLPGVGRKTANVILNTAFGQPTIAVDTHIFRLGNRIGLAPGKTPLAVE
                     QKLMKTVPKEYLQDAHHLLILHGRYTCIARKPKCDACVIVDLCEYKAKPTQAVSA"
     gene            2149..3222
                     /locus_tag="VN084_02960"
     CDS             2149..3222
                     /locus_tag="VN084_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HWT28447.1"
                     /translation="MRVATGLAMGSEADPALATQAVSAAMDKAGLTIAGSVLLFLTSD
                     FARDPVPALRAAAKAANCTRVMGCSATGIFTEQEWVMDAPAAVAMVFGESFSLQAPTH
                     VETDAVVLTLAAPNAINTTWLSDPGQRFGGVSGDATGQGPFSVWENGKGAVAGHCEAV
                     MQGVRSAVAAAHGLKLLTRPQYVDRSEGYDLVELGGQAALQALREACPFEEPLPLHRL
                     MAVFGDNPEQIRQTRYQQAALVSGNEADGSVTLARPVQAGQYLSWALRETGTAQADLQ
                     QTAAQLEKKLAMKPHFGLLFSCLGRGPYFYGGVDMDLALLAKRYPGMPLIGFYGNGEI
                     APVNGQSQLLQYSAVLGLFAADA"
     gene            3223..>3367
                     /locus_tag="VN084_02965"
     CDS             3223..>3367
                     /locus_tag="VN084_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HWT28448.1"
                     /translation="MALFNPSRDQVRQFFFDTWAKFRQQQPLSDLEKIGVQVMQMHPE
                     YHAI"
BASE COUNT          623 a         1068 c         1054 g          622 t
ORIGIN      
        1 atcaccctgt tgatccgcgt ttatggcggc tatccggatg gcgtggcgtt tgccgtgctg
       61 ctgatgaacc tttgcgtgcc gctgatcgac atgtacaccc agccccgggt cttcgggcac
      121 gaacagtgag tcgactggac cgccatggat aaaaccatca tcagacatgc tgtcgtcacc
      181 gccttgatct tgacggcatt cacggtcgtc gggaccgcgc tgctgtccgg tacgttcgaa
      241 gcgaccaaat cgccgatcgc agacagcgag cggcaggcaa cactgcatct gttgtcgcag
      301 gtgatgccgg ccagcctgca tgacaacgac ctgctggccg atagcctgga cttgccagca
      361 acgccggaac tgggcctgaa gtcgggtgcg cgtatctacc gggcccgcct gcagggcgcg
      421 ccggctggcg tcgtgctgga ggcgatcgcc cctgacggct acagcggcga gatcaagctg
      481 ctgatcggca tcgccctcga cggcacgctc accggtgtgc gcgtgctggc gcataaggag
      541 acgccagggc tgggcgatta catcgacctt gcccacagcg actggatcaa gaactttgat
      601 ggcctgtccc ttgccaaaat cccggaagca ggctggaaag tgaaaaagga cggcggccag
      661 ttcgattaca tgacaggcgc gacgatcacc ccgcgtgccg tcatcaaggc cgtgcacaag
      721 gccttgcagt attacgcgca atcgcacgaa caactgtttg ccacgcaagg cgcggcgaca
      781 cagactggag agtaggcatg agccaatatg gtgagatcgt cgagaacggt ctatggaaac
      841 agaacccggg cgtggtacag ctgctgggcc tgtgcccgac gctggccgtc accagcacgg
      901 cgatcaacgg gctctcgctc ggcatcgcca cctcgctggt catggcgctg agcaacgcct
      961 cggtatcgcc ggtacgtaaa ctggtgccgg atgagatccg cattccggtg ttcatcctca
     1021 ttatcgcggc gctggtgact atcgtcgact tgtcgatgaa cgcctacttg catccgctgt
     1081 ataacgtgct gggcatcttc attccgctca tcgtcaccaa ctgtatcgtg ctggcccgcg
     1141 tggaagcctt cgccgccaag aacccgacgc cggcttcggc gctggacggg tttgtcatgg
     1201 gcctgggcct gaccttcgtg ctgaccttgc tcggcgccat gcgcgaattc gtcggcaagg
     1261 gcacggtgct ctctggtctt gaccttgtgt ttggcccgca agccaagtcg ctgatctggc
     1321 atgtgatccc ggattacccg ggcttcctgc tggctatcct gccaccgggc gcgttcatcg
     1381 gcctcggcct gctgattgcc gggcgcaact gggtcgagca gcgccgcacg gcggcgcagg
     1441 ccctgccacc gctcgccgcc agccccagcc actgagtgtc ggccaggatg cctgcggaga
     1501 aacgatgaac gcggagaaac gtcgcgagat cttccgtcgc ctggccatcg ccatccccaa
     1561 gcccgagacc gagctgatcc accacagcac ctttgagttg ctgatcgcgg tcatcctctc
     1621 tgcgcaggcc accgataagg gcgtcaatct cgcgaccgcc cggctattcc ccctcgccaa
     1681 tacgccgcag gccatcctcg cgcttgggct cgacggcctg gaaagccaca tccggaccat
     1741 cggtctctac cgtagcaagg caaagaacat cctcgccacc tgcgagatgc tggtccgtct
     1801 gcacggtggg caggtgctgg atacgcgcga ggcgctcgag cgccttcccg gcgtcgggcg
     1861 caagaccgcc aacgttatcc tgaacacggc cttcggccag ccgaccatcg ccgtcgatac
     1921 gcacatcttc cgactcggca atcgcatcgg cctcgcgccg ggcaagacgc cgctggcggt
     1981 cgagcagaaa ctcatgaaga ccgtccccaa ggaatatctg caggatgcgc accatttgct
     2041 gatcctgcat ggacgctaca cctgcatcgc tcgcaaaccg aaatgcgatg cgtgcgtcat
     2101 cgtcgacctc tgcgaataca aagccaaacc gacgcaggcg gttagcgcat gagagtcgcc
     2161 accgggctgg ccatgggtag cgaagccgat cctgcgttgg cgacccaggc cgtgagtgcg
     2221 gcgatggaca aagccggctt gacgatcgcc ggcagcgtgt tgctgttcct cacctcggat
     2281 ttcgcccgcg atccggtgcc cgcgctgcgt gcggcggcca aggcggcgaa ctgcacacgc
     2341 gtcatgggct gttctgccac gggcatcttc actgaacagg aatgggtgat ggacgcgccg
     2401 gctgctgtcg ccatggtgtt cggcgagagt ttcagcctgc aggcgccaac gcacgtggaa
     2461 accgatgccg tggtcctcac gctggcagca cccaacgcca tcaacaccac ctggttatcc
     2521 gaccccggcc agcgcttcgg cggcgtatcc ggcgatgcca ccggccaagg accgttctcg
     2581 gtgtgggaga acggcaaggg cgctgtcgcc ggacattgcg aagcggtgat gcagggtgtg
     2641 cgatccgctg tggccgcggc gcatggattg aagttgctga cgcgtccgca atacgtggat
     2701 cgcagcgaag gttacgatct tgtcgagctg ggcggacagg cggccttgca ggccctgcgg
     2761 gaagcctgcc catttgagga accgctgccg ttacatcgtt tgatggcggt gttcggcgac
     2821 aatccggagc agatccggca gacgcgctac cagcaggcgg cgctggtcag cggcaatgaa
     2881 gctgacggct cggtgactct ggccaggccg gtgcaggccg ggcaatacct gagctgggcc
     2941 ctgcgcgaga ccggcacggc acaggccgat ctgcagcaga cggcggcgca gcttgaaaag
     3001 aaactggcga tgaagccgca tttcggcttg ctgttctcct gcctgggacg tggtccgtat
     3061 ttctacggag gcgtcgacat ggatctggcc ctgttggcga agcgttatcc ggggatgccg
     3121 ctgatcggtt tctacggcaa cggcgagatc gccccggtca acggccaaag tcagctattg
     3181 cagtattcgg ccgtgctcgg gctgttcgcg gcggacgcct agatggcctt gttcaacccc
     3241 agccgcgatc aggtccggca attcttcttc gatacgtggg ccaaattccg ccagcaacag
     3301 ccattgagcg atctagagaa gatcggcgta caggtgatgc agatgcatcc cgaataccat
     3361 gcgattc
//
LOCUS       DATMTW010000160         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_13751, whole genome shotgun sequence.
ACCESSION   DATMTW010000160 DATMTW010000000
VERSION     DATMTW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13751"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VN084_02970"
     CDS             <1..220
                     /locus_tag="VN084_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWT28449.1"
                     /translation="GQLDSNFADMANIGGRAAGSITAACFLSRFTKKYDWAHLDIAGT
                     AWKSGKQKGATGRPVPLLTEFLVQRAGA"
     gene            236..682
                     /locus_tag="VN084_02975"
     CDS             236..682
                     /locus_tag="VN084_02975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HWT28450.1"
                     /translation="MTRVEFFFNVEDKLQKVAELSARAVGKGRRLHVYTPDAQVSEQL
                     GRQLWTQPALSFLPHCTAEDALADTTPVIIDSIGATLPHDDILINLQAGHPPFFSRFR
                     RLIEIVGQTESDKAEARKRYRFYRDRGYEIRAFDANGANLTNGGGQ"
     gene            679..1101
                     /locus_tag="VN084_02980"
     CDS             679..1101
                     /locus_tag="VN084_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28451.1"
                     /translation="MSDDPQNQQDLARVLSRIDALTKHPPSDKPLPQFDEGIPLLTEV
                     YEGTIPALQARPDLLPQAARIFPALNQIRLSEAEAQSLSPELVERLMTEMAPLIEEAV
                     KKAVKGELKAAQQALNTRLEAELVQTLRLRLQSALHEV"
     gene            complement(1164..1547)
                     /gene="gcvH"
                     /locus_tag="VN084_02985"
     CDS             complement(1164..1547)
                     /gene="gcvH"
                     /locus_tag="VN084_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWT28452.1"
                     /translation="MAIPTELHYTSEHLWTAPAEDGTWLAGITDYAQDLLGDIVFVEA
                     PAIGTRLEAGKPCGLVESVKTGSDLHAPRAGEVVAINEQLQGNPEQLNDKPYASWIFR
                     YRPSDPADANGLLDARAYEALLGQA"
     gene            1713..>2692
                     /gene="pbpG"
                     /locus_tag="VN084_02990"
     CDS             1713..>2692
                     /gene="pbpG"
                     /locus_tag="VN084_02990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HWT28453.1"
                     /translation="MLRIFMTMCIALSLIVPVTVVEAAQKTHKRVSTASKHKAATSRK
                     IIRTIRSDHKYRVQNVNYNRQSQVFNDDLDSNSALQLASSKALIVNQETGETVYAKNT
                     EVATPIASVTKLMTAMVMLDARLPMDETLTIAEGDVDTLKGTSSRLRLGTQLTRAELL
                     HLALMASENRAASALGRNYPGGLPAFVAAMNIKAIQLGMNNTHFVDPTGLNSENVSTA
                     EDLVKMVRAAYQYPEIRQVTTSTNMQVPVHGLRNPANFVNTNILVRNSDWVIGLSKTG
                     YINEAGRCLVMQAEIAGQPMIIVLLDSWGKYSRIGDAQRIRKWIESNNIMT"
BASE COUNT          597 a          802 c          759 g          534 t
ORIGIN      
        1 gggtcagctc gacagtaact ttgccgatat ggccaacatc ggcggtcgtg ccgccggcag
       61 catcaccgcc gcctgtttcc tttcacggtt caccaagaaa tatgactggg cccacctgga
      121 tatcgcaggt acggcctgga aatcgggtaa gcaaaagggg gcgacaggac gaccagtgcc
      181 cttactgacc gaattcctgg tgcagcgtgc tggcgcatag ccggcatcct cgggcatgac
      241 acgcgtcgaa ttctttttca acgtcgaaga caagctgcag aaagtcgcgg agctgagtgc
      301 gcgagcagta ggcaagggtc gccgcctgca tgtgtatacg ccggatgccc aagtctccga
      361 acagcttggc cgccagctat ggactcagcc ggcgctttct tttttgccgc attgcacggc
      421 ggaggacgcg ctggctgaca ccactccggt catcatcgac agcatcggcg cgactttgcc
      481 gcatgatgac atcctgatca acctgcaggc cggtcatcca ccgttcttca gtcgttttcg
      541 ccgcctgatc gagatcgtcg gccagactga atcagacaag gcagaagcac gtaaacgcta
      601 tcggttctac cgcgatcgcg gctacgagat ccgcgctttc gacgccaatg gcgccaatct
      661 gacgaatgga ggcggacaat gagcgacgac ccgcaaaacc agcaggatct tgccagggta
      721 ttgagccgga tcgacgcact gaccaagcat ccgccaagtg acaagccgtt gccgcagttc
      781 gacgaaggta tcccgctatt gacggaggtt tacgaaggca caataccggc gctgcaagcg
      841 cgccccgacc tgttgccgca ggcggcacgc atcttcccgg cgctgaacca gatacgcctc
      901 agcgaagcgg aggcccaatc cttgtcgcct gaactcgtcg agcgactgat gacggagatg
      961 gcgccactta tcgaggaggc agtgaaaaaa gcggtgaaag gtgagctgaa agccgcacag
     1021 caagccttga acacccggct agaggcggaa ctggtgcaga ccttgcgcct acgcttgcaa
     1081 tcggccctgc acgaagtctg atgaacacta tccggcgttg cgccagcgtt ccctttgttt
     1141 gcctgtgggt gggtgtgccc tgttcaggcc tggcctagca gcgcctcata ggcccgggca
     1201 tccagcagcc cgttggcatc agccggatcg ctcgggcggt acctgaagat ccagctcgca
     1261 tagggcttgt cattaagctg ctcgggatta ccctgtaatt gctcgttgat ggcgaccact
     1321 tcgccggcgc gcggtgcatg caagtctgag ccggtcttga ccgattcgac caggccgcaa
     1381 ggttttcccg cttccagtcg cgtgccgatg gcgggcgctt cgacaaagac gatgtcgccc
     1441 aatagatcct gcgcataatc ggtgatgccc gcgagccagg tgccgtcctc ggcaggtgcg
     1501 gtccaaagat gttcgctggt gtagtgtagt tcggtcggta tagccatagt gggtagatgc
     1561 ctgttcgatt cagatgctat tctcgcataa atatggactg tgtacttttg gcgacaatct
     1621 gagaaacttt gtaaagtaat gcgcgtatgt tgatgacaaa tcaaattatt ttggctaata
     1681 tcccctcgaa tgcttctcgg gggtgggcca caatgctacg aatattcatg acaatgtgca
     1741 tcgcattgag tttgatcgtc cctgtcacgg tggtcgaggc cgcgcaaaag acccacaagc
     1801 gtgtctccac cgccagcaag cacaaggctg cgaccagccg caagatcatc cgcaccatcc
     1861 gctctgacca caagtatcgc gtgcagaacg tcaactacaa tcgccagagc caggttttca
     1921 acgacgacct cgatagtaac agcgcactcc agctggcttc atccaaggca ctgatcgtta
     1981 accaggagac cggcgagacg gtctatgcca agaacaccga agtcgccaca ccgatcgcat
     2041 cggtgaccaa gctcatgacg gcgatggtga tgcttgatgc ccgcctgccg atggatgaga
     2101 ccctgaccat cgctgaaggc gatgtcgata ccctgaaggg caccagttcg cgtttacgcc
     2161 tcgggacgca gctgacccgt gcggaactac tgcacctcgc gctgatggct tcagagaacc
     2221 gtgccgcttc cgcgctggga cggaactacc ccggagggct gcctgccttt gttgccgcca
     2281 tgaacatcaa ggcgatccag ctcggcatga acaacaccca tttcgtcgac ccgaccggcc
     2341 ttaacagcga gaacgtctcg actgctgaag acctcgtcaa gatggtgcgt gcggcttacc
     2401 aatacccgga gatccgtcag gtcaccacat cgaccaacat gcaggtgccg gtgcacggtt
     2461 tgcgtaatcc cgcaaatttc gtcaacacca acatcctcgt acgcaacagc gattgggtga
     2521 tcggcctgtc caagaccggc tacatcaatg aagccggccg ctgtctggtc atgcaggcag
     2581 agatcgcggg ccaacccatg atcatcgtac tgctcgattc ctggggcaag tattcccgta
     2641 tcggcgatgc acagcgtatc cgcaaatgga tcgagagcaa caacatcatg ac
//
LOCUS       DATMTW010000161         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_517458, whole genome shotgun sequence.
ACCESSION   DATMTW010000161 DATMTW010000000
VERSION     DATMTW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517458"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="VN084_02995"
     CDS             complement(<1..1675)
                     /locus_tag="VN084_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28454.1"
                     /translation="MLFLVAASLFSQTSFAQLRLADQPVFTSVSIPGNLALTLSVEFP
                     TAVSVAHPTRTYSSTNNYLGYFDPAKCYKYRYVSDSADDNYFYPTRLATNRKCSGEWS
                     GNFLNWASMQTIDPFRWALTGGYRWIDTPELTVLEKAWSGGQGSASSNFPNSTTTGSE
                     IAGATPFTGAASFGTSILGWGNKMRFMAVASGSPSYSGTATHFSGTASLAKLYEVYVR
                     VKVCDSSVGLEADKCVAYSNGNYKPEGLMQKYANKIRYSTFGYLASDDGGDTNLKRDG
                     GVLRAAQRFIGPTQPVPGSSDIPNPLTPEWDSSTGVMTRNPNAADATASSTTTVPIVD
                     SGVMNYLNKFGEINRTVYKRYDPVSELYYAALRYFRNLGNVPAWSSMDGVTDAVRKTM
                     MDGFPVITQWKDPIQYSCQRNFILGIGDVNTHADRNLPGATTGSKEPAMPPEVFADTV
                     NAVDWTNKVGTLQGLTNLGTTLIPSNDAGYLMAGLAYYANTTDIRPDLEGKQTIKTYW
                     LDVLEGGYKTNRQFYLAAKYGGFNPPTSFSVDTATATQFADNKGWWSTTG"
     gene            complement(1745..2347)
                     /locus_tag="VN084_03000"
     CDS             complement(1745..2347)
                     /locus_tag="VN084_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28455.1"
                     /translation="MKTATRPNSMRQQRGVVLVFALIALVIMLIGAVAITRSINSSQF
                     NIGNIGFKRDLANQSERAVQLAMNAVKAGGALADPTKRLTALASANYSAVKLADNIQG
                     IPNILLQSDAQFATVGTAPDIDVDGLNVKVRYVVDRLATAEGACNSTNCTMTTAQVFG
                     GAASELNPVTPTPQPVYRLTVKLTGPRNTLSFFQSTFTTP"
     gene            complement(2349..3521)
                     /locus_tag="VN084_03005"
     CDS             complement(2349..3521)
                     /locus_tag="VN084_03005"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HWT28456.1"
                     /translation="MKEASILTRHCRAAWHAGFTLIELLVALVIGLALTLVITRMLAS
                     QESLRRGVTSGVDLASNSAYAAYTLERELRVAGAGFSQSVPANYGCLLNVSKNNAQML
                     PATAAFPAPFAAISTNYRLAPLVVYAGAGQGGSDVIAMATGGSALSGMGLPVVPKSTA
                     AGQLKLANTLGIQAGDLVLVSQQGAGCMVEQVSNGFVASTAAAPQPVLTFGGAYAADT
                     INGLSLTDLSTANAFVSVLGNVAGNRPRIQLLGLGSNATLFSYDLLQLTSNPLQALMD
                     GVVDLRVRYGLAPTKGSKVTTWTTPSAAGYTAAALTDGSGTAQANLQSIVAVRVGLIL
                     RSDLIEKDDVSATTLTLFGDLGDSLKYTYALPADGTRQRYRAVEFTVPLRNVRFSN"
     gene            complement(3518..>3763)
                     /locus_tag="VN084_03010"
     CDS             complement(3518..>3763)
                     /locus_tag="VN084_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28457.1"
                     /translation="LLASEIAATMVNQGTVNLEAAVVQNWADRVADPASGGLPGGVGT
                     VTVTSNIARVNIQWTPPQSKAGDANGVHNYQTDVVVP"
BASE COUNT          696 a         1088 c         1206 g          773 t
ORIGIN      
        1 ccccggtggt gctccaccag cccttgttgt cggcgaattg agtcgccgtc gcggtgtcga
       61 cggaaaacga ggtgggcgga ttgaagccgc cgtacttcgc ggcgaggtag aactggcgat
      121 tggtcttgta gcccccctcc aggacgtcca accaatacgt cttgatggtc tgctttcctt
      181 ccaggtcggg ccggatgtca gtggtgttgg cgtagtaggc caggcccgcc atcaggtatc
      241 ccgcatcatt gctggggatg agcgtggttc ccaggttcgt cagcccctgc agcgtgccga
      301 ccttgttggt ccagtccacg gcgtttacgg tatccgcgaa tacctctggt ggcatcgccg
      361 gctccttgct gccggtagtc gcaccgggaa ggttacggtc ggcatgggta ttgacgtcgc
      421 cgatgccgag aatgaagttt cgctggcaag aatactgaat gggatccttc cactgcgtaa
      481 tcaccgggaa cccatccatc atcgtttttc tcactgcatc cgtcaccccg tccatggatg
      541 accaggcggg cacgttgccg agatttctga agtagcgcaa ggcggcgtaa tacaactcgc
      601 tcaccgggtc atagcgtttg tagaccgtac ggttgatttc accgaatttg ttcagataat
      661 tcatcacgcc gctgtcgaca atcggcactg ttgtcgtact gctggcggtg gcatcggcgg
      721 cgttggggtt ccttgtcatc actccggtcg aagaatccca ttccggggtg agcggattgg
      781 ggatatcgga gctgccggga actggttgcg tcgggccgat gaaacgttgg gcagcgcgca
      841 agacgccgcc atcacgtttc aggttggtat cgccgccgtc atcacttgca agatagccga
      901 aggtgctgta acggatcttg ttggcatatt tctgcatcag tccttccggc ttgtaattgc
      961 cgttgctgta ggccacgcat ttgtctgcct cgagaccgac ggagctgtcg cagaccttga
     1021 cgcgtacata gacctcgtac agcttggcca gggacgcggt accgctgaaa tgggtggccg
     1081 ttccgctgta gctcggcgag cccgatgcaa cggccatgaa tcgcatcttg ttgccccagc
     1141 cgaggatcga ggtgccgaac gaggcggcac ctgtgaacgg cgtcgcacct gcgatttccg
     1201 accccgtggt cgtgctgttg gggaagttgc tggacgcaga accttgcccg ccgctccatg
     1261 ccttctcgag tacggtgagc tccggcgtgt cgatccagcg gtaccccccc gtcaaagccc
     1321 agcggaacgg gtcgatggtt tgcatcgatg cccagttcaa gaaattgccg ctccattcac
     1381 ccgagcattt gcgatttgtt gcaaggcgcg tgggatagaa gtagttgtcg tctgcggagt
     1441 cgctgacgta cctatattta tagcactttg ccggatcgaa ataaccgagg tagttgttcg
     1501 tcgatgagta ggtgcgggtg gggtgggcca cacttaccgc ggtcgggaat tcaacggaca
     1561 gcgttagcgc cagattgccg gggatactga ccgacgtgaa cactggctga tccgcaagcc
     1621 tcaactgggc gaacgaagtc tggctgaaaa ggctcgccgc gacgaggaac agcaatctct
     1681 catagcgcct gccacgcgcc aaaaagatat tattcatggc gtgatctccc ggttctaggc
     1741 gacctcaggg ggtcgtgaac gtcgattgaa agaaggaaag ggtgttgcgc gggcctgtca
     1801 gtttcacggt gagacgatag acaggctgcg gggtgggcgt caccgggttg agttcgctgg
     1861 ccgcgccgcc aaatacctgc gccgtcgtca tggtgcaatt ggtcgagttg catgcccctt
     1921 ctgcggtggc aaggcggtcg acgacgtacc gtactttgac attcagccca tcgacgtcga
     1981 tatccggcgc tgtaccgacc gtagcgaatt gcgcatcaga ctgcagcaat atgttgggaa
     2041 taccttgaat gttgtctgcg agcttgactg ccgaatagtt tgctgatgcc aatgccgtca
     2101 accgcttcgt cgggtcggcg agcgcaccgc ccgctttgac cgcattcata gccagctgga
     2161 cggcgcgctc ggattgattg gcgaggtctc gcttgaagcc aatattgccg atgttgaact
     2221 gactcgaatt gatggagcga gtaatggcca cggcgccgat gagcatgatc accagcgcga
     2281 tcaaggcaaa caccagcacg acgccgcgct gctgccgcat ggagttgggc cgagtggcag
     2341 tcttcatgtc agttggagaa gcgaacgttg cggagaggca ccgtgaattc cacggcccga
     2401 taccgctgac gggtgccgtc ggcgggaagc gcgtaggtgt acttcagcga gtcgccgagg
     2461 tcgccgaaca gcgtcagagt ggtggcagag acgtcgtcct tctcaatgag gtcgctgcgc
     2521 aagatcaggc cgacgcggac agccacaatg ctctgcagat tcgcctgggc ggtgccgctg
     2581 ccgtcagtca gcgctgccgc cgtgtagccg gcagccgagg gggtggtcca cgtcgtgacc
     2641 ttgctaccct tggtcggagc caggccgtag cgaacacgca aatccacgac gccgtccatc
     2701 aaggcttgga gcggattcga tgtaagttgc agcaggtcgt agctgaacag ggtggcattt
     2761 gagcccagcc ctaggagttg gattcgcggc cgatttccgg ccacgttgcc cagtaccgat
     2821 acgaaggcat tggcggtgga caggtccgtc aaggagagtc cgttgatggt gtcggcggca
     2881 taggcgccgc caaaggtcag caccggctgg ggcgccgcag cagtgctcgc gacgaagccg
     2941 ttgctcacct gttcgaccat gcagcccgcg ccctgttgcg atacgagcac gaggtcgcct
     3001 gcctgtatgc cgagcgtgtt ggccagcttg agctggcctg cggccgtgga tttcggaacc
     3061 acgggcaaac ccatcccgct caaagcggag cccccggtcg ccatggcgat cacgtcggag
     3121 cctccttgac cggcgcccgc gtacaccacc agcggcgcaa gtcgatagtt ggtcgaaatc
     3181 gctgcgaacg gcgctggaaa tgctgcggtt gcgggcagca tctgggcgtt gttcttggag
     3241 acgttcagaa ggcagccata gtttgctggc acggattgac taaacccagc tccagcgaca
     3301 cggagttccc gctccagcgt gtaggctgca taggcgctgt tgctcgccag gtcgacgccc
     3361 gaggtgacgc cccgccgcag cgactcttgg ctggcgagca tgcgcgtgat gaccagggtc
     3421 agcgccaagc cgatgacgag ggccaccagg agctcgatca gtgtgaagcc ggcatgccag
     3481 gctgcccgac aatgccgcgt gagaatggag gcctccttca tggcaccacc acatcggtct
     3541 ggtagttgtg gacgccgttg gcgtcccccg ccttggactg gggaggcgtc cattggatgt
     3601 tcactcgcgc aatgttgctg gtgaccgtga cggtcccgac accgccgggc aggccccccg
     3661 aggctggatc ggcgacgcgg tccgcccagt tctgcacgac ggcggcctcc aggttgaccg
     3721 tgccctggtt gaccatcgtc gcggcgatct cgctggccaa cag
//
LOCUS       DATMTW010000162         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_315759, whole genome shotgun sequence.
ACCESSION   DATMTW010000162 DATMTW010000000
VERSION     DATMTW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315759"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VN084_03015"
     CDS             complement(<1..299)
                     /locus_tag="VN084_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWT28458.1"
                     /translation="MSKSADFSQIPKPTQANLQKLGIFDIQGLLLHLPLRYVDETRIT
                     AIRDLRLGDQVQVEGEIVHTEVQYKPRKTLISRLRDASGQQLTLRFLHFYPSQLAT"
     gene            complement(321..701)
                     /locus_tag="VN084_03020"
     CDS             complement(321..701)
                     /locus_tag="VN084_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWT28459.1"
                     /translation="MANKQIIQTAGAPQAIGTYSQAVKVGSTVYLSGQIGLDPASMQL
                     VDGIENQIHRVFHNLKAVAEASGGSLADIVKLNVFLTDLSHFALVNTIMAEYFTQPYP
                     ARAAVGVASLPKGALVEADGVIEL"
     gene            complement(726..2948)
                     /locus_tag="VN084_03025"
     CDS             complement(726..2948)
                     /locus_tag="VN084_03025"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWT28460.1"
                     /translation="MSRTTAKRPADAVKSEAPPLLPADSEDSRLTLRLKEYLKPEDIA
                     QVWAAYRFSAAAHEGQMRKSGEPYITHPVSVACILAELHMDTPTLVAALLHDVVEDTG
                     VTNQEVADQFGKQVAELVDGLSKLDKIEFQSATQAQAENFRKMLLAMSQDVRVILVKL
                     ADRLHNMQTMDAMSAEKRRRISRETLDIYAPIANRLGLNAIFQELEDLSFKYLYPMRY
                     RVIAKAIKAARGNRKEVVGKILEAIKQRLQEMHIDADVTGREKHLYSIYKKMTGKTTT
                     FSQIYDIYGFRVIVKDMPTCYLALGALHGLYKPIPGKFKDYIAIPKANGYQSLHTTLF
                     GPFGTPIEVQLRSQEMHNIAEAGVAAHWLYKTTDAHLTALQQQTHLWLQRLLEIQSES
                     ADSLEFLEHFKVDLFPDEVYVFTPKGNIMALPKGATAVDFAYAVHTDIGNRCVAVKIN
                     QELAPLRTELHNGDHVEIITAAHAHPNPAWLNYVVSGKARAHIRHFLKSMQTTESAYL
                     GERMLNQALRALHADISLITDESWQKLLRDYGAKSKEEILTDIGLGKRLNVMVAHQLM
                     AMAGEQGERHAKHTSKPLGTITIRGTEGMAVQFAQCCRPIPGDPILGFINKDKGLVIH
                     THDCPAIRKFRVEPERWLDVEWDPDGKRLFKVNLKLAVANQRGMLAKIAAGIADAGSN
                     IDNVSMEEPDDSAYSNVYFTVQVLDRVHLADLMRRLRKIPDVVRINRVKGAGNEQRVQ
                     "
     gene            complement(3025..3237)
                     /gene="rpoZ"
                     /locus_tag="VN084_03030"
     CDS             complement(3025..3237)
                     /gene="rpoZ"
                     /locus_tag="VN084_03030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWT28461.1"
                     /translation="MARITVDDCLDKIPNRFQLTLVAAYRARQLANGSEPMVNTHGQK
                     DKSTVLALREIAAGKVGLEVLNRGHA"
BASE COUNT          607 a          944 c         1107 g          730 t
ORIGIN      
        1 gtggccagct gactcggata aaaatgcaga aagcgcaagg tgagttgctg cccgctggcg
       61 tccctgagcc ggctgatgag tgtcttgcgc ggcttgtact ggacctcggt atggacgatc
      121 tcaccttcga cctgcacctg gtcgcccagc cgcagatcgc ggatggccgt gatgcgcgtc
      181 tcgtcgacat agcgtagcgg caggtgcagc agcaagccct ggatatcgaa gataccgagc
      241 ttctgcaggt tggcctgcgt cggcttgggg atctgcgaga aatcggcaga cttgctcagg
      301 gcgatcgctc ggctggccgt ttagagttcg atgacgccgt cagcttcgac caaggcccct
      361 ttgggcagtg aggcgacacc gaccgcagca cgggccggat aaggctgggt gaaatactcg
      421 gccatgatgg tgttgacgag ggcgaaatgg gacaggtcgg tgaggaagac attgagcttg
      481 acgatatcgg ccagcgatcc gcccgaagcc tctgccacgg ccttcaggtt atggaacacc
      541 cgatgtatct ggttttcgat gccatcgacg agctgcatgc tggccggatc gagcccgatc
      601 tggccggaga ggtagaccgt gctgccgacc ttgacggctt gggaataggt gccgatcgcc
      661 tgcggggcgc ctgcggtctg gatgatttgc ttgtttgcca tgtggactct ctgttgaatg
      721 aaaacttact ggacgcgttg ctcgttgccg gcgcccttga cgcgattgat acgcaccacg
      781 tcggggatct tgcgcaggcg ccgcatgaga tcggccagat gcacacggtc cagcacctgc
      841 acggtgaaat agacattact gtaggcgctg tcgtccggct cttccatgct gacgttgtcg
      901 atgttggacc ccgcatcggc gatgccggca gcgatcttgg ccagcatgcc gcgctggttg
      961 gccacggcca gtttgaggtt gaccttgaac aggcgcttgc cgtccggatc ccattccacg
     1021 tcgagccatc gttccggttc gacgcggaat ttgcggatcg ccgggcaatc gtgcgtatgg
     1081 atgaccagac ctttatcctt gttgatgaaa ccgaggatgg ggtcgcccgg gatcggccgg
     1141 caacactgcg cgaactgcac ggccatgcct tcggtgccgc ggatcgtgat ggtccccagc
     1201 ggcttgctgg tgtgcttggc atgacgttcg ccctgctcgc cagccatcgc catcaactgg
     1261 tgcgccacca tgacgttgag gcgcttgccg agaccgatgt cggtgaggat ctcctctttg
     1321 ctcttggcgc cgtaatcgcg taacagcttc tgccagctct cgtcagtgat cagtgagata
     1381 tcggcatgca gggcccgtag cgcctggttc agcatgcgct cgccaagata agccgattcg
     1441 gtggtctgca tggatttgag gaagtgccgg atgtgcgcac gcgccttgcc cgagaccaca
     1501 tagttgagcc atgcagggtt ggggtgggca tgggcggccg tgatgatctc gacatggtcg
     1561 ccgttatgca gctcggtccg caacggcgcc agctcctggt tgatcttgac cgcgacgcag
     1621 cggttaccga tatcggtatg cacggcatag gcgaaatcga cggccgtggc gcccttgggc
     1681 agcgccatga tgttgccctt gggcgtgaat acgtagactt cgtccgggaa caggtcgacc
     1741 ttgaaatgct cgaggaactc gagcgaatcg gcgctctcgc tctggatctc gagcaggcgc
     1801 tgtagccaaa ggtgggtctg ctgctgcagc gcggtcaggt gcgcatcggt ggtcttgtac
     1861 agccagtggg cggcgacgcc cgcttcggcg atattgtgca tctcctgact acgcagctgc
     1921 acctcgatcg gggtaccgaa gggtccgaac aaggtggtat gcagggactg gtagccattg
     1981 gccttgggaa tggcgatgta atccttgaac ttgccgggga tcggcttgta aagcccatgc
     2041 agcgcgccga gcgcgaggta gcaggtcggc atgtccttga cgatgacacg gaagccatag
     2101 atgtcataga tctgcgagaa ggtcgtggtc ttgccggtca tcttcttgta gatgctatag
     2161 agatgcttct cgcgccccgt gacatcggcg tcgatatgca tctcctgcag gcgctgcttg
     2221 atcgcctcaa ggatcttgcc taccacctcc ttgcggttgc cgcgtgccgc cttgatcgcc
     2281 ttggcgatga cgcgatagcg catgggatag agatacttga aactcaggtc ttccagctct
     2341 tgaaagatgg cgttgaggcc gaggcgattg gcgatcggcg catagatatc gagcgtctcg
     2401 cgcgagatgc gccggcgctt ctctgcgctc atggcatcca tggtctgcat gttgtgcagg
     2461 cggtctgcca gcttgaccag gatcacgcgc acatcctgag acatcgccag aagcatcttg
     2521 cggaagttct ctgcctgcgc ctgggtagcg ctttggaact cgatcttgtc gagcttggag
     2581 agcccatcga ccagctccgc aacctgcttg ccgaactggt cggcgacttc ctgattggtg
     2641 acgccggtat cctcgacgac gtcgtgcagc agcgcagcga ccagggtcgg cgtatccata
     2701 tgcaattcgg ccaggataca ggccacggaa accggatggg tgatgtaagg ctcgccgctc
     2761 ttgcgcatct ggccctcgtg ggccgcggcg ctgaaacgat aggccgccca gacctgggcg
     2821 atgtcttcag gcttgaggta ttccttgagg cgcagggtga gtctggaatc ctcgctgtct
     2881 gccggcagca agggtggagc ttcggacttt acggcatctg ccgggcgttt ggcagtggtt
     2941 ctggacatga ggcgtcagct ggggatcggt ccaaccacgg catattgagc acaagggaca
     3001 tgccgccttt ggaccgctgc aggatcaggc gtggccgcga ttcaggactt caaggccgac
     3061 cttgccggct gcgatctcgc gcaaggcaag tacggtggat ttgtcctttt ggccgtgggt
     3121 attgaccatc ggctcggaac cgttcgccag ctggcgcgcg cggtaggcgg cgaccagggt
     3181 cagctgaaag cggttaggga tcttgtccag acaatcgtca acggtgatgc gtgccatctt
     3241 tacctccagg gttttcaggt gctgagcggc tcagcgtaag aatcgaacag gtgaatcaga
     3301 gcaatgtttc gagcagtgcg gcatggtcgc gcagctgcac ttcgcgcttc aggcgctgtg
     3361 cgcgaatgat ggccagcagg tcgtgcag
//
LOCUS       DATMTW010000163         1771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_81589, whole genome shotgun sequence.
ACCESSION   DATMTW010000163 DATMTW010000000
VERSION     DATMTW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81589"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VN084_03035"
     CDS             complement(<1..309)
                     /locus_tag="VN084_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HWT28462.1"
                     /translation="MNKNLLTIALAAMLAPALAPTLAFAEAGDWVIRARAVSVSPNES
                     SDLGKYVNKNVAAGAMSPGAELEVDSNVIPEIDVSYYFTKHLATELILATGTRHDVSI
                     H"
     gene            509..1765
                     /locus_tag="VN084_03040"
     CDS             509..1765
                     /locus_tag="VN084_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWT28463.1"
                     /translation="MIEFPVEDLAILKACAMSLAIGLLIGLERERSPAAKAGLRTFAL
                     TALFGTLLAILAARADAAWLLAVGMLSVAGLIIAAYLGEAGDGDDRGTTTQVALLLCF
                     VYGAMIGHGEGAIVVMLAIVTTMLLYFKPELHGLSKRMERRDMLSILQFGVLSCIILP
                     ILPDQGYGPYQVLNPQNIWLMVVLLSGISLAGYIALRLIGTRYGAPLVGIMGGLVSST
                     ATTLIYARHSRTQKGVFDLTVQVILLANLVVLIRIGVICATVSPQTFLQILPLLAGGL
                     LLGLPMAFRTWRRLGSQGEAPMPEVQNPAEIRASVLIGLIYAVVLVLSALLSDHAGSL
                     GLYAVALVSGLTDVDAISLSSLRLHNLGQIQATQAVMAIGLALITNMLFKFGLVVVIG
                     GKPLARQCLPGMAAIVVGMIAGLLLV"
BASE COUNT          290 a          515 c          578 g          388 t
ORIGIN      
        1 gtggatactg acatcgtggc gtgtgccggt ggccaggatg agttcggtgg cgaggtgttt
       61 ggtgaaatag taggacacat cgatctcggg aatgacgttg ctatcgactt cgagctcagc
      121 gccgggactc atggcgcccg cagcgacgtt cttgttgacg tatttgccga gatcgctgct
      181 ctcgttcggc gatacgctga cggcacgggc gcggatgacc cagtcgccag cttcggcgaa
      241 ggccagtgtc ggcgcaaggg cgggagccag catggctgct agcgcgatcg tgagtaagtt
      301 cttgttcatg attgtcctct ctgggctaat ggttatttga gtcgtcgaga ggattctagg
      361 gacgggcatg aacggctggt ttgatttggg tcaaacttcg ctgcaaatgt ccgccggggc
      421 agcaaatgac gcatgaacgg cttcgggcag atggcctgtt gggatgcaat cgtcttacaa
      481 tgcatcaaga catcagcgga ggcggcacat gatcgaattc ccggtagaag atctggcgat
      541 cctcaaggca tgtgccatga gccttgccat cgggttgttg atcggccttg agcgggagcg
      601 cagcccggcg gcgaaagcgg gcctgcgaac cttcgctttg acggccttgt tcggcactct
      661 gctcgccatc ctggcggcac gcgcggatgc cgcctggctg ctggcggtgg ggatgctgtc
      721 ggtcgcaggg ctcatcatcg cggcttatct cggcgaagcg ggcgacggcg atgaccgagg
      781 cacgacgacg caagtggcgc tgctgctgtg cttcgtctat ggcgccatga tcggccatgg
      841 tgaaggcgcc atcgtcgtca tgctggctat cgtcaccacc atgctgctgt atttcaaacc
      901 tgagctgcac ggcctcagca agcgcatgga gcggcgggac atgttgtcca tcctacagtt
      961 cggcgtgctg agctgcatca tcctgcccat cctgccggac caggggtatg gtccttacca
     1021 ggtgctcaac ccgcagaaca tctggctcat ggtggtgctg ctgtccggca tcagtctcgc
     1081 cggctacatc gcgttgcgcc tcatcggcac gcgctacggc gcaccattgg tcggcatcat
     1141 gggcggtctt gtctcgagca cggcgacgac cctgatctac gcgcgacaca gccgcacgca
     1201 gaaaggcgtg ttcgacctca cggtacaagt catcctgctc gccaatctgg tggtgctgat
     1261 ccgtatcggc gtcatctgcg ccaccgtctc gccacagacc ttcctgcaga tattgccgct
     1321 gctcgccggc gggctgctcc tggggctgcc gatggcgttc cgcacctggc gtcgattggg
     1381 cagtcaggga gaagcgccga tgccggaagt gcagaatccg gccgagatcc gcgcttcggt
     1441 gctgattggc ctgatctatg ccgtggtgct ggtccttagc gcgctgttgt ccgatcatgc
     1501 cggcagcctt ggtctctacg ccgtggcgct ggtctcgggg ctgactgatg tcgatgccat
     1561 ctcgctctcc agcctgcgcc tgcataatct cggccagata caggcgaccc aggcggtcat
     1621 ggccatcggg ctcgcgttga tcaccaacat gctgttcaag ttcggcctgg tcgtcgtcat
     1681 aggcggcaag cccctggcga gacagtgtct gccgggcatg gccgcgatcg tggtcggcat
     1741 gatcgccggg ttgctgctgg tctgatatct g
//
LOCUS       DATMTW010000164         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_315783, whole genome shotgun sequence.
ACCESSION   DATMTW010000164 DATMTW010000000
VERSION     DATMTW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315783"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VN084_03045"
     CDS             <1..290
                     /locus_tag="VN084_03045"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWT28464.1"
                     /translation="IEYPGVETMSVAVMGCVVNGPGESKLANIGISLPGTGEVPVAPV
                     FVDGQKTVTLKGDRIAEEFQAIVENYVREHYAEGGVLRASIPSKTIPLKAI"
     gene            305..1579
                     /gene="hisS"
                     /locus_tag="VN084_03050"
     CDS             305..1579
                     /gene="hisS"
                     /locus_tag="VN084_03050"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWT28465.1"
                     /translation="MSDQFQGTKFQSIKGFYDILPEQTALWQKLESTASRVLAQYGYR
                     NIRLPIVEPTDLFVRSVGEHTDIVEKEMYSWEDKLNGDRLTLRPEGTAGCVRAVVEHS
                     LTYNGPQRLWYMGPMFRHENVQKGRQRQFHQIGVESFGFDGPDVDAEQIILLARLWRE
                     LGLQDVALHLNTIGDASERATYRQVLIRHFEAHADLLDEDAKRRLHTNPLRILDSKNP
                     RMQAMIEAAPRLLDVLGEESRSHFDGLCQRLRDAGVAFQINPRLVRGLDYYNRTVFEW
                     VTTRLGSQGTIAGGGRYDSLVERIGGDATPACGFGIGLERVFLLMQEYGIEASEQPDV
                     YLVNVGELAEAQALAIAEHLRDAGLTVVMHAGGGSFKSQMKKADRSKARYAVILGEDE
                     VRTGVVSLKPMLVAGEQQKQDLESAVKLIQHG"
     gene            1572..2423
                     /locus_tag="VN084_03055"
     CDS             1572..2423
                     /locus_tag="VN084_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWT28466.1"
                     /translation="MAELLRIEGLTIVPAAMPERILVKDVTLTLQPGRTTCVVGESGS
                     GKSLTALAVMQLLSKQLKQVSGKVMFQGQDLVTLDNEQMRDIRGKRIGMIFQEPMTSL
                     NPVLTVGYQIGESLTVHLGLKGKALKARVASLLEQVGIPASRADSYPDELSGGQRQRV
                     MIAMSIACEPAMLIADEPTTALDVTVQAQILKLLDELKTRMNMGMLFITHDFGVVADI
                     ADDVVVMFRGEIVESGPVAEVLQRPRHPYTQALLACVPDADGKKALQPIDYAWVDGVD
                     DGVEAVV"
     gene            2420..>2879
                     /locus_tag="VN084_03060"
     CDS             2420..>2879
                     /locus_tag="VN084_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT28467.1"
                     /translation="MSEIILSARHLHKTYTQRSGKLGLTSTRIHALDDVSIDLHAGTT
                     LAVVGESGSGKSTLARCLLQLLPLDSGEVLFQGIDLAKLTAAELRQRRRDMQMVFQDP
                     FASLNPRMRIGEIVAEGLLIHGLGNADERRQKALAMLKRVGLSEADMNKYP"
BASE COUNT          613 a          823 c          892 g          551 t
ORIGIN      
        1 gtattgagta cccaggcgtc gagaccatga gcgtcgcggt gatggggtgc gtcgtcaacg
       61 ggcccggcga atccaaactc gccaatatcg gcattagttt gccaggcacc ggcgaagtgc
      121 cggttgcgcc agtgttcgtc gatgggcaaa agaccgtgac actgaagggc gaccgcatcg
      181 ccgaggagtt ccaggccatc gtcgaaaact acgtgcgcga acactatgcg gaaggcggcg
      241 tcctgcgtgc gtcgataccc tcgaagacca tcccgctaaa agcgatctga caataagcac
      301 cataatgagc gatcaattcc aaggcaccaa attccagagc atcaagggtt tctacgatat
      361 cctgcccgag cagactgcgc tctggcagaa gctggaatcc acggctagtc gtgtgcttgc
      421 acagtacggt taccgcaata tccggttgcc gatcgtcgag ccgaccgatc tattcgtgcg
      481 cagcgtcggc gagcataccg acatcgtcga gaaggagatg tactcatggg aagacaagct
      541 caacggtgac cgcctgacat tgcgtccgga aggcacggca ggttgcgtgc gtgcggtggt
      601 cgagcacagc ctgacctata atggcccgca gcgcctgtgg tacatgggcc cgatgttccg
      661 tcacgagaac gtgcaaaagg gtcgccaacg tcagttccat cagatcggtg tcgagtcgtt
      721 cggtttcgac ggcccggatg tcgatgccga gcagatcatc ctgctggctc gcctctggcg
      781 ggagctgggt ttgcaagacg tcgcgctaca cctcaatacc atcggcgacg ccagcgagcg
      841 agccacgtat cgtcaggtgc tcatccggca tttcgaagcc cacgccgacc tgctggatga
      901 ggatgccaag cgtcgcctgc ataccaaccc cctgcgcatc ctggacagca agaacccacg
      961 catgcaagcc atgatcgagg ccgcgccacg actgctcgat gtgctgggcg aagagagccg
     1021 gtcgcatttc gatggcctct gtcagcgatt gcgtgatgca ggagtggcgt tccagatcaa
     1081 tccacgcctc gtgcgcggcc ttgattacta caaccgcacg gtgttcgaat gggtcacgac
     1141 gcggctcggt agccagggca ccatcgccgg cggcggtcgc tacgacagcc tggtcgaacg
     1201 catcggcggc gacgccacgc cagcctgcgg atttggtatc ggacttgagc gcgtattcct
     1261 gctgatgcag gaatacggca tcgaagcaag tgaacaaccg gatgtttatc tggtcaatgt
     1321 cggtgaactg gccgaagcgc aggcgctggc gatcgccgag caccttcgtg acgccgggct
     1381 caccgtcgtc atgcatgcgg gcggcggaag cttcaagtcg caaatgaaaa aggcagaccg
     1441 cagcaaggcg cggtatgccg tcatcttggg cgaagatgaa gtacgaactg gggtcgtcag
     1501 cttgaaaccg atgctggtcg ctggcgaaca acaaaaacaa gatctggagt ctgcggttaa
     1561 actgattcaa catggctgag ctacttcgta tcgaaggtct gaccatcgtc cctgctgcga
     1621 tgcctgagcg catcctggtc aaggatgtca cgcttacgct gcaacccggc cgaaccacat
     1681 gtgtggtcgg ggagtccggc agcggcaaaa gcctgacggc gctcgccgtg atgcaattgc
     1741 tttccaagca gttgaagcag gtcagcggca aggtgatgtt ccaggggcag gacctagtca
     1801 ccctcgataa cgaacagatg cgcgatatcc gcggcaagcg catcggcatg atcttccagg
     1861 aaccgatgac ctccctcaac ccggtcctta ccgtgggcta ccagatcggc gagagcctga
     1921 cggtgcatct tggtctgaag ggcaaggcgc tgaaggcgag ggttgccagc ttgctggagc
     1981 aggtgggtat ccccgcgagc cgtgccgaca gttatccgga tgaactctcc ggagggcaac
     2041 gtcagcgcgt catgatcgcg atgagcatcg cttgcgaacc cgccatgttg atcgccgacg
     2101 agcccaccac tgcgctcgat gtgaccgtgc aggcgcagat cctcaagctt ctcgatgaat
     2161 tgaagacccg catgaacatg ggcatgctgt tcatcaccca tgatttcggt gtggtcgcgg
     2221 atatcgcgga tgatgtcgtc gtcatgttcc gcggcgagat cgtcgagtcc gggccggtgg
     2281 cggaggtgct acaacgccca cgccatcctt acacccaggc cttgcttgcc tgtgtgcccg
     2341 atgcggacgg taagaaagcc ttgcaaccga tcgactatgc ctgggtcgac ggtgtggatg
     2401 atggcgtgga ggcggtggta tgagcgagat cattctcagc gcccgacacc tgcacaagac
     2461 ctatacccag cgcagtggga aactcgggct gactagtacc aggatccatg cgctcgacga
     2521 tgtcagtatc gatctacatg cgggtacgac tctggctgtg gtgggcgagt cgggaagcgg
     2581 caagtctacg ctggcccgtt gtctgctaca attgctgccc ttggacagcg gcgaagtgct
     2641 tttccagggg atcgacctgg caaagttgac ggccgcggag ttaaggcagc ggcgacgcga
     2701 tatgcagatg gtgttccagg atcctttcgc ctcgctcaat ccacgcatgc gcatcggcga
     2761 gatcgttgcc gaaggcttgc tgatccatgg gctgggcaat gctgacgaac gtcggcaaaa
     2821 ggcgctcgcg atgcttaaac gcgtcggttt gagcgaagcc gatatgaaca aatatcccc
//
LOCUS       DATMTW010000165         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_382844, whole genome shotgun sequence.
ACCESSION   DATMTW010000165 DATMTW010000000
VERSION     DATMTW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382844"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1238)
                     /locus_tag="VN084_03065"
     CDS             complement(102..1238)
                     /locus_tag="VN084_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWT28468.1"
                     /translation="MDQGQAEKFVFDLLRMMMAKKASDLFITAGFPPAMKIDGKMTPV
                     TNQPLTSQHAKEIARSIMNDKQASEFDATKECNFAIGLPGVARFRVNAFVQRGATGLV
                     FRTITTKIPKFEDLGLPEVLKEVSMTKRGLVILVGGTGSGKSTTLAAMIGYRNENSYG
                     HIITIEDPVEFVHDHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETM
                     DYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERRHQLLMDLSLNVKAFVSQRLI
                     PKKEGKGRAAAMEIMLNSPLISDLIFKGDVHEIKEIISKSRELGMQTFDQALFDLFET
                     DQITYEDALRNADSVNDLRLKIKLESKHAKDKDLSSGLENLGIV"
     gene            complement(1277..2320)
                     /locus_tag="VN084_03070"
     CDS             complement(1277..2320)
                     /locus_tag="VN084_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWT28469.1"
                     /translation="MDISDLLAFSVKNKASDLHLSAGMPPLIRVHGDVRKINVPAMDH
                     TQVHDMVYDIMNDGQRKVYEETLECDFSFEIPNLARFRVNAFNQNRGVGAVFRTIPSK
                     VLTMEQLGCPPIFKEIAQNPRGICLVTGPTGSGKSTTLAAMVDYINENEMGHILTVED
                     PIEFVHVSKKCLVNQREVGPHTLSFSNALRAALREDPDVILVGEMRDLETIRLALSAA
                     ETGHLVFGTLHTSSAAKTVDRIVDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQG
                     RVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQNLTDLVRRNIISVGE
                     ARGKAANKDAFGG"
     gene            2365..3066
                     /locus_tag="VN084_03075"
     CDS             2365..3066
                     /locus_tag="VN084_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWT28470.1"
                     /translation="MTTIAERLQAVKARIHAAEHNAGRAADSVSPLAVSKAQPAGSLR
                     EAYAAGQRLFGENYLQEALDKQAALADLAIEWHFIGPIQSNKTQQIARHFSWVHGVDR
                     LKIAERLSNARPEDSAPLAICLQVNISQEASKSGVMPQELDALADAVQRLPRLRLRGL
                     MAIPAPTSDIELQHKQFRMVRELYSRLLAKGHALDTLSMGMSEDFQTAILEGATIIRI
                     GSAIFGPRAARITQS"
     gene            3081..>3451
                     /locus_tag="VN084_03080"
     CDS             3081..>3451
                     /locus_tag="VN084_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWT28471.1"
                     /translation="MKISFIGGGNMARALISGMLRQGFDAAAISVVDPDADKRASLQQ
                     DFGVSTTAQLPSVSAADVIVLAVKPQQLRDLSIFLGSLLQHQLVISIAAGIRCNDLAR
                     WLGGYAAIVRVMPNTPAQVQRG"
BASE COUNT          687 a         1003 c         1018 g          743 t
ORIGIN      
        1 ctcgtccggt agttcgccac cgagacattg gccagaatac acaacccatt gctgtagtcg
       61 ctcagctgtc attgcaatga caaaatcccc cgaaatcgcc gctaaacgat accaagattc
      121 tccagcccgg aggacaagtc cttgtccttg gcatgtttgc tttccagctt gatcttgaga
      181 cgcaggtcgt tgacagagtc cgcgttgcgt agcgcgtctt cataagtgat ctggtcggtc
      241 tcgaacaagt cgaacaaggc ctggtcgaaa gtctgcatac cgagctcacg cgatttggag
      301 atgatctcct tgatctcgtg cacatcgcct ttgaagataa ggtccgagat cagcggcgaa
      361 ttgagcatga tctccatcgc cgctgcgcgt cccttgcctt ccttttttgg gatcaggcgc
      421 tgcgagacga aggccttgac gttgagtgag aggtccatca gcagctggtg gcgacgttct
      481 tcagggaaga agttgatgat gcggtcgagc gcctggttgg tgctgttggc atgcagtgtc
      541 gccatacaaa ggtggccggt ctcggcgaac gcgatggcgt agtccatggt ctcgcggtcg
      601 cggatctcgc cgatgaggat cacgtccggt gcctggcgca aggtgttctt caatgcggcg
      661 aaccagtttt cggtgtcaac accgacctcg cgctgcgtga tgatgcagtt cttgtggtca
      721 tgcacgaact ctacgggatc ttcgatggtg atgatgtggc cgtaactatt ctcgttgcgg
      781 tagccgatca tggccgccag tgtggtcgat ttaccggatc cagtaccgcc caccagaatt
      841 accaggccgc gcttggtcat cgacacctcc ttgagcactt cgggcaagcc gagatcctca
      901 aacttcggga ttttggtggt gatggtccgg aacaccaggc cggtagcccc gcgttgaacg
      961 aaagcattga cccggaagcg cgccacgccg ggcaggccga tggcgaaatt gcactcctta
     1021 gtggcgtcga actcggacgc ctgcttatcg ttcatgatcg aacgggcgat ctccttggca
     1081 tgctggctgg tcaagggttg attggtcacc ggcgtcatct tgccgtcgat cttcatcgcc
     1141 ggcggaaagc ctgccgtgat gaaaaggtcg gacgccttct tcgccatcat catgcgcaat
     1201 aaatcgaata cgaatttctc tgcctgtccc tgatccatgg ttatgccttg ttatgctgct
     1261 cgttcagtat tgtgggtcag ccgccgaaag cgtctttatt ggcagccttg ccacgcgctt
     1321 cgccgaccga gatgatgttg cggcggacga ggtcggtgag gttctgatcc agggtctgca
     1381 tgccgacgtt ctggccggtc tggatggccg aatacatctg tgcgatcttg ttctcgcgga
     1441 tcaggttacg aatggccggc gtaccgatca tgatctcgtg ggccgcgaca cggccttgct
     1501 cgtccttggt cttgcacaag gtctgtgaga tgaccgcacg cagtgattcc gagagcatgg
     1561 aacgcaccat ttccttttcc gcggcgggga acacgtcgac gatacggtcc acggtcttcg
     1621 ccgcagaact ggtatgcagc gtaccaaaca ccaagtggcc ggtttcggct gcggacaacg
     1681 ccaggcggat ggtctccaga tcgcgcattt caccgaccag gatcacgtcc gggtcttcac
     1741 gaagcgcagc gcgcagcgcg ttgctaaagg acaatgtgtg ggggccgacc tcacgctggt
     1801 tcaccaggca cttcttggac acgtgtacga attcgatcgg atcctcgacg gtgaggatat
     1861 ggcccatctc gttctcgttg atgtaatcga ccatcgccgc cagcgtcgtc gatttgcctg
     1921 aaccggtggg gcctgtcacc agacagatgc cgcgcgggtt ctgcgcgatc tccttgaaga
     1981 tcggcgggca gccgagctgt tccatcgtca gcaccttgga tggaatggtc cggaacaccg
     2041 cgccgacacc ccggttctga ttgaaggcat tgacgcggaa acgcgccaga ttggggatct
     2101 cgaacgagaa gtcgcattcc agcgtctctt cgtagacctt gcgctgaccg tcgttcatga
     2161 tgtcgtacac catgtcgtgg acctgcgtat ggtccatggc ggggacgttg atcttgcgca
     2221 catcgccgtg cacgcggatc aagggcggca tcccggcgga aaggtgcagg tccgatgcct
     2281 tgtttttgac cgagaatgcc agcaaatcgg aaatatccac ggagccccct gttataattt
     2341 tttagtcatg ataatgagcg aaatatgacc acaattgccg aacgcttgca agctgtaaaa
     2401 gcgcgcatcc atgcggctga gcacaatgcc gggcgtgccg ccgacagtgt ttccccgctc
     2461 gcggtcagca aagcccagcc ggccggatca ctgcgcgaag catacgccgc cgggcagcgt
     2521 cttttcggcg agaattacct gcaggaagcg ctcgacaagc aggccgcgct cgccgatctc
     2581 gccatcgaat ggcactttat cgggcctatc cagagcaaca agacgcaaca gatcgcccgg
     2641 catttttcct gggtgcacgg cgtggataga ttgaagatcg cggagcggct aagtaacgca
     2701 cgcccggaag acagtgcccc gctcgcgatc tgcctgcaag tgaacatcag ccaggaggcg
     2761 agcaagagcg gcgtgatgcc gcaggagctt gatgcgctgg cagacgccgt gcagcgatta
     2821 ccaaggctac ggctacgggg gctgatggcc atcccggcgc cgacgagcga cattgaatta
     2881 cagcacaagc agtttagaat ggtgcgtgaa ctctattcac ggctgctcgc gaaaggtcac
     2941 gccctggata ccttgtcgat gggcatgtcc gaggatttcc agacggccat cttggagggc
     3001 gccaccatca tccgcatcgg ctctgccatt ttcggcccgc gcgccgcacg tataacgcaa
     3061 tcatgatcga caggaagcat atgaaaatca gtttcatcgg cggcggcaac atggcacgcg
     3121 cgctcatcag cggcatgctc cggcaagggt tcgatgccgc ggccatcagc gtcgtcgacc
     3181 ccgatgccga caagcgtgca tcgctgcagc aggacttcgg cgtctcaacg actgcacaat
     3241 tgcccagcgt cagcgctgcc gatgtcatcg tgctcgccgt caagccccaa caattacgcg
     3301 acctgtcgat ctttctcggt agcctgctgc agcatcagtt ggtcatctcc atcgccgctg
     3361 gcatccgctg caacgacctg gcacgctggc tgggcggtta tgccgccatc gtgcgcgtca
     3421 tgccgaatac cccggcgcag gtccagcgag g
//
LOCUS       DATMTW010000166         4060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_484152, whole genome shotgun sequence.
ACCESSION   DATMTW010000166 DATMTW010000000
VERSION     DATMTW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484152"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..769
                     /locus_tag="VN084_03085"
     CDS             56..769
                     /locus_tag="VN084_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HWT28472.1"
                     /translation="MSLSPEARKFLRSTHSGLLSTISARFEGYPYGSVAPFVLDHDGQ
                     PLILISTLADHTKNIIDDSKVSLLVFDASNTSAAEDLHVNARLTLLGDAEQTDKQDTL
                     LRQRYLRYLPSAEQYFEMHDFYFYRIHILQARYIAGFGKMSWLPGEELRSARSPLAAQ
                     EAGILDHMNEDHAESLLRYCAHVHGLTPATAEMIGIDSDGFDVRADGRILRFDFDQPI
                     HDAQGARAALVALSGQCRA"
     gene            766..1128
                     /locus_tag="VN084_03090"
     CDS             766..1128
                     /locus_tag="VN084_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HWT28473.1"
                     /translation="MIAALKRLSVLRIVAGSSLIALIMVCLAWEGWLAPLRPNGSLLI
                     LKAVPLLLPLYGIIHGRRYTYQWASMFILLYFTEGAVRAWADGGVAAKLALVEVVLSL
                     LFFTAAIFYARLSRNPGA"
     gene            1143..1886
                     /gene="surE"
                     /locus_tag="VN084_03095"
     CDS             1143..1886
                     /gene="surE"
                     /locus_tag="VN084_03095"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWT28474.1"
                     /translation="MRILLSNDDGYFAPGLNILAEHLSSLAEISVVAPERNRSGASNS
                     LTLDRPLTVRKAHNGFLYVNGTPTDCVHLALTGLMSSPPDMVISGINDGANMGDDTIY
                     SGTVAAAMEGYLLGIPSIAVSMSRHGAQYFDTAAKVVIDLVERYKKTGFPPPLLLNVN
                     VPDIPYDELHGMAVTRLGKRHKAEPVIKTSTPRGETVYWVGAAGNAQDAGPGTDFHAV
                     EHEQVSLTPLQIDLTQFKQLDAVEDWLTR"
     gene            1883..2539
                     /locus_tag="VN084_03100"
     CDS             1883..2539
                     /locus_tag="VN084_03100"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWT28475.1"
                     /translation="MSTALRGIGMTSQRTRERMLARLREQGVRDEVVLAAMNTIPRHI
                     FLDEALSIRAYEDVSLPIGFGQTISQPYIVARMTEILRDGRSLNKVLEIGTGCGYQTA
                     ILASVAKEVYSVERIRPLLFKARDHLRKLRLSNIKLQHADGGMGLPELAPFDGIMVTA
                     AASHVPQELLDQLAIGGRMVIPVGTEEQFLYLIQRTAKDFKKTKLEAVRFVPLLGGTN
                     "
     gene            2629..3549
                     /locus_tag="VN084_03105"
     CDS             2629..3549
                     /locus_tag="VN084_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWT28476.1"
                     /translation="MPHASTATKPGAKTVAKDWRPDVYTVKKGDTLYSIGLEFGYDYK
                     DLAQANNIPPPYVIRVGQQLRLKEGKPAAPTVAKAPAESGVVVTPLNTEGSTTTEAKP
                     SGPVASSTPIAGITPILSEPKALRVPYSEQALLTPPPGPNAASATAAKSTDPAKPVEQ
                     AKVDVTKPSTPMKPDAMPGDDDVIEWAWPANGKLVSGFSEAAGGKGIDIAGSEGQAVM
                     AAAPGKVIYSGSDLRGYGKLVIIKHNKTYLSVYAHNSNIVVKEGQFVSRGQKIAEMGS
                     SDADRVKLHFEIRQQGKSVDPAKYLAGNPG"
     gene            3626..3952
                     /locus_tag="VN084_03110"
     CDS             3626..3952
                     /locus_tag="VN084_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28477.1"
                     /translation="MQKNTKPDSLFETIMATFRMGLCLLGIIGLSVETFRDEGWLKRA
                     LGKLFTSDTALIAISLIFIGLFLVNRLLSAPTNDKNRWIGDLPLYLMMGIGAFFLFKL
                     VTTGGF"
BASE COUNT          813 a         1356 c         1137 g          754 t
ORIGIN      
        1 agctctgcat cgcgctcggc aagcgcctcg ccgagaccgc ccagaagttg tcggcatgag
       61 cctctcgcca gaggcacgca agttcctgcg cagcacccat agcgggctgc tctcgaccat
      121 ttccgcgcga ttcgagggtt acccttacgg ctcggtcgcg ccattcgtgc tggaccatga
      181 tgggcagccg ttgatcctca tcagcacgct ggctgaccat accaagaaca tcatcgacga
      241 cagcaaggtc tcgctgctgg tattcgacgc ttcgaacacc agcgccgccg aagacttgca
      301 cgtcaacgcg cgcctgaccc tgctcggcga cgccgaacag accgacaagc aagacacctt
      361 gctgcgccag cgctacctgc gttatctgcc gagcgccgag cagtacttcg agatgcacga
      421 tttctatttc tatcgtatcc atatcctcca ggcacgctat atcgccggct tcggcaagat
      481 gtcatggctt cccggtgagg aactgcgctc ggcacgctcc ccgctcgcag cgcaggaagc
      541 cggcatcctg gaccacatga atgaggacca cgccgagagc ctgttgcgct attgcgccca
      601 tgtgcatggg ctgacaccgg caacggccga gatgatcggc atcgatagcg acggtttcga
      661 cgtgcgtgcc gacggtcgca tcctgcgctt cgatttcgac caaccgatcc acgatgcgca
      721 aggcgcccgg gcggcactgg tcgcgctttc tggccaatgt cgcgcatgat cgccgctctc
      781 aagcgactga gtgtgctgcg catcgtcgcc ggcagcagcc tcatcgcgct catcatggtc
      841 tgtcttgcct gggaaggctg gctcgccccg ctcagaccca acggctcgct gctgatcctc
      901 aaggccgtac ccctgctgct gccgctctat ggcatcatcc acggacgccg ctacacctat
      961 cagtgggcca gcatgttcat cctgctctat ttcactgaag gcgccgtccg cgcctgggct
     1021 gatggcggtg tggcagcgaa gctcgccctg gtggaggtgg tgttgagcct gctgttcttc
     1081 accgccgcga ttttttacgc ccgccttagc cgaaatcctg gggcataact tataatttat
     1141 gcatgcgcat cctactttcc aacgatgacg gctattttgc gccggggctc aatatccttg
     1201 ccgaacacct gtcatccctc gcagagatct cagtcgtcgc accggaacgc aatcgcagtg
     1261 gcgccagcaa ttcgttgacg ctggaccgac cgctcaccgt gcgcaaggcg cataacggct
     1321 tcctctatgt caacggcacg cctacggact gcgtccacct ggcgttgact ggactcatga
     1381 gcagcccgcc ggacatggtg atctccggca tcaacgacgg cgccaatatg ggcgacgaca
     1441 ccatctactc gggcaccgtg gccgccgcca tggaaggtta cctgctgggc atcccctcga
     1501 tcgctgtctc catgtcccgg catggtgcgc aatatttcga cacggcggcc aaggtcgtca
     1561 tcgatctggt cgagcgttac aagaagaccg gcttcccgcc gccactgcta ctcaacgtca
     1621 acgtgccgga tatcccctat gacgaattgc acggcatggc ggtcacccgc ctcggcaaac
     1681 ggcacaaggc cgagcccgtg atcaagacca gcacaccgcg tggcgagacc gtatattggg
     1741 tgggcgccgc cggcaatgcg caggacgcag gccccggcac cgatttccat gcggttgagc
     1801 atgagcaggt atcgctgacg ccgctgcaga tcgatctcac gcaattcaaa cagctggatg
     1861 cggttgagga ctggctgacg agatgagtac ggcactgcgc ggtatcggca tgacttcaca
     1921 acgcacgcgc gagcgcatgc tggcgcgtct gcgggaacaa ggcgtgcgcg acgaagtcgt
     1981 acttgctgcg atgaacacca ttccccggca tatcttcctt gacgaggccc tgtcgatccg
     2041 cgcttacgag gatgtctcct tgccgatcgg tttcggccag accatctcgc aaccctacat
     2101 cgtcgcccgc atgaccgaga tcctgcgcga cggccgcagc ctcaacaagg tactggagat
     2161 cggcaccggc tgcggctacc agaccgccat cctggccagc gtcgccaagg aggtctattc
     2221 cgtcgagcgc atccggccct tgctgttcaa ggcacgcgac cacctccgca agttacgcct
     2281 gtccaacatc aagctgcagc acgccgatgg cggcatgggc ctgccagaat tggcgccttt
     2341 cgacggcatc atggtcacgg ctgcggccag ccatgtgcca caggagctac tcgaccagct
     2401 cgccatcggc ggccgcatgg tgatcccggt cggcacggaa gaacagttcc tctacctgat
     2461 ccaacgcacg gccaaggatt tcaagaaaac caagctcgaa gccgtcaggt tcgtgccttt
     2521 gctgggtggt accaattgat cagaacgtta ggcctcatcc tgctggcaag tcttatcctt
     2581 agcgcttgta gcagtgcaca aaaagccccc gtcatcgatc gcgcccctgt gccgcacgct
     2641 tcgacggcga ccaagcccgg cgccaaaacc gtcgccaagg actggcgccc ggatgtctat
     2701 accgtgaaga agggcgacac cctctacagc atcggcctcg agttcggtta tgactacaag
     2761 gacctcgcgc aggccaacaa catcccaccg ccatacgtca tccgtgtcgg ccagcaactg
     2821 cgcctgaagg aaggcaagcc tgctgcaccc actgtcgcca aagcccctgc cgagtctggc
     2881 gtggtggtga caccgctcaa taccgaaggc agcaccacga ccgaggccaa gccctccggg
     2941 ccggtggcca gcagcacgcc gatcgccggg atcacgccca tattgtccga accgaaggcg
     3001 ctgcgtgtgc cttacagtga acaggcgctg ctgacgccgc cgcctggccc caatgcggct
     3061 tccgccaccg cggcaaaatc gacggacccg gccaaaccgg tcgaacaggc caaggtcgat
     3121 gtcaccaaac cttccacacc gatgaaaccg gacgccatgc ccggcgacga tgatgtcatc
     3181 gaatgggcct ggcccgccaa cggcaagctg gtatcaggct tcagcgaagc cgctggcggc
     3241 aagggcatcg atatcgccgg cagcgaaggc caggcggtga tggcagccgc gcccggcaag
     3301 gtcatctaca gcggttcgga tctgcgcggc tacggcaaac tggtgatcat caagcacaac
     3361 aagacctatc tctcggtcta cgcccacaac agcaacatcg tggtgaaaga gggccagttc
     3421 gtcagccgcg gccagaagat cgccgagatg ggcagctccg acgccgaccg ggtgaaactg
     3481 catttcgaga tccgccagca aggcaagtcg gtcgatccgg ccaaatacct ggctggcaat
     3541 ccgggctgat tcattgcttg actgaccgtg gcatcgcgat taacttagcc gagcgtttaa
     3601 cccagccgca atacccgagg tcactatgca aaagaacacc aagccggatt ccctgttcga
     3661 aacgatcatg gcgacctttc gcatgggctt gtgcctgctc ggcatcatcg ggctttcggt
     3721 tgaaaccttt cgtgacgaag gctggctgaa gcgcgcactg ggcaagctgt tcacctccga
     3781 cacggcgctc atcgccatct cgttgatctt catcggcctt ttcctcgtca atcgcctgct
     3841 gtcggcaccc accaatgaca agaaccgatg gatcggcgac ctgccgctct acctgatgat
     3901 gggtatcggc gcattcttcc tgttcaagct cgtcaccacc ggcggcttct gagttcgccc
     3961 caagctgaac cattcgggca ccctccggcc caatcctcca tggaagaccc tacgctggaa
     4021 caacttggcg aactgcctgg cctcacgctg ctggaattcg
//
LOCUS       DATMTW010000167         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_282116, whole genome shotgun sequence.
ACCESSION   DATMTW010000167 DATMTW010000000
VERSION     DATMTW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282116"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VN084_03115"
     CDS             complement(<1..307)
                     /locus_tag="VN084_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28478.1"
                     /translation="MSQSNEQHEVPGRLQRWAQTLADGFENLSHGVRRTVQIGVLLIS
                     ALLALFIITVPFDLQSQCYFAAGCFVAALILRKQSGRFPVVLLIGLSLTASLRYMYWR
                     "
     gene            complement(304..1491)
                     /gene="bcsQ"
                     /locus_tag="VN084_03120"
     CDS             complement(304..1491)
                     /gene="bcsQ"
                     /locus_tag="VN084_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090540 - bacterial cellulose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HWT28479.1"
                     /translation="MSRFGESAASYIEMENSFNYKELPESPVPAASGAFVATEESAAA
                     IAPESVETVQPLSETMATPAHPLDRRVEPVPDDLLEATSVDAAVSEPVPVPANAGSLR
                     SLLAEVALERQAEARAQNEEALRRSIPNGSVAVTPARVIAVVSPKGGVGKSTLCAALA
                     GALGAKGRRLAIDLDPQNALQYHLGVSPDVAGMASASLTGECWSGLLLDGIGGTRVLP
                     FGPISGQERRALERFLEDDTHWLARQLARMDLAADDVVILDTPPGRTLYLEQVLDVAD
                     QVIVVITPDPASFMTLDPIDRLLEGRTHQAWRRDCHYVVNQFDASRTFCQDMLEVLKR
                     RLGKQLIGVLPLDHAISEGLAFGVNPLLEDEDSSARQEVLAIADALKSLVRTSALAGS
                     RAS"
BASE COUNT          369 a          518 c          509 g          309 t
ORIGIN      
        1 tccgccagta catgtagcgc agcgaagccg tcagcgacag accgatcagc agcaccaccg
       61 ggaatcggcc ggattgtttg cgcagtatca gcgcggcgac aaaacagcct gcggcgaaat
      121 agcactgtga ttggaggtcg aaaggcaccg tgataatgaa cagcgccaaa agagccgaga
      181 tcagcaggac accgatctgc acggtgcgcc gtaccccatg ggaaaggttc tcgaacccgt
      241 cggccagggt ttgcgcccag cgttgcaggc ggccgggcac ttcatgttgt tcgttggatt
      301 gactcatgat gcgcgactac ctgccaatgc agaggtccgg acaagtgact tcagcgcatc
      361 ggcaatggcc aggacttctt gccgtgcaga cgagtcttca tcttccagta atgggttgac
      421 gccaaaggcg aggccttcgc tgatggcgtg atcgagcggc aggacaccga tcaattgttt
      481 gcccaagcgg cgcttgagca cttcgagcat gtcctggcag aacgtgcgcg aggcatcgaa
      541 ctggttgacg acatagtgac agtcacgacg ccaggcctga tgggtacgcc cttcgagcaa
      601 tcgatcgatg ggatccagcg tcatgaacga ggccgggtcc ggcgtgatga cgacgatcac
      661 ctggtcggcg acatcgagca cctgctccag atacagggtg cggccgggcg gtgtgtcgag
      721 aatcaccaca tcgtcagccg ccagatccat gcgcgccaac tgccgcgcca gccaatgagt
      781 gtcatcctcg aggaaacgct cgagcgcacg acgctcctgc ccggagatcg ggccgaaagg
      841 caggacgcgg gttccgccga tgccatcgag cagcaagccg ctccagcatt cgccggtcag
      901 gctggcgctg gccatgcctg cgacatccgg actgacaccc aggtgatatt gcaacgcgtt
      961 ttgcggatcc agatcaatgg ccagcctgcg ccccttcgca ccgagcgcgc ccgccagcgc
     1021 cgcacagagc gtacttttac ccacgccgcc cttgggcgac accaccgcga tgacccgggc
     1081 cggagtgacc gctacagaac cgttcgggat cgatcggcgt aacgcctcct cgttctgcgc
     1141 acgcgcctcg gcctggcgct caagcgcgac ttcagccagc agggagcgca gggaaccagc
     1201 attggcgggg actggaacag gttcggatac cgccgcgtcg accgacgtgg cctcaagcag
     1261 atcatccggc accggctcga ctcgccggtc cagcggatga gcgggcgtcg ccatcgtctc
     1321 ggacagcggc tgcacggttt cgaccgactc gggagcaata gctgccgccg actcttccgt
     1381 cgccacgaaa gcgccagatg cagcaggtac cggagattca ggcaattcct tgtaattgaa
     1441 ggaattctcc atctcgatat agctggccgc actctctccg aacctgctca tcaggttgtc
     1501 aatatcattt gtccgattca taagcccttc acggcgaggt cagtagcatt cgggctcgta
     1561 taacggcaag cgagtgcatt cgcgtggcga aatgatatcg tcaatatttt gttaacaaca
     1621 agctctttta ttggatccaa tagaaaataa gacaggcgga cgccaaagta ttggcgccga
     1681 ctgtttcaca ggtttatcag cgagc
//
LOCUS       DATMTW010000168         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_350330, whole genome shotgun sequence.
ACCESSION   DATMTW010000168 DATMTW010000000
VERSION     DATMTW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350330"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..766
                     /gene="pgl"
                     /locus_tag="VN084_03125"
     CDS             56..766
                     /gene="pgl"
                     /locus_tag="VN084_03125"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWT28480.1"
                     /translation="MQKPIQTLTVGRQTSRWHSFESQAALNQASLARIVAAASEAIAA
                     RGAFGIVLAGGNTPKSIYELLRHADTDWSKWHIYYNDDRCLPVDHPDRNSLMAAQAWL
                     DHVAIPKQQIHDIPTELGPVAGAKAYAEILTGLGDFDLVLLGLGEDGHTASLFPGHTW
                     DDSVDAFPVFDAPKPPPERVSISGARLSRSREVIFFVTGAGKQEAVDNWRKSVAIPAT
                     VIAPEAGVDVYIYGVNID"
     gene            complement(830..1954)
                     /gene="apbC"
                     /locus_tag="VN084_03130"
     CDS             complement(830..1954)
                     /gene="apbC"
                     /locus_tag="VN084_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HWT28481.1"
                     /translation="MRYCATHQENRSMSVTESQVQTALKQVVDPNTGKDLITTKSARN
                     IQIQGNDVSVDIVLGYPAKSVMAEIRALVEAQLKTIAEIGTITINIGSRIAAHSVQRG
                     VGLLSGVKNIIAVASGKGGVGKSTTAVNLALALAAEGATVGILDADIYGPSQPQMLGI
                     SGRPESTDGKSIEPLQSHGIQAMSIGFLVDVDTPMVWRGPMVTGALEQLLRDTRWKDL
                     DYLVIDLPPGTGDIQLTLSQKVPVTGAIIVTTPQDIALLDARKGLKMFEKVGIPILGI
                     VENMSTHICSNCGHEEHIFGAGGGEAMCKDYNVELLGSLPLDIRIREQADGGKPTVIA
                     EPDSQISATYKQIARTTAIKIANLGLDHSSKFPNIVIQNT"
BASE COUNT          356 a          562 c          605 g          443 t
ORIGIN      
        1 ggttttttaa agcgactgaa gtccgtcatt gtactattac aaggcgttcc tgaacatgca
       61 aaaacctatc caaaccctta ctgtcggaag gcagaccagt cgttggcatt cttttgaatc
      121 ccaggcagca ctcaatcaag cctctttagc tcgtatcgtc gccgcggcca gcgaggcgat
      181 cgctgcccgc ggtgccttcg gcatcgtgct cgctggcggc aacaccccca agagtatcta
      241 cgaattattg cgtcatgccg acactgactg gagtaagtgg catatctact acaacgacga
      301 tcgctgccta ccggtcgatc atccggaccg caacagcctg atggccgcac aagcctggct
      361 cgatcatgtc gccatcccca agcagcagat ccacgatatc ccgaccgaac tcgggcccgt
      421 cgctggcgcg aaggcttatg ccgagatcct cacgggcctg ggcgattttg acctggtgct
      481 gctgggcctg ggcgaagacg ggcataccgc gagcttgttc cctggccaca cctgggatga
      541 cagcgtcgat gcatttccgg tgttcgacgc gccgaaacca ccgccggagc gggtctccat
      601 cagcggcgcg cgtctcagtc gttcgcgaga agtgatcttc ttcgtcaccg gcgccggcaa
      661 gcaggaggcg gtggacaact ggcgcaaaag cgtcgcgatc ccggccacgg tgatcgcgcc
      721 ggaagcgggc gtcgatgtct atatctatgg cgtgaacatc gactgatagc gtccatgaaa
      781 tgaaaacggc tcacaacatg tgagccgttt tttttaacat cgagcgtggt caggtgttct
      841 ggatcacgat attcgggaac ttgctgctgt ggtccagccc gaggttagcg atcttgatcg
      901 ccgtggtacg cgcgatctgt ttgtaggtcg cgctgatctg gctatccggc tcggcgatga
      961 cggtgggttt gccaccatcg gcttgttcgc ggatgcggat atcgagcggt aggctcccca
     1021 gtaattcgac attgtaatcc ttgcacatgg cttcgccgcc gcctgcgccg aagatgtgtt
     1081 cctcgtgacc gcagttggag cagatgtggg tgctcatgtt ctcgacgatg ccgaggatgg
     1141 ggatgccgac cttctcgaac attttcaatc ccttgcgcgc gtctagcagg gcgatgtcct
     1201 gcggcgtggt cacgatgatg gcgccggtga cagggacttt ctgcgacagc gtgagctgga
     1261 tgtcgccggt acctgggggt aggtcgatca ccaggtagtc gaggtccttc cagcgtgtat
     1321 cgcgcagcaa ttgctcaagt gcgccggtga ccatggggcc gcgccagacc atgggcgtgt
     1381 ccacgtcgac caggaagccg atggacatgg cctggatacc atggctttgc agcggctcga
     1441 tgcttttgcc gtccgtgctt tccggtcgtc cgctgatgcc tagcatctgc ggttgcgagg
     1501 ggccatagat gtcggcatcg aggatgccga cggtcgcgcc ttccgctgcc agcgccaacg
     1561 ccagattgac ggctgtggtg gacttgccga cgccgccctt gccggaagcg actgcgatga
     1621 tgttcttgac cccgctaagc aggcctacgc cacgctgtac gctgtgagcc gcgatgcggc
     1681 tgccgatatt gatcgtaatc gtgccgattt cggcgatggt cttgagctgc gcctcgacca
     1741 gcgcccggat ctcggccatg actgatttgg ccgggtagcc gagcacgata tcgacactga
     1801 catcgttgcc ctggatctgg atgttcctgg cggatttggt ggtgatcagg tccttgccgg
     1861 tattggggtc gacgacttgc ttaagggctg tttgaacctg cgattcggtg acggacatgc
     1921 ttcgattttc ctgatgggtg gcgcagtagc gcatgatttc gacaag
//
LOCUS       DATMTW010000169         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_282583, whole genome shotgun sequence.
ACCESSION   DATMTW010000169 DATMTW010000000
VERSION     DATMTW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282583"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1043)
                     /locus_tag="VN084_03135"
     CDS             complement(21..1043)
                     /locus_tag="VN084_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HWT28482.1"
                     /translation="MTILVTGTAGFIGNFLALRLLERGDTVIGVDNLNDYYDVRLKEA
                     RLARIQSHPGFTEVRADVHDREAMQALFATHKPRLVVHLAAQAGVRYSLINPQAYADA
                     NLQGFLNILEGCRQHEVQHLVFASSSSVYGANSKMPFSVHDNVDHPLSLYAATKKANE
                     LMAHSYSHLYRLPVTGLRFFTVYGPWGRPDMAYFLFAKAIMAGRTIDVFNHGEMQRDF
                     TYIDDIVEGVLRVLDHVPTPDPDWHGDAPDPGSSTAPYRIYNIGNSQPVPLMRLIELL
                     EQNLGRTAVKRFLPMQAGDVTATFADVEDLTRDVGFKPDTAIETGIARFVAWYREFYG
                     NQAPGV"
     gene            complement(1063..2502)
                     /locus_tag="VN084_03140"
     CDS             complement(1063..2502)
                     /locus_tag="VN084_03140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWT28483.1"
                     /translation="MTTPQVVPRYLVPATWLLVVLLLFWGLGDIPLLSYNEARRALPT
                     AAMFQSGDWLLPRLDGALYLAKPPLFYWLAAIAAHLVGSVSEWAMRLPSAFAALLAAV
                     LAFRFARRHFGILAALFTLQLLLANAGFALFARRAEIEMLLSTLCFASLLAALHFVYE
                     EGRRTWLWLSYALLGLAVLTKGPLALIFVTLPLLVLAICRPHPRLWLALRDPWGWLVF
                     LVVGSAWFVAVSYRLGPEIWLSTIHHDMMNKMYGHTGEPVYSYLLWLLADFFPAGLLI
                     LAWPLTRWRRWREQHGMAERIALVVLVPFLLYTAFSDKHAKYLLPLYPWIAILLGEQL
                     AQLCAAAKRWQRLTLLTASLLMLAGYSAYYAFAERQVFHYRYSALQQFQAWKATQGNV
                     PFYGFGHLDERLVFYAGQDIPILDVAALKRLQAAHTPAFVLYEHGPAQENLGLDCQVR
                     EFRPYLNRKKILTIHGLGAACQPLPHHPS"
     gene            complement(2499..>3001)
                     /locus_tag="VN084_03145"
     CDS             complement(2499..>3001)
                     /locus_tag="VN084_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWT28484.1"
                     /translation="QRPLLRIVFLDTNQDPRGQAEYLRKQFAAGGNPIWKIAVGHHPL
                     RTYGKHHQDEQATVDNALLSAMQQSGVDLYIAAHDHNQQLIVREGEPLQIVTGAGGAK
                     LYDLKSPGSDMHFGKAEHGFLRVALDASSLNFSFIDSQAKTRASYHWDRSCVAGLADC
                     LESAPQ"
BASE COUNT          648 a          886 c          945 g          522 t
ORIGIN      
        1 agcgttcaga catctggcga tcagacacct ggcgcttgat tcccatagaa ttcacggtac
       61 caggcgacga atcgggcgat gccggtctcg atcgcggtat caggcttgaa acccacatcc
      121 cgcgtgagat cctcgacatc ggcgaatgtg gccgtgacat cgcccgcctg catgggcagg
      181 aagcgtttga cggcggttcg gccgaggttt tgctcgagta actcgatcaa tcgcatgagc
      241 ggcaccggct ggctgttgcc gatattgtag atacggtaag gcgccgtcga actgccgggg
      301 tccggggcat cgccgtgcca gtcaggatcg ggcgtcggca cgtgatccag cacacgcagc
      361 acgccttcga cgatatcgtc gatataagtg aaatcgcgct gcatctcgcc atggttgaag
      421 acgtcgatgg tgcggcccgc catgatggct ttcgcgaaca ggaaatacgc catgtcgggc
      481 cggccccagg ggccgtatac ggtgaagaag cgcaggccgg tcaccggcaa ccggtaaaga
      541 tggctgtagc tgtgcgccat gagctcgttg gctttcttgg tcgcggcata gaggctcaac
      601 gggtggtcga cgttgtcatg cacggaaaac ggcatcttgc tattggcgcc gtagaccgag
      661 ctggacgaag cgaagaccag atgttgcacc tcgtgctggc ggcagccttc caggatattg
      721 aggaagccct gcagattggc atcggcatag gcttgcggat tgatcagcga atagcgcaca
      781 ccggcctgcg ccgcgagatg caccacaagc cggggcttgt gcgtggcaaa cagcgcttgc
      841 atggcttcgc ggtcatggac atcggcgcgc acttccgtga agcccggatg ggactggatg
      901 cgtgccagcc gagcctcttt cagacgcacg tcgtaataat cgttgaggtt gtcgacgccg
      961 atgacggtat ccccgcgctc cagcaggcgc aaggccagaa aattgccgat gaagccggcg
     1021 gtgccggtca cgaggatggt catggctgct ctgaaactcc gctcaggatg gatggtgagg
     1081 caagggctgg caggcagcgc ccaggccatg gatcgtcagt atcttcttgc gattgaggta
     1141 aggccggaat tcccgtacct ggcaatcgag gcccaaattc tcctgcgccg ggccatgctc
     1201 atacaagacg aaagctggcg tatgtgctgc ctgcaatcgc tttaacgcag cgacatccag
     1261 tatagggatg tcctggcctg cgtagaagac cagtctttca tccagatggc cgaaaccata
     1321 gaacggcacg ttgccctgcg tagccttcca tgcctggaac tgctgcagcg cggaataccg
     1381 gtaatggaac acctgccgtt cagcgaaagc ataataggcg ctgtagcctg ccagcatcaa
     1441 cagactcgcc gtgagcaacg tcagccgctg ccagcgcttg gcggcagcgc atagctgggc
     1501 cagctgctcg cccagcagga tggcgatcca gggatacaag ggcagcaggt acttggcatg
     1561 cttgtcgctg aaagcggtat agagcaggaa tggcaccagc acgaccagcg cgatgcgctc
     1621 ggccattcca tgctgctcgc gccagcggcg ccatctggtc aatggccagg ccaggatcag
     1681 cagcccggcc gggaagaaat cggccagcag ccatagcagg taactgtaga ccggctcccc
     1741 cgtgtggccg tacatcttgt tcatcatgtc atggtggatg gtcgacagcc agatctccgg
     1801 gcccaggcga tagctcaccg ccacgaacca ggcgctgccc acgaccagga acaccagcca
     1861 gccccaagga tcgcgcaaag ccagccatag acgcggatgc ggtcggcata tcgccaatac
     1921 caataacggc aaagtcacga agatcagcgc caacgggccc ttggtcagca ccgcgaggcc
     1981 gagcaaggca taactcagcc atagccaggt gcgcctaccc tcttcgtaga cgaaatggag
     2041 cgccgccaac agtgaagcga agcacagcgt ggagagcaac atctcgatct cggcgcggcg
     2101 ggcaaacaag gcgaagccag cgttggccag tagaagctgc aaggtgaaca gcgcggccag
     2161 gatgccgaaa tgcctgcgcg cgaaccgaaa cgccaggacc gcggccagca aggcggcgaa
     2221 tgccgaaggc aagcgcatcg cccattcact gacgctgccg accagatggg ccgcgattgc
     2281 cgccagccag tagaacaaag gtggcttcgc caggtagagc gcgccatcga gcctgggcaa
     2341 tagccaatcg ccgctttgga acatggcggc cgtgggcagc gcgcgccggg cttcattgta
     2401 ggaaagcagg gggatatcgc ccaggcccca gaacaatagc agaacgacca gcagccaggt
     2461 cgcgggcacg agataccggg gcactacctg cggcgtggtc attgcggcgc actctccagg
     2521 cagtcggcaa gtccggcgac acagcttcga tcccagtggt aggacgcgcg ggtcttggcc
     2581 tggctatcga tgaagctgaa attgaggcta ctggcgtcca gcgccacccg caaaaatcca
     2641 tgttctgcct tgccgaagtg catgtccgac cctggcgatt taagatcgta gagcttggcg
     2701 ccgccggcac cagtgacgat ctgcaacggc tccccttccc tcacgatcag ctgctggttg
     2761 tggtcatggg ccgcgatgta aagatcgacc ccggattgct gcatggcgga gagcaaggca
     2821 ttgtccacgg tcgcctgctc atcctggtga tgtttgccgt aggttctgag cggatgatgg
     2881 ccaacggcga tcttccagat cgggttgcct ccggcggcga actgcttgcg aaggtactcg
     2941 gcctgccctc gcgggtcctg attggtatcc agaaatacaa tgcgcagcaa gggacgctga
     3001 t
//
LOCUS       DATMTW010000170         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_518159, whole genome shotgun sequence.
ACCESSION   DATMTW010000170 DATMTW010000000
VERSION     DATMTW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518159"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..597)
                     /locus_tag="VN084_03150"
     CDS             complement(142..597)
                     /locus_tag="VN084_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HWT28485.1"
                     /translation="MTNEDIINNLNDLIETCKDGEEGFRTCAADIRNVELKQFFIARA
                     VDCAASAEELQQIVIEYDGDPETKSTIAGAMHRRWVDFKAAIMGKDEEAVLDECERGE
                     DIALNHYEEALEEDWPLNVRSVIERQYRGLRENHDQVKYLRDQAAAMHD"
     gene            956..1141
                     /locus_tag="VN084_03155"
     CDS             956..1141
                     /locus_tag="VN084_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28486.1"
                     /translation="MSLFNIEVWPRGKQANVPEWRCELDAENIEQAYMIGGKRFHSER
                     PDLDPEAFIIKASGYLE"
     gene            1138..1428
                     /locus_tag="VN084_03160"
     CDS             1138..1428
                     /locus_tag="VN084_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28487.1"
                     /translation="MNNLEKPLLQLDGLDIPDLYLADVARVYEHADRRGYRYLTIFMI
                     DGVQLAVPATEHNISLILHGGHHIFEYNLANSLLVQEETPASQAFHQQVNAW"
     gene            1425..1949
                     /locus_tag="VN084_03165"
     CDS             1425..1949
                     /locus_tag="VN084_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWT28488.1"
                     /translation="MTAVAQMPWPAIDWTTMFSLSINPLEIFLRGSMMFWFLFAIFRF
                     ILRRGIGAVGIGDFLFVAIVADASQNAMSSDYRSIGDGLILVATLAFWNYTLDYLSFR
                     FVRLRRWIEPGSLVLVKHGKLQRRNLQRQFITTEEIHAKLREDGIASLKHVIEMRLET
                     NGEISVVREPSSQG"
     gene            complement(2071..>2855)
                     /locus_tag="VN084_03170"
     CDS             complement(2071..>2855)
                     /locus_tag="VN084_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWT28489.1"
                     /translation="PEYVRPGYKSGERLQGKVALITGGDSGIGRAVAIAFALEGADVL
                     VQYLNEQEDASETQSLVEEAGASCLLIAGDLADPTFCTHLVERAVEHFGRLDILVNNA
                     AEQHPRDDIREIRTQDLESTFRVNLFSMFHLVQAALPHMGRGARIINTASVNAYRGSP
                     RLVDYSATKGAIVAMTRSLSKQLIDQGIHVNAVAPGPVWTPLIVSTFSAEDVRTFGSD
                     VPMGRAGQPSEIAPCYVFLASEDASYMTGQVMHPNGGVVVNG"
BASE COUNT          601 a          853 c          804 g          597 t
ORIGIN      
        1 ggcgaagcga gcgccgcaaa aaagcccgtc gagcattaac tcgtcgagcc gggtcatcca
       61 ccgccaccat ttctgaatat gaaaaaggca gcgtctacac gctgccttgg gccgagctta
      121 ggaatcagtc atcgtcagtg atcagtcatg catggcagcg gcctgatcgc gcaggtactt
      181 cacctgatca tggttctcac gcaagccccg gtattgacgc tcgatgaccg agcgtacatt
      241 gagcggccag tcttcctcca gtgcttcttc atagtgattg agcgcgatat cttcaccgcg
      301 ctcgcattcg tccagcacgg cttcttcatc cttgcccatg atggccgcct tgaaatcgac
      361 ccagcggcga tgcatggcgc cggcgatggt ggatttggtt tcggggtcgc cgtcatattc
      421 gatgacgatc tgctgcaact cttctgcgct ggctgcgcaa tccacagcgc gggcaatgaa
      481 gaattgcttc agctcgacat tgcggatatc ggcggcacag gtgcgaaaac cttcttcgcc
      541 atccttgcag gtttcgatca ggtcattcag gttgttgatg atgtcttcat tggtcatgtc
      601 gatactccta atttgtatgt tgcgtgttga gggttagcgc aaccgtcggc gacgcatcgc
      661 cctgcaggcg gtcgccgccg ggaatcaagc atcacctgtg tccgggtgat gcatgagcta
      721 gcgtgccctt gcatgttagg cagcgaggca cggcagggtt atcgccccgc gtccgattcg
      781 actgtaggaa gaattgccgg tagtcaggcc tgtaaattcc gcctacagga aatcgggtga
      841 cacactgata aggctatgtt ccgtcggtca ctatgatgtg ttcgccgcta tttgcggcaa
      901 cgtgctgcaa gcgcagcctg atcgcacaga atggaatgga aaggcaggga cgaagatgtc
      961 gctcttcaat atcgaggttt ggcccagggg caagcaggcc aacgtgccgg aatggcgatg
     1021 cgaactggat gcggagaata tcgaacaggc ttacatgatc ggcggcaagc gtttccatag
     1081 cgaacgtccc gacctcgatc cggaggcatt catcatcaag gcaagtggtt acctggaatg
     1141 aacaatctgg agaaacccct gctgcaactc gacggactcg atatccccga cctctatctg
     1201 gcggatgtgg ccagggtcta cgaacacgcg gacagacgag gttaccgcta tctcaccatc
     1261 ttcatgatcg acggcgtaca actcgcagtc ccggccacag aacacaacat cagcctgatc
     1321 ctgcatggcg gccatcatat cttcgaatac aacctggcca acagcttgct ggtgcaggaa
     1381 gaaacaccgg ccagccaggc ctttcaccag caggtcaacg cctggtgaca gcggtggcgc
     1441 agatgccctg gcctgcgatc gattggacga cgatgttctc gctgagcatc aacccgttgg
     1501 agatcttcct gcgtggatcg atgatgttct ggttcctgtt cgccatcttc cgcttcatcc
     1561 tgcgccgtgg catcggcgcg gtcggcatcg gcgatttcct gttcgtggca atcgtcgccg
     1621 acgcttcgca aaacgccatg agcagcgatt accgttccat cggcgacggc ctcatcctgg
     1681 tcgccacact ggccttctgg aactacacgc tcgattactt gagctttcgc ttcgtacgcc
     1741 tgcgccgctg gatcgagccc ggctcgctgg tgctggtcaa gcatggcaaa ttgcagcgcc
     1801 gtaaccttca gcggcaattc atcacgacgg aagagatcca cgccaagctg cgtgaggatg
     1861 gcatcgcttc actaaagcat gtgatcgaga tgcgactcga gaccaatggc gagatcagcg
     1921 tggtgcgcga gccatccagc cagggatagc gatctcaccc cagatgcatt cagacaggcg
     1981 cagcccgacc tgcgctttag ttagttggct ggctcatgca aggaccttgt gcccctggat
     2041 cttgcgctcc cccggctatc cgatcatgac tcagccatta actaccacac cgccattcgg
     2101 gtgcatgacc tgtccggtca tataagaggc atcttcagaa gccagaaaca catagcacgg
     2161 cgcgatctcg ctgggctgtc ccgcccgacc catgggcaca tcgctaccga aggtacgcac
     2221 atcctcggcc gagaacgtag acacgatcaa gggcgtccag accgggccgg gcgccacggc
     2281 attcacatgg atgccctgat cgatgagctg cttggagagc gaacgcgtca tggcgacgat
     2341 cgcgcccttg gtggcggaat aatcgaccaa ccgcggactg cctcggtaag cgttcaccga
     2401 ggcagtgttg atgatgcgtg cgccacgccc catgtgcggc aaagccgcct gcacgagatg
     2461 gaacatcgag aacagattga cccggaacgt gctttcaaga tcctgcgtgc gaatctcacg
     2521 gatatcgtct cgcgggtgct gttccgcagc gttgttgacc aggatatcca gcctgccgaa
     2581 atgctctacg gcgcgttcaa ccaagtgcgt gcagaaggtc ggatcggcca ggtcaccggc
     2641 gatgagcagg cacgaggcgc cagcctcttc caccagggac tgcgtctcgc tggcatcttc
     2701 ctgttcatta aggtattgca ccaacacatc cgcgccctcg agcgcgaaag cgatcgcgac
     2761 agcgcggccg ataccgctgt caccgccagt gatgagagcg accttgcctt gcaacctctc
     2821 cccggacttg taacccggcc ttacatactc cggtt
//
LOCUS       DATMTW010000171         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_148107, whole genome shotgun sequence.
ACCESSION   DATMTW010000171 DATMTW010000000
VERSION     DATMTW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148107"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VN084_03175"
     CDS             <1..273
                     /locus_tag="VN084_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28490.1"
                     /translation="PRTGPRRQIQETWFDYASHTGTAARQRVKLVAEGLAERLKHSAD
                     KAQRKGRQKVKRIGLHAALAASHKLVDLASRIERRLNRLNDLNERQ"
     gene            283..1962
                     /locus_tag="VN084_03180"
     CDS             283..1962
                     /locus_tag="VN084_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWT28491.1"
                     /translation="MIRETFSVMRDFGRLRAIAGILMKYGWGDVAKRLGKGSLIGRAG
                     NAINAGATPEILAQPSEVRARMAMEELGPTFVKLGQVLATRPDIFPPNWISEFAKLQD
                     HVPPVPFAELLPGLEAVLGKSPFEVFRDLQAEPVAAASIAQVHMASLQDGTPVVLKIR
                     RPNIEENIEADLRLLSHLGRLVESEFPEAKRFQPGKIIAQFSKSLRRELDLAMEGRNT
                     DRFARNFAEDETVEFARIHWDYTSESLLVMDHIDGIPGNDLQQAREAGLDLQLLGERG
                     ANAVLKMVLIDGFFHADPHPGNVFYLPGNRLAIIDCGMVGRITLDRRDEIADLLAALV
                     SRDIETLRDILVIWAGDVPIDEDKLSADVDEFICNYDNAPLKHVRFSALLNDLTTIMR
                     ENHLSVPPDLTMLFKALITLEGLGRQLDPDFQIVSHLTPFVKKIIIDRYMPAALFKRG
                     KRGAANVFDALTGLPGDVSQVLREAGRGRLRLNLELKRLDHFGHQLDHSTNRLTLGLI
                     TAALIVGSSIVMTVKGGPTLFGLPAFGFMGFFLAFVIGIMLMISVWRSGRD"
     gene            2124..3545
                     /locus_tag="VN084_03185"
     CDS             2124..3545
                     /locus_tag="VN084_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWT28492.1"
                     /translation="MDTSAPLPFDEMRSADGTVRDIYADYADWIRNIPHHQLESKRQE
                     AELLFRRVGITFNVYGETAGKERLIPFDIIPRLLAASEWKRLSAGAIQRVKALNMFLH
                     DIYHGQEIIKAGIVPASIVANTQYRPEMFGVDVPGQVYAHIAGIDIVRTAEDQFYVLE
                     DNLRTPSGVSYMLEDRKMMMRLFPELFRRYSVAPVEHYPQVLMNNLRAVAQPGVQDPV
                     VVLLTPGAYNSAYFEHAFIAQQMGVELVEGQDLFVRNNAVYMRTTEGPKRVDVIYRRI
                     DDDYIDPLVFKPDSMLGVPGLLSVYRNGGVTLANAVGTGVADDKATYIHVPEMIRFYL
                     GEEPILNNVPTYELSKADDLKYVLANLKELVVKEVQGSGGYGMLVGPAATTAEIEEFR
                     LRILSAPSNYIAQPTLALSTCPTLVEQGIAPRHVDLRPFVLSGRTVTLVPGALCRVAL
                     KEGSLVVNSSQGGGTKDTWVLNA"
     gene            3729..>3958
                     /locus_tag="VN084_03190"
     CDS             3729..>3958
                     /locus_tag="VN084_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWT28493.1"
                     /translation="MLSRTADHLYWMARYIERAENMARVLDVTYNMSLVPNAAESEAA
                     LWGPALRIAGDVIAYEAEYGPITAAGVIRYLA"
BASE COUNT          759 a         1229 c         1194 g          776 t
ORIGIN      
        1 ccaaggactg gcccgcgccg acaaatccag gaaacatggt tcgattatgc gagccatacc
       61 ggcacggcgg cgcgccagcg cgtcaagctg gttgccgaag ggctggccga acggctcaag
      121 cattccgccg acaaggccca gcgaaagggc cgccagaaag tgaaacgcat cggactccac
      181 gccgcgctgg cagccagcca taagctggta gacctcgcca gccggatcga gcgccgcctc
      241 aaccgactca atgacctcaa tgaacgccaa taagggcaag caatgatccg tgaaaccttc
      301 tccgtgatgc gagatttcgg ccgcctgcgc gccatcgctg gcatcctcat gaaatacggc
      361 tggggcgatg tcgccaagcg tctcggcaag ggtagcctca tcggacgtgc gggcaacgcg
      421 atcaatgccg gcgcgacgcc cgagatcctt gcgcagccct ccgaggtgcg cgcccgcatg
      481 gcgatggaag agctgggccc gaccttcgtc aagctgggcc aggtgctggc gacccgcccc
      541 gatatctttc cgcccaactg gatcagcgaa ttcgccaagt tgcaggacca cgtcccaccc
      601 gtgccgtttg ccgagttatt gccgggcctg gaagcggtgc tgggcaaatc gccgttcgaa
      661 gtgttccgcg acctgcaggc cgagcccgtc gcggccgcct cgatcgcgca agtgcacatg
      721 gccagcctgc aggatgggac acccgtggtg ctgaagatac gccggcccaa catcgaagag
      781 aacatcgagg ccgacctgcg tctgctgtcg catctcgggc gactggtcga gtcggaattc
      841 cccgaagcca agcgtttcca gcccggcaag atcatcgcgc aattctcaaa atcgctgcgc
      901 cgcgagcttg atctcgcgat ggaaggccgc aataccgatc gcttcgcacg caatttcgcc
      961 gaggacgaga ccgtggagtt cgcccgtatc cactgggatt acaccagcga atcgctgctt
     1021 gtcatggacc acatcgacgg catccccggc aacgatctgc agcaggcgcg cgaggctggc
     1081 ctcgatctgc aactgctcgg cgaacgtggc gccaatgcgg tgctgaagat ggtgctgatc
     1141 gacggtttct tccacgcaga tccgcacccg ggaaacgtgt tctacctgcc gggcaatcgg
     1201 ctggcgatca tcgattgcgg catggtcggg cgcatcacgc tcgaccggcg cgatgagatc
     1261 gccgacttgc tggcagcgct ggtctcgcgc gatatcgaga ctttgcgcga tatcctcgtc
     1321 atctgggcag gtgacgtgcc gatcgatgaa gacaagcttt ccgccgatgt cgacgaattc
     1381 atctgcaact acgacaatgc gccgctcaag catgtgcggt tcagtgcact gctcaatgat
     1441 ctcaccacca tcatgcgcga gaaccatctg tcggtgccgc ctgacctgac catgctgttc
     1501 aaggcgctga tcacgctcga aggactgggg cgccagctcg atccggattt ccagatcgtc
     1561 agccacctga cgccgttcgt gaagaaaatc atcatcgacc gctacatgcc agcggcattg
     1621 ttcaagcgcg gcaaacgtgg cgcggccaat gtctttgatg ccctgaccgg tttgccgggc
     1681 gatgtctccc aggtcttgcg tgaggcaggg cgcggtcggt tgcgcctcaa cctcgagctg
     1741 aaacgactcg atcatttcgg ccaccagctc gatcacagca ccaaccggct gaccctgggg
     1801 ctcatcacgg cggcgctgat cgtcggctct tccatcgtca tgaccgtcaa gggtgggccg
     1861 acactgttcg gcttgcctgc cttcggtttc atggggtttt tcctggcttt cgtcatcggc
     1921 atcatgctga tgatctcggt ctggcgatcc gggcgcgatt agcgcctgtc tacccgccaa
     1981 gagccggcgg ccccaagtcg gcgcacccgt tccagccgcc gccccatgcg ggtgcgttgt
     2041 gctgtccagc tggcctggat attttataat ccgttgatat aaaacgatta aatttctggc
     2101 atgtagtttg cctgaggtaa tgcatggata cctcagcacc ccttccgttt gacgagatgc
     2161 gctcggccga tggcaccgtt cgcgacatct acgcggacta tgccgactgg atccgcaaca
     2221 tcccgcacca tcagcttgaa agcaagcggc aggaagccga gttgctgttc cgacgcgtcg
     2281 gcatcacgtt caacgtctat ggcgagacgg ccggcaagga acgtctgatc ccgttcgata
     2341 tcatcccgcg cctattggcc gccagcgaat ggaaacggct gtcggcgggc gccatccagc
     2401 gcgtgaaagc gctcaacatg ttcctgcacg acatctacca cgggcaggag atcatcaagg
     2461 ccggcatcgt accggccagc atcgtcgcca atacgcagta ccggcccgag atgttcggcg
     2521 tcgacgtgcc ggggcaggtc tacgcgcaca tcgccggtat cgatatcgtg cgcaccgccg
     2581 aagaccagtt ctatgtgctg gaggacaacc tgcgcacgcc gtctggcgtg agttacatgc
     2641 tcgaggaccg caagatgatg atgcggctgt tcccggagtt gttccgccgt tattcggtgg
     2701 cgccggtcga gcattatccg caggtgctga tgaataacct gcgtgcggtc gcccagccag
     2761 gggttcagga tccggtcgtg gtgctgctga cgcccggtgc ctacaacagc gcctatttcg
     2821 agcatgcctt catcgcccag cagatgggcg tggaactggt ggaagggcag gacttgttcg
     2881 tgcgcaataa cgcggtctac atgcgtacga cggaaggccc caagcgcgtc gatgtcatct
     2941 atcgccgcat cgacgacgat tacatcgatc cgctggtgtt caagcctgat tccatgctgg
     3001 gcgtgcccgg cttgctgtcg gtctatcgca acggtggcgt gaccttggcc aacgctgtcg
     3061 gcactggggt ggcggatgac aaggccacgt atatccatgt gcccgagatg atccgtttct
     3121 acctcggcga ggaacccatc ctgaacaacg tgccgacgta tgagcttagc aaggccgatg
     3181 acctcaagta tgtgctcgcc aacctcaagg agctggtcgt gaaggaggtg cagggctccg
     3241 gcggttacgg catgctggtg gggccggccg caacgacggc cgagatcgag gaattccggt
     3301 tacgcatcct ttctgcgccc tcgaattaca tcgcccagcc gaccctggcg ctttcgactt
     3361 gtccgacatt ggtcgagcag ggcatcgccc cgcgccatgt cgatctgcgg ccattcgtcc
     3421 tttccggcag gaccgtcacg ctggtgccgg gggcactctg ccgcgtcgct ttgaaggaag
     3481 gctcgcttgt ggtcaattcc tcgcagggtg ggggcaccaa agacacctgg gtcctcaatg
     3541 catgactgag cggctgcatg aacgaatcgc tgcgttgcgc ggtgctcgca tactgcgcgc
     3601 cgtgggcctt gtgcttcatc tcatccgctc gctcctctgt tcagtggggt tggcagttaa
     3661 caaagaaggc tcagtgaatt cacgcaattc cgcaagacgg ctgcgcaaaa agtcagtctg
     3721 gagttcagat gttaagtaga accgccgatc acttgtactg gatggcccgt tacatcgagc
     3781 gtgccgagaa catggcgcgc gtgctcgatg tcacttacaa catgtcgctg gtgcctaacg
     3841 cagccgaaag cgaggctgcc ttgtggggtc cggccctgcg catcgcggga gacgtcatcg
     3901 cctacgaggc ggaatatggc ccgatcactg ccgccggcgt gatccgctac ctggcgat
//
LOCUS       DATMTW010000172         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_282720, whole genome shotgun sequence.
ACCESSION   DATMTW010000172 DATMTW010000000
VERSION     DATMTW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282720"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN084_03195"
     CDS             <1..275
                     /locus_tag="VN084_03195"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWT28494.1"
                     /translation="IRHGVRKVNIDTDLRMAFVGAVRQHLMLAENNKDLDFRRFLLAA
                     TKGMQAICRQRFEAFGSAGRASAIKPIGCDKMAERYRKGELAAVVT"
     gene            332..1120
                     /gene="tpiA"
                     /locus_tag="VN084_03200"
     CDS             332..1120
                     /gene="tpiA"
                     /locus_tag="VN084_03200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWT28495.1"
                     /translation="MRRKLVVANRKMHGSVPANQAFLRSLLVETRGLHDADIVVCVPH
                     PYLFQVQAMLTGSHIAWGDQHVSRFTLGAHTGSVSASMLLEFGCQYTIIGHSERRAWG
                     YETDLTSAQRFETAIRAGLRPIYCVGDTREEYLAGETDKVTFRQLSAIIDHVGVAGLA
                     QGVLAYEPVWAIGTGQAASPAHVQMILGLMRKHIASLDPTLAAEIRILYGGSVKAGNA
                     AALFSMPDVDGALVGSASLVTDEFIAICQAANEAGKSLRADIAS"
     gene            1230..2126
                     /locus_tag="VN084_03205"
     CDS             1230..2126
                     /locus_tag="VN084_03205"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWT28496.1"
                     /translation="MTPALMRSSIHSLKPLHQGKVRDIYDVDAQTMLLIATDRLSAFD
                     VVLPTPIPEKGAILTQIANFWFNKLGHVVPNHLTGIDPDSLVTDPLEKAQLGRRALVV
                     KKLKPLPIEAIVRGYIAGSGWKEYKASGTVCGIALPAGLQEAAKLPEPIFTPSSKAAV
                     GDHDENISLARAAELLGQDLADKVAQVSLQLYKEAAAYALTRGIIIADTKFEFGLDAQ
                     GTLHLIDEALTPDSSRFWPADQYVVGKSPPSYDKQYVRDWLESTGWDKTPPGPELPAE
                     VARKTGEKYREAYEKLTGKAYA"
BASE COUNT          441 a          700 c          716 g          406 t
ORIGIN      
        1 gcatccgcca cggcgtgcgc aaggtcaata tcgacaccga cctgcgcatg gccttcgtcg
       61 gcgccgtgcg ccagcatctg atgctggcgg agaacaataa ggatctggat ttccggcgct
      121 ttttgctggc ggcgaccaaa ggcatgcaag ctatctgccg ccagcgtttc gaggcattcg
      181 gctctgccgg gcgcgcttct gcaatcaaac ccatcggctg cgacaagatg gcagagcgtt
      241 accgcaaggg cgagctcgcc gccgtggtga cctgatccgc agatgtgcac ggggaggggc
      301 atatgaagaa cggacatgcg gggcgctggg catgaggcgc aagctggtgg ttgccaaccg
      361 caagatgcac ggtagcgttc ccgccaacca ggctttcctg cggagtttgc tggtcgagac
      421 ccgcggtctc catgacgccg atatcgtcgt ttgcgtcccg catccgtatc tctttcaggt
      481 gcaggcgatg ctcaccggca gccatatcgc gtggggcgac cagcatgtca gccgcttcac
      541 gctgggcgcc cataccggct cggtctcggc cagcatgctg ctggagttcg gctgccagta
      601 caccatcatc gggcattccg aacggcgtgc ctgggggtat gagacggacc tgacctcggc
      661 acagcggttc gagacggcga tccgcgccgg cctcaggcca atctactgtg tcggtgatac
      721 acgcgaggaa tacctcgctg gcgaaaccga caaggtgacc ttccgccagt tgagcgcgat
      781 catcgatcac gtcggggtcg ctggattggc acaaggcgta ttggcctatg agccggtctg
      841 ggcgatcggc accgggcagg cggcaagccc ggcccatgtg cagatgatcc tggggctgat
      901 gcgcaagcat atcgccagcc tggatccaac gctggcggcc gagatccgta tcctttacgg
      961 cggtagcgtc aaggctggca atgcggcggc cctgttctcc atgccggatg tggacggcgc
     1021 gctggtgggc tcggcttcgt tggtcaccga cgagttcatc gccatctgcc aggcggcgaa
     1081 cgaggccggc aaaagcttgc gggccgatat cgcatcctga gtgcctgaga atgcaggaat
     1141 cggcacgcgc gcagttataa taccgtctcg catcatgtcc gaggccaacc ggccaacgga
     1201 ccatcaccga aggatcgagg gagaccgttt tgacgccagc actcatgcgc agcagcatcc
     1261 acagcctgaa acccctgcat cagggcaagg tgcgcgatat ctatgacgtc gatgcgcaga
     1321 ccatgctgct gatcgcgacc gatcgcctgt cggcgttcga tgtggtgttg ccgacgccga
     1381 tcccggaaaa gggcgcgatc cttactcaga tcgccaattt ctggttcaac aagctcggac
     1441 atgtcgtccc taaccacctg accggtatcg atcccgacag cctagtcact gacccattgg
     1501 agaaggccca gctcggtcga cgtgcgctgg tggtgaagaa gctcaagccc ctgccgatcg
     1561 aagccatcgt gcgcggttac atcgccggct ctggctggaa ggaatacaag gccagtggca
     1621 cggtctgcgg catcgccctg cctgccggcc tgcaggaggc ggcgaaattg cccgagccca
     1681 tcttcacgcc atcaagcaag gcggcggtgg gcgaccatga cgagaacatc agccttgccc
     1741 gcgcggcgga attgctgggc caggatctgg ccgacaaggt tgcccaggtt agcctgcagc
     1801 tctacaagga agccgccgcg tatgcgctga ctcgcggcat catcatcgcc gataccaaat
     1861 tcgaattcgg cctcgatgcg caaggcacac tgcatctcat cgacgaggcg ctgacgccgg
     1921 attcgtcgcg cttctggccg gctgaccagt acgtggtggg caagagcccg cccagctacg
     1981 ataagcagta cgtgcgcgac tggctggaat cgaccggctg ggacaagacg ccgccgggac
     2041 cggagttgcc agccgaggtg gcacgcaaga ccggcgaaaa gtaccgcgag gcgtacgaga
     2101 aactcaccgg gaaagcctac gcctgaaact gcgtgagctc acccgccagg tcaagacaga
     2161 gcttgcggtt tacaagttgg tgttgcgtca tgcgcgtacc ccgcgcgtgg ccaaatggtt
     2221 gctctggctc gcgatcgctt acctactgct gccattcgac ctg
//
LOCUS       DATMTW010000173         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_585440, whole genome shotgun sequence.
ACCESSION   DATMTW010000173 DATMTW010000000
VERSION     DATMTW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585440"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..825)
                     /locus_tag="VN084_03210"
     CDS             complement(55..825)
                     /locus_tag="VN084_03210"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWT28497.1"
                     /translation="MLPKADRPTKGKTWPKPAGAKRLREYHIYRYDPDQSANPRIDTY
                     FVDLDDCGPMILDGLLWIKNKVDPTLTLRRSCREGICGSCSMNINGLNTLACTKGMDD
                     STGPIKIYPLPHMPVVKDLVPDLTTFYAQHRAIEPWLKTTSPTPAKEWTQSVPQRAKL
                     DGLYECILCACCSTSCPSYWWNGEKYLGPAALLQAYRWLIDSRDETTQERLDDLEDPF
                     KLYRCYTIMNCAKVCPKGLNPAKAIAEIKKMMVERQAA"
     gene            complement(877..1161)
                     /locus_tag="VN084_03215"
     CDS             complement(877..1161)
                     /locus_tag="VN084_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28498.1"
                     /translation="MRIFAIAAAFGLLTLPALGAPTAEQKAEFYAVCMGIAQDETLCS
                     CKADAAMTLIDADFMAMVISAMQGGSVPQDEYSAYNAYVAKSNQICKPNY"
     gene            complement(1158..1589)
                     /locus_tag="VN084_03220"
     CDS             complement(1158..1589)
                     /locus_tag="VN084_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28499.1"
                     /translation="MSNAAGYSGTPLAQKLGLKDGQRALFIDLPASLAELAASRQFVE
                     SSRITVAQLGNAGPGYHFIHLFTAARSVLESLAQPLMNLIARDGMIWVSWPKKAAKVA
                     TDITEDVIRDVVLPIGLVDVKVCAVDETWSGLKLVIRKELR"
BASE COUNT          297 a          480 c          556 g          322 t
ORIGIN      
        1 cctcaagggg gagggtgggc ccggtggttg ggaggaattt ggtaccccga aaaactaagc
       61 cgcctggcgt tccaccatca tcttcttgat ttcggcgatg gccttggccg ggttcagccc
      121 cttggggcag accttggcgc aattcatgat ggtgtagcag cgatagagct tgaacgggtc
      181 ttcgaggtcg tcgaggcgtt cctgggtggt ctcgtcgcgc gaatcgatca gccagcgata
      241 ggcctgcagc aaagccgccg ggccgagata cttctcgccg ttccaccaat agctggggca
      301 cgaggtggag cagcaggcgc agaggatgca ctcatagagc ccgtccagct tggcgcgctg
      361 gggaaccgac tgcgtccatt ccttggccgg ggtgggcgaa gtggttttga gccagggctc
      421 gatggcgcgg tgctgggcat agaaggtggt gagatcgggc accaggtcct tgaccacggg
      481 catgtgcggc agcgggtaga tcttgatcgg acccgtcgag tcatccatgc ccttggtgca
      541 ggccagggta ttgagcccgt tgatattcat cgagcacgag ccgcaaatgc cctcgcggca
      601 ggagcggcgc agcgtcaggg tagggtcgac cttgttcttg atccagagca ggccatccag
      661 gatcatggga ccgcaatcgt ccagatcgac gaaataggtg tcgatgcgcg gattggcgct
      721 ctggtcgggg tcatagcgat agatgtgata ctcgcgcagc cgcttggcgc cggcgggctt
      781 gggccaggtc ttgcccttgg tcggccgatc ggccttgggg agcatcagtt cgaccattgg
      841 tcggttcctt cttaattggg ccagcagccc aaggcattag tagttcggct tgcagatctg
      901 gttggatttg gcgacgtagg cattgtaggc cgagtactcg tcctgcggca ccgagccgcc
      961 ctgcatggcc gagatcacca tggccatgaa atccgcgtcg atcagcgtca tcgccgcgtc
     1021 ggccttgcac gagcacagcg tctcgtcctg ggcgataccc atgcagaccg cgtagaactc
     1081 ggccttctgc tcggccgtgg gggccccgag cgcgggaagc gtcagcaggc cgaaagcagc
     1141 ggcaatggcg aaaattctca tctcagctcc ttgcggatga ccagcttgag gccggaccag
     1201 gtttcatcga cggcgcaaac cttcacgtcc accagtccga tgggcagcac cacgtcgcga
     1261 atgacatcct cggtaatatc ggtggcgacc ttcgccgctt tcttgggcca gctgacccag
     1321 atcatcccgt cacgcgcgat gaggttcatc agcggctggg cgagggattc gagcacggac
     1381 cgggcggcgg tgaagaggtg gatgaagtga tagcccggcc cagcattgcc aagctgggcg
     1441 acggtgatgc ggctcgactc gacgaactgc cgcgatgccg cgagttcggc cagcgacgcg
     1501 ggcaggtcga tgaacagcgc ccgctgccca tctttcagcc ccagcttctg cgccaggggc
     1561 gtgcccgaat atccggccgc gttgctcatc aatagacgcg cgccttgggc gcgatcttct
     1621 tgaggtcgat gccaccctga tcgtaagggg tcagc
//
LOCUS       DATMTW010000174         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_585527, whole genome shotgun sequence.
ACCESSION   DATMTW010000174 DATMTW010000000
VERSION     DATMTW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585527"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VN084_03225"
     CDS             <1..398
                     /locus_tag="VN084_03225"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWT28500.1"
                     /translation="LALAIMQAESNFNPAAVSPKNAQGLMQLIPATAERFNVKNAFDA
                     TQNIKGGIAYIRWLLSYFRGDVRLAVAAYNAGEGAVNRYKGIPPYQETRQYVRKVMAL
                     YGQASHPYDDTLASPSPVVSKDIDASAGE"
     gene            504..1040
                     /locus_tag="VN084_03230"
     CDS             504..1040
                     /locus_tag="VN084_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28501.1"
                     /translation="MMKRWINLGSMLLTSALLLLSGCATTGVQEPQIKRISQEELERL
                     MPKPTPNLTLEEIVKLSKAGTSPEDIIEKIKASNSTYALTPSQVVDLNKQGVSPKVLD
                     YIQQAREQVMRDAYADEINKREKAKVEETQRLRREYQMRSYPYYDPWWGYSYSPYYGY
                     RPFYRPGFGTRFHYGWGW"
     gene            complement(1044..>2668)
                     /locus_tag="VN084_03235"
     CDS             complement(1044..>2668)
                     /locus_tag="VN084_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWT28502.1"
                     /translation="MHQHQIPRKIMLLRYFHRAAEPARFAVLRDVPLFSGLSAHELET
                     LDGLLHQRAYVKDEVIFDQDEEGQAIYFVLSGKVRISRQDQSDAVAVIEPGQFFGERA
                     LLEHLPRIAKAQVEEDCILAVLFRDDFLGLLHSYPQIAEKISQHCSLRDTHRILQLVP
                     SKPPRLLGELREIPGPLTWVGIIASTCLTLFMFKKILWLVVPFLFALILYYLLAPIAK
                     RMVLAGLSSGMAALTLSGGMLLVTMLIVFLFYPFAMANGEYWQEAILRYLNGATTMLD
                     QLLQSIQVHFSFLSKTDLSHKMWVEVKDFTEHFSDRYLGDLLLGIAAWLPSLLLTPII
                     TFFLLKDGAYLRKMLGSAVPNAFFEKTLHLMHAVDRTARMYFVGLMKMTVIDAVMTTL
                     GLWFLGFDSPLLLGLMVAVLCWIPYLGPLLGLAIVLMVGATDFPGNLPLVYGVLTLFA
                     TMRALDDFVFMPWVVGRSLHLHPLLTVLMFLIGEAIAGVAGLMLVIPILGIIMVLGET
                     VEIILTDTRLQARHRYARRLRWRAATRDLAGF"
BASE COUNT          597 a          818 c          815 g          438 t
ORIGIN      
        1 agttggcgct ggcgatcatg caggccgagt ccaacttcaa cccggccgcc gtctcgccca
       61 agaacgcgca ggggctgatg cagctcatcc ccgccacggc ggagcgtttc aacgtcaaga
      121 acgcgttcga cgccacgcag aatatcaagg gcggtatcgc ctatatccgg tggttgttgt
      181 cgtatttccg cggtgacgtg cggctggcgg tcgccgccta caacgccggc gagggggcgg
      241 tgaaccgcta caagggtatc ccgccgtatc aggagacacg gcaatacgtg cgcaaggtga
      301 tggcgctcta cggccaggct tcgcatcctt acgacgacac gctggcaagc ccgtcgccgg
      361 tggtcagcaa ggatatcgac gcatcggccg gcgagtgagc gcaacccgcg aatatcgcat
      421 gcattcaacc ggcgacccgg ctggcgcgtt ataaagcgcg cgttgagcgt gtaagtgcac
      481 ggcaaacgaa taaggacagt cacatgatga aacgatggat caatctcggc agcatgctgc
      541 tgaccagcgc gctattgcta ctctccggtt gcgccacgac cggcgtgcag gagccgcaga
      601 tcaagcgcat ctcgcaggag gagctggaac ggctgatgcc caagccgacg cctaacctga
      661 cgctggaaga gatcgtcaag ctgtccaagg cgggcacgtc accggaagac atcatcgaga
      721 agatcaaggc gtcgaactcg acatatgcgc tgacgccatc gcaggtggtc gacctcaaca
      781 agcagggggt cagccccaag gtgctcgact acatccagca ggcgcgcgag caggtcatgc
      841 gcgacgccta tgccgacgag atcaataagc gcgagaaagc caaggtcgag gagacacagc
      901 ggttaagacg cgaataccag atgcgttcct acccgtatta cgatccgtgg tggggttaca
      961 gctacagccc gtactacggc tatcgcccat tctaccggcc gggcttcggc acccgcttcc
     1021 actacggctg gggctggtag gcgctagaac cctgccagat cacgcgttgc ggcgcgccag
     1081 cgcagccgcc gagcataccg atgcctggcc tgcaggcgcg tatcggtaag gatgatctcc
     1141 accgtctcac ccagcaccat gatgatgccg aggatgggga tcaccagcat caaccccgcc
     1201 actccggcga tcgcctcgcc tatcaggaac atcagcaccg tcagcagcgg atgcaggtgc
     1261 aggctgcggc cgaccaccca gggcatgaag acgaagtcat ccagcgcgcg catcgtggcg
     1321 aacaatgtca gcacgccata gaccagcggc aggttccccg gaaaatcggt agcaccgacc
     1381 atgagcacga tggcgaggcc aagcaacggt ccgaggtagg ggatccagca cagcacggcg
     1441 accatcaggc cgagcagcag cggcgaatcg aagccgagga accacaggcc cagcgtcgtc
     1501 atcacggcat cgatcaccgt catcttcatc agcccgacga aatacatgcg cgcggtgcga
     1561 tccaccgcat gcatgaggtg cagggtcttt tcaaagaacg cgttgggcac cgcgctgccc
     1621 agcatcttgc gcaggtaggc gccgtctttc agcaggaaga aggtgatgat cggcgtcagc
     1681 agcagcgacg gtagccacgc cgcgatgccg agcagcaaat cacccagata gcggtcggag
     1741 aagtgttcgg tgaagtcctt cacctccacc cacatcttgt ggctgaggtc agtcttgctg
     1801 agaaagctga aatgcacctg gatcgattgc agcagttgat cgagcatggt cgtggcgccg
     1861 ttcaggtaac gcaggatcgc ctcctgccag tattcgccgt tggccatggc aaagggatag
     1921 aacaggaaca cgatcagcat cgtcaccaac agcatcccgc cgctgagcgt caacgccgcc
     1981 atgccgctgc tcagcccggc cagcaccatg cgcttggcga tcggcgccag caggtaatac
     2041 aggatcagtg cgaacaggaa aggcaccacc agccacagga tcttcttgaa catgaacaag
     2101 gtgaggcagg tgctggcgat gatgccgacc caggtcagcg gcccgggaat ctcgcgcaac
     2161 tcgcccaaaa gacgcggcgg cttgctcggc accagctgca ggatgcgatg cgtatcgcgc
     2221 aggctgcagt gctgggagat cttctcggcg atctgcgggt aactgtgcaa caggccaagg
     2281 aaatcgtcac ggaacagcac cgccaggatg caatcctcct cgacttgtgc cttggcgata
     2341 cggggcaggt gttccagcag cgcccgctca ccgaagaatt gcccgggctc gatcacagcc
     2401 acggcatcgc tttggtcctg gcgtgagatg cgcaccttgc ccgacagcac gaagtagatc
     2461 gcctgacctt cctcgtcctg atcgaagatg acctcatcct tcacgtacgc gcgctggtgc
     2521 agcaggccgt cgagcgtctc tagctcgtgc gcgctcagtc cagagaacaa gggcacatcg
     2581 cgcaggacgg cgaagcgggc gggttctgca gcacgatgga agtagcgtaa tagcatgatt
     2641 tttcttggaa tctggtgttg atgcatct
//
LOCUS       DATMTW010000175         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_82577, whole genome shotgun sequence.
ACCESSION   DATMTW010000175 DATMTW010000000
VERSION     DATMTW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82577"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN084_03240"
     CDS             <1..219
                     /locus_tag="VN084_03240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168799.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWT28503.1"
                     /translation="TLWMVKRVFYGDIANPHVAALKDINKREALILSVLAIMVIGFGV
                     YPQPITELTHATVNNLLSHLAQSKIPGI"
     gene            223..1671
                     /gene="nuoN"
                     /locus_tag="VN084_03245"
     CDS             223..1671
                     /gene="nuoN"
                     /locus_tag="VN084_03245"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWT28504.1"
                     /translation="MNSIQFDLIAVLPEIFVLSMAMFILLLDLFLKSNTRILLYIFSQ
                     LTLLGAAFITISTHTPSVSYAFTGMFVDDPMADVLKLMIYLGTSMLLAYSRVYIALRG
                     MFRGEFYALVLFAMAGMMVMVSGQNFLTLYMGLELLSLSLYALVALDRDNPRATEAAM
                     KYFVLGALASGMLLYGMSMLYGMTGSLNISQVSAALLNGVPDHSVLILGLVFIVAGLA
                     FKLGAVPFQMWVPDVYQGSPTAVTLLIGSVPKLAAFAFVIRMLAQGMFVLAIDWQGML
                     VIMAVLSIAIGNITAIAQTNLKRMLAYSTISHIGFLLFGLMSASINGYASAMFYVSSY
                     VLMTLAAFGIIMLLSRKGFEAENLDDLKGLNQRSPWYAFLMLIVMFSMAGVPPTLGFY
                     AKFTVLQAALHAGFVWLVVFAVLMAAIGAFYYLRVVKLMYFDEPEDHSPIVAHPELNM
                     VLSVNAVGLLILGLMPQRLMDICAYAILRSLQ"
     gene            1674..1964
                     /locus_tag="VN084_03250"
     CDS             1674..1964
                     /locus_tag="VN084_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HWT28505.1"
                     /translation="MTNWLLLSLAFLAANLPWFSERLFYVIPLKRQPKNIGWCALELI
                     VLYFVMGAVGFYTESATVGQSAPQHWEFYAITASLFLVFAFPGFVYRYFWKQ"
     gene            1975..2541
                     /locus_tag="VN084_03255"
     CDS             1975..2541
                     /locus_tag="VN084_03255"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWT28506.1"
                     /translation="MSQDEDLTETTLSSETVFDGNLLHVLRDNARLPNGHQSMREFIV
                     HPGAVIVIPVLPNGNLLLERQFRYPLKQVFIELPAGKIDPNEDILVTGQRELLEETGY
                     TARRWDYVAKQHPCIGYSNEVIHIYLARDLSAGEHQRDEDEHLQLFDASLPDCLRMVR
                     DGAITDGKTIIALFWAEKVLQDAWPLDT"
     gene            2538..>2857
                     /locus_tag="VN084_03260"
     CDS             2538..>2857
                     /locus_tag="VN084_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWT28507.1"
                     /translation="MTARVLIAGCGDLGLGVVSALAHARFDIHGLRRSAQPFPNGMTA
                     IQADVTRPDSLGALRTLRPDILLYCVAADAQTDDSYRTHYVDGLRNVLATLDPAQLRH
                     VFLVS"
BASE COUNT          509 a          908 c          770 g          670 t
ORIGIN      
        1 acgctttgga tggtgaagcg cgtgttctat ggcgatatcg ccaacccgca tgtcgccgcg
       61 ctcaaagata tcaacaagcg tgaagccctg atcctgtctg tgctggccat catggtcatc
      121 gggtttggcg tctatccgca gccgatcacc gagctgacgc acgccacagt caacaacctg
      181 ctatcacatc ttgcacagag caagatcccg gggatttagc gaatgaatag catccaattc
      241 gacctgatcg ccgtcctgcc cgagatcttc gtgctctcga tggcgatgtt catcctgctg
      301 ctggacctgt tcctcaagtc caatacgcgc atcctgctct atatcttctc gcagctgacc
      361 ctgctcggcg ccgcttttat caccatcagt acgcatacgc cttcggtcag ctatgccttc
      421 accggcatgt tcgtcgacga cccgatggcc gacgtgctga aactcatgat ctatctcggc
      481 acctcgatgc tgctggcgta ttcgcgcgtc tacatcgctt tgcgcggcat gttccgcggc
      541 gagttctacg cactggtgtt gttcgccatg gcgggcatga tggtgatggt ctccggccag
      601 aacttcctca cgctctacat ggggctggaa ctcttgtcgc tgagcctcta tgcgcttgtg
      661 gcactggatc gtgacaaccc gcgtgcgaca gaagccgcga tgaagtattt cgtgctcggc
      721 gcgctggcct ccggcatgtt gttgtacggc atgtccatgc tctacggcat gaccggcagc
      781 ctcaatatct cgcaggtctc cgcggccttg ctcaatggcg taccggatca ctcggtgttg
      841 atccttggcc tggtgttcat cgtcgccggc cttgccttca agctcggtgc cgtgcctttc
      901 cagatgtggg tgccagacgt ctaccagggc tcgccgacgg cggtgaccct gctcatcggc
      961 tcagtaccaa aacttgcggc attcgctttc gttatccgca tgctggcaca gggcatgttc
     1021 gtgctcgcga tcgactggca aggcatgctg gtcatcatgg cagtgctatc catcgccatc
     1081 ggcaacatca ccgccatcgc ccagaccaac ctcaagcgca tgctggcgta ttccaccatc
     1141 tcccacatcg gtttcctgct gttcggcctg atgagcgcta gcatcaacgg ttatgcctcg
     1201 gccatgttct atgtctcgtc ctacgtgctg atgactctgg ccgctttcgg catcatcatg
     1261 ttgctgtcgc gcaagggctt cgaggccgaa aacctggatg acctcaaggg cctgaatcag
     1321 cgcagtccct ggtatgcctt cctcatgctc atcgtcatgt tctccatggc cggcgtacca
     1381 ccgacactgg gcttctatgc caagttcacc gtgttgcagg cagccttgca tgctggtttt
     1441 gtctggctcg tggtctttgc ggtgctgatg gccgccatcg gcgcgttcta ctacctgcgc
     1501 gtggtcaaac tcatgtactt cgacgaaccg gaagaccaca gcccgatcgt cgcccatcct
     1561 gaactcaaca tggtactgag cgtgaatgct gtcggtctgc tgatcctggg cttgatgccg
     1621 cagcggctga tggatatctg cgcctatgcc attttaagaa gcctgcaata accatgacca
     1681 actggctgct gttgtctctg gcattcctcg ccgcgaatct gccgtggttc tctgaacgcc
     1741 tgttctacgt catcccgctc aagcgccagc ccaagaacat cggctggtgc gcgctggaac
     1801 tgatcgtcct gtacttcgtc atgggcgcgg ttgggttcta caccgagagc gcgaccgtgg
     1861 gccaatcggc gccacagcac tgggagttct acgccatcac cgccagtctg ttcctggtct
     1921 tcgctttccc cggcttcgtt tatcgctatt tctggaagca atgattcgcg cggcatgagc
     1981 caggacgagg atctcaccga aaccacactc agttccgaga ccgtcttcga cggcaatctg
     2041 ctgcatgtcc tgcgcgataa cgcgcgcctg cccaacggcc accagtcgat gcgcgaattc
     2101 atcgtgcatc cgggcgcggt gatcgtcatt ccggtgctgc ctaacggtaa cctgctgctc
     2161 gagcgccaat tccgttatcc cctcaagcaa gtcttcatcg aactgcccgc gggcaagatc
     2221 gatcccaatg aggatatcct cgtcactggc cagcgcgagc tgctggaaga gactggctac
     2281 acggcacgac gctgggacta cgtggccaag cagcatccct gcatcggtta ctccaacgaa
     2341 gtcatccata tctaccttgc tcgcgacctc agtgcaggcg agcaccaacg ggatgaggat
     2401 gaacatctgc agctgttcga tgccagcttg ccggattgcc tgcgcatggt gcgtgatggc
     2461 gcgatcaccg acggcaagac catcatcgcg ttgttctggg ccgagaaggt attgcaggac
     2521 gcctggcccc tggatacatg accgcgcgcg tactgatcgc tggttgcggc gatctcggcc
     2581 tgggggtcgt gtcggcgctc gcgcatgccc gattcgatat ccatggcctg cgccgcagcg
     2641 cacagccttt tcccaatggg atgacggcta tacaagccga tgtcactcgc ccagattccc
     2701 taggcgcact gcgaacactg cgccctgaca tcctgctcta ttgcgtcgcc gccgacgcgc
     2761 agaccgacga cagctatcgc acgcattatg tcgatggcct gcgcaacgtc ctggctacac
     2821 tcgaccccgc ccagctcagg catgtctttt tggtttc
//
LOCUS       DATMTW010000176         8671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_283077, whole genome shotgun sequence.
ACCESSION   DATMTW010000176 DATMTW010000000
VERSION     DATMTW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283077"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1506
                     /gene="guaB"
                     /locus_tag="VN084_03265"
     CDS             46..1506
                     /gene="guaB"
                     /locus_tag="VN084_03265"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWT28508.1"
                     /translation="MRLLQKALTFDDVLLVPAHSNVLPREVSLATQLTRSIRLNIPLV
                     SAAMDTVTEAPLAIALAQEGGMGIIHKNMSAAAQASHVLRVKRFESGVVHDPVVIQPH
                     MTVRDVLSLTRQHKISGLPVVDGNKVVGIVTNRDLRFETNLDQSISNIMTPRDRLVTV
                     PEGANREEVVQLLHKHRLERVLVVNDAFELKGLITVKDIQKSSDHPLACKDEKGRLRV
                     GAAVGVGEGTEERVAALAEAGVDVIVVDTAHGHSQGVLDRVTWVKKHFPEVQVIGGNI
                     ATASAAKALVDAGADGVKVGIGPGSICTTRIVAGVGVPQISAVANVEEALRGSGVPFI
                     ADGGIRFSGDISKAIAAGAYSVMLGGMFAGTEEAPGEIELFQGRSYKSYRGMGSIGAM
                     EKGSSDRYFQDNNGNADKLVPEGIEGRVPYKGSVLAVIHQLMGGLRASMGYVGCATIE
                     EMRTRAEFVQITSAGMRESHVHDVQITKEAPNYHVE"
     gene            1513..2238
                     /locus_tag="VN084_03270"
     CDS             1513..2238
                     /locus_tag="VN084_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004891621.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWT28509.1"
                     /translation="MQPAQQRHVAESAILGGLLLVGLALLGYLIAQSIVNIKLMDRSV
                     EVKGLSEREVAADIAIWPITFNVADNDLSNLYQTVQQKNDSVVKFLEKNGFKAEEITI
                     STPSVVDRQAREYGNDGANARYRYTASSTVTVYSNQVDAVRQSMIKLVELGKQGIAIA
                     GDTYSTQFIYSKLNDIKPGMIEEATRNAREAAEKFAKDSASKLGKIKHANQGQFSIEN
                     RDSSTAHIKKVRVVSTVEYYLSD"
     gene            2287..3849
                     /gene="guaA"
                     /locus_tag="VN084_03275"
     CDS             2287..3849
                     /gene="guaA"
                     /locus_tag="VN084_03275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWT28510.1"
                     /translation="MTQKILILDFGSQYTQLIARRVREANVYCELHSWDVDEAFIKAF
                     KPAGIILSGGPNSVYEDETPRAHDIVFKLGIPVLGICYGMQTMASQLGGKVENAAKRE
                     FGYAEIRARGHSRLFLNIQDRSNDEGHGLLDVWMSHGDKVTELPAGFDIIASNASTPI
                     AGMADEKRGFYAVQFHPEVTHTRQGKSILSRFVHEICGCDRSWNMPDYVETAVNRIRD
                     EVGSDQVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDNGLLRLNEAEQVMQTFARNL
                     GVKVIHVDASEKFLGDLKGVSDPEAKRKIIGREFVEVFQQESAKLPQAKWLAQGTIYP
                     DVIESAGAKTKKAHTIKSHHNVGGLPDTLKLKLLEPLRELFKDEVRELGVALGLPHDM
                     VYRHPFPGPGLGVRILGEVKKEYADLLRRADAIFIEELRHAGWYEKTSQAFAVFLPVK
                     SVGVMGDGRTYDYVVALRAVVTSDFMTAHWAELPYELLGKVSNRIINEVRGINRVVYD
                     ISGKPPATIEWE"
     gene            3965..4462
                     /locus_tag="VN084_03280"
     CDS             3965..4462
                     /locus_tag="VN084_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HWT28511.1"
                     /translation="MSRGFVKEDDLEHAGTDLPERPVSPHPNYVTPTGLQQLQQLAAD
                     LEQQRLPLTSRKEDPNAQQQLARINRDLRYVQARLESAILVDPAQQSGEQVLFGAHVT
                     VEDENGDTHVFMIVGEDEADINANKVSWVSPLAKSLLGHKVGESVTWLRPAGNLSLEI
                     INLDS"
     gene            4475..5077
                     /locus_tag="VN084_03285"
     CDS             4475..5077
                     /locus_tag="VN084_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWT28512.1"
                     /translation="MNVSTAIETRRSVKAFDAQHRMTQAEIDQLMSLAMLAPTAFNIQ
                     NWRFVLVQDPELRKQVRAVSWDQAQVTDASLLVVLVADLKSWDREPARYWKNAPQPVQ
                     DFLVPAIDTYYRGKEQVQRDEGMRSCGMAAMTLMLAAKEMGYDSCPMDGFDFDAVAKL
                     LNLPADHTPAMYVAIGKGIKEAWPRGGQLPMSEVVIRDKF"
     gene            5254..6849
                     /locus_tag="VN084_03290"
     CDS             5254..6849
                     /locus_tag="VN084_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HWT28513.1"
                     /translation="MLISNNITMQFGSKPLFENVSVKFGDGNRYGLIGANGCGKSTYM
                     KILAGVLEPSAGNVTLDNHERMAFLKQDQFAYEDQRVLDVVMMGHEEMWRVNQEKNAI
                     YMNPEATEDDYMKAAELEALFAEYDGYTAESRAGELLLGVGIPTEQHTGPMSAIAPGW
                     KLRVLLAQALFANPDILLLDEPTNNLDINTIRWLEDVLNNRDSTMIIISHDRHFLNQV
                     CTHTCDMDYGKLTVYPGTYDDYMEAAQQARAQQAKDNAKAKQQIADLQEFVRRFSANA
                     SKAKQATSRAKQIEKIKVEEFKPSSRQYPFIRFEYDDREKLHRQAVEIEKLSHGYDKP
                     LFNDFNLMVDAGEKIAIIGENGIGKTTLLRCIAGEIKPNHGTVKWAEKAKLGYFAQDH
                     EYEFENGETLFDWMTRFTQAGDDDQAVRSMLGRLLFSGEDTRKSVKVLSGGEKGRMLY
                     GKLMLARTNVLLMDEPTNHMDMESIEALNTALERYKGTLFFVSHDREFVSSLATRILE
                     IKPDGIVDFHGNYEDYLTSQGID"
     gene            complement(6887..7924)
                     /locus_tag="VN084_03295"
     CDS             complement(6887..7924)
                     /locus_tag="VN084_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWT28514.1"
                     /translation="MAKPEPRRWQGYALLAAGALLALGFLLGHYNSAHGHGDQRTRLL
                     TVGGALSQKFNADLQHTIDSIEATAWLIHANRELDRDTFQRFAEQALQSNPDIRELRW
                     SPRVGHADRSAFVARMREQGLADFDVTEERPGSGKRIVAARRDEYFPVLYTAPMRMSA
                     LGLDTYSLTDDRWAMHAALEQRKPFASSVQAHARQPRMMTIHVPVFAQGEAAGESASA
                     ANLVGYVTGVVTLDRLLTNMRQEVAELDADLLLLDSTNKTEQLLAYLPGRGGRVTSDE
                     AATEYRILKSGISLPVAVAGRQWMFVMQPRSGALGEDMLYIGILLISLLLTGLLVIWI
                     IRGRMDRVTEQ"
     gene            8060..>8671
                     /locus_tag="VN084_03300"
     CDS             8060..>8671
                     /locus_tag="VN084_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWT28515.1"
                     /translation="MKSKLTTMPAQQHPSRTALPDILQFAAVALALSLISTQAQSATE
                     PSLWYDNGDPFDTESLTPPVVPDGYLDSQHSPCTRPDYSAALTLADVANTTLCNNPQT
                     REAWANARVQAAQLGIARSAYLPSLTDNVATSMSYANPELSTRSNPYNNLSNSLVANY
                     LLYDFGNRNANLESARQLLQAAAATQNSTVQNLLLSAIQSYYQT"
BASE COUNT         1914 a         2715 c         2483 g         1559 t
ORIGIN      
        1 caaagcccta acccggtata attgcctttt gccggaaaaa tctctatgcg tctgttgcaa
       61 aaagcgctta ccttcgacga tgtcctactg gttccagcgc actccaacgt actcccccgt
      121 gaagtcagcc tagccaccca actcacccgc tccatccgtc tcaatatccc gctggtctcg
      181 gccgcgatgg ataccgtgac cgaagcgccg ctggccatcg cgctggcaca ggaaggcggc
      241 atgggcatca tccacaagaa catgtcggcc gcagcccagg ccagccatgt cctgcgcgtg
      301 aagcgcttcg agtccggtgt cgtgcatgat ccggtcgtca tccagccgca catgacggta
      361 cgcgatgtgt tgagcctgac gcgccagcac aagatctcgg gcctgccagt ggtcgacggt
      421 aacaaggtgg tcggcatcgt caccaaccgc gacctgcgct tcgagaccaa tctggatcaa
      481 tcgatctcca atatcatgac gccgcgcgac cgcctggtca ccgtgcctga aggtgcgaac
      541 cgtgaagagg tcgtgcagtt gctgcacaag caccgtctgg aacgcgtgct ggtcgtcaat
      601 gacgctttcg agctcaaggg tctgatcacg gtcaaggaca tccagaaatc cagtgaccac
      661 ccgctcgcct gcaaggatga gaaggggcgc ctgcgggtcg gtgccgccgt cggcgtaggt
      721 gaaggcacgg aagaacgcgt cgccgccctc gccgaagccg gtgtcgacgt catcgtcgtc
      781 gataccgcgc acggccactc gcaaggcgtg ctagaccgcg tcacctgggt caagaagcat
      841 ttccctgaag tacaggtcat cggcggcaac atcgccactg ccagcgccgc caaagccctg
      901 gtcgatgccg gtgcggacgg tgtcaaggtc ggcatcggcc ctggctccat ctgcaccacg
      961 cgtatcgtcg ccggtgtcgg cgtgccgcag atcagcgccg tcgccaacgt cgaggaagcc
     1021 ttgcgcggca gcggcgtacc gttcatcgcc gatggcggta tccgcttctc cggcgacatc
     1081 tccaaggcga tcgccgctgg cgcgtattcc gtgatgctgg gcggcatgtt cgccggcacc
     1141 gaggaagcgc caggcgagat cgaactgttc cagggtcgtt cctacaaatc ctaccgtggc
     1201 atgggttcca tcggcgccat ggaaaagggt tccagcgatc gctacttcca ggacaacaac
     1261 ggcaatgccg acaagctggt gccggaaggc atcgaaggcc gcgtacccta caagggcagc
     1321 gttctggcgg tgatccacca gctgatgggt ggcctgcgcg cctccatggg ctacgtcggc
     1381 tgtgcgacca tcgaagagat gcgcacgcgt gccgagttcg tgcagatcac ctcggccggt
     1441 atgcgcgagt cgcatgtgca tgacgtgcag atcaccaaag aagcgccgaa ctaccacgtc
     1501 gaataagcgc gcatgcagcc agcccaacaa cgccatgtcg ccgaatccgc catcctgggc
     1561 ggcttgttgc tcgtcggcct ggccttgctc ggctacctga tcgcgcagag catcgtcaac
     1621 atcaagctca tggaccgctc ggtcgaggtc aagggcctct ccgagcgtga agtggccgcg
     1681 gatatcgcga tctggccgat caccttcaac gttgccgaca acgacctgtc caacctctac
     1741 cagacggtgc agcagaagaa cgacagcgtc gtgaagttcc ttgaaaagaa cggcttcaag
     1801 gcagaagaga tcaccatctc gacgccatcc gtggtcgaca ggcaggcacg cgagtacggc
     1861 aacgatggcg cgaatgcccg atatcgctac accgcgagct cgaccgtcac ggtctacagc
     1921 aaccaggtgg atgccgtgcg tcaatccatg atcaagctcg tggaactggg taagcaaggc
     1981 atcgccatcg ctggtgatac ctacagcacc cagttcatct acagcaagct caacgacatc
     2041 aaaccgggca tgatcgagga agccacccgc aacgcgcgcg aagcggctga gaagttcgcc
     2101 aaggattccg cgagcaagct cggcaagatc aaacacgcca accagggcca gttcagtatc
     2161 gagaaccgcg actccagcac ggcgcacatc aagaaggtgc gcgtcgtgtc caccgtcgag
     2221 tattaccttt ccgactaatc caaaccgcgc cgccatcgct ggcggcccta ttgcctgaaa
     2281 ccatccatga cccagaaaat cctcatcctc gatttcggtt cccaatacac gcaactgatc
     2341 gcgcgccgcg tacgcgaagc caatgtctac tgcgaactgc attcgtggga tgtcgacgaa
     2401 gccttcatca aggcattcaa accagccggc atcatccttt caggcggccc gaattcggtc
     2461 tacgaggacg agacgccgcg tgcgcacgac atcgtgttca agctgggcat ccccgtcctc
     2521 ggcatctgct acggcatgca gaccatggcc tcgcagcttg gcggcaaggt cgagaacgcc
     2581 gccaagcgtg aattcggcta tgccgagatc cgcgcgcgcg gccactcgcg cctgttcctc
     2641 aacatccagg accgcagcaa cgatgaaggc cacggcctgc tcgatgtctg gatgagccat
     2701 ggcgacaagg tcaccgagct gccggccggc ttcgacatta tcgccagcaa cgcctcgaca
     2761 ccgatcgccg gcatggccga cgagaaacgt ggcttctacg ccgtgcagtt ccacccggaa
     2821 gtcacgcaca cgcgccaggg taaatccatc cttagccgct ttgtccatga gatctgcggc
     2881 tgcgaccgta gctggaacat gccggattac gtcgaaacgg cggtcaatcg catccgcgac
     2941 gaagtcggca gcgaccaggt catcctcggc ctgtcgggag gcgtcgactc atccgtcgcc
     3001 gcggcgctga tccaccgtgc gatcggcgac cagctgacct gcgtcttcgt cgataatggc
     3061 ctgctgcgct tgaacgaggc cgaacaggtg atgcagacct tcgccaggaa ccttggcgtc
     3121 aaggtgatcc atgtcgatgc cagtgagaaa ttcctcggcg acctcaaggg ggtcagcgac
     3181 ccggaggcca aacgcaagat catcggccgc gagttcgtcg aagtgttcca gcaggaatcc
     3241 gccaaactgc cgcaagccaa gtggctcgcg caaggtacga tctacccgga tgtgatcgaa
     3301 tccgccggcg cgaagaccaa gaaggcccac accatcaaga gtcaccataa cgtcggcggc
     3361 ttgcctgata cgctcaagct gaaattgctc gagccgctgc gtgaactgtt caaggatgaa
     3421 gtacgcgaac tcggcgtggc gctcggcctg ccgcatgaca tggtctatcg ccatcccttc
     3481 cctggtccag gcctgggcgt gcgcatcctc ggcgaggtga agaaggaata tgccgatctg
     3541 ctgaggaggg ctgacgccat cttcatcgag gaattgcgtc atgccggctg gtacgagaag
     3601 acctcgcagg cctttgccgt cttcctgccc gtgaagtcgg tcggtgtcat gggcgatggc
     3661 cgtacctatg attacgtggt cgcgctgcgc gccgtcgtca ccagcgactt catgaccgcg
     3721 cactgggctg agctgccgta cgaattgctg ggcaaggtgt cgaaccgcat catcaatgaa
     3781 gtacgcggca tcaaccgcgt ggtgtatgac atttccggca agccgccagc cacgatcgag
     3841 tgggagtaaa gcttttgcag gggtagcagg ttccgtcgac gcagtcgatg gatgcgaccc
     3901 ggaaaaagcg cccggccatg tcaattacag ccctcacaat ttcagcaatc ataagcgaag
     3961 ttaaatgagc cgcggtttcg tcaaagagga cgacctcgaa cacgccggca ccgatctgcc
     4021 ggaacggccg gtcagcccac accccaacta tgtgacgccc actggcctgc aacagctgca
     4081 gcagttggcc gcggatctgg agcagcagcg gctaccgctg acgagccgca aggaagaccc
     4141 gaacgcgcag caacagctgg cacgcatcaa ccgcgatttg cgctatgtgc aggcgcgact
     4201 tgagtcagca attctggtcg atccggcaca gcagtccggc gagcaggtac tgttcggcgc
     4261 tcatgtcacg gttgaggacg agaacggcga tacgcatgta ttcatgatcg ttggcgagga
     4321 tgaagccgac atcaacgcca acaaggtgag ctgggtttcg ccgctggcaa aatcgctgct
     4381 gggtcacaaa gtcggcgaga gcgtgacctg gttgcgcccg gcaggtaacc tgtcgctgga
     4441 aatcatcaac ctcgattctt aagaggaata tgccatgaac gtcagtaccg ccattgaaac
     4501 ccgccgctcc gtcaaggcgt tcgatgccca gcatcgcatg acgcaggccg agatcgacca
     4561 gctcatgtcg ctcgccatgc tcgcccccac cgcgttcaac atccagaact ggcgtttcgt
     4621 gctggtgcag gaccccgagc tgcgcaagca agtgcgtgct gtgagctggg accaggcaca
     4681 agtgaccgac gcatccctgc tggtggtgct ggtcgccgac ctcaagtcct gggaccgcga
     4741 gcccgcacgc tactggaaga atgcgcctca accggtgcag gatttcctgg taccggccat
     4801 cgacacctat taccgcggca aggaacaagt gcagcgcgac gaaggcatgc gctcctgcgg
     4861 catggctgcc atgacgctca tgctggccgc gaaggaaatg ggttacgact cctgccccat
     4921 ggacggtttc gacttcgatg ccgtcgccaa actgctcaac ctgccggctg accacacgcc
     4981 ggcgatgtat gtcgccatcg gcaagggcat caaggaagcc tggccacgcg gcggtcaatt
     5041 gccgatgagc gaggtcgtca tccgcgacaa gttttaatac ccttttgcaa tccgagccct
     5101 ttagcaatcg ccacgagcgg ttcatgatgc aaggcgcagc ggaatcgata cacaagacct
     5161 acccaagcgg taagcaggcg cattgaagaa ccgacagcgc cgcagatgga tcgcggggtt
     5221 gattgatata gggcttttca ccaggaacac tgaatgctaa tcagcaataa catcaccatg
     5281 cagttcggct ccaagccgct gttcgagaat gtctccgtca agttcggcga cggcaaccgc
     5341 tacggcctca tcggcgccaa cggctgcggc aagtcgacgt atatgaagat cctcgccggc
     5401 gtgctcgaac cgagtgctgg caacgtcacg ctcgacaacc acgagcgcat ggctttcctc
     5461 aagcaggacc agttcgccta tgaagaccag cgtgtgctgg atgtggtcat gatgggtcac
     5521 gaggagatgt ggcgggtgaa ccaggaaaag aacgccatct acatgaaccc ggaagcgacc
     5581 gaggacgatt acatgaaggc ggcagagctg gaagccctgt tcgccgaata tgacggctac
     5641 accgctgagt cgcgcgccgg tgagttgctg ctgggcgtag gtatccctac cgagcagcac
     5701 accggtccga tgagcgccat cgcgcccggc tggaaactgc gtgtactgct tgcgcaggca
     5761 ctgttcgcca acccggatat cctgctgctt gacgagccga ccaacaacct cgacatcaac
     5821 accattcgct ggctggaaga cgtgctcaac aatcgcgaca gcaccatgat catcatctcg
     5881 cacgaccggc acttcctgaa ccaggtatgc acccatacct gtgatatgga ctatggcaag
     5941 ttgaccgtct atccgggcac ctatgacgat tacatggaag ccgcacagca ggcccgcgca
     6001 caacaggcca aggacaacgc caaggctaag cagcagatcg ccgacctgca ggagttcgtg
     6061 cgtcgttttt ctgccaatgc gtccaaagcc aagcaggcca ccagccgcgc caagcagatc
     6121 gagaagatca aggttgagga gttcaagcct tcgagccgtc agtacccgtt catccgtttc
     6181 gaatatgacg atcgcgaaaa actgcatcgc caggccgtag agatagaaaa gctcagccac
     6241 ggctatgaca agcctttgtt caacgatttc aatctgatgg tggatgccgg cgagaagatt
     6301 gccatcatcg gcgagaacgg tatcggcaag accacgctgc tgcgctgtat cgccggtgag
     6361 atcaagccga accatggcac cgtcaaatgg gccgagaaag ccaagctcgg ctatttcgcg
     6421 caggatcacg aatacgagtt cgagaacggc gaaacgctgt tcgactggat gacccgcttc
     6481 acgcaggctg gcgacgacga tcaggccgtg cgcagcatgc tggggcgctt gctattctcc
     6541 ggcgaagaca ccaggaaatc ggtcaaggtg ctgtcaggtg gcgaaaaagg ccgcatgctc
     6601 tacggcaaac tgatgctggc caggaccaac gtgctgctga tggacgaacc gaccaaccac
     6661 atggacatgg aatcgatcga agccctgaac actgcgctgg aaagatacaa gggcacgctg
     6721 ttcttcgtca gccatgaccg tgagttcgtc agttcgctgg cgacgcgcat cctggagatc
     6781 aagccggacg gtatcgtcga tttccatggc aattacgaag attacctcac cagccaaggc
     6841 atagactgag ccaagggatc gacttcgact gaggctggcc agccgcttat tgctccgtga
     6901 cgcggtccat gcggccgcgg atgatccaga tcacgagcag gccggtgagc agcaggctga
     6961 tcagcaggat gccgatatac agcatgtctt cgcccaaggc gccgctgcgc ggctgcatca
     7021 cgaacatcca ctggcgaccg gccaccgcta ccggtagaga aatgccgctt ttcaggatgc
     7081 gatattcagt ggccgcttcg tccgacgtca cccggccgcc tcggcccggc aggtaagcca
     7141 gaagctgctc ggtcttgttg gtgctgtcaa gcaacaacag atccgcatcc agctccgcga
     7201 cctcttgccg catattcgtg agtaaacgat ccagggtgac cacgccggtc acataaccga
     7261 ccagatttgc cgcggacgcc gactcgcccg cagcttcccc ctgcgcgaac acaggcacat
     7321 ggatggtcat catccgtggc tgtcgggcat gcgcctgaac ggacgaggcg aacggtttgc
     7381 gttgttcgag cgcggcgtgc atggcccagc gatcatcggt caggctgtag gtatccaaac
     7441 ccagcgccga catccgcatc ggcgccgtgt agagcaccgg gaaatattca tctcgccgtg
     7501 cagcaacgat acgcttcccg ctcccgggcc tttcttccgt gacgtcgaaa tccgccagtc
     7561 cctgttcacg catcctggcg acgaaagccg aacgatcagc atggccaacc ctgggcgacc
     7621 agcgcaattc acggatgtcc ggattcgatt gcagtgcctg ttcggcgaag cgctggaagg
     7681 tgtcgcggtc aagctcgcgg ttcgcgtgga tgagccaggc ggtcgcttcg atgctgtcga
     7741 tcgtatgctg caggtccgca ttgaatttct ggctcaacgc accgcctacg gtcagcagtc
     7801 gcgtgcgttg atcgccgtgc ccatgggcgc tgttgtaatg gccgagcaga aagccgagcg
     7861 ccagcaaggc acccgcggcc agcaaagcat agccctgcca acgacgcggt tcaggttttg
     7921 ccatacatag caggtttagc cataagtagc gtcgctgcgt cccatttgta ttgttctttc
     7981 gttgattcta tatatgccgc gtcgcaaaat cactacaatt catgtcatcg ttgcggcagt
     8041 gcccaacgtt actgaccgag tgaaaagcaa actgacgacc atgccagccc agcagcatcc
     8101 atcccgaacc gcccttcccg acatcctgca gtttgctgca gtcgcactgg cgctatcgct
     8161 gatcagcaca caagcacagt ccgctacaga acccagcctg tggtacgaca acggcgatcc
     8221 gttcgatact gagtcgctga cgccaccagt ggttcccgat ggctacctgg acagccagca
     8281 ttcgccttgc acacgacccg attacagcgc tgcgctgaca ctggccgatg tcgccaacac
     8341 cactctctgc aacaatccgc agacccgcga agcctgggcc aacgcacggg tgcaggcggc
     8401 acagcttggt atcgcacgtt ctgcctacct gccctcgctg accgacaatg ttgcaactag
     8461 catgagctat gccaacccgg aactgtcgac ccgcagcaac ccctacaaca atctcagcaa
     8521 cagcctggtc gccaattacc tgctctacga cttcggcaac cgcaacgcca atctcgagag
     8581 cgcgcggcaa ttgctacagg ccgcggccgc gactcagaac agcacggtac agaacctgtt
     8641 gctgagcgcc atccagtctt attaccagac c
//
LOCUS       DATMTW010000177         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_283143, whole genome shotgun sequence.
ACCESSION   DATMTW010000177 DATMTW010000000
VERSION     DATMTW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283143"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2035)
                     /locus_tag="VN084_03305"
     CDS             complement(170..2035)
                     /locus_tag="VN084_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWT28516.1"
                     /translation="MSDTAPSRKSLSSLDRLWPFLKPYRWQLLLAFVLLSTASATLLL
                     VPLAFRDLIDFGFGQAPKAASAALANKSGASLNSHFAVLFGLACFWAVTVAARYLTVS
                     WIGERVTADLRTAVYSSVLGQSPQFFETLQTGEVLSRLTGDTTLIQTVIGSSASMGLR
                     SLFQFIGGMIMLAVTSAYLFSINLGLMLALTIPILLIGRSVRKLSRESQDRIADSSAL
                     AGEILNAMPTVQSYTQEQREIGRFRGTTEISFQTAIRRVKIRATMTAFVITAVMGAII
                     FVLWLGARQVADGSMTGGQLASFILYAAIVAGSVGTIAEVWGDLMRAAGATERLLELL
                     HAQPAVQAPAVPETPRNLNEALIEYRAVTFHYPSRLQVAALDKIDLSIAPGETVALVG
                     PSGAGKTTMLQLLLRFYDVTDGEIAINGQDIRKLSLESLRSMIAVVPQDPVIFSANAL
                     ENIRYGRPYATDAEVMLAAKAAQVDEFIHRLPDGYATFLGERGTRLSGGQRQRIAIAR
                     AMLKDAPILLLDEATSALDAESEILVQQGLNIAMQGRTTLIIAHRLATVQKADRIIVM
                     EDGRIVETGTPAELRNQSGLYARLASLQFDQRVVEDAGTEAGGMDTATEASTLGD"
     gene            2361..2519
                     /locus_tag="VN084_03310"
     CDS             2361..2519
                     /locus_tag="VN084_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HWT28517.1"
                     /translation="MSITTILLIILILMLIGALPTWGYSSGWGYGPSGGLGLVVLILL
                     ILLLTGRI"
     gene            complement(2546..3886)
                     /locus_tag="VN084_03315"
     CDS             complement(2546..3886)
                     /locus_tag="VN084_03315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT28518.1"
                     /translation="MKPIPKLRNAWAAALVVFMISVILVEFMVHSEFEKIRSKTDLDA
                     IAYGNTLRTRVDRELNKLLYIGVGLESYLHVYHTSLERDKITQILADLYLRGRHLRNI
                     AVAIGYTVTYVFPLQGNEKVLGMNYPDLTAQWPQVKQAVDSGKGVLVGPVDLVQGGSG
                     LIYRYPVFTQDGYWGMISIVIDTPSFMEAAFHGTRGKGYDFAVRNRDASGKVGKVFYG
                     DPALFASHDAVLMNSEVPDGTWQWAIKREAAPAYDLVWIIRLMGWLLSLAFAMSTYSF
                     FRERTQLANQALFDSLTGLANRRLLNDRLQQTIRRLEREKDQHCAVFFFDLDDFKTIN
                     DRHGHAAGDEVLMRVAQGVSEELRLSDTVGRLGGDEFVVISSYAHNPQHVTQIERRLR
                     KGIGKPITYNGHQFKIGASIGVAIYPEDASTAEALLALADQRMYENKQRRKRRD"
     gene            4104..>5449
                     /gene="mqo"
                     /locus_tag="VN084_03320"
     CDS             4104..>5449
                     /gene="mqo"
                     /locus_tag="VN084_03320"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HWT28519.1"
                     /translation="MAERTVDVLLVGGGVMSATLATLLVQLDPTLKIAMVERLDEVAR
                     ESTDGWNNAGTGHAGYCELNYTPQNAEGEVSIDRALGINAAFEVSLQYWSHLVEQGML
                     PTPEQFINPTPHESFVWGAQDVEFLRKRHQLLSRHHLFKDMQFSDDPTRIAEWMPLVM
                     AARDRGHPVAATRVDYGSDVDFGSLTRHMIQTLQAQPTFELLLGQAVKDLRRDRQGRW
                     QVRLQGRTSRKRQTLHADFVFLGAGGGALPLLQKSGIPEARGYGGFPVSGQWLVCHNP
                     DIVRQHRSKVYGKAPVGAPPMSVPHLDTRIIDGEPALLFGPYAGFTTKFLKQGSLLDL
                     ATSVRAGNLKPIVKAGLHNLDLTRYLVSEVFQSQKTRLASLRMFYPEARSRDWKLATA
                     GQRVQIIKHCDDKGGKLEFGTEIVAARDGSLAALLGASPGASTSVQAMIEVLERCFG"
BASE COUNT         1037 a         1584 c         1749 g         1079 t
ORIGIN      
        1 gagtgggcgc ggcatgttgt tgtttttcct gattgaattg ctgcgtttga gcatgccacc
       61 cacataaagt tcggacatac agctcggccg taaagtccgg acgttccatg tgccggcata
      121 tcggcctccg gcgcgccagg aagctgtgtc gaccggtgtt tcgagcgcgc tagtcgccta
      181 aggtacttgc ctctgtcgcc gtgtccatcc cgcccgcttc agtgccggca tcttcgacaa
      241 cacgctggtc gaattgcagg cttgcaaggc gggcatacag acctgactgg ttgcgaagct
      301 cggcaggggt gccggtctcg acgatgcgac cgtcttccat gacgatgatg cggtcagcct
      361 tttgtaccgt cgccaggcgg tgggcgatga tcagcgtggt ccggccttgc atggcgatat
      421 tcagcccttg ctgcacgagg atctcggatt cggcatcgag tgcgctggtg gcctcgtcca
      481 gcaacaggat cggcgcgtcc ttgagcatgg cgcgggcgat ggcgatacgc tggcgctggc
      541 cgccggacag ccgtgtgccg cgttcgccga gaaaagtcgc atacccatcg ggcaaacgat
      601 ggatgaattc gtcgacctgt gccgccttgg ctgccagcat gacttcggcg tcggtggcat
      661 aggggcgacc atagcggatg ttctccagcg cgttggcgga gaagatgacc gggtcctgcg
      721 gtaccacggc gatcatgctg cgcagggatt cgaggctgag cttgcggata tcctggccgt
      781 tgatggcgat ctcgccgtcg gtgacatcgt agaagcgcag cagcagttgc agcatggtgg
      841 tcttgccagc cccggacgga ccgaccaagg ccaccgtctc gccgggcgcg atgctgaggt
      901 cgatcttgtc cagtgccgcc acctgcaagc gtgacgggta atggaacgtg accgcacggt
      961 attcgattag cgcttcgttg aggttgcgtg gcgtctctgg caccgccggc gcctgcaccg
     1021 ccggttgcgc atgcagcagt tccagcaggc gttctgtcgc acccgccgca cgcatgaggt
     1081 cgccccagac ctcggcgatg gtgccgacac tgccggcgac gatcgcggca tacaggatga
     1141 aggaagccag ttggccaccg gtcatgctgc cgtccgcgac ctggcgcgcg ccgagccaca
     1201 gcacgaagat gatggcgccc atgacggcgg tgatgacgaa cgcagtcatc gtggcgcgga
     1261 tcttcacgcg gcggatggcc gtctggaaac tgatctcagt cgtgccacga aagcgcccga
     1321 tctcgcgctg ctcctgggtg tatgactgca cggtgggcat cgcgttgagg atctcgcccg
     1381 ccagcgccga actgtcggcg atgcggtcct gggattcgcg cgacagtttg cgtaccgatc
     1441 gtccgatgag caggatgggg atggtcaggg ccaacatgag accaagattg atcgagaaca
     1501 ggtaggcgct ggtgacggcg agcatgatca tgccaccgat gaactggaac aggctgcgca
     1561 ggcccatgga ggcagagctg ccgatgacgg tctggatcag ggtggtgtcg ccggtaagac
     1621 gcgacaggac ttcaccggtc tgcagggtct cgaagaattg cggtgattgg cccagcacac
     1681 tgctgtagac cgctgtgcgc aagtctgcgg tgacccgctc gccgatccag gagaccgtga
     1741 gatagcgggc cgcgaccgtc accgcccaga agcaggccag gccgaacaac actgcgaaat
     1801 ggctgttgag gctagcgccg gacttgttgg cgagcgctgc gctggcggcc ttgggtgcct
     1861 ggccgaagcc gaagtcgatg aggtcacgaa aggccagcgg caccaggagt agcgtggccg
     1921 aggcggtcga cagcaagacg aacgccagca gaagctgcca gcggtaaggc ttcaggaaag
     1981 gccatagccg gtcgagcgaa gaaagggatt tcctggaggg tgcagtatct gacatggagg
     2041 cgatctagag tccgaggcga atgacagtat agggcagacc gcgcgccgcg cgcgttcgtt
     2101 cacttgtcgg cggaggctga caccagaatc ggctccttgc cgataagcgt cgtgtgcgcg
     2161 acggcataca gtaagtcacg ctccactccg atcctgatcc ataaacaaaa cgcaaggagg
     2221 aaaaaaatga tcaagtactt tgtcgcgtgg cttctcggcg ttccggcgct ggtcctggtc
     2281 gtgatctatt tcttcatgaa ctgatcaccc tgaccatcca ttccgtccaa tcaaagtgtg
     2341 aagcacaata aggagacatc atgtccatca cgaccattct gctcatcatc ctgatcctca
     2401 tgctgatcgg cgctttgccc acttggggtt acagtagcgg ctggggttat ggtcccagtg
     2461 gcggcctagg ccttgtcgtt ctgatcctgc tgatcctgtt gctcaccggt cgcatctgac
     2521 ggcatcggtc agcttgccgc aagcgtcaat cccgacgctt gcggcgttgc ttgttctcat
     2581 acatgcgctg atcggcgagc gccagtagcg cttcggcggt cgacgcatcc tccgggtaga
     2641 tggcgacccc gatggatgca ccgatcttga attgatggcc gttgtaggtg atcggtttgc
     2701 cgataccctt gcgcaagcgg cgctcgatct gagtcacatg ctgcggattg tgggcatagg
     2761 acgagatcac gacgaactca tcgccgccga gccggcccac cgtgtcgctc aatcgcaact
     2821 cctcgctcac gccctgagcc acgcgcatca gcacttcatc gccggccgca tggccatggc
     2881 ggtcgttgat ggtcttgaaa tcgtccagat cgaagaagaa taccgcacag tgctgatcct
     2941 tttcgcgttc cagccggcgg atggtctgtt gcaggcgatc gttgagcagg cgacggttgg
     3001 ccagcccggt gaggctgtcg aacagggcct ggttggcgag ttgggtacgt tcgcggaaga
     3061 agctgtaggt gctcatggcg aaggccaggc tcaatagcca gcccatgagg cggatgatcc
     3121 acaccaggtc ataggcgggc gcggcttcgc gcttgatcgc ccattgccag gtgccgtccg
     3181 ggacttcgct gttcatgagt acggcatcat gcgaggcgaa cagggccgga tcgccataga
     3241 agaccttgcc caccttgccg gacgcatccc ggttgcggac cgcgaagtca taacccttgc
     3301 cgcgtgtgcc gtggaacgcc gcttccatga acgaaggggt atctatgacg atggagatca
     3361 tgccccagta gccgtcctgg gtgaagaccg gatagcgata gatcaggcct gagccgcctt
     3421 gcaccagatc gaccggcccc accagcacgc ccttgccgct gtccacggct tgcttgacct
     3481 gcggccattg cgcagtcagg tcaggatagt tcatgccgag caccttctca ttcccctgta
     3541 agggaaagac ataggtgacc gtatagccga tggcgacggc gatattgcgc agatgacggc
     3601 cacgcaggta gaggtcggcg aggatctgtg tgatcttgtc gcgttcgagg ctggtgtggt
     3661 agacgtggag atagctctct aggccgacgc cgatatagag cagcttgttc agctcacgat
     3721 cgacacgcgt gcgcaacgta ttgccgtaag cgatggcatc gaggtcggtt ttgctgcgaa
     3781 tcttctcgaa ttcgctgtgc accatgaatt cgaccaggat gacgctgatc atgaacacga
     3841 ccagtgccgc cgcccatgcg ttgcgcagct tgggtattgg cttcaacagt gggctcggca
     3901 agatagaggg catgtctgcc atgataccca cgaatccccg ggatttacat acacatgagg
     3961 caggaataag cctagatttt acataggctt agattttaca gacggcgcga tcacgcatat
     4021 catgctgtcc gggccgtggc acgcgccgac ggcgaatccg ctgtattccc gaattacctg
     4081 ttgttgtaga ggtctggact gtgatggctg aacgtactgt agatgtgctg ctggttggcg
     4141 gcggagtgat gagcgcgacg ctggcgacgc tgctggtaca actggacccg accctgaaga
     4201 tcgcgatggt ggagcgcctc gatgaagtcg cgcgcgaaag taccgatggc tggaacaacg
     4261 ctggtactgg ccacgccggg tattgcgagc tcaattacac gccgcagaac gcagaaggcg
     4321 aggtcagcat cgaccgcgcg ctcggcatca acgctgcatt cgaagtttcc ctgcaatatt
     4381 ggtcgcacct tgtagagcaa ggcatgttgc cgacccctga gcagttcatc aatccaaccc
     4441 cccatgagag tttcgtctgg ggcgcgcagg atgtcgaatt cctgcgcaag cgccaccagt
     4501 tactgagtcg ccatcacctg ttcaaggata tgcagttctc cgacgatccg acgcgcatcg
     4561 ccgaatggat gccgctggta atggctgcgc gcgatcgcgg ccatcctgtc gcggcgacac
     4621 gcgtggatta cggcagcgat gtcgatttcg gttcgctgac gcggcacatg atccagacac
     4681 tgcaggcgca gccgacgttc gagttgctgc tggggcaggc agtcaaggat ctgcgccgcg
     4741 atcgtcaagg ccgctggcag gtgcgcttgc aaggccgtac cagtcgcaag cgtcagacac
     4801 tgcatgctga tttcgtcttc ctcggcgccg gtggtggcgc cttgcccctg ctgcagaaat
     4861 ccggcatccc ggaagccagg ggttacggtg gattcccggt gagcggccag tggctcgtct
     4921 gccacaatcc ggacatcgtt cgccagcatc gttccaaggt ctacggcaag gcgccggtcg
     4981 gcgcgccgcc catgtcggtg ccgcatctgg atacccgcat catcgacggc gagccggcgt
     5041 tgctgttcgg cccctatgcg ggattcacca ccaagttcct caagcagggg tcgctactgg
     5101 atctggcgac ttcggtcagg gctggcaacc tgaaaccgat cgtgaaggcc gggctgcaca
     5161 atctggatct gacgcgctac ctggtgagcg aggtgttcca gtcgcaaaag acgcgcctcg
     5221 cgtcgctgcg catgttctat cccgaagccc gttcgcgcga ctggaagctg gccactgcgg
     5281 gccagcgcgt gcagatcatc aagcattgcg acgacaaggg cggcaagctg gaattcggga
     5341 ccgagatcgt cgccgcccgg gatggatcgt tggcggcgct gctgggcgct tcgcctggcg
     5401 cctcgacctc ggtgcaggcg atgatcgaag tgctggaacg ctgtttcgg
//
LOCUS       DATMTW010000178         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_351309, whole genome shotgun sequence.
ACCESSION   DATMTW010000178 DATMTW010000000
VERSION     DATMTW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351309"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VN084_03325"
     CDS             complement(<1..743)
                     /locus_tag="VN084_03325"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWT28520.1"
                     /translation="MIAACSPIKTLNALVPEDGYLMRGDIAYGAEARQRLDVYRPRDA
                     GDSPMPVVVFFYGGGWEDGQRADYKFVAEALTSKGMVVVIPDYRVYPDAVFPTFIEDA
                     AKAVRWAKDHAADFGGDPSRLFLAGHSAGAHIAAMLALDAHYLKAEQLTPADLTGIIG
                     LSGPYDFLPLKSARLKQIFGPEAEWPKSQPINFVHGDNPPMLLLTGEDDGTVWPRNTY
                     RLAKKIEASHGEVEVGSYPGWGHADMVAKL"
     gene            978..1376
                     /locus_tag="VN084_03330"
     CDS             978..1376
                     /locus_tag="VN084_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HWT28521.1"
                     /translation="MTKRIALPQSCPCGIKQPYANCCERYHHGTPAPTAEALMRSRYT
                     AYVYGLEDHLLATWHPDTRPAELELAHEPTPKWLGLEVRHSAESSETTATVEFIARYK
                     VGGKAERLHETSRFERIDGRWYYRDGIQHS"
     gene            complement(1434..1991)
                     /locus_tag="VN084_03335"
     CDS             complement(1434..1991)
                     /locus_tag="VN084_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28522.1"
                     /translation="MNFSQRLCFLHALCLNETAQDVAAVNTNLEDYDPQDAAHYLACY
                     ITFRAIRHAQRSPADERVENFDMLGVYHAYAMLVYAFLMLPLASEDIAPDTESASVII
                     AKTLFAGLAEEEWAEIIESGSHKFQLIAEAEQEHWMTYRQDLDKSVVAFVVAATDEET
                     PFDKEEVIPMFGALLSMLCEAFASE"
     gene            complement(2072..>2785)
                     /locus_tag="VN084_03340"
     CDS             complement(2072..>2785)
                     /locus_tag="VN084_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT28523.1"
                     /translation="MLRLDQERNQPQAREQDHRNYLGGFDFRGDMASTPCGPFSGGEK
                     SRLALALMIWRQPNLLLLDEPTNHLDLEMRHALTLALQAFEGGVVLVSHDRALLRATC
                     DRFVLVVDGKATTFDGDLDDYKTWLAQQATRDAPEPQKKPEKNDRAQARADRQARLAE
                     RRPLVKESEKLEADMAKWQLERNGIDQRLTDNSLYAPEAKVELQGLLKRQGELMQAIE
                     HAEERWLAIHEQLEALPEI"
BASE COUNT          542 a          868 c          816 g          559 t
ORIGIN      
        1 agcttcgcca ccatgtccgc atgtccccaa cccggatagc tgccgacttc gacttcgccg
       61 tgactggcct cgatcttctt ggccagccgg taagtgttgc gcggccacac cgtgccgtca
      121 tcctcgcctg tgagcagcag catgggcggg ttatcgccat gcacgaaatt gatgggctgc
      181 gattttggcc attccgcttc cgggccgaag atctgcttga ggcgcgcgct tttgagcggc
      241 aggaaatcat aaggcccgga gaggccgatg atgccggtca ggtcagcggg tgtgagttgc
      301 tcggccttga ggtaatgcgc atcaagcgcg agcatcgcgg cgatgtgggc acctgcggaa
      361 tgtccggcaa ggaacaggcg gctcgggtcg ccgccaaaat cggcggcatg gtccttggcc
      421 cagcgcacgg ccttggcagc atcttcgatg aatgtcggga acaccgcatc cggatagacg
      481 cgataatcag gaatgacgac gaccatgcct ttcgaagtca gggcctcagc gacgaatttg
      541 tagtcggcgc gttggccgtc ctcccagcca ccgccataga agaacaccac cactggcatg
      601 ggagaatcgc ctgcgtcgcg cggccgatag acatccagac gttgccgcgc ctctgcgccg
      661 taagcgatgt cgccacgcat gagatagccg tcctcgggca ccagcgcatt gagcgtcttg
      721 atcggggaac atgcggcgat caagaccgcc gccgccagcg ccgccgcctg tttcgctacc
      781 ccatagaaaa atcgccgatc tgtccgcatc cgcccttgtc ctcagccgtt ggctgcatta
      841 ccctcgctac acgttgtgta acaaggcact tcgatcgatg cgcccatatc aatgcgccaa
      901 taccgatgca ttgatggatc cgccgccacg accaacgcca cgcagcccga atcagctagc
      961 aggatatccg aaagcccatg accaagagaa tcgcactacc ccaaagctgc ccctgcggga
     1021 tcaagcagcc ctacgcgaac tgctgcgagc gatatcacca cggcacccca gctccgaccg
     1081 cagaagcgct catgcgttcg cgctacacgg cctacgtcta tggactggaa gatcacctgc
     1141 tcgcgacctg gcaccccgac acccggccgg ccgaactgga actggcccat gagcccacgc
     1201 ccaaatggct gggtttagag gtcaggcata gcgcagaatc gtcggaaacg acggcgaccg
     1261 tcgaattcat cgcgcgctac aaggtgggcg gcaaggccga acggctgcat gaaaccagcc
     1321 gttttgagcg tatcgacggc cgttggtatt accgtgacgg catccagcac agctaaaggc
     1381 cgatcgccct tggtggcatc ttcaggcggg aattcggcgc cggacgcgcg ccttcactcg
     1441 ctggcgaaag cctcgcacaa catgctgagc aaggcgccga acatgggaat gacttcctct
     1501 ttgtcgaacg gcgtttcttc gtcggtagca gccacgacga aagcgacgac ggatttgtcc
     1561 agatcctggc gataggtcat ccagtgctcc tgctccgcct cggcgatcag ctggaacttg
     1621 tggctgccgg attcgatgat ctctgcccat tcttcttcag caaggcctgc gaatagcgtc
     1681 ttggcgatga tcaccgaagc tgattcggta tccggcgcaa tatcctcgct ggccagcggc
     1741 agcatgagga atgcatacac cagcatggca taggcatggt agacgccgag catgtcgaaa
     1801 ttctctaccc gctcgtctgc cggcgagcgc tgcgcgtggc ggattgcgcg aaacgtgatg
     1861 tagcaggcga ggtaatgcgc cgcatcctgc gggtcatagt cctcaaggtt ggtgttgacg
     1921 gcggcgacgt cctgcgcggt ctcgttcagg cacagcgcgt gcaggaaaca tagtcgttgt
     1981 gagaaattca tgcttgttcg ctttctgttg agaataattc attcaccgca gatgaacgca
     2041 gataaatgca ccgcgctcat cattcatcac ttcagatctc cggcaaggct tccaattgct
     2101 cgtggatcgc aagccagcgt tcttcggcat gttcgatcgc ttgcatgagc tcgccctggc
     2161 gcttgagcag cccctgtaac tcgaccttgg cttccggcgc atagaggctg ttgtccgtca
     2221 ggcgctggtc aatgccgttc ctttccagct gccattttgc catgtcggct tccagttttt
     2281 ccgattcttt caccagcggc cgccgttcgg cgagccttgc ctggcgatca gcccgggcct
     2341 gggcacggtc gttcttttcc ggctttttct ggggttctgg cgcgtctcgc gtcgcttgtt
     2401 gggcaagcca tgtcttgtaa tcatccagat cgccgtcgaa ggttgtcgcc ttgccgtcca
     2461 ccaccagcac aaagcggtca caggtggcgc gcagcaaggc acggtcgtgc gagaccagca
     2521 ccacgccgcc ttcgaaggcc tgcagcgcca gcgtcaaagc atggcgcatc tcgaggtcga
     2581 ggtgattggt cggctcatcg agcaagagca gattgggctg gcgccagatc atgagcgcca
     2641 gcgccagtcg cgacttctcg ccgccggaga atggcccgca aggggtgctc gccatatccc
     2701 cgcggaaatc gaatcctccg aggtaattgc ggtgatcctg ttcgcgcgcc tgaggctggt
     2761 tacgctcctg atcgagtcgc agcat
//
LOCUS       DATMTW010000179         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_83027, whole genome shotgun sequence.
ACCESSION   DATMTW010000179 DATMTW010000000
VERSION     DATMTW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83027"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="ccoN"
                     /locus_tag="VN084_03345"
     CDS             complement(<1..1422)
                     /gene="ccoN"
                     /locus_tag="VN084_03345"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWT28524.1"
                     /translation="MQEQAAYNYQVVRQFTVMTVVWGVVGMLVGVFIAAQLVWPDLNF
                     VEWLSYGRLRPLHTNAVIFAFGGCSLFATSYYCVQRTCHVPLFAPKLAAFTFWGWQLV
                     IVLAIISLPMGYTTAKEYAELEWPIDILIAAVWISYAVVFFGTIVKRKTPHIYVANWF
                     FGGYILTIALLHIVNSAEIPVSLTKSYSAYAGVQDAMVQWWYGHNAVGFFLTAGFLGM
                     MYYFVPKQAERPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMIFSLIL
                     LAPSWGGMINGIMTLSGAWHKLRDDPILKFMITSLSFYGMATFEGPMMSIKTVNALSH
                     YTDWTIGHVHAGALGWVAFIAIGSCYYLIPRMFGKTEMYSKRLIELHFWIATLGIVLY
                     IASMWIAGVMQGLMWGATNPDGTLTYSFVEGVKASYRFWEIRLLGGAMFLTGMLLMSW
                     NMAMTIRGGRAVNDAPVLAPAGAH"
BASE COUNT          364 a          450 c          522 g          262 t
ORIGIN      
        1 gtgggcgccc gccggtgcaa gcaccggggc gtcattgacg gcacggccgc cgcgtatggt
       61 catggccatg ttccagctca tcagcagcat gccggtgagg aacatggcgc cgccgagcag
      121 gcggatttcc cagaagcgat agctggcctt gacgccttcg acgaaggaat aggtgagcgt
      181 gccgtccgga ttggtggcgc cccacatcag gccctgcatc acgccggcga tccacatcga
      241 ggcgatgtag agcacgatgc cgagcgtggc gatccagaag tgcagttcga taaggcgctt
      301 ggaatacatc tcggtcttgc cgaacatgcg cgggatcagg taatagcagc tgccgatggc
      361 gatgaaggcg acccagccga gcgcgccggc atggacgtgg ccgatggtcc agtcggtgta
      421 gtgcgagagc gcgttcacgg tcttgatcga catcatcggg ccttcgaagg tggccatgcc
      481 gtagaaggac agcgaggtga tcatgaactt cagaatcggg tcgtcgcgca gtttgtgcca
      541 ggcacccgag agggtcatga tgccgttgat catgccgccc cacgacggcg ccagcaggat
      601 cagcgagaag atcatgccga gcgactgggt ccagtcgggc agcgcggtgt agtgcaggtg
      661 gtgcggaccc gcccacatgt acgtgaagat cagggcccag aagtgcacca ccgacaggcg
      721 gtaggagtac accgggcgtt cggcctgctt gggtacgaag taatacatca tgccgaggaa
      781 gcccgcggtg aggaagaagc cgaccgcatt gtggccgtac caccactgca ccatcgcatc
      841 ctgcacgccg gcgtaggccg agtaggactt ggtcagcgac accgggatct cggcgctatt
      901 gacgatgtgc agcagggcaa tggtgaggat gtagccgccg aagaaccagt tggcgacgta
      961 gatgtgcggc gtcttgcgct tgacgatggt gccgaagaag acgacggcgt aggaaatcca
     1021 gacggcggcg atgaggatgt cgatcggcca ctccagctcg gcgtattcct tggccgtggt
     1081 gtaacccatg ggcagcgaga tgatcgccag cacgatgacc agctgccagc cccagaaggt
     1141 gaaggcagcg agtttcggcg cgaacaaagg cacatgacaa gtgcgctgca cgcagtaata
     1201 ggacgtggcg aacagcgaac agccgccgaa ggcgaagatc accgcattcg tatgcagcgg
     1261 acgcaggcgt ccgtaggaaa gccattcgac gaaattgaga tccggccaga ccagctgggc
     1321 ggcgataaag acgccaacca gcatgccgac cacgccccac accaccgtca tgacggtgaa
     1381 ctggcgcacg acctggtagt tataagccgc ttgctcttgc atgctcatcc tcactcttgg
     1441 aatcaaccgg cgctccggaa tggagggcgc ccagactgat aagtggaaga taccgatgcc
     1501 gcaaatggcc gtcttgactc agatcaaatc ggcagcagtc ggaagatcga aacaggtatg
     1561 catcggggat acgctcctca atctagaaca tcacattc
//
LOCUS       DATMTW010000180         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_283772, whole genome shotgun sequence.
ACCESSION   DATMTW010000180 DATMTW010000000
VERSION     DATMTW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283772"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN084_03350"
     CDS             <1..275
                     /locus_tag="VN084_03350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWT28525.1"
                     /translation="LMGIEGLFDQIFSVESSGFHPKPSFRGFARMLQAIKARPEDCVM
                     LEDNPEALMTAKRLGMKTVLVSRRMHKPNYVDARIGNILELTRTTI"
     gene            297..854
                     /gene="slmA"
                     /locus_tag="VN084_03355"
     CDS             297..854
                     /gene="slmA"
                     /locus_tag="VN084_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HWT28526.1"
                     /translation="MAGERKQQILETLAKMLESPKREKITTASLAAKLDVSEAALYRH
                     FAGKAQMFEGLIEFIEQTLFGLINKITSEETDGLAQVQRVVTVLLMFAEKNPGMTRVL
                     IGDALVNEDDRLQVRINQLFDRLESTLKQSLRIAETQSGRKREPEAQANLILCYVIGR
                     WHQFAKSGFKRKPMEFAQLQLSALI"
     gene            941..>1887
                     /locus_tag="VN084_03360"
     CDS             941..>1887
                     /locus_tag="VN084_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWT28527.1"
                     /translation="MVHSIEESKRWLALIILCLGELMIVLDTTIVNVALPSIRNDLAF
                     TESALVWVVNAYMLTFGGFLLLGGRLGDLFGQRKLFLLGIILFTGASLACGIANSQTM
                     LIIARGIQGLGGAVVSAVALSLIMSLFTENGERAKAMGIYGFVCAGGGSVGVLLGGVL
                     TSMLSWHWIFLVNLPIGIAVYAVCLRILPPGEPQTDVRHMDIGGALTITASLMLTVYA
                     IVNGHAAGWLSPMTGSLLGASLLLFIAFLVIESRVHRPLVPLRFFRLRNVSIANVIAA
                     LWAAAMFAWFFISALYMQLVLGYDAMQVGLAFLPSNLIMA"
BASE COUNT          397 a          585 c          517 g          388 t
ORIGIN      
        1 aactgatggg tatcgaaggc ctgttcgacc agatattcag tgtggaatcc tcgggcttcc
       61 acccgaagcc ttcgttccgc ggattcgcac ggatgttgca ggcaatcaag gccagaccag
      121 aggattgcgt gatgctagaa gacaatccgg aagccctcat gaccgccaag cggctgggca
      181 tgaagaccgt acttgtcagc cggcgcatgc ataagccgaa ttatgtcgat gcccgtatcg
      241 gcaacatact cgaattgacc agaaccacga tctagaacaa gttaggaaca ataaaaatgg
      301 caggcgaacg caaacaacag atcctcgaaa ccctggcaaa gatgctcgaa tcgcccaaac
      361 gcgaaaagat caccaccgca tcgctcgccg ccaagctcga cgtctccgaa gctgccctct
      421 accgccactt cgccggcaag gcacagatgt tcgaaggctt gatcgaattc atcgagcaga
      481 ccctcttcgg cctcatcaac aagatcacca gcgaagagac cgatggcctg gcccaggtgc
      541 agcgcgtggt caccgtgttg ctcatgttcg ccgagaagaa cccgggcatg acgcgcgtac
      601 tcatcggcga cgcgctggtc aatgaggacg accgcctgca agtccgcatc aatcagttat
      661 tcgaccgtct ggaatccacc ctcaaacaga gcctgcgcat cgccgagacc caaagcggac
      721 gcaaacgcga acctgaagcg caagccaacc tcatcctctg ctacgtcatc ggccgctggc
      781 atcagttcgc caagagcgga ttcaagcgca agccgatgga gttcgctcag ctgcagttat
      841 ccgctctgat ctagggcgag gtcatctcgc ctatcctcca ttagccgcct ccccagaaaa
      901 tcggctttgc ggcacttgag ccaataaaac acgacgaacc atggtccact cgatcgaaga
      961 aagcaagcgc tggctggcgc tgatcatcct ctgcctcggc gaactcatga tcgtgctgga
     1021 caccacgatc gtcaatgtcg cactgccctc catccgcaac gacctggcat ttaccgaaag
     1081 cgccctggtc tgggtggtca acgcctatat gctgaccttc gggggtttcc tgcttctggg
     1141 cgggcggctg ggagacctgt tcggccagcg caagctcttc ctgctcggca tcatcctgtt
     1201 cacaggtgcc tcgctggcct gcggtatcgc caattcgcaa accatgctga tcatcgcgcg
     1261 ggggatacag gggttgggcg gcgctgtcgt atcggctgtc gccctttcgc tcatcatgag
     1321 cctgttcacg gaaaacggtg aacgcgccaa ggccatgggc atctatggtt tcgtatgtgc
     1381 gggtggcggc agtgtcggcg tactgctcgg cggtgtcctg accagcatgc tgagctggca
     1441 ctggatattc ctggtgaacc tgccgatcgg gatcgcggtg tatgcagtct gcttgcggat
     1501 attgccgccc ggagaaccac agaccgatgt caggcacatg gatatcggcg gagccctgac
     1561 catcacggcc tccctcatgc tgaccgtcta tgcgatcgtc aatggccatg ccgccggttg
     1621 gctatccccg atgacgggaa gcctgctggg agcgtccctt ctgttgttca tcgccttcct
     1681 cgtcatcgag tcgcgcgtgc acagaccact cgtccctttg cgattcttcc gactgcgcaa
     1741 cgtatccatc gccaatgtca tcgccgcgct atgggcagct gccatgttcg catggttctt
     1801 catctcggcg ctctacatgc agcttgtgct gggctatgac gcgatgcagg tcgggctggc
     1861 tttcctgcca tcgaacctga tcatggc
//
LOCUS       DATMTW010000181         7105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_418385, whole genome shotgun sequence.
ACCESSION   DATMTW010000181 DATMTW010000000
VERSION     DATMTW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418385"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1675
                     /locus_tag="VN084_03365"
     CDS             137..1675
                     /locus_tag="VN084_03365"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWT28528.1"
                     /translation="MKNEQLIIFDTTLRDGEQSPGAAMTKEEKIRIARQLEKLGVNVI
                     EAGFAAASPGDFEAIQAVAEIIQKSTVCSLARANENDVRRAGEAIKPAKHGRIHTFIA
                     TSPIHMENKLRMTPEQVLERAVQAVKWALEYTDDVEFSAEDAVRSDMDFLVQVFTAVI
                     DAGAKTINVPDTVGYSIPALWGERIKQLIERVPNADKVIWSTHCHNDLGMAVANSLAA
                     VMAGARQVECTINGLGERAGNASLEEIVMAVKTRRDIFRLDTTIDTTQIVPTSKLVST
                     ITGYPVQPNKAVVGANAFAHESGIHQDGVLKHRETYEIMSAQDVGWGANKLTLGKLSG
                     RNAFRTRLKELGIELDSEDALNAAFARFKALADKKSEIFDEDIQALVSDEILGEADDN
                     FKLVYLHVASQTGETPHAVVAVSDNGVEKRAEADGGGPVDAAFKAIENIVASGVELQL
                     YSVNNITSGTDAQGEVTVRLARGGRIVNGQGADTDIVIASAKAYLNGLNKLHAKFERA
                     HPQV"
     gene            1714..2130
                     /gene="rnk"
                     /locus_tag="VN084_03370"
     CDS             1714..2130
                     /gene="rnk"
                     /locus_tag="VN084_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HWT28529.1"
                     /translation="MLQGSIQVTSFDMSRLFAMIEQMRQHGVVEPASLARLEDELARC
                     DEVAAEAVDADVVTMNSCVELRDLASGQTHTLTLVFPQQADAAAHRVSVLAPLGTAIL
                     GYRVGDTIDWPMPAGVRQYQILAIRYQPEAAGDFHL"
     gene            2179..3090
                     /gene="yedA"
                     /locus_tag="VN084_03375"
     CDS             2179..3090
                     /gene="yedA"
                     /locus_tag="VN084_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HWT28530.1"
                     /translation="MSESTHPARLRLLVLLALFALYFIWGSTFLAMKFAIESFPPFMM
                     AAIRFSIAGGLLYGVLRLYGTPAPTGRQWLGASAVGILLLSVGNAGVAIAEQTVSSGV
                     AALSIATVPLWMALFSGVWGHWPSRREWTGIVVGTVGVAVLSTGGTLQASPMGTFLLL
                     LAAASWAFGSVWGKRLAMPQGAMASAAQMLAGGAMLLVASTVSGEHWPAVVSMKSVWA
                     IVYLIIFGSFIAYSAYLFLLKTVRPVLATSYAFVNPVVAMLLGAWLADEHIGRTESVA
                     LLIIIVGVLLVLPFKRNEPAKSPSAGP"
     gene            complement(3074..3739)
                     /locus_tag="VN084_03380"
     CDS             complement(3074..3739)
                     /locus_tag="VN084_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HWT28531.1"
                     /translation="MNFPDLLLPTNLLWAANAVALLVLLHSARQARWRILSSTMLNVF
                     LGFCVSLGVLWSIRAGIKPGLNFHVLGATVLTLMFGPYLALIALAMVLMLATAFGLSG
                     LESYGINFVLMALLPVLISHGLFRWADRKLPNHFFIYVLVDGFFAGAVAMAALGFASC
                     FILSEAGAYSSSYLHSEYLPFYMLMAWSEALLTGMAVTLMAVYRPHWLVTFDDQRYLK
                     VRR"
     gene            3828..4490
                     /locus_tag="VN084_03385"
     CDS             3828..4490
                     /locus_tag="VN084_03385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWT28532.1"
                     /translation="MQLALFDLDNTLLAGDSDFQWGQFLIEEGLLDRDEHHDRNVQFY
                     EDYKAGKLDIDAFLAFQLKPLSRHSKDALHALHRKYMERKVRPMMTDKARALVEKHKA
                     QGDLMLIITATNSFVTKPIATEFGIEHLIGTDPECVDGRYTGGVSGIPSFQQGKVTRL
                     HQWLEQRGQYLEDFEKSWFYSDSHNDLPLLKLVTDPVAVDPDPVLQAHAEQQGWPIIS
                     LR"
     gene            complement(4592..5398)
                     /gene="ccsA"
                     /locus_tag="VN084_03390"
     CDS             complement(4592..5398)
                     /gene="ccsA"
                     /locus_tag="VN084_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWT28533.1"
                     /translation="MIEFIPYLLTAVIYIWVAADIWFNPKTAASTTRLRWHALAIDTG
                     LALHGYVLYRGLFDGGFNLSFSNALSAIFWLTVLIYWVTDFRHRLYGLQAFVLPPAAF
                     FVLLQKLSPETHLLPYADQPMFLAHLFIAMLAYSLFTFAAMHALLMAVAERSLHQKPT
                     LLKLPDFPPLMTMETLLFRVISVGFLLLTLTLLSGMLFSEALFHQPLKFNHKNLFTIL
                     SWLIFGGLLLGRVAYGWRGRTAIRWTLIGFVLLLLAYAGSKFVLEILLHR"
     gene            5495..6847
                     /gene="ffh"
                     /locus_tag="VN084_03395"
     CDS             5495..6847
                     /gene="ffh"
                     /locus_tag="VN084_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWT28534.1"
                     /translation="MLENLSGRLQQVIKTLRGQARLTEENIADAMREVRMALLEADVA
                     LPVVKEFIAHVKERAQGREVLQSLTPGQAVIQVVHEELTKLMGEQNAGLDLATQPPAI
                     VLMAGLQGSGKTTTSAKLARLLKAEKKKVLLASADVYRPAAIEQLKTLAQSLEIDCFD
                     SNATQKPADIAAGAIDFAKRHFYDVLIFDTAGRLAIDEAMMAEIKQLHSLLNPIETLF
                     VVDAMQGQDAVNTARAFGETLPLTGIVLTKLDGDARGGAALSVRHITGKPIKFIGVSE
                     KVDGLEPFHPERMASRVLGMGDVLSLIEQAQKNVDLDEAKKLAEKVKSGKNFDLDDFK
                     AQMSQMRKMGGVGALMDKMPAQMAGMAGQMSGEAGDKAIRRIEGIINSMTPLERRKPD
                     IIKATRKRRIAAGAGVQVQEVNRLLNQFEEVQKMMKLFSKGGMGKMMRAMQGRFPGMR
                     "
     gene            6966..7040
                     /locus_tag="VN084_03400"
     tRNA            6966..7040
                     /locus_tag="VN084_03400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6998..7000,aa:Lys,seq:ttt)
BASE COUNT         1470 a         2102 c         2251 g         1282 t
ORIGIN      
        1 cgcactacgg cctccttctg gccggcctgc tgctgcgccc cgcgcgctaa ataaaccccc
       61 ccacaccggt agtacccaag cgccgcctcg cacgatgccc aggctggcag agacttatcc
      121 ataacggact aaagacatga aaaacgaaca attgattatt tttgatacca ccttgcgcga
      181 tggcgaacag agccccggcg cggcgatgac caaggaagag aagatccgca tcgcgcggca
      241 gctggagaaa ctcggcgtca acgtcatcga ggccggcttc gccgctgcga gccctggcga
      301 tttcgaggct atccaggcgg tggccgagat catccagaaa tcgaccgtgt gctcgctcgc
      361 gcgtgccaac gagaacgacg tgcgtcgtgc cggcgaagcg atcaagccag ccaagcatgg
      421 ccgcatccac accttcatcg cgacctcgcc catccatatg gagaacaagc tgcgcatgac
      481 gccggagcag gtgcttgagc gcgccgtgca ggcggtgaaa tgggcgctgg aatacacgga
      541 cgacgtcgag ttctcggctg aggacgccgt gcgttccgac atggatttcc tggtgcaggt
      601 attcacggct gtgatcgacg ctggcgccaa gaccatcaac gtgccggata ccgtcggcta
      661 ttcgatccct gccttgtggg gcgagcgcat caagcaattg atcgagcgcg tgccgaacgc
      721 cgacaaggtg atctggtcta cacactgcca taacgacctc ggcatggccg tggccaattc
      781 gctcgccgcc gtcatggccg gcgcgcgcca ggtcgagtgc accatcaacg gtctcggcga
      841 acgggccggc aacgccagtc tggaagagat cgtcatggcg gtgaagaccc gtcgcgatat
      901 tttccgtctc gacaccacca tcgacacgac gcagatcgtg ccgacctcca agctggtctc
      961 gaccatcacc ggctacccgg tgcagccgaa caaggccgtg gtcggcgcca atgcctttgc
     1021 gcatgaatcg ggtatccacc aggacggtgt gctcaagcac cgcgagacct acgagatcat
     1081 gagcgcgcag gacgtgggct ggggcgccaa caaactgacc ttgggcaagc tctcgggccg
     1141 caacgccttc cgcacgcgat tgaaggaatt gggtatcgag ctcgacagcg aagacgcatt
     1201 gaacgcggcg ttcgcccgtt tcaaggcatt ggctgacaag aaatcggaga tcttcgacga
     1261 ggatatccag gccctggtca gcgacgagat cctcggtgaa gccgacgaca acttcaagct
     1321 cgtctacctg cacgtcgcgt cgcagaccgg agagacgccg catgcggtgg tcgccgtctc
     1381 cgacaacggc gtcgagaaac gcgccgaagc cgatggcggc ggcccggtcg atgccgcttt
     1441 caaggcgatc gagaatatcg tcgccagtgg cgtcgagttg cagctgtact cggtcaataa
     1501 catcaccagc ggcactgacg cgcagggtga ggtgacggtt cggctcgcca ggggcggacg
     1561 catcgtcaac gggcagggcg ccgacacgga tatcgtgatc gcgtcggcca aggcctatct
     1621 caacggcctc aacaagttgc acgcgaagtt cgagcgggcc cacccgcaag tctgagcgtc
     1681 caacgtatca tcaaagtgaa tcaggagtca ggtatgttgc aaggaagcat tcaagtcacc
     1741 agtttcgata tgagccgatt gttcgccatg atcgagcaga tgcgccagca tggcgttgtg
     1801 gagccggcca gcctggcgcg gctggaggat gagcttgccc gttgcgacga ggtggcggct
     1861 gaggcagtcg atgccgacgt cgtcaccatg aactcctgcg tcgaactgcg cgatctcgcc
     1921 agcgggcaga cgcacacgct gaccctggta ttcccgcagc aagctgacgc cgccgcgcat
     1981 cgtgtctcgg tgctggcgcc cttgggtacc gccatcctgg gttaccgcgt cggtgacacc
     2041 atcgattggc ccatgcccgc tggcgtcagg caataccaga tcctcgcgat ccgctaccag
     2101 ccagaggctg ccggcgattt ccatctctag gcgatgcctg ccggcgtcag ccggcggggt
     2161 cctgtttcgt cgatcgtcat gagtgaatcc acacatcccg cacgcctgcg gctgttggtg
     2221 ctgcttgccc tgttcgcgct ttatttcatc tggggttcga cctttctggc gatgaaattc
     2281 gccatcgaga gctttccgcc attcatgatg gcggcgatcc gcttctcgat cgccggcggc
     2341 ctgctctatg gtgtgctgcg tctgtacggc acgccggcgc cgacaggcag gcagtggctg
     2401 ggcgcatccg ccgtgggcat attgttgttg tcggtgggca atgcgggcgt ggcgatcgcc
     2461 gagcagaccg tatcttctgg ggtcgcggct ttgtcgatcg ccaccgttcc cttgtggatg
     2521 gccttgttct ccggcgtatg gggccattgg ccgagtcgac gcgaatggac cggtatcgtc
     2581 gtcggtacgg tcggcgtcgc cgtcctcagc accggcggca cgctgcaggc cagcccgatg
     2641 ggcactttcc tgctgttgct ggcggccgca agctgggcgt tcggttcggt ctggggcaag
     2701 cggctggcga tgccgcaagg ggccatggcg agtgccgcgc agatgctggc cggtggcgcc
     2761 atgctgttag tggcgagtac cgtcagtggc gagcactggc cggcggtggt gtcgatgaaa
     2821 tcggtctggg cgatcgtcta tctgatcatc ttcggctcat tcatcgccta cagcgcctac
     2881 ctctttctgc taaagactgt gcgtccagtg ctggcgacca gctacgcctt cgtcaatccc
     2941 gtggtcgcca tgctgctggg cgcctggctg gcggacgaac atatcggtcg cactgaatcc
     3001 gtggcgctgc tgatcatcat cgtcggcgtg ctgctggtgt tgccgttcaa gcgcaacgag
     3061 cccgcgaaga gccctagcgc cggaccttga gatagcgctg gtcgtcaaat gtgacgagcc
     3121 agtggggccg atagaccgcc atgagcgtca cggccatgcc ggtgagcagg gcttccgacc
     3181 aggccatgag catgtagaag ggcaggtact cgctgtgcag gtagctgctg gagtaagcgc
     3241 ctgcttccga caggatgaag cagctggcga atccgagcgc ggccatggcg acggcgccgg
     3301 cgaagaagcc atcgaccagc acatagatga agaaatggtt gggcagcttg cgatcagccc
     3361 agcggaacag gccgtggctg atgagcacgg gtagcagtgc catgagtacg aaattgatgc
     3421 cgtaactctc caggccggaa aggccgaacg ctgtcgccag catcagtacc atcgccaggg
     3481 cgatgagggc caggtaggga ccgaacatca gggtcagcac cgtcgcgccc agtacatgga
     3541 aattgaggcc gggcttgatg cccgcgcgta tgctccagag cacgcccagg ctgacgcaga
     3601 agccgaggaa cacattcagc atggtcgacg acaggatgcg ccagcgcgcc tgcctcgcgg
     3661 aatgcagcaa gacgagcagc gcaacggcgt tggcggccca cagcaggttg gtcggcagca
     3721 ggaggtcagg aaaattcacg agcgtatgac tgcgacattg aggtcaggtt ctgcagatca
     3781 taacgcatcg gttaaactcg taaagattca cagaaacgga gaagattttg cagcttgcct
     3841 tattcgatct agacaatacc ctgcttgcgg gcgacagtga ttttcagtgg gggcagttcc
     3901 tcatcgagga gggcctgctc gaccgagatg agcaccacga tcgcaatgtg caattctatg
     3961 aggattacaa ggccggcaag ctcgatatcg atgcctttct cgcgttccag ctcaagccgt
     4021 tgagccggca tagcaaggac gcgttgcacg ccctgcatcg caagtacatg gagcgcaagg
     4081 tgcgcccgat gatgacggac aaggcgcgcg cgctggtgga gaagcacaag gcgcaaggcg
     4141 atctcatgct catcatcacc gccaccaata gcttcgtcac caagccgatc gccaccgaat
     4201 tcggcatcga gcacctgatc ggcaccgatc cggaatgcgt ggatggccga tataccggcg
     4261 gggtcagcgg catcccgagt ttccagcagg gcaaggtcac gcgcctgcac cagtggctgg
     4321 aacaacgcgg ccagtacctg gaggatttcg agaagagctg gttctacagc gattcacaca
     4381 acgacctgcc gttactcaag ctcgtgaccg acccggtcgc agtcgatcct gatccggtat
     4441 tgcaggccca cgccgagcag cagggctggc ctatcatctc gcttcgctag gtcattttca
     4501 ggctggaggc tttgccgctg atggcctgga tctgtggttc aagaccggct cgcgcatcag
     4561 tgaaccggtc tttcatgatg acggctgggg tttaacggtg cagcaggatc tcgagcacga
     4621 atttgctccc cgcatacgcc aacagcaaca gcacgaagcc gatcagcgtc cagcggatcg
     4681 cagtgcggcc acgccagcca taggcgacac gccccagcag taagccgccg aagatcagcc
     4741 aggagaggat ggtgaacaag ttcttgtggt tgaacttgag cggctggtgg aacagggctt
     4801 cagagaacag catcccgctc agcaaggtca gcgtcagcag caggaagccc acgctgatga
     4861 cgcggaacag cagtgtctcc atggtcatca gcggcgggaa atccggcagc ttgagcaggg
     4921 tcggcttctg gtgcaggctg cgttccgcca ctgccatcag cagggcgtgc atggcggcga
     4981 aggtgaacag actgtaagcc agcatggcga tgaacaggtg ggcaaggaac atcggctggt
     5041 cggcataagg tagcaggtgc gtctccggac tgagcttctg cagcaatacg aagaatgcgg
     5101 ctggcggcaa cacgaacgcc tgcaggccat agaggcggtg cctgaaatcc gtgacccaat
     5161 agatcagcac cgtgagccag aagatggccg acaaggcatt gctgaaactg agattgaagc
     5221 cgccgtcgaa caggccgcgg tagagcacgt aaccatggag cgccagcccc gtatcgatgg
     5281 ccagcgcatg ccagcgtagt cgcgtcgtgc tggcggcggt cttgggattg aaccagatat
     5341 cggcggccac ccaaatatag atgacggcgg tcaagaggta ggggataaac tcgatcatgc
     5401 tttggcagtg acttgggtta aaatcgaatc ttatccatca ctataacatg cgccccagca
     5461 ggttttgcca ggcgcgcatg atcgaatcga caccatgtta gaaaatctga gcggaagatt
     5521 acagcaggtc atcaagacct tgcgcggtca ggcgcgcctg acggaagaga acatcgccga
     5581 cgccatgcgc gaggtgcgca tggccttgct ggaggccgac gtcgccttgc cggtggtcaa
     5641 ggaattcatc gcccacgtca aggagcgcgc gcaaggtcgc gaagtgctgc agagcctgac
     5701 gccgggccag gccgtcatcc aggtcgtgca tgaagaactg accaagctca tgggcgagca
     5761 gaacgccggc ctcgatctgg cgacgcaacc gcccgccatc gtgctcatgg ccggcttgca
     5821 gggttcgggc aagaccacga cctcggccaa gctggcaagg ctgctgaagg cggagaagaa
     5881 aaaagtcctg ctcgccagtg ccgacgtcta tcgcccggct gcgatcgagc agctgaagac
     5941 cctggcgcag agcctggaga tcgattgttt cgattccaac gccacgcaaa agcctgccga
     6001 tatcgccgct ggggcgatcg atttcgccaa gcgccacttt tacgatgtgc tgatcttcga
     6061 taccgccggt cgtctcgcga tcgacgaagc catgatggcc gagatcaagc agctgcatag
     6121 tttgctcaac ccgatcgaaa ccctgttcgt ggtcgacgcc atgcagggtc aggatgccgt
     6181 caacacggcc cgcgcctttg gcgagacgtt gccgctgacc ggtatcgtcc tgaccaagct
     6241 cgacggcgat gcgcgcggtg gtgcggcgct gtcggtgcgg catatcacgg gcaagccgat
     6301 caagttcatc ggtgtcagcg agaaggtcga cgggttggag cccttccatc cggagcgcat
     6361 ggcatcgcgt gtgctgggca tgggcgacgt gctatcgctg atcgagcaag cgcaaaagaa
     6421 cgtcgatctc gacgaagcga agaagctggc ggagaaggtc aagtccggca agaacttcga
     6481 tcttgatgac ttcaaggcgc agatgtcaca gatgcgcaag atgggcggcg tcggcgccct
     6541 catggacaag atgccggcgc agatggcagg catggcgggc cagatgagcg gcgaggccgg
     6601 cgacaaggcg atccgccgta tcgaaggcat catcaactcg atgacgccgc tggaacgccg
     6661 caagcccgat atcatcaagg ccacgcgcaa gcgtcgcatc gccgcgggcg ccggtgtgca
     6721 ggtgcaggag gtgaatcgcc tgctcaacca gttcgaggaa gtgcagaaga tgatgaagct
     6781 gttctccaag ggcggcatgg gcaagatgat gcgcgccatg cagggccgtt tccccggcat
     6841 gcgctgacag cccatcctgg catgcgcggc aggaattcct gattgacgcg atatcccctt
     6901 ttctggataa ttgcgccctt ccgtttggta tcgggttccc gatccagcgc agtttcagat
     6961 tccccggggc ggtagctcag tggtagagca gcggactttt aatccgttgg ccgtgggttc
     7021 gacccccacc cgccccacca gatctagcaa aaaggccagc atcgcgctgg cctttttgct
     7081 ttccgcaacc tggcgcggag cttac
//
LOCUS       DATMTW010000182         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_283806, whole genome shotgun sequence.
ACCESSION   DATMTW010000182 DATMTW010000000
VERSION     DATMTW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283806"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1533
                     /gene="purD"
                     /locus_tag="VN084_03405"
     CDS             265..1533
                     /gene="purD"
                     /locus_tag="VN084_03405"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWT28535.1"
                     /translation="MKVLVIGGGGREHALAWKLAQNKNVSRTYVAPGNAGTALDPDMV
                     NVPITTVPELVAFAENEQIALTIVGPEATLSQGVVDAFRAAGLRIFGPTQAAAQLESS
                     KDFAKAFMLRHGIPTAAYATFTDAAAAHDYVKQQGAPIVIKADGLAAGKGVVVAMSLD
                     EAHAAIDSMLSDNKLGSAGARVVIEEFLQGEEASFIVMVDGKHILPLATSQDHKRLLD
                     GDQGPNTGGMGAYSPAPVVTPEIHGKVMREIIKPTVAGMAKDGIPYTGFLYAGLMIGP
                     DGSVKTLEFNCRMGDPETQPIMLRLKSDLVALAEHAIDGTLDQAEAEWDRRTALGVVM
                     AAGHYPENPRTGDTITGLPKDLQDAYVFHAGTRMQGDAVVTSGGRVLCVTALGETVKY
                     AQARAYEIAEEIKFEGAQYRKDIGYRAVKG"
     gene            1675..2220
                     /locus_tag="VN084_03410"
     CDS             1675..2220
                     /locus_tag="VN084_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HWT28536.1"
                     /translation="MGNRGLAHYLRTGGVIAYPTESVFGLGCDPSNRTAVHRVLKIKG
                     RPQRKGLILIASGLGQLSPYIAPLTAEQQARLAQSSTLKPYTWVVPTAADCPRWLTGQ
                     HRSIAVRLTAHPLCRRLCSDSDMALVSTSANRSGGRPVKTTRDCLRRFGSQARIIRGR
                     TGGARRPSTIADLLTGRILRK"
     gene            2233..>2802
                     /locus_tag="VN084_03415"
     CDS             2233..>2802
                     /locus_tag="VN084_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HWT28537.1"
                     /translation="MNSQAVYEYLQNLQNRIVEALQLVDGKHFLHDSWQRPEGGGGTS
                     CMLEEGNVFERAGVGFSHVLGNKLPPAATIAHPEAAGRSWEAMGVSLVLHPRNPYIPT
                     VHMNVRFFVARAPKNGIAGQDVPSRPGGKEVNVQTAQSVRQTFADDANAVAGAQTDAD
                     IWWFGGGMDLTPYYGFEEDAVHFHRTCRDA"
BASE COUNT          562 a          925 c          821 g          494 t
ORIGIN      
        1 gcaggcgcat cctgtgtcat ccatccgggc ggcagcatgc gcgacgagga agtgatcgcc
       61 gcggccgacg aacatggcat cgcgatggtg ctgaccggca tccgccattt cagacactaa
      121 aaccatgatt aattcactgc gcggttcata cactttgtcg cgtgctcgct cactcctcgc
      181 ctaccctcat ggtatgcccc gtcattcgct gtgcggctcc ccgtctatga agccgctcgc
      241 tacaattact caaggtttta gaatatgaag gtacttgtca tcggcggtgg tggccgcgaa
      301 cacgctttag cctggaaact ggcccagaac aagaacgtca gccgcaccta tgtcgcgccc
      361 ggcaatgccg gcacggcgct cgatccggac atggtcaacg tgccgatcac gacggtgccg
      421 gaactggtcg ctttcgccga gaacgaacag atcgcgctga ccatcgtcgg ccccgaagcg
      481 acgctgtcgc aaggcgtggt cgatgccttc cgtgcagcgg gcctgcgcat cttcggcccg
      541 acccaggccg cagcgcagct cgaatcctcc aaggatttcg ccaaggcctt catgctgcgc
      601 cacggtatcc cgaccgcggc ttatgccacg ttcaccgatg ccgccgcggc ccatgattac
      661 gtcaagcaac agggcgcgcc catcgtcatc aaggcggatg gccttgccgc cggcaaaggc
      721 gtcgtggtcg ccatgtccct cgatgaagcg catgccgcca tcgattccat gctctctgat
      781 aacaagctcg gcagcgccgg cgcacgcgtg gtcattgaag agttcctgca aggtgaggaa
      841 gccagtttca tcgtcatggt cgacggcaag cacatcctgc cgctggctac gagccaggat
      901 cacaagcgcc tgctggacgg cgaccagggc ccgaacaccg gcggcatggg tgcctattcg
      961 cctgcgccgg tagtcacgcc agagatccat ggcaaggtca tgcgcgagat catcaagccg
     1021 accgttgctg gcatggccaa ggatggcatc ccctacaccg ggtttctcta tgccgggctc
     1081 atgatcggac ccgacggctc ggtcaagacg ctggaattca attgccgcat gggcgacccc
     1141 gaaacccagc ccatcatgct gcgcctcaaa tccgacctgg tcgcgctggc tgaacacgcc
     1201 atcgacggta cactggacca ggccgaggcc gagtgggatc gccgcacggc gctgggcgtg
     1261 gtcatggccg caggccatta tccggaaaac ccgcgcactg gcgacaccat caccggcttg
     1321 cccaaggacc tgcaagacgc ctatgtgttc catgccggca ccaggatgca gggcgacgcc
     1381 gtcgtcacca gcggcggtcg cgtactatgc gtcaccgcac tcggcgaaac agtgaagtat
     1441 gcgcaggcgc gcgcctacga gatcgccgaa gagatcaagt tcgagggtgc gcagtatcgc
     1501 aaggatatcg gctatcgcgc cgtgaaaggc tgaagaccat gccgctcaac ggctgcgcga
     1561 atgaatcacg atgtcctgcg gtggactggc gcatgccctt cgctgctgac gccttgtact
     1621 tcgatacgtt cgctcgttta tcagcctatt cttaagcatt gctcgagact cgctttgggt
     1681 aaccgtggat tagctcatta tttacgcacc ggcggcgtca tcgcctatcc gaccgaatcc
     1741 gtcttcggac taggctgcga tccgtccaat cgcaccgcag tgcatcgcgt gctcaagatc
     1801 aaggggcggc cgcaacgcaa gggcctgatc ctcatcgcca gcggtctcgg ccagctcagt
     1861 ccctatatcg cgccactcac tgccgagcaa caagccagac tcgcccagag cagcactctc
     1921 aagccttaca cctgggtggt gccgaccgcg gcagactgtc cacgctggct caccggccag
     1981 catcgttcca tcgccgtaag gctgacggcg cacccgctat gccgccgact ctgcagcgac
     2041 agcgacatgg cgctggtctc caccagcgcc aaccgtagcg gtggccgccc ggtaaagacc
     2101 acgcgcgact gtctgcgccg tttcggcagc caggcacgga tcatccgtgg ccgcacaggc
     2161 ggtgcgcgcc gcccctcgac catcgccgac ctgctgactg gtagaatcct caggaaataa
     2221 cccggaaccc gcatgaattc tcaagccgta tacgaatacc tgcagaacct gcagaaccgc
     2281 atcgtcgaag ccctgcagct ggtcgacggc aaacacttcc tgcacgacag ctggcagcgc
     2341 ccggaaggcg gcggcggaac atcgtgcatg ctggaggaag gtaatgtgtt cgagcgcgca
     2401 ggcgtcggtt tctcgcatgt gctgggtaac aaactgcctc cggccgcgac catcgcccac
     2461 ccggaagccg ccggtcgcag ctgggaagcc atgggcgtct cgctggtgct gcatccgcgc
     2521 aacccctata tccctacggt gcacatgaac gtacgctttt tcgtggcacg cgcacccaag
     2581 aacgggatag ccgggcagga tgtgccgtcg agaccaggcg gcaaggaagt aaacgtgcag
     2641 acagcacaat cagtgcggca aacgtttgct gacgacgcca acgccgtcgc aggcgcacag
     2701 acagacgcgg atatctggtg gtttggtggc ggcatggacc taacccccta ctacggcttc
     2761 gaagaagacg ccgtgcactt ccatcgcact tgccgggatg cg
//
LOCUS       DATMTW010000183         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_317629, whole genome shotgun sequence.
ACCESSION   DATMTW010000183 DATMTW010000000
VERSION     DATMTW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317629"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VN084_03420"
     CDS             <1..320
                     /locus_tag="VN084_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HWT28538.1"
                     /translation="LKVDVQEDEHRYTVHAEIPGVKKEDINVTIDGNHIAISAEVRRE
                     KEVKDGARMLRSERHYGKVARSFVLESEVDEAKADASYKDGVLELTLPKKSVTAAKRL
                     TVN"
     gene            complement(421..2094)
                     /locus_tag="VN084_03425"
     CDS             complement(421..2094)
                     /locus_tag="VN084_03425"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWT28539.1"
                     /translation="MPSDIEIAQQAQLKKISEIASALGLAEDSVEHYGQYKAKIDIRR
                     NPQLAERSDGKLILVTAISPTPAGEGKTTTTVGLGDALNRIGKKAVVCLREPSMGPVF
                     GMKGGAAGGGHAQVVPMEDINLHFTGDFAAIALAHNLLSALIDNHIHQGNALGIDPRQ
                     VTWKRVMDMNDRALRQIVVGLGGKVNGQTREDGFDIVVASEVMAIFCLAESLKDLKQR
                     LGNIVIGYSYLGEAVTARDLKAHGAMAVLLKDAIKPNLVQTLEHTPAFIHGGPFANIA
                     HGCNSVIATKLGLKLGDYVVTEAGFGADLGAEKFIDIKCRKSGLKPAACVVVATIRAL
                     KYHGGVEVADLGREDLLALERGLDNLHKHVENVTQHYGLACVVALNRFSNDTDAELTL
                     VREHLAKQGVHVVLAEHWARGGAGAEDLAREVVRLVEGGHSQMTFVYDDTDNLWEKMR
                     KVATKVYAAADIFAPRRLPVQIEQLQAEGYGHYPICVAKTQYSFSTDAMARGAAKGHI
                     INVRAVRLCAGAEFIVMICDDIMTMPGLPKEPCANRIDIDDDGVVSGLY"
     gene            2290..3174
                     /gene="argB"
                     /locus_tag="VN084_03430"
     CDS             2290..3174
                     /gene="argB"
                     /locus_tag="VN084_03430"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWT28540.1"
                     /translation="MLTDTSAAQKAKTLAEALPYIKRFFDKTIVIKYGGNAMTDPKLK
                     ECFASDVVLLKLVGMNPVIVHGGGPQINELLDRLGKKGEFIQGMRVTDEETMDIVEMV
                     LGGQVNKEIVHLINRHGGKAVGLTGQDGNFIHATKLLMANKELPGEFIDIGQVGDVTA
                     IDPTLIEFLDTGDFIPVIAPIGVGPDGETYNINADVVAGKLAEILGAEKLVLLTNTPG
                     VLDKEGTLLTGLTPKQIDDMVADGTLSGGMLPKISSALDAARSGVKSVHIIDGRVEHA
                     LLLEVLTDEGVGTLIKAK"
     gene            3327..>3687
                     /locus_tag="VN084_03435"
     CDS             3327..>3687
                     /locus_tag="VN084_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HWT28541.1"
                     /translation="MSRVWVFDLDDTLHDASAHIFPVMNQAMTQYIMDHLDLNEDDAH
                     ALRRHYWRIYGATMKGLTRHHDIDPRHFLHITHQFPELAQMVVRSQRLRHCLQRLSGR
                     KVVFTNAPMGYALRVLKL"
BASE COUNT          754 a         1146 c         1075 g          712 t
ORIGIN      
        1 aactcaaggt ggacgtgcag gaagacgaac accgttacac cgtgcatgcc gagatccccg
       61 gcgtgaagaa ggaagacatc aatgtcacca tcgacggcaa ccacatcgcc atcagcgctg
      121 aggtccgccg cgagaaggaa gtgaaggacg gcgcccgcat gctgcgcagc gaacgccatt
      181 acggcaaggt agcgcgcagc ttcgtgctgg agagcgaagt cgacgaagcc aaggccgacg
      241 ccagctacaa ggacggtgtg ctggaactga cgctgccgaa gaaatcggtg accgcagcca
      301 agcgcctcac cgtcaactga tcctcaacga ttgtgtttct cctcgactcg cggccttcgg
      361 gccgcatttt tttgcctggc gcccgccgca caccaccatc tgccgacatc gcaacctcaa
      421 ctagtacaag ccggagacca cgccgtcatc gtcgatatcg atgcgattgg cgcaaggctc
      481 cttgggcaag cctggcatgg tcatgatgtc gtcgcagatc atgacgatga actccgctcc
      541 tgcgcatagc cgtaccgcgc gcacgttgat gatatggccc ttggcggcgc ctcgcgccat
      601 ggcgtccgtg gagaacgaat actgagtctt ggcgacacag atcgggtaat ggccgtagcc
      661 ttctgcctgt aattgctcga tctgcaccgg caggcggcgt ggcgcaaaga tgtcggccgc
      721 ggcatagacc ttggtggcga ccttgcgcat cttctcccac agattatccg tatcgtcgta
      781 aacgaacgtc atctgactgt ggccaccctc gaccagacgc acgacttcac gcgccagatc
      841 ttccgcgccg gcccctcccc ttgcccagtg ctccgccagc acgacatgta cgccttgctt
      901 cgccaggtgt tcgcgcacca gcgtcagctc ggcatccgta tcgttactga agcggttgag
      961 ggcgaccacg caagcaagac cgtaatgctg agtgacgttc tcgacatgct tgtgcaggtt
     1021 gtccagcccg cgctccagcg ccaataagtc ctccctgccg agatcggcca cctcgacacc
     1081 gccgtggtac ttgagcgcgc gaatggtggc gaccaccacg caagccgctg gcttcaggcc
     1141 ggacttgcgg catttgatgt cgatgaattt ctccgcgccg agatcggcac cgaaaccggc
     1201 ctcggtgacc acgtaatcgc cgagcttgag gccgagcttg gtggcgatca ccgagttgca
     1261 gccatgcgcg atattggcga acgggccgcc atggatgaat gccggcgtgt gttccagtgt
     1321 ctgcaccaga ttgggcttga tggcgtcctt cagcagcacg gccatggcgc cgtgcgcctt
     1381 gagatcacgc gccgtgaccg cttcgccgag gtaactgtag ccgatgacga tattgcccaa
     1441 tctctgtttc aggtccttga gcgattcggc caggcagaag attgccatga cctcggaagc
     1501 gacgacgata tcgaaaccgt cttctcgcgt ctggccgttg accttgccgc ccagaccgac
     1561 gacgatctgg cgcagggcgc ggtcgttcat gtccatgacg cgtttccagg tgacctggcg
     1621 cgggtcgatg ccaagcgcgt tgccctggtg gatatgattg tcgatgagtg cggacaagag
     1681 gttgtgcgcc agcgcgatgg ccgcgaaatc ccctgtgaag tgcaggttga tgtcctccat
     1741 gggcaccacc tgcgcatgcc cgcccccggc cgcaccgcct ttcatgccga acaccggccc
     1801 catggagggt tcgcgcaggc agacgaccgc cttcttgccg atgcggttca gcgcgtcacc
     1861 gagacctacg gttgtggtgg tcttgccctc tccagccggc gttgggctga tggcggtcac
     1921 gaggatgagc ttgccgtcac tgcgctcagc gagctgcgga ttgcgccgga tatcgatctt
     1981 ggccttgtat tgcccgtaat gctcgactga atcttccgcc aagccgaggg cgctggcgat
     2041 ctcgctgatc tttttgagct gggcttgttg ggcgatctcg atatcactcg gcatggacac
     2101 ctctcaaatt aaaaccgcgt ctgcatgatt gcctacctga cggaccgacc ctcaccacgc
     2161 ctgcgcgcag atgtgcgatt gctgatgaag ccggcatcct gacagtctga aagttagcat
     2221 atttggacac ggctttaggg gatttttcaa tgtaaaatac tcgcagtcat tcacttgccg
     2281 agagagtcaa tgctcaccga caccagcgcc gcccagaaag ccaagaccct tgccgaagcc
     2341 ctgccttaca tcaagcgctt cttcgacaag accatcgtca tcaagtacgg cggcaacgcc
     2401 atgaccgacc ccaagctcaa ggaatgcttc gccagcgatg tggtgctgct caagctggtc
     2461 ggcatgaatc cggtgatcgt ccacggtggc ggcccacaga tcaatgaatt gctggacagg
     2521 ctcggcaaga agggcgagtt catccagggc atgcgcgtca ccgacgagga gaccatggac
     2581 atcgtcgaga tggtgctcgg cggccaagtc aacaaggaga tcgtgcatct catcaaccgc
     2641 cacggcggca aggccgtcgg cctcaccggg caggacggta acttcatcca cgcaaccaag
     2701 ttgctgatgg cgaacaagga gctgccaggc gaattcatcg acatcggtca ggtcggcgac
     2761 gtcaccgcca tcgatccgac cctgatcgag ttcctcgaca cgggcgattt catcccggtc
     2821 attgccccga tcggcgtcgg tccggatggt gagacctaca acatcaatgc cgacgtcgtc
     2881 gctggcaagc tggccgagat cctcggcgcc gagaaactcg tcctgctcac caacacgccg
     2941 ggcgtgctgg ataaagaagg caccttgctc accggcctca cgcccaagca gatcgacgac
     3001 atggtcgccg acggcaccct ctccggcggc atgctgccca agatcagttc ggcgctcgat
     3061 gcggcgcgca gcggcgtgaa gtcggtgcac atcatcgacg gacgtgtcga gcatgcattg
     3121 ctgctggaag tcctcacgga cgaaggggtg ggcaccctga tcaaggccaa ataattccgt
     3181 atttcattgg cttgctgaga cggcttcaga gatgtttttc cgggatgaag cgcaaggcgc
     3241 cgcatagcga atgacgagac atatcgaacg gatagacgag gaatgagcgc gcacgcaacg
     3301 ccgcgattcg cccgcaaaat ggatctatga gccgcgtctg ggtttttgac ctggacgata
     3361 ccttgcacga cgcgtccgcc cacatcttcc cggtgatgaa ccaggccatg acgcagtaca
     3421 tcatggatca tctcgatttg aacgaggatg acgcgcatgc cttgcggcgg cattactggc
     3481 gcatctatgg cgcgaccatg aaagggctca cgcgccatca cgacatcgac ccccggcact
     3541 tcctgcatat cacccaccag ttccccgagc tggcgcagat ggtcgtgcgc agccaaaggc
     3601 tccggcactg cctccagcgc ctttcagggc gtaaggtagt attcactaac gcgcccatgg
     3661 gttatgcttt acgggtgctg aaactga
//
LOCUS       DATMTW010000184         8683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_552864, whole genome shotgun sequence.
ACCESSION   DATMTW010000184 DATMTW010000000
VERSION     DATMTW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552864"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VN084_03440"
     CDS             complement(<1..722)
                     /locus_tag="VN084_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HWT28542.1"
                     /translation="MRDDVHVADHSPSDAGFVAKVEQACRDACAAIAPAWPLDRAIAV
                     NPHWSRIGMPVRQVAARMGVLGSIDVFPPRDRQLDAWQSGRIQPGDLQQALDQLPEAR
                     AAKLTAADCVEALHSGSHLQPLPLLIDVLDNDPQRHSRLSWRQAITYQVSQTCAAYFD
                     EHQADWHPERNQGLYAFWRETLRHDHGIGILMGLPHIGHAIDALPTDARDAERWVVNR
                     LGLPETVWAEYLESVLLTVNGWA"
     gene            complement(777..2333)
                     /locus_tag="VN084_03445"
     CDS             complement(777..2333)
                     /locus_tag="VN084_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWT28543.1"
                     /translation="MPTFELIRAFSPLAMAAIMMLVAMLTRLDRIPTPTLWLTFRRAS
                     FLAMVIAGALVFISPIPMVMPSAFQASTLSLTIAFLVQLLGTIISAFSSRYLEGEYGQ
                     RGYIAAFAIVLGAVHLLLLADHWVVLILAWALVGIALQRLLCFYPERAFARLAAHKKR
                     IADRLADALLISAASLAWMEVGSGSLSALWIHVAQHGLSVAMQFSALCMALAVVLRTA
                     LLPVHGWLIQVMEAPTPVSALLHAGVVNLGGFVLIRFAPLLEHATAARTLLLVFGLST
                     AVLAGMVMLTRITIKVRLAWSTVAQMGFMLLECALGLYTFAALHLIGHSLYKAHTFLS
                     ASSVVRQTRQAMLLTVEPLLAMSLLAAPLLSTGLVLGIQTAIGQHDWPWWWSGILGLA
                     WAPLLWLREGDRLRGMTQALFGLLVVAGLTVAALLGHALPLGIHDSPRHALGLGALAG
                     MSVLYLCLMTLQLKPRLLDNWRRRSYAGFYVDEVYTRLALRYWPTRWMPERATHAAST
                     TTDRALFDAH"
     gene            2431..3348
                     /locus_tag="VN084_03450"
     CDS             2431..3348
                     /locus_tag="VN084_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWT28544.1"
                     /translation="MRLEQLNFHHLFYFWRVAKLGHLTRAAHELHTSQSAVSAQIRQL
                     EERLEETLFTRQGRRLLLTHTGQLVFAYAENIFGLGQEMLGRLQGRSAGVTRLRVGSV
                     ATLSRNYQENWIRPLLADPSIVLTLESGLMEGLVSRLLQHQLDVVLANETVPTDPNRP
                     LHCRFLGSQSISLVGPPGRWRSKSLRIPEDLHDVDVVLPGPRHAIRAQFDALCASAGV
                     TPRLRAEVDDMAMLRLIARDSGWLTVLPEVVVQDELRTGTLVIVGQSSELQERFYAIT
                     TPQRHYIEPLEALLSGEVFTGGPDIPPAS"
     gene            3440..4681
                     /locus_tag="VN084_03455"
     CDS             3440..4681
                     /locus_tag="VN084_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWT28545.1"
                     /translation="MLEHLLPVTTWLEDRVVLQMLIYGLGLVGLAWFVNTFIKVLLLK
                     LAFGLLQAMPLEHRGSGRKLIRRLANILPAFVISVCIALIPDIHPVFVAVVQNVANAF
                     IVLVIALAISDALDVANSFYQMRKDAHLHPIKGYLQIAKILVFALASILIVATLIDRS
                     PLILLSGIGAMAAVLMLIFQDTLLSLVASIQISSNDMVRVGDWIEMPQLNADGDVIDV
                     ALHTVKVQNWDKTITTIPTKRLISESFKNWRGMQESGGRRIKRSLHLDQNSIGFLDAE
                     SLQRMRTFDVLQDYLESKLTEIDGWNRRVTDKGLAPVNSRRITNIGIFRAYIEQYLRH
                     HPRIQQDMTLLVRQLAPGEHGLPIEIYCFTNTVNWNAYEAIQSDIFDHLLAILPQFGL
                     RVFQNPTGMDLTALAHSRMRA"
     gene            complement(4693..5862)
                     /locus_tag="VN084_03460"
     CDS             complement(4693..5862)
                     /locus_tag="VN084_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HWT28546.1"
                     /translation="MKLTAWQRPLFLLILLQPWLAMAAEPWLAAPRDTTLGPGKVVTL
                     DIVKPAEAAWPAQLRLTLDGVGSEETLVLTAIDAGAADGDRRAYQARPRSRFSGVVKA
                     RLVDVPANAFLLQASVDDDIGPLEVTHEEDHAQPAPRNASPTIVIAKPGDEPGLSANE
                     PAYFVLGSDSRHGADARFQLSFKYRLFDPKSALSEFSPLFSNLYFTYTQTSLWDIGDD
                     SSPFRDTSYRPGLYYGWAGNGTQWLPDDWRLGLEHESNGQSGADSRSINIAYVRPIWH
                     AEMDHGRRLTFMPKIYDYLSKSDNEDIQRYRGYADWMVRYGREDGLIVNGMYRQGTAG
                     YASGQVDLSYPLSARIFARTGTFVHLQLFSGYGETLLDYNRERDTQLRIGLSIAR"
     gene            complement(5948..7756)
                     /locus_tag="VN084_03465"
     CDS             complement(5948..7756)
                     /locus_tag="VN084_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWT28547.1"
                     /translation="MTKSRSFLALASLCLILAGISACTQIDKKDPTTSHIAQAAKGEW
                     PSFGRDYANQRFSPLTQVNRQNVGKLGLAWHYKSGVKASFQTTPIVVDGVMYVSLPYN
                     DVVALDARTGKQLWRYVHQRRKDWKLCCGPANRGVAVGYGKVFMGTVDARLIALNAKT
                     GKVEWDIDAVDAAVATEGQESLNSNDPNRNAKTMGGTGVGIAMAPVVYNGKVIIGITG
                     VGYGLHIDNPRDDAPLGAVIGVTGRYGRPGFLAAFDVQSGKRVWQFDTIPTQGWEGKF
                     VETTTDGVPLNRDIATEKARLGDYPDAARFGGGSAWSTPAIDPTHGLLFFGTGNPSPQ
                     MNDVARPGDNLYTVSLVALDIDSGKIRWHYQQVPHDLWGYDLASPPVLFDLMRDGKRI
                     PVVGQAGKTGWFYVHDRMTGELLLKSEAFVPQKNLFVKATFEGTRLYPGILGGANWSP
                     VALDQANQRVFVAAIHAPIMYKLHETPGKDGKPAIRYASSEPVEEARWGLLSAIDLTT
                     GKIAWQQKTEQPLVGGVLATAGGLVFTGEGNGNFNAYDQKTGERLWHDKAEAGVNAPP
                     ITYEIDGVQYVAVAAGGNSIFGYKQGDNILVYALSK"
     gene            7930..8394
                     /locus_tag="VN084_03470"
     CDS             7930..8394
                     /locus_tag="VN084_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWT28548.1"
                     /translation="MSLYANLKLEHQLCFALYAATNAITRTYRGKLTQVGLTYPQYLV
                     MMVLWAQDGISVKEIAQRLQLDSATITPLLKRLESIGYVLRSRSSDDERVVNVRLTED
                     GHAIQERVAKLQSEVECQTRLDQAEFFALRAQLHRLAETLNQPPPAEDQQVA"
     gene            8538..>8683
                     /locus_tag="VN084_03475"
     CDS             8538..>8683
                     /locus_tag="VN084_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWT28549.1"
                     /translation="MKSNQTATKDAFPIRTVAALTGINPITLRTWERRYGLIAPIRTP
                     KGHRL"
BASE COUNT         1782 a         2619 c         2589 g         1693 t
ORIGIN      
        1 gcccagccgt tgacggtgag caggacagac tccaggtatt ccgcccaaac ggtttcgggc
       61 agacccagcc ggttcaccac ccaccgctct gcatcgcgcg catcggtggg caaggcgtcg
      121 atggcatgac cgatgtgcgg caagcccatg aggatgccga tgccatggtc atgccgcaag
      181 gtctcgcgcc agaaggcata gaggccctgg ttgcgctccg ggtgccaatc ggcctgatgt
      241 tcatcgaagt aggctgcgca ggtctggctg acctgataag tgatcgcctg ccgccacgag
      301 agcctgctgt ggcgttgggg gtcgttgtcg agcacatcga tgagtagcgg cagcggctgc
      361 aggtggctgc ctgaatgcag ggcttcgaca caatcggcgg cagtcagctt cgcagccctt
      421 gcctctggca actgatccag ggcttgctgc agatcgcccg gctggatgcg cccgctttgc
      481 caggcatcga gctgccgatc gcgtggcgga aagacgtcga tactacccag cactcccatg
      541 cgcgcggcga cctgccgcac aggcatgccg atacgcgacc agtgcgggtt gaccgcgatc
      601 gcgcggtcaa gtggccaggc tggcgcgatt gcagcacagg catcgcgaca ggcttgttct
      661 accttggcca cgaatcctgc gtcggatggt gaatgatcgg cgacatgcac atcgtcgcgc
      721 atgtcgaaac tagccgtaga tgcttccatt agatcgtttt ccttgtcgtt tttggatcaa
      781 tgggcgtcaa acagtgcccg atcggtcgtc gttgaagctg cgtgtgtcgc gcgttccggc
      841 atccaacggg tgggccagta gcgcagcgcg agtcgtgtat acacctcgtc cacatagaaa
      901 ccggcgtagc tcctgcgccg ccagttgtcg agcagtcgcg gcttgagctg cagggtcata
      961 aggcacaggt acaagactga catgccagcc aacgcaccca agccgagggc atgacgcggc
     1021 gagtcgtgga tgccgagggg tagcgcgtgt ccgagtagtg cggcgacggt gaggcctgcg
     1081 acgaccagca atccgaacag ggcctgcgtc atgccgcgca aacgatcgcc ttcgcggagc
     1141 cacaacaacg gcgcccatgc cagcccgaga atgccgctcc accaccatgg ccagtcgtgt
     1201 tgcccgatgg ccgtctggat gcctagcacg aggccggtag acaacagcgg tgcggcgagc
     1261 aggctcatcg ccagcagcgg ctcgacggtg agtagcatgg cctgccgcgt ctgccggacc
     1321 accgatgaag ccgaaaggaa ggtatgggcc ttgtagaggg agtgtccgat caggtgtagc
     1381 gcggcgaagg tgtagagccc cagcgcgcac tcgagcagca tgaagcccat ctgcgccacc
     1441 gtggaccagg caagccggac tttgatcgtg atgcgcgtca gcatcaccat gcctgccagc
     1501 accgcggtcg acaggccaaa caccagcagc agcgtgcgtg ccgcggtggc gtgttcgagc
     1561 agtggcgcga aacggatcag cacaaagccg cccagattga cgacccccgc gtgcagcaag
     1621 gctgacaccg gggtcggcgc ctccatgacc tggatcaacc agccatgcac aggcagcaac
     1681 gccgttcgca acacgaccgc caaggccatg cacagcgcgc tgaattgcat cgctaccgag
     1741 agtccgtgct gcgccacatg tatccagagc gcagacaatg acccgctgcc aacttccatc
     1801 catgccagcg acgccgcgct gatcagcagt gcgtcagcca ggcggtcggc gatccgcttc
     1861 ttgtgggctg cgagcctggc gaacgcgcgc tccggataga aacagagcag tctttgcagg
     1921 gcgataccga ccaaggccca ggccaggatc aggaccaccc agtgatcagc cagcaacagc
     1981 aaatgcacgg cccccaatac gattgcgaat gcggcgatat agccgcgctg accgtactcg
     2041 ccctcaagat aacgactgga aaaggcgctg atgatggtgc ctagcaactg cacgagaaaa
     2101 gcgatcgtca ggctgagcgt cgaagcctgg aatgccgatg gcatgaccat ggggatgggc
     2161 gaaatgaaga ccagtgcgcc ggcgatcacc atcgccagaa aggatgcgcg tcgaaaagta
     2221 agccacaaag tcggcgtcgg gatccggtcc agacgggtca gcatcgcgac gagcatcatg
     2281 atggcggcca tggccaaggg gctgaatgcg cgaatgagtt caaatgtagg catcgagaag
     2341 tccgggtgaa aaaattgcgt gcatgatgct tgccaattta tgttctgtaa attacattta
     2401 tccgaacttt ttgatactga aaaatgaacg atgcggctag agcaactcaa ctttcatcac
     2461 ctgttctatt tctggcgcgt ggccaagctt ggccacctga cccgcgccgc gcacgagctc
     2521 catacctcgc aatccgcggt gtcggcgcag atccggcagc ttgaggagcg cctggaagag
     2581 accttgttca cacggcaggg ccgacggctg ctactgacgc ataccgggca gctggtcttc
     2641 gcctatgccg agaatatctt tggcctgggt caggagatgc tggggcggct gcagggtcgt
     2701 tccgccgggg tcacacgttt gcgggtaggc agcgtggcca ccttgtcacg taattatcag
     2761 gagaactgga tccggccttt gctggcggat ccttcgatcg tccttaccct ggaatcgggg
     2821 ctcatggagg gattggtttc ccggctgctg caacatcaac tggatgtggt gctggccaac
     2881 gagacggttc cgacagaccc taaccgcccc ctgcactgtc gcttcctcgg cagtcaatcc
     2941 atctcgctgg tcgggccgcc cggtcgctgg cgatccaaaa gcttgcgcat cccggaagac
     3001 ctgcatgatg tggatgtcgt gttgcccggg ccgagacacg cgatccgcgc gcaattcgat
     3061 gcgctgtgcg cgtctgccgg cgtcacgccg cgcttgcggg cagaggtcga cgacatggcg
     3121 atgctgcgcc tgatcgcacg cgatagcggc tggctgaccg tattgccgga agtggtcgtg
     3181 caggatgagt tgaggacggg cacgctcgtg atcgtcgggc aatcgagtga gctgcaggag
     3241 cgcttctacg ccatcaccac gcctcaacgg cactacatcg agccgcttga agcgcttttg
     3301 tccggcgaag ttttcaccgg cggccccgac ataccgcctg ccagctgatc accctccttc
     3361 aggatgacgc gcgcggaacc atcgctgctc cgcggactcc tgttatatat ccattcgttt
     3421 ttataggaac ccattaccca tgcttgaaca tctattgccc gtcaccacct ggctcgaaga
     3481 tcgtgtcgta ttgcagatgc tgatctacgg actaggcctt gtcggtctcg catggttcgt
     3541 caacaccttc atcaaggtgc ttttgctgaa actggcgttc ggcctgctgc aagcgatgcc
     3601 gctcgagcac aggggctccg gccgcaagct gatccgtcgc ctcgccaata tcctgccggc
     3661 tttcgtcatt tcggtttgta tcgcactgat ccctgacatc catccggtat tcgtcgctgt
     3721 ggtacagaac gtcgccaacg ccttcatcgt actggtgatc gcgctggcga tcagtgacgc
     3781 gctcgatgtg gcgaacagct tctaccagat gcgcaaggat gcccacctgc acccgatcaa
     3841 gggctatctg cagatcgcca agatcctggt cttcgcgctg gccagcatcc tcatcgtcgc
     3901 cacgttgatc gatcgatcgc cgctgatcct gttgtcaggg atcggtgcga tggctgcggt
     3961 gctgatgctg atcttccagg acaccctgtt atcgctggtg gccagcatcc agatcagttc
     4021 gaacgatatg gtgcgggtcg gcgactggat cgagatgccg cagctcaatg ccgatggcga
     4081 cgtcatcgat gtggcactgc acacggtcaa ggtgcagaac tgggacaaga ccatcaccac
     4141 catccccacc aagcgcctga tctcggaatc gttcaagaac tggcgcggca tgcaggaatc
     4201 cggcggtcgg cgcatcaagc gcagcctgca tctcgaccag aacagcattg gttttctgga
     4261 cgcggaatcc ttgcagcgga tgcgcacatt cgatgtcttg caggattatc tggaatcgaa
     4321 gctgacagag atcgacggct ggaaccgccg cgtgaccgac aaaggcctgg cgccggtgaa
     4381 tagccgtcgc atcacgaata tcggcatatt ccgcgcctat atcgagcagt atctgcggca
     4441 tcacccacgt atccagcaag acatgacgct actggtgcgc cagctggcgc cgggtgaaca
     4501 tggcttgccg atcgagatct actgtttcac caataccgtc aactggaacg cgtacgaagc
     4561 gatccagtcc gatatcttcg accatttgct cgcgatcctg ccgcaattcg gtctgcgcgt
     4621 attccagaac ccgacaggca tggacctgac cgccctcgcc cacagccgta tgcgcgctta
     4681 gggcgagcac ggctaacggg cgatggacaa gccgattctg agctgggtat cgcgctcgcg
     4741 gttgtagtcg agcaaggtct cgccatagcc ggaaaatagc tgcagatgca cgaaggtgcc
     4801 ggtgcgcgcg aagatgcgcg cactcagcgg gtacgacaga tcgacctggc cgctggcata
     4861 gcctgccgtg ccctgccgat acatgccgtt gacgatcagg ccatcctctc gtccgtagcg
     4921 gaccatccag tcggcatacc cccggtaacg ctggatatcc tcgttatcgc tcttactcag
     4981 gtaatcatag atcttgggca tgaaggtcag gcgtcggcca tgatccatct cggcatgcca
     5041 gatgggccgc acataggcaa tgttgatcga acgcgaatcc gcgccggatt gcccattgga
     5101 ctcatgctcc agcccgaggc gccagtcatc gggcagccac tgcgtgccat tgccggccca
     5161 gccataatag agcccagggc ggtagctggt atcgcgaaat gggctggaat cgtcaccgat
     5221 gtcccatagc gaagtctgcg tgtaggtgaa atagagattg ctgaacagcg gcgagaattc
     5281 ggaaagcgcg ctcttcgggt cgaacagccg gtatttgaag ctcagctgga accgggcgtc
     5341 ggcaccatgc ctggaatccg aaccgagcac gaaataagcc ggctcattgg cagacaagcc
     5401 gggctcgtca ccaggcttgg cgatgacgat ggtcggcgat gcattgcgcg gcgctggctg
     5461 ggcatgatct tcttcatgag tgacttccag cgggccgata tcgtcatcca ccgaggcctg
     5521 caagaggaag gcattggcgg gcacgtcgac cagccttgcc ttgaccacac cgctgaagcg
     5581 ggatcgcggg cgcgcctgat acgcgcgccg gtcgccgtcg gccgcgccag cgtcgatcgc
     5641 ggtaaggacc agcgtctcct cgctgccaac gccatccagg gtcaaccgta gttgcgcagg
     5701 ccatgcagct tccgcaggtt tgacgatgtc gagcgtgacg accttgccgg gaccgagcgt
     5761 agtgtccctg ggggctgcca gccaaggttc tgcggccatc gcgagccatg gctgcaacag
     5821 gatcagcagg aacaatggcc gctgccatgc agtgagcttc atgttgatac cttcaaaaaa
     5881 aaatcctctg ccggccgtcc ctgcagagga tatctgtgaa tgcgcctatc agacgcgatg
     5941 agggatgcta cttggacaaa gcatatacca agatgttgtc gccttgttta tatccgaaga
     6001 tggaattgcc ccctgcagcg accgcgacgt attgcacacc atcgatctcg taggtgatgg
     6061 gcggcgcgtt gacgccggct tccgccttgt catgccacag acgttcgccg gtcttctggt
     6121 cgtaagcgtt gaagttgccg ttgccctctc ccgtgaacac gaggccacct gccgtcgcaa
     6181 gcacgccgcc gacgagcggt tgctcggtct tctgttgcca ggcgatcttg cctgtcgtca
     6241 ggtcgatggc ggagagcagg ccccagcggg cttcttccac aggctcggac gaagcgtagc
     6301 ggatcgccgg tttaccgtcc ttgcccggcg tttcgtgcaa cttgtacatg atcggggcat
     6361 ggatcgcggc gacgaaaacg cgttgattcg cctgatcgag cgcgaccggt gaccagttgg
     6421 cgccacccag gatgccagga tacaagcgcg tcccctcgaa tgtggctttg acgaacaggt
     6481 tcttctgcgg cacgaacgct tcagacttca gcagcagctc gcctgtcatc cggtcatgca
     6541 catagaacca gcctgtcttg ccggcctgac cgaccaccgg gatacgcttg ccgtcgcgca
     6601 tcaggtcgaa cagcactggc gggctggcga ggtcatagcc ccagaggtca tgcggtacct
     6661 gctggtaatg ccagcggatc ttgccgctat cgatgtcgag cgcgaccagc gataccgtgt
     6721 aaagattgtc gccagggcgt gccacatcat tcatctgcgg cgaagggtta cccgtgccga
     6781 agaacagcag cccatgcgtc gggtcgatgg ctggcgtgct ccaggcagaa ccgccgccaa
     6841 aacgcgccgc atccggataa tcgcccagac gcgccttttc tgtggcgata tcgcggttga
     6901 gaggcacccc gtcggtggtg gtttcaacga acttgccttc ccagccttgg gtggggatcg
     6961 tatcgaactg ccatacgcgt ttgcccgact gcacgtcgaa agccgccagg aagccaggac
     7021 ggccataacg gccggtgacc ccgatcacgg cgccgagcgg cgcatcgtcg cgtggattgt
     7081 cgatatgcaa accatagccg acgcccgtga taccgatgat gaccttgccg ttatagacca
     7141 caggcgccat ggcgataccg acgccagtgc cgcccatggt cttggcattc cggttggggt
     7201 cgttgctgtt gagcgactct tgcccttcgg tggcgactgc ggcgtcaacc gcgtcgatat
     7261 cccattccac cttgccagtc ttggcgttga gcgcgatcaa gcgcgcatcg accgtaccca
     7321 tgaaaacctt gccgtaaccg acagccaccc cacggttggc cggtccgcag cacagtttcc
     7381 agtccttgcg gcgctgatgc acatagcgcc agagctgctt gcctgtgcgg gcatccaggg
     7441 ccacgacgtc gttataaggc agcgagacgt acatcacacc gtcaacaacg atcggcgtcg
     7501 tctggaagct ggccttgacg ccggacttgt aatgccaggc aaggccgagt ttgcccacgt
     7561 tctggcggtt gacctgcgtc agcggcgaga agcgctggtt agcataatcg cggccgaaac
     7621 tgggccattc acccttggcc gcttgagcga tatggctggt cgtcgggtct ttcttgtcga
     7681 tctgtgtgca agcggagatg cccgcgagga tcaggcacaa gctggctaaa gccagaaagg
     7741 agcgagattt ggtcatggcg atatcgtgat cttggaatga acgaatttcg agtatacaga
     7801 cttgtatatg catttggcta gctgctgcag attcatcagg acgtcacaaa aattaattag
     7861 cttacgaatc attaataccg caattacttt tatactgtgg tttcatccag acaggatccc
     7921 aagccaatca tgagccttta cgccaatctc aaactcgaac accagctatg tttcgcgctc
     7981 tatgccgcga ccaatgccat cacccgtacc tatcgcggca agttaaccca ggtcggcctg
     8041 acttacccgc agtacctggt gatgatggtg ctctgggctc aggatgggat ctccgtcaag
     8101 gagatcgcgc aacgactcca gcttgactcg gcgaccatca cacccctgct caagcgcctg
     8161 gaaagtatcg gttatgtcct acgtagccgt agcagtgacg acgagcgcgt cgtgaatgtg
     8221 cggctgaccg aagacggcca cgcgatccag gagcgggtcg ccaaactgca gtccgaagtc
     8281 gaatgccaga cgcgactgga ccaggccgag ttctttgcct tgcgggcgca gttgcaccgc
     8341 ctggcggaga cactgaacca gccgccgcct gcagaagacc agcaagtcgc ctaagttttg
     8401 aatagctatt catgcataag acatcaatat ctatcaatat cttgtgtttt ttctagaact
     8461 tttgaatagg ttttgaatcg gaataaccaa tgtgagctcg ggcgtttacc caagcgccca
     8521 cccattgaga ggcatttatg aaatcgaacc agaccgccac caaagacgct ttcccgatcc
     8581 gcacggtggc cgcattgacc gggatcaacc ccatcaccct gcgcacctgg gagcgccggt
     8641 acggcttgat cgcaccgatc cgcacaccca agggacatcg cct
//
LOCUS       DATMTW010000185        15626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_587044, whole genome shotgun sequence.
ACCESSION   DATMTW010000185 DATMTW010000000
VERSION     DATMTW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 15626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15626
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587044"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /gene="ftsA"
                     /locus_tag="VN084_03480"
     CDS             complement(<1..986)
                     /gene="ftsA"
                     /locus_tag="VN084_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWT28550.1"
                     /translation="MSRVREDKNLIVGLDIGTSKIVAIVAELQPDGGLKVIGLGQHAS
                     RGLKKGVVINIDSTVNAIQRALEEAELMADCKISNVFTGIAGSHIKSLNSHGMVKIKD
                     AEVMQMDVDRVVETARAIALPSDQQILHILTQEFIIDGQEDVREPLGMSGMKLEVKVH
                     IVTGAVSAAQNIVKCIKRCGLEVTDLILQPLASSLAVLTEDEKELGVCLVDIGGGTTD
                     IAVFKQGAIRHTAVVPIAGDQITNDVAVAFRTPTQSAEEIKIKHGCALRQLADAREVV
                     EVPGVDGRDPRQLSVQTLAEVIEPRVVELYEFVLGELRRSGMEEMIASGIVIT"
     gene            complement(1015..1785)
                     /locus_tag="VN084_03485"
     CDS             complement(1015..1785)
                     /locus_tag="VN084_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HWT28551.1"
                     /translation="MWDKPILLNWIANFFYALATVLLVYGLIYVVVHLPIFPLREVRV
                     DGQLDHVTREQVKLIVDRHLRGNFFTLDLVKARDAFQKLPWARNVSVRRRWPDKLEVT
                     VEEHKELARWGNIALVNTYGELFHAASDSDLPVFYGPGDGVREVAEHYGRYSTLLGTT
                     GMKISELALTPRRAWEIKADNGMVIELGRERMEERLEKFASVYQNTLANLGVNVMYAD
                     LRYPNGFAVRRPVKTGEASGRGGPATHAAPTTNKTGAA"
     gene            complement(1775..2683)
                     /locus_tag="VN084_03490"
     CDS             complement(1775..2683)
                     /locus_tag="VN084_03490"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWT28552.1"
                     /translation="MSSGLGKVAVLMGGRSGEREVSLKSGAAVLAALQRKGVDAHAFD
                     PAERPLHDLEGFDRVFIALHGRYGEDGTIQGALELMGIPYTGSGVMASSVGMDKWRTK
                     LLWRAAGIATPDFAVVATDSDFAAIERQLGLPLFVKPANEGSSIGISKVKQAGGLKAA
                     YELAVKADPLVIAEQFVGGGEYTVGILGDHALPIIRIVPANEFYDYEAKYLRDDTQYL
                     CPCGLPAEQEARIQAEALQAFRTVGGRGWGRVDFLMDEAGKHHFLEVNTSPGMTDHSL
                     VPMGAKAAGIGFDDLVVKILEMARVG"
     gene            complement(2680..3579)
                     /gene="murB"
                     /locus_tag="VN084_03495"
     CDS             complement(2680..3579)
                     /gene="murB"
                     /locus_tag="VN084_03495"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWT28553.1"
                     /translation="MAAMQTSHGKLLRNEPLARYTSWRVGGLADRFYIPAGLEDLQAF
                     LRELPENEPVHFIGLGSNLLVRDGGVRGTVVLMHNVLNALHMEDGLVYAEAGVTCAKL
                     AKFSARQHRHGAEFMAGIPGTVGGALAMNAGCHGGETWDIVKRVATIDHRGEVRLRDQ
                     QEFVATYRHVEMAQGQPLNEWFTAAWFELPAGEASEAEQKIKNLLAKRLATQPLNMPN
                     AGSTFRNPPGDFAARLVEASGLKGHIIGGAQVSEKHANFIVNLGDATAQDIEDLITLM
                     RDTVKRQYGVDLQQEVRVIGEFA"
     gene            complement(3579..4973)
                     /gene="murC"
                     /locus_tag="VN084_03500"
     CDS             complement(3579..4973)
                     /gene="murC"
                     /locus_tag="VN084_03500"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWT28554.1"
                     /translation="MKHKVKNVHFVGIGGSGMSGIAEVLINLGFNVSGSDLADNAVTQ
                     RLAGFGARVCQGHAADHVRDADVVVISSAVKESNPEVIEARARSIPVVPRAIMLAELM
                     RFRQGIAVAGTHGKTTTTSLIASILAEAGMDPTYVIGGRLESANANARLGTGEFIVAE
                     ADESDASFLHLSPVISVVTNIDADHMDTYEHSFDKLKGAFVEFLEQLPFYGMAVVCVD
                     DPNVRAILPSISKPIMPYGFSEDARIRATNVRAEGDQMHFTVTRINGVTTSFDVVLNL
                     PGRHYVQNALAAIAVASELNVSDQAITKALKEFKGVGRRFERYGEIPAQRGGRFTLID
                     DYGHHPVEMQAVIAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVRVLSTVDEVILTE
                     VYAAGEAPIVAADGRSLAHAVGVAVRREPRFAETPAELPAAILDIARDGDVVVVMGAG
                     SIGQVAAKTREAAI"
     gene            complement(4970..6052)
                     /gene="murG"
                     /locus_tag="VN084_03505"
     CDS             complement(4970..6052)
                     /gene="murG"
                     /locus_tag="VN084_03505"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWT28555.1"
                     /translation="MSKTLLIMAAGTGGHIMPGLAVAETMRARGWQVHWLGTRHGMEN
                     KLVPARGVAMSTLDFSGLRGKGLMHTVKGLFKLVTSTLGAWKLMGDLRPSAVLGMGGY
                     VTVPGGWAAKARRLPLVLVNADAALLMSNKALLPSAKRVLFGFEGNHAVQDSHAGKIK
                     VTGNPVRREIAAIPAPAQRYADRSGPLHLLVIGGSLGAQALNRVLPEALAMIDERDRP
                     HVTHQAGEKNIAELQQAYRSAGVKADVRAFIDDMATAYAEADLLVCRAGAITVSELAV
                     AGVPSILVPLVVSTTSHQRDNAIWMAEHGGAVHLPQADMTPAGLARLLQDMTREKLLK
                     MAMHAHKLGKPDATDAIANEIEEVAL"
     gene            complement(6204..7367)
                     /gene="ftsW"
                     /locus_tag="VN084_03510"
     CDS             complement(6204..7367)
                     /gene="ftsW"
                     /locus_tag="VN084_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWT28556.1"
                     /translation="MMYMLSNRDKLISSSYDQALLWVSLILLGIGLVMVYSASIALAE
                     ADKATGHQSTYFLVRQAVYLAIGLVAAFVTFQVPTHLWQRFAPWLFIGGILLLILVLI
                     PGVGRNVNGSQRWLSLFVINLQPSEFMKLFAAIYVADYTIRKSAVMDSLTRGFLPMVA
                     VMVIVGWLLLREPDFGAFAVIAAISISILWLGGINGKIFAGLLTLLPIAIVGLIWSSP
                     YRLQRVIGFMDPWADPFGKGYQLSHALIAFGRGEWFGVGLGASVEKLLYLPEAHTDFL
                     LAVIAEELGFVGVLTVVALFAWLVLRAFRIGKEAVANERYFSALLAQGIGVWIGAQGI
                     INMGVNMGVLPTKGLTLPLLSFGGSGILANCIALAILLRIDWENRRLQKGLPA"
     gene            complement(7364..8722)
                     /gene="murD"
                     /locus_tag="VN084_03515"
     CDS             complement(7364..8722)
                     /gene="murD"
                     /locus_tag="VN084_03515"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWT28557.1"
                     /translation="MKLQLKDKRVLVLGLGDTGLSALRWLNRRGALLSVADTRDAPPG
                     LETLRAELPRVKAHLGPFQEAIFNTAELIVISPGVPMATTQVQAAIARGIPVIGDVEL
                     FAQHKPARSKVIAITGANGKSTVTTLVGEMCKAAGLSTVVAGNIGLPVLEALDAETPD
                     VYVLELSSFQLETTATLSADAAVVLNLTEDHMDRYSGMQAYAAAKARIFQGAGVQVLN
                     RGDRWSRAMAIPGRTQVTFGADTPPAPGDYGLLRRDHHTWLMQGEQALLDSAEMHVKG
                     LHNAENALAALALCRAIGLELPPLLAALRAFRGLSHRVQWVTRIHEIDFYDDSKGTNV
                     GATCAALAGMPHKVVLIAGGDGKGQDFSPLREPVAHNARAVVLIGRDAPRIAEVLQGI
                     DVPVVRLMTMEEAVTEAFRIARPNDAVLLSPACASFDMFRNYAHRADAFVSAVRKLEA
                     SA"
     gene            complement(8783..9886)
                     /gene="mraY"
                     /locus_tag="VN084_03520"
     CDS             complement(8783..9886)
                     /gene="mraY"
                     /locus_tag="VN084_03520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWT28558.1"
                     /translation="MLLELARWLAHDIRAFNVFNYITLRAVLATLTALTISFVVGPTM
                     IRKLTQYKVGQAVRDDGPQTHLIKAGTPTMGGALILVAIAIATLLWADLHNRFVWVVL
                     FTTLGFGVIGWVDDWRKVVHRNPKGLSAAAKYFWQSVIGLVVAIYLFLTTTTPAETEL
                     IVPFFKHLVIPLGAVSFIALTYLVIVGSSNAVNLTDGLDGLAIMPTVMVGSALAIFAY
                     VAGHLYFSKYLGVPYVPGAGELMVFCAAMAGAGLGFLWFNAYPAEVFMGDVVALALGA
                     ALGVVAVIVRQEIVLFIMGGVFVMETLSVAAQVSYFRYTKKKYGVGKRIFLMAPLHHH
                     YEQKGWKETQVVVRFWIITMMLVLVGLSTLKLR"
     gene            complement(9888..11303)
                     /gene="murF"
                     /locus_tag="VN084_03525"
     CDS             complement(9888..11303)
                     /gene="murF"
                     /locus_tag="VN084_03525"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWT28559.1"
                     /translation="MATQGKLIGHDVLFTSVGTDSRKLAPGQLFIALRGAHFDGHEYA
                     AKALAEGAVAVMVSASAPGVTAAVEVADTRLALGELAAHWRRKFSIPLAAITGSNGKT
                     TVKEMLAAILRAQVAQELQEQAAATLGESAHEPATVSQQAMQAVLATEGNLNNDIGLP
                     LTLLKLRAQHRYAVAEMGMNHRGEIRYLSHIGAPTVALVNNAGTAHIGELGSVEAIAQ
                     AKGEIFEGLAPDGVAVINADDVYAPLWQALAAPRKVATFGLDAPADVTAEYRLAQDHS
                     VIAMKTPIGEIAFDLPSPGLHNVRNALAAATAALAMGASLAAVAHGLAHFSGAKGRLQ
                     TKTGAHGATVIDDSYNANPASVKAAIDVLAAHAGNRLLVLGDMGELGEDAASMHAEIG
                     HYALQAGITALYALGELSRHTVSAFGQGARHYDNPQSLAAALVEVMGADTVVLVKGSR
                     FMQMERVVARITNDNTSGEAH"
     gene            complement(11324..12772)
                     /locus_tag="VN084_03530"
     CDS             complement(11324..12772)
                     /locus_tag="VN084_03530"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWT28560.1"
                     /translation="MSGPLEMLSARGVTPVGITADSRQVRPGSLFVAYPGAQRDGRDY
                     IGQAVSQGACAVLWEPEGYHWDERLQVPNLAVSGLRAAAGDIADRFYGEPSRALWMIG
                     VTGTNGKTSCSQWLAQALNILGRKTAVVGTLGNGFPGALSAAINTTPDPILLQGMLAD
                     YLEQGASGVAMEVSSHGLDQGRVNGVHFDVAVFTNLSRDHLDYHGDIASYAAAKRKLF
                     VWPGLAHAVVNSDDAFGRELADQLAFAGKPVLTYGLDSGDVRASALVFNDRGLSMRID
                     SPFGAAKLEAGVAGRFNAYNLLAVLSTLLVSGVPLAQVVAAMAELRAVPGRMQQAGGG
                     ALPLVVVDYAHTPDALEKVLQALRTQTRGQLICVFGCGGDRDAGKRPLMGQIVGQLAD
                     RALVTSDNPRSEDPQAIIKAIVADMTGDYHIEPDRAVAITEAILNAGPGDVVLVAGKG
                     HEAYQEVAGVRYPFSDLDVAERALQQHKRRPA"
     gene            complement(12769..14499)
                     /locus_tag="VN084_03535"
     CDS             complement(12769..14499)
                     /locus_tag="VN084_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWT28561.1"
                     /translation="MNHAATQQLMIKLPAWRRRVLLIAVLAGFAVLLGRGVYLQGMHK
                     SFLQKEGDARYSRVITLPAHRGMITDRYGEPLAISTPVESIWANPSDVKITAEQSARL
                     AKLLDLKPADLSKKLTNSEREFVYIKRRIPPELAHQVMRLGISGVFMQREYRRYYPAG
                     EVTAHLVGFTGIDDNGQEGFELKYQNWLSGKPGSRRVIKDRQGHIVEDLEAVKVPQDG
                     KDLVLSIDRKIQYLAYRELASAVELHKAKAGAAIVLDAKTGEVLAMVNLPTYNPNNPV
                     RIAGKSRNRAIVDMFEPGSTLKPVTAAAALETGTFTPETTIDTAPGTLRIGPATIHDA
                     HREGVLTVAQVIQKSSNVGSSKIALTLQPQYLWSVFNRIGLGAPTHIGFPGEAAGIVR
                     NYKTWRPIEQATMSFGHGISLTLLQLARAYTVFANEGELRPVSLLKLSESPVGQQVFS
                     AKTANSVKDMLELAVLPGGTAPRAQVLGYRVAGKTGTAHKLGPGGYLPNSYVSSFVGM
                     APASNPRLIIAVMIDEPTTGSYYGGLVAAPVFSNIMSGTLRMLAIPQDAPNNNIVIPP
                     EDAPIIKEDV"
     gene            complement(14496..14792)
                     /gene="ftsL"
                     /locus_tag="VN084_03540"
     CDS             complement(14496..14792)
                     /gene="ftsL"
                     /locus_tag="VN084_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWT28562.1"
                     /translation="MTRLNLILFFVLIATAIGSITAQHKSRKLYFELQQQQDAAKQYD
                     VEWGQLQLEQSTWAMHARIEQIASRHLQMQVPDAKRIQVVVPPVAVHEDVKAAP"
     gene            complement(14797..>15626)
                     /gene="rsmH"
                     /locus_tag="VN084_03545"
     CDS             complement(14797..>15626)
                     /gene="rsmH"
                     /locus_tag="VN084_03545"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWT28563.1"
                     /translation="SRLILQKLGGRGRLIALDRDLAAIRAAAAIADPRFMIVHRHFSE
                     MSEALQALGVSKVQGVLLDLGISSPQIDTGDRGFSFRFDGPLDMRMDQSRGQTAAAFI
                     AEATEQQLTGVIRDYGEERFAKQIARAVITARTGGQPVTTTGQLAKIVAGAVPKLEPG
                     QDPATRTFQALRIFLNQELEELALVLPQCMQLLSPGGRLAVISFHSLEDRIVKRFIRG
                     EQDRDDLPANFPVRAKDLPQPRLMAVGRAIKPSAAEVKRNPRSRSAVLRVAERTAEA"
BASE COUNT         2887 a         5026 c         4793 g         2920 t
ORIGIN      
        1 gtgatgacga tgccggaagc gatcatctct tccatgccgc tacggcgcag ctcgcccaat
       61 acgaactcat acagctcgac cacacgtggt tcgatcacct cggccagcgt ctgtaccgag
      121 agctgacgcg ggtcgcgacc gtcgacaccc ggcacctcga ccacttcacg tgcatcggcc
      181 agttggcgca gcgcgcagcc gtgcttgatc ttgatctctt cagccgattg cgtcggtgtg
      241 cggaacgcca cggcgacgtc gttggtgatc tggtcgccgg cgatggggac cacagccgta
      301 tggcgaatgg cgccctgctt gaacaccgcg atatccgtcg tgccgccgcc gatgtcgacg
      361 aggcacacac cgagttcctt ctcgtcctcg gtcagcacgg cgaggctcga ggccagcggc
      421 tgcagaatga ggtcggtgac ctcgagaccg cagcgcttga tgcacttgac gatgttctgc
      481 gcggcggaga ctgccccggt gacgatatgc accttcactt ccagtttcat gccgctcatg
      541 cccagcggct cccgcacgtc ttcctggccg tcgatgatga attcctgcgt caggatgtgc
      601 aggatctgct gatcgctcgg caacgcgatg gcgcgcgccg tctcgaccac gcggtcgaca
      661 tccatctgca tcacttcggc atccttgatc ttgaccatgc cgtgagaatt caagctcttg
      721 atgtggctgc cggcgatgcc ggtgaagaca ttgctgatct tgcagtccgc catcagctcg
      781 gcttcttcca gcgcacgctg gatggcgttg accgtggaat cgatattgat cacgacgcct
      841 ttcttcaaac cgcgcgacgc gtgctgaccg aggccgatga ccttgagccc gccgtccggc
      901 tgtaactcgg cgacgatggc gacgatcttg gatgtcccga tatccaggcc gacgatcagg
      961 ttcttgtctt ctctcactct gctcataagg tgctccatgt ttggccgcgc atcatcacgc
     1021 cgcccctgtt ttattcgttg tcggcgccgc atgcgtcgct ggcccgccac ggccggacgc
     1081 ttcgcccgtc ttgaccggcc gccgcacggc gaagccgttg gggtagcgca ggtcggcata
     1141 catcacgttc acgccgaggt tggccagcgt gttctgatag acgctggcga acttctccag
     1201 ccgctcttcc atgcgctccc gccccagctc gatgaccatg ccgttgtcgg ctttgatctc
     1261 ccatgcccgc cgtggcgtca gtgccagttc gctgatcttc atccccgttg tgccaagcag
     1321 cgtgctgtat ctgccgtagt gttcggccac ctcgcgcacg ccgtcgcctg gtccgtagaa
     1381 caccggcaag tcactgtcag aagcggcatg gaacagctcg ccatacgtgt tcaccagcgc
     1441 gatgttgccc cagcgcgcca gctccttgtg ctcttccacg gtcacttcca acttgtccgg
     1501 ccagcgccgg cgcacactga cattccttgc ccacggcagc ttctggaagg cgtcgcgcgc
     1561 ctttaccaaa tccaatgtga agaagttgcc gcgtaaatga cggtccacga tcagcttcac
     1621 ctgctcacgc gtcacatggt cgagctgtcc atccacccgc acctcgcgca aggggaagat
     1681 cggcaggtgc acgaccacat agatcaggcc gtagaccagc agcacggttg ccaacgcgta
     1741 aaaaaagttg gcgatccagt tcaacaaaat gggcttatcc cacacgcgcc atctccagta
     1801 tcttcactac cagatcatca aacccgatgc ccgctgcctt ggcgcccatg ggcaccaagc
     1861 tgtggtcagt catgccgggc gaagtgttca cttccaggaa gtgatgcttg ccggcctcgt
     1921 ccatcaggaa gtcgacccgc ccccagccac ggccacccac ggtacggaac gcctgtagcg
     1981 cctcggcctg gatgcgcgcc tcctgctccg caggcaagcc acaagggcag agatactgtg
     2041 tgtcgtcgcg caggtacttg gcttcatagt cgtagaactc gttggctggc acgatgcgga
     2101 tgatcggcaa cgcgtgatcg ccaaggatgc cgaccgtgta ttcgccgcct ccgacgaact
     2161 gttccgcgat cactaacggg tctgccttga cggcgagttc ataggccgcc ttgaggccgc
     2221 cggcctgctt caccttgctg atgccgatgc tagaaccctc gttggctggc ttgacgaaca
     2281 gcggcaagcc gagctgccgc tcgatcgccg cgaaatcgct gtcagtcgcg accacggcaa
     2341 aatccggggt ggcgatgccg gccgcacgcc aaagcagctt ggtgcgccac ttgtccatgc
     2401 ccacgctgga agccatgaca ccgctgccgg tataggggat gcccatgagc tcgagcgcgc
     2461 cctggatggt gccgtcttca ccgtagcggc catgcagggc gatgaatacg cgatcaaagc
     2521 cttcaaggtc atgcaatggc cgctctgccg ggtcaaacgc gtgtgcatcc acgcccttac
     2581 gctgcaaggc ggccagcacg gcggcaccgc tcttcagcga gacttcacgc tcgcctgaac
     2641 gtccacccat caacaccgcc acttttccca acccgctact catgcaaact ctccaatgac
     2701 acgtacttcc tgctgcaaat ccacgccgta ttgccgcttc accgtatcgc gcatcagcgt
     2761 gatcagatcc tcgatgtcct gtgccgtcgc atcgccgagg ttgacgatga agttggcatg
     2821 tttctccgag acctgtgcac cgccgatgat gtggcccttg agcccgctgg cttcgaccag
     2881 tcgtgccgcg aagtcgcccg gcggattgcg aaaggtcgaa cccgcgttcg gcatgttcag
     2941 gggctgtgtt gccaaccttt tcgccagcag gttcttgatc ttctgctcgg cctcgctggc
     3001 ttcaccggca ggtaattcga accaggcggc ggtgaaccat tcgttgagcg gctgtccctg
     3061 cgccatctcg acatgccgat aggtggccac gaattcctgc tggtcgcgca accgcacttc
     3121 accccgatgg tcgatggtcg ccacgcgctt gacgatgtcc caggtctcgc cgccatggca
     3181 gccggcgttc atcgccagcg caccgccgac cgtacccggg atgccggcca tgaactcagc
     3241 gccatgccga tgctggcggg cactgaattt ggctagcttg gcgcaggtga cgccggcttc
     3301 ggcatagacc aggccgtcct ccatatgcag tgcattcagc acgttatgca tgagcaccac
     3361 ggtgccgcgc acgccgccat cacgcaccag caggttggaa ccgaggccga tgaaatgcac
     3421 cggctcgttt tccggtagtt cgcgcaggaa ggcttgcaga tcttccagtc ccgccgggat
     3481 gtagaaacgg tcagccaagc cacccacgcg ccagctggtg tagcgagcca gcggctcatt
     3541 gcgcagcagc ttgccgtgcg atgtctgcat ggcggccatc aaatcgcagc ctccctggtt
     3601 ttggcggcga cctggccgat agagcctgcg cccatgacca cgaccacatc accgtcccgt
     3661 gcgatatcga ggatagcggc aggcaattcg gccggcgtct cggcgaagcg tggttcacga
     3721 cgcacggcca caccgaccgc atgcgccagc gaacggccgt cggcggcgac gatgggcgct
     3781 tcgccagcgg catagacttc ggtcaggatc acttcgtcca ccgtcgacag aacgcgtacg
     3841 aaatcctcga aacaatcgcg ggtacgcgta tagcgatgcg gctggaaagc cagcaccagc
     3901 cggcgacccg ggaaggcccc acgcgccgca gcgatcacgg cctgcatctc gaccgggtga
     3961 tggccataat cgtcgatcag cgtgaagcga ccgccacgtt gtgcagggat ctcgccatag
     4021 cgctcgaagc ggcggccgac gcccttgaat tccttcagcg ccttggtgat ggcctggtcg
     4081 gagacgttca attcgctggc gacggcgatg gcagccagcg cgttctgcac gtaatgccgg
     4141 cccggcaggt tgagtacgac atcaaaactg gtggtgacac cgttgatgcg cgtcaccgtg
     4201 aaatgcatct ggtcgccctc ggcgcgcacg ttggtagcgc ggatgcgggc atcctcactg
     4261 aagccgtaag gcatgatggg tttggagatg ctgggcagga tggcgcgcac gttgggatca
     4321 tcgacgcaga ccaccgccat gccgtagaac ggcagttgtt cgaggaactc gacgaaagcg
     4381 cccttgagtt tgtcgaagct gtgctcgtag gtgtccatgt ggtcggcatc gatattggtc
     4441 acgaccgaga tcaccggcga caggtgcagg aacgaggcgt cggattcgtc ggcctcggcg
     4501 acgatgaact cgccagtgcc cagccgcgcg ttggcattgg cggattccag acggccgccg
     4561 atgacatagg tcggatccat accggcttcg gccaggatgc tggcgataag gctggtggtc
     4621 gtggtcttgc cgtgcgttcc ggcgacggcg atgccctgcc ggaagcgcat gagttcagcc
     4681 agcatgatgg cgcgcggcac cacggggatg ctgcgtgcgc gggcttcgat cacctccggg
     4741 ttgctctcct tgaccgcaga ggagatgacg accacgtcgg catccctgac gtggtctgcg
     4801 gcatgcccct ggcaaacgcg tgcgccgaaa ccggcaagcc gctgggtcac agcgttgtcg
     4861 gcgaggtcag aaccgctgac gttgaagccg aggttgatca gcacctcggc gatgccgctc
     4921 atgcccgagc cgccaatacc gacgaaatgc acgttcttga ccttatgttt cacaaggcca
     4981 cctcctcgat ctcgttcgcg atcgcgtccg tggcatccgg cttgcccagc ttgtgggcat
     5041 gcatcgccat cttcagcagc ttctcacgcg tcatgtcctg taaaagccgg gccagcccgg
     5101 cgggcgtcat atccgcttgc ggcagatgca ccgcgccgcc atgttctgcc atccagatgg
     5161 cgttgtcgcg ttgatgcgag gtggtcgaga ccaccagcgg cacaaggatg ctgggcacac
     5221 cagccactgc cagctcgctc accgtgatcg cgcctgcacg acataccagt agatcggcct
     5281 cggcataagc cgtagccata tcgtcgatga aggctcgcac atcggccttg acccctgcgc
     5341 tccggtaagc ctgctgtaat tcagcgatgt tcttttcgcc tgcctgatgc gtgacatgcg
     5401 gcctatcacg ctcgtcgatc atggccaacg cttcaggtag cacccggttc agcgcctgcg
     5461 cgcccaggct accgccgatg accagcaggt gcagtggccc cgaccgatcg gcgtagcgct
     5521 gtgcaggcgc agggatggcc gcgatctcac gacgtactgg gttacctgtg accttgatct
     5581 tgccggcatg gctgtcttgg actgcatggt tgccttcaaa gccgaacagc acgcgctttg
     5641 ccgaaggcaa cagcgccttg ttcgacatca gcaaggctgc atcggcgttg accagcacca
     5701 agggcagcct gcgtgccttg gccgcccaac cgccgggaac cgtcacataa ccgcccatgc
     5761 ccagcacggc gctcggacgc agatcaccca tgagcttcca ggcgcccagc gtggaagtca
     5821 caagcttgaa gaggcctttg acggtatgca tcagtccctt gccgcgcagg ccggagaaat
     5881 ccagcgtgct catggcgacg ccgcgcgctg gcaccagctt gttctccatg ccgtgtcgcg
     5941 tgcccagcca atgcacctgc cagccacgcg cgcgcattgt ttcggcgacc gcgaggccgg
     6001 gcatgatgtg cccgccagta cccgccgcca tgatcaggag ggtcttgctc atgccgcgcc
     6061 ctcctccgtt tgacggatgt atagactccg ttcgagttgg ggatttttgg ctacggccgc
     6121 tgcgcttaac ctgcagacgc aggttagcct acgccggaat aggcgctcta tgatgctagc
     6181 gtgcagatat gagaatagcg ccatcatgcg ggcagcccct tttgtaagcg cctattctcc
     6241 caatcgatcc gaagcaggat cgccaacgcg atgcagttgg cgaggatgcc gctgccgccg
     6301 aacgaaagca gcggcagggt cagccccttg gtcggtagca cccccatgtt gacccccatg
     6361 ttgatgatgc cctgtgcacc gatccagacg ccgatcccct gtgccagcaa agccgagaag
     6421 taacgctcat tggcgacggc ctccttgccg atgcggaagg cccgcagcac gagccaggca
     6481 aagagggcga cgacggtgag cacgccgacg aatccgagtt cttcggcaat cacggcgagc
     6541 aggaaatcgg tatgtgcttc cggtaggtat aagagcttct cgacgctggc gccgaggccg
     6601 acgccgaacc attcgccgcg accaaaggcg atcagcgcat gtgacagctg gtagcccttg
     6661 ccgaacgggt cggcccatgg gtccatgaaa ccgatcacgc gctgcaggcg gtaaggcgaa
     6721 ctccagatga ggccgacgat cgcgatcggc agcaaggtca gcaaaccggc gaagatcttg
     6781 ccgttgatgc cgccaagcca aagaatggag atggagatgg cggcgatcac ggcaaaggcg
     6841 ccaaagtcag gttcgcgcag cagcagccag ccgacgatca ccatgaccgc caccatgggc
     6901 agaaagccgc gcgtcaggct atccatcacg gccgatttgc ggatggtgta atccgccacg
     6961 tagatggcgg cgaacagttt catgaattcc gacggctgca ggttgatgac gaacagcgat
     7021 agccagcgct ggctgccgtt gacgttgcgg ccgaccccgg ggatcagcac caggatcagc
     7081 aacaggatgc cgccgatgaa cagccatggc gcgaagcgct gccagaggtg ggtcggcacc
     7141 tggaaggtga cgaaggcggc gaccagaccg atggccagat acaccgcctg ccgcaccagg
     7201 aaataggtgc tttggtgccc ggtcgccttg tcggcctcgg ccagtgcgat cgatgccgaa
     7261 tacaccatga ccagcccgat gccgagcaag atcaggctga cccagagtaa ggcctggtca
     7321 taactcgagg agatcagctt gtcacgattg gagagcatgt acatcacgcg ctggcctcca
     7381 gttttcgcac tgcgctgacg aatgcatcgg cacgatgcgc ataattgcgg aacatgtcga
     7441 agctggcgca ggctggcgac aacaggaccg cgtcattcgg cctggcgatg cggaaagctt
     7501 cggtgacggc ctcttccatg gtcatgagcc gcaccacagg cacatcgata ccctgcagga
     7561 cctcggcgat gcgaggtgcg tcacgcccga tcagcaccac ggcacgggcg ttatgcgcca
     7621 ccggctcgcg tagcggcgag aaatcctgac ccttgccatc gccgccggcg atcagcacga
     7681 ccttgtgcgg catgccagcc agcgcagcgc aagtggcgcc gacgttggtg ccctttgaat
     7741 cgtcatagaa atcgatctcg tggatacgcg tgacccactg gacccggtgc gacaaaccgc
     7801 ggaacgcgcg cagcgctgcc agcaggggcg gcaattccag gccgatggcc cggcacagcg
     7861 ccagggccgc gagcgcgttc tctgcattgt gcaggccctt gacgtgcatc tcggcgctat
     7921 cgagcaaggc ttgctcgccc tgcatcagcc aggtgtgatg gtcacgtcgc agcaggccat
     7981 aatcgccggg cgcgggcggt gtatcggcac cgaaggtcac ctgggtgcgg cctgggatcg
     8041 ccattgcacg gctccagcgg tcaccccggt tgagcacctg cacgccagcg ccctggaaga
     8101 tacgggcctt ggctgcggcg taagcctgca tgccgctgta tcggtccatg tgatcctcgg
     8161 tcaggttgag gaccaccgcc gcatcggcag agagcgtggc tgtcgtctcc agttggaaac
     8221 tggacagctc gaggacatag acatcgggtg tttcggcgtc gagcgcctcc agtaccggca
     8281 ggccgatatt gccggcgacc actgtgctca agccggccgc cttgcacatc tcgccgacca
     8341 gggtggtgac cgtgctcttg ccgttggcgc cagtgatggc gatcactttc gaccgcgccg
     8401 gcttgtgttg tgcgaatagc tcgacatcgc cgatcacggg gatcccgcgc gcgatcgcgg
     8461 cttgcacctg cgttgtcgcc atgggtaccc cgggactgat gacgatgagt tcggccgtgt
     8521 tgaagatcgc ctcctggaat ggaccgagat gggccttgac gcgcggcaac tccgcccgta
     8581 gggtctccag ccccgggggg gcatcgcgcg tatcggcgac ggacagcagg gcaccgcggc
     8641 gattgagcca gcgcagcgcc gaaagaccgg tgtcacccag ccccagcacc aatacccgtt
     8701 tgtcctttaa ttgcaacttc atccctgtct cgatcgctcg ttcatccgtg gcttgttatc
     8761 tatggctttt cgcgtcagcg gcttatctca gcttcaacgt cgaaagcccg accagcacca
     8821 gcatcatcgt gatgatccag aacctcacca cgacttgcgt ctctttccag cccttctgtt
     8881 cgtaatggtg gtgcagcggc gccatgagga agatgcgctt gcccaccccg tacttcttct
     8941 tggtgtagcg gaagtaactg acctgtgccg ccaccgacag cgtctccatg acgaacacgc
     9001 cgcccatgat gaagagcacg atctcctgtc gcacgatgac ggcgaccaca ccgagcgcgg
     9061 cccccagcgc cagcgccacg acatcaccca tgaagacctc ggccggatag gcgttgaacc
     9121 agaggaagcc caggccggca cccgccatcg ccgcgcagaa caccatgagt tcaccggcgc
     9181 ctggcacgta gggcacgcca agatatttgg agaaatagag gtgaccagcg acataagcga
     9241 agattgccag tgcgctgccg accatcacgg tcggcatgat ggcaaggccg tctagaccgt
     9301 cggtgaggtt gacggcatta ctgctgccga cgatgaccag ataggtcaac gcaatgaaac
     9361 tgacagcccc gagtggaatg acgagatgct tgaagaacgg cacgatcagc tccgtctcgg
     9421 ccggtgtcgt cgtcgtcagg aacaggtaga tcgcgaccac aaggccgatc accgactgcc
     9481 agaaatactt ggcggccgct gacagcccct tgggattccg atgcacgacc ttgcgccagt
     9541 catcgaccca accaatgacg ccaaaaccca gcgtggtgaa caacaccacc cagacgaagc
     9601 gattgtgcag atcggcccag agcaaggtgg cgatggcgat ggcgaccagg atcagggccc
     9661 cgcccatggt cggcgtgcca gccttgatca gatgcgtctg tgggccgtcg tcacgcacgg
     9721 cctggcccac cttatactgc gttagcttgc ggatcatggt cggaccgacg acgaaagaga
     9781 tggtgagcgc tgtcagcgtc gccagcacgg cgcgcagcgt gatgtaattg aagacattga
     9841 atgcgcgtat gtcgtgcgcc agccagcgtg cgagttcgag tagcatccta gtgggcctcc
     9901 ccagaggtgt tgtcgttggt gatgcgcgcg accacgcgct ccatctgcat gaaccgtgag
     9961 ccctttacca gcaccaccgt atccgcgccc atgacctcga ccaaggcagc agcaaggctt
    10021 tgcgggttgt cgtaatgacg ggcaccctgt ccgaacgcgg agaccgtatg ccgactcaat
    10081 tcgcctagcg catagagcgc ggtgatgccg gcctgcaggg catagtggcc gatctcagcg
    10141 tgcatgctgg cggcgtcttc gcccagctcg cccatgtctc ccaggaccag cagcctgttg
    10201 cccgcgtggg ctgccaacac gtcgatcgcc gccttgaccg aggcaggatt ggcgttgtag
    10261 ctatcgtcga tgacggtggc gccgtgagcg cccgtcttgg tctgcaagcg ccccttggcg
    10321 ccactgaaat gcgccagtcc atgcgccacc gcggccaggg aagcacccat ggccagtgcg
    10381 gctgtcgcgg cggcgagtgc gtttcgaacg ttgtgcaacc ctgggctcgg cagatcgaat
    10441 gcgatctccc cgatcggcgt cttcatggcg atgacgctat ggtcctgcgc cagccgatac
    10501 tccgcggtga cgtctgccgg cgcgtcgaga ccgaaggtcg ccaccttgcg cggcgcagcc
    10561 agcgcctgcc acaagggggc gtagacgtcg tcggcattga tgacggcgac cccgtccggc
    10621 gccagcccct caaagatctc gcctttggct tgcgcgatgg cttccaccga acccagctcg
    10681 ccgatatgag ccgtgcccgc gttattgacc agcgccacgg taggcgcccc gatatggctc
    10741 agatagcgaa tctcgccgcg atggttcatg cccatctcag ccacggcata gcggtgctgg
    10801 gcgcgcagct tgagcaaggt cagcggcagg ccgatatcgt tgttgaggtt accttctgtc
    10861 gccaggacgg cttgcatcgc ttgctgcgag accgtggcgg gctcatgcgc gctttcgccc
    10921 agcgttgcgg cagcttgctc ttgcaactcc tgggcgacct gcgcccgcag aatggcggcc
    10981 agcatctctt tcaccgtggt cttgccattg ctgccggtga tcgccgccag cggaatgctg
    11041 aacttgcggc gccaatgggc ggccagctcg ccaagcgcca atcgggtatc ggcaacctcg
    11101 accgcggcgg ttacgcccgg tgccgaggca gagaccatga cggcgacggc gccttccgcc
    11161 agtgccttgg cggcatactc atgaccgtcg aaatgggcgc cgcgcagggc gatgaagagc
    11221 tggcccgggg ccagcttgcg gctatcggta ccgacactgg tgaacaagac atcgtggccg
    11281 atgagcttgc cttgcgtcgc cattgcggct tccgagagat gcatcatgcc ggcctccgct
    11341 tgtgttgctg cagcgcgcgc tcggcgacgt cgagatcgct gaacgggtaa cgcaccccgg
    11401 cgacctcctg gtaggcctcg tgccctttgc ccgccactag tacgacatcg ccggggcctg
    11461 cgttgaggat ggcttcggtg atggcgacgg cgcgatccgg ctcgatgtgg taatcgccgg
    11521 tcatgtcggc gacgatggcc ttgatgatgg cctgcggatc ctcactgcgc gggttgtccg
    11581 aggtcaccag cgcgcggtcc gccagctgac cgacgatctg ccccatgagc gggcgcttgc
    11641 ccgcgtcgcg gtcgccgccg caaccgaata cgcagatcag ctgacctcgc gtttgtgtac
    11701 gcagggcttg caggactttt tccagcgcgt ccggcgtatg cgcatagtcg accaccacca
    11761 gcggcaatgc gccgccaccg gcctgctgca tgcgcccggg cacggcgcga agctcggcca
    11821 tggcggcgac cacctgcgcc agcggcacgc cggagacgag caaggtggac agcaccgcca
    11881 acaggttgta cgcgttgaaa cgaccagcca ccccggcttc gagcttagcg gctccgaagg
    11941 gactatcgat gcgcatcgaa agtccacgat cgttgaagac aagcgcgctc gcgcgcacgt
    12001 cgccgctgtc cagcccgtag gtcaggaccg gcttgccggc gaatgccagc tggtccgcca
    12061 gttcgcgacc gaaagcatcg tcggagttga ccaccgcatg cgccaggcca ggccagacga
    12121 acagcttgcg cttggcggcg gcgtagctcg cgatgtcgcc gtggtaatca agatggtcgc
    12181 gtgacaggtt ggtgaatacg gcgacatcga aatgcacgcc gttcacacgc ccctgatcga
    12241 gaccgtgcga agagacctcc atggcgacgc cagatgcgcc ctgttcgaga taatccgcca
    12301 gcatgccctg cagcaggata gggtcgggcg tggtgttgat ggctgccgag agcgcgccgg
    12361 ggaagccgtt accgagcgtg ccgacgactg ccgtcttgcg gccgaggata ttcagcgcct
    12421 gcgccagcca ctggctgcac gaggttttgc cattggtgcc ggtgacaccg atcatccaca
    12481 gcgcgcgcga aggctcgcca tagaaacggt cggcgatgtc gccggccgcg gcacgcagtc
    12541 cggacacagc aaggttgggc acttgcaagc gctcgtccca gtggtatccc tctggctccc
    12601 ataacacggc gcatgcgcct tgcgacaccg cctggccgat atagtcacgc ccatcacgct
    12661 gtgcgcccgg gtatgccacg aacaggctac ctggcctcac ctgtcggcta tccgcggtga
    12721 taccgacagg cgtgacgccg cgtgcgctta acatctccag aggaccgctc atacgtcctc
    12781 cttgatgatc ggcgcgtctt ccggcgggat gacgatattg ttgttaggcg catcctgcgg
    12841 gatggccaac atacgcaatg tccccgacat gatgttgctg aacaccggcg cagccaccag
    12901 gccgccatag taggaacccg tcgtcggctc gtcgatcatg acagcgatga tcagccgtgg
    12961 attggaagcc ggcgccatgc cgacgaagga cgatacgtaa ctgttaggca agtagccgcc
    13021 aggaccgagt ttatgggccg taccggtctt gcctgcaacg cggtagccaa gcacctgcgc
    13081 ccttggggca gtgccgccgg gcaacaccgc aagctccagc atatccttga cgctattggc
    13141 ggtcttggcc gagaacacct gctgccccac tggtgattcg gagagtttga gcaggctcac
    13201 cggccgcagc tcgccctcgt tggcgaacac cgtgtaggcc ctggccagtt gcagcagtgt
    13261 caggctgatg ccatggccga acgacatggt ggcctgttcg atgggacgcc aggtcttgta
    13321 attgcggacg atgcctgcag cctcgcccgg gaagccgata tgcgtcggcg cgccaaggcc
    13381 gatgcggttg aacacgctcc atagatattg cggctgcaac gtcagcgcga tcttcgacga
    13441 gccgacgttc gatgatttct ggatgacctg cgccaccgtc agcacgccct ccctgtgggc
    13501 gtcatgtatg gtcgcgggcc cgatacgcaa ggtgcccggc gcggtatcga tcgtggtctc
    13561 gggggtgaac gtcccggttt ccagcgctgc cgcggcagtc accggcttta gggtcgagcc
    13621 tggctcgaac atgtcgacga tcgcgcgatt acgggatttg ccggcgatcc gcaccgggtt
    13681 gttggggtta taggtgggca gattgaccat cgccagcact tcgccagtct tggcgtcgag
    13741 cacgatggcg gcacccgcct tggccttgtg caattcgacg gcgctggcca gctcacgata
    13801 agcgaggtac tggatcttgc ggtcgatgga gagcacgaga tccttgccgt cctgcggcac
    13861 cttgaccgcc tccagatcct cgacgatatg gccttgacgg tccttgatca cgcggcggct
    13921 acccggtttg ccggacaacc agttctgata cttgagctcg aagccctcct ggccgttgtc
    13981 gtcgatgccg gtgaagccga ccaggtgcgc ggtgacttcg ccagccgggt agtagcggcg
    14041 gtattcacgc tgcatgaaga cgccagagat gccgagacgc atgacctgat gcgctagttc
    14101 gggcgggatg cgccgcttga tatagacgaa ttcgcgttcg ctattggtga gtttcttgct
    14161 gagatcggcc ggcttgaggt ccagcagttt agccagcctg gcactctgtt cagccgtgat
    14221 cttgacatcg gacgggttgg cccagatcga ttccaccggc gtgctgatgg ccagcggctc
    14281 gccataccgg tcagtgatca tgccccgatg tgcgggcaag gtgatgacgc ggctatagcg
    14341 tgcatcgccc tctttctgca ggaagctctt gtgcatgccc tgcaggtaga caccccgccc
    14401 cagcaagacc gcgaagccag ccagcaccgc gatcagcagg acgcgacgac gccatgcagg
    14461 cagcttgatc atgagttgct gggtcgcggc gtggttcatg gcgcagcctt cacgtcttca
    14521 tggaccgcca cagggggaac gacgacctgg atacgcttgg catccggcac ctgcatctgc
    14581 agatgccggc tggcgatctg ttcgatacgc gcgtgcatcg cccatgtgct ctgctccagc
    14641 tgcagctgtc cccattcgac gtcgtattgc ttggccgcat cctgttgttg ctgcagctcg
    14701 aaatagagct tgcgcgactt gtgctgggcc gtgatcgagc cgatggcagt cgcgatgagc
    14761 acaaagaaca ggatgaggtt gagtcgggtc atgcgcttac gcctccgccg ttctctcggc
    14821 gacacgcagc actgcgctgc gtgagcgggg gttgcgcttc acttcggccg ccgagggctt
    14881 gatggcacga ccgaccgcca tcaaccgcgg ctgcggcaga tccttggcac gcaccgggaa
    14941 attggcaggc aggtcgtcgc gatcctgctc gccccggata aagcgcttga cgatacgatc
    15001 ctccagcgaa tggaagctga tgaccgccag gcgaccgccc gggctcagca actgcatgca
    15061 ttggggcagg actagcgcca attcctcaag ctcctgattg aggaaaatcc gtaaagcttg
    15121 aaaggtgcgc gtcgccggat cttggccagg ctcgagtttg ggcaccgcgc ctgccacgat
    15181 cttggcaagt tgtccggtag tggtgacggg ctgcccccct gtgcgtgccg taataaccgc
    15241 ccttgcaatc tgtttagcaa accgttcttc accataatct ctgatcaccc ctgtcaattg
    15301 ttgctctgtt gcctcagcga tgaacgccgc cgctgtttgt ccgcggcttt gatccatgcg
    15361 catgtcgagc gggccgtcga acctgaaact gaacccacga tcccctgtat cgatctgcgg
    15421 tgaggagatc cccagatcca gtaagacgcc ttgcaccttg ctcacaccca gcgcctgcaa
    15481 ggcttcgctc atctcggaaa aatgccgatg cacgatcatg aaacgcgggt cagcgatcgc
    15541 tgccgccgca cgtatggccg caaggtctcg gtcaagcgcg atcagccggc cccggccacc
    15601 cagcttctgc aggatgagac ggctat
//
LOCUS       DATMTW010000186         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_84031, whole genome shotgun sequence.
ACCESSION   DATMTW010000186 DATMTW010000000
VERSION     DATMTW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84031"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VN084_03550"
     CDS             complement(<1..1310)
                     /locus_tag="VN084_03550"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWT28564.1"
                     /translation="MSLDSTSNSSLHHVVIVGGGAGGLELATRLGDSLERKGKAHITL
                     IDRNRTHVWKPLLHEIAAGSMNPDKHELEYLAQAHWHHFYFRLGNMDGIDRSKKEVYV
                     APYHDEDGVEVIPRRTFRYDTLIVAVGSTTNDFGIAGASEYSIALDTQGQAERFHRRL
                     HNALVRAQTQVEPVQAGQLEVVIVGAGATGVELAAELHNTTRELTAYGLDKIDPDRDV
                     RISLIEASDRVLPALPPRLSKKVDNELRKLKVHLYNGERVTEVTEKGVYTHSGRFIPS
                     ELVVWAAGIKAPDFLAQLDGLETNRLNQLVVTSTLQTTIDENIFAFGDCAACPWPGHV
                     ENVPPRAQAAHQQASMLVKTVKRRIANKPLPEYVYRDYGSLVNLGRYTTVGNLMGAVT
                     GGSMFIEGLIASFMYQSLYKMHLMTLHGFFTVMLQTLARIITRRT"
     gene            complement(1340..>1511)
                     /locus_tag="VN084_03555"
     CDS             complement(1340..>1511)
                     /locus_tag="VN084_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HWT28565.1"
                     /translation="TNKGMFETIWHGTNGGMGAKGKGLMDPTDPANGLSPDEVLKIIA
                     WIRSHGNATGNE"
BASE COUNT          315 a          435 c          420 g          341 t
ORIGIN      
        1 gtccgtcgcg tgatgatccg cgccaaggtc tgtaacatca ccgtaaagaa cccatgtaat
       61 gtcatcagat gcatcttgta caaggactga tacatgaagc ttgcgatcag gccttcaatg
      121 aacatactac cgccggtgac cgcacccatc aggttgccga ccgtggtata gcgaccaaga
      181 ttgaccaatg agccgtagtc acggtagaca tattcaggca gaggcttgtt cgcgatccga
      241 cgtttcactg tcttgaccag catcgaggct tgctggtggg cagcctgcgc gcgcggcgga
      301 acattttcga catggccggg ccacggacag gccgcgcaat cgccgaacgc gaagatgttc
      361 tcgtcaatcg tggtttgcag cgtgctggtc accaccagct gattcaagcg gttggtttca
      421 aggccatcca gctgagccag gaaatccggg gccttgatgc ccgcggccca gaccaccagt
      481 tcggagggga tgaagcgtcc actatgggta tagacgccct tttccgtcac ctcagtcacg
      541 cgctcaccgt tatatagatg taccttgagc ttgcgcagct cgttatcgac cttcttcgac
      601 aggcgtggtg gcagcgccgg caatacacgg tcactggcct cgataagcga gatacgcaca
      661 tcacgatccg gatcgatctt gtcgaggccg taagcggtga gctcacgtgt cgtgttatgc
      721 aattcggcag cgagttcgac acctgttgca ccggcgccga cgatcacgac ttccagctgg
      781 ccagcctgta ccggctccac ctgcgtctgt gcacgcacca aggcgttatg caaacgacga
      841 tggaagcgtt cggcctggcc ttgcgtatca agggcgatcg aatactcact ggcgccggcg
      901 ataccgaaat cattggttgt gctgccgacc gcaacgatca acgtgtcata acggaaggtg
      961 cggcgtggga tcacttccac gccgtcctcg tcatgatatg gcgcgacgta gacctctttc
     1021 ttgctacggt cgataccatc catattcccc agacggaaat agaaatggtg ccagtgggct
     1081 tgcgccaggt actccagttc atgcttgtct ggattcatgc tgcctgcggc gatctcatgc
     1141 aacagaggtt tccagacatg ggtccggttg cggtcgatga gcgtgatatg tgctttgccc
     1201 ttccgctcga gggagtcccc caggcgcgtg gctagctcaa ggccgccggc accgccaccg
     1261 acgatgacga cgtgatgcaa agacgaattc gacgttgaat ccaaagacat aaactgattt
     1321 ttcctgatac tagaattggt tactcgttac ccgtggcgtt accgtggcta cggatccaag
     1381 cgatgatctt cagcacttcg tcggggctaa gtccgttggc tggatcggtt ggatccatca
     1441 ggcccttgcc ctttgcaccc atcccgccat tggtaccatg ccagatggtt tcgaacatcc
     1501 ctttgttggt c
//
LOCUS       DATMTW010000187         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_553504, whole genome shotgun sequence.
ACCESSION   DATMTW010000187 DATMTW010000000
VERSION     DATMTW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553504"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1849)
                     /locus_tag="VN084_03560"
     CDS             complement(<1..>1849)
                     /locus_tag="VN084_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HWT28566.1"
                     /translation="ELYWQLEEIAQDMEFKLNTEEAKYDQIHRALLSGLLGNIGFKDG
                     EADTYQGARGIRFSIAPGSCLKKTRPKWVMAAELMDTSKLYARCVAKIEPDWIEPLAR
                     GLTESHYSDPRWDRKPAQVIAWERVSLYGLTIVPKRRVHYGPIDPAESREIFIREALA
                     NMEFDTRAPFFAANKRLIAEIEELEHKARRQDVLVDEHVLFAFYDSRVAQGIVNGAGF
                     EKWREDAEKANPKLLYLTKDDLMRHGAAGVTEAQFPETFELDGITVPLKYRFEPGHVL
                     DGVTATIPLALLNQLDPTRAEWLVPGMLREKVTHLIKSLPKTIRRVCVPVPDFVTAFL
                     EDISKHPSFQRKLESSQSVEKYMDSGVRRNDSTESPPLLQTLAAFIQQRTGLKLGADD
                     WKEAVPDHLLMNFRVIDEQQRELGMGRHWNALKQQLGQAAQLTFRSATPGIEKSGLKQ
                     WDFGDLPKTLTFTRDGRQLTGYPALEDNGDSVSIKLFDTQSAADQAHRAGVRRLMRFE
                     LKEQMKQLEKGLPGFNQYALLLRNLFSPDDLREDMLTAIADRAFIGDDELPRSNDEFM
                     KLKVRARTRLPAVTEALVRQVQAIASEYQLLATKQSQMPATVNRLKRDVE"
BASE COUNT          301 a          552 c          603 g          393 t
ORIGIN      
        1 gctcgacatc gcgcttgagg cggttgacgg tagcaggcat ctggctctgc ttggtagcga
       61 gcagctggta ttcgctggcg atggcctgca cctgccgcac cagcgcttcg gtgaccgccg
      121 gcaaacgcgt gcgcgcccgc accttgagtt tcatgaattc gtcattgctg cgcggcagct
      181 cgtcatcacc gatgaaggca cggtcggcga tggcggtgag catgtcttcg cgcaggtcat
      241 ctgggctgaa caggttgcgc agcagcaagg catattggtt gaagcccggc aggcctttct
      301 ccagttgttt catctgctcc ttgagctcga aacgcatcag gcgccgcacc cctgcccggt
      361 gcgcctggtc ggcggcggac tgggtatcga acagtttgat ggagacgcta tcgccgttgt
      421 cttccagcgc ggggtagccc gtgagctggc ggccatcacg tgtgaaggtc agcgtcttgg
      481 gcagatcgcc gaaatcccat tgcttgaggc cgctcttttc gatgcccggc gtagcgctgc
      541 ggaaggtgag ctgcgcggcc tgccccagct gttgcttgag ggcgttccag tgacggccca
      601 tgccgagttc gcgctgctgc tcgtcgatga cgcggaagtt catcagcagg tggtcgggga
      661 cggcttcttt ccagtcatcc gccccaagtt ttaggccagt gcgttgctgg atgaaggcgg
      721 cgagcgtttg aagaagcggc ggcgattcgg tgctgtcatt ccggcgcacg ccggaatcca
      781 tatatttctc aaccgactga ctggattcca gctttcgctg gaatgacggg tgtttggaaa
      841 tatcttcgag gaacgccgtg acgaaatctg gtaccggcac gcacacgcgg cgaatggtct
      901 tgggcagcga cttgatgagg tgcgtgacct tctcgcgcag catacccggc accagccatt
      961 cggcgcgcgt ggggtcgagc tggttaagca aggcaagtgg gatggtggcc gtcacgccat
     1021 ccagcacatg gcccggctcg aagcgatact tgagcggcac ggtgatgccg tcgagctcga
     1081 aggtctccgg gaactgcgcc tccgtgacgc ccgccgcgcc gtggcgcatg aggtcgtcct
     1141 tggtcaggta gagcagtttg gggttggcct tctcggcgtc ttcgcgccat ttctcgaaac
     1201 ccgcaccgtt gacgatgccc tgcgcgacac ggctatcgta gaaggcgaac agcacatgct
     1261 cgtccaccag cacatcctgc cgccgtgcct tgtgttccag ctcctcgatc tcggcgatga
     1321 gacgcttgtt ggcggcgaag aacggcgcgc gcgtgtcgaa ttccatattg gccagcgctt
     1381 cgcggatgaa gatctcgcgc gactcggccg ggtcgatcgg cccgtaatgc acgcggcgct
     1441 tgggcacgat ggtgaggccg taaaggctga cgcgctccca ggcgatgacc tgcgccggtt
     1501 tgcgatccca gcgcgggtcg ctgtaatggc tctcggtcag cccgcgcgcc agcggttcga
     1561 tccagtccgg ctcgatcttg gcgacgcagc gcgcgtagag tttggaggta tccatcagct
     1621 ctgcagccat cacccacttg gggcgggtct tcttcaggca ggagccgggc gcgatgctga
     1681 agcggatgcc acgcgcgccc tggtaggtgt cggcttcgcc gtccttgaag ccgatgttgc
     1741 ccagcaaacc cgatagcaac gccctgtgga tctggtcata tttggcttct tcggtattga
     1801 gcttgaactc catgtcctgc gcgatctctt ccagctgcca gtagagttc
//
LOCUS       DATMTW010000188         5586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_16802, whole genome shotgun sequence.
ACCESSION   DATMTW010000188 DATMTW010000000
VERSION     DATMTW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16802"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VN084_03565"
     CDS             complement(<1..1325)
                     /locus_tag="VN084_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HWT28567.1"
                     /translation="MQAQSPEAQGKPDQQGLYNPANEHDACGVGFIAHIKGKKSHDIV
                     RNGLQILANLTHRGATGYDPKLGDGAGMLIQMPDAFMRKEAAKQGITLPEVGHYAVGM
                     VSLPQSRNGREACESVIARIVHEEAQTLLGWRDVPRDNSNIADAARAVEPVMRQVFIA
                     SAAADQNAFERKLFVIRKRIEHAVRALKIEDACQFYIPSLSSRTIVYKGMLLANEVGT
                     YYQDLQDETMVSALALVHQRFSTNTFPAWELAHPFRMIAHNGEINTMQGNVNWMAARH
                     AAMRSELLGEDLDKVWPLIADGQSDSACFDNALELLVAGGYSLPHAMMMLIPEAWSSD
                     PLMDEQRRAFYEYHAALMEPWDGPAAVAFTDGRMIGATLDRNGLRPARYLVTDDDHVM
                     MASEMGVLTFPQERIIKKWRLQPGKMLLIDMEQGRIIDDAEVKQQLAEA"
     gene            complement(1531..2817)
                     /gene="hemL"
                     /locus_tag="VN084_03570"
     CDS             complement(1531..2817)
                     /gene="hemL"
                     /locus_tag="VN084_03570"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWT28568.1"
                     /translation="MTSRNQQLFERSQRLIPGGVNSPVRAFRSVGGTPVFFKRGLGAH
                     LWDEDDKEYIDYIGSWGPMILGHAHPEVIQAVQAAAENSLSFGAPTALELEMAERIIE
                     LVPSMEQVRLVSSGTEATMSAIRLARGYTGRNKILKFEGCYHGHADSLLVKAGSGALT
                     FGQPSSAGVPPELAAHTLTLPYNDNAAVEQLFAQIGSEIACVIVEPVVGNMNLIVPQN
                     GFLQVLRDQCTRHGAVLIFDEVMTGFRVALGGAQQLYGITPDLTTLGKVIGGGLPVGA
                     FGGRHDIMHCLAPLGPVYQAGTLSGNPVAVSAGLATLKLIQVPGFHERLGAQTGKLVD
                     GLAAAAREAGVAFSAQHVGGMFGLYFSARCPTSYDEVMQSDKEAFNRFFHGMLDEGIY
                     LAPSAFEAGFVSAAHTDLDIEKTIAAAKKVFAGKLH"
     gene            complement(2844..3473)
                     /gene="thiE"
                     /locus_tag="VN084_03575"
     CDS             complement(2844..3473)
                     /gene="thiE"
                     /locus_tag="VN084_03575"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWT28569.1"
                     /translation="MKKMTRGRINGLYALTPDEPDTAKLLRLVEQALDAGVKLLQYRN
                     KQADSRLRIAQAQAILPRCHHHGATFIINDDIKLCLAIDADGVHLGKTDGSLGAARER
                     LGPDKLLGASCYDRFELAVAAKTHGADYVAFGACFPSATKPAAPRADLQLFTRAKSEL
                     GLPTVAIGGITPDNAGLAFSAGADAVAVIGALWNSADIRAIVQQFQHSQ"
     gene            complement(3445..4281)
                     /locus_tag="VN084_03580"
     CDS             complement(3445..4281)
                     /locus_tag="VN084_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HWT28570.1"
                     /translation="MNLMPPTVMTFAATDPSSGAGLQADILTLASIGCHPLSVVTAVT
                     VQDTVGVDNVMVCDADWVNDQARTILEDMQVSAFKLGMLGSVENIAVIAEIMADYPDT
                     PLLIDPVLASGRGDELANEEMQAAMIDLLLPQATLITPNSLEARRLAFHDDDSDEAEN
                     ASLEEVADRLLAMGSEYVLITGTHERTAEVINTLYGERGIIQTFKCERLPGSYHGSGC
                     TLTSAIAACVAHGLSIEEAIAEAQDYTWQSLKHAFRPGMGQFIPDRLFWARDEEDDAR
                     KN"
     gene            4422..4586
                     /locus_tag="VN084_03585"
     CDS             4422..4586
                     /locus_tag="VN084_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HWT28571.1"
                     /translation="MKTWMCNICGWIYDEAKGCPEDGIAPGTRWEDVPPNWTCPECGA
                     RKEDFEMVEI"
     gene            complement(4600..4673)
                     /locus_tag="VN084_03590"
     tRNA            complement(4600..4673)
                     /locus_tag="VN084_03590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4639..4641),aa:Gly,seq:ccc)
     gene            4876..5076
                     /locus_tag="VN084_03595"
     CDS             4876..5076
                     /locus_tag="VN084_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWT28572.1"
                     /translation="MNWDQIEGNWKQFKGKVHQQWGKLTDDQLEEIKGNRQMLSGKIQ
                     EMYGVTKEEAEEQIDRFNKACH"
     gene            complement(5170..>5586)
                     /locus_tag="VN084_03600"
     CDS             complement(5170..>5586)
                     /locus_tag="VN084_03600"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWT28573.1"
                     /translation="FQFTNAKGEQRYGRYRITPLAGDKALSETEASAAAPNYLSEELK
                     SRLAQGPAKFRIAVQLAEKGDVIDDATMVWPDSRPQIELGVLTLKSVADDSAAVEKKL
                     AFNPLILTDGIAPSNDPILMARPAAYAISVGRRFAD"
BASE COUNT         1098 a         1616 c         1748 g         1124 t
ORIGIN      
        1 ttggcttcag ccaactgctg cttgacttcg gcatcatcga tgatgcggcc ctgttccatg
       61 tcgatgagca gcatcttgcc cggctgcagg cgccatttct tgatgatgcg ttcctgcggg
      121 aaggtcagca cacccatctc ggaggccatc atgacgtggt catcatcggt caccaggtag
      181 cgggccggac gcaaaccgtt acggtccagc gtggcgccga tcatgcggcc atcggtgaag
      241 gctacggcag cagggccgtc ccagggctcc atcagagcgg catgatattc atagaaggcg
      301 cggcgctgct catccatcag cggatcggaa gaccaggctt ccgggatcag catcatcatg
      361 gcgtgcggca gcgagtagcc accggcgacc agcagttcga gcgcattgtc gaaacaggcg
      421 gaatcagact ggccgtcagc gatcagcggc caaaccttgt ccaggtcttc gcccagcagt
      481 tcggagcgca tcgccgcgtg gcgcgcggcc atccagttga cgttaccctg catggtgttg
      541 atctcgccat tgtgggcgat catgcggaag ggatgcgcca actcccatgc cgggaaggtg
      601 ttggtggaga aacgctggtg caccagcgcc agggcagaga ccatggtctc gtcctgcagg
      661 tcctggtaat aggtgccgac ctcgttggcc agcagcatgc ccttgtagac gatggtgcgg
      721 ctcgacagcg aagggatgta gaactgacag gcatcctcga tcttcagggc acgcaccgcg
      781 tgctcgatgc gcttgcggat gacgaacagc ttgcgttcga aggcattctg gtcggcggct
      841 gcgctggcaa tgaacacttg gcgcatgacc ggctcgactg cgcgtgcggc gtcagcgatg
      901 ttgctgttgt cgcgcgggac atcgcgccag cccagcaggg tttgcgcctc ttcgtgtacg
      961 attcgggcga tgacggattc acaggcttcg cgaccgttgc gcgactgcgg aagcgagacc
     1021 atgccgacgg cataatggcc gacctctggc aaggtgatgc cttgtttggc cgcttccttg
     1081 cgcatgaagg catcgggcat ctggatcaac atgccggcgc cgtcgccgag cttggggtcg
     1141 taaccggtcg cgccgcgatg cgtcaggttg gcaaggatct gcaagccgtt acgaacgatg
     1201 tcgtggcttt tcttgccctt gatatgcgcg atgaagccga cgccacaggc gtcatgttcg
     1261 ttggcagggt tatagagccc ctgttgatcg ggcttgcctt gtgcttctgg cgattgtgct
     1321 tgcattacca atgtatttac ctatccatcc ctcgacccct gtactgccgc gccaaccgag
     1381 ggtgttggcg gggtttcgaa catcgttctg gccaaattcg gcgcaggcca tgaaatattt
     1441 agcggaacct tcaatgttac cgtcaaaagg ttatgagttc aatcacatag acgcattttt
     1501 catgccgcaa gcccgtaaac ttgccgagtt tcagtgcagc ttgccggcaa acaccttttt
     1561 cgcggcggcg atcgtcttct cgatgtcgag atcggtatgt gcggcggaca cgaaacccgc
     1621 ttcaaaagcg gagggcgcca gatagatgcc ttcgtccagc atgccatgga agaagcgatt
     1681 gaaggcttcc ttgtccgact gcatgacttc gtcgtagctg gttgggcaac gcgcgctgaa
     1741 atagaggccg aacatgccgc cgacatgctg cgcgctgaag gccacaccgg cttcacgagc
     1801 cgctgccgcc aggccatcga ccagcttgcc ggtctgtgcg ccaagtcttt cgtggaaacc
     1861 cggcacctgg atcagcttga gcgtcgccag gccggcagac acggccaccg gattgccgga
     1921 cagcgtaccg gcctggtaca ccggccctag tggcgccagg caatgcatga tgtcatggcg
     1981 accgccgaaa gcaccgaccg gcaagccgcc gccgatgacc ttgcccagcg tagtgaggtc
     2041 cggggtgatg ccatagagct gttgcgcgcc gccgagtgcg acacggaacc cggtcatcac
     2101 ttcatcgaag atcaacaccg cgccatgcct ggtgcactgg tcgcgcaata cttgcaagaa
     2161 gccgttctgc gggacgatga ggttcatgtt gccgacgacc ggttcaacga tgacgcaggc
     2221 gatctcactg ccgatctgcg cgaacagctg ctcgaccgcg gcattgtcgt tgtaaggcag
     2281 cgtcaaggtg tgcgcggcga gttccggtgg cacgcctgcc gagcttggct ggccgaacgt
     2341 gagcgcgccg gaaccggctt tgaccagcag cgagtcggca tggccgtgat aacagccttc
     2401 gaatttcagg atcttgttgc ggccggtgta gccacgcgcc aggcggatgg cgctcatggt
     2461 ggcctcggta ccggaactga cgagccgtac ctgctccata ctcggtacga gctcgatgat
     2521 gcgttctgcc atctccagct cgagtgccgt cggcgcgccg aaggacaggc tgttctcggc
     2581 cgcagcctgc acggcttgaa tcacctcagg gtgcgcatga ccaaggatca tcggccccca
     2641 ggaaccgatg tagtcgatgt attccttgtc gtcctcgtcc cacaggtgcg cgccgaggcc
     2701 acgcttgaag aataccggcg tgccgccgac ggagcggaag gcacggaccg gtgagttgac
     2761 gccgcccggg atcaggcgct gggatcgttc gaagagctgc tggttacgtg aggtcatgat
     2821 ggccttgctg aaaaagttag tggctattgg gaatgctgaa actgctgaac gatcgcgcgg
     2881 atatcggcac tattccagag cgcgccaatg actgcaaccg catcggcgcc agcgctgaag
     2941 gccagccccg cgttgtcggg cgtgatgccg ccgatggcga cagtcggcaa gccgagctca
     3001 ctcttggcgc gcgtgaaaag ctgcaggtcg gcgcggggtg cggcaggctt ggtcgcggag
     3061 gggaagcagg cgccgaaggc gacgtaatcc gccccatgcg ttttggccgc gacggccagt
     3121 tcgaaacggt catagcacga agccccgaga agcttgtctg ggccaaggcg ttcgcgcgct
     3181 gcgcccagac tgccgtcggt cttgcccaga tgcacgccgt cagcatcgat ggcgagacag
     3241 agcttgatat cgtcattgat gatgaaggtc gcaccgtggt ggtggcagcg cggcaatatt
     3301 gcctgtgcct gcgcgatgcg caggcgggaa tcggcctgct tgttacggta ttgcagcagc
     3361 ttgacgccgg catccagcgc ctgctccacc agccgcaaca acttcgccgt atccggctcg
     3421 tccggcgtca gcgcgtaaag gccgttaatt cttccgcgcg tcatcttctt catccctcgc
     3481 ccagaacagg cgatccggga tgaactggcc catgcccgga cggaatgcgt gcttgagcga
     3541 ctgccaggta tagtcctgcg cttcggcgat ggcttcctcg atgctcaaac catgagcgac
     3601 acaggcggcg atggccgagg tcagcgtgca gccggagccg tgatagctgc cgggcaggcg
     3661 ctcgcacttg aatgtctgga tgatgccacg ctcgccgtac agggtattga tgacttccgc
     3721 ggtgcgctcg tgggtgccgg tgatcagcac atattccgaa cccatggcca gcaagcggtc
     3781 tgcgacttcc tccagcgagg cgttctcggc ttcgtcggaa tcgtcgtcgt gaaaagcgag
     3841 gcggcgcgcc tcgaggctat tgggcgtgat gagtgttgcc tgcggcaaga gcaggtcgat
     3901 catcgctgcc tgcatctctt cgttggccag ctcatcgcca cggccggagg ccagtaccgg
     3961 gtcgatgagc aggggggtat ccgggtaatc ggccatgatc tccgcgatca cggcgatgtt
     4021 ctcgacgctg cccagcatgc ctagcttgaa ggcggagacc tgcatgtcct ccaggatggt
     4081 gcgtgcctgg tcgttgaccc agtcagcatc gcataccatg acgttatcga cgcccacagt
     4141 gtcttgcacc gtgacggcgg tgacgaccga aagcggatga cagccgatac tggccagggt
     4201 caggatatcg gcctgcaaac cggcgccgct gctggggtcg gtcgcggcga acgtcatgac
     4261 ggtagggggc atcagattca taatgagatt ctcagcacga gacacttatg gcatgggcca
     4321 tgcgacttag ttcatggctg cgagtgctgt gggcgcgatg taatttggaa tatgctattt
     4381 taacgcaatt caccttctcg ataataaaag gtctctggca aatgaaaacc tggatgtgca
     4441 atatctgcgg ctggatctac gatgaagcga aaggttgtcc cgaggacggc atcgcacctg
     4501 gaacacgttg ggaagacgta cctccgaact ggacctgtcc ggaatgcggg gcgcgcaagg
     4561 aagatttcga gatggtggag atctgacgga ctgaagtagt ggagcgggcg acgggaatcg
     4621 aacccgcgta tcgagcttgg gaagctagcg ttctaccatt gaactacgcc cgcaatgccg
     4681 ccattctacg cagttcgcca ctcgactggc aaacctgccg cgtagtcttt gcatctgttg
     4741 tcagcccata cctacacctg attcagcctg atgccgactg tcctgcgcgt tcttgccgat
     4801 taagatgagc ccatggtcga tcgtgatcgc acgatacaga ccggacactc acctgatatc
     4861 gaaaaggagt acatgatgaa ctgggaccaa atcgaaggta actggaagca attcaagggc
     4921 aaggtacatc aacagtgggg caagctcacc gatgaccagc tcgaagagat caagggtaat
     4981 cgccagatgc tgtcgggcaa gatccaggag atgtatggcg tgaccaagga agaagcggaa
     5041 gaacagatcg accgcttcaa caaggcttgt cactgagcca cgctcaacgc tcagcgatat
     5101 tgctcaaatc tccatcgcga ttccaaaggc caccctcggg tggccttttt ttacgcgatt
     5161 gagtcgtatc tagtcggcga agcggcggcc gacactgatg gcataggccg caggccgtgc
     5221 catgaggatc ggatcgttgc tgggcgcgat gccatccgtc aggatcaggg gattgaacgc
     5281 cagcttcttt tccaccgcgg cggaatcgtc ggcgacggat ttcagcgtca ggacgcccag
     5341 ctcgatctgt gggcggctgt cgggccagac catcgtcgcg tcatcgatta catcgccttt
     5401 ctcagccagt tgtaccgcga tacggaattt ggccgggcct tgcgccaatc gtgacttcag
     5461 ttcctccgac aggtagttcg gtgcagccgc gctcgcttca gtctcgctca atgctttgtc
     5521 tcctgccagc ggcgtgatgc ggtaacgacc ataacgctgc tcgcccttgg cattggtgaa
     5581 ctggaa
//
LOCUS       DATMTW010000189         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_50926, whole genome shotgun sequence.
ACCESSION   DATMTW010000189 DATMTW010000000
VERSION     DATMTW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50926"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VN084_03605"
     CDS             complement(<1..1261)
                     /locus_tag="VN084_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWT28574.1"
                     /translation="MTEVVQKILTVDDDPDILKLLGMRLKAAGYQVVEAKSGEEALTQ
                     IAVSRPVLVISDLRMPGMDGMALFEAIQQTDPALPVIMLTAHGSIPEAVEATQRGVFG
                     FLTKPFDSKLILQQVEAALRISSGKNAHVRSKADEDWRRDIVTRSPQMENLLGQAKLM
                     AVSDASVFIQGESGTGKELLARAIHRASPRHDKPFIAINCGAIPEALLESELFGHSKG
                     AFTGALRDHKGLFQTADGGTLLLDEIGDMPMALQVKLLRALQERVIRPVGSSTSIPID
                     VRVISATHRNLAEEMKAGRFREDLYYRINVVGLEIPALSARREDIPLLANNFLSSLNE
                     KYHKKLNGFAPEAMELLISAPWPGNVRQLQNIVEQTVVLSTTPLIPASLVQKALQDDI
                     GGIVPFETARKNFEREYLVRLLKATNGS"
     gene            complement(1258..1752)
                     /locus_tag="VN084_03610"
     CDS             complement(1258..1752)
                     /locus_tag="VN084_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28575.1"
                     /translation="MNRPAIPFLFALCLLSACAQLPDSQSKVPVTQSPTISRLDMLDF
                     AESFSELSLEGQKKELQQNSQNRNDMTSRIKVALIYALPTSKLRDTNKAQALLDDLAK
                     DKYLDGDRKTLTLIMRDYIAEINRSGNKAREEQKRADALQQKLDELKNIEKSMLEREQ
                     GNRK"
     gene            complement(1745..>3148)
                     /locus_tag="VN084_03615"
     CDS             complement(1745..>3148)
                     /locus_tag="VN084_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28576.1"
                     /translation="LLIGFALAMLPLLFAFGNAAVYLDRLADKSRDTVTQATQTTRFS
                     RSLQEQIMLMERSARQYFVLNDALLLENYGNAHEKFTHALQELQALPMAKRQRDILDS
                     LENRERVLYDQVTTPLPESRSPQDIVSGFITLSGEAETLLAENNQMIDREAAVLAETA
                     GRTQKMLLWQTLTLVPVAFLVAIAITFLVAQPIKRMDLAIRRLGEGNYAEPISIDGPG
                     DLRTLGERLDWLRSQLDAVDKQKQLFLRHVSHELKTPLTAIREGSELLSDEIGGSLNQ
                     QQKEIAGILRESSLRLQKMIENLLSYSAVQFQKPHLKIERIQLDSLINDILSNYALTL
                     STKNIRIHREFNALQLFGDHEKVQTVIDNLISNAIKFTPASAAIQLRTYQEAENVFIE
                     VHDGGPGVLTADKARLFDPFYQGDSVYDSLVSGSGLGLSIAKEYADAHGGEIRLLPSD
                     HGAHFSVRLPFKAENHE"
BASE COUNT          553 a          872 c          989 g          734 t
ORIGIN      
        1 cgctgccgtt ggtagccttg agcaggcgta ccaggtattc gcgttcgaag ttcttgcgcg
       61 ccgtctcgaa ggggacgatc ccgccaatgt cgtcctgcaa agccttctgc acgagcgagg
      121 cggggatcag tggcgtggtc gatagcacca cggtctgttc gacgatgttc tgcagctggc
      181 gcacgttgcc cggccacggg gcgctgatga gcagctccat ggcttcaggc gcaaagccat
      241 tgagtttctt gtgatacttt tcattcagtg aactgaggaa attgttggca agcagcggga
      301 tgtcttcacg gcgtgccgac agggccggga tctcgagtcc gacgacgttg atgcggtaat
      361 acaggtcttc gcggaaccgg ccggccttca tttcttccgc aaggttgcgg tgagtcgccg
      421 agatgacgcg gacgtcgatg ggaatgctgg tgctggaacc caccggacga atgacgcgct
      481 cctgcagcgc gcgcagcagc ttgacctgga gcgccatcgg catgtcaccg atctcatcga
      541 gcagcagggt gccgccgtct gcggtctgga acagcccctt gtggtcgcgc agcgcgccgg
      601 tgaaggcgcc tttgctgtgg ccgaacagtt cggattcgag caatgcctca gggatggcgc
      661 cgcaattgat ggcgataaag ggcttgtcat gccttgggct ggctcgatgg atggcgcgcg
      721 ccagcagctc cttaccggtg ccgctctcac cctggatgaa gacgctggcg tcggaaaccg
      781 ccatcaactt ggcctggccc agcaggttct ccatctgtgg actgcgtgtg acgatgtcgc
      841 gtcgccagtc ctcatcggcc ttcgatctga catgcgcgtt cttgccactg ctgatgcgca
      901 gggcagcctc gacttgttgc agtatcagtt tgctgtcgaa cggcttggtc agaaaaccga
      961 agacgccgcg ctgggtcgct tccaccgctt cggggatcga gccgtgcgcg gtcagcatga
     1021 tgacgggcaa ggccgggtca gtctgttgga tggcctcgaa taaagccatg ccatccatgc
     1081 ccggcatacg caagtcgctg atcaccaaca ccggtcggct gacggcgatt tgtgtcaggg
     1141 cttcttcacc gctcttggct tccacgacct ggtagcccgc cgccttgaga cgcatgccaa
     1201 gcagcttgag gatgtccgga tcgtcgtcga ccgtcaggat tttttgtact acttcagtca
     1261 tttacggttc ccttgctcac gttcgagcat cgatttttcg atgttcttga gctcatcgag
     1321 tttctgttgc agggcatcgg cgcgcttctg ttcttcgcgc gccttgttgc ccgatcggtt
     1381 aatttcggcg atgtaatccc gcatgatcaa ggtcagtgtc ttgcggtcgc catcgaggta
     1441 cttgtcctta gccaagtcat caagcaaggc ttgtgccttg ttcgtatcgc gcagcttgct
     1501 ggtgggcagc gcatagatca gcgcgacctt gatacggctg gtcatgtcgt tcctgttttg
     1561 gctgttctgc tgcagttcct tcttttgacc ctcaaggctg agttcgctga aactttcggc
     1621 aaaatccagc atatcgagac ggctgatggt gggggattgc gtgacaggga ctttcgattg
     1681 cgaatccggc aattgggcgc acgcgctcaa caggcacaac gcgaacaaga aggggatggc
     1741 tggtctattc atggttttcc gctttgaatg gcaggcgcac actgaaatgt gcgccgtggt
     1801 ctgatgggag caggcgaatc tcgccgccgt gtgcgtctgc atattccttg gcgatggata
     1861 ggccgaggcc gctgccgctg accaggctgt cgtagacgct gtcgccctgg tagaacggat
     1921 cgaacaatcg cgccttgtcg gcggtgagta cgcccgggcc gccgtcgtgg acttcgatga
     1981 acacattctc ggcttcctgg taggtgcgca gctggatagc cgcggaagcc ggtgtgaatt
     2041 tgatggcgtt ggagatcaga ttgtcgatga cggtctgcac cttctcatga tcgccgaata
     2101 attgtaacgc attgaattct cggtggatcc gtatgttctt tgtgctcaag gtcagcgcgt
     2161 agttgctgag gatgtcgttg atcagactat ccagctggat acgttcgatc ttgaggtgtg
     2221 gcttctggaa ctgcaccgcg ctgtagctca atagattctc gatcatcttc tgcaggcgga
     2281 ggctgctttc gcgcaggatg ccggcgatct ccttctgctg ctggttgagc gaaccgccga
     2341 tctcgtcgct cagcagctca ctgccttcgc ggatggcggt cagtggggtc ttcagttcgt
     2401 gcgagacatg ccgcaggaac agctgcttct gcttatccac cgcatcgagc tgcgagcgta
     2461 gccagtcgag gcgttcgcct agcgtacgca ggtcgcccgg cccgtcgata ctgatcggtt
     2521 ccgcataatt gccttcaccg aggcggcgga tagcgagatc catgcgcttg atcggctgtg
     2581 ccacgaggaa agtgatggcg atggcgacca ggaaagctac gggtaccagg gtcaaggtct
     2641 gccacagcaa catcttctgg gtgcgcccgg cggtttccgc cagtaccgcg gcttcgcggt
     2701 cgatcatctg gttgttttcg gcgagcagcg tctcggcctc accggacaag gtgatgaagc
     2761 cgctgacgat atcctgcggc gagcgcgatt ccggcagcgg cgtggtcacc tggtcgtata
     2821 ggacccgttc gcggttttca aggctatcga ggatatcgcg ttggcgcttg gccatgggca
     2881 gcgcctgcag ttcctgcagt gcgtgcgtga acttctcatg ggcgttgccg tagttttcca
     2941 gtagcaaggc gtcgttcagt acgaaatact gacgtgcgct acgttccatg agcatgatct
     3001 gctcctgcag cgagcgactg aaacgcgtgg tctgggttgc ctgggtcacg gtgtcgcggc
     3061 tcttgtccgc gaggcggtca agatagacgg cggcattacc gaaggcgaac aacagcggca
     3121 gcatcgccag ggcgaagccg atcaaaag
//
LOCUS       DATMTW010000190         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_419385, whole genome shotgun sequence.
ACCESSION   DATMTW010000190 DATMTW010000000
VERSION     DATMTW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419385"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1715)
                     /locus_tag="VN084_03620"
     CDS             complement(204..1715)
                     /locus_tag="VN084_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28577.1"
                     /translation="MRLIHSRALAVATGALLALAGCSQDLNIPDTNAPDTKRALSTPS
                     DVAALAASSIQTWQDASTEMNPNESVAVMADALTSSFNNFGMNRSAAEPRVAYPNNTT
                     GGDYTSMVQDPWYNNYTALGEANDVLSALGKGIVIDDAETTEAYKALAMLTQGLALGN
                     ISLFFDQGFVVDETAVLPGDPASMVKHGDVAAGALAKLDALIALTQGKTWTLPNDVLP
                     IPGVDLSAENIGKIANTMAARIVAYGARNATENAATDWNKVLTYAENGISSGTPFDFS
                     IQEDGGNVWWDGGKAYGEYGPWMRVSMRVIHEMNPAQPVEYTECHSQAEDAAGFTDAR
                     YQTDFTYIPTIPFQCARGAWHFSNWEHSRWGAKSVTADVSWYDGSFVGVAPLVLAAEN
                     DLLWAEALVRTGGDLGMAASLINKTRVGRGHLTPATAADGAAGLLADIQYEQDVELLD
                     TGGGLQWFNRRRIDGLQPGTPRHFPVPAKELEIDRLPTYTFGGAAPNPVYPDQ"
     gene            complement(1749..>3327)
                     /locus_tag="VN084_03625"
     CDS             complement(1749..>3327)
                     /locus_tag="VN084_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28578.1"
                     /translation="RDHANNAAVNATLSSSLSSLKSTTKLTYLYDDEREDVVNTNGNQ
                     FKLRNIQTLNAVDPTQFTANSAILNTRSLQYLVNQGLNWKDTYLAQATYVHDQSSLFG
                     SAARNNDFYQVSGAWRVTQNFHIPGFQELKLRAANGTAGLRPGFAYQYETYGVNNGQY
                     SKNTLGNNDLEPAIQHETEYGIDASFLNRFDLSVVRSQRKTNNAFLQVPLSIALDGGF
                     QYQWRNAATITGKTWELALTTRVIEQPNFSYNFTLTGEQTRQHIDRLDAPPFQQNAGG
                     QGQGIFYYRQGEDLGIIYGNKFVTDYSQLLDNPANAGMTLADIQALYTKNADGYVVLA
                     ANKGTPAEAPIVYVDKTGNNKVVIGNVNPDYTFGWANTIRVKNFTIYALINGTHGGNV
                     YNFTKQWMFFDLRSGDEDQSHRKQSDKIAYDYYANGIYNVLNPDSYFVEDGSFVKLRE
                     LSVNYQFSNSMLRQIGLGNFVRSLKVAVIGRNLKTWTKYSGFDPEAGSGGDANFRIDG
                     FRYPAFRTISGQIEIGF"
BASE COUNT          622 a          966 c         1009 g          730 t
ORIGIN      
        1 catctcgaca tttttggcgg gcactcggcg cggcttaccg aagtcgtggc gtgcgcgaat
       61 ttgtcgtaca agcagaagtg aagcgccgga cggcgtgacg ccggttgcca gccatgtgac
      121 tcctggcagc ggttgaaaac tcacggcccc ccggcgaatc attcgccggg gggccgtgtg
      181 aatttctgct acgtcgccat tgcttattgg tcaggatata ccggattcgg agcggcgccg
      241 ccgaaggtgt aagtcggcag ccggtcaatc tcgagctcct ttgcaggcac cgggaagtgc
      301 cgcggcgtac ccggctgcag cccatcaatg cgacgacggt tgaaccactg cagtccgccg
      361 ccggtatcca gcaattcaac atcctgctca tactggatgt ccgcaagcag acctgcagcg
      421 ccgtccgcgg cggtagccgg agtcagatgt ccccgtccta cgcgtgtctt gttgatgagc
      481 gaggccgcca tgcccagatc tccgcctgtg cgcaccagtg cttccgccca gagcagatcg
      541 ttttcggcgg cgagcaccag cggcgcgacg cccacgaagc tgccgtcgta ccagctcaca
      601 tcggctgtca cggacttggc gccccagcgt gagtgctccc agttgctgaa atgccatgca
      661 ccacgggcgc actgaaacgg gatcgtggga atataggtga aatcggtttg ataccgcgcg
      721 tccgtaaagc ccgcggcatc ttcagcctgt gaatggcact cggtatactc gacaggctgc
      781 gcggggttca tctcatggat gactcgcatg ctgacacgca tccatgggcc gtactcgcca
      841 tacgccttgc cgccatccca ccagacattt ccgccatctt cctggatgga gaagtcaaag
      901 ggagttcctg agcttatgcc attttcggca taggtcagca ccttgttcca atcggttgcg
      961 gcgttttcgg tcgcatttct ggcaccgtag gctacaatgc gcgccgccat ggtgttggcg
     1021 attttgccaa tattctctgc cgagagatca acgcccggaa ttggcagtac atcattcggg
     1081 agtgtccacg tcttcccctg cgtcagggcg atcagggcat cgagcttggc aagcgctccg
     1141 gccgcaacat cgccatgctt caccatcgaa gcgggatctc ccggaagcac cgctgtctcg
     1201 tcaacgacga agccctgatc gaaaaagagc gaaatgttgc ccagcgccag accctgggtc
     1261 agcatggcca gcgccttgta cgcctccgtg gtctccgcat cgtcgatcac gatgcccttg
     1321 ccaagggcgg aaaggacatc gtttgcttcg cccagagcag tgtagttgtt gtaccacggg
     1381 tcctgcacca tcgaggtgta gtcaccgccc gtggtgttgt tggggtaggc gaccctcggc
     1441 tccgcggcgc tgcgattcat gccgaagtta ttgaacgatg atgtcagcgc gtccgccatg
     1501 accgccaccg actcgttcgg attcatctcc gtgctggcat cctgccacgt ctggatggag
     1561 gacgcagcca gcgccgccac atcggacggc gtggacaacg cgcgcttggt gtcaggcgcg
     1621 ttcgtgtcag gaatattgag gtcctgtgag cagccggcca gagccagaag cgctccggtc
     1681 gctactgcca gtgccctcga gtggatcagt cgcataatgt gaatagctcc agtgtcgtat
     1741 gggcagggtc agaagccgat ttcaatctga cctgagatcg tgcggaacgc cggatagcgg
     1801 aatccgtcga tacggaagtt cgcatcgccg ccggagccgg cttccggatc gaatccgctg
     1861 tacttggtcc aggtcttcag gttgcggcca atcacggcaa ccttgaggct gcggacgaag
     1921 ttcccgaggc caatctgcct gagcatcgag ttggagaact ggtagttcac cgaaagctca
     1981 cgcagcttga cgaagctgcc gtcctccacg aagtagctgt cggggttcag cacgttgtag
     2041 atcccgttcg cgtagtaatc gtacgcgatc ttgtccgact gcttgcggtg cgactgatcc
     2101 tcgtcacccg agcggaggtc aaagaacatc cattgcttgg tgaagttgta gacgttgccg
     2161 ccatgcgtcc cattgatcag cgcgtagatc gtgaagttct tgaccctgat ggtgttggcc
     2221 cagccgaagg tgtagtcggg gttcacgttt ccgatcacga ccttgttgtt accagtcttg
     2281 tcgacataca cgatcggcgc ttcggcgggc gtacccttgt tcgccgccag cacgacgtat
     2341 ccgtcggcat tcttggtata gagcgcctga atatcggcga gcgtcatgcc ggcatttgcc
     2401 ggattatcga gcaattggct gtagtccgtg acgaacttgt tgccgtaaat gatcccgaga
     2461 tcttccccct gcctgtaata gaagatcccc tgcccctgac cgccggcgtt ctgctggaac
     2521 ggtggggcat cgagccgatc aatgtgctgg cgcgtttgct caccggtgag cgtgaagttg
     2581 tagctgaagt tcggctgttc aatcacgcgt gtcgtgaggg ccagctccca ggtcttgccc
     2641 gtgatggtcg cggcgttgcg ccactgatac tggaagccgc cgtcgagcgc gatggaaagc
     2701 ggaacctgga ggaaggcgtt gttcgtcttg cgctgagagc gaactacgct gagatcaaag
     2761 cgattcagga agctcgcgtc gatcccatac tcggtctcgt gctgaatggc tggctcgagg
     2821 tcattgttgc ccagcgtgtt cttgctgtac tggccattgt tcacaccata cgtctcgtac
     2881 tggtacgcaa acccgggacg tagaccagct gtcccattgg cggcgcgcag cttcaactcc
     2941 tggaatcccg ggatgtggaa gttctgcgtc acgcgccatg caccggatac ctgatagaag
     3001 tcgttgttcc gcgcagcgct gccgaacagc gaggactggt cgtgcacgta cgtagcctga
     3061 gccagatacg tatccttcca attcaggccc tggttgacga ggtactgcag cgaacgggta
     3121 ttgaggatcg ccgagttggc cgtgaactgg gttgggtcaa cggcattcaa cgtctgaatg
     3181 ttgcggagct tgaactgatt gccgttggtg ttgaccacgt cctcacgctc gtcgtcgtag
     3241 agataggtga gtttcgtggt cgacttgagc gatgagaggc tggagctcag cgtcgcattg
     3301 accgcggcgt tgttggcgtg atcgcga
//
LOCUS       DATMTW010000191         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_385641, whole genome shotgun sequence.
ACCESSION   DATMTW010000191 DATMTW010000000
VERSION     DATMTW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385641"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VN084_03630"
     CDS             <1..387
                     /locus_tag="VN084_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28579.1"
                     /translation="NLSLTGASEELHSAFGNLISNAIRYTPEGGDIVIRWEQRGQEAV
                     FSVKDTGIGIEQQHIDRLTERFYRVDRSRSRETGGTGLGLSIVKHILTRHQARLEIES
                     VPGQGSTFRVVFPKARIVLKQSALFK"
     gene            626..2653
                     /locus_tag="VN084_03635"
     CDS             626..2653
                     /locus_tag="VN084_03635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWT28580.1"
                     /translation="MSAGLMLAIGSAILAVLYGAIMSKWILSLPAGNARMQEIAKAVQ
                     EGAAAYLARQYKTIAIVGVILAILIGYFLGVTTAIGFIVGAVLSGACGFIGMNVSVRA
                     NVRTAQAATQGIAPALDVAFKGGAITGMLVVGLGLLGVTGFYMYLGGAEAQDLNPLIG
                     LAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGD
                     CAGMAADLFETYAVTIIATMVLGGLLLADAGTHGVLYPLMLGAVSIVASIIGCFFVKA
                     KPTMKNVMPALYKGLAVAGVLSLIAFYFVTGYLFPEGVTAGDMHVSKNALFGACAVGL
                     ALTAALVWITEYYTGTDYAPVKHVAQASTTGHATNIIAGIGISMKSTAWPVISVCIAI
                     FASYALAGLYGVAIAATAMLSMAGIVVALDAYGPITDNAGGIAEMADLPSSVRDVTDP
                     LDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLQGAGIDVKFDLSDPMVIIGLFIG
                     GLIPFLFAAMAMEAVGRAASSVVEEVRRQFRDIKGIMEGTAKPEYGKAVDMLTTAAIK
                     EMMVPSLLPVAVPIIVGLALGPVALGGLLMGTIVTGLFVAISMCTGGGAWDNAKKYIE
                     DGNHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPLL"
     gene            complement(2746..3294)
                     /gene="ppa"
                     /locus_tag="VN084_03640"
     CDS             complement(2746..3294)
                     /gene="ppa"
                     /locus_tag="VN084_03640"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWT28581.1"
                     /translation="MSLNQVPSGNALPDDFNVIIEIPMQGDPVKYEVDKESGAIFVDR
                     FMGTSMQYPTNYGYIPHTLSEDGDPVDVLVLTPVPLLPGVVVRCRPLGVLLMEDEAGL
                     DAKVLAVPVPKVCGLYGKFQSYKDVSEWRLNMIAHFFEHYKDLDQGKWVKINGWGDID
                     AARAEITSGVERYNEAEVKPAF"
     gene            complement(3370..4005)
                     /locus_tag="VN084_03645"
     CDS             complement(3370..4005)
                     /locus_tag="VN084_03645"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWT28582.1"
                     /translation="MSEFDKLINGYRRFHTNYFSAAKDELFAELSQGQSPSTLVIACS
                     DSRVDPAIVMDCKPGDLFVVRNVANLVPPYEKGGGYHGVSAALEFGVCALEVQHIIVL
                     GHRQCGGIKALFEGIPEGLAGEFIKPWVNMAKRAADRVRADYPHAADSEQLCECEMGG
                     IIVSLENLQTFPWIKERVAEGKLKLHGWYFDIVNGEMHAYDAQTLKFAPLA"
     gene            complement(4050..>4256)
                     /locus_tag="VN084_03650"
     CDS             complement(4050..>4256)
                     /locus_tag="VN084_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HWT28583.1"
                     /translation="QVKTELAVYKLVLRHARTPRVAKWLLWLAIAYLLLPFDLIPDFI
                     PVLGQLDDVLIVPGLVYLALIMIP"
BASE COUNT          847 a         1311 c         1221 g          877 t
ORIGIN      
        1 aacctcagcc tcaccggcgc cagcgaagag ctgcacagtg ccttcggcaa cctcatcagc
       61 aacgccatcc gctacacacc ggaaggtggc gatatcgtca tccgctggga gcagcgcggc
      121 caggaagcgg tgttcagcgt caaggatacc ggcatcggca tcgagcaaca gcacatagac
      181 cgcctcaccg aacgcttcta tcgcgtcgat cgcagccgat cgcgcgagac tggcggcacc
      241 ggcctcggac tctccatcgt caagcacatc ctgacccggc atcaagcccg cctcgaaatc
      301 gagagcgtgc cgggccaggg cagcaccttc cgcgtggtct tccccaaggc ccgcatcgtc
      361 ctcaagcaaa gcgcgctgtt caagtaatcc ggccttatcc tgcgtgacgc tgccagcgtt
      421 gtcgctacga gttaaagatg gaataatgga ttcgctgtaa attggtttgg gttggactgt
      481 tcgatcgttt cagatacgca agtaaataag aaggaaccga acggcgtcgg cagcatgcct
      541 gccagccaag ctctactgca atcacgtcac acgctttagc aacgcctgcg gttcccgttc
      601 actttttgag ataagggaga agtcgatgtc tgctggactg atgttagcaa taggtagtgc
      661 catccttgcc gtgctctacg gcgccatcat gagtaaatgg atattgtcgt tgccagccgg
      721 caacgcgcgc atgcaggaga tcgccaaggc cgtgcaggaa ggcgccgccg cctatcttgc
      781 gcgtcagtac aagaccatcg ccatcgtcgg tgtcatcctc gccatcctca tcggctattt
      841 ccttggggtc actaccgcca tcggcttcat cgtcggcgcc gtactctccg gtgcctgcgg
      901 cttcatcggc atgaatgtct cggtccgcgc caatgtgcgt accgcgcagg ccgccacgca
      961 aggcatcgct cccgcgctcg acgtcgcctt caagggcggc gccatcaccg gcatgctggt
     1021 ggtcggctta ggcctgctcg gcgtcaccgg tttctatatg tatcttggcg gcgccgaggc
     1081 acaggacctc aatccgctga tcggcctcgc cttcggttcg tcgctgatct cgatcttcgc
     1141 gcgcttgggc ggcggcatct tcaccaaggg tgccgacgtc ggcgccgacc ttgtaggcaa
     1201 ggtcgaggct ggcatcccgg aagatgatcc acgcaatccg gccgtgatcg ccgataacgt
     1261 cggcgacaat gtcggcgatt gtgccggcat ggccgccgat ctgttcgaga cttatgccgt
     1321 caccatcatc gccaccatgg tgctgggtgg actgttgctg gcggatgccg gcacccacgg
     1381 cgtgctctac ccgctgatgc tgggcgcggt ctccattgtc gcctccatca tcggttgctt
     1441 cttcgtcaag gccaagccga ccatgaagaa cgtcatgcca gccttataca aggggttggc
     1501 tgtcgctggc gtcctgtcct tgatcgcctt ctacttcgtc accggttacc tgttccccga
     1561 gggtgtcacc gccggcgaca tgcacgtcag caagaatgcg ctgttcggtg cctgtgccgt
     1621 tggcctggcg ttgactgccg cactggtgtg gatcaccgag tactacaccg gcaccgacta
     1681 cgcgccggtc aagcatgtgg cgcaggcttc cactaccggc catgccacca acatcatcgc
     1741 cggcatcggc atctccatga aatcgaccgc ctggccagtg atctcggtct gtatcgcgat
     1801 cttcgcctcg tatgcgctgg ctggcctcta tggcgtggcg atcgccgcca ccgccatgct
     1861 gagcatggcc ggtatcgtcg tcgcgctgga cgcctatggc ccgatcaccg ataacgctgg
     1921 tggcatcgcc gaaatggcgg acttgccaag cagcgtccgt gacgtgaccg acccactgga
     1981 cgctgtcggc aataccacca aggcggtgac caaaggctat gccatcggct cggcaggcct
     2041 ggctgcgctg gtgctgttcg ccgattacac ccacaaactg cagggtgcag gcatcgacgt
     2101 taaattcgac ctcagcgacc cgatggtcat catcggtctc ttcatcggcg gcttgatccc
     2161 gttcctgttc gccgccatgg cgatggaagc ggtaggacgc gccgctagtt cggtggttga
     2221 agaggtacgc cgccagttcc gtgacatcaa gggcatcatg gaaggcacgg caaaaccgga
     2281 atacggcaag gcagtcgata tgctgaccac tgccgccatc aaggagatga tggtgccttc
     2341 gctgctaccg gtggccgtac ctatcatcgt cggcctggca ctgggccctg tcgcgctcgg
     2401 cggcctgctg atggggacca tcgttaccgg cctcttcgtc gccatctcca tgtgtaccgg
     2461 tggcggtgcc tgggataacg ccaagaaata tatcgaggac ggcaatcatg gcggcaaggg
     2521 ttccgaggct cataaggccg ccgtcacagg cgacacggtc ggcgacccct ataaggatac
     2581 cgccggcccc gccgtcaatc cgctgatcaa gatcatcaac atcgtggcgc tgctgatcgt
     2641 gccgttgctg tgatgcttca tacgatcatc tgcgactgat cgcgcaacat aaaaaggcga
     2701 cctagaggtc gcctttttta tggtctggcc atcaacatca cggagctaga acgcaggctt
     2761 cacctctgcc tcgttgtagc gctcgacgcc ggaagtgatc tctgcgcgtg ccgcatcgat
     2821 atcaccccag ccgttgatct tgacccactt gccctgatcg aggtctttat agtgctcgaa
     2881 gaaatgcgcg atcatgttga ggcgccattc ggagacatcc ttgtagctct ggaacttgcc
     2941 atagagaccg cagacctttg gtacaggcac ggccagtacc ttggcgtcga gtccggcttc
     3001 atcttccatc agcaggacac ccaacggacg gcagcggaca actacgcccg gcaacagggg
     3061 aactggcgtc agcaccagga catcgaccgg atcaccgtct tcggacaagg tgtgcgggat
     3121 ataaccatag ttggtcgggt actgcatgga agtgcccatg aagcgatcaa cgaagatcgc
     3181 gccactttcc ttgtccactt catacttgac cggatcgccc tgcatgggga tctcgatgat
     3241 gacgttgaaa tcgtccggga gcgcgttacc ggaaggtacc tgattaagcg acataaaccg
     3301 tatttcctga aaaattcgac attatatgcc aagcagagcg gcaaacccac acgcgctctg
     3361 catccaggct caagccagtg gggcgaactt cagcgtctgc gcgtcatagg cgtgcatctc
     3421 gccgttgacg atatcgaaat accagccgtg cagcttgagc ttgccctcgg cgacacgctc
     3481 cttgatccag gggaaggttt gcaggttctc gagcgaaacg atgataccgc ccatctcgca
     3541 ttcacacagc tgctcgctgt cagcggcgtg tgggtaatct gcccgaacgc ggtcagccgc
     3601 gcgtttggcc atattgaccc aaggcttgat gaattcgcct gcgaggcctt ccgggatccc
     3661 ctcgaacaag gccttgatgc cgccgcactg acggtggccg agtacgatga tgtgctggac
     3721 ttccagtgca cagacaccga attcgagcgc ggcactgacg ccatgatagc cgccgccctt
     3781 ttcgtaaggc ggcacgaggt tagcgacgtt gcggaccacg aacaggtcgc ctggcttgca
     3841 gtccatgacg atcgccggat cgacgcggga atcggaacag gcgatgacca gcgtactggg
     3901 gctctgtccc tggctcagct cggcaaacag ctcatccttg gctgccgaga agtagttggt
     3961 gtgaaaacgg cggtaaccgt tgatgagttt gtcgaattcg ctcatgtttc tctctttgtt
     4021 atgcattgac gacactcgtc gatgacgtcc tatgggatca tgatgagcgc tagataaacc
     4081 agccccggca cgatcagcac atcgtccagc tgccccaata ccgggatgaa atccgggatc
     4141 aggtcgaatg gcagcagtag gtaagcgatc gcgagccaga gcaaccattt ggccacgcgc
     4201 ggggtacgcg catgacgcaa caccaacttg taaaccgcaa gctctgtctt gacctg
//
LOCUS       DATMTW010000192         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_16938, whole genome shotgun sequence.
ACCESSION   DATMTW010000192 DATMTW010000000
VERSION     DATMTW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16938"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VN084_03655"
     CDS             <1..424
                     /locus_tag="VN084_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWT28584.1"
                     /translation="AEIYKSIDANGHVTYSNMPSKGAKKLELEPPANTGFDSKPRERS
                     ETPSNFPRVDKQTQVQRDLKRKKILEDELSSEKKALEEAKKTYGENQTNAEFFRSNNL
                     KEPANIIKYDENLKHLEAEVESHKRNVELLQKELDSLK"
     gene            467..1549
                     /gene="glnL"
                     /locus_tag="VN084_03660"
     CDS             467..1549
                     /gene="glnL"
                     /locus_tag="VN084_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HWT28585.1"
                     /translation="MVQPIPTSYAGLEHLATAVILLDKSRRVVYANPGAEVLFAVSAK
                     QITGQNISQVFPGSEILHSAVENAIVNHSPFREHEFLITTMRQHSLAVTCTVTPADLA
                     PACLVLEFQQMDQQLRIAREERMLIQQQANAELLRNLAHEIRNPLGGLRGAAQLLEHE
                     LPQVNLREYTQVIIKEADRLQSLMDRLLVPHRVPKYEPTNVHEVLERVRSLLLAESPK
                     GVMVRRDYDTSLPELIGDREKLIQAVLNIARNAVQAMQGQGQITLRTRAERQVTLAKK
                     RYRVAIKIQIIDNGPGIPAEIRDRIFYPLVSGREGGSGLGLTLAQTFITQHHGMIECE
                     SQAGHTCFTIMLPIETTAMKCSISKQ"
     gene            1578..2966
                     /gene="ntrC"
                     /locus_tag="VN084_03665"
     CDS             1578..2966
                     /gene="ntrC"
                     /locus_tag="VN084_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HWT28586.1"
                     /translation="MKPIWIIDDDRSIRWVFEKALARTDMEFKTFSSVPEALNALDRE
                     EPQVVVSDIRMPNGSGLDFLEEIKKRLPDIPVIIMTAYSDLESAVAAFQGGAFEYLAK
                     PFDVDQAIEIIRRAVDESMRQATEAVSTEETPEIIGQAPAMQEVFRAIGRLSRSHATV
                     LINGESGTGKELVASALHRHSPRAEKPFIAINTAAIPKDLLESELFGHERGAFTGAQA
                     ARRGRFEQADGGTLFLDEIGDMPSDLQTRLLRVLCDGQFYRVGGHQPVKVNVRIIAAT
                     HQDLEERVKLGLFREDLFHRLNVIRLKLPPLRERREDVPLLVKHFLQQSAQELGVEPK
                     QPSAAALRYLSTMNWSGNVRQLENVCHWLTVMAPGQNIDVADLPPELKEDSAKPLSAA
                     SWQEALAAEVMDALNRGEQNVLETRTREFEKILITRALQHTGGRRIEAATQLGMGRNT
                     LTRKIQELGIEE"
BASE COUNT          710 a          926 c          814 g          540 t
ORIGIN      
        1 ggccgagatc tacaaaagta tcgacgccaa cggccatgtc acgtattcca acatgccctc
       61 aaaaggtgca aagaaactcg aactcgaacc accagcgaac accggcttcg acagcaagcc
      121 gcgcgaacgc agcgaaacgc cctcgaactt tccgcgcgtc gataagcaga cccaggtgca
      181 acgtgacctg aagcgcaaga agatccttga agacgaatta tccagtgaaa agaaggcgct
      241 cgaagaggcc aagaagacct atggcgagaa tcagaccaat gccgaattct tccgctcgaa
      301 caatctcaag gagcccgcca acatcatcaa atacgatgag aatctcaagc acctggaagc
      361 cgaagtcgaa tcgcacaagc gaaatgtcga actactgcaa aaagagctcg actccctcaa
      421 ataagcgcag cggcgctctg gcctagcttt tgcttatcag agagccatgg tacagcctat
      481 ccccacttcc tacgccggtc tcgaacatct cgccaccgcg gtcatccttt tagataaatc
      541 ccgcagagtc gtctacgcca atcccggcgc agaggtcctg ttcgcggtca gcgccaaaca
      601 gatcaccggc cagaacatct cgcaggtctt ccccggctcc gagatactgc attcggcggt
      661 ggaaaacgcc atcgtcaatc atagtccgtt ccgcgaacat gaattcctca tcacgaccat
      721 gcgtcagcac tctctggccg tgacctgcac cgtgacgccg gctgatcttg cccctgcatg
      781 cctggtgctt gaattccagc agatggacca acaattgcgt atcgcacgcg aagaacgcat
      841 gctcatccag cagcaggcca atgccgagct gctgcgtaac cttgcacacg agatccgcaa
      901 tccgctaggg ggcttgcgcg gtgctgcgca gctgctcgaa cacgagttgc cgcaggtcaa
      961 cctgcgcgaa tatacccaag tcatcatcaa ggaagcggat cgcctgcaat cactgatgga
     1021 tcgattactg gtgccgcacc gcgtgccgaa atacgaaccg accaatgtgc atgaagtgct
     1081 ggagcgtgta cgtagcctgc tgctggctga gtcgccaaag ggtgtgatgg tgcggcgcga
     1141 ctatgacacc agcctgcctg agctcatcgg cgatcgcgaa aaactgatcc aggcggtgct
     1201 caatatcgcc cgtaacgccg tacaggccat gcaaggccag ggccagatca ctttgcgtac
     1261 gcgcgccgaa cgccaggtga cgctagccaa gaagcgctac cgcgtcgcca tcaagatcca
     1321 gatcatcgac aacggccccg gcatccccgc cgagatccgt gaccgcatct tctaccccct
     1381 ggtatccggc cgtgagggcg gctcaggtct gggcctgaca cttgcacaga ccttcatcac
     1441 gcagcatcac ggcatgatcg agtgcgagag ccaggctgga cacacgtgct ttaccatcat
     1501 gctgccgatc gagaccacgg ccatgaaatg ctcgatcagc aagcaatgat ctcttaaacc
     1561 gcatcacagg caaaaccatg aaaccaatct ggatcataga cgacgatcgc tccatacgct
     1621 gggtgttcga aaaggctctc gcgcgcaccg acatggagtt caagaccttt tcatcggtac
     1681 cggaagcgct caacgcgctg gaccgtgaag aaccacaagt agtggtcagt gacatccgca
     1741 tgccaaacgg ctcaggcctc gactttctgg aagagatcaa gaagcgattg ccggatatcc
     1801 cggtcatcat catgaccgcc tattccgacc tggagagcgc ggttgctgca ttccagggcg
     1861 gggctttcga atatcttgca aaaccgttcg atgtcgacca ggccatcgag atcatacgcc
     1921 gcgccgttga cgagagcatg cgccaggcga ccgaggccgt ctcgaccgaa gagacgccag
     1981 agatcatcgg ccaggcaccg gcgatgcagg aagtctttcg cgccatcggc cgcttgagtc
     2041 gttcgcatgc taccgtactc atcaacggtg aatccggcac gggcaaggag ctcgtcgcca
     2101 gcgctttgca tcggcatagt ccgcgcgctg agaagccttt catcgccatc aacaccgcgg
     2161 ccatccccaa ggatctgctc gaatccgaac tgttcggcca tgaacgcggc gccttcaccg
     2221 gagcccaggc agcgcgacgc ggacgcttcg agcaggcaga tggcggcaca ttgtttctcg
     2281 atgagatcgg cgacatgcct tccgacctgc agacgcgctt actgcgtgtg ctttgcgacg
     2341 gtcagttcta tcgtgtcggc ggccaccaac cggtcaaggt caatgtgcgc atcatcgccg
     2401 ccacccacca ggatctggaa gaacgcgtca aactagggct gttccgcgaa gacctgttcc
     2461 atcgcttgaa cgtcatccgg ctcaagttac cgcctttgcg cgagcgccgg gaggatgtgc
     2521 cattactggt caagcacttc ctgcaacaga gcgcgcagga actgggcgtc gaacccaagc
     2581 agccttcggc cgcggccttg cgctatcttt cgaccatgaa ctggagcggc aatgtacgtc
     2641 agctggagaa tgtctgtcac tggctgaccg tgatggcacc aggtcagaac atcgatgtcg
     2701 ccgatctgcc gccagagctg aaggaggatt cagccaagcc ccttagcgcc gcttcttggc
     2761 aggaggccct ggcagcagaa gtcatggatg ccttgaatcg tggtgagcaa aatgtgcttg
     2821 agacccgcac ccgcgagttc gagaagattc tcatcacacg cgcgttgcag cacactggtg
     2881 ggcgccgtat cgaagcggcc acgcaactcg gcatgggccg taacacgctg acacgcaaga
     2941 tccaggagct ggggatcgag gagtgacccc agtctgacct agactgtagg
//
LOCUS       DATMTW010000193         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_318594, whole genome shotgun sequence.
ACCESSION   DATMTW010000193 DATMTW010000000
VERSION     DATMTW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318594"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VN084_03670"
     CDS             complement(<1..1080)
                     /locus_tag="VN084_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWT28587.1"
                     /translation="MRNWLLPEYLEDVLPAEAERIEQLRRRLLDLFKVHGYQYVIPPM
                     LEYLESLITGVGHDLDLATFKVVDQLTGRLMGVRADITPQTARIDAHMLNHQGVSRLC
                     YAGSVLRTNPDGLAQTREPLQVGAELFGHAGVESDIEIQRLMVKALQAIGIQSLHIDF
                     SHVGLFGNLIRQNRISDQLEQALYGALQSKDQSAVRVLASDLDPVTRDALCSLTELNG
                     GLEVIEVARQRLPHTAEIAKALDDLAQVGERLLDLGVDPCFDLSELRGYHYHSGIVFA
                     AYAQGYAGPLALGGRYDEVGSAFGRARPATGFSLDLRGVVTALPPATRKPAILAPGVR
                     DAALDNKIEALRQQGEVVIQELPGHE"
     gene            complement(1086..1271)
                     /locus_tag="VN084_03675"
     CDS             complement(1086..1271)
                     /locus_tag="VN084_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HWT28588.1"
                     /translation="MNSSLLTAFALMLILEGVMPLLAPKAWRQTFQRMTALKDGQLRF
                     IGLVSMLGGVLLMLFLR"
     gene            complement(1284..2156)
                     /gene="hflC"
                     /locus_tag="VN084_03680"
     CDS             complement(1284..2156)
                     /gene="hflC"
                     /locus_tag="VN084_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWT28589.1"
                     /translation="MKNLGTILLTIIAALLLLSLSAFTVDQREYALVFRLGEVVSVKK
                     EPGLYFKVPMVENVRYFDKRILTLNWVEPDRFLTSEKKNVLVDSFVKWRIVDPMKYYV
                     SVKGDELQAERRLSQTVNDGLRAEFGKRTIHEVVSGERSKIMEILRQRADRDSRQMGI
                     QVLDVRLKRVDLPQEVSESVYQRMEAERKRVANELRSQGAGAAEKIRADADKQREVII
                     AEAFREAQRIKGEGDAKASEIYAQAYGKNPEFYAFYRSLEAYRNSFKNKSDVFVLEPD
                     SDFFKYLRSSGGKK"
     gene            complement(2171..3349)
                     /gene="hflK"
                     /locus_tag="VN084_03685"
     CDS             complement(2171..3349)
                     /gene="hflK"
                     /locus_tag="VN084_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HWT28590.1"
                     /translation="MANDPGWGNRNNEGPPDLDEVLRQFSRKLSGLFGRQGGGNQPSP
                     DNRAVAILPVIGLIALIWFATGFYIVDQGSRGVVLRFGKHVETTMPGPRWHMPYPIES
                     VTVVNMEQVRTIEVGYRSAEGGAGRSKELRESLMLTDDENIIDLQFAVQYNLKNVEDT
                     LFNNRGAEDSVRGVAETAIREIVGKSKMDFALYEGREEIAVGAKKLMQDILDRYSTGI
                     NIVSVTMQNAQPPEQVQAAFDDAVKAGQDLERQKNEGQAYANDVIPKARGTASRLLEE
                     AAGYKLRVENEAKGNASRFEQVLSQYQRAPEVTRQRLYLDAQEQILSNVSKVVVDQKG
                     GNSLLYLPLDKLMSLTGTNPPTNSSTSPSAVMPNTVLPEQDPSQRTRDAFRSRERESR
                     "
     gene            complement(3389..4516)
                     /gene="hflX"
                     /locus_tag="VN084_03690"
     CDS             complement(3389..4516)
                     /gene="hflX"
                     /locus_tag="VN084_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWT28591.1"
                     /translation="MFDRPSGGSAAILVSVDFGEPDYEDSLLELNQLVTSAGLEVKGV
                     VQGRRPKPDAKYFIGSGKAEQLSSEMKAADAHIAVFNHDLTPSQQRNLERLLEARVVD
                     RTGLILDIFAQRAQSHEGKLQVELAQLEHLSTRLVRGWTHLERQKGGIGVRGGPGETQ
                     LELDRRMLRVRVKQLREKLDKLKQQRGMQRRSRRRSQVMSVSLVGYTNAGKSTLFNRL
                     TQSGVYAADQLFATLDTTSRKLFIPEAGPVVLSDTVGFIKQLPHALVEAFGATLEEAA
                     QADLLLHVVDTASGNRDEQILQVNKVLDEIGASRVPQVLVLNQIDRVGMEPGLERDEY
                     GRICKVRVSAKTGEGMHLLRQVMAEHQQILAEQAAQYSELA"
     gene            complement(4523..4768)
                     /gene="hfq"
                     /locus_tag="VN084_03695"
     CDS             complement(4523..4768)
                     /gene="hfq"
                     /locus_tag="VN084_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWT28592.1"
                     /translation="MNAKGQMLQDPFLNTLRKEHVPVSIYLVNGIKLQGQVDSFDQYV
                     ILLKNTVTQMVYKHAISTIVPARAVSIPPAHSNSSED"
     gene            complement(5023..>5173)
                     /locus_tag="VN084_03700"
     CDS             complement(5023..>5173)
                     /locus_tag="VN084_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWT28593.1"
                     /translation="SHELKFPKVAMPLRVMLTGGAQSPSIDAVMAILGKTETLARIDA
                     YFASL"
BASE COUNT         1082 a         1538 c         1401 g         1152 t
ORIGIN      
        1 ttcgtgccca ggcagttcct ggatcacaac ctcgccctgc tgacgtaacg cctcgatctt
       61 gttgtccagc gccgcatcac ggacccccgg cgcaaggatg gctggctttc tcgttgcggg
      121 cggcagtgca gtgaccacac cacgcaggtc gagactgaac cctgtcgctg gtcgcgcgcg
      181 accaaaagcg gagcctactt catcatatcg cccacccagg gccaatggcc cggcatagcc
      241 ttgagcataa gccgcgaaca cgatgccgct atggtagtga tagccgcgca attcagagag
      301 gtcgaaacaa ggatcgacgc cgaggtcgag tagccgttcc ccgacttgcg ccaagtcatc
      361 cagcgctttg gcgatttcgg cagtgtgtgg caaacgctgc cgtgcgacct cgatgacctc
      421 aagcccaccg ttcaattcgg tcaggctgca aagcgcatct cgcgtgacag gatcaagatc
      481 actggccaag acacgcacag ctgattggtc tttgctttgc aaagcaccat agagcgcttg
      541 ctctagctgg tccgatatcc tgttctgtct gatgaggttg ccgaataacc cgacatgact
      601 gaagtcgatg tgcagggact ggatcccgat cgcctgcagg gctttgacca tgaggcgctg
      661 gatctcgata tcgctctcca cacctgcatg cccgaacagc tcagccccga cctgtaaagg
      721 ctcgcgcgtc tgggcaagcc cgtcaggatt ggtgcgcagc acactacccg catagcaaag
      781 ccggctcacc ccttggtgat tgagcatgtg tgcatcgatg cgcgctgtct gcggcgtgat
      841 atcggcacgc acgcccatga gccgacctgt cagctgatcg accaccttga aggttgccag
      901 atccagatca tgaccgacgc cggtgatgag cgactcgagg tattcgagca tgggcggaat
      961 gacatattga tacccgtgaa ccttgaacag gtcgagcagt cgacgccgaa gctgttcgat
     1021 acgctcggct tcagccggca acacatcttc cagatattcg ggcaataacc agttacgcat
     1081 ggcagttatc tcagaaaaag catgagcaga acaccgccca gcatcgacac caagccgatg
     1141 aatcgcaact ggccgtcctt cagcgccgtc atgcgctgga aggtctggcg ccaggctttg
     1201 ggcgccagca gaggcatgac gccctcaaga atgagcatga gcgcgaaggc ggtgagtaag
     1261 gagctattca atgtcgcgct gcgctatttt ttgccgccgg agctacgtag atacttgaag
     1321 aaatcggaat ccggttcaag tacaaacaca tcagacttgt tcttgaagct gttcctgtac
     1381 gcttcgagac tgcggtagaa cgcatagaat tccggattct tgccgtaggc ctgcgcgtag
     1441 atctcagagg ccttggcatc gccttcgccc ttgatacgct gggcttcacg gaaagcttcc
     1501 gcgatgatga cttcgcgttg cttgtcagca tcggcgcgaa tcttctccgc cgcaccggca
     1561 ccttgtgagc gcaactcgtt ggcgacgcgc ttgcgttcag cttccatacg ctgatagacg
     1621 gattcactga cttcttgtgg cagatcgaca cgcttcaggc gcacatccag cacctggatg
     1681 cccatttggc gcgaatcacg gtctgcacgc tgacgcagga tctccatgat cttgctacgc
     1741 tcgccggaaa ccacttcatg aatcgtgcgc ttgccgaact ctgcacgcag gccatcattc
     1801 accgtttgtg acagacgccg ctccgcctgg agctcgtcgc ccttgaccga cacataatat
     1861 ttcatcggat cgacgatgcg ccacttgacg aaagaatcga ccaacacgtt tttcttctcg
     1921 ctggtgagga accgatcagg ctcgacccaa ttgagcgtga ggatacgctt gtcgaaatat
     1981 cgcacgtttt cgaccatcgg caccttgaaa tacaggcctg gctccttctt caccgagacc
     2041 acctcaccca gcctgaacac cagcgcatat tcgcgttgat ctaccgtaaa ggcagagagg
     2101 ctgagcagca gcaaggccgc gatgatggtc agcagaatcg ttccgaggtt tttcatcaat
     2161 gcatcattca ttatcggctc tcccgctcac ggcttcggaa agcgtcgcgc gtacgttgcg
     2221 agggatcctg ttctggcagg acggtatttg gcatcacggc cgatggcgag gtggaactat
     2281 tggtcggcgg attcgtgccc gtgaggctca tgagcttatc cagtggcaga tagagcaggc
     2341 tattcccgcc cttctggtcg accaccacct tgctgacatt gctcaggatc tgctcttgcg
     2401 catccaggta gaggcgctgg cgggtgactt cgggggcgcg ctgatattgc gacagcacct
     2461 gttcgaaacg actggcgtta cccttggctt cattctcgac gcgcaatttg tagccggcgg
     2521 cttcttccag caagcgcgaa gcagtaccgc gcgccttagg gataacatcg ttagcgtagg
     2581 cttggccttc gttcttctgg cgctcaagat cctgacctgc cttcacggca tcgtcaaaag
     2641 ctgcctgcac ctgctctggc ggctgcgcat tctgcatggt gacgctgaca atgttgatgc
     2701 cagtgctgta acggtcgagg atatcttgca tcagcttctt ggcgcctaca gcgatctctt
     2761 cccgtccttc gtacaaggcg aaatccatct tgctcttacc gacgatctcg cgaatggccg
     2821 tctcggcgac gccacgtacg gaatcctcgg caccacgatt attgaacaat gtgtcttcga
     2881 cgttcttgag gttgtactgg accgcaaact gcaggtcgat gatgttctcg tcgtcagtca
     2941 gcatgagcga ttcacgcagc tccttgctac gccctgcgcc tccctcggcc gagcgatagc
     3001 ccacttcgat ggtgcggacc tgctccatgt tgaccaccgt gacgctctcg atcggatacg
     3061 gcatgtgcca gcgcgggccc ggcatcgtcg tctcgacatg cttgccgaaa cgcaagacca
     3121 cgccacgcga gccttggtcg acgatataga acccggtagc gaaccagatc agggcgatca
     3181 aaccgatcac cggcaggatc gcgacggcgc ggttgtcagg actgggctga ttgcccccgc
     3241 cctgacgccc gaacaggcca ctgagcttgc ggctgaattg gcgcagcacc tcgtccaaat
     3301 cgggcggtcc ttcgttgttg cggttgcccc agccgggatc gtttgccatg catcaactcc
     3361 aaaacaatga ttaatctata ccgcgatgtc aggccagctc ggagtactgt gcggcctgct
     3421 ctgccaggat ctgctgatgt tcggccatca cctgccgcaa aaggtgcatc ccttcaccgg
     3481 ttttggccga aacacgaact ttgcaaattc taccatattc atcgcgctcc agccccggct
     3541 ccatgccgac acgatcgatc tggttaagca ccaggacttg cggcacgcga gaggccccga
     3601 tctcatccag caccttgttc acctgcagga tctgctcatc ccggttaccg ctcgccgtat
     3661 cgacgacgtg cagaagcaga tcggcctggg ccgcttcttc cagggtcgca ccgaaagctt
     3721 cgaccaatgc atgcggcaac tgcttgatga aacccactgt atcggatagc accaccggcc
     3781 cggcttccgg tatgaatagt ttgcgcgagg tggtatcgag cgtcgcaaag agctggtccg
     3841 cagcatagac tccagattgt gtcaggcgat tgaacaaggt ggatttgcct gcgttggtat
     3901 agccgaccag cgacaccgac atgacctgcg aacgcctgcg tgaacggcgt tgcatgccgc
     3961 gctgctgctt gagtttatcg agcttctcgc gcaactgctt gacgcgcaca cgcaacatcc
     4021 ggcgatctag ttcgagctgg gtttcaccag ggccgccacg aacgccgata cccccctttt
     4081 gtcgctcaag gtgggtccag ccacgcacaa ggcgcgtgga aagatgttcg agctgcgcca
     4141 actcgacttg cagcttaccc tcatggcttt gggcccgctg ggcgaagata tcgaggatga
     4201 gtccggtacg gtcgaccaca cgcgcctcga gcaagcgttc tagattacgt tgttgcgagg
     4261 gcgtcaggtc atggttgaac acggcaatgt gggcgtccgc cgctttcatt tcactggata
     4321 gctgttcggc cttgccacta cctatgaaat acttggcatc cggcttgggg cggcggccct
     4381 gaaccacccc tttaacctca agaccggcac tggtaacgag ctggttcaac tccagaaggc
     4441 tatcctcgta atccggttcg ccaaaatcga cgctgaccag gatagcggcg cttccgccgc
     4501 tcggacgatc aaacatcgcg cattaatctt cgctactgtt gctatgggct ggagggatac
     4561 tgacggcgcg ggcaggcacg atggtcgaaa tggcgtgctt gtacaccatt tgggtcaccg
     4621 tgtttttcaa caggatcaca tattgatcga aagagtcgac ctgaccttgc agcttgatcc
     4681 cgttgacgag atagatggat accggcacat gttccttgcg cagggtgttc aaaaatgggt
     4741 cttgtagcat ctgcccttta gcgttcattt attgctcctt ttccttttag ttctggatat
     4801 atagggagtt tttcgttatc cggcaactct acgtcaaatc catcagactc gccagtacta
     4861 gaaaccgcat ttgcctgcct tgctcacatg ggggcggatt cgcccttctc aagtcgcatc
     4921 tgcaagccaa gcatgaatca tgcgcacgaa gtcctgcacc ctgaacacta cgactatgcg
     4981 ccaatgaaat tgttcgtcgt tgcatcgatt tttatgaata actcagaggc ttgcgaaata
     5041 agcatcaata cgggccaggg tctcggtctt gcccaatatc gccatgacag catcgatact
     5101 gggtgattgg gctccacccg taagcatcac gcgcaatggc atggcaacct tgggaaactt
     5161 cagctcatgg ctg
//
LOCUS       DATMTW010000194         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_353316, whole genome shotgun sequence.
ACCESSION   DATMTW010000194 DATMTW010000000
VERSION     DATMTW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353316"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VN084_03705"
     CDS             <1..214
                     /locus_tag="VN084_03705"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWT28594.1"
                     /translation="TRILWLSGCEVGRNRLGNVDSMQRYIYIHGTPDDQTIGEPHSHG
                     CVRMRNDDVIALFDMVPVGTAVEILD"
     gene            392..1300
                     /gene="nikB"
                     /locus_tag="VN084_03710"
     CDS             392..1300
                     /gene="nikB"
                     /locus_tag="VN084_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWT28595.1"
                     /translation="MIRRLMAIVPVVFGVLLLTFLLVHLVPGDPVEVMLGESASGADR
                     AQLRAELGLDQPVVVQFGHYLARLAQGDFGTSIHSGKAISSLLAERYPATLKLALLAL
                     CIALCIGLPLGIVAALKADRWPDHVATTLSLTLSAMPHFWLGPLLMLVFALWLGWLPV
                     SGMENGASILLPALTLGFGLAAILTRMTRASLLEVLNEDFVRTARAKGLDESTVILRH
                     ALRAALLPVMTVVGLQMGSLLAGTVITETVFAWDGIGRLLVESIEKRDYPVTQACVLV
                     IALSYVLVNLGTDLLYARLDPRVRLD"
     gene            1300..2088
                     /locus_tag="VN084_03715"
     CDS             1300..2088
                     /locus_tag="VN084_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWT28596.1"
                     /translation="MRRLPYLILLFWIAAVCIAWQLDLHPNLISLDRILAAPDHAAWL
                     GNDDLGRDILARLLSGAEVSFLVATIVTGITLSFGVMVGLVSGFYGGWIDRTLMQITD
                     VFLAFPGILLAIAFAAVLGPGLNNLILALCLTSWVGYARLTRAQALSLRQSPHVLAAE
                     SLGAGVPRMMLRHMLPLLAAPLVVEATYSIAGLVIAEASLSFLGLGIQAPEASWGAML
                     RDGVRFLLVAPHYVLAVGVSLMSLVLAINLLGDRLRDHWDVKRV"
     gene            2177..2905
                     /locus_tag="VN084_03720"
     CDS             2177..2905
                     /locus_tag="VN084_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HWT28597.1"
                     /translation="MTKPLTKPLTQPRQYRYYDLMMAAFVTVLVCSNLIGPAKVAQIE
                     LPWVDTLTFGAGIMFFPISFIFGDILTEVYGYARSRRVIWAGFMALAFASFMAWVIVA
                     LPPAPFWHNQHEYEVAFGSAWRVSLACLVAFAAGEFVNSYIMAKMKIWTQGRHLWGRT
                     IGSTIFGEGVDTLLFFPLAFYGTGVIPDDQILYIVWIQFVAKVGVEVAFTPITYKIVA
                     WLKKVENEDYYDRETDFNPFTLKS"
     gene            2909..>3910
                     /gene="nagZ"
                     /locus_tag="VN084_03725"
     CDS             2909..>3910
                     /gene="nagZ"
                     /locus_tag="VN084_03725"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWT28598.1"
                     /translation="MSLGPVMLDVVGTELTDDDIRRLQHPLVGGVILFARNFVSCTQL
                     KALTAAIHAIRQPPLLIAVDHEGGRVQRFREGFTRIPPMREFGKIWDKHPKRARQLAE
                     EAGWILAAELRAHGIDFSFTPVLDMDYGESLVIGDRAFHRDAQAINELAFSLMQGLKK
                     GGMPAVGKHFPGHGYVRADSHVAMPVDERSFDEIAQVDMQPFRQLIDDGLPAIMPAHV
                     IYAKVDDKPAGFSEKWLQKVLRQRLEFNGVIFSDDLSMEAASAGGNVTERALAALRAG
                     CDMVLLCNQPAMADELLANLKWDISGQSLARLARMHGRHHPADMVALREDAAFMQAIH
                     "
BASE COUNT          631 a         1227 c         1233 g          819 t
ORIGIN      
        1 gacgcgcatc ctctggctct ccggctgcga ggtcggccgc aaccggctcg gcaacgtcga
       61 tagcatgcag cgctacatct atatccacgg cacaccggac gaccagacca tcggcgagcc
      121 gcattcccat ggctgtgtgc gcatgcgtaa tgacgatgtg atcgccttgt tcgacatggt
      181 gccggtaggg actgcggttg agatcctgga ctgaccgccg atactcgccg atgcacgcgg
      241 atcacaacgg cgccagccat ggcttgcagc aggtgcatgc cgacgtctgg cccggacggc
      301 tgattcccat cgatcgacaa atgcttgccc atcagcctgc ggctaggtcg atcggcgttg
      361 atgggccttc atctgcggtg acatcccaat gctgatcagg cgcttgatgg cgatcgtgcc
      421 agtggtgttc ggcgtgttgc tgctgacctt cctgctggtg catctggtgc cgggcgaccc
      481 ggtcgaggtg atgctgggcg aatcggcgag cggcgcggac cgcgcgcagc tgcgtgccga
      541 actgggactg gaccaacctg tcgtcgtgca gttcggccat tatctggcca gactcgcaca
      601 gggcgacttc ggcacatcca tccatagcgg taaggcgatc tcgtcgttgt tggccgagcg
      661 ctacccggcg acgctgaagc tggccctgct ggctttgtgc atcgccctct gcatcggcct
      721 gccgctgggc atcgtcgccg cactcaaggc cgatcgctgg cctgaccatg tggcgaccac
      781 gctcagcctc acgctatcgg ccatgccgca tttctggctc gggcctctgc tcatgctggt
      841 gttcgccctg tggctgggct ggctaccggt cagcggcatg gagaacggcg cgtcgatcct
      901 gctgcccgcg ctgaccctgg ggtttggcct tgcggccatt ctcacgcgca tgacgcgcgc
      961 cagcctgctg gaagtgctca acgaggattt cgtgcgtacc gcgcgggcca aggggttgga
     1021 cgagtcgacc gtgatcctcc gccatgcctt gcgtgcggcg ctgctacccg tgatgacggt
     1081 ggtcggcttg cagatgggca gcctgctggc cggcaccgtc atcaccgaga ccgtgttcgc
     1141 ctgggacggc atcgggcgcc tgctggtcga atccatcgag aagcgtgatt acccggtgac
     1201 ccaggcctgc gtgctggtga tcgcgctcag ttatgtgctg gtcaacctgg gcacggacct
     1261 cctgtatgcg cgtctggatc cacgagtgag actcgactga tgcggcgtct gccttacctc
     1321 atcctgttgt tctggatcgc ggccgtatgc atcgcctggc agcttgacct gcatcccaac
     1381 cttatcagcc tggatcgcat cctggcagca ccggaccatg ccgcatggct gggtaacgac
     1441 gatcttggcc gcgatatcct cgcgcgactg ttgagtgggg ccgaagtctc gttcctggtg
     1501 gcgaccatcg tcaccggcat cacgctcagc ttcggcgtga tggtgggctt ggtatccggc
     1561 ttctacggcg gctggatcga ccggacactg atgcagatca ccgatgtgtt cctggctttc
     1621 cccggcatcc tgctggcgat cgcgttcgcc gccgtgctcg gccccggact caataacctg
     1681 atcctcgcct tgtgcctgac ttcgtgggtg ggctatgcgc ggctgacgcg tgcgcaggcc
     1741 ttgtccctgc gacaaagccc gcatgtgctg gcggcggagt cgctcggcgc cggtgtgccg
     1801 cgcatgatgc tgcgccacat gctgccgttg ctggcggcgc ctctggtggt cgaggcgact
     1861 tacagtatcg ccggcctggt catcgccgaa gccagcctgt ccttcctcgg tttggggatc
     1921 caggcgcccg aggcttcctg gggtgccatg ctgcgcgacg gcgtacgctt tttgctggtc
     1981 gcgccgcatt acgtgctggc agtcggtgtc agcctgatgt cgctcgttct ggcgatcaac
     2041 ctgctgggtg accgtttgcg cgaccattgg gacgtgaagc gcgtatgaaa cctgctgcgc
     2101 gacgtatctg cttcgttatg cgatgctcgc aaggtttctc agctgatatg atgcagaaaa
     2161 ttcacactgc ccagctatga ctaagccgct gaccaaacca ctgactcaac cgcgccaata
     2221 tcgttattac gacctcatga tggcggcctt cgtcaccgtg ctggtctgtt ccaacctcat
     2281 cggcccggcc aaggtggcgc agatcgagct gccgtgggtc gatacgctga ccttcggcgc
     2341 cggcatcatg ttctttccga tctcgttcat cttcggcgac atcctgaccg aggtctacgg
     2401 ctatgcccgc tcgcggcgcg tgatctgggc cggtttcatg gcactggcgt ttgcctcgtt
     2461 catggcctgg gtcatcgtgg cgctgccgcc agcgccgttc tggcataacc agcacgaata
     2521 cgaagtggcg ttcggctcgg catggcgcgt gtcgctggcc tgcctggtgg cgttcgccgc
     2581 cggcgagttc gtcaactcct acatcatggc gaagatgaag atctggacac agggccggca
     2641 cttgtgggga cgcaccatcg gttccaccat cttcggcgaa ggcgtcgata cgctgctgtt
     2701 cttcccgctg gcgttctacg gcaccggcgt catcccggac gaccagatcc tctatatcgt
     2761 ctggatccaa ttcgtggcca aggtcggggt ggaagtggca ttcacgccga tcacctataa
     2821 aatcgtggcc tggctgaaaa aggtcgaaaa tgaagactat tacgaccgcg aaaccgattt
     2881 caatcccttt accttgaaga gttaagttat gtccctaggc ccagtgatgc tggatgtggt
     2941 cggcaccgag ctgaccgatg acgatatccg ccgcctgcag catccgctgg tcggtggcgt
     3001 tatcctgttc gcccgcaact tcgtctcgtg cacgcaacta aaagcgctga ccgcggcgat
     3061 ccacgccatc cgccagcccc ccttgctgat cgcggtggac cacgaaggcg gccgtgtgca
     3121 gcgttttcgc gaaggtttca cgcgcatccc gcccatgcgc gaattcggca agatctggga
     3181 taagcatccc aagcgggcgc gccaactggc ggaagaggct ggctggatac tggccgccga
     3241 actgcgcgcg cacggcatcg atttcagctt cacgccggtg ctggacatgg attacggcga
     3301 gagcctggtg atcggcgacc gtgccttcca ccgcgacgcg caagccatca acgaacttgc
     3361 cttttcgctc atgcagggcc tgaagaaggg cggcatgccg gctgtcggca agcatttccc
     3421 tggccacggc tacgtccgtg ccgattcgca tgtggcgatg ccggtggatg aacgcagttt
     3481 cgacgagatc gctcaggtcg acatgcagcc gttccgccag ctgatcgacg acggcttgcc
     3541 ggccatcatg ccggcgcatg tgatctatgc caaagtggat gacaagccgg ccgggttctc
     3601 cgagaagtgg ctgcagaagg tattgcgcca gcgcctggaa ttcaacgggg tgatcttcag
     3661 cgatgacctg tcgatggaag cggcgtcggc cggcggcaac gtcaccgagc gcgcgctggc
     3721 ggcgctgcgt gcgggctgcg acatggtcct gctgtgcaat cagccggcga tggcggacga
     3781 attgctggcc aatctaaagt gggacatcag cggccagagc ctcgcccgcc tggcacgcat
     3841 gcatggccgt catcatccgg ccgacatggt cgcgctgcgc gaggatgcgg cgttcatgca
     3901 ggccatccac
//
LOCUS       DATMTW010000195         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_51411, whole genome shotgun sequence.
ACCESSION   DATMTW010000195 DATMTW010000000
VERSION     DATMTW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51411"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..178
                     /locus_tag="VN084_03730"
     tRNA            89..178
                     /locus_tag="VN084_03730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Ser,seq:cga)
     gene            complement(230..1471)
                     /locus_tag="VN084_03735"
     CDS             complement(230..1471)
                     /locus_tag="VN084_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28599.1"
                     /translation="MDHAQYISHNLAALKQILLASTSEEDGKNLIQDILKSLRTEIGM
                     DVAFIAEFSEGKRIFRYIDASEESPPVKPGDGDPIEHTYCQRVINGQLPDIIFDISSL
                     PPHEQLAVAEDYKIRSYLCVPITFADGRIYGSLGCIGHQPNWQINQQDIQLMSAMAEV
                     YGQWFNAQERIHDHQLDMEEKVRAALSGQLLEVLYQPIFDITQDKVVGFESLARIHSK
                     DGLNPLSLFREAEALGHKVELELKAIRLGLQGLDYYRETQFVSVNASMDTALSPQLPD
                     VLEHADMKRVVLEINDPMDLPIFSRVTKSLRALLNRGLRIALDDRALGTEGLKQLDSL
                     GPDIIKLKVRNVLHMKGDRLLKLQASPFSGYSEENTCKLIVEGIETEQQLKTILELGI
                     THAQGYWLGEPIGLLEAAERY"
     gene            complement(1514..>4030)
                     /locus_tag="VN084_03740"
     CDS             complement(1514..>4030)
                     /locus_tag="VN084_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28600.1"
                     /translation="EHYLVAVEDITERRTAENALRQQEEFNRVLLNNQADGVVACDAD
                     MRLVVFNNIARQWHGLDPREIPPEQWSRYFGLYDASGEHELQAPEIPLVRAFNGEQLQ
                     HAGMVIKAQGQAPRFVSCSATAFFDDQGKKLGAVAIMRDITEIQAQDRALRESETLFQ
                     ALARVAPAGIFRTNAMGANTYINRRGLEILGLTTEADALGSGWARHLHPDDTEPLLQA
                     WKIAFSQRATFSAEYRFLHPDGKLIWVKGEAQVESDDKGTFLGYVGTITDITAIKQSE
                     ENLRMAAAVFDNTREGIMVTDQASRILSVNRAFSEITQYEAAEVIGKTPKLLQSGKHE
                     AHFFEAMWQALNTEGYWQSEIWNRRKNGELYPQLLSISVIRDPLGAVQNYVGIFADIT
                     NLKASEARLDFLAHHDPLTNLPNRLRLISNLEHAIQVAQREQKQLALLMLDLDRFKDV
                     NDTFGHLAGDELLQHAAKRLTRRLRGADTVARLGGDEFTILLEDINLVSDSAKVADSI
                     IKALEAPCKLANNVEVHIGVSIGISIYPEHGSTALELLQHADAALYQAKAAGKGCYRY
                     FSETLTKAARDRFSIESRLRAAIEKDQLALYYQPKIDIASGKIVGAEALIRWIDPVEG
                     VIMPGSFISVAEETGMISRIGEWVIRTACAQGRHWLDQGLPPMAFSVNISANQLHHGD
                     LYRTIRDILAETGFPATSLELELTESILMQREKEVLETLSQIRSAGVVIGIDDFGTGY
                     SSLAYLKSFPLDVLKIDRGFVADIEYDADDRAITATIIAMAHTLGMQVVAEGVENEAQ
                     LTFLKTLQCDIYQGYLISKPLPADAFETFMREYGNRSGAV"
BASE COUNT          722 a         1163 c         1253 g          892 t
ORIGIN      
        1 gtccagcgag aaaatctcgg taaatatgac agccgatggt agaatagcgc ctcgcgcggc
       61 tggcggtcca aaccggcctt gcgatgcagg agaggtggca gagtggttga atttacctga
      121 ctcgaaatca ggcatacgtg caagcgtatc gagggttcga atccctccct ctccgccacc
      181 ggtatcaagc gatcttcccg tcgtatctcg cgcttcagtc tctttcccct cagtatctct
      241 cggcagcttc cagtaatccg atcggctcgc ccagccagta gccttgcgca tgtgtgatgc
      301 ccagctcgag aatggtcttc agctgctgct cggtctcgat gccttcgacg atgagcttgc
      361 aggtgttctc ctctgaatag ccgctgaacg gtgaggcctg gagcttgagc agacggtcgc
      421 ccttcatgtg caacacattc cggaccttga gcttgatgat atccggcccc aggctgtcga
      481 gctgcttcag accttccgtc cccagtgcac ggtcgtctag cgcgatgcgc aggccgcggt
      541 tgagcagggc gcgtagcgat ttggtcaccc ggctgaagat cggcaggtcc atcggatcgt
      601 tgatctcgag caccacccgc ttcatgtcgg catgctcgag cacatccggt aattgtggtg
      661 aaagcgccgt atccatcgat gcgttgaccg agacgaactg cgtctcgcgg taataatcca
      721 ggccttgcag gcccagacgg atggctttca gctcaagctc gaccttgtgc ccgagcgctt
      781 cggcttcacg gaacagggag agcggattga ggccatcctt gctgtggatg cgcgccagtg
      841 attcgaagcc caccaccttg tcctgcgtga tgtcgaagat gggctggtag agcacctcga
      901 gcaactggcc gctcaatgcc gcccgcactt tctcttccat atcgagctgg tggtcgtgga
      961 tcctttcctg ggcgttgaac cactggccat agacctcggc catggccgac atcagctgga
     1021 tatcctgctg gttgatctgc cagttgggtt gatggccgat gcagcccaac gaaccataga
     1081 tgcggccgtc tgcgaacgtg atgggcacgc agagataaga gcggatcttg taatcctcgg
     1141 cgacggccag ttgttcgtgc ggcggcaggc tgctgatatc gaagatgatg tccggcaact
     1201 ggccgttgat gacgcgttgg caataggtgt gctcgatcgg gtcgccatcg ccgggtttta
     1261 ccggcggcga ctcctcggag gcgtcgatat aacgaaagat acgcttgccc tcgctgaact
     1321 cggcgatgaa cgcgacatcc atgccgatct cggtacgtag cgacttgagg atgtcctgga
     1381 tcaggttctt gccgtcttct tcagaagtcg acgccagtaa tatctgcttc agtgccgcca
     1441 ggttatggga gatgtattgc gcatgatcca tgctcgattc cgacctcttt tttatgatta
     1501 tcgtggtttg agactacacc gcgccagatc ggttgccata ttcgcgcata aaggtttcga
     1561 aagcgtctgc cggtaacggc ttactgatga gatagccctg gtagatatcg cactgcaggg
     1621 tcttgaggaa cgtcagttgc gcctcgttct ccacgccttc ggccactacc tgcatgccca
     1681 gcgtgtgagc catggcgatg atggtcgccg tgatggcacg gtcgtcggcg tcatactcga
     1741 tatcggcgac gaaaccacga tcgatcttca gcacatccag tgggaagctc ttcagatagg
     1801 cgagggacga gtagcccgtg ccgaaatcgt cgatgccgat cacgacgcca gcgctgcgga
     1861 tctggctcag ggtttccagc acctctttct cgcgttgcat gaggatgctt tctgtcagtt
     1921 ccagctccag cgaagtagcc gggaaaccgg tttcggcgag aatgtcccgg atggtgcgat
     1981 agaggtcgcc gtgatgcagc tggttggcgg agatgttcac cgagaacgcc atcggcggca
     2041 ggccttgatc cagccagtgt ctgccctgtg cgcatgccgt acggatcacc cattcgccga
     2101 tgcggctgat catgccggtc tcttcggcga cgctgatgaa actgccgggc atgatcacgc
     2161 cctcgaccgg gtcgatccag cggatcagcg cttcggcgcc gacgatcttg ccgctggcga
     2221 tatcgatctt gggctggtaa tagagcgcga gctggtcctt ctcgatggcc gcccgtaacc
     2281 ggctctcgat gctgaagcgg tcgcgagcag ccttggtcag cgtttctgag aaatagcggt
     2341 aacagccctt gccggccgcc ttcgcctggt acagagcggc atcggcatgc tgcagcagtt
     2401 ccaaagccgt actgccatgt tccggataga tgctgatgcc gatactgacg ccgatgtgga
     2461 cctcgacatt attggcgagc ttgcagggtg cctccagcgc cttgatgatg ctgtcggcga
     2521 cttttgcgct atccgagacc aggttgatat cttcgagcag gatggtgaat tcgtcgccgc
     2581 cgaggcgggc gacggtatcg gcaccgcgca gacgacgcgt gaggcgcttg gcggcgtgct
     2641 gcaagagctc gtcgccagcg agatgcccga acgtgtcgtt gacgtccttg aaccggtcga
     2701 gatccagcat cagcagggcg agttgctttt gctcgcgctg cgcgacctgg atggcgtgct
     2761 cgagattgga gatcagccgc agccgattcg gcaggttggt cagcggatca tggtgggcaa
     2821 ggaaatccag ccgcgcctcg gatgccttga gattggtgat gtcggcgaag atgccgacat
     2881 agttctgtac tgcgccgagc gggtcgcgga tcaccgagat gctgagcagc tgcggataga
     2941 gctcgccgtt cttgcggcgg ttccagatct cgctctgcca gtagccctcg gtattcagcg
     3001 cctgccacat ggcttcgaag aagtgggcct cgtgtttacc ggattgcagc agctttgggg
     3061 tcttgccgat gacttcggcc gcctcgtact gggtgatctc gctgaaagcg cggttgaccg
     3121 agaggatgcg tgacgcctgg tcggtgacca tgatgccctc gcgcgtgttg tcgaagacgg
     3181 cggcggccat gcgcaggttc tcctcacttt gcttgatggc cgtgatatcg gtgatggtgc
     3241 cgacataacc cagaaaggtg cctttgtcgt cgctctcgac ctgtgcctcg cctttgaccc
     3301 agatcagctt gccgtcagga tgcaggaaac ggtattcggc gctgaaggtc gcacgctggc
     3361 tgaaagctat cttccaggcc tgcaacaacg gctccgtgtc gtccgggtgc aaatgccgtg
     3421 cccagccaga acctagggca tcggcttctg tggtcagccc gaggatctcc aagccgcgcc
     3481 ggttgatata ggtattggcg cccatggcgt tggtgcggaa gatgccggcg ggcgcgaccc
     3541 gcgccagtgc ctggaacagg gtctcgctct cgcgcagggc acggtcttgc gcttgtatct
     3601 cggtgatgtc gcgcatgatg gcgaccgcgc cgagcttctt gccctggtcg tcgaagaaag
     3661 ccgtcgcgct gcaggagacg aagcgcggtg cctggccctg tgccttgatc accatgccgg
     3721 catgctgcaa ctgctcgccg ttgaaggcac gtaccagcgg gatctcgggc gcctgcagtt
     3781 cgtgctcgcc actggcatcg taaagaccga agtaacgact ccattgctcg ggcgggatct
     3841 cgcgcggatc gaggccatgc cattggcgcg cgatgttgtt gaagaccacc aggcgcatgt
     3901 cagcgtcgca ggccaccacg ccgtctgcct ggttgttgag cagtacgcgg ttgaattcct
     3961 cctgctggcg cagtgcattc tctgccgtgc ggcgttcggt gatgtcctcg acggcgacca
     4021 ggtaatgttc
//
LOCUS       DATMTW010000196         5348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_419820, whole genome shotgun sequence.
ACCESSION   DATMTW010000196 DATMTW010000000
VERSION     DATMTW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419820"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN084_03745"
     CDS             <1..237
                     /locus_tag="VN084_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HWT28601.1"
                     /translation="FGQTFKFGGLKSILLEDEVLGGAIQLSCSVPCAFSSQPHFSVSQ
                     SEAGFEKIMQAVTLELIWSGDMLSDRIEIKIEIM"
     gene            275..1204
                     /locus_tag="VN084_03750"
     CDS             275..1204
                     /locus_tag="VN084_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWT28602.1"
                     /translation="MKTLLGIDVGGTNLRIGVVKGNHVVHEHRAQVDFSKLCKTHAPD
                     MAWQAIISSMHAGVAEVLARFPDVSAVGIGFPGFIDPVAGVLTQSPNLPGLRDVDLAG
                     DLTRAIGLPVIVENDAAAAAFGEYRLHPVDSDHLIYVGLGTGIGGGLIYADRIFEGQH
                     GMSMEIGHLTIEPDGRSCGCGNRGCMEQYASATGVATTYRLATDSEKTAQEIAALAKS
                     GDRHARYAFELAGTCLGQGLAHIIKVVDVGHVLIGGGMREAWPLMQATFERRFQRDLI
                     PALRSRVTVTLSASGDQAGMIGAALLAGVRSGI"
     gene            complement(1150..3753)
                     /locus_tag="VN084_03755"
     CDS             complement(1150..3753)
                     /locus_tag="VN084_03755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT28603.1"
                     /translation="MRIFFRDYFKPLFFALLIAGIHFGLWWAVNRALPLIDAPRIVHG
                     FAYSGYQLGQSPLDKKYPSTAELLDDLKLLKPYTDRIRTYGSLENPEVIPLASNLGMQ
                     IMAGAWLSSDNIDNEREVSALIAKIRSYPRIHRAIVGNEVLLRTDLTPAQLIRYLDHV
                     REEVSIPVSTAEPWHVWLKNPELVKHVDFIAVHLLPYHEGIPIDKALDYSLQRYQELM
                     DAYPRKKIVITEIGWPSKGPAIGAAVASQVNQARFVREFLARTAHQNYDYYLMEAIDQ
                     PWKVNVEGWAGAYWGMFDAERHLKYDLHGAIPKDTRWIDKAGWASIIAFFPILLAAIR
                     FRHWGTGAQLSLAVLLQACITTLVVAWNLPGDYYYGLRDFVVLIGLIIGMGLTSAVLM
                     IYGVEFSEVMFKGLWYRAFRRATPLPEEQELFVSIHLACYNEPPAMVIATIESLHQLQ
                     YRHFEVIVIDNNTKDEALWKPVEAYMAQLPDNFRFFHLPSWPGFKAGALNFALAQTDP
                     GAEVIGVVDADYAVTQDWLGALVPHFKDTQVAVVQAPQAHRDWENNFFRRMCNWEFEG
                     FFRIGMHHRHERNALIQHGTMTLVRRQSLDEIGGWSEWCICEDTELGLRLLEGGYELR
                     YIDETFGRGLTPTNFKALKSQRFRWAFGAMQILKHHLPRLLGRSTLDFGQRYHFLTGW
                     FGWLGDALQLVFTLGSIGWTLAMLAFPKSFSLPVSIMLTPIICFLVIKAALGPVLYRK
                     TMHCGWSDIFGASLASLGLSHAIARGVMQGIVQKEGVFKPTAKGKSANGGLRQLLEPI
                     REEFLLLLGLIICSLAMLFSRGMNNIDAQLWVAMLSLQSLPYLSAVACQLIAQMPDRT
                     PASKAAPIMPA"
     gene            complement(3762..>5348)
                     /locus_tag="VN084_03760"
     CDS             complement(3762..>5348)
                     /locus_tag="VN084_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta (1-6) glucan synthase"
                     /protein_id="HWT28604.1"
                     /translation="FRAAMPIKDPADQASPRFHSFHILFNLAILIALGFWLWQQNTPA
                     ALVAPPLPPDGKLQCVSYAPYYGKDQTPFKADTVIAPAQIDHDLAILAERFDCVRIYS
                     VDQGLSHVPEAAEKLGMQVLLGAWIGSVSSKNDRELSLAIELANRYPKTVKGLIVGNE
                     VLLRRELTPEALRVYLERAQQGTEVPVTYADVWEFWIKNKSLESAVDYVTVHILPYWE
                     DHPVSIEQGVQHTHDVMFRLASIFSKPLFIGETGWPSVGRQRQGSTPSLVNEARYIRE
                     FLQLAAAQGWHYNLIEAIDQPWKRDLEGAVGGYWGLYGTDLQPKFELSGTVRERHDDW
                     GVLAWPLVGALLWLAYGWMLGLRRHALIATATLGAVTAGFAMLQLEYLVVASRDLVEW
                     LSLGSIAVSGWFIGLLLPRLILARTPLRVLSYQLTLIFLLNAVLVSNYLQLSDGRYRD
                     FPLVLYLLPALQLCFFTWCAGLMPRPASRYTFVIALIGLASAIACTVVEIGSVVSFCW
                     LAIVILFVLTALPVRAHPKA"
BASE COUNT         1112 a         1538 c         1699 g          999 t
ORIGIN      
        1 tttggccaga ccttcaagtt cggcgggctg aaaagcatct tgctggaaga cgaggtgctg
       61 ggcggagcga tccagctctc gtgttccgtg ccttgtgcgt tcagcagcca gccccatttc
      121 tcggtatccc agtcggaagc cggtttcgag aagatcatgc aggcggtcac gctagagctg
      181 atctggtcgg gcgacatgct gtcagatcgc atagagatca agatagaaat aatgtaaaca
      241 aaagcttacg aattaaatat aaagcgaatt attgatgaaa acactgctcg gtatcgatgt
      301 tggaggcacc aatctgcgta tcggcgtggt caagggtaac catgtcgtgc acgagcatcg
      361 ggcccaggtt gatttcagca agctgtgcaa gacccatgcg ccggatatgg cctggcaggc
      421 gatcatatcc agcatgcacg ctggcgtagc agaagtgctg gcgcgcttcc ctgacgtctc
      481 ggcggtcggc atcgggttcc cggggttcat cgacccggtc gccggtgtgc tgacgcaatc
      541 gccgaatctg ccaggcttgc gcgacgtcga tctggccggc gatctcacgc gcgccatcgg
      601 tttgccggtc atcgtcgaga acgatgcggc agcagcggcc tttggcgaat accgcctgca
      661 tccggtcgac agcgatcacc tgatctacgt cgggctgggc acgggcatcg gcggcgggct
      721 gatctatgct gaccgcatct ttgagggtca gcacggcatg tcaatggaga tcgggcatct
      781 gacgatcgag ccggatggtc ggtcatgcgg ttgcggtaac cgcggctgca tggagcaata
      841 tgcctcagcg actggcgtgg cgacgacata ccgactggcg accgatagcg agaaaaccgc
      901 acaggagatc gccgcgctag ccaagagcgg tgatcggcat gcccgttatg cctttgaatt
      961 ggcgggcacc tgtctcggcc aggggctggc tcatatcatc aaggtcgtcg acgtcggcca
     1021 tgtgttgatc ggtggcggca tgcgcgaagc atggccgttg atgcaggcaa ccttcgaacg
     1081 ccgtttccag cgcgatctga tcccggcgct gcgcagccgg gtcacggtga ccctatccgc
     1141 aagcggcgat caggcaggca tgatcggcgc ggccttgctg gccggcgtgc gatccggcat
     1201 ctgagcgatc agctggcagg ccacggcgct gaggtaaggc agggattgca ggctcagcat
     1261 ggccacccac aattgcgcgt cgatgttgtt catgccgcgc gagaacaaca tggccaggct
     1321 gcagatgatc aagccgagca gcagcaggaa ctcctcacgg atcggctcca gcagctgcct
     1381 taatcctcca ttcgcgctct tgcctttggc tgtcggcttg aacacgcctt ctttctgcac
     1441 gatcccctgc atcacgccgc gcgcgatggc gtgcgacagg cccaggctgg cgagcgaagc
     1501 gccgaagata tcggaccagc cacaatgcat ggtcttgcgg tagagcaccg gccccagcgc
     1561 agccttgatc accagaaaac agatgatagg cgtgagcatg atggagacag gcaggctgaa
     1621 cgacttggga aaagccagca tggccagcgt ccagccgatc gaacccagcg tgaagacgag
     1681 ttgcagggca tcgcccagcc agccgaacca gccggtcagg aagtggtaac gctggccgaa
     1741 atcgagggtc gacctgccga gcagccgggg caggtgatgc ttcaatatct gcatggcgcc
     1801 gaaggcccag cggaaacgct gtgatttcaa tgccttgaag ttggtcggtg tcaggccccg
     1861 accaaaggtc tcgtcgatat agcgtagttc atagccgcct tccagcaggc gcaagccgag
     1921 ctcggtgtcc tcgcagatgc accactccga ccagccgccg atctcgtcca gcgattgccg
     1981 gcgtaccagc gtcatcgtgc catgctggat cagggcgttg cgctcatgcc gatggtgcat
     2041 gccgatgcgg aagaagcctt cgaactccca gttgcacatg cggcggaaga agttgttctc
     2101 ccagtctcgg tgagcctgcg gcgcttgcac cacggcgacc tgagtatcct tgaaatgcgg
     2161 caccaatgcg cccagccagt cctgggtcac cgcataatcg gcatcgacca cgccgatgac
     2221 ttcggcgccc ggatcggtct gcgccagcgc aaaattcaat gcgccagcct tgaagccggg
     2281 ccaggacggc aggtggaaga agcggaaatt gtcgggcagc tgcgccatat aagcctcaac
     2341 aggcttccag agcgcctcat ccttggtgtt attgtcgatg acgatgactt cgaaatggcg
     2401 atactgaagc tggtgcaggc tttcgatggt ggcgatcacc atcgccggcg gctcgttata
     2461 gcaggcgagg tggatggaga cgaacagctc ttgttcctcc ggcaagggcg tggcacggcg
     2521 gaatgcgcgg taccacagcc ccttgaacat gacttcgctg aattcgacgc catagatcat
     2581 cagcactgcc gaagtcaggc ccatgccgat gatgagaccg atcagcacga cgaagtcgcg
     2641 cagcccatag taatagtcgc cgggcaggtt ccatgcgacc accagcgtgg tgatgcaggc
     2701 ctggagcagg actgccagcg agagctgcgc cccggtgccc cagtgacgga accggatcgc
     2761 cgccagcagg atggggaaga aagcgatgat cgaggcccag ccagccttgt cgatccagcg
     2821 ggtatccttg ggaatggcgc catgcaagtc atatttgaga tggcgctcgg catcgaacat
     2881 accccagtac gcaccggccc agccttcgac gttgaccttc cacggctggt cgatggcttc
     2941 catcaggtag tagtcgtaat tctggtgggc agtgcgcgcg aggaattcac gcacgaaccg
     3001 cgcctgattg acctgtgacg ccacggcagc gccgatagcc gggcctttgc tcggccagcc
     3061 gatctcggtg atgacgatct tcttgcgcgg ataggcatcc atcagttctt ggtaacgctg
     3121 cagcgaatag tcgagcgcct tgtcgatggg aatgccttcg tgatacggca acagatgcac
     3181 ggcgatgaaa tcgacatgct tcaccagttc aggattcttc agccagacat gccagggctc
     3241 cgccgtcgat accggaatgc tgacttcctc ccgcacatgg tcgaggtagc ggatcaattg
     3301 cgccggcgtg agatcggtgc gcagcaggac ttcgttgccg acgatggcgc gatggatgcg
     3361 cggataactg cggatcttgg cgatcagcgc gctgacctcg cgttcgttgt cgatattgtc
     3421 gctgctgagc caggctccgg ccatgatctg catgccgagg ttggaagcca gcgggatgac
     3481 ttccgggttc tccagcgaac cataggtgcg gatacggtct gtatagggct tgagcagctt
     3541 gaggtcatcc agtagctcgg ctgtgctcgg gtatttcttg tccagagggc tctggccgag
     3601 ctggtagccg ctgtaggcga aaccatgcac gatgcgtggc gcgtcgatca gcggcagcgc
     3661 gcggttgact gcccaccata gcccgaaatg gatccctgcg atgagcagcg cgaagaagag
     3721 tggcttgaaa taatctcgga agaagatacg catgcgtgga ttcaggcttt cgggtgagcc
     3781 ctgacaggca gcgccgtcag gacgaacagg atgacgatgg ccagccagca gaaggagacg
     3841 acgctaccga tctcgaccac tgtgcaggcg atggcactgg cgagaccgat gagcgcgatc
     3901 acgaaggtat agcgtgatgc gggtctcggc atgagtccgg cgcaccaggt gaaaaagcac
     3961 aactgcaggg ccggcagcag atacagcacg agcggaaaat cgcgatagcg accgtcgctc
     4021 agttgcagat agttgctgac caggactgcg ttgagcagaa agatcagggt caactgatac
     4081 gacaacaccc gtaacggggt tcgcgccagg atcagtcgcg gcagcagcag gccgatgaac
     4141 cagccggaaa cggcgatgct tccgagtgaa agccattcca cgagatcacg gctggcgacg
     4201 acgaggtatt ccagctgcag catggcgaac cccgccgtga cggcgcccag cgtcgcggta
     4261 gcaattagcg catggcgacg tagcccgagc atccagccat aggctagcca gagcagggcg
     4321 ccgacaagtg gccatgccag caccccccag tcgtcgtgac gctcgcgtac tgtaccgctg
     4381 agctcgaact tcggctgcag atcggttcca tacagccccc agtaaccacc gaccgcgccc
     4441 tccagatcgc gcttccacgg ttggtcgatg gcctcgatca ggttgtagtg ccaaccctga
     4501 gccgcggcta gctggaggaa ctcgcggata tagcgggctt cgttcaccag gctcggcgtc
     4561 gaaccctggc gctgacggcc gacgctgggc cagccggtct cgccgatgaa cagcggtttg
     4621 ctgaagatgg aagctaggcg gaacatgacg tcatgggtat gttgcacgcc ttgctcgatc
     4681 gagactggat ggtcttccca gtaaggcagg atatgcacgg tcacataatc gaccgcgctt
     4741 tccagcgact tgttcttgat ccagaactcc cagacatcgg cataggtgac cggcacttcc
     4801 gtgccttgct gggcgcgctc caggtagacg cgcaacgcct caggcgtgag ctcacggcgc
     4861 aacaggactt cgttgccgac gatcaggccc ttgactgttt ttggataacg attggcgagt
     4921 tcgatcgcca gcgacagttc gcggtcgttc ttgctgctga cgctaccgat ccaggcgccc
     4981 agcaacactt gcatgccgag cttctcggcg gcttccggta catggctcag gccctggtcg
     5041 acggaataga tacgcacgca atcgaagcgc tcggcaagaa tggcgaggtc gtgatcgatc
     5101 tgcgcggggg cgatgacggt atcggccttg aacggggtct gatcctttcc gtaatagggt
     5161 gcataggaca cacattgcaa tttgccatca ggcggcaggg gcggcgcgac tagcgcggct
     5221 ggggtgttct gctgccagag ccagaaaccg agggcgatca ggatcgcgag gttgaacagg
     5281 atatggaacg agtggaaacg cggtgatgcc tggtcggctg ggtctttgat cggcatggcg
     5341 gcccggaa
//
LOCUS       DATMTW010000197         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_251986, whole genome shotgun sequence.
ACCESSION   DATMTW010000197 DATMTW010000000
VERSION     DATMTW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251986"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..649)
                     /locus_tag="VN084_03765"
     CDS             complement(2..649)
                     /locus_tag="VN084_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWT28605.1"
                     /translation="MKTLSLVLLSIVALQLAGCATTTNSSVSGVQRKQFLMLSEKQVE
                     NMSVQAYTQSLQEATKNKKLNTNATEVQRVRNISNKLIAQTPVFRPDAKNWKWEVNVE
                     ESDQVNAYCMPGGKIMVYTGLIDKLKTTDDELAAVIGHEISHALREHGRERMSIAAVQ
                     QVGLVGLAALASASKSPNAAAAGVQAAAQSRGRTGRATHALIVIIRWTSHFHSNP"
     gene            complement(711..>2438)
                     /locus_tag="VN084_03770"
     CDS             complement(711..>2438)
                     /locus_tag="VN084_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HWT28606.1"
                     /translation="FGILALILWSLIMVVSVKYVAFIMRADNRGEGGIMALLALATRN
                     FAGQPKKQRTIMMIGILGACMFYADGMITPAISVLSAVEGLEVAAPGLESAIVPVTLV
                     VIFLLFWMQSQGTALIGGMFAPIMLLWFSTLAVLGIINILHEPRVLYALNPYYAVKFF
                     MAMPLVAFVALGSVVLAVTGTEALYADMGHFGRKPIQLAWFALVLPALVLNYFGQGAL
                     ILHDPTSIKNPFYLLAPQWMLYPLIVLATTATVIASQAVISGAFSVSRQALQLGFIPR
                     MHVQHTSEQQKGQIYMPYVNWALMLAVMLLVVGFGSSGDLAAAYGIAVTGDMVITTLL
                     ASVVFSGVWGWSWLRTGALVALFLVVDIAFFSANVLKIPDGGWVPLLIGIVIFTLMST
                     WKKGRELVFNRLKRDAMELEPFIDAIGGHLPTRVAGTAVFMTQDPNAVPHALLHNLKH
                     NKVLHEKVILLNVKIEDIPYIDPEERNVLEVLPHNFFRLTMHYGFKDELDVPADLARC
                     QGCHIEVDAIDVSYFIGKENLIAKHESEMYYWRQKLFCGMFRNADSVTNHCKLPPNRV
                     VELGAQLTL"
BASE COUNT          494 a          694 c          773 g          477 t
ORIGIN      
        1 atcagggatt ggaatgaaaa tgtgacgtcc atcggattat aacgattaga gcgtgagttg
       61 cgcgcccagt tcgaccacgc gattgggcgg cagcttgtac gcccgcagct gccgcattcg
      121 ggctcttgga agcgctggcg agtgccgcca gcccgaccag gccgacctgc tgtacggcgg
      181 cgatcgacat gcgttcacgg ccgtgctcgc gcagggcgtg cgagatctcg tgaccgatga
      241 cggcggcgag ttcgtcgtcc gtggtcttca gtttgtcgat gaggccggtg tagaccatga
      301 tcttgccgcc tggcatgcaa taggcgttga cctggtcact ctcctcgacg ttgacttccc
      361 atttccagtt cttcgcatcc gggcggaaca ctggcgtctg cgcgatcagc ttgttggaga
      421 tgttgcgcac gcgctgcact tccgtggcgt tggtgttgag cttcttgttc ttggtggctt
      481 cctgcagcga ttgcgtatag gcctggaccg acatgttctc cacctgtttc tcagacagca
      541 tgaggaactg cttgcgctgc acgccggaga cgctggaatt ggtcgtggtg gcgcagccgg
      601 caagctgcag ggcgacgata ctcaacaaga ctagggacaa tgttttcatg gcgatcttct
      661 tgataatcag ggattggaat gaaaatgtga cgtccatcgg attataacga ttagagcgtg
      721 agttgcgcgc ccagttcgac cacgcgattg ggcggcagct tgcagtgatt ggtgacgctg
      781 tcggcattgc ggaacatgcc gcagaacagt ttctggcgcc agtagtacat ctccgattca
      841 tgcttggcga tgaggttctc cttgccgatg aagtaggaga cgtcgatcgc atccacctcg
      901 atatggcagc cctggcagcg cgcgagatca gccggcacgt cgagttcgtc cttgaagccg
      961 taatgcatgg tcaaccggaa gaagttatgc ggcagcactt ccaggacatt gcgctcctcg
     1021 gggtcgatgt aggggatatc ctcgatcttg acgttgagca ggatcacttt ctcgtgcagc
     1081 accttgttgt gcttgaggtt gtgcagtagc gcgtgcggca cggcgttcgg gtcctgcgtc
     1141 atgaataccg ccgtaccggc gacgcgggtg ggcagatggc cgccgatggc gtcgatgaag
     1201 ggttccagct ccatcgcgtc gcgcttgagg cgattgaaga ccagctcgcg acctttcttc
     1261 caggtagaca taagcgtgaa gatgacgatg ccgatcagca gcggtaccca gccaccatcg
     1321 gggatcttca gcacgttggc gctgaagaag gcgatatcga cgaccaggaa cagcgcgacc
     1381 agtgccccgg tacgtagcca gctccagccc catacgccac tgaagaccac tgaagcgagc
     1441 agggtcgtga tcaccatatc gccggtgacg gcgataccgt aggccgcggc caggtcgccc
     1501 gacgaaccga agccgaccac cagcagcatc accgccagca tcagggccca gttcacatag
     1561 ggcatgtaga tctggccttt ttgttgttcc gaggtgtgct gcacatgcat acgcgggatg
     1621 aagccgagct gcagggcttg gcgcgacacc gagaacgcgc cggagatcac cgcctgcgag
     1681 gcgatcacgg tggcggtggt cgccagcacg atcagcggat acagcatcca ttgcggcgcc
     1741 agcagataga acgggttctt gatcgaggtc gggtcatgca ggatcagcgc accctgaccg
     1801 aaatagttga gtaccagcgc cggcagcacc agcgcgaacc aggcgagctg gatcggtttg
     1861 cggccgaagt ggcccatgtc ggcatacagc gcttccgtcc ccgtcacggc cagcaccacc
     1921 gagccgagtg cgacgaaggc gaccaagggc atggccatga agaacttcac ggcgtagtag
     1981 gggttgagcg catagagcac gcgaggttcg tgcaggatat tgatgatgcc gagcaccgcc
     2041 agtgtgctga accagagcag catgatcggc gcaaacatcc caccgattag tgccgtgccc
     2101 tggctttgca tccagaacag caggaagatg acgaccagcg tcaccggcac gatggccgat
     2161 tcgagcccgg gcgcagcgac ttccagacct tccacggcgg atagcaccga gatggccggg
     2221 gtgatcatgc cgtcggcgta gaacatgcag gcgccgagga tgccgatcat catgatggtg
     2281 cgctgttttt tcggctggcc agcgaaattg cgcgtcgcca gcgccagcag cgccatgatg
     2341 ccgccttcgc cgcggttgtc ggcgcgcatg atgaatgcga cgtacttcac cgagaccacc
     2401 atgatcagcg accacaggat cagggccagg atgccgaa
//
LOCUS       DATMTW010000198         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_51613, whole genome shotgun sequence.
ACCESSION   DATMTW010000198 DATMTW010000000
VERSION     DATMTW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51613"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1109
                     /gene="queA"
                     /locus_tag="VN084_03775"
     CDS             75..1109
                     /gene="queA"
                     /locus_tag="VN084_03775"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWT28607.1"
                     /translation="MRTTDFDFDLPQELIAQFPATERGASRLLRLDGNTGAIDDRMFR
                     ELPAFLQPNDLLIFNDTRVIKARLHGQKASGGNVEVLVERILDDHAALAHVRASRAPK
                     AGSLLHLAGGQIAAVVRERSDDLFLLDFAGPETLLTLLDRHGQLPLPPYIEHAADDTD
                     EERYQTVYAREAGAVAAPTAGLHFDEAMMQRLAAHGIKIAYITLHVGAGTFQPVRVED
                     IREHKMHSELYSVSAETVRMIEATRAAGGKVTAVGTTALRALESAARSGALVAGNGET
                     DIFITPGYRFQVVERLLTNFHLPKSTLLMLVSAFAGLDHIKNAYGHAVEQRYRFFSYG
                     DAMLLERQEP"
     gene            complement(1106..2203)
                     /locus_tag="VN084_03780"
     CDS             complement(1106..2203)
                     /locus_tag="VN084_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28608.1"
                     /translation="MKKVPHLLVCISGHGFGHLAQVAPVLNALHDLSPDIRLTIRSTI
                     PQPLLRQRIRPEFNHLQEATDFGMVMRSALQVNVAASMQAYVDFHADWPAKVAAEAQR
                     LQRLAPDLLLTDVAYLPLAAADRIGLPAVAMCSLNWADIFAHYCHAMPEADRIQQEIA
                     SAYACASCFLQPTPSMPMPWLPERMVIGPLAEAGRQRRFEIERQLGLQSEDRLVLVSM
                     GGIPTRVALDRWPRLPGVKWLVQGDWSLPQARDDMHAFESLGMPFSDLLASCDLLLTK
                     PGYGAFVEAAVSGVPVLFVRRDDWPEQPYLVDWLAQVRHCQGIDPLQLERGGFVDALE
                     ALLEKTKPLPLAATGHLQAARYMLSRLAEAI"
     gene            2293..3177
                     /locus_tag="VN084_03785"
     CDS             2293..3177
                     /locus_tag="VN084_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28609.1"
                     /translation="MQLSRIFVTLLYLFILPLAHADVLPEAQVEIKIPLTKQPTTRPM
                     TIAYVPYLSRYYIADGGLAPMPGDMEIPLSKSQVHVYDAKGNYIASGKPGLDNRSIYY
                     NPNTHKLETVTYNVSSDAGFGPNTGVFAIELTETGDIKDFPTEIASFNPAFGSASTMP
                     SYDVDHNRYFAKQERSNKVFIVELGKREKVGEIALDFAAAGVQHDDLSEHFVAYTGIP
                     GEELALLDVDHKAVLVFDINGKFVGKSALPKTMKLRAQNHYNGLGYTNGMVFVYHEPE
                     GEFGTYYGFKISDKAQVN"
BASE COUNT          707 a         1028 c          900 g          639 t
ORIGIN      
        1 attttgagtc cgctgcgtct accaattccg ccatcccggc aaaagaggca gcattatagc
       61 aagcagtcaa ccctatgcgc actacagatt tcgattttga ccttcctcag gaactcatcg
      121 cccagttccc tgccaccgag cgtggcgcca gccgcctact gcggctggac ggcaacaccg
      181 gcgccatcga tgaccgcatg ttccgtgagt tacccgcttt cttgcaaccg aacgacctgc
      241 tgatcttcaa tgacacacgc gtgatcaagg ccaggctgca tggccagaaa gccagcggcg
      301 gcaacgtcga agtccttgtc gagcgcatcc tcgatgatca tgccgcactg gctcacgtcc
      361 gcgcttcgcg cgcccccaag gccggcagtc tcctccatct tgccggcggg cagatcgctg
      421 cggtcgtgcg cgaacgcagc gatgacctat tcctgctcga tttcgccggc ccggagaccc
      481 ttctgacgtt gcttgatcgc catggccagc tccccttgcc gccttacatc gagcatgcgg
      541 ccgacgacac cgacgaagaa cgctatcaga ccgtgtatgc gcgtgaggcc ggggctgtcg
      601 cggcgcccac agcggggctg cacttcgatg aagccatgat gcaacgcctg gccgcgcatg
      661 gcatcaagat cgcctacatc acgctgcatg tcggcgccgg caccttccag ccggtccgcg
      721 tcgaggacat ccgggaacac aagatgcaca gtgaactgta ttcagtcagc gcagagacgg
      781 tgcggatgat cgaagcaacc cgggctgccg gcggcaaggt cacggccgtg ggcaccacgg
      841 cgctccgggc acttgaaagc gctgcgcgta gcggcgcact ggtcgccggc aatggcgaga
      901 cggatatctt catcacaccg ggttatcgct tccaggtcgt ggagcgctta ctcaccaatt
      961 ttcacctgcc gaaaagcact ttgctgatgc tggtctcggc attcgccggg ctcgaccata
     1021 tcaagaacgc ctacggtcac gcagtcgagc agcgttaccg cttcttcagc tacggtgatg
     1081 ccatgctgct ggaacggcag gagccctaga tcgcttccgc cagccgcgat agcatgtatc
     1141 gcgccgcttg cagatgacca gtcgccgcca gcggcaatgg tttcgttttt tccagcaacg
     1201 cctccagcgc gtcgacgaat cccccacgct ccagttgtag cgggtcgatt ccctgacaat
     1261 gtctgacttg cgccagccaa tccaccagat aaggttgctc cggccaatca tcccgcctga
     1321 cgaacaatac cggcacgccg ctcactgcgg cttccacgaa agccccgtaa cccggtttgg
     1381 tcaacaacag atcgcaactt gccagcaggt cggagaatgg catacccagt gactcgaagg
     1441 catgcatgtc atcgcgtgct tgcggcaacg accaatcccc ctggaccagc cacttcacgc
     1501 caggtaatcg cggccagcga tccagagcaa cgcgcgttgg gataccgccc atgctaacca
     1561 acacaagtcg atcttccgat tgcaggccga gctgccgttc gatctcgaaa cggcgctgcc
     1621 taccggcctc tgccagtgga ccaatcacca tgcgctctgg tagccaaggc atcggcatcg
     1681 aaggtgtggg ctgcaggaaa cagctggcac aggcataggc tgaggcgatc tcttgctgga
     1741 tccgatccgc ctctggcatg gcgtgacagt aatgcgcgaa gatgtcggcc cagttgagcg
     1801 aacacatcgc caccgccggc aggccaatcc tgtctgccgc ggcaaggggt agataagcca
     1861 catcggtcag caacaggtcc ggggcgagcc gttgtagacg ctgggcctcg gcggccacct
     1921 ttgccggcca atcggcatgg aaatccacat aggcttgcat gctggcggcg acattcacct
     1981 gaagcgcaga tcgcatgacc atgccgaaat cggtcgcttc ctgcagatga ttgaactccg
     2041 gccggatacg ctgtctcaaa agtggttgcg gtatcgtgct tcgaatcgtc aagcgtatgt
     2101 ccggcgacaa gtcatgcaag gcgttgagta ccggcgccac ttgggccaga tgcccgaaac
     2161 cgtgacccga gatgcatacc agtaagtggg ggactttttt cattaatgct ttacagcggg
     2221 atgcgcttcc atacaatgga ccgtcatttt atgaccagac cgccgatcaa tagaccgtca
     2281 gaaagagaat tcatgcagct ttccagaata ttcgtcacgc tcctgtacct gttcatcctg
     2341 cctttggccc atgccgatgt cctgccggag gcccaggtgg agatcaagat cccattgacc
     2401 aagcaaccga ccacacggcc gatgaccatc gcctacgtcc cctaccttag ccgctattac
     2461 atcgcagatg gcggcctcgc gccgatgcct ggcgacatgg agatccccct gtcgaaatcg
     2521 caggtgcatg tctacgacgc caagggtaac tacatcgcct ccggcaaacc ggggctggat
     2581 aatcgctcca tctattacaa cccgaatacg cacaagctgg aaacagtgac ctacaacgtg
     2641 tcgagtgatg ccggattcgg cccgaatacc ggcgtcttcg ccatcgaact gaccgaaacc
     2701 ggggacatca aggatttccc gaccgagatc gcatccttca acccggcttt cggtagcgcg
     2761 tcgaccatgc cttcgtacga tgtcgaccat aatcgttatt tcgccaagca ggagcggtcc
     2821 aacaaggtgt tcatcgtcga acttggcaag cgcgagaaag tcggcgagat cgcactggac
     2881 tttgccgctg ccggcgttca gcacgatgac ctgagcgagc atttcgtcgc ctataccggt
     2941 atccccggtg aggaactggc attactggac gtggaccaca aggccgtgct ggtattcgat
     3001 atcaacggta aattcgtcgg caagagtgca ctgccgaaaa ccatgaagct gcgcgcgcag
     3061 aaccattaca acggactggg ttacaccaac ggcatggtgt tcgtttatca cgagccggaa
     3121 ggcgaattcg gcacctacta tggcttcaag atctccgaca aggctcaggt caattaagca
     3181 acgccagacg taaaaacggc gacgcgcggt gatccggcgt cgccgttttt acgtctggcg
     3241 ttgcttaatt gacctgagcc ttgtcggaga tctt
//
LOCUS       DATMTW010000199         6955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_252105, whole genome shotgun sequence.
ACCESSION   DATMTW010000199 DATMTW010000000
VERSION     DATMTW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252105"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VN084_03790"
     CDS             complement(<1..252)
                     /locus_tag="VN084_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28610.1"
                     /translation="MSDLQLALLALGAVIIVAVILFNWWQERKLRKLAADAFEPAVRD
                     PLLEDEFSLDTSAVPDEEPQRSASFSPTYDEAMTSSIPAI"
     gene            complement(298..3819)
                     /gene="smc"
                     /locus_tag="VN084_03795"
     CDS             complement(298..3819)
                     /gene="smc"
                     /locus_tag="VN084_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWT28611.1"
                     /translation="MRLTHLKLAGFKSFVDPTTLHIHGQRVGVVGPNGCGKSNVMESV
                     RWVLGESSAKEMRGDSMADVIFNGSGNRKAISRASVELIFDNSLGGAFDNSAGEWSQY
                     AEIAVKRVIERDKGSSYFINNTPVRRRDVADLFLGTGLGGRAYAIIGQNTISRIVEAK
                     PEELRVFLEEAAGISKYKERRRETELRLRDTRENLVRVEDIRREIDKQIARLESQAAV
                     TAEFHRLQDALKLAQGQLWLLKKRDAALQLDKAQRQVEKLVNGLEAQMAELRKSESLV
                     ETLRQQHYAATEQVQSTQTRYYEANAEVSNLEQQVRHTQEARERLTQQLQQITQQAQK
                     TDAQLLSLQQQLAQLEQQQQQSAAQQADAERSLHEMRQGLPEKERAFSAAQARHAVAQ
                     KLYFETEQNIKLESAGLDHLNRSLQEQQQRLQQLENELKTLQVPDSRELEQRQQESLA
                     QAQAITTLEQQVTDHRVQEQALITRISQTRERQLQDQRALTQAEAQIASLARLQQAMG
                     QESKLEAWLKTAGLTDAARIWQKVSIETGWETALESALGARLNALLQTSLPAPQGQRP
                     PASLVISEPGMEIAAKPNPDLRPILSLLSFRQARLAGLMQDWLAHLYLLEDDSALERL
                     RTKLRPGEALVNRAGDLYTRHSVSYHAAQSSMHGVLERQRELETLEASIPALQSAVSQ
                     STAALAEAEAALQQSRQQAQSQQQALRNATQAQHQLTLDIQRLQQQQSHMLERQRSMQ
                     AELVTLRQRIDGLRSESSAKQQALTGLEQAVSEQLTQREQSQQLRRDAETVLNEARVA
                     LLKAERMAQEKSFDSRIFVNNINEIKSKLTNSSEEKATLAVRLQETETLLEAARMDTL
                     KTNLEAALILKQTREKELADARNQMAGVEQSLQNEERTRMQNEQQLHPQRDKLEQARL
                     HAQESRIHFEQCQQGLSTVGLDEAMLEAELSASARLSEIERKVTATQQEIEALGAVNL
                     AATQELASELERKSYLDHQAADLDQAIQTLEDAIRRIDKETRTRLQNTFDEANRHFGE
                     LFTTLFGGGQARLELLGEEILDTGMQVFAQPPGKKNSTIHLLSGGEKALTALALVFAL
                     FRLNPAPFCLMDEVDAPLDDSNTERFCHLVKKMSERTQFLFVSHNKITMEMAQQLIGV
                     TMQESGVSRIVEVDIEAAMQMNAELVT"
     gene            4004..4423
                     /gene="queF"
                     /locus_tag="VN084_03800"
     CDS             4004..4423
                     /gene="queF"
                     /locus_tag="VN084_03800"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWT28612.1"
                     /translation="MSSQPSKTLETFDNPKPERDFHIHMEIPEFTCLCPKTGQPDFAV
                     LYLDYIPDQKCVELKSLKLYMWSFRDEGCFHEAVTNRILDDLVAATQPKFMRLTAKFY
                     VRGGVFTNVVAEHRKSGWQPAPRVDLTQFDTQSNIRG"
     gene            4556..4631
                     /locus_tag="VN084_03805"
     tRNA            4556..4631
                     /locus_tag="VN084_03805"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4589..4591,aa:Ala,seq:ggc)
     gene            4707..4782
                     /locus_tag="VN084_03810"
     tRNA            4707..4782
                     /locus_tag="VN084_03810"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4741..4743,aa:Glu,seq:ttc)
     gene            complement(4950..>6955)
                     /locus_tag="VN084_03815"
     CDS             complement(4950..>6955)
                     /locus_tag="VN084_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HWT28613.1"
                     /translation="GLYAFWRETLRHDHGIGILMGLPHIGHAIDALPTDARDAERWVV
                     KRLGLPEAVWAEYLESVLLTVNGWASWCAYLGWQAQLEGTEDQHLRELLAIRLAWGVL
                     LLECKDDDATRQAFSALQRSWGQAQQVLKSAEEALLIDEVWQLALEKGYQRDLATQLL
                     AKESAVAETPDVDVQAIFCIDVRSEPMRLALESVMPGMQTLGFAGFFGLPVAYTPIAT
                     QARRPQLPGLLAPAIEVTDRLQPAGMHGHENTASMEQRAQRSRRVRLAIKDQWLATSR
                     WPSAAFSFVEAAGFGYLGKLGRWLAPGRQARPRDDLEGLPGRYRAICRPWLVGLDLES
                     RVSLAVKVLHAIGLERNIAPLVLLVGHGSQSSNNAHAAALDCGACCGQTGEVNARGLA
                     QLLNEPAVREGLQARGIHIPDQTWFVAALHNTTTDEMEGFDLDLLPPVAAARWAKLQA
                     VFQQAGGKVRKARAPGLGLDPALPHDALLDQLRRRANDGAQTRPEWGLAGNAAFLIAD
                     RSKTRGVSLAGRSFLHDYKASEDTDGSILELLMTAPMLVTHWINWQYHASASDPLRFG
                     SGNKVLHNVVGGNIGVFEGNGGDLRIGLSRQSVHDGERFVHEPLRLTVVIDAPRQAIE
                     AIIGRHAVVQQLVAHRWLHLWRFDASGLERYADGAWLRVSPAI"
BASE COUNT         1279 a         2138 c         1992 g         1546 t
ORIGIN      
        1 aatcgccggg atgctggaag tcatggcttc atcgtaggtc gggctaaatg atgcgcttcg
       61 ttgcggctct tcgtcgggca ctgcgcttgt gtcgaggctg aattcgtctt ccagcaaggg
      121 gtcgcgtact gcaggctcaa aagcgtcggc agcgagcttg cgcagcttgc gttcctgcca
      181 ccagttgaag aggatgaccg ccacgatgat gacggcgccc aaggccagca aggccagttg
      241 taaatcactc atagatcgac tctttgcgat gcacggatat tattagtatg gttgaattca
      301 ggtcaccaat tcggcattca tctgcatcgc ggcttcaata tccacttcca cgatgcgcga
      361 cacccccgat tcctgcatgg tgacaccgat cagttgctgg gccatttcca tggttatttt
      421 attatggctg acgaacaaaa actgcgtgcg ttccgacatt tttttcacca gatggcaaaa
      481 acgttcggta ttgctgtcat cgagtggcgc atccacttca tccatcaggc agaaaggcgc
      541 aggattgagc cggaacaagg cgaacaccag cgccagcgca gtcagcgcct tttcgccgcc
      601 agataaaagg tggatggtgc tgttcttctt gccgggaggc tgcgcgaaca cctgcatgcc
      661 ggtatcgagg atctcctcac ccagcagctc gagccgtgcc tgaccgccgc cgaacaaggt
      721 agtgaacagt tcgccgaaat ggcggttggc ctcgtcgaag gtgttctgca aacgcgtgcg
      781 ggtctccttg tcgatccggc ggatcgcatc ttccagggtc tggatggctt gatcgagatc
      841 agccgcctga tggtcaaggt aggacttgcg ctcaagctcg ctcgcgagct cctgggtggc
      901 cgcgaggttc accgcgccca gcgcctcgat ctcctgctgc gtggcggtga ctttgcgctc
      961 gatctcactc agcctggcag aagcggatag ttcggcttcg agcattgcct catcgaggcc
     1021 aacggtcgac aaaccttgct gacactgctc gaaatggata cggctttcct gtgcatgcag
     1081 cctggcctgt tccaatttgt cgcgctgcgg atgcagttgt tgttcattct gcatgcgcgt
     1141 ccgttcttcg ttttgcagtg actgctcaac cccggccatc tgattacgtg cgtccgccaa
     1201 ttccttttcg cgggtttgtt tcagtatcaa ggccgcttcg aggttggtct tcagggtgtc
     1261 catgcgcgcg gcctcgagca gtgtttcagt ctcctgaagt cgcacggcaa gcgtcgcttt
     1321 ttcttcactt gaattcgtaa gtttgctttt tatctcattg atattattga cgaatatcct
     1381 gctatcgaaa cttttttcct gcgccatgcg ctcggctttc agtaaagcca ccctcgcctc
     1441 gttcagcacg gtctcagcat cacgtcgcaa ttgctgggat tgctcgcgct gggtcagttg
     1501 ctccgagaca gcctgctcaa gccccgtcag tgcctgctgt ttggctgagg attctgatcg
     1561 cagaccatcg atcctttgtc gcaacgtcac cagctcggcc tgcatcgatc gttgccgctc
     1621 cagcatatgt gactgctgtt gctgcagacg ctggatatca agcgtcaact ggtgctgcgc
     1681 ctgggtggca ttgcgcaggg cctgctgttg cgactgtgcc tgttgccggg actgctgcaa
     1741 cgcggcttct gcttcggcaa gggctgcggt gctttggctg acagctgact gcagggcggg
     1801 gatcgaggct tccagggttt caagctcacg ctggcgctca agcacgccgt gcatcgacga
     1861 ctgtgcagcg tgataactga cgctgtgccg ggtgtataga tcgccggcac gattgaccag
     1921 cgcttcgccc gggcgtaatt tcgtccgaag acgctcaagc gcgctgtcgt cttccagcag
     1981 atataggtgc gccagccagt cctgcatcag cccggcgagc cttgcctgcc tgaagctcaa
     2041 caaggaaagg atcggccgaa ggtcaggatt gggcttcgct gcgatctcca tccccggctc
     2101 gctgatgacg agcgaagctg gcgggcgttg cccctggggc gcaggcagcg atgtctgcaa
     2161 cagggcattg agccttgctc ccagcgccga ctcgagtgcg gtctcccacc ccgtttcgat
     2221 actgactttt tgccagatgc gcgccgcatc cgtcaggcct gccgtcttga gccaggcttc
     2281 gagtttggat tcctggccca tggcctgttg caaccgcgcc agtgaggcga tctgcgcttc
     2341 ggcctgggtc agcgcgcgct ggtcctgcag ttgccgctca cgcgtctggc tgatgcgcgt
     2401 gatcagcgcc tgctcctgaa cccggtgatc cgtgacttgc tgttcgagcg tagtgatcgc
     2461 ctgtgcttgc gccagcgatt cctgctgccg ttgttcgagt tcgcgggaat ccggcacctg
     2521 caaggtcttc agctcgtttt ccagctgctg caggcgttgc tgctgctcct gcaggctgcg
     2581 attcaggtga tctagaccag cgctttcgag tttgatgttc tgctcggttt cgaaatacag
     2641 cttttgcgcg acggcatgac gcgcctgggc ggcgctgaat gcacgttcct tctctggcag
     2701 gccttgccgc atctcatgca gggatcgctc ggcatcagcc tgttgggcgg ctgattgctg
     2761 ctgctgttgc tcaagttgcg ccagctgttg ctgcaaactc agcagctgtg cgtcggtctt
     2821 ctgggcctgc tgggtgattt gctgcaactg ctgggtcagc cgctcacggg cctcctgcgt
     2881 atgccggact tgctgttcaa ggttggaaac ctcggcattg gcttcgtagt agcgcgtttg
     2941 cgtgctttgt acctgctccg tcgccgcata atgttgctga cgcagcgtct cgaccaggct
     3001 ttcgctcttg cgcaattcag ccatctgcgc ttccagccca ttgaccagct tttccacttg
     3061 ccgctgcgcc ttgtcgagtt gcaaagcggc atcgcgcttt ttgagcagcc ataactgccc
     3121 ctgcgcaagc ttcagggcat cctgcagtcg atgaaactcc gccgtgacgg cggcctgcga
     3181 ttccagacgt gcgatctgct tgtcgatctc acgccggatg tcctccacac ggaccaggtt
     3241 ctctcgcgtg tcgcgcagcc gtagctcggt ctcgcgtctg cgttccttgt atttcgagat
     3301 tcccgctgct tcctcaagga acacgcgcag ctcttccggt ttggcttcaa cgatacgcga
     3361 aatggtgttc tggccgatga tggcgtaggc tcgcccgcca agcccagtgc ccaggaacag
     3421 gtcggcaacg tcgcggcgac gcaccggggt attgttgatg aaataactgg agcccttgtc
     3481 gcgctcgatg acgcgcttga cggcgatctc agcatattgc gaccattcgc cggcgctgtt
     3541 gtcgaaggcg ccccccaggc tattgtcgaa gatcaactcg acactggcgc gcgagatggc
     3601 tttgcggttg cccgaaccgt tgaagatgac atccgccatc gaatcgccgc gcatctcctt
     3661 tgcgctcgat tcgcccagca cccagcgtac cgattccatg acgttggatt tgccgcagcc
     3721 attggggcca acaacgccga cacgctggcc atggatatgc agcgtagtcg gatcgacaaa
     3781 ggatttgaaa ccagcgagct taagatgagt gaggcgcaat gggtcagatc aatgggttag
     3841 aagatgatgt tcttggggca tcaagcatcc cggcatcatt ttaagtatcc aagattcatc
     3901 aagtggttga gcaagcttgt ataatacggg attcacatcg gatgccgtct gcagaaccaa
     3961 tccaggccgc atcgctctta ttacgattta tacggtagcc cttatgtcta gtcaacccag
     4021 caaaacgctc gaaacctttg acaatccgaa accggagcgc gatttccata ttcacatgga
     4081 gattcccgag ttcacctgcc tctgccccaa gacaggccaa ccggatttcg ccgtgctcta
     4141 tctcgactat atccccgacc agaaatgcgt cgagctcaag agcctcaagc tctatatgtg
     4201 gtcgttccga gacgaaggct gcttccatga ggccgtcacc aaccgtatcc tcgatgacct
     4261 ggtcgccgcc acccagccga agttcatgcg tctcaccgcc aagttctatg tacgcggcgg
     4321 cgttttcacc aacgttgtcg ccgagcaccg caaatccggt tggcagccgg cgcctcgcgt
     4381 cgacctcacc cagttcgata cgcaatccaa catccgcgga tgagcctcgg cgccccgcga
     4441 cagattgtcc tgtcgcatca ggcgactatc tgcggacggt cggcagtcgc tgcgatatcg
     4501 gtggaaatag aggttgacaa gcgccctgcc cgtctttaag attgcgcctc ttttcggggg
     4561 tatagctcag ctgggagagc gcttgcatgg catgcaagag gtcagcggtt cgatcccgct
     4621 tacctccacc agtaacgatt ctgattgaat ttttgctgaa gaaaagacga aagttgtagt
     4681 aaaatacgca tcgttaaatg ttccgggtcc ccatcgtcta gaggcctagg acacctccct
     4741 ttcacggagg cgaccggggt tcgactcccc gtggggacgc caacaataac acagcaaaaa
     4801 accgcttaag tgcggttttt tttcgcctac cgttttcacc tatcgataac cgccgccagc
     4861 ggcaccctga tgcaacggga ggcacggagg atgcttaaac caccgtgccc cgtacagtcg
     4921 ccctccccga ttgatttttt tctgagcgtt tatatcgccg gcgataccct tagccacgcg
     4981 ccatctgcat agcgttccaa tccggacgca tcgaagcgcc atagatgcag ccagcgatgc
     5041 gcgaccaact gctggacgac agcatgcctg ccgatgatgg cttcaatcgc ttggcgagga
     5101 gcatcgatca cgaccgtgag ccgcaagggt tcatgcacga accgttcgcc atcatggacc
     5161 gattggcgcg atagtccgat ccgcaggtcg ccaccattgc cctcgaacac cccgatattg
     5221 ccacctacca cgttatgcag caccttgttg ccgctaccga accgcagcgg gtcgctggcc
     5281 gaggcatggt attgccagtt gatccagtgc gtcaccagca tgggcgcggt catcaacaat
     5341 tccaggatgc tgccgtcagt atcctcgctg gctttgtagt cgtgcaggaa actgcggccc
     5401 gccagcgaca cgccacgcgt tttgctgcgg tcggcgataa ggaatgcggc attgcccgcc
     5461 aagccccatt ccggccgcgt ttgggcgccg tcgttagcgc ggcggcgcaa ctgatccaaa
     5521 agcgcatcgt gaggcaacgc tggatccagc ccgaggccag gcgcacgggc tttgcgtacc
     5581 ttgccgccag cctgctggaa aacagcctgc agtttcgccc agcgcgccgc ggcaaccggt
     5641 ggcaggagat ccagatcgaa gccttccatc tcgtccgtgg tcgtgttgtg caaggcggcc
     5701 acgaaccagg tctgatccgg gatatggatg ccgcgcgctt gcaacccttc tctgacggcc
     5761 ggctcattga gcaattgcgc caagccgcgc gcattgactt caccggtctg gccgcaacag
     5821 gcgccgcagt ccagcgcagc ggcatgggcg ttgttgctgg actgactgcc gtggccaacc
     5881 agcaacacaa gtggcgcgat attccgctct agaccgatgg cgtgcagcac tttgacggcc
     5941 agggagaccc tggattcgag gtcgaggccg accagccagg gcctgcagat ggcacggtaa
     6001 cggcccggta aaccttccag atcatcccgg ggtctcgcct gcctgcccgg ggcaagccag
     6061 cggcccagtt tgccgaggta accgaacccc gctgcttcca cgaaggagaa cgcagcgctg
     6121 ggccagcgac tggtggcaag ccactgatcc ttgatggcga gcctgacgcg acgcgaacgc
     6181 tgcgcacgct gttccatcga tgccgtgttc tcgtgaccgt gcattcccgc cggctgcaga
     6241 cgatcggtca cttcgatggc aggcgcgagt aatcccggaa gctgaggcct gcgcgcctgc
     6301 gtggcgatcg gcgtgtaggc gaccggcagg ccgaagaagc cggcgaaccc cagcgtctgc
     6361 atccccggca tcacggattc gagcgccagc cgcatcggtt cgctgcgcac gtcgatgcag
     6421 aagatcgcct gcacgtcgac gtcaggtgtc tcggctactg cgctttcctt ggccagcagc
     6481 tgtgtggcca ggtcacgctg gtagcctttt tccagcgcca gttgccacac ttcgtcgatc
     6541 agaagcgctt cttctgccga cttcagtacc tgctgcgctt gcccccagga ccgttgcaac
     6601 gcgctgaacg cctgccgggt cgcatcgtca tccttgcatt ccagcagcaa cacgccccag
     6661 gccagccgga tcgccagaag ctcacgtaaa tgctggtctt ccgtaccttc aagttgcgcc
     6721 tgccagccca gataggcgca ccaggaggcc caaccgttga cggtgagcag cactgattcc
     6781 agatattccg cccagaccgc ctcgggcagg cccagccgct tcactaccca tcgctccgca
     6841 tcgcgcgcgt ctgtgggcaa ggcgtcgatg gcgtgaccga tatgcggcaa gcccatgagg
     6901 atgccgatgc catggtcatg ccgcaaggtc tcgcgccaga aggcatagag gccct
//
LOCUS       DATMTW010000200         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_521218, whole genome shotgun sequence.
ACCESSION   DATMTW010000200 DATMTW010000000
VERSION     DATMTW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521218"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..762)
                     /locus_tag="VN084_03820"
     CDS             complement(199..762)
                     /locus_tag="VN084_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HWT28614.1"
                     /translation="MRTEIHVHGYLELVEGVSRRQVEHALRPLLDDLELDQLNAIKSL
                     EPDQPGFHFDDRSWTLDICCTLEAGPHFVTILEAAMHALGPLVENAAPIEISTYHEDG
                     RDEFQLLFVGPTPAAILEAQRREMLADIEDLLARQFNPQAVNEVLTLVDRLFRAPANM
                     LSASQAPHGSDDLPITMPSPPGRRRLH"
     gene            complement(880..1794)
                     /locus_tag="VN084_03825"
     CDS             complement(880..1794)
                     /locus_tag="VN084_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWT28615.1"
                     /translation="MVFSPRQKMTGVFFAFIAAIGFSAKAVLVKLAYIDHVDAITLLA
                     LRMAFSLPFFLLAAWLANRHQQHAAMTRRDWWAVGVFGFTGYYLASFLDFLGLQYISA
                     GLERLILFLYPTMVVLISALWFRHRISKRVIAALVLSYVGIAFAYMHDMQMQQPGLLL
                     GSALVFASALAYAVYLIGAGHTIARIGGTRFTAYAMTVACVAALLQFALTNALSDLAQ
                     PARVYWLSIAMALFSTVMPAFVLAAAIRRIGSMQTSIIGSIGPVSTIYLAYVFLGEAM
                     SAVQMLGSLLVLAGVLMISLRKQTPAQP"
     gene            complement(1836..>2291)
                     /locus_tag="VN084_03830"
     CDS             complement(1836..>2291)
                     /locus_tag="VN084_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWT28616.1"
                     /translation="IGDVHLAQDVSVWPTVVIRGDVNSIHIDAGSNVQDGSVLHVNHR
                     SADAPAGAPLHVGRYVTVGHHVILHGCTIEDECLIGMGSIVMDKAVIQRQVLVGAGSL
                     VPEGKVLERGHLYLGRPVKQIRRLTEAELRHFMYSAQNYIALKNEYLGG"
BASE COUNT          494 a          688 c          711 g          398 t
ORIGIN      
        1 gcggctggct catgctggaa catggctacg atcaggcaag cagggtcggc gagctgttgc
       61 agcgcgccgg attttcggca ctcgggcagg cgcgtgacct ggcaggtatc gtccgcgtca
      121 gctacggcca gctgacctag ctggcatcaa aggaataacg gggtaacgac ggcgagagaa
      181 cgggacgcag cggcgcgctc agtgcagccg acgacgtcct ggcgggctcg gcatggtgat
      241 ggggagatcg tcgctaccat gcggcgcctg gctggcagac aacatgttgg caggcgcacg
      301 gaacagacga tcgaccaggg tcaacacttc gttcaccgcc tgcggattga actgtcgggc
      361 cagcagatcc tcgatatcgg cgagcatttc gcgccgctgc gcctcaagga tcgccgccgg
      421 cgtggggccg acgaacagca gctggaattc atcgcggccg tcttcgtgat acgtgctgat
      481 ctcgatcggc gcggcattct cgaccagcgg ccccagcgca tgcatagcag cctccagtat
      541 cgtgacgaaa tgcggccctg cctccagcgt gcaacagata tcgagcgtcc agctccggtc
      601 atcgaagtgg aatccaggct ggtccggctc aaggcttttt atcgcgttca gctgatcgag
      661 ttcgagatca tcgagcagtg ggcgcaatgc atgttccacc tgccgccggc tgacgccttc
      721 caccagttcc agatagccat gcacatggat ttcagtgcgc acaagattcc ttcgttgaga
      781 tgaggcgctc attataaccg agtgcccagc gacgcttcgc gctaccgcat ggatcaggac
      841 cgtgcgtcgc ggaagcgtcg tatatcccct agaacgatat cagggctgcg ctggcgtctg
      901 ttttctgaga ctgatcatca acacccccgc cagcaccagc agcgacccca gcatctgcac
      961 ggccgacatc gcctcgccca ggaacacgta agccaggtag atggtcgaga ccggcccgat
     1021 ggaaccgatg atcgaggtct gcatcgagcc gatgcgccgg atcgcggctg ccagcacgaa
     1081 cgcaggcatg acggtcgaga acagggccat ggcgatactc aaccagtaga cccttgcggg
     1141 ttgcgcgaga tcggatagcg cgttggtcag cgcgaattgc aagagggcgg cgacgcaagc
     1201 gaccgtcatg gcataggcgg tgaagcgcgt cccgccgata cgggcaatgg tatgcccggc
     1261 accgatgaga tagacggcat aggccagggc gctggcgaat accagcgccg acccaagcaa
     1321 caggcctggt tgctgcatct gcatatcatg catgtaggca aaagcaatac cgacgtaact
     1381 cagcaccagc gcagcgatga cgcgcttgct gatcctgtgg cggaaccata gcgccgagat
     1441 cagtaccacc atggttggat acaggaacag gatcaggcgt tcgagaccgg cactgatgta
     1501 ttgcaggcca aggaaatcca ggaagctcgc caagtagtat cccgtgaagc cgaagacccc
     1561 cacggcccac cagtcacgcc gcgtcatggc cgcatgctgt tgatggcggt tggcgagcca
     1621 cgccgccagc aggaagaacg gcagggagaa cgccatgcgc agcgccagta gcgtgatggc
     1681 atccacatgg tcgatatagg cgagcttgac cagtaccgcc ttggcggaaa agccgatcgc
     1741 ggcgatgaag gcaaagaaga cgccagtcat tttctgacgg ggggagaaca ccataatgta
     1801 accctgtgga tgtgtggttc tggagattcg gcggattagc cgccgagata ttcgttcttg
     1861 agcgcgatat agttttgcgc agaatacatg aagtgccgga gctccgcctc ggtcagcctg
     1921 cggatctgct tcaccggcct cccgaggtaa agatgcccac gctccagcac cttgccttcc
     1981 ggcaccagac tgcccgcgcc gaccagcacc tgccgctgga tgaccgcctt gtccatgacg
     2041 atgctgccca tgccgatcag gcattcatcc tcgatggtgc agccatgcag gatgacgtga
     2101 tgcccgacag tgacgtaacg gccgacatgc aagggagcgc cggccggggc atctgccgaa
     2161 cgatggttga catgcagcac cgagccgtcc tgcacattgc tgcctgcgtc gatatggatg
     2221 ctattgacat caccgcggat caccacggtg ggccacaccg agacatcctg cgctagatgg
     2281 acgtcgccga t
//
LOCUS       DATMTW010000201         9770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_252303, whole genome shotgun sequence.
ACCESSION   DATMTW010000201 DATMTW010000000
VERSION     DATMTW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252303"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1510)
                     /gene="glgA"
                     /locus_tag="VN084_03835"
     CDS             complement(53..1510)
                     /gene="glgA"
                     /locus_tag="VN084_03835"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HWT28617.1"
                     /translation="MRTLFVSSEAYPLIKTGGLADVSGSLPAALRALGADVRLLVPGY
                     PAVLQKLLEPHHIAMIEDLPVIGSAELIMGEMPGTKVPVMAIVAPSLYERTGGPYVDE
                     TGLDWADNPLRFGILSKIAAILSTAQSPLIEQWLPDIVHCNDWQSGLTPAFMHYMGAQ
                     RAKSVLGIHNLAFQGCFSAEWIGMLGLPPECFQINGVEYHGQLSFLKAGIYYSDSITT
                     VSPTYAHEIQTEAFGFGLQGLLRTRGHEIRGILNGIETQEWDPANDPHIAVHYDAKHL
                     AGKKAVKAALQSQLGLYADPAAPLLGVVSRLTYQKGLDSFLSIAEPLLHKGCQIALLG
                     GGEATLEQGFRELGQQYPLQVSTNIGYNEALSHQIMAGTDIFIMPSRFEPCGLNQMYG
                     LRYGTPPVVTRTGGLADSVIDTNEATLKAGSANGFVIDQADPQQLLATIQRAIGYLQD
                     EKTWRRIQRNGMQLDLGWTHSARDYMSLYQELVDG"
     gene            1563..2045
                     /gene="crcB"
                     /locus_tag="VN084_03840"
     CDS             1563..2045
                     /gene="crcB"
                     /locus_tag="VN084_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWT28618.1"
                     /translation="MRQGWPALNISIYFIILLWSLPIKAASTTRRWHMGWGGFVAVGV
                     GAAAGAWMRWGLSVLFNALVPALPLGTLIANLSGGYMMGLAMGVIASNITLSPELRLL
                     LTTGFLGGLTTFSTFSAEAAALLSRGQYGWASAHVLLHVCGSLTLTILGILTIQLLRS
                     "
     gene            2048..2371
                     /locus_tag="VN084_03845"
     CDS             2048..2371
                     /locus_tag="VN084_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HWT28619.1"
                     /translation="MQGVYLKLFVPEGLRHHHELAYEWILQQAQRLGIPGGSALRALA
                     GYGRHGRLHEQHFFELAGDMPIILEFFASETAVTSLLSLLREEKLSLFYIRTAAEGGL
                     TDALT"
     gene            2511..2894
                     /gene="rpsF"
                     /locus_tag="VN084_03850"
     CDS             2511..2894
                     /gene="rpsF"
                     /locus_tag="VN084_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWT28620.1"
                     /translation="MRHYEVVFIVHPDQSEQVPAMIERYRTLVTSNGGAIHRLEDWGR
                     RQLAYPIQKIHKAHYVLMNIECNQTVLDELEHAFKFNDAVLRHLTVSVDEAVVAPSPM
                     MKEEKSKSLLEKDAAPAPAAEEASA"
     gene            2895..3185
                     /gene="priB"
                     /locus_tag="VN084_03855"
     CDS             2895..3185
                     /gene="priB"
                     /locus_tag="VN084_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HWT28621.1"
                     /translation="MNRLVVSGEVVQIDPLRYTPAGLPLLGFVIRHQSEQVEADMPRK
                     VECEVSVIAIGKIAEQAKAIQMASQVKLAGFLAKRSLKSPQLVLHLNAFEII"
     gene            3198..3425
                     /gene="rpsR"
                     /locus_tag="VN084_03860"
     CDS             3198..3425
                     /gene="rpsR"
                     /locus_tag="VN084_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWT28622.1"
                     /translation="MARDMFKRRRYCRFSAEGIKQVDYKDVDLLKDFITENGKIIPAR
                     ITGTKARYQRQLTVAVKRARFLALLPYTDKH"
     gene            3439..3885
                     /gene="rplI"
                     /locus_tag="VN084_03865"
     CDS             3439..3885
                     /gene="rplI"
                     /locus_tag="VN084_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWT28623.1"
                     /translation="MQVILLEKVVNLGNLGDVVKVKDGYGRNFLIPQGKAKRATEANK
                     AEFAARRAELEKQQAELLAAAQKRAEKLAGYLVQVTQKAGVDGRLFGSVTNFDIAESL
                     TAQGFEVSKSEVRMPNGPLKTIGDHPVSIALHHDVVVDITVSVLGE"
     gene            3994..5367
                     /gene="dnaB"
                     /locus_tag="VN084_03870"
     CDS             3994..5367
                     /gene="dnaB"
                     /locus_tag="VN084_03870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWT28624.1"
                     /translation="MADDLLESLKLPPHSVEAEQSVIGGLLLENEALDKIGDILNPDD
                     FYRHDHRLIYQHIAKLIEHNKPADIVTVAESLESSAELSGVGGIAYLGALAQNTPTAA
                     NIRRYAEIVHERAVMRKLVEVGSDIAESAYSPQGRDAQQLLDEAEAKIFHIAEGGKRS
                     TQGFLDIKVLLPQVADRIDQLYQRDNPDAVTGIPTGYTDLDEKTSGLQPGDLIIVAGR
                     PSMGKTAFSLNIAENVALDTGLPVAVFSMEMGATQLATRMIGSVGRLDQHKMRNGKLD
                     DEDWVRLTTALGKLNDAPVYIDEGAGLSSFDVRARARRLHRQCGKLGLIVVDYLQLMT
                     APSGRQGENRATEISEISRSLKALAKELDVPVIALSQLNRGLEQRPDKRPVMSDLRES
                     GAIEQDADLILFIYRDEVYNPESTDKGTAEIIIAKQRNGPIGKVRLTFIGAHTRFENF
                     VNAGASY"
     gene            5372..6463
                     /gene="alr"
                     /locus_tag="VN084_03875"
     CDS             5372..6463
                     /gene="alr"
                     /locus_tag="VN084_03875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWT28625.1"
                     /translation="MRPIQATIDLGALRHNLNVVRQHAPNARVMAVIKANGYGHGLLH
                     VAQGLQSADGFAVLNIEEAIALREAGYRQRILLLEGFFTDDEIALAASHDITVVLHAD
                     WQLDLLARQPQGIRLPVFIKLNTGMNRLGFMPAALTEALARIDALPMIDDITLMTHFA
                     TADEPRGIAEAHALFEQATAGLAYPRSMANSATILRYPQAHADWVRPGIMLYGASPVS
                     GTPAAQFGLRPAMALTSELIAVQTLQPGDSVGYGMRYTAEKPTRIGIVACGYADGYPR
                     HAGLCAAGERQPGAPIAVAGKLTHTVGRVSMDMLFADITDIPEADIGSPVELWGAQVP
                     VDAVAEASGTVGYELLCALAPRVPVRVID"
     gene            6463..7821
                     /gene="radA"
                     /locus_tag="VN084_03880"
     CDS             6463..7821
                     /gene="radA"
                     /locus_tag="VN084_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWT28626.1"
                     /translation="MAKQKTVYTCTECGGQEPKWQGQCPNCGQWNTLVETVAEPVTAN
                     RYAALAQPTGLQKLAEVEAAEVPRHPTAISEFDRVLGGGLVPGGVVLIGGDPGIGKST
                     LLLQTLCHVGRQRKALYISGEESAQQIAMRAKRLGLDASPIDLLAEIQLEKILATLQN
                     HRPDVAVIDSIQTVYSEALQSAPGSVAQVRECSAQLTRVAKQLGITVILVGHVTKEGA
                     LAGPRVLEHIVDTVLYFEGDPNSSFRLIRAFKNRFGAVNELGVFAMTEKGLREVSNPS
                     ALFLSHHTEQVAGSCITVTQEGTRPLLVEVQALVDEAHAPNPKRLCVGLEQNRLAMLL
                     AVLHRHAGVACYDQDVFVNAVGGVRISEPAVDLAVLLAIVSSLKNKALDSKLIVFGEV
                     GLAGEVRPVQRGQERLKEAAKLGFTHAIVPKSNAPRQPIKGIEIFAVDRLEQALQKAR
                     EI"
     gene            7824..8504
                     /locus_tag="VN084_03885"
     CDS             7824..8504
                     /locus_tag="VN084_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWT28627.1"
                     /translation="MQTAAWQSASPTRSSVIFDLDGTLIDSSASILRSFEAAFDKIGV
                     IARQAFTSEVIGPPLIPTLQRLAGSDDPALIQALATAFKVEYDSHAYQAATVFDGIPE
                     LLASLGQRGVQMYIATNKRILPTRKILAHLGWQEHFAGIYGLDYFEPAAASKTEMVTR
                     IIERHALTPELTLFVGDRIEDGIAAEGNGLDFAMVTWGYVDDTQGERSDNWQYFDDPH
                     ALGIYITG"
     gene            complement(8587..9342)
                     /locus_tag="VN084_03890"
     CDS             complement(8587..9342)
                     /locus_tag="VN084_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HWT28628.1"
                     /translation="MQAYQAPPDLLHTRVILVTGAGQGLGRAAAVAFARHGATVILLG
                     RDVRKLERVYDEIAAAGYPEALIFPLNLKDAQETDYQAMAEGIYQQLGRLDGILHNAA
                     HFDNLSPLELQQASQFETMLKVNLIAPFALTKACLPLLRRAPQATVLFTSSTSGQHAK
                     AFWGAHGVSKAAVVHMAQTWAQELGTPNKLQMNIVVPGPVQSPQRLKSHPGEVPQAWP
                     EAERLMPAYLYLMGPDNVTPSGEVIDLTLATHL"
     gene            complement(9391..9666)
                     /locus_tag="VN084_03895"
     CDS             complement(9391..9666)
                     /locus_tag="VN084_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWT28629.1"
                     /translation="MIARQLRIHGRVQGVGFRQSLQASATAAGLEGWVRNRRDGTVEA
                     VLSGDDARVQAVIDWTYQGPGLAKVVHVDIRECEVPKVVGFAILPTV"
BASE COUNT         1945 a         2860 c         2974 g         1991 t
ORIGIN      
        1 atgaaaaaag cccgggatac ccgggctttt ctgttttgcg acgacgcatc gatcacccgt
       61 cgaccagttc ctggtagaga gacatatagt cacgcgcgct gtgcgtccag cccaggtcca
      121 gctgcatgcc gttgcgctgg atcctgcgcc aggtcttttc atcctgcagg tagccgatgg
      181 cgcgttggat cgttgccagt agttgctgtg ggtcggcctg gtcgatgacg aagccgttgg
      241 cgctacccgc cttgagtgtc gcctcattgg tgtcgatgac ggagtccgca agcccgccgg
      301 tacgcgtgac gacaggcggg gtgccgtagc gtaagccgta catctggttc aaaccgcagg
      361 gttcgaagcg cgacggcatg atgaagatgt cggtgcccgc catgatctgg tgcgaaagcg
      421 cctcgttgta gccgatattg gtgctgacct gcagcggata ttgctgacca agttccctga
      481 acccctgttc cagggttgct tcgccaccac cgagcaatgc gatctggcag cctttgtgca
      541 acagtggttc tgcgatactg aggaagctgt cgagaccttt ctgataagtg aggcgactga
      601 cgacgccaag taacggcgcg gcagggtcgg cgtagaggcc gagttgcgac tgtagtgcgg
      661 ccttgaccgc ttttttgccg gccagatgtt tcgcatcgta atgcacggcg atatgggggt
      721 cgttggccgg gtcccattcc tgggtctcga tgccgttcag gatgccgcgt atctcatggc
      781 cgcgggtgcg caacaggccc tgcaggccaa acccgaaagc ctcggtctgg atctcgtggg
      841 cataggtcgg gctaacggtc gtgatgctgt cgctgtagta gatgccagcc ttgaggaagg
      901 acagctggcc atggtactcg accccgttga tctggaaaca ttcgggcggc aggcccaaca
      961 tgccgatcca ttctgccgag aagcaacctt ggaacgcgag attatggatg ccgagcacgg
     1021 atttcgcgcg ttgcgctccc atgtagtgca tgaaggcggg cgtcagaccg ctctgccagt
     1081 cgttgcagtg gacgatgtcg ggcagccatt gctcgatgag tgggctttgc gccgtgctga
     1141 ggatcgccgc gattttggac aagatgccga aacgcagcgg attgtcggcc cagtcgagcc
     1201 cggtctcgtc gacataaggg ccgccagtgc gttcatagag gctaggcgcg acgatcgcca
     1261 tcaccggcac cttggtgccc ggcatctcgc ccatgatcag ttcagcgctg ccgatcactg
     1321 gcagatcttc gatcatggcg atatggtgag gctccagcag tttctgcagg acggccgggt
     1381 agcccggcac caataacctg acatcggcgc ccagggcccg cagggctgcc ggcagtgagc
     1441 cgctgacgtc ggccaggccg ccggtcttga tgaggggata cgcttcggaa ctgacgaata
     1501 gggtacgcaa ggaaagactc ttttattgat gttcggctga ttattgggat ggagcggttt
     1561 gtatgcgcca agggtggccg gcgttgaata tctccatcta cttcatcata ctgttgtggt
     1621 cactgccaat caaggcggca tcgacaacaa ggagatggca tatggggtgg ggagggtttg
     1681 tcgcggtcgg cgtcggcgca gcagccggcg catggatgcg ctggggattg agcgtgctat
     1741 tcaacgcgct ggtacctgcg ttgccgctgg gcacgctgat cgcgaacctg agcggtggtt
     1801 acatgatggg gctggccatg ggcgtgatcg cgtcgaacat caccttgtcg ccggagttgc
     1861 gtttgctgct gaccactggt tttctcggcg ggttgaccac cttttccacc ttctctgctg
     1921 aagccgccgc cttgctcagt cgtggccagt atgggtgggc ctccgcgcat gtgctgctgc
     1981 atgtctgcgg ctcgctgacg ctgaccattc tgggcatcct gaccattcaa ctgctgagga
     2041 gctgaccatg caaggcgttt atctcaagtt attcgttccc gaggggttgc gccatcatca
     2101 tgagctcgcc tatgagtgga tcctgcagca ggcccaacgc ctcggcatcc ccggcggttc
     2161 cgcgttgcgc gcattggcag gctatggtcg gcatgggcgc ctgcatgagc agcatttctt
     2221 tgagctggcg ggcgatatgc ccatcatcct cgagttcttt gcctctgaaa ccgcggtcac
     2281 cagcctcctt tccctgcttc gcgaagaaaa gctgtcgctg ttctacatca ggacagctgc
     2341 ggaaggcggg ctgaccgacg cgctgacctg agccagcagg ctcgtcgggc ttggcttgat
     2401 tcacgaaagt gtttggttta ttgggtgaat ttcatttata atcgcgggtt ttccaccttg
     2461 cctcaccgtc aacgcatagc gggaggctac tttatccaaa aggagaatat atgcgccatt
     2521 atgaagtagt gttcatcgtc catccggacc agagtgaaca agtgccagcg atgatcgaac
     2581 gttatcgcac actggtgacc tctaacggcg gtgccatcca ccgtcttgaa gactggggtc
     2641 gccgccaatt ggcatacccg atccagaaga tccacaaggc acattacgtg ctgatgaata
     2701 tcgagtgtaa tcaaaccgtt ctcgatgagc ttgagcatgc attcaagttc aacgacgccg
     2761 tgctgcgtca cctgaccgtc agcgtcgacg aagctgtcgt tgcgccatcc ccaatgatga
     2821 aggaagagaa atccaagtcg ttgctggaaa aggatgcggc accagcgcca gccgctgagg
     2881 aagcatcggc ttaattgaat aggctggtcg tatccggcga agtggtgcag atcgatccac
     2941 tacgctatac gcccgcaggt ttaccgttgt tgggttttgt catacggcat caatcggagc
     3001 aggtcgaagc cgatatgccg cgcaaggttg agtgtgaggt gtcggtgatc gccataggca
     3061 agatcgccga gcaagccaag gccatccaga tggcgagcca ggtcaagctg gcaggtttcc
     3121 tcgccaaacg cagcctcaag agcccacaac tggttttaca cttaaacgca tttgagatta
     3181 tttaaaggat tcgaatcatg gcacgtgata tgttcaaacg ccgccgctac tgccgtttct
     3241 cggcagaggg catcaagcag gtcgattaca aggatgtgga cctgttgaaa gatttcatca
     3301 ccgagaacgg caagatcatc ccagcccgta tcaccggtac caaggcacgc taccagcgtc
     3361 aactgacggt cgcagtcaag cgcgcccgtt tcctggccct gttgccatac actgacaagc
     3421 actgagaggg gacacgtcat gcaagtaatt ctgttggaaa aagtcgtgaa cctgggcaac
     3481 ctcggtgacg tcgtcaaggt aaaggacggt tacggccgta acttcctgat cccgcaaggc
     3541 aaggccaagc gcgcaaccga ggccaacaag gctgagtttg ctgcacgccg cgcagagctg
     3601 gagaagcaac aagctgaact gctggctgct gcccagaagc gtgctgagaa gctggctggc
     3661 tatctggtgc aagtgaccca gaaagctggt gtggatggcc gtctgttcgg ttctgtcacc
     3721 aactttgata tcgccgagtc cctgacggct caaggcttcg aagtgtcgaa gtccgaagta
     3781 cgcatgccga atggtccttt gaagaccatt ggtgaccatc cggtcagcat cgcgctgcac
     3841 catgatgtcg tcgtcgatat cacggtgtcc gtactgggcg aataagcccc gcgggatacg
     3901 ctgttaaaaa aggctgctgc ggcagccttt tttattgtcc gcgaaattca aaagcggcag
     3961 gtcatgcccg gttttccgtt gttataatgc ggcatggctg acgatctact tgaatccctg
     4021 aaactccccc cacactccgt cgaagccgag caatcggtga tcggcggcct gctgctggag
     4081 aacgaagcac tcgacaagat cggtgacatc ctcaatccgg acgattttta ccgtcacgat
     4141 catcggctca tctatcagca catcgctaaa ctcattgagc ataacaagcc cgccgatatc
     4201 gtcacggtgg cagagtcgct ggagtcgagc gccgagcttt ctggtgttgg cggcatcgcc
     4261 tatctcggcg cgctggcgca gaacacgccg accgccgcga acatccgccg ctatgccgaa
     4321 atcgtgcatg aacgtgccgt catgcgcaaa ctggtggagg tcggctcgga tatcgccgag
     4381 agcgcctata gcccgcaggg gcgggatgcc cagcagttgc tggacgaagc tgaggccaag
     4441 atctttcaca tcgccgaagg cggcaagcgc agtacccagg gcttcctcga tatcaaggtg
     4501 ctgctgccgc aggtagcgga tcgcatcgac cagctttacc agcgcgacaa cccggatgcc
     4561 gtcaccggca ttcccacggg ttacacggac ctggatgaaa agacttccgg cctgcagcct
     4621 ggcgatctca tcatcgtcgc cggccggcct tccatgggca agacggcgtt ctcgctcaat
     4681 atcgctgaaa acgtcgcact cgacaccggc ttgccggtcg ccgtgttctc catggaaatg
     4741 ggcgcgaccc agttggcgac ccggatgatc ggctcggttg gccgtctgga ccaacacaag
     4801 atgcgtaatg gcaagctgga tgacgaggac tgggtgcgcc tgaccactgc gctcggcaaa
     4861 ctcaatgacg cgccggtgta tatcgacgaa ggcgctggcc tcagctcatt cgacgtgcgt
     4921 gcccgcgccc ggcggttaca tcgtcaatgc ggcaagctcg gtctcatcgt ggtcgattac
     4981 ctgcaactga tgacggcgcc atcaggccgc cagggcgaga accgcgcgac agagatctcc
     5041 gagatctcgc gctcgctaaa agccctggcg aaagaactcg atgtacccgt cattgcgctc
     5101 tcgcagctga atcgcggact ggagcagcgg ccggacaagc gtccggtgat gtctgacctg
     5161 cgcgaatccg gcgctatcga acaagatgcc gacctgatcc tgttcatcta ccgtgacgag
     5221 gtctacaacc cggaaagcac cgacaagggt acggctgaga tcatcatcgc caaacagcgt
     5281 aacggcccga tcggcaaggt ccggctgacc ttcatcggcg cccacacccg attcgagaat
     5341 ttcgtcaacg ccggcgcctc ttactgagcc gatgcgtccg atccaagcca ccatcgacct
     5401 gggcgccttg cgccataatc tgaacgttgt ccggcagcat gccccgaacg ccagggtgat
     5461 ggccgtgatc aaagccaatg gctatggcca tggcctgctg cacgtagcgc aaggtctgca
     5521 atctgccgac ggcttcgccg tactcaatat cgaagaggcg atcgccctgc gcgaagcagg
     5581 atatcggcaa cgcatcctgt tgctggaagg ctttttcacc gacgacgaga tcgcgcttgc
     5641 agcaagccat gacatcaccg tcgtgctgca tgcggattgg cagctcgacc ttctggcgcg
     5701 ccagccgcag ggcatccggc ttcctgtatt catcaagctc aataccggca tgaatcggct
     5761 gggcttcatg ccagccgcct tgaccgaagc gctggcgcgc attgacgcac tgccgatgat
     5821 cgacgacatc accctcatga cccatttcgc caccgccgat gagccgcgcg gtatcgccga
     5881 agcgcatgcc ctgttcgagc aggccacggc gggcctggct tacccacgat ccatggctaa
     5941 ttccgccact atattgcgtt atccgcaagc gcatgcggac tgggtgcgcc cgggcatcat
     6001 gctctacggc gcgagcccgg tcagtggcac gcctgccgcc cagttcggct tgcgtccggc
     6061 gatggcgctc actagcgaac tgatcgccgt gcagacgctg cagcctggcg atagcgtcgg
     6121 ttacggcatg cgctacaccg ctgagaaacc gacccgtatc ggtatcgtcg cctgcggcta
     6181 tgctgacggt tatccgcgac acgccggatt atgcgccgct ggcgaacgtc agccgggcgc
     6241 gccgatcgct gtcgccggga aactgacgca tacggttggg cgggtttcca tggatatgtt
     6301 attcgcggat atcaccgata tccctgaagc cgatatcggc agcccggtcg agctgtgggg
     6361 cgcgcaggtg ccggtcgatg ctgtggctga agccagcggc acggtcggct atgaattgct
     6421 gtgtgcgctg gcaccgcgtg taccagtcag ggtcatcgac tgatggcgaa gcaaaagacc
     6481 gtctatacct gtactgaatg tggtggccag gagcccaagt ggcaaggcca gtgcccgaat
     6541 tgcggacaat ggaacacgct ggtcgagacc gtggccgaac cggtgacggc caatcgttac
     6601 gccgcactgg cgcaacccac gggcttgcag aagctggcgg aggtcgaggc agcagaggtg
     6661 ccgcgccacc cgaccgcgat cagcgaattc gaccgcgtgc tcggcggcgg cctcgtcccg
     6721 ggtggcgtag tgctgatcgg tggtgatccg gggatcggca aatcgacctt gttgctgcag
     6781 acgctttgcc acgtcgggcg ccaacgtaaa gccttgtata tcagcggcga ggaatcggcc
     6841 cagcagatcg ccatgcgggc caaacgtctg gggctggacg ccagccccat cgatctcctg
     6901 gccgagatcc agctggagaa gatcctcgcg accttgcaga accaccggcc ggatgtcgcc
     6961 gtcatcgatt cgatccagac cgtatactcc gaagccctgc aatccgcgcc aggctcggtg
     7021 gcgcaggtgc gcgaatgctc ggcccagctc acgcgcgtcg ccaagcagct cggcatcacc
     7081 gtcatcctgg tcggccacgt gaccaaggag ggcgcactgg ccggaccacg cgtgctggag
     7141 catatcgtag acaccgtgct gtatttcgag ggtgacccca attccagttt ccgcctgatc
     7201 cgcgcattca agaatcgctt cggcgcggtg aacgagctcg gcgtgttcgc catgactgaa
     7261 aagggtctgc gtgaggtcag caacccctcg gccttgttcc tttcgcatca taccgaacag
     7321 gtcgccggtt cctgtatcac agtcacgcag gagggtacgc gccctttgct ggtcgaggtg
     7381 caggcactgg tcgacgaagc ccatgcgccg aatccgaagc ggttatgtgt gggccttgag
     7441 cagaatcgcc tcgccatgct actggcggtg ctgcatcggc atgccggcgt cgcctgctac
     7501 gaccaggacg tgttcgtcaa tgccgtcggc ggggtcagga tcagtgaacc ggcagtcgat
     7561 ctcgcggtat tgctggcgat cgtatcatca ttgaaaaaca aggcattgga tagcaaactt
     7621 atcgtatttg gtgaagttgg tctggcaggt gaggtgcggc cggtacagcg cggccaggag
     7681 cgtctgaaag aggcggccaa gctcggtttc acccatgcca tcgtgcccaa gagcaatgcg
     7741 cccaggcagc cgatcaaagg catcgagatc tttgcggtgg atcgcttgga gcaggctttg
     7801 cagaaagcca gggagatctg agcttgcaga cagcggcatg gcaaagcgct tcaccgacga
     7861 gaagcagcgt catcttcgat ctcgacggca cactcattga ttcttccgcc agtattctgc
     7921 gcagcttcga ggcggcattt gacaagatcg gcgtcatcgc gcgtcaggcc tttacctcag
     7981 aagtcatcgg tccgccgctg atcccgacac tgcagcggct cgcgggctcg gacgatccgg
     8041 cattgataca agccctggcg acagcgttca aggtcgagta cgacagccat gcctatcagg
     8101 ctgcgaccgt gttcgacggt atccccgaat tgctggccag cttggggcag cgtggcgtgc
     8161 agatgtatat cgctaccaac aagcgcatcc tgccgacgcg taagatcctc gcgcatctgg
     8221 gctggcagga gcattttgcc ggcatttacg gcttggatta ttttgagcca gcggcggctt
     8281 caaaaacgga gatggtgacg cggatcatcg aacgccatgc gctgacgcct gaactgaccc
     8341 tgttcgttgg tgaccggatc gaggatggga tagccgccga aggcaacggc ctcgatttcg
     8401 ccatggtcac ctggggttat gtcgatgata cccagggtga acgcagtgac aactggcagt
     8461 acttcgacga tccgcatgcg cttggcatct acatcacagg ctaagtcttg ccgaagtacg
     8521 gacggcatcg ctccggtttt atgcgccatg caggtggcat gcacacggct gacctggcgg
     8581 gtgacgctag agatgcgttg ccagcgtcag gtcgatcacc tcgcctgacg gcgtaacatt
     8641 atccggcccc atcaggtaaa ggtaggcggg catcaagcgc tctgcttccg gccaggcctg
     8701 gggaacttcg ccaggatgcg atttcaggcg ctgcggcgac tgtaccggtc cgggtacgac
     8761 gatgttcatc tgcaacttgt tcggtgtccc caattcctgc gcccaagtct gcgccatgtg
     8821 caccactgcg gccttactga cgccatgcgc cccccagaag gctttggcat gctgtccact
     8881 ggtcgatgac gtgaacagca cggtcgcctg tggcgcacgc cgaagcagcg gtaggcaggc
     8941 cttggtgagc gcgaacggcg cgatcagatt gaccttgagc atggtctcaa actgcgaggc
     9001 ctgctgcagc tcgagcgggc tgaggttatc gaaatgcgcg gcgttatgca ggatgccgtc
     9061 cagcctgcct aactgctggt agatgccttc cgccatggcc tgatagtcgg tttcctgcgc
     9121 gtccttcagg ttcaggggga agatcagggc ctcaggatag cctgctgccg cgatctcgtc
     9181 gtagacgcgt tccagcttcc ttacgtcgcg tccgagcagg atcaccgtcg cgccatgcct
     9241 ggcgaaagcc actgcggcgg cccggccgag gccctggcca gcgcctgtga ccaggatcac
     9301 gcgcgtatgg agtagatcgg gtggggcttg ataagcttgc atggccagtc gtttcagcta
     9361 aagaggcgtg attgtacctg catcgggcgt tcacacggtg ggcaggatgg cgaacccgac
     9421 tacttttgga acctcacatt cgcgtatatc aacatgaacg accttggcca gccctggccc
     9481 ctgataagtc cagtcgatca ctgcttgcac gcgggcgtca tcaccgctga ggactgcttc
     9541 gaccgtgccg tctcgccggt tgcgcaccca gccttcaagg ccagcggcag tcgctgacgc
     9601 ttgtagcgat tgccggaagc cgacgccttg cacgcgacca tggatgcgca gttgtctcgc
     9661 gatcatccgt gagccgtatc agatattcac ggtatgcatc gacggcacta cgatcagatg
     9721 cgtgttgccc ggcaataccc actccaggaa gaagccgaag atagcgatga
//
LOCUS       DATMTW010000202         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_219607, whole genome shotgun sequence.
ACCESSION   DATMTW010000202 DATMTW010000000
VERSION     DATMTW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219607"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VN084_03900"
     CDS             complement(<1..1206)
                     /locus_tag="VN084_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830444.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWT28630.1"
                     /translation="MMRQYLSIKAQYPDMLLFYRMGDFYELFHDDAEKAARLLGITLT
                     KRGASNGAPIRMAGVPYHAAEQYLAKLAKLGEAVAICEQVGDPKTSKGPVERQVSRIL
                     TPGTLTDSALLDDTRDSLLLAICPGDGLLGLARINLASGEFVLSEIAVGLLSQELERI
                     GPAELLLPEDFSHAALDAVRCPKKRMAPWQFDLDSATQSLTKQFDTYDLGGFGCQDMP
                     SAIAAAGALLDYVKHTQRTALPHLAGLSVEQTHDYLQMDAATRRNLEIDQTLRGEAAP
                     TLYALFDTTQTAMGARLLRHWLHHPLRDHATIMARQAAVAGSIASAAYVSVRGVLANI
                     GDIERITARVALKTARPRDLSGLRDSLTLLPTLQAAMHDIATPLWQALVADLQTPVAV
                     TELLQSAIRP"
     gene            complement(1296..1661)
                     /locus_tag="VN084_03905"
     CDS             complement(1296..1661)
                     /locus_tag="VN084_03905"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HWT28631.1"
                     /translation="MLAFEAIAEQRISEALARGEFERLPGAGQPLDLDDDRLVPADLR
                     MAYRILKNAGYVPPEVHTLNEIGALQRLVQSLADGEQREMALRKLRLLSMQLGESRGG
                     PLQLDAVYQDGLLKRLSQS"
     gene            complement(1667..2473)
                     /locus_tag="VN084_03910"
     CDS             complement(1667..2473)
                     /locus_tag="VN084_03910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWT28632.1"
                     /translation="MHPILTNAVKAARRAGTIITRASQDVGALKIRSKTYNDFVSEVD
                     HAAERAIIETLQDAYPDHGFLGEESGDTNPDAENIWIIDPLDGTTNFLHNFPQYCVSI
                     ALMQRGQLTQAVVYDPNRNDLFTATKGRGAFLNDKRIRVTNRIKLQDSLIGTGFPFRD
                     FSHLDTYMPMLKDMIKKASAIRRPGSAALDLAYVAAGYLDGFWEIGLSKWDIAAGALL
                     VQEAGGIVADFEGNESWFESGNIVAANPKVFAQMLQVLTPHLTPALKTPA"
     gene            2563..3309
                     /locus_tag="VN084_03915"
     CDS             2563..3309
                     /locus_tag="VN084_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWT28633.1"
                     /translation="MTSKHDPLDNLRIVLCQTSHPGNIGASARAMKTMGLRQLYLVSP
                     ESFPDAHATAMATGAANLLKDATVCSTLEEALAGCALVIGLSARKRQLSHELLAVRPA
                     AERALSVAQTQQVALVFGTEMSGLSNAELDLCQLLAMIPANPEFSSLNLAAAVQVVCY
                     ELRMAALASDTAAIASQPMPMATSDEIEGFYRHLEQTLVQIGFLNPDEPKKLMQRVRR
                     IYARAKLEKEEVNILRGILKLTQAPRPPAK"
     gene            3454..4206
                     /gene="cysE"
                     /locus_tag="VN084_03920"
     CDS             3454..4206
                     /gene="cysE"
                     /locus_tag="VN084_03920"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWT28634.1"
                     /translation="MFKHLKEDISVVFDRDPAARTRWEILTTYPGVHALIMHRLSHWL
                     WARRWYWIARFSSHIARWFTGIEIHPGATIGRRVFIDHGMGVVIGETAVIGDDCTLYH
                     GVTLGGTSWNKGKRHPTLERGVVIGAGAKVLGPIVIGAEAKIGSNAVVVKDVPAGATA
                     VGIPARLVDQEKSQQRDQMAEKIGFSAYAVSQDNKDPMTQAIHALLDHAAKQDQKLQE
                     VMQQLAKLGADVETDAEVGKAFDVNYLNKIVD"
     gene            4314..4787
                     /gene="iscR"
                     /locus_tag="VN084_03925"
     CDS             4314..4787
                     /gene="iscR"
                     /locus_tag="VN084_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HWT28635.1"
                     /translation="MRLTTKGRFAVTAMLDLAMHETDKPVTLAGISERQGISLSYLEQ
                     LFSRLRRNGLVKSVRGPGGGYKLAKQKQDISVSHIISAVDELIDATQCGGQENCRGER
                     RCMTHDLWASLNSKILEYLSGVSLSDLVASQSEGTQLVMESRPCTMQPKIASVAA"
     gene            4801..5952
                     /locus_tag="VN084_03930"
     CDS             4801..5952
                     /locus_tag="VN084_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWT28636.1"
                     /translation="MSNGAVYFDHNATTALDSRVLQAMLPYLSEQQGNATSRHSFGRA
                     ARHAIDLAREQVADAVAAHPSQVVFTAGGTEANNLAISGIARRYGPSQIIVSAIEHPC
                     VSRPAAALRNEGWQLQTLPVDAAGRTDQTSLETMLRTDTCVVSAMLVNNETGVIQDVA
                     AIAEQARRGGAYMHTDAVQALGKTHVDFSALNVHAMTLSSHKINGPQGAGALIVDKRV
                     DLQPVLYGGGQEKGLRSGTENVAAIVGFGAACALIKHELAQRAETARLLRDRLEQGLS
                     QLGAHIFGSGAARIANTSFFAFPDIEGETLVMALDRHGFAVASGSACSSDSTEPSQVL
                     LAMGVPHDLARGAIRVSFGVGNELQQVDDFLLALSHELQRLKQLTAIAV"
     gene            5963..>7110
                     /locus_tag="VN084_03935"
     CDS             5963..>7110
                     /locus_tag="VN084_03935"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWT28637.1"
                     /translation="MTVHTPIYLDYSATTPVDPRVAEKMIPYIAEHFGNPASRSHSFG
                     WTAEKAVENAREEVAKLVNGDPREIVWTSGATESNNLAIKGAANFYSGKGKHIITVLT
                     EHKAVIDPVRELERQGFEATFLEPEPNGLIDLEKFKAAIRPDTVLASVMLVNNEIGVI
                     QDIEAIGNLCRANNVIFHVDAAQATGKVAIDLEKLPVDLMSFSAHKTYGPKGIGALYV
                     RRKPRIRLEAQIHGGGQERGFRSDTLATHQIVGMGEAFRLAREEMDLESARIRRLRDK
                     LLAGLEEIEEVYVNGDLKQRVPHNLNISFNYVEGESLIMAVKDIAVSSGSACTSASLE
                     PSYVLRALGRSDELAHSSIRFSIGRFTTEEDIDYTVDLMKSKIGKLREL"
BASE COUNT         1447 a         2210 c         2165 g         1288 t
ORIGIN      
        1 ggggcggatc gcggattgca gcaactcggt cacagccacc ggggtctgca agtccgcgac
       61 gagcgcctgc caaagtggcg tcgcgatatc gtgcatggcc gcctgcaatg tgggcagcag
      121 cgtcaggctg tcacgcaagc cgctgagatc gcgtggacgc gccgtcttca aggcgacgcg
      181 tgcggtgatg cgttcaatgt cgccgatatt ggcgagcacg ccacgcacac tgacataggc
      241 ggcgctggcg atgctacctg cgactgccgc ctggcgggcc atgatggtcg cgtggtcgcg
      301 taacggatga tgcagccaat ggcgcaagag tcgggcgccc atggccgtct gcgtggtatc
      361 gaacagggca tagagtgtgg gtgcggcctc accgcgcagc gtctggtcga tctccaggtt
      421 gcggcgcgtg gcggcgtcca tctggagata atcatgcgtt tgctcgacgc tgagcccagc
      481 caggtgaggc aaggcggtgc gctgggtatg cttgacgtaa tcgagcaagg caccagcggc
      541 ggcgatggcc gaaggcatat cctgacagcc gaaaccgccg aggtcatagg tatcgaactg
      601 cttggtcagt gattgggtcg cggaatcaag atcgaactgc cagggcgcca tgcgcttctt
      661 ggggcatcgg accgcatcga gagccgcatg cgaaaagtcc tcgggcaata gcagctcggc
      721 tggcccgata cgttcgagtt cctgcgacaa taacccgacc gcgatctcgc tgagcacgaa
      781 ctcacccgac gccaggttga tgcgcgcgag tcccagcagg ccatcgccag ggcaaatggc
      841 gagcagtagg ctgtcgcgcg tatcgtccag cagagcgcta tcggtcagcg tacccggcgt
      901 caggatgcga ctgacctggc gttcgaccgg gcctttactg gtcttggggt cgccgacctg
      961 ctcgcagatg gccacggctt cgccgagctt ggccagtttc gccaggtatt gctctgccgc
     1021 gtgatatggc acgccagcca ttcggatcgg cgcaccattc gatgcccccc gcttggttag
     1081 cgtgatgccg agcagccgcg ccgccttctc ggcatcgtcg tggaacaatt cgtagaaatc
     1141 gcccatgcgg tagaacagca gcatatccgg gtattgcgcc ttgatgctga ggtattgccg
     1201 catcatcggc gtatgctgcg ccaagctgtc tggctgcgcc gtctcgctgg ccgtgtccgc
     1261 aattactggc ggctcttgca tcgcgggtgc ttcactcatg attgcgaaag gcgcttcagc
     1321 aagccgtctt gatagacggc atccagttgc agagggccgc cacgtgactc gcccagctgc
     1381 atgctgagca agcgtaactt gcgcagtgcc atttcgcgct gctcaccgtc cgccagcgat
     1441 tgcaccagcc gctgcaacgc accgatctcg ttcagggtat gcacctcggg cggcacataa
     1501 cccgcgttct tgaggatacg gtaggccatg cgcagatctg ccggcaccaa ccggtcatcg
     1561 tcaagatcga gtggctgacc ggcacccggc agtcgttcga attcgccgcg cgccagggcc
     1621 tcgctgatgc gttgttcggc gatggcttcg aacgcgagca tgtcgatcag gcgggagtct
     1681 tgagtgcagg cgtcaggtgc ggcgtcagca cctgcagcat ctgcgcgaac accttggggt
     1741 tggcggcgac gatattgccg ctctcgaacc aggattcgtt gccttcgaaa tcagcgacga
     1801 tcccgcccgc ttcctgtacc agcaaggcgc cggcagcgat gtcccatttc gacaggccga
     1861 tctcccagaa accatccaga taaccggcgg cgacatacgc gaggtcgagc gcggccgagc
     1921 ccggacgacg gatagcggag gccttcttga tcatgtcctt cagcatcggc atgtaggtat
     1981 cgagatggct gaaatcgcgg aaggggaagc ctgtgccgat cagcgaatcc tgtagcttga
     2041 tgcgattggt gacgcggatg cgcttatcgt tgaggaaagc gccacgcccc ttggtcgcgg
     2101 tgaaaagatc gttgcggttc gggtcgtaga ccacggcctg ggtcagctgg ccacgctgca
     2161 tgagcgcgat ggagacgcag tattgcggaa aattgtgcag aaagttggtg gtcccatcca
     2221 gcggatcgat gatccagata ttctcggcat cggggttcgt atcgccactc tcctcaccga
     2281 ggaatccatg gtcaggatac gcgtcctgca aggtctcgat gatggcgcgc tcggcggcat
     2341 gatcgacttc actgacgaaa tcgttgtagg tcttgctgcg gatcttcagt gcgccgacgt
     2401 cctgcgaagc gcgggtgatg atggtaccgg cacgacgtgc cgccttcacc gcattggtca
     2461 aaatgggatg catagggtac tttctgcgaa tgccgcaccg gtgcaagcac aggcgaagcg
     2521 gtattaaaga gctgttaaaa tggcatttta atgtgtattg ccatgacttc caaacacgat
     2581 ccactcgaca acctgcgcat cgtcctctgc cagaccagcc atccgggtaa tatcggcgct
     2641 tcggcacgtg ccatgaagac catggggcta cgccagctct atctggtctc gcccgaatcc
     2701 tttcccgatg cgcatgccac ggccatggcg accggcgctg ccaatctgct taaggatgcc
     2761 acggtatgca gcacgctgga agaagcgctc gccggctgcg cgctggtgat cggactcagc
     2821 gcgcgcaaac ggcagctgtc gcacgagttg ctggcagtac ggccagcggc cgagcgcgcc
     2881 ctgtccgtgg cccagaccca gcaggtggcg ctggtgttcg gcaccgagat gagcggtctg
     2941 tccaatgccg agctcgatct ttgccagctg ctggcgatga tcccggcgaa tccggaattc
     3001 tcctcgctca acctggccgc agcggtccag gtcgtctgtt atgagctgcg catggccgcg
     3061 ctggccagcg acaccgcggc gatcgccagc caacctatgc cgatggcgac gagtgacgag
     3121 atcgaaggct tctatcgaca cctggagcag acgctcgtgc agatcggctt cctgaatcct
     3181 gacgaaccca agaagctcat gcagcgcgtg cgccgcatct atgcgcgggc caagctggag
     3241 aaggaagagg tcaacatcct gcgtggcatc ctcaaactca cccaggcgcc ccgaccaccg
     3301 gcgaaatagc cgggaaaacg gcaagggcca aataccccta cgcaaaatgt cgttgcatgc
     3361 cccccgtaat catcgagtaa aatagtcaac tattcacaag attaaaggat gagtcggtcg
     3421 cccaggcgca ccggctcaag atcaggcgca cacatgttca agcatctgaa agaggatatt
     3481 tcggtcgtat tcgatcgcga cccggcggcc cgcacacgct gggagatcct gacgacctat
     3541 cccggcgtgc atgccctcat catgcatcga ctttcgcatt ggctctgggc caggcgctgg
     3601 tactggatcg cccgcttcag ctcgcatatc gcccgctggt tcaccggcat cgagatccac
     3661 ccgggcgcca ccatcggccg tcgcgtcttc atcgaccatg gcatgggcgt ggtcatcggc
     3721 gagaccgccg tcatcggtga cgactgcacg ctctatcatg gcgtcacgct aggcggtacg
     3781 tcgtggaaca agggcaagcg ccacccgacg ctggaacgcg gcgtcgtcat cggcgctggc
     3841 gccaaggtgc tgggcccgat cgtcatcggc gccgaagcca agatcggctc taacgctgtc
     3901 gtcgtcaagg atgtgccggc cggcgccact gccgtgggca ttccggcccg tctcgtcgat
     3961 caggagaaat cccagcaacg cgaccagatg gccgagaaga tcggtttttc ggcctatgcc
     4021 gtcagccagg acaacaagga cccgatgacc caggctatcc atgccctgct cgaccacgcc
     4081 gccaagcagg atcaaaaact gcaggaggtc atgcagcagc tcgccaagct cggcgccgat
     4141 gtcgaaaccg acgccgaagt cggcaaggca ttcgatgtga actatctcaa taaaatagtt
     4201 gactaaactg ttagggtatt ataacattcc gctttggttt taagctggaa caccgcgccg
     4261 atattgaatc gggcaaatca tgcggaaccc cggacacttt aggaaacgat gacatgcgat
     4321 taaccaccaa aggccgtttt gccgtgacag cgatgctcga tctcgccatg cacgagaccg
     4381 acaaaccggt cacgctggcc ggcatcagcg agcgccaggg catctcgctg tcctatctcg
     4441 aacaattgtt cagtcgcctg cgccgtaacg ggctcgtcaa gagtgtgcgc ggccccggcg
     4501 gcggctacaa gttggccaag caaaagcagg acatctcggt ctcgcacatc atctccgccg
     4561 tcgacgaact catcgacgcc acccagtgcg gtggacagga gaactgccgc ggtgagcgcc
     4621 gctgcatgac acacgatctc tgggccagcc tcaacagcaa gatcctcgaa tacctttctg
     4681 gcgtatccct gtcggacctg gtcgcatcgc aatccgaggg aacacaactc gtgatggaga
     4741 gccgcccctg cacgatgcaa cccaagatcg catcggtagc ggcctgagga tacgtcagcc
     4801 atgagcaacg gcgccgtcta tttcgaccat aacgcgacca ccgcccttga cagccgcgtg
     4861 ctgcaggcga tgctgcctta tctgtcggaa cagcagggca acgccaccag ccgccacagt
     4921 ttcggccggg ccgcgcgcca tgccatcgac ctcgcgcgcg aacaggtggc cgatgcggtc
     4981 gccgcgcacc cgtcacaggt ggtcttcact gccggcggca ccgaagccaa taacctggcg
     5041 atcagcggca tcgcgcgccg gtatggtccg agccagatca ttgtcagcgc catcgaacac
     5101 ccttgtgtct cgcgaccggc cgccgccctg cgcaacgaag gctggcaact gcagaccctg
     5161 ccggtcgatg ccgcgggcag gacagatcaa acatcgcttg agacgatgct gcgcaccgac
     5221 acctgcgtgg tgtcggcgat gctggtcaat aacgagaccg gtgtgatcca ggatgtcgcg
     5281 gccatcgccg aacaggctcg ccgcggcgga gcctacatgc ataccgacgc ggtgcaggcg
     5341 ctgggcaaga cccatgtgga tttcagtgcg ctcaacgtgc atgcgatgac actgtcgtcg
     5401 cacaagatca acggaccgca aggcgcgggc gccctgatcg tcgacaagcg cgtcgatctg
     5461 cagccagttc tatatggcgg cggacaggag aaagggctgc gcagcggcac cgagaacgtg
     5521 gcggcgatcg tcggattcgg tgcggcttgc gcgctcatca agcacgagtt ggcacagcgc
     5581 gccgagacag cgcgcctgtt gcgcgaccgc ctggagcaag gtttaagtca gctcggcgcc
     5641 catatcttcg gtagcggcgc cgcgcgtatc gccaatacca gcttcttcgc ctttcccgac
     5701 atcgaaggcg agaccttggt gatggcgctc gatcggcatg gatttgcagt cgccagcggc
     5761 tcggcctgct ccagcgacag cacagagccc agccaggtgt tgctggccat gggggtcccc
     5821 cacgatctgg cacgcggcgc catccgcgtg agctttggcg taggcaacga actgcagcag
     5881 gtggacgatt tcctgctggc actgtcacac gaattacaac gattgaagca attaaccgcg
     5941 atcgcggtat aaggaagcga acatgactgt acatacacct atctatctgg attactctgc
     6001 gacgacgccg gtcgacccgc gtgttgcaga gaagatgatc ccttacatcg ccgaacattt
     6061 cggcaacccg gcttcgcgca gccacagttt cggctggacg gcggaaaagg ccgtggagaa
     6121 cgcccgcgaa gaagtcgcca aattggtcaa cggcgacccg cgcgagatcg tctggacttc
     6181 tggcgccacc gaatccaaca atctggcgat caagggtgca gccaatttct atagcggcaa
     6241 gggcaagcac atcatcacgg tgctcaccga gcacaaggcc gtcatcgacc cggtgcgcga
     6301 actcgagcgc cagggcttcg aagcgacgtt cctggaaccg gaacccaacg gcctcatcga
     6361 cctggagaag ttcaaggctg cgatccggcc ggatacggtg ctggcttcgg tcatgctggt
     6421 caacaacgag atcggggtga tccaggacat cgaagccatc ggcaacctgt gccgcgccaa
     6481 caatgtgatc ttccatgtcg acgcagccca agccactggc aaggtcgcga tcgacctgga
     6541 aaaactgccg gtcgacctca tgagcttctc ggcgcacaag acctacggcc caaagggcat
     6601 cggcgcgcta tatgtccgcc gcaagccacg tatccgcctc gaagcgcaga tccacggggg
     6661 tggccaggaa cgcggtttcc gctccgacac gctggccacg caccagatcg tcggcatggg
     6721 cgaggcgttc cgcctggcac gcgaagagat ggacctggaa agtgcccgca tccgtcgcct
     6781 gcgcgacaag ctgctggccg gcctggaaga gatcgaagag gtctatgtca acggcgacct
     6841 caagcagcgc gtgccgcata acctcaacat cagcttcaac tacgtcgagg gcgaatcgct
     6901 gatcatggcc gtgaaggata tcgccgtctc ttccggctcg gcttgtactt ccgccagcct
     6961 ggagccctcc tatgtgctgc gtgcgctggg ccgctccgac gaactggcgc acagctcgat
     7021 ccgtttctcc atcggccgtt tcaccaccga ggaagacatc gattacaccg tcgacctcat
     7081 gaagagcaag atcggcaagt tgcgcgaact
//
LOCUS       DATMTW010000203         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_285880, whole genome shotgun sequence.
ACCESSION   DATMTW010000203 DATMTW010000000
VERSION     DATMTW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285880"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..866
                     /locus_tag="VN084_03940"
     CDS             141..866
                     /locus_tag="VN084_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28638.1"
                     /translation="MQRDHFTLTHPAPLRLNDEEREALLLDINRHFVEDGLHFCQSET
                     GDWYLRMDETPQLQTAMLGEALGHDVREFQPQGADAARWKRIQNELQMLLHEHPVNHA
                     REARGLPAVNSLWLWGQGRLTPYTGNSPFTAVVAEDPFVRGLAAHTGVTRYPLAALDP
                     LTALDGDATLDAMLGADALIVMDAHDAACAARVNAMRDALRHRRLRRLKLSIVLRDRL
                     LVTELGPLDAWKLWRRPRSLTAY"
     gene            884..2593
                     /gene="recJ"
                     /locus_tag="VN084_03945"
     CDS             884..2593
                     /gene="recJ"
                     /locus_tag="VN084_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWT28639.1"
                     /translation="MVTIVQRPYDPSAADLLAASGLPGPLARVLAARGIHAPAQLDAS
                     LAGLIPPERLTNSVKMAQLLADAIAQGKRLLVIGDYDADGATATAVAVRGLRGMGATV
                     DFLVPNRFEYGYGLTPEIVALAATRQPDIIITVDNGIASVDGVRAANTLGIPVLVTDH
                     HLPGDETPEAACILNPNQRGCDFPSKHLAGVGVMFYALLALRAELRIRGAFATRQEPN
                     LTELLDLVALGTVADLVRLDDNNRILVEQGLRRIRAGKACPAIPALMRLAGRDPSQAG
                     AQDLGFSVGPRLNAAGRLDDMSLGIECLLADDPAKAQQMAQQLHELNLERRNIEADMH
                     DAALASLEGIEVADRYSLSLYDADWHQGVIGILASRIKERYHRPVIAFADAGDGILKG
                     SGRSISGLHLRDALDLVSKRDPLLLLKFGGHAMAAGLTIRACDLDAFVAAFEGVAREL
                     LRPADLESLIETDGALDGQDIDWSTACAIERQVWGQGFPQPLFSDRFRVLDQRVVGER
                     HLKLVLEKQGRRFDAIFFRQQEYLPAEVEAVYQLQTNQYNGAQSVQLSLRHWQPVTST
                     QPG"
     gene            2604..3359
                     /gene="tpiA"
                     /locus_tag="VN084_03950"
     CDS             2604..3359
                     /gene="tpiA"
                     /locus_tag="VN084_03950"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWT28640.1"
                     /translation="MRRKLVVANWKMNGSLARNQALLETARTGLAPLKGADFAICVPY
                     PYLFQAQAILQGSNVSWGAQNLAKHEEGAYTGEVSAAMLKDFGASYVIIGHSERSSAL
                     CESDVNIAAKFALAKAFDITPILCIGETLGEREAGVMEYSIANQLDAVLSVSDGALLE
                     DAVVAYEPVWAIGTGKSATPDQAQAAHAFIRDRIKALNPAAANSVRIIYGGSVKPSNA
                     SQLLAMPDIDGGLVGKCSLNAGDFEEICRAACG"
BASE COUNT          589 a         1057 c         1071 g          644 t
ORIGIN      
        1 tgacgccgcc tgcgctgccc gggtgaacgc gatgcgcgac gctttacgcc atcggcgcct
       61 gcgccgcgct ttcgtggctg ggcgccggtc atgaccccgg ggcgacgtgc tggatgcatg
      121 cagacccggt ccatctcgca ttgcagcgcg accatttcac gctgacccat cccgcaccgc
      181 tcaggctcaa cgacgaagag cgcgaggcct tgctgctcga catcaatcgc cacttcgtcg
      241 aggatgggct gcatttctgc caaagcgaga cgggggactg gtacctgcgc atggacgaaa
      301 cgccgcaatt gcagaccgcg atgctgggcg aagcgctagg ccatgacgta cgtgagttcc
      361 agccgcaagg ggcggatgct gcgcgctgga agcgcatcca gaatgaactg cagatgctct
      421 tgcatgagca cccggtgaac cacgcgcgcg aagcccgtgg cctgcccgcc gtcaacagcc
      481 tctggctctg gggccaaggt cgattgacgc cttacaccgg gaatagccca ttcacggccg
      541 tggttgccga agaccccttc gtccgcggtt tggccgcgca taccggggtc acgcgttatc
      601 cattggccgc gcttgatcca ttgaccgcac ttgacggcga tgcgacgctc gacgccatgc
      661 tgggcgccga cgcgctgatc gtcatggacg cccatgacgc cgcctgcgct gcccgggtga
      721 acgcgatgcg cgacgcttta cgccatcggc gcctgcgccg cctgaagctc agtatcgtcc
      781 tgcgcgacag gctgctggtc acggagctcg ggccgctcga cgcctggaag ctctggcgtc
      841 ggccccgatc cttaaccgct tactgaaggc cacgacttat cttatggtta cgatcgtcca
      901 acgtccctac gatccctcgg ctgccgacct gttggcggcc agcggcctgc ccggaccact
      961 ggcgcgtgta ctcgccgcgc gcggcatcca tgcgccagcg caactggacg ccagcctcgc
     1021 gggcctgatc ccgccggaac ggctgaccaa cagtgtcaag atggcgcaat tgctggcgga
     1081 tgccatcgcc cagggcaagc ggctcctggt catcggcgac tatgatgccg acggcgccac
     1141 ggcgacggca gtggctgtgc gcggcttgcg cggcatgggc gcgacggtgg atttcctcgt
     1201 gcccaaccgg ttcgaatatg gctatggtct gacgcccgag atcgtcgcgc tggctgcgac
     1261 gcggcaaccg gatatcatca tcacggtgga taacggtatc gccagcgtgg atggcgtgcg
     1321 tgccgccaat accctcggca tcccggtgtt ggtgaccgat caccacctgc cgggcgacga
     1381 gacgccggag gcggcttgca tcctcaaccc gaaccagcgt ggctgcgatt ttcccagcaa
     1441 gcacctggct ggcgtcgggg tcatgttcta tgcgttgctg gccttgcgcg cggagctgcg
     1501 cattcgcggc gctttcgcca cgaggcagga acccaacctc accgagctgc tcgaccttgt
     1561 cgcgctcggc acggtggctg atcttgtcag gctcgacgac aataaccgca tcctggtcga
     1621 gcagggcttg cggcgcatcc gtgccggcaa ggcgtgtccg gcaatcccgg ccctcatgcg
     1681 gctggccgga cgcgacccca gccaggctgg cgcacaggac ctgggctttt ccgttgggcc
     1741 acgcctcaac gctgctgggc ggcttgatga catgtcgctg ggtatcgaat gcctgcttgc
     1801 agacgatccg gcgaaggcgc aacagatggc tcagcagttg cacgaattga acctggagcg
     1861 gcgcaacatc gaagctgata tgcatgatgc ggccctggct tcgctcgagg gtatcgaagt
     1921 ggcggatcgc tacagcctca gtctgtatga cgccgactgg catcagggcg tgatcggtat
     1981 cctcgcttcg cgcatcaagg agcgttacca ccgtccggtg atcgctttcg ccgatgccgg
     2041 cgacggcatc ctcaaaggct cgggacggtc gatcagcggc ctgcatctgc gtgacgcgct
     2101 cgatctcgtt tcgaagcgtg atcctctgct gctgctcaag ttcggcggcc atgccatggc
     2161 ggctgggctc accatccgcg cgtgcgatct cgatgctttc gtagccgcct tcgagggcgt
     2221 cgcacgtgaa ttgctgcgtc cggccgatct tgagtcgctg atcgagaccg atggcgccct
     2281 cgacgggcag gatatcgact ggtcgacagc ctgcgccatc gagcgccagg tatgggggca
     2341 aggttttccg cagccactgt tcagcgaccg gttccgcgta ctggaccagc gtgtcgtcgg
     2401 cgagcgtcat ctcaagcttg tgcttgaaaa gcagggtcgg cgtttcgacg ccatcttctt
     2461 ccggcagcag gaatatctgc ctgccgaggt ggaggcggtc tatcaactgc aaactaatca
     2521 gtataatggc gcgcaatcgg tgcagctctc gttgcggcac tggcagcctg tcacctcaac
     2581 tcaacccgga tagagccaga tcgatgcgtc gcaagttggt agtcgcaaat tggaagatga
     2641 acggtagcct ggccaggaac caggccctgc tggaaacggc aaggaccggt ctggcgccgc
     2701 tcaagggcgc ggattttgcc atctgcgtgc cttatcctta cctgtttcag gcgcaagcca
     2761 tcctgcaggg cagcaatgtg agctggggcg cccagaatct ggccaagcac gaagaaggcg
     2821 cttacaccgg cgaggtcagc gcggccatgc tcaaggattt tggcgccagc tacgtcatca
     2881 tcggccattc cgaacgcagc tccgcgctct gtgagagcga tgtcaatatc gctgccaagt
     2941 tcgcgctggc caaggctttc gacatcacgc ccattctctg tatcggagag acgctgggcg
     3001 agcgggaggc cggggtgatg gaatactcga tcgcgaacca gttggatgcc gtgttgtcag
     3061 tttcagacgg cgcgctgctg gaggatgctg tcgtcgccta cgagccggtg tgggccatcg
     3121 gtaccggcaa gtcggcgacg ccggatcagg cgcaggccgc gcacgccttt atccgggacc
     3181 gtatcaaggc ccttaatccg gctgccgcga acagtgtgag aatcatctat gggggtagcg
     3241 taaaaccctc aaatgcgtca caattactag ctatgccgga tatcgacggt ggcttggtcg
     3301 gtaaatgttc tttgaacgcc ggcgattttg aagagatttg tcgcgctgct tgcggctaag
     3361 c
//
LOCUS       DATMTW010000204         1607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_186312, whole genome shotgun sequence.
ACCESSION   DATMTW010000204 DATMTW010000000
VERSION     DATMTW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186312"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1607
                     /locus_tag="VN084_03955"
     CDS             <1..>1607
                     /locus_tag="VN084_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWT28641.1"
                     /translation="ANAAMASDSIPELTRLTGKVDFTETGVRAQNVTAVAYGGPVAFN
                     VSTGSDGTTQIAARGRVTDTGLQQALGPGLADRLDGMTDWYADIAVHKTGTDLLLRSN
                     LLGMASTLPPPLAKAATDRMVLRVEKKQTATDQDLVSLHLDNLLNAKLARVDHGGVMQ
                     IERGEIGLNAPAELPAQTGLDIKGSLDSLDIDEWRNLLDQSTTASNASGGDSLPINKI
                     DVAINQLDAFDRRINNLKLNAKAISEGWQMAIQSKEMTGDAQWSRVGNGKITARLKYL
                     TTPAATPGTAELRTQGNFKQQAQRYPALDVIAENFEMGPKKLGRLELLASEQDDDWSI
                     EKLKLITPESTLSAEGEWHNWKRNPNTRINLSWDIKDIGKTLERYGYPNTFKGGDADL
                     SGQLKWPGSPHEFDIPGLSGALQLDARKGQILKLQPGVGRLFGVLSLQSLPRRLTLDF
                     RDVFSSGFTFDKISASVRIDKGIMRSDDFRMEGPVALVEMKGETDLKKETQHLFIKVT
                     PYISDSLSIAALAGGPLAGAAAFVAQK"
BASE COUNT          363 a          518 c          480 g          246 t
ORIGIN      
        1 ggccaacgcg gcgatggcaa gcgacagcat ccccgaactc acgcgcctga caggcaaggt
       61 cgatttcact gaaaccggcg tgcgcgcgca gaatgtcacc gctgtcgcgt atggcggtcc
      121 ggtcgccttc aacgtcagca ccggcagcga tggtacgacc cagatcgccg cccgtggccg
      181 cgtcaccgat accggactgc aacaggcact cgggccaggg ctggccgacc gcctcgacgg
      241 catgaccgac tggtatgccg atatcgccgt gcacaagacc ggtaccgacc tcttgttgcg
      301 ctccaacctg ctcggcatgg cttccacgct accgcccccg ctggccaagg ctgcgaccga
      361 tcgcatggtg ctacgtgtag agaaaaaaca gacggcgacg gatcaagacc tcgtcagcct
      421 gcatctcgac aacctgctca acgcaaaact ggcgcgcgtg gatcatggtg gcgtcatgca
      481 gattgaacgt ggcgagatcg gcctcaacgc gccggccgag ctgcctgcgc aaacagggct
      541 cgatatcaag ggttcgctcg atagcctgga catcgacgaa tggcgcaacc tgcttgacca
      601 atcgacaact gccagcaacg cgtcaggcgg cgatagcctg ccgatcaaca agatcgatgt
      661 cgccatcaac cagctggatg ccttcgaccg tcgcatcaac aatctcaagc tcaacgccaa
      721 agccatcagc gaaggctggc agatggcgat ccagagcaag gagatgacag gcgacgcgca
      781 atggagccgc gtcggcaacg gcaagatcac cgcgcgcctc aagtacctga cgacgcctgc
      841 cgcgacgccg ggtacggcag aactgcgcac gcagggcaac ttcaagcagc aggcacaacg
      901 ctatccggct ttggacgtga tcgccgagaa tttcgaaatg ggcccgaaga agctgggccg
      961 gctggaactg ctggcctcgg agcaggacga cgactggagc atcgaaaagc tgaagctcat
     1021 cacgccggaa agcacgctct ccgccgaggg cgaatggcat aactggaaac gcaaccccaa
     1081 cacccgcatc aacctcagct gggatatcaa ggatatcggc aagacgctgg aacgctacgg
     1141 ctaccccaac accttcaagg gtggcgatgc cgatctcagc ggccagctca agtggcctgg
     1201 tagcccgcat gagttcgata tccccggctt gagcggcgct ttgcagctcg acgcacgcaa
     1261 gggccagatc ctcaaactcc agccgggcgt cggccgcctg ttcggcgtac tgagtctgca
     1321 aagcctgccg cgacggctga cgctggattt ccgcgacgtg ttcagtagcg gcttcacctt
     1381 cgacaagatc agcgccagcg tacgcatcga caagggcatc atgcgtagcg acgatttccg
     1441 catggaaggc ccggtcgcgc tggtcgagat gaagggcgag accgacctca agaaagagac
     1501 ccagcatctg ttcatcaagg tcacccctta tatcagcgac agcctgtcga tcgccgcgct
     1561 ggcaggcggg ccgctggccg gtgcggcagc ctttgtggcg cagaaac
//
LOCUS       DATMTW010000205         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_86085, whole genome shotgun sequence.
ACCESSION   DATMTW010000205 DATMTW010000000
VERSION     DATMTW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86085"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VN084_03960"
     CDS             <1..506
                     /locus_tag="VN084_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWT28642.1"
                     /translation="SGHYLRECLDIIHGLENVDLYLSKAAAEILQQYGYQHQVGRVYQ
                     DKTASSVPVELFYEGKYHTLVIAPATSNTVAKMVAGISDNLVTNLYAQAGKTRVPSIV
                     FACDTEPELESEAPRDNLVKVYPRRIDLENMERLKTFEETTVAGDMRQLDTAIRSRLS
                     CLKTSSS"
     gene            482..1882
                     /locus_tag="VN084_03965"
     CDS             482..1882
                     /locus_tag="VN084_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HWT28643.1"
                     /translation="MSENLLFLTGRLAEKSLQKVLAGMQPTDFKYRIAQIGVSVAALM
                     TPELILRRLPDAGDADRIVLPGLCRGDLNLLEAKYGIPAERGPEDLKDLPQYFGQGGK
                     PPDLSRHDVKIFAEIVEAPDLSVEGILRRARRYREQGADVIDLGCLPNTPFPHLAEAI
                     QALKAEGYQVSVDSLSSEDLLLAGRSGADYLLSLTEKTLWIADEVAAVPILIPAKPGN
                     LASLYRAIEGMQKKQKPFIADAILDPIPFGLTQSIVRYQRLRKRYPDIEIMMGVGNLT
                     ELTDADTTGINAILFGVIAELGLNAVLATAVSPHAANAVAEADVARRVMYAAREDRRL
                     PRDYSDGLLGLHDRRPFTYSAEEIAELAAQIKDPSFRILVSEAGVHIFNRDGLFHDTD
                     PFRLYPHLKVDDDASHAFYLGVELARAQIAWQLKKRYQQDEELQWGCSATSPPKDGRV
                     AHRNASLKEKREKKKS"
     gene            1882..2457
                     /locus_tag="VN084_03970"
     CDS             1882..2457
                     /locus_tag="VN084_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HWT28644.1"
                     /translation="MILEAIITSVDASQQAHIAPFGVREHGELVVIAPFRPSISLDNL
                     LSSRSAVINLTDDVRIFAGALTGRRTWPVRPAERVSGYVLEAALAHRELELVEVKDDV
                     LRPELYFRVVHEANHRPFRGFNRAQAAVIELSVLVSRLHMLPIDKIDNELAYLSIAME
                     KTAGEREMEAWRWLIEAVENHRAAIRGDNQA"
     gene            2454..>2982
                     /locus_tag="VN084_03975"
     CDS             2454..>2982
                     /locus_tag="VN084_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HWT28645.1"
                     /translation="MSAPGQTSRLLVSVSSLAEAEMVMQAGVDILDLKNPHAGALGAL
                     ELSDIRSIVNATDGRKLVSATIGDVPMQPACIVDRVDDTLSTGVDIVKIGFTDAAERD
                     ACIVALGDIARRGARLVAVLFADAGYDLSVLSMLKTAGFYGVMIDTAVKDGRHLMDHM
                     TLAELRDFVQTARELG"
BASE COUNT          627 a          825 c          900 g          630 t
ORIGIN      
        1 gttccgggca ttatctgcgc gaatgtctcg acatcatcca tgggctcgag aacgttgatc
       61 tgtatctgag caaggcggcg gcagagatct tgcagcagta cggctaccag catcaggtcg
      121 ggcgcgtgta tcaggacaag acagcaagca gcgtaccggt cgaattgttc tatgagggga
      181 agtatcacac cttggtcatc gctccggcca cgtccaacac ggtggcgaag atggtggccg
      241 gcatctccga caatctggtt accaacctct acgcgcaggc aggcaagacc cgcgtgccta
      301 gcatcgtgtt cgcctgcgat accgagcctg agctggaatc tgaagcgccg cgtgacaatc
      361 tcgtcaaagt ctatccgcgt cgcatcgatc ttgaaaacat ggagcgactc aaaacatttg
      421 aggagacgac cgtggctggc gatatgcgac agctcgatac ggcgatccgg tcgcgactct
      481 catgtctgaa aacctcctct tcctgaccgg aaggctcgca gaaaagagcc tgcagaaggt
      541 cttggctggc atgcagccga cagatttcaa gtatcgtatc gcccagatcg gcgtctctgt
      601 cgccgcactg atgacgcccg agttgatcct gcgccgtttg cctgacgcgg gcgacgccga
      661 caggatcgtc ttgcctggcc tctgtagggg tgacctgaac ctgctggaag ccaaatacgg
      721 catcccggcc gagcgtggcc ccgaggatct gaaagatctg ccccagtatt ttgggcaagg
      781 cggcaagcca cctgatctat ccaggcacga tgtgaagatc ttcgcagaga tcgtggaggc
      841 tcccgacctc agtgtcgagg gtatcttgcg gcgcgcgcgc cgttatcggg agcagggcgc
      901 ggatgtcatc gaccttggct gcctgccgaa tacgccgttc ccgcatcttg cggaggctat
      961 tcaagcactc aaggcggaag gatatcaggt cagtgtggat tcgctcagta gcgaagatct
     1021 cttgcttgca ggacgctcgg gtgccgatta ccttctgagc ctgaccgaga agacgctctg
     1081 gatcgccgat gaagtcgcgg cggtgccgat ccttatccct gccaagccag gcaatctcgc
     1141 ctcgctttat cgtgccatcg agggcatgca gaagaaacag aaaccattta ttgcagatgc
     1201 gatcctggat ccgatccctt tcggtctgac gcagtcgatc gtgcgctatc agcgtctacg
     1261 caagcgctat cccgatatcg agatcatgat gggcgtcggc aatctcaccg aacttaccga
     1321 tgcggatacc accgggatca acgccatact cttcggtgtg atcgccgagc ttgggttgaa
     1381 cgccgtcctg gcgactgccg tcagtccgca tgctgccaat gccgttgccg aagcggatgt
     1441 cgcaagacgc gttatgtatg ccgcacgcga ggatcgacgt cttccgagag actacagcga
     1501 tggcctgctg ggtttgcatg accgccggcc atttacttac agcgccgagg agatcgccga
     1561 gctggcagcc cagatcaagg atcccagttt ccgcatcctg gtcagcgaag caggtgtgca
     1621 tatcttcaat cgggacggtt tgttccacga tacggatcct ttcaggctct atccgcatct
     1681 caaggtcgat gacgatgcct cgcacgcctt ttatcttgga gtcgaactcg cgcgcgcgca
     1741 gatcgcctgg cagttgaaaa agcgttacca gcaggatgaa gagctacagt ggggctgcag
     1801 cgcgacatcg ccccccaagg atgggcgcgt ggcgcatcgc aacgcatcgc ttaaggaaaa
     1861 gcgggagaaa aagaagtcat gatgattctt gaggccatca tcacgagtgt cgatgccagc
     1921 cagcaagcgc atatcgcgcc attcggagtg cgcgagcatg gtgaactggt cgttatcgcg
     1981 ccgtttcggc catcgatcag tctggataac ttgctgtcca gtcgcagtgc cgtgatcaac
     2041 ctgacggatg atgtgcgcat cttcgctggt gcgctcacgg gccggcgtac ttggcctgtg
     2101 aggccggccg agcgggtcag cggatatgtg cttgaagccg cgttggccca tcgcgaactg
     2161 gaattagtcg aggtcaagga tgacgtcttg cgtccggagc tctatttccg tgtcgtccat
     2221 gaggcgaatc atcgcccttt caggggattc aatcgtgccc aagccgcggt gatcgaattg
     2281 tccgtgctcg tcagcaggct acacatgcta ccgatcgaca agatcgacaa cgagcttgcc
     2341 tatctttcta tcgcgatgga aaagaccgca ggggagcgcg aaatggaagc ctggcgctgg
     2401 cttatcgagg cggtggagaa tcacagagcg gcgatccgtg gagacaatca ggcatgagcg
     2461 caccaggcca gaccagtcgc ttgctggtca gtgtatccag ccttgctgag gcggagatgg
     2521 tgatgcaggc aggggtcgat attctcgatc tcaaaaatcc gcatgcgggc gccctcggcg
     2581 cgttggaatt gtcagacatc cgttcgatcg tcaatgccac tgatgggcgc aagctggtca
     2641 gtgcgaccat cggagacgtg cctatgcaac cggcatgcat tgtggatcgg gtcgacgata
     2701 ccttgtccac gggcgtggat atcgtcaaga tcgggtttac cgatgcggca gagcgcgatg
     2761 cctgcatcgt tgcgctaggc gatattgcgc gacgaggcgc cagactcgtg gcggtgttgt
     2821 tcgcggatgc gggctatgac ctatcggtat tatccatgct gaaaacggcc gggttctatg
     2881 gggtcatgat agatactgcc gttaaagatg gtcgccacct aatggatcat atgacgctag
     2941 ctgaattgcg ggattttgtg caaaccgcac gcgaattggg cc
//
LOCUS       DATMTW010000206         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_623052, whole genome shotgun sequence.
ACCESSION   DATMTW010000206 DATMTW010000000
VERSION     DATMTW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623052"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VN084_03980"
     CDS             complement(<1..894)
                     /locus_tag="VN084_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWT28646.1"
                     /translation="MFDDGSGNWKLQINGRVQADYRAYDPSEWRDDGFTVRRARFGGT
                     FSFLKDFAVRVEGEYANDNTGARSTTAMTYAYLDYTRWKAAKLRVGQFKPFFGLERTY
                     STNFTDFIELSLATNNGSIFNSTYDRGVMVHGDPLPWLNYNAYVVNGSGQNNDDKNDG
                     KDLGGRVNANFAQLAGIRDTVIHVGASASKGDVGKTSLTQVTDGNGAQFFNVTGLDER
                     ADRDRWGVESALAYGAVKLQTEYIAANFEGKKGVTAFDRDIKAWYADINWLVTGESWA
                     DAYKSGVFGRIKPKHDFDDKDG"
     gene            complement(988..1200)
                     /locus_tag="VN084_03985"
     CDS             complement(988..1200)
                     /locus_tag="VN084_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28647.1"
                     /translation="MDRKGILVRSALVVSAMAALPATAWADADTETRIRQLEALVREM
                     QQQRAEQDKQIDVLTRELVAIEHEVI"
     gene            complement(1342..1692)
                     /locus_tag="VN084_03990"
     CDS             complement(1342..1692)
                     /locus_tag="VN084_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWT28648.1"
                     /translation="MRFPALKIRLTNGPLTAMGPGKADLLDAIRETGSISAAAKRMKM
                     SYRRAWELVDEMNRCFDQPLVQTSPGGAHGGGAQLTPLGVRVLAGYRALVVKVDALAE
                     QELADIAPHFKSPD"
     gene            1915..2133
                     /locus_tag="VN084_03995"
     CDS             1915..2133
                     /locus_tag="VN084_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28649.1"
                     /translation="MILIFFGLLLALLPLVLTTSFPHLVVVSVYTGLAHVLALAWAVF
                     ILTIIARRQRIADWRAARKRNKGKNKKA"
BASE COUNT          437 a          714 c          669 g          522 t
ORIGIN      
        1 accgtccttg tcgtcgaaat cgtgtttcgg cttgatgcgg ccaaacacgc cgctcttgta
       61 ggcatcggcc caggattcgc cggtcaccag ccagttgatg tctgcgtacc aggccttgat
      121 atcgcggtcg aacgccgtaa cgcctttctt accctcgaaa ttggcagcga tgtattcggt
      181 ctgcagcttt acagcgccgt aggccagcgc gctctccaca ccccagcgat cacggtcggc
      241 gcgctcgtcg agaccggtga cgttaaagaa ctgggcgccg ttaccgtctg tcacctgcgt
      301 cagtgaagtc ttgccgacat cgcccttgct tgccgaagcg ccgacatgga tgaccgtatc
      361 gcggatgccg gcgagctgcg cgaagttggc gttgacacgg ccgcccaggt ccttgccgtc
      421 gttcttgtcg tcattgttct gaccgctgcc gttcaccacg taggcgttgt agttgagcca
      481 tggcaatggg tcgccatgca ccatgacgcc gcggtcatag gtcgaattga agatactgcc
      541 gttgttggtg gcgagcgaca gctcgatgaa gtcggtgaaa ttggtgctat aagtccgctc
      601 caggccgaag aacggcttga actgaccgac acgcagcttg gccgccttcc agcgcgtgta
      661 atcgagatag gcataggtca tggcggtggt cgatctggcg ccagtgttgt cgttggcgta
      721 ttcaccctcg acgcgcacgg cgaaatcctt gaggaagctg aaggtgccgc cgaaacgtgc
      781 acggcgaacg gtgaagccgt catcacgcca ttctgagggg tcataagcac ggtaatcggc
      841 ctgcacgcgg ccgttgatct gcagcttcca attgccgcta ccgtcatcga acaccaggcc
      901 gtccttgaag gaagcataga cgggcgcgcc actgcgtttc tctttgcctt ccgatccggc
      961 ctgctgttcc ggcttgctgg tcttggccta gatcacctca tgctcgatcg ccaccagctc
     1021 cctggtcagt acgtcgatct gcttgtcctg ttccgcgcgc tgctgttgca tctcgcgtac
     1081 cagcgcttcc agctgacgga tccgtgtctc ggtatccgca tctgcccagg cggtcgccgg
     1141 caacgccgcc atggccgaga cgaccagggc acttctgacc aatatgcctt tccgatccat
     1201 tgtgattccc ccttgatgaa cgcccattga tgaacgttga gctgccgcga tgtcatggtc
     1261 gacctatagt gcgcaacaga tgatgccgct attttcattt gggaatagcg aagaagtcaa
     1321 caaggtttga gggaacgcca gtcaatccgg ggatttgaaa tgcggtgcga tgtcggccag
     1381 ctcttgctca gccagcgcgt cgaccttgac gaccagggca cgatagcccg ccagcacacg
     1441 cacgcccagc ggggtcaatt gcgcaccacc gccatgcgcg ccgcccgggg aagtctgcac
     1501 cagcggctgg tcgaaacagc ggttcatctc atcgaccagc tcccaggctc ggcggtagga
     1561 catcttcatg cgcttggcgg ccgcggagat ggaaccggtc tcgcggatcg cgtcgagcag
     1621 gtcggccttg cctggcccca tggcggtcag cgggccgttc gtcaatcgga tcttgagggc
     1681 tgggaatcgc ataccgcgat ttaagcataa ccgccgggct aaggcatcct tggtggcgat
     1741 tcgcatcgca taccggctac tcctacttgt aaaatccggt agcgtctcca tctgcatcat
     1801 tcgccgcacc agccatcaag ctggcaagcc ccttgcttgg gttcgatgga tgattgcagt
     1861 aagctggcgc tgcctgtttc cgatgccagc caacgaaaga taccccgact tgccatgatc
     1921 ctgatcttct tcggactgct gcttgccctc ctgcccctgg tcctgacaac ctcgttccct
     1981 cacctggtgg tcgtcagtgt ctatactggc ctggcgcatg tgctggcgct cgcctgggct
     2041 gtcttcatcc tgactattat cgcgagacgg cagcgcatcg ctgactggcg cgccgcgcgc
     2101 aaacgcaata agggcaaaaa caaaaaagcc tagcgacgcc aggctttttt gtagttactt
     2161 caaacaccag acatcagtca tggtggcagt tgcccttctt ggcctggccc ggtgggcagc
     2221 catgcgggtg atggtgatga tagaaacagc cgctcaggaa tgcactcatt gccagaacca
     2281 tgatccagga tttcatacgc tcgctcctta ttgggtgaat tattgttttc gctcgccagg
     2341 ac
//
LOCUS       DATMTW010000207         8882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_18738, whole genome shotgun sequence.
ACCESSION   DATMTW010000207 DATMTW010000000
VERSION     DATMTW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18738"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VN084_04000"
     CDS             <1..1061
                     /locus_tag="VN084_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28650.1"
                     /translation="TAQHLGLPPSCVLAISAQKALLAKIRHDEALLEKSGIGKVEQLL
                     AESVVGAKHEILSKTIIAETSGMLRASRKVAQQRVDELKAQRKELDSLKGQNRMVIQS
                     MLGKVTEERKHYEASVVTFNKAEQQINGLGQVLLNQLSLQNLDALLQVSKAEIGESWT
                     TLGLTRGMRDLMQRTAELAQLITEQAGQIKDTADEIYKLFHEHHGFPERKPPSLQVEH
                     FIHNMQQLQKATQEFCASPVNVMTEKRFLIRRFFMQLGGRAQTIFQQAHTDCQHWVRD
                     VFGPLRQQITDHKAALEKRSDSLMSIHENQHLLEASLAEMEKQLALAQHQSNDLDQML
                     LRLIKAAKPVAQPAEIIS"
     gene            complement(1133..2584)
                     /gene="rng"
                     /locus_tag="VN084_04005"
     CDS             complement(1133..2584)
                     /gene="rng"
                     /locus_tag="VN084_04005"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HWT28651.1"
                     /translation="MSEEILINVTPQETRVAVMEQGVVQELQIERNTSLGLVGNIYRG
                     EVCRVLPGMQSAFVEIGLQRAAFLHVADILECSVPEHEKTIQEVLHEGQPLIVQVIKE
                     PIGTKGARLTTQISIAGRFLVFLPQQQHIGVSQRIEDEAEREFLRERLLRLLPADHEG
                     GYIIRTMSETATDAELLADIDYLQKVWSNIHSASRDAAPRSLVFQDLSLPMRILRDVV
                     NEDTGRILIDSRETYQKAMEFAGHYAGEATRKLAHYQSDRPLFDMFNVEHEINKAMSR
                     KVELKSGGYLIFDQTEALTTVDVNTGGFVSGRSLSDTIFKTNLEAAQCIARQLRVRNL
                     GGIIIIDFIDMDEDAQRLAVLEELKRGVAKDRARIGINGFSALGLVEMTRKRTRESLA
                     HILCEPCPSCKGRGALKTAQTVCYEIFRELLRHAKQFSAREFRVLASQKVIDLLLDEE
                     SQSLANLSDFIGKPISLQVESQYAQESYDIILM"
     gene            complement(2735..3325)
                     /locus_tag="VN084_04010"
     CDS             complement(2735..3325)
                     /locus_tag="VN084_04010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWT28652.1"
                     /translation="MRKRIYLASRSPRRAELLQQIGIAFEVLPSDIDESVRHDESPAE
                     YVLRLAGEKAAACLAALQANKVELLPILAADTTVSVDGLILGKPESDEEARAMLRRMS
                     GQTHEVHTGIAVATAGGMQTALSTTHVRMATLSEAEIAAYVTSGEPFDKAGAYGIQGL
                     AGTFIAHIEGSYSGVMGLPIFETAQLLKRVGIDVLA"
     gene            3406..4041
                     /locus_tag="VN084_04015"
     CDS             3406..4041
                     /locus_tag="VN084_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HWT28653.1"
                     /translation="MIDWAYLFKIGIALFAIVNPIGSVPIFISATEGWSTQDRARTAK
                     TVAITVFMVLLVSALIGDQILQFFSISIPSFQVGGGILLMLIAISMMHGKPSHVRQTP
                     EEAQEAAEREVVAIVPLSIPLLAGPGAISSMIIAAQYNHTVLGHAALAIPIAVICCLI
                     WAALRLSVPIALKLGKTGINVVTRLMGLILAAMAVEFIAHGIEGLFPGLAS"
     gene            4096..4542
                     /locus_tag="VN084_04020"
     CDS             4096..4542
                     /locus_tag="VN084_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWT28654.1"
                     /translation="MTIWKPNTTVAAIIERDGRFLLVEEETAEGVRLNQPAGHLEDTE
                     TLIEGVIRETREETAYEFTPDALLGIYHWRHPGKDITYLRFAFTGKLGQHHADQPLDT
                     GILRAVWMSPDEIRACRSIHRSPQVMTCIEHYLAGQRYPLDVVTHL"
     gene            4552..5640
                     /gene="mnmA"
                     /locus_tag="VN084_04025"
     CDS             4552..5640
                     /gene="mnmA"
                     /locus_tag="VN084_04025"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWT28655.1"
                     /translation="MSQNKRVIVGMSGGVDSSVAALLLKEQGYDVVGLFMKNWEDDDT
                     DEYCSSKQDLIDAVSVADRIGIEIEAVNFSAEYKERVFANFLAEYSAGRTPNPDILCN
                     AEIKFKAFLDHAMGLGGDLIATGHYAQVREKDGLFQLMKADDGSKDQSYFLYRLNQAQ
                     LSKTLFPLGHLLKREVREIARKAKLPTSEKKDSTGICFIGERPFREFLNRYLPRQPGE
                     MQTPEGKVVGEHEGLMYYTYGQRQGLGIGGSRESSGEPWFVAGKDMQRNVLIVVQGHD
                     HPLLLNDGLVASQLHWIAGHAPQTHWVYAAKTRYRQPDAPCEIDRLEGDEVEVRFGQP
                     QWAITPGQSAVIYESNVCLGGGIITRAL"
     gene            complement(5732..6343)
                     /locus_tag="VN084_04030"
     CDS             complement(5732..6343)
                     /locus_tag="VN084_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWT28656.1"
                     /translation="MKLLYSLTSPYARKIRVVAAEKRIELEMVQVVLADPDCPVTHYN
                     PLGKIPVLVLDDGESLYDSPVIAEFLDNRTPVARLIPQDHTLKIRVRRWEALADGVCD
                     AAVAAIMEGRRAEAARDSAVIDKQLGKVERGLAALNAELGEGKWCVDDTFSLADIALG
                     CSLGYLQLRYQHLDWQTRFPNLARHYTAMLQRPSFSETMPPEA"
     gene            6393..7244
                     /gene="nadC"
                     /locus_tag="VN084_04035"
     CDS             6393..7244
                     /gene="nadC"
                     /locus_tag="VN084_04035"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWT28657.1"
                     /translation="MVTTRLDPALQTALEHAVRSALEEDIGAGDLTASLIPADQMAEA
                     SIVCREAAVICGIPWVDACFQAVDPGTHIDWLVSEGDKVQADQTLCRIRGRARALLTA
                     ERCALNFLQLLSGTATATRAYVDAIAGTGSRILDTRKTLPGHRLAQKYAVTVGGGMNQ
                     RLGLYDGILIKENHIAAAGGIAQALAAARAMDKTVPIQIEVESLDELKHALEAGAEMI
                     LLDNFDLDTLRAAVRLNAGRAVLEASGGVNMAHVREIAATGVDRISIGSLTKHLKAVD
                     LSMRFST"
     gene            7275..8879
                     /locus_tag="VN084_04040"
     CDS             7275..8879
                     /locus_tag="VN084_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWT28658.1"
                     /translation="MSKSATIAKPAEGRLTLGDVLRMLVNDGIVEKDQAERLYKDRKL
                     DSSNLHPLIIIAEQKWKDLRTPGKLLTSEGLTIWLAARADIEYYHVDPLKLDVGSVAQ
                     LVSKAYAERLKIMPIKSGNGEVTIATAEPFVREWIPDLERVLRMKIKLVMANPLDINR
                     YLPEIYNLANSINLANTAKAGQIVGVQNFEQLVELGKDKNLDANEQHVVNIVDWLFKY
                     AFEQRASDIHLEPRRNIGLMRFRIDGVLHQVYQLPSNIMNAITSRIKLLGRMDMVEKR
                     RPQDGRIKTLSADGLEIELRLSTMPTAFGEKLVMRIFTPEILVKNFIELGFSPAQAEQ
                     WNGWTKSPNGIILVTGPTGSGKTTTLYSTLKHLATPEVNVCTVEDPIEMVEPAFNQMQ
                     VQQNIGLTFADGVRTLLRQDPDIIMIGEIRDVETAEMAIQAALTGHLVLSTLHTNDSA
                     SAVTRLLDLGVPSYLIHSSLLGIMAQRLVRTLCPSCKKPTEVSQAAWDDLVGPWKLPK
                     PKTIYRSRSRVPAIARSSRSPRATRP"
BASE COUNT         1858 a         2776 c         2576 g         1672 t
ORIGIN      
        1 ccaccgccca gcatctcggg ctgccgcctt cctgtgtgct tgccatctcg gcgcaaaagg
       61 ccctgctagc caagattcgg catgacgagg cccttctgga aaagagtggt atcggcaaag
      121 tcgagcagtt gctggccgag agtgtggtcg gtgccaagca tgaaatattg agcaagacca
      181 tcatcgccga gaccagcggc atgctgcgtg cgtcgcgcaa ggtggcgcaa cagcgtgtgg
      241 atgaattgaa ggcgcagcgc aaggagctgg atagcctgaa aggccagaat cgcatggtga
      301 tccaatcgat gctgggaaaa gtcaccgaag aacgcaaaca ctatgaggcc tccgtcgtca
      361 ccttcaacaa ggccgaacag cagataaacg gcctcgggca ggtgttactc aaccagctca
      421 gcctgcagaa tctcgacgcc ctgttgcagg tcagcaaggc cgagatcggc gaaagttgga
      481 ccacgctcgg cctcacgcgc ggcatgcgcg acctcatgca acgcacagcc gagctcgcgc
      541 aactgatcac agaacaagcg gggcagatca aggatacggc ggatgagatc tacaagctgt
      601 tccatgaaca ccacggcttc cccgagcgca aaccgccatc gctgcaggtc gagcatttca
      661 tccacaacat gcagcagctg cagaaagcca cacaggagtt ttgcgccagc ccggtcaacg
      721 tcatgaccga gaaacgtttt ctcatccgcc gtttcttcat gcagctcggt ggacgggcac
      781 agacgatctt ccagcaggcg catacggatt gccagcactg ggtccgcgac gtgttcggcc
      841 cgcttcggca gcagatcacc gaccacaagg ctgcgctgga gaagcgcagc gattcgctca
      901 tgagcatcca tgagaaccag cacctgctgg aagccagcct ggcagagatg gagaagcagt
      961 tggcgctggc gcagcatcag agcaacgacc tcgaccagat gctgctgcgc ctgatcaagg
     1021 ccgccaaacc ggtcgcgcag cccgccgaaa tcatcagcta gccgaatctg aagccccccg
     1081 cgccatttcg atgtgccacc gcccggaagc ttcgcctgcc cttgccttgg gactacatca
     1141 ggatgatgtc gtaggattcc tgcgcatatt gcgattcaac ctgcagcgag atcggtttgc
     1201 cgatgaaatc cgaaaggttg gcgaggctct gtgattcctc atccagcaag aggtcgatga
     1261 ccttttgcga agccagtacg cggaactccc tggcgctgaa ctgcttggcg tgccgcaata
     1321 gctcgcggaa tatctcgtaa catacggttt gcgccgtctt cagcgcgccg cggcccttgc
     1381 acgacggaca cggctcacag aggatatgcg ccaggctctc acgggtgcgt ttgcgtgtca
     1441 tctccaccag cccgagggct gagaacccgt ttatgccgat gcgtgcccgg tctttggcga
     1501 cgccgcgctt gagctcttca agtaccgcca ggcgctgcgc gtcttcgtcc atgtcgatga
     1561 aatcgatgat gatgatgcca cccagattgc gcacgcgcag ctgccgggcg atgcattgcg
     1621 ctgcctcgag gttggtcttg aagatggtgt cggagaggct gcggccgctg acgaagccgc
     1681 cggtgttgac gtcgaccgtg gtcagcgcct cggtctggtc gaagatgagg tagccgccag
     1741 atttcagttc caccttgcgc gacatggcct tgttgatctc gtgctcgaca ttaaacatgt
     1801 cgaacagcgg gcgatcgctc tgataatgcg cgagcttccg cgtcgcctcg ccggcataat
     1861 gcccggcgaa ttccatggcc ttttggtagg tctcgcgcga atcgatgagg atgcgtccgg
     1921 tatcttcgtt caccacatcc cgcaatatgc gcatcggcag gctcaggtcc tggaacacga
     1981 gcgaacgtgg cgcggcatca cggctcgctg agtggatatt gctccagacc ttttgcaggt
     2041 aatcgatatc ggcgagaagc tctgcgtcgg ttgccgtctc cgacatcgtg cggatgatgt
     2101 agccgccttc atggtccgcc ggcaacaggc gtagcagacg ctcgcgcagg aattcacgtt
     2161 cggcttcgtc ctcgatgcgt tgcgagacgc cgatatgctg ctgctgcggc aggaagacca
     2221 ggaaacgtcc ggcgatgctg atctgcgtcg tgagccgcgc gcccttggtg ccaatcggct
     2281 ccttgatgac ctggacgatc agcggctggc cttcgtgcag tacttcctgg atggtctttt
     2341 cgtgctcggg cacactgcac tcgaggatat ccgccacgtg caggaaggcc gcacgctgca
     2401 ggccgatctc cacgaaggcc gactgcatgc cgggcagcac gcgacagacc tcgccacggt
     2461 agatgttgcc gaccagtccg agcgaggtat tgcgctcgat ctgcagctcc tgcaccacac
     2521 cctgctccat gaccgcgaca cgggtctcct gaggggtcac gttgatcagt atctcttcac
     2581 tcatcgttcg ttacgctttc ttgctgcaac cttatttgct tcttcattca tgccttgccg
     2641 cactgcatgt gctgtccgtg gcgttcattt gtttgcattc gcgtctcggt ttcaaccatc
     2701 caccggaaac acgcatcctg ttgatcaaga taactcaagc cagtacatcg atacccacgc
     2761 gtttaagtaa ttgagccgtt tcaaagatcg gcaaccccat gacgccagaa tagctgcctt
     2821 cgatatgtgc gatgaaggtg ccggccaaac cctggatacc ataggcgcca gctttatcga
     2881 acggttcgcc gctggtgaca taggccgcga tctctgcttc gctgagtgtc gccatgcgca
     2941 catgcgtggt cgagagtgca gtttgcatac ccccggccgt ggcaacggcg ataccggtat
     3001 ggacttcatg cgtctggccc gacatgcgcc gcaacatggc acgcgcctct tcgtcgctct
     3061 caggcttgcc gaggatcagg ccatcgacac tgacagtggt atcggccgcc agtatcggta
     3121 ataactccac cttgtttgcc tgcagcgcag cgaggcaggc ggccgctttc tccccggcca
     3181 ggcgcagcac atactccgcc ggcgattcgt catgacgcac cgattcgtcg atgtcggatg
     3241 gcaggacttc gaatgcgata ccgatctgct gcagcagttc tgcgcgacgt ggcgagcggg
     3301 aggccagata gatacgtttg cgcatgggtg ttggattcat tgccgcttgg ttgtaggctg
     3361 tgaatactaa ccgatttcaa gtacaaccga ccatcacacc acaccatgat cgactgggcc
     3421 taccttttca agatcggcat cgcgctattc gccatcgtca accccatcgg cagcgtgccg
     3481 atcttcatca gcgccaccga aggctggtcg acccaggacc gcgcgcgcac tgccaaaacc
     3541 gtggccatca cggtgttcat ggtgctgctg gtatcggccc tgattggcga ccagatcctg
     3601 caattcttca gtatcagcat tccttcgttt caagtcggcg gcggcatcct gctgatgctg
     3661 atcgccatct ccatgatgca cggcaagccc agccatgtcc ggcagacccc ggaggaagcc
     3721 caggaggcgg cggagcgcga agtggtcgcg atcgtcccct tgagcatccc cttgcttgca
     3781 ggccccggcg ccatcagcag catgatcatc gcagcgcagt acaaccacac ggtcctcggc
     3841 catgctgcgc tggcgatccc gatcgctgtc atctgctgcc tgatctgggc cgcattgcgg
     3901 ctgtcagtgc cgatcgcgct caagcttggc aagaccggca tcaacgtcgt cacgcgtctc
     3961 atgggactga tcctggccgc catggccgtc gagttcatcg cccacggcat cgaaggcttg
     4021 ttccccggtt tggcttcata atcaaggacg ttgtagaata cgcggctttt ctgcacgcag
     4081 accgcccgcc ccgatatgac catctggaag cccaatacca ctgtcgccgc catcatcgaa
     4141 cgagatggcc gtttcctgct ggtcgaggaa gaaaccgctg agggcgtgag gctcaaccag
     4201 ccggcggggc atctcgaaga taccgaaacc ctgatcgagg gcgtcatccg cgaaacgcgt
     4261 gaagagaccg cctacgagtt cacgccagac gcactgctag gcatctatca ttggcgtcac
     4321 ccgggcaagg acatcaccta tctgcgcttc gccttcaccg gaaaactcgg gcagcaccat
     4381 gccgatcagc cactggatac cggtatcctg cgcgccgtgt ggatgtcgcc ggacgagatc
     4441 cgcgcctgcc ggagcattca tcgcagccca caagtcatga cctgcatcga acattacctg
     4501 gccggccagc gttatccgct ggatgtggtc acccacctgt gagcgccatc catgagccaa
     4561 aataaacgtg tgatcgtcgg catgtccggc ggcgtcgatt cctcggtcgc agccttgctg
     4621 ctgaaagaac aaggctatga cgtggtcggc ctgttcatga agaactggga ggacgacgac
     4681 accgatgaat actgctcctc gaaacaggac ctcatcgatg ccgtatcggt cgccgacagg
     4741 atcggcatcg agatcgaagc cgtcaatttc tccgccgaat acaaggagcg cgtgttcgct
     4801 aacttcctcg ccgaatatag cgccggacgc acgccgaacc cggatatcct gtgcaatgcc
     4861 gagatcaagt tcaaggcttt ccttgaccat gccatgggcc tcggcggcga tctcatcgcc
     4921 accggtcatt atgcgcaggt gcgcgagaaa gacggactgt tccagctcat gaaggcggac
     4981 gacggcagca aggaccagag ctacttcctc tatcgcctga accaggcgca actgtcgaag
     5041 acgctattcc cgctcgggca tctgctcaag cgggaagtgc gggagatcgc gcgcaaggcc
     5101 aagctgccga ccagcgaaaa aaaggattcc acaggcatct gcttcatcgg ggaacgcccg
     5161 ttccgcgagt tcctcaaccg ttacctgccg cgccagccag gcgagatgca aacgccggaa
     5221 ggcaaggtcg tcggcgaaca cgaagggctg atgtactaca cgtacggcca gcgtcagggt
     5281 ctgggtatcg gcggctcgcg cgaaagtagc ggcgaaccgt ggttcgtcgc cggcaaggat
     5341 atgcagcgca acgtgctgat cgtggtgcaa ggtcatgacc acccgttact gctcaacgac
     5401 ggcctggtcg ccagccagct gcactggatc gctgggcatg cgccgcaaac gcattgggtg
     5461 tatgccgcaa agacccgcta tcgccagccg gatgcgcctt gtgagatcga tcggctcgag
     5521 ggggatgagg tagaggtgcg tttcggccag ccgcaatggg cgatcacacc cggccagtct
     5581 gcagtgatct acgagagcaa tgtctgcctg ggcggcggca tcatcacgcg cgcgctctag
     5641 cgcgcaaaag ctggcagcgc aaagtcagtc atcaaactga ccagataacg catactcccc
     5701 caaactccgc agcgcagatc aacgggtcgc atcaggcttc gggcggcatc gtctcgctga
     5761 aggacggccg ctgcagcatg gccgtgtagt ggcgtgccag attggggaag cgcgtctgcc
     5821 agtcgagatg ctggtaacgc aactgcagat aacccaggga gcagcccagg gcgatatccg
     5881 ccaggctgaa ggtatcgtcg acgcaccatt tgccttcacc cagttcggcg ttcaaggcgg
     5941 cgaggccacg ctccaccttg cccagctgct tatcgatcac cgcgctatcg cgcgcggctt
     6001 cggcacgcct gccttccatg atcgccgcca cagcggcatc gcacacgcca tcggccagcg
     6061 cctcccagcg acgcacgcgg atcttcagcg tgtggtcttg cggaatgagg cgcgcaactg
     6121 gcgtgcggtt atcgaggaat tccgcgatca ccggcgaatc atagaggctc tcgccatcat
     6181 cgagcaccag caccgggatc ttgcccagag ggttgtaatg agtaaccggg caatccgggt
     6241 cggcgagcac gacctgcacc atctccagct cgatacgctt ctccgcggcg accacacgga
     6301 tcttgcgtgc atagggacta gtcagggagt aaagcagttt catgtcgcgg ctcaccgtat
     6361 gggtagggat acactgcatt ataatggaaa tcatggtaac cactcgactt gacccggccc
     6421 tacaaaccgc cctcgaacat gccgtgagat cggcgttgga agaggatatc ggcgcaggag
     6481 acctgacagc gtcattgatc ccggccgacc aaatggcgga ggccagcatc gtctgtcgtg
     6541 aagcggccgt gatctgcggg atcccgtggg tcgatgcctg ttttcaggcc gtagaccctg
     6601 gcacccatat cgactggctg gttagcgaag gcgacaaagt ccaggccgac cagacgctct
     6661 gccgcatccg cggccgtgcc cgcgcgttgc tcacggccga gcgttgtgcg ctcaacttcc
     6721 tgcaactgct atcaggtacc gccacggcga cgcgcgccta tgtcgacgct atcgccggca
     6781 ccggtagccg catcctggat acacgcaaga ccctgcccgg ccaccggctg gcgcagaaat
     6841 atgccgtcac cgtcggcggc ggcatgaatc agcggctggg cctgtatgac ggcatcctga
     6901 tcaaggagaa ccatatcgcg gcggccggcg gcatcgcaca ggccctcgcc gcagcgcgag
     6961 ccatggacaa gaccgtgccg atccagatcg aggtcgaatc gctggacgaa ctgaagcatg
     7021 ccttggaggc aggtgccgag atgatcctgc tcgacaattt cgatctcgac acgctccgcg
     7081 cggctgtccg gctcaatgcc ggcagggctg ttctcgaagc ctccggtggg gttaatatgg
     7141 cgcatgtccg cgagatcgcg gcgactggcg tcgaccgcat ctccatcggc agcctgacca
     7201 agcatctgaa ggccgttgac ctctcgatgc gattcagcac ctaaacctga aagctcgaaa
     7261 aaggcgtaac gcacatgagt aaatcggcca cgatcgccaa gcctgcggaa ggcagactca
     7321 ccctcgggga tgtgctgcgt atgctggtca acgatggcat cgtggagaag gaccaggccg
     7381 aacgcctcta caaggaccgt aaactcgata gttcgaacct gcatccgctg atcatcatcg
     7441 ccgagcagaa atggaaagac ctgcgcacgc ccggcaagct gctcaccagc gagggcctga
     7501 ccatctggct ggcagcgcgc gccgatatcg agtattacca tgtcgatccg ctcaagctcg
     7561 acgtcggatc ggtcgcccag ctcgtttcca aggcgtatgc cgagcgcctc aagatcatgc
     7621 cgatcaaatc cggcaacggt gaagtcacca tcgccacggc tgagccgttc gtcagggaat
     7681 ggatccccga tctcgagcgc gtattgcgca tgaagatcaa gctggtgatg gccaacccgc
     7741 tcgatatcaa ccgttacctg cccgagatct acaacctggc caattcgatc aacctggcga
     7801 atacggccaa ggccggccag atcgtcgggg tgcagaattt cgagcaactg gtcgagttgg
     7861 gcaaggacaa gaacctcgac gccaacgaac aacatgtcgt caatatcgtc gactggctat
     7921 tcaagtacgc gttcgagcag cgcgccagcg acatccatct cgagccacgc cgcaacatcg
     7981 gcctcatgcg gttccgcatc gacggcgtgc tgcatcaggt ctaccagcta ccgagcaaca
     8041 tcatgaacgc catcaccagc cgcatcaaat tgctgggtcg catggacatg gtggaaaagc
     8101 gccgcccgca ggacggccgc atcaagaccc tgagcgccga tggcctggag atcgaactgc
     8161 gtctctcaac catgccgact gcctttggcg agaaacttgt catgcggatc ttcacgccgg
     8221 agatcctggt caagaacttc atcgaactcg gattctcccc ggcgcaagcc gagcaatgga
     8281 acggctggac caagagcccc aacggcatca tcctggtcac aggcccgacc ggctccggca
     8341 agaccaccac gctctattcc acgctcaagc atctggccac gcccgaggtc aacgtctgca
     8401 cggtggagga cccgatcgaa atggtcgagc cggcattcaa ccagatgcag gtgcagcaga
     8461 atatcggcct gacctttgcc gatggcgtac gtacgctgtt gcggcaggat ccggacatca
     8521 tcatgatcgg cgagatccgc gatgtggaga ccgccgagat ggcgatccag gcggccctca
     8581 ccggccacct ggtgttgtcg acgctgcaca ccaacgactc agcctccgcc gtcacgcgcc
     8641 tgctggacct tggcgtgccg tcctatctca tccattcctc gttgctcggt atcatggcgc
     8701 aacgcctggt acgcacgcta tgcccaagct gcaagaagcc gaccgaagta tcgcaggcag
     8761 catgggacga tctggtcggc ccctggaaac taccgaaacc gaagaccatc tatagatcgc
     8821 gttctcgggt gccggcgatc gcgagatcat cgcgttcacc gcgggcaaca agaccgtgac
     8881 ct
//
LOCUS       DATMTW010000208         4968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_355027, whole genome shotgun sequence.
ACCESSION   DATMTW010000208 DATMTW010000000
VERSION     DATMTW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355027"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..863
                     /locus_tag="VN084_04045"
     CDS             153..863
                     /locus_tag="VN084_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWT28659.1"
                     /translation="MAGTLYLLPVTLGDDRLTHALPAEVIALAQRLEIFVVENEKTAR
                     RFLGLIKPFKPVRELQLLLLNEHTTDKQLPDLLAPLLAGHDVGLMSEAGCPGIADPGA
                     RLAQLAHRKGIRVAPLVGPSSILMALMASGLNGQRFSFLGYLPSGKAERILQLKALEK
                     ASQRHAETQIFIETPYRNQHMLEDILAHCQPETALCVAANISMENESIISRTVAEWRK
                     SPLPDLHKQPAVFLLLAG"
     gene            complement(880..1452)
                     /gene="rnhB"
                     /locus_tag="VN084_04050"
     CDS             complement(880..1452)
                     /gene="rnhB"
                     /locus_tag="VN084_04050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWT28660.1"
                     /translation="MTSWLVCGVDEAGRGPLAGAVYAAAVILDPARPISGLDDSKKLS
                     EKQRDRLAIEIKQSALAWAIASSSVEEIDEINILQASLLAMRRAVDGLQRVPQLVQVD
                     GLHCPVVSMPVEAIVQGDSKVPAIAAASILAKTARDAEMLRLHALYPQYDFCRHKGYP
                     TAHHLQCLQLHGVSPVHRRSYAPIRKLLSR"
     gene            complement(1449..2585)
                     /gene="lpxB"
                     /locus_tag="VN084_04055"
     CDS             complement(1449..2585)
                     /gene="lpxB"
                     /locus_tag="VN084_04055"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWT28661.1"
                     /translation="MPKVGIVAGEASGDLLGSHLIRALKKHRPDIEFIGIAGPKMQSE
                     GARSLFPIERLSVRGYVEVLRHLPGLLKIRSELAQFFLKTKPDLFIGIDAPDFNFGLE
                     KKLKRKGILTIHYVSPSIWAWRKGRIGKIRRAVSHMLALFPFEPEIYDQAGIPVTYVG
                     HPLADILPMTPDKQGARERLKLKEDKLVIAMLPGSRQSEVKQLADLYVKTAKLIAQEQ
                     PEVKFLVPLITRETRAIFERAIYANQADETPIQILFGHAHMAMEAADAVIVASGTATL
                     EAALLKRPMIITYRMPRISWWILKRMNYLPYVGLPNILAGRFVVPELLQNDAVPEKLA
                     EATLRMVSDKVLMQEIGAEFSRIHEALRQNTEEKAAQAILAYLA"
     gene            complement(2594..3373)
                     /gene="lpxA"
                     /locus_tag="VN084_04060"
     CDS             complement(2594..3373)
                     /gene="lpxA"
                     /locus_tag="VN084_04060"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWT28662.1"
                     /translation="MTSLIHPTAIVDPRAELDSSVEVGPYSIIGPDVRIDAGTRVGSH
                     VVIKGPTTIGKHNQIFQFSSLGEQPQDKKYRDEPTTLEIGDNNTIREFCTFNRGTVQD
                     KGATRIGNDNWIMAYVHIAHDCQIGNHTIFANNSSLAGHVDVHDYAILGGFTLIHQFC
                     KIGAHIITAVGSVVFKDIPPYVTAAGYDAKPHGINSEGLKRRGFSPEAILQIKRAYKT
                     LYRNGLTLEQAKQELAQQVGQCAELGILLEFLNTSTRGIVR"
     gene            complement(3429..3896)
                     /gene="fabZ"
                     /locus_tag="VN084_04065"
     CDS             complement(3429..3896)
                     /gene="fabZ"
                     /locus_tag="VN084_04065"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWT28663.1"
                     /translation="MTEQAIMSMDIHEILAHLPHRYPFVLVDRVLSLEVGKEIVAVKN
                     VSVNEPYFPGHFPYHPVMPGVLIVEAMAQAAAILSFKTMDTKPSDDSVYYFAGIDSAR
                     FKKPVSPGDQLILKVKIDRILRGIWKYSGQALVDDNVVAEAEMMCILKAIDDK"
     gene            complement(3905..4960)
                     /gene="lpxD"
                     /locus_tag="VN084_04070"
     CDS             complement(3905..4960)
                     /gene="lpxD"
                     /locus_tag="VN084_04070"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWT28664.1"
                     /translation="MKAQYSLREIVSILGGEVLGDADTVITRVASLANAQAGHISFLS
                     DSKYKSQLAKTLASAIILAPAHRDATDLPRILTDNPYAYFARISELLNPLSVTVPGIS
                     ASAVISQTATVPASCSIGAHAVIGDHVVLGERVVIGAGCVLGDHVRIDEGSQLAPNVV
                     IYQGCVIGKQCQLFAGVVIGADGFGYAEDGGRWVKIPQVGRVVIHDHVDIGANTTIDR
                     GAIDDTVIEEGVKLDNLIQIGHNCRIGAHTVIAGCVGIAGSAKIGQHCRIGGAAMILG
                     HLEIADGVTISPGSMITRTITRAGTYTALMPFLPHEDWLRTAASLRHVGEYAERIRKL
                     EKALAALVHEQDNTEKN"
BASE COUNT         1015 a         1449 c         1414 g         1090 t
ORIGIN      
        1 gtgatcgtgc aagaaccgat ccccggcgat tggaagatgt tgcgatccaa tctgacccac
       61 gagaaagctt ccagcaatat ggccgcatgg aagatcgaca tcccggctga aggtcaggca
      121 acactcaatt atcgcgtgca agtcaaatac tgatggccgg cacgctctac ctgttgcccg
      181 tcaccttggg cgacgaccgc ctgacgcatg cgttgcctgc cgaggtcatc gcccttgccc
      241 agcggctaga gatctttgtc gtggagaacg aaaagacagc gcgccgtttt cttggtctta
      301 tcaaaccctt caagccggtg cgcgagttgc aattattgtt gctgaacgag catacaacgg
      361 acaagcaact ccccgatcta ctggcgcctt tgctggcggg tcatgatgtc ggcctcatgt
      421 ccgaggcggg ttgcccgggg atcgccgacc cgggggcacg gcttgcccag cttgcgcatc
      481 gcaagggaat aagagtggcc ccactggtag gcccctcttc catcctgatg gccctgatgg
      541 cttctggcct gaatggtcag cgctttagct ttctcggcta tctgccgtca ggcaaggcgg
      601 aacgtatcct gcaactgaag gcgctggaaa aagcatccca acggcacgcc gagacccaga
      661 tctttatcga gaccccatac cgtaaccagc acatgctgga agacatcctg gcccactgtc
      721 agccagagac tgccttgtgc gtggccgcga acatcagcat ggagaacgag tcgatcatca
      781 gccgcaccgt cgcagaatgg cgcaaatcgc cattacctga tttgcacaag caacctgcgg
      841 tcttcctgct attggcaggc taacaccacc accactatct cagcgcgaca ataacttgcg
      901 gatcggggcg tagcttcgcc tatgcaccgg cgagacaccg tgcaactgta ggcattgcag
      961 gtgatgggcg gtaggataac ccttgtggcg gcaaaaatcg tactgcggat acagtgcatg
     1021 cagtcgcagc atttcggcat cacgcgccgt cttggccagt atcgatgccg cagcgatggc
     1081 tggaaccttg ctgtcgccct gcacgatggc ttctaccggc atgctgacca cggggcaatg
     1141 caggccatct acctgaacca gctgcggtac gcgctgcaac ccatcgactg cgcgacgcat
     1201 ggctaacaga ctggcctgca ggatgttgat ctcatcgatc tcctccacac tgctgctggc
     1261 aatggcccat gccagggccg attgcttgat ctcgatcgcc agccggtcgc gctgcttctc
     1321 tgaaagtttt ttcgaatcgt cgagcccgga gatgggcctt gccggatcga gtatgacggc
     1381 tgcagcgtag acggcaccag cgagtggacc tcgcccggcc tcatcaacac cacagaccag
     1441 ccatgatgtc atgccagata ggccaggatg gcctgggccg ctttttcttc ggtgttctgg
     1501 cgcagtgcct cgtggatacg gctgaactcg gcaccgatct cctgcatcaa caccttgtcg
     1561 ctgaccatgc gcaacgtagc ttccgccagc ttttctggga ctgcatcgtt ctgtagcagc
     1621 tcaggcacga cgaagcgacc agcaaggatg ttaggcaacc cgacatatgg caggtaattc
     1681 atgcgcttga gtatccacca gctgatccgt ggcatgcggt aggtgatgat catgggccgc
     1741 ttgagcagcg cggcttccag cgttgccgta ccggaggcaa cgatcacggc gtcagccgct
     1801 tccatcgcca tgtgtgcgtg accgaacaat atctggatcg gggtctcatc cgcctggttg
     1861 gcatagatcg cccgctcaaa gatcgcacgc gtctcgcgtg tgatgagcgg caccagaaat
     1921 ttcacctccg gctgttcttg tgcgatgagt ttggcggtct tcacgtagag atcagccaac
     1981 tgtttgacct cgctctgccg gctgccaggc agcatggcaa tcacaagctt gtcttccttc
     2041 agtttcaggc gctctcgcgc accttgcttg tctggcgtca tcggtaggat atccgccagc
     2101 ggatgaccca cataagtgac cggtatccct gcctggtcat agatctcagg ctcaaagggg
     2161 aacagcgcca gcatgtgcga taccgcgcgc ctgatcttgc cgatgcggcc tttacgccag
     2221 gcccagatcg aagggctcac ataatggata gtaaggatcc ccttgcgctt gagttttttt
     2281 tccagcccga aattgaaatc cggggcgtcg atgccgatga acagatcagg tttggtcttg
     2341 agaaaaaact gcgcgagttc gctacggatc ttgagtaggc cgggcagatg ccgtaacact
     2401 tcgacatagc cacggaccga taggcgctcg atcgggaaaa gcgagcgtgc gccctcgctc
     2461 tgcatcttgg gacccgcgat gccgatgaac tcgatatcag gccgatgctt cttcagcgca
     2521 cggataagat ggctgccgag tagatcgcca gaggcttctc cggccacgat gccgacttta
     2581 ggcatgttga ttcctaacgg acgatgccgc gggtcgaggt attgaggaat tccagcagga
     2641 tgccgagttc ggcacactgg cctacctgtt gcgcgagttc ctgcttggcc tgctccagcg
     2701 tcagcccatt ccgatacagc gtcttgtagg cacgcttgat ctgcaagatg gcctcagggg
     2761 agaaaccgcg tcgtttaagt ccttcgctgt tgatgccatg tggcttggca tcataaccgg
     2821 cagccgtcac atacggcggg atatccttga acacgaccga ccctacggca gtgatgatat
     2881 gggcgccgat cttgcagaac tgatggatca acgtgaatcc gccgaggatc gcataatcgt
     2941 gcacatcgac atgccccgcc agcgatgaat tgtttgcgaa gatggtatgg ttgccgatct
     3001 ggcaatcatg ggcaatatgc acataggcca tgatccagtt gtcattgccg atgcgcgtcg
     3061 cccccttgtc ctgcaccgtg ccacggttga aagtgcaaaa ttcgcgaatg gtgttgttgt
     3121 caccgatctc aagcgtagtc ggctcgtcac ggtatttctt gtcctgcggt tgctccccaa
     3181 gcgaggagaa ctggaagatc tggttgtgct tgccgatcgt cgtcggcccc ttgatcacga
     3241 catggctgcc gacgcgtgta ccggcatcga tacgcacatc ggggccgatg atgctgtaag
     3301 gcccgacttc cacgctggaa tcaagctcgg cccgaggatc gacgatcgca gtgggatgga
     3361 tgagggatgt catacggttc ttgttctgat tggcgggttg ttcgtcgttc acttcgcttg
     3421 ctgatcgctc acttatcgtc gatggctttc aagatgcaca tcatctcggc ctcggccacg
     3481 acgttgtcat cgaccagcgc ctgaccggag tacttccata taccgcgcag gatccggtcg
     3541 atcttgacct tgagaataag ctggtcacca ggggacactg gcttcttgaa acgggcactg
     3601 tcgataccgg cgaagtagta caccgaatcg tcactgggct tggtgtccat ggtcttgaag
     3661 gacaggatgg cagcagcctg ggccatggct tcgacgatca gcacgcccgg catcacaggg
     3721 tgatatggga agtgcccggg gaaatatggt tcgttcacac tgacgttctt caccgccacg
     3781 atctccttgc cgacctccag ggacagaacg cgatcgacca gtacgaaagg ataacggtgc
     3841 ggtagatgcg ccaggatctc gtgtatgtcc atgctcatga tggcttgctc agtcatgctc
     3901 ttcctcagtt cttctcagta ttgtcttgtt cgtgcacgag cgcagccaat gctttttcca
     3961 gcttgcggat acgctcggca tattcgccaa cgtgacgcag gcttgctgcg gtcctcagcc
     4021 agtcctcatg tggcaggaat ggcatcaggg cggtataggt tccagcccgg gtgatcgtcc
     4081 tcgtaatcat actgcctggc gatatggtca cgccatcggc aatttccaga tgccccagta
     4141 tcattgctgc accgccgatc ctgcaatgct ggccgatctt cgcgctaccg gcgatgccaa
     4201 cgcagccggc gatgaccgta tgggcaccga tccggcagtt gtgtccgatc tggatgaggt
     4261 tgtcgagctt gacgccctcc tcgatcaccg tgtcatcgat cgcgccacga tcgatggtgg
     4321 tgttggcacc gatatctaca tggtcatgga tcacgacgcg cccgacctgc gggatcttga
     4381 cccagcggcc gccatcctcg gcgtagccga aaccatcggc gccgatgacg acgccggcga
     4441 acagctggca ttgcttgccg atgacacagc cctggtagat cactacgttg ggagcaagct
     4501 ggctgccttc gtcaatacgc acgtgatcgc cgagcacgca tccagcgccg atcacaacgc
     4561 gctcacccag taccacatgg tcgccgatga cagcatgcgc accgatactg caactggctg
     4621 gcacggtcgc tgtttggctg atcactgcgc tcgcggaaat cccggggaca gtcacgctca
     4681 acggattgag cagctccgat atcctggcga aatacgcata tgggttatct gtgaggatgc
     4741 gaggcaagtc ggtcgcgtcg cgatgcgctg gcgccaggat aatggcgctg gctagggtct
     4801 tcgccaattg tgacttgtat ttgctgtcac tgagaaagct gatatgcccg gcctgggcat
     4861 tggcaagtga ggcgacgcgc gtaatgacag tatcagcgtc acccaacact tcgcctccca
     4921 gtattgatac gatctcgcgt aaggaatatt gcgctttcat ccgacgaa
//
LOCUS       DATMTW010000209         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_18949, whole genome shotgun sequence.
ACCESSION   DATMTW010000209 DATMTW010000000
VERSION     DATMTW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18949"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VN084_04075"
     CDS             complement(<1..1217)
                     /locus_tag="VN084_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT28665.1"
                     /translation="MAKTVPDKPATQPTATSGPSLEEMNAIVALFQAADWTGLETSSR
                     AMTEQWPLHGFGWKSLGVALKMQKRDDQALQVMRKAAALMPNDPDVHNNLASALREAG
                     DHTQAIAHFEQALALNPDFPDALNGLAITLKQSGDPQRVIALLKRAIALRPTFTEAHY
                     NLGNAIKAAGDLDEAVKSYMEALKLRPGYADAYNSLGNALMQKGEPEEALACYQQSLT
                     ADPAFHEAYNGRGNALRALGRLDDAISSYQEALRLKPGHEGFHHNLAIAYSEKMQVDA
                     ALACYRESLRIAPDYASSRAGLGMLLIANGQMEEGWPLYESRWEGFQQAMEGLLKRPE
                     TALPQWRGEPVGATDALLLFCEQGLGDSLQFIRYVPLLARIFAQVTVVCPGAVLNIFR
                     GSFQLPNVEFIES"
     gene            complement(1220..2065)
                     /gene="rlmJ"
                     /locus_tag="VN084_04080"
     CDS             complement(1220..2065)
                     /gene="rlmJ"
                     /locus_tag="VN084_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HWT28666.1"
                     /translation="MLSYRHAFHAGNHADVLKHYVLTQVLDYASGKEKPLWYIDTHAG
                     AGMYALDQGYASQNAEHAGGIGRLWSATDLPPSLAEYVAMVKGMNPDHAMRFYPGSPQ
                     VAQALLRKEDRIRLFELHPTDAKLLRQRFKDAGRQVMIETTDGFAGLKASLPPPSRRA
                     VVLIDPPYEEKQDYQSVVRAIEDSLQRFATGTYILWYPLLQRPEPLQMLERLARLPLK
                     SWLHVSLTVQQPSIDGFGMYGSGLFIVNPPWTLPALLAESMPILVKKLGLDSDADYRL
                     EHHIP"
     gene            complement(2107..>2378)
                     /locus_tag="VN084_04085"
     CDS             complement(2107..>2378)
                     /locus_tag="VN084_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HWT28667.1"
                     /translation="GLGAGGHFDPDKSDHHAGPEGDGHHGDLPALEVAEDGSATRILL
                     APRLKLEDIKGRSLMIHEGGDNYSDTPKPLGGGGARVACGIIDQD"
BASE COUNT          406 a          671 c          798 g          503 t
ORIGIN      
        1 tgggattcga tgaattcgac attgggtaac tggaaggagc cgcggaagat attgagcacg
       61 gccccgggac agaccaccgt gacctgcgcg aagatacggg ccagtagcgg cacataacgg
      121 atgaactgca ggctgtcgcc cagaccctgt tcgcagaaca gcagcagggc gtcggtcgcg
      181 ccgaccggct cgcctctcca ttgcggcagg gctgtctcag gccgtttgag caagccttcc
      241 atggcttgct ggaaaccttc ccaacggctc tcgtagagcg gccagccttc ctccatctgg
      301 ccgttggcga tcagcagcat gccgaggccg gcacgcgatg aagcatagtc aggggcgatg
      361 cgcagtgatt cgcggtagca ggcgagcgcc gcgtcgactt gcatcttctc gctgtaagcg
      421 atggccagat tgtgatggaa gccttcgtgc ccgggcttga gtcgtagcgc ttcctgatag
      481 ctgctgatgg cgtcgtcgag ccggccgagc gcgcgcaggg cgttgccgcg gccgttatag
      541 gcctcatgga acgccggatc cgcagtcaat gattgctgat agcaggcgag tgcttcttca
      601 ggctcgcctt tttgcatcaa ggcattgccc aggctgttgt aggcgtcggc atagccgggt
      661 cgcagcttga gcgcttccat gtagctcttg acggcttcgt cgagatcacc cgcggccttg
      721 atggcattac ccagattgta atgagcctcg gtgaaggtcg ggcgtaaggc gatcgcacgt
      781 ttcagtaagg cgatcacgcg ctgcggatca cctgactgct tgagtgtgat ggcgaggccg
      841 ttgagtgcat ccgggaaatc cgggttcagc gccagggctt gctcgaagtg ggcgatggcc
      901 tgcgtatggt cgccggcttc acgcaaggca ctggcaaggt tgttatgcac atccggatcg
      961 ttcggcatca gtgcggcggc cttgcgcatc acctgcagcg cctggtcgtc gcgtttttgc
     1021 atcttgagcg cgacacccag cgatttccag ccgaagccgt gtaatggcca ttgttctgtc
     1081 atggcgcgcg agctggtttc aagcccggtc cagtccgctg cctggaacag cgcaacgatg
     1141 gcgttcatct cctcgaggct cgggccgctg gttgcggtcg gctgcgtagc cggcttgtcg
     1201 ggcacggtct tggccatggt caggggatgt gatgctccag gcggtaatca gcgtcgctgt
     1261 cgaggccgag ctttttgacc aggatgggca tgctttccgc aagcagggcg ggcagggtcc
     1321 aggggggatt gacgatgaac aggccactgc cgtacatgcc gaacccgtcg atggacggct
     1381 gctgcacggt gagtgatacg tgcagccaac tcttcagcgg caagcgggcg agtctttcca
     1441 gcatttgcag gggttcaggc ctttgcaaca ggggatacca gagtatatag gtgcccgtgg
     1501 cgaaacgctg caagctatcc tcgatggccc tgactacgct ctggtaatcc tgtttttcct
     1561 cgtagggcgg atcgatcagt acgacggcac gccgcgaagg cggtggcaga ctggccttga
     1621 ggccggcgaa accgtccgta gtctcgatca tgacttgtcg ccctgcgtcc ttgaagcgct
     1681 ggcgcagcag cttggcatcg gtggggtgca gttcgaacag acggatcctg tcctccttgc
     1741 gcagcaatgc ctgggcgacc tgcggtgagc cggggtagaa gcgcatggcg tgatcggggt
     1801 tcatgccttt gaccatagcg acgtactccg ccagggaggg tggcaggtct gtggccgacc
     1861 acaggcggcc gatgccgccg gcatgctctg cattctggct ggcgtagcct tgatccagcg
     1921 cgtacatgcc ggcgccggca tgcgtgtcga tataccagag tggcttttcc ttgcctgagg
     1981 cgtagtccag cacttgtgtc aggacatagt gtttgaggac atcggcatgg ttgccggcgt
     2041 gaaaggcatg gcgatagcta agcatccgct tatgagtccc cggcaggtga tagagaggtg
     2101 gtggtgttag tcttggtcga tgatgccgca ggcgacacga gcgccgccac cgcccagcgg
     2161 tttgggcgtg tcactgtagt tatcgccacc ctcgtggatc atcagtgaac ggcccttgat
     2221 gtcttcgagc ttgaggcgcg gcgcgagcag gatgcgtgtc gcactgccgt cttcagcgac
     2281 ttccagtgct ggcaggtcgc catggtggcc atcgccttcc gggccggcat ggtggtcgct
     2341 tttgtccgga tcgaagtgac caccggcgcc gagtccgg
//
LOCUS       DATMTW010000210         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_152325, whole genome shotgun sequence.
ACCESSION   DATMTW010000210 DATMTW010000000
VERSION     DATMTW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152325"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..685
                     /gene="leuD"
                     /locus_tag="VN084_04090"
     CDS             47..685
                     /gene="leuD"
                     /locus_tag="VN084_04090"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWT28668.1"
                     /translation="MQPFVTLDGLAVPLDRANVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSQRPLNPDFVLNQPRYQGAEVLLARENFGCGSSREHAPWALLDY
                     GFRAIVAPSFADIFFNNCFKNGILPVVLPAEAVDKLFKLVDAHPGCRITIDLPQQQVT
                     TADGQAYSFEVDAFRKHCLLNGLDDIGLTLQHVDGIKAFEAKHKQSQPWLFN"
     gene            724..1794
                     /gene="leuB"
                     /locus_tag="VN084_04095"
     CDS             724..1794
                     /gene="leuB"
                     /locus_tag="VN084_04095"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWT28669.1"
                     /translation="MKIAVLPGDGIGPEIVAQAVKVLKALNLDMQLEEAPIGGAGYEA
                     AGDPLPEATLKLAKSSDAVLLGAVGDWKYDSLPREQRPERGLLRIRKELNLFANLRPA
                     LLYPELASASTLKPEVVSGLDLMIVRELTGDIYFGQPRGISTLANGEREGINTMRYNE
                     SEIRRIGHVAFGIAMKRRKDGQKAKVCSVDKANVLETTELWRQVMIEVAKEYPEVELS
                     HMYVDNAAMQLVRAPKQFDVMVTGNIFGDILSDEASMLTGSIGMLPSASLDANNKGMY
                     EPSHGSAPDIAGKDIANPLATILSAAMMLRYTFNDEANALRIENAVKKALAEGYRTAD
                     IHTDGCRKVKCSEMGDAVVAAL"
     gene            1831..>2200
                     /locus_tag="VN084_04100"
     CDS             1831..>2200
                     /locus_tag="VN084_04100"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWT28670.1"
                     /translation="MLKVGFIGWRGMVGSVLMQRMREENDFALIEPVFFTTSQPGGKG
                     PDVGRDIPPLKDAKNIAELKAMDVIISCQGGDYTTEIFPQLRKAGWNGHWIDAASTLR
                     MEKDSVIILDPVNRNVIDQAL"
BASE COUNT          500 a          612 c          652 g          436 t
ORIGIN      
        1 aagccgtgca gcgcaacagt aaataacgag actcgaaaca gacgctatgc aaccttttgt
       61 gaccctggat ggattggccg tcccactcga ccgtgcgaac gtcgataccg acgccatcat
      121 ccccaaacag ttcctcaagt cgatcaaacg ctcgggcttc ggtcccaatg catttgacga
      181 atggcgctat cttgaccatg gggagcctgg catggataac agccagcgcc ccctgaatcc
      241 ggattttgtg ctcaaccagc cacgctatca gggcgcggaa gtgctgctcg cccgcgagaa
      301 cttcggttgc ggatcgagcc gggaacatgc gccatgggcc ttgctcgact atgggttccg
      361 cgccatcgtc gcgccgagct tcgccgatat ctttttcaat aactgcttca agaacggcat
      421 cttgcccgtc gtattgccgg ctgaggcggt agacaagctg ttcaagctgg tggatgcgca
      481 tccgggttgc cggatcacga tcgatctgcc acagcagcaa gtcactacgg cagatggcca
      541 ggcatacagc ttcgaggtgg atgctttccg caagcattgc ctgctcaatg gcctggatga
      601 catcgggctc accctgcaac atgtcgacgg gatcaaggcc ttcgaggcca agcacaagca
      661 atcacagccc tggctcttca attagcgggc tgttcaacaa gacatcaaaa ggaatcagat
      721 tagatgaaga tcgcagtatt gccaggggat ggcatcggtc ccgagatcgt ggcgcaggcc
      781 gtgaaggtgc tcaaggcgct gaatctcgat atgcagctgg aagaagcccc tatcggcggc
      841 gccgggtatg aagccgcagg cgacccgctg ccggaagcga cgctcaagct agccaagtcc
      901 tccgatgccg tgctgctggg cgcggtcgga gactggaaat acgacagcct gccacgcgag
      961 cagcgtccgg agcgcggtct gctgcgtatc cgcaaggaac tcaacctgtt tgccaatctg
     1021 cgtccggctt tgctataccc cgagttggct tcggcatcga ccctgaaacc agaagtcgtc
     1081 tcgggtctcg acttgatgat tgtgcgtgaa ttgaccgggg atatctattt cggccagcca
     1141 cgcggcataa gcacgctggc gaacggcgaa cgcgaaggta tcaacaccat gcgttacaac
     1201 gaatccgaga tccgccgtat cggccatgtc gcctttggca tcgccatgaa gcggcgcaag
     1261 gatggacaaa aagcgaaagt ctgttccgtc gacaaggcca atgtgctgga aacgaccgaa
     1321 ctgtggcgcc aggtcatgat cgaagtggcc aaggaatacc cggaagtcga actcagccat
     1381 atgtatgtcg ataacgctgc catgcagttg gtgcgtgcgc ccaagcagtt cgacgtcatg
     1441 gtcaccggta atatctttgg cgatatcctg tcggatgagg cctccatgct gaccggctcc
     1501 atcggtatgt tgccgtcggc atcgctcgac gcgaacaaca aggggatgta tgagcccagc
     1561 cacggctctg caccggatat cgcgggcaag gatatcgcga acccgctggc gaccatcctt
     1621 tctgcagcca tgatgttgcg gtataccttc aacgacgaag cgaatgcctt gcgtatcgag
     1681 aatgcggtga agaaggcatt ggcggagggt taccgcacgg cggatatcca tacagacggt
     1741 tgcaggaaag taaagtgcag tgagatgggc gatgccgtgg tggccgcgct ttaatctctg
     1801 atcacaaaga acaagactcg ggaaatgatg atgttaaagg ttggtttcat aggctggcgc
     1861 ggtatggtgg gctcggtgct catgcaacgc atgcgtgagg agaacgactt cgccttgatc
     1921 gagccggtgt tcttcacgac ctcgcagcca ggcggcaagg ggccggatgt cggacgtgac
     1981 attccgccgt tgaaggatgc caagaacatc gctgaactga aggcgatgga cgtcatcatc
     2041 tcgtgccagg gtggcgacta taccaccgag atattcccgc aactgcgtaa ggccggctgg
     2101 aacggtcact ggatcgacgc ggcttccacg ctgcgcatgg aaaaggactc ggtcatcatc
     2161 ctcgatcccg tgaatcgcaa tgtgatcgac caggcgctcg
//
LOCUS       DATMTW010000211         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_421658, whole genome shotgun sequence.
ACCESSION   DATMTW010000211 DATMTW010000000
VERSION     DATMTW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421658"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VN084_04105"
     CDS             complement(<1..801)
                     /locus_tag="VN084_04105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWT28671.1"
                     /translation="MPYFITRLSLLLCLAFIGNAYAVDIPPAPSLAVKAYLLRDHNSG
                     NIVAQQGSNERVEPASLTKLMTAYVVFEAMKNGLLKPEQTLPVSVKAWKVEGSKMFIE
                     PNKPVTVDELLHGLIIQSGNDAAIALAEGVAVTEEAFVAMMNKDAARLGMLHTHYVNV
                     TGLPDPQHYTTAYDLALMASALIRDFPEEYKRFYGVKEYTYNKITQPNRNRLLWLDPF
                     VDGMKTGHTNSAGYCLIASAKRGDTRLISVVLGAPTENARASESQKLLN"
     gene            complement(852..1937)
                     /locus_tag="VN084_04110"
     CDS             complement(852..1937)
                     /locus_tag="VN084_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWT28672.1"
                     /translation="MKNLLLYSTLTLALLLVGCGGGTAVKPGPSAPAATTGDKPVTPP
                     KKGGGYYLDDGPGDNPPADIDAIPDAMPRAEPPLARANRPYSALGVRYTPMTEYQPYK
                     QRGIASWYGKRYHGQKTSSGEIYDMYGMTAAHTTLPLPSYARVTNPENGRSVIVRIND
                     RGPFHSDRLIDLSYAAAYKLRLIGKGSGLVEVEAIDPRGELFKPQTASGASTPQPIAS
                     SSTPAPLNVPPPIDPPTELQAPPPEAQPAQLAEASPNTATASTASTASTANTASTANS
                     AANAAAASPGNYVQVGAFKFKDNSDKLTEKLRQQDLAQNVPVESWYNAGTYRVRLGPY
                     SSRTDAERAAARIKQVLKVNAIVIDQK"
     gene            complement(1959..3053)
                     /gene="rodA"
                     /locus_tag="VN084_04115"
     CDS             complement(1959..3053)
                     /gene="rodA"
                     /locus_tag="VN084_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWT28673.1"
                     /translation="MISRLAKRLVSHIDSFLMGSLFFTLLVGLFVLYSASGQSLERVG
                     AQFANIVVALVVMWIAANIPPQHLERIALPLYILGLLLLLGVALFGEISHGARRWLHL
                     GVVRIQPSELMKIAVPMLLAWYFARREESTKFSDHVIGAVLLAIPVLFIMKQPDLGTS
                     LLIGASGFFILFLAGLNWRLLAGGAVLAGVLAPIFWNLLHDYQRKRIEILFDPYQDPL
                     GAGYHTIQASIALGSGGIAGKGWLNGTQAQLDFLPERTTDFIFAVFGEEFGLLGNLLL
                     LTLFTLLIGRGLVIAAQAQNTFCRLLAGSITLTFFTYVFVNIGMVSGILPVVGVPLPM
                     ISYGGTSLVTLMLGFGILMSIQTHKKLVTT"
     gene            complement(3046..>3595)
                     /locus_tag="VN084_04120"
     CDS             complement(3046..>3595)
                     /locus_tag="VN084_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWT28674.1"
                     /translation="GYTLVTPLQLAQATAIFANNGVAIQPRLVASIQQNSTGISRPTP
                     IVRSDQIPLKPENVELVKEGMIDVTRPGGTAASIGAGAPYAIAAKTGTAQVIGIKQNE
                     KYNEHAVNERHRDHALFIAYAPADDPKIALAVIVENGGHGGSAAGPIARKVMDYYLLG
                     KLPDHPKAVEAPIPPEEEEAHD"
BASE COUNT          647 a         1012 c         1207 g          729 t
ORIGIN      
        1 gttgagcagc ttctggctct cgctggcacg cgcgttctcc gtaggagcgc ccagcaccac
       61 cgagatcagg cgtgtgtcgc cgcgcttggc agacgcgatc aggcaatagc cggcgctgtt
      121 ggtgtgcccg gtcttcatgc cgtcgacgaa ggggtccagc cacagcaggc ggttacggtt
      181 gggctgcgtg atcttgttgt aggtgtattc cttgacgccg tagaagcgct tgtactcttc
      241 cgggaaatcg cggatcagcg ccgacgccat cagggcgaga tcatatgccg tggtgtagtg
      301 ctgtgggtct ggcaggccgg tgacattgac gtagtgggtg tgcagcatgc cgaggcgcgc
      361 cgcatccttg ttcatcatgg cgacgaaggc ttcttcggtc accgccacgc cctcagccag
      421 cgcgatcgcg gcatcgttac cggactggat gatcaagcca tgcagcagtt catccaccgt
      481 cactggtttg ttgggttcga tgaacatctt ggagccctcg actttccagg ccttgacgct
      541 caccggcagg gtctgctccg gtttcagcaa accattcttc atcgcctcga aaaccacgta
      601 ggccgtcatc agtttggtca gcgacgccgg ctcgacccgc tcgttgctgc cttgctgggc
      661 gacgatgttg ccgctgttgt ggtcgcgtaa caggtaggct ttgactgcca ggctgggtgc
      721 gggcgggata tcgacggcgt aggcgttgcc gatgaaggcg agacaaagca gcaggcttag
      781 gcgagtgatg aagtaaggca tagatctcga ttcgtactag atggtttcag gaaggtgcga
      841 taccaggcga tctatttctg atcgatgacg atggcgttca ctttcaacac ctgcttgatc
      901 ctggcggcag cgcgttcagc gtcggtgcga ctgctgtagg gcccgagtcg gacccgataa
      961 gttccagcat tataccaact ctcgacaggc acattttgcg ccaggtcttg ctggcgcaat
     1021 ttctccgtca gcttgtcgga gttgtccttg aacttgaaag cgccgacctg tacgtaattg
     1081 ccggggcttg ccgctgccgc gttcgcggca ctgttggcag tgctggcagt gttggcagtg
     1141 ctggcagtgc tggcagtgct ggcagtggca gtgttcgggc tggcttcggc cagctgcgcc
     1201 ggttgcgctt caggtggagg cgcctgcaac tcggtgggcg ggtcgatcgg tggcggtacg
     1261 ttcaagggcg cgggggttga actgctggcg atcggttgcg gcgtactcgc gccgctcgcg
     1321 gtctgcggct tgaacagttc gccgcgcgga tcgatggcct cgacctcgac cagcccgctc
     1381 cccttgccga tcaggcgcag cttgtaggct gcggcgtagg agagatcgat cagccggtcg
     1441 ctgtggaatg gaccgcggtc gttgatgcgc acgatcaccg agcgcccgtt ctccggattg
     1501 gtgacgcggg cataactggg caacggcagg gtggtatgcg ccgcggtcat gccgtacatg
     1561 tcgtagatct cgccgctgga ggtcttctgt ccgtgatagc gcttgccgta ccaggaggcg
     1621 atgccacgct gtttgtaggg ctggtactcg gtcatcggcg tatatcgcac acccagtgcg
     1681 ctgtagggcc ggttggcacg cgccagcggt ggttcggcgc ggggcatggc gtccgggatg
     1741 gcgtcgatat cggcaggcgg gttgtcgccc gggccgtcgt caaggtaata gccaccgccc
     1801 tttttcggcg gcgtcaccgg cttgtcgccg gtggtcgctg ccggagcgct gggtccgggt
     1861 ttgacggctg tgccgccacc gcagccgacg agtagtagtg ccagcgtaag ggtcgaatag
     1921 agcagaaggt ttttcatggt attcacgcgt tgatcctatc aggtcgtgac cagtttcttg
     1981 tgggtctgga tactcatcag gatgccgaat cccagcatca atgtcaccag cgaggtgccg
     2041 ccatagctga tcatgggtaa tggtacgccg acgacaggca ggatgccgct gaccataccg
     2101 atattgacga agacataggt gaagaaggtc agcgtgatgc tgccggccag caggcggcag
     2161 aacgtgttct gcgcctgcgc cgcgatcacc agcccacggc cgatcaacag tgtgaacagc
     2221 gtcaacagca aaaggtttcc gagcaggccg aattcttcac caaagaccgc gaagatgaaa
     2281 tcggtggtgc gctccggcag gaaatccagt tgcgcctggg tgccgttgag ccagcccttg
     2341 ccggcgatac cgccggagcc gagggcgatg ctggcctgga tggtgtggta accggccccc
     2401 agcggatcct ggtaggggtc gaagaggatc tcgatgcgct tgcgctggta atcgtgcagc
     2461 agattccaga agataggcgc cagcacgccg gccagtacgg cgccaccagc gaggagccgc
     2521 cagttgaggc ccgccaggaa gaggatgaag aagccggagg cgccgatcag cagcgaggta
     2581 ccgaggtcag gctgtttcat gatgaacagc accggtatcg ccagcagtac agcgccgatg
     2641 acatggtccg agaacttggt gctttcctcg cgccgcgcaa aataccaggc cagcaacatg
     2701 ggcaccgcga tcttcatgag ctccgagggc tggatgcgca cgaccccgag atgcagccag
     2761 cgtcgcgcgc catggctgat ctcgccgaac agtgcgacgc cgagcagcag caataggccg
     2821 aggatataga gcggcaacgc gatgcgttcc aggtgctggg gtgggatatt ggcggcgatc
     2881 cacatcacga cgagcgccac gacgatattg gcgaactgtg cgccgacgcg ttcgaggctc
     2941 tgccctgagg cgctatacag cacgaacagg ccgaccagca gggtaaagaa caggctgccc
     3001 atgagaaatg aatcgatatg ggacaccagg cgcttcgcca gtcggctaat catgggcttc
     3061 ctcctcttcc ggcggaatgg gcgcttcgac ggccttggga tggtccggta gtttgcccag
     3121 caaatagtaa tccatcacct tgcgtgcgat ggggccggcg gcagaaccgc catggccacc
     3181 attctcgacg atgacggcga gcgcgatctt ggggtcgtcg gctggcgcgt aggcgatgaa
     3241 tagcgcatgg tcgcgatgcc gctcattgac ggcgtgttca ttgtacttct cgttctgctt
     3301 gatgccgatg acctgcgccg taccggtctt ggctgcgatc gcataaggag cgccagcgcc
     3361 gatactggcg gcagtgccgc caggccgggt gacgtcgatc atgccttctt tcacaagctc
     3421 gacgttctca ggcttgagcg ggatctggtc ggaacggacg atgggtgtcg gccggctgat
     3481 gccggtactg ttctgctgga tgctggcgac cagccgaggc tggatggcga cgccattgtt
     3541 ggcaaagatc gcggtagcct gggctagctg caatggcgtg accagggtat agccc
//
LOCUS       DATMTW010000212         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_657227, whole genome shotgun sequence.
ACCESSION   DATMTW010000212 DATMTW010000000
VERSION     DATMTW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657227"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VN084_04125"
     CDS             <1..1286
                     /locus_tag="VN084_04125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWT28675.1"
                     /translation="DEAYNIGSDPVLGYLNAHNIGNLAVASGCDALHPGYGFLSENPE
                     LAEICKRRGIKFIGPDAPVIRQMGDKIQARNAMIKARIPCVPGSEGNLENVEEARKLA
                     NKIGYPVMLKATNGGGGRGIRRCNDEKELLSNYDRVISEASKAFGKPEVFLEKCVVNP
                     RHIEVQVMADSHGNVIHLFERDCSIQRRNQKLIEIAPSPQLTNAQREYIGNLAVKAAK
                     AVGYKNAGTVEFLLDSDNNFYFMEMNTRLQVEHTVTETITGIDIVQEQIRVADGLPLQ
                     YKQKDVHYRGFAMEFRINAEDPKNDFLPSFGKITRYYAPGGPGVRMDAAMYSGYVIPP
                     YYDSMCAKLTVWALNWESVIERGRRALDDMVVYGVKTTIPYYQEILKHSAFRAAHFDT
                     SFVETHPELINYATEFPPELIAAAISAAIAAHEGI"
     gene            1320..>3035
                     /gene="oadA"
                     /locus_tag="VN084_04130"
     CDS             1320..>3035
                     /gene="oadA"
                     /locus_tag="VN084_04130"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HWT28676.1"
                     /translation="MAKVHVSELVLRDGHQSLIATRMRTEDMLPICPKLDAIGFWSLE
                     AWGGATFDACVRYLKEDPWERLKKLRKALPNTRIQMLLRGQNLLGYRHYSDDVVRAFV
                     QKSADNGVDVFRIFDAMNDARNLRVSIEAVKKSGKHAEGTISYTTSPVHDIAYFVALA
                     KEMEGMGADTLAIKDMAGLLTPQVTYDLVKALHAAIKLPIHLHSHATSGLSAMCLLKG
                     VEAGAAIIDTSNSSFGEGASHPSTESLVAALQGTEYDTGLDLAALQEITAYFREVRKK
                     YWQFESDFTGVDTRVLVNQVPGGMISNLSNQLKEQGALGRMDEVLAEIPRVREDLGFP
                     PLVTPTSQIVGTQAVLNVMTGARYKSVTNEVKNYLLGHYGKAPAAVNTDVRHLAVGDA
                     EVITCRPADLLEDEMDKLRAESENLAKSEEDVLTYAMFPDLAKTFLQERNAGSLKPEP
                     LLTKEAVTSSGAHYAPNEFKVTLHGETFHIKLTGSGHQGEEQRPFYVSVDGIAEEVIV
                     ETLSEIEVSGGKSSGNGKKKAPSTSSSGRPRPSHSGCVTAAMPGTIGDVKVKAGDKVN
                     AGDAVL"
BASE COUNT          700 a          895 c          857 g          583 t
ORIGIN      
        1 ctgacgaggc ctataacatc ggctcagacc ccgtgctggg ctacttgaat gcgcacaata
       61 tcggcaacct tgctgtcgca tcaggctgcg atgccttgca tcccgggtac ggtttcctgt
      121 cggagaatcc ggagcttgcc gagatctgta aacgtcgcgg catcaagttc atcggtccag
      181 atgcgccagt gatccgccag atgggcgaca agatccaggc ccgcaatgcc atgatcaagg
      241 cgcgcatccc ctgcgtgcct ggcagcgagg gcaatcttga gaatgtcgaa gaagcccgta
      301 agcttgccaa taagatcggc tatcccgtca tgctcaaggc aaccaatggt ggcggtgggc
      361 gcggcatccg ccgctgcaac gatgagaaag agctgctgag caattatgat cgcgtcatct
      421 ccgaggcgag caaggcgttc ggcaagcccg aggtattcct tgagaagtgc gtggtcaacc
      481 cgcgtcacat cgaagtgcag gtcatggccg atagccacgg caacgtcatt catttgtttg
      541 agcgcgactg ctctatccag cgtcgcaacc agaagctgat tgagatcgcc ccttcgccac
      601 agctcaccaa tgcccagcgc gagtacatcg gcaacctggc agtcaaggct gccaaggcgg
      661 tgggctacaa gaacgctggc acggtcgaat tcctgctgga ttcggacaac aacttctact
      721 tcatggaaat gaacactcgt ctgcaagtcg agcatacggt gacggaaacc atcaccggga
      781 tcgacatcgt gcaggagcag attcgcgtgg ccgacggcct gccgctgcag tacaagcaga
      841 aggacgtaca ttaccgtggc tttgccatgg agttccgcat caatgccgaa gatccgaaga
      901 acgactttct gcccagtttc ggcaagatca cgcgttacta cgcgcctggc ggacctggcg
      961 ttcgcatgga cgcagccatg tacagcggct atgtgatccc gccctactac gactcgatgt
     1021 gcgccaagct gacggtctgg gcactgaact gggaaagcgt gatcgaacgc ggtcgacgcg
     1081 cactggatga catggtggtc tacggcgtaa agacgacgat cccctactac caggagatcc
     1141 tgaagcattc ggccttcagg gccgcacatt tcgacacgag cttcgtggaa acccaccccg
     1201 agctgatcaa ttacgctacc gagttcccac ccgaactcat cgctgccgcg atctctgccg
     1261 cgattgcggc acatgaaggc atttgagcaa gaattaatct aaggtgagaa gttaacaata
     1321 tggcaaaagt acatgtttct gaactagttt taagggatgg acatcaatcc ctcatcgcaa
     1381 cccgcatgcg cacggaagat atgttgccta tctgtccgaa gctggatgcg atcggtttct
     1441 ggtcccttga agcctggggc ggtgccactt ttgacgcctg tgtacgctat cttaaggaag
     1501 atccttggga gcgcctgaaa aagttacgca aggccctgcc caacacccgc atccagatgc
     1561 tgctgcgcgg acagaacctg ttgggctatc gccactattc tgacgatgtc gtacgcgcct
     1621 tcgtgcaaaa atccgcggat aacggcgtcg atgtgttccg tatcttcgat gccatgaacg
     1681 atgcgcgtaa tcttcgcgtc tctatcgaag cggtgaagaa atccggcaag cacgcggaag
     1741 gcacgatcag ttacaccacc agccctgtgc acgacatcgc ctatttcgtc gcattggcca
     1801 aagagatgga aggtatgggc gctgacaccc ttgccatcaa ggacatggcg ggcctactta
     1861 cgcctcaagt cacctatgac ttggtcaaag cactccacgc agccatcaag cttcctatcc
     1921 atcttcacag ccatgcgact tccggtctct ctgcgatgtg cctgctgaaa ggcgttgaag
     1981 ctggtgcagc catcatcgat acgagcaact cctcgtttgg cgaaggcgcc agccacccct
     2041 ccaccgagag tctggtagcc gcgctgcaag ggaccgaata cgacaccggc ctcgatctcg
     2101 cagcactaca agagatcact gcctacttcc gtgaagttcg caagaaatac tggcagttcg
     2161 aaagtgattt caccggggtc gatacccgtg tgctggtcaa ccaggttccg ggcggcatga
     2221 tctccaacct gtccaaccag ctgaaagagc aaggcgcttt gggtcgcatg gatgaggtgc
     2281 tggctgagat cccgcgggta cgtgaagacc tgggtttccc accgcttgtc acgccgacct
     2341 cgcagatcgt gggcacgcag gccgtgctca acgtcatgac tggcgcgcgc tacaaatcgg
     2401 tgaccaacga agtcaagaac tatctccttg gccactatgg caaggcacca gccgcggtca
     2461 acaccgatgt ccggcatctg gccgtgggcg atgccgaagt catcacctgc cgacctgccg
     2521 acctgctcga agacgagatg gacaagctgc gcgccgaatc cgaaaacctg gcgaagtccg
     2581 aggaagacgt gctgacctat gccatgttcc ctgacttggc caaaaccttc ctgcaagagc
     2641 gtaatgccgg cagtctgaag cccgaaccgc tactgaccaa ggaggccgtg acgtcgagcg
     2701 gcgcgcatta cgcgccgaac gagttcaagg tcacgctgca cggggagaca ttccacatca
     2761 agctcaccgg cagcggccat caaggtgagg aacagcgtcc gttctacgtc tcggtcgatg
     2821 gtatcgccga ggaagtgatc gtcgagacct tgagcgagat cgaagtatcg ggcggcaaga
     2881 gcagcggcaa cggcaaaaag aaggctccca gcaccagcag cagtggtcgc cctcgcccgt
     2941 cgcacagcgg ttgcgtaacg gctgcgatgc cgggcaccat cggcgacgtc aaggtcaagg
     3001 cgggcgacaa ggtcaatgcc ggcgatgccg tgctc
//
LOCUS       DATMTW010000213         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_489166, whole genome shotgun sequence.
ACCESSION   DATMTW010000213 DATMTW010000000
VERSION     DATMTW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489166"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1116
                     /locus_tag="VN084_04135"
     CDS             100..1116
                     /locus_tag="VN084_04135"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWT28677.1"
                     /translation="MDIYSFFKPLLFKLDAEKAHDLTLKSLRFAAKAGLLRLLGKPPV
                     TRPRQVMGLTFPSAVGLAAGLDKNAACIDGMAALGFGFIEVGTVTPRPQPGNPKPRLF
                     RVTEADGIINRFGFNNLGVDQLIENVKRTKYKGILGINIGKNFDTPNARAVDDYLICL
                     RKVYPHASYVTVNISSPNTKNLRALQEKEALGNLLAALKKEQATLAEAYGRYVPIALK
                     IAPDLEAEQVHEIADLLMQHQFDGVIATNTTLSRVAVEGMLHAQETGGLSGAPVRERS
                     TLVIRQLADRLQGRVPIIGVGGIMNGQDAAEKIAAGASLVQIYSGLIYRGPHLVKEVA
                     RTLG"
     gene            1128..1337
                     /locus_tag="VN084_04140"
     CDS             1128..1337
                     /locus_tag="VN084_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HWT28678.1"
                     /translation="METNVGTVDRVLRVIISLALLSLVFLIDSHLRWIGMIGLVTLFT
                     AVTSWCPAYAMLGIKTRRTPPPPQE"
     gene            1338..>1791
                     /locus_tag="VN084_04145"
     CDS             1338..>1791
                     /locus_tag="VN084_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWT28679.1"
                     /translation="MPQPILYTYRRCPYAMRARMALHYSGIEVEMREIALKAKPTAML
                     AASPKGTVPVLVLPDGTVIDESLDIMHWALARHDPHDWLSDAVQSLTLIAENDGAFKQ
                     ALDRYKYAVRFPEHAPEFYRVEGERFLALLEARLQQHACLLDDAVRLAD"
BASE COUNT          363 a          523 c          539 g          366 t
ORIGIN      
        1 gaagccggaa tccagtggct tgttttcaaa ccctcacgca tccccagccg tgtgaaaact
       61 acaagcaagc gcgattgcta aatcaagtaa aatccgggta tggatatcta ctcatttttc
      121 aagcccttgc tgttcaagct cgatgccgaa aaggcacacg atctcaccct caaatccctg
      181 cgtttcgctg ccaaagccgg cctattgcgc ctgctgggca agccgcctgt gacgcgtccg
      241 cggcaggtga tggggctgac attccccagt gcggtcggtc ttgctgccgg gctggacaag
      301 aatgcggctt gtatcgacgg tatggccgcg ttgggtttcg gtttcatcga ggtcggcacc
      361 gtaacgccgc gcccgcagcc cggtaacccc aagccgcgtc tgtttcgcgt gacggaggcg
      421 gatggcatca tcaatcgctt cgggttcaac aatttaggtg tggatcagct gatcgagaac
      481 gtgaaacgca cgaaatacaa aggcatcctc ggcatcaata tcggcaagaa tttcgatacg
      541 ccgaacgcgc gggcggtgga cgattacctg atctgcctgc gcaaggttta cccacatgcg
      601 agttatgtca cggtgaatat ctcgtcgccc aacaccaaga acctgcgcgc tttgcaggag
      661 aaagaggcgc tgggtaatct gctggcggcc ttgaagaagg aacaggcgac gctcgccgag
      721 gcttatggcc gttatgtgcc gatcgcgctc aagatcgccc cagatctcga agccgagcaa
      781 gtgcacgaga tcgcggatct cttgatgcag catcagttcg atggcgtcat cgccaccaac
      841 accacgctgt cgcgcgtcgc ggttgagggg atgctccatg cccaggaaac tggcgggctt
      901 tccggcgcgc cggtgcgcga gcgttcgacg ctggtgatcc gtcagctggc ggatcgtctg
      961 caggggcgcg tgccgatcat cggcgtcggc ggtatcatga acgggcagga tgccgccgag
     1021 aagatcgcgg ccggcgccag cctggtgcag atctatagcg ggttgatcta tcgtggcccg
     1081 catcttgtca aagaggtagc tcgtacgctt ggataaggag gtgtgacatg gaaaccaatg
     1141 tcggtactgt tgaccgtgtt ctacgtgtga tcattagcct ggcgttgctc agcctcgtct
     1201 ttctgatcga ctcccacctg cgctggatag gcatgatcgg tctcgtgacg ctgttcacgg
     1261 ccgtcactag ctggtgcccg gcctatgcca tgctggggat caagacccgc cggactccgc
     1321 caccgccaca ggaatagatg ccgcagccca tcctctatac ctatcggcgt tgtccatacg
     1381 ccatgcgtgc ccgcatggcc ctgcattaca gcggtatcga agtcgagatg cgcgagatcg
     1441 cgctcaaggc aaagcccaca gcgatgcttg cggcatcgcc caagggcacg gtgccggtcc
     1501 tggtgttgcc ggatggcacg gtgatcgacg agagcctgga catcatgcac tgggcgctcg
     1561 cccggcatga cccacatgac tggttaagcg atgccgtgca aagtctgacg ctgatcgccg
     1621 agaacgacgg cgcgttcaag caggcgctgg atcgatacaa atatgcggtg cgattcccgg
     1681 aacatgcgcc agaattctat cgtgtcgaag gtgagcgctt ccttgccctg ctcgaggcgc
     1741 gccttcaaca acatgcctgc ctgctggatg acgcagtgcg gctagccgat a
//
LOCUS       DATMTW010000214         5218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_120867, whole genome shotgun sequence.
ACCESSION   DATMTW010000214 DATMTW010000000
VERSION     DATMTW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120867"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN084_04150"
     CDS             <1..181
                     /locus_tag="VN084_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28680.1"
                     /translation="KALTSLLLLILLSACSPSQDQTPKIADDQRKVLEDAKSLDATMQ
                     QSTEEQARQTEEQTR"
     gene            198..629
                     /locus_tag="VN084_04155"
     CDS             198..629
                     /locus_tag="VN084_04155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWT28681.1"
                     /translation="MSACLVIRAGWSAHETALRAIREAVFIREQAVPVALEWDGQDEH
                     AQHLLACDDRGRAIGCARILAGGVIGRMAVLPAWRRRGIGAALLAAAVAYCREQRWSP
                     ICLSAQTHAIGFYARAGFVVCSESYLDAGIPHRDMRLDLSA"
     gene            699..1250
                     /gene="fae"
                     /locus_tag="VN084_04160"
     CDS             699..1250
                     /gene="fae"
                     /locus_tag="VN084_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HWT28682.1"
                     /translation="MSKIIFKAGEATIFSEGKDVTAAMPEILIGAVDGPVGTAFANMM
                     AQSKGHTAMFAVRDINQLVRPATMMVPKVTLKGSTDVGLFGGVVQAATADAVVDAVIE
                     GIIPKDQVNDLCIISLVWIDPGCIALEKEGKLDKADMYKNNYEATKLALKRALSDEPS
                     IDELIKNRHTIKHCMWEESWGEI"
     gene            1418..1771
                     /locus_tag="VN084_04165"
     CDS             1418..1771
                     /locus_tag="VN084_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWT28683.1"
                     /translation="MHEFIDYLHELFEQLGPITTRRMFNGHTLYHQGLPIGIVYDETL
                     YLKADGEHAEACTAQGLPQFTYEQRGKRIGLPYYQAPESILEDRAEAAIWGRKAFEAA
                     LRMQARSSRRKHGKD"
     gene            complement(1777..3054)
                     /locus_tag="VN084_04170"
     CDS             complement(1777..3054)
                     /locus_tag="VN084_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWT28684.1"
                     /translation="MTARQVLVVDDEIGIRELLRDILQDEGYHVRLAENAAEAREWRH
                     QARPDLVLLDIWMPDCDGISLLKEWGNGGLLTMPVVMMSGHGTIDTAVEATRIGAFDF
                     LEKPIALQKLLKTVNAALKHGEQLPRSDMSLIGLGKSQVINELRQRLEQIAQLRSPLL
                     LIGGKGSGVELCARFLHEPETPWLELKEHHILAEAPLDLLEQVRDGCVFIAEVAELNK
                     TEQKGLQLLLGKAEKFGLRVICATACALPQLVEEGQFDSQLLQTLSITTLRVPGLDEH
                     REDIPDLARAMVALLVESSEATYREFDIAALNALRNADWPGNLAQLDSVIRNLMQTSL
                     GDKITLDDVKRVISQFESIAQAMPLQAPASAKGLALDLDTPLREARDAFERLYFEHHI
                     AKAGGNMSKVAEYVQLERTHLYRKLKQLGMKTK"
     gene            complement(3058..5205)
                     /locus_tag="VN084_04175"
     CDS             complement(3058..5205)
                     /locus_tag="VN084_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28685.1"
                     /translation="MRYIVFISAAIGAVLLYLLSHASANTAASGTHYTILLGLNIALA
                     VVLMVLIGVQLWRLYRQMKARVIGSRLTLRLLGAFALMAIIPGVIVYAVSVNFLTRSI
                     ESWFNVKVEAALDGGLRLGQSALDIMLADVEEKGESMALGLAFQPSSMHLALLNDLRE
                     KSGIQEAVLLTAQGRILAFSSNDPTSFLPELPSISQLRQARQRVYGIIEPIPGKGLYL
                     RALAPVSVSELGGETRILQLLQPVPKSLSATAESVQDVYQDYQELSFSRDSLKDVFAL
                     TLTLVLMLAMLTAVAIAFVLSRRLSAPLGTLAEGTRAIARGDYSTMLPAHGKDELGIL
                     VQSFNSMTRQLSEATQAAERNRARVEAARSYLETILAHLSSGVLAFNAEAHLRTYNMA
                     ARNILGLTLDDAVDQPLARLAESQPALEPFTQAILAHFYGEEKGEWQEQMEIPTAQGR
                     QILLVRGTRLLDSPESGYVVVFDDITTMVQAQRDAAWGEVARRLAHEIKNPLTPIQLS
                     AERLEHKLTPKLAGTDVTMLKRATDTIVSQVTAMKAMVNEFSEYARAPALNLGNIDLN
                     QLIRDVLALYEPVGVAIEVALAERMPTITGDITMMRQVLHNLLQNAQDALDGQQSPTI
                     WICSSLEGDKVKLTVRDSGVGFPVEIISRAFEPYMTTKRHGTGLGLAIVKKIVEEHRG
                     TIRLENADEGGAVVTVTLPVTPGASNQAKQDGA"
BASE COUNT         1042 a         1579 c         1541 g         1056 t
ORIGIN      
        1 gaaagccttg accagcctac tcttgctcat cttgctcagc gcatgttcgc cgagccagga
       61 ccagaccccg aaaatcgcgg atgatcagcg taaggtgctg gaagatgcga aatccctgga
      121 tgccaccatg cagcagtcta cagaggagca agcacggcag accgaggagc agacccgttg
      181 atccagagcc gttgattttg tccgcttgcc ttgtcatccg tgccggctgg tctgcgcacg
      241 agaccgcctt gcgggcgatc cgcgaagctg tgttcatccg tgaacaggcc gtgccggtgg
      301 cgcttgaatg ggatggtcag gacgagcatg cgcagcacct gctggcatgc gacgatcgtg
      361 ggcgggccat tggttgcgca cgtatcctcg caggcggcgt catcggacgt atggcggtat
      421 tgcccgcctg gcgccggcgc ggcatcggcg ctgccctgct ggccgctgcc gtcgcctact
      481 gtcgcgagca gcgctggtcg ccgatatgcc tgtcggccca gacgcatgcg atcgggttct
      541 atgcgcgtgc aggcttcgtc gtctgtagtg agtcctatct cgacgccggc attccccacc
      601 gggacatgcg acttgaccta agcgcttgat ttgccgatgc ctagcggctt gggtaaaatg
      661 ccgggtttcc cttccagaca atcaaaaagg aacgcgcaat gtccaaaatc atattcaagg
      721 ctggcgaagc caccattttc agtgaaggta aagacgtcac tgcggcgatg ccggagatcc
      781 tgatcggcgc ggtcgacggt ccggtcggca cggctttcgc caatatgatg gcacagagca
      841 agggccacac cgccatgttc gccgtgcgtg acatcaacca actcgtgcgc cctgccacca
      901 tgatggtgcc taaagtcacg ctcaagggca gcaccgatgt cggcctgttc ggtggcgtgg
      961 tgcaggcagc gaccgccgat gcggtggtcg acgctgtcat cgaaggcatc atccccaagg
     1021 atcaggtcaa cgatctttgt atcatcagcc tagtgtggat cgatccaggt tgtatcgcgc
     1081 tggaaaagga aggcaagctg gacaaggccg atatgtacaa gaacaactac gaagccacca
     1141 agcttgcact caagcgcgca ttgagcgacg aaccctcgat cgatgagctg atcaagaatc
     1201 gccacaccat caagcactgc atgtgggaag aatcctgggg cgagatctaa gccgagtgac
     1261 attaagaagg ccagctgtca ctactgactg gtcgcaataa aaaaggcagc gcaagctgcc
     1321 ttttttattg ccggctgatc atcctggtct gtattgtcat ggcctgggat caacgaaagg
     1381 ttttccatgc atcttctttc cgccgggccg aactggcatg catgagttca tcgattacct
     1441 gcatgaactc tttgaacaac tggggcccat caccacacgc cgcatgttca acggccatac
     1501 gctctaccat cagggcctgc ccatcggcat cgtctacgac gagaccttgt acctgaaagc
     1561 ggacggcgaa catgccgagg cctgcacggc gcaaggcttg ccgcagttca cctacgagca
     1621 gcgcggaaag cgcattggcc taccttatta ccaggcgcca gaatcgattc ttgaggatag
     1681 ggcagaggca gcgatatggg ggcgtaaggc ctttgaggca gccttgcgca tgcaggccag
     1741 aagctcgcgt cgcaagcacg gcaaggactg agccgcttat ttggtcttca tgcccaactg
     1801 tttgagctta cggtaaagat gggtgcgttc gagttgcacg tactccgcga ctttgctcat
     1861 gttaccgccg gccttggcga tatgatgttc gaaatacagc cgctcgaacg catcacgggc
     1921 ttcgcgtagc ggggtgtcga gatcaagcgc cagacctttc gcactcgcgg gcgcctggag
     1981 cggcatcgcc tgtgcgatcg actcgaactg gctgatgacg cgcttgacgt catcgagcgt
     2041 gatcttgtcg ccaagactgg tctgcatcag gttgcggatc acgctgtcga gctgcgcgag
     2101 attgccgggc caatcggcgt tacgcaatgc gttgagtgcg gcgatatcga attcgcgata
     2161 ggtcgcttcg gaggattcca cgagcaaggc caccatggcc cgcgccaaat cagggatatc
     2221 ttcccggtgc tcatcgaggc cggggacgcg caaagtcgtg atcgagagcg tttgtagcag
     2281 ctgactgtcg aactggccct cctcgaccag ttgcggcagt gcacaagcag tcgcgcaaat
     2341 gacgcgcagg ccgaactttt ccgccttgcc cagtagtaac tgcagcccct tttgctcggt
     2401 tttgttgagt tcggctactt ccgcgatgaa cacgcaaccg tcccgcacct gctccagtag
     2461 atccagcggt gcttcggcca gaatatgatg ctctttgagt tcaagccagg gcgtctccgg
     2521 ctcgtgcagg aaccgcgcac atagttcgac accactgccc ttgccgccga tcagcaacaa
     2581 aggtgatctg agctgagcga tctgctccag gcgttgacgc aattcattga tgacctggct
     2641 cttgccgagg ccgatcaggc tcatatccga ccgcggtaac tgttcgccgt gcttcagggc
     2701 cgcgttgacg gtcttcagca gcttttgcag ggcgatcggc ttttcgagga agtcaaaggc
     2761 gccgatgcgg gtcgcctcga cggcagtatc gatggtgccg tgacccgaca tcatgaccac
     2821 cggcatggtc agcagtccgc cgttgcccca ctccttgagc aggctgatgc cgtcgcagtc
     2881 gggcatccag atgtccagca agaccaggtc aggacgcgcc tgatgccgcc attcacgcgc
     2941 ctcggcggca ttctccgcca accggacgtg atagccctcg tcctgcagaa tgtctctcaa
     3001 cagctcgcgg atgccgattt cgtcatctac gaccagtacc tgccttgccg tcattcgcta
     3061 cgctccatcc tgtttcgctt gattggatgc cccgggtgtg accggtagag tcaccgtcac
     3121 caccgcaccg ccttcgtccg cgttctccag acgaatggtc cccctgtgct cttcgacgat
     3181 cttcttgacg atggccaagc cgagcccggt gccatgccgc ttggttgtca tatagggttc
     3241 aaaggcacgg gagatgatct ctacagggaa acccacgccg ctatcgcgta cggtcaattt
     3301 taccttatcg ccctcaaggc tgctgcatat ccagatcgtc gggctctgct gtccatccag
     3361 cgcgtcctgc gcattctgca gcagattgtg caagacctgc cgcatcatgg tgatgtcgcc
     3421 ggtgatggtc ggcatgcgct cggcgagcgc gacttcgatc gcaaccccga ccggctcgta
     3481 caaggccagt acgtcgcgga tcaactggtt aaggtcgata ttgccaaggt tcaatgccgg
     3541 cgcccgcgca tactcgctga actcgttgac catcgctttc atcgcggtca cctggctgac
     3601 gatggtgtca gtcgcgcgct tcagcatggt cacatcggtc cccgccagtt tgggcgtgag
     3661 tttatgttcc agacgctcgg ccgagagctg gatcggcgtc agcggattct tgatttcatg
     3721 ggccagtcgt cgcgccactt cgccccaggc cgcatcccgc tgggcttgca ccatggtggt
     3781 gatgtcgtca aagacgacca catagccact ttccgggcta tccagcaggc gcgtgccgcg
     3841 taccagcagg atctgcctgc cctgcgcggt cgggatctcc atctgttcct gccactcgcc
     3901 tttctcctca ccgtaaaaat gagccaatat ggcctgggtg aaaggctcca gcgcaggctg
     3961 cgattccgcc agtcgggcca ggggttggtc caccgcgtcg tcaagcgtca gtccaaggat
     4021 atttcgggcg gccatgttgt aggtacgcag gtgcgcttcg gcattgaacg ccagcacgcc
     4081 cgaagacaag tgcgccagga tggtctcgag ataactgcgc gccgcctcga cacgagcacg
     4141 gttacgttcc gctgcctggg tggcttccga gagctgccgc gtcatgctgt tgaaggattg
     4201 cacgaggata cccagttcat ccttgccatg ggccggtaac atcgtgctgt aatcaccacg
     4261 cgcgatcgcg cgcgtacctt cggcgagtgt acccagcggc gccgacaagc ggcgactgag
     4321 cacgaacgcg atggccactg ccgtcagcat ggccagcatc agcaccagcg tgagcgtcag
     4381 cgcgaacacg tccttcagcg aatcccggct gaatgaaagc tcctggtaat cctgatagac
     4441 atcctgcacc gactctgcgg tggctgacag cgatttgggc accggctgca acaattgcag
     4501 gatgcgcgtc tcgccgccaa gctcggagac ggaaacaggg gccagcgccc gtagatacaa
     4561 ccccttgcca ggaatgggct cgatgatgcc atagacgcgc tgtcgtgcct ggcgcagctg
     4621 cgagatgctg ggcaactcgg gcaagaagct ggtcggatcg ttgctggaaa aggccaggat
     4681 gcggccctgg gccgtcagca gcacggcttc ctggataccg ctcttctcgc gcagatcgtt
     4741 aaggagcgcc agatgcatgg acgatggctg gaatgccagc ccgagggcca tgctctctcc
     4801 tttctcctcg acatccgcca gcatgatgtc gagcgcgctc tgaccgaggc gcaagccccc
     4861 atcgagcgca gcttcgacct tgacgttgaa ccaggattcg atcgagcgcg tgaggaaatt
     4921 caccgatacc gcatagacga tgaccccggg aatgatggcc atcagcgcga acgcgccaag
     4981 caggcgcaag gtcaaccggc tgccgatgac gcgggccttc atctgtcggt agagccgcca
     5041 cagctgcacc ccgatcagta ccatcaacac cacggccagc gcgatgttca atccaagcag
     5101 gatggtgtag tgggtgccgg acgccgcagt gttggcgctg gcatgcgata acaggtagag
     5161 aagcactgcg ccgatggcgg cgctgatgaa aacgatatat ctcatcggtt gaagtcta
//
LOCUS       DATMTW010000215         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_489232, whole genome shotgun sequence.
ACCESSION   DATMTW010000215 DATMTW010000000
VERSION     DATMTW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489232"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..981
                     /locus_tag="VN084_04180"
     CDS             151..981
                     /locus_tag="VN084_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HWT28686.1"
                     /translation="MQQIPFRSPRAIRGQALTELAVMAALLVPMFLLIPIVAKYGHMN
                     QMAQQAARNAAWEASVSRDYAVANPREAQRKALDRNFASADARIRSDVGSVAMRGEFG
                     DQMLNTFSGRKLLERDNLRVPSMGNTESPGYMDEALVPIETVTAPFGKFTLNKQGYVT
                     AHVKLNIRDLKLADGGRARYLEPLDNLNLVLDRKQTLLVDAWNASGPRPGNGSRNVVD
                     TVRPLAPTSKLKGVDKLTRMIKPPLDKLPVFGAIGGLKLGTIEPDIVPADKLAEYPAR
                     "
     gene            1005..1661
                     /locus_tag="VN084_04185"
     CDS             1005..1661
                     /locus_tag="VN084_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28687.1"
                     /translation="MAFASLALVVGPAIAADWPDVPVPEGARGEIVSSDMLYNGLHMR
                     ASRFTVKMPVDKVKAFYREEWGGRMIDTPHRGKSVLGYPTKDAKHYVTVELSQAGNAT
                     QGQIGVMELPKQPPPAGSVGAGFARMPDTTVAEDIVYLDTPARVRTISMVNRYSPQQN
                     SDFYARNLAPKGYVREPSKTPCKATSAECIARYVNGDKRVTVSSSKSSAGTVIIAVIE
                     "
     gene            1658..1870
                     /locus_tag="VN084_04190"
     CDS             1658..1870
                     /locus_tag="VN084_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28688.1"
                     /translation="MNRINANGFRFSGPGRQTGQATIEYTIVVMLVVIVLVARPSVIE
                     EVVTAMKEAYSAFVYAISWSDIPLRN"
BASE COUNT          436 a          624 c          630 g          333 t
ORIGIN      
        1 gatggcctat ttgaatccga tgcctctgga gagcaagcag ggctcgcaag gggtgggctg
       61 caatctgcaa ctgcaatcga tgacagtcag caaaggcctg ctgccggaag taacccgtgc
      121 ccttgctgcc acgcaaaagg aagaatgagc atgcagcaga ttcctttccg gtcaccacgc
      181 gcaattcgcg ggcaggcgct gacagaactg gcggtgatgg cggccctgtt ggtgccgatg
      241 ttcctgctga tccccatcgt ggccaagtat ggccacatga accagatggc ccagcaggcg
      301 gcacgcaatg ccgcatggga agcgtcggtg tccagggact acgcggttgc caacccccgt
      361 gaggctcaac gcaaggcgct ggatcgtaat ttcgcttccg ccgatgcaag gatccgcagc
      421 gatgtcggta gcgtcgccat gcgcggcgag ttcggcgacc agatgctgaa cacgttctca
      481 ggccgcaagc tgctggagcg cgacaacttg cgggtcccga gcatgggcaa tacggagtcg
      541 cccggctaca tggacgaggc cctcgttccc atcgaaacgg ttacggctcc cttcggaaaa
      601 ttcacgttga acaagcaggg ctatgtcacc gcccacgtca agttgaacat ccgtgatctg
      661 aagctcgcgg atggcgggcg agcgcgttac ctggagccac tcgacaacct gaatctcgtg
      721 ctagaccgca agcagacctt gctggtcgat gcatggaatg cgtccggtcc ccgtcccggc
      781 aacggcagtc gcaacgtggt ggatacggtc aggccgctgg cacccacgtc caagctgaag
      841 ggcgtggaca agctgacaag gatgatcaag ccgccgttgg acaagctgcc ggtgttcggc
      901 gcgattggtg gcctgaagct tggcaccatc gagccggaca tcgtcccggc cgacaaactg
      961 gcggagtacc cggccagatg atgagccgcc actcctttcc agtcatggcg ttcgcatcgc
     1021 ttgcattggt ggtcggcccg gcgatcgcgg cggattggcc ggacgtgccc gtgccggaag
     1081 gtgccagggg cgaaatcgtc agcagcgaca tgctctacaa cggcctgcac atgcgtgcca
     1141 gccgcttcac cgtgaagatg ccggtcgaca aggtcaaggc gttctaccga gaggaatggg
     1201 gcgggcgcat gatcgacacg ccgcatcgcg gcaagagtgt gctcggctat ccgaccaagg
     1261 atgcgaaaca ttatgtcacc gtcgagctga gccaagccgg aaatgccacc caaggccaga
     1321 taggggtgat ggaactgccg aagcaaccac cgcctgccgg cagcgtcggc gcaggcttcg
     1381 cgcgcatgcc cgacaccacg gtggccgagg acatcgtcta cctggacacg ccggcgcgcg
     1441 tacggaccat cagcatggtc aatcgctaca gcccgcagca gaacagcgac ttctacgcgc
     1501 ggaatctggc gccgaaaggc tacgtcaggg aacccagcaa gacgccctgc aaggccacca
     1561 gtgcggagtg catcgcccgc tacgtcaacg gcgacaagcg ggtcacggtt tcgtcgagca
     1621 agtcgtcggc gggaaccgtc atcattgcgg tgatcgaatg aaccggatca atgcgaacgg
     1681 attccgcttc agcgggcccg gccggcaaac gggccaggcc accatcgaat acaccatcgt
     1741 ggtgatgctg gtggtgatcg tgctggtggc caggccaagc gtcatcgaag aggtcgtgac
     1801 cgcaatgaag gaagcgtact cggcattcgt ctacgccatt tcctggtccg acatcccgct
     1861 ccgcaactaa cgtccagcag ccgaaagcaa acgccacccc atgcagaagc cgaacatcag
     1921 caggaacctg ctctacatca tcatcgccgt ggtcatggcg atcctcgccg cattgatcgc
     1981 ggtgcgctac gtgcagaaac aggtcgacga acgcacccgc gac
//
LOCUS       DATMTW010000216         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_355691, whole genome shotgun sequence.
ACCESSION   DATMTW010000216 DATMTW010000000
VERSION     DATMTW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355691"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="hprK"
                     /locus_tag="VN084_04195"
     CDS             <1..781
                     /gene="hprK"
                     /locus_tag="VN084_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HWT28689.1"
                     /translation="SLQENELQQAIQNLFSTDLAAVVVANGEVAPSQLTKAADLYHTP
                     LFTSPLQSPGLMDVLSHYLAQAVAESVSRHGVFIEVQGFGVLIQGNPAIGKSELALEL
                     ISRGHRLVADDIVDFFRVAPDRLEGRCPELLQDFLEVRGLGILNIRALFGDNAVKPTK
                     PLDLIVQLEMADKMAPELLDRLKIKSQSEKILGVKVPKNTIPVAAGRNIAVLVEVAVR
                     NHMLLLRGINGTKQFMQRQHREMSRLAKSTTATPKKRRTIP"
     gene            781..1623
                     /gene="rapZ"
                     /locus_tag="VN084_04200"
     CDS             781..1623
                     /gene="rapZ"
                     /locus_tag="VN084_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWT28690.1"
                     /translation="MQLIILTGLSGSGKSVALRALEDSGYYCVDNLPATLLPQISQDL
                     SAAMQDRVAVSVDTRSAALEALPDNIQQLRDRGITVQVLFLESSVETLVKRFSETRRR
                     HPLSTGDESRTLAEHIRQERDLLSPLADLGHRIDTSNLSASKLRNWVRDFVANDTNGL
                     TLFFASFGFKHGIPLDADYVFDIRCLPNPHYDPVLRPLTGKDAPVRQFLESQETVGMM
                     YDDIQRFVARWLPCFIRDNRSYLTVAIGCTGGQHRSVYFVERLGEFFRNQQQKVLIRH
                     RELE"
     gene            1671..2084
                     /locus_tag="VN084_04205"
     CDS             1671..2084
                     /locus_tag="VN084_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HWT28691.1"
                     /translation="MIGILIIAHGTLGESLIHCASHVMGTRPPMLKQLGVGTHDDPAG
                     LLPQAQQMVKELDEGQGVLIMSDIYGATPCNLVSKLLVPGKVEGVAGVNLPMLVRALT
                     YRNGSILPLVEKALSGGHEGVVHFTKDTCRHHDQA"
     gene            2071..2340
                     /locus_tag="VN084_04210"
     CDS             2071..2340
                     /locus_tag="VN084_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWT28692.1"
                     /translation="MIKHEIEIINKLGLHARASTKLTQTASIYNSDIWIERNGRRVNA
                     KSIMGVMMLAASKGSKITLEAEGKDEQAAMEALIDLITSRFGEPE"
     gene            2522..4249
                     /gene="ptsP"
                     /locus_tag="VN084_04215"
     CDS             2522..4249
                     /gene="ptsP"
                     /locus_tag="VN084_04215"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWT28693.1"
                     /translation="MSFTMHGVAVSSGIAIGHAHLVSHALLEVVHYQVPKKLIEQEVT
                     RFEGAVDKVKQDLDVIRRQLPAGSPAEIGAFVNTHLMILADKHLSEEPKRIIREEQCN
                     AEWAIKQQMDDLVQQFEQIEDEYLRERKQDVVQVVERVIKVLLGHPSQVAVREQESAI
                     ILVAHDLSPADAIQFKQHQFAAFITDVGGATSHTAILARSLNIPSIVALQRARDLIRD
                     GELIIVDGNQGVVIANPDKDTLAEYRLRQEQWELEQQKLKRLKLTKAVTLDGTTIELH
                     ANIEVPEDVTQAKAAGATGVGLYRTEFLFMNRREMPDEEEQYRAYRKVAEAMKGMPVT
                     IRTLDLGADKQINPDTVRTCANPALGLRAIRLCLSEPHIFHTQLRAILRASHYGKIKI
                     LIPMLSNLSELRQAMLLLDRAKLSLRSEGLPFDEAIEIGGMIEVPAAAMCAEAFAKQL
                     DFLSIGTNDLIQYSLAIDRTDDAVSHLYNPLHPAILQLIEMTIRAGQKLGKPVSVCGE
                     MAGDTKLTRLLLGFGLRQFSMHPSHILAVKRQILQSDLPNLANVARKILGNQDIDKIE
                     PMLQKLNLA"
     gene            4340..>4667
                     /locus_tag="VN084_04220"
     CDS             4340..>4667
                     /locus_tag="VN084_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWT28694.1"
                     /translation="MSDVTTTPGANGPSADDKNIVVLTHLGGAVFSFIPSLIVWLLKK
                     DENAFIGEQAREALNFQVTVIIANIISWILMFLLVGFFLMAAVWIANIVLCIMAAVAA
                     SKGEPYR"
BASE COUNT         1018 a         1490 c         1341 g          818 t
ORIGIN      
        1 cagcctgcag gaaaatgagc tgcagcaggc gatccagaat ctcttttcca ccgaccttgc
       61 cgctgtcgtc gtcgccaacg gcgaagtcgc gccgtcgcag ctgaccaagg ccgccgatct
      121 ttaccatacg ccgctattca cctcacccct gcaaagcccg ggcctgatgg atgtgctgag
      181 ccattacctc gcccaggccg tggccgaatc ggtcagccgg catggcgtgt tcatcgaggt
      241 gcagggcttc ggcgtactga tccagggcaa tcccgccatc ggcaagagtg aactggcgct
      301 ggaactgatc tcgcgcggtc atcgcctggt cgccgatgac atcgtcgatt tctttcgcgt
      361 cgcgccggat cggctcgaag gccgatgccc tgaactgcta caggatttcc tcgaagtacg
      421 cggtctcggc atcctcaata tccgcgcgct gttcggcgac aacgccgtca agccgaccaa
      481 gccgctcgat ctcatcgtgc aactggagat ggcggacaag atggcgcccg aactgctcga
      541 ccgcctcaag atcaagtcgc agagcgaaaa gatcctcggg gtcaaagtcc ccaagaacac
      601 catccccgtc gccgccggcc gcaacatcgc cgtgctggtc gaggtcgccg tgcgcaacca
      661 catgctgctc ctgcgcggca tcaacggcac caaacaattc atgcagcgcc agcatcgcga
      721 gatgagtcgc ctcgccaaga gcacgacggc cacgcccaaa aaacggcgga ccatcccctg
      781 atgcaactga tcatcctcac cggtctctcc ggctcgggca agagtgtcgc actgcgggcg
      841 ctggaagaca gcggctacta ctgcgtcgat aacctgccgg cgaccctgct gccgcagatc
      901 tcgcaggacc tcagcgccgc catgcaggac cgtgtcgcgg tcagcgtcga tacccgcagt
      961 gcagcgctcg aagcgctgcc cgacaacatc cagcagctac gcgaccgagg catcacggta
     1021 caggtgctgt tcctcgaatc cagcgtcgag accctggtca agcgcttcag cgagacgcgg
     1081 cgccgccacc ccctgagcac gggagacgag agccgcaccc tggccgagca catccgccag
     1141 gagcgcgacc tgctgagccc gcttgcggac ctcggccatc gcatcgacac cagcaatctc
     1201 agcgccagca agttgcgcaa ctgggtacgc gacttcgtcg ccaacgacac taacggcctg
     1261 accctgttct tcgcttcgtt cggcttcaag cacggcatcc cgctcgatgc cgattacgtc
     1321 ttcgatatcc gctgcctgcc caacccgcat tacgaccccg tgctgcgccc gctcaccgga
     1381 aaggatgcgc ccgtgcggca gttcctggag agccaggaga ccgtgggcat gatgtatgac
     1441 gacatccagc gtttcgtcgc gcgctggttg ccttgtttca tccgcgacaa tcgtagctat
     1501 ctcactgtgg cgatcggctg cacgggaggg caacatcgct cggtgtattt tgtcgaacgg
     1561 ttaggggagt tttttcgcaa ccagcaacaa aaggtgctca tcaggcaccg cgaattagag
     1621 tagccgccac gattaccgtc gtgcacgctg ccaaagagga catacatatc atgataggaa
     1681 ttctgatcat cgcgcatggc acgctgggtg aaagcctgat ccattgtgcc agccatgtca
     1741 tgggcacgcg ccccccgatg ttgaagcaac tgggcgtcgg cactcatgac gacccggcgg
     1801 ggttgttgcc acaggcgcag cagatggtga aggagctgga tgaaggccag ggcgtactca
     1861 tcatgtcgga catctacggc gctacgccct gcaatctggt cagcaagctg ctggtgccgg
     1921 gcaaggtgga aggcgtcgcc ggggtcaacc tgccgatgct ggtacgggcg ctgacttatc
     1981 gcaacggcag catcctgccg ctggtggaaa aagcgctgag cggcggccac gaaggcgtcg
     2041 tacattttac caaggataca tgtcgtcacc atgatcaagc atgagataga aatcatcaat
     2101 aaactggggt tgcacgcgcg cgcctcgacc aagctgacgc agaccgccag tatctataac
     2161 agcgatatct ggatcgaacg taacggccgc cgcgtcaacg ccaagagcat catgggcgtg
     2221 atgatgctgg ccgccagcaa aggcagcaag atcacgctcg aagccgaggg caaggacgaa
     2281 caggccgcga tggaggcgct gatcgatctc atcaccagcc gtttcgggga gccggaatga
     2341 cgggaacaag cttgatgcgt catcagagta tttcattcct cggcttaact tactccgcag
     2401 cctcgtgcca tgcgaagtcg aggcacggcg ccaacgagcg aatggcgcag gcagtacatc
     2461 gagtacgaca aggaatgagc gaggccagca acaaagcatc gacgaatcag ggagtgaggc
     2521 gatgagtttt accatgcatg gggtggccgt ctcttcaggc atcgccatcg gccatgcgca
     2581 tctggtgtcg catgccttgc tcgaagtcgt gcattaccag gtaccgaaga aactcatcga
     2641 acaggaagtc acgcgtttcg aaggagccgt cgataaggtc aagcaggatc tcgacgtcat
     2701 ccgccgtcaa ttgccggcag gctcgccagc cgagatcggc gccttcgtca acacgcatct
     2761 catgatcctc gccgacaagc acctgtcgga ggagcccaag cgcatcatcc gcgaggagca
     2821 atgcaacgcc gaatgggcga tcaagcaaca gatggatgat ctggtccagc aattcgaaca
     2881 gatcgaggac gaatacctgc gtgagcgcaa gcaggatgtg gtgcaagtgg tcgagcgcgt
     2941 gatcaaggtg ctgctcgggc atcccagcca agtcgctgtc agggagcagg agagcgccat
     3001 catcctcgtg gcgcatgacc tgtcgccggc cgatgccatc cagttcaagc agcaccagtt
     3061 cgccgccttc atcaccgatg tcggcggcgc cacctcgcac acggcgatcc tggcgcgtag
     3121 cctcaatatc ccgtccatcg tcgccctgca gcgcgcgcgt gacctcatcc gcgacggcga
     3181 actcatcatc gtcgacggca accagggcgt ggtcatcgcc aacccggaca aggatacgct
     3241 cgccgaatac cggctgcggc aggaacagtg ggagctcgaa cagcagaaac tcaaacgcct
     3301 caagctgacc aaggcagtga cgctggacgg caccacgatc gagctgcatg ccaacatcga
     3361 agtcccggag gacgtcaccc aggccaaggc agcgggagcc actggcgtcg gcctctaccg
     3421 caccgaattc ctgttcatga accggcgcga gatgccggat gaagaagagc aataccgggc
     3481 ctaccgcaag gtggcggaag ccatgaaagg catgccggtg accatccgga cgctggatct
     3541 gggcgccgac aagcagatca acccggatac cgtgcgcacc tgcgccaacc cggcactggg
     3601 cctgcgcgcg atccgcctct gcctgtccga gccgcacatc ttccacaccc agttacgggc
     3661 gatcctgcgg gcatcgcatt acggcaagat caagatcctg atccccatgc tgtccaacct
     3721 ttcggagctg cggcaggcca tgctgctgct ggaccgggcc aagctcagcc tccgcagcga
     3781 gggactgccg ttcgacgaag cgatcgaaat cggcggcatg atcgaagtgc cggccgccgc
     3841 catgtgcgcc gaagccttcg ccaaacagct ggacttcctc tccatcggca ccaacgacct
     3901 gatccagtat tcgctggcga tcgaccgcac cgacgacgcc gtatcgcatc tctacaaccc
     3961 gctgcatccg gctatcctgc aattgatcga gatgaccatc cgcgctggcc agaaactggg
     4021 caaaccggtg tcggtctgcg gcgagatggc gggcgacacc aagctcacgc gcctgctgct
     4081 tggtttcggc ctgcgccagt tctccatgca tccctcgcat atcctcgccg tcaagcgaca
     4141 gatcctgcag agcgacctgc ccaacctggc gaacgtcgcg cgcaagatcc tgggcaatca
     4201 ggatattgac aagatcgaac ccatgctgca aaaactcaac ctggcctgac ggaggggaac
     4261 cacaggccgc tcgtatcttg aaggtcgttt aaccacagct gtttaaccac agttttataa
     4321 ccacaagggg agcatcatca tgtcagacgt caccaccacg ccaggcgcga atggaccaag
     4381 cgccgatgat aagaacatcg tcgtcctcac ccatctgggc ggcgcagttt tcagcttcat
     4441 cccgtcccta atcgtctggt tgctgaagaa ggacgagaac gcatttatcg gcgaacaggc
     4501 acgcgaggcc ttgaatttcc aggtcaccgt catcattgcc aatatcatca gctggatatt
     4561 gatgttcctg ctggtcgggt tcttcctcat ggctgcggtc tggatcgcca acatcgtgct
     4621 ttgcatcatg gccgcggtag ccgccagcaa gggtgaacca tatcggt
//
LOCUS       DATMTW010000217         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_388152, whole genome shotgun sequence.
ACCESSION   DATMTW010000217 DATMTW010000000
VERSION     DATMTW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388152"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VN084_04225"
     CDS             <1..195
                     /locus_tag="VN084_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWT28695.1"
                     /translation="NGDMDLAIAIRTGVIKSGMLYSQAGAGIVADSVPESEWMETQNK
                     ARAVLRAAEMVLAGLDNSGV"
     gene            198..>1577
                     /locus_tag="VN084_04230"
     CDS             198..>1577
                     /locus_tag="VN084_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HWT28696.1"
                     /translation="MPKNKGRWLHRLIIVALAYFATGKLGLLIPYVGSQVSLIWLPTG
                     ISVAALMRWGYGCWPAIYIAAFFVNLTVSGSPMLSACIALGNTLGPIAATWILKRFHF
                     SNHLNRPHDVLLMVVSAALGMLLSATGGVASLIYFDALPQGNTALAWVIWWIGDAVGV
                     LLVLPFLLNISRAELLRLWDQRAVYILWCGVASVLEWGIFQYVSDASGQFMLLAFVVL
                     PLVIWSAMRFGITGASLAVLGLSMIAVWATYNLHGPFFQLDTHQGIFALWVFMSTLAL
                     VALMITVQQSERLQSEIAMRSSEAQLRAVIDGALDAIVTIDAAGHLVEFNPAAERMFG
                     YMRADVIGRPLGEVIIPPSLRTAHIQGHQRFVATGEKHIFDRRIELTGMRADGSEFPI
                     ELTITSLRDKGLPLITGFMRDISERKQAEQEIRNLAFFDALTGLPNRRLLIDRLQQAF
                     ATSARSRSYG"
BASE COUNT          274 a          477 c          469 g          357 t
ORIGIN      
        1 aacggtgaca tggacctggc gatcgcgatc cgcacaggcg taatcaagag tggtatgctg
       61 tattcgcaag ctggtgctgg catcgttgcc gactccgtac cagagagtga atggatggaa
      121 acccaaaata aggcacgtgc agtgttacgt gccgcggaga tggtgctggc agggctcgac
      181 aacagcggag tctgagcatg cccaaaaata aaggccggtg gctacaccgg ctgataatcg
      241 ttgcacttgc ctactttgcg actggcaaac tgggtttgct cattccttac gtcggttcgc
      301 aggtttcact catctggctg cccaccggta tctcggtcgc ggcgctgatg cgctggggtt
      361 acggctgctg gcccgccatc tacatcgctg ccttcttcgt caatctcaca gtcagtggtt
      421 cgcccatgct ctcggcttgc atcgccctgg gcaatacact ggggccgata gccgcgacct
      481 ggatactcaa gcgcttccat ttcagcaatc atctcaatcg tccgcatgac gtcttgctga
      541 tggtggtctc cgccgcgctc ggtatgctgt tgtcggcgac gggcggcgtg gccagcctga
      601 tctatttcga tgccctgcca cagggcaata ccgcgctggc ctgggtcatc tggtggatcg
      661 gcgacgccgt gggcgtgctg ctggtgctgc cgttcctgct caatatctcg cgtgccgagc
      721 tcttgcggct ttgggaccag cgtgccgtct acatattgtg gtgcggcgtc gcttcggtac
      781 tggagtgggg catcttccag tacgtttcgg acgcatccgg ccaattcatg ctgctggctt
      841 tcgtggtgtt gccgctggtg atctggtcgg ccatgcgctt cggtatcacg ggtgcttcgc
      901 tcgccgtact gggcttgtcc atgatcgctg tgtgggcgac ctataacctg catggtccgt
      961 tcttccagct cgacacgcat cagggcatct ttgccttgtg ggtgttcatg agcaccttgg
     1021 cgctggtggc gctcatgatc accgtacaac agtcggaacg gctacagagc gagatcgcca
     1081 tgcgcagcag cgaagcgcag ctaagggcgg tcatcgacgg ggcgctcgat gccatcgtca
     1141 cgatcgatgc cgctggccat ctggtcgaat tcaatcccgc cgccgagcgc atgttcggct
     1201 acatgcgcgc cgacgtcatc ggccggccgc tgggcgaagt catcatcccg ccgagcctgc
     1261 gtacagccca tatccaggga catcaacgtt tcgtcgccac tggcgagaag catatcttcg
     1321 atcgccggat cgaattgacc ggcatgcgcg ccgacggcag cgagtttccc atcgaattga
     1381 ccatcacctc cctgcgcgac aaaggcttgc cgctgatcac cggtttcatg cgtgatatca
     1441 gcgagcgtaa acaggccgaa caagagatcc gtaacctggc cttcttcgac gcacttaccg
     1501 gcctgcccaa tcgccggctg ttgatcgacc ggttgcagca ggcttttgcc accagcgccc
     1561 gcagtcgcag ctatggc
//
LOCUS       DATMTW010000218         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_121072, whole genome shotgun sequence.
ACCESSION   DATMTW010000218 DATMTW010000000
VERSION     DATMTW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121072"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VN084_04235"
     CDS             <1..349
                     /locus_tag="VN084_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWT28697.1"
                     /translation="SAALLAALQLMRNDSSRHAHLQQLIRLLKTRLKLDRWRLMPSDT
                     AIQPLLVGSNEEAVRVSEWLLTKGLLVPAIRPPTVPTGTARLRISLSAAHSEAEILRL
                     VEALHEAEQALLA"
     gene            346..1155
                     /gene="bioH"
                     /locus_tag="VN084_04240"
     CDS             346..1155
                     /gene="bioH"
                     /locus_tag="VN084_04240"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HWT28698.1"
                     /translation="MSLHIETIGSGRPLVMIHGWGMHSGVWQPLVKRLSRHFNLHMID
                     LPGMGFSQPIEPGHLHAVAEKVAERMPPNADLCGWSLGGLVAMRIALSHPEQVRRMAL
                     IGSTPRFVNSPAGDVGAMDKPVAWTHGIDAKVFQDFATQVSQDYQNTLIKFLTLQCMG
                     ASDSRATIKQLRASFAERPVPTTGSLQKALRILLENDLREEIPDLRKPVLLMHGDRDT
                     LAPLQAAHWLAQHLPFGRLRVLAGASHAPFLSHPEQCIEALVQFLEPDHAG"
     gene            1160..2035
                     /gene="bioC"
                     /locus_tag="VN084_04245"
                     /pseudo
     CDS             1160..2035
                     /gene="bioC"
                     /locus_tag="VN084_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899482.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
     gene            2032..2706
                     /gene="bioD"
                     /locus_tag="VN084_04250"
     CDS             2032..2706
                     /gene="bioD"
                     /locus_tag="VN084_04250"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWT28699.1"
                     /translation="MSSAYFIAGTDTGVGKTLITSALVHGFAQRGLRSVGMKPVAAGC
                     TWQDGRLLSEDVVQLVAASNVEVDLDLINPYAFAPPIAPHIAADKAGVSIELPVIQRA
                     CKTLKGEVDRVMVEGVGGFRVPLGAELDTADLAQALGLPVVLVVGMRLGCINHTLLTL
                     EAIEVRGLTLAGWVANCIDPEMAVLDDNIAALRQRIPAPCLGIVPTLGTADFRLAATY
                     LQLDQL"
     gene            complement(2697..3347)
                     /gene="nadD"
                     /locus_tag="VN084_04255"
     CDS             complement(2697..3347)
                     /gene="nadD"
                     /locus_tag="VN084_04255"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWT28700.1"
                     /translation="MNAIGILGGTFDPIHFGHLRMAQELAEAVELDEVLFVPAAIPPH
                     RQQPATSAQHRAAMVAIAIQDNPRFRLDMRELLREGASYTIDTLQSLRLELGEQTSIS
                     LLLGSDAFLGLPGWHHWQELLDLAHIVVAHRPTAPLAEKIEKMHPQLQALCKERLTAD
                     AQALTQMRAGHLFMQHITPLDISATHIRGCFRAAESPRYLLPDSVIDYIVKEKLYS"
     gene            complement(3492..>3861)
                     /locus_tag="VN084_04260"
     CDS             complement(3492..>3861)
                     /locus_tag="VN084_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWT28701.1"
                     /translation="ALVKEAKAATDEDYYTEYLDAIISVKVVSGVDEAIAHINQYSSQ
                     HTEAIVTENYTKARQFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDKLHARGPV
                     GLEGLTSLKYVVLGNGEVRS"
BASE COUNT          714 a         1179 c         1215 g          753 t
ORIGIN      
        1 ctctgcggcc ctgcttgcgg cgctgcagct gatgcgaaat gattcttcgc gtcatgcgca
       61 tctacagcag ctgatccgtc ttttgaagac tcgactcaag ctcgaccgct ggcggctgat
      121 gccatccgat acggcgatcc agccattgct ggtgggcagc aatgaagaag cagtccgagt
      181 aagtgagtgg ttgctgacga aaggcctgct ggtaccggca atccgtccgc cgaccgtacc
      241 cacaggcacc gcacgtttgc ggatctcgtt atccgccgct cacagcgaag cggaaatcct
      301 gcggctcgtg gaggctttgc atgaggcaga gcaggcgttg ctggcatgag cttgcatatc
      361 gagaccatcg gtagcggccg ccccctggtc atgatccatg gctggggcat gcatagtggc
      421 gtctggcagc cgctggtgaa gagactctcc cgccatttca acctgcatat gatcgatctt
      481 ccgggcatgg ggttcagcca gccgattgag cccggacacc tgcatgcggt cgcggaaaag
      541 gtcgccgagc ggatgccgcc caacgccgat ctttgcggtt ggtcgctggg tgggttggtg
      601 gcgatgcgca tcgcgctcag ccatccggaa caggtgcggc gcatggccct gatcggcagt
      661 acgccgcgat tcgtcaacag cccggctggc gatgtcggcg cgatggacaa gcctgtcgca
      721 tggactcacg gtatcgacgc caaggtgttc caggatttcg ccacgcaagt gagccaggac
      781 taccagaaca cactgatcaa gttcctcacc ctgcagtgca tgggtgccag cgattcgcgc
      841 gccaccatca agcagctgcg cgcgagtttc gccgaacggc cggtcccgac cacaggttcg
      901 ctgcaaaagg ccttgcgcat cctgctcgag aacgacctgc gcgaagagat ccccgatctg
      961 cgcaagccgg tgctactcat gcatggcgac cgcgatacgc tggcgccttt gcaggctgca
     1021 cactggctgg cgcagcactt gccattcggt cggttgcgcg tgctggccgg cgcctcacat
     1081 gcgccgttcc tgtcgcatcc ggaacaatgc atcgaggcgc tggtgcagtt cctcgagcca
     1141 gatcatgccg gatgaacgca tgaacgataa ctaccaactc gacaaggcgc gcgtacgtgc
     1201 ctcattcgac cgtgccgccg gcacttacga tgccgctgcg gtcttgcaga cagaagtgcg
     1261 tcagcgcatg ttcgagcggc tcgaactcat caagctgcag ccgcagtgtg ttctcgatgc
     1321 gggttgtggc accggccacg gtagcgaact gctcagtcaa cgtttcccga cggcggatgt
     1381 actatcgctc gatatcgcca tgggcatgtt gaaaaagacc cttgccagtc gctcgctgat
     1441 acagcgcctg ttcggtcgct catcccgcac cggtatctgt gccgacatcg aacacctgcc
     1501 gctggcgggc cagagtctcg atctgatctg gtccaatctc gcgatccagt ggtgcaacga
     1561 cctcgaccgc gcctttggcg agatgcatcg cagcctgcgt gccggcgggc tggtgatgtt
     1621 cagcaccttt ggcccggata cgttgaaaga gttgcgtgct gccgccagtg tggatgccgg
     1681 acacgtgcat gtcagccgtt tcatcgacat gcacgacatc ggtgatgcgc tcgtacgcgc
     1741 cggcttcagc gcgccagtac tcgatgtcga acacttcgtg ctcacctacg acgatgtcat
     1801 gggcgtgatg cgcgacctca aggccatcgg cgcgcacaac gctgcacagg gccgcaatcg
     1861 tggcctgctg ggccggggct tcctggaaaa gctgacagcc gcctacgaac gattccgcag
     1921 cgaaggcaag ctgccggcga cctactaagt ggtctatggt catgcctgga aacccgaacc
     1981 caaggccgac ttgccggacg gcctcgcccc tgtgacgttc cggagccgac aatgagtagc
     2041 gcctatttca tcgctggtac cgataccggc gtcggcaaga cgctcatcac cagtgcgctg
     2101 gtccatgggt tcgcgcaacg cggcttgcgc agcgtcggga tgaaaccggt cgcagcgggc
     2161 tgtacttggc aggatggacg cctgttgtcc gaggatgtcg tgcagctggt ggcggccagc
     2221 aacgtcgagg tcgatctcga tctcatcaat ccttatgctt tcgcgccacc gatcgcgccg
     2281 catatcgccg ccgataaggc aggggtcagt atcgaactgc cagtgatcca gcgggcctgc
     2341 aagaccttga agggagaggt tgatcgcgtc atggtagaag gggtgggcgg cttccgtgtg
     2401 ccgctcggcg ccgaactcga taccgccgat ctggcccagg cgctcggtct gccggtggtg
     2461 ctggtggtcg gtatgcggct gggttgcatc aaccatactc tgctgacgct ggaggcgatc
     2521 gaggtgcgcg ggctgacact cgctggctgg gtcgccaatt gtatcgatcc cgagatggcc
     2581 gtacttgatg acaatatcgc cgcactgcgg cagcgcatcc ccgcgccatg cctcggtatc
     2641 gtgccgaccc tgggtaccgc ggatttccgc ctggcggcga cctacctgca actcgatcag
     2701 ctgtagagct tctctttcac gatgtaatcg atgacgctat ccggcaacag gtaacgcgga
     2761 ctttcggcgg ctcggaaaca cccgcggata tgggtagccg agatgtccag cggagtgatg
     2821 tgctgcatga agagatgtcc ggcgcgcatc tgcgtcaagg cctgcgcatc cgcagtcaac
     2881 cgctccttgc aaagtgcctg tagctgggga tgcatcttct ctatcttttc ggccagcggc
     2941 gcagtggggc gatgggcaac cacgatatgc gccaggtcga gcagctcctg ccagtgatgc
     3001 cagcctggca ggccaaggaa ggcgtcgctc cccagcagta ggctgatcga agtctgttcc
     3061 ccgagctcca ggcgtagtga ttgcagagtg tcgatggtgt aggaagcgcc ttcacgcaat
     3121 agctcgcgca tgtccagccg gaaccgcggg ttgtcctgaa tcgcgatcgc gaccatggct
     3181 gcacgatgtt gcgcggaagt cgccggttgc tggcgatgcg gcgggatcgc cgcaggcacg
     3241 aacaagacct cgtccagctc cacggcctcg gccagttcct gcgccatgcg cagatgaccg
     3301 aagtggatcg gatcgaaggt gccgcctagg atgccgatgg cgttcacgtt gtgaggtgcc
     3361 ggatacgcga gcgagcggga aaaagcgcaa ggcgcacggc gcgcaggatg cgagacatat
     3421 cagagggata ggcgcgtgtg tgagcaccgc gcaacgcagc tatttgctcg cgtagccgca
     3481 tagacaagcg cctagctgcg gacctcaccg ttacccaaca ccacatattt cagggacgtc
     3541 agtccctcaa ggccgaccgg gccacgcgca tgcagtttgt cggtggagat gccgatctcg
     3601 gcgccaagcc cgtattcgaa accatccgcg aagcgcgtcg aggcgttgac catgaccgag
     3661 ctcgagtcca cctcgcgcag gaactgcctt gccttggtgt agttttcggt gacgatggcc
     3721 tcggtgtgct gggaggaata ctggttgatg tgggcgatgg cctcgtcgac gccgctcacc
     3781 accttgaccg agatgatggc atccaggtat tcggtgtaat agtcctcgtc cgtcgccgcc
     3841 ttggcttcct tcaccagggc c
//
LOCUS       DATMTW010000219         6250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_489408, whole genome shotgun sequence.
ACCESSION   DATMTW010000219 DATMTW010000000
VERSION     DATMTW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489408"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..856)
                     /locus_tag="VN084_04265"
     CDS             complement(26..856)
                     /locus_tag="VN084_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HWT28702.1"
                     /translation="MTIGMDAVDHARRFGGVRRLYGEAALQRFQHAHVCVIGIGGVGT
                     WAVEALARNAIGALTLIDLDHIAESNVNRQLHALDTEFGKAKVVAMAERIRHINPTCT
                     VHGIEEFITAQNVDAMLSRGYDVVLDAIDDARAKVAIAAWCRAHRMPLVTTGGAGGRL
                     DPTRIQTGDLARAKGDRLLAKVRNQLRRDHGFAKGEKAKFGIPCVYSDEQVRKPDASC
                     AVDEATVDGAAVAQVDNPLTGLNCAGYGSSVCVTAPFGMAAAALVLGLLQSPVPGPKA
                     "
     gene            complement(853..1731)
                     /locus_tag="VN084_04270"
     CDS             complement(853..1731)
                     /locus_tag="VN084_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28703.1"
                     /translation="MLLMPGNAIAADWQFVGSNATGKYYIDRSSVHWLNPQAFTIMTN
                     VIQRDEAELQTQIRIDCADSRYTYLSGRETRGDQQLSSFDKPRPPEAIVEGSMPHELK
                     AEYCDAPDTRAAQWTSIGKSKISEVFYDQASIKQSSDGERFVADTRVVPFDQKGDTYS
                     RMLFNCSEKTFIVLRLSRQKDGKQEQVFDKPQPPTPTSKTATLDKLAGLYCGKVSAPS
                     VNASEQACAEALSIVQAMEQQIRADVAGGGMRCRQMDNYLESVRNLQAVVEQYRCPID
                     GLDAFGQEIRDAGCSP"
     gene            complement(1778..2158)
                     /gene="acpS"
                     /locus_tag="VN084_04275"
     CDS             complement(1778..2158)
                     /gene="acpS"
                     /locus_tag="VN084_04275"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWT28704.1"
                     /translation="MIFGIGTDIVEVARIEDSLARFGDAFAQRILTEQEWLSFQQSKT
                     QGRFLAKRFAAKEAFAKALGTGLREPATFQNIGITHDDLGKPVLDLAPPLQKLLDKKG
                     IVQQHLSISDEKALAAAFVVLEKA"
     gene            complement(2159..2884)
                     /gene="pdxJ"
                     /locus_tag="VN084_04280"
     CDS             complement(2159..2884)
                     /gene="pdxJ"
                     /locus_tag="VN084_04280"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWT28705.1"
                     /translation="MIKLGVNIDHVATLRNARGTQYPSVVQAALRAEEAGADSITIHL
                     REDRRHIRDEDVHALRPLLQTKMNLEMAVTEEMLGIALQIKPQDVCLVPERREERTTE
                     GGLEVAGNLAAVSEACDRLGEAGIRVSLFIAPDIAQIDAAKAAGAPVIEIHTGKFADT
                     TGSVQRHELERIRAAAAHAQQLGLVVNAGHGLNIHNVHEIAAIPGIEELNIGHAIVAH
                     ALFVGWEYAIREMKALMVHAAAN"
     gene            complement(2895..3626)
                     /gene="recO"
                     /locus_tag="VN084_04285"
     CDS             complement(2895..3626)
                     /gene="recO"
                     /locus_tag="VN084_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWT28706.1"
                     /translation="MAVTPIHRQDNQPVYVLHTYPFKETSLVVELFTRDFGRVAAVAK
                     GARRPRSAMRGMLQSFQPLLGAWSGKAELKNLHSLEWGERLLLLQGEALICGFYLNEL
                     LLRLLPREDEHALLFDFYGETLRQLAEGGDLAPTLRRFELKMLQEMGYAVPLLHDEED
                     AEIVAERQYRYVAERGACAAGRDDAAKIGVQLAGQTLLDMARHEYSAVPTQQQSKQLM
                     RYLLAHYLGDKPLHTRQLLIDLQGL"
     gene            complement(3749..4657)
                     /gene="era"
                     /locus_tag="VN084_04290"
     CDS             complement(3749..4657)
                     /gene="era"
                     /locus_tag="VN084_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWT28707.1"
                     /translation="MTQQNESQGSADFRCGTVAIVGRPNVGKSTLLNHILGLKLSITS
                     RKAQTTRHRLLGIHTTEDTQYLFVDTPGFQQKHVNALNRNLNRTVTQVLTEVDIVLFV
                     IEPMHLGDADRKVLKLLPADKPVLLVVNKSDLMGDKNNLLPLIQDFDLEFPFAGIIPV
                     SAKKNLNLEALLQTVRGYLPQQPAIYGEDELTDKNERFLAAEMIREKVFRLLGDEIPY
                     SVAVEIEKFEQEGQLRRIHAAIIVDKDSQKPMLIGKGGAKLKQISTEARQDMEKLFGG
                     KVWLETWVKVKGGWADDERALKSLGY"
     gene            complement(4641..5333)
                     /gene="rnc"
                     /locus_tag="VN084_04295"
     CDS             complement(4641..5333)
                     /gene="rnc"
                     /locus_tag="VN084_04295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWT28708.1"
                     /translation="MAQTGLARLLGHDFKQPQLLTQALTHRSHGSPNNERLEFLGDSV
                     LNLAIADQLYQRYPKLPEGDLSRLRASLVKESTLSEIALALQLGESLRLGEGELKSAG
                     WRRPSVLADALEAIIGAVYIDSGYEAAQRLVNRLYAERLDTLDPKAVGKDPKSLLQEY
                     LQGQKIDVPEYAVLSTEGEAHCQTFRVECHIRKLDLRTQAEGTSRRAAEQAAAQLAYE
                     QLIRQGKHDAAK"
     gene            complement(5340..5738)
                     /locus_tag="VN084_04300"
     CDS             complement(5340..5738)
                     /locus_tag="VN084_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HWT28709.1"
                     /translation="MHKQRGMTFIGVVLLVAIIVFVATIAIKLTPAYLEFFAVQKVVQ
                     KIQNEPSFASMGQKEIMDSFYRSASIDNIASVKEENITIEKAEGGKPIVTIEYQKVIP
                     IVANVSALLDFSASTEPGHKPAPAAAVEGE"
     gene            complement(5759..>6250)
                     /gene="lepB"
                     /locus_tag="VN084_04305"
     CDS             complement(5759..>6250)
                     /gene="lepB"
                     /locus_tag="VN084_04305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWT28710.1"
                     /translation="SYGLRVPILNNTFFEIGHPQRGDVFVFHYPPDPSIDYIKRVVGV
                     PGDKIAYRDKRLYINGKALQTDYVDDYSYMASGLNMIRAKRYHEQLGAHKHDLLIEED
                     SITIDGEVEVPPGHYFAMGDNRDNSKDSRVWGFVPERNLVGRAFFIWWNFDNFGRIGT
                     RIN"
BASE COUNT         1135 a         1877 c         1941 g         1297 t
ORIGIN      
        1 cgcgacgatg agggtcggcc gcctttcagg ctttcgggcc tggcacaggg ctttgcagta
       61 gaccgagtac gagcgcggca gcggccatgc cgaacggtgc cgtgacgcag acggaagagc
      121 cgtagccggc acagttgaga cccgtgagcg gattgtcgac ttgtgctacg gccgccccat
      181 ccacggtcgc ttcatccacg gcgcagctgg catcgggctt gcgcacctgt tcgtcggaat
      241 agacgcaggg gatgccgaac ttggctttct cgcccttggc gaatccgtgg tcacggcgca
      301 gctggttgcg tactttcgcg agcaggcggt cgcccttggc cctggccaga tcaccggtct
      361 ggatgcgcgt ggggtcaaga cggccaccag cgccgcctgt cgtcaccagc ggcatgcggt
      421 gggcgcggca ccaggcggcg atcgccactt tggcgcgtgc atcgtcgatg gcgtcgagca
      481 ccacgtcata gccgcgcgac agcatggcat cgacattctg tgccgtgatg aattcctcga
      541 tgccgtgcac tgtgcaggtc gggttgatat ggcggatgcg ctctgccatg gcgacgacct
      601 tggctttgcc gaactcggtg tcgagcgcat gcaactggcg attgacgttg gattcagcaa
      661 tatggtcgag atcgatcagc gtcagcgcgc cgatcgcgtt gcgcgccagc gcttccaccg
      721 cccaggtgcc gacgccgccg atgccgatga cgcagacatg ggcatgctgg aagcgctgca
      781 gcgcagcctc gccatagaga cggcgcacgc cgccaaagcg ccgcgcatga tcgaccgcat
      841 ccatgccgat ggtcatgggc tgcacccggc atcgcgtatc tcctgaccga aggcgtcgag
      901 accatcgatc gggcagcggt attgctctac caccgcctgc aggttacgca ccgactcaag
      961 atagttgtcc atctgccggc aacgcatgcc gcccccagcc acatcggcac ggatctgctg
     1021 ctccatggct tgcacgatcg acagcgcttc cgcgcaggcc tgctcactgg cgttgacgct
     1081 aggcgcagag accttgccgc agtaaagtcc ggccagcttg tccagggtcg cggtcttgct
     1141 ggtcggcgtc ggcggctgcg gcttatcgaa cacctgctcc tgcttgccgt ccttttggcg
     1201 actcaggcgc agcacgatga aggtcttctc gctgcaattg aacagcatgc ggctgtaggt
     1261 atcgcctttt tggtcgaacg gcaccaccct ggtgtcggcg acgaagcgct cgccgtcact
     1321 ggactgcttg atcgacgcct ggtcatagaa cacctcggag atcttgctct tgccgatgga
     1381 cgtccactgt gcggcgcgcg tatccggcgc gtcacagtat tcggccttga gctcatgcgg
     1441 catcgagccc tcgacgatcg cctccggcgg cctgggcttg tcgaaactgg agagctgctg
     1501 gtcgccacgg gtctcgcggc cactgagata ggtgtagcgg ctatcggcgc aatcgatgcg
     1561 gatctgggtc tgcaattcgg cttcgtcgcg ctggatgaca ttggtcatga tggtgaaggc
     1621 ttgcgggttg agccagtgca cgctgctgcg atcgatgtag tacttgccgg tggcattgga
     1681 gccgacgaac tgccaatcgg ccgcgatggc gttgcctggc ataagcaaca tccatgcatt
     1741 cggcagcagc gcaagcatcc gcagccagcg cacgaggtca ggctttctcc aacacgacga
     1801 aggcggcagc caacgccttt tcgtcgctga tcgacaagtg ctgctggacg atgcccttct
     1861 tgtcgagcag cttctgcaat ggcggggcga gatcgagtac gggtttcccc aggtcatcgt
     1921 gcgtgatgcc gatgttctgg aatgtagccg gttcgcgcag gcccgtgccc agcgccttgg
     1981 caaacgcctc cttggcggcg aagcgcttgg cgagaaaacg cccctgggtc ttggactgct
     2041 ggaaagacag ccattcctgc tcagtgagga tgcgctgggc gaaggcatcg ccgaatcgcg
     2101 ccagggaatc ctcgatgcgc gccacttcga cgatatcggt accgatgccg aagatcattc
     2161 aattcgcagc cgcatgaacc atcaaggctt tcatctcgcg gatggcatat tcccagccta
     2221 cgaacaaggc atgcgcgacg atggcgtgac cgatattgag ttcctcgata ccggggatgg
     2281 ccgcgatctc atggacgtta tggatattga gcccatggcc ggcattgacc accagcccga
     2341 gttgctgcgc atgcgcagcg gcagcgcgga tgcgttccag ttcatgccgc tgcacgctgc
     2401 ctgtggtatc ggcgaatttg ccggtatgga tctcgatgac cggcgcgccg gcggccttgg
     2461 ctgcgtcgat ctgtgcgata tcgggcgcga tgaacaggga gacgcggatg cccgcttcgc
     2521 ccaaccggtc gcaggcctcg ctgacggcag ccagattgcc cgcaacctcc agcccgcctt
     2581 cagtggtgcg ctcctcgcgc cgttccggca ccaggcagac gtcctgtggc ttgatctgca
     2641 aggcgatgcc gagcatctct tcggtgaccg ccatctccag attcatcttg gtttgcagca
     2701 ggggacgcag ggcatggacg tcttcatcgc ggatatggcg acgatcctcg cgcaggtgga
     2761 tagtgatgct gtcggcaccg gcctcctctg cgcgcagggc ggcctgcacc acgctcgggt
     2821 actgcgtgcc gcgcgcgttg cgcagggtag ccacatggtc gatgttgacg cctagtttga
     2881 tcatgccagt gttctcagag tccttgtaga tcgatcagca attgccgggt atgcaacggt
     2941 ttgtcgccca ggtagtgggc aagcagataa cgcatcagct gtttgctttg ctgctgggtg
     3001 gggacagcgg aatattcgtg ccgcgccatg tccagcaagg tctgccccgc caattgtacc
     3061 ccgattttcg cggcgtcatc tcggcctgca gcacaggcgc cgcgctctgc gacatagcga
     3121 tactgacgtt cggccacgat ctcggcatcc tcttcgtcat gcaacaaggg caccgcatag
     3181 cccatctcct gcagcatctt caactcgaag cgccgcaggg tcggcgccag gtcgccgcct
     3241 tcggccagct ggcgcaaggt ttcgccatag aaatcgaaca gcagcgcatg ttcatcctcg
     3301 cgcggcaaca ggcgcagcaa caactcattg agatagaacc cgcagatcaa tgcctcgcct
     3361 tgcaacagga gcaggcgctc accccattcc aggctatgca ggttcttcag ttcggccttg
     3421 cccgaccagg cgccgagcaa gggctggaaa gactgcagca tgccgcgcat ggccgagcga
     3481 ggccgccgcg cacccttggc cacggccgcc acacgcccga aatcgcgcgt gaacagttcg
     3541 acgacgaggc tggtttcctt gaacgggtag gtgtgcagga cgtagacggg ctggttgtcc
     3601 tggcggtgga tgggagtgac ggccatcttt gaaggttaaa cgaagttagc tgactgtagg
     3661 cttgagcgtg acgacaagac ggaaggctga tgacagtgcg aatactacgg caatacctga
     3721 caatgccgta ttcagctcaa gcataagatc aataacccag actttttaag gcgcgctcgt
     3781 catccgccca gccacctttg actttcaccc aggtctctag ccagaccttg ccgccgaaga
     3841 gcttttccat gtcctgccgc gcctcggtgg agatctgctt gagcttggcg ccacccttgc
     3901 cgatgagcat gggcttctgg ctgtccttgt cgacgatgat ggccgcatgg atgcgacgca
     3961 gctggccttc ctgctcgaac ttctcgatct cgaccgcgac cgaataaggg atctcgtcgc
     4021 cgagcaggcg gaagaccttc tcacggatca tctctgcggc gaggaagcgt tcgttcttgt
     4081 cggtgagttc gtcctcgcca tagatcgccg gctgctgcgg cagataaccg cgcacggtct
     4141 gcaacagcgc ctccaggttc aggttcttct tcgcactgac cggaatgatg ccggcgaacg
     4201 ggaactcgag atcgaaatcc tggatcagcg gcagcaggtt gttcttgtcg cccatgagat
     4261 cggatttgtt caccaccagc agcaccggct tgtccgccgg caacagcttg agcaccttgc
     4321 ggtcggcgtc acccaggtgc atgggttcga tgacgaacaa cacgatgtcg acctcggtca
     4381 gcacctgcgt caccgtgcga ttgagatttc gattgagcgc attgacgtgc ttctgctgga
     4441 aacctggcgt atcgacgaag aggtactggg tatcctcggt ggtgtggatc cccagcaaac
     4501 ggtgacgcgt ggtctgcgcc ttgcgcgagg tgatgctcag cttcaggccg aggatatggt
     4561 tgagcagcgt cgatttgccg acgttggggc ggccgacgat ggcgaccgta ccgcagcgga
     4621 aatctgcgct accctgagac tcattttgct gcgtcatgtt tgccttgtct gatgagttgt
     4681 tcgtaggcca gctgcgcggc cgcctgttcc gctgcacggc ggctggtgcc ttcggcctgg
     4741 gtacgaagat cgagcttgcg gatatggcac tcgacacgga aggtctggca atgggcttcg
     4801 ccctcagtgc tcagcaccgc atattccggc acgtcgatct tttggccctg caggtattcc
     4861 tgcagcagcg atttcggatc cttgccgacg gccttcgggt ccagcgtatc aagtcgctcg
     4921 gcatacaggc ggttgaccag gcgctgcgca gcctcgtagc cgctgtcgat atagaccgcg
     4981 ccgatgatcg cttccagcgc gtcggccagc accgacggcc ggcgccagcc ggcgctcttc
     5041 aactcgcctt cgccaagacg cagcgattcg ccgagttgca aggcgagcgc gatctcagac
     5101 aaggtcgatt ccttgaccag gcttgcacgc aggcggctca aatcaccttc cggcagtttc
     5161 gggtaacgct ggtagagctg atcggcgata gcgaggttga ggacgctatc gccgagaaat
     5221 tccagacgct cgttattggg gctaccgtgg ctgcggtgcg tgagcgcctg ggtcaggagt
     5281 tgcggttgct tgaagtcatg gccgagtaat ctggccaggc cggtctgtgc catgcaggcc
     5341 tattcgccct cgaccgcggc cgctggcgcc ggcttgtggc cgggttcggt cgaagcgctg
     5401 aaatccagca gcgcactgac attggcgacg atggggatca ccttctggta ttcgatggtg
     5461 acgatgggct tgccgccttc ggccttctca atggtgatgt tctcttcctt caccgaggcg
     5521 atgttgtcga tcgacgcgct gcggtagaag gagtccatga tctctttctg ccccatgctg
     5581 gcgaacgaag gttcgttctg gatcttctgc acgaccttct gcacggcgaa gaactccaga
     5641 taggccggcg tcagcttgat ggcgatggtc gccacgaaaa cgatgatggc cacgagcagg
     5701 accaccccga tgaaagtcat tccgcgttgt ttgtgcattt caggctcctg attgatggct
     5761 agttgatcct agtgccgatt ctgccgaaat tatcgaaatt ccaccagatg aaaaacgccc
     5821 tgccgaccag attgcgctcc ggcacgaacc cccagacacg gctgtcctta ctgttgtcgc
     5881 gattatcacc catggcgaaa taatgtccgg gcggcacttc gacctcgcca tcgatggtga
     5941 tgctgtcctc ttcgatgagg agatcgtgct tgtgggcgcc gagctgctca tgataacgct
     6001 tggcccggat catgttgagg ccggaagcca tgtagctgta atcgtcaaca taatcggtct
     6061 gcaacgcctt gccgttgatg tagaggcgct tgtcacgata ggcgatcttg tcgccaggca
     6121 caccgaccac gcgcttgatg taatcgatgg acggatcggg cgggtaatgg aagacgaaga
     6181 cgtcgccacg ctgcgggtgg ccgatctcga agaaggtatt gttgaggatg ggtacacgca
     6241 ggccatagct
//
LOCUS       DATMTW010000220         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_624189, whole genome shotgun sequence.
ACCESSION   DATMTW010000220 DATMTW010000000
VERSION     DATMTW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624189"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /gene="recG"
                     /locus_tag="VN084_04310"
     CDS             <1..1763
                     /gene="recG"
                     /locus_tag="VN084_04310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWT28711.1"
                     /translation="PSQLATLKEGRRLRVFGEVRGGFFGWEMVHPQYRAVVPDEPVPE
                     SLTPVYPTTAGLTQPTLRKWIAWALAHGPLGETLPPPIYAGLGLPNYADSLTALHHPT
                     PEADLASLSERATPAWRRLAFDELLAQQLSMRKHYARRRSLGAPLLRPSTQLVAALLH
                     SLPFTLTDAQRKVAAEISHDLAQPHPMQRLLQGDVGSGKTIVAAMAALQAIESDWQAA
                     IMAPTEILAEQHHRKLQGWLAPLGIEVAWLSGSQNKRARTTALEAIASGNAKLVVGTH
                     ALFQDQVQFARLGLAIVDEQHRFGVQQRLQLRKKGQPEPHQLMMSATPIPRTLSMSYY
                     ADLDVSVIDELPPGRTPILTRLVADSRRGEVLARVHDACAAGSQAYWVCPLIEESEAL
                     QLKTAEETYAQLTADFPDLRVGLVHGRMKPVEKQAVMEAFSRNEIQLLVATTVIEVGV
                     DVPNASLMVIEHAERMGLSQLHQLRGRVGRGAARSSCILLFQNPLSETARARLKVIYE
                     STDGFEIAQADLKLRGPGEFLGVRQSGVPMLKIADLERDAALLQHARDLAETLLDKHP
                     QAVEAHLRRWLGQGEELVKV"
     gene            1819..2331
                     /locus_tag="VN084_04315"
     CDS             1819..2331
                     /locus_tag="VN084_04315"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HWT28712.1"
                     /translation="MRWLRSPMGSGAYRHWLVDGGSLTRRLQRRCTDFAVQGVRLTDA
                     RPRLDEAGLLCTSSRRLALLREVRLLCAGRPVVFAHSVLPHTSLRGPWLGLARLGERP
                     LGGQLFADPRVVRTPLEYKKLHPHHALYRRATAHLNVRPLAIWARRSVFSLGCAAILV
                     TEVFLPQVLE"
     gene            2352..3215
                     /gene="ubiA"
                     /locus_tag="VN084_04320"
     CDS             2352..3215
                     /gene="ubiA"
                     /locus_tag="VN084_04320"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWT28713.1"
                     /translation="MGRIAMKFDAYERLMRLDKPIGILLLLWPTLWALWIAGQGHPPW
                     QTVLIFAMGTLLMRSAGCVMNDIADRDYDGLVERTQLRPLATREVTVKEALTLAALLC
                     LAAFAFVVFLNRLTILLSFAALFLAATYPLTKRFLAIPQAYLGIAFGFGIPMAFAAVR
                     GLIPIEAWLMLLANVFWATAYDTEYAMVDRDDYRKIGIRSSAILFGRYDVPAIMLCYL
                     AMLGLLAYIGMRLGLHWPYYLGLTAATLLAVYHYFLIRRRVRAACFKAFLHNNWLGAA
                     VFAGIFASYWR"
     gene            3320..3760
                     /gene="rplM"
                     /locus_tag="VN084_04325"
     CDS             3320..3760
                     /gene="rplM"
                     /locus_tag="VN084_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWT28714.1"
                     /translation="MKTFSAKAHEVKRDWFVVDATDLVLGRLASEIAHRLRGKHKTIY
                     TPHVDTGDYIVVINADKIKVTGNKAGDKQYHRHSGYPGGISTTTFAKMQERFPGRALE
                     KAVKGMLPKGPLGYAMFRKLKVYAGEKHDHVAQQPQPLKIQSQA"
     gene            3770..4162
                     /gene="rpsI"
                     /locus_tag="VN084_04330"
     CDS             3770..4162
                     /gene="rpsI"
                     /locus_tag="VN084_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWT28715.1"
                     /translation="MIGKYNYGTGRRKSSVARVFLKSGKGNIIVNDKPADEYFSRVTS
                     RMILRQPLELTENTSSFDILVNVVGGGESGQAGAVRHGITRALIDYDAALKSALSKAG
                     FVTRDAREVERKKVGLRKARRRKQFSKR"
     gene            4275..>5193
                     /gene="argC"
                     /locus_tag="VN084_04335"
     CDS             4275..>5193
                     /gene="argC"
                     /locus_tag="VN084_04335"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWT28716.1"
                     /translation="MQSKKLKVGIVGGTGYTGVELLRLLALHPHIELKAITSRGDAGM
                     PVADMFPSLRGYVGLNFVDPSEADLGGCDVVFFATPNGIAMQQTRELLNKGVRVIDLA
                     ADFRIQDIPTWEKWYGMPHACPDLVADAVYGLPELDRARIRTAQLVANPGCYPTAVQL
                     GFLPLLEAGVVDTTHLIADAKSGVSGAGRKAEVHTLFAEAGDNFKAYGVNGHRHLPEI
                     SQGLTRMSGQAVGLTFVPHLTPLIRGIHATLYARLTKDTDLQALFEQRYADEAFVDVL
                     PKGAHPETRSVRGSNQCRIAVHRPQGGDTV"
BASE COUNT          982 a         1569 c         1600 g         1042 t
ORIGIN      
        1 atccgagtca gctggccact ttgaaagagg gccggcgcct gcgtgtgttc ggcgaagtgc
       61 gcggcggttt cttcggttgg gagatggtgc acccacaata ccgggcagtg gtcccggacg
      121 agccggtacc ggaatcgctg acgccggtct atcccacgac cgccggcctc acccagccga
      181 ccttgcgtaa atggatcgcc tgggccctgg cacatgggcc gctgggcgaa accttgccgc
      241 cgccgatcta cgccgggctt ggcctgccga attatgccga tagcctcaca gcgctacatc
      301 acccgacacc ggaagctgac ctcgccagct tgtccgagcg cgcgactccg gcctggcgac
      361 gactggcttt cgacgagctg ctcgcgcaac agctctccat gcgcaagcac tatgcccgtc
      421 ggcgcagtct aggcgcaccg ttactccggc catcaacgca gctggtggcc gccttgctgc
      481 atagtctgcc gttcaccctc acggatgccc agcgcaaggt cgcggccgag atcagccacg
      541 atctggcgca gccacacccc atgcagcgcc tgttgcaggg ggatgtcggt agcggcaaga
      601 cgatcgtcgc agccatggcg gcgctgcagg ctatcgagag cgattggcag gcggcgatca
      661 tggcgcccac cgagatcctc gccgaacagc atcaccgcaa gcttcagggc tggctcgcgc
      721 cgctcggcat cgaagtcgcc tggctctccg gcagccagaa caagagagcg cggaccacgg
      781 cgctggaggc catcgccagt ggtaacgcca aactcgtggt cggtacgcat gccttgttcc
      841 aggatcaggt gcagttcgcc aggctggggc tcgccatcgt cgacgagcag catcgctttg
      901 gcgtgcagca gcgactgcag ctacgcaaga aggggcagcc ggagccgcac cagctcatga
      961 tgagcgccac gcccatcccg cgcaccttgt ccatgagcta ctacgcggac cttgatgtct
     1021 ccgtgatcga tgaattgccc ccaggccgca cgccgatcct gacccggctg gtggctgata
     1081 gtcggcgagg ggaggtgctg gcccgagtgc acgacgcgtg tgcggctggc agccaggcgt
     1141 actgggtctg tccgctgatc gaggaatccg aggcgttaca gctgaagacg gcggaagaga
     1201 cctatgcgca actcacggcg gattttcccg atctgcgcgt cgggcttgtg catgggcgca
     1261 tgaagccggt tgagaaacag gcggtgatgg aagccttcag ccgtaacgaa atccaattgc
     1321 tggtggcgac cacggtgatc gaggtgggcg tcgatgtgcc gaacgcgagc ctgatggtga
     1381 tcgagcacgc cgaacgtatg gggctctcgc aactgcatca gctcaggggg cgggtcggcc
     1441 gcggcgctgc caggtccagc tgcatcctgc tgttccagaa cccgttgtcg gaaacggcgc
     1501 gggcaaggct gaaggtcatc tacgaatcga ccgacggttt cgagatcgcg caggccgatc
     1561 tcaagctacg tggcccgggc gaattcctcg gtgtccggca gagtggcgtg cccatgctca
     1621 agatcgccga tctggaacgc gatgccgcgc tgctgcagca tgcccgggac ttggcagaaa
     1681 cattgctgga caagcatccg caagctgtcg aagcccatct gcgaagatgg ctgggtcagg
     1741 gcgaagagtt ggtcaaagtc tgacaagtcc tgcgataatc ccgttgtgaa agtcttccct
     1801 gcatcctccc accctcgtat gcgctggttg cgcagcccca tgggcagtgg tgcttaccgg
     1861 cactggctgg tggatggcgg ctcgctgacg cgacgtctgc agcggcgatg caccgatttt
     1921 gccgtccaag gtgtgcggct caccgacgcc aggccgcggc tggacgaggc cggcctgctg
     1981 tgtacctcga gccggcgtct ggccttgctg cgcgaagtcc gtctcctgtg cgccggtcgc
     2041 ccggtggtgt tcgcgcacag tgtcctgccg cacacgagcc tgcgcgggcc atggctgggg
     2101 ctggcacgtt tgggcgagcg accactgggc ggccagttgt tcgctgatcc ccgcgtggtc
     2161 cgcacgccgc tggaatacaa aaaactacat ccgcatcacg ccttatatcg cagggccacc
     2221 gcccatctga acgtccggcc gcttgccatc tgggcgcgac ggtcggtatt cagcctcggt
     2281 tgcgccgcga tcctcgtcac cgaagtgttc ctgccgcagg tgctcgaatg agagggtcgg
     2341 gaatgagcag gatgggcagg atcgccatga agttcgacgc ctacgaacgg ttgatgcggc
     2401 tggacaagcc gattggcatc ctgctgctgc tatggccgac gttatgggca ctctggatcg
     2461 ccggccaagg tcacccgccc tggcaaacgg tcctcatctt cgccatgggc acgctgctga
     2521 tgcgttctgc cggctgcgtc atgaatgata tcgctgaccg cgactatgac gggcttgtcg
     2581 agcggaccca gttgcgtccg ctggcgacgc gcgaagtgac agtgaaggaa gcgctcacac
     2641 tggccgcact gttgtgcctg gcggcctttg ctttcgtggt gttcctcaac aggctgacca
     2701 tcctgctctc attcgccgcc ttgttccttg ctgccaccta tcccttgacc aagcgattcc
     2761 tcgccatccc gcaggcctac ctgggcatcg cttttggctt cggcatcccc atggcgttcg
     2821 ctgccgtgcg cggcctgatc ccgatcgagg cctggctcat gttgctggcc aacgtcttct
     2881 gggcgaccgc ctacgatacc gaatacgcga tggtggatcg cgacgactac cgcaagatcg
     2941 gtatccgttc ctcagccatt ttgttcggcc ggtacgacgt gccggcgatc atgctgtgct
     3001 atctggcgat gttggggctg ctggcatata taggcatgcg tttgggcttg cattggcctt
     3061 attatctggg gctgacggcg gccacgttgc tggcggtcta tcattatttc ctgatcaggc
     3121 gtcgtgtgcg ggccgcgtgt ttcaaggcct ttttgcataa caactggctc ggcgccgccg
     3181 tctttgccgg aattttcgcc agctattggc gatgagcccc gtcaaaacct gaaaaacacg
     3241 ccatatatcg tttgacaaac ggcaaggccg tgacataaaa tccgcctctt gattttttca
     3301 gcgattattg agtagtccga tgaaaacctt ttccgcaaaa gcgcatgaag tgaagcgcga
     3361 ctggttcgta gtagatgcta ccgacctggt gctgggccgt ttggcgagcg agattgcgca
     3421 ccgcctgcgt ggcaagcata aaaccattta cacaccgcac gttgatacgg gcgattacat
     3481 tgtcgtcatc aatgccgaca agatcaaagt cacgggtaac aaggctggcg acaagcaata
     3541 tcatcgtcac tccggttatc caggtggtat ctccaccacc acctttgcca agatgcaaga
     3601 gcgtttcccg ggccgtgccc tggaaaaggc tgtcaagggc atgttgccaa agggtccgct
     3661 gggttatgcg atgttccgca agctgaaagt ctacgctggc gagaagcatg atcatgtggc
     3721 acaacagcca caacctttga aaattcaaag ccaagcttaa ggataagcga tgatcggtaa
     3781 atacaactat ggtactggcc gccgcaagag ttcggtagcc cgtgtgttcc tgaagtccgg
     3841 caagggcaac atcattgtca atgacaagcc agccgacgag tacttctccc gcgtcacctc
     3901 gcgtatgatc ctgcgtcagc cgctggaact gaccgagaac accagcagct tcgacatcct
     3961 cgtgaacgtc gtcggtggcg gtgaatctgg ccaggctggt gctgtacgtc acggcatcac
     4021 ccgcgcgctg atcgattacg atgcggctct gaagagcgcc ttgtccaagg caggtttcgt
     4081 gactcgcgat gcacgcgaag ttgagcgtaa gaaagtcggc ttgcgcaagg cgcgtcgtcg
     4141 taaacagttc tccaagcgct aagcttccgg aagctgcgtt acaggaatat cgaaaaagcc
     4201 gcacttgttg cggctttttt gttatctacg ccgcatttgt taccatgcaa cctgggtaaa
     4261 ggcatcgaat gaccatgcaa agcaaaaaac tgaaagtagg gatcgtcggc ggtaccggtt
     4321 acaccggcgt tgaattattg cggctcttgg cgctgcaccc gcatatcgaa ctcaaggcca
     4381 tcacctcgcg cggggatgcc ggtatgccgg tggcagacat gttcccgagc ttgcgtggct
     4441 atgtgggcct caatttcgtc gatccttccg aggccgatct cggcggttgc gatgtcgtgt
     4501 tcttcgccac gcctaacggt atcgccatgc aacagacgcg cgagctgctc aacaaaggcg
     4561 tgcgggtgat cgatctggcc gcggatttcc gtatccagga tatcccgacc tgggaaaaat
     4621 ggtacggcat gccccatgca tgccccgatc tcgtggcgga tgccgtctat gggttgcccg
     4681 agctcgaccg tgcccggatt cggacggcgc agctggtggc taaccctggg tgctatccga
     4741 ccgcggtgca gctcggtttc ctgccattgc tcgaagccgg tgtcgtggat accacccatc
     4801 tgatcgcgga tgccaagtcc ggggtcagcg gcgcgggacg caaggccgaa gtacatacct
     4861 tgttcgccga ggccggcgat aacttcaagg cttacggagt caacgggcac cggcatctgc
     4921 cggagatcag ccaaggcttg acccggatgt cgggacaagc agtggggttg accttcgtac
     4981 cccatctgac gccattgatc cggggtatcc acgccacgct atacgccaga ctgaccaagg
     5041 ataccgatct gcaggccttg ttcgagcagc gctatgccga cgaagcgttc gtcgatgtgc
     5101 tacccaaggg cgcgcatccc gagacacgtt cggtgcgtgg atccaatcag tgccgcatcg
     5161 cagtgcatcg gccgcagggc ggggacacgg tgg
//
LOCUS       DATMTW010000221         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_657823, whole genome shotgun sequence.
ACCESSION   DATMTW010000221 DATMTW010000000
VERSION     DATMTW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657823"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..529)
                     /locus_tag="VN084_04340"
     CDS             complement(14..529)
                     /locus_tag="VN084_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWT28717.1"
                     /translation="MRFKIDLSKTCVTTIALLGLGGFSLMASANPDDLNQSPYMTEFN
                     QLDIDGSGSLSKTEASKDKSFSKSSFQAADVDSNEALTQSEYADYKSRMQSKEVNRLV
                     DDSTITAKIKANIIKEEGLKGLQISVETHKGIVQLSGFVDNRTQIVRAEEIAQSIQGV
                     KSVRNSLVVKG"
     gene            complement(653..1015)
                     /locus_tag="VN084_04345"
     CDS             complement(653..1015)
                     /locus_tag="VN084_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28718.1"
                     /translation="MYATTKNHWLTNLAAICVIIFSLTGSALFVTALLHPYQPALPGK
                     VLLPAPPEGISAEPLLPMQVDPMASDPLLSDPISADPEEAEPMRPSRIGVEPMPETRE
                     RFERARIMASYKAMHSIL"
     gene            complement(1131..2570)
                     /locus_tag="VN084_04350"
     CDS             complement(1131..2570)
                     /locus_tag="VN084_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWT28719.1"
                     /translation="MKIRDWLFWNWIESFTERLGGRVILTLSAAMVITLLAVMFTDRW
                     IASIGEQDMKVRWIRHNINMLNELRTNLLMAESAERGYLLTQRDVYVAPFNDAIQHAR
                     DNVKAIQGLVTEKTRTMQQDNQQAWLLAVAARLEAKANQMETTLRLTRLGKFDEAWQV
                     LQLDQGLVEMSKFLEYTQTLLDTQNEALRVLLVDRESSIVLARVALFGSVTILFLVVL
                     MVVRQLFAEMALRDRLRLQLAQELQNYETQLEATKDLLGTLALDYQSDVERERQKLAR
                     ELHDELGSILTATKMDVSWVIRKCKDTLPEITDKLKKTMRYLDQGIQFKRQVVQDLHP
                     SMISTFGLWPALRMLIEDMAERNQWQVDILLPEETRAQVSDTIGLVAYRLVQETLNNC
                     SKYAKAGKVSVHIMVELNFLKIEIEDDGVGMDLHTIDGTTHGLSGMRHRVMAIGGKME
                     LVSEPGQGVITQAMIPLKTAAQAEPPAAV"
     gene            2691..2855
                     /locus_tag="VN084_04355"
     CDS             2691..2855
                     /locus_tag="VN084_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWT28720.1"
                     /translation="MLHYSAVFFVIALIAAFFGFSGIAAGAAEIAKILFFIFLVVTVI
                     SLIAGIWFVK"
     gene            2916..3356
                     /locus_tag="VN084_04360"
     CDS             2916..3356
                     /locus_tag="VN084_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28721.1"
                     /translation="MFNSTKNPEIKENAEQLVDNAKTRVTRVAKDAAKEARSAAGKIG
                     RRAGSAGKQTRKEAAALLDTLREILDPRETTSRLEQLTETVIDHVADWKDVAQNEVTH
                     AYKSGKIKSRRFVHKRTLLALSIAVGAGALIGYLASGSSDTDHE"
     gene            3381..3938
                     /locus_tag="VN084_04365"
     CDS             3381..3938
                     /locus_tag="VN084_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWT28722.1"
                     /translation="MSALNPLPTPGQAFKMDIETIRANARKHVENGAQTAGYQADRDI
                     VIAQLNAALATELVCALRYKRHYFTATGMNSEAIANEFNVHAQEEQSHADMIAARIVQ
                     LGGEPDFSPDGLQSRSHADYVPCTDLREMIRENLVAERIAIDTYHELICYIGDRDPTT
                     RRMLEEILATEEEHADELSDWLKRA"
     gene            3988..4620
                     /locus_tag="VN084_04370"
     CDS             3988..4620
                     /locus_tag="VN084_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWT28723.1"
                     /translation="MKRILLVDDHSIVRQGLRNLIELESDMAVAGEAASGIEAIQLIR
                     NNAYDVVVMDISMPDKNGVDTLHDLKHVAPELPVLILSGFAEEQYALNLMRNGCKGYL
                     SKDAESEEIITAIRTIAKGKRYISSELAELMTNELSHPTEKQLHETLSDREFQVFFKL
                     ASGRSATEIADELCISVKTVSTYRTRILEKMSLKTNADLTYYAIKNQLIS"
     gene            4797..5222
                     /locus_tag="VN084_04375"
     CDS             4797..5222
                     /locus_tag="VN084_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWT28724.1"
                     /translation="MSHTENQHDFQPQRMRVLLVEDSILLRDTMMEILEGAFGLQFDG
                     IAATQADAIALLQQQAFDMLILDIELAQGNGFEVLKATQSDDYPYPQPVYIIFTNHAY
                     PQYRLLARQLGVEHFFDKSMDFDVAMETIEEEARKFAAR"
BASE COUNT         1130 a         1590 c         1506 g         1097 t
ORIGIN      
        1 tctcagcgtt ggttcaaccc ttgaccacga ggctattgcg taccgacttc acgccttgga
       61 tcgattgtgc gatctcttcg gcacggacga tctgcgtccg gttatcgacg aagccgctca
      121 actggacgat gcccttgtga gtctcaacgc tgatctgtaa acccttcagg ccttcttctt
      181 tgatgatgtt ggccttgatc ttggccgtga tcgtactgtc gtcgaccagg cgattgactt
      241 ccttgctttg catgcgcgat ttgtaatcgg catattcgga ttgcgtcagc gcctcattgc
      301 tgtcgacatc cgcagcctgg aagctcgact tgctgaagga cttgtccttg ctggcttcgg
      361 tcttgctgag cgagccgctg ccgtcgatgt ccagctgatt gaattcggtc atgtacgggc
      421 tttggttgag atcgtccgga ttggccgatg ccatcaggct gaagccaccc aggcccaata
      481 gcgcgatggt ggtgacgcat gtcttgctga gatcgatctt gaaacgcata tgctttctcc
      541 tcgggttgga cagcactccc tgtcgggagg ccgctcggtg atataccagt gatgcaatga
      601 ctctatatga aatgactctg tagcagaccc tgtagcgcgc cgctggcgcc ggtcacagga
      661 tggaatgcat ggccttgtag ctcgccatga tgcgggcgcg ttcgaaccgt tcgcgggtct
      721 ctggcatggg ttcgacgccg atccggctcg gtcgcatcgg ctcggcttcc tcgggatccg
      781 ccgagatcgg atcggacaat agcgggtcag aggccatggg gtccacctgc atgggcaata
      841 acggctctgc cgagatcccc tcaggtggcg ccggcaacag gactttgccc ggcaaggctg
      901 gttgatatgg gtgcagcaat gcggtgacga agagtgcgct gccagtgagg ctgaagatga
      961 tcacgcagat ggccgccaga ttggtcagcc agtggttttt tgttgttgcg tacatgtggg
     1021 ctcccatccg gtccttgaat gaaccgttcc acgacaaaga tggagccatg ttaaacacgc
     1081 ggcgcgcgca gggcagtccg cgcgcgtctg gttatcttgt cagaatccgc ctacaccgca
     1141 gccggcggtt cagcctgcgc ggccgtctta agcgggatca tcgcctgcgt gatgacgccc
     1201 tggccaggtt cgctcaccag ctccatcttg ccgccgatcg ccatcacgcg gtgacgcatg
     1261 ccagaaagcc cgtgggtggt gccgtcgatg gtatgcaggt ccatgccgac gccgtcgtcc
     1321 tcgatctcga tcttgagaaa gttcagctcg accatgatgt gcacgctgac cttgccagcc
     1381 ttggcgtact tgctgcaatt gttgagcgtc tcctgcacca gccgataggc gacgagcccg
     1441 atcgtatcgc tcacttgcgc acgcgtctct tccggcaaca ggatatcgac ctgccactga
     1501 ttgcgctcgg ccatgtcttc gatcagcatg cgtagcgctg gccacaggcc aaaggtgctg
     1561 atcatggacg gatgcaggtc ctgcaccacc tgacgcttga actggatccc ctggtcgaga
     1621 tagcgcatgg tcttcttcag cttgtcggtg atctcgggca gggtgtcctt gcatttgcgg
     1681 atgacccagg acacatccat cttggtcgca gtcaggatcg accccagctc gtcatgcagc
     1741 tcacgcgcca gcttctgtcg ttcgcgctcc acatccgact ggtaatccag ggccaatgtg
     1801 ccgagcaggt cctttgtcgc ttccagctgg gtctcatagt tttgcagctc ctgcgcaagt
     1861 tgcaaccgca gccggtcgcg caacgccatt tccgcgaaca gttgtcgcac caccatcagc
     1921 accaccagaa aaaggatggt gacactaccg aacaaggcca cccgcgccag cacgatactg
     1981 ctttcgcgat cgaccagcag tacgcgcaag gcttcattct gcgtatccag cagggtctgc
     2041 gtgtattcaa ggaacttact catctcgacc aggccctggt cgagctgcag gacctgccag
     2101 gcctcatcga acttgcccag ccgggtcagg cgcagggtcg tctccatctg gttggccttg
     2161 gcttccagcc gtgccgcgac ggccagcagc catgcctgct ggttatcctg ctgcatggtc
     2221 cgggtcttct ccgtcaccag cccctggatc gccttgacgt tgtcgcgcgc atgctggatg
     2281 gcatcgttga acggcgcgac atagacatcg cgctgggtca gcaggtaacc gcgctcggcg
     2341 ctctccgcca tcagcagatt ggtccgcagc tcgttgagca tattgatgtt gtgacggatc
     2401 cagcgcacct tcatatcctg ctcgccgatc gaagcgatcc agcgatcggt gaacatgaca
     2461 gccagcaggg tgatcaccat ggcagcagaa agcgtgagga tgacgcggcc acccagacgc
     2521 tcggtgaaac tttcgatcca attccagaac agccagtctc tgatcttcat tcgtgatttt
     2581 tctcaggatg taggtgttgt ctgacaggcc tttcagccat ttgccgctga acgtcccgcc
     2641 agtttcccca taaagtagcc actgtagcgt caatcaacga agggagcatc atgctgcact
     2701 attcagccgt attcttcgtc atcgccctga tcgcggcgtt cttcggattc agcggtatcg
     2761 ctgccggggc agccgagatc gccaagatcc tgttctttat atttctggta gtgaccgtga
     2821 tatcgctgat cgcgggtatc tggttcgtca aataacctag ccgtccagcg tagtcattat
     2881 tcaaccgaaa ccaattcaag gaaatggagc ataacatgtt taattcgacc aagaatcccg
     2941 aaatcaagga aaacgccgag caactggtgg acaacgccaa gacccgcgtc acccgtgtcg
     3001 ccaaggacgc ggcgaaagag gcgaggtccg ccgccggcaa gatcggccgt cgcgccggca
     3061 gcgccggtaa gcagacgcgc aaggaggcag cagccctgct cgacaccctg cgcgagatcc
     3121 ttgacccgcg ggagaccacg tcccgcctgg agcaactgac cgagacggtg atcgaccatg
     3181 tcgccgactg gaaggatgtc gcccagaacg aggtcaccca tgcctacaag agcggcaaga
     3241 tcaagtcgcg ccgcttcgtc cacaagcgca ccctgctcgc cctctccatc gccgtcggcg
     3301 caggtgccct gatcggctat ctcgccagtg gcagcagcga tacggatcac gaataatcag
     3361 acttaacgga aggaaaaaac atgtcagcac tcaacccatt accgacgccg ggacaggcgt
     3421 tcaagatgga catcgaaacc atacgcgcca atgcccgcaa gcatgtggag aatggcgcac
     3481 agacggcagg ttatcaggcc gaccgcgaca tcgtcatcgc ccagctcaac gccgccctgg
     3541 ccaccgagct ggtctgcgcg ctgcgctaca agcggcatta cttcaccgcc accggcatga
     3601 attcggaggc gatcgccaac gaattcaacg tgcacgccca ggaagaacag tcgcatgccg
     3661 acatgatcgc ggcacgtatc gtgcaacttg gcggtgaacc tgacttctcg ccggatggcc
     3721 tgcagagccg tagtcatgcc gattatgtgc cctgcaccga tctgcgcgag atgatcaggg
     3781 aaaatctggt ggccgagcgc atcgccatcg acacctatca tgaattgatc tgctatatcg
     3841 gcgacaggga cccgaccacg cgccgcatgc tggaagagat cctggcgacc gaggaggaac
     3901 acgcggatga gttatccgac tggctcaaac gggcatgagg tcgccgcgca tgggtatact
     3961 gtggctttgg acccgaatcg acgtcatatg aagagaatct tgctggtcga cgatcacagc
     4021 atcgtgcgcc agggcttgcg taacctgatc gagctggaat ccgacatggc ggtggcgggt
     4081 gaagccgctt ccggcatcga ggcgatccag ctcatccgta acaacgccta tgatgtcgtg
     4141 gtgatggata tctcgatgcc ggacaagaat ggcgtcgata cgctgcatga cttgaagcat
     4201 gtggcacccg agctgccggt gctgatcctg agcggtttcg ccgaggagca gtatgcgctg
     4261 aacctcatgc gtaacggctg caagggctat ctgtcgaagg acgcggaatc ggaggagatc
     4321 atcaccgcca tccgcaccat cgccaagggc aagcgctata tctcatccga gctggcggaa
     4381 ctgatgacca acgagctcag ccacccgacc gagaagcaac tgcacgagac gctctccgac
     4441 cgcgagttcc aggtgttctt caaactcgcc agcggccgca gcgcgacgga gatcgccgac
     4501 gagctctgca tcagcgtcaa gacggtgagc acctatcgca cgcgcatcct cgagaagatg
     4561 agcttgaaga cgaacgccga cctcacttat tacgcgatca agaaccagct gatcagctga
     4621 aatccggcca ggcgaacaac tagggcgaac gatagacata acgctcgcgt tgctgacctg
     4681 agcacatgca cagaatgatg caaaatgggt aagaccacgt aggacagaac ctgatagccg
     4741 gcctttaagg gagcctttag aatcctgtag gaagtcagag aatggattta cacaatatgt
     4801 cacatactga aaatcagcat gattttcagc cgcaacgcat gcgggtattg ctcgtggaag
     4861 actcgatctt gctgcgcgac accatgatgg agatcctcga aggggccttc ggtctgcaat
     4921 tcgacggcat cgccgcgacg caagccgacg ccatcgcatt gctgcagcaa caggctttcg
     4981 acatgttgat cctggatatc gaactggctc agggcaacgg cttcgaagtg ctcaaagcca
     5041 cacagtctga cgactatcct tatccgcagc cggtctatat catcttcacc aaccacgcct
     5101 atccgcagta tcgtctgctg gcccggcagc tgggcgtcga gcatttcttc gacaaatcga
     5161 tggatttcga cgtcgccatg gaaaccatcg aagaggaagc gcgtaaattc gccgctcgct
     5221 aacatagctc gctaacatag cttgttgcgg tgggagaccc tcccccgcct ggcaacggga
     5281 cgacccgttg tctgcgtcat agtccgggac gacccgacga cca
//
LOCUS       DATMTW010000222         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_153137, whole genome shotgun sequence.
ACCESSION   DATMTW010000222 DATMTW010000000
VERSION     DATMTW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153137"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..500
                     /gene="sdhC"
                     /locus_tag="VN084_04380"
     CDS             90..500
                     /gene="sdhC"
                     /locus_tag="VN084_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWT28725.1"
                     /translation="MSEAAKKSRPQFSNIHVTQLMHYRLPLAGVVSILHRISGALMFL
                     LLPFVLYLLEQSLASDAAFASMKSFVAHPLAKLIILALSWAYLHHFAAGIRHLFMDMH
                     MGLSKEGSRHSAASVLAISLFLTLLVALKLFGVF"
     gene            505..876
                     /gene="sdhD"
                     /locus_tag="VN084_04385"
     CDS             505..876
                     /gene="sdhD"
                     /locus_tag="VN084_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWT28726.1"
                     /translation="MAIKHNNIGSKRLVVGAHYGFRDWLAQRVTAIVMALYTVIILVT
                     FLTGSNFSYEGWAGMFARQWFKIATFVTLLVMFYHVWVGMRDIWMDYVKPVVARLLLQ
                     SATIVWLIGCAGYAAQILWRV"
     gene            879..>1670
                     /locus_tag="VN084_04390"
     CDS             879..>1670
                     /locus_tag="VN084_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWT28727.1"
                     /translation="MAAIKSSIPSRRFDVFIVGAGGSGMRASLQLAEAGLNVAVLSKV
                     FPTRSHTVAAQGGVGASLGNMSEDNWYWHMFDTVKGGDYLGDQDAIEFMCREAPKVVY
                     ELEHFGMPFDRNPDGTIYQRPFGGHTANFGEKPVQRACAAADRTGHAMLHTLYQRNVR
                     AKTHFFVEWMAIDLIRDADGDVLGVVALEMETGDVMMLEAKTTILATGGAGRIWAAST
                     NAFINTGDGMGMAARAGLPLEDMEFWQFHPTGVAGAGVLITEGVRG"
BASE COUNT          290 a          554 c          493 g          333 t
ORIGIN      
        1 gatatctgtg cgagcggcca taataactat tggtgtgctg cacaaaaatc ggcgaaaatc
       61 gatgattatt tgttagaaga ggtgtttgta tgtcagaagc cgcaaaaaaa agccggccgc
      121 agttcagcaa tatccacgtc acgcagctga tgcactaccg cctgccgctg gctggcgtgg
      181 tttccatcct gcatcgcatc agcggcgcgc tgatgttcct gctgctgcct ttcgtgctct
      241 acctgctgga gcagagcctg gcctccgatg ccgcgttcgc cagcatgaag tccttcgtcg
      301 cgcatccgct ggccaagctg atcatcctgg cgctgtcctg ggcctacctg caccacttcg
      361 ccgccggcat ccgccacctg ttcatggaca tgcacatggg cctgagcaag gaaggctcgc
      421 gccactcggc tgccagcgtg ctggccatca gcctgttttt gaccctcctg gtcgcactga
      481 aactgttcgg agtgttctaa catcatggcc atcaagcaca acaacatcgg ctccaagcgc
      541 ctcgtcgtcg gcgcccacta tggctttcgc gactggctgg cccagcgcgt caccgccatc
      601 gtcatggccc tctataccgt catcatcctg gtgaccttcc tcacaggcag caatttcagc
      661 tatgaaggct gggccggcat gttcgcccgc cagtggttca agatcgccac cttcgtcacc
      721 ctgctcgtga tgttctatca cgtctgggtc ggcatgcgcg acatctggat ggactatgtc
      781 aagcccgtcg tcgcccgcct cttgctgcaa agtgccacca tcgtgtggct catcggttgc
      841 gccggctatg cggcgcagat tctctggaga gtgtaatcgt ggcagcaatc aaatcttcca
      901 ttccttcccg tcgcttcgat gtgttcatcg tcggcgcggg cggttccggc atgcgcgcct
      961 cgctgcaact ggcggaggcc ggcctgaacg tggcggtgct gtccaaggtc ttcccgaccc
     1021 gctcccacac cgtggccgcg cagggcggcg tcggcgcctc gctgggcaac atgtccgagg
     1081 acaactggta ctggcacatg ttcgacaccg tcaagggcgg cgactacctg ggcgaccagg
     1141 acgccatcga gttcatgtgc cgcgaagcgc ccaaggtcgt ctacgagctc gagcacttcg
     1201 gcatgccgtt cgaccgcaat cccgacggca ccatctacca gcgcccgttc ggcggccata
     1261 ccgccaactt cggcgagaag ccggtgcagc gcgcctgcgc cgcggccgac cgtaccggcc
     1321 atgcgatgct gcatacgctg taccagcgca acgtgcgcgc caagacccac ttcttcgtcg
     1381 agtggatggc catcgacctg atccgcgacg ccgacggcga cgtgctgggc gtggtggcgc
     1441 tggagatgga aaccggcgac gtgatgatgc tcgaagccaa gaccaccatc ctggccaccg
     1501 gcggcgccgg gcgcatctgg gcggcatcga ccaatgcctt catcaacact ggcgatggca
     1561 tgggcatggc tgcccgcgcc ggcctgccgc tggaagacat ggagttctgg cagttccacc
     1621 cgaccggcgt tgccggcgcc ggcgtgttga tcaccgaagg cgtgcgcggc
//
LOCUS       DATMTW010000223         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_456225, whole genome shotgun sequence.
ACCESSION   DATMTW010000223 DATMTW010000000
VERSION     DATMTW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456225"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VN084_04395"
     CDS             <1..1218
                     /locus_tag="VN084_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWT28728.1"
                     /translation="LLSKRDFALCLTDMRLPDGNGLDLVRYISQHHPGLPVAVITAYG
                     SADNAVSALKAGAFDYLSKPISLKQLRPLVESALKLHAPGMPASGGLKLIGESAAITQ
                     ARAMIEKLARSQAPVYISGESGSGKELAARLIHLSSSRNDGPFIGVNCGAIPENLMES
                     EFFGYKKGAFTGALQDKAGMFHAASGGTLFLDEVADLPLAMQVKLLRAIQEKKVRMVG
                     GTQEESVDVRIISATHRNLSAMMEAGQFRQDLYYRLNVIELKMPALREMPEDIPLLAQ
                     SLLEKQCRQSGMPTPVISEEVQAMLMRYPFPGNVRELENVLERALALCDGKRIESEDI
                     LLKNAIRAKVPTDTNGMTLPDYLEDIEKQAILQALEKTNQNKTAAAKLLGVSFRTLRY
                     RLSKLRLSRESPD"
     gene            1272..2294
                     /locus_tag="VN084_04400"
     CDS             1272..2294
                     /locus_tag="VN084_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWT28729.1"
                     /translation="MTKRRLLLCGLLAIPLQAIPLHALAQPSQVELFELNNSIVQVVV
                     DLKNGRTGSGSGAVIDAFHVATNCHVLADSRGVAITKFHDNYNPSAIKADWKHDLCLL
                     KFNYALPFKPVQLRDSASLQYEEPVFAISYPNDTPVPQPSYGSVKAIYPYDGSVIIRT
                     NAAFAMGSSGGALFDQDFRLVGVTTFKSPGRKGYFYNLPVEWIKDLLNAPDTLTLDSL
                     DAPFWSLPEQERPYFMRVVIPYQQHEWAALKTTAQAWHSSDPQSAEACLYLGSAELHL
                     GETGAGIAHLRQAVNLKPRHLEALVSLGENAVERNAIQEAESIRAQIAQVDVDEAENL
                     GKLIQR"
BASE COUNT          517 a          738 c          705 g          414 t
ORIGIN      
        1 ctgcttagca agcgcgattt cgcgctctgc ctcaccgaca tgcgactacc cgacggcaat
       61 gggctcgatc tcgtccgcta catcagccag catcatccgg gattgccagt cgcggtgatc
      121 acagcctacg gcagtgccga caatgctgtc tccgcactca aggcaggggc attcgactac
      181 ctgagcaaac cgatctccct gaaacagctg cggccgttgg tggaatcagc gctcaaactg
      241 cacgcgcccg gcatgcccgc atcaggcggc ctcaagttga tcggcgaatc ggcggcgatc
      301 acacaggcgc gcgccatgat cgagaagctc gcccgcagcc aggcaccggt ctatatcagc
      361 ggcgagtccg gcagcggcaa ggagctcgcc gcacgcctga tccacctcag cagctcgcgc
      421 aacgacgggc cgttcatcgg ggtcaattgc ggcgcgatcc cggagaacct gatggagagc
      481 gagttcttcg gctacaaaaa aggggctttc accggcgccc tgcaggacaa ggccgggatg
      541 tttcacgctg ccagcggcgg cacgctgttt ctggatgaag tggccgacct gccgctggcg
      601 atgcaggtga aattgctgcg ggcgatccag gagaagaagg tgcgtatggt cggcggcaca
      661 caagaggaat cggtcgatgt gcgcatcatc agcgccacac accgcaacct gtcggccatg
      721 atggaggcgg gtcaatttcg gcaggatctc tattaccggc ttaacgtcat cgaactgaag
      781 atgccggcgc tgcgcgagat gccggaagat atcccgctgc tggcgcaaag cctgctggag
      841 aagcagtgcc gacagtcggg catgccgacg ccggtgatca gcgaggaagt gcaggcgatg
      901 ctgatgcgct atccgttccc gggcaacgta cgtgaacttg agaacgtatt agaacgtgcg
      961 ttggcgcttt gcgacggcaa gcggatcgag agcgaggaca tcctgctgaa aaacgcgatc
     1021 agggccaagg tgccgaccga caccaacggc atgaccctgc cggattacct tgaagacatc
     1081 gaaaaacagg cgatcctgca ggcgctggag aaaaccaacc agaacaagac cgccgccgcc
     1141 aaactacttg gcgtcagctt tcgcacgctt cgttaccgcc tctccaagct gcggctgagc
     1201 cgcgaatcgc cggattgaat cttttcgcgt tttttcgctc attgattcta taaccacccg
     1261 ggcgacgaaa catgacgaaa agacgattgc tgctttgcgg cctgctggcc atcccactcc
     1321 aggcgattcc actccatgcc ttggcgcaac ccagccaggt cgagctgttc gaactcaaca
     1381 actctatcgt gcaggtcgtg gtcgacctca agaacggccg aaccggcagc ggctccggcg
     1441 cggtgatcga tgccttccat gtcgccacca actgtcatgt tctggcagac agtcgtggcg
     1501 tcgccatcac caagttccat gacaactaca acccgagcgc catcaaggcc gactggaagc
     1561 atgacctctg cctgctcaaa ttcaactatg ccttgccctt caaacctgta cagctgcgtg
     1621 acagcgcgag cctgcaatac gaagaaccgg tgttcgccat cagttatccc aacgacacgc
     1681 cggtcccaca gccctcttac gggtcagtga aggcgatcta tccctatgat ggcagcgtga
     1741 tcatccgcac caatgccgcg ttcgccatgg gttccagcgg cggcgcgctg ttcgatcagg
     1801 atttcaggct ggtcggcgtc actaccttca agagcccggg ccgcaagggg tatttctaca
     1861 acctgccggt ggaatggatc aaggatttgc tcaacgcacc cgacacgctg acgctggaca
     1921 gcctggatgc gccgttctgg agcctgcccg agcaggaacg gccgtacttc atgcgcgtgg
     1981 tcatcccgta ccagcaacac gagtgggcgg cgctgaagac gacggcccag gcctggcaca
     2041 gcagcgatcc gcaaagcgcc gaagcctgct tatatctcgg tagcgcggaa ttgcatctgg
     2101 gcgagaccgg tgccgggatc gcgcacttgc gccaagcggt gaacctcaag ccgcgtcacc
     2161 tcgaagcgct ggtaagcctg ggcgagaacg cggtcgaacg caacgcgata caggaagcgg
     2221 aatcgatccg cgcgcagatc gcgcaggtgg atgtggacga ggcggagaac ctcgggaaac
     2281 tgatccaacg ctagccagca ggctacatcc cgactggcgg ggcgatagat ccggtcttgc
     2341 cctcatcggc cgaggctggc atcaacgcaa tcag
//
LOCUS       DATMTW010000224         7200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_221767, whole genome shotgun sequence.
ACCESSION   DATMTW010000224 DATMTW010000000
VERSION     DATMTW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221767"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /gene="ettA"
                     /locus_tag="VN084_04405"
     CDS             complement(<1..1368)
                     /gene="ettA"
                     /locus_tag="VN084_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWT28730.1"
                     /translation="MAQYVMSMLRVSKVVPPKKQIIKDISLSFFPGAKIGLLGLNGSG
                     KSTVLRIMAGADKEFEGEVQWQPGISIGYLPQEPELDPDKTVREEVESGLGEVMQAKQ
                     ELERIYAAYAEPDADFDKLAEEQAKYENILAASGSDVETQLEIAADALRLPPWEAKIG
                     PLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGHGIPWKGNYSSWLEQKDERIEQEGKQEVARQKAMKTELEWVR
                     QNPKARQAKSKARLSRYEEMSSVEYQKRNETQEIFIPPGERLGDKVIEFNGVSKGFGD
                     HLLIDNLSFSVPPGAIVGVIGPNGAGKSTLFRMLQGQEQPDSGEIVVGPTVKMAAVDQ
                     SRAGLPDDKSVFEAISGGADVITVGRFEMPSRAYIGRFNFKGGDQQKIVGQLSGGERG
                     RLHLAK"
     gene            complement(1437..2246)
                     /locus_tag="VN084_04410"
     CDS             complement(1437..2246)
                     /locus_tag="VN084_04410"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWT28731.1"
                     /translation="MQAHQATLPIDTQDGLYLDMQEAQRLGASLASSYTAAEPFPHIV
                     IDHFLPTVLIEEILANFPAEPTANEKHYERGYRGHHKRQISPNECSGYLRNVFAFFNS
                     APMLQFMEKLTSIDGLIPDPYFNGGGFHEIGTGGLLGVHSDFRINKTLHVERRLNAII
                     YLNKDWQDEYGGNLELWDKQMKHCVEKIAPIFNRCVIFNTDHDSNHGHPEPLNTPPDI
                     KRRSIALYYYTASKKLYDDISTHRTLYKRRPGDKIEWRKVLGGWFGRRDRD"
     gene            complement(2283..2519)
                     /locus_tag="VN084_04415"
     CDS             complement(2283..2519)
                     /locus_tag="VN084_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28732.1"
                     /translation="MKSTLFVIALLTLAGCDMLNTKPTVDTREWQEVSCSGFADWTKC
                     NEKAARMCPGGYEVAGREESVIAQRRIMKFACTK"
     gene            complement(2555..4405)
                     /gene="glmS"
                     /locus_tag="VN084_04420"
     CDS             complement(2555..4405)
                     /gene="glmS"
                     /locus_tag="VN084_04420"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWT28733.1"
                     /translation="MCGIVGAVSPNNVVPVLIEGLSRLEYRGYDSAGVAVLTSADKLA
                     DTEIRRVRAVGRVAELQTKSTAEQLTGHVGIGHTRWATHGGVTESNAHPHVSCGEIAV
                     VHNGIIENHDAQRSRLKALGYDFESQTDTEVIAHLIHHYHSQGERLLAATKKAVAELT
                     GAFAISVVSIKEPETMICARLGCPLLIGLGEGENFIASDVSAVLSVTRNVIYLEDGDV
                     AEIRCDGVTIYDSSDAVVERTIHRSDVSLASMELGPYAHFMQKEIHEQPSALTDTIEA
                     LIDDGQFSNALFGKDASEVFAQVDSILILAAGTSYYAGLTAKYWLEGIAKLPTSVEIA
                     SEYRYRQSVPNPKQLIVTISQSGETLDTMEALKHAKKLGQQLTLAICNVQESAIPRAS
                     RLILYTRAGAEIGVASTKAFTTQLVALFTLAATLAKQRGLLDKAQEDSYLDALRHLAG
                     SVQYALNLEPQIREWAKLFANKQHALFLGRGVHYPIALEGALKLKEISYIHAEAYPAG
                     ELKHGPLALVDGDMPVVVIAPNDALLEKVKSNMQEVKARGGELFVFADADSHFTESDG
                     VNVIRTPRHVGILSPVLHTIPVQLLAYHAALLKGTDVDKPRNLAKSVTVE"
     gene            complement(4452..5822)
                     /gene="glmU"
                     /locus_tag="VN084_04425"
     CDS             complement(4452..5822)
                     /gene="glmU"
                     /locus_tag="VN084_04425"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWT28734.1"
                     /translation="MPTLNIVILAAGKGTRMHSDRPKVLHALAGRALVGHVIDCAERL
                     SPQKIVVVHGYGGETVQQAFVGKPLLWAEQAQQLGTGHAVQQVLPYLDDDGVSLILLG
                     DVPLIEPVTCQALLDHATNSLALLTVMKDDPTGYGRIVRNEQGAVSAIVEHKDATETL
                     RAIKEVNSGIMAMPNRALKKWLAALGNDNAQGEYYLTDIVAMAVKDQMAVEAVVTTDE
                     WQVAGINSKQDLAAVERVYQQRRASALLRAGVTLADPARIDVRGALTVGRDVEIDVGC
                     IFEGSVSLGDGVKVGAYSILRETVVGPGTQIAPYTHMEQVKIANDCRIGPYARLRPGT
                     ELANETHVGNFVELKNAQVDVGSKINHLSYVGDSTVGKQVNIGAGTITCNYDGANKFR
                     TVIEDGAFIGSDSQLVAPVTVGAGATIGAGSTITKDAPAGELTLSRGRQTTIKGWKRP
                     VKKSGA"
     gene            5929..6720
                     /locus_tag="VN084_04430"
     CDS             5929..6720
                     /locus_tag="VN084_04430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWT28735.1"
                     /translation="MDLQLTDKLALVSGSTAGIGYAIARSLASEVAHVIVNGRSQQGV
                     DEAVASLRDETGGTLMGFAGDLSEAEVADAIHARFPKVEILVNNLGIFEPVPFEAIPD
                     ADWLRFFEVNVLSGVRLSRLYLPAMKQANWGRIIFISSESAVQIPAEMIHYGMTKTAQ
                     LAIARGLAESVAGTGITVNSVLPGPTKSRGVIDFVEALGAGQQKSFAEFETEFFQTVR
                     PSSLIKRFATPEEVAVMVAYVASPLASATTGAALRVDGGTIKSAF"
     gene            complement(6811..>7200)
                     /locus_tag="VN084_04435"
     CDS             complement(6811..>7200)
                     /locus_tag="VN084_04435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWT28736.1"
                     /translation="AEESIFSGLVEFVSAPAKMGEVGIYPRHAPMITNIKAGALRAKL
                     PDQAEETLIFVSGGILEVQPGVVTVLADTAIRGADLDQAKAEEAKRAAEEALKNNASE
                     IDYARAQAELAEAVAQLQAIERLRKKH"
BASE COUNT         1320 a         2137 c         2149 g         1594 t
ORIGIN      
        1 cttggcgagg tgcagacgac cacgctcacc gccggagagc tggccgacga tcttctgctg
       61 gtcgccgccc ttgaagttga accggccgat ataagcccgg ctcggcatct cgaaacgacc
      121 gaccgtgatg acgtcggcgc cgcccgagat cgcctcgaag acactcttgt cgtccggcag
      181 gccggcacgc gactggtcga ccgcagccat tttcaccgtg gggccgacga cgatctcacc
      241 gctatccggt tgctcctgtc cttgcagcat cctgaacagg gtggatttac cggcgccgtt
      301 agggccgatg acgccgacga tggcccccgg cggtacgcta aaactgagat tatcaatcaa
      361 gaggtggtcg ccaaaaccct tggagacgcc gttgaattcg atgaccttgt cgcccaggcg
      421 ctcgccgggc gggatgaaga tctcctgtgt ctcgttgcgc ttctggtatt cgaccgagct
      481 catctcctca taacgcgaga gacgtgcctt ggatttggcc tggcgcgcct taggattctg
      541 gcgcacccat tccagttcgg tcttcatggc tttttggcgc gcgacttcct gcttgccttc
      601 ctgttcgatc cgctcgtcct tctgctccag ccagctggag taattgcctt tccatgggat
      661 accgtgaccg cgatccagtt ccaggatcca ttcagcggca ttgtcgagga aataacggtc
      721 gtgggtgacc gcgaccacgg tgcccgggaa gcgcacgagg aactgctcca gccattcgac
      781 ggattcggca tcgaggtggt tggtcggttc gtcgagcagc aacatgtccg gcttcgacag
      841 cagcagtttg cacagcgcca cgcggcgttt ctcgccgccg gatagcggcc cgatcttcgc
      901 ctcccaaggc ggcagtcgca aggcatcggc agcgatctcg agctgggtct caacatccga
      961 gccgctggct gcgaggatgt tctcgtactt ggcctgctcc tccgccagct tgtcaaaatc
     1021 ggcgtcgggt tccgcatagg cagcgtagat gcgctccagc tcctgcttgg cttgcatgac
     1081 ttcgcccaag ccggattcga cttcctcacg tacggtcttg tccggatcga gctcgggctc
     1141 ctgcggcagg taaccaatgg agatgccggg ttgccactgg acctcaccct cgaactcctt
     1201 gtcagcaccg gccatgatgc gcagcacggt cgattttccc gagccgttga ggccgagcaa
     1261 gccgatcttg gcgccgggaa agaaggatag cgagatatcc ttgatgatct gtttcttggg
     1321 aggtacgact ttgctcacac ggagcatgga catcacatat tgggccatgg cgttttctga
     1381 agaggagtgc gaaaaaccgt tattttaacg cgcctgagcc aggacatggg cggcgatcag
     1441 tcgcggtcac gccgaccgaa ccagcccccc agcaccttgc gccattcgat cttgtcgccg
     1501 ggtctgcgct tataaagcgt gcgatgcgtg ctgatatcgt catacagttt tttcgatgcg
     1561 gtgtagtaat acaacgcgat cgatcggcgc ttgatatcgg gtggggtgtt caacggttcc
     1621 ggatggccgt ggttgctgtc gtggtcagtg ttgaagatga cgcaacgatt gaagatcggc
     1681 gcgatctttt ccacacaatg cttcatctgc ttgtcccaca gctccagatt gccgccatat
     1741 tcgtcctgcc agtccttgtt caggtagatg atggcattga ggcggcgttc cacatgcagg
     1801 gtcttgttga tgcggaaatc ggaatgcacg ccgagcaggc cgccggtgcc aatctcatgg
     1861 aagcctccgc cattgaaata gggatcaggg atgaggccgt cgatcgacgt cagcttctcc
     1921 atgaactgca gcatcggcgc cgaattgaag aacgcgaaca cattgcgcag atagccgctg
     1981 cattcattgg ggctgatctg tcgcttgtga tgtccgcgat agccgcgctc gtagtgtttt
     2041 tcgttcgcgg tcggttcggc cgggaaattc gccaggatct cctcgatcag cacagtcggc
     2101 aggaagtgat cgatgacgat gtgcgggaac ggctcggcag ccgtatagct ggacgcaagc
     2161 gaggcgccca gtcgctgcgc ctcctgcatg tcgagataca ggccatcctg cgtatcgatc
     2221 ggcagcgttg cctggtgagc ttgcatctgg cgtttcctcc cgggttgggt cagtgttgcc
     2281 tgttatttcg tacaggcgaa tttcatgatg cggcgctgcg cgataacgct ttcttcgcgg
     2341 ccggcgacct catagccgcc cgggcacata cgtgctgctt tctcgttgca cttggtccag
     2401 tcggcaaagc cgctgcagct gacttcctgc cactcccggg tatccaccgt aggcttggta
     2461 ttgagcatat cgcagccggc aagggtgagc agggcgatta cgaagagggt gcttttcatt
     2521 ttttttccag tcggttaagg gttgtgtttc ggttttattc cacggtgacc gattttgcca
     2581 ggttacgcgg cttgtctacg tccgtgccct tgagcaaggc cgcatggtac gccagcaact
     2641 gcaccgggat ggtatgcaac accggcgaca ggataccgac atggcgtggc gtgcggatca
     2701 cgttgacgcc atcgctttcg gtgaaatggc tatcggcgtc ggcgaacacg aacaactcgc
     2761 cgccacgcgc cttcacttcc tgcatgttgg atttcacttt ctccagtagc gcatcgttgg
     2821 gtgcgatcac cacgacgggc atatcgccat ccaccagtgc cagtgggccg tgcttgagtt
     2881 cgcctgccgg gtaggcctcg gcgtggatgt aggagatctc cttgagcttg agcgcgcctt
     2941 ccagcgcgat cggataatgc acgccgcgac cgaggaacag cgcatgttgc ttgttggcga
     3001 acaacttggc ccactcgcgg atctgtggct ccaggttcaa cgcatattgc acgctgcccg
     3061 ccagatggcg caacgcatcc agatagctgt cttcctgcgc cttgtcgagc agaccacgct
     3121 gtttggcgag cgtcgccgcc agcgtgaaca gcgcgaccag ctgcgtggtg aaggccttgg
     3181 tggaggcgac accgatctcg gcgccggcac gggtatagag aatgagccgt gacgcgcgcg
     3241 ggatggcgct ttcctgtacg ttgcagatgg caagggtcag ttgctggccc agcttcttgg
     3301 catgcttcag cgcttccatc gtgtccagtg tctcaccaga ctgcgagatc gtgacgatga
     3361 gctgtttcgg gttcggtacc gactggcggt agcggtactc gctggcgatc tcgacgctgg
     3421 tgggtagctt ggcgatgccc tccagccagt acttggcggt aagacctgcg tagtagctgg
     3481 tacctgcggc gaggatgagt atgctgtcga cctgcgcgaa cacctcgctt gcgtccttgc
     3541 cgaacaaggc attactaaac tgcccgtcat cgatcagggc ttcgatggta tcggtcaatg
     3601 cgctcggctg ctcgtggatc tccttctgca tgaagtgcgc atagggaccg agttccatcg
     3661 aggccaacga aacatcgcta cggtggatgg tgcgttcgac cacggcatcc gaactgtcgt
     3721 agatggttac gccgtcacaa cggatctcgg cgacatcgcc atcttccaga tagatgacgt
     3781 tgcgcgtcac ggaaagcacg gccgaaacat cagacgcgat gaagttctcg ccctcgccga
     3841 ggccgatcag caaggggcag ccgaggcggg cgcagatcat ggtctccggc tccttgatcg
     3901 acaccacgct gatggcgaag gcgccggtca attcggccac cgccttcttg gtcgctgcga
     3961 gcaggcgctc gccctggctg tgatagtgat ggatgagatg cgcgatgact tcagtatcgg
     4021 tctgcgattc aaagtcatag ccaagcgctt tgaggcgact acgctgcgcg tcatggttct
     4081 cgatgatgcc gttgtgcacg acggcaatct cgccacagga tacatgtggg tgggcgttgg
     4141 attcggtaac gccgccatgg gtcgcccagc gcgtatggcc gataccgaca tggccggtca
     4201 gctgttcggc ggtcgacttt gtctgcaact cagcgacgcg cccaacggca cgcacgcgcc
     4261 tgatctccgt gtccgcgagc ttgtccgcac ttgtgagcac agcgacaccg gccgagtcat
     4321 agccacgata ttcgagccga ctcaggccct cgatcaatac gggcacgaca ttgtttgggg
     4381 agactgcgcc gacgattcca cacattattc gcttaccttt ttgaggttca aatggccgat
     4441 gttcatccgg gctaggcgcc ggatttcttg accgggcgct tccagccctt gatcgttgtc
     4501 tgccggccgc gcgataacgt caattcgcct gccggcgcat ccttggtgat ggtcgagccg
     4561 gcaccgatgg ttgcgcctgc gccaacagtc acgggggcca ccagctggga atccgagccg
     4621 atgaacgcac cgtcctcgat caccgtgcgg aatttgttgg ccccatcgta attgcaggtg
     4681 atggtacccg cgccgatatt gacctgcttg cctacagtgc tgtcgccaac atagctcaag
     4741 tggttgatct tgctgccgac atccacctgc gcgttcttca gttcgacgaa gttgccgaca
     4801 tgcgtttcat tggccagctc cgtacccgga cgcaggcggg catatgggcc gatgcggcaa
     4861 tcgttggcga tcttgacctg ttccatatgg gtgtaaggtg cgatctgggt gccagggccg
     4921 accacagttt cacgcaatat actgtaagca cccactttca cgccgtcgcc caaggaaact
     4981 gaaccttcga agatacatcc gacatcgatc tcgacatcgc gtcctacagt cagcgcgccg
     5041 cggacatcga tacgcgccgg gtctgccaat gtcacgcccg ctcgcagaag cgcactagcc
     5101 ctacgctgct ggtacacgcg ttcgacggct gcgaggtctt gcttggaatt gatgccagcg
     5161 acctgccact catccgtggt cacgacagct tccaccgcca tctggtcttt cactgccatg
     5221 gcgacgatat cggtcaggta gtactctccc tgggcgttgt cgtttcccag tgccgccagc
     5281 cacttcttca gggcacggtt cggcatcgcc atgatgccgc tgttcacttc cttgatggcg
     5341 cgcagcgtct ctgtcgcgtc cttatgttcc acgatcgcgc tcactgcacc ttgttcgtta
     5401 cggacgatgc gtccgtaacc ggtcgggtcg tccttcatca ccgtgagtag cgcgagactg
     5461 tttgtggcgt gatcgagcaa ggcctggcat gtcaccggct cgatcaacgg cacgtcgccc
     5521 agcaggatca ggctgacgcc atcgtcatcg agatagggca ggacctgctg cacggcatgc
     5581 ccggtgccca gttgctgcgc ctgctctgcc cagagtagcg gtttgcccac gaaggcctgc
     5641 tgcacggttt cgccaccgta gccatggacc accacgatct tctgtggcga gagccgttcg
     5701 gcacagtcga tgacatgtcc aactagagct cggcctgcca gcgcatgcag gaccttgggc
     5761 ctgtcggaat gcatgcgtgt acccttgccg gcagcgagga tgacgatatt gagtgtgggc
     5821 atagggtgat ggttattcag gggtcgttcg agattttaac ggctttttgc ccgttacggg
     5881 cagccggttt accatgaaag ctatacccac cgatttttag gagcatgcat ggatcttcaa
     5941 ctcaccgata aactggcgct ggtctcgggc agtaccgccg gcatcggcta tgcgatcgcc
     6001 cggtcgctag ccagcgaagt ggctcacgtg atcgtcaacg gccgctccca gcaaggggtg
     6061 gatgaagctg tcgccagcct gcgggacgaa actggcggca cgctaatggg cttcgcgggc
     6121 gacctcagcg aggccgaagt tgcggatgcc atacacgcgc gcttccccaa ggtcgagatc
     6181 ctggtcaaca atctgggtat cttcgaaccc gttccattcg aggccatccc cgatgccgac
     6241 tggctgcgct tcttcgaggt caatgtcctg agtggcgtcc gtttgtcgcg gctgtatctg
     6301 ccggcgatga agcaggcgaa ctgggggcgc atcatcttca tctccagcga gagcgcggtg
     6361 cagatccctg ccgagatgat ccattatggc atgaccaaga ctgcccagct ggctatcgcg
     6421 cgcggattgg cagagtcggt cgctggaacc ggcataacgg tcaatagcgt gttgccgggg
     6481 ccaaccaaat cgcgtggtgt gatcgatttc gtcgaagcgc ttggagccgg tcagcaaaaa
     6541 tcattcgctg aattcgagac ggaattcttc cagacggtac gtcccagctc tctgatcaag
     6601 cgctttgcta cgccggagga agtggctgtc atggtcgcct atgtcgcaag cccccttgcg
     6661 tccgcgacca ctggggctgc gctacgtgtg gatggcggta cgattaagag tgctttttag
     6721 cgcgagagcg acctttacga acgcgggctc aaggctggaa aataaaaaag gcagctaaag
     6781 ctgccttttt catgaacatc ctagcgtagc ttagtgcttc ttgcgcaacc gctcgatcgc
     6841 ctgcagctgc gctacggctt ccgccagctc tgcctgggcg cgcgcataat cgatctctga
     6901 agcgttgttc ttcagggctt cctcagccgc gcgcttggct tcttcggcct tggcctgatc
     6961 gaggtctgcg ccacggatcg ccgtgtccgc cagcacggtg accacacccg gctgtacttc
     7021 caggataccg ccggagacga agatcaacgt ctcttcagcc tgatccggca gcttggcacg
     7081 cagggcgccg gccttgatgt tggtgatcat cggcgcgtga cgtgggtaga taccgacttc
     7141 acccatcttc gccggagcgg aaacgaattc caccaacccg gaaaaaatgg actcttcagc
//
LOCUS       DATMTW010000225         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_188085, whole genome shotgun sequence.
ACCESSION   DATMTW010000225 DATMTW010000000
VERSION     DATMTW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188085"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2065
                     /locus_tag="VN084_04440"
     CDS             143..2065
                     /locus_tag="VN084_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HWT28737.1"
                     /translation="MSRSSYIHRIRDAREHFLDRGEVPEGVLPESIQRSWNRCIQTGL
                     AIDLRPETEPADTTQLSELRARNSQLLSQAQPEMENLYAHISGTQSMVILSDADGVII
                     HALGDRDFMSKAQRVALQPGFSWREDISGTNAIGTALIEKNPVFVMGAEHYFERNGFL
                     NCSASPILDPHGNVIGVLDLSGDHRQPQEHTMALVRMSALMIENRLFNAHFLTDITIR
                     FHTRQEFIGTLWEGIAVFSPDGKLMAINRSGAFQLGLDEQKIAGVEFDQLFDLSLAKF
                     LDIARPALSSISKLTLRTGLRLFVRADPGMHTLASAISMPGHQSPAKAETPATPLDAL
                     DSGDTNMHGIITKARMALGHDIPMLIEGETGTGKELLARAIHESGPRKQGAFIAINCA
                     SIPEGLIESELFGHEEGAFTGARRRGAIGLIQQAHGGTLFLDEVGEMPLALQARLLRV
                     IQEREIVPLGSSKRIQVDIAVIAATNQPLRQRIAQNEFREDLYYRLNGLRLALPPLRE
                     RSDLDALIRRILDQLGKHDIRIRPDVLELMKRHPWRGNIRQLANVLRAAVVFMQDREL
                     AMHHLPEDFLEELKNLETPDASPPSHGNGDIASSELALIKYALNLHGGNMTAAARHLG
                     ISRATVYRKAKRLQLI"
     gene            2258..2851
                     /locus_tag="VN084_04445"
     CDS             2258..2851
                     /locus_tag="VN084_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28738.1"
                     /translation="MVYEKSYSGHQELINNQRALNARQRQVLIMIDGKRTQDDLARYL
                     QNLDVPEIIADLERQGYIHTPGKPGNSVKAPHHASGFGQAKLPPAAPTGVAQPQPNAS
                     SPAPIPASNDAGSASAQERPLDSGTLAAVKVILSISAKEHLGLMGRPMIEKIEKIQDR
                     AQLLTAISQWHMAIRESKSGRTTASVLIEEVNQLLNK"
BASE COUNT          619 a          959 c          803 g          496 t
ORIGIN      
        1 tatagcagat tgcatgcctg ccgcgaaagt tttattactg tctgattatt aatgtatttt
       61 ttgatggcag tcgccaacgc gctgtgtcat catctcaaaa tgagacattc aggtcgtgca
      121 gagtgagaca ccggagatcc ccatgagtcg ttccagctat atccaccgaa tccgtgacgc
      181 gcgcgagcac ttcctcgacc gcggcgaagt gccggaaggt gtactaccgg aatcgatcca
      241 gcgctcatgg aaccgctgca tccagacagg gctcgctatc gacctccggc ccgagaccga
      301 accagctgac accacgcaac tctcggaact gcgtgcgcgc aactcgcaac tgctgagcca
      361 ggcccagcca gagatggaga acctctacgc ccatatctcg ggcacgcaga gcatggtcat
      421 cctcagcgac gccgatggcg tcatcatcca cgcgctcggc gaccgcgatt tcatgagcaa
      481 ggcccagcgc gtcgcgctac agcccggatt ctcatggcgc gaagacatct ccggcaccaa
      541 cgccatcggc accgcgctga tcgaaaagaa ccctgtgttc gtcatgggcg cggaacacta
      601 tttcgaacgc aacggcttcc tcaattgctc ggcgtcgccc atcctcgatc cgcatggcaa
      661 cgtcatcggc gtactcgacc tgtccggcga ccaccgccag ccgcaggaac acaccatggc
      721 actggtgcgc atgtctgcgc tcatgatcga gaaccgcctg ttcaacgcgc atttcctcac
      781 tgacatcacc atccgtttcc acacgcggca agagttcatc ggcaccctgt gggagggcat
      841 cgccgtgttc tcgcccgacg gcaagctgat ggcgatcaac cgcagcggcg ctttccagct
      901 tggcctcgac gagcagaaga tcgctggcgt ggagttcgac cagctgttcg accttagtct
      961 cgccaagttc ctcgatatcg cccgcccggc gctatccagc atcagcaagc tgaccttgcg
     1021 caccggcctg cggctgttcg tgcgcgccga tcccggcatg catacgctcg ccagcgcgat
     1081 cagcatgccg gggcatcagt ctcctgccaa ggccgaaaca ccggcgaccc ctctcgatgc
     1141 gctggatagc ggcgacacca acatgcacgg catcatcacc aaggcgcgca tggcgctggg
     1201 gcacgacatc ccgatgctca tcgaaggcga gaccggcaca ggcaaggagc ttctggcccg
     1261 cgccatccat gaatccggcc cgcgcaagca aggcgcgttc atcgccatca attgcgcgtc
     1321 catccccgag ggactgatcg aatcggaatt gttcgggcat gaagaaggcg ccttcaccgg
     1381 cgcccgcaga cgcggcgcca tcggcctcat ccagcaggcg catggcggca ccctgttcct
     1441 cgatgaagtc ggcgagatgc cgctggcctt gcaggcacgg ttactgcgcg tgatccagga
     1501 gcgcgagatc gtaccgctgg gaagcagcaa gcgcatccag gtcgacatcg cggtgatcgc
     1561 cgccaccaac cagcccctgc ggcagcgcat cgcgcagaac gagtttcgcg aagaccttta
     1621 ttaccggctc aacggcctgc gcctggcgct gccgccattg cgcgagcgca gcgatctcga
     1681 cgcgctgatc cgccgcatcc tggaccagct cggcaagcat gacatccgta tccggccaga
     1741 tgtgctggag ctcatgaaac ggcacccgtg gcgcggcaac atccggcaac tggcgaatgt
     1801 gctgcgcgcc gcggtggtgt tcatgcaaga ccgggaactg gccatgcatc atctgccgga
     1861 agacttcctc gaagagctga aaaatctgga aacgccggat gcgagcccgc cctcgcatgg
     1921 caatggcgat atcgccagtt ccgaactggc gctcatcaag tacgcgctca acctgcacgg
     1981 tggcaacatg acggcagcgg cccgccactt gggcatcagc cgggcgaccg tgtatcgcaa
     2041 ggcgaagcgc ctgcaactga tctaggccgc taaccgttct taccccggct agttgcgccg
     2101 cgggtcgcgc cgaaaagaca cggcaagccg cacagactca agttcgcaag tcagccagct
     2161 ttcccatcag cactgcgtgc agtttgtcgc acaagcgaaa cacccgcgtc acaatcgccc
     2221 tttacccttg cgctgtcttt cattctcgag aagcgccatg gtctacgaaa aatcgtactc
     2281 gggtcaccag gaactcatca ataaccagcg agccctgaac gcccggcagc gtcaggtgct
     2341 gatcatgatc gacggcaagc gcacccagga cgatctggcc cgctatctgc agaatctcga
     2401 cgtgccggag atcatcgccg acctcgaacg ccagggctac atccatacgc cgggcaagcc
     2461 cggtaacagc gtcaaggcgc cccaccatgc ttcaggtttt ggccaggcaa aactgccccc
     2521 ggccgccccg actggagtcg cccagcctca gccgaatgca tccagccccg cacccatacc
     2581 tgcttcgaac gacgctggat ctgcgtcggc acaggagcgt ccacttgact ccggcacctt
     2641 ggctgcggtc aaggtcatcc tctctatcag cgcgaaagag catctgggcc tgatggggcg
     2701 gccgatgatc gaaaagatcg agaagatcca ggatcgggcg cagctgctga cggcgatctc
     2761 gcaatggcac atggccatcc gcgaatccaa atccggccgt accactgcct cggtgctgat
     2821 cgaggaagtg aatcagctgc tgaataaatg agaaataaaa cggaaattaa gaagcaa
//
LOCUS       DATMTW010000226         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_153364, whole genome shotgun sequence.
ACCESSION   DATMTW010000226 DATMTW010000000
VERSION     DATMTW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153364"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VN084_04450"
     CDS             <1..582
                     /locus_tag="VN084_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWT28739.1"
                     /translation="AMPRLLDGKTIAVARDEAFCFLYPANLDCLQALGARLTFFSPLH
                     DSALPEAEAVWLPGGYPELHMSALSANRGMRDSLRQALAGGMPMLAECGGMMALSASI
                     DDRPAFGLLPGKSRIDTKLQGIGTQTVELAGGRLHAHTFHHGIFDTSLPSWQTADSPY
                     GNGEAIYRSGSLLASFLHFYFPSNPAVAAGFFL"
     gene            579..1247
                     /gene="bluB"
                     /locus_tag="VN084_04455"
     CDS             579..1247
                     /gene="bluB"
                     /locus_tag="VN084_04455"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HWT28740.1"
                     /translation="MSDTHRYSEAERAAIYRVIRERRDMRHFIPQAVDQDALQRILQA
                     AHHAPSVGLMQPWRFIRISDRKLREQIHGLVDEERIKTACAIGEVENTTRMAEFFRLK
                     VEGILDCGELLVVALCDKRERHVFGRRTLPEMDVASVSCAIQNLWLAARAEGLGMGWV
                     SIFDPVKLGEALAMPAGAKPLALLCLGHVSTFYKEPMLVETGWAKERPLSDMLMENGW
                     QDGK"
     gene            1237..>1913
                     /locus_tag="VN084_04460"
     CDS             1237..>1913
                     /locus_tag="VN084_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWT28741.1"
                     /translation="MASNQQSLVGPQRIVCLTTETCEVLYLLGEQARIVGISGYTTRP
                     AIARKEKPKVGAFTSAKIDKILNLQPDLVLTCTDLQADIAASLIKAGIEVHAFNQRSV
                     AQIMQMITTLGALVGASDKASSLVTELEQAIAAVRESAALLPARPRVYFEEWDEPMIS
                     GIRWVVELIEIAGGVDCFAELSVQGSARDRVVDSEAVIARQPDIIIGSWCGKKFQPEQ
                     VINRPGW"
BASE COUNT          369 a          618 c          575 g          351 t
ORIGIN      
        1 gccatgccac gtttgctgga cggcaagacc attgccgtcg cccgtgacga ggcgttctgc
       61 ttcctctacc cggccaacct cgattgcctg caggcgctcg gtgcacgcct gacattcttc
      121 tcgccgctgc acgacagcgc gttgccggag gccgaagcgg tctggctgcc cggcggctat
      181 cccgaactgc acatgtctgc gctctccgcc aaccgaggca tgcgagattc cttgcgacag
      241 gcgctggccg gcggcatgcc catgcttgcg gaatgcggcg gcatgatggc cctatcggct
      301 tcgatcgacg acaggcccgc atttggactg ctgccaggca agagccgcat cgatacgaaa
      361 ctgcagggca tcggcacgca aaccgtcgaa ctggcgggcg gccgtctcca tgcgcacacc
      421 ttccatcacg gcatcttcga taccagcctg ccgtcatggc agactgccga cagcccttat
      481 ggcaatggcg aagcgatcta ccgttcaggt tcgctgctag cgagcttcct gcatttttac
      541 tttccctcca atccggcagt ggccgcggga ttcttcctat gagcgacacg caccgttatt
      601 cagaagccga aagggccgcc atctaccggg tgatccgtga acgtcgcgac atgcgccact
      661 tcattcccca ggccgtggat caggatgcgc tgcaacgtat cctgcaggcg gcacatcacg
      721 cgccgagcgt cggactcatg cagccttggc ggttcatccg catcagcgac cgcaagctcc
      781 gcgaacagat ccacggcctg gtggatgaag agcgcatcaa gaccgcttgc gccatcggcg
      841 aagtggagaa caccacacgc atggcggaat tcttccggct caaggtcgaa ggtatcctcg
      901 attgcggcga gttgctggtc gtcgccctgt gcgacaagcg cgagcgacat gtcttcgggc
      961 gtcgcacctt gccggagatg gacgtggctt ccgtcagttg cgcgatacag aacctgtggc
     1021 tggccgcacg cgccgagggc ctcgggatgg gctgggtatc gatctttgac ccggtcaagc
     1081 tgggcgaggc actggcgatg ccggcgggcg ccaaaccgct ggctctgctc tgtctcggcc
     1141 atgtcagcac cttctacaag gaacccatgc tggtagagac cggatgggcc aaggagcggc
     1201 cactgtccga catgctcatg gaaaacggct ggcaagatgg caagtaatca gcaatcacta
     1261 gtggggccgc aacgtatcgt ctgcctcacc accgaaacct gcgaggtgct ctatctgctc
     1321 ggcgagcagg cgcgcatcgt cggtatctcc ggttatacga ccagacccgc catcgcccgc
     1381 aaggagaagc ccaaggtcgg cgcgttcacc agcgccaaga tcgacaagat cctcaatctg
     1441 cagcctgatc tcgtcctgac ctgtaccgat ctgcaagccg atatcgccgc ctcgctgatc
     1501 aaggccggca tcgaagtgca tgccttcaac cagcgctccg tcgcgcagat catgcagatg
     1561 atcacgaccc tgggcgcgct ggtgggagca tcagacaaag cctcatcatt ggtcaccgag
     1621 ctcgaacagg ccatcgccgc tgtgcgcgaa tccgccgcct tgctaccggc cagaccgcgg
     1681 gtctatttcg aggaatggga cgagcccatg atcagcggta tccgctgggt agtggagctg
     1741 atcgagatcg cgggcggcgt cgactgtttc gccgagcttt cggtacaggg cagcgcaagg
     1801 gatcgtgtag tcgacagcga ggccgtgatc gcccgccagc cagatatcat catcggctcc
     1861 tggtgcggca agaaattcca gccggaacag gtcatcaacc gccccggctg gca
//
LOCUS       DATMTW010000227         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_422883, whole genome shotgun sequence.
ACCESSION   DATMTW010000227 DATMTW010000000
VERSION     DATMTW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422883"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VN084_04465"
     CDS             complement(<1..576)
                     /locus_tag="VN084_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HWT28742.1"
                     /translation="MSKRIIGLIMAALLTSSAYADEASLKKSIETVYPKLKVQSITKT
                     PYAGLYEVFLDGQIIYTDDKFSFLIAEGKVIDPVSRRNLTGERLDELNKVDFASLPLD
                     QAIKVVKGNGSRKLVVFSDPDCPFCKRLEQRELVNITDVTIYTFLFPLEQLHPDAANK
                     ARAIWCSADKSKAWTDWVLNNQLPKAAGNCDT"
     gene            complement(586..1041)
                     /locus_tag="VN084_04470"
     CDS             complement(586..1041)
                     /locus_tag="VN084_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWT28743.1"
                     /translation="MDEASLCQTVAAAGANVLGDLRLIGSPAAFPLNLQSTDRLVMPG
                     LVLTGDAAHQVHPLAGQGVNLGFRDVIQLANTLAERRPQEALGDLMLLRRYERSRKID
                     LVAMRHITHGLHELFGQPLPWMKRLRNLGLDFTNRQGWLKKQLIKHATT"
     gene            complement(1108..1764)
                     /locus_tag="VN084_04475"
     CDS             complement(1108..1764)
                     /locus_tag="VN084_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWT28744.1"
                     /translation="MAHVAEAMQAQIVVVGAGLVGAAAALALTQNGLHVMLVDGQPAT
                     SGLTPTEWDQRIYAITPGNQRWLNSLGIWQRMDSDRICKVERMQVWGDDCRSSLTFDA
                     YEASLPSLGFILEARQLEQALRLRLQELGVAMLPDSKVEALSFGGEQPELVLAGGQTL
                     KAPLIVAADGGNSVVRQYAGLTSMRHAYDELGVVANFTCALPHGRIARQWFREDGVLA
                     "
     gene            complement(1748..>2554)
                     /locus_tag="VN084_04480"
     CDS             complement(1748..>2554)
                     /locus_tag="VN084_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWT28745.1"
                     /translation="VEVLYDAEARLIEPGPHAAVVDFVVADQPHRQETSLALLADGGR
                     GLREIPGITRKTKEYGHDALVSIVRCELPHDNVAYERFTPAGPMALLPNGPHDFSLVW
                     TGASADIEQLLVLDDQSFLQSLHAQFGDRVGRFLTIGKRLSFPLRLSYLEPSTAPHLA
                     IIGNAAQTMHPVAGQGFNVGMRDAWELANVVSSHPVDAWGSETMLDSYRQQRRQDTRG
                     GVLFTDFLVNTFSNDVVGLSGLRALALSMLDVAQPLKHRLVRKMSYGARG"
BASE COUNT          534 a          769 c          716 g          535 t
ORIGIN      
        1 tgtatcgcag ttacccgcgg ctttgggcaa ctggttattg agtacccagt cggtccaggc
       61 cttcgattta tccgcagagc accagatcgc ccgggccttg ttggccgcat ccgggtgcaa
      121 ttgctcgaga ggaaacagga aagtatagat cgtgacatcc gtgatgttga cgagctccct
      181 ttgctcaagt cgcttacaga acgggcaatc cggatccgag aagaccacca gtttacggct
      241 gccgttacct ttcacgactt taatcgcctg gtccagcggt aggctggcga agtcgacctt
      301 gttaagttca tcgagacgtt cccccgtcag attgcgccgg ctcaccggat cgatgacctt
      361 gccttccgca atgaggaaac tgaatttatc gtcggtatag atgatctgcc catcgaggaa
      421 cacttcatac aatccggcat agggcgtctt tgtgatcgac tgcaccttca gcttgggata
      481 taccgtctcg atgctctttt tcaggctcgc ctcgtctgcg taggcactgc tcgtcagcag
      541 tgccgccatg atgaggccga tgattctttt ggacatcctg attccttatg tagtcgcgtg
      601 tttaatgagt tgcttcttca gccaaccttg ccggttagtg aagtccaggc cgagattgcg
      661 tagtcgcttc atccagggta gtggctgacc gaacagctcg tgcagcccgt gcgtgatatg
      721 tcgcatcgcc acaagatcga ttttgcgtga tcgttcataa cgcctgagta gcatcagatc
      781 gcccagagcc tcctgtggtc gacgctcggc aagtgtgttg gccagctgga tgacgtcccg
      841 gaatcccagg ttgacgcctt ggccagccaa ggggtggact tgatgcgccg catcaccggt
      901 caggacgagt cctggcatca ccaatcgatc ggtcgattgt agattgagcg ggaacgccgc
      961 agggctgccg atcaagcgca aatcacctag aacattagcg ccggcagcgg caacagtctg
     1021 gcaaagtgac gcctcgtcca tggaaagcaa ttcctcatgc ctgcgcgtcg accagaccat
     1081 ggagatacga ttcccgggta gcggtagtca tgccagtacg ccatcttccc ggaaccactg
     1141 acgtgcgatc cggccatggg gcaatgcaca tgtaaagttg gcgacgacgc caagttcatc
     1201 ataagcatgg cgcatgctcg tcaggccagc atattggcgc accacagagt tgccgccatc
     1261 tgcggcaacg atcagcggcg ccttgagcgt ctggccgcct gccagcacca attctggctg
     1321 ttcacctccg aaagacaagg cctcgacctt gctatccggc agcatggcga cccccagttc
     1381 ctgcaagcgc aaacgcaacg cctgttcaag ttggcgtgct tccaggataa aacccaggct
     1441 gggcaggctt gcctcatagg catcaaaggt cagcgaggat cggcagtcat ctccccacac
     1501 ttgcatgcgc tcgaccttgc agatgcgatc actatccatg cgctgccata taccgagcga
     1561 attcagccag cgctgattgc cgggcgtgat cgcgtagatg cgttgatccc attctgtcgg
     1621 tgtcagccca gatgtagcgg gttgaccgtc caccagcatc acgtgcaaac cgttctgcgt
     1681 gagcgcgagc gccgcagcgg cgccgaccag cccggcgccg accacgacga tttgcgcctg
     1741 catcgcctca gccacgtgcg ccatagctca tcttgcgtac caggcgatgt ttcagcggct
     1801 gcgcgacatc caacatgctg agcgcaagcg cacgtaatcc ggacaagcca acgacatcat
     1861 tcgagaaggt attgacgagg aagtcagtga acagcacgcc gccccgagtg tcctgacggc
     1921 gctgctggcg gtagctatct agcatcgttt cgctgcccca ggcgtcgacc ggatgtgacg
     1981 acactacgtt cgccagctcc caggcgtcac gcatgccgac attgaatccc tggccggcca
     2041 caggatgcat ggtctgggca gcattgccga tgatggcgag atgcggtgcc gtgctcggct
     2101 ccagatagga cagcctgagc gggaagctga gccgtttacc gatggtcaga aaacgtccta
     2161 cgcgatcacc gaactgggca tgcaggcttt ggaggaacga ctgatcgtcg agcacgagca
     2221 gctgttcgat atcggccgat gcacctgtcc ataccagtga aaaatcatgc ggcccattgg
     2281 gcagcagcgc cattggacca gccggcgtga atcgctcgta ggccacattg tcatgcggca
     2341 actcgcaccg cactatggaa acgagagcgt catgtccgta ttccttggtc ttcctggtga
     2401 tgcccgggat ctctctcaat ccacgaccac catccgccag cagggcaagg ctggtctcct
     2461 ggcgatgcgg ctggtctgcc acgacgaaat cgaccaccgc cgcatgtggc ccaggctcta
     2521 tcaacctggc ttcggcgtca tacaagactt ccac
//
LOCUS       DATMTW010000228         7598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_87880, whole genome shotgun sequence.
ACCESSION   DATMTW010000228 DATMTW010000000
VERSION     DATMTW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87880"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VN084_04485"
     CDS             complement(<1..578)
                     /locus_tag="VN084_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWT28746.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMEAQVLDSM
                     DLERERGITIKAQTAALQYKALDGQVYHLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQSVANCYTAIDLGVEVTAVLNKIDLPSADPERVIQEIEDVIGIEASDAVR
                     CSAKTGEGVRDVLEAVIAKVPPPVGDAEAPLKA"
     gene            complement(698..2125)
                     /locus_tag="VN084_04490"
     CDS             complement(698..2125)
                     /locus_tag="VN084_04490"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWT28747.1"
                     /translation="MLKKLIAVSAICLSFAGTAYSKDLPDFTVLAEQEGAAVVNIAVT
                     QAPQEEEGAMGPFQGMPDDDAMNEFFRRFGIPMPGVPGAPGGQPAPQQPSQAFGSGFV
                     IGSDGYILTNAHVVRNAADVVVKLSDKREFKAKVIGADTRTDVALLKIDATGLPKVNV
                     GDPNALKVGEWVVAIGAPFGLENTMTAGIVSAKGRALPQENFVPFIQTDVAINPGNSG
                     GPLFNLKGEVVGINSQIYSRTGGYMGLSFAIPIDVAIDISNQLKANGKISRGWLGIGI
                     QEITKELAESFGMKNTNGALVAGVEKGSPAEKGGLEAGDVITKFDGKAIVASSDLPRV
                     VGSTKPNKVVPVEILRKGSSKTLNIMLGEMPSDNEVVASSGKGPAKPEVNRIGLLLKE
                     LTPQQKRKLGGKNGLLVIDAQGAAAQAGIRRGDVVLGLNNTEVQSLEQFNKQIGATPS
                     GKTVALLVQRGDNTLYVPVKVGSDK"
     gene            complement(2135..2611)
                     /locus_tag="VN084_04495"
     CDS             complement(2135..2611)
                     /locus_tag="VN084_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HWT28748.1"
                     /translation="MIEEHAVVIGVERDIAMLEVVRRSACGLCGKTRGCGISLFGRLF
                     GHKNNVFKARNTIEARMGDQVVVAIEEHAVLMSAFIVYGVPLVALLLGAVVGHACSDG
                     SATQSDMYALAGAVTGLVLGLGWVKAYATARVMSHRYQPTILRLAEAQVVVNFKCE"
     gene            complement(2645..3607)
                     /locus_tag="VN084_04500"
     CDS             complement(2645..3607)
                     /locus_tag="VN084_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HWT28749.1"
                     /translation="MWRLLLGCLLATQFSYVNAAQDEDPWMVLQKAAQAARELSYQGV
                     FIYQSGKTARSVQITHMNYGQGEYARMFVLDGSPREVLSQGNDVVIFSPRDEKVVIEK
                     RRGQNLFPALLPIDIEAIKPSYQVRMGGTERIGGREGQIVYLEPRDKYRYGYKLWADR
                     EYGLLLKSASSNEHDQVMEQMAFNQLALLNTQNMDWFHPHIDPNKQYVMEEPANNRVS
                     TADAENWSVERLPAGYKQVDQIKRMVPGKNVPLTHIVFSDGLSSVSLFIEPLTRGTRP
                     KTGSTSLGATNIHAAVVEGHQIMVVGEVPEATVNQIANSVSFKK"
     gene            complement(3608..4057)
                     /locus_tag="VN084_04505"
     CDS             complement(3608..4057)
                     /locus_tag="VN084_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HWT28750.1"
                     /translation="MKDQISALMDDEFDVEGSGHLLTAVKAGGEMADCWSTYHLIGDA
                     MRGETYLRSDFTDRLMARLDSEPSLVESASQFRKRSSMMWSVAASVAAVMFVGWMVMQ
                     QQTQNAADAAPIEIAQSIPSEYLLAHQAYSANSAAYYIQPAAYESKR"
     gene            complement(4074..4676)
                     /gene="rpoE"
                     /locus_tag="VN084_04510"
     CDS             complement(4074..4676)
                     /gene="rpoE"
                     /locus_tag="VN084_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HWT28751.1"
                     /translation="MVGNREIDQELVERAQRGDKKAFGMLVDKYQRKLGRLLSRLIRD
                     PAEVEDVVQESFIKAYRALPNFRGESAFYTWLYRIGINTAKNYLVAMGRRPQVTTDIE
                     IEDAENFEDGNELRTMDTPETELMSKEIARTVNDTVAALPEELRTAITLREIEGLSYE
                     EIATLMDCPIGTVRSRIFRARETIAQKLRPLLDTPQDKRW"
     gene            4901..6535
                     /gene="nadB"
                     /locus_tag="VN084_04515"
     CDS             4901..6535
                     /gene="nadB"
                     /locus_tag="VN084_04515"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWT28752.1"
                     /translation="MQHYDVLIIGSGLAGLTLALKVADQKRVCLVSKRTINDSSSNWA
                     QGGIAAVLAGSDSIEAHIQDTLVAGAGLCDAEVTRMVAEHGRETVEWLVKEGVPFTRE
                     ADDSGFHLTREGGHSHRRIIHAADATGHAVQKTLAEKVREHANITLLEDHIAVDLITS
                     RKVKIEGNECLGAYVLDNASGKVLTIGADHTVLATGGAGKVYLYTTNPDVSTGDGIAM
                     AWRAGCRVANMEFIQFHPTCLYHPQAKSFLISEALRGEGGVLTLPDGSRFMQEHDARG
                     ELAPRDVVARAIDFEMKKRGIDCVYLDISHQPADFILSHFPTIYRRCMEFGIDITKQP
                     IPVVPAAHYSCGGIMTDHIGRTDLPRLYAIGETACTGLHGANRLASNSLLECLVFGQA
                     AARDILAQAPKPRVKLPYWDESRVTDADEEIIITHTWNELRRFMWNYVGIVRTTKRLM
                     RALHRIHMLRDEVHEFYSNFRISNNLIELRNLLQVAELIVLSAMERHESRGLHYSKDY
                     PDMLPDAIPTVLEPSNYYSLLDSDHGHRQRKAEHAR"
     gene            complement(6551..7312)
                     /locus_tag="VN084_04520"
     CDS             complement(6551..7312)
                     /locus_tag="VN084_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HWT28753.1"
                     /translation="MPAFQRYQLRFTSHIRDPRHQPKPDKVAARRMRVYTEIVFNNLY
                     GAVSACFPVAQKVLGKRAWTRLVRDFFAGHQATSPLFREIPQAFLSYLEKRQDLPPYL
                     MSLTHYEWIELAIASADIEDDMTRVDTHGDLLAGVPVFAAASALLRYDYPVHRISPRF
                     KPQAPLEEPVHFLVFRDAADMVRFIELNPVTARLLGLLQHAGQADAMTSRQALLQIAT
                     ELGHAEPEVIVGFGAQILHDLKGQGALLGIRLATV"
     gene            complement(7390..>7598)
                     /locus_tag="VN084_04525"
     CDS             complement(7390..>7598)
                     /locus_tag="VN084_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HWT28754.1"
                     /translation="DDLAIDTHGQDVIDPVWALLREAYDLYGVFPTLLERDTNIPPLT
                     RLVREIDYIAELQAPHRQAARAVQ"
BASE COUNT         1501 a         2344 c         2189 g         1564 t
ORIGIN      
        1 gccttgagcg gcgcttcagc gtcgccgacg ggcggcggta ccttggcgat gacggcctcc
       61 agcacatcgc ggacgccttc gccagtcttg gcggagcagc gtacagcgtc ggacgcctcg
      121 ataccgataa cgtcctcgat ctcctggatc acgcgctccg ggtcggcaga cggcaggtcg
      181 atcttgttca gtacggcagt gacttcgacg ccaaggtcga tcgcggtgta gcagttggcg
      241 acgctctgcg cctcgacacc ctgcgaggcg tcgaccacca gcaatgcgcc ctcgcaggcg
      301 gacagcgagc ggctgacttc gtaggagaaa tcgacgtgac caggcgtgtc gatcagattg
      361 aggtgataga cctgaccatc cagcgccttg tattgcaggg ctgcggtctg ggccttgatg
      421 gtgatgccgc gctcgcgctc cagatccatc gaatccagta cctgcgcctc catctcacga
      481 tcactgagtc cgccgcatag ctggatgata cggtccgcca gtgtcgattt gccgtgatcg
      541 atatgggcga tgatggagaa attacggata tttttcatga atgggtctgc aagctcacat
      601 ctggcgtgtt agcaagaagg gcgccgcagc gcccttctca atcgtctgaa ttaggtgcgg
      661 attttacatg aatgcccgct tgtccgcacc ttgtgagtta cttgtcgcta ccgaccttga
      721 ccggtacata cagcgtatta tcgccacgct gtacaagcag cgccaccgtc ttgccgctgg
      781 gggtggcacc gatctgcttg ttgaactgtt cgaggctttg cacctcggta ttgttcaggc
      841 caagcacgac atcgccacgg cggatgccag cctgtgccgc agcgccttgc gcgtcgatca
      901 ccagcaaacc gttcttgcct ccgagcttgc gcttctgctg tggcgtcaat tccttcagca
      961 gcaggccgat acggttcact tccggcttgg ccgggccctt gcccgagctc gccacgacct
     1021 cgttatcgct cggcatctcg cccagcatga tattcagggt cttgctcgat cccttgcgca
     1081 ggatctcgac cgggaccacc ttgttgggct tggtcgaacc gacgacgcgc ggcaggtcgg
     1141 atgaagcgac gatcgccttg ccatcgaact tggtgatgac gtcacccgct tcgaggccac
     1201 ccttctctgc cgggctacct ttttctacac cggcgaccag cgcaccgttg gtgttcttca
     1261 tgccgaacga ttcagccagc tccttggtga tctcctggat cccgataccg agccagccgc
     1321 gggagatctt gccgtttgcc ttgagctggt tggagatatc gatggctaca tcgatcggga
     1381 tggcgaacga cagccccata taaccaccgg tgcggctgta gatctgcgag ttgatgccga
     1441 ccacttcacc cttcaggttg aacagcggac caccggagtt accgggattg atggcgacgt
     1501 cggtctggat gaacggtacg aaattctcct gcggaagggc acggcccttg gcgctgacga
     1561 taccagccgt catggtgttc tcgagaccga acggcgcgcc gatcgctacg acccattcgc
     1621 ccaccttgag cgcgttggga tcgccgacat tgaccttcgg caggccggtc gcgtcgatct
     1681 tgagcagagc cacatctgta cgggtatcgg cgccgatgac cttggccttg aattcacgct
     1741 tgtcggaaag cttgacgaca acgtctgccg cgttccgcac cacatgggca ttggtcagga
     1801 tataaccgtc actgccgatg acgaagccgg agccgaatgc ctggctcggc tgttgcggcg
     1861 caggttggcc accaggtgcc cccggcacgc caggcatggg gatgccgaag cgacggaaga
     1921 actcgttcat ggcgtcgtcg tcaggcatgc cctggaaagg gcccatggca ccctcctcct
     1981 cctgcggcgc ctgggtcacg gcgatgttga cgacggcagc gccttcctgc tcggccagca
     2041 cggtaaaatc gggcagatcc ttggaatatg cagttcccgc gaaagacaga cagatcgcgg
     2101 agacggcaat caatttcttg agcatttctt cacctcattc acatttgaag ttaacaacga
     2161 cttgcgcttc ggccaaccgc aatatcgtcg gctgatagcg atgactcatg acgcgggcgg
     2221 tggcatacgc cttgacccaa cccaagccca gcacgaggcc agtgacggca ccggccaggg
     2281 catacatgtc cgattgcgtt gcgctgccat cagagcatgc gtgtccgaca acggcgccga
     2341 gcaatagtgc gacgagcgga acgccataga cgataaatgc gctcatgagc acggcatgct
     2401 cttcgatggc gaccaccacc tgatcgccca tgcgagcctc gatcgtattg cgcgccttga
     2461 acacgttatt cttgtggccg aacaggcgcc caaacagcga gatgccgcat ccgcgcgtct
     2521 tgccgcagag gccgcaagca ctgcgacgga cgacttcgag catggcgatg tcgcgttcga
     2581 caccgatcac gacggcgtgt tcttcaatca tgggcggact ctggtatgga taggttgtac
     2641 tggtttactt cttgaaactg acgctgttgg cgatctggtt cacggtcgct tccggcactt
     2701 cgccgaccac catgatctgg tgaccttcga ccaccgcggc atggatattg gtcgcgccaa
     2761 ggctggtgga accagtcttg ggtcgcgtgc cgcgcgtcaa cggctcgatg aacagcgaga
     2821 cactggacaa gccgtcggag aagacgatat gggtcagggg tacgttcttg ccaggcacca
     2881 tgcgcttgat ctggtctacc tgtttatacc ctgcgggcag gcgttctacc gaccagttct
     2941 cggcatcggc ggtggaaacg cggttgttcg ccggctcctc catcacatac tgcttgttag
     3001 ggtcgatgtg cgggtggaac cagtccatgt tctgcgtatt gagcaaagcc agctggttga
     3061 aagccatctg ttccatgacc tggtcatgtt cgttgctgct agcggatttc agcaacagac
     3121 cgtattcgcg gtcagcccat aacttgtagc cataacgata tttgtcgcgc ggctcgaggt
     3181 agacgatctg gccttcgcgg ccaccgatac gctcggtacc acccatgcgc acctgataac
     3241 tgggcttgat ggcttcgata tcgatcggca gtagcgcggg aaaaaggttc tgaccacgac
     3301 gcttctcgat caccactttt tcgtcgcgcg ggctgaagat gaccacatcg ttgccctgac
     3361 tcaggacttc gcgtggtgaa ccatcgagga cgaacatgcg ggcatattcg ccttgcccgt
     3421 agttcatgtg agtgatctgg accgagcgtg cggtcttgcc cgactggtag atgaagaccc
     3481 cttggtagct aagttcgcgt gccgcctggg ccgctttctg caggaccatc caggggtctt
     3541 cgtcctgtgc tgcgttgacg taagaaaact gggtcgccag caggcaacct agcagcaaac
     3601 gccacattta tcgtttgctc tcgtaggcgg ctggctggat atagtaagcg gcactattgg
     3661 ctgagtaagc ctggtgcgcc aacaggtatt cggaagggat gctctgggcg atctcgatcg
     3721 gcgcagcatc ggctgcgttc tgggtctgct gttgcatgac catccagccg acgaacatca
     3781 ccgcggcgac ggaggccgcg accgaccaca tcatcgatga gcgcttgcgg aattgactcg
     3841 ctgactcaac caatgacggc tcgctgtcga gccgggccat cagccgatcc gtgaaatcgg
     3901 atcgcagata ggtctcgcca cgcatcgcgt cgccgatcag gtgataggtc gaccagcaat
     3961 cggccatctc cccacctgct ttgacagctg tcaacaaatg acccgagccc tcgacatcga
     4021 actcgtcatc catcagcgct gaaatttgat ctttcattac agtctcctga aacttaccat
     4081 ctcttgtctt gcggtgtgtc cagtaacgga cgcagcttct gggcgatggt ttcgcgtgcg
     4141 cggaagatcc gagagcgtac ggtgccgatc gggcaatcca tgagggttgc gatctcttcg
     4201 tagctcaggc cttcgatctc gcgcagcgtg atcgccgtgc gcaactcttc gggcaaggcc
     4261 gccaccgtat cgttcacggt tctggcgatc tccttgctca tcaactcggt ctctggcgta
     4321 tccatggtgc gcaactcgtt accgtcctcg aagttctctg catcttcgat ctcgatatcg
     4381 gtcgtcacct gtggcctacg gcccatggcc accagatagt tcttggcagt attgatgccg
     4441 atccggtaga gccaagtgta gaacgcgctc tcaccccgga aattcggcaa cgcacgatag
     4501 gccttgatga acgactcctg caccacgtcc tcgacttccg ccgggtcacg gatcaggcgt
     4561 gacagcaaac gtccgagctt gcgctggtac ttgtccacca gcatgccgaa ggcttttttg
     4621 tcaccgcgct gggcgcgttc aaccagttcc tggtctatct cacgattacc gaccatgctt
     4681 ttgtctctgt gcgcattcgc atctacagaa acgatactcc ctatctgcat tttctctatt
     4741 gtcggagtat aaccttgcag ggtcgtatca acgaaaccca tgcgcgaaaa ttcgtctagc
     4801 tttttcgtcg ttatcattta tcttcccttc agcctggtct tgactagctt ggtatacaag
     4861 acagcgctaa actcccgaaa gttcaagtat ccggacacat atgcaacatt acgacgtact
     4921 gatcatcggc agcggtctcg ccgggctcac cctggcgctg aaggtcgctg atcagaaacg
     4981 ggtctgcctg gtcagcaagc gcaccatcaa cgacagctcc agcaactggg cacagggggg
     5041 catcgccgcc gtgctggcag gcagcgattc gatcgaggcc catatccagg acaccctggt
     5101 tgccggcgcc gggctctgtg acgccgaagt cacccgcatg gtcgccgagc atgggcgcga
     5161 gaccgtggaa tggctagtga aggaaggcgt gccgttcacg cgcgaggcgg acgacagcgg
     5221 cttccatctc acgcgcgagg gcggtcacag ccatcgccgc atcatccatg ccgcagacgc
     5281 caccggccac gccgtgcaga agacgctggc ggaaaaggtg cgcgaacacg ccaacatcac
     5341 cttgctcgaa gatcacatcg cagtcgacct catcacgtcg cgcaaggtca agatcgaagg
     5401 caacgaatgc ctgggcgcct acgtgctcga caacgcttct ggcaaggtgc tgaccatcgg
     5461 cgccgaccac accgtgctcg ccaccggcgg cgccggcaag gtctacctgt acacgaccaa
     5521 ccccgacgtc agcactggcg atggcatcgc catggcctgg cgtgccggct gccgtgtcgc
     5581 caacatggag ttcatccagt tccatccgac ctgcctctat cacccgcagg ccaaatcgtt
     5641 cctcatttcg gaagcgctgc gtggcgaagg tggcgtcctc acgctgccgg acggcagccg
     5701 cttcatgcag gagcatgacg cgcgcggcga gctggcgccg cgtgatgtgg tggcacgcgc
     5761 catcgacttc gagatgaaaa agcgcggcat cgattgcgtc tacctcgaca tctcccatca
     5821 gccggctgat ttcatcctgt cgcacttccc caccatctac cggcggtgca tggagttcgg
     5881 catcgacatc accaaacaac cgatccctgt cgtaccggcc gcgcattaca gttgcggcgg
     5941 catcatgacc gaccatatcg gccgcaccga cctgccgcgg ctttatgcca tcggcgagac
     6001 cgcctgtacc ggcctgcatg gcgccaaccg gctggccagc aactctctac tcgaatgcct
     6061 ggtgttcggc caggccgctg cccgagatat cctcgcgcaa gcgcccaagc cccgggtcaa
     6121 gttaccttac tgggatgaga gtcgcgtcac cgacgccgac gaagagatca tcatcacgca
     6181 cacctggaac gaactgcgcc ggttcatgtg gaactatgtt ggaatcgtgc ggacgaccaa
     6241 gcgcctgatg cgggcattgc accgcatcca catgctgcgc gatgaagtcc acgagttcta
     6301 tagcaatttc cgcatcagca acaacctgat cgaactgcgg aacctgctgc aagtggcgga
     6361 actgatcgta ctgagcgcca tggagcgcca cgagagtcgg gggctgcact acagcaagga
     6421 ttatccggac atgctgccgg acgccatccc caccgtactc gaaccttcga actactacag
     6481 cctgctcgat agcgatcacg gccaccgcca acgcaaggcc gaacacgcgc gttagcgtgc
     6541 aggcaagcgc ctagacggtt gccaggcgaa tccctagtag cgcgccctgc cctttgaggt
     6601 catgcaggat ctgcgccccg aagccgacga tgacttcggg ctccgcatgg cccagctcag
     6661 tggcgatctg cagcaatgcc tgacgcgacg tcatcgcatc cgcctgccct gcgtgctgca
     6721 gcaagccaag caaacgcgcc gtgaccgggt tgagttcaat gaaacgcacc atatccgcag
     6781 catcgcggaa caccaggaaa tgcactggct cttccagcgg cgcttgcggc ttgaagcggg
     6841 gcgagatgcg atgcaccgga taatcgtagc gcaacaacgc tgaggctgcg gcgaacaccg
     6901 gcacccctgc cagcaggtcg ccgtgggtat cgacccgagt catgtcatcc tcgatgtcgg
     6961 cagaagcgat ggcgagctca atccattcgt aatgggtgag gctcatgagg tatggcggca
     7021 gatcctgccg cttctccaga tagctcagga aggcttgcgg gatctcccgg aacaaggggc
     7081 tagtcgcctg gtgcccggcg aagaagtcac gcaccagccg ggtccaggcg cgtttcccca
     7141 gcactttttg cgcgacggga aagcaggccg agacagcgcc atagagatta ttgaaaacga
     7201 tctcggtgta gacgcgcatg cgccgcgctg ccaccttgtc cggctttggc tggtgccgcg
     7261 gatcccggat atggctggtg aaacgcagct gatagcgctg gaacgccggc atcggatcat
     7321 ccgttcgcat cggctggata ctcatcgcgc ggcgctattc attagtacga tgctaatcat
     7381 taatgccatt cattgcaccg ctcttgccgc ttggcgatga ggcgcctgca gttcagcgat
     7441 gtaatcgatc tcgcggacca gcctcgtcag tggcgggatg ttggtatcgc gctcaagcaa
     7501 ggtcgggaac acgccgtata aatcataggc ttcccgcagc aacgcccaga ccgggtcgat
     7561 tacgtcctgg ccatgcgtgt cgatcgccag gtcatccg
//
LOCUS       DATMTW010000229         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_591338, whole genome shotgun sequence.
ACCESSION   DATMTW010000229 DATMTW010000000
VERSION     DATMTW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591338"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VN084_04530"
     CDS             <1..264
                     /locus_tag="VN084_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HWT28755.1"
                     /translation="VEQRWLPVLEGFQPQFVLISAGFDAHREDDMGGLALREADYAWV
                     TEQIMTVAARHASGRVVSVLEGGYALSALGRSASAHIKTLAGL"
     gene            complement(301..537)
                     /locus_tag="VN084_04535"
     CDS             complement(301..537)
                     /locus_tag="VN084_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HWT28756.1"
                     /translation="MTTRLPYRYRTIAFAAVMSLCTSMIVSGILISLHPRPGKLFIDV
                     WPSAFMTAWPVVFVAILVIAPLVNRLLDRIVEPS"
     gene            complement(534..1394)
                     /locus_tag="VN084_04540"
     CDS             complement(534..1394)
                     /locus_tag="VN084_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWT28757.1"
                     /translation="MRDLSRLGTTKELQLKDGRRLRYLQAGSGKPLLLLHTIRTQLDY
                     FQAVIPQLAQHYTVYALDLPGHGYASIDTQAQYDEPYMRSGVIGFIEQLDLTDLTIAG
                     ESIGAVLALTVSRHLPQRVARVIASNTYDYDTRYADGVRRGNFLANFVLFNFSIPING
                     AIFAALENRFLLGWILRGGLGKRRSMPEELVREFDRVGRRRGYRYVERNLLKNWRSWG
                     QARSLYPKQNTQVTLVYGEKDWSTVEERRRTADTLGLNGHITLKDTGHFAFVDNPEAL
                     VEIILDRQAT"
     gene            complement(1425..>1982)
                     /locus_tag="VN084_04545"
     CDS             complement(1425..>1982)
                     /locus_tag="VN084_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWT28758.1"
                     /translation="YDADRPETAVSVHDYLARTFGKLDILVNNAGIAREELMTNNSST
                     VTQEVLEQTFQTNLFAVIALTQTLLPLIRKSPAGRIVNLSSILGSLTLHSMAESPIAP
                     AKAFAYNASKTALNAYTVHLANELASTNIKVNSAHPGWVKTELGGEHAPMEVVDSNRT
                     SLYLATLAADGPNGGFFHEKDALPW"
BASE COUNT          361 a          550 c          657 g          414 t
ORIGIN      
        1 gtcgagcagc gctggttgcc ggtattggag gggtttcagc cacagtttgt gttgatctcg
       61 gcagggttcg acgcgcatcg tgaagacgac atgggtggcc ttgcgctgcg cgaggcggat
      121 tatgcctggg tcaccgagca gatcatgacg gtcgcggcgc gccatgccag tggccgtgtc
      181 gtttcggtgc tggaaggcgg ctatgctctg agcgcgctgg gtcgcagcgc cagtgcgcat
      241 atcaagacgc tggccgggct ctgatactgc cgggcccggg ccaagtccag cccagtcgcg
      301 ctaactcggc tcgacaatcc tgtccagcag gcggttcacc agcggcgcga tcaccaggat
      361 agctacgaac acgacaggcc acgctgtcat gaaggcggaa ggccagacat cgatgaacag
      421 cttgccaggc cgcgggtgca ggctgatgag gatgccggag acgatcatgc tggtgcatag
      481 cgacatgacg gcggcaaagg cgatggttcg ataacgatag gggagtcgtg tcgtcatgtc
      541 gcttgccggt cgaggatgat ctccaccagc gcttccggat tgtcgacgaa ggcgaagtgc
      601 ccggtgtcct tcaacgtgat gtgaccgttc aagcccaagg tatcggcggt gcgtcggcgt
      661 tcctcgaccg tcgaccagtc cttttccccg tagaccaggg tcacctgggt attctgcttg
      721 ggatacaggg aacgggcctg accccatgaa cgccagttct tcagcaggtt gcgctcgaca
      781 taacggtagc cccggcgacg gccgaccctg tcgaattccc tgaccagttc ctccggcatg
      841 gagcggcgct tgcccaagcc cccgcgcagg atccagccaa gcaggaacct gttctcaagc
      901 gcggcaaaga tggcgccgtt gatggggatg gaaaaattga acaacacgaa gttggcgagg
      961 aaattgccgc gtctgacgcc gtccgcatag cgcgtgtcgt agtcataggt gttgctggcg
     1021 atcacgcgtg cgacccgctg cggcaggtgg cggctcaccg tcagtgccag cacggcaccg
     1081 atggattcgc ccgcgatcgt caggtccgtc aggtccagtt gctcgatgaa gccgatgacg
     1141 ccgctgcgca tgtacggctc gtcatattgc gcctgggtgt cgatcgaagc gtagccatgg
     1201 ccaggcaggt cgagcgcata caccgtatag tgctgcgcca gctgcgggat gaccgcctgg
     1261 aaatagtcga gctgcgtacg gatggtgtgc agcagaagca gcggcttgcc gctgcctgcc
     1321 tgcagatagc gtaagcgtct gccgtccttc agttgcagct cttttgtcgt tcccaatctc
     1381 gataagtcgc gcattcttat tctcccttca tctgagttga gtgattacca aggcaacgcg
     1441 tctttttcat ggaagaaacc gccattgggg ccatctgcag ccagggtcgc gaggtaaagg
     1501 ctggtgcggt tgctgtcgac gacttccatg ggcgcatgct cgccgccgag ttcggtcttg
     1561 acccagcccg ggtgggctga attgaccttg atgttggtgc tggccagttc gttggcaagg
     1621 tgcacggtgt aggcgttgag cgcagtcttg gaagcgttat aggcaaaggc cttggcgggt
     1681 gcgatcggcg attcggccat gctatgcagc gtgagcgaac cgaggatgct ggacaggttg
     1741 acgatgcggc ctgccgggga tttcctgatt aagggtagca gggtctgggt gagggcgatg
     1801 acggcgaaga gattggtctg gaaggtctgc tccagcacct cctgcgtgac ggtgctgcta
     1861 ttgttggtca tcagttcttc cctggcgatg ccggcgttat tgaccaggat atccagcttg
     1921 ccgaacgtgc gtgccaagta gtcatgcacg ctcactgccg tttccgggcg gtccgcgtca
     1981 ta
//
LOCUS       DATMTW010000230         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_557552, whole genome shotgun sequence.
ACCESSION   DATMTW010000230 DATMTW010000000
VERSION     DATMTW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557552"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /locus_tag="VN084_04550"
     CDS             <1..>1517
                     /locus_tag="VN084_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28759.1"
                     /translation="IGNTAQAGRQLMHPDDREHAVYEVNNHLAGGSSVFISEFRLRCR
                     DGSWKWTLSRGMVVSRAIDGRPLRMIGTHADITSRKQSEQEIFRLAHYDPVTGLPNRI
                     LFLDRFAQEIKQAKRYGGQICLMYIDLDRFKEVNDSLGHDVGDLLLKEAGQRLQAIVR
                     STDTVARLGGDEFTILLSNIGDTGDIERLAQAVLDEMASPFKLNDETIYISTSIGISL
                     YPIDGEEVEVLLKNADQAMYEAKSQGRNRYYYFTSTMQEAAITRLRLANDLRAAIAEK
                     QFTVHYQPIVELASGAIRKAEALVRWQHPTRGMVSPVEFISIAEDTGLIVELGDFVFG
                     QASETVARWRRLQPDFQISINKSPVQFRQHRGTHTDWLSHLAAQGLAGNAIVIEITEG
                     LLFDANTAETQQLAAFHAAGVQIAIDDFGTGYSSLAYIKKFDIDYVKIDQSFTRNLQP
                     QSDELVLCEAIIVMSHKLGIKVIAEGVETPEQRDLLIAAGCDYAQGYLFSHPIPA"
BASE COUNT          330 a          488 c          431 g          268 t
ORIGIN      
        1 atcggcaaca cggcgcaagc cggccgtcag ctcatgcatc ccgacgatcg cgagcacgcg
       61 gtgtatgaag tgaataatca cctggccggc ggcagttcgg tgttcatatc cgagttccgc
      121 ctgcgctgca gagacggcag ctggaaatgg accctgagcc gtggcatggt ggtcagccgc
      181 gccatcgacg gacggccatt gcgcatgatc ggcacccacg ccgacatcac cagccgcaag
      241 cagtccgagc aggagatctt ccgcctggcg cattacgatc ccgtgactgg attgcccaac
      301 cgcatcctct ttctcgaccg tttcgcgcag gagatcaagc aggccaaacg ttatggcggc
      361 cagatctgcc tcatgtacat cgatctcgac cggttcaagg aagtcaacga cagcctgggc
      421 catgatgtcg gcgatctgct gttgaaagag gcggggcagc gcctgcaggc gatcgtgcgc
      481 tccaccgata ccgtcgcccg gctgggcggg gatgagttca ccatcttact cagcaatatc
      541 ggcgacaccg gtgatatcga gcgtctggcg caagcggtgc tggacgagat ggcatcgccg
      601 ttcaagctca atgacgaaac catctatatc agcaccagta tcggcatcag cctctacccg
      661 atcgatggtg aagaggtcga agtattgctg aagaatgccg accaagccat gtacgaagcc
      721 aagagccagg gccgcaaccg ctattactac ttcacctcga ccatgcagga ggccgccatc
      781 acccgcctgc ggcttgccaa cgacttgcgc gcagccatcg cagaaaagca gttcaccgtg
      841 cattaccagc ccatagtcga actggcgtcc ggcgccatcc gcaaggccga ggcgctggtg
      901 cgctggcagc acccgacgcg cgggatggta tcgccggtcg aattcatctc catcgccgaa
      961 gacacgggct tgatcgtcga actgggagat ttcgtctttg gccaggccag cgagacggtc
     1021 gcccgctggc ggcgtctgca accggatttc cagatcagca tcaacaaatc gccggtacag
     1081 ttccgccagc atcgcggcac ccataccgac tggctctcgc acctggccgc acaaggcctg
     1141 gccggcaatg ccatcgtcat cgagatcacc gaagggctgt tgttcgacgc caataccgcc
     1201 gaaacacagc agctggcagc gttccacgca gccggcgtgc agatcgccat cgacgatttc
     1261 ggcaccggct attcctcgct ggcctatatc aagaaattcg acatcgatta cgtcaagatc
     1321 gaccaatcct tcacgcgcaa cctgcagcca caatcggacg aactggtgct gtgcgaagcc
     1381 atcatcgtca tgtcgcataa gctgggcatc aaggtgatcg cggaaggcgt agaaaccccg
     1441 gagcagcgcg acctgctcat cgccgccggg tgcgactacg cgcagggtta cctgttctca
     1501 cacccgatcc ctgccga
//
LOCUS       DATMTW010000231         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_456976, whole genome shotgun sequence.
ACCESSION   DATMTW010000231 DATMTW010000000
VERSION     DATMTW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456976"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..467
                     /locus_tag="VN084_04555"
     CDS             114..467
                     /locus_tag="VN084_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28760.1"
                     /translation="MRTIALNALIAAVVSVATLTTYDRLIVQPRRTLGVVDSLAVFRL
                     EEQRLAGMMTRDMPPEQRERVATQARSFAQKFPLALEQLAIDCRCLVVERSAIVGTPP
                     HMVDLTPELRRRLSL"
     gene            464..985
                     /gene="traF"
                     /locus_tag="VN084_04560"
     CDS             464..985
                     /gene="traF"
                     /locus_tag="VN084_04560"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HWT28761.1"
                     /translation="MMRSYFAHIRHRWYIYLILTVIWMSALIRVFVNPTPWLPILFNV
                     TPSVPYWIVAIDYTARNPQRGDYIVYSFEGDAVRQFPGMRRQPLFKQVAGMPGDVVTV
                     TERHVYVNGRDVGVAKTHTITRLPLQPIVPTVIPPGYYYVAGQNPDSFDSRYAISGLV
                     ARRQILGRVTPLL"
     gene            1054..>1519
                     /locus_tag="VN084_04565"
     CDS             1054..>1519
                     /locus_tag="VN084_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HWT28762.1"
                     /translation="MLLCLLAIAPIVGLASDIGKLGPTYAISEQSLLGMIEERLRARA
                     ESGELARLMEKTAIAARGTVTSPTPVAGVTACIKARSFFFDPSVVLTENIVDASGNLI
                     FAAGTRKNPLDVVSLTRPLLFFDARDPRQRNKAQRMLKEHGQRVKLILTGGSY"
BASE COUNT          299 a          484 c          423 g          313 t
ORIGIN      
        1 ctgctgtttt cgaacaagct ggaggacaat aaaccgatcg atgaactccg cgcacaaggc
       61 ctttcggtcg acgaggcaat cgcccgcgtg ctcgaacaac ggggattgcg cgcatgagaa
      121 ccattgcact caatgcgctg attgcggccg tcgtcagcgt ggcaacgctg accacttacg
      181 accggctgat tgtccagccg cgccgcacgc tcggggtcgt cgactcgctc gccgtctttc
      241 ggcttgaaga gcaacgtctg gccggcatga tgacaagaga tatgcccccc gaacaacggg
      301 agcgcgttgc gacgcaggcg cggtcttttg cacagaaatt tcccctcgcg ttggaacagc
      361 tcgctatcga ttgccgctgc cttgtcgtcg agcgcagcgc catcgtcggc acgccgccgc
      421 acatggtgga cctgacgccc gagcttagaa ggagattatc gctatgatgc gttcctattt
      481 cgcccacatc cggcaccgtt ggtatatcta tttgatcctg acggtcattt ggatgtccgc
      541 cctgatccgg gttttcgtca accccacgcc atggctgcca attctattca acgtcacgcc
      601 cagtgtgccg tactggattg tcgccatcga ctacacagca cggaacccgc aacgtggcga
      661 ctatatcgtc tattccttcg agggcgatgc cgtgcggcag tttccgggca tgcgccgtca
      721 gccactgttt aagcaagtgg caggcatgcc gggcgatgtc gtaacggtca cagaacgtca
      781 cgtgtatgtc aacggccggg atgtgggagt cgccaagaca cacacgatca ctcgtttgcc
      841 tctgcagcca atcgtcccca cggtgattcc acccggctac tactacgtcg ccggccagaa
      901 tccggacagc tttgactcac gctatgcgat cagcggcctg gtggcacgac gccagatcct
      961 cggtcgggta acgcccttgt tgtgagaatc ctcatgtcaa cgactgcccc caacacttca
     1021 ctgccccctt tggcctcggc aagcgcatgt gcgatgctgc tctgtctact tgccatcgca
     1081 ccgatcgtcg ggttggccag cgacattggc aagctcgggc cgacgtacgc catctcggaa
     1141 caaagcctgc tcggaatgat tgaagaacgt ctccgggcgc gggccgagtc cggtgaactg
     1201 gcgcgactga tggaaaagac ggcaatcgct gcccgcggca ccgtcacctc gcccactccg
     1261 gtagccggcg tgaccgcttg catcaaggcg cgcagctttt tcttcgatcc gtcggtcgtc
     1321 ctgactgaaa acattgtcga tgcgagtggc aacctgatat tcgccgccgg cacgcgcaag
     1381 aatccgctcg atgtcgtgtc actaacgcgc ccgctgctgt tctttgatgc ccgtgatcca
     1441 cggcagcgca acaaggcaca gcgcatgctg aaggaacacg gacaacgcgt aaagctcatc
     1501 ttaaccggcg gctcttacc
//
LOCUS       DATMTW010000232         4925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_153995, whole genome shotgun sequence.
ACCESSION   DATMTW010000232 DATMTW010000000
VERSION     DATMTW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153995"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1125
                     /locus_tag="VN084_04570"
     CDS             238..1125
                     /locus_tag="VN084_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWT28763.1"
                     /translation="MNEPVTLLRIVSIVFPVFAIVLVGWAYGRGRVVDMSAPNQTNMD
                     IFVPALVFSALADRSFDLAGHQAFAIGAFCMIVGSGLIAWLIARLSGIPHKILVPTAM
                     FNNCGNLGLPLAVLAFGQEGLAAAVVMFMVSNLMHFSFGNWLLDHHAKWWNAWRYPVI
                     LAALAGLAVSFSHITVWEPLRIGIKMLGDVSIPLALFALGIRIAHSRVTAFGIGVTGT
                     LVRLGSGLLLTWLLANLLGLQGEQKAMLIIFGALPPAMINYVFAEKYGQGPDEVAAIV
                     LVGNVTAVLVIPVVLGLVL"
     gene            complement(1143..2528)
                     /locus_tag="VN084_04575"
     CDS             complement(1143..2528)
                     /locus_tag="VN084_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWT28764.1"
                     /translation="MTQLKALFNSTWHRITELYGRSFVWSLIVIAVSGILICALVFGF
                     INAAAPTKLTIASGPPGSSFERYAKKYQAILAKEGVKLTIVASNGSVDNLKRLADPKS
                     KVDVAFVQGGEAGNPAAGGTDSAAEKALAGEASDPNARLMSLGSIAYQPLFVFYRGEG
                     RHLLSEFKGQRIAIGQKDSGTHSLALELLKLNDIDSSNATLSTESAEQAVKALLAGEI
                     DAMFVMGDTASIALMRKLIQSPGINIFNFIQADGYARRVKYLNKLVLPQGSLDLGKNI
                     PVNDLYLVAPTVELIARDSLHPALSDLLLEAAREVHGSAGLFRKSGEFPRVIENDIRT
                     SPDATRYYTSGKSFLYSTFPFWLASLINRLLAVLVPIFLLLIPTMKVAPLIYRWRFQS
                     RIYPWYKVLLEIEQEAFAADLTDERRQRLIERLEKIEQSVNKIKIPPSFGDLLYGLKG
                     HIGFVRERLAG"
     gene            complement(2618..3961)
                     /locus_tag="VN084_04580"
     CDS             complement(2618..3961)
                     /locus_tag="VN084_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT28765.1"
                     /translation="MGRLFWKLFIGLSVTLVVVGAAIGGAVYLHEDRLNNDEGFLRDP
                     RSEFVVGLAANQLRYDDVRGVERLLAEWPGKRKPPEILIVDDGGPDVLGRAVPADALK
                     NALLALGQGKTDSVKRVVSPQGKSYVLFVPATNAKAGGPAYRPPPPEIQVLIALFASL
                     LFSAVFATYLSRPIRHLRKVSNRLAEGDLDARVSHLIGGRNDELSDLGQDFDFMAMRL
                     QTLLNAQRQLLHDVSHELRSPVARMRLAVGLAQQQPDKVAMALARIEREAERLDELLG
                     QMLTLARLESGLNNEHQEKVDIDELLDEVVRDVRFEAQAAGQRIDYQPRVKADIQGQR
                     ELLRRAFENVIRNAIKYGGSHGPVEITLDRGPGDRLTLTIRDHGPGIPDDALASVFQP
                     FFRLPGQEVRQANGYGLGLAIAKRAIDKHGGSIRLANASDGGLCVTIGFDHASMH"
     gene            complement(3965..4660)
                     /locus_tag="VN084_04585"
     CDS             complement(3965..4660)
                     /locus_tag="VN084_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWT28766.1"
                     /translation="MLQVLLIDDDIELCQMMAEYLEAEDFVLEVAHDGRQGATLAQSG
                     KFDLVVLDVMMPGLNGFDTLRLIRTQSQVPVIMLTAKGDDIDRIVGLELGADDYLTKP
                     FNPRELVARLHAIQRRVAVRDEPAAQRPLAPLRVGRIAIHPAERVIEWNGAVLEITTS
                     EYALIDVLVRNAGRVVSKADLFQQALGRPETRYDRSVDVHISNLRQKLGTLEDGRSPI
                     QTVRNIGYQFIPG"
BASE COUNT          875 a         1541 c         1543 g          966 t
ORIGIN      
        1 tccgcctggc tgtagaagac cgtgggcaat cgcgtgtagg aattatcgaa acgccagccc
       61 ggcctggagg tgttgagatc agacatacct gggattgtac ccctgcatcg cctgtcgttc
      121 ccaacctccg ggtgctgcgt actcagccgc tacgcgatgc gcaaggtccc ttctgtccga
      181 gcccttcttt tcatgcgcgc gattcgctac catgcgggaa agccacagga cccacccttg
      241 aacgaacccg tcacgctatt acgtatcgtc tccatcgtct tcccggtgtt cgccatcgtc
      301 ttggtcggct gggcctacgg gcgtggccgt gtggtcgaca tgagcgcgcc caaccagacc
      361 aacatggata tcttcgtccc ggcgctcgtg ttctcggcgc tggctgatcg cagcttcgac
      421 ctcgccggcc accaggcctt cgccatcggc gctttctgca tgatcgtcgg ttccggcctg
      481 atcgcctggc tgatcgcaag gttgagcggc attccgcaca aaatcctggt gccgaccgcc
      541 atgttcaaca actgcggcaa cctcggcctg ccactggcgg tactggcctt cggccaggaa
      601 ggcctcgccg ccgccgtggt catgttcatg gtgtccaacc tcatgcactt ctcgttcggc
      661 aactggttgc tcgaccacca tgccaaatgg tggaacgcct ggcgttatcc ggtgatcctg
      721 gcagcgctgg ccgggctggc tgtgagcttt tcgcacatca ccgtatggga gccgctacgc
      781 atcggcatca agatgctggg cgatgtctcg atcccgctgg ccctgttcgc cctgggtatc
      841 cgcatcgccc attcacgcgt caccgctttc ggcatcggcg tcaccggaac gctggtacgc
      901 ctaggcagcg gcttgttgct aacctggcta ctggcgaacc tgctgggcct gcaaggcgaa
      961 caaaaggcca tgctcatcat cttcggcgca ttgccgccgg ccatgatcaa ctacgtgttc
     1021 gcggaaaaat acggccaggg tccggatgaa gtcgccgcca tcgtactggt cggcaacgtc
     1081 accgcggtgt tggtgatccc ggtggtgctg gggctggtgc tttaagcgcc gggacgggcg
     1141 gcttaaccgg ccagccgttc ccgcacgaag ccgatatggc ctttcaggcc gtacaggagg
     1201 tcgccgaagg acggcgggat cttgatcttg ttgaccgatt gctcgatctt ctcgagacgc
     1261 tcgatcaggc gctgccggcg ttcatcagtg agatcggccg cgaacgcctc ctgctcgatt
     1321 tccagcagca ccttgtacca ggggtagatg cgcgactgga aacgccagcg gtagatcagc
     1381 ggcgcgacct tcatggtcgg gatcaacaac aggaagatgg gcaccagcac cgccagcagg
     1441 cggttgatga ggctcgccag ccagaacggg aaggtgctgt agaggaagct tttgcccgag
     1501 gtgtaatagc gcgtcgcgtc cgggctggtg cggatgtcat tctcgatcac gcgcgggaat
     1561 tcgccgctct tgcggaatag gccggcgcta ccgtgcactt cgcgcgcggc ttcgagcaag
     1621 aggtcagaca gcgcggggtg caggctgtcg cgggcgatca attcgacggt gggcgctacc
     1681 aggtagagat cattgaccgg gatgttcttg ccgaggtcga gcgaaccttg cggcagcacc
     1741 agcttgttga ggtatttgac gcgacgcgcg tagccatcgg cttggatgaa gttgaagatg
     1801 ttgatgcccg gcgactggat cagcttgcgc atgagcgcga tcgaagcggt atcgcccatg
     1861 acgaacatgg cgtcgatctc gccggctagc agcgccttga ctgcctgttc agccgattcg
     1921 gtactgagtg tggcgttgct gctgtcgata tcgttgagct tgagcagctc gagcgccagg
     1981 ctgtgggtgc cgctgtcttt ctggccgatg gcgatgcgtt ggcccttgaa ctcggacaac
     2041 agatgccgac cctcgccacg atagaacacg aacagcggct ggtaggcgat gctgcccagc
     2101 gacatcagcc gcgcgttagg gtcgctagct tcgcctgcca gggccttctc ggctgcgcta
     2161 tctgtgccgc cagctgccgg attgccggct tctccgccct gtacgaacgc cacatcgacc
     2221 ttgctcttgg gatcggcgag tcgcttgagg ttgtcgaccg agccattgga cgcgacgatg
     2281 gtgagcttga cgccttcctt ggccaggatc gcctggtatt tcttggcata gcgctcgaac
     2341 gagctgccgg gcgggccgct ggcgatggtc agcttggtcg gtgcggcggc gttgatgaag
     2401 ccgaacacca gcgcacagat gaggataccg ctcaccgcga tgacgatcag gctccagacg
     2461 aagctgcggc cgtagagctc ggtgatgcga tgccaggtgg aattgaacag ggctttgagt
     2521 tgcgtcatgt ctgcagtcta acattctccg atgcattccc cgagccggtc tgtatggctg
     2581 cgcctcgacc agcccatgag gctcggcggc ctcgcgatca gtgcatgctg gcgtgatcga
     2641 acccgatggt gacgcacaat ccgccatcgc tggcgttggc aagacggata ctgccgccgt
     2701 gcttgtcgat ggcgcgcttg gcgatggcaa gaccaaggcc atagccgttc gcctgccgaa
     2761 cctcttgtcc gggcaggcgg aagaacggct ggaacacgga agccagcgca tcgtccggga
     2821 tgcccgggcc atgatcgcga atcgtcagcg tcagccggtc gcctggccca cgatcgagcg
     2881 tgatctcgac cgggccgtgg ctgccgccat atttgatcgc gttacggatc acattctcga
     2941 aggcgcgccg cagcaactca cgctggccct ggatatcagc cttgacccgc ggctggtagt
     3001 cgatgcgctg accggcggcc tgggcctcga aacggacatc gcgcaccacc tcgtccagca
     3061 actcgtcgat gtcgaccttt tcctgatgtt cgttgttgag ccccgactcc agccgcgcaa
     3121 gcgtgagcat ttgcccgagc agctcgtcga ggcgctcggc ttcgcgctcg atacgcgcca
     3181 aggccatggc gaccttgtcc ggctgctgct gggccaggcc gacggcgagg cgcatgcgcg
     3241 ccaccggtga ccgcagctcg tgcgagacgt cgtgcagcag ctggcgctgt gcgttgagca
     3301 gggtctgcag ccgcatcgcc atgaaatcga aatcctgccc caggtccgac agttcatcgt
     3361 tacggccgcc gatcaggtgg ctgacgcgcg cgtcaagatc gccttcggcc aagcgattgc
     3421 tgaccttgcg caaatgccgg atcggccggc tcagatatgt ggcgaacacg gcgctgaaca
     3481 gcaggctggc gaacagcgcg atcagcacct ggatctccgg cggcggcggg cggtacgcag
     3541 gtccgccggc cttggcgttg gtggccggca cgaacagcac ataacttttc ccctgcggcg
     3601 aaaccacacg cttgacgctg tcggtcttgc cctgcccaag cgcgaggagc gcgttcttca
     3661 gggcatctgc cggcaccgcg cggccgagca cgtcagggcc gccgtcatcg acgatcagga
     3721 tctccggcgg cttgcgcttg cccggccatt cggcgagcag gcgctccacg ccgcgcacgt
     3781 cgtcatacct cagctggttg gcggcaaggc cgacgacgaa ctcgctacgc gggtcacgca
     3841 ggaaaccctc atcgttattg agacgatcct cgtgcagata gacggcaccg ccgatggccg
     3901 cgcctaccac aaccagcgta acggagagcc cgatgaacaa cttccagaaa agacggccca
     3961 tgcgctagcc cggtatgaac tgatagccga tgttgcgtac ggtctggatc ggcgagcgac
     4021 catcttccag cgtacccagc ttttgccgca ggttactgat gtgcacgtcc acgctacggt
     4081 cgtagcgggt ctccgggcgg cccagcgcct gctggaacag atcggccttg ctcactacgc
     4141 gaccggcgtt gcgcaccagc acgtcgatca gcgcatattc gctggtggtg atctccagca
     4201 cggcgccgtt ccattcgatc acgcgttcgg ccggatggat ggctatacgg cccacgcgca
     4261 acggcgccag cggacgctgg gcggcaggtt cgtcgcgcac ggccacccgg cgctggatcg
     4321 catgcagccg tgccaccagc tcgcgcgggt tgaagggttt ggtgaggtaa tcgtcagccc
     4381 ccagctcgag gccgacgatg cgatcgatgt cgtctccttt ggcggtcagc atgatgacgg
     4441 gcacctggct ttgcgtgcgg atcaggcgca aggtgtcgaa accgttgagg cccggcatca
     4501 tcacatccag caccaccaga tcgaacttgc cggactgggc gagcgtcgcc ccttgccggc
     4561 cgtcatgggc gacttccagg acaaaatcct cggcctccag atattcggcc atcatctggc
     4621 agagttcgat gtcgtcgtcg atgagcagta cttgcagcaa tgctgagtcc cttgtttatt
     4681 gagatggtac cggcgatcac ctgcgcgctg cggtccatat acacgcgctg cggtccatgt
     4741 gcatgcgcag caatgcatag tgctgattct gccaaagatt caaggcctga tgatgcgctc
     4801 cttacaaact tttacgtagt attacaccgc ctgaacgcaa gacaacatcc ggatatggag
     4861 aataggcgtt gaacagtctg cgacggaaca gccggccaga caatgcatcg gcaccgccgc
     4921 atgac
//
LOCUS       DATMTW010000233         6614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_625192, whole genome shotgun sequence.
ACCESSION   DATMTW010000233 DATMTW010000000
VERSION     DATMTW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625192"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VN084_04590"
     CDS             complement(<1..362)
                     /locus_tag="VN084_04590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWT28767.1"
                     /translation="MNMRVVVAGGGIVGCMTALELKNRGCEVTLVDRNVVASQTSGES
                     SWAGAGILFPLLPWMYADAVNQLTMAGAAIYPEFCEELLAETGIDPEYRRSGMLLLPT
                     FDMAQALQWSARQGIEAQ"
     gene            complement(367..2340)
                     /locus_tag="VN084_04595"
     CDS             complement(367..2340)
                     /locus_tag="VN084_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWT28768.1"
                     /translation="MQDTLPTILILLTSSVLVVALFRALNLPTMLAYFLVGLALGPET
                     SGILPDTEANRELAEFGIVFLMFSIGLEFSLPQLYSMRRVVLGLGGAQVVVTMLLAMG
                     VCMLFGLSWQTALVISGAFAMSSTAIVSKMLVERLDINSRHGRLAIGVLLFQDIAVVP
                     LLVMIPALAAQPEDLMATLGIAGLKAAAVLTLLFVFGKTLMNRWFSLVARQRSRELFV
                     LNVLMVTLILAFATKMAGLSYALGAFIAGMLISETHYRYQVESDIAAFRDILLGLFFI
                     TVGMLLDVHEVYEHAGLVLLVVTALLFGKSLIIAVLSRLFGYEPGVAVRAGVTLAQAG
                     EFSFVLLALGLDKGLISGPTLQIVLAGSLLSMVAAPFVIQQNLRIAEFFARSYTRNRK
                     HQIEGIEEVGKHLSDHVIICGFGRSGQYLARFLTEEKIPFIALDIDPTRVQEAAAAGE
                     RVMYGDATRRIVLRAAGLDRARALIISYNDVHGAMKILHVLRENHPELPVIVRTVDDT
                     NMEAFRAAGATEVVPEVLEGSLMLASHALVLLGVPLTRVIKRIRSFREEHYKMFRGFF
                     HGVTDAEQEALEKSQPRLHSVVIPASAYCIGKRVMDMGLEGYAVEVKTIRRNSFKPYD
                     AEPSSNFLEGDIVVLLGKPEALIRAEKLMLTGR"
     gene            2502..3515
                     /locus_tag="VN084_04600"
     CDS             2502..3515
                     /locus_tag="VN084_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HWT28769.1"
                     /translation="MGTPNPQTTSAPRAADAKQPLALAREVLEIEARAVEALAQRLDA
                     SFNRAVELILQCRGRVVVSGMGKSGHIGCKIAATLASTGTPSFFMHPAEASHGDLGMI
                     TRDDVVIALSNSGESDELLAILPLLKRMGAKLISITGNTRSSLAREADVHLDAHVDQE
                     ACPLGLSPTASTTAALALGDALAVTVLDMRGFSAEDFARSHPGGQLGRRLLLHVSDVM
                     RHGEAIPTVHLDAALSAGLLEMSRKGLGMTAVVDDDGRSVGIFTDGDLRRTFEQGINV
                     AGTTMRQVMHADPLTILPTQLAVDAVECMEKKKITSLLVVDQQGELVGALNMHDLLMA
                     KVV"
     gene            3516..4040
                     /locus_tag="VN084_04605"
     CDS             3516..4040
                     /locus_tag="VN084_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWT28770.1"
                     /translation="MTPVEQRAKRIKIVAFDVDGVMTNGGLMLSDEGLESKMFHSLDG
                     LGLKMLTATGIQIALVTARTSNVVVERAKNIKAKLVYQGAENKLVAFEQLLKDTGISA
                     EECAFMGDDVVDLPPLRRCGLAITVPHAMPLVKQYVHYTTERQAGFGAVREVCELIMK
                     AQGTFDGQMARFLE"
     gene            4061..4852
                     /gene="lptC"
                     /locus_tag="VN084_04610"
     CDS             4061..4852
                     /gene="lptC"
                     /locus_tag="VN084_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWT28771.1"
                     /translation="MLGRPTILFPLALLALLALLTLWIERSVQPPEHKLDGSNRHDPD
                     YILNYFTTTRTDVNGDLKYVLSAAEMKHYPDDDSTELKKPHFTQYSVDKPYTQIEGDR
                     GFVSSNGENVQFIGNVKVVRQAFKDRGEMTVTTTFLNIVPDKELATTDKAVVIRQAPE
                     TVIHAVGMIYNKKLKTLELHSRVRAHYVKPAPTGRKSNAPRPTYSKQPVKKPSASSAS
                     SEIVSPQSASKKQPSSKPDNQADRSVTKPANNSTRIRRTYEPASP"
     gene            4860..5381
                     /gene="lptA"
                     /locus_tag="VN084_04615"
     CDS             4860..5381
                     /gene="lptA"
                     /locus_tag="VN084_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HWT28772.1"
                     /translation="MTLLALAPPHAFAEKADRDKPIDLEADTVMVNDAKKTSTYTGNV
                     ILTQGTLVIKGDKLVVREDKDGFQHSTTYGNPQKPSTFKQKMEGKNEYMEGSGQRIEY
                     DGRMDKVQLFTKAWVKRGDDIVHGDYIMYDANSEYAEVIGGGSQAATAGTPTGRVRAV
                     IQPKKKPAPAEGQ"
     gene            5455..6177
                     /gene="lptB"
                     /locus_tag="VN084_04620"
     CDS             5455..6177
                     /gene="lptB"
                     /locus_tag="VN084_04620"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWT28773.1"
                     /translation="MSELIVEGLRKKYKSRTVVQDISLNLVSGEVVGLLGPNGAGKTT
                     SFYMIVGLVPLDDGRILLDGKDISRLPIHLRARMGLAYLPQEPSIFRKMTVEENILSI
                     LELQKFSPEQMKLQLEQLLQELHITHIRHNMAVSLSGGERRRVEIARCLATNPRFILL
                     DEPFAGIDPIAVLDIQKIIRFLTERNIGVLITDHNVRETLGICDRAYIVNEGRVFASG
                     APSEIIHNDSVREVYLGRDFRL"
     gene            6205..>6614
                     /locus_tag="VN084_04625"
     CDS             6205..>6614
                     /locus_tag="VN084_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWT28774.1"
                     /translation="MKQSLQLKFSQHLALTPQLQQSIRLLQLSTQELNQELESLLQAN
                     PLLERTDRPEDGGEAESFTPMPNGAETSVAAPAETPETEAPRQDEFGGGGEDYVEFSG
                     GSNRWDESSAGSDDDNDFTYQEAVSLTLREHLLG"
BASE COUNT         1453 a         2092 c         1962 g         1107 t
ORIGIN      
        1 tgttgcgctt cgatgccctg ccgcgcagac cactgcaagg cctgcgccat atcgaaagtc
       61 ggcaacaaca gcatgcccga gcgccggtat tccgggtcga tgccggtctc ggccagcaac
      121 tcctcgcaga attcggggta gatcgcggcg ccagccatgg tcagctggtt gaccgcatcg
      181 gcatacatcc acggcagcag ggggaacagg atcccggcgc ccgcccacga ggattcgccc
      241 gaggtctggc tggcgaccac attccggtcg accagcgtga cctcgcaacc gcgattcttg
      301 agttcgagcg cggtcatgca cccgacgatg ccgccaccgg cgaccacgac tcgcatgttc
      361 atcgcctcac ctgcccgtca gcatgagttt ttcggcccgg atcagggcct cgggcttgcc
      421 tagcagcacc acgatatcgc cttcgaggaa attggaactg ggttcggcgt catagggttt
      481 gaagctgttg cgccggatcg tcttcacttc cacggcatag ccttccagcc ccatgtccat
      541 gacgcgtttt ccgatacagt aggccgatgc cgggatcacc acagaatgca ggcgtggctg
      601 gctcttttcc agggcttcct gctcggcatc ggtgacgcca tggaagaagc cgcggaacat
      661 cttgtaatgc tcctcgcgga aactgcggat acgcttgatg acgcgcgtca gcggcacccc
      721 gagcagcacc agcgcgtgtg acgccagcat cagactaccc tcgaggacct cgggcacgac
      781 ctcggtcgcg ccggcggcac ggaaggcctc catgttggta tcgtccaccg tgcgcacgat
      841 cacaggcagc tccggatggt tctctcgcag cacgtgcagg atcttcatgg cgccatgcac
      901 atcgttgtag ctgatgatga gcgcacgtgc gcgatcgagc ccggccgccc gcaatacgat
      961 gcggcgggtg gcatcgccgt acatcacgcg ttcgccggca gccgctgctt cctgcacgcg
     1021 ggtcgggtcg atatcgaggg cgatgaaggg gatcttctct tcggtcagga aacgcgcgag
     1081 atactggccg cttcgcccga acccgcagat gatgacgtga tccgacagat gcttgccgac
     1141 ctcttcgatg ccttcgatct ggtgtttgcg gttgcgtgta tagctgcgcg cgaagaattc
     1201 tgcgatacgc aggttctgct ggatgacgaa cggcgccgcc accatcgaca gcaacgaccc
     1261 ggcgagcacg atctgcagcg tcggcccgct gatcaggccc ttgtccagac ccagcgccag
     1321 tagcacgaaa ctgaattcgc cggcttgcgc cagcgtcacg ccggcacgca cggcaacccc
     1381 gggctcatag ccgaacaagc ggctcaagac cgcgatgatc agactcttgc cgaacaacaa
     1441 tgcggtgacc acgagcagca ccagcccagc gtgctcgtag acttcatgca catcgagcag
     1501 catgccgacg gtgatgaaga acaggccgag caggatgtcg cggaacgcgg cgatatcgga
     1561 ttccacctgg tagcggtaat gcgtctcgga gatcagcatc ccggcgatga aggcgccaag
     1621 ggcataggag aggcccgcca tcttggtggc gaaggccagg atcagcgtca ccataaggac
     1681 gttgagcacg aagagctcgc gcgaacgctg acgtgcgacc aggctgaacc accggttcat
     1741 cagggtcttg ccgaagacga acagcagcgt cagtacggcg gcggccttga gaccggcgat
     1801 ccccagggtc gccatcaggt cctcgggctg cgctgccagc gccgggatca tcaccagcaa
     1861 aggcaccacg gcgatatcct ggaacaacag tacgccgatc gcgagccggc cgtgacgcga
     1921 gttgatgtcg agccgctcga ccagcatctt ggaaacgatg gcggtggacg acatggcgaa
     1981 ggcgccgctg atcaccagcg ccgtctgcca gctgagcccg aacaacatgc acacgcccat
     2041 cgccagcagc atggtgacga ccacctgcgc gccgccgagg cctagcacca cacgacgcat
     2101 ggaatagagt tgcggcaggc tgaattcgag gccgatgctg aacatgagga agacgatgcc
     2161 gaattccgcc agctcgcggt tggcttcggt gtccggcagg atgccggagg tctccgggcc
     2221 gagcgccagg ccgaccagga aataggccag catcgtcggc aggttgagtg cgcggaacag
     2281 ggctaccacc agtacggaac tggtcagcag gatcagtatg gtcggcagcg tatcttgcat
     2341 gagctgttca atattccttc atggacttgt gaccggagat ggtcgatatg tggcggcgcg
     2401 gcatgggtgt cggcaagccc ttggcatggc ccttagcaaa aatcgtgcta atttgctttc
     2461 atcaccttta ttcgcatgac attccctttt atacttgagc catgggaaca ccgaatccac
     2521 aaaccaccag cgcgccgcga gctgccgatg cgaagcaacc gctggctctg gcccgggagg
     2581 tacttgagat cgaggcgcgc gcggtcgaag cgcttgccca gcgactcgat gcaagcttca
     2641 accgcgctgt cgagctcatt ttacaatgcc gcgggcgcgt tgtggtgagc ggcatgggga
     2701 aatccggcca tatcggctgc aagatcgccg cgaccctcgc cagcaccggc accccatcct
     2761 tcttcatgca cccggccgaa gccagccacg gcgacctggg catgatcacg cgcgacgatg
     2821 tcgtcatcgc gctctccaac tccggcgaaa gcgacgagct gctcgccatc ctgcccttgc
     2881 tcaagcgcat gggcgcgaaa ctcatcagca tcaccggcaa cacgcgctca tcgctggcgc
     2941 gcgaggctga cgttcatctc gacgcccatg tcgaccagga agcctgcccg ctcggcctct
     3001 cgcctacggc cagcaccacc gcggccctgg cgctgggtga tgcgctggcg gtgacggtac
     3061 tcgacatgcg cggattctcc gccgaggatt tcgcccgttc gcatccgggc ggtcagctcg
     3121 gccgcagact gttgctgcat gtcagcgacg tcatgcgcca cggcgaggcc attcccaccg
     3181 tacacctcga tgcggcgctc tccgccggtc tgctcgagat gtcccgcaag ggcctgggca
     3241 tgactgcggt cgtcgacgat gacggccggt ccgtcggcat cttcaccgac ggcgatctgc
     3301 gacgtacctt cgaacagggc atcaatgtcg ctggcacgac catgcgacaa gtcatgcacg
     3361 cagacccgct gaccatcctg ccgacacagc tggcggtgga cgcggtggaa tgcatggaaa
     3421 agaaaaagat cacgagcctg ctggtcgtcg accagcaggg cgaattggtc ggcgcattga
     3481 acatgcacga cctgttgatg gcaaaggtcg tttgaatgac acccgtagaa cagcgcgcca
     3541 agcgcatcaa gatcgtagct ttcgacgtcg atggcgtcat gaccaatggc ggcctcatgc
     3601 tcagcgatga aggcctggaa tccaagatgt tccactcgct cgacggcctt gggctcaaga
     3661 tgctcaccgc cactggcatc cagatcgcgc tggtcacagc gcgcacctcg aacgtggtgg
     3721 tcgaacgcgc caagaacatc aaggccaagc tcgtatacca aggcgcggag aacaagctgg
     3781 tggctttcga acagctgctg aaggatacgg gcatcagcgc cgaggaatgt gcgttcatgg
     3841 gcgatgacgt cgtcgacctg ccgccgctgc gccgctgtgg cctcgcgatc accgtaccgc
     3901 acgccatgcc gctggtgaaa cagtacgtgc attacacgac ggaacgccag gccgggttcg
     3961 gcgctgtgcg cgaagtatgt gaactgatca tgaaggcgca ggggaccttc gatggccaga
     4021 tggcaagatt cctggaataa gcgagagggc tgcatcgtac atgctggggc gtccgaccat
     4081 cctcttcccg ttggcattgc tggcgctgct ggcgttattg accctgtgga tcgagcgtag
     4141 cgtgcaaccc ccggaacaca aactcgacgg cagcaaccgg catgacccgg attacatcct
     4201 caattacttc acgaccacgc gcaccgacgt caacggcgac ctcaaatacg tgctctcggc
     4261 ggctgaaatg aaacactatc cggatgacga cagcaccgag ctgaagaaac cgcatttcac
     4321 gcagtattcg gtggacaaac cctataccca gatcgaaggc gatcgcggtt tcgtctccag
     4381 caacggcgag aacgtgcagt tcatcggcaa cgtgaaagtc gtgcgccagg ccttcaagga
     4441 tcgcggcgag atgacggtga cgaccacctt cctcaacatc gtgccggaca aggaacttgc
     4501 taccacggac aaggcagtgg tgatccgcca ggcgccagag accgtgattc atgccgtcgg
     4561 catgatctac aacaagaagc tgaaaacgct ggagctgcac agccgggtac gcgcacacta
     4621 tgtcaaaccg gcgccgaccg gcaggaagtc caacgcgcca aggccgactt attccaagca
     4681 gccggtcaaa aagccgagtg cgtccagcgc cagctcggaa atcgtcagcc cgcaaagcgc
     4741 cagcaagaag cagccctctt caaaacccga taaccaggcc gaccgatccg tgaccaagcc
     4801 ggccaacaat agcacccgca tacggagaac ctatgaacct gcatcgcctt agcttgctca
     4861 tgaccctgct ggccctggcc ccgcctcacg cttttgcgga aaaggctgac cgggacaagc
     4921 ctatcgatct cgaggcggat acggtcatgg tcaacgacgc caagaagacc agcacctaca
     4981 ccggcaatgt catcctgacg cagggcacac tggtcatcaa gggcgacaag ctcgtcgtgc
     5041 gcgaggacaa ggacggcttc cagcacagca ccacctacgg caacccgcaa aagccctcga
     5101 ccttcaaaca gaagatggaa ggcaagaacg agtacatgga aggcagtggc cagcggatcg
     5161 aatatgacgg ccgcatggac aaggtacaac tgttcaccaa agcctgggtg aaacgcggcg
     5221 acgacatcgt ccacggcgat tacatcatgt atgacgccaa ctcggagtac gccgaagtca
     5281 tcggcggcgg ttcacaggcc gccaccgcag gcacgccgac cggacgtgtg cgcgctgtca
     5341 tccagccgaa gaagaaaccg gccccggccg aaggccagtg agctatctaa gcgtcaacga
     5401 atgcccggcc ccaatgaacc ctgaccgaat acggaagcaa gagcgaacca tccaatgagt
     5461 gaactgatcg tcgaaggact gcgcaagaag tacaaatcgc gcaccgtggt gcaggatatc
     5521 tcgctgaatc tggtgagcgg cgaagtcgtt ggcctgctcg gccccaacgg cgccggcaag
     5581 accaccagtt tttacatgat cgtcggcttg gtgccgctgg acgatggccg catcctgctc
     5641 gacggcaagg acatcagccg cctgccgatc catctgcgtg cacgcatggg gctcgcctac
     5701 ctgccacagg agccgtcgat cttccgcaag atgacggtgg aagagaacat cctctcgatc
     5761 ctcgaactgc agaagttctc gcccgaacag atgaagctac aactggagca gctgctacag
     5821 gaactgcaca tcacccatat ccgccacaac atggcagtga gcctctcggg cggcgagcgc
     5881 cgccgggtgg agatcgcccg ctgcctggcg accaatccgc gtttcatcct gctcgacgaa
     5941 ccgtttgccg gtatcgaccc gatcgcggtg ctcgatatcc agaagatcat ccgtttcctg
     6001 accgagcgca atatcggcgt gctcatcacc gaccacaatg taagagaaac cctcggcatc
     6061 tgcgatcggg cgtatattgt caatgaaggg cgcgtattcg cttccggcgc gcccagcgag
     6121 atcatccata acgacagcgt gagagaagtc tatctgggca gggatttccg cctctaaggg
     6181 cgcaaggcgg aacacagcaa gatcatgaag caaagtttac agctcaaatt ctcgcaacat
     6241 ctggcactga cgccgcagct gcagcagtcc atccgactgt tgcagctctc cacacaggaa
     6301 ctcaaccagg aactggaaag cctgctgcag gccaaccctc tgcttgagcg caccgacagg
     6361 ccggaagacg gcggcgaggc cgaaagcttc acgcccatgc ccaacggtgc cgagaccagc
     6421 gtcgcggcac cggctgaaac cccagagacg gaagcgccgc ggcaggatga gttcggtggc
     6481 ggtggcgagg actatgtcga attctccggc ggcagcaatc gctgggacga aagcagcgcc
     6541 ggtagtgacg acgataacga tttcacctac caggaagcgg tcagcctcac cctgcgcgaa
     6601 cacctgctcg gcca
//
LOCUS       DATMTW010000234         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_389349, whole genome shotgun sequence.
ACCESSION   DATMTW010000234 DATMTW010000000
VERSION     DATMTW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389349"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VN084_04630"
     CDS             complement(<1..1103)
                     /locus_tag="VN084_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWT28775.1"
                     /translation="MFGVAQAEEVMPEYTFLDSVKAGKSMTSFRLRYEYVDQDAKAEK
                     GNALTLRSLIGWQTAPFHNFSIGAQVIDVSKLENRYDDRALGDPEPGKGQHPIIADPD
                     NTDINQLYVDWTGIRNTKFRVGRQQVNLDNVRFVGDIGFRQVMQVFDGVSVINKSIPD
                     TEITVAHFDRVKQINTRLRSGDLDIVNAKYRISPSESVTAYGYFNNFDDLGFNAANGL
                     GAGADASSKTLGLRLDGAHGFTPEWKGLYTAEYAKQDEYSGGDDRIDAHYYKLGGGAM
                     YKSWFVRFDHETLSSNDGQYAFQTPFGTNHLFQGWADQFLVTPRQGIKDNFVTFGGKP
                     IGDLTLLAEYHVFDADENFAKFGGGFGDQYGKE"
     gene            complement(1235..>1787)
                     /locus_tag="VN084_04635"
     CDS             complement(1235..>1787)
                     /locus_tag="VN084_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT28776.1"
                     /translation="AVVFQNHSLLPWLTCTENVYLAVERVFSKSEDKQQMLARTAAAL
                     ELVGLSHAADKRPGEISGGMKQRVGIARALAMEPKVLLLDEPFGALDALTRANLQDEL
                     MRIMARSGCTTVMVTHDVDEAVLLSDRIVMMTNGPGATVGEILNVDLERPRARLALAE
                     DPHYNRLRGEVLRFLYERHAKAA"
BASE COUNT          331 a          517 c          565 g          374 t
ORIGIN      
        1 aattccttgc cgtactggtc gccgaaaccg ccgccgaact tggcgaagtt ttcgtcggca
       61 tcgaacacgt ggtattcggc gagcagggtc aggtcgccga tgggcttgcc gccgaaagtc
      121 acgaagttgt ccttgatgcc ctgacgggga gtgaccagaa actggtctgc ccagccctga
      181 aacaggtgat tggtaccgaa aggagtctga aaggcgtatt ggccatcatt actggacagc
      241 gtctcgtgat cgaagcgcac gaaccagctc ttgtacatgg cgccaccgcc aagcttgtaa
      301 taatgggcgt cgatacggtc atcaccgccc gagtactcgt cctgcttggc atattcggcg
      361 gtatagaggc ctttccattc cggcgtgaag ccgtgggcac catcaaggcg caatcccagg
      421 gtcttgcttg aggcatccgc gccggcgccg aggccgttgg cggcgttgaa tcccaagtcg
      481 tcgaagttgt tgaaataacc gtaagcggtg accgactcgg agggcgagat gcggtacttg
      541 gcattgacga tatcgaggtc gcccgagcgc agccgggtgt tgatctgctt cacgcggtcg
      601 aaatgcgcga ccgtgatctc ggtgtcaggg atgctcttgt tgatgaccga gacgccgtcg
      661 aacacctgca tgacctggcg gaagccgata tcaccgacga agcgcacgtt atcgagattg
      721 acctgctggc gaccgacccg gaatttggtg ttgcggatgc cggtccagtc gacatagagc
      781 tggttgatat cggtgttgtc cggatcggcg atgatcgggt gctggccctt gcccggctcg
      841 gggtcgccca gcgcgcgatc gtcgtagcgg ttctcgagct tgctgacgtc gatgacctgc
      901 gcgccgatgc tgaaattatg gaaaggcgcg gtctgccagc cgatcaggct gcgcaaggtc
      961 aaggcattgc ccttctccgc cttggcgtcc tggtcgacat attcataacg caggcggaag
     1021 ctggtcatgc tcttgccggc cttcaccgag tcaaggaagg tatattccgg catgacctcc
     1081 tcggcctggg cgacaccgaa catgctcgcc agcagtgcac aggcgagggc ttgacgcatc
     1141 gggttcatgg gattactttt cacgttgttg ctccttaagt aagagtgaca tttggtttta
     1201 agcccgctct aaaccggcgg gttttttttc gtgctcaggc tgctttggca tgacgttcgt
     1261 aaaggaagcg cagcacttcc ccacgcagac ggttgtaatg cgggtcttcg gccagtgcca
     1321 gtcgcgcgcg aggccgctcc agatcgacat tgaggatctc gccgacggta gcgcccgggc
     1381 cgttggtcat catgacgata cggtcggaca gcagcacggc ttcgtcgacg tcgtgcgtca
     1441 ccatcaccgt ggtgcagccg gatcgcgcca tgatgcgcat gagctcgtcc tgcaggttgg
     1501 cgcgggtcag tgcgtcgagc gcaccgaacg gctcatccag cagcagcacc ttgggctcca
     1561 tcgccagcgc acgggcgatg ccgacgcgct gtttcatgcc gccggagatc tcgcccggcc
     1621 gcttgtcggc ggcgtgcgag aggccgacaa gctcgagcgc agccgctgtc ctggcaagca
     1681 tctgctgctt atcctcggat ttgctgaaca cgcgctcgac agccaggtag acgttctcgg
     1741 tacaggtcag ccacggcagc agcgagtggt tctggaacac cacggcg
//
LOCUS       DATMTW010000235         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_423432, whole genome shotgun sequence.
ACCESSION   DATMTW010000235 DATMTW010000000
VERSION     DATMTW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423432"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2114
                     /gene="ppsA"
                     /locus_tag="VN084_04640"
                     /pseudo
     CDS             1..2114
                     /gene="ppsA"
                     /locus_tag="VN084_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985154.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
BASE COUNT          473 a          694 c          629 g          403 t
ORIGIN      
        1 gccagtggat catggaccaa cccttcacgc cggaactgga aaaagggatc gccgagcact
       61 accagcgcgt caccgccgat atgggcgacc acgtcagctt tgccgtacga tcttccgcca
      121 ctgccgagga cttgccggac gcatagtttg caggacagca agagtctttc ttgaatatca
      181 agggcttgga aaatattgtt catgcgatta aagaggtttt tgcatcgctg tataacgatc
      241 gcgccatctc ctaccgcgtc cacaagggct tcgtccatgc cgacgtcgcc ttgtctgccg
      301 gcgtgcagcg catgtgccgc agcgacatcg gcagctccgg cgtcatgttt accatcgaca
      361 ccgaatcggg cttccgcgac gtagctttca tcaccgcatc ctatggcctc ggcgagaccg
      421 tggtacaggg tgcggtcaat ccggacgagt tctacgtgca caagccactg ctcaaccagg
      481 gcaagccagc catcgtgcgc cgtacgcttg gctccaagct gatcaagatg gtgttcagca
      541 atgccaatga agccggcaag agcacgcaca cgatcgataa caccaaggaa gacagcagcc
      601 gctactcgct ggacgacagc gaagtgctgg aactggcccg tttcgcagtc atcatcgagc
      661 agcactatgg tcatccgatg gatatcgaat ggggcaagga tggcactgac ggtaagctgt
      721 acatcctgca ggcacgtccg gaaacggtga agtcacagga aaaggctggc agcgcagtcg
      781 agaagttcgc tctcaagggc aagggcgagg cgatcgtcac cggccgtgcc gtgacccaga
      841 agatcggtgt cggtccggtg cgggtcgtca agcatgcctc cgagatgaac accgtacagc
      901 ctggcgacgt acttgtcgcc gacatgaccg acccgaactg ggaaccggtc atgaagcgcg
      961 cctccgcgct cgtcaccaat cgtggcggtc gtacctgcca cgccgcgatc atcgcgcgtg
     1021 aactcggtat cccagccatc gtcggcagcg gcaacgccac cgaagtcctg accgaaggcg
     1081 ccatcgtcac cgtctcctgt gctgaaggcg aaaccggtta cgtctaccag ggcgcgctgg
     1141 aatacagcgt gaccaaggaa gccgaggccg agctgaccaa gccgccggtc aagatcatga
     1201 tgaacgtcgg caatccggat atggccttca atttcgccca gatcccgaac gacggcgtcg
     1261 gcctcgctcg cctggagttc gtcatcaaca acatgatcgg cattcacccc aaggccatcc
     1321 tcaacgcatc gcgcctgccg actgcgatgc agcaggaagt catgtcgcgc tctgcaggct
     1381 acgccaaccc gcgccagttc tacatcgaca agctggcaga aggtgttgcc accatcggtg
     1441 ccgcgttctg gcccaagccc gtcatcgccc gtatgtccga cttcaagtcc aacgaatatc
     1501 gcaagctgct gggtggcgag atctacgaac cggaagaaga gaacccgatg ctcggcttcc
     1561 gcggcgcggc acgctacatc gcagacgatt tccgtgattg cttcgagatg gaatgcctcg
     1621 ccatgcgcaa ggtccgcgat gagatcgggc tgaccaacat cgagctcatg atccccttcg
     1681 tgcgcacgct gagcgaagcc cgcgccgtca tcgatatcct cgcgagctac ggcctcaagc
     1741 gcggcgagaa cgggctgcgt ctgatcatga tgtgcgagat cccgtccaac gcgctgcttg
     1801 ccgaggagtt cctcgaatat ttcgacggct tctccatcgg ctccaatgac ctcacgcaat
     1861 tgtcgctggg catggaccgt gattccggcc tcatggccca tgggttcgac gagcgcgacc
     1921 cggcggtcaa ggtgctgctc aacatggcga tcagtacggc taaccgcctc gacaagtacg
     1981 tcggtatctg cggtcagggc ccgtcggatc atccggactt cgccgaatgg ctggtcaagg
     2041 ctggcatcaa gtcggtatcg ctcaacccgg ataccgtggt caagacctgg caacgcctga
     2101 ccaagctgag ctaacggacc cgaccctaac ccatgcatca ccgtacggta ttcttcattt
     2161 cggacggcac tggcctgacc gcgcaagcgc tcggccagc
//
LOCUS       DATMTW010000236         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_122773, whole genome shotgun sequence.
ACCESSION   DATMTW010000236 DATMTW010000000
VERSION     DATMTW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122773"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VN084_04645"
     CDS             complement(<1..1521)
                     /locus_tag="VN084_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HWT28777.1"
                     /translation="MTSAPPKLYLNLYWHMHQPDYRDTLTGEYVLPWTYLHAIKDYTD
                     MAHHIEQNPQAKVTFNFVPVLLDQLEDYAAQFKSGDIRDPLLRLLSLENLDDISEEER
                     CLIIDSCFKSHHDKMLAPFAHYQSLHDIYRGLEHQGKTAFNYLSGQYMADLLVWYHLA
                     WTGESVRRSDKLVQALMQKGSQFDTKDRRGLFTLIGTLISDLIPRYRKLQQAGRIELS
                     STPHFHPILPLLLDFKCTRDAMPYAPLPRCAQYPGGRLRARAHIEAAQASHKARFGEL
                     PHGMWPAEGGVSHAALSLMAEHGLQWAATGEGVLANSLYKSFDSSDLPPRAEYLYRPY
                     RVTDGNHDIQCFFRDDNLSDKIGFEYSKFHAGDAVNDFIAALEAIHADNASPQSPVVS
                     VILDGENAWEYYPYNGYYFLSELYAALAAHPDIEMTTFSEIVEKTRKGDEVELRTLPA
                     VAAGSWVYGSFSTWIGSTDKNLGWDLLCEAKKNYDRAIQAGSLSKREQEACERQLAI"
     gene            complement(1570..>2531)
                     /locus_tag="VN084_04650"
     CDS             complement(1570..>2531)
                     /locus_tag="VN084_04650"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWT28778.1"
                     /translation="EQWYKGTADAVFQNLDILRSGGAEYVLILAGDHIYKMDYGQMLA
                     SHVRNKADMTIACINVPLEDAKAFGVIGVDESDRVTEFEEKPANPKTIPGNPEQAFAS
                     MGIYVFNSSFLYEQLIRDADDHTSTHDFGKDIIPRMIRKYRVYAHRFTDSCVGAADDN
                     YYWRDVGTVDAYWEANMELTKVVPELNLYDRNWPIWTYQEQLPPAKFVFNNDDRRGSA
                     TDSLISGGCIISGSQVTSSVLFSDVRVNSYSTIEDSVILPNVDIGRYVTLKRVVVDKG
                     ARIPDGMEIGLNPEQDRKRFHVSEKGITLVTPDMLGQSLHHAR"
BASE COUNT          504 a          726 c          739 g          562 t
ORIGIN      
        1 gatcgccagc tggcgttcgc aggcctcttg ctcacgcttg ctcaggctgc cagcctggat
       61 cgcacggtca tagttcttct tcgcttcaca cagcagatcc cagcccaggt ttttgtcggt
      121 gctgccgatc catgtgctga aactgccgta gacccagctg ccggccgcca ccgccggcag
      181 tgtcctcagt tcgacctcgt cgcccttgcg ggtcttttcg acgatctcgc tgaaggtggt
      241 catctcgata tccgggtgcg ccgccaaagc cgcataaagt tcgctcaaaa agtaatatcc
      301 gttgtacgga tagtattccc aggcgttctc gccatcgagg atgacactca ccacagggct
      361 ctgtggactg gcattgtcgg cgtggatggc ttcgagcgct gcgatgaagt cgttgacggc
      421 atcgccggcg tggaatttgg agtattcgaa cccgatcttg tccgataggt tgtcgtcacg
      481 gaaaaaacac tggatatcgt gattgccgtc tgtcacccgg tagggccggt agagatattc
      541 cgcacgcggc ggcaggtctg agctgtcgaa ggatttgtac aggctgttgg ccagcacacc
      601 ttcgcctgtc gctgcccact gcagaccatg ttcggccatc agcgacaatg ccgcatgtga
      661 gaccccgcct tcggctggcc acatgccatg cggcaattca ccgaaccggg ccttgtggga
      721 ggcctgcgcg gcttcgatgt gcgcacgcgc gcgcaatcgg ccgcccggat attgtgcgca
      781 gcgcggtaat ggcgcgtaag gcatggcatc acgcgtgcac ttgaaatcga gcagcaaagg
      841 caggatcggg tggaaatgcg gcgtactcga aagctcgata cggcctgcct gttgcagctt
      901 cctgtaacgc gggatgaggt cagagatcag cgttccgatc agcgtgaaca gcccccgcct
      961 atccttggtg tcgaactggc tgcccttctg catcaatgcc tgcaccagct tatcgctgcg
     1021 gcgcacgctt tcgcctgtcc aagccaggtg ataccacacc agcaggtcgg ccatgtactg
     1081 gcctgagagg taattgaatg cggtcttgcc ctgatgttcg aggccacgat agatatcgtg
     1141 caggctctgg tagtgcgcga acggcgcgag catcttgtcg tgatggctct tgaagcagct
     1201 gtcgatgatg aggcagcgct cttcttcgct gatgtcatcg agattctcca gcgacagcag
     1261 tcggagcaag gggtcacgga tatcgcccga cttgaactgt gcggcataat cctccagttg
     1321 atccagcaac accgggacga aattgaacgt gaccttggcc tggggattct gctcgatgtg
     1381 atgcgccata tcggtgtaat ccttgatggc gtggaggtac gtccagggca ggacatattc
     1441 gccggtcagc gtgtcgcggt aatccggctg gtgcatgtgc cagtagagat tgagatacaa
     1501 cttgggtggc gccgatgtca tgacaaaacc ttctttaata aacggatgac ccggttgcca
     1561 cgggccatac tatctggcgt ggtgtagcga ctgccccagc atatcgggcg tcaccagcgt
     1621 gatgcctttt tcggaaacat ggaagcgctt gcgatcctgt tccggattga gaccgatctc
     1681 catgccatcg gggatacgtg cgcccttgtc caccacgacc cgcttcaacg tcacatagcg
     1741 gccgatgtcc acgttcggca ggatcacgga atcctcgatc gtgctgtagc tgttgacccg
     1801 cacatcggag aacagcaccg aactcgtgac ctgcgaaccg ctgatgatgc atccgccaga
     1861 aatcagcgaa tccgtggccg aaccgcgccg atcatcgtta ttgaacacga acttggctgg
     1921 tggaagctgc tcctgatacg tccagatcgg ccagttgcgg tcatacagat tgagctcagg
     1981 caccaccttg gtcaattcca tgttcgcttc ccagtaagca tctaccgtac caacatcgcg
     2041 ccagtaatag ttatcgtcag cggccccgac acagctatcg gtgaaacggt gcgcatagac
     2101 acggtacttg cggatcatgc gcgggatgat gtccttgccg aaatcatgcg tggaagtatg
     2161 gtcgtccgcg tcgcggatca gctgctcgta aaggaaggac gagttaaaga cgtagatgcc
     2221 catgctggcg aaggcctgtt ccggattacc gggaatggtc ttggggttgg ccggcttttc
     2281 ctcgaattcg gtgacgcggt cgctctcgtc aacgccgatc acaccgaagg ccttggcgtc
     2341 ttccagcggc acattgatgc aagcgatggt catgtcggcc ttgttgcgga catgcgaagc
     2401 cagcatctgg ccgtaatcca tcttgtaaat gtggtcgccg gccaggatca gcacgtactc
     2461 ggcaccgccg ctgcgcaata tgtccaggtt ctggaacacg gcatccgccg tgcctttgta
     2521 ccattgctcg a
//
LOCUS       DATMTW010000237         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_154419, whole genome shotgun sequence.
ACCESSION   DATMTW010000237 DATMTW010000000
VERSION     DATMTW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154419"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..445)
                     /locus_tag="VN084_04655"
     CDS             complement(176..445)
                     /locus_tag="VN084_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWT28779.1"
                     /translation="MKTVIVCVNARSNPNQPSCARRGSLKLLARLTAEIEKRQLPIRV
                     DSFHCLGLCAHGPNAKLAPDGPTLQQLDTEDLSALMAEIEAFIAK"
     gene            complement(442..1212)
                     /locus_tag="VN084_04660"
     CDS             complement(442..1212)
                     /locus_tag="VN084_04660"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWT28780.1"
                     /translation="MSVHGSATDPHSLFVSDLHLWPSRPAITALFIDFLAQRACRAEA
                     LYILGDLFEYWAGDDDLEEAHHRSVIDALAACAASGTRIYFMHGNRDFLIARRFAEAA
                     QATLLSDPTLLVLYGQRTLLSHGDAYCTDDIAYQDFRRQVREPSWQAQFLSQPLAARK
                     AQIEAIRQRSEQEKSQKAEDIMDVNPAAITQLLREHHYPPLFIHGHTHRPAMHRHMVD
                     GHLCQRWVLGDWYDQGSCLRVDASGCRNIALSPMPPAP"
     gene            complement(1220..1711)
                     /locus_tag="VN084_04665"
     CDS             complement(1220..1711)
                     /locus_tag="VN084_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWT28781.1"
                     /translation="MIKLHTNHGEITIELDAEKAPLTAANFLQYVEEGFYENTIFHRV
                     IDGFMIQGGGFDTDMRQKATKAPIKNEADNGLANKRYTIAMARTPNPDSASSQFFINV
                     ADNDFLNFSAPTAQGWGYCVFGKVTAGTEVVDKIRQVKTGNRNGHQDVPVEPVIIERA
                     EVC"
     gene            complement(1704..2399)
                     /locus_tag="VN084_04670"
     CDS             complement(1704..2399)
                     /locus_tag="VN084_04670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWT28782.1"
                     /translation="MMRIRPFFLALLTLCFSVFGLQASAADLHRANPQVEFVTNEGSF
                     IVELYPDKAPQTVANFMQYVSSGYYQGTTFHRTIERFMIQGGGMDENLAEKPTLAPIP
                     NESNNGLRNEPGTLAMARAYAPDSATSQFFINMTDNKFLNYYKPDPAYVGYAVFGRVI
                     RGMDTVERISKSPTHKMGMHQNVPIEPIVIERVAVLDQPVIADASASLPAGKVSEKAA
                     TPKKSSTKGKKRD"
     gene            complement(2420..>3204)
                     /locus_tag="VN084_04675"
     CDS             complement(2420..>3204)
                     /locus_tag="VN084_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWT28783.1"
                     /translation="IVADTSNSRLYVYRNDNGHPQYVADYYVTVGKNGSEKQTEGDKR
                     TPVGVYVAGSKLTRKLPDFYGDAAYPLNYPNEWDRKQGRKGHGIWLHGTPSDTYSRPP
                     RASDGCVVIANPDLKSLSAILQNGNTPVIITNHLQWLDHEQPSANRQALAAALEDWRK
                     DWESQDSERYLAHYADRFFTRTTDLAGWAKEKRRIQASKMDVEIRLSNISMFRYPAND
                     TAMAVVTFDQDYKSNHLDSRMRKRQYWIQEDNRWKILYEGSV"
BASE COUNT          569 a          890 c         1015 g          730 t
ORIGIN      
        1 ctttgcccat ccgccctatc gggcgatcgt cacgccggtg ctctaccttg aagtgttctt
       61 catccttatc aatggcggac tgatgctgct ggctttcagg gagcatgcgt tcgccccgga
      121 tacccagcaa ccgggcaagg cttacgccgc ccgcctcaag tccggcaagc actgatcatt
      181 tcgcgataaa ggcttcaatc tcggccatca gtgccgacag gtcttccgta tcgagctgct
      241 gcagtgtggg gccatccggc gccagcttgg cgttcgggcc gtgggcgcag agtccgaggc
      301 aatggaagct atcgacgcgt atcggtaact ggcgtttttc gatctctgcg gtgagtcgcg
      361 ccagcaactt aaggctgccg cgcctggcgc aggagggctg gttgggattg ctgcgcgcat
      421 tgacgcagac gatcacggtt ttcatggcgc cggcggcatg ggcgataacg cgatattgcg
      481 gcagccggaa gcgtccaccc gcaagcagct tccctggtca taccagtcac ccagaaccca
      541 ccgctggcag agatgcccgt cgaccatatg ccgatgcatg gccggccgat gtgtatggcc
      601 atggatgaac agcggcggat agtggtgttc gcgcagcagc tgggtgatgg cggctgggtt
      661 cacatccatg atgtcttccg ctttttggga tttttcctgt tcgctacgtt ggcggatggc
      721 ttcgatctgc gctttgcgcg cggccagggg ctgcgacagg aactgcgcct gccaagacgg
      781 ctcgcgcacc tgccggcgaa aatcctggta agcgatatcg tcggtgcagt aagcgtcgcc
      841 atggctgagc aaggtgcgct ggccatagag cactagcagc gtcgggtctg acagcagcgt
      901 cgcctgtgcg gcctcggcga atcggcgtgc gatcagaaaa tcccgattgc catgcatgaa
      961 ataaatgcgc gtgccagatg ccgcgcaggc ggcgagcgca tcgatcacgc tacggtggtg
     1021 cgcctcctcg aggtcgtcat caccggccca gtattcgaac agatctccga ggatatacag
     1081 ggcttcggcc cggcaagccc tttgcgccag aaagtcgatg aacaatgccg tgatggctgg
     1141 ccgtgagggc caaaggtgca gatcggaaac gaacaggctg tgcgggtctg tcgcgcttcc
     1201 gtggacggac atgatatccc tagcagactt ctgcgcgttc gatgatcacc ggctcgacgg
     1261 ggacatcctg atggccgtta cgattacctg tcttgacctg ccggatcttg tcgacgacct
     1321 cggtgccagc ggtgaccttg ccgaagacgc agtagcccca gccttgtgcg gtgggggcgc
     1381 tgaagttgag gaaatcgtta tcggcgacat tgatgaagaa ctggctgctg gccgaatccg
     1441 ggttcggcgt gcgggccatg gcgatggtat agcgcttgtt ggcgaggcca ttgtcggctt
     1501 cgttcttgat cggtgccttg gtggccttct ggcgcatgtc ggtatcgaat ccgccaccct
     1561 ggatcatgaa accgtcgatc acgcgatgga agatggtgtt ctcgtaaaag ccttcttcta
     1621 cgtattgcag aaaattggct gccgtcaacg gtgccttttc ggcgtccagt tcgatggtta
     1681 tctcgccatg gttggtatgg agtttaatca cgcttttttc ctttggtgga tgatttcttg
     1741 ggggtggcgg ccttctcgct caccttgccc gcaggcaggc tggcgctcgc gtcagcgatc
     1801 acaggctgat ccagcacggc gacccgttcg atgacgatcg gctcgatcgg tacattctgg
     1861 tgcatgccca tcttgtgggt cggcgacttg ctgatgcgtt cgaccgtgtc catgccacgg
     1921 atcacccggc cgaacacggc atagccgacg taagccggat caggcttgta gtagttgagg
     1981 aacttgttgt cggtcatgtt gatgaagaac tgggaggtcg ccgaatccgg agcataggcg
     2041 cgggccatgg ccagcgtacc cggctcgttc ctgaggccgt tgttggattc gttcgggatc
     2101 ggtgccagcg tcggtttctc cgccaggttc tcgtccatgc cgcccccctg gatcatgaag
     2161 cgttcgatcg tgcgatggaa cgtggtgccc tgatagtaac cactgctcac gtattgcatg
     2221 aaattggcga cggtctgcgg tgccttgtca ggatagagct cgacgatgaa gctgccctca
     2281 ttggtcacga actcgacctg cggattcgct cggtgcaggt cggcggctga tgcctgaagg
     2341 ccgaaaacgc tgaaacagag ggtgagcaat gccaggaaaa aaggacggat acgcatcatg
     2401 atgaacctcc cagagcaggt cagacagaac cttcgtacaa aattttccag cgattgtctt
     2461 cctggatcca gtattgccgc ttgcgcatgc ggctatccag gtggttgctc ttgtaatcct
     2521 ggtcgaaggt gaccacggcc atcgctgtgt cattcgcagg atagcggaac atgctgatgt
     2581 tcgacaaccg gatttccaca tccatcttgc tggcctggat gcggcgcttt tccttggccc
     2641 agccggccag atccgtcgtg cgggtgaaga agcgatcggc gtagtgcgcg aggtagcgct
     2701 cactgtcctg cgattcccag tccttgcgcc agtcttccag cgcggccgcc agggcctgcc
     2761 ggttcgcgct gggttgctcg tgatccagcc attgcagatg gttggtgatg atgactggcg
     2821 tattgccgtt ttgcaggatg gctgacaggg acttgaggtc tggattggcg atcaccacgc
     2881 aaccatcgct ggcgcgtggc ggtcggctat aggtgtcgct cggtgtgccg tgcagccaga
     2941 tgccgtggcc ttttcggcct tgcttgcgat cccattcgtt cgggtagttc aggggatagg
     3001 cggcatcgcc gtagaaatca ggcagcttgc gcgtcagctt gctaccggcg acatagacgc
     3061 cgaccggcgt acgcttgtcg ccttcggtct gtttttcgct gccgttcttg ccaacagtga
     3121 cgtagtaatc agcgacgtat tgcgggtggc cgttgtcgtt gcggtagaca tacaagcgcg
     3181 agttgctggt atcggcgacg atgg
//
LOCUS       DATMTW010000238         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_524709, whole genome shotgun sequence.
ACCESSION   DATMTW010000238 DATMTW010000000
VERSION     DATMTW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524709"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /gene="rpiA"
                     /locus_tag="VN084_04680"
     CDS             complement(<1..597)
                     /gene="rpiA"
                     /locus_tag="VN084_04680"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWT28784.1"
                     /translation="MNGKQLVATEAAKHVENGMLVGLGTGSTADLFIEAIASRMRQEK
                     LDLKVVSSSAASAAKAGELGLSLVDIAEITHLDLYVDGADEVTPDMTLLKGRGADLVK
                     EKLLAKACGQFIVLVDQSKLVSRIGEKYRIPVEVPAFAWQLVKRSLEQAGGKVTLRMK
                     EGQPAVSSHGSWILDVAFEPRLDAATLDDLLNSTPGVVE"
     gene            819..4598
                     /locus_tag="VN084_04685"
     CDS             819..4598
                     /locus_tag="VN084_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT28785.1"
                     /translation="MFCSGPDKVCQQADNARQLPAAEHLSVTVLRGLCILILCMGAFF
                     SASALAAERPINLHLKWYHQFQFAGYYAAVSQGYYQEEGLNVHLVEGGAGKHALKAML
                     DGQAEYAITDAEVVLDRLQGKPIVAIAAIFQHSPYIILSRADRGIRTVDDLLGKKVMF
                     EDGQGGVQFRAVLLREGLRVDDVKAIPHSWNLQDLIDGKVDAMSAYATAEPNQMRARG
                     VKPAILQGKDYGIDFYGDTLITTEHEVNDHPQRVEAMLRATRKGWEYALSHPDEIIDD
                     ILRMPGTRERGLSKEILHQEYEAMRPYILSDVVEIGHMNPGRWQHIADVFADLGEVPK
                     DYSLEGFLYLPEAARQAKLLHWAVGTMLSIAFVITVIVLWNLQMRRSVKRHTAELQDE
                     VAQRLRAEQALHASEQMVRLTFDTAAAGIAMSTLDGRTVLANPAYCEIVGYTEAELHD
                     IDQLDITHLDDVIENTQLLNDLLSGKIEAFVIQKRYIRKNRSTVWVKVSVSLLRHADG
                     TPANLIRVVENIEQQKQLETFKTIQNTILEHIAQGARLKSVLTSLVEMVEAQYPQCLC
                     SVLLLDDKQCLRTDAYSRLPDAYMQALDGVMIGSGVGSCGTAAFEKRRVVVSDIAKDP
                     LWKNYRGLAGKFGLKACWSVPILSSKKDVLGTFAVYSQTVHMPDTQELDLLSSSSHLA
                     GIAIERHLAEDHLRLLENSIATLNDIVLISEAEPFEEPGPRIVFVNKAFERLTGYTKE
                     EVIGKTPRILQGVNTQRSELDRIHAALKKWEPVRAELINYKKSGEEIWLDLNIVPLAD
                     ENGWYTHWVAIERDITERKLAEQEIQHLAFYDPLTKLPNRQLLLDRLEQQLVSTIRSD
                     HSGALLFIDLDNFKILNDSHGHDMGDFLLKQVAKRIGEAVRASDTVARLGGDEFVVIL
                     ENLSADETEAATQSQNVAEKILHSFSEPFRLPGLEHYTTPSIGIASLGNKAISVDEIL
                     KHADLAMYQAKAAGRNTFRFFNPEMQALISARIALEEDLRQAIRNDEFFLVYQPQVDQ
                     SGEVIGVEALLRWQHPGKGLIPPIHFIPLAEDTGLILPIGRKILDTACRQIERWAKLP
                     YAKHLEIAVNVSVRQFRQVDFVEQVFEVLDSTGADPTRLKLELTESLLVENVEDVIDK
                     MMRLKARGVGFSLDDFGTGYSSLAYLKRLPLEQLKIDQSFVRDILTDPNDAAIVRTTI
                     ALGNSLGLNVIAEGVETEAQRDFLHENGCVSYQGYLFGRPMPIDAIDRYLRDKAH"
     gene            complement(4611..>5176)
                     /locus_tag="VN084_04690"
     CDS             complement(4611..>5176)
                     /locus_tag="VN084_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HWT28786.1"
                     /translation="QQHPEIIIDLRLSDSITDAIEQQIDIGVRVGFLRDSSFIARSPA
                     KVSFFSVGAPSLIARVGKPTSIEDLLNLPTTGLVDSSTGRCWPWYFSDGQQFAPKATA
                     FITDDAETELDLVLNGIGFAQVPGCLAIPLIRSGKLVTVLDRFLAPPWDLYVYRPQRG
                     PVPTRTRLVYDRLLQTLADPARFPVTL"
BASE COUNT         1086 a         1554 c         1529 g         1007 t
ORIGIN      
        1 ctcgaccaca cccggtgtgc tgttcagcaa gtcgtcgagt gtggcggcgt caagcctggg
       61 ctcgaaggcc acatcgagga tccagctacc gtgcgaactg acggccggct gcccttcctt
      121 catgcgtaag gtgaccttgc cccctgcctg ttccaggctg cgcttcacca gttgccacgc
      181 gaaagcgggt acttcgaccg ggatacgata tttttcacca atccggctga ccagcttgct
      241 ttgatcgacc agcacgatga actgaccgca ggctttcgcc aggagcttct ctttcactag
      301 atcagcgcct cgacccttga gcaaagtcat gtcaggcgtc acctcgtcgg ccccgtcgac
      361 atacagatcc agatgcgtga tctcggcgat atccaccagc gacaggccga gctctccggc
      421 ctttgccgca cttgccgccg aactcgaaac caccttgagg tcgagcttct cctgccgcat
      481 gcgtgaggcg atcgcctcga tgaaaaggtc tgccgtcgag ccggtgccca gccctaccag
      541 catgccgttt tcgacatgct ttgcggcttc agtcgcgact agttgtttac cgttcatgat
      601 gtttcctcct tgcaaggtgt ggcgtgaacc acgaaaagcg ctcggaacgc cgggatgaca
      661 gggtatccgc ttgatttcgc atcgtcatct ttcatgccaa tcatggcaaa ataagactat
      721 attgtgcgta tgcatgcgat tgaaaattaa atcgctagaa catcggggag aggatgcccg
      781 tctgtcggtg ataaatgatc gaccacgagg cttagataat gttctgctca ggtccagaca
      841 aggtctgtca gcaagcggac aacgcccggc aactgccggc cgctgaacac ctctctgtta
      901 ccgtgcttcg tggcttgtgc atcctgatcc tgtgcatggg cgcgtttttc agcgcctcag
      961 cgttagccgc cgagcggccc atcaaccttc atctcaagtg gtatcaccag ttccagtttg
     1021 ccggctacta cgccgccgtg tcgcaaggct attatcagga agaagggctg aacgtgcatc
     1081 tcgtcgaggg cggcgctggt aagcacgcac tcaaggccat gctcgatggc caggcggaat
     1141 atgccatcac cgatgccgaa gtggtgctgg accgtttgca gggcaagcct atcgtcgcca
     1201 tcgccgccat cttccagcat tccccttaca tcatcctgag ccgggccgac agagggatcc
     1261 gtaccgtcga tgacctgctc ggcaagaaag tcatgttcga ggatggccag ggcggcgtac
     1321 agttccgcgc cgttctgcta cgtgagggtc tgcgtgtcga tgacgtcaag gcgattccgc
     1381 atagttggaa cctgcaggac ctgatcgacg gcaaggtcga tgccatgtcg gcctatgcga
     1441 cggcagagcc caaccagatg cgcgccagag gggtcaaacc ggcgatactg caaggcaagg
     1501 attacgggat cgatttctac ggcgatacgc tgatcaccac cgagcacgag gtcaacgatc
     1561 atccgcagcg ggtcgaggcg atgttgcgcg ctacgcgcaa gggttgggaa tacgcgctga
     1621 gtcatccgga cgagatcatc gatgacatcc tgcggatgcc gggtacgcgg gaacgcggcc
     1681 tcagcaagga gatcctgcac caggaatatg aagccatgcg gccttatatc ctgtccgatg
     1741 tggtcgagat cggtcacatg aatccggggc gctggcagca tatcgcagac gtctttgccg
     1801 atctgggcga agtgccgaag gactactctc tggaaggctt cctctatctg cctgaagcgg
     1861 cccgtcaggc caagctgctg cattgggcgg tcggcaccat gctcagcatc gcattcgtca
     1921 tcaccgtgat cgtgctctgg aacctgcaga tgcgtcgcag cgtcaagcgg cataccgccg
     1981 aacttcagga tgaagtcgcc cagcgcctgc gtgccgaaca agcgcttcat gccagcgagc
     2041 agatggtgcg cctgaccttc gataccgcgg ctgccggcat cgccatgagt acgctggatg
     2101 gtcgcacggt gcttgccaat ccggcctatt gcgagatcgt cggttacacg gaagcggaac
     2161 tgcatgacat cgatcaactg gacatcacgc acctggacga cgtgatcgag aacacacaac
     2221 tgctcaacga cctgctgtcg ggcaagatcg aagcgttcgt catccagaaa cgctatatcc
     2281 gcaagaaccg cagcacggtc tgggtcaagg tcagcgtctc tttattgcgc catgccgacg
     2341 gcacgcccgc caacctgatc cgcgtggtcg agaacatcga gcagcagaag cagctggaaa
     2401 cattcaagac catccagaac acgatccttg agcacatcgc ccaaggcgca aggttgaagt
     2461 cggtgctcac atcactggtg gaaatggtgg aagcgcagta tccccagtgc ctctgctccg
     2521 tactgctgct cgacgacaag caatgcctgc gcaccgacgc ctattcgcgc ctgccggacg
     2581 catacatgca ggcgctcgac ggcgtcatga tcggctctgg cgtcggctcg tgcggcacgg
     2641 ctgccttcga gaagcgccgc gtggtggtca gcgatatcgc caaggatccc ttatggaaga
     2701 attaccgcgg actggcgggc aagttcggcc tcaaggcctg ctggtcggta cctatcctgt
     2761 cgtcgaaaaa ggacgtgctc gggacgtttg ccgtttattc acagacagtg cacatgccgg
     2821 atacgcaaga gctggatctg ctgagttcat catcccatct ggccgggatc gccatcgaac
     2881 gccacctggc cgaagaccac ctgcgcctgc tggagaacag catcgctacg ctcaatgaca
     2941 tcgtgctgat ctccgaggcg gagccgttcg aggagcccgg tccacgcatc gtcttcgtca
     3001 acaaggcctt cgagcggctc accggctata ccaaggaaga agtcatcggc aagacgcccc
     3061 gcatcctgca aggcgtgaac acccagcgca gcgagctcga tcgtatccac gccgcgctga
     3121 agaaatggga gccggtaagg gcggagctca tcaattacaa aaagagcggc gaagagatct
     3181 ggctggacct caatatcgtc cctttggcag acgagaacgg ctggtacacc cattgggtcg
     3241 ccatcgaacg cgatatcacc gaacgcaagc tggctgagca ggagatccag catctggcct
     3301 tttatgatcc gctgaccaag ttgcccaatc gtcagctgct gctcgaccgc cttgaacagc
     3361 aactggtctc caccattcgc agtgaccata gcggcgcgct gctgttcatc gatctggata
     3421 atttcaagat cctcaacgat tcccacggcc atgacatggg agatttcctg cttaagcagg
     3481 tggcaaagcg catcggcgag gcggtcaggg cttcggatac cgtggcccga ttgggaggcg
     3541 atgagtttgt ggtgatcctg gagaatctga gtgctgacga gacggaagct gccacccaat
     3601 cgcagaacgt cgcggaaaag atcctgcaca gcttcagcga gccattccgc ttgccgggcc
     3661 tcgaacatta caccacgcca agtatcggca tcgcctcgtt aggcaacaag gcgatcagcg
     3721 tcgacgagat cctcaagcat gccgatcttg ccatgtacca ggccaaggcg gccggccgta
     3781 acaccttccg cttcttcaat cctgagatgc aggccctgat ctccgcacgc atcgcgctgg
     3841 aggaagatct acgccaggcg atccgcaatg acgaattctt cctcgtttac cagccgcagg
     3901 tcgaccagtc cggagaggtc atcggtgtcg aagcgctatt gcgctggcag cacccgggca
     3961 agggactgat cccgccgatc catttcattc cgctggcaga agacaccggg ctgatcctgc
     4021 cgatcgggcg caagatcctt gataccgcct gccgccagat cgagcgctgg gccaagctgc
     4081 cctatgcgaa gcaccttgag atcgccgtca acgtcagtgt ccgccagttc cgccaggtcg
     4141 atttcgtgga gcaggtgttc gaagtgctcg atagtaccgg cgctgacccg acccggctca
     4201 aactggaatt gaccgagagc ctgctggtgg agaacgtcga ggatgtcatc gacaagatga
     4261 tgcggctgaa ggcacgcggg gtcggattct cgctcgatga tttcggcacg ggatattcct
     4321 cgctggctta tctcaagcgc ctgccgctgg agcagctcaa gatcgatcaa tctttcgtgc
     4381 gcgatatcct caccgatccc aacgatgccg ccatcgtccg cacgaccatt gcgctcggca
     4441 acagcctggg cctcaacgtc atcgccgagg gcgtggagac cgaagcccag cgcgatttcc
     4501 tgcatgagaa cggttgcgtc tcatatcagg gttatctctt cggcaggccg atgccgatcg
     4561 acgcgatcga ccgctacctc agggacaagg cccactgaca gccacgagac tcaaagtgtg
     4621 acggggaatc gggccgggtc ggccagcgtt tgcagcaggc ggtcgtagac cagacgtgtc
     4681 cgtgtcggca ccggcccacg ttgcggccgg tagacataga gatcccaagg cggcgcgagg
     4741 aatcgatcca gtaccgtgac cagcttgccg ctgcggatca aggggatcgc caggcatccg
     4801 ggcacctggg cgaagccgat gccgttgagc accaggtcga gctcggtctc ggcgtcgtcc
     4861 gtgatgaaag ccgtggcctt gggcgcgaac tgctggccat cggagaaata ccagggccag
     4921 caacggccgg tgctgctgtc gacgagtccg gtggtcggca ggttgagcag atcctcgatg
     4981 ctggtgggct tgccgacgcg ggcgatcagg ctcggggcgc cgactgaaaa gaacgatacc
     5041 ttggccggcg agcgggcaat gaagctgctg tcgcgcagaa agccgacgcg cacgccgata
     5101 tcgatctgct gttcgatggc gtcggtgatg gaatcggata gcctcaggtc gatgatgatc
     5161 tccgggtgct gttgcg
//
LOCUS       DATMTW010000239         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_357687, whole genome shotgun sequence.
ACCESSION   DATMTW010000239 DATMTW010000000
VERSION     DATMTW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357687"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..2110
                     /locus_tag="VN084_04695"
     CDS             92..2110
                     /locus_tag="VN084_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWT28787.1"
                     /translation="MLSKLNSPQRQAVKYLDGPLLVLAGAGSGKTRVITHKIAYLINE
                     CGYLPREIAAITFTNKAALEMQERVGHLLEGKQSKGLTIATFHSMGLQMLRAEAKLLG
                     YKPQFSILDSSDSFKILSDVLATTDKQLLRKTQWQISQWKNGFINPDQAKAQADEELT
                     VAAAKVYQIYQQTLKAYQAVDFDDLIKLPVELFEQHPEALEKWQRKLKYLLIDEYQDT
                     NACQYKLVKLLTGVEGRFTAVGDDDQAIYGWRGADVENLRQLTEDWSRLKVIKLEQNY
                     RSTVRILRAANQVIANNPKLFDKKLWSDLGTGEHIQVSAARDEEHEAEAVVMKLTAHK
                     FEHRTKYSDYAILYRGNHQARILEQQLRNHKVPYTISGGQSFFDKAEIKDVVAYLRLI
                     ANEDDDPAFIRAATTPKKGIGNTTLERLGEYASREHMSLFAAAFEGEFQRELGQRQLD
                     DLLTFCQFISRIQERAAKDPAGEVLESLMNAIQYEPFLYDSEEPRAAESKWKNVQEFI
                     GWLTRKGEGSGDDDERNLLQLTQMVSLMSMLEGRENGEPDAVKLSTLHAAKGLEFGHV
                     FLIGCEEGILPHRESVDNGKIEEERRLMYVGITRAQKSLNISWCKKRRRAGENEVCEP
                     SRFIGELPQDDVRHFGGPQADPEVSKEMARERMAHIRAMLGNKSPAAS"
BASE COUNT          532 a          735 c          682 g          370 t
ORIGIN      
        1 gcgcatttta tatgcaccga gccggatact aaatacgagc gctcgaatag aagtgacacc
       61 tcaccccatc agcggctaaa cttgcgcccg catgttatcc aaactcaact ccccccaacg
      121 tcaagcagtc aaatacctgg atggccccct gctggtgctg gccggtgccg gcagcggcaa
      181 gacgcgcgtc atcacgcaca agatcgccta tctgatcaac gaatgcggct acctgccgcg
      241 cgagatcgcc gccatcacct tcaccaacaa ggccgcgctg gagatgcagg agcgggtcgg
      301 ccacctgctg gagggcaagc agagcaaggg cctcaccatc gccaccttcc attcaatggg
      361 gctgcagatg ctgcgcgccg aagccaaatt gctgggttac aagccgcagt tctccatcct
      421 cgattcgtcc gatagcttca agatcctgtc cgatgtgctg gcgacgacgg acaagcagtt
      481 gctgcgcaag acccagtggc agatctcgca atggaagaac ggtttcatca atccggacca
      541 ggccaaggcg caggcggatg aagaattgac ggtggccgcc gccaaggtct accagatcta
      601 ccagcagacg ctgaaggcct atcaggcggt ggatttcgac gacctcatca agctgcctgt
      661 cgaattgttc gagcagcacc cggaagcgct ggaaaaatgg cagcgcaagc tcaaatacct
      721 gctgatcgac gaataccagg acaccaacgc ctgccagtac aagctggtga agctgctgac
      781 cggggtcgaa ggccgcttca ccgcggtggg cgacgacgac caggccatct atggctggcg
      841 cggcgccgat gtcgagaacc tgcgccagct gaccgaggac tggtcgcgcc tcaaggtgat
      901 caagctggag cagaactacc gctccaccgt gcgcatcctg cgcgccgcca accaggtcat
      961 cgccaacaac cccaaactgt tcgacaagaa gctgtggagt gacctcggca ccggcgagca
     1021 tatccaggtg agcgccgccc gcgacgagga gcacgaagcc gaagccgtgg tcatgaagct
     1081 caccgcacac aagttcgagc accgcaccaa gtattcggat tacgccatcc tctaccgcgg
     1141 caaccaccag gcgcgcatcc tcgaacagca actgcgcaac cacaaggtgc cctacaccat
     1201 ctccggcggg caatcgttct tcgacaaggc cgagatcaag gatgtagtcg cctacctgcg
     1261 cctgatcgcc aacgaggatg acgatccggc gttcatccgc gccgccacga cgccgaagaa
     1321 aggcatcggt aacaccacac tggagcggct cggcgagtac gcaagccgcg aacacatgtc
     1381 gctgttcgcc gcggcgttcg aaggcgaatt ccagcgcgag ctcggccagc gccagctcga
     1441 cgacctgctg actttctgcc agttcatcag ccgcatccag gagcgcgcgg cgaaagaccc
     1501 ggctggcgaa gtgctggaaa gcctgatgaa cgccatccag tacgagccct tcctctacga
     1561 ttccgaagag ccccgcgctg ccgagagcaa atggaagaac gtgcaggaat tcatcggctg
     1621 gctcacgcgc aagggcgaag gcagcggcga tgacgacgaa cgtaacctcc tgcaattgac
     1681 gcagatggtg tcgctgatga gcatgctcga aggccgcgag aacggcgagc cggacgcggt
     1741 caagctctcc acgctgcatg ccgccaaggg cctggagttc ggccacgtgt tcctcatcgg
     1801 ctgcgaggaa ggcatcctgc cgcaccgcga aagcgtcgat aacggcaaga tcgaggaaga
     1861 acgtcgactc atgtatgtcg gcatcacgcg tgcgcagaaa tcgctcaata tctcctggtg
     1921 caagaaacgc agacgcgccg gcgagaacga ggtctgcgaa ccaagccgct tcatcggcga
     1981 actgccgcag gatgatgtgc gccattttgg cggcccacag gcagaccccg aagtgagcaa
     2041 ggagatggcg cgcgagcgca tggcgcacat ccgcgccatg ctgggcaaca agtcgccagc
     2101 cgcgagctga gttggcgcgg caaggccgga gatgccggct agcggctttg ctgggattcc
     2161 ctaggcgcct caaggctgcc tggcggaacc tttgacgttg cgcggactca gtttttgtac
     2221 ggcccctgct ctttgaaccg gcttagccgg ttcaacctga cgaaccatga atgaatgacg
     2281 ccaccaggcg atgcagcctg gcatgtcttt gagcaccta
//
LOCUS       DATMTW010000240         5082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_558463, whole genome shotgun sequence.
ACCESSION   DATMTW010000240 DATMTW010000000
VERSION     DATMTW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558463"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..922
                     /gene="trxB"
                     /locus_tag="VN084_04700"
     CDS             2..922
                     /gene="trxB"
                     /locus_tag="VN084_04700"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWT28788.1"
                     /translation="MILGSGPAGYSAAVYAARANLKPVLVTGIAQGGQLMTTTEVDNW
                     PADADGVQGPELMARFQKHAERFNTEMIFDHIHTAKLKEKPIRLIGDAGEYACDALII
                     ATGASAQYLGLPSEEHFSGKGVSACATCDGFFYRNQEVAVIGGGNTAVEEALYLANIA
                     SKVTLVHRRDKFKAEAILVDKLMQRVQEGKIELQLHHVLEEVLGDNSGVTGMRIKHNQ
                     TNETKDIALKGVFIAIGHKPNTDIFADELEMENGYIVTKGGREGNATATSIPGVFAAG
                     DVQDHIYRQAVTSAGSGCMAALDAERYLDV"
     gene            1010..1555
                     /locus_tag="VN084_04705"
     CDS             1010..1555
                     /locus_tag="VN084_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWT28789.1"
                     /translation="MTVSNPDQDDDDIELFRAAVQGARPVKSTRVPITPPRPKPVPRQ
                     FIRDEQQALRDSLSDDYIPAHELESGEELLYLREGHSPDILSKLRRGHWVVQAAIDLH
                     GLIADEARLYVATFLADCKKRGIRCVRIVHGKGLGSRNREPVLKHKLRNWLMQKDEVI
                     AYAQARPQDGGSGAVIVLLKA"
     gene            1681..2856
                     /locus_tag="VN084_04710"
     CDS             1681..2856
                     /locus_tag="VN084_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWT28790.1"
                     /translation="MLNSWLDSRRALWRAYPLSLALLLGLSLATPASASEKAVAKPPP
                     SPGKITKKVDEVILPPGSPQLASLRIEPVVEIDAPAAEPLNGKIAFDENHTARISSPV
                     AGRVSRIFVEVGDTVKAGQKLLAMDSPDLGEAIADVHKAQSELLLKRQAFERSKLLLD
                     EGVIARKDHENNQAALAEAEAEARRTRAKLENLGGARDAGSETYSVRAPIAGVVVDRQ
                     VNPGNEVRPDAAAPLFIITDPAHLWATIDLPERDLGKVSSGQAVNIEVEAYPGEIFSG
                     HIQSIGAMVDPTTRRIPVRCVIDAQNKLKPEMYARITPLSPSREKVIRTPNEALITEG
                     IHNYVFVEISPGHLKKKLVVLNSQGREYATIKQGLSKGERLVTGGAVLLNSELSAGK"
     gene            2864..>5082
                     /locus_tag="VN084_04715"
     CDS             2864..>5082
                     /locus_tag="VN084_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWT28791.1"
                     /translation="MLEKLITFSLQQRGFVLLGTVAIVISGWFALQNLPIEAFPDVED
                     VHVGIVTQVPGLAPEEVERVVTLPIEHEMSGVPHLSQMRSVSITGLSVVTLTFSEHTD
                     DYFARQQVIEHLQGADLPAAYQPTLAPLSTAVGEIFRYLVEAPKDMPQRDIRAIQDWQ
                     IRPILRTVPGVADITSFGGAVKEYQVRIDPYLLKKFDLTIDQISQALSTNSSNVGGGL
                     MHRGDESLVIRGIGLYKSLDDIARTVITSRQGKPILVGDIGQVVIGDRTRSGIVAFND
                     RDDVVEGIVIMNKGQNPAKVIADLREKVKEINAKLPPGVRVAPFYDRTTLVDHTVHTV
                     TENLIVGALLVVALLVVFLRNWRAALIVASIIPLSLLFAFIIMDVRGVSANLISLGAV
                     DFGIIIDSAVVLVEALMVRLAFAQVDQFPQHANYGWRLHTLKQTAIEMGRPILFSKII
                     IILAFLPIFTFQRVEGKIFSPMAFTLSFALLGAILLTLTLVPALLSYTMRREDMAEKH
                     SPWMEAIQKGYRDFLLHMAKMRKLTVGVSVALLALSLLLAPRLGSEFLPKLDEGNIWL
                     TISLPPSTGLEKTKDVERHVRKILESYQEINLVVSHVGRPDDGTDPKGPNNIEILADL
                     KPHDAWRFPTKEALVNDISQKIHQIPGIPTNFSQVIQDSVEEALSGVKGEIAVKIFGS
                     DLNILEAKADEVVDVLNGIQGATDVAAIRISGNAELDIRLDRAKLARYGMSVNDVNTT
                     "
BASE COUNT         1075 a         1630 c         1464 g          913 t
ORIGIN      
        1 gctgattctg ggttccggcc cagcgggcta ctctgccgct gtctatgctg cgcgcgcaaa
       61 cctcaagccg gtgctggtca caggcatcgc ccagggcggt caactgatga ccacgaccga
      121 ggtcgacaac tggcccgcgg atgccgatgg cgtgcaaggc ccggaactca tggcgcgttt
      181 ccagaagcat gccgagcgct tcaacacgga aatgatcttc gaccatatcc atacagccaa
      241 actcaaggaa aagcccatcc gcctgatcgg cgatgccggc gaatacgcct gcgacgcgtt
      301 gatcattgcc accggcgctt ccgcccagta tctgggcctg ccttccgaag aacacttctc
      361 gggcaagggc gtttcggcct gcgcgacatg tgacggcttc ttctatcgca accaggaggt
      421 ggccgtgatc ggcggtggca acacagcggt cgaggaagcg ctgtacttgg cgaacatcgc
      481 cagcaaggtg accctggtgc atcgccgcga caagttcaaa gccgaagcca tcctggtcga
      541 caaattgatg cagcgcgtac aggaaggcaa gatcgaactg caactgcacc atgtgctgga
      601 agaagtgctg ggcgacaaca gcggcgtgac tggcatgcgc atcaagcaca accagacgaa
      661 cgagaccaag gacatcgcgc tcaagggcgt gttcatcgcc atcggccaca aacccaacac
      721 cgatatcttc gcggacgagc tggagatgga gaacggctac atcgtcacca agggtggccg
      781 tgaaggcaac gccaccgcga ccagcatccc cggggtcttc gcggcgggcg acgtacagga
      841 ccatatctac cgccaggccg tgaccagcgc aggttcgggc tgcatggcgg cgcttgacgc
      901 cgagcgctat ctcgacgttt aggcgcaaac cgccggtaac ggaaagctaa cccaagaacg
      961 cactatcagg gcagaatacc agcgctgata tgtgcgtttt tttaatggcc tgactgtgtc
     1021 gaaccctgac caagatgatg acgatatcga gcttttccgc gcggccgtcc aaggcgcacg
     1081 cccggtaaag tcgacgcgcg tccccatcac cccaccccgt cccaaaccag ttcccaggca
     1141 gttcatccgc gatgagcagc aggccttgcg cgattcgttg agcgacgatt acatcccggc
     1201 gcacgaactc gaaagcggcg aagaactgct ctacctgcgc gaaggccact cgccggacat
     1261 cctgagcaaa ctgcggcgcg gacactgggt ggtgcaagcg gcgatcgacc tgcatggcct
     1321 gatcgccgac gaggccaggc tctatgtcgc gaccttcctg gccgactgca agaaacgcgg
     1381 catccggtgc gtgcgcatcg tgcatggcaa gggtctggga tcgcgaaacc gtgaacccgt
     1441 actcaagcac aagctgcgca actggctgat gcaaaaggac gaggtgatcg cctatgccca
     1501 ggcccggcca caggatggtg ggagtggcgc cgtcatcgtc ctgctcaagg catgaaatta
     1561 tttttgctgg ataggaattt ttcgcatagc gtcacagtca atgcatcaac acttgctccg
     1621 gcatccgtat cgaccgctcc gatcacccct gactcccact gacaccgaat gaagcccctt
     1681 atgcttaact cctggcttga ctcccggcgc gccctttggc gtgcctaccc gctatcactg
     1741 gcgttattgc tgggcctgag cctggccaca ccggcttcgg caagcgaaaa agccgttgcc
     1801 aagccgccac ccagccctgg caagatcacc aagaaggtcg atgaagtcat cctgccgccg
     1861 ggctcaccgc agctggccag tctgcggatc gagccagtgg tcgagatcga tgcgcccgcg
     1921 gcagaaccgc tcaacggcaa gatcgccttc gacgagaatc acaccgcccg catcagttcg
     1981 ccggttgcag gccgcgtctc gcgtatcttc gtggaggtcg gcgataccgt caaagccggc
     2041 cagaagctac ttgccatgga ttcgccagac ctcggcgagg ccatcgcaga cgtgcacaag
     2101 gcgcagagcg aactgctact gaaacgccag gccttcgaac gaagcaagct gttgctcgac
     2161 gaaggcgtga tcgcacgcaa ggatcacgag aataatcagg ccgcgctggc ggaagccgag
     2221 gccgaggccc gtcgcactcg ggcaaaactt gagaatcttg gcggcgctcg cgatgcaggc
     2281 agcgaaacct acagcgtgcg tgcgccgatc gccggcgtgg tcgtcgaccg ccaagtcaac
     2341 ccgggcaacg aagttcggcc tgacgccgcc gctccgctat tcatcatcac cgacccggca
     2401 cacctgtggg cgacgatcga cctgcccgag cgcgacctgg gcaaggtcag cagcggccag
     2461 gccgtcaata tcgaggtcga ggcctatccg ggcgagatct tcagcggcca tatccaatcg
     2521 atcggtgcca tggtcgaccc gaccacacgc cgcattcccg tgcgctgtgt gatcgatgcc
     2581 cagaacaagc tgaagccgga gatgtatgca cgcatcacgc cgctcagtcc tagcagggaa
     2641 aaggtcatcc gcacacccaa cgaggccctc atcacggaag gtatccacaa ctacgtcttt
     2701 gtcgaaatca gccctggcca cctgaagaag aaactggtcg tgctgaattc acaaggtcgc
     2761 gagtatgcga ctatcaagca gggcctgagc aagggtgagc gcctagtcac cggtggcgca
     2821 gtcctgctca actccgaact ctcagccggc aagtagccct cccatgcttg aaaaactgat
     2881 caccttttcg ctgcagcagc gcggcttcgt gctgctgggc acggtagcca tcgtcatctc
     2941 cggctggttc gcgctgcaga acctgccgat cgaggcattc ccggatgtcg aggatgtgca
     3001 tgtcggcatc gtcacccagg tgccgggtct cgcgccagag gaagtcgagc gtgtcgtcac
     3061 gctgcccatc gaacacgaga tgagtggcgt gccccacctg agccagatgc gttctgtctc
     3121 catcactggc ctatcggtag tgaccctgac cttctccgag cataccgacg attacttcgc
     3181 tcgccagcag gtcatcgaac acctgcaggg cgcagacctg cccgccgcct accagccgac
     3241 gctggcacca ctatccacgg ctgtcggcga gatcttccgt tacctggtgg aagcccccaa
     3301 ggatatgccg caacgcgaca tccgcgcgat ccaggattgg cagatccggc ccatcctgcg
     3361 caccgtaccc ggtgtcgccg atatcaccag tttcggcggt gcggtcaagg aataccaggt
     3421 gcgcatcgac ccttacctgc tgaagaaatt cgatctcacc atcgaccaga tcagccaggc
     3481 gttgtccacc aacagcagca acgtcggcgg cggcctcatg caccgtggcg acgaatcgct
     3541 ggtcatccgc ggcatcggcc tctataaaag tctggacgac atcgcccgca ccgtcatcac
     3601 gtcgcgccag ggcaaaccga tcctggtcgg cgatatcggc caggtcgtca tcggcgatcg
     3661 cacgcgctcg ggtatcgtcg ccttcaatga ccgcgacgat gtggtcgaag gcatcgtgat
     3721 catgaacaag ggccagaacc cggccaaggt catcgccgac cttcgggaaa aggtcaagga
     3781 gatcaacgcc aagctgccgc ctggtgtccg cgtcgcgccg ttctacgatc gcaccacact
     3841 ggtcgaccat accgtgcata ccgttaccga gaacctcatc gtcggcgccc tgctggtggt
     3901 cgccttgctg gtcgtgttcc tgcgtaactg gcgcgcggcg ctgatcgtcg cttccatcat
     3961 cccgctatcg ctgttgttcg ctttcatcat catggatgtg cgcggggtat cggccaacct
     4021 gatctcgctt ggggcggtgg acttcggcat catcatcgat agtgccgtcg tgctggtcga
     4081 ggccctgatg gtccggctgg cttttgccca ggtcgatcag ttcccgcagc acgcgaacta
     4141 cggctggcgc ctgcataccc tgaaacagac cgcgatcgag atgggacgac cgatcctgtt
     4201 ctcgaagatc atcatcatcc tggctttcct cccgatcttc acgttccagc gggtcgaggg
     4261 caagatcttt tcgcccatgg ctttcacgct aagcttcgcg ctgctcggag ccatcctgct
     4321 gacgctgaca ctggtaccgg cgctactctc atacaccatg cgtcgtgaag acatggccga
     4381 gaagcacagc ccgtggatgg aggcgatcca gaagggttac cgcgattttc tgctgcatat
     4441 ggcgaagatg cgaaaactca ccgtcggcgt atcggtggcc ttgctcgcgc tttcgctgct
     4501 actggcgccc cgcctcggct cggagttcct gcccaagctg gatgaaggca acatctggct
     4561 gaccatcagc ctgccgccgt ccaccgggct cgagaagacc aaggatgtcg aacgccatgt
     4621 acggaaaatc cttgagagct atcaggagat caatctggtg gtgagccatg tcggacgccc
     4681 tgatgacggc accgacccca aggggccgaa taacatcgag atcctcgctg acctcaaacc
     4741 gcacgacgca tggcgattcc cgaccaagga agcgctggtc aacgacatct cgcaaaagat
     4801 ccatcagatc ccgggtatcc cgaccaactt ctcgcaagtg atccaggata gcgtggaaga
     4861 agcgctttcc ggcgtcaagg gtgagattgc ggtcaagatc ttcggtagcg acctcaatat
     4921 cctggaagcc aaggccgacg aagtggtcga cgtgctgaac ggcatccagg gcgcgaccga
     4981 tgtcgctgcc atccgcatct cgggcaacgc cgaactcgat atccgtctgg atcgcgccaa
     5041 gctggcgcgc tacggcatga gcgtcaacga cgtcaacacc ac
//
LOCUS       DATMTW010000241         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_659579, whole genome shotgun sequence.
ACCESSION   DATMTW010000241 DATMTW010000000
VERSION     DATMTW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659579"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VN084_04720"
     CDS             <1..1878
                     /locus_tag="VN084_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWT28792.1"
                     /translation="LTKDQILELYINQIYLGQRSYGFAAASQVYYGKPLEKLSVAETA
                     MLAGLPKAPSRYNPFANPKRATARQHYVLGRMKALRFIDEPAYEEALKEPTRLRTSRQ
                     VRDVQADYVAEIARQVMYARYKDDIYNSGMRVYTTIRKQNQEAANAAVLQGIVDYERR
                     HGYRGPEGLVRATDKAEADTHNWMDSALDDIDEFNGLIPAIVTSATPKLLKAHTKHGE
                     DIEISGDGLALVQKTLNEKDAAKRKLKPGAVIRVVQLADRWQVTQLPQVESAFVGLDP
                     ENGAVRALVGGFDFHRNKFNHVTQAWRQPGSSFKPFIYSASLEKGFTPASIIEDEPVF
                     LSAEETGSGEGWEPRNFDNKYDGPIRMRTALTKSKNMVSIRILQSIGVDYARDYITRF
                     GFSPKDHPPYLAMALGAGSVTPWQMAGAYAVFANGGYRVRPYLISKIVDSNGKVIEQT
                     KFDKAGDPSKRVIDSRNAYLMTSMMQDVARIGTASKARQIGRYDLAGKTGTTNNQIDA
                     WFAGFNPKQVAIAWMGYDKPRTLGSNETGGHAALPIWIDYMSNVLKGVPDNPYPVPDG
                     VSSIKIDPLTGTKVGDGESGIYEYFYQEYPPPEIEQHFTPIPGFTTSNLGSDPRADQL
                     Y"
     gene            1896..2549
                     /locus_tag="VN084_04725"
     CDS             1896..2549
                     /locus_tag="VN084_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28793.1"
                     /translation="MAKDIQSRQSQLRQLIAQQAARMMAEEGISDYAYAKRKASRQLG
                     ATDINCMPTNAEIEDEIRLFHEIYNSEDQPAHLRQLRADALLVMQLLERFEPHLTGAV
                     WDGTAGKYAETHIHLFADSLKDVEMFLLNQQIPYETNEKSYRVSNAAADKKGGERRKV
                     PMFTLEGPHGAIKLSVFEIDDMRIPTKHPGNGSNASRADISDLKALIASTAALTESE"
     gene            complement(2578..3570)
                     /gene="hemB"
                     /locus_tag="VN084_04730"
     CDS             complement(2578..3570)
                     /gene="hemB"
                     /locus_tag="VN084_04730"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWT28794.1"
                     /translation="MSKFPLSRPRRMRKDDFSRRLMREHVLTVDDLIYPVFVLDGERR
                     TENVASMPGVQRQSIDVLLETAAECVALGIPALALFPVVDQSLKSLDANEAFNPQGLV
                     PRTVRALKEAFPDLGVITDVALDPYTTHGQDGIIDASGYVLNDETTAVLVKQALCHAA
                     AGADVVAPSDMMDGRVGAIRQALEQAGHIHTRILAYSAKYASAFYGPFRDAVGSSANL
                     GKGNKYNYQMDPANSDEAIREVALDLDEGADMVMVKPGMPYLDIVRRVKAQFGVPTYA
                     YHVSGEYAMLKAAAQNGWLDERACVLEAMLAFKRAGADGVLTYYALDVARWLKA"
     gene            complement(3668..4456)
                     /gene="trpC"
                     /locus_tag="VN084_04735"
     CDS             complement(3668..4456)
                     /gene="trpC"
                     /locus_tag="VN084_04735"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWT28795.1"
                     /translation="MSDILNKILNVKQAEVEAAMAEKPLAILQREAMAMPPARDFVGA
                     IRGKIAAGKPAVIAEIKKASPSKGVIRADFRPAEIAASYADNGAACLSVLTDQQFFQG
                     SAEYLKQARAACAIPVLRKDFMIETYQVYEARAMGADCILLIAAALNLPRMRQLEAIA
                     HDLGMAVLVEVHNGEELDLALQLDTPLLGINNRNLRTFEVTLQTTFDLLARIPADKIV
                     VTESGIFTPDDVALMRSHDVHTFLVGEAFMRQPDPGLELARVFS"
     gene            complement(4494..4934)
                     /locus_tag="VN084_04740"
     CDS             complement(4494..4934)
                     /locus_tag="VN084_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HWT28796.1"
                     /translation="MLIVAIAIVYTVLQYPSSGLAQLPQQVAVATEQTAGDIEAIRHA
                     FDGRLSDIQVTGKGKVLKVLPDDGNGARHQRFILQLPVGITVLVAHNIDLAPRVPRLA
                     VGDVVEFNGEYVWNDKGGVVHWTHHDPHGQHAPGWLRHDGKTYE"
     gene            complement(4943..5968)
                     /gene="trpD"
                     /locus_tag="VN084_04745"
     CDS             complement(4943..5968)
                     /gene="trpD"
                     /locus_tag="VN084_04745"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWT28797.1"
                     /translation="MAVVFKEVLQQLLNRQDLTHEQMLAIMRQVMQGELTPAQIAGLL
                     IGLRMKGETVDEISAAAQVMRELSTKVEIHDTGHLVDTCGTGGDGIQTFNVSTVSAFV
                     AAAAGARVAKHGGRSVSSTCGSADVLEALGVNVNLTPQQVATAVDKIGIGFMFAPNHH
                     SAMKHAAPVRRELGVRTLFNLLGPMTNPAGAKRQVMGVFHKDLTGLLARVLQRLGSEH
                     VMVVHGADGMDEISFTGDTYVAELKHGEVHEYVIHPSQFGLPVHALTTIRIQDAQASR
                     AMVLDVLAGKTGPARDIVLLNAGAAIYVAGVAESMAAGIALAAEVIDSGAAAHKLEQL
                     KAIAVSA"
     gene            complement(5968..>6399)
                     /locus_tag="VN084_04750"
     CDS             complement(5968..>6399)
                     /locus_tag="VN084_04750"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWT28798.1"
                     /translation="PGPCTPNEAGISVPLIKAFAGKIPMLGVCLGHQSIGQAFGGNIV
                     HAKQLMHGKTSQIHHKDTGVFKGLPNPYTATRYHSLVIERETIPDCLEITAWTEDENG
                     AFGEIMGVRHKTLPIEGVQFHPESILTEYGHELLDNFLKAY"
BASE COUNT         1326 a         1998 c         1854 g         1221 t
ORIGIN      
        1 ctgaccaagg accagatcct cgagctctat atcaaccaga tctatcttgg gcaacgttcc
       61 tacggcttcg cagcggcatc acaggtctac tacggcaagc ccctggaaaa gctcagcgtc
      121 gccgagaccg ccatgctggc cggcctgcca aaggctccat cccgctacaa cccgttcgcc
      181 aatccgaaac gcgctacagc ccgccagcat tatgtgctgg ggcggatgaa ggcgttgcga
      241 ttcatcgacg agccggctta tgaggaggca ctcaaggagc cgacacggtt acgtacctcg
      301 cggcaggtcc gcgatgtgca ggcggattat gtggccgaga tcgctcgaca ggtgatgtac
      361 gcgcgttaca aggatgacat ttataacagc ggcatgcggg tctataccac gatccgtaaa
      421 cagaaccagg aagccgccaa cgcggcggtc ttgcaaggca tcgtcgatta tgaacggcgc
      481 catggctatc gggggccgga aggtctcgtg cgcgcgaccg acaaggcaga agccgacacc
      541 cataactgga tggatagcgc gctcgatgat atcgacgagt tcaatggcct gatcccagcg
      601 atcgtcactt cggccactcc gaagctgctc aaggcccata caaagcatgg cgaggatatc
      661 gagatctcgg gcgatggctt ggcgctcgta caaaagacgc tcaacgagaa ggatgcggcg
      721 aagcgcaagc tcaagccggg cgccgtgatc cgcgtagtac agctggctga tcgctggcag
      781 gtcacgcagc taccgcaagt cgaatcggcc ttcgtcggcc tcgatcccga gaatggtgct
      841 gtgagggcgc tggtgggcgg cttcgatttc catcgcaaca aattcaacca tgtcacccag
      901 gcatggcgtc aacccggctc cagcttcaaa ccattcatct actccgcttc actggaaaag
      961 ggcttcacgc ctgccagcat catcgaagac gagcctgtgt tcctcagcgc cgaagaaact
     1021 ggcagtgggg agggatggga accacgcaat ttcgataata aatacgacgg accgatccgc
     1081 atgcgcactg cgctgaccaa atccaagaac atggtctcca tccgcatcct gcagtctatc
     1141 ggcgtcgatt acgcacgcga ttacatcacg cgcttcggct tctcgccgaa ggaccatccg
     1201 ccatacctgg cgatggcatt gggcgcaggt tcggtcacgc cgtggcagat ggcgggtgcc
     1261 tacgccgtct tcgccaatgg cggctaccgg gtacggccgt atctgatcag caagatcgtc
     1321 gacagtaacg gcaaggtcat cgagcagacc aagttcgaca aggccggcga tccatcgaag
     1381 cgtgtcatcg acagccgtaa cgcctatctc atgacgtcga tgatgcagga cgtcgcccgc
     1441 atcggcaccg cctccaaggc gcgccagatc ggacgctacg acctcgccgg caagaccggc
     1501 accaccaaca accagatcga tgcctggttc gccggcttca atccaaagca ggtcgcaata
     1561 gcctggatgg gctacgacaa accgcgcacg ctgggcagca acgagaccgg cgggcatgcc
     1621 gccctgccga tctggatcga ctacatgtcc aatgtgctga agggcgtgcc ggataaccct
     1681 tatccggttc ccgacggcgt cagctcgatc aagatcgacc cactgaccgg gaccaaggtc
     1741 ggtgatggcg agagcggtat ctacgagtat ttctatcagg aatacccgcc gccagagatc
     1801 gagcagcatt tcacaccgat cccaggcttc acgacttcga acctggggag cgaccccaga
     1861 gccgaccagc tctactgatc gagcgatcgg acgccatggc caaggatata cagtcccggc
     1921 agtcgcagtt gcgtcagctc atcgcccaac aggcggcgcg catgatggcg gaagaaggca
     1981 tctcggatta cgcctatgcc aagcgcaagg caagccgcca gcttggcgcc accgatatca
     2041 actgcatgcc gaccaatgcc gagatcgaag acgagatccg cctgttccac gagatctaca
     2101 acagcgagga tcagccggcc catttgcgcc agctacgcgc agacgccttg ctggtcatgc
     2161 agttgctgga acgtttcgaa ccccatctca ccggcgccgt ctgggatggc actgccggca
     2221 agtacgcgga gacgcatatc catctgttcg cggacagcct caaggatgtc gagatgttcc
     2281 tgctgaacca gcagatccct tacgagacca acgagaagtc ctatcgggtg agcaatgctg
     2341 ccgcggacaa aaaaggaggg gaacgcagga aagtaccgat gttcacgctc gaggggccgc
     2401 atggcgcgat caaactcagc gtgttcgaga tcgacgacat gcgtatcccg accaagcacc
     2461 cgggcaacgg cagcaatgct agccgggccg atatcagtga tctcaaggcg ctgatcgctt
     2521 cgactgcggc gctcaccgag tccgaataac tcccccgcta attaagttcc ctgccgctca
     2581 agccttgagc caacgcgcga catccagggc gtaataagtc agcacgccgt ctgcaccagc
     2641 acgcttgaac gccagcatgg cttccagcac gcatgcccgt tcatcgagcc agccgttctg
     2701 tgcagccgct ttcagcatcg cgtattcacc gctgacgtga tacgcatagg tcggcactcc
     2761 aaactgcgcc ttcacccggc gcacgatatc cagataaggc atgcccggtt tcaccatcac
     2821 catgtccgca ccttcgtcga ggtccagcgc cacttctctg atcgcctcgt ctgaattggc
     2881 gggatccatc tggtagttgt acttgttgcc tttgccaaga ttggcggaag accctaccgc
     2941 gtcgcggaag ggaccataga acgcggaggc gtatttcgcc gaataagcga ggatgcgcgt
     3001 atggatatgg ccagcctgct ccagcgcctg acggatggca ccgacccgtc catccatcat
     3061 gtctgacggt gccactacgt cggcgccggc ggccgcgtgg cacaaggcct gcttgaccag
     3121 caccgcggtg gtctcatcgt tcaatacata cccactggca tcgatgatgc cgtcctgacc
     3181 atgggtggta taggggtcaa gcgccacatc ggtgatcacc cccaggtcag ggaacgcttc
     3241 tttcaatgcc cgcaccgtac gtggcaccag gccttgtgga ttgaatgcct cattcgcgtc
     3301 tagcgacttc agcgattgat cgaccacggg gaacaacgcc agcgctggga tccccaaggc
     3361 gacacattcc gccgcggtct ccagcagcac gtcgatactc tggcgctgca cacctggcat
     3421 ggatgccacg ttctccgtgc ggcgttcgcc atcaagtacg aataccgggt agatgaggtc
     3481 atcgacggtc agcacgtgct ctcgcatcag gcgccgcgaa aaatcatcct tgcgcatgcg
     3541 gcgcggtcgg ctcaggggga atttgctcat gttcagtcct tcatcttagc gccgataacc
     3601 accggcccag ctcgataaac gcacctttcg agcgagagac cggcgagtat ccgcggtcaa
     3661 tcacgtttca ggaaaatacc cgcgccagct ccaacccagg atccggctgc cgcatgaacg
     3721 cctcgccgac gaggaaggta tgcacatcat gcgaacgcat cagcgccacg tcgtcagggg
     3781 tgaagatacc ggattcggtg acgacgatct tgtcggccgg gatccgcgcc agtaaatcga
     3841 aagtcgtttg cagcgtgacc tcgaaagtgc gcaggttgcg gttgttgatg ccgagcagcg
     3901 gcgtgtccag ttgcagggcg aggtcgagct cttcgccgtt atgcacctcg accagcacgg
     3961 ccatgcctag atcatgggcg atcgcttcca gttgacgcat gcgcggcaaa tttagcgcag
     4021 ccgcgatgag caggatgcaa tccgctccca tcgcgcgcgc ttcatagact tgatacgtct
     4081 cgatcatgaa atccttgcgc aacaccggga tcgcgcaggc ggcgcgcgcc tgcttgagat
     4141 attcggcact gccctgaaag aactgctgat cggtgagcac ggacaggcag gcagcgccgt
     4201 tatcggcata gctcgccgcg atctcggccg gacgaaagtc cgctcggatc acacccttgg
     4261 acggactggc tttcttgatc tcggcaatga cggccggctt tccggcagcg atcttgcccc
     4321 ggatcgcgcc gacgaaatca cgcgccggtg gcatcgccat cgcctcacgc tgcagaatag
     4381 ccagcggctt ctcggccatt gcggcttcca cctcggcctg cttgacgttc agaatcttgt
     4441 tcaaaatatc ggacattgtt actgctaccc tcttcgaatc atcttgttac cggctattca
     4501 taagtcttac cgtcatgccg caaccagccc ggcgcgtgtt gcccgtgcgg atcatggtgc
     4561 gtccagtgga ccaccccgcc cttgtcgttc catacatatt cgccattgaa ctcgaccaca
     4621 tcgcctactg caagtcgcgg caccctgggc gcgagatcga tattgtgcgc gaccagcacc
     4681 gtgataccca ctggtagctg aaggatgaag cgctgatgcc tcgcaccgtt gccatcatcc
     4741 ggcaaaacct tgagcacttt gcccttgccg gtgacttgta tatcgctgag tcggccatcg
     4801 aacgcgtggc ggatcgcttc gatgtcacca gcggtttgtt ccgtcgcaac ggctacctgc
     4861 tgcggtagtt gtgccaaccc ggacgacggg tactgcagca ccgtatagac aatggcgatg
     4921 gcgacgatca gcaatagctt gattatgcgc tgacggcgat cgctttcagc tgctccagct
     4981 tatgtgcggc agcgccacta tcgatgacct ccgctgccag ggcgataccc gccgccatcg
     5041 attctgccac gcctgcgaca tagatcgctg cgccggcgtt gagcaatacg atgtcccgcg
     5101 ccgggcctgt cttgcctgcc aacacatcca gcaccatggc cctggatgcc tgggcgtcct
     5161 ggatacggat ggtcgtcagg gcatgtacgg gcagaccgaa ttggctcgga tggatgacgt
     5221 attcgtgtac ctcaccatgc ttcagttcag cgacataggt atccccggtg aaggagatct
     5281 cgtccatgcc gtcagcacca tgcaccacca tgacgtgttc gctgccgagg cgttgcagca
     5341 cgcgcgccag caatccggtc aggtccttgt ggaagacgcc catcacctgg cgtttggcac
     5401 cggccggatt ggtcatcgga cccagcaggt tgaacaaggt gcgcacgccc agctcgcgac
     5461 gtacaggtgc cgcgtgcttc atggcactgt ggtggttggg cgcgaacatg aagccgatgc
     5521 cgatcttgtc cacggccgtc gcgacctgct gcggggtcag gttgacgttg acgcccagcg
     5581 cttccagcac atcggcgctg ccgcaggtgg acgataccga acgcccgcca tgtttggcga
     5641 cgcgcgcgcc ggcggcggca gcaacgaaag cgctgaccgt cgagacgttg aatgtctgga
     5701 taccgtcgcc gccagtcccg caggtatcga ccagatggcc ggtgtcatgg atctcgacct
     5761 tggtcgacaa ttcgcgcatg acctgcgcgg cggcgctgat ctcatccacc gtctcgcctt
     5821 tcatgcgcaa gccgatgagc agcccagcga tctgcgcggg cgtcagttcg ccctgcatca
     5881 cctggcgcat gatggccagc atctgttcgt gcgtcagatc ctggcggttc agcaactgct
     5941 ggagcacctc cttgaaaacg accgccatca gtaggctttc aggaagttat cgagcaactc
     6001 gtggccatat tcggtgagga tcgattccgg gtggaactgc acgccttcga tcggcaaggt
     6061 cttgtgacgc acgcccatga tctcgccgaa ggcgccgttc tcgtcttcgg tccacgccgt
     6121 gatctcgaga cagtcgggga tggtctcgcg ctcgatcacc agcgaatggt aacgcgtggc
     6181 ggtataggga ttgggcaggc ccttgaacac gcctgtgtcc ttgtggtgga tctgagaagt
     6241 cttgccgtgc atgagctgct tggcatggac gatgttgccg ccaaaggcct ggccgatact
     6301 ctggtggccg agacagacgc ccagcatcgg gatcttgccg gcaaaggcct tgatcagcgg
     6361 cactgagatg ccggcctcgt tcggcgtgca tgggccggg
//
LOCUS       DATMTW010000242         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_558476, whole genome shotgun sequence.
ACCESSION   DATMTW010000242 DATMTW010000000
VERSION     DATMTW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558476"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /gene="accD"
                     /locus_tag="VN084_04755"
     CDS             complement(<1..760)
                     /gene="accD"
                     /locus_tag="VN084_04755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWT28799.1"
                     /translation="MSWLQKLLPPKINRPAGASKKNVPEGLWSKCASCDAVLYRTDLA
                     SNAEVCPKCNYHNRISARARLDLLLDEEGRFEIGAEVQPVDPLKFKDSKRYADRIKST
                     QSDVGETDSLIVMQGSIKTVPTVVAAFEFKFMGGSMGSVVGERFVRGVQTAIEQKAAF
                     VCISASGGARMQEGLFSLMQMAKTSAALTELSKAGLPYISVLTDPTMGGVSASFAMLG
                     DIIVAEPQALIGFAGPRVIEQTVRETLPDGFHRAE"
     gene            complement(779..1579)
                     /gene="trpA"
                     /locus_tag="VN084_04760"
     CDS             complement(779..1579)
                     /gene="trpA"
                     /locus_tag="VN084_04760"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWT28800.1"
                     /translation="MSRIQTTFAALKQQNKKALIPYITAGDPHPDHTVGLLHTLVKSG
                     ADMIELGVPFSDPMADGPVIQRASERALAHHVSLNKVLEMVRTFREQDSQTPIVLMGY
                     ANPIEAMGMETFVQRAKAAGVDGVLTVDYPPEECTEFVRLLQAEGMDPIFLLSPTTEP
                     ARVDVIVNQASGFVYYVSLKGVTGAKNLDIVEVADRVSDIRSKTDIPIGVGFGVRDAA
                     TAKAVAEIADAVVVGSRMVQAIEQSTEATLASDVAALMTELRSAIDTK"
     gene            complement(1599..2801)
                     /gene="trpB"
                     /locus_tag="VN084_04765"
     CDS             complement(1599..2801)
                     /gene="trpB"
                     /locus_tag="VN084_04765"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWT28801.1"
                     /translation="MQVYDMPDERGHFGPYGGIFVAETLIEALDELRAMYERYRHDAD
                     FLAEFEHDLKYFVGRPSPIYHAKRLSEHFGGAQIYLKREDLNHTGAHKVNNCIGQALL
                     AKRMGKPRIIAETGAGQHGVATATVAARMGLECVVYMGSEDVKRQAPNVYRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVTNIENTFYIIGTVAGPHPYPMMVRDFQSIIGREAR
                     VQMPDLIGRQPDAVIACVGGGSNAMGIFYPYIDDKDVRLIGVEAAGFGMDTGKHAAPL
                     TANSPVGVLHGNRTYLMQDADGQIIETHSISAGLDYPGVGPEHAWLKDIHRAEYVAVT
                     DDEAMQAFHTLCRMEGIIPALESSHALAYAAKLAPTMNKEQVLLINLSGRGDKDINTV
                     AKLSGIEL"
     gene            complement(2788..3411)
                     /locus_tag="VN084_04770"
     CDS             complement(2788..3411)
                     /locus_tag="VN084_04770"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWT28802.1"
                     /translation="MRTRVKICGITRPQDALAAVTQGCDAIGMVFYAPSPRNVSIPQA
                     QAICAVLPPFITTVGLFVDADPAWIDQVLAAVPLDLLQFHGDESPAACARPGRPYMKA
                     VRVKPGLNLIQYAADYAAAQALLLDAYAEGQPGGTGQTFDWALVPVDLPKPVVLAGGL
                     NADNVSVAIQRVRPYAVDVSGGVERSKGIKDADMIAAFMRGVSDASL"
     gene            complement(3485..4267)
                     /gene="truA"
                     /locus_tag="VN084_04775"
     CDS             complement(3485..4267)
                     /gene="truA"
                     /locus_tag="VN084_04775"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWT28803.1"
                     /translation="MRVALGIEYDGSGFCGWQSQPSGCGVQDHLESALSHIAGHALRV
                     TAAGRTDTGVHALGQVVHFDTQAERPDSAWVRGVNAFLPTSIRVQWAMQVDVAFHARF
                     CAERRSYTYWLVNQPVAPAVMASKAGWFHHPLDLTAMAEAAACLLGEHDFSAFRAAEC
                     QAKTPVKQMYEARVTRQGEYLLFDFSANAFLHHQVRNMVGALVYIGKGKYPPSHMRTL
                     LQSRDRAISPPTFSASGLYLRAVGYDGKWGLPEHQRRLALLL"
     gene            complement(4264..4881)
                     /locus_tag="VN084_04780"
     CDS             complement(4264..4881)
                     /locus_tag="VN084_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28804.1"
                     /translation="MPIHPSIRIVAIVLLAILLQRLSLLPVLVLAVAMAMLLITVRAR
                     IYLTMMRRMRWLLLVMLLVYAFTTPGEYFSSWPLWLAPTHEGLRAGLIQALRLSVMLA
                     GLALLLRLSTREQLVVGFLMLLRPLRHLGLDTERFAARLWLTLHYVEQPQLEKTPAAM
                     FQRLKTFHLDDNTEPPMTIHLHAPAFGWHDWCILFLLLVAGIATI"
     gene            complement(4931..>5191)
                     /locus_tag="VN084_04785"
     CDS             complement(4931..>5191)
                     /locus_tag="VN084_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HWT28805.1"
                     /translation="LDLGDTAAESADSPAEPFMVDAAADMTATAGSVQDESEEVNTKL
                     DLVTAYMEIGDSEGARELLDEVMKEGGPKQQARAKEILATIA"
BASE COUNT         1074 a         1557 c         1528 g         1032 t
ORIGIN      
        1 actcggcacg atggaatccg tctggcagcg tctcgcgtac cgtctgttcg atcacgcgcg
       61 gacctgcgaa gccgatcagc gcctggggtt ctgcgacgat gatatcgccc agcatggcga
      121 aactggcaga gacgccgccc atggtcggat cggtcagtac ggagatatat ggcagtccgg
      181 ctttgctcag ctcggtcagc gccgcgctgg tcttggccat ctgcatcaga gagaacaggc
      241 cttcctgcat gcgagcgcca ccactagccg agatgcagac gaacgccgct ttttgttcga
      301 tggcagtctg tacgccacgg acgaaacgct cgccgacgac cgaacccatc gagccgccca
      361 tgaacttgaa ttcgaatgca gcgacgactg tgggaacggt cttgatgctg ccttgcatga
      421 caatcaggga atcggtctca ccgacatcgg attgcgtact tttgatacgg tccgcgtagc
      481 gcttgctatc cttgaatttg agaggatcga ccggctgcac ttcagcaccg atctcgaagc
      541 gcccttcttc atccagcaac agatcgaggc gcgcgcgggc ggagatgcgg ttatggtaat
      601 tgcatttcgg acagacttcg gcattgctgg cgaggtccgt ccggtacaag accgcgtcgc
      661 acgatgcgca tttgctccag agcccctcag gcacgttttt cttactggca cccgcaggac
      721 ggttgatttt tggcggtaag agtttttgta accagctcat ggcattcccc agatgtgttt
      781 atttcgtgtc aatggcagag cgcagctctg tcatcaacgc cgcgacatcg ctcgcgagcg
      841 tcgcctcggt agactgttcg atcgcttgaa ccatacggct gccgacgacg accgcatcgg
      901 cgatctcagc gaccgcctta gcagtagccg cgtcgcgcac gccaaaaccc acgccgatcg
      961 gaatgtcggt cttgctgcga atatcactga cccggtcagc cacctcgacg atatccaggt
     1021 tcttggctcc agtcacaccc ttcagcgaaa cataatagac gaatccactg gcctggttga
     1081 cgatgacgtc gacacgtgcg ggctcagtcg tcggtgacaa caggaagatc ggatccatgc
     1141 cttcagcctg cagcagcctc acgaactcgg tacattcctc gggggggtaa tcgaccgtca
     1201 atacgccatc gacgccagct gccttggcac gctgcacgaa ggtctccata cccatcgcct
     1261 caatcggatt ggcatagccc atgagcacga tcggggtctg cgaatcctgt tcacgaaagg
     1321 tccgtaccat ctccagcacc ttgttcaggc tgacgtggtg cgcaagcgct cgttcactgg
     1381 cacgctggat caccgggcca tccgccatgg ggtcggaaaa cggcacgcct agctcgatca
     1441 tgtccgctcc ggacttgacc agcgtatgga gcaagccaac ggtgtggtcc ggatgtggat
     1501 cgccagcagt gatgtagggg atcagggctt tcttgttctg ttgcttgagt gcagcgaacg
     1561 tggtctggat gcgggacatg gtggcaatgc ttctgttctt atagttcgat gccggatagc
     1621 ttggcaacgg tgttgatatc tttatcgcca cggccggaca ggttgatcag caacacctgt
     1681 tccttgttca tggtgggcgc cagcttggcg gcataggcga gcgcatggct cgattccagt
     1741 gccgggatga taccttccat gcgacacagc gtatggaaag cctgcatggc ttcgtcatcg
     1801 gtgaccgcca catattctgc gcgatggata tccttgagcc aggcgtgttc tgggccgaca
     1861 cccggataat ccaggcccgc cgagatggag tgggtctcga tgatctgtcc atcggcatct
     1921 tgcatgagat atgtgcgatt gccatgcagc acgccgaccg ggctgttggc ggtcaggggt
     1981 gctgcatgct tgccggtgtc cataccgaaa cctgcagctt cgaccccgat caggcggaca
     2041 tccttatcgt cgatataggg gtagaagatc cccatcgcgt tggaaccacc gccgacacaa
     2101 gcgatcacgg cgtcaggctg gcgaccgatg agatccggca tctgcacgcg ggcttcacgg
     2161 ccgatgatgg actgaaaatc gcgcaccatc atcggatagg gatgtgggcc agcaacggta
     2221 ccgatgatgt agaaagtgtt ctcaatgttg gtcacccaat cgcgcatggc ttcgttaagc
     2281 gcatccttga gggtcttgga gccagactcg acaggcacca cggtggcgcc gagcagcttc
     2341 atgcgataga cgttaggcgc ctggcgcttg acgtcctcgg agcccatgta gaccacacat
     2401 tcaagaccca tacgcgcggc gaccgtcgct gtcgcgacgc catgctgtcc ggcgccggtc
     2461 tcggcgatga tccgaggctt gcccatgcgt ttggcaagca gcgcctggcc gatgcaattg
     2521 ttcaccttat gtgcgccggt atgattgaga tcttcgcgtt tcaggtagat ctgcgcgccc
     2581 ccgaaatgct cggacaatcg cttggcatga tagatggggc tggggcggcc aacgaaatac
     2641 ttgaggtcgt gctcgaactc ggcaagaaaa tcggcatcat ggcgatagcg ttcatacatc
     2701 gcacgcaact cgtcgagcgc ctcgatcagg gtctcggcga caaaaatgcc gccatagggg
     2761 ccgaaatgac cgcgctcatc cggcatatca taaacttgca tcactcactc ctcgcatgaa
     2821 tgcggcgatc atgtccgcat ccttgattcc tttggatcgt tctacgccac cgctgacatc
     2881 taccgcatag ggcctgactc tttgaatcgc cacactcacg ttgtcggcat tgaggccgcc
     2941 ggcgagcacg accggcttcg gcagatcgac cggaaccaga gcccagtcga aagtctgtcc
     3001 ggtacccccg ggctgacctt ccgcatatgc gtccagcaac agcgcctgtg cggcggcata
     3061 atccgccgca tattgtatca aatttaagcc gggcttgacg cgtaccgcct tcatataagg
     3121 acggccaggg cgggcacatg ccgcgggact ctcgtcgccg tggaactgca ggagatcgag
     3181 cggtacggcc gccagcacct gatcgatcca ggccggatcg gcatccacaa acaggccgac
     3241 ggtcgtgatg aaaggcggca gcaccgcgca gatggcttgc gcctgaggga tgctgacgtt
     3301 acgtgggctg ggggcgtaga acaccatgcc aatggcgtca cagccttgtg tgactgcagc
     3361 cagggcatcc tgggggcggg tgatcccgca aatcttgact cgggttcgca tgatttcgga
     3421 gttggcggat gaggcgcttg aaaacaagcg gctagcgtaa cgtaaacccc gccggctgtc
     3481 acattcaaag tagcagcgct agccgccttt gatgctctgg cagcccccac ttgccatcgt
     3541 agcctactgc ccgcagatac aagccactgg cagagaaggt tggcggggat atcgcacgat
     3601 cccggctttg cagtagcgtt cgcatgtggc tgggcgggta tttgcccttg ccgatataga
     3661 ccagcgcacc gaccatgtta cgcacctgat ggtgcaggaa ggcattggcg ctgaaatcga
     3721 atagcagata ctcgccctgc cgggtgacgc gcgcctcgta catctgtttg accggtgtct
     3781 tcgcctggca ctcggccgcg cgaaatgcgc tgaagtcatg ttcgcccagc aggcaggctg
     3841 cagcttctgc catggctgtc aggtccagcg gatgatggaa ccagccagcc ttgctcgcca
     3901 tgacagctgg tgccacgggc tgattgacca gccaataggt atagctgcga cgctcggcgc
     3961 aaaaacgggc atggaacgcg acatcgacct gcatcgccca ttgcacacgt atagacgtgg
     4021 gcaagaatgc gttgacccca cggacccacg cgctatctgg ccgctccgcc tgcgtatcga
     4081 agtgcaccac ctgcccgagc gcgtggaccc cagtatcggt acgcccggcc gctgtcaccc
     4141 gcaacgcatg tccggcgatg tgcgaaagcg cggactccag gtgatcctgc acgccacagc
     4201 cactgggctg actctgccag ccacagaagc cgctaccgtc atattcgatg ccgagggcga
     4261 ccctcatatg gtcgcgatcc cggccacgag caacaggaac aggatgcacc agtcatgcca
     4321 gccgaatgca ggcgcatgca gatggatcgt catcggcggc tcggtgttgt catccagatg
     4381 gaaggtcttc aggcgttgga acattgcggc gggcgtcttt tccagttgcg gctgctcgac
     4441 atagtgtaac gtcaaccata gtcgggcggc gaaacgctca gtgtctaatc ccaggtgacg
     4501 taacggccgt agcagcatca ggaatcccac aacgagctgc tcccgcgtgg acaggcgcaa
     4561 cagcaaggca agccctgcca gcatgacgga caggcgcaat gcctgtatca gacccgcacg
     4621 caatccctcg tgagtgggcg cgagccagag cggccagctc gagaaatatt caccaggggt
     4681 ggtgaaggca tagaccaaca gcatgaccag cagcaaccag cgcatacggc gcatcatcgt
     4741 cagatagatc cgtgcgcgga cggtgatgag cagcatggcc atcgctactg ccagaaccag
     4801 cacaggcagc agcgacaacc tctgtagcag gatggccagc aggactatgg caacaatgcg
     4861 gatggaagga tgtatgggca tcaacaaaaa agccgggcat caagcccggc tggaatcgat
     4921 gacgatctcg ctaggcgatc gtcgccagga tctccttggc acgtgcctgc tgtttcgggc
     4981 caccctcttt catgacctcg tccagcagtt cgcgcgcccc ctcgctatcg ccgatctcca
     5041 tgtaggctgt taccagatcg agcttggtgt tcacctcttc ggactcatcc tgtaccgaac
     5101 ccgccgtagc cgtcatgtca gccgcagcgt cgaccatgaa aggttcggct ggcgagtccg
     5161 cggattcagc agccgtgtcg ccgaggtcaa g
//
LOCUS       DATMTW010000243         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_659596, whole genome shotgun sequence.
ACCESSION   DATMTW010000243 DATMTW010000000
VERSION     DATMTW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659596"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1072
                     /gene="mtnA"
                     /locus_tag="VN084_04790"
     CDS             2..1072
                     /gene="mtnA"
                     /locus_tag="VN084_04790"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWT28806.1"
                     /translation="MDGIAYRTIWPDSRLKCVHIIDQTSLPHRFEIRQIHSVEAMEYA
                     IKTMQVRGAPLIGAAAAYGVALAMLYDDSDASLQNACDDLLASRPTAVNLRWAVQRML
                     QHLMPLEPRRRYLNAWKEAAAICEEDAAQNEAIGRHGLPLIREIAVRKSDTVNILTHC
                     NAGWLATVDWGTALSPIYAAHDAGIQVHVWVDETRPRNQGASLTAWELGKHGVPHTVI
                     ADNAGGHLMQHGEVDMVIVGADRVTANGDVCNKIGTYLKALAAHDNQIPFYAAVPSPT
                     IDWTLHDGMQIPIEERSPNEVAYMQGQLPNGELASVRVVPEGSACANPAFDTTPARLV
                     TGIITERGVCPPAALPGLFQGG"
     gene            1076..>1660
                     /locus_tag="VN084_04795"
     CDS             1076..>1660
                     /locus_tag="VN084_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWT28807.1"
                     /translation="MLKEREQLLHITRKLAESGLNRGTSGNASVRVSEGFLVTPSGME
                     VEAMTPHDMVWMDGKGQFHGERQPSSEWRFHHDILKARPEIGAVIHTHSMFATTMACL
                     QRGIPPFHYMIALTGADTIRCAPYALFGSQALSDHAVAALQGSKACLLANHGMIVLGE
                     DLEKAYAVAVEVETLCEQYWRALQVGEPALLTADQ"
BASE COUNT          332 a          511 c          519 g          298 t
ORIGIN      
        1 catcgacggc atcgcttatc gcaccatctg gcctgattcc cgactcaagt gcgtgcatat
       61 catcgaccag acttcattgc cccatcgttt cgagataagg caaatccata gcgtagaggc
      121 gatggaatat gccatcaaga ccatgcaggt gcgaggtgcc ccgctgatcg gcgccgccgc
      181 agcttatggg gtggcgctgg ccatgctcta tgacgatagc gacgccagtc tgcaaaatgc
      241 ttgtgacgat ctgctcgcca gcagacccac ggcagtcaat ctgcgatggg cggtgcagcg
      301 tatgctgcaa catctgatgc cgctggagcc tcgtcggcga tacctcaatg cctggaaaga
      361 agcggccgcg atatgtgaag aagacgcggc gcagaacgag gccatcggcc ggcatggcct
      421 gcccttgatt cgcgagatcg cagtgcgcaa gtcggacacg gtgaatatcc tcacgcattg
      481 caatgccggt tggctggcga cggtggactg gggcaccgcc ttatcaccga tctatgcggc
      541 ccatgacgct ggcatccagg tccatgtctg ggtggacgag acccgcccac gcaaccaggg
      601 cgccagcctc acagcctggg agctcggcaa gcacggcgtg ccgcacaccg tgatcgccga
      661 caatgctggc ggccacctga tgcaacacgg cgaggtggat atggtcatcg tcggcgccga
      721 ccgcgtgacg gcgaacgggg acgtctgcaa caagatcggc acttatctca aggcgctggc
      781 agcgcatgac aaccagatcc ccttttacgc agcggtgcct tctccgacca tcgactggac
      841 cttgcatgac gggatgcaga tccccatcga ggagcgcagc ccgaatgaag tggcctacat
      901 gcaggggcag ctaccgaacg gcgagctggc aagcgttcgc gtcgtgccgg agggcagcgc
      961 ctgcgccaac cctgcgttcg ataccacgcc agcccggctg gtgaccggca tcatcactga
     1021 acgcggcgtg tgtccgccgg ctgcattgcc gggcctattc cagggaggtt gatccatgct
     1081 caaggaacgc gaacaactgt tgcatatcac gcgcaagctc gccgaatccg gccttaatcg
     1141 cggtacctcc ggcaacgcca gcgtgcgggt cagcgagggt tttctggtca cgccttccgg
     1201 catggaagtc gaggcgatga cgccgcatga catggtatgg atggacggca agggccaatt
     1261 tcatggcgag cgccagccgt cgagcgaatg gcgcttccat cacgatatcc tcaaggcgcg
     1321 gccggagatc ggcgccgtca tccatacgca ttccatgttc gcgaccacca tggcctgcct
     1381 gcagcgcggc atcccgccgt tccattacat gatcgcgttg acaggcgctg acactatccg
     1441 ttgcgcgcca tacgcgctat tcggctcgca ggcgctctcc gatcacgcgg tggccgcatt
     1501 gcagggcagc aaggcttgcc tgttggccaa tcacggcatg atcgtgctgg gagaggatct
     1561 ggaaaaggcg tatgccgtcg cagtggaagt ggagaccttg tgcgagcagt actggcgtgc
     1621 attacaggtg ggggagccgg ctctgctcac ggcagaccag
//
LOCUS       DATMTW010000244         4591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_89136, whole genome shotgun sequence.
ACCESSION   DATMTW010000244 DATMTW010000000
VERSION     DATMTW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89136"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /gene="fdxA"
                     /locus_tag="VN084_04800"
     CDS             complement(<1..322)
                     /gene="fdxA"
                     /locus_tag="VN084_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HWT28808.1"
                     /translation="MTYVVTENCIQCKYTDCVDVCPVDCFVEGPNFLAINPDECIDCT
                     LCVAECPAEAIFAEDDVPADQQHFIELNARLSQVWPAITSKKDALPDADANNGRSGKL
                     AELVE"
     gene            complement(422..1702)
                     /gene="cysN"
                     /locus_tag="VN084_04805"
     CDS             complement(422..1702)
                     /gene="cysN"
                     /locus_tag="VN084_04805"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HWT28809.1"
                     /translation="MTGQAHNDSLLRFMTCGSVDDGKSTLIGRLLYDTKTILADTLTQ
                     IEKTSKKRGMEAVDLSLLTDGLQAEREQGITIDVAYRYFSTGTRKYIIADAPGHEQYT
                     RNMVTAASTANLAIILIDARKGVLTQTRRHSFLAHLVGIPHIVVAVNKMDLVDYDQAV
                     FDKIRADYLAFATEIGLAQARDIQFVPLSALNGDMLVDRGDAMPWYSGPTMLEILEDA
                     PPAHTEKNEAFRFPVQFVCRPHASDDPELHDFRGFMGRIESGEIAVGDSVTVLPSGLS
                     SKVKAIMIGDQNLQRAITEQSVTLLLEDEIDTSRGDMIVKSGQVPEPVKQIDAMVCWL
                     SETPLSAARTYIIRHTTRESKAKVGEISYKIDVNTLEQLPAQGLAMNDIARISFRLAQ
                     PIMADAYSENRATGAFIVIDESTNNTVGAGMITR"
     gene            complement(1728..2663)
                     /gene="cysD"
                     /locus_tag="VN084_04810"
     CDS             complement(1728..2663)
                     /gene="cysD"
                     /locus_tag="VN084_04810"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWT28810.1"
                     /translation="MTTLTKPTLSHLDWLEAEAIHILREVAGQCSNPVLLFSGGKDSL
                     CILRLAEKAFRPGRFPFPLMHIDTGHNYQEVVNFRDRRAAELGERLIVRSVEDSMQRG
                     SVVLKSPDEPRNKHQSVTLLEAIAEFGFDCCIGGARRDEEKARAKERIMSFRDEFGQW
                     DPKNQRPELWNLYNARSHKGENIRAFPISNWTEMDVWQYIEREQLELPSIYFAHQRDV
                     VMRDGAIFPVNVPLASGQVINQPKTGEEVINMQVRFRTVGDITCTAPVLSDADSTEKI
                     VLETATTTITERGATRLDDQTSDASMEQRKKEGYF"
     gene            complement(2711..3484)
                     /locus_tag="VN084_04815"
     CDS             complement(2711..3484)
                     /locus_tag="VN084_04815"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWT28811.1"
                     /translation="MSDTVSPLILKPNANNSVNRPEMTDALRQSITAKRDAVEKLLAD
                     AVAEFGGRGTITFANSMGAEDMVLTDIILSERLPIEIFSLDTGRLPVETYDLIAEVER
                     RYKTRLKLYFPLAENVETYVQQHGINAFYESIDLRKACCHMRKVEPLGRALKGKKAWI
                     TGMRAQQAATRNALPVREFDSNNGIEKFNPLADWTEQEVWGYIRNRDIPYNKLHDIFY
                     PSIGCAPCTRAIAMGEDVRAGRWWWEDPTSKECGLHVKK"
     gene            complement(3481..4098)
                     /locus_tag="VN084_04820"
     CDS             complement(3481..4098)
                     /locus_tag="VN084_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HWT28812.1"
                     /translation="MSKSNLIQNGQIVADEWQTVQLPEIGEKIVRKQAGKVVMFKLTG
                     EVTTTAEQVSATVIPDSGKVILPLSVWLARKDALADRIAKGEIGVWLATHEVLETLTE
                     AQADLNVLPIIAVYVERFADGRIFSIGNLLRTRHGYKNELRAFGDVLRDQLFFLKRCG
                     YDSYLIRADRSAEDALASLKDFTQPYQGAVDINQPVWRRFNRTVA"
     gene            complement(4091..>4591)
                     /locus_tag="VN084_04825"
     CDS             complement(4091..>4591)
                     /locus_tag="VN084_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWT28813.1"
                     /translation="VWEIARANGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAI
                     QMQFDNLDYLYDIGELELNISGCMNACGHHHIGHIGILGVDKDGSEWYQVTIGGKQGH
                     DASLGTVIGPSFSADEMPGVVQRLIEVYLAQRTPEERFIDTARRIGVAPFKEHVYAGR
                     KEAAHV"
BASE COUNT          829 a         1277 c         1463 g         1022 t
ORIGIN      
        1 attccaccag ctcggccagt ttgccgctac ggccattgtt ggcgtcggcg tcaggcagtg
       61 cgtctttctt gctggtgatg gccggccata cctgcgagag acgggcattc aattcgatga
      121 agtgctgctg gtccgcaggc acgtcgtctt ccgcgaagat cgcctccgcc ggacactcgg
      181 cgacgcacaa ggtgcagtcg atacattcgt ctggattgat cgccaggaaa ttggggcctt
      241 ctacaaagca atccaccggg cagacatcga cgcagtcggt gtatttgcac tggatgcagt
      301 tttcggtcac tacataagtc atattcgagt cctcttcatg ctttgtatga atcgcaaagc
      361 gtcattttaa tggaattcgg ccgctaatta catctgcggc gcatacatct accggatata
      421 tctaccgtgt gatcatgccg gcaccgaccg tattgttggt cgattcatcg atgacgatga
      481 acgcgccggt cgcacggttc tcgctgtaag cgtcagccat gatcggctgt gccagacgga
      541 agctgatgcg agcgatgtcg ttcatggcca gaccctgggc gggcaattgc tccagcgtgt
      601 tgacgtcgat cttgtaggag atctcgccga ccttggcttt cgattcgcgt gtggtatggc
      661 gaatgatgta cgtgcgggcc gccgagagcg gggtctcgga cagccagcag accatggcgt
      721 cgatctgctt taccggttcc gggacttggc cggacttgac gatcatatcg ccgcgcgagg
      781 tgtcgatctc gtcttccagc agcagtgtca ccgattgttc ggtgatggcg cgctgcaagt
      841 tctggtcgcc gatcatgatg gccttgacct tggagctaag accagacggc agtactgtca
      901 cgctgtcacc gacggcgatc tcgcccgatt cgatacggcc catgaagccg cggaagtcat
      961 gtagctccgg gtcatccgac gcgtgcggac ggcagacgaa ctgcacgggg aagcggaagg
     1021 cttcgttctt ttcggtgtgc gcgggcggcg catcctcgag gatctccagc atggtcgggc
     1081 cgctgtacca aggcatggca tcgccgcgat cgaccagcat gtcgccgttg agggctgaca
     1141 gggggacgaa ctggatgtca cgtgcctgag cgagcccgat ctcggtggcg aacgccaggt
     1201 aatcggcgcg gatcttgtcg aacaccgctt gatcgtaatc caccagatcc atcttgttga
     1261 cggcgacgac gatatgcggg atgccgacca gatgcgccag gaaggaatgg cgacgcgtct
     1321 gcgtcagcac gcccttgcgc gcgtcgatca ggatgatggc gagattggcg gtggaagcgg
     1381 cggtcaccat gttacgggtg tactgctcgt gcccgggggc atcggcgatg atgtacttgc
     1441 gcgtgccggt gctgaaatag cgataagcga cgtcgatggt gatgccttgc tcgcgttcgg
     1501 cttgcaggcc gtcggtcagc agggagaggt cgaccgcttc catgccgcgc ttcttcgagg
     1561 tcttctcgat ctgggtcagc gtatcggcga ggatggtctt ggtgtcgtat agcaggcgac
     1621 cgatcagcgt gctcttgccg tcatcgacgc tgccacaggt catgaagcgc agcaggctat
     1681 cgttatgtgc ttgtccggtc atggggagtt gttccagttt cgacatctta gaaataacct
     1741 tccttcttgc gctgttccat cgaagcgtcc gaggtctggt cgtccagacg cgtcgcgccg
     1801 cgttcagtga tggtggtggt ggcggtttcc agcacgatct tttcggtgct gtccgcatcg
     1861 ctcaacacag gcgcggtgca ggtgatgtcg ccgacggtgc ggaagcgcac ctgcatgttg
     1921 atgacttctt caccagtctt gggctgattg atgacttggc cgctcgctag cggcacgttc
     1981 accgggaaga tcgcgccatc gcgcatgacc acgtcgcgct gatgcgcgaa atagatgctt
     2041 ggcagctcca gctgttcgcg ctcgatgtat tgccagacat ccatctcggt ccagttggag
     2101 atggggaagg cgcggatgtt ctcgcccttg tgcgaacggg cgttatagag gttccacagc
     2161 tccgggcgct ggttcttggg atcccattgg ccgaactcgt cacggaagct catgatgcgt
     2221 tccttcgcgc gcgccttctc ttcgtcgcgg cgtgcgccgc cgatgcagca gtcgaagccg
     2281 aattcggcga tggcttcgag cagggtcacg gattgatgct tgttacgggg ttcatcaggg
     2341 gatttgagca cgacgctacc gcgttgcatg gaatcctcca ctgaccgtac gatcagtcgc
     2401 tcgcccagct cggctgcgcg gcgatcacgg aaattcacga cttcctggta gttgtgacca
     2461 gtatcgatat gcatcagcgg gaacgggaaa cgacccgggc ggaacgcttt ttctgccagg
     2521 cgcaggatgc acagcgaatc cttgccgccg gagaacagca acacagggtt cgagcactgg
     2581 ccggcgactt cgcgcaggat gtggatcgcc tcagcctcta gccagtcgag gtgggacagg
     2641 gtgggtttgg tcaaagtggt catgagcgag cttgcgttcc aatcagatcg tatgttcggt
     2701 ggtagtccgt ctatttcttc acgtgcaggc cgcattcctt ggaggtgggg tcttcccacc
     2761 accagcggcc ggcacgcacg tcttcgccca tggcgatggc acgcgtacaa ggcgcgcaac
     2821 cgatgctagg gtagaagatg tcatgcagtt tgttataggg gatatcgcgg ttgcggatat
     2881 agccccagac ttcctgctct gtccagtcgg cgagcgggtt gaatttctcg atgccattgt
     2941 tgctgtcgaa ctcgcgtacc ggcaatgcgt tacgcgtggc tgcctgttgg gcgcgcatgc
     3001 cggtgatcca ggccttcttg cccttgaggg cgcgacccag cggttcgacc ttgcgcatat
     3061 ggcagcaggc cttgcgcaga tcgatcgatt catagaacgc gttgatgcca tgctgctgca
     3121 cataggtttc gacgttctcg gccagcggga aatacagctt gagcctggtc ttgtaacgac
     3181 gctccacttc ggcgatgaga tcgtaggtct cgaccggcag gcgaccggtg tcgagcgaaa
     3241 agatctcgat cggcaggcgc tcgctgagga tgatgtcggt cagcaccatg tcctcggccc
     3301 ccatgctgtt ggcgaaggtg atcgtgccgc ggccgccgaa ctcggcgacg gcatcggcca
     3361 acagcttttc tactgcatcg cgtttggcag tgatgctctg gcgcaatgca tccgtcatct
     3421 cagggcggtt gacggagttg ttggcgttgg gcttgagaat taacggcgat acggtatcgc
     3481 tcatgctacg gtcctgttga agcggcgcca aaccggctgg ttgatgtcga ccgcaccctg
     3541 gtagggctgg gtgaaatcct tgaggctggc cagcgcatcc tcggccgagc gatcggcacg
     3601 gatcagatag ctgtcatagc cacagcgctt gaggaagaac agctggtcgc gcaatacgtc
     3661 gccaaaggcg cgcagttcgt tcttgtagcc atggcgcgtg cgcaacaggt tgccgatcga
     3721 gaagatgcgg ccatcggcga agcgctcgac gtacacggcg atgatgggca ggacattgag
     3781 gtcggcctgg gcttcagtca gcgtttccag tacctcgtgg gtcgccagcc agacgccgat
     3841 ctcgcctttg gcgatgcggt cggccagcgc atccttgcgc gcgagccaga cggacagcgg
     3901 caggatgacc ttgccactgt cggggatcac cgtggcggag acctgttcgg cagtggtggt
     3961 gacttcaccc gtgagcttga acatcacgac tttaccagcc tgcttgcgga cgatcttctc
     4021 gccgatctcc ggcagttgga cggtctgcca ttcgtcggcg acaatctggc cattttgtat
     4081 gagattggat ttagacatgg gctgcctcct tcctgccggc atagacatgt tccttgaatg
     4141 gcgcgacacc gatacgacgc gcggtatcga tgaagcgttc ttctggcgtg cgttgcgcca
     4201 ggtagacctc gatcaggcgt tgcaccacgc ccggcatctc gtctgccgag aacgatgggc
     4261 cgatgactgt gccgagactg gcgtcatggc cttgcttgcc gccgatggtg acctggtacc
     4321 actcggaacc gtccttatcg acgccgagga tgccgatatg gccgatatgg tggtgaccac
     4381 aggcattcat gcagccagag atgttgagct cgagctcccc aatatcgtaa agatagtcga
     4441 ggttgtcgaa ctgcatctgg atggcttcgg cgatggggat actcttggcg ttcgccaggc
     4501 tgcagaagtc gccgccgggg cagcagatga tgtcggtcag caagccgatg ttgggggtgg
     4561 cgaggccatt ggcacgtgcg atctcccaga c
//
LOCUS       DATMTW010000245         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_357830, whole genome shotgun sequence.
ACCESSION   DATMTW010000245 DATMTW010000000
VERSION     DATMTW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357830"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VN084_04830"
     CDS             <1..885
                     /locus_tag="VN084_04830"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWT28814.1"
                     /translation="TVWNAIQEAEALGVEIFSVRGRGYRLPQPIKLLEYESVLHAIGE
                     QRAWFTLELHDHLESTNSYLMKKAAAGAPHATCVAASLQTKGRGRRGRSWQSGLGASL
                     TFSLLWRFQSGASALSGLSLAVGVALMRALHGLGIKRAQLKWPNDILCDQRKLAGILI
                     ELQGDMEGPSAAVIGIGLNLHLPEHVLKHIDQAAVDIHSLAPAPIDPSVLLGVLLKHL
                     AEVLSEFENRGFEHLREEWVRNHAYQQQQVRLLLPDGRELLGVVNGIAPDGVLLVDTA
                     SGQQRFSAGEISLRGVRA"
     gene            915..1688
                     /locus_tag="VN084_04835"
     CDS             915..1688
                     /locus_tag="VN084_04835"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWT28815.1"
                     /translation="MGPLLAIDCGNTRTKWGVFDAAGELKTHGVSATDALSTLQPSPA
                     WAGCRRAVISNVAGADAKAALDRLLDAMEIRYIQPGQQACGVQNRYRVPAQLGADRWA
                     ALIAAWHRYHGHCVVVSAGTAVTIDALAPDAETGEGVFLGGLILPGLQLMQHSIADKA
                     PGVKLGEGVLKDFPDNTADALYSGGLHAIAGAVRSLTECLAKRITPHSDASPRIIIAG
                     GDGEALHALLTQDAALANHLAIADNLVLQGLLILESEMR"
     gene            1685..2359
                     /locus_tag="VN084_04840"
     CDS             1685..2359
                     /locus_tag="VN084_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWT28816.1"
                     /translation="MKLLVWVLVLLNVLLFAYFNLPTHDTRVNLVSHKPIEPEKIKLL
                     TEDEVKAMPRKAADAPEAQAVAAAPPPQFACYEWGSFSAKNLPRARNILTKFSVEYTT
                     RQQNSQEAARWWIYIPRLASVQAAQAKVAELKALGVEDMFVVQEPQWKNVISFGVFKD
                     EQLATKLLEELKAKGVVSAVNGVRNQEKGQSSLLINKMSSDIAIEIDRLKPDFPGSEL
                     KQVSCQ"
BASE COUNT          527 a          716 c          799 g          474 t
ORIGIN      
        1 acggtgtgga acgccatcca ggaagcggaa gcgctcggcg tggagatctt ctcggtgcgc
       61 ggccgcggtt accgcctgcc gcagccgatc aaactgctgg aatacgagag cgtgctgcac
      121 gccatcggcg agcaacgcgc atggttcaca ctcgagctgc acgatcatct ggaatcgacc
      181 aacagttacc tgatgaagaa agccgctgct ggcgcgccgc acgccacctg tgtggctgca
      241 tcgctgcaga ccaagggccg cggacgacgc ggacgcagct ggcaatccgg cctcggcgcc
      301 agcctgacgt tctcgctgtt gtggcgcttc cagtccggtg catccgcgct ctccggcctc
      361 agcctggcag tgggggtggc gctgatgcgc gccttgcatg gtttgggtat caagcgcgcg
      421 cagcttaaat ggccgaacga tatcctctgc gatcagcgca agctggccgg catcctcatc
      481 gaactgcagg gcgacatgga aggcccgagc gccgcggtga tcggtatcgg cctcaacctg
      541 catctgccgg aacatgtgct caagcatatc gaccaggcgg cagtggatat ccatagcctg
      601 gcgccagcac cgatcgatcc gagcgtgctg ctgggcgtgc tgctcaaaca tctggcggaa
      661 gtgctcagcg agttcgagaa tcgcggcttc gaacatctgc gcgaggaatg ggtgcggaac
      721 cacgcctacc agcaacaaca ggtgcgcctg ctgttgccgg atggccgtga gttgctgggt
      781 gtggtcaatg gcatcgcgcc cgacggcgtg ctgctggtgg ataccgccag cggccaacag
      841 cgcttttctg caggcgagat cagcctgcga ggtgtgcgtg cttgagtaac gcaaagcata
      901 caacggacaa aacgatgggg ccgttgctcg ccatcgactg cggcaatacg cgcaccaagt
      961 ggggcgtgtt cgacgccgct ggcgagctca agacgcatgg cgtgagcgct accgatgcgt
     1021 tgtcgacctt acagccctcg ccagcctggg ctggctgccg gcgtgcagtg atctcgaacg
     1081 tggccggtgc cgacgccaag gcggcgctcg atcgcctgct ggatgcgatg gagattcgat
     1141 atatccagcc tggccagcaa gcgtgcggcg tgcagaaccg atatcgtgtg ccggcacagc
     1201 ttggtgcaga ccgctgggcc gcgctgatcg cggcctggca ccggtatcat ggccattgtg
     1261 tggtggtgag tgccggtact gcggtcacga tcgacgcgtt ggcacctgac gcggagactg
     1321 gtgaaggcgt tttcctcggc ggcttgatcc tgcccggttt gcaattgatg cagcacagca
     1381 tcgccgacaa ggcgccgggc gtgaagctgg gcgagggagt gctgaaagat tttcccgata
     1441 acacggccga tgcgctgtat agcggcggtc tgcacgcgat cgccggcgcc gtacgcagcc
     1501 taacggaatg cctggcaaaa cgtattacac cccattcgga tgcgtcgccg cgcatcatta
     1561 tcgccggtgg tgacggcgaa gcgctgcatg ccttgctgac gcaggatgcg gcgttggcca
     1621 atcatctggc aatcgcggat aatctggtgc tgcaaggctt attgatcctg gaaagcgaga
     1681 tgcgatgaaa ctgttggttt gggtgctggt gttgctgaat gtgctgttgt tcgcttactt
     1741 caatctgccc acgcacgaca ccagagtgaa cttggtcagc cataagccga tcgaaccaga
     1801 gaagatcaag ctgctgacag aagacgaagt caaggccatg ccgagaaagg cagccgatgc
     1861 gccggaagcg caggcggtcg ccgccgcgcc gccaccccaa ttcgcctgtt acgagtgggg
     1921 tagcttttct gccaagaact tgccgcgtgc gcgcaatatc ctgaccaagt tctcggtgga
     1981 atacaccacc cggcagcaaa actcccagga agccgcccgc tggtggatct acatcccccg
     2041 tctggcgtcc gtacaagcgg cccaggccaa ggtggccgag ctgaaagccc tcggcgtcga
     2101 agatatgttt gtggtgcagg aaccacaatg gaagaacgtg atttcgttcg gagtctttaa
     2161 ggatgagcaa ttagcgacta aactgcttga agagctcaag gcgaaaggcg tagtctctgc
     2221 cgtcaatggt gtcaggaatc aggaaaaagg tcagtccagc ttgctgatca acaagatgtc
     2281 atccgatatc gcaatcgaga tcgatcgatt gaaaccggat tttccgggta gtgaactcaa
     2341 acaggtaagt tgccaataag aaagggtaca tcatgagttt cttcaagaac attctgaaaa
     2401 aagtagcgct ggccgttgcc gtcgtcgccg gtaagaaagt cctggccaag gtgatggata
     2461 acaacgccaa accggccagt cgcgcgagcg ctgccagtaa gcccgccgcg gcgaag
//
LOCUS       DATMTW010000246         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_256121, whole genome shotgun sequence.
ACCESSION   DATMTW010000246 DATMTW010000000
VERSION     DATMTW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256121"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..2208
                     /locus_tag="VN084_04845"
                     /pseudo
     CDS             49..2208
                     /locus_tag="VN084_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831362.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
     gene            complement(2296..3066)
                     /locus_tag="VN084_04850"
     CDS             complement(2296..3066)
                     /locus_tag="VN084_04850"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HWT28817.1"
                     /translation="MSSPNLDTRKLNALHGWKWLADGFAMFRRSPVIWIALLFIYLLI
                     GMVLSTIKLIGPIVLNLLAPVFVAGFMIGCRALEKGEELEIAHLFAGFKHHTAQLITV
                     GGIYLAGAIVVVGIIFAGSDQAVLQAMVSGQKLTPEQASAALNDGFLLTMLVGLGLML
                     PLVMAYWFAPVLVAFHEMSGLQAMKQSFLACLRNLLPFFIYSVIATILLVLAALPLFL
                     GMLVMIPTMIASLYASYRDIFVVPVSEAEAAALDADEA"
     gene            complement(3083..4030)
                     /gene="thrB"
                     /locus_tag="VN084_04855"
     CDS             complement(3083..4030)
                     /gene="thrB"
                     /locus_tag="VN084_04855"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWT28818.1"
                     /translation="MSVFTTVTTEELQQWLKAYPLGELRELKGIAAGITNTNYFVTTD
                     SGRYVLTLFERNAADELPYFLDLMAHLSDHGVPCPHPVADERGHSLGALNGKPAALVT
                     CLRGKDVAQPSLAQCEAVGRVLAQLHLAGQSFGQHMDNPRDKAWRKATADSVMRFLNE
                     ADQRMLQAELDYEASLDLGALPHGVIHADLFRDNVLFDGDALGGLIDFYYACNDVLLY
                     DVAIAANDWCVAAGGTFDQPRLQALLRAYHGARPFTAAECEAWPSLLRMAALRFWLSR
                     LYDLHYPQDGELTHAKDPQHFQQILKLRIQQPTTMKLDA"
     gene            complement(4069..4410)
                     /locus_tag="VN084_04860"
     CDS             complement(4069..4410)
                     /locus_tag="VN084_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HWT28819.1"
                     /translation="MRLLRTMIVLALSLPVAAFAAESDPKLEPLPDVPPPPGVVDGDP
                     AAEPAITIIKKGEDTVEEYRINGELYMMKVTPPHGVPYYLMKEDQDSGWSRMEGPTAP
                     LVIPKWVLFNF"
     gene            complement(4540..>4710)
                     /locus_tag="VN084_04865"
     CDS             complement(4540..>4710)
                     /locus_tag="VN084_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWT28820.1"
                     /translation="DWFRDTLVTEGMVSPEDMDLVQVIDEPAKVVEAIFKHYETRGFE
                     PSAAERQIQLYL"
BASE COUNT         1032 a         1450 c         1369 g          859 t
ORIGIN      
        1 ccgattttcc gtccgttctc ccgcagccag catgcgataa taagcagcat gaatacacct
       61 gccagttccg attccctgct ttccggcctc aatgacaaac agctcgaagc cgtaaccttg
      121 ccgcatcagt ccgcgctcat cctggcaggc gcaggcagtg gcaagacgcg cgtgctgacg
      181 acgcggatcg cctggctcat ccagaccggt caggtctcgc cgcatgggct cctcgcagtc
      241 accttcacca acaaggccgc caaggagatg ctcgcgcgcc tgtcggccat gttgccgatc
      301 aacacgcgcg gcatgtgggt cggcaccttc cacgggctat gtaatcgcct gttgcgcgca
      361 caccatcgcg aggccatgct gcccgcgact ttccagatcc tcgatacggc cgaccagctt
      421 tccgcaatca agcgcgtcat gaaagccatg aatatcgacg acgagaaatt cccgccgaaa
      481 caggtcatgg gctacatcaa cagttgcaag gaagaaggcc tgcgcgcgca tgcggtggat
      541 gcctatgacg cccattcgca gcgcatgcga gaagtcttcg aggaatacga caagcaatgc
      601 cagcgcgaag gcgtcgccga cttcgccgaa ctgctgctgc gctgctatga gttgctgagt
      661 cgggagggcc atatccgcca gcattaccag agccggttca aatatatcct cgtcgacgag
      721 ttccaggata ccaaccggtt gcaatatctc tggctgaaac tcctcgcagg tagcgagaat
      781 tgcgtgttcg ccgtcggtga cgacgaccag tcgatctatg cctttcgcgg cgcgcgcgtt
      841 ggcaacatgc gcgattttga acaggatttc gcggtcgaga acatcgtcaa gctggaacag
      901 aactaccgct cgcacagcaa catcctcgac gccgccaacg ccatcatcac gcacaaccgc
      961 aaccgcctgg gcaagaatct ctggaccgcc gccggcaagg gcgagccagt acgcgtctac
     1021 caggcctata acgacatcga cgaggccaat ttcatcgtcg aggaagtgaa gtcgctgcat
     1081 gcctaaggca ctgacctgcg cgagatcgcc ctgctctatc gctccaacgc acaatcgcgc
     1141 gtgctcgagc acgccctgtt cacggccggc ctaccctatc gcgtgtatgg cggcctgcgc
     1201 ttcttcgagc gtgccgagat caagcatgcg ctggcctata tgcggctcat cgccaacccg
     1261 gatgacgata ccgctctgct gcgcgtcatc aactttccgg cgcgcggtat cggcgcacgc
     1321 agcctggaac aactgcagga agtagcgcgg acccagaaca gcagcctgtg gcaagccgcc
     1381 atgcaaagcg caggcaagat cgccgctttc gtcgcgctca tcaagcaaat gcaggaagaa
     1441 gccatcggca tcagcctgcc ggaggtagcc gaactggcga ccacgctgtc cgggctgaag
     1501 gccttctatc agaccgaaaa ggaaggcgaa gaccgcgtcg ccaacctgga agaactcgtg
     1561 tccgcagcca ccgcgttcat gaatcaggat atcggcctca gcgtcgacga gaacggcgaa
     1621 gtggcgcaat cgctgctgac gcagttcctc acccatgcaa gcctggaagc cggcgaacac
     1681 caagccgaag ccggccggga tgctttacag ttgatgacgg tgcactcggc aaaagggctg
     1741 gagttccaga cggtgttcat cagcggactg gaggaaggtc tgtgccctca tgagcaaagc
     1801 ctctacgaga ccagcggact ggaggaggaa cgccgcctga tgtacgtcgc tgtcacgcgc
     1861 gcgcgccgcc gcctgtatct ttcccacgcg caaagccgca tgctgcatgg tcaggtacgc
     1921 tacggcatcc cgtcacgctt tctggatgag atccccgaaa cgctactcaa acgcctcaac
     1981 gccagcccag ccgcgaagcc atcctataac gcaccggcga caccagccac gaatcagcgt
     2041 accggcatgc cgtggaagat cggccaggcg gtggcgcatg ccaagttcgg acaaggtgtg
     2101 gtggtgaatt acgaaggcaa tgcggacgac ctgcgcgtac aggtcaattt cggccgcgag
     2161 ggcctgaagt ggctggcgct ggaatacgcc aaactacagt cgatctaggc atcggccgcg
     2221 gcttcatgcc agcccggttt gcctgcaacc accacccatc tggcagccac aggtgatgac
     2281 tacagcgacg tgacctcaag cctcgtcggc gtcgagcgca gccgcttccg cctcggaaac
     2341 cggaacgacg aagatatcgc ggtagctggc atagagcgac gcgatcatgg tcgggatcat
     2401 caccagcatg ccgagaaaca gcggcaacgc cgccagtacc agcagtatgg tcgcgatcac
     2461 gctgtagatg aaaaatggca agagattgcg cagacaggcg aggaagcttt gcttcatggc
     2521 ctgcaagccg ctcatctcgt ggaatgccac cagcacaggc gcgaaccagt aagccatcac
     2581 caacggtagc atcaacccta ggccgaccag catggtgagc agaaagccat cattcagggc
     2641 cgctgatgcc tgctccggcg tcagtttctg gccggagacc atggcctgca gcacggcctg
     2701 atcgctgccg gcgaagatga tgccgacgac gacgatcgcg ccagccagat agattccgcc
     2761 cactgtgatc aattgtgccg tgtgatgctt gaagccggcg aacaggtgcg cgatctcaag
     2821 ctcttcgcct ttctccaatg cccggcaacc gatcatgaaa ccggcgacga acaccggggc
     2881 cagcagattg aggacgatag gcccgatgag cttgatggtt gacagcacca tgccgatcaa
     2941 cagatagatg aacagcagcg cgatccagat caccgggctg cggcggaaca tggcgaaccc
     3001 gtcggctagc catttccagc cgtgcagtgc attgagtttt ctggtatcga gattcggact
     3061 gctcatatgg cctccccggg cgtcaggcgt cgagtttcat ggtggttggc tgctggatac
     3121 gcagcttgag gatctgctgg aaatgctgcg gatccttggc gtgggtcaac tcgccgtcct
     3181 gcgggtaatg caggtcgtag agacgcgaca gccagaatcg caaagcagcc atgcgtaata
     3241 agctgggcca tgcctcgcac tccgccgcag tgaatggccg ggcgccgtga taagcccgta
     3301 gcaatgcttg caggcgtggc tgatcgaagg tgccgcccgc tgccacgcac cagtcattgg
     3361 cggcaatggc gacatcgtag agcagcacat cgttacaggc ataatagaaa tcgatgagcc
     3421 cgcctagcgc atcgccatcg aacaagacat tgtcacgaaa aagatcggcg tggatcacgc
     3481 catgcggcag ggcaccgaga tccaggctgg cttcgtaatc cagttccgcc tgcagcatcc
     3541 gctggtccgc ctcgttcagg aaacgcatca cgctgtctgc cgtcgccttg cgccaggctt
     3601 tgtcacgagg attgtccatg tgctggccga acgactgtcc ggccaggtgc aattgcgcga
     3661 gcacccgacc cactgcttca cactgcgcca ggctgggctg cgccacatcc ttgccgcgca
     3721 ggcaggtcac cagtgctgcc ggcttgccgt tcaatgcgcc aaggctgtgc ccgcgctcat
     3781 cggccactgg atgcggacaa ggcaccccgt ggtcggagag atgcgccatg agatcaagga
     3841 aatacggcaa ttcatctgcc gcattgcgct cgaacaacgt cagcacatag cgcccgctgt
     3901 cggtcgtgac gaaataattg gtattggtga tgccggccgc gatgccttta agctcgcgca
     3961 gttcccccag gggataggct ttcagccatt gctgcagttc ttcagtggtg acggtggtga
     4021 atacggacat aaaactttaa gagggctttt cagccctctt caagaaaact agaaattgaa
     4081 cagcacccat ttaggaatga cgagtggcgc ggtcggccct tccatgcggg accagccact
     4141 atcctggtcc tctttcatca ggtagtaagg cacgccatgc ggcggggtga ctttcatcat
     4201 gtagagttcg ccgttgatgc ggtattcctc cacagtgtcc tcacccttct tgatgatggt
     4261 gatggccggt tcagcagcgg ggtcgccgtc gaccacgcct ggcggaggcg gtacgtcggg
     4321 caatggttcc agcttgggat cgctctctgc ggcgaatgcg gccacaggca gggacaaagc
     4381 caacacgatc atggtgcgta acaggcgcat gctaacctct tgcagtaggg tatggcgtga
     4441 ttctaacaaa aagcacccgg ctacagcgta atcccggtaa aaaactccct gtcggataac
     4501 gccgacagcg atagcgccag cggtcgttca agggattggc tagagataga gctggatctg
     4561 gcgctcggcc gccgaaggtt cgaacccgcg ggtctcatag tgcttgaaga tggcctcgac
     4621 caccttggcc ggttcgtcga tcacctgcac caggtccatg tcctcgggcg ataccatgcc
     4681 ctccgtcacc agcgtatcgc ggaaccagtc
//
LOCUS       DATMTW010000247         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_123422, whole genome shotgun sequence.
ACCESSION   DATMTW010000247 DATMTW010000000
VERSION     DATMTW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123422"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1786
                     /locus_tag="VN084_04870"
     CDS             59..1786
                     /locus_tag="VN084_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HWT28821.1"
                     /translation="MDDRTHPITGAEILIRCLQEENVKFVFGYPGGAVLNIYDEIFKQ
                     NHFKHVLVRHEQAAVHAADAYARATGQVGVALVTSGPGATNAVTGIATAYMDSIPMVI
                     ISGQVPTPAIGMDAFQEVDMVGITRPCVKHNFLVKDVRDVAATVKKAFYIAASGRPGP
                     VVVDIPKDVTALTTVFDYPKSVEMRSYTPVTKGHSGQIKKAVKLMLEAKRPMVYTGGG
                     VVLGDASDMLTKLVRDLGFPITNTLMGLGGYPATDKQFLGMLGMHGTYEANMAMQECD
                     VLIAIGARFDDRVIGNPQHFFSKHRTIIHIDVDPSSISKRVKVDVPIVGHVQSVLAEI
                     NELLQAETKRPESEALKAWWDQIETWRSRKCLTYAKSDEVIKPQFVVEKLWEVTKGDA
                     FITSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGAQLAHPEAQVACITG
                     EASIQMCIQELSTCKQFHLPVKVVNLNNRYMGMVRQWQEFFYGNRYSESYMDALPDFV
                     KLAEAYGHVGMKIEKPSDVEAALKEAFSPALKERMVFMDFITDQTENVFPMVPNGKGL
                     SEMILAEEL"
     gene            1825..2316
                     /gene="ilvN"
                     /locus_tag="VN084_04875"
     CDS             1825..2316
                     /gene="ilvN"
                     /locus_tag="VN084_04875"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWT28822.1"
                     /translation="MKHIISLLMENESGALSRVAGLFSARAYNIESLTVAPTEDATLS
                     RMTIVTSGSEDVIEQIIKQLNKLVDVVKVLDLNDGRYIERELMLVKVKATGPFREEMK
                     RMSDIFRGRIIDVADGSYTIELTGSGSKLDAFLEALDKAAILETVRTGASGIGRGDRI
                     LKI"
     gene            2347..3363
                     /gene="ilvC"
                     /locus_tag="VN084_04880"
     CDS             2347..3363
                     /gene="ilvC"
                     /locus_tag="VN084_04880"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWT28823.1"
                     /translation="MKVYYDKDADLSIIKKLKVTIVGYGSQGHAHAANLKDSGVKVTI
                     GARKDGSSYAKAEKAGHDVKDIAKAVKDADVVMILLPDETMAGIFEAEVAPNLKKGAA
                     LAFAHGFNIHYNQIVPRKDLDVIMIAPKGPGHTVRSEYLKGGGVPSLIAVYQDASGKA
                     KDIALSYAAANGGTKGGVIETDFREETETDLFGEQAVLCGGAVELVKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPKVINDESRWAMKE
                     ALANIQNGEYAKKFILEGRTNYPEMTARRRLNAAHPIEQVGGKLRAMMPWISKNKLVD
                     QTKN"
BASE COUNT          815 a         1044 c          961 g          667 t
ORIGIN      
        1 cagactcaaa ccgttagtgt tctgggttct caatttatca cccaaacaag cactgtttat
       61 ggacgaccgc actcacccga tcacgggcgc agaaattctc attcgctgtc tgcaagagga
      121 gaacgtcaag ttcgtcttcg gctacccggg tggtgcagtg ctgaacatct acgacgagat
      181 cttcaagcag aaccatttca agcacgttct cgtccgccat gaacaggcgg ctgtccatgc
      241 ggctgatgct tacgcacgcg ccaccgggca ggtcggcgtc gcgctggtga cctccggccc
      301 cggcgccacg aatgctgtca ctggcatcgc caccgcctac atggattcga tcccgatggt
      361 catcatcagc ggtcaggtac cgacgccagc cattggcatg gacgcgttcc aggaagtcga
      421 catggtcggt atcacccgcc catgcgtcaa acacaacttc ctggtcaagg acgtccgcga
      481 cgtcgctgcg acagtcaaga aagcgtttta catcgcggcc agcggccgcc ccggccccgt
      541 cgtggtcgat atccccaagg atgtcaccgc gctgacgact gtcttcgact atccgaaatc
      601 ggtcgagatg cgttcctaca cgccggtcac caagggtcat tccggccaga tcaagaaggc
      661 tgtcaagctc atgctcgaag ccaagcgccc gatggtctac acgggtggtg gcgtagtact
      721 gggtgacgcc tctgacatgc tgaccaagct tgtccgcgac ctgggcttcc cgatcaccaa
      781 cacgctgatg ggcttgggcg gctatcccgc gaccgacaag cagttccttg gcatgctggg
      841 catgcacggc acctacgaag ccaacatggc gatgcaggaa tgtgacgtgc tgatcgccat
      901 cggcgcacgt ttcgacgacc gcgtcatcgg caatccgcaa cacttcttca gcaagcaccg
      961 gacgatcatc catatcgatg tcgacccttc gtcgatctcc aaacgcgtca aggtcgatgt
     1021 accgatcgtc ggccatgtcc aatcggtctt ggccgagatc aacgagttgc tgcaagccga
     1081 aaccaagcgc ccggaatccg aggcgctcaa ggcctggtgg gaccagatcg aaacctggcg
     1141 cagccgcaag tgcctgacct atgccaagag cgacgaagtc atcaagccgc agttcgtcgt
     1201 cgagaagctg tgggaagtca ccaaaggtga cgccttcatc acctccgacg tcggccagca
     1261 tcagatgtgg gcagcgcagt actacaaatt cgacaagcca cgtcgctgga tcaattccgg
     1321 cggccttggc accatgggcg tcgggctgcc ttacgccatg ggcgcgcaac ttgcgcaccc
     1381 tgaggcacag gtcgcctgta tcacgggtga agcgtcgatc cagatgtgta tccaggagct
     1441 ctccacctgc aagcagttcc acctgccggt gaaagtggtc aacctgaaca accgctatat
     1501 gggcatggtg cgtcagtggc aggagttctt ctacggtaac cgctattcgg aatcgtacat
     1561 ggacgccttg cctgatttcg tcaagctggc ggaagcctac ggccatgtcg gcatgaagat
     1621 cgaaaagcct tccgatgtcg aagcggcgtt gaaggaagcc ttcagccctg ctctcaagga
     1681 gcgcatggtg ttcatggact tcatcactga ccagaccgag aacgtgttcc cgatggtgcc
     1741 gaacggcaag ggcctgtcgg agatgattct ggccgaagaa ctctaagcag aacgattaag
     1801 cagaacaata aagacggacc cactatgaaa cacatcatct cgctgctgat ggaaaatgag
     1861 tccggcgcgc tctcacgcgt cgccggcctg ttctcggcac gtgcctacaa tatcgaatcc
     1921 ctgaccgtgg cgccgacgga agacgccacg ctgtcgcgca tgaccatcgt cacctccgga
     1981 tcggaagacg tgatcgagca gatcatcaag cagctcaaca agctggtcga tgtggtcaag
     2041 gtgctggacc tgaatgacgg tcgctacatc gaacgtgaac tgatgctggt caaggtcaag
     2101 gccacagggc cgttccgcga ggaaatgaag cgcatgagcg atatcttccg cggccgcatc
     2161 atcgatgtcg ccgacggcag ctacaccatc gagctcactg gctcaggctc caagcttgac
     2221 gcgttcctcg aggcactcga taaagccgcg atcctcgaaa ccgtccgtac cggcgcttcg
     2281 ggcatcggtc gtggcgaccg catcctcaag atttaatctc taaagatttg tactgaaagg
     2341 tagaacatga aagtttatta cgacaaagat gcggacctct ccatcatcaa gaaactcaag
     2401 gtcaccatcg ttggttacgg ctcacagggt cacgcccacg ccgccaacct gaaggattcc
     2461 ggtgtcaagg tcaccattgg cgcacgcaag gacggcagct cctatgccaa ggccgagaaa
     2521 gccggccatg acgttaagga tatcgccaag gcagtcaagg acgccgatgt ggtcatgatc
     2581 ctcttgccgg acgagaccat ggcaggcatc ttcgaagcgg aagtcgcgcc taacctgaag
     2641 aaaggcgcag cactggcctt cgcacacggc ttcaacatcc attacaacca gatcgtcccg
     2701 cgcaaggatc tggacgtcat catgatcgcg ccaaaaggcc caggtcacac cgtgcgttcc
     2761 gaatacctga agggtggcgg cgtgccttcc ctgatcgcgg tctaccagga tgcctccggc
     2821 aaggccaagg acatcgctct ttcctacgct gcagcaaacg gcggcaccaa gggtggcgtc
     2881 atcgagaccg atttccgtga agagactgaa accgacttgt tcggtgagca ggccgtgctt
     2941 tgtggtggcg cagtcgaact ggtcaaggct ggtttcgaaa ccctagtgga agctggctat
     3001 gcacctgaaa tggcttactt cgaatgtttg catgaactga agctgatcgt cgacctcatg
     3061 tacgaaggcg gtatcgccaa catgaactac tcgatctcca acaatgctga gtacggcgaa
     3121 tacgtcaccg gccccaaggt catcaatgac gagtcccgct gggccatgaa ggaagcgctg
     3181 gccaacatcc agaacggcga atacgccaag aagttcatcc tggaaggccg cacgaactat
     3241 cctgagatga cggcacgtcg tcgcttgaac gccgcacatc cgatcgagca ggtcggcggc
     3301 aagctgcgcg ccatgatgcc atggatcagc aagaacaaac tggtcgacca aaccaagaac
     3361 taagcgtcgt caccattaag tcagccagaa agggcaggcc gcgctagcgc cctgcccttt
     3421 ctgcttttag ccgctataat ccacgaaaac tgttcaggta aaccgcgtgt cagatcgcct
     3481 attcgtc
//
LOCUS       DATMTW010000248         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_592736, whole genome shotgun sequence.
ACCESSION   DATMTW010000248 DATMTW010000000
VERSION     DATMTW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592736"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /gene="leuC"
                     /locus_tag="VN084_04885"
     CDS             complement(<1..1162)
                     /gene="leuC"
                     /locus_tag="VN084_04885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWT28824.1"
                     /translation="MAKTLYDKLFDSHVVHEENGAALLYIDRHLVHEVTSPQAFEGLK
                     MSARKVWRVNSVLAVPDHNVPTTDRSKGIADPVSRLQVETLDNNCAEFGITEFRMNDT
                     RQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVQK
                     KSKAMLVKVEGQLGRGVTAKDIALAVIGRIGTAGGTGYAIEFAGSAIRGLSMEGRMTL
                     CNMAIEAGARAGMVAVDDTTIDYVKGRPYAPKAEQWEAAVAYWRTLHSDEGAPFDAII
                     ELDAAAIQPQVTWGTSPEMVTSINGAVPDPAKEADPTKRGGMERALEYMGLQANTPID
                     QISVDKVFIGSCTNSRIEDLRAAAAIAKGKRVASNVKLAMVVPGSGLVKAQAE"
     gene            complement(1177..1671)
                     /locus_tag="VN084_04890"
     CDS             complement(1177..1671)
                     /locus_tag="VN084_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HWT28825.1"
                     /translation="MPSFDITSEADMVALKNAVDVADRQITNRYDFKGTSAKVDLNEK
                     DKLITLFGDSDFQLDQIKDILFPAMEKKEPDSSKRLDLQDVQKVSGNKAKQEMKIKDG
                     IDSDLAKRLVKLIKDSGLKVQASIQGDTVRVNGAKRDVLQETIAHVKKSVTDFPLKFG
                     NFRD"
     gene            complement(1727..2038)
                     /locus_tag="VN084_04895"
     CDS             complement(1727..2038)
                     /locus_tag="VN084_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HWT28826.1"
                     /translation="MAQFDNVSVKKKANVYFDGKCVSHTVMFPNGTRCTVGVIFPSTL
                     TFNTGSPELMEINAGVCKVRLKGDENWKSYGAGEKFAVPGNSSFDIETVETLDYVCHF
                     E"
     gene            complement(2060..>2967)
                     /locus_tag="VN084_04900"
     CDS             complement(2060..>2967)
                     /locus_tag="VN084_04900"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWT28827.1"
                     /translation="ANETGADAISHGATGKGNDQVRFELGAYALKPGVKIIAPWREWD
                     LLSREKLLAYAEKNGIPVEMKHKQGGSPYSMDANLLHISYEGRHLEDPSAEAEESMWR
                     WTVSPENAPDAAEYLDIEYVHGDPVALNGKKMAAHELLAELNKLGGKHGIGRLDLVEN
                     RYVGMKSRGCYETPGGTIMLKAHRAIESLTLDREVAHLKDDLMPRYASLIYNGYWWSP
                     ERVALQTLIDHTQKSVNGYVRLKLYKGNVIVTGRDSKTDSLFDPTIATFEDDAGAYDQ
                     KDAGGFIKLNALRMRIAANLRSKKK"
BASE COUNT          530 a          918 c          833 g          686 t
ORIGIN      
        1 gctcggcctg ggccttcacc agaccggaac caggcaccac catggccagc ttgacgttgg
       61 acgcgacacg cttacctttg gcgatcgccg ccgcagctcg cagatcctcg atgcgcgagt
      121 tggtacaaga gccaatgaac accttgtcga cactgatctg gtcgattggg gtattggcct
      181 gcaaacccat gtactcgagt gcacgctcca tgccaccgcg cttggtcgga tcggcctctt
      241 tcgcaggatc cggcaccgca ccattgatgc tggtcaccat ctcgggggat gtcccccagg
      301 tcacctgcgg ctggatggca gcggcatcga gttcaatgat ggcgtcgaac ggcgcgcctt
      361 cgtcgctgtg caacgtgcgc cagtaggcga ctgccgcctc ccattgctcg gccttgggcg
      421 cgtaaggacg ccccttgaca tagtcgatgg tggtgtcatc cacggccacc atgccggcgc
      481 gggcgccagc ttcgatcgcc atgttgcata gcgtcatgcg gccttccatg gaaaggccgc
      541 ggatcgccga accggcgaat tcgatggcat agccggtacc gcccgcggtc ccgatcctgc
      601 cgatcaccgc cagcgcgata tccttggcgg tgacgccacg cccgagctgc ccttccacct
      661 tgaccagcat ggccttggac tttttctgca ccaggcattg cgtcgccagg acgtgctcca
      721 cttccgaggt gccgatgcca tgagccagcg caccaaaggc gccatgcgta ctggtatgcg
      781 aatcgccgca gactaccgtc atgcccggga gcgtcgcgcc ttgttctggc ccgatgacgt
      841 gcacgatgcc ctgtcgcgtg tcgttcatgc ggaattcggt gataccgaac tcggcacagt
      901 tgttatccag cgtttccacc tgcaggcgcg acaccggatc tgcgatgccc ttgctgcgat
      961 cggtggtcgg cacgttgtgg tcaggcacgg ccagcacgga attgacgcgc cataccttgc
     1021 gcgcgctcat cttcaggccc tcaaaggcct gcgggctggt gacttcgtgc accagatgac
     1081 ggtcgatata gagcagcgcg gcgccgttct cttcgtgcac cacgtgggaa tcgaataatt
     1141 tgtcgtatag cgtctttgcc acggttgttc ctgcgattag tctctgaaat taccgaactt
     1201 caacgggaag tccgtcaccg atttcttcac atgggcgatg gtctcttgca aaacgtcgcg
     1261 cttggcgccg ttgacgcgca cagtatcgcc ctggatgctc gcctgcacct tgagtccgga
     1321 atccttgatg agcttgacca agcgtttcgc cagatcgcta tcgatgccgt ccttgatctt
     1381 catctcctgc ttggccttgt tgccggatac cttctgcaca tcctgcaggt ccagacgctt
     1441 gctgctatcc ggctccttct tctccatcgc cgggaacagg atgtccttga tctggtcgag
     1501 ctgaaaatcg gaatcgccga acaacgtgat cagcttgtcc ttctcgttca gatccacctt
     1561 ggcggacgtg cccttgaaat catagcggtt ggtgatctgg cgatccgcca catcgaccgc
     1621 attcttcagc gcaaccatgt cggcttctga cgtgatatca aacgatggca ttgtcttctc
     1681 ctaatagtct cttaactgct gcttcagtgc ctcaacggcg ggcagatcac tcgaagtggc
     1741 agacgtagtc cagggtctcg acggtctcga tatcaaagct tgaattgcca ggcactgcga
     1801 acttctcgcc ggcgccatag cttttccagt tctcatcgcc cttgaggcgc accttgcaga
     1861 cgccggcgtt gatctccatg agctcaggcg aacccgtatt gaaggtcagc gtgctcggga
     1921 agatcacacc cacggtacag cgcgtaccgt tcggaaacat cacggtgtgg ctgacgcatt
     1981 tgccatcgaa atagacgttg gctttctttt tgacgctgac attgtcgaac tgtgccatct
     2041 tctctctcca aatctttcgt tattttttct tgctgcgtaa gttggcggca atccgcatgc
     2101 gcaaggcatt caacttgatg aagccgcctg cgtccttctg gtcgtaggcc ccggcatcgt
     2161 cttcaaaagt cgcgatggtc ggatcgaaca gcgaatcggt cttgctgtcg cgaccggtca
     2221 cgatgacatt gcccttatag agcttgaggc gcacgtaacc gttgacgctt ttctgcgtgt
     2281 ggtcgatcag cgtctgtaac gcgacgcgct ccggactcca ccagtagccg ttgtagatga
     2341 ggctggcata gcgcggcatc aagtcatcct tgagatgcgc gacttcacgg tccagcgtca
     2401 acgactcgat cgcgcgatgc gccttgagca tgatggtgcc accgggcgtc tcgtagcagc
     2461 cgcgtgattt catgccgaca taacgattct cgaccagatc gagacgaccg atgccatgct
     2521 tgccgcccag cttgttgagt tcggcgagca gctcatgcgc cgccatcttc ttgccgttca
     2581 gggcgacggg gtcgccatgc acgtattcga tgtcgagata ttccgcggca tccggcgcgt
     2641 tctcgggtga cacggtccag cgccacatgg attcctctgc ttcagcagac ggatcttcca
     2701 ggtggcggcc ttcatagctg atatgcagca ggttggcgtc catggaatac gggctgccgc
     2761 cttgcttgtg cttcatctcg accgggatgc cattcttttc ggcataggcc agcagcttct
     2821 cacggctcag cagatcccat tcacgccacg gcgcgatgat cttgacgccg ggcttgagcg
     2881 cataggcgcc cagttcgaaa cgcacctggt cgttgccctt gccggtggca ccatgcgaga
     2941 tagcgtcggc gccggtctca ttggcga
//
LOCUS       DATMTW010000249         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_323309, whole genome shotgun sequence.
ACCESSION   DATMTW010000249 DATMTW010000000
VERSION     DATMTW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323309"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VN084_04905"
     CDS             <1..621
                     /locus_tag="VN084_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HWT28828.1"
                     /translation="TASMSLLEAAQAAGADLILVHHGYFWRNEPPEVIGIKRNRLKFL
                     LQHDLNLAAYHLPLDAHPEFGNNVQLGQVMGFDVQRWLGDPAIVACAELQQPMALGDL
                     GQRIATRLQRSPLVIGDAKGVVKRIAWCTGAAQSYMETAIHAGVDVFVSGEISEQTVH
                     LARESGVAYIAAGHHATERYGVQALGQHLADRFGLDHSFVDIDNPV"
     gene            724..1320
                     /gene="petA"
                     /locus_tag="VN084_04910"
     CDS             724..1320
                     /gene="petA"
                     /locus_tag="VN084_04910"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWT28829.1"
                     /translation="MANQPVDHGKRRFLVAATTVVGGTAAAAVAVPFVSSMLPSERAK
                     AAGAPVQIDISKVESGAMITAEWRGKPVWIINRSPEMLANLANHDDRLTDPKCAVTTQ
                     QPAYCNNPSRSIKPNLMVLVGICTHLGCSPSPKLQASGDMGADWPGGFLCPCHGSKFD
                     LAGRVFNGSPAPINLVVPPHKYLSDTLLLVGEDEKGAA"
     gene            1322..2617
                     /locus_tag="VN084_04915"
     CDS             1322..2617
                     /locus_tag="VN084_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HWT28830.1"
                     /translation="MANNLQKYAAGALGWIDERFPLTANWKAHLSEYYAPKNFNFWYF
                     FGSLALLVLVLQIITGIFLTMHYKPDAEMAFGSVEYIMRDVPWGWLIRYMHSTGASMF
                     FVVVYLHMFRGLIYGSYRNPRELIWLFGLAIFLALMGEAFFGYLLPWGQMSYWGAQVI
                     VNLFATIPFIGPDVAEWLRGDFLVSDATLNRFFAFHVIALPLVLLGLVAAHIVALHEV
                     GSNNPDGVEIKKHKDPKTGIPLDGIPFHPYYTVKDIVGVSVFLIVFSAIVFFAPEMGG
                     YFLEANNFVPADPFKTPPHIAPVWYFTPYYSILRAVPPLFNSQFPGVAAMGLSVVIFA
                     ALPWLDRSPVKSIRYRGNVYKTWLAVFVISFIVLGYLGTVPSTVWGTFDTAIFLVGGA
                     DRATVVARIATVAYFLFFILMPWYTRIDKTKPVPERVTG"
     gene            2617..3345
                     /locus_tag="VN084_04920"
     CDS             2617..3345
                     /locus_tag="VN084_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HWT28831.1"
                     /translation="MKKFLLLLVFLPVMAFASEETVHLDHAPIDPDNQASLQRGARTF
                     VNYCLSCHSAAYMRYNRLRDIGLSEAQIKDNLLFAGEKVGETMKVAMPRVNAKAWFGA
                     APPDLSVEARARTADWLYSYLRGFYRDDTRPTGWNNVVFDKVAMPHVLWQLQGQQILK
                     TEELTDADGHVTHMHKLVLDKPGLMSTAEYDQTIADLVNYLVYMSEPGKPLRMELGLV
                     VLLFLSLLFVVAFYLKKEFWKDIH"
     gene            3421..4020
                     /locus_tag="VN084_04925"
     CDS             3421..4020
                     /locus_tag="VN084_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWT28832.1"
                     /translation="MMTLYSGTTDPYSHRCRIVLFEKGMDFQVIDVDLANKPEDLAVI
                     NPHNEVPVLVERDLVLQEANIINEYIDERFPHPQLMPADPVMRARARLFLHNFEQQLF
                     AHIKDIESGNQKAADKARATIRDNLTQIVPLFSKQQYILGDEFSMLDVAIAPLLWRLG
                     HYGIELPKQSAPLLKYAERIFSRPAYIEAMTPSEKAMRK"
     gene            4023..>4395
                     /locus_tag="VN084_04930"
     CDS             4023..>4395
                     /locus_tag="VN084_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HWT28833.1"
                     /translation="MSEPASSKPYLVRALHEWCMDNALTPHLLVKVDGRTRVPLAYVK
                     DGEIVLNLSYSATKGLNIENEAISFSARFGGVSQNLYIPMSAVRGIFARENGQGMFFE
                     VEAESTESVEGVSDAERGGDDE"
BASE COUNT          864 a         1262 c         1274 g          995 t
ORIGIN      
        1 accgcaagca tgtcgctact tgaggccgcc caggcagcgg gcgccgatct catcctcgtg
       61 catcatggct acttctggcg caatgagccg cctgaggtga tcggcatcaa gcgcaatcgc
      121 ttgaaattcc tgttgcagca cgatctgaat cttgcggctt atcacctccc gctggacgcc
      181 catcccgagt tcggtaacaa cgtacagctc gggcaggtga tgggatttga cgttcagcga
      241 tggctgggcg accctgccat cgttgcctgt gcggagctgc agcagcccat ggcgctgggg
      301 gatctgggtc aacgtatcgc cactcgcctg caacgctcac cgctggtgat cggagacgcg
      361 aaaggtgtgg tcaagcggat cgcctggtgc acgggcgctg cgcagtcgta catggagacc
      421 gcaatccacg ccggggtcga cgtcttcgtc agcggcgaga tctcggaaca gaccgtgcat
      481 ctggcgcgag agtccggcgt cgcctacatt gcggccgggc atcatgcgac tgaacgctac
      541 ggcgtgcagg cgttaggaca acacctcgca gaccggttcg gtctcgatca cagcttcgtc
      601 gatatcgata accccgtata aataccgttc tgggccaaac acctgtttaa gttttgcggc
      661 ggaaaaggta aaatgccggg tttgaatcaa tcaacatcaa tcgcaccaga ttaagggaat
      721 gtgatggcaa atcaaccagt ggaccatggt aagcgacgtt tcctcgttgc cgccaccacg
      781 gtcgtaggcg gcactgccgc cgccgcagta gcggtacctt tcgtatccag catgttgccg
      841 agcgagcgag ccaaagcggc cggcgcgcca gtccaaatcg atatcagcaa ggtggaatcg
      901 ggcgccatga tcacggcaga gtggcgcggc aagccggtat ggatcatcaa ccgctcaccg
      961 gagatgctgg cgaacctcgc caatcacgac gacaggctga ccgaccccaa gtgtgcggtg
     1021 accactcagc aacctgccta ctgtaacaac ccatcgcgtt ccataaagcc taacctcatg
     1081 gtgctggtcg gcatctgcac ccacctgggg tgttcgcctt cgccaaagct gcaggccagt
     1141 ggcgatatgg gtgctgactg gccgggcggc ttcctgtgcc cttgccatgg ctccaagttc
     1201 gacctggctg gtcgcgtgtt caatggctcg ccggccccga tcaacctggt agtgccgccg
     1261 cacaagtatc tgagcgatac cttgctgctg gtgggcgaag atgagaaagg tgctgcataa
     1321 catggcgaac aatctgcaaa aatatgccgc tggcgcactg ggctggatcg atgagcgctt
     1381 cccgcttacc gccaactgga aagcgcatct ctcagaatac tacgcgccca agaacttcaa
     1441 tttctggtat ttcttcggct ctctggcgct gctggtgctg gtgctacaga tcatcacggg
     1501 tatcttcctg accatgcatt acaagcccga tgccgaaatg gcgttcgggt ctgtcgaata
     1561 catcatgcgg gatgtaccct ggggctggct gatccgttac atgcattcca ccggtgcgtc
     1621 catgttcttc gtcgtggtct acctgcacat gttccgcggt ctgatctacg gctcctatcg
     1681 taatccacgt gagctgatct ggctgttcgg cctggccatc ttcctggcat tgatgggcga
     1741 ggccttcttc ggctatctgc tgccatgggg gcagatgtcc tactggggtg cgcaggtgat
     1801 cgtcaaccta ttcgcgacga tcccgttcat cggtccggat gtcgcggagt ggctgcgtgg
     1861 tgacttcctg gtatcggatg cgacgcttaa ccgctttttc gcgttccatg tgattgcctt
     1921 gccgctggtg ctgctgggtc tggtggcggc tcacatcgtc gctttgcatg aggtgggttc
     1981 caacaacccg gatggcgtcg agatcaagaa acacaaggat cccaagaccg gcattccgct
     2041 cgatggcatt ccattccacc cctactacac ggtcaaggat atcgtcggtg tgtccgtctt
     2101 cctgatcgtc ttctccgcga tcgtgttctt cgcgccggag atgggtggct atttcctgga
     2161 ggccaacaac ttcgtcccgg ctgatccgtt caagacgccg ccacatatcg cgccggtctg
     2221 gtatttcacc ccttactatt ccatcttgcg tgccgtgcct ccgctcttca attcccagtt
     2281 ccctggtgtt gcggcgatgg gtctgtcggt ggtgattttt gccgccttgc catggctgga
     2341 tcgtagtcct gtgaaatcca tccgatatcg cggtaatgtc tacaagacgt ggttggccgt
     2401 attcgtcatc agcttcatcg tcctcgggta cctcggtacc gtaccttcga cggtttgggg
     2461 cacctttgac accgcgatct tcctcgtcgg cggcgctgac cgtgcgactg tggtggcgcg
     2521 gatcgctacc gtcgcgtatt tcctgttctt catcctgatg ccttggtata cgcggatcga
     2581 taaaaccaaa cctgtgccag aaagggtgac cggataatga aaaagttctt gttgttgctg
     2641 gtttttctgc cggtgatggc gtttgcctcc gaagagaccg tccatctgga tcatgcgcct
     2701 atcgatccgg acaaccaggc atccttgcag cgtggtgcca ggaccttcgt caactattgc
     2761 ctgagctgtc acagcgcggc ttacatgcgc tacaaccgcc tgcgcgatat cggcttgtcc
     2821 gaggcgcaga tcaaggacaa tctgctgttc gccggcgaaa aggtgggcga gaccatgaag
     2881 gtggccatgc cgcgcgtcaa tgccaaggcc tggttcggtg cggcaccgcc cgatctgtcg
     2941 gtcgaggcgc gcgcgcgaac ggccgactgg ctatatagtt acctgcgcgg cttctaccgc
     3001 gatgacacgc ggccgaccgg ctggaacaac gtggtcttcg acaaggtggc catgccgcat
     3061 gtcctgtggc aactgcaggg ccagcagatc ctcaagaccg aagagctgac ggatgccgac
     3121 ggacacgtaa cgcacatgca caagctggtg ctggacaagc caggcctgat gtcgacggca
     3181 gaatacgatc agaccatcgc cgatctggtg aattatctgg tctatatgtc tgagcccggc
     3241 aagccattgc gcatggagct gggtctggtc gtgctgctgt tcctgagtct gctctttgtc
     3301 gttgcgttct acctcaagaa agagttctgg aaagacatac attaatcgaa gtgctacggg
     3361 agttgccgcg ttcgcggcac gctccctttt ttgctttata gcgagtagga aaccaaaatc
     3421 atgatgacct tatattcagg cactaccgac ccttacagcc atcgttgccg tatcgtcctg
     3481 ttcgagaagg gcatggattt ccaggtgatc gatgtcgacc tggccaacaa gcccgaggat
     3541 ctggctgtca tcaatccgca taatgaggtg cctgtgctgg tcgagcgcga cctggtgttg
     3601 caggaagcca atatcatcaa cgaatacatc gatgagcgct tcccgcatcc gcaactgatg
     3661 cctgctgatc ctgtgatgcg tgcccgtgcg cgcctgttcc tgcataactt tgagcagcag
     3721 ctgttcgcgc acatcaagga catcgaatcc ggcaaccaga aggcggctga caaggcgcgc
     3781 gccaccatcc gcgacaacct gacgcagatc gtgcccctgt tcagcaaaca acaatatatt
     3841 ctgggcgatg agttttccat gctcgacgtg gcgattgcgc ccctgctctg gcgcctcggc
     3901 cattacggca tcgagttgcc taagcagtcc gcgcctttac ttaaatatgc ggagcgtata
     3961 ttctctcgcc cggcttatat cgaggcgatg acgccgtccg aaaaggcgat gcgcaaatag
     4021 acatgagcga gccagcttcc agcaagccat acctggtgcg tgcattgcac gagtggtgta
     4081 tggataacgc gctcacgccc cacttgctgg ttaaggtgga tggccggaca cgcgtgccgc
     4141 tggcttatgt gaaagatggc gagatcgttc tcaatctgag ctacagcgcg accaagggcc
     4201 tgaatatcga gaacgaggcg atctcgttct cggcaagatt cggtggggtc tcgcagaact
     4261 tatatatacc gatgtcggct gtgcgtggca tttttgcccg cgagaatggc cagggcatgt
     4321 tcttcgaggt cgaggcggag tcaacggaat cggtagaggg cgtcagtgat gccgagcggg
     4381 gcggtgatga tgagc
//
LOCUS       DATMTW010000250         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_660014, whole genome shotgun sequence.
ACCESSION   DATMTW010000250 DATMTW010000000
VERSION     DATMTW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660014"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..638
                     /locus_tag="VN084_04935"
     CDS             171..638
                     /locus_tag="VN084_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HWT28834.1"
                     /translation="MQDQLDRISSHVESHQAMPGGLLPLLHAIQDDLGYIPQESYAPI
                     SKALNLSVAEIHGVVSFYHHFRTQPPGRHVLHLCRAESCQAMGCQSLETHIKQQLGID
                     FHETSSDGAITLEPIYCLGNCALSPAVMLDERVHGRMNIAEIDALLAAAREVA"
     gene            635..2188
                     /locus_tag="VN084_04940"
     CDS             635..2188
                     /locus_tag="VN084_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWT28835.1"
                     /translation="MSIRVYVPCDSGALSVGAEKVADLIQVEAARRRADVKIVRNGSR
                     GLYWLETLVEVETPAGRVAYGPVRPEDVPGLFDSDFLHGGRHALHHGLTDDMPWLKQQ
                     ERLTFARVGITDPLSVEDYVAHAGFRGLRNALAMTPADIVQQVTDSGLRGRGGAAFPT
                     GIKWKTVLGCEGEQKYIVCNADEGDSGTYSDRMIMEGDPFVLIEGMTIAGLAVNADKG
                     YIYLRSEYPDALVMLNRAIAIARAQGYLGEDILGSGRNFDLEVRRAAGAYICGEETSL
                     LESLEGKRGLVRFKPPLPAIEGLFGKPTVVNNVISFASVPIILDQGAEYYRDYGMGRS
                     RGTLPIQLAGNIKHCGLVEKAFGVTLRELLYDFGGGSASGRPIRAVQVGGPLGAYLPE
                     SQFDTPLDYEKFSEIWAVLGHGGIVAFDDTVDMAKMARYAFEFCAIESCGKCTPCRIG
                     STRGVEVMDKIMAGNDRTRNIKLVRDLSDTLLNGSLCALGGMTPYPVLSALNHFPEDF
                     GLQQGDIAA"
     gene            2219..>2788
                     /locus_tag="VN084_04945"
     CDS             2219..>2788
                     /locus_tag="VN084_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWT28836.1"
                     /translation="MDTQLKQLDYGTPAKDNTLEVTLEIDGREVTVPAGTSVMRAAME
                     AGISVPKLCATDSLEPFGSCRLCLVEIDGRRGYPASCTTPVDAGMKVHTQTPKLADIR
                     RGVMELYISDHPLDCLTCAANGDCELQDMAGAVGLREVRYGYEGENHLKAEKDTSNPY
                     FTFDPSKCIVCSRCVRACEETQGTFALTIE"
BASE COUNT          562 a          821 c          835 g          570 t
ORIGIN      
        1 atgtctaacg tcagtggatt atagtcaagt gcgattattt ttatctagcg catgcgcatt
       61 atttgacagt ggccgatttt aagacacaga atacagcgga tgtcatgcgt gatctgaatg
      121 atcccaacag aataacgata aatgcaggct aagttcgagg agtggagtcg atgcaagacc
      181 agctggaccg tattagttct catgtcgagt ctcatcaggc gatgccagga gggctgctgc
      241 ccttgctgca tgccattcag gatgacttgg ggtatatccc gcaggagagt tatgccccga
      301 tctccaaggc gctcaacctg tcggtcgccg agatccatgg cgtcgtcagc ttctatcacc
      361 atttccgcac gcagccaccg ggccgccatg tcttgcatct atgccgcgcc gaatcctgtc
      421 aggccatggg atgccaatcg ctcgaaacgc acatcaagca acagcttggg attgattttc
      481 acgagaccag cagcgatggc gcgatcaccc tcgaacccat ctactgtctc ggcaactgcg
      541 cgctctcgcc cgccgtgatg ctcgatgaac gcgtgcatgg ccgcatgaat atcgccgaga
      601 tcgatgcctt gctggcggca gccagggagg tcgcatgagc atccgtgtct atgttccttg
      661 cgattccggg gcgctgtctg tgggggcgga aaaggtcgct gacctgatcc aggtcgaggc
      721 cgcacgccgg cgcgccgacg taaagatcgt gcgcaacggt tcgcgtgggt tgtactggct
      781 ggaaacgctg gtcgaggtgg agacgccggc gggacgcgtc gcttatggcc cagtgcggcc
      841 cgaggatgtt ccgggcctgt tcgacagtga cttcctgcat ggcggccgcc atgccttgca
      901 tcacgggctc accgatgaca tgccctggct aaagcagcaa gaacgcctga cctttgcccg
      961 cgtcggcatc accgatccgt tgtcagtcga ggattacgtc gcccatgccg gtttcagggg
     1021 cttacgcaac gcactggcca tgacgcctgc cgatatcgtg cagcaagtga ccgattccgg
     1081 tttgcgcggc cgcggtggtg cagctttccc aacgggtatc aagtggaaga ccgtgctggg
     1141 ttgcgagggt gagcagaaat acatcgtctg caacgccgat gaaggcgatt ccggcaccta
     1201 ttccgaccgc atgatcatgg aaggcgatcc tttcgtgctg atcgagggca tgaccatcgc
     1261 cggtctcgcc gtcaatgccg acaagggcta catctacctg cgttcggaat acccggatgc
     1321 gctggtcatg ctcaaccgtg cgatcgccat cgcccgtgca caaggctatc tcggcgagga
     1381 tatcctcggc agcggccgca acttcgatct cgaagtgcgt cgcgccgctg gcgcttacat
     1441 ctgcggcgaa gagacttcac tgctggagag cctcgaaggc aaacgcggcc tagtacgttt
     1501 caagccgcca ttgccagcga tcgaaggctt gttcggcaag ccgaccgtcg tcaacaacgt
     1561 catctctttc gcaagtgtcc ccatcattct cgaccagggc gccgagtatt accgcgatta
     1621 cggcatgggc cgttcgcgtg gcacgctgcc gatccagctg gctgggaaca tcaagcattg
     1681 cggattggtc gaaaaagcat tcggggtcac cttgcgtgag ctgctctacg atttcggcgg
     1741 cggatccgca tccgggcggc cgatccgcgc ggttcaggtc ggcgggccac tgggcgccta
     1801 cctgcctgaa tcacagttcg acaccccgct cgattacgag aagttcagcg agatttgggc
     1861 ggtgcttgga cacggcggta tcgtggcctt tgacgatacg gtggacatgg cgaagatggc
     1921 acgctacgcc ttcgagttct gcgcgatcga atcctgcggc aaatgtacgc catgccgcat
     1981 cggctcgaca cgtggtgtgg aagtgatgga caagatcatg gccggcaacg atcgtacccg
     2041 caacatcaag ctggtgcgcg atctgagcga taccctgctc aatggctcgc tatgtgcgct
     2101 gggcggcatg acgccgtatc ccgttctcag cgcgctcaat cacttcccgg aagattttgg
     2161 cctacagcag ggcgacatcg ccgcctgagg ccagcactca ttagtctgga gcaagaagat
     2221 ggatacgcaa ctcaagcaac tggattacgg aacaccggca aaagacaata cgctcgaagt
     2281 cacgctggag atcgatggcc gcgaggtcac cgtccctgcc ggtacatcgg tgatgcgcgc
     2341 cgccatggaa gcggggatca gcgttcccaa gctctgtgcg accgacagcc tggaaccctt
     2401 cggctcctgt cgattgtgcc tggtcgagat cgacggcagg cgcggttatc ctgcctcttg
     2461 cacgaccccg gtcgatgctg gcatgaaggt ccacacgcag acgcccaagc tcgctgacat
     2521 ccgccgtggc gtgatggagc tgtatatctc cgaccatccg ctggattgcc tgacctgtgc
     2581 cgccaacggc gactgcgagc tgcaggatat ggccggggcc gtgggcttgc gtgaggtacg
     2641 ttacggctat gaaggcgaga accatctcaa ggccgagaag gacacgagca acccgtattt
     2701 caccttcgac ccttcgaaat gtatcgtctg ttcgcgctgc gtacgcgcct gtgaagagac
     2761 gcaaggcacc tttgcactga ccatcgaa
//
LOCUS       DATMTW010000251         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_21828, whole genome shotgun sequence.
ACCESSION   DATMTW010000251 DATMTW010000000
VERSION     DATMTW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21828"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VN084_04950"
     CDS             <1..1031
                     /locus_tag="VN084_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWT28837.1"
                     /translation="HTTIDNKDGLLPSSDPATLTAEHSYNRINPAIGLNFTPSSALTV
                     YGTYNEASRAPTSIELGCANPAQPCKLPNAFAGDPPLDQVVSKTYEGGLRGQFDNGVR
                     WSTAAYVTNNYDDILFAAAPGVAGQGYFKNFGQTRRKGIEASLLGNMERFSWSVGYSL
                     VDARYRSSESIASEVNSAAIDTDGDGEGDTLRIRSGDRIPGIPMHQLKMRGVFTVTPK
                     WTIGTSIIAFTDQYARGNENNAHRPDNVDFFGDGKVSGYAVVNLDTRYHLGNGWQVFA
                     KAVNIFDREYNNAGLLGASLFNANGSFNGDEDAGRQALYAPGAPRAGWIGVRWEFGGA
                     KSSGIDTN"
     gene            1328..3700
                     /locus_tag="VN084_04955"
     CDS             1328..3700
                     /locus_tag="VN084_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWT28838.1"
                     /translation="MKRVVPFRLRAVVGLVAGLATLSVMAEEVSEPDAGKPARAEKIE
                     VISTTPLPGIGAPIEEIPANVQSASSKNIEPQHTQDIADYINTNLGSVTINEGQSNPY
                     MSDVFFRGFTASPLPGAPQGLSVFQDGVRINEPFGDTVNWGLIPHTAIASINLMPGSN
                     PIFGLNTLGGALSINTKSGSQYPGLVMEASGGSWGRKGFNASYGGKQGDIDYFVTGNF
                     SSEDGWREHSPSQVNQLFGKFGWQNDSSTLDLTLSAAHNNLEGVQALPRSWLNTPKQA
                     YTYPDSVTYRMQMAALKGTHFIDENNLLAGNIYVRKSRIGNFASNTNDCFGSTDTAAG
                     EECDQTLPDPTDLATQGFNERSSTDQLGWGFTGQYSYLADLFKHRNQLTTGFSFDHGT
                     TRYKSAEQDTAFTGRAATPLSDFAEETNIRGENSYYGLFATDTFSVTDKFHLTMSGRY
                     NYVRVKLKDYMGPDDNTGKYVTGDSPGIVHTFNRLNPAVGFNYNPNDAIGYYGAYNEG
                     MRAPTPIELACADPLTPCRLPTDFLADPSLKPVVSKTWEAGLRGLFNSDWRWNASVFR
                     SRLTDDIQFISSEATNFGYFKNVGETRRQGLELGLTGKVERLRVAANYQYLQATFQSA
                     VTFRNENNSSADANGNYRTRPGDNIPNIPRHSLKLRLAYDVTRALTVGVNTITASSIY
                     ARGDENNKDDNGKIGGYTVVNLDANWRLNPNWTAFTRAVNIFDRDYQTLGVLGVNAFN
                     TADRSFNTGGTDSWVPEQFRTPAPPRAAWFGLRYEFGTNPQSASGSADPD"
     gene            3764..5191
                     /locus_tag="VN084_04960"
     CDS             3764..5191
                     /locus_tag="VN084_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWT28839.1"
                     /translation="MNARRILVVEDETLFARAVCKRLQKAGYECEHAENLHDGRAIAR
                     QFVPDIALLDMRLPDGNGLDLLTELVAKGVTVIVMTAYGEVADAVSAVKQGAIDYLKK
                     PVDLEELLLVIQKAEQSTTLKHHLDYSRQRDAHAAEGIELLGQSPAMQSVRSQVERIS
                     QLVTIETAAPPTVLINGETGTGKDVVARLLHVSCANSDRPFVHVDCASLPADLIESEL
                     FGHEKGAFTSAQGARPGLIEAAEDGTLFLDELGELPLALQAKLLNVLERRVVRRLGST
                     RERAVPARFVAATNRDLHQMVLDGRFRSDLYYRLNVLTILMPPLRERGGDVLLLARHF
                     AAQTERRYGLPQRDFAADAIEMMGNYPWPGNVRELRHQISRAVLLSRNDRITAYDLAL
                     PMHVAKQNHPMQAMLNPGGMGRPAMSAGEGAFSASQMTLDSAERLLIENALKQANNNV
                     SEAARQLGITRMAMRYRMEKHGLKA"
     gene            complement(5277..>5479)
                     /locus_tag="VN084_04965"
     CDS             complement(5277..>5479)
                     /locus_tag="VN084_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28840.1"
                     /translation="ALLVAAALLSLSRLPKHYEAATIGATVGGVAGVVLATSTPIGLL
                     GGLVVGGWLGDQLNIGDTSANR"
BASE COUNT         1167 a         1660 c         1611 g         1041 t
ORIGIN      
        1 accacaccac gatcgacaac aaggacggcc tgttgccatc gagcgatccg gcgaccttga
       61 ccgcggagca tagctacaac cgcatcaatc ctgccatcgg cctgaatttc acgccttcct
      121 cggcgctgac ggtatacggc acctataacg aggccagtcg tgcgccgacc tcgatcgagc
      181 tgggttgcgc caatccggca cagccttgca agctgccgaa tgcgttcgcc ggcgatccgc
      241 cgctggacca ggtggtctcc aagacctatg agggcggctt gcgcgggcaa ttcgacaacg
      301 gcgtacgctg gagcacggcg gcttacgtga ccaacaacta cgacgacatc ctctttgccg
      361 cggcccctgg cgttgccggg cagggctact tcaagaactt cggccagacg cgtcgcaagg
      421 gcatcgaagc ctccttgctc ggcaacatgg agcgcttttc ctggagcgtc ggttatagcc
      481 tggtcgatgc acgctaccgc tcttccgaat ccatcgcttc tgaggtcaac agtgcggcca
      541 tcgacacgga tggtgacggc gaaggcgata ccttgcgcat tcgatccggc gatcgtatcc
      601 cgggcatccc catgcatcag ctgaagatgc gcggagtgtt cacggtgacg ccgaagtgga
      661 ccatcggtac cagcatcatc gccttcacgg accagtatgc ccgcggcaac gagaacaacg
      721 cgcaccggcc agataacgtc gatttcttcg gcgacggcaa ggtcagcggc tatgccgtgg
      781 tcaatctcga tacacgctat cacctgggca acggctggca agtctttgcc aaggccgtca
      841 atatcttcga ccgcgaatac aacaatgcgg gcctgctcgg cgcctccctg ttcaatgcca
      901 acggcagctt caatggtgac gaggatgccg gccgccaggc gctgtatgcc ccgggtgcgc
      961 cacgtgctgg ctggatcggc gtgcgctggg aattcggagg agccaagtcc agcggcatcg
     1021 ataccaacta gaaaggacag tggcgagatg gcgtacgcga caagtcaaga cggctgtgat
     1081 cggtcaggcg tgcgccactt gaatcgacgc tacgccacgt ttggctgaag acaacaacca
     1141 ccaatctggc tgtaattaac cagattggtg gttgtataca gccaattcat tcgctagact
     1201 ggcgagcggt gaggctttgt ctgcgctagg cccttgattg ttatatcgat cgtctcgatg
     1261 gacggcagtg gtacggaact tgattgacct caccagattg aatacaaaaa aatctgggga
     1321 gattgaaatg aagcgcgtcg tacctttccg cctgcgggcg gtcgtgggcc tggtggctgg
     1381 tttggccacc ttgtcggtca tggcagaaga ggtgagcgaa ccggacgctg gaaaaccggc
     1441 cagggccgag aagatcgagg tgatctcgac cacgcccttg ccagggatcg gtgcgccgat
     1501 cgaggagatc cccgcgaatg tgcagtctgc gtcctcgaag aacatcgagc cgcagcatac
     1561 ccaggatatc gccgattaca tcaacaccaa cctcggcagc gtcaccatca atgaagggca
     1621 gagtaatccg tatatgtcgg acgtcttctt tcgaggattc accgcttcgc cgttgccggg
     1681 agcgccgcaa ggcctttccg tgttccagga cggcgtacgg atcaacgagc ctttcggcga
     1741 taccgtgaat tggggattga tcccgcatac ggcgatcgcc agcatcaacc tcatgccggg
     1801 gtccaatccg atcttcggtc ttaataccct gggtggtgcg ctctcgatca acaccaagag
     1861 cggttcgcaa tatcctggcc tcgtcatgga agccagcggc ggttcatggg ggcgcaaggg
     1921 gttcaatgcg agttatggcg gcaagcaggg cgacatcgat tatttcgtta ccggtaattt
     1981 ctccagcgag gacggctggc gcgaacactc gccgagtcag gtcaaccagc tgttcggcaa
     2041 gttcggctgg caaaacgaca gcagcacgct cgacctgacg ctgagtgccg cccacaacaa
     2101 cctggaaggt gtccaggccc tgccgcgcag ctggctcaat acgccgaaac aggcctacac
     2161 ctatccggat tcagtgacct accgcatgca gatggccgcc ctcaagggta cgcatttcat
     2221 cgacgagaac aatctgctag cgggcaacat ctacgtgcgc aagagccgta tcggcaactt
     2281 cgccagtaac accaatgact gtttcggcag cacagacacc gcagctgggg aagagtgtga
     2341 ccagaccttg cctgatccga ccgatctcgc tacccagggc ttcaacgagc gctcgtccac
     2401 tgaccagctg ggctgggggt tcaccgggca gtattcctat ctggccgatc tcttcaagca
     2461 ccgcaaccag ttgaccacgg gtttcagttt cgaccatggc actacgcgct acaagtcggc
     2521 agagcaggat acggcgttca ccggccgcgc cgccacgccc ttgtcagatt tcgccgagga
     2581 aaccaatatc cgcggtgaga acagctatta cggcctgttc gcgaccgata cgttctccgt
     2641 caccgacaag ttccacctga ccatgtccgg ccgttacaac tatgtccgcg tcaagctcaa
     2701 ggactacatg gggccggatg acaataccgg caagtacgtc acgggggact ctcccggcat
     2761 cgtccatacc ttcaaccgcc tcaacccggc tgtcggcttc aactacaacc cgaacgatgc
     2821 catcggctac tatggcgcct ataacgaagg catgcgggcg ccgaccccga tcgaactggc
     2881 ttgtgccgac ccgttgacgc catgccgcct gccgaccgat ttcctggcgg atccctcgct
     2941 gaaaccggtg gtgtccaaga cctgggaagc cggcctgcgc ggcctcttca atagcgattg
     3001 gcgttggaat gccagcgtgt tccgctcgcg cctgaccgac gacatccagt tcatcagcag
     3061 cgaagcgacc aatttcggct acttcaagaa tgtcggcgaa acccgccgtc agggcctgga
     3121 gctcgggctg accggcaagg tcgagcgttt gcgtgtcgcc gccaattatc agtacctgca
     3181 agcgaccttc cagtcggcag tcaccttccg caacgagaac aactccagtg ccgatgcgaa
     3241 cggtaactac aggacgcggc caggcgacaa catccccaac atccctcgcc attcgctcaa
     3301 gctgcgcctg gcctatgacg tgacgcgtgc cctgacagtg ggcgtcaata ccatcaccgc
     3361 cagcagcatc tatgcccgtg gtgacgagaa caacaaggac gacaacggca agatcggcgg
     3421 ttacacggtc gtcaatcttg atgccaactg gcgactcaat cccaactgga cggcgttcac
     3481 acgggcagtc aatatcttcg atcgcgatta tcagacgctc ggggtgttgg gcgtgaacgc
     3541 attcaatacg gcagaccgct cattcaatac aggcggtacc gatagctggg tgcctgagca
     3601 attccgtacg ccggcgccgc cgcgtgccgc ctggttcggc ctgcgttacg agttcggtac
     3661 caatccgcag tcagcgagcg gctctgccga cccggattga acatcaaccc atgcatgacc
     3721 ctgatgaaag cgtgaccacg atttggtgaa gaacggatta tcgatgaatg cccggcgtat
     3781 cctcgttgtc gaggacgaga cgctattcgc gcgtgccgta tgcaagcggc tgcaaaaggc
     3841 tggctacgaa tgcgagcatg cggagaatct gcatgacggc cgtgcgatcg cgcggcagtt
     3901 cgtcccggat atcgccttgc tcgatatgcg cctaccagac ggtaacgggc tggacctgct
     3961 caccgagctt gtcgccaagg gcgtgacggt catcgtcatg actgcatacg gcgaggtcgc
     4021 cgatgccgtg agcgccgtca agcagggcgc catcgattac ctgaagaagc cggtcgatct
     4081 ggaagagttg ctgctggtga tccagaaagc cgagcagtcc accacactca agcatcatct
     4141 tgattattcc cgccagcgag atgcccatgc tgccgaaggc atcgaactgc tggggcaaag
     4201 tccggcgatg caaagcgtgc gcagccaggt cgagcgcatc tcgcagctgg tgacgatcga
     4261 gacggccgca ccgcccacgg tgttgatcaa tggcgaaacc gggaccggca aggatgtggt
     4321 cgcacgcttg ctgcatgtct cgtgcgccaa cagcgatcgc ccgttcgtgc atgtcgattg
     4381 cgcttccttg cccgcggacc tgatcgagag cgagttgttc ggacacgaga agggcgcctt
     4441 cactagtgcc caaggtgcga ggccaggtct gatcgaggcc gccgaagacg gtacgctgtt
     4501 cctggatgaa ctgggcgagt tgcccctggc cttacaggcc aagctgctca acgtgctgga
     4561 acgtcgtgtc gtgagacggc tcggctcgac acgtgaacgg gccgtgccgg cacggttcgt
     4621 ggccgcgacc aaccgcgatc tgcatcagat ggtgctcgat ggtcgtttcc gttctgatct
     4681 ctactaccgg ctcaacgtat tgaccatcct tatgccgccg ttgcgtgaac gggggggcga
     4741 tgtgctgctg ctggcaaggc acttcgctgc gcagacagaa cgacgttatg ggttgccaca
     4801 gcgggatttc gcggcagacg ccatcgagat gatgggtaat tatccctggc ccggcaatgt
     4861 ccgggaattg cggcaccaga ttagccgtgc tgtcctgttg agccgtaacg accggatcac
     4921 ggcttatgat ctggcgttac ccatgcatgt cgccaagcaa aaccatccga tgcaagcgat
     4981 gttgaaccct ggcggcatgg gccggccggc catgagcgcg ggggaaggtg ccttcagcgc
     5041 ctcgcagatg acactggatt cagccgagcg cttattgatc gagaatgcgc tcaagcaagc
     5101 caataacaat gtgtcggaag ccgcacgcca gctcggcatc acgcggatgg ccatgcgtta
     5161 ccgcatggaa aagcacggcc tcaaggcctg atccgtctcc gattggagac ggatttccaa
     5221 tgctttagta aaaatcctgt aaatacagaa gcttgaggtg tctctcggcg acgacgctaa
     5281 cgattggcgc tggtgtcgcc gatattcagc tggtcgccca gccagccccc gaccaccagc
     5341 ccgccaagca agccgatggg tgtgctggtc gcgagcacga cgccagcgac gccacctaca
     5401 gtagcgccga tggtagcggc ttcatagtgt ttcggaagcc tggaaaggct cagtaaagcg
     5461 gcggcgacca gtaacgcga
//
LOCUS       DATMTW010000252         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_458504, whole genome shotgun sequence.
ACCESSION   DATMTW010000252 DATMTW010000000
VERSION     DATMTW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458504"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..912
                     /gene="dapA"
                     /locus_tag="VN084_04970"
     CDS             40..912
                     /gene="dapA"
                     /locus_tag="VN084_04970"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWT28841.1"
                     /translation="MLQGSLVAIVTPMHQDGSLDLKALRTLIDFHVTEGTDGIVIVGT
                     TGESPTVNFDEHCLLIKTAVEQSAGRVPVIAGTGANSTTEAIELTSAAKSLGVDACLL
                     VAPYYNKPSQEGLYQHFRTVAETVDIPQILYNVPGRTGCDIVNDTVLRLVQLPNIVGI
                     KDATGNIERGTDLLLRAPDDFAVYSGDDATAMSLMLLGGRGVISVTANVAPRLMHELC
                     VAAMAGDAKTARGINAQLFGLHQKLFVEANPIPVKWVLEQMQLIGPGIRLPLTPLATQ
                     HHETLRRAMRQAAL"
     gene            949..2220
                     /gene="bamC"
                     /locus_tag="VN084_04975"
     CDS             949..2220
                     /gene="bamC"
                     /locus_tag="VN084_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HWT28842.1"
                     /translation="MNRTLNQTLVATLLALSVAGCDSIPFVDNTPDYKSAGRSRPLEV
                     PPDLTSASASDTYSVPGGSTTYSSYSQSQAEQGEAQEKILPNPDNVRLERAGSQRWLV
                     VDAPAEKIWPVIREFWAELGFAVRVENPQTGVMETEWVDPSSLTKDEKGNYLDKFQGW
                     LDKLNSLQTRQKFRTRIDRGVEKGTTEIYLSHRSISDSVDDGKERVLTSVGTVEQGYK
                     LDKRTKQDEVRADAEDIDAELLRRLMVRLGVEEKSSRKVMAAVSNEVRATLSKNSDNS
                     LSIGLKDPFDRAWRRVGLALDRVGFVVEDRDRSSGIFFVRYSDVGIDDAPKKEEGLLD
                     KLKFWGDDDKKPKEEDIPEPKKKNDEGVIDKLKFWEVKDKDKAPPERQYRVKVVADDN
                     GSTVLVTDKAGQRNTSTTANRIISLLYEQLK"
     gene            2275..>2462
                     /locus_tag="VN084_04980"
     CDS             2275..>2462
                     /locus_tag="VN084_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWT28843.1"
                     /translation="MRFASLGSGSNGNGLVVEKRTTRLLMDCGFGLRDVVQRLARLAL
                     SPADITGILVTHEHDDHAG"
BASE COUNT          562 a          763 c          707 g          430 t
ORIGIN      
        1 gttaaaataa tccgatttat attctctgtc aggaacatca tgctgcaagg cagtcttgtc
       61 gccatcgtca cccccatgca ccaggatggc agcctcgatc tgaaagcatt gcgcacgctc
      121 atcgattttc atgtcaccga ggggacggac ggcatcgtca tcgtcggcac gacgggcgaa
      181 tcgcctacgg tcaatttcga cgagcactgc ctgctgatca agaccgctgt cgagcagagc
      241 gcggggcgcg ttcccgtgat cgccggcacc ggcgccaatt cgaccacgga agcgatcgaa
      301 ctcaccagcg ccgcgaaaag cctgggagtg gatgcctgcc tgctggtggc gccttactac
      361 aacaagcctt cacaagaagg cttgtatcag cacttccgta ccgtcgccga aaccgtcgat
      421 atcccgcaga tcctctacaa cgtgccgggg cggactggat gcgacattgt caacgatacc
      481 gtgctgcgcc tcgttcagct tcctaacatc gtcggcatca aggatgcgac aggcaacatc
      541 gagcgcggca ctgacctgtt gttacgcgcg ccagacgatt tcgccgtcta cagcggcgac
      601 gatgccaccg ccatgtcatt gatgctgctg ggcggtcgcg gcgtgatctc ggtcactgcc
      661 aacgtcgcgc cgcgactcat gcatgaacta tgtgtcgccg ccatggctgg cgatgcgaag
      721 acggcgcgcg gcatcaatgc ccagctgttc ggcttgcatc agaagctgtt cgtcgaggcc
      781 aatccgatcc cggtgaagtg ggtgctggaa cagatgcaac tgatcggccc cggcatccgc
      841 ctgccgctga cacccttagc gacgcagcat cacgagacct tgcgtcgcgc catgcggcaa
      901 gcggcgttgt aacaccaggc aacaccactc atcctcactg agagacgtat gaatcgaacc
      961 ttgaaccaga cccttgtcgc caccctgctc gcactgagcg ttgccgggtg cgactccatc
     1021 ccctttgtcg ataacacgcc ggattacaaa tcggctggcc gctcgcgccc actggaagtc
     1081 cccccggacc tgacctccgc atcggccagc gacacctact ccgtccctgg cggctccacc
     1141 acttattcca gttacagcca gtcgcaagcc gaacaaggcg aggcccagga aaagatcctg
     1201 cccaacccgg ataacgtcag actggaacgc gccggctcgc aacgctggct ggtggtggac
     1261 gctcctgccg agaagatctg gccggtgatc cgtgagttct gggccgaact gggcttcgcg
     1321 gtgcgggtcg agaacccgca gacgggcgtc atggaaaccg aatgggtcga cccttcgagc
     1381 ctgaccaagg atgaaaaagg caactacctt gacaaattcc agggttggct cgacaaactc
     1441 aactccctgc agacccgcca gaaattccgc acgcgcatcg accgtggcgt cgagaaaggc
     1501 acgaccgaga tctacctcag ccatcgctcc atcagcgaca gtgtggatga cggcaaggaa
     1561 cgcgtactga ccagcgtcgg cactgtcgag caaggctata agcttgataa acgcaccaag
     1621 caggatgaag tacgcgcaga tgccgaagac atcgacgctg aattgctgcg ccggctgatg
     1681 gtccgcctgg gcgttgagga aaagagctca cgcaaggtca tggctgcagt cagcaatgaa
     1741 gttcgcgcta cgctgagcaa gaacagcgac aacagcctca gcatcggcct caaggatccg
     1801 ttcgaccgcg catggcgccg tgtcggcctg gcgctcgacc gtgtcggttt cgtcgtcgaa
     1861 gaccgcgacc gttccagcgg catcttcttc gtccgttact ctgacgtcgg tatcgacgac
     1921 gcgccgaaga aagaggaagg cctgctcgac aaactcaaat tctggggcga tgacgacaag
     1981 aagccgaaag aggaagatat ccccgagccc aagaaaaaga acgatgaagg cgtcatcgac
     2041 aagctcaagt tctgggaagt gaaggacaag gacaaggcac cgccagagcg ccagtaccgg
     2101 gtcaaggtcg tcgcggatga taacggcagc acggtgctgg tcaccgacaa ggctggtcaa
     2161 cgcaacacct cgacgaccgc caaccgcatc atcagcctgc tctacgagca actgaaataa
     2221 ccgcctaaac atgcggctgg tcacgcggcc agccgtcaag gacagggagt gaccatgcgt
     2281 tttgcctctc tgggcagcgg cagtaatggt aacggcttgg tcgtggaaaa acgcacgacc
     2341 cggctactga tggattgcgg attcggcctg cgcgatgtgg ttcaacggct ggcaagactg
     2401 gcgctaagcc cggctgacat caccggcatc ctcgttacgc acgaacatga cgatcacgct
     2461 gg
//
LOCUS       DATMTW010000253         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_56550, whole genome shotgun sequence.
ACCESSION   DATMTW010000253 DATMTW010000000
VERSION     DATMTW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56550"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2159
                     /gene="pnp"
                     /locus_tag="VN084_04985"
     CDS             <1..2159
                     /gene="pnp"
                     /locus_tag="VN084_04985"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWT28844.1"
                     /translation="QASGAVLVNIDDTVVLATVVASKGAKPGQDFFPLTVDYIEKTYA
                     AGKIPGSFFKREAKPSEHETLTSRLIDRPIRPLFPEGFFNEVHVVIHTVSLNPEVDAD
                     IAALIATSAALAISGIPFNGPIGAARVGYINGEYVLNPGQTARKNSQMDLVVAGTEAA
                     VLMVESEAQQLSEEIMLGAVVFGHEQGNVAINAIHELVRDAGKPVWQWEAPAKDETLI
                     AKVVALADDKLRAAYQIRNKQARTQACREAYAAVMAALKAEGVEFDAVKVEGMLFDIE
                     AGIVRSQILAGEPRIDGRDTRTVRPIEIRGSVLPRAHGSSLFTRGETQALVVTTLGTE
                     RDAQRIDALAGEYEDRFMMHYNMPPFATGEVGRMGSTKRREIGHGRLAKRALIAVLPT
                     KEEFPYTMRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKDANRF
                     AVLTDILGDEDHLGDMDFKVAGTTGGITALQMDIKIQGITKEIMQVALAQAKEARMHI
                     LGKMQEAMGEAKTEVSNFAPKLYTMKINPEKIRDVIGKGGAVIRALTEETGCQINIEE
                     DGTITIAATDAAKADEAKKRIEQITAEVEIGKIYEGPVTKILDFGALINLLPGKDGLL
                     HISQIAHERVERVGDYLTEGQIVKVKVMETDEKGRIKLSMKVLADRPAGGSDRPAPAE
                     RGDREPRRDHGRDGGRHPAEQQQQQSLPADGASAQV"
     gene            complement(2810..2992)
                     /locus_tag="VN084_04990"
                     /pseudo
     CDS             complement(2810..2992)
                     /locus_tag="VN084_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
     gene            3511..3834
                     /gene="clpS"
                     /locus_tag="VN084_04995"
     CDS             3511..3834
                     /gene="clpS"
                     /locus_tag="VN084_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWT28845.1"
                     /translation="MSGDQGRPTGPTTSVITKVKPKTKRPSLYRVLILNDDYTPMEFV
                     VHVLEKFFQKDAEAATEIMLHVHHHGIGECGVFTYEIAETKVTQVMDFARKHQHPLQC
                     VMEKK"
     gene            4091..>5323
                     /locus_tag="VN084_05000"
     CDS             4091..>5323
                     /locus_tag="VN084_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWT28846.1"
                     /translation="MHRALAIANERHHQYATLEHLLLSLVDDSDAAAVMRACSVDLDK
                     LRSSLVNYLETEFENLVTDGSDDAKPTAGFQRVIQRAVIHVQSSGREEVTGANVLIAI
                     FAERESHAAYFLQEQDMTRYDAVNYISHGIAKRPGVSEARPIRGVDEEAESKNSDDAK
                     KKGEALDTYCVNLNKKARDGKIDPVIGRNAEISRAIQVLCRRQKNNPLFVGEAGVGKT
                     AIAEGLAKRIVDSEVPEVLSAATVFSLDMGTLLAGTRYRGDFEERLKQVLKELEAHPN
                     AILFIDEIHTVIGAGATSGGAMDASNLLKPALASGTIRCMGSTTYKEYRQHFEKDRAL
                     VRRFQKIDVNEPTVEDAIAILKGLKPYFEDYHKLKYTNDAIEAAVQLSSRYIHDRKLP
                     DKAIDVIDESGAAQMLVAE"
BASE COUNT         1093 a         1579 c         1659 g          992 t
ORIGIN      
        1 gccaagcgtc cggcgctgtg ctggtgaaca ttgatgacac cgtggtgctg gccaccgtgg
       61 tggcatccaa gggtgccaag ccaggacagg acttcttccc cctgacggtg gactacatcg
      121 agaagaccta cgctgcaggc aagatccccg gcagcttctt caagcgcgaa gccaagccca
      181 gtgagcacga aaccctgacc agccgtctga tcgaccgccc gatccgcccg ctgttccccg
      241 aaggtttctt caacgaagtg catgtggtca tccacacggt gtcgctgaac cccgaagtgg
      301 acgctgacat cgcagccctg atcgctacca gtgcagccct ggccatctcc ggcatcccgt
      361 tcaacggccc catcggcgcc gcacgcgtgg gctacatcaa cggcgaatat gtgctcaacc
      421 ctggtcagac tgcgcgcaag aactcgcaga tggatctggt ggtggcgggt accgaggccg
      481 cagtgctgat ggtggaatcc gaagcacagc aactgtccga agaaatcatg ctcggcgccg
      541 tggtgtttgg ccacgagcaa ggcaatgtgg ccatcaacgc catccacgaa ctggtgcgcg
      601 atgctggcaa gcccgtatgg cagtgggaag cccctgccaa ggacgaaacc ctgatcgcca
      661 aggtggtggc tctggccgac gacaagctgc gcgctgccta ccagatccgc aacaagcaag
      721 cccgcacgca ggcttgccgc gaagcttatg cggccgtgat ggcagccttg aaggctgaag
      781 gcgtcgaatt cgacgctgtc aaggttgaag gcatgctgtt cgatatcgaa gccggcatcg
      841 ttcgcagcca gatcctggct ggtgagcccc gcattgatgg ccgcgacacg cgcaccgtgc
      901 gccctatcga aatccgtggc agcgtactgc cccgcgccca tggctcgtcg ctcttcacgc
      961 gtggcgaaac ccaggcgctg gtggtgacca cgctgggcac cgagcgcgac gctcagcgca
     1021 ttgatgcgct ggctggcgag tatgaagacc gcttcatgat gcactacaac atgcctccct
     1081 ttgccaccgg cgaagtgggc cgcatgggtt cgaccaagcg ccgcgaaatc ggccacggcc
     1141 gtctggccaa gcgcgctctg attgccgtgt tgccgacgaa ggaagaattc ccctacacca
     1201 tgcgcgtggt gtccgaaatc accgaatcca acggctcgtc gtcgatggca tcggtctgcg
     1261 gtggctgcct gtcgctgatg gacgcgggcg ttccgatgaa ggcgcatgtg gcgggtatcg
     1321 ccatgggcct gatcaaggat gccaaccgtt ttgccgtatt gaccgacatc ctgggcgatg
     1381 aagatcatct gggtgacatg gacttcaagg tggcgggtac cacgggtggt atcacggccc
     1441 tgcagatgga catcaagatc cagggcatca ccaaggagat catgcaggtc gccctggccc
     1501 aggccaagga agcgcgcatg cacatcctgg gcaagatgca agaggccatg ggtgaagcca
     1561 agactgaggt gtcgaacttt gcgcccaagc tctacacgat gaagatcaac cccgagaaga
     1621 tccgtgacgt gatcggcaag ggcggcgcgg tgatccgtgc gctgaccgaa gaaaccggct
     1681 gccagatcaa catcgaggaa gacggcacca tcaccatcgc cgccactgac gcagccaagg
     1741 ccgacgaggc caagaagcgc atcgagcaga tcacggccga agttgaaatt ggcaagatct
     1801 acgaaggccc tgtgaccaag atcctggact tcggcgcact gatcaatctg ctgcccggca
     1861 aggatggtct gctgcacatc agccagattg cccacgagcg cgttgagcgc gtgggtgact
     1921 acctgaccga aggccagatc gtgaaggtca aggtcatgga aaccgacgag aagggccgca
     1981 tcaagctgtc catgaaggtg ttggcagatc gtcccgccgg cggcagtgac cgtcctgcgc
     2041 ctgctgagcg tggcgaccgc gaaccccgcc gcgaccacgg ccgtgacggt ggacgccatc
     2101 ccgctgagca gcaacagcag cagtccctgc ctgcagatgg tgcctctgcc caggtatagt
     2161 tggcgagttt ccaggttccg ccgttgttag tgcatttgtt ctttgtggta tatcgtgaat
     2221 ctgagcaggc ttgtgcagac gtgcaggtgc tcttggtttt atagctcaag ccgccgctgc
     2281 aagccccgtt gttctcgtcc caactatcgc ctgacgggct gccgctccac ttgatccagc
     2341 tcgcgttgta gttggacgaa ccgacgttca cgtccgtcga gaacggaacg atggaaatat
     2401 agacatcgcc gttctgtttg ctgaatccgg cgagggagtc gatcaagtct ttcgcggccg
     2461 tctgcagcgc cggcatcttg ccgttttcgg ccatcgaccc ggtgttatcc agcaccaagg
     2521 ccacgcgcat acgggtgttg ccccatgcgg ttttcgagtc cgctccgatg tcgagctgcg
     2581 gcaatccgac gagcttcatg aaatcggtcg gaacaattcc gctgccttcc atcgtgatgc
     2641 tggcgggcgt gccgctgctg ccggccgaat agaccgcttt caccgcgatg ctgttggcat
     2701 cgctgttatg ataaagggca tcgaaatact gcttcgccag gttctgaatg gcgtccgctg
     2761 aaaggctggg gttcgctgcg gcgtccctgg agatcatcag ggacgcggtg tcgagcgccg
     2821 cctgcatggc ggagcgggcg gcactggccc gggtgtagtc caccgccgcg ccgacgatgg
     2881 cgaggagtgg aacgagggtg atcgcaaaga tcatggcgat attgccctga tcggcgcggg
     2941 caaatcgctt cgccgcgctg cgcatcgaac ctggaattgc tttaccgaac atggctgtca
     3001 ccagttgtgg gttacgaggc catttcgtgt ggcacgacta aacaggtggt aaagccgtgc
     3061 tgagaaagca ggtaaataac cggacaaatt gcgaatgacg ccgttccggc tgctgtcacg
     3121 gtcaacagac gctaaatggt cgacgtgctg ttttcggcca accaggcgag ggttgattaa
     3181 ccgtcgaccg ggcggcgaga cgctttcggg ccgcgttgcc ggatggggat gagggcatct
     3241 gcctgcttgc ggcagggccg gcccagaccc atattggcac ggatcgtcga tccggccgcc
     3301 cgaccgcggt gggtccggga agcagactga aacgggactt gccgggggcg gatgtggtag
     3361 ctgccggcaa tagtcagacg ttgatcgtca gcttccttct tcgttttggg atttcgaaca
     3421 aactgagcca tgtcgcaatt gtctttgaat tccggtctgt ccgacacgcc cgaggccgct
     3481 ctgcttgagg ctgccatgcc gtcaccccga atgagtggcg atcaagggcg tccgaccgga
     3541 ccgacgacct ctgtcatcac caaggtcaaa ccgaagacga agcggcccag cctctatcgc
     3601 gtgctgatcc tgaatgacga ttacacgccg atggagttcg tcgtccatgt actggaaaaa
     3661 ttcttccaga aggacgccga ggcggcaacc gagatcatgc tgcacgttca ccatcacggc
     3721 atcggcgagt gcggcgtttt cacctacgag atcgccgaga ccaaggtgac ccaggtgatg
     3781 gacttcgcgc gcaagcatca gcatccgctc cagtgcgtga tggagaagaa gtagcgcgcc
     3841 ggtcgatatg acgcaggcca agtattctgc cgcaggcgtc tcgtgattcg gctgcgcagg
     3901 agttgatttc cggcttgaat cttgccggat ttgcggctcc gaattctcgg ctggaaccgc
     3961 tcttccgcca ttcccgaacg taactatatg tggaaaggtt gttgttgcct gttcgggtaa
     4021 ccgcgatcat gatggctggg ggcaatagag gactcgaatg ccaacttttt ctcagagcct
     4081 tgaacaatct ttgcatcgtg cgctcgcgat cgcgaacgaa cggcatcatc agtatgcgac
     4141 gctcgagcat ctgttgctgt cgctggtcga tgactccgat gcggcggctg tgatgcgcgc
     4201 atgcagtgtc gatctcgaca agctgcgctc cagcctcgtc aactacctcg agaccgaatt
     4261 cgagaacctg gtgacggatg gttcggacga tgccaagccg accgcgggct tccagcgcgt
     4321 gatccagcgc gcggtgatcc acgtgcagtc gtccggtcgc gaagaggtga ccggggcgaa
     4381 cgtgttgatc gcgatcttcg ccgagcgcga aagtcatgcc gcctacttcc tgcaggagca
     4441 ggacatgacg cgctatgacg cggtgaacta catcagccac ggcatcgcca agcgtcccgg
     4501 cgtttccgaa gcgcgcccca ttcgcggcgt agacgaagaa gccgaatcca agaacagcga
     4561 cgacgccaag aagaagggcg aggcgctgga tacctattgc gtcaacctca acaagaaggc
     4621 gcgtgacggc aagatcgatc cggtgatcgg ccgcaatgcc gagatcagcc gcgcgatcca
     4681 ggtgctgtgc cgccgccaga agaacaatcc gctgttcgtc ggcgaggcgg gcgtcggcaa
     4741 gaccgcgatt gccgaaggtc tggccaagcg catcgtcgac agcgaagtgc cggaggtgct
     4801 gagcgccgcg acggtgttct cgctcgacat gggcacgctg ctggccggta cgcgctatcg
     4861 cggtgacttc gaggagcgcc tcaagcaggt gctgaaggaa ctcgaagcgc accccaacgc
     4921 catcctgttc atcgacgaga tccataccgt gatcggtgcg ggtgcgacct ccggcggcgc
     4981 gatggatgcg tccaacctgc tgaagccggc gctcgcatcg ggaaccattc gctgcatggg
     5041 ctcgacgacc tacaaggaat accgtcagca cttcgagaag gaccgcgcgc tggtgcgccg
     5101 gttccagaag atcgacgtca acgaaccgac ggtcgaagat gcgattgcga tcctcaaggg
     5161 cctcaagcct tacttcgagg actatcacaa gctgaagtac accaacgacg ccatcgaggc
     5221 ggcggtgcaa ctgtcgtcgc gttatatcca tgaccgcaag ctgccggaca aggcgatcga
     5281 cgtgatcgac gaatccggcg cggcgcaaat gctggtggcg gag
//
LOCUS       DATMTW010000254         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_390812, whole genome shotgun sequence.
ACCESSION   DATMTW010000254 DATMTW010000000
VERSION     DATMTW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390812"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..735
                     /locus_tag="VN084_05005"
     CDS             337..735
                     /locus_tag="VN084_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWT28847.1"
                     /translation="MYNLILLTNILRILDDIGMTKSELAKKAGMSVSFLSDLTNDQAN
                     PSLRIMEAIAHALDTPLPALLETTDLDRQTLEELAGGKSRLSLPEGYARVAAILTEFQ
                     AFTVRQWDQENRKRLDAAKVRRKTVPAKKT"
     gene            950..1912
                     /gene="trbB"
                     /locus_tag="VN084_05010"
     CDS             950..1912
                     /gene="trbB"
                     /locus_tag="VN084_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HWT28848.1"
                     /translation="MTERDDIVATIKTRAKERLMRDMGPELLAVLANPRTVEIMLNAD
                     GCLWQEQLGEGMRRIGEMRPAKAEAIIKTVAAALDQVVNKAHPLVEGELPLDGSRFAG
                     QLPPVVSAPTFAIRKRAIAVFTLDQYVEAGIMTARHCQALKGAVRQHRNILVTGGTGS
                     GKTTLVNAIINEMVSANPDERVCIIEDTGEIQCTATNCIQYHTALDVPMTTLLKIILR
                     MRPDRILVGEVRGAEALDLLDAWNTGHEGGAATLHANSALAGLTRLKSLITRNEAAPS
                     EIEPLIGEVVHLVLHIARVPEGRRVQEIHEISGYAAGRYLSKTL"
BASE COUNT          440 a          593 c          546 g          400 t
ORIGIN      
        1 ctggagaagg ggctgtctat cgccacctga gaagagcggc gagggacacg tcgtttcgat
       61 atttcgaagt agaccctagt atcatcatga tgtcttgttt gcggaagtta aacgggtcga
      121 ttatttcgga tacatcaaac ggtcggcacg gcaggcggcg aagcgacgtc accagcgtgc
      181 taacgattag ggctactgat cagagaagta gttctgcggt acactgaaac gaccaaaagc
      241 gcctacacta gagcagcact ctgagtgctc tcgcgtccaa cttattaggg gtagcgaact
      301 ttagcggcta aatttgcttg ccctatcgga gcctgtgtgt acaacctcat cctcctaacg
      361 aacatcctgc gcatcctcga cgacatcggg atgaccaaga gcgagttggc aaaaaaggct
      421 ggcatgtcag tgtcgtttct ctccgacctg accaacgacc aggccaaccc gtcgctgcgc
      481 atcatggagg cgatcgctca cgcactggac acgccgctgc ccgcgctgct tgaaactacc
      541 gatctggaca ggcagacttt ggaggagctc gcgggtggta aatcaaggct gagtctccca
      601 gaaggatacg ctcgcgtcgc agcgattctt accgaattcc aagcattcac ggtcaggcag
      661 tgggatcagg agaaccgcaa gcgtcttgat gccgcaaaag tacggcggaa gactgtccct
      721 gccaagaaga cctagtccac gcagtcgtcg attccgaaag cattagcgag agtcgcggtc
      781 cgtttttcga actggccggt tttcgctgat cctttacgct aggcaacccg tttgagatca
      841 cgcaagcaaa ttcggtttag cgtagcgacc cacacatcct ttgttctaga tcgtttcggt
      901 atatccccgg taacgtagca tcgctcggca taacctagag tgagcaatca tgaccgagcg
      961 cgatgacatc gttgcaacca taaagacccg tgccaaggaa aggttgatgc gtgatatggg
     1021 gccggaactc ctggccgtgc tcgccaatcc ccgtacggtt gaaatcatgc tcaacgctga
     1081 cggctgcctg tggcaggaac agcttgggga gggaatgcgc cgcattggcg agatgcggcc
     1141 ggcaaaggcc gaagccatca tcaagaccgt ggccgcagcc ctggatcagg tggtcaacaa
     1201 ggcccatcct ctcgtcgaag gcgagcttcc gctcgatggt tcgcggttcg ccggccagct
     1261 tcctccagtc gtctcggcgc ccacctttgc aattcgcaag cgcgccatcg ccgtcttcac
     1321 gctcgaccag tatgtcgagg ctggaatcat gacggcgcga cattgccaag cccttaaagg
     1381 cgccgttcgc cagcatcgga atatcctcgt caccggcgga accggttccg gcaagaccac
     1441 gcttgtcaac gcgatcatca acgaaatggt gagcgcgaat ccggacgagc gggtgtgcat
     1501 tatcgaggat actggcgaaa tccagtgcac ggccacgaac tgcatccagt accacacggc
     1561 tctcgatgtc ccgatgacga cgctgctcaa gatcatcctg cgcatgcgcc cggaccggat
     1621 actggtcggc gaagtacgcg gcgccgaggc gctcgatctt ctcgacgcct ggaacaccgg
     1681 acatgaagga ggtgccgcaa cccttcatgc caatagtgct cttgccggcc tcacgcgtct
     1741 gaaatcgctc atcacccgca acgaggccgc accttccgaa atcgaacccc tgatcggcga
     1801 ggtcgtccat ctcgtcttgc atatcgcccg tgttcccgaa ggccgccgcg tacaggagat
     1861 ccacgagatc tccggttacg cggcaggccg ctatctctct aaaaccctct aggagtgtca
     1921 aatggagccg atccgttttg ccccgcagtc ccggtttacc ctcgccttgc tgccttacc
//
LOCUS       DATMTW010000255         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_390877, whole genome shotgun sequence.
ACCESSION   DATMTW010000255 DATMTW010000000
VERSION     DATMTW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390877"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN084_05015"
     CDS             complement(<1..389)
                     /locus_tag="VN084_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HWT28849.1"
                     /translation="MNRYKSAPIQRKGLSVAIARDNLLYTTEALLDPLVIVLTLWSVA
                     YLKNGGLEPHYLILSLILFSLTFPSASKINLTSLEVVSTVMLNWVIIAGLLILFGTAT
                     DYIALFNTEAILMWTWLTPLCVLTADFA"
     gene            complement(376..1164)
                     /gene="epsA"
                     /locus_tag="VN084_05020"
     CDS             complement(376..1164)
                     /gene="epsA"
                     /locus_tag="VN084_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HWT28850.1"
                     /translation="MPLHTELDEVQKTQFFGMIQESLSVRTHFEFMLWSQGNVQAFLP
                     HDIMIAAWGDFSLGLISFDVVSPLPGMRTQQVQHARLVPLLKRLFNYWHEQGDAPLSM
                     RMQHGLFSENQFGFDQLGEDFARMKSGMIHGIKDSRGRHDCLYILLNASDDLSQASRT
                     MLETLLPYVDCTLRQIEPIAVEPPKPVTTSIDEGFGTLSIREHEIMDWVKQGKTNYEI
                     GMILDISAFTVKNHLQRIFKKLDVLNRAQAVAKLSMMPEPNESV"
     gene            1763..2266
                     /locus_tag="VN084_05025"
     CDS             1763..2266
                     /locus_tag="VN084_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWT28851.1"
                     /translation="MIAPYSDIEPLGRADRYIDEIMEIVHQIDLFESLSAPEILAVCR
                     HMDCYAAPRGYSLLRQDATDDHLVLLLSGTAVIKRDSLLDGEEIIAEVSAGTLLGEMS
                     MMDSKPHLTSCVAITPLDFAVFSRDTLNTILVNTPRLGNKLLLTMLHLVSARLRDASY
                     RFLPSLA"
     gene            2308..3375
                     /locus_tag="VN084_05030"
     CDS             2308..3375
                     /locus_tag="VN084_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWT28852.1"
                     /translation="MIKSFTKLLGLFLLVCILAYLPSSYAASNDYQLGAGDAVRISVF
                     QNPDLSLETRVSESGSISYPLIGEVQVGGLSPREAEQLIAKKLMDGNFVKRPQVNLTL
                     VEVRGSQIAVIGLVNKPGRFPVESAHMRLSEAIAAAGGVSDAAGTGTAVVTGKRDGRP
                     MRVETDIASLFLSDGGGQDTELKAGDIIYVMTGNQVSIQGQVNRPGRYALESNKMHVS
                     EVLTLAGGLTPDAADVAIVAGKRNGENFRKEIDIPALYLSDSNDQNLLIAAGDEVYVQ
                     KAPVYYIYGEAQKPGSYRIERNMTVIQALAQGGGPTIRGTQRNLKLMRRDSHGETVEL
                     TPKLTDLVRPDDVIYVRESLF"
     gene            3385..4791
                     /gene="epsF"
                     /locus_tag="VN084_05035"
     CDS             3385..4791
                     /gene="epsF"
                     /locus_tag="VN084_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HWT28853.1"
                     /translation="MSLTNLFLILKARIKIVVFIFVVTVLTAFVASMMMPKYYKASTQ
                     VVVNYRGNDSVTGNSNQSLMTSSYLATQVDIIKNRSVALSVVDALQLVDNDAMKKDFY
                     EVTKGRGDIRQWIAGRLISRLEVSPLRESSVMEITFSGKDPQQVAEIADAFAEAYLDR
                     TLRLKIEPVKKAANFFNQQMTTLRDSLEKAQSKLSQYQQTHGITSTDQRLDIETAKLN
                     ELSSQLVAAQASAIEANSRYGNSRKDAGNSPDVAQNPVIQNLRVEVARASAKVADLSE
                     RLGSNHPEYEAAAAELKNVQSQLYTEIARANNNIKGSAIIQQQRVEELRSALAKQKLA
                     VLALNRQRDEMAVLQEDVETAQKALDAVTQRFTQTSIEAQSNQNDIAILSAAQVPGSP
                     YTPRIMLNMLLAMFVGGVLGLGFGLIAEMLDRRVRSSDDIAELLKVPVVALVNKRPAV
                     TGMKMLPGAQSGKFFPST"
     gene            4799..5701
                     /gene="epsG"
                     /locus_tag="VN084_05040"
     CDS             4799..5701
                     /gene="epsG"
                     /locus_tag="VN084_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HWT28854.1"
                     /translation="MNISQLNSPPIPLGAERDQGRQESERKAPLGQLLLKMGKLTEDE
                     IERVLALQQQTGLRFGAAAKKLGLVSETDIQHALAVQFDYHYLHKSQEFFSKRLIAAY
                     DPFSPQVEALRALRSQLLLRWFDDAEKVLAVVAANPGEGCSNLAANLAVVFSQLGERT
                     LLIDANLREPDQRNIFNLYESRGLSDILAGRADLDVIHKIDSLSGLSVLGAGTVPPNP
                     QELLSRKVFGEVLRRCREQYDVILVDTYPASMTSDAQTVAARCGGALLVSRLNQTRMA
                     QLANVRDQLLMSEVEIVAAIVNDL"
     gene            5738..7045
                     /locus_tag="VN084_05045"
     CDS             5738..7045
                     /locus_tag="VN084_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWT28855.1"
                     /translation="MTALLKSSGTTLVTTIVITAIGFITSVITARVLGPEGRGLLSGA
                     LLIATLAGNVALFGLANSYIYHKGTGRPFRYGLFFAGSLLFVAGLSALLGLIGTQVMT
                     HEAQLHAQLPLILLLTVVTATQGYFYALSQLHHGLRFFNLMRFLLVIGNLILLLGLLL
                     FFKPVDFRMILVTQTIVSAGLTLAGIAWARHHRIWEGSDTTQAPVAWKSMLGYGASHH
                     GTVVIGLLLVNFDKVALLNIGTIVEYGFYALAFTTSRLIGALQEAVSTALYSRHAGRD
                     VQVLSENVRTAFRMTFVPMLAVAAIGALLSPWLIVLVYGESFASTVIPFAVLLFECVI
                     SGASWTLAQRFNAGGRPGLVFVRQFISVLPIFIALPFLPHENIHVYLSLLMLAGGILR
                     LSVTLVIYPTVLKERVPDILPTLADCRAVFNVLARRQVADGKA"
     gene            7042..8043
                     /locus_tag="VN084_05050"
     CDS             7042..8043
                     /locus_tag="VN084_05050"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HWT28856.1"
                     /translation="MNRTDSHQALKHPVVNHALLMGALATQHDVLLDLIGNRPLHYVD
                     LPVHGNIGDLLIMLGTLAFFQHNYLVPKVSAAAFAYEPSWIAPGDVVVFHGGGNFGDL
                     YAEYGMQGLREQVADRYRDNRIVVLPQTLYFSSQAAMRRSAAVFRRHPDLHLCVRDRI
                     SYDIARNFTDNVYLLPDMAHQLYRLTSRHDAPHGTLRIQRTDDERAEAGAMPGLEIVT
                     TTDWPMVVGDGERRIALFRRGMRALHKYGLGWYGNKLLAGLWADYARHLVQQATTLFA
                     AHEHIVTDRLHGHILACLMDKPNTVLDNSYGKNATYVNAWTGNSELVTLQAASAEKI"
     gene            8085..>8607
                     /locus_tag="VN084_05055"
     CDS             8085..>8607
                     /locus_tag="VN084_05055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWT28857.1"
                     /translation="MATFDVLLPVKNGIDYLGEAIDSICQQTFRDWRLLILDHGSNDG
                     SLELAQRYAGQDPRVEVHCLPSAQGLAGLLNAGLDLCHGKYVLRQDADDISLPNRMQV
                     LFHALEEDDELVLVGSLGDVVDADGNKIGILDMPTGQFGVLISTPFRTPVAHPTVAMR
                     LDAIRGLNARYGVD"
BASE COUNT         1663 a         2642 c         2574 g         1728 t
ORIGIN      
        1 gcgaaatcgg ccgtcaatac gcagagcggc gtcagccacg tccacatgag gatcgcctcg
       61 gtgttgaaca acgcgatgta gtcggtggcg gtaccgaaca ggatcagcag gccggcgatg
      121 atgacccagt tgagcatcac cgtgctcacc acctcaaggc tggtcaggtt gatcttggac
      181 gcgctgggga aggtcagcga gaagaggatg agcgacagga tgaggtaatg cggctcgagc
      241 ccgccgttct tgagataggc cacgctccac aaggtgagca cgatcaccag tgggtcgagc
      301 agggcctccg tcgtgtacag caggttgtcg cgcgcgatgg cgacgctcag ccccttgcgc
      361 tggatggggg cagacttata ccgattcatt tggctcgggc atcatgctga gtttggcgac
      421 ggcttgcgca cggttgagta catccagctt cttgaagatg cgctgcaggt ggttcttgac
      481 cgtgaaggcg ctgatatcga ggatcatgcc gatctcataa ttggtcttgc cttgcttgac
      541 ccagtccatg atctcgtgct cccggatcga caaggtgcca aagccctcgt ctatcgacgt
      601 cgttaccggt ttgggtggct cgacggcgat cggttcgatc tggcgcaggg tgcagtccac
      661 atagggcagc agggtttcga gcatggtgcg cgatgcctgc gacaggtcgt ccgaggcatt
      721 gagcaggatg tacaggcaat catggcgacc gcggctgtcc ttgatgccgt ggatcatgcc
      781 gcttttcatg cgggcgaaat cttcgcccag ctgatcgaaa ccgaactggt tctcgctgaa
      841 caggccatgc tgcatgcgca tgctgagtgg cgcgtcgccc tgttcgtgcc agtagttgaa
      901 gagacgtttc agcagcggta ccagcctggc gtgctgcacc tgctgggtac gcattcccgg
      961 cagcggcgag accacatcga aagagatcag gccgagggag aaatcgcccc aggccgcgat
     1021 catgatgtcg tgtggcagga acgcctgcac gttgccttgc gaccagagca tgaactcgaa
     1081 atgggtgcgc acgctgagcg actcctggat catgccgaag aattgcgtct tctggacttc
     1141 gtctagttca gtatgtagtg gcatagatag tatcttcaca gtagtagttg ttgatttata
     1201 ttgaatcggg cccgccgcag cattcggcag ctcctcggcc ctgtaccgct tgaagttgtc
     1261 atgcaccgtc cgttgccgga gcgtcttgat gacgttgcat cgatcacaac cgattcacgt
     1321 ctttcgtgcg ctagctagtg catttagcgt gccacttttc caactatatg atttatctat
     1381 gaaaacaccg gaatcgccga tttcggcgac ctgctgaagt gtcagcgatc cccgacatct
     1441 aatatttcat tcttgttaag tgaatgatct gtcgaccttt tttctgtctc tccaaacaga
     1501 cgctggcaga aaagtgcgat ttgggagcgc tcgcccggtc ttgatgcaag atctgccggg
     1561 catgcgtcgg ggcgtacgtg gatggccttg aaagccgcgt ggatactggg ttttggcctt
     1621 agtccgttcg gcctattgtg agggtggggc tgaaatcact aggatgcacg aaaccttcaa
     1681 gggggttgcc gtcgcaagca tcggccggtc gcgcctcttg agccaaccgt tgattgaggc
     1741 atccacgcat atgagccaga cattgatagc accttacagc gacatcgagc ccctgggccg
     1801 ggctgatcgc tatatcgacg agattatgga gatcgtgcat cagatcgatc ttttcgagtc
     1861 gctgagcgcg cccgagatcc tggcggtctg ccgccatatg gattgttatg ccgcgccgcg
     1921 cgggtacagc ctcttgcgac aggatgcgac cgacgaccat ctggtgctgc tgctcagcgg
     1981 tactgcggtc atcaagcgcg acagcttgct ggatggagag gagatcatcg ccgaagtcag
     2041 tgccggcacg ttgcttggcg agatgtcgat gatggacagc aaaccccatc tcaccagttg
     2101 cgtggcgatc actccgttgg atttcgccgt gttctcgcgc gatacgctga acaccatcct
     2161 ggtcaatacg ccgcggctcg gcaacaagtt gctgttgacc atgctgcatc tcgtgtctgc
     2221 gcggctgcgc gacgcgagct accgatttct gccaagcctg gcatgagcgt accgaaccct
     2281 cgtcgattca tgaaggagac tgacgtgatg atcaaatctt ttaccaaact gctgggcctg
     2341 ttcctgctcg tatgcatact ggcctacctg ccgtcctctt atgcggcttc caacgattac
     2401 cagctgggcg cgggcgacgc ggttcgcatc tccgtgttcc agaatccgga cttgtcgctc
     2461 gagacccggg tctccgaatc cggcagcatc tcctatcccc tgatcggcga agtgcaggtc
     2521 ggcggactct cgccgcgtga agccgagcag ctgatcgcga agaagctgat ggacggcaat
     2581 ttcgtcaagc ggccgcaggt caacctgacg ctggtcgagg tgcgcggcag ccagatcgcc
     2641 gtgatcggcc tcgtcaacaa gccgggccgc ttcccggtcg agagcgccca catgcggctg
     2701 tctgaagcca tcgccgccgc tggcggcgta tccgacgcag ccggtaccgg gacggctgtg
     2761 gtcaccggca agcgcgatgg tcggccgatg cgtgtcgaaa ccgatatcgc ctcactgttc
     2821 cttagcgacg gcggtggcca ggacaccgag ctcaaggccg gcgacatcat ctatgtcatg
     2881 accggcaacc aggtctccat ccaggggcag gtcaaccgtc ccggtcgcta tgccctggaa
     2941 agcaacaaga tgcatgtctc cgaagtgctg accctggcgg gcgggctgac gcctgacgcc
     3001 gccgatgtgg cgatcgtcgc cggcaagcgc aatggcgaga acttccgcaa ggagatcgac
     3061 atcccggcgc tatacctcag cgacagtaac gaccagaacc tgctgatcgc cgcgggcgac
     3121 gaggtctacg tgcagaaggc gccggtctat tacatctacg gcgaagcaca gaagcccggt
     3181 tcctatcgca tcgaacgcaa tatgaccgtg atccaggcgc tggcgcaagg cggcgggccg
     3241 accatccgcg gcacgcagcg caacctcaag ctcatgcgtc gtgacagtca cggcgaaacc
     3301 gtcgagctta cccccaagct caccgacctg gtgcgcccgg acgatgtcat ctacgtccgt
     3361 gaaagcctgt tctaaggagc tgtcatgtcg ctgaccaacc tgttcctgat cctcaaggcc
     3421 cgaatcaaga tcgtggtctt catcttcgtc gtcaccgtgc tgaccgcttt tgtggccagc
     3481 atgatgatgc cgaagtatta caaggcgagt acgcaggtcg tggtgaatta ccgcggcaac
     3541 gactcggtga ccggtaattc caaccagtcg ctcatgacct cgagttacct tgccacccag
     3601 gtcgacatca tcaagaacag gagcgtggcg ctgagcgtgg tcgatgcttt gcaactggtc
     3661 gacaacgatg ccatgaaaaa ggatttctac gaagtcacca agggccgcgg cgatatccgc
     3721 cagtggatcg ccggtcggct catcagccgg ctcgaggtct cgcccttgcg tgagagcagc
     3781 gtgatggaga tcaccttcag cggcaaggat ccgcaacaag tggcggagat cgccgatgcg
     3841 ttcgccgagg cctatctcga tcgtactcta cgactcaaga tcgagccggt gaaaaaggcc
     3901 gccaacttct tcaaccaaca aatgaccacg ctgcgcgata gcctggaaaa agcgcaatcg
     3961 aaactttcgc aataccagca gacccacggc atcaccagta ccgaccagcg tctcgatatc
     4021 gagacggcca agctcaacga gctttccagt cagctggtgg cggcgcaggc ttcggcgatc
     4081 gaagcgaatt cgcgctacgg caacagccgc aaggacgccg gtaactcgcc ggacgtggcg
     4141 cagaaccccg tgatccagaa cctgcgggtg gaagtggcgc gcgcgtcggc caaggtggcc
     4201 gacctgtcgg agcggctggg atccaaccat ccggagtatg aagccgccgc tgctgaactg
     4261 aagaacgtcc agagccagct ttatactgag atcgcgcgcg ccaacaacaa catcaagggc
     4321 tcggccatca tccagcagca gcgtgtcgaa gagctgcgct cggcgctcgc caagcagaaa
     4381 ttggcggtgc tggcgttgaa ccgccagcgt gacgagatgg cggtcctgca ggaagacgtc
     4441 gagacggcgc agaaggcgct tgacgccgtc acccagcgct tcacccagac cagcatcgaa
     4501 gcgcaatcga accagaacga tatcgccatc ctgtctgccg cgcaggtgcc gggctcaccc
     4561 tatacgccgc gcatcatgct caacatgctg ctcgccatgt tcgtcggtgg cgtgctcggg
     4621 ctgggcttcg ggcttatcgc cgagatgctc gatcgccggg tacgcagcag cgacgatatc
     4681 gctgagctgc tcaaggtgcc tgtggtggcg ctggtcaaca agcgaccagc ggtcaccggc
     4741 atgaaaatgc tgccgggtgc gcagtcgggc aaattcttcc catcaaccta ggatcaagat
     4801 gaatatcagc caactcaatt ccccgccgat cccgctcggc gcagagcgcg accagggccg
     4861 tcaggagtct gaacgcaagg caccgcttgg ccagttgctg ttgaagatgg gcaagctcac
     4921 cgaggacgag atcgaacgtg tgctcgcctt gcaacaacag accggcctgc gcttcggcgc
     4981 tgcggcgaag aagctcggcc tggtcagcga gaccgatatc cagcatgccc tggcggtgca
     5041 gttcgattat cactatctgc acaagagcca ggaatttttc agcaagcgcc tgatcgcggc
     5101 ctatgatccg ttctcgccgc aggtcgaagc cctgcgagcc ttgcgtagcc agttgctgct
     5161 gcgctggttc gacgatgcgg agaaagtgct ggccgtggtc gctgccaatc caggcgaggg
     5221 ctgcagcaac ctggccgcca acctggcggt ggtgttctca caactgggcg agcgtacctt
     5281 gctcatcgac gccaacctgc gcgagccgga tcagcgcaac atcttcaatc tctacgaatc
     5341 gcgcggcttg tcggacatcc tggccggacg cgccgacctt gacgtgatcc acaagatcga
     5401 ttcgctcagt ggcctgtcgg tgctcggcgc cggtacggtg ccgcccaatc cgcaggagtt
     5461 gctgagccga aaggtgttcg gcgaagtgct gcgccgttgc cgtgagcaat acgacgtcat
     5521 cctcgtcgac acctatcccg ccagcatgac ctccgatgcc cagaccgtcg ctgccagatg
     5581 cggcggcgcg ctgcttgtgt cccgccttaa ccagacgcgc atggcccagc ttgccaatgt
     5641 gcgcgaccag ctgttgatga gcgaagtcga gatcgtcgct gcgatcgtca atgacctgta
     5701 gatcacccgg caacaacaag gcgcaggata gaaatagatg acagcactgc tcaagtcgtc
     5761 cggcaccacg ctggtcacca ccatcgtgat caccgccatc ggtttcatca cgtcggtgat
     5821 caccgcccgc gtactcgggc cggaagggcg cggcctgctc tccggcgcgc tattgatcgc
     5881 gacgctggcc ggcaatgtcg cgctgttcgg cttggccaac agttatatct accacaaggg
     5941 caccggccgg ccgttccgtt acgggctgtt cttcgccggt tcgctcttgt tcgtggcagg
     6001 gctctcggcg ctgctcggct tgatcgggac gcaggtgatg acgcacgagg cgcaactgca
     6061 cgcgcagttg ccgctgatcc tgttgctcac cgtggtcacc gccacccagg gctatttcta
     6121 cgccctgagc cagctgcatc acggcctgcg gttcttcaat ctcatgcgtt tcctgctggt
     6181 gatcggcaac ctgatcctgc tgctcgggct gctgctgttc ttcaagcccg tcgatttccg
     6241 catgatcctc gtcacgcaga ccatcgtcag tgccggcctg acgctcgccg gcatcgcctg
     6301 ggcacgtcac cacaggatct gggagggtag cgacaccacc caagcgcccg tggcctggaa
     6361 gagcatgctg ggctatggcg cgagccatca cggcacggtg gtcatcggcc tgctgctggt
     6421 caatttcgac aaggtggcct tgctcaacat cggtaccatc gtcgaatacg gtttctatgc
     6481 gctggccttc accacctcgc gccttatcgg cgcattgcag gaggccgtct caactgcgct
     6541 gtattcgcgc catgccggtc gagacgtgca agtgctgtcc gagaatgtgc ggaccgcgtt
     6601 tcgcatgacg ttcgtgccca tgctggcagt ggcggccatc ggcgccttgc tctcgccctg
     6661 gctcatcgtc ctggtctatg gcgaatcatt cgcttccacc gtgatcccgt ttgcggtgct
     6721 gctgttcgaa tgcgtgatct cgggcgcgag ctggaccctg gcgcaacgct tcaatgccgg
     6781 cggtcgaccc gggctggtgt tcgtgcgcca gttcatctcg gtgctgccga tctttatcgc
     6841 cttgccgttc ctgccgcacg agaacatcca tgtctatctc agcctgctga tgctggcagg
     6901 cggcatcctg cggctgtcgg tgacgctggt catctatccg accgtgctca aggagcgcgt
     6961 gccggatatc ctgccgaccc tggccgattg ccgggcggtc ttcaatgtgc tggcacgtcg
     7021 ccaggtcgcg gatggaaaag catgaatcga accgactccc atcaggcgct gaagcatccc
     7081 gtggtcaacc atgccttgct gatgggcgca ttggctacgc aacacgatgt gctgctggac
     7141 ctcatcggca accgcccact gcattatgtc gatctgccgg tccacggcaa tatcggcgat
     7201 ctgctcatca tgcttggcac actggcattc tttcagcaca actacctggt gccgaaagtc
     7261 agcgccgcgg ctttcgccta tgagccgagc tggatcgctc cgggcgatgt ggtggtgttc
     7321 cacggcggcg gcaatttcgg cgacctctat gccgaatacg gcatgcaggg cctgcgtgag
     7381 caggtggccg atcgctatcg cgacaatcgc atcgtggtgt tgccgcagac cctgtatttc
     7441 tcctcgcagg cagccatgcg ccgttccgcc gcggtcttcc gccggcatcc ggacctgcac
     7501 ctgtgcgtgc gtgaccgcat ctcctacgac atcgcacgca atttcaccga caacgtctac
     7561 ctgctgccgg acatggcaca ccagctctac cgactcacgt cccgacacga tgcgccccat
     7621 ggcaccttgc gcatccagcg caccgatgac gaacgcgccg aagctggcgc aatgcctggc
     7681 ctcgagatcg tcaccaccac ggactggccg atggtcgtcg gcgacggcga gcgacggatc
     7741 gcgctgttcc gtcgcgggat gcgtgccctg cacaaatatg gcctcggctg gtacggcaac
     7801 aagttgctgg ccggcctctg ggcagactac gcgcgacatc tggtgcaaca ggcgacgacc
     7861 ttgttcgcgg cgcatgagca catcgtcacg gatcgcctcc acggccatat cctggcctgc
     7921 ctgatggaca agcccaacac cgtgctcgac aacagttacg gcaagaacgc cacctacgtc
     7981 aatgcctgga ccggcaacag cgagctggtg accttgcaag cagcgtctgc cgaaaagatt
     8041 tgaaccaggc gtaatgattt gaaccaagtg taaagggaat acccatggct acattcgacg
     8101 tattgctccc cgtgaagaac ggtatcgact atctgggcga agccatcgat agcatctgcc
     8161 agcagacctt tcgggactgg cgtctgctga ttctggacca cggatcgaac gatggcagcc
     8221 ttgaactcgc acaacgctat gccgggcagg atcctcgtgt cgaggtgcat tgcctgccct
     8281 cggcccaggg gctcgccggg ctgctcaacg ccgggctgga tctgtgccac ggcaagtacg
     8341 tgctgcgcca ggacgccgat gatatctcgc tgccgaaccg catgcaggtg ctgttccatg
     8401 cgctcgaaga ggacgacgaa ctggtgctcg tgggttcgct cggcgacgta gtggatgccg
     8461 atggtaacaa gatcggcatt ctcgacatgc cgaccggcca gttcggggtg ctcatcagca
     8521 cgccgttccg cacgcctgtc gctcacccga cggtggccat gcgactcgac gccatccgtg
     8581 gcctcaacgc gcgctatggg gtcgatt
//
LOCUS       DATMTW010000256         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_256892, whole genome shotgun sequence.
ACCESSION   DATMTW010000256 DATMTW010000000
VERSION     DATMTW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256892"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /gene="glyA"
                     /locus_tag="VN084_05060"
     CDS             complement(<1..414)
                     /gene="glyA"
                     /locus_tag="VN084_05060"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749062.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWT28858.1"
                     /translation="MFSTAKTLSVVDPELWQHVESERHRQDEHIELIASENYTSPAVM
                     QAQGSQLTNKYAEGYPGKRFYGGCEFVDQIEQIAIDRLKKLYGAEYANVQPHSGSQAN
                     QAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGK"
     gene            830..1024
                     /gene="iscX"
                     /locus_tag="VN084_05065"
     CDS             830..1024
                     /gene="iscX"
                     /locus_tag="VN084_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HWT28859.1"
                     /translation="MKWKDIQAIAEALYDKHPDVDPKTIRFVDLHNHVVALEGFDDDH
                     NRGGEKVLEAIQQAWIDEAD"
     gene            1031..1996
                     /locus_tag="VN084_05070"
     CDS             1031..1996
                     /locus_tag="VN084_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWT28860.1"
                     /translation="MLAPYRPPCWLRGRHLQTIYPAAWMPLPEITYRRETWETPDGDQ
                     IALDWLAADTDAAAPLVVLFHGLEGDSRSHYALRLMQGVQARRWRGVVVHFRGCGGLP
                     NRLPRGYHCGDSNEIDWILKRLKTMNDTDLYAVGVSLGGNAMLKWLGEQGHAACAVIE
                     RAVAISAPLALPTTGHVLGSGFNKLYARLFLKTLRPKALAMIERHGLPFDREAIAGVD
                     NLWHYDGLFTAPLHGFKDADDYWAQSNSRPHLKSIAVPTLLINARNDPFLPEKDLPQP
                     HEIADSVSCEFPAEGGHVGFASKRRQPDWLAARVLCFLQSQPQSA"
     gene            2007..3413
                     /locus_tag="VN084_05075"
     CDS             2007..3413
                     /locus_tag="VN084_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWT28861.1"
                     /translation="MQAPKEIFKAYDIRGIVGKTLTPEIVEAIGQAIGSKAVDRGQTH
                     ICIGYDGRLSGPSLAAALSRGIRKSGIHVINLGMVATPMVYFSAHHLDTHCGVMVTGS
                     HNPPDYNGLKMVLAGETLSGETIQDLRQRIEQGRFHHGEGHESSHDIAPAYIARIAAD
                     IKLARPMRIAVDCGNGVPGAFAAKLYSAIGCEVKELFCEVDGHFPNHHPDPSVPENLK
                     DLIQTLKTDDAELGLAFDGDGDRLGVVTKDGQIIYPDRQLLLFAEDVLKRAPGATIIY
                     DVKSTRNLAPWIKARGGQPLMWKTGHSLVKAKMKQTGSALAGEMSGHVFFNDSYLDEA
                     SQRVRRWYGFDDGLYAGARLLEILSRSADPSAILNALPDSTSTPEQHIQMREGEPHAL
                     IAQLQQSAVFPGANEVITVDGLRVEYPDGFGLMRPSNTTPVVVLRFEADNEPALKRIQ
                     AQFRDVILAAAPHVSLPF"
     gene            3434..3910
                     /locus_tag="VN084_05080"
     CDS             3434..3910
                     /locus_tag="VN084_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWT28862.1"
                     /translation="MSRNTLIVIAIWLGLAYGLYYLAEGMLHPNKVEVLGEDRTVTLQ
                     RGPDGHYRAEALINGQPVNVLVDTGATGVAISQRVADKLGISSRTAVRTNTANGDSVA
                     YMTRLRSVRLGGVEAHDVSALIAPGLDGDVLLGMSYLGRMDVRLYRGTMTIKQLDE"
     gene            4104..4577
                     /locus_tag="VN084_05085"
     CDS             4104..4577
                     /locus_tag="VN084_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWT28863.1"
                     /translation="MTTPYHTLKALSVALITTFAFTQAQAAMLSTGGYNTELHKMEMM
                     HMLDGNADHMVTEQEFRTYYGEVFNEIDTNHDGALDAKEWVGTGGKQEIRLATGGYSQ
                     ELRKMEMMKSIDTNGDHKVTKGEFVTFHQALFTGMDANHDKQVDPQEWLAKQTGQ"
BASE COUNT          986 a         1499 c         1342 g          874 t
ORIGIN      
        1 cttgccggac aggttggcag gagaaccatg ggtgaggtga ccgccgtgac cgagattcat
       61 gcccatgacg gtatcgcccg gcttgaggat ggagaaatac accgcctggt tggcctgcga
      121 accggagtgc ggttgtacgt tagcgtattc ggcaccgtac agtttcttca ggcggtcgat
      181 ggcgatctgc tcgatctggt cgacgaattc acaaccgcca tagaagcgct tgcccggata
      241 accctcggcg tacttgttgg tcagctgcga accttgcgcc tgcatcactg cagggctggt
      301 gtagttctcg gaggcgatca gttcgatgtg ctcgtcctga cggtgacgtt cgctctcgac
      361 atgctgccag agttccgggt cgacgacgct aagggttttt gctgtgctaa acatcttatg
      421 tatatccagg agccgaaaaa taatgtgatt ttaccatgtt cgggtggttc gagcgtctgc
      481 agcacatgca agttcacaca ggtcggctac ggccgccgcg ccaccctcgc cgcccccagg
      541 atggatggct gtaaataacc aggtggctgg tgcctaccac cggccaaacc tgaagccccc
      601 gcgcaggcga ttgattctta tcggataaat ttcaggcacg cagcttgcgt actcccatgt
      661 cgaagcgctt gtctccaatc gctctccttc accccgccac ccatcaatcc caggtggcgg
      721 gatttttttt gccgctcgcc catgggccga ccgcgctata atttccgcaa gctgcatttc
      781 gtctatgggc aaaacgccca gcacccggca agaccagagg atatcccaca tgaaatggaa
      841 agacatacaa gccatcgccg aagcacttta cgacaaacac ccggacgtcg acccgaagac
      901 catccgcttt gtcgacctgc ataaccatgt cgtcgcgctc gaaggcttcg acgacgacca
      961 caaccgcggc ggcgagaaag tgctggaagc gatccagcag gcctggatcg acgaggctga
     1021 ctgacggggc atgctggcgc cttatcgccc accttgctgg ttgcgcggcc ggcatctgca
     1081 gaccatttac ccggcggcct ggatgccctt gccggagatc acctaccggc gagagacctg
     1141 ggagaccccg gacggcgacc agatcgcgct cgactggctg gcggcggaca ctgacgctgc
     1201 agcgccgctg gtcgtactgt tccatggctt ggagggcgat tcacgctcgc attacgcatt
     1261 acgcctcatg cagggcgtgc aagcgcggcg ctggcgtggc gtggtcgttc atttccgcgg
     1321 atgtggtggc ctgcccaacc ggttgccgcg cggctaccat tgcggtgatt caaacgagat
     1381 cgactggatc ctgaagcgct tgaagaccat gaacgacacc gatctgtatg cggtcggcgt
     1441 ctcgctcggc ggcaacgcca tgctcaaatg gctgggcgaa caaggccacg cggcttgtgc
     1501 cgtcatcgag cgtgccgtcg ccatttccgc cccgctagcc ctacccacca ccgggcatgt
     1561 gctcggcagc ggcttcaaca aactctatgc gcggcttttc ctcaagacct tgcggcccaa
     1621 ggcgctggcc atgatcgaac gtcatggctt gccattcgat cgcgaagcca tcgccggcgt
     1681 cgacaacctg tggcattacg atggcctgtt caccgcgccg ctacatgggt tcaaggatgc
     1741 cgacgattac tgggcgcaat ccaacagccg cccacacctg aagtcgatcg cagtgccgac
     1801 cctgctcatc aacgcacgca acgacccttt cctgccggag aaagacctgc cgcagccgca
     1861 cgaaatcgcc gacagcgtca gttgcgagtt cccggccgaa ggtggtcatg tcggctttgc
     1921 cagcaaaaga agacagccgg actggctagc ggcacgtgtc ctttgtttcc tgcaatcaca
     1981 acctcaatca gcgtagacat cacaccatgc aagcacctaa agagatattc aaggcctatg
     2041 acatccgcgg tatcgtcggc aagaccctga cccccgagat cgtcgaggcc atcggccagg
     2101 ccatcggcag caaagcggtg gacaggggcc agacccatat ctgcatcggc tacgatggcc
     2161 gcctctcggg tcccagcctc gccgctgcgt tgtcgcgggg catccgcaag agcggcatcc
     2221 atgtcatcaa cctcggcatg gtcgccacgc ccatggtcta cttctctgcc caccatctcg
     2281 acacgcactg cggcgtgatg gtgaccggca gccacaaccc gccggattac aatggcctga
     2341 agatggtcct cgctggcgag accttgtccg gggagacgat ccaggacttg cggcagcgca
     2401 tcgagcaagg tcggttccac cacggcgaag gccacgaaag cagccacgac atcgcgccgg
     2461 cctacatcgc gcgcatcgcc gccgacatca agctggcccg ccccatgcgc atcgccgtcg
     2521 attgcggcaa cggcgtgccc ggcgcattcg ccgccaagct gtattccgcg atcggctgcg
     2581 aagtgaagga gttgttctgc gaggtcgacg ggcatttccc caaccatcac ccggacccgt
     2641 cggtgccgga gaatctcaag gatctgatcc agaccctgaa gacggatgac gccgaactcg
     2701 gcctggcttt cgacggcgac ggcgaccggc tcggcgtcgt caccaaggac ggccagatca
     2761 tctaccccga ccgccagcta ttgctgttcg ctgaagatgt gctgaaacgc gcacctggcg
     2821 ccaccatcat ctatgacgtc aaatccaccc gcaaccttgc gccctggatc aaggcgcgcg
     2881 gcggccagcc gttgatgtgg aagactggcc attcgctggt caaggccaag atgaagcaaa
     2941 ccggctcggc gctggcgggc gagatgagcg ggcatgtgtt cttcaacgat tcctacctgg
     3001 acgaggcgtc gcaacgcgtg cgacgctggt acgggttcga cgatggcctt tacgccggtg
     3061 cgcgcctgct ggagatcctc agtcgcagcg ctgacccgag cgccatcttg aatgccttgc
     3121 cggacagcac cagcacaccg gagcagcata tccagatgcg cgaaggcgag ccgcacgccc
     3181 tcatcgcaca actgcagcag agcgctgtct ttcctggcgc gaacgaagtg atcacggtcg
     3241 acggcttgcg cgtcgaatat ccggacggct tcggcctcat gcggccatcg aatacgaccc
     3301 ccgtcgtcgt gctgcgtttc gaggccgaca acgagccagc cctgaaacgt atccaggccc
     3361 agttccgcga tgtcatcctc gcggcggcgc cgcacgtgag cctgccgttc taggcctgaa
     3421 gaaagctccc agcttgtcac gcaacacgct catcgtcatc gcaatctggc ttggactggc
     3481 ctacggactc tattacctgg cggaaggcat gctgcacccg aacaaggtcg aggtgctggg
     3541 cgaagaccgc accgtgacgc tgcaacgcgg accggatggc cattaccgcg cggaagccct
     3601 gatcaacggc cagccagtca acgtactcgt cgataccggc gccacagggg tcgccatctc
     3661 tcagcgcgtc gcggacaagc tgggtatcag cagccgcacc gccgtgcgca ccaacacggc
     3721 caacggcgac agcgtagcct acatgacgcg cttacgctcg gtgagactgg gcggcgtcga
     3781 agcccatgat gtctctgcgc tcatcgcccc cgggctggat ggcgacgtct tgctcggcat
     3841 gtcctacctt ggcaggatgg acgttcgtct gtaccgtggc acgatgacca tcaaacagct
     3901 cgacgaatag ccagttagcc tttgcccgta tttgcatcgc ccgcatttgc acttgccgaa
     3961 tcgatcctgg cgcagcatgg aaactgtcca cggccacacc gcgataatat ttttgtaagt
     4021 aatcgcaccg catcgtgact aacggtcact aaatgcattt agtcatgttt tccttaacaa
     4081 cctatcctta tcaaggagtt accatgacga ccccctatca cacactcaag gcgctttcag
     4141 tggcgctgat taccaccttc gcattcaccc aggcacaagc ggccatgctc agtaccggcg
     4201 gctacaacac cgagctacac aaaatggaga tgatgcacat gctggacggc aatgccgacc
     4261 acatggtgac cgagcaggaa ttccgcacct actatggtga ggtattcaac gagatcgaca
     4321 ccaaccacga cggcgcgctg gatgccaagg aatgggtagg caccggcggc aagcaggaaa
     4381 tcaggctggc gaccggcggc tacagtcagg agctgcgcaa gatggaaatg atgaagtcga
     4441 tcgataccaa tggtgatcac aaagtcacca aaggcgaatt cgtgactttc caccaggctc
     4501 tgttcacggg catggacgcc aaccacgaca agcaggtcga cccgcaggaa tggctggcga
     4561 aacagaccgg ccagtagcac gattgttctg caccgaaagc cgcgtctgtg gcgattgatg
     4621 caacaaaaaa aggccagcgc aagctggcct ttttctatgt ctgcgcgact tttcccggca
     4681 gcccctgcag cagcaccagt t
//
LOCUS       DATMTW010000257         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_626965, whole genome shotgun sequence.
ACCESSION   DATMTW010000257 DATMTW010000000
VERSION     DATMTW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626965"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VN084_05090"
     CDS             complement(<1..397)
                     /locus_tag="VN084_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWT28864.1"
                     /translation="MSLKARITEDMKSAMRAKDSARLGAVRLLQAAIKQREVDERIEL
                     DDTQVVEAIEKMLKQRRDSISQYEAANRHDLADVEKYEVSVLQEYLPQALTEDEVDAL
                     LAKVIADTAAAGIKDMGKVMAAIKPLVVGR"
     gene            complement(423..635)
                     /gene="rpsU"
                     /locus_tag="VN084_05095"
     CDS             complement(423..635)
                     /gene="rpsU"
                     /locus_tag="VN084_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWT28865.1"
                     /translation="MSTVRVKENEPFDVALRRFKRMIEKTGLLTDLRAREFYEKPTWE
                     RKRKAAAAVKRHYRRIRNQTLPAKLY"
     gene            736..1749
                     /gene="tsaD"
                     /locus_tag="VN084_05100"
     CDS             736..1749
                     /gene="tsaD"
                     /locus_tag="VN084_05100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWT28866.1"
                     /translation="MLVLGIESSCDETGIALYDTERGLLSHALHSQIDMHAEYGGVVP
                     ELASRDHIRRALPLTRHVLDIAGRTLQDIDGIAYTQGPGLAGALLVGTSIAEALAFTL
                     DVPTVGVHHLEGHLLAPLLEENPPAFPFVALLVSGGHTQLMRVTAIGDYELLGDTLDD
                     AAGEAFDKTAKLLGLGYPGGPALSRLATQGQAGRFKLPRPLLNSGDLNFSFSGLKTAV
                     LTLVNEHALTDQTKADIACEFQEAVTEVLTAKCMAALRETGLDSLIVSGGVGANARLR
                     ERLNQATQRRHCTVSYPRLEFCTDNGAMIAFAGAMRMLHAQRGDHAFTVRPRWDLADL
                     QAP"
     gene            complement(1781..2398)
                     /gene="plsY"
                     /locus_tag="VN084_05105"
     CDS             complement(1781..2398)
                     /gene="plsY"
                     /locus_tag="VN084_05105"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWT28867.1"
                     /translation="MNELGFIPVVFIPIIWIVAAYLLGSISFGILISRMFGLPDPRTV
                     GSGNPGATNVLRSGKKMAALLTLLGDAFKGWFPVWLALQSGMLMWVVSWVGFAVFLGH
                     LYPIYYRFKGGKGVATALGVLLALSVWLGLAVVIVWVVVFAIWRYSSLAAIVAALLAP
                     VIAYFLLPYKDYLLLTLVITVLLIWRHRSNIQKLLAGTEAGFGKK"
     gene            2458..2814
                     /gene="folB"
                     /locus_tag="VN084_05110"
     CDS             2458..2814
                     /gene="folB"
                     /locus_tag="VN084_05110"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWT28868.1"
                     /translation="MDTIFLKEVKVETRLGVPDWERLSPQTILLDIELAMPHSRSCET
                     DAIEDTIDYGVIVQRIRQTLAEHTFKLVEALAEHLCQLILNEFGTPWVKIKVSKPGIL
                     PGVHSLGVVIERAKAD"
     gene            complement(2827..>3480)
                     /locus_tag="VN084_05115"
     CDS             complement(2827..>3480)
                     /locus_tag="VN084_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWT28869.1"
                     /translation="RFFRYCMREGNVSADPTEQIDSPKLPRALPKSLNEEEVEALLKA
                     PAIDEPLGLRDRAMLEILYAGGLRVSELVGLKVTEVSLSEGVIRVTGKGSKTRLVPMG
                     ELAVEWTARYLKESRPEILQKRLSDSLFVTQRGSAMTRQAFWYLIKRYALLAGIHKPL
                     SPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVARERLKQLHSLHHPRG"
BASE COUNT          742 a         1075 c          961 g          702 t
ORIGIN      
        1 ctcgcccaac gaccagcggc ttgatcgccg ccatgacctt gcccatatcc ttgatgccag
       61 ctgccgcggt atcggcgatc actttcgcca gcaatgcgtc gacttcgtct tcggtcaggg
      121 cctgtggcaa gtattcctgc aacacgctga cctcgtattt ctcgacatcc gccagatcat
      181 gccgattggc cgcttcgtat tgcgaaatcg agtcacggcg ctgcttaagc atcttttcga
      241 tggcctcgac gacctgtgtg tcgtcgagtt cgatacgctc atccacttca cgctgcttga
      301 tggcagcctg caacaagcgc accgccccta aacgcgcgct atctttcgcg cgcatggcgg
      361 acttcatgtc ttcggtgatg cgggctttca gtgacacagc cggtgctccg tactgctaac
      421 gcttagtaga gcttggctgg cagggtctga ttgcggatac gacgatagtg acgcttaacg
      481 gcagcagcag ccttgcgctt gcgctcccag gtcggcttct cgtagaactc acgagcacgc
      541 agatcggtca gcagaccggt cttttcgatc atgcgcttga agcgacggag tgcgacgtca
      601 aacggctcat tttcttttac acgtacggta gacattaggc tcacttcctt gagaaggtgc
      661 gaaaaagttt gctattctag cgagctatgc atgatttatc aatgattaac tcttaatttt
      721 gccctctgac ccagcatgtt agtacttggt atcgaatcct cttgcgacga gaccggcatc
      781 gcgctttatg acaccgaacg cgggctcctg tcgcacgcct tgcattccca gatcgacatg
      841 catgccgaat atggtggcgt cgtgcctgaa ctggcctcgc gcgaccatat ccgtcgcgcc
      901 ctgccgctga cccgacacgt gctcgacata gccgggcgaa cgttacagga catcgacggc
      961 atcgcctata cacagggtcc cgggctggcg ggagccttgc tcgtcggcac cagcatcgcc
     1021 gaagccctgg cgttcacgct cgacgtacca accgtcggtg tacaccatct tgaaggccac
     1081 ctgctcgctc ccctgctgga agagaatccg ccggcgttcc cattcgtcgc cttgctggtc
     1141 tccggcggcc acacccagct catgcgggtc acggccatcg gcgattacga attactcggc
     1201 gatacgctgg atgacgcggc gggagaagcg ttcgacaaga ccgccaagct actcggactg
     1261 ggttatccgg gcggacctgc gctttccagg ctcgctacgc agggtcaagc cggtcgtttc
     1321 aaattgccac ggccgctact caatagtggc gatctcaatt tcagcttcag cggcttgaag
     1381 acggccgtgt tgacgctggt taacgaacac gcgctgacag atcagaccaa ggcggacatc
     1441 gcttgcgaat tccaggaagc ggtgaccgag gtcttgacag cgaaatgcat ggccgctctg
     1501 cgtgagaccg ggctcgatag cctgatcgtc tcaggcggcg tcggggccaa cgcacggctg
     1561 cgcgaacgac tgaatcaggc gacacaacgc cgacactgca cggtgagcta cccgcgtctg
     1621 gaattctgca cggacaacgg cgccatgatc gcttttgccg gcgccatgcg catgctgcac
     1681 gcacaacgcg gcgatcacgc tttcaccgtc aggccgcgct gggatctggc agacctccaa
     1741 gcaccctgat aaagcgccct gagacagccc gcctgaaggc ctacttcttg ccgaaaccgg
     1801 cttcggtgcc agccagcagt ttctggatgt tactgcgatg ccgccagatc aaaaggaccg
     1861 tgatcaccag tgtcagcagc aggtaatcct tgtagggcag caggaaatag gcgatgactg
     1921 gcgctagcaa cgctgccacg atggcagcaa gggatgaata gcgccagatc gcgaacacca
     1981 ccacccacac aatgaccacc gccagcccca gccaaaccga cagggccaac agcacaccga
     2041 gcgcagtggc gacgcccttg ccacccttga accgataata gatcggatac aaatggccaa
     2101 ggaacaccgc aaaaccgacc caactgacta cccacatcaa cataccggac tgtagcgcca
     2161 gccagaccgg gaaccatccc ttgaacgcat cgcccagcag ggtcagcaac gctgccatct
     2221 tcttgccgct acgcaagaca ttggtcgcgc ctggattacc cgaaccgact gtgcggggat
     2281 ccggtaatcc gaacatgcgg ctgatgagga taccgaacga gatcgaaccc aatagataag
     2341 ccgccacgat ccagatgatc gggatgaaga caacgggaat aaaacctaac tcattcatgg
     2401 gagatgctct agaattctct gagtacgaat tctaaaccat gtggactgaa ttgttagatg
     2461 gataccatat tcctgaaaga ggtgaaggtc gagacccgct tgggtgtccc agactgggag
     2521 cggctatcgc cgcagaccat tctcctcgat atcgagcttg ccatgccgca cagccgctca
     2581 tgcgagaccg atgcgatcga ggatacgatc gattatgggg tcatcgtcca gcgcatccgc
     2641 cagactctgg ctgaacatac tttcaaactc gtcgaggcgc tcgccgagca tctctgccaa
     2701 ctcattctca acgaattcgg cacgccttgg gtgaagatca aagtctccaa gcccggcatc
     2761 ctgcctggtg tccattcgct cggggtcgtc atcgagcgcg ccaaggctga ctgagttcct
     2821 cttcggctag ccacgcggat gatgcaggct atgcaactgc ttcaggcgct cgcgcgcgac
     2881 atgggtatag atctgtgtgg tcgagatatc ggcatgcccc agcagcatct gtactacccg
     2941 caggtcggcg ccatggttga gcagatgcgt tgcgaacgcg tgccgcagcg tatgcggcga
     3001 aagcggcttg tggattccgg ctaacagtgc gtagcgcttg atcagatacc agaatgcctg
     3061 gcgcgtcatg gcactgccgc gctgggtcac gaacagactg tcgctcaaac gcttttgcag
     3121 gatctcagga cgcgattcct tcaggtaccg ggcggtccac tccaccgcca gttcgcccat
     3181 cggcacgagc ctggtcttgc tgcccttgcc ggtgacgcgg atcacgcctt cgctcaagct
     3241 cacctcggtg accttcaacc ctaccagttc ggagacgcga aggccgccag cgtagaggat
     3301 ctccagcatg gcgcgatcac gcaaacccag cggttcatcg atcgcaggtg ccttgagcag
     3361 ggcttcgacc tcttcttcgt tgaggctttt gggcagcgcc cgcggcagct tgggggaatc
     3421 gatctgctca gtcggatcag cgctgacgtt gccttcacgc atacagtagc gaaagaacct
//
LOCUS       DATMTW010000258         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_155980, whole genome shotgun sequence.
ACCESSION   DATMTW010000258 DATMTW010000000
VERSION     DATMTW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155980"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VN084_05120"
     CDS             <1..328
                     /locus_tag="VN084_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28870.1"
                     /translation="NPAEISEIPTTEKEFVAVINSVDKNKIIEQFGEPSKKDDVTHFK
                     SGKVIASIWHYHFINTDENGTYYETTELDFVDDKVVMVVFMNNNGEDPNADAGKAIEV
                     PVKPEL"
     gene            complement(432..851)
                     /gene="mscL"
                     /locus_tag="VN084_05125"
     CDS             complement(432..851)
                     /gene="mscL"
                     /locus_tag="VN084_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWT28871.1"
                     /translation="MFKEFKAFAMRGNVVDMAVGIIIGAAFGAIVKSLVDDVIMPPIG
                     LLLGNVDFSNLFLVLKEGATAAPYASVAAAKAAGAVTLNYGLFVNAIVSFTIVAFAVF
                     LLIRTINRLKTEEPSPPPAATPEDVLLLREIRDALKK"
     gene            968..2419
                     /gene="lnt"
                     /locus_tag="VN084_05130"
     CDS             968..2419
                     /gene="lnt"
                     /locus_tag="VN084_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWT28872.1"
                     /translation="MTTALLLGALAVTGFAPFYFYPSTIAALAGLCYLWQQAPTARAA
                     VWLGLSFGLGLFGAGIYWIYISLHIFGGMPPLGAAFATFCLCLFMALFPAAVGWLAKR
                     SGMLLIATPLLWALSEWVRSWIFTGFPWLTVGYTQVPYGPFAGFAPVVGVYGVSLVTV
                     LFAALIAALAIQWPHKPLRRSLVLSLILLWVSGSLLKHVEWSQPTGAPISIALLQGNI
                     TQDLKWQEDELQRTMDKYLAMVEDTQAKLVILPETALPVLAQDIPGDYLLALQQRAKA
                     NQGDLLLGAVEFEHGHFYNSMLSLGVSPMQAYRKSHLVPFGEFIPLKRVFGWIYRDWL
                     NIPLTDLSRGGFDQKPLTLAGQKVALNICYEDVFGEEIIRQLPQATLLVNTSNDAWYG
                     ESLAAYQHMQMSQTRALETGRMMLRATNTGATAIIDRHGEILAHAPHFSTQTLEGEAQ
                     GYTGSTPYVRLGNWPFILFSLAMLALLWGRKKK"
     gene            2464..>2785
                     /locus_tag="VN084_05135"
     CDS             2464..>2785
                     /locus_tag="VN084_05135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWT28873.1"
                     /translation="MALTFQQIILTLQTYWDKQGCALLQPYDMEVGAGTSHTATFLRS
                     LGPEPWKAAYVQPSHRPKDGRYGENPNRLQHYYQYQVVLKPAPENILELYLGSLEALG
                     FDLKK"
BASE COUNT          585 a          891 c          769 g          540 t
ORIGIN      
        1 gaatccggcc gagatcagcg agatcccgac cactgagaaa gagttcgtcg ccgtcatcaa
       61 cagcgtcgac aagaacaaga tcatcgagca gttcggtgag cccagcaaaa aggatgacgt
      121 cacccatttc aagagcggca aggtcatcgc ttccatctgg cattaccatt tcatcaacac
      181 cgatgagaac ggcacctact atgagaccac cgaactcgat ttcgtcgacg acaaggtcgt
      241 catggtggtg ttcatgaaca acaacggtga agaccccaac gccgacgccg gcaaggcgat
      301 cgaggtgccg gtcaagcccg agctctgatc tgcactgcaa cgatcgacac tgaaacgatc
      361 gaacccataa cgaaaaaggg cgcctagcgc ccttttttat cgtccggcgt ttctgccggc
      421 cagaacagcg atcatttctt caacgcgtcg cggatctcgc gtaacagcag aacgtcttcc
      481 ggcgtcgccg ctggcggagg agaaggctct tcggtcttca ggcggttgat ggtacggatg
      541 agcaggaaca cggcgaaagc cacgatggtg aaactgacga tggcgttgac gaacaggccg
      601 tagttcaggg tcaccgcacc ggctgccttg gctgccgcga ccgaggcata cggcgcggcg
      661 gtggcgcctt ccttcaacac gaggaacagg ttgctgaaat cgacattgcc cagcagcaag
      721 ccgatcggcg gcatgatcac gtcatccacc agcgatttga cgatggcgcc gaaagcggca
      781 ccgatgatga taccgacggc catgtcgacg acattaccgc gcatggcgaa ggctttgaat
      841 tctttaaaca tgatgttctc tcccggtggt gaattggtgg ctagccgcta taatgctgag
      901 caacctcgaa tcaagtcaac cttcaggatc gcccttgccc aagtttccca ccggtgaatc
      961 ggcccggatg accacggcgc ttttgctcgg cgcccttgcc gtcaccggtt tcgcaccctt
     1021 ctatttctat ccatcgacca tcgccgcact ggctggcttg tgctatctgt ggcagcaggc
     1081 gccgaccgcc agggcagcgg tctggcttgg actcagcttc ggcctggggt tgttcggggc
     1141 cggcatctac tggatctaca tcagcctgca tatcttcggc ggcatgcccc cgctcggcgc
     1201 cgccttcgcc accttttgcc tgtgcctgtt catggccttg ttccctgcgg ccgtcggctg
     1261 gctggccaaa cgctccggca tgctgctgat cgccacaccc ttattgtggg cgctctcgga
     1321 atgggtgcgc agctggatat tcaccggctt tccctggctc accgtcggct acacgcaagt
     1381 gccgtatggc ccgttcgcag gcttcgcgcc agtggtcggt gtctatggcg tgtcactggt
     1441 caccgtgctc ttcgccgccc tgatcgctgc actagcgatt caatggccac acaaacccct
     1501 gcggcgcagc ctggtcttga gcctgatcct gctgtgggtc agcggcagcc tgttgaagca
     1561 tgtcgaatgg agccagccga caggtgcgcc gatctcgatc gcgctactgc aaggcaacat
     1621 cacgcaagac ctcaaatggc aggaagatga actgcagcgc accatggaca aatacctggc
     1681 catggtggaa gacacccagg ccaagctggt gatcctgccg gagaccgcgc tacccgtgct
     1741 ggcgcaggat atccccggcg attacctgct ggcgctgcaa cagcgcgcca aggcgaacca
     1801 gggcgacctt ctgctaggcg cggtcgagtt cgagcacggc cacttctaca acagcatgct
     1861 cagccttggc gtctcgccga tgcaggccta ccgcaaatcg cacctggtgc cgttcggcga
     1921 attcatcccg ctcaagcgcg tattcggctg gatctatcgc gactggctca atatcccgct
     1981 gaccgatctt tcacgtggcg gcttcgacca gaaaccgctg acgctagccg gccaaaaagt
     2041 ggccctcaac atctgctacg aggatgtctt cggcgaggag atcatccgcc agctgccgca
     2101 agccacgtta ctggtgaaca ccagcaatga cgcctggtat ggcgaatcgc tggcggccta
     2161 ccagcacatg cagatgtcgc agacgcgtgc gctggaaacc ggccgcatga tgctgcgcgc
     2221 caccaacacc ggcgccaccg ccatcatcga ccgtcacggc gagatcctcg cccatgcgcc
     2281 gcatttcagc acccagaccc tcgaaggcga agcgcaaggc tataccggca gtacgcctta
     2341 tgtaagactc ggcaactggc cgttcatcct tttctcgctg gccatgctcg cccttttgtg
     2401 gggtcgcaaa aagaagtaaa atcaagccct ttcgccacat caccataaag cgccacgaca
     2461 aaaatggctt tgaccttcca acagatcatc ctcaccttgc aaacctactg ggacaagcag
     2521 ggttgtgccc tactccagcc ttatgacatg gaagtcggtg cgggcacctc gcacaccgcc
     2581 accttcctgc gttctctcgg cccggaaccc tggaaagccg cctacgtgca gcccagccac
     2641 cgtccgaaag atggccgcta cggcgagaac ccgaaccgtt tgcagcatta ttaccagtac
     2701 caggtggtgc tgaagcccgc gccggagaac atcctcgagc tgtatctcgg ctcgctcgaa
     2761 gcgctgggct tcgacctcaa gaaga
//
LOCUS       DATMTW010000259         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_391496, whole genome shotgun sequence.
ACCESSION   DATMTW010000259 DATMTW010000000
VERSION     DATMTW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391496"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VN084_05140"
     CDS             complement(<1..324)
                     /locus_tag="VN084_05140"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWT28874.1"
                     /translation="MRPSHYIFRVGAMCASLLLAFPAPAAESASDKPVVEQIVDVMTT
                     LAQGPHKGFRANHAKGIVATGTFTPASTAASLSKAAHLQQAETPVTVRFSNATGVPDI
                     PDTNPN"
     gene            323..508
                     /locus_tag="VN084_05145"
     CDS             323..508
                     /locus_tag="VN084_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28875.1"
                     /translation="MMFLDRLKEISESKTVLVASFCPGPTIVNHQYDKSSASRDMPTS
                     DWPYSRNPRKIKAFVLT"
     gene            531..737
                     /gene="thiS"
                     /locus_tag="VN084_05150"
     CDS             531..737
                     /gene="thiS"
                     /locus_tag="VN084_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWT28876.1"
                     /translation="MIQLIINGNPRQFDTSRLNVAELVDKLGLTGKRLAIECNGEIVP
                     RSQFAESPLHDGDKLEIVGAVGGG"
     gene            741..1520
                     /locus_tag="VN084_05155"
     CDS             741..1520
                     /locus_tag="VN084_05155"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWT28877.1"
                     /translation="MHTLTIAGNTYQSRLLVGTGKYKDFAETRAAIDASGAEIVTVAI
                     RRTNIGQNAGEPSLLDYLPPDEFTYLPNTAGCYTTDDAVRTLRLARELLDGHKLVKLE
                     VLGDPNTLYPNVVETLRAAEILVKDGFDVMVYTSDDPIIAKQLEEIGCCAVMPLASLI
                     GSGMGILNPWNLQIIIENAKIPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDP
                     VLMASAMRKAIEAGRESFLAGRMAKKLYSATPSSPTTGLIS"
     gene            1531..1914
                     /gene="apaG"
                     /locus_tag="VN084_05160"
     CDS             1531..1914
                     /gene="apaG"
                     /locus_tag="VN084_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWT28878.1"
                     /translation="MPTHKRYEFSVAVETAFVSDQSDIDQNRYVFSYSITITNTGTVP
                     AQLISRHWVITDAAGTVQEVRGLGVVGVQPLLKPGEQFEYSSGTVINTPVGTMHGSYH
                     MTAEDGTQFDTEIAPFTLSMPRVLH"
     gene            1935..>2496
                     /locus_tag="VN084_05165"
     CDS             1935..>2496
                     /locus_tag="VN084_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HWT28879.1"
                     /translation="MRALCLSGALLLSACGGEKAIKPEAASEPAKTVTRETPCHCPKP
                     VEYPPIYLPDTQKPAISGKPEVPSGPDYSMLKPGRWEDLEGIGEDDLSQAWAAWQQSC
                     STLRNKQPWQAACEAASAVDSHDQAAIQQYFRTYFSVYQTTNADGSDTGLITGYYEPL
                     LHGSRVRTERYRYPLYGRPDDLITVDL"
BASE COUNT          539 a          753 c          728 g          476 t
ORIGIN      
        1 attgggattg gtgtcaggga tatccggcac gccagtggca ttggaaaaac gcacggtcac
       61 aggggtctct gcctgctgca aatgcgcagc cttgctcagg ctggcggctg tggacgccgg
      121 cgtgaaggtg ccggtggcga cgatgccttt tgcgtggttg gcgcggaaac ccttgtgcgg
      181 cccctgcgcc agcgtggtca tgacgtcgac gatctgctcg acgaccggtt tgtcgctggc
      241 ggattccgcc gcaggcgcag gaaatgccag cagcagggag gcgcacatgg cgcctacgcg
      301 gaaaatataa tgggaagggc gcatgatgtt cctcgacagg ttgaaggaga tttcagaaag
      361 caagacggta ctggtcgcca gtttttgtcc tgggccgacg attgtcaacc atcaatatga
      421 caaatcgtcc gctagccggg acatgccgac gagcgattgg ccttattctc gcaacccacg
      481 taaaataaag gctttcgtgc tcacctaaaa tccagattcg gcagcgacgc gtgatccagc
      541 tcatcatcaa cggcaacccc cggcaattcg acacctcacg acttaatgtc gctgaactgg
      601 tcgacaagct cggtctcaca ggcaagcgcc ttgcgatcga gtgtaacggc gagatcgtac
      661 cgcgtagcca gttcgccgag tcgcccttgc atgacggcga caagctggag atcgtgggcg
      721 cagtcggagg cggttaatca atgcatacac ttactatcgc gggtaacacc tatcaatcgc
      781 gtctgctggt cggcaccggc aagtacaagg atttcgccga aacgcgcgcg gcgatcgatg
      841 ccagcggcgc tgagatcgtg acggtggcta tccggcgtac caatatcggc cagaacgctg
      901 gcgaaccttc actgctggat tacctgccgc cggacgagtt tacctacctg ccgaataccg
      961 ccggttgcta caccaccgac gatgcggtgc gtacccttag gctcgcacgc gaactgctgg
     1021 acgggcacaa gctggtgaag ctcgaggtgc tgggcgaccc caatacgctc tatcccaatg
     1081 tggtggagac cttgcgcgcc gccgagatcc tggtcaagga tgggttcgac gtcatggtct
     1141 acacatcaga cgaccccatc atcgccaagc agctggagga gatcggctgc tgtgccgtca
     1201 tgccgctggc ttcactgatc ggctccggca tgggtattct caatccttgg aatctgcaga
     1261 tcatcatcga gaacgccaag atcccggtgc tggtcgatgc cggggttggc accgcttccg
     1321 acgctgccat cgccatggag ctgggctgtg acggagtgct catgaacacc gccatcgctg
     1381 gcgcgcgtga cccggtgctc atggcaagcg ccatgcgcaa ggcgatcgag gcaggccgcg
     1441 aatccttcct cgccggccgt atggcgaaaa aactctattc cgcgacgccg agttccccga
     1501 cgacggggct catctcttga aagacgcctg atgccaacgc acaagcgcta tgaattctct
     1561 gtcgcagtgg aaactgcttt cgtgtcggac cagtccgaca tagaccagaa ccgctacgta
     1621 ttctcttaca gcatcaccat caccaatacc gggaccgttc cggcgcaact catcagtcgc
     1681 cattgggtca ttaccgatgc tgccggcacc gtacaggaag tccgcggtct cggcgtggtc
     1741 ggcgtacagc ccttgctgaa gccgggcgaa cagttcgaat acagcagcgg caccgtgatc
     1801 aacacaccgg taggcaccat gcacggcagc tatcacatga cggctgagga cggtacccag
     1861 ttcgataccg agatcgcgcc attcacgtta tccatgccgc gagtactgca ttgaacattc
     1921 ggactaccct tgtgttgcgc gcactttgcc tctccggcgc cctgctgttg tcggcctgcg
     1981 gcggagagaa ggcgatcaag ccggaagcgg cttccgaacc ggcgaagaca gtcacacgcg
     2041 aaacaccctg ccattgtcca aagccggtcg agtatccccc gatctatctg ccggacaccc
     2101 agaaaccggc catctcaggt aaaccagaag tgcccagcgg accggattac agcatgctca
     2161 agccagggcg ctgggaagac ctggaaggga taggcgagga cgacctcagc caggcgtggg
     2221 ctgcatggca acaaagctgt tcgacgctgc gtaacaagca gccatggcaa gctgcctgcg
     2281 aggccgctag cgcagtggac agtcatgacc aggccgcgat ccagcaatat ttcaggacct
     2341 atttttccgt ctatcagacc accaatgccg acggtagcga taccggcctc atcactggct
     2401 actatgaacc cttgctacat ggcagccgtg tcaggacaga gcgttatcgc tatccactgt
     2461 atggccggcc agacgatctc atcaccgtgg atctgg
//
LOCUS       DATMTW010000260         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_90615, whole genome shotgun sequence.
ACCESSION   DATMTW010000260 DATMTW010000000
VERSION     DATMTW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90615"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /gene="glnD"
                     /locus_tag="VN084_05170"
     CDS             <1..1829
                     /gene="glnD"
                     /locus_tag="VN084_05170"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWT28880.1"
                     /translation="LNYLAKRREDRLLFDFQNDLAREMGFESTKRRRASEQLMNSFYR
                     SAKFIGLMNDILLQTLREHVHPEAHGVTRLNSRFEIRNGQLDISSTSVFQRNPSAIFE
                     SFLLMQQHPELQGMSANLLRTMHRVRNLVNRDFRQDPKNKALFIKILQQPEGITHALR
                     RMNRYGILGNYIPAFGRIVGQMQHDLFHVYTVDEHILNVLRNLRRFAIARFSHEFPLC
                     SKLFADFDKPYLLYLGALFHDIAKGRGGDHSTLGTVDARRFCRMHGLPKADGELVAWL
                     VETHLVMSSTAQKSDLSDPAVIEHFARFVGDERRLVALYLLTVADIRGTSPKVWNAWK
                     AKLLENLFLAAQRLLRGSVGGADAEVLTRQRQAISTLNYYGLKSEAYDPLWEKLGRQY
                     FLRHDAQEIAWHTRLLVSHLDTPSPIVRARLSPAGDGIQTLIYTRDRDDLFARICDFF
                     DRIGYTIVEAKVHTTKHDYALDTFLVLDNSDKSVRYRDLLNYIEFELTQKLASDAPAE
                     YRTQGRISRQVKHMPITASVHIRQERESSNHTLEIIAGDRPGLLSRIAHTLLEHGVHL
                     HTAKINTLGNRAEDTFLISGKSMRQLSDDTVKHLEATLLTQF"
BASE COUNT          394 a          618 c          497 g          353 t
ORIGIN      
        1 gcctgaacta cctggcgaag cgccgtgagg atcgcctgct attcgatttc cagaacgacc
       61 tcgcgcgcga gatgggcttc gaaagcacga aacgccggcg cgccagcgag caactgatga
      121 acagcttcta tcgcagcgcc aagttcatcg gcctgatgaa cgatatcctg ctgcagacgc
      181 tacgcgagca tgtacatccg gaggcgcatg gcgtcacacg cctcaatagt cgcttcgaga
      241 tccgcaacgg ccagctcgac atcagctcga cttcggtgtt ccagcgcaat ccgtcggcaa
      301 tcttcgagag cttcctgctg atgcagcagc atcccgaatt acagggtatg agcgccaacc
      361 tgttgcgcac gatgcaccgg gtgcgcaacc tggtgaaccg cgattttcgg caggacccca
      421 agaacaaggc cctgttcatc aagatcctgc agcagcccga aggcatcacc cacgcgctgc
      481 ggcgcatgaa tcgctatggc atcctaggca attacatccc ggcgttcggc cgcatcgtcg
      541 gccagatgca acatgacctg ttccatgtct acacggtgga tgagcatatc ctcaatgtgc
      601 tgcgtaacct gcgtcgattc gccatcgccc gcttcagcca cgagtttccg ctgtgcagca
      661 agttgttcgc cgatttcgac aagccttacc tgctctacct cggcgccttg ttccacgata
      721 tcgccaaggg ccggggcggc gatcactcca ccctgggcac ggtcgacgcg cggcgattct
      781 gccgcatgca cggcttgccg aaagcagatg gcgaactggt ggcctggctg gtcgagaccc
      841 atctcgtcat gtcgagcacc gcgcagaaat ccgacctctc cgatccagcc gtcatcgaac
      901 acttcgctcg tttcgtcgga gacgagcggc gccttgtcgc gttatatctt ctgaccgttg
      961 ccgatatccg cggcaccagc cccaaggtct ggaacgcctg gaaggccaag ctgctggaga
     1021 acctgttcct ggccgcccag cgcttgttgc gtggttctgt cggcggcgcc gacgctgaag
     1081 tcctcacccg acagcgtcag gcgatctcga cgctcaatta ctatgggctt aagagcgaag
     1141 cctacgatcc gctctgggaa aagctcgggc ggcaatattt cctgcggcat gacgcccagg
     1201 agatcgcatg gcacacgcgc ctgctggtga gccatctcga tacgccctcg cccatcgtgc
     1261 gcgcacgact cagcccggcg ggtgatggca tccagaccct aatctacacg cgcgaccgcg
     1321 atgatctttt cgcacgcatc tgcgacttct tcgatcgcat cggctacacc atcgtcgaag
     1381 ccaaggtcca cacgaccaag cacgattacg ccctggacac cttcctggtg ctggacaaca
     1441 gcgacaaatc cgtgcgttat cgcgatctgc tcaattacat cgaattcgag ctgacccaga
     1501 aactggcttc cgacgcgcct gcggaatatc ggacgcaagg tcggatcagc cgccaggtca
     1561 aacacatgcc aatcaccgcc agtgtgcata tccgccagga gcgtgaatcg tccaaccaca
     1621 cgcttgagat catcgccggc gaccgccccg gcctgctctc gcgcatcgca cacaccctgc
     1681 tcgaacatgg ggtgcatctg cacaccgcca agatcaacac gctgggcaac cgtgccgagg
     1741 atacattcct catctccggc aagagcatgc gccagttgtc ggatgacacc gtcaagcatc
     1801 tggaagcgac cctgctcacc cagttctgaa ggccaccata cgtgctctgg acaaaataaa
     1861 ac
//
LOCUS       DATMTW010000261         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_224848, whole genome shotgun sequence.
ACCESSION   DATMTW010000261 DATMTW010000000
VERSION     DATMTW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224848"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..227
                     /locus_tag="VN084_05175"
     CDS             78..227
                     /locus_tag="VN084_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28881.1"
                     /translation="MQDTQHDALAEISQRLATINRNMLKVFWIVTVFALLYFGLMLYL
                     LFFRA"
     gene            241..630
                     /locus_tag="VN084_05180"
     CDS             241..630
                     /locus_tag="VN084_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HWT28882.1"
                     /translation="MLSLYLIAATVGAMLFFIGVVAPTVFGVLSAPVASTFLRRFFPR
                     YFVVLGIITFIAGLFAGDLPVRIICFVCAFLFLFSRIILTPVINQSSDIHDQRRFKLL
                     HGASVVINAAQLLMLIYVLYRYQPLQG"
     gene            complement(800..1162)
                     /locus_tag="VN084_05185"
     CDS             complement(800..1162)
                     /locus_tag="VN084_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28883.1"
                     /translation="MARIKHTVSTLVVSALALGFSLSASATPHIASVLDKPDTFLNLD
                     VNEALFTDPVATASNFIQEFAATQLSGYTPSVETFAGPYRLFSTDASTKLAETGSSGM
                     MLACLGLMLLMARKRMSL"
     gene            complement(1349..>1896)
                     /locus_tag="VN084_05190"
     CDS             complement(1349..>1896)
                     /locus_tag="VN084_05190"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWT28884.1"
                     /translation="RQLAAGYALYGTSTMLVLTTGQGVDGFTLDEATGDFMGTHPQMR
                     IPPETAEFAINMSRQRLWTAPVQRYVTECLQGAAGPRGKDFNMRWVASMVAEVHRILV
                     RGGVFMYPMDDKLRAQGGRLRLMYEANPMAMLVEQAGGLAIDGSARILDLQPTALHQR
                     VPVLLGSRYEIDYLSRLHQAR"
BASE COUNT          384 a          593 c          545 g          374 t
ORIGIN      
        1 aacttggcac gggtaaaact gacttacgct ggtagccgca cgcatcacgc tcaaccagat
       61 catcaagggg aaagaccatg caagacacgc aacacgacgc actcgccgag atcagccagc
      121 gactggcgac catcaaccgc aacatgctca aggtattctg gatcgtcacc gtatttgccc
      181 tgctctattt cgggctgatg ctctatctgc tgttcttccg cgcctgaggc cgggcccgcc
      241 atgctgagcc tttacctcat cgccgcgacc gtcggcgcca tgctgttctt catcggggtc
      301 gtcgcaccca ccgtcttcgg cgtgctgtcg gcgccagtgg ccagcacctt cctgcgccga
      361 ttcttcccgc gctatttcgt cgtgctgggc atcatcacct tcatcgccgg gctatttgcc
      421 ggggatctac cggtacggat catctgcttc gtctgcgcgt tcctctttct gttctcgcgc
      481 atcatcctca cgccagtgat caaccagtca tccgatatcc atgaccagcg acgattcaag
      541 ctgctgcatg gggccagcgt cgtcatcaac gccgcccagc tgttgatgct gatctacgtg
      601 ctctaccgct accagccttt gcaaggctga gcgctaggca cggccgatac cgcatccagg
      661 ccgatcgccg ggcacaaaaa aagcccgcgt tgcgcgggct gagtgggggg aagattaagc
      721 agggggaaat caaacggcgc gatgagtacg ccgcacgatc ccgggcgccc ggggatgagg
      781 cgccgggatc gttgcctggc tagaggctca tgcgcttgcg cgccatgagc agcatcaggc
      841 cgaggcaagc cagcatcatg ccgctactac cggtttcagc cagcttggtc gaggcatcgg
      901 tcgagaaaag ccggtatggg ccggcaaaag tctccaccga gggcgtgtaa ccgctgagtt
      961 gcgtcgcggc gaactcctgg atgaagttgg atgccgtcgc gacaggatcg gtgaacaagg
     1021 cttcgttcac gtccaggttg aggaaggtgt ccggcttgtc gagcacggag gcgatatgtg
     1081 gcgtggccga ggcggatagg ctaaagccca gcgccagagc ggataccacg agagtcgata
     1141 cagtatgttt gatgcgagcc atcttgctag tctcctttcg atttcagata agcaatcagc
     1201 ttcttatcgt cgcgagacca ttaaacccga gcggcaaaat gtgaccaata gtccatccgg
     1261 actaatgcgt gaaatgagta acggcgcggt gtttcagggt gttttagggc ggctggaaac
     1321 gtctgcaaaa cccgacgttt tcgttccctc agcgagcttg atgcaggcgc gacaggtagt
     1381 cgatctcgta ccgtgaaccg agcaggaccg gcacccgctg atgcagcgcc gtcggctgca
     1441 gatcaaggat acgcgcgctg ccgtcgatcg ccaggccacc agcctgctcg accagcatgg
     1501 ccatgggatt ggcctcgtac atcagccgca acctgccgcc ctgcgcacgc agcttgtcgt
     1561 ccatcggata catgaacacc ccaccgcgga ccaggatacg atgcacctcg gccaccatcg
     1621 acgccaccca gcgcatattg aaatccttgc cgcgcgggcc agccgcaccc tgcaggcatt
     1681 ccgtcacata tcgctggacc ggtgctgtcc acaggcgctg gcgcgacatg ttgatggcaa
     1741 attccgcagt ctccggcggg atacgcatct gtggatgggt acccatgaaa tcgccagtcg
     1801 cttcatccag cgtgaatcca tccacacctt gccccgtggt gagcaccagc atggtcgaag
     1861 taccgtatag cgcataacca gccgccagct gtcgcg
//
LOCUS       DATMTW010000262         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_661246, whole genome shotgun sequence.
ACCESSION   DATMTW010000262 DATMTW010000000
VERSION     DATMTW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661246"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..615)
                     /locus_tag="VN084_05195"
     CDS             complement(67..615)
                     /locus_tag="VN084_05195"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWT28885.1"
                     /translation="MGNRLSKIYTRTGDQGTTGLGDGSRVDKDSLRVEAMGDVDELNA
                     IIGIMLTEPILVGMRETFTAIQHDLFDLGGEICLPGHNLIKPERVTQLENALDTWNEE
                     LEPLKEFILPGGSRAAAYCHLARTVCRRAERQLVRLSREEPVTEVSLQYMNRLSDLLF
                     VACRSLNKAAGQDDVLWKNQAG"
     gene            731..>1550
                     /gene="murI"
                     /locus_tag="VN084_05200"
     CDS             731..>1550
                     /gene="murI"
                     /locus_tag="VN084_05200"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWT28886.1"
                     /translation="MNNRENNSELPVTAAHDPTQPIGIFDSGVGGISVLKHIHALLPA
                     EHLLYFADSLHAPYGNKTPEFICERAFAITDFLIAQDVKALVVACNTATAAAVDKLRA
                     HYTLPIIGMEPAVKPAAAASQNGVIGVLATVGTLKSAQFAALLEHYGQHVRVVTQACP
                     GLVECIERGELQAEATRQLVAGYVTPLLDAGVDTIVLGCTHYPFVRELIAEIAGSQVA
                     LIDTGAAVARQLMHRLEGNSLLRERSGRGVVKFWTNSPASDAEQVIVSLWGGAAE"
BASE COUNT          296 a          465 c          480 g          309 t
ORIGIN      
        1 gttaggcgtg gtgcgggcaa aagaaaaggc gccctggggc gccttgtggg tggcgtgacg
       61 ctgaggtcag ccggcctggt tcttccataa cacgtcatcc tggccggcag ccttgttcag
      121 gctgcggcag gcgacgaaca gcagatcgga cagacgattc atgtattgca acgagacctc
      181 ggtgacgggt tcctcacggc tcagcctcac cagctgccgc tctgcgcgac ggcagacggt
      241 acgcgccagg tggcagtaag cggcggcgcg gctgccgccg ggcaggatga attccttgag
      301 cggctccagt tcctcgttcc aggtatcgag cgcgttctca agttgcgtga cgcgctcggg
      361 cttgatgagg ttgtggcctg gcagacagat ctcgccgcca aggtcgaaga gatcgtgctg
      421 gatggcggtg aaagtctcgc gcatgccgac caggatcggc tcggtgagca tgatgccgat
      481 gatggcgttg agttcgtcga catcgcccat ggcttcgacg cgcagactgt ccttgtcgac
      541 gcgactaccg tcgccgagcc cggtcgtgcc ctggtcgccg gtgcgagtgt agatcttgct
      601 taaacgattt cccatgctgg tatccctgat gatgacgctg attcgatcag cacattgtag
      661 ccgatagtcc tgatcggcaa tggtagcctg tagccacttc agataaactt tgcgcacgca
      721 taagcccact atgaataacc gcgagaataa cagcgaactt cccgtgactg ccgcccacga
      781 tcccactcag cccatcggca tcttcgactc cggcgtcggc ggcatctccg tactcaagca
      841 tatccacgcg ctgctcccgg cggagcattt gctctacttc gccgattcgc tgcacgcacc
      901 ttacggcaac aaaacgccgg aattcatctg cgaacgtgcg ttcgccatca ctgacttcct
      961 catcgcgcag gacgtgaagg cgctggtggt cgcctgcaac accgcgacag cggcggcagt
     1021 ggacaagcta cgcgcgcatt acacattgcc tatcatcggc atggagcctg ccgtcaaacc
     1081 ggctgcagcc gccagccaaa acggcgtcat cggcgtcctg gctacggtcg gtacattgaa
     1141 gagcgcccag ttcgccgcat tgctggaaca ctacggccag catgtgcgtg tcgtcacgca
     1201 ggcctgcccg gggctggtgg aatgcatcga acgtggtgag ctgcaggccg aagccacaag
     1261 acaactggtc gcaggctatg tcacgccttt acttgatgct ggcgtggata ccatcgtgct
     1321 cggctgtacg cattacccct ttgtacgcga actcattgcc gagatcgccg ggtcgcaggt
     1381 cgcgctcatc gacaccggcg ccgccgtcgc gcgtcagctc atgcaccggc tggaaggcaa
     1441 cagtttgctc agggagcggt caggtcgagg tgtggtgaaa ttctggacga atagcccggc
     1501 atccgatgcg gaacaagtga tcgtcagttt gtggggtggt gcggcggaag
//
LOCUS       DATMTW010000263         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_191361, whole genome shotgun sequence.
ACCESSION   DATMTW010000263 DATMTW010000000
VERSION     DATMTW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191361"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1518)
                     /gene="pmbA"
                     /locus_tag="VN084_05205"
     CDS             complement(178..1518)
                     /gene="pmbA"
                     /locus_tag="VN084_05205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HWT28887.1"
                     /translation="MTETSFSYSRQDLQQMGEDVLRIARAAGASDAEAEISLGFGQNV
                     SVRMGETETIEYNRDKGVSVTVYFGQRRGHASTSDLSRKALEDTVAAACNIARFTALD
                     PFCGLADANLMAQQMPDLDLHHPWHITVDDALALAIECETAARAVDARITNSEGASVS
                     AYEGLFVYANTHGFNAGYPSSRHGISCSVIAEADDAMQRDYWYSTARDAADLDTAAEI
                     GREAGERTVRRLGPKRIKTAQVPVLFEAPLAAGLISQLVSAISGGNLYRKSSFLLDSL
                     GKQVASPLLNIQEDPFIRKGLASAAFDSEGVATRARTLVQDGVLQGYLLSSYSARKLG
                     MQSTGNAGGNHNLIVRSTGEDFAGLLRRMDTGLLVTELLGHGLNMVTGDYSRGAAGFW
                     VERGVIQHPVEEITIAGNLREMLNQIVAIGSDVLVQGSKQVGSILIERMTVAGD"
     gene            1573..2106
                     /gene="yjgA"
                     /locus_tag="VN084_05210"
     CDS             1573..2106
                     /gene="yjgA"
                     /locus_tag="VN084_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HWT28888.1"
                     /translation="MKPNELDDSPEAGPLSKTKRKAAMDALQELGVTLVGLPRDKLAK
                     LELPEQLLDAINEAKRITANGATRRQMQYIGKLMREVDTDPIREQLARWEGKNVAENA
                     HFHRLERWRTRLIEEEPASAEWLSQYPQTDSQQLRTLIRNARREQAANKPPKSSRELF
                     RLLREISEAEAPTDDAQ"
     gene            2125..>2510
                     /locus_tag="VN084_05215"
     CDS             2125..>2510
                     /locus_tag="VN084_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HWT28889.1"
                     /translation="MSANIINLAAAIIFALALIHTFTAKLFEALAHRHPSHAGLFHLL
                     GEVEVVFGFWSFILIIVMALLAGGTEALDYLETRHYTEPLFVFVIMVVAASNPVLLSV
                     KHAIGWIARIVPTDTTLAQTWLCLAVV"
BASE COUNT          524 a          809 c          727 g          450 t
ORIGIN      
        1 ctgcctcctt gtaatggaga tgcttgacgc ctagctctag gtaagccagg gcatcacatg
       61 ccgaaatatt agggatttat atcaggtcgt gtcttgtgca acagctacca catagcgcat
      121 ttgggtgggc agtcgatcac acccggcgtc tcgcagctgg cgctggcaaa ccgtctatca
      181 atcgccggcg accgtcatgc gctcgatgag gatggaaccg acttgcttgg agccctgcac
      241 cagcacgtcg ctgccgatcg cgacgatctg attcaacatc tcgcgcagat tgccggcgat
      301 ggtgatctct tccaccgggt gctggatcac gccgcgctcg acccagaagc cggcagctcc
      361 gcgtgaatag tcaccagtga ccatgttcag gccatgcccc agcagctcgg tcaccagcag
      421 gccggtatcc atgcgtctga gcaagcctgc aaaatcctct ccggtggagc gcacgatcaa
      481 gttatggttg ccgccggcat tacccgtgct ttgcatgccc agcttgcgcg cggagtaact
      541 cgatagcaga tagccttgca acacgccgtc ctgcaccaga gtacgggcac gggtcgccac
      601 accctcactg tcgaacgctg cgctggcgag acctttgcgg atgaatggat cctcctggat
      661 attgagcagt ggcgaagcca cctgcttgcc gaggctatca agcaggaacg acgatttgcg
      721 atagaggttg ccgccggaga tggccgacac cagttgagag atcaggccag cggccagtgg
      781 tgcctcgaac aagaccggta cctgggcggt cttgatgcgc ttgggtccca gccgccgcac
      841 cgtacgctcg cccgcctcgc gaccgatctc ggccgcagta tcgaggtcgg cagcgtcacg
      901 cgccgtgctg taccagtagt cgcgctgcat ggcgtcatcg gcttcggcga tcaccgaaca
      961 gctgatgccg tgtctcgagc tcgggtagcc ggcattgaaa ccatgggtat tggcgtaaac
     1021 gaacagcccc tcataggccg aaaccgacgc accctcggaa ttggtgatgc gggcatccac
     1081 ggcacgtgct gcagtttcgc attcgatggc gagcgccagg gcatcatcga ccgtgatatg
     1141 ccagggatga tgcaagtcga gatccggcat ttgctgcgcc atgagattgg catcggccag
     1201 accgcagaac ggatccagcg cggtgaagcg ggcgatattg cacgcggccg cgacggtgtc
     1261 ttcgagcgcc ttgcgcgaga ggtcggaggt gctggcatga ccacgccgct gaccgaaata
     1321 gaccgtgacg gagacaccct tgtcgcgatt gtattcgatg gtttcggttt cgcccatgcg
     1381 taccgagacg ttctggccga agcccaggct gatctcagcc tcggcatcgc tggcgcccgc
     1441 ggcccgggcg atacgcaaga catcctcgcc catctgctgc aggtcctgcc gggaataact
     1501 gaaactggtt tccgtcacga aaaggcgctc acaaaaaagc tgttatcata ccctgccaga
     1561 aagcacacag ccatgaaacc caacgaactc gacgattccc cggaagcggg acccctcagc
     1621 aagaccaaac gcaaagccgc gatggatgcc ctgcaggaac tgggcgtgac actggtcggc
     1681 ctgccccggg acaaactggc caagctcgaa ctcccggagc agctgcttga cgccatcaac
     1741 gaagcgaagc gcatcaccgc caacggtgcg actcgtcgcc agatgcaata tatcggcaag
     1801 ctgatgcgcg aggtcgacac tgaccctatc cgcgaacaat tggcacgctg ggaaggcaag
     1861 aacgtcgccg agaacgcgca cttccatcgc ctcgagcgct ggcgcacacg gctgatcgag
     1921 gaagagccgg cttccgcgga atggctgtcc caatacccgc aaaccgatag ccagcaactg
     1981 cgcactctga ttcgaaacgc gcggcgggag caagccgcca acaagccacc caagagcagc
     2041 cgcgaactgt tccgcctgtt acgcgagatc agcgaggcag aagccccgac cgacgacgcg
     2101 caatagcgcc acccaaggat ccgcatgtca gccaacatca tcaacctcgc tgccgccatc
     2161 atcttcgccc tagcgctgat tcacaccttc accgccaagc tgttcgaggc ccttgcccat
     2221 cgccatccca gccacgcagg tctattccac ctgctgggcg aggtcgaggt ggtgttcggc
     2281 ttctggtcgt tcatcctcat catcgtcatg gcgctgctgg caggcggcac cgaggcgctc
     2341 gactacctgg agacgcgcca ttacaccgaa ccgctgttcg tcttcgtcat catggtggtc
     2401 gctgcctcca acccggtcct gctgagcgtg aaacatgcca taggctggat cgcgcgcatc
     2461 gtgccgacgg ataccaccct ggcacagacc tggctatgcc tcgccgtcgt
//
LOCUS       DATMTW010000264         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_91222, whole genome shotgun sequence.
ACCESSION   DATMTW010000264 DATMTW010000000
VERSION     DATMTW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91222"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..662
                     /gene="rplJ"
                     /locus_tag="VN084_05220"
     CDS             138..662
                     /gene="rplJ"
                     /locus_tag="VN084_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWT28890.1"
                     /translation="MSLNLEEKKAVVAEVSAQVANAQAIILAEYRGLEVGDMTQLRSQ
                     ARKSGVYLRVLKNTVVRRAVDGTPFSGLASDMVGPLVFGISADPVAAAKVLNDFAKAN
                     DKFVIKAGAMPNQVMNASGVQALASMPSREELLAKLLGTMQAPVAKFVRTLNEVPTSF
                     VRGLAAVRDQKQAA"
     gene            707..1093
                     /gene="rplL"
                     /locus_tag="VN084_05225"
     CDS             707..1093
                     /gene="rplL"
                     /locus_tag="VN084_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWT28891.1"
                     /translation="MALSKVEILDAIASMSVLDLSELIKEMEEKFGVSAAATAVAVAA
                     APAAAGGAASAAAEQTEFNVILTSAGDNKVNVIKAVRELTSLGLKEAKDLVDGAPKAI
                     KEGVNKADADAALKKLEEAGAKAEIK"
     gene            1220..>4402
                     /gene="rpoB"
                     /locus_tag="VN084_05230"
     CDS             1220..>4402
                     /gene="rpoB"
                     /locus_tag="VN084_05230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWT28892.1"
                     /translation="MSYSFTEKKRIRKSFAKRESVQEVPYLLAMQLESYAAFLQANVA
                     PEKRLEQGLQSTFSSVFPIVSHSGNARLDYVSYNLGAEPFDVKECQQRGLTYAAPLRV
                     KVRLTIMDKEASKPTVKEVKEQEVYMGELPLMTENGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDYLYFRVDRRRKMPVTILLKALG
                     YTPEQILETFYDFDTFHIAKKGVQFELVPERLRGEVAKFDILDNDGNVIVAKDKRITV
                     KHIRDMEKAGISKITVPEEFLLGRSLAAGVVDKETGEVVANANDEITESLLDKLREAS
                     VSKITTLYANDLDHGDYISQTLRIDEIPDQYSARVAIYRMMRPGEPPTEEAVQALFDG
                     LFFNPERYDLSSVGRMKFNRRAFPEKIEERTASWLRRFYERVGPQGAEGAGTLSNDDI
                     LAVIGVLVELRNGRGEIDDIDHLGNRRIRSVGELAENQFRAGLVRVERAVKERLSQAE
                     SDNLMPHDLINAKPVSSAIREFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRE
                     RAGFEVRDVHSTHYGRVCPIETPEGPNIGLINSLALYSRTNQYGFLETPYRRVDGNKV
                     SDKIDFLSAIEESQYMIAQANSELDAKGQFKEELISARYHNEFTMAQPERVQYMDVAP
                     GQIVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRAEKAVVGTGIERTVAVDSGTV
                     VTARRGGIVDYVDSGRIVVRVHDAEAAAGEVGVDIYNLTKYTRSNQNTNINQRPLVKM
                     GDVIARGDVIADGASTDMGELALGQNMLVAFMPWNGYNFEDSILISERVVADDRYTSI
                     HIEELSVVARDTKLGAEEITRDISNLSERMLGRLDESGIIYIGAEVEAGDVLVGKVTP
                     KGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGMSGTVIDVQVFTREGIERDTRAQQ
                     IIDDQLSHYKKDLADQMRIVEDDAFGRIRRLLEGKTATGGPKKLKKGEAVTAEYLNDV
                     PRHDWFDIRLADEEAARQLEQQKDSLAQARV"
BASE COUNT          971 a         1302 c         1261 g          868 t
ORIGIN      
        1 ctctggctga tgtggattga tacctacgca gatggcgcat cccgaaacag gatcagttac
       61 tcctgatgaa aggtcgccgt gggaaggctt ttgcaggcgc aggtttgtag aagcttaatt
      121 aacggaagga gaagaccttg agtcttaatc ttgaagagaa aaaggcagtg gttgctgagg
      181 tcagcgcgca agtcgctaac gcacaggcaa tcattcttgc tgagtatcgt ggcttggaag
      241 tgggcgacat gacccagctt cgttcgcaag ccagaaaatc gggtgtctac ctgcgcgttc
      301 tgaagaacac ggtggtgcgc cgtgccgttg acggcacccc gttctctggt ctggcgagcg
      361 acatggtcgg tccgctcgtg ttcggcatct ctgccgatcc agttgccgcg gcaaaggtat
      421 tgaatgattt tgccaaggcg aacgacaagt tcgtgatcaa ggccggtgcg atgccgaatc
      481 aggtgatgaa cgcttctggt gttcaagccc tggcatccat gcctagccgt gaagagttgc
      541 tcgccaagct gctcggtacc atgcaagccc cagtggccaa gttcgttcgc acgcttaatg
      601 aagtgccgac gagctttgtc cgtggtctgg ctgcggtacg cgatcagaag caagccgctt
      661 aacgcagcaa tcgatattca acttaactat ttatcaggag tcacaaatgg ctttatctaa
      721 agtagaaatt ctggatgcga tcgcgtccat gtccgtactg gacctgtccg aactgatcaa
      781 ggaaatggaa gagaagttcg gcgtttccgc cgctgcgacc gcagttgctg ttgctgcagc
      841 accagctgct gctggtggcg ctgcttctgc agccgctgag caaaccgagt tcaacgttat
      901 cctgacctct gctggcgaca acaaggtcaa cgtgatcaag gctgttcgcg aactgacctc
      961 cctgggtctg aaggaagcca aggacctggt tgacggcgca cctaaggcga tcaaggaagg
     1021 cgttaacaag gctgatgccg acgctgccct gaagaagctg gaagaagctg gcgccaaggc
     1081 tgaaatcaag taataggcag taagtgttag acaggctggc ggaaattccg ccagccttca
     1141 ccatttttag gatggtataa gagagcgcgc tggaaacagc gctttgtctt ctgccctcac
     1201 acccccctca ggagatgcta tgagctattc ctttaccgag aaaaaacgca ttcgcaaaag
     1261 tttcgccaag cgcgagagcg tgcaggaagt cccatacctg ctcgccatgc aattggaatc
     1321 ctatgcggct ttcctgcaag ccaatgtcgc gccagaaaag cgcctcgaac aaggtctgca
     1381 gtcgacgttc agctcggtgt tcccgatcgt cagtcattcc ggcaatgcac gcctggacta
     1441 tgtcagctac aaccttggcg ccgaaccgtt cgacgtcaag gaatgccaac agcgtggcct
     1501 gacgtatgcc gcaccgctgc gtgtgaaggt gcgcctgacc atcatggaca aggaagcttc
     1561 caagcccacc gtcaaggaag tgaaagaaca agaagtctac atgggtgaat tgccgctcat
     1621 gaccgagaat ggctccttcg tcatcaacgg taccgagcgc gtcatcgtgt cccagctgca
     1681 ccgcagcccc ggcgtgttct tcgagcacga tcgcggcaag acccatagct cgggcaagct
     1741 gctgttctcc gcacgcatca tcccttaccg tggttcctgg ctcgacttcg agttcgatcc
     1801 caaggactac ctgtatttcc gcgtcgatcg ccgtcgcaag atgccggtca ccatcctcct
     1861 gaaggcgctg ggttatacgc cagaacagat cctcgaaacg ttctacgatt tcgatacctt
     1921 ccatatcgcc aagaagggcg tgcagttcga gctggtgcct gaacgtctgc gcggtgaagt
     1981 cgccaagttc gatatcctcg acaacgacgg caatgtcatc gtcgccaagg acaagcgcat
     2041 caccgtcaag cacatccgcg acatggaaaa ggccggtatc agcaagatca ccgtacctga
     2101 agaattcctg ctgggccgct cgctggcagc tggtgtggtc gataaagaga ctggtgaagt
     2161 cgtggccaac gccaacgacg agatcaccga aagcctgctc gacaagctgc gcgaagccag
     2221 cgtctccaag atcaccacgc tgtacgccaa cgatctggat catggggatt acatctccca
     2281 gaccctgcgc atcgacgaga tcccggacca atacagcgcg cgcgttgcca tctatcgcat
     2341 gatgcgccct ggcgagccgc ccaccgaaga agctgtgcaa gcactgttcg acggcctgtt
     2401 cttcaatccg gaacgttacg acctgtcgtc cgtcggccgc atgaaattca accgtcgcgc
     2461 cttcccggaa aagattgaag agcgcactgc ctcctggctc cgccgcttct atgagcgcgt
     2521 tggtccgcaa ggcgccgaag gcgccggtac gctctccaat gacgacatcc tggccgtgat
     2581 cggcgtgctg gtcgagctgc gcaacggtcg tggcgagatc gacgatatcg accacctggg
     2641 taatcgccgt atccgttccg tcggtgaatt ggctgagaac cagttccgtg ccggtctcgt
     2701 ccgtgtcgaa cgcgccgtca aggagcgtct gagccaggcc gaatccgaca acctgatgcc
     2761 gcacgacctg atcaacgcca agccggtatc gagtgcgatc cgcgagttct tcggttcttc
     2821 gcaactgtct cagttcatgg accagaccaa tccgctgtcc gagatcaccc acaagcgtcg
     2881 tgtctcagca ctgggccctg gtggcctgac ccgtgagcgc gccggtttcg aggtgcgtga
     2941 cgtgcattcg acgcactacg gtcgtgtgtg tccgatcgag acgcctgaag gtccgaacat
     3001 cggtctgatc aactcgctgg cactgtattc ccgtaccaat cagtatggct tcctggaaac
     3061 gccttaccgt cgtgtcgacg gcaacaaggt gtcggacaag atcgacttcc tgtccgcgat
     3121 cgaggaaagt cagtacatga tcgcgcaggc gaactccgag ctggacgcga agggtcagtt
     3181 caaggaagaa ctgatctccg cgcgttacca caacgaattc accatggcgc agccggaacg
     3241 cgtgcaatac atggacgtgg cccctggcca gatcgtctcc gttgccgcgt cgctgatccc
     3301 gttccttgaa cacgatgacg cgaaccgtgc actgatgggc gccaacatgc aacgccaggc
     3361 tgttccttgt ctgcgcgccg agaaggctgt cgtcggtacc ggtatcgagc gtactgtcgc
     3421 agtcgactcc ggcaccgtgg tcaccgcacg ccgtggcggt atcgtcgatt acgtcgattc
     3481 cggccgtatc gtggtgcgtg tgcacgatgc agaggccgct gccggcgaag tcggcgtgga
     3541 catctacaac ctgaccaaat acacccgttc caaccagaac accaacatca accagcgtcc
     3601 gctggtcaag atgggtgacg tcatcgcccg tggcgatgtc atcgccgacg gcgcatcgac
     3661 cgacatgggc gaactggcac tcggccagaa catgctcgtg gccttcatgc catggaacgg
     3721 ctacaacttc gaagactcga tcctgatctc cgagcgcgtc gtcgcagacg accgttacac
     3781 ctcgatccac atcgaggagc tgtcggtcgt ggcgcgtgac accaagcttg gtgccgaaga
     3841 gatcacccgt gacatctcca acctgtccga gcgcatgctc ggtcgcctgg acgaatccgg
     3901 catcatctac atcggtgctg aagtggaagc cggcgacgta ctggtcggta aggtcacgcc
     3961 caagggcgag acccagctca cgccggaaga gaaattgctg cgcgcgatct tcggcgaaaa
     4021 ggcctccgac gtgaaggaca cctcgctgcg cgtgccgtcc ggcatgtccg gcacggtcat
     4081 tgacgtgcag gtcttcacgc gtgaaggtat cgaacgcgat acgcgcgctc agcagatcat
     4141 cgacgaccag ctgtcccact acaagaagga cctggccgac cagatgcgta tcgtcgaaga
     4201 cgacgcgttc ggccgtatcc gccgcctgct ggaaggcaag accgccaccg gcggcccgaa
     4261 gaagctgaag aagggcgagg ctgtcactgc ggaatacctg aatgacgttc cgcgccacga
     4321 ctggttcgac atccgtctgg ccgacgagga agccgcgcgt cagctggaac aacagaagga
     4381 cagcctggca caggcgcgtg tc
//
LOCUS       DATMTW010000265         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_225321, whole genome shotgun sequence.
ACCESSION   DATMTW010000265 DATMTW010000000
VERSION     DATMTW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225321"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VN084_05235"
     CDS             <1..445
                     /locus_tag="VN084_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HWT28893.1"
                     /translation="QPERDTVFADFYARFKDYPLVVDKWFSLQATANRDAIFEDFAAL
                     RRHAEFNIKNPNRVRALYTAFAINNPVRFHDPSGQGYALLRDVIIELNALNPQMASRL
                     VMPFREWKRYTPSLQALMRDALQKIMATPNLSNDVFEVVSKSLEA"
     gene            542..1540
                     /gene="gap"
                     /locus_tag="VN084_05240"
     CDS             542..1540
                     /gene="gap"
                     /locus_tag="VN084_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWT28894.1"
                     /translation="MTTRVAINGFGRIGRMVFRAAIKDFTDIEVVAINDLLEPDYLAY
                     MLKHDSVHGKFQGEVKVDGNHLVVNGKKIRLTAEKDPANLKWNEVGADIVIECTGFFL
                     TQETCQKHIDAGAKKVVQSAPSKDDTPMFVYGVNHDAYKGEAIVSAASCTTNGLAPVA
                     KVLHDNFGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPALNKKLTGMAFRIPTSDVSVVDLTVELEKETTYDAICAVMKQASESGPLKGVLGY
                     TNEKVVSTDFRGETCPSVFDADAGIQLDPTFVKVVAWYDNEYGYTCNLMRLVRHIA"
     gene            1720..>2738
                     /locus_tag="VN084_05245"
     CDS             1720..>2738
                     /locus_tag="VN084_05245"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWT28895.1"
                     /translation="MSVIKMTDLDLKGKRVFIRADLNVPVKDGKVTSDARITASMPTI
                     EHALKAGAKVMVTSHLGRPEEGVYSEENSLQPVADVIAAKLGKPVRLIKDWVDGGFEV
                     AEGELVLLENCRFNKGEKKSLDETSQKYARLCDIFVMDAFGTAHRAEASTHGIAKFAP
                     VAAAGILLVGELEALTKALLNPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGI
                     ANTFLKAAGHEVGKSLCEDDLVPTAKMLMDKMSARGAAVPIAVDVVVGKQFDANEPAV
                     LKDADSVAADDMIFDIGPKSAKELADIIMSAATVVWNGPVGVFEFDQFADGTKTIAEA
                     IAKTKA"
BASE COUNT          580 a          799 c          799 g          560 t
ORIGIN      
        1 ccaaccggaa cgggatactg tatttgccga tttttacgcc cgcttcaagg attacccgct
       61 ggtggtcgac aagtggttct cgctacaggc aacggccaac cgtgacgcca tctttgagga
      121 tttcgccgcg ctgcgccggc atgccgagtt caacatcaag aaccccaatc gcgtgcgcgc
      181 gctgtatacc gcgttcgcca tcaacaaccc ggtgcgattc cacgatccca gcggccaggg
      241 ttacgccctg ctgcgcgacg tcatcatcga gctcaacgcc ctcaatccgc agatggcatc
      301 gcgtctggtg atgcccttcc gcgaatggaa acgctatacc ccgagcctgc aggcactcat
      361 gcgcgatgcc ctgcagaaga tcatggcgac gccgaatctg tcgaacgatg tgttcgaggt
      421 ggtgagcaag agcctcgaag cctagtccgg cggccctctg cgggcttgca tgtggtagga
      481 taacggtctt ggccaaagtt attttctata ttcaaatcaa cgtgtcacag gaaaaagcgt
      541 tatgactacc cgtgttgcta ttaatggttt cggccgcatc ggtcgtatgg tattccgtgc
      601 agcgatcaag gatttcacgg atatcgaagt ggtcgcgatc aatgacctgc tggagccgga
      661 ttatctggcg tatatgctta aacatgactc cgtccacggc aagttccagg gcgaagtgaa
      721 ggtcgatggc aatcacctgg tggtcaacgg caagaagatc cgcctgactg cggagaagga
      781 tcccgccaac ctcaagtgga acgaggtcgg cgctgacatc gtcatcgaat gtacgggctt
      841 tttcctgacc caggagacct gccagaagca tatcgacgct ggcgccaaga aggtcgtgca
      901 atccgcgcct tccaaggatg acacaccgat gttcgtctat ggcgtcaatc acgatgccta
      961 caaaggcgaa gccatcgtct ccgccgcgtc ctgtaccacc aacggcctgg ccccggtcgc
     1021 caaggtcctg cacgacaact tcggcatcaa gcgtggcctg atgaccaccg tacatgccgc
     1081 cactgccacg cagaagaccg tcgatggacc atccaacaag gactggcgcg gcggtcgggg
     1141 catcctcgag aacatcatcc cgtcttccac cggcgccgcc aaggccgtgg gcaaggtgat
     1201 ccccgcgttg aacaagaaac tgaccggcat ggcgttccgt atccccactt ccgatgtctc
     1261 cgtggtcgat ctcacggtag agcttgagaa ggagactacc tacgacgcga tctgtgccgt
     1321 catgaagcag gcctccgaaa gcggcccgct gaagggtgtg ctcggctaca ccaacgagaa
     1381 ggtggtctcc accgactttc gtggcgaaac ctgcccttcg gtgtttgacg ctgatgccgg
     1441 tatccagctc gacccgacct tcgtcaaggt tgtcgcctgg tatgacaacg agtacgggta
     1501 tacctgtaac ttgatgcgcc tcgttcgcca catcgcttga tgcaacggct gcacgggcga
     1561 atcgctttgt tactcggatg ctgcaaaaac tcgccgtact aactgtactg tcttcgtttt
     1621 tgtgcgctcc gagcgcctag cgtttcatcc cgttcgctcg ccgcctcgtt ccgatccgtt
     1681 catcggagtt gagctacaac tagtttttgg gagtagaaca tgtcagtaat caaaatgacc
     1741 gacctggatc tgaagggcaa gcgcgtcttc atccgcgccg acctcaatgt gccggtcaag
     1801 gacggcaagg tcacctccga tgcccgcatc accgcgtcca tgccgacgat cgaacatgcg
     1861 ctcaaggccg gggcaaaggt catggtgact tcccatctgg gccggccgga agagggcgtc
     1921 tactccgaag agaattcgct gcagccggtg gccgacgtca ttgctgccaa actcggcaag
     1981 cctgtgcgcc tgatcaagga ctgggtcgat ggcggtttcg aggtcgctga aggtgaactc
     2041 gtgctgctgg agaactgtcg tttcaataag ggcgagaaga agagcctcga tgaaacgtca
     2101 caaaagtacg ccaggctgtg cgatatcttc gtcatggacg ctttcggcac tgcgcaccgt
     2161 gctgaagctt cgacccacgg catcgccaag ttcgcgccgg tcgcggcggc cggcatcctg
     2221 ctggtgggcg agctcgaggc cttgaccaag gccttgttga acccggcgcg cccgatggtg
     2281 gcgatcgtcg gcggctccaa ggtcagtacc aagctcaccg tgctggagag cctgtcggag
     2341 aaggtcgatc aattggtggt cggcggcggt atcgccaaca ccttcctgaa ggcagcgggt
     2401 cacgaagtcg gcaagtcgct gtgcgaggac gatctggtgc caacggcaaa gatgctgatg
     2461 gacaagatgt ccgcgcgtgg tgcagcggtg ccgatcgctg tggatgtcgt ggtcggcaaa
     2521 cagttcgatg ccaacgagcc tgcggtattg aaagacgcgg atagcgtggc cgctgacgac
     2581 atgattttcg atattggccc gaaatctgct aaagaattgg cagacattat catgagcgca
     2641 gcgacggtcg tctggaatgg tcctgtcggc gtgttcgagt tcgaccagtt tgccgatggc
     2701 accaagacca tcgccgaagc catcgccaag accaaggc
//
LOCUS       DATMTW010000266         5790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_459989, whole genome shotgun sequence.
ACCESSION   DATMTW010000266 DATMTW010000000
VERSION     DATMTW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459989"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..937)
                     /locus_tag="VN084_05250"
     CDS             complement(23..937)
                     /locus_tag="VN084_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HWT28896.1"
                     /translation="MNFLALLGALALSHYRPHSRLDWLQQIFSPYARVLERNFNGGKN
                     RHGVLAWLLAVFLPVLLLGVGYWVVHEISPSLGILCGIVMLYATLRFSQFGKRAEQIV
                     TALRNDDLAQARQLVSSWEGNDASQYSAAELAKVSIEIMLRRAHQGLFAPIFWFVILG
                     PAGALLYRLSHLTTLAWQGSEDTLFNHFARRAFEYLDWLPARITAGSFAVVGDFEDAV
                     YCLRTQAESWPDKILGIVLASGAGALGVKIGEPIPHNGVIEFRPEIGMGDEADADYLQ
                     SSIGLVWRVLVLMVGLLLLLTFAHWLGN"
     gene            complement(934..1470)
                     /locus_tag="VN084_05255"
     CDS             complement(934..1470)
                     /locus_tag="VN084_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HWT28897.1"
                     /translation="MKKQLPYFLIVFLVPVIAVLWWWGLFSGAKVEIAERGGYRYAYL
                     DAQGVYSKLGKKRNEVLFTLDQQHIKAGAEITLMLSDPRTTPHEELKAQTGFIIDADA
                     TPQAPLKVATIPPRQVAVAEIRAHPLFAYGKAYSALLEYAKTHDTTLHLPTLEIFHDS
                     KLTVEMPLQAETAPGVMP"
     gene            1637..2491
                     /gene="purU"
                     /locus_tag="VN084_05260"
     CDS             1637..2491
                     /gene="purU"
                     /locus_tag="VN084_05260"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWT28898.1"
                     /translation="MKNTATLLITCPDQKGIVAAIADFLYRHNANILHSDQHQDAESN
                     LFLMRVEWDLSGAAIQADDFSQHFGPIAERFQMQWQLKLSQKRTRVAIMVSQYDHCLA
                     DLLHRHQGGELACEIPLIISNHRDTERLAGFYGIPFHHIEVTKDTKAEAEARQFALFD
                     QYQIDLIVLARYMQILSPDFVQRYPRRIINIHHSFLPAFIGARPYHRAYERGVKLIGA
                     TSHYVTEVLDEGPIIEQDIARISHRDQVEDLIQKGRDLERIVLSRAVRWHIENRILPY
                     ANKTVIFD"
     gene            complement(2541..3785)
                     /gene="lysA"
                     /locus_tag="VN084_05265"
     CDS             complement(2541..3785)
                     /gene="lysA"
                     /locus_tag="VN084_05265"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWT28899.1"
                     /translation="MNPFHLKNGVLHAETAALPDIAARYETPTYVYSRKALEASFTAF
                     QSGFVGSDHLICFAVKSNPSLAILNLFARMGAGFDIVSGGELARVLAAGGDPAKVVFS
                     GVGKTATEMRAALTAGIFCFNVESAAELDRLNAVAGELGKTAPVSLRINPNVDAKTHP
                     YISTGLKNNKFGVAYEDALMLYQRAASMPNIAIHGVDCHIGSQITELSPFLDAFDRVL
                     VLVDQLQAVGIDIHHIDAGGGIGICYSDETPPDFTAYAAAMRAKLGDRNLKLVFEPGR
                     ALVGNAGLLLTKVEYLKHTEAKNFAIVDAAMNDLMRPALYDAYHNIQAVTPHTGTPEV
                     YEIVGPVCESGDFLGHDRKLALREGDLLAILSAGAYGMSMSSNYNTRPRAAEVMVDGD
                     SCHLIRQRETVDQLFALEKTLA"
     gene            4128..4556
                     /locus_tag="VN084_05270"
     CDS             4128..4556
                     /locus_tag="VN084_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HWT28900.1"
                     /translation="MRARLAQHSSGFTLIELMVVVAIVGVLASIAMPAYSRYVQRSKA
                     AEATSALSDWRNRMERFFQDNRTYTGACAAATPTGIKYFTMSCVETAQTYTLTATGKA
                     DQGMSGFTFTLDQNNSKQTTAYPGSTVPANCWLTKQGGSC"
     gene            4571..5083
                     /locus_tag="VN084_05275"
     CDS             4571..5083
                     /locus_tag="VN084_05275"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HWT28901.1"
                     /translation="MGFTIIELMIGIAIIAILLAIALPSFQSMILGIQVKSAAEALNN
                     GLQLAKAEAVRRNNNVTFALGTGTAWTVGCTIPIADSNADGVDECPAVIQSRPNEIGS
                     GNVTLAVSPAGATTVTFNGLGRTVAANPIARMDVAAVGTNKILRVQISGGSIRMCDPT
                     IVSTANARKC"
     gene            5086..5538
                     /locus_tag="VN084_05280"
     CDS             5086..5538
                     /locus_tag="VN084_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilV"
                     /protein_id="HWT28902.1"
                     /translation="MSAISNNELSSKSDKQSGSVMIEALVAILIFSMGILAIVGLQGV
                     AIKTVADSQYRLEASFFANRVVSDIWTNVANINDYVLPGGGATGLDTWLAEVQSRLPG
                     ATGANAPTITIAANPSGGFTATIAIMWQAPGESAPHNFTTVAYINPNP"
     gene            5544..>5790
                     /locus_tag="VN084_05285"
     CDS             5544..>5790
                     /locus_tag="VN084_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWT28903.1"
                     /translation="MAMKQSQTGFSLIEIMVGMTISLIGMVVIMQVFTVAEGNRATTT
                     TGGDAQQSGALALQSVERDVRMAGYGINIPGFLGCNVV"
BASE COUNT         1247 a         1635 c         1690 g         1218 t
ORIGIN      
        1 agaaggattt ccataccggg actcaattac ctaaccagtg ggcgaaggtc agcaacagca
       61 acagaccgac catcaatact agcacacgcc agaccaggcc gatcgaactc tgcagatagt
      121 cggcatccgc ctcgtcgccc ataccgattt ccggccggaa ctcgatcacc ccgttatgcg
      181 gaatcggttc gcctatcttg acgccaagcg caccggcgcc gctcgccagc acgatgccga
      241 ggatcttgtc cggccagctt tcggcctggg tacgcaagca atagacggca tcctcgaagt
      301 cgccgaccac tgcgaagctg cctgcagtga tgcgcgcggg cagccagtcg aggtactcga
      361 aagcgcgtcg cgcgaagtgg ttgaacaggg tgtcttcgct gccttgccat gccagtgtcg
      421 tcagatgcga gaggcggtag agcagtgcgc ccgcagggcc caggatgacg aaccagaaga
      481 tgggggcgaa cagaccctga tgcgcgcgcc gcaacatgat ctcgatgctg accttggcca
      541 gctcggccgc ggaatattgg ctggcgtcgt taccttccca gctcgacacc agttgccggg
      601 cctgggcaag gtcatcgttg cgcagggccg tgacgatctg ctcggcgcgt ttgccgaact
      661 ggctgaagcg cagggtggcg tagagcatga cgatgccgca gaggatgccg agtgatgggc
      721 tgatctcatg tactacccag taaccgacgc cgagtaacag caccggcagg aagaccgcca
      781 gcagccatgc gagcacgcca tggcggttct tgccgccgtt gaagttgcgt tccagtacgc
      841 gcgcgtaggg cgagaatatc tgctgcagcc agtccagccg actgtgcggg cgataatggc
      901 tcagcgccag cgcaccgagc agggcgagga agttcatggc atcactccgg gagcagtttc
      961 ggcttgcagg ggcatctcga cggtgagctt gctgtcatgg aagatctcca gcgtcggtag
     1021 atgcagcgtc gtatcgtggg tcttggcgta ctcgagcagc gccgagtagg ctttgccgta
     1081 tgcgaacagc ggatgggcgc gaatctcggc gaccgcgacc tggcgtgggg gtatggtggc
     1141 gactttcagt ggcgcctggg gtgtggcgtc ggcatcgatg ataaagccag tctgcgcttt
     1201 cagttcttcg tgcggcgtgg tcctggggtc gctgagcatg agcgtgatct ctgcaccggc
     1261 cttgatatgc tgctgatcga gcgtgaacag cacttcgttg cgcttcttgc caagtttgga
     1321 atagacgccc tgggcgtcga ggtaggcgta gcgatagcca ccgcgttcgg cgatctcgac
     1381 cttggcgccg gagaacaggc cccaccacca gagcacggcg atcaccggca caagaaaaac
     1441 gatcaggaaa tagggtagtt gctttttcat ggatggagtt cgatagactt aaaacctgcg
     1501 cgcacggcaa aaaaccgatg ccggttgcaa gataacatat agggcgatgg cttgcacttg
     1561 ttcggggctc gcggacttac ttgatataat ggcgtttttc ccatcattgc cagcttcgta
     1621 gcggattgca aacaccatga aaaacacggc cactctgctg atcacctgcc cggaccagaa
     1681 aggcatcgtc gcagccatcg cggatttcct ctaccggcac aatgccaaca tcctgcattc
     1741 ggaccagcat caggatgccg agagcaatct cttcctcatg cgcgtcgagt gggacttgag
     1801 tggcgccgcc atccaggccg acgatttcag ccagcatttc ggcccgatcg ccgaacgttt
     1861 ccagatgcag tggcagctca agctgtcgca gaaacgtacg cgtgtggcga tcatggtctc
     1921 tcaatacgat cattgcctgg ccgatctgtt gcaccgtcat cagggtggcg agctggcctg
     1981 tgagatcccg ctcatcatca gcaatcatcg tgacaccgaa cgactggcgg gtttctatgg
     2041 catcccgttc catcatatcg aggtcaccaa ggacaccaag gccgaagccg aagccaggca
     2101 attcgccctg ttcgatcaat accagatcga tcttatcgtg ctggcgcgtt acatgcagat
     2161 cctgtcgccg gatttcgtac agcgctatcc gcggcgcatc atcaacatcc atcactcgtt
     2221 cctgccggcg ttcatcggcg cacgacccta tcaccgtgcc tacgaacgcg gcgtcaaact
     2281 gatcggtgcc accagccact atgtcacgga ggtgctggac gaaggcccga tcatcgagca
     2341 ggacatcgca cgtatctcgc atcgcgacca ggtggaagac ctgatccaga aggggcgtga
     2401 cctcgaacgt atcgtgctat cgcgtgcggt acgctggcac atcgagaacc gcatcctgcc
     2461 ttacgccaac aagaccgtca tcttcgattg atcgtcacgg gctgatagcc tctggcgggt
     2521 cgcgccgtgt gcgcgatcgt tcaggcgagt gtcttttcca gcgcgaacag ctgatccacg
     2581 gtctcgcgtt gccgaatcaa gtggcagctg tcgccatcga ccatcacctc ggccgcacgc
     2641 ggacgcgtgt tgtagttgga gctcatgctc atgccgtagg ctccggctga caggatggcg
     2701 agcaggtcgc cttctcgtag cgcaagcttg cggtcgtgcc cgaggaaatc cccgctctcg
     2761 cagactggcc cgacgatctc gtaaacctcc ggcgtgcccg tgtgcggcgt caccgcctgg
     2821 atgttgtggt atgcgtcata cagtgcgggg cgcatcaggt cgttcatcgc cgcatcgaca
     2881 atggcgaaat tcttggcctc ggtatgcttg aggtattcca ccttggtcag cagcaggccg
     2941 gcattgccga cgagcgcgcg ccccggttcg aacaccagtt taaggttgcg gtcgcccagc
     3001 tttgcacgca tggccgcggc gtaagcagta aagtcaggtg gcgtctcgtc gctgtagcag
     3061 atgccgatcc caccgccggc atcgatatga tggatgtcga tgccaaccgc ttgtagctga
     3121 tcgaccaata ccagcacgcg atcgaaggca tcgaggaagg gcgataactc ggtgatctgt
     3181 gagccgatat gacaatcgac gccatggatc gcgatattgg gcatgctggc cgcgcgctga
     3241 tagagcatga gcgcatcttc ataagccacg ccgaacttgt tgttcttgag cccggtggag
     3301 atgtagggat gcgtcttggc gtccacattc gggttgatac ggagggatac cggcgctgtc
     3361 ttgccgagct cgccggcgac tgcattcaac cggtccagct cggccgcgga ctcgacgttg
     3421 aagcaaaaga tgccggccgt cagggccgca cgcatctccg tcgctgtctt gccgacaccg
     3481 gagaacacca ccttggcagg atcgccgccg gcggccagta ctcgcgccaa ttcgcccccg
     3541 gacacgatat cgaatcccgc gcccattctc gcgaagaggt tgaggatcgc gaggctgggg
     3601 ttggacttga cggcaaaaca gatcagatgg tcactgccga cgaagccgga ctggaaagcc
     3661 gtgaaactcg cttcaagcgc cttccgggaa tacacgtagg tcggcgtctc atagcgcgcc
     3721 gcgatgtcgg gcaaggcggc cgtttcggca tgcagcacac cgttcttaag atggaatggg
     3781 ttcacggatg atgatttcgg aatggtggtt tattcattgg tatcgtgttt gggatcgctt
     3841 ttgggagcgt cctgccgatc ctgcgggtac tgtttctctg gcaagtacag cggccccttg
     3901 gtgccacagg cagtcaagat caaacatagt atgatgctgg tcaggatagc gcgcaccatg
     3961 ggcctcacga ttcgttcaga ttgcgatgcc ggattttaac ataagggttg ccgcaaccga
     4021 ttttgtcgag cgatgccgtc cataacctga atctcaccga ttatcggcaa gatgtggatg
     4081 ttggcgtcaa acttgttcaa tattcataga ccttaaaggg agtcatcatg cgtgcacggt
     4141 tagctcaaca ttccagcgga ttcacactaa tcgagttgat ggtcgtcgtt gcgattgtcg
     4201 gcgtcctcgc ttcgatcgca atgccggctt actcaagata tgtccagaga agcaaggccg
     4261 cagaagcgac cagcgcactt tctgattggc gcaatcggat ggagcgcttc tttcaggata
     4321 atcgaaccta tacgggcgcc tgtgccgcag cgaccccgac cgggatcaaa tatttcacca
     4381 tgagctgtgt cgaaaccgcc cagacttata cgctcaccgc aaccggcaaa gctgatcaag
     4441 gcatgagcgg cttcacgttt accctcgatc agaataacag caagcaaacc acggcttacc
     4501 ctggttccac ggtgcctgct aactgctggc tgaccaagca aggtggtagt tgctgatgcg
     4561 atccaagtcc atgggattta ccatcatcga actgatgatc ggtattgcaa tcatcgctat
     4621 attattggcg atcgcattac ctagttttca atcgatgatc ctgggtatcc aggtcaagtc
     4681 cgctgctgaa gcactcaata acggtttgca gctcgctaaa gccgaggcag tcaggagaaa
     4741 taacaacgtc actttcgccc ttggtacggg tacggcttgg accgtgggct gtacgatacc
     4801 cattgcagat agtaatgcag atggtgtcga cgaatgcccg gccgtgattc aaagccgccc
     4861 taatgagatt ggttccggca acgtaacatt ggcggtcagt cctgcagggg cgacgacggt
     4921 gactttcaat ggtcttggcc ggacagtagc agctaacccg attgcccgca tggacgtcgc
     4981 tgctgtcggg actaacaaga ttttgcgcgt ccaaatcagt ggtgggagta tccgcatgtg
     5041 tgatccaact atcgtcagca ctgcgaatgc gaggaaatgc tgaaaatgtc tgcgatatcc
     5101 aacaacgaac tctcatcgaa aagcgacaag caaagcggtt ctgtaatgat cgaagcgctg
     5161 gtcgcgatcc tgatcttctc gatgggcata ctggcgattg tcggcttgca gggtgtggcc
     5221 atcaaaacag tggcggactc ccagtatcga ctcgaagcga gtttttttgc gaacagggta
     5281 gtttcggaca tctggaccaa cgtcgccaac atcaatgact atgtcctgcc tggtggcggc
     5341 gcaacaggac tagacacctg gctggcagaa gtccaatcca gattacctgg cgccaccggc
     5401 gccaatgcgc caacgatcac cattgccgcc aacccatctg gcggcttcac ggcaacgatt
     5461 gcgatcatgt ggcaagcgcc gggcgaatcc gcaccgcata atttcaccac agtcgcttac
     5521 atcaacccca acccttgatc gaaatggcga tgaaacaatc acagacaggc tttagcctca
     5581 tcgaaatcat ggtcggcatg accatcagcc tcattggcat ggttgttatc atgcaggttt
     5641 ttactgtggc cgagggaaat cgagctacga ctaccactgg gggagatgcg cagcaaagcg
     5701 gtgcattggc tttgcaaagt gtggaacggg acgtacgtat ggcgggctat ggtatcaaca
     5761 ttccgggttt tcttggttgt aatgtggtcg
//
LOCUS       DATMTW010000267         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_325185, whole genome shotgun sequence.
ACCESSION   DATMTW010000267 DATMTW010000000
VERSION     DATMTW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325185"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="der"
                     /locus_tag="VN084_05290"
     CDS             <1..1104
                     /gene="der"
                     /locus_tag="VN084_05290"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWT28904.1"
                     /translation="QRPVLLAVNKTEGIQRAIASAEFHELGLGEPLSISSAHGEGVRD
                     LVELALENVPAEIETEASEENTKHPKIAIVGRPNVGKSTLVNAMLGEERVIAFDQPGT
                     TRDSIHIELERGGKHYTIIDTAGVRKRGKVIEAIEKFSVIKTIQAIEEANVAVLVVDA
                     QEGITEQDAHVSAYILESGRALVVAINKWDGLEDDRRDWIKREIDRKLQFLDFAQFHY
                     ISALKKKGLPELFKSIDIAYKAAMAKLSTPQLTRVLEDATTLHQPPISKNIRPKLRYA
                     HQGGSNPPIIVIHGNHVDGIRDNYIKFLEKTFRKAFQLTGTPLRIQFNQGENPFAQRE
                     NKPKRGEGIVSMRRRKTAQRAELKARKQDKDKD"
     gene            complement(1202..2005)
                     /locus_tag="VN084_05295"
     CDS             complement(1202..2005)
                     /locus_tag="VN084_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWT28905.1"
                     /translation="MKHSLALIAVLWLTGCAIFGAPTEIDETKGWPVQRIFSEAEQKM
                     RDKDYEKAIKYYQTLESRYPHGRYATQAQLEIAYAYYKKNDPASCIAAADRFIKLHPN
                     HPNIDYAYYIKGLATFTERGLVEKLTKQQISDRDPKSMRESFSALKELVTRYPNSRYV
                     KDATLRMTYLVNALAEHEMHVARYYMKRKAYVAAINRAKYVVEVYPDSTSVEEALVIL
                     ISAYDLLGMDDLRDDTHRVLQTNYPNSRMLGKGVPSDQQVWWKFWESLF"
     gene            2043..>2629
                     /locus_tag="VN084_05300"
     CDS             2043..>2629
                     /locus_tag="VN084_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWT28906.1"
                     /translation="MTQPTPITLTIPAELGGQRLDQVLQQLLPEYSRSRLQAWIKEGQ
                     VKLGEQAVTPKLKVWGGEQVVVMPQTVLSDQPFQPQDIPLNIVHEDADILVINKPAGL
                     VVHPAAGNWEGTLLNGLLHHLPALERVPRAGIVHRLDKDTSGLLVVAKHLEAQTHLVR
                     QLQARTVKREYRAIVWGQIWRNGTVDQPIGRHPHA"
BASE COUNT          595 a          732 c          776 g          526 t
ORIGIN      
        1 cagcgcccgg tattgctggc ggtgaacaaa accgaaggta tccagcgggc gatcgcaagt
       61 gccgagttcc atgaacttgg tctaggcgag ccgctatcca tctcctcagc acatggcgaa
      121 ggtgtgcgcg atctggtcga gctggctctc gaaaacgtgc cggcagagat cgagacggaa
      181 gcgagtgagg aaaacaccaa gcaccccaag atcgcgatcg tcgggcggcc gaatgtcgga
      241 aagtcgacgt tggtgaatgc catgctcggc gaagagcgcg tgatcgcttt cgaccagcca
      301 ggcaccacgc gcgattcgat ccatatcgag ctggaacggg gcggcaaaca ttacacgatc
      361 atcgataccg ctggtgtacg caagcgcggc aaggtgatcg aagcgatcga gaagttctcg
      421 gtgatcaaga ccatccaggc gatcgaggag gcgaatgtgg ccgtgttggt ggtcgatgcc
      481 caggaaggca tcaccgagca ggatgcgcat gtttccgctt atatcctgga gagtggccgt
      541 gccttggtcg tcgccatcaa taaatgggat ggtctggaag acgaccggcg tgactggatc
      601 aagcgcgaga tcgatcgcaa gctgcaattc ctggattttg cccagttcca ttacatctcg
      661 gcgctgaaga aaaagggttt gcctgagttg ttcaagtcca tcgacatcgc ctacaaggct
      721 gccatggcca agttatcgac gccgcagctg acccgtgtac tggaagacgc cacgaccttg
      781 catcaacccc cgatcagcaa gaacatccgc cctaagctgc gatacgcgca ccaggggggt
      841 agcaacccac cgatcatcgt gattcacggc aaccatgtgg atggtattcg cgataattac
      901 atcaagttcc tggaaaagac tttccgcaag gccttccagt tgactggtac gccgctccgc
      961 atccagttca atcaaggcga gaacccgttc gcgcagcgcg agaacaagcc gaaacggggc
     1021 gagggtatcg tcagcatgcg tcgccgcaag acagcgcagc gagccgaatt gaaggcccgc
     1081 aagcaagaca aggacaagga ttaacctaga acggcgcgcg tcgaatggct gctgtgtagc
     1141 agccattcat caagcgacgg tatgacaggg caggggcgag gaagaactgt cccgtgctat
     1201 ttcagaacag actttcccag aatttccacc agacttgctg atcgctgggc acacctttgc
     1261 caagcatcct gctgttcgga tagttggttt gcagcacgcg gtgcgtatca tccctgaggt
     1321 catccatgcc gagcagatcg taggcactga tgaggatcac cagcgcttcc tcgacactgg
     1381 tcgaatccgg atagacttcc accacatatt tggcgcggtt gatcgcagcg acataggcct
     1441 tgcgcttcat gtagtagcgc gccacatgca tctcatgttc ggccagcgca ttgaccagat
     1501 aggtcattcg caaggtagcg tccttgacgt aacgactgtt cgggtaacgc gtgaccagtt
     1561 ccttcagggc agaaaaagac tcgcgcatgg atttcggatc ccggtcactg atctgctgct
     1621 tggtcagctt ttcgacgagg ccacgttccg tgaatgtggc caggcccttg atgtaatagg
     1681 cgtaatcgat attcggatgg ttggggtgca gcttgatgaa gcggtcggcc gccgcgatac
     1741 aggacgccgg atcgttcttc ttgtaatacg cataggcgat ctcgagctgt gcctgggtgg
     1801 catatcggcc atggggatag cgggactcga gcgtctggta gtacttgatg gctttttcgt
     1861 agtccttgtc gcgcatcttt tgctcggctt cggaaaagat acgctgaacc ggccaaccct
     1921 tggtctcgtc gatttcagtc ggcgcgccga agatggcgca gcccgtgagc cagagcaccg
     1981 caattaacgc taaactatgc ttcattgaat acgccccaaa aaaattcaga agattataac
     2041 cgatgaccca gcccactcct atcactctta ccattcccgc cgagctcggt ggacagcggc
     2101 tggatcaggt cttgcagcag ttattgccgg agtattcaag gtcgcgcctg caagcctgga
     2161 tcaaggaggg gcaggtcaag ctgggcgaac aggctgtgac gccgaaactc aaggtttggg
     2221 gtggggagca ggtcgtcgtc atgccgcaga cggtgctatc ggaccagccg ttccagccgc
     2281 aggacatccc tctcaacatc gtgcatgagg acgcggacat cctcgtcatc aacaagcctg
     2341 cagggcttgt ggtccatcca gcggcaggga attgggaagg caccttgctc aacggcttgt
     2401 tgcaccatct gcctgcattg gagcgtgtgc cgcgcgcggg catcgtccat cggctggaca
     2461 aggacaccag cggcctgctg gtcgtcgcca agcatcttga agcccagacc caccttgtgc
     2521 ggcaattgca ggcgagaacg gtcaaacgcg aatatcgcgc catcgtctgg ggccagatct
     2581 ggcgtaacgg cacggtcgac cagccgatcg gacgccaccc gcatgccag
//
LOCUS       DATMTW010000268         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_58152, whole genome shotgun sequence.
ACCESSION   DATMTW010000268 DATMTW010000000
VERSION     DATMTW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58152"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..>2046
                     /locus_tag="VN084_05305"
     CDS             211..>2046
                     /locus_tag="VN084_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HWT28907.1"
                     /translation="MAKTVALLLGVHAHQPVGNFESVLDDAHQRCYGPFLRVLHQYPE
                     FRFAIHISGWLLEYMLKHFPQDMALLKEMVEREQAELFGAGYTEPVLASIPSADRVGQ
                     ITQLSAYLKQKLGEAPSGAWLTERVWESSVVPSLADSGIRYVTVDDYHFMCAGKGDAQ
                     LNGYYSTEEDGRKIDLFPISEALRYRLPFSPANEVVGYLESLADDSKQSAAIYFDDIE
                     KFGIWPETYEWVYERGWLKDFIEGVLNSPVIKPMRYSDYHAMAKTRGVVYLPTTSYIE
                     MNEWTLPVPAAHHYADLVAQEKYAGRYELTKPYVRGGIWRNFFMRYPESNWMHKRMLA
                     LSRRYHGLPANKQTPEMLACLYEAQANDAYWHGLFGGLYLPHLRRAVYRAIIRLEALL
                     DKVAERPQKVTGDLDLDGHDEVFLQNGDIQAILRLDGTASICEFDSYRLSHNFGDTLT
                     RQAEHYHRKVHAQPEHGHDGEGIANPHERVSFKHEITVADLATDTYRKSLFCDSWLMP
                     EGEAIRPNYRKPVIGKAVDFKAALRGGEIHKKIAIGDAALVVTYGFTRPPAGSFRVEI
                     NLAMPSCDGPAGRFRIGDNIPGGFGQTFKFGGLKNILLEDEVLGGA"
BASE COUNT          415 a          624 c          596 g          411 t
ORIGIN      
        1 gtcgacagct tcgaccgtct ctaccggcgc aacctttcca atctctatcg cctgctcaag
       61 ctgaccatcc cggaggtcct caatcgaccg atcagtgtcg gtgccggtga cccggcggcg
      121 gccggcacca tgcgtcgcgg ccaggaaacc tgagcgaggt cgtcatgcgt acaacatcat
      181 caaacagcat caacttgtgg agagccgatc ttggctaaga ccgtcgcatt gttactgggc
      241 gtgcatgccc accagcctgt gggcaatttt gaaagcgtcc tcgatgacgc gcaccagcgc
      301 tgttacggcc cgttcctgcg ggtcctgcac caatacccgg aattccgttt cgccatccat
      361 atcagcggat ggctgctcga atacatgctc aagcattttc cccaggatat ggcgctgttg
      421 aaggagatgg tcgagcgcga gcaggccgag ctgttcggcg ccggctatac ggagccggtg
      481 ctggcttcga tcccttcagc cgatcgggtc gggcagatca cgcagttatc cgcctacctc
      541 aaacaaaagc tgggcgaggc gccttccggc gcctggttga ccgagcgtgt atgggaatcc
      601 tcggtcgtgc catcgcttgc ggattccggc atccgctatg tcacggtcga cgattaccat
      661 ttcatgtgcg caggcaaggg cgatgcgcag ctcaatggct actactcgac agaagaggat
      721 gggcgcaaga tcgacctgtt cccgatctct gaagccttgc gttaccggtt gccgttctct
      781 ccggcgaacg aggtcgtcgg ttacctggag agcctggccg acgacagcaa gcaatccgcg
      841 gccatctatt tcgacgatat cgagaaattc ggcatctggc ccgaaaccta cgagtgggtc
      901 tacgaacgcg gctggctcaa ggatttcatc gaaggcgtac tgaattctcc ggtcatcaag
      961 ccgatgcgct acagcgacta ccatgccatg gccaagaccc gcggcgtggt ctacctgccg
     1021 accacttctt atatcgagat gaatgaatgg accttgccgg tgccggcagc ccatcactat
     1081 gccgatctgg tcgcacagga aaaatacgcc ggccgttacg aactgaccaa accctatgtc
     1141 cgtggcggta tctggcgcaa tttcttcatg cggtatccgg agtcgaactg gatgcacaag
     1201 cgcatgctgg ccctgtcgcg tcgttatcat ggcctgccag ccaacaaaca gacgccggag
     1261 atgctggcct gcctgtatga ggcgcaggcc aacgacgcct actggcacgg tctcttcggt
     1321 ggcctttacc tgccgcatct gcggcgtgcc gtctatcgcg ccatcatcag gttggaagca
     1381 ttgctggaca aggtcgccga acggccgcaa aaggtcacgg gcgatctcga tctcgatggc
     1441 catgacgagg tgttcctgca gaatggcgat atccaggcca tcctgcggct ggatggcacg
     1501 gcctcgatct gcgagttcga cagttaccgc ctgtcgcaca actttggcga cacactgacg
     1561 cgccaggccg aacactacca tcgcaaggtc cacgcacagc cggaacacgg ccacgacggg
     1621 gaaggcatcg ccaatcctca tgagcgggtc agtttcaagc atgagatcac agtcgccgat
     1681 ctcgcaacgg atacttatcg caagagcctt ttctgcgaca gttggctcat gccggaaggc
     1741 gaggcgatcc gccccaacta tcgcaagccg gtcatcggca aggctgtcga tttcaaggcg
     1801 gcgctgcgtg gcggtgagat ccacaagaag atcgccatcg gtgatgcggc gctggtcgtc
     1861 acctacggct tcactcgccc gcctgccggc agtttccgag tcgagatcaa tctcgccatg
     1921 cccagttgcg atggaccggc agggcgcttc cgcatcggtg acaacatccc tggcggtttt
     1981 ggccagacct tcaagttcgg cgggctgaaa aacatcttgc tggaagacga ggtgctgggt
     2041 ggggcg
//
LOCUS       DATMTW010000269         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_493734, whole genome shotgun sequence.
ACCESSION   DATMTW010000269 DATMTW010000000
VERSION     DATMTW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493734"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VN084_05310"
     CDS             <1..1382
                     /locus_tag="VN084_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HWT28908.1"
                     /translation="ETDSKSDTQLGNADVLPAGASVVQIVRREKVPRERQVVRLPAYE
                     EVVRDPVLYAHASRVLHLESNPGNARALVQRHADREVWLNPPPIPLTTKEMDYVYDLP
                     YARKPHDVYEGARIPAWEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSEESILHE
                     IEEIRDKVDGFTGVISDLGGPTANMYRMACKSEAIEKACRKLSCVYPGICENLNTDHS
                     DLIQLYRKARAIKGVKKILVGSGLRYDLAVTSPEYVKELVQHHVGGYLKIAPEHSEDN
                     VLSKMMKPGMGAYDAFKAMFEKFSKEAGKEQYLIPYFIAAHPGTTDEDMLNLALWLKK
                     YDFKLDQVQTFTPTPMAMATAMYHSGKNPLRKVTADSDDVPIPKAGGVRRLHKAFIRY
                     HDPNNWPMLREALKRMGRADLIGNGKKHLVPAWQPVVPVRSGPASRTRKSEPLAKKSS
                     RGTASRGSR"
     gene            1696..2424
                     /locus_tag="VN084_05315"
     CDS             1696..2424
                     /locus_tag="VN084_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWT28909.1"
                     /translation="MTLTPAVNLLSVVVPVRNEEGNIEPLVAEIAAALSGVAEHEIIY
                     VDDGSTDSTAQRLQDLKSAYPQLRVLHHAQSCGQSTAVRSGVKAARGDWIATLDGDGQ
                     NDPADIPKLIAALEGAVVLVGGNRRHARRDTWIKRVSSVIANGVRSRMLNDSTPDTGC
                     GLKLIRRDVFLELPYFDHMHRFLPALVLRSGGQIRSIPVNHRPRERGTSNYGTIDRLL
                     VGIVDLFGVAWLLRRAKQPQVRED"
     gene            2425..>2768
                     /locus_tag="VN084_05320"
     CDS             2425..>2768
                     /locus_tag="VN084_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HWT28910.1"
                     /translation="MHHGIGFYLNHLAHRWQDYLAGLGSVDIVWLAIGLLGQTMFMMR
                     FIVQWIHSERHQKSVIPVSFWYLSLIGGLTVLAYGIRNAEPVIILGQLPGTIVYTRNL
                     MLIRREHARQAA"
BASE COUNT          552 a          876 c          793 g          547 t
ORIGIN      
        1 gtgagaccga tagcaaatcc gatacccagc tgggcaatgc cgatgtgttg cctgctggcg
       61 ccagcgtggt ccagatcgtg cgtcgcgaga aggtgccgcg tgagcgccag gtggtacgcc
      121 tgccggcgta tgaagaagtc gtgcgcgatc ccgtgctgta tgcccatgcc tcgcgtgtgc
      181 tgcatctcga gtccaatccg ggcaacgcgc gcgcgctggt ccagcgccac gccgatcgcg
      241 aggtctggct gaatccgccg ccgatcccgc tcaccaccaa ggagatggat tacgtctacg
      301 acctgccgta tgcgcgtaaa ccgcacgatg tctatgaggg cgcgcgcatc ccggcctggg
      361 agatgatccg tttctcggtc aacatcatgc gcggctgttt cggcggctgc accttctgct
      421 ctatcacaga gcacgaaggt cgcatcatcc agagccggtc cgaggaatcc atcctgcacg
      481 agatcgagga gatccgtgac aaggtcgacg gtttcaccgg cgtcatctcc gaccttggcg
      541 gccccacagc gaacatgtac cgcatggcgt gcaaatcaga agccatcgaa aaggcctgcc
      601 gcaagctgtc ttgcgtctat cccggcatct gcgagaacct caataccgat cattccgatc
      661 tgatccagct ctaccgcaaa gcccgcgcca tcaagggcgt gaagaagatc ctcgtcggct
      721 ccgggctgcg ctacgatctc gcggtgactt cgccggaata cgtgaaggaa ctggtgcagc
      781 atcatgtcgg cggttatctc aagatcgccc ctgaacattc tgaagacaat gtgctgtcga
      841 agatgatgaa gcctggcatg ggcgcttacg acgcgttcaa ggccatgttc gagaaattct
      901 ccaaggaagc gggcaaggaa caatacctga tcccgtattt cattgccgcg cacccgggta
      961 cgaccgatga ggacatgctg aacctcgcgc tatggctgaa gaaatacgac ttcaagctcg
     1021 atcaggtgca gaccttcacg ccgacgccga tggccatggc gacggccatg taccattccg
     1081 gcaagaaccc gttgcgcaag gtcacggccg acagcgatga cgtgcctatt cccaaggctg
     1141 gcggcgtgcg ccgcctgcac aaggcgttca tccgctacca tgatccgaat aactggccca
     1201 tgctgcgtga ggcattgaaa cgcatgggcc gtgccgacct catcggcaac ggcaagaaac
     1261 acctggtgcc ggcctggcag ccagtcgtgc cggtcaggtc cggccctgct tcccgcacca
     1321 gaaaatccga accgctcgcc aaaaaatctt cccgtgggac cgcatctcgc ggttcgcgct
     1381 aagcctcctt aacaccccgt tcccgcggat cccctgatcc ggcctgcaat ttttgcaggc
     1441 ctcgccgggc tatgttacgc aaactttgca tgacccagcc cggttgtaac aggattgtca
     1501 tcttttccgg ctacactcgc gcgcttcgcc ttcattgcaa cacattgata tacatcaatt
     1561 aatcaagctt catgttccgg cacactgtga gatttggtgt gtggcatgtt ccgtgctgat
     1621 tcgatgggga tgtcgtaatc gccacaacaa ccggcgtgtg cgggatgcgc acgcgaaaac
     1681 aaaggctaac ccctgatgac gctcaccccc gctgtcaacc tgctttccgt tgtcgttccg
     1741 gtgaggaacg aggagggcaa tatcgaaccc ctggtcgccg agatcgccgc tgcgctgtca
     1801 ggcgtggctg agcacgagat catctatgtg gatgacggca gcaccgatag cactgcccag
     1861 aggctgcaag acttgaagtc ggcatatccg cagttgcgcg tgttgcatca tgcccaaagc
     1921 tgcggtcaga gcaccgcggt ccgcagcggc gtgaaggcgg cccgcggcga ttggatcgcc
     1981 acgctggatg gcgatggcca gaatgacccg gccgatatcc ccaaactgat cgcggcgctc
     2041 gaaggcgccg tggtcctggt cggcggcaat cgtcggcatg cgcggcgcga cacctggatc
     2101 aagcgggtct cctcggtgat cgccaatggc gtgcgttcac gtatgctgaa cgacagcacg
     2161 cccgataccg gttgcggcct gaagctcatc cgccgcgacg tcttcctcga actaccgtat
     2221 ttcgatcaca tgcaccgctt cctgccggcg ctggtgttac gcagcggcgg ccagatccgc
     2281 tcgatcccgg tcaaccatcg tccacgtgaa cgcggcacct ccaactacgg cactatcgac
     2341 cgcctgctgg tcggcatcgt cgatcttttc ggcgttgcct ggctgctgcg ccgcgccaaa
     2401 caaccacaag tcagggaaga ctaaatgcat cacggtatcg gcttctacct caaccatctg
     2461 gcgcaccgct ggcaggatta cctcgccggt ctgggctcgg tggatatcgt ctggctggcg
     2521 atcggcctgc tcggccagac catgttcatg atgcgtttca tcgtgcagtg gatccatagc
     2581 gaacgccacc agaaaagtgt catcccggtg agtttctggt acctgtccct gatcggcggg
     2641 cttaccgtgc tcgcctacgg tattcgcaac gccgagccgg tcatcatcct gggccagttg
     2701 ccgggcacca tcgtctatac ccgtaacctg atgttgatcc ggcgcgaaca tgcgcgccag
     2761 gccgcaga
//
LOCUS       DATMTW010000270         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_325291, whole genome shotgun sequence.
ACCESSION   DATMTW010000270 DATMTW010000000
VERSION     DATMTW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325291"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..>2081)
                     /gene="uvrB"
                     /locus_tag="VN084_05325"
     CDS             complement(82..>2081)
                     /gene="uvrB"
                     /locus_tag="VN084_05325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWT28911.1"
                     /translation="VTRFEPAGDQPEAIRLMVEGIEAGLAHQTLLGVTGSGKTFSIAN
                     VIAQVQRPTLVLAPNKTLAAQLYGEFKAFFPNNAVEYFVSYYDYYQPEAYVPSSDTFI
                     EKDASINDHIEQMRLSATKALLERKDAIIVTTVSCIYGLGSPETYLKMVLHVDRGDKL
                     DQRELLRRLADLQYTRNDMEFARATFRVRGDVIDIHPAESDFEAIRIELFDDEVESLS
                     AFDPLTGEVIRKLPRFTFYPKSHYVTPRETLLDAVEGIKVELQERLDYLRSNNKLVEA
                     QRLEQRTRFDLEMILELGYCNGIENYSRYLSGREAGAPPPTLYDYLPADALLVIDESH
                     VSVPQVGAMYKGDRSRKETLVEYGFRLPSALDNRPMRFDEWESISPQTIFVSATPGNY
                     EAEHAGRVIDMVVRPTGLVDPQIEIRPALTQVDDLLSEITKRVALEERVLVTTLTKRM
                     AEDLTDYLADHGVRVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDMPEVS
                     LVAILDADKEGFLRSERSLIQTIGRAARNLNGRAILYADRITGSMERAIGETERRRDK
                     QIAFNLANGITPKGVFKDVADIMEGATVPGSRSKKRKGMAKAAEENARYEAELRSPGE
                     IAKRIKALEEKMYQLARDLEFEAAAQMRDEIAKLRERLITV"
BASE COUNT          392 a          656 c          615 g          418 t
ORIGIN      
        1 aggctcgctc ccactggaat tacgctaaac cttgtgggag cgagcctgct cgcgaagagg
       61 cccgcgcacg caacgcaaat ctcagactgt aatcaagcgc tcgcgcaact tggcaatctc
      121 gtcacgcatc tgcgccgccg cttcgaattc caggtcacgc gccaactggt acatcttctc
      181 ctccaacgcc ttgatacgct tggcgatctc acccggcgag cgcaactcgg cttcgtaccg
      241 ggcgttttcc tcggcggcct tggccatgcc cttgcgcttc ttgctgcgcg aacccggcac
      301 ggtggcgcct tccatgatat cggcaacgtc cttgaacacg cccttgggcg tgatgccgtt
      361 ggccaggttg aaggcgatct gcttgtcgcg gcgacgctcg gtttcgccga tcgcccgctc
      421 catggagcca gtgatccggt cggcatacag aatcgcccgg ccattgaggt tacgcgccgc
      481 ccgaccgatg gtctggatca gcgaacgctc ggaacgcagg aagccttcct tgtcggcatc
      541 gaggatcgcc accagcgaca cttccggcat gtccaggccc tcgcgcagca ggttgatccc
      601 caccagcaca tcgaaggtgc caaggcgcaa gtcgcggatg atctccaccc gctccacggt
      661 gtcgatgtcc gagtgcagat agcgtacgcg cacgccgtga tcggccaggt agtcagtcaa
      721 gtcctcggcc atgcgcttgg tcagcgtcgt gaccagcact cgctcttcca gggcgacgcg
      781 cttggtgatt tccgacagca aatcgtcgac ctgggtcagt gccgggcgga tctcgatctg
      841 cgggtccacc agaccggtcg gacgcaccac catgtcaatg acgcgcccag cgtgttcggc
      901 ctcataattg cctggcgtcg ccgagacaaa aatcgtctgc gggctgatgc tttcccactc
      961 atcgaaacgc atcggccgat tgtccagcgc cgatggcagg cggaagccat attccaccag
     1021 cgtctcttta cgcgagcggt cgcctttgta catggcgcct acttgcggca cgctgacgtg
     1081 ggattcgtcg atcaccagca aagcgtcggc cggcaggtaa tcgtacaggg tcggcggcgg
     1141 cgccccggct tcgcgaccgg acaggtagcg cgagtagttc tcgatgccat tgcaataacc
     1201 cagctcaaga atcatctcca ggtcaaaccg ggtgcgctgc tccaggcgct gcgcctccac
     1261 cagcttgttg ttggaacgca ggtagtccag gcgctcctgc aactcgacct tgatcccttc
     1321 gacggcatcc agcaaggttt cccgaggtgt cacgtagtgg cttttcgggt agaaggtgaa
     1381 gcgcggcagc ttgcgaatga cttcaccggt caacggatcg aaggccgaca ggctctccac
     1441 ttcgtcatcg aagagctcga tgcggatcgc ttcgaaatct gattccgccg ggtggatatc
     1501 gatcacatcg ccgcgcaccc ggaacgtcgc acgggcaaat tccatgtcgt tgcgggtgta
     1561 ttgcaggtca gccaggcggc gcagcaattc acgctggtca agcttgtcgc cacgatccac
     1621 gtgcaaaacc atcttcagat aggtttccgg gctgcccaga ccgtagatgc acgacaccgt
     1681 ggtgacgatg atcgcgtcct tgcgctccag caaagccttg gtcgcggaca gccgcatctg
     1741 ctcgatatgg tcgttgatcg aagcgtcttt ctcgatgaag gtgtccgaag acggcacgta
     1801 cgcttcaggc tggtagtagt cgtagtagga aacgaagtat tcgacggcat tgttcgggaa
     1861 gaacgccttg aattcaccgt acaactgcgc cgccagggtc ttgttcggcg ccagtaccag
     1921 ggtcgggcgc tgtacctggg cgatcacgtt ggcgatgctg aaggtcttgc cggaacctgt
     1981 cacaccgagc aaagtctggt gcgccagccc ggcctcaatg ccctcgacca tcaggcggat
     2041 ggcctctggt tgatcgccgg caggctcgaa gcgggtgact a
//
LOCUS       DATMTW010000271         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_258339, whole genome shotgun sequence.
ACCESSION   DATMTW010000271 DATMTW010000000
VERSION     DATMTW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258339"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="ftsY"
                     /locus_tag="VN084_05330"
     CDS             complement(<1..875)
                     /gene="ftsY"
                     /locus_tag="VN084_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWT28912.1"
                     /translation="MFDFLKKDKPQTAVPAIPAAESPPSWTERLKQGLAKTRSQLGNQ
                     LAGLFGGGKIDESVYEELETILLTSDIGVNTTQALLENIRGRVKRQNLSDTTQLRLAL
                     KEALLELLAPLEQPLDTATAKPFVIMLAGVNGAGKTTTIGKLAHLFQAQGKSVLIAAG
                     DTFRAAAREQLQAWGERNQVHVVAQDSGDPAAVIFDAVNSARAKGIDIVLADTAGRLP
                     TQLNLMEEIRKVQRVIDKALPGAPHEVLLVLDANTGQNAVMQVKAFDDALKVSGLVLS
                     KLDGTAKGGVIAAIA"
     gene            1040..2404
                     /locus_tag="VN084_05335"
     CDS             1040..2404
                     /locus_tag="VN084_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWT28913.1"
                     /translation="MRVIFLILALSAPLVSSLAQAADIEEFKLKNGLRLIVQEDHRSP
                     VVVQQVWYRAGAIDEVNGKSGVAHVLEHMMFKGTRKIKPGQFSRIIAAAGGRENAFTG
                     QDYTAYYQQLEKSQLPLAMKLEADRMANLQLTDAEFSKEIKVVMEERRWRTEDKPAGM
                     LNEAFNAAAYVAHPYGRPIVGWMNDLENMTAADAREWYHTWYTPNNAVLVIAGDVNAR
                     DVYKLAQKYYGPIKAHPLPTRKPQREPEQKGERRVVVKAPAKLPSLQMGFHVPALRDP
                     DHDWEPYALEILAGVLDGNSSARLNQNLVREQRIAVDVGAGYDLIQRSPDSMFELEGT
                     PSEGKTVDELEAALMQEVERIKESGVTDEELQRVKAQVIAADVYERDSTFYQSMQIGR
                     LEVSGFSWRILKDYPAKLSAVTAQQVQEVARKYLNRDNLTVATLDPQPIDPNAKPKGT
                     PHVH"
     gene            2394..>3040
                     /locus_tag="VN084_05340"
     CDS             2394..>3040
                     /locus_tag="VN084_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWT28914.1"
                     /translation="MCINRWTMWLAMLLTFAVPAHAAVKIQQWQTTSGTAVYFVENHD
                     LPILDLSVEFAAGSARDDKASSGLAGLTRHLMALGAGGLTDEAISKRMADTGAIMSGQ
                     LDADRAGFKLRTLSSEKEREAALDVFIKVLQQPDFPAPVLEREKARIIAGLKEAATQP
                     ESISDDAFMQALYGDHPYALDQDGDLETIPSLKREDLQRFYTTYYGARNAVIAMIG"
BASE COUNT          631 a          921 c          929 g          559 t
ORIGIN      
        1 tgcgcgatag cagcgatcac gccgcccttg gccgtgccgt cgagcttgct caggaccagg
       61 ccgctgacct tgagggcgtc atcgaaggcc ttcacctgca tcaccgcatt ctgccccgtg
      121 ttggcatcga gcaccagcag cacttcatgc ggcgcgccgg gtaaggcctt gtcgatgacg
      181 cgctggacct tgcggatctc ctccatgaga ttgagctgcg tgggcagacg gcctgccgtg
      241 tcggcgagaa cgatatcgat acccttggcc cgcgcggaat tgaccgcatc gaagatcacg
      301 gctgccgggt cgccactgtc ctgggccacg acatggacct ggttgcgctc cccccaggct
      361 tgcaactgtt cacgagccgc ggcgcgaaag gtgtcgccag cagcgatcag cacggacttg
      421 ccctgggcct ggaacaagtg ggcgagcttg ccgatggtgg tagtcttgcc ggcgccgttg
      481 acgccagcca gcatgatgac gaaaggcttg gcagtggcgg tgtccagcgg ctgctccagc
      541 ggcgccagca gttcgagcag agcctccttg agggcaaggc gcagctgggt ggtgtcactc
      601 aggttctgac gcttgacgcg cccgcggata ttctccagca gcgcctgggt cgtgttgacg
      661 ccgatatccg aagtcaacag gatggtttcc agttcttcgt agaccgattc gtcgatcttg
      721 ccgccgccga acagtcctgc gagttgattg ccgagctggc tacgggtctt ggccagcccc
      781 tgtttcagcc gttccgtcca ggacggcggc gattctgctg cggggatggc gggtactgcg
      841 gtttgcggct tgtctttttt gaggaaatcg aacatggtgg tcagtgtcat cgatgaacct
      901 tattttagac gcaatccccg atccatgcca cgaactaacg gcagcgctgg catgtcacag
      961 caatgcattt cgatcgtatc caccgacagg atcgaggccg cgcgcatgcg gcagtccaaa
     1021 ggagaataac aacgtgttga ttcgagtcat ctttctgata ctggcgttgt ctgcgccgct
     1081 ggtgtcatcg ttggcgcagg cggccgacat cgaggaattc aagctcaaga atgggctgcg
     1141 cctgatcgtg caggaggacc accgttcacc ggtcgtcgtc cagcaggtat ggtaccgggc
     1201 gggcgctatc gatgaagtga acggtaaatc cggtgtggcc catgtgctgg aacacatgat
     1261 gttcaagggc acacgcaaga tcaagccagg ccagttctcg cgcatcatcg ccgccgctgg
     1321 cggacgcgag aatgcgttca ctggccagga ctacaccgcc tactaccagc aattggaaaa
     1381 gtcacagttg ccgctggcga tgaaacttga ggctgaccgg atggccaacc tgcaactgac
     1441 ggacgccgag ttctccaagg agatcaaggt cgtcatggaa gagcggcgct ggcgcactga
     1501 agacaagcct gccggcatgc tcaacgaagc cttcaatgcc gcagcctatg tcgcacatcc
     1561 gtatggtcgt ccaatcgttg gctggatgaa tgaccttgag aacatgacgg ccgctgacgc
     1621 ccgtgagtgg taccacacct ggtacacgcc caacaacgcg gtgctggtga tcgccggcga
     1681 tgtgaacgcg cgcgatgtct acaagctggc gcaaaaatat tacgggccga tcaaggcgca
     1741 tccgctgcct acgcgcaagc cacagcgcga accggagcaa aagggcgagc gccgggtcgt
     1801 ggtcaaggca ccggccaagc tgccatcgct gcagatgggt ttccatgtgc ccgccctgcg
     1861 tgatcccgac catgattggg agccctacgc gcttgagatc ctggccggcg tactcgacgg
     1921 caatagttcc gcccgtctca accagaacct ggtgcgtgaa cagcgcatcg ccgtcgatgt
     1981 cggtgccggc tatgacctga tccagcgcag ccccgacagc atgttcgagc tggaaggcac
     2041 gcccagcgag ggcaagacgg tagatgagct cgaagccgca ctgatgcagg aggtggaacg
     2101 catcaaggag agcggcgtga ccgacgagga gctacagcgc gtgaaagcgc aggtcatcgc
     2161 cgccgatgtc tacgaacgtg attccacgtt ctaccagagc atgcagatcg gccgcctgga
     2221 agtcagcggg ttctcctggc gcatcctcaa ggactacccg gccaaactca gcgccgtgac
     2281 ggcacagcaa gtccaggagg tcgcaaggaa atacctcaac cgcgataacc tgacagtcgc
     2341 cacgctcgat ccgcagccga tcgaccctaa cgcaaaacca aaaggaacac cccatgtgca
     2401 ttaatcgatg gaccatgtgg ctggcgatgc tgctgacatt cgcggtgcct gcccatgccg
     2461 cagtcaagat ccagcaatgg cagaccacaa gcggcactgc tgtgtatttc gtcgagaacc
     2521 atgatctgcc gatcctggat ctcagtgtcg agttcgcggc cggtagtgcg cgcgatgaca
     2581 aggcctcctc ggggctggca ggactcaccc gtcacctgat ggcgctcggc gcaggcggtc
     2641 tcaccgatga agcgatctcg aaacgtatgg cggatacggg tgccatcatg tccggccagc
     2701 tcgatgccga tcgtgcaggc ttcaagctgc gcaccctgag cagcgaaaag gaacgcgaag
     2761 ccgcgctcga tgtgttcatc aaggtgctgc agcagccaga tttccctgcg cccgtacttg
     2821 aacgcgaaaa ggcccgcatc atcgctggcc tcaaggaagc cgcaacccag cctgagagca
     2881 tctccgacga cgcgttcatg caggcgctgt atggcgacca cccgtatgcg ctggatcagg
     2941 acggtgacct tgagaccatc ccatcgctca aacgcgagga cctgcagcgc ttctatacga
     3001 cttactatgg cgcacgcaac gcagtgatcg ccatgatcgg
//
LOCUS       DATMTW010000272         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_192140, whole genome shotgun sequence.
ACCESSION   DATMTW010000272 DATMTW010000000
VERSION     DATMTW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192140"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VN084_05345"
     CDS             <1..477
                     /locus_tag="VN084_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HWT28915.1"
                     /translation="LAAESKYDHPFQWGSKRTGPDLARVGGKYSNEWHTQHLTAPRSL
                     VPQSVMPNYPWLSATPLDYSDIEKRMLALRTTGVPYSKTNAEYERNVKEFGEVVAKSL
                     HIPDAEKNLIAQAQLGNYDTNPENITEMDALVAYLQMLGTLVDFKKYDDDYFVKFR"
     gene            514..681
                     /locus_tag="VN084_05350"
     CDS             514..681
                     /locus_tag="VN084_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HWT28916.1"
                     /translation="MEWLLWFTKFENTKPVSLVIFFVVFCGVLVYLYGNKKRGKQLED
                     YKNIPLRDDEK"
     gene            699..1637
                     /locus_tag="VN084_05355"
     CDS             699..1637
                     /locus_tag="VN084_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWT28917.1"
                     /translation="MAQQKAKGPTTTGHVWDGDLQELTNPLPRWWIWGFYLTFIFTIT
                     YWLLYPAWPIGGNYTKGVPGLNSITYTATTVDGKEVQKTTHWNMRSKFMVEMNELHAA
                     QKQWFDKVAATPYADVAQDADLMQFVNSAGKTLFSDNCAPCHQAGGQGKIGFSPNLTD
                     DHWQYGGTYEEINQTIVGGRHGYMPPFKEVLDDAQITQLANYVLSLSGEPNDSAAASA
                     GEALFHSETAACYYCHGADAKGRTVLGSANLTDKIWLWADIPSEKTAQAKVDAVKKVI
                     TGGLAKGVMPNWNERLKPEQIKLLTVYVHDGLGGGK"
     gene            1749..3185
                     /gene="ccoG"
                     /locus_tag="VN084_05360"
     CDS             1749..3185
                     /gene="ccoG"
                     /locus_tag="VN084_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HWT28918.1"
                     /translation="MEASSAKQSPLYQKHIPIFTRSVKGRFRNFKTAVMVLAYTIYFL
                     LPWMPWQRVSAADQAVLFDLGSRRFFIFDLIVYPQDIFWLAMLLFIAAALLFFATGLI
                     GRAWCGYFCFQTLWSDLFILIEKTIQGERPARMRLYNQPWNMEKILKVGGSHALMILV
                     SFWTAVTFAAYFAYAPDFVRDLFTGHAADAGYITVIALTLTTYFAAGIIREHICTLVC
                     PYARFQSVMYEADTLAVSYDARRGEGTAGRALPNAGMKKREDRQAKGHGDCIDCGYCV
                     QVCPTGIDIRNGLQYQCISCGLCIDACNNIMDSVGYPRGLIRYDSENNLHSEQPHAPR
                     LEWKRLKVIGYAAALIIATGVLFYNIGTRAATEANVQQIRQPLFIMLSDGSIRNRYQI
                     HIVNKSEQDEVYTLTVRGIPQDALDIGSIPEIKVRAGKNLNLNVKVNLSSELAKTTNR
                     FQFVVTPSGADRQPIVLDTNFYNKHQNP"
     gene            3182..3970
                     /locus_tag="VN084_05365"
     CDS             3182..3970
                     /locus_tag="VN084_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HWT28919.1"
                     /translation="MSLPILPMTETLFGGILLAAVFFFITRRAGLSNFWAAVLSGVLP
                     FLAYLYYSNQHQAAGDVMAIHFAVYLASAALLGVFGDLQQQQEKMHWAPRLIIGFFIG
                     LVVLNAMFLSISTRGLPDKIASFLLPNPTKERVHTAFPGVIPHDRNKSYQPHLQRIEQ
                     QRNLGWKAELTGLDSLQNDKSSKLVLTITDKQQQPVTGAKIDLGFWRLANSHDDKHLM
                     MQETAAGRYEIDVTLPDAGRWVLELYVTKGEANFVKQQQIFVEE"
     gene            4020..>4404
                     /locus_tag="VN084_05370"
     CDS             4020..>4404
                     /locus_tag="VN084_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HWT28920.1"
                     /translation="MSSTPDQQTDPASANDCYHCGLPVPAGTRFRLRILDESRDLCCQ
                     GCLAVAGSIVDNGLEDYYRYRTELPQTADELVPDELRQLALYDHPEVQKSFVYTADDL
                     LREASLILEGITCAACIWLNERHLKQ"
BASE COUNT          953 a         1403 c         1202 g          846 t
ORIGIN      
        1 ctggcggctg aatccaagta cgaccatccg ttccagtggg gttccaagcg gaccggcccg
       61 gaccttgccc gtgtcggcgg aaagtactcc aacgagtggc atacccagca cttgaccgcg
      121 ccacgctcgc ttgtgccgca atcggtcatg ccgaactatc catggctctc ggccacgccg
      181 ctcgattatt ccgatatcga gaagcgcatg ctggcgctgc gcacaacggg cgtgccttac
      241 tccaagacca acgccgagta cgagcgtaac gtcaaggaat tcggcgaagt cgtggcgaag
      301 tcgctgcaca tcccggacgc cgagaagaac ttgatcgccc aggcgcaatt gggtaactac
      361 gataccaacc cggagaacat caccgagatg gatgcgctgg tcgcctacct gcagatgctg
      421 ggtacgctgg tcgacttcaa gaaatacgac gacgattact tcgtcaaatt ccgctagcac
      481 gaccgatagc aggaacggat aaggacgatc accatggaat ggctgctttg gttcaccaag
      541 ttcgagaaca ccaagccggt gtccctggtc atcttctttg tcgtgttctg cggggttctg
      601 gtttacttat acggcaacaa gaagcgcggc aagcagcttg aggattacaa gaacatcccg
      661 ctgcgcgacg acgagaaata aggcaaggaa aagcaattat ggctcagcaa aaggcaaaag
      721 gacctaccac gaccggacac gtctgggacg gtgacctgca ggaattgacc aacccgctac
      781 cgagatggtg gatctggggt ttctacctca cgttcatctt caccatcacc tattggttgc
      841 tctatcccgc ctggccgatc ggcggcaatt acaccaaggg cgtgcctggc ctcaacagca
      901 tcacctacac ggcgaccacg gtcgacggca aggaggtgca gaagaccacg cactggaaca
      961 tgcgttccaa gttcatggtc gagatgaacg agttgcatgc agcgcagaag caatggttcg
     1021 acaaggtggc tgccacgcca tatgccgatg tcgcccagga tgctgacctt atgcagttcg
     1081 tcaattcagc cggcaagacc ctgttctccg acaactgcgc accttgccac caggccggcg
     1141 gccagggcaa gatcgggttc tcgcctaacc tgacggatga ccactggcaa tatggcggta
     1201 cctacgaaga gatcaaccag accatcgtcg gtgggcgcca tggttacatg ccgcctttca
     1261 aggaagtcct ggacgacgcg cagatcaccc agctcgccaa ctacgtgctg agcctgtctg
     1321 gcgagcctaa cgattcggct gccgcgagtg caggcgaagc gctgttccac agcgagactg
     1381 ccgcctgcta ctactgccat ggcgccgacg ccaagggccg taccgtattg ggctcggcca
     1441 acctgaccga caagatctgg ctgtgggctg acatcccctc cgaaaagacg gcacaagcca
     1501 aggtcgacgc cgtgaagaaa gtgatcaccg gcggcctggc caaaggcgtg atgccgaact
     1561 ggaacgaacg tctcaaacca gaacaaatca agctgctgac cgtgtatgtg catgacggtc
     1621 tcggcggcgg taagtaagac gttaccccgg tccatccacg aaggctcacg cgacagcgtg
     1681 ggcctttatt ccatcgagcc atgctcgaag ccattgtttt ctaggacgat catctccaag
     1741 taggtcatat ggaagccagc agcgcaaagc agtcaccgct ctaccagaag cacatcccga
     1801 tcttcactcg ctcggtgaag ggccggttcc gtaacttcaa gacggccgtg atggtgctgg
     1861 cttacacgat ctatttcctg ctgccgtgga tgccctggca gcgtgtcagc gccgctgacc
     1921 aggccgtgct gttcgacctt ggttcacgcc gcttcttcat cttcgatctc atcgtctatc
     1981 cgcaggatat cttctggctg gcgatgctgc tgttcatcgc cgcggccttg ctgttcttcg
     2041 ccaccggcct gatcggccga gcctggtgcg ggtacttctg tttccagacg ctgtggtcgg
     2101 atctcttcat cctcatcgag aagaccatcc agggtgaacg cccagcccgc atgcggctct
     2161 acaaccagcc atggaacatg gagaagatcc tcaaggtcgg cggctcgcat gcgctgatga
     2221 tcctggtgtc gttctggacc gccgtcacct tcgcggccta tttcgcctac gcgccggatt
     2281 tcgtccgcga cctcttcacc ggccatgcgg ccgatgccgg ctacatcacc gtcatcgcac
     2341 tgacactgac cacgtatttc gctgccggca tcatccgcga acatatttgt accctggttt
     2401 gcccctacgc acgcttccag agcgtgatgt acgaagccga tacgctggcc gtgagttacg
     2461 acgcacgtcg cggcgaaggc acggccggcc gcgcgctacc caacgccggc atgaagaagc
     2521 gcgaagaccg ccaggccaag ggacacggcg actgtatcga ttgcggttac tgcgtgcagg
     2581 tatgcccgac cggcatcgac atccgtaacg gtctgcaata ccagtgcatc tcctgcggcc
     2641 tctgcatcga tgcttgcaac aacatcatgg attcggtcgg ctatccgcgt ggcctcatcc
     2701 gctacgattc cgaaaacaac ctgcatagcg aacaaccgca cgcgccgcgg ctggaatgga
     2761 aacgcctcaa ggtcatcggc tacgccgcgg cgttgatcat cgccaccggt gtgctgttct
     2821 acaacatcgg cacacgcgcc gctaccgaag ccaatgtcca gcagatccgc cagccactgt
     2881 tcatcatgct gtccgacggc agtatccgta accgctacca gatccatatc gtcaacaagt
     2941 cggagcagga cgaggtctac acgttgaccg tgcgcggcat ccctcaagac gcgctggaca
     3001 tcggcagtat cccagagatc aaggtgcgcg ccggcaagaa cctcaacctc aatgtgaagg
     3061 tcaatctgag cagtgaactt gccaagacca ccaatcgctt ccagttcgtc gtgacgccgt
     3121 ccggtgccga taggcaaccg atcgtgctcg acaccaactt ctacaacaaa caccagaatc
     3181 catgagcctg cccatcctcc ccatgaccga aaccctgttc ggcggcatcc tgctggcggc
     3241 ggtgttcttt ttcatcacgc gccgcgcggg cctgtccaat ttctgggcgg ctgtgctgag
     3301 tggggtgttg cccttccttg cgtatcttta ttacagcaac cagcaccagg ccgcaggcga
     3361 cgtcatggcc atccatttcg ccgtctatct ggcgagcgcg gccctgctgg gcgtgttcgg
     3421 cgatctgcag cagcaacagg agaagatgca ctgggcaccg cgcctcatca tcggcttctt
     3481 catcgggctg gtcgtgctca atgccatgtt cctgtcgatc tcgacgcgcg gcctgccgga
     3541 caagatcgcc agtttcctgc tgcccaaccc gaccaaggag cgcgtgcata ccgcctttcc
     3601 cggcgttatc ccgcatgatc gcaacaagag ctatcaaccc cacctgcaac gcatcgaaca
     3661 gcagcgcaac ctgggctgga aagccgaact gacggggcta gacagcttgc aaaacgacaa
     3721 gtcgagcaag ctggtgctga ccatcaccga caagcagcaa cagccggtca ccggcgccaa
     3781 gatcgacctc ggcttctggc ggctcgccaa cagccacgac gacaagcacc tgatgatgca
     3841 ggaaaccgcc gccggtcgtt acgagatcga cgtgacactg cccgatgccg gccgctgggt
     3901 gcttgagctc tacgtgacca aaggcgaggc gaatttcgtc aagcaacagc agatattcgt
     3961 ggaagagtaa gcgcgttctg cagcccgtgt cctgaattcg gccatgcatc ctgcccagta
     4021 tgtcatcaac ccctgaccaa cagaccgatc ccgcatccgc caacgattgc tatcactgcg
     4081 gattgccggt gcccgctggc acccgtttcc gcctgcgtat cctcgacgaa tcgcgagacc
     4141 tttgctgcca gggctgcctg gccgtagccg gctccatcgt cgataacggt ctcgaagact
     4201 actatcgcta ccgcaccgaa ctgccgcaga ccgcagacga gctggtgccg gatgaactgc
     4261 gccagcttgc gctctacgac cacccggaag tgcaaaagag cttcgtctac acggcagatg
     4321 acctgttgcg cgaggcctcg ctgatcctcg aaggcatcac ctgcgccgcc tgtatctggc
     4381 tcaatgaacg tcatctcaag cagc
//
LOCUS       DATMTW010000273         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_24251, whole genome shotgun sequence.
ACCESSION   DATMTW010000273 DATMTW010000000
VERSION     DATMTW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24251"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="VN084_05375"
     CDS             <1..1686
                     /locus_tag="VN084_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HWT28921.1"
                     /translation="DQLLWLSGYIAALGKHTVPVIADNGAPKPEASKVLVAYGTETGN
                     SQALASKLAFALEQKHIAFELKNLSQMRARHLSRYHFILIICSTHGDGDPPEPMSNFY
                     TSVMAADAPRMSGVHYAVLALGDSSYPKFCATGQALDDRLSALGAGRLLFRQDCDTDF
                     SDLASQWISDVSGLLAAKLPKAEASALARTDAKRPADSQATEHNRNHPLKAEVIENIR
                     LSHADRSDAIHHLELGIDTRLPELSSGDALGILVENAPRRARELIELAGCAEDAVVRV
                     NHRNIPLLQALIEECDITVPSKRLIVEWAKHSKNPLLRDLLVQDPQALREFLKAHQVC
                     DIAAGFPAVIDAQSLVDGLKPLQPRLYDLANSPRQSPEELHILVKRYQYAFNDRLCDG
                     VGSEYLAGLNAGDAVRVFPYRNAKFHFPQTEDTPVIFIAEGTGIGPFRAYLQDVAERA
                     HGPAWLIFAERSFEEDFLYQAEWQQAVADGQLALVDGIFHDRCDDACTIEALLHARQA
                     TLCEYLGQGAHVYLCGDRALLQGVEASLEALVSESAHPWAALQEAARIHRNLY"
     gene            1697..>3170
                     /locus_tag="VN084_05380"
     CDS             1697..>3170
                     /locus_tag="VN084_05380"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HWT28922.1"
                     /translation="MNSPKPSFNIDTPLEQLHANERLKWHSRHLRGDIATGLRNPLTG
                     AVPGEDPLLMKFHGIYQQDDRDLRASRQQRKLEPAYQFMVRLRLPGGTMTATQWLGLD
                     RIARNFAERGLRITTRQTLQLHGVRKRQLKRCIKALAAIGLDTISACGDDNRGVVCGA
                     NPHVSEAHAQVQQDAVAASERFKPKTHGYREIWYDAPLNQPADEEPFYRELYMPRKFK
                     IGFVLPPINDIDVYGQDLGFIAIVEDAHLVGYNVAVGGGMGKVDNREDSYPRLATVIG
                     FVERAQALDLAEVIMGIQRDHGNRKDRHQARFKYTLDRLGMPWFRETLAERWGRPLAA
                     ARTFSFRHNGDEPGWVQGDDGLWHGTLMIQSGRVRHALQDRLAAFFQHYDGEIRLTTN
                     QNLQLIKVPGYSLAQVKWRLDDLSLSDLLAPDTQAGFTLACVALPTCGLAMAEAERYL
                     PAFLARFNAIKAAHQIEAQPITLRITGCPNGCARPYLAEVA"
BASE COUNT          618 a         1088 c          951 g          513 t
ORIGIN      
        1 gaccagctgc tgtggctgag cggctatatc gcggcgctcg gcaagcatac cgtgcccgtt
       61 atcgccgaca acggcgcacc caaaccggaa gcgagcaagg tgctggtcgc ctacggcacc
      121 gagaccggta actcgcaagc gcttgccagc aagctcgcct tcgcgctgga acagaaacac
      181 atcgccttcg aactgaaaaa cctgtcgcag atgcgcgcac gccacttgtc gcgctatcac
      241 ttcatcctca tcatttgcag cacgcatggc gacggcgacc cgcccgagcc catgagcaac
      301 ttctacacct cggtgatggc ggccgatgcc ccgaggatga gcggcgtgca ttatgccgtg
      361 ctcgccctag gcgacagcag ctacccgaag ttctgcgcca ccggccaggc gctcgatgat
      421 cgcctgtcgg cactgggtgc cggccgcctg ctgttccgcc aggactgcga caccgacttc
      481 agcgatctag ccagccagtg gatcagcgac gtatccggcc tgctcgccgc caagctgccg
      541 aaggccgaag cgtcggccct ggcgcgcacg gacgccaagc gccctgccga tagccaggcc
      601 accgaacaca accgcaacca cccgctcaag gcagaggtga tcgaaaacat ccggctctcg
      661 cacgcggacc gcagcgacgc catccatcac ctcgagctgg gtatcgacac ccgcttgccc
      721 gagctatcca gcggcgatgc gctcggcatc ctggtcgaga atgcgccgcg ccgcgcacgc
      781 gaactgatcg aacttgcagg ctgtgccgag gacgccgtgg tgcgcgtcaa ccacaggaac
      841 atccccttgt tgcaggcact gatcgaggaa tgcgacatca ccgtgccgag caagcgcctg
      901 atcgtcgaat gggccaagca cagcaagaac cccttgctgc gagatctgct ggtacaggac
      961 ccgcaggccc tgcgtgaatt cctcaaggcc caccaggtct gcgatatcgc ggcagggttc
     1021 ccggcggtga tcgacgccca gtcgctggtc gatggcctga aacccctgca gccacggctc
     1081 tacgacctgg ccaatagccc gcgacaaagc ccggaagagc tgcatatcct ggtcaagcgc
     1141 tatcagtatg cgttcaacga tcgcctctgc gacggcgtcg gctccgagta tctggccggc
     1201 ctcaatgccg gcgacgcggt gcgcgtgttc ccctacagga acgccaagtt ccacttcccg
     1261 cagaccgagg acacgccggt gatcttcatt gccgagggta ccggcatcgg gcctttccgc
     1321 gcctacttgc aggatgtcgc cgaacgcgcc cacggcccgg cctggctgat cttcgccgaa
     1381 cgcagcttcg aggaggattt cctctaccag gcagaatggc agcaggccgt tgccgatggc
     1441 cagctcgcac tggtcgacgg catcttccat gaccggtgcg acgacgcctg caccatcgaa
     1501 gcgctgctgc acgcgcgcca agcgaccctg tgcgaatacc tggggcaagg cgcgcatgtc
     1561 tacctctgcg gcgatcgtgc gctactgcaa ggtgtcgaag ccagcctgga agcactggtc
     1621 agcgagagcg cgcacccatg ggccgcactg caagaagcgg cgcgcatcca tcgcaacctt
     1681 tattgaagcg gtctccatga attcgcccaa accctcattc aacatcgaca cgccgctcga
     1741 gcagctgcac gccaatgaac gattgaaatg gcatagccgt catctgcgcg gggatatcgc
     1801 cactggtctg cgtaatccgc tgaccggcgc cgtccctggc gaagacccgc tactgatgaa
     1861 gttccacggc atctaccagc aggacgaccg cgacctgcgc gccagcaggc agcaacgcaa
     1921 gctggagccg gcctaccagt tcatggtgcg cttgcggctg cccggcggca ccatgacggc
     1981 aacacaatgg ctgggcctgg accggatcgc acgtaacttc gccgagcgcg ggttgcgcat
     2041 caccacgcgc cagacgctgc agttgcatgg cgtgcgcaag cgccagctca agcgttgcat
     2101 caaggcactg gccgctatcg gcctcgatac catctctgcc tgcggcgacg ataaccgcgg
     2161 cgtggtctgc ggcgccaatc cgcatgtctc tgaagctcat gcccaggtcc agcaggacgc
     2221 ggtagcagcg agcgaacgct tcaaaccgaa gacgcacggc taccgcgaga tctggtacga
     2281 cgcgccgctc aaccagcctg ctgacgagga gccgttctac cgcgagctct acatgccgcg
     2341 aaaattcaag atcggtttcg tattgccgcc gatcaatgat atcgacgtct acggccagga
     2401 cctgggtttc atcgccatcg tcgaagacgc ccatctggtc ggctacaacg tcgcggtcgg
     2461 cggcggcatg ggcaaggtcg acaaccggga ggacagctac cccaggctgg ccacggtcat
     2521 cggctttgtc gagcgcgcgc aagcactgga tctggccgaa gtcatcatgg gcatccagcg
     2581 cgaccacggt aaccgcaagg accgccacca ggcacgcttc aagtacacgc tcgatcgcct
     2641 tggcatgccc tggttcaggg agacgctcgc ggagcgctgg ggtaggccgc tggcagcggc
     2701 aagaaccttc agcttccgcc ataacggcga cgagccgggc tgggtacagg gtgacgatgg
     2761 cctctggcat ggcacgctga tgatccagag cggccgcgtg cggcatgcgc tgcaggatcg
     2821 cctggctgcc ttcttccagc attacgatgg cgagatccgc ctcaccacca accagaacct
     2881 gcagctgatc aaggtgcccg gctattcgct ggcacaggtc aagtggcggc tggatgacct
     2941 ctcgttgagc gatctgctgg caccggatac ccaggccggg ttcacactgg cctgcgtcgc
     3001 cctgccgacc tgcggcctgg cgatggccga agccgagcgc tacctgcctg cgttcctggc
     3061 gcgattcaac gccatcaagg ccgcacacca gatcgaggcc cagcccatca cgctgcgcat
     3121 caccggctgc cccaacggtt gcgcccggcc ctatctggct gaggtggcgc
//
LOCUS       DATMTW010000274         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_258727, whole genome shotgun sequence.
ACCESSION   DATMTW010000274 DATMTW010000000
VERSION     DATMTW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258727"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VN084_05385"
     CDS             <1..601
                     /locus_tag="VN084_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWT28923.1"
                     /translation="IGQGEDGPTQQPVENTSGLRYIPNMQVWRPGSATETAVSWVAAA
                     ERKDGPTSLVLSRQNLPGIKHDTKHFDLIRKGGYVFSDVDGKADVIIIANGSELDLAV
                     KAAAELNAAGTKVRVVSMPSTNVFDKQDQAYRDSVLTPGVKRVAVEAAHPDFWRKYVG
                     LEGAVVGIDTFGESAPGGVLMKHFGFTVENVVKTVKSIL"
     gene            767..>3260
                     /gene="pepN"
                     /locus_tag="VN084_05390"
     CDS             767..>3260
                     /gene="pepN"
                     /locus_tag="VN084_05390"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWT28924.1"
                     /translation="MRTENAVKPRTIYLKDYTPAPYIASHVDLAFQLFEDKTVVTSTV
                     EYRKNPAGSSDTLVLNGEGQHIVSVLRDGQPFNDYTLDDGRMTVANAGARFTLTITAE
                     IDPAANTALEGLYKSQGNYCTQCEAEGFRRITFYQDRPDVLSTFHVRIEADKAAYPVL
                     LSNGNLVDAGDLPQGRHFTAWHDPFPKPCYLFALVAGKLVRIEDSFTTMSGRKVALHI
                     YVRPGDEAQCDHAMQSLKNAMRWDEEKYGREYQLDLFNIVAVSDFNMGAMENTSLNIF
                     NTALVLAHQETATDSDFLSVEGVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQE
                     FSADLNSRAVQRIDDVNTLRRLQFPEDGSPLAHAVQPDSFIEISNFYTKTVYDKGAEL
                     IRMQCTLLGEAAYRKATDLYFSRYDGHAVTCDDFVQCMADVSGRDMAQFFRWYKQAGT
                     PELRVVADYDATQQRYTLRFSQSQPDTPGQTGKLPLHIPIAVGLLDAQGRETHPTQVL
                     EMTEREQAFHFERVASRPVPSILRDFSAPVKLTTDLSDDDLRLLQLYDTDGFNKWEAG
                     QILALRNIARVMANPEADISTFIADVGVLIAQGLAGEGDKALLARALSLPSIAVIGQS
                     QSVVDPALIDRARTHILVSIKRAHKEALAALYDANANTGHFSITPDAMGRRALQNTVL
                     ELLTVTNGTGCAAWAKAHYLAADNMTDRVAALACLSDSTQPERDTVFADFYARFKDYP
                     LVVDKWFSLQATANRDAIFEDFAALRQHAEFNIKNPNRVRSLYTAFAINNPVRFHDPS
                     GQGYALLRDVIIELNALNPQMASRLVMPFREWK"
BASE COUNT          688 a         1007 c          958 g          607 t
ORIGIN      
        1 catcggccag ggcgaagacg gcccgaccca acagccagtg gaaaacacct ccggtctgcg
       61 ttatatccct aacatgcaag tatggcgccc aggtagcgcg accgagaccg ccgtgtcctg
      121 ggtcgctgcc gctgagcgca aggatggccc gaccagcctg gtcctgtccc gtcagaacct
      181 gccaggcatc aagcacgaca ccaagcattt cgacctgatc cgcaagggtg gttacgtgtt
      241 ctccgatgtc gacggcaagg ccgatgtcat catcatcgcc aacggttccg agctggatct
      301 ggcagtcaag gctgcagctg aactgaatgc tgccggcacc aaggtgcgcg tggtctccat
      361 gccttccacc aacgtgttcg acaagcagga tcaagcttac cgcgacagcg tactgacccc
      421 gggcgtgaag cgtgtggcag ttgaagccgc gcatcctgac ttctggcgca agtatgtcgg
      481 tctggaaggt gcagtcgtcg gtatcgatac cttcggcgaa tccgcgccag gcggcgtgct
      541 gatgaagcac ttcggcttca ccgtcgaaaa cgtcgtcaag accgtcaagt ccatcctgta
      601 aagatcgacc agatcgtagt gcagatcagg ccggatgcaa gtccggctcg atcgaaagaa
      661 gcgcctccca gtgaggcgct tttttttatg gctggacgga taccgctgct gctatgggtg
      721 agccaagtat aatgacagga aacccatcag catcaggcca cgtatcatgc gcacagaaaa
      781 tgcagtcaaa cccagaacga tctacctcaa ggactacacg cccgctccct acatagcctc
      841 acatgtggac ttggcattcc agttgttcga ggacaagacg gtcgtcacgt ccactgtcga
      901 ataccggaag aacccagctg gcagtagcga cacgctggtg ctgaacggcg aaggtcagca
      961 tatcgtcagc gtgctgcggg acggccagcc attcaatgat tacacgctcg acgacggcag
     1021 gatgaccgta gcgaacgcgg gagcgcggtt cacgctcacc atcaccgccg agatcgaccc
     1081 cgcagccaat actgcgctgg agggcctata caaatcgcag ggtaactatt gcacccagtg
     1141 cgaggccgag ggcttccgcc gcatcacgtt ctaccaggat cgcccggatg tactgagcac
     1201 cttccatgtg cgtatcgagg cagacaaggc ggcttatccg gtgctgcttt ccaacggcaa
     1261 tctggttgat gcaggcgatc tgccacaggg gcggcatttc acggcatggc atgacccgtt
     1321 ccccaagcct tgctacctgt tcgcgctggt ggcgggcaag ctggtgcgta tcgaggatag
     1381 cttcaccacg atgtcgggcc gcaaggtcgc gctgcacatc tatgtgcgcc cgggtgacga
     1441 agcgcaatgc gaccacgcca tgcagtcgct gaaaaacgcc atgcgctggg acgaggaaaa
     1501 gtacgggcgg gaatatcagc tcgacctgtt caatatcgtc gcggtcagcg atttcaacat
     1561 gggggcgatg gagaatacct cgcttaacat cttcaacacg gcgctggtgc tggcacacca
     1621 ggagacggcc accgatagcg atttcctcag cgtcgagggg gtcgtggcac atgagtattt
     1681 ccataactgg acgggtaatc gcgtcacttg tcgtgactgg ttccagctct cgctgaagga
     1741 ggggctgacc gtgttccgtg accaggaatt cagcgcagac ctcaattcgc gcgcggtgca
     1801 gcgcatcgac gacgtcaata ccttgcgccg cctgcagttc ccggaggacg gcagcccgct
     1861 ggcccacgcc gtgcagccgg acagcttcat cgagatcagt aacttctaca ccaagaccgt
     1921 ctacgacaag ggcgccgaac tcatccgcat gcaatgcacg ctacttggcg aagcggccta
     1981 ccgcaaggcg acggacctgt atttttcccg ctatgacggg catgccgtga cttgcgacga
     2041 cttcgtgcaa tgcatggccg atgtttccgg gcgtgacatg gcgcagttct tccgctggta
     2101 caaacaggcg ggtacgcccg aattgcgcgt cgtcgccgat tacgatgcaa cgcagcaacg
     2161 ctacaccctg cgtttcagcc aatcgcagcc ggatacgccg ggacaaaccg gcaagctgcc
     2221 gttgcatatc ccgatcgctg tcggcctgct ggacgcgcag gggcgcgaga cgcacccgac
     2281 gcaggtgctg gagatgaccg agcgcgaaca ggccttccac ttcgagcgcg tggcatcgcg
     2341 gcccgtgccc tccatcctgc gtgacttctc ggcgcccgtg aaattgacca ccgatctcag
     2401 tgatgacgac ctgcgcctgc tgcaactgta cgacaccgac ggtttcaata agtgggaggc
     2461 cggacagata ctggccctgc gcaatatcgc gcgcgtgatg gcgaatccgg aggccgatat
     2521 cagcaccttc atcgccgatg taggcgtgct gatcgcacaa gggcttgcgg gtgagggcga
     2581 caaggcgctg ctggcgcgcg cgctcagcct gccttcgatc gccgtgatcg ggcaatcgca
     2641 gtccgtggtc gacccggccc tcatcgatcg ggcgcgcacg catatcctcg tctccatcaa
     2701 acgcgctcac aaggaagcgc tggcggcact atacgatgcg aacgccaata ccggtcattt
     2761 ctctatcacg ccggacgcca tggggcgtag ggccttgcag aacacggtgc tggagctgct
     2821 gaccgtgacc aacggcacgg gctgcgcggc atgggccaag gcgcactacc tggcggcgga
     2881 taatatgacc gaccgcgtgg ccgcgctggc ttgcctgtca gacagcactc aaccggaacg
     2941 ggatactgtg tttgccgatt tttacgcccg gttcaaggat tacccgctgg tggtggacaa
     3001 atggttctcg ctgcaggcga cggcgaatcg cgacgcgatc ttcgaagact ttgctgctct
     3061 gcgccagcac gccgagttca atatcaagaa ccccaatcgc gtacgctcac tgtataccgc
     3121 gttcgccatc aacaacccgg ttcgattcca cgatcccagc ggacagggtt acgccctgct
     3181 gcgcgacgtc atcatcgagc tcaacgccct caatccacag atggcatcgc gtctggtgat
     3241 gcccttccgc gaatggaaac
//
LOCUS       DATMTW010000275         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_226058, whole genome shotgun sequence.
ACCESSION   DATMTW010000275 DATMTW010000000
VERSION     DATMTW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226058"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1137)
                     /gene="hemH"
                     /locus_tag="VN084_05395"
     CDS             complement(31..1137)
                     /gene="hemH"
                     /locus_tag="VN084_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWT28925.1"
                     /translation="MAYYHQEPPYQHGDQPRVGILLANLGTPDAPTAKALRPYLKQFL
                     SDRRVVEIPRLIWWCILNLIILNLRPRKSAEKYAQIWSSEGSPLLAHAQKQAKLLQGF
                     LGTRIASPFQVELGMRYGNPSIESAIDKLKAQHCDRILVFPLYPQYAASSSASALDAV
                     WQKLLRMRNVPAIRSIRHYHDHPAYIEALAQSVRAHWAEHSRPDKLIMSFHGVPKFSL
                     LKGDPYHCECHKTGRLLAEALGLTKDQYMVCFQSRFGKAEWLKPYLAPTLEALGKQHL
                     KRVDVICPGFSSDCLETLEEIAMEGKHLFTAAGGGDYHYIPALNEQEAWIHAMCDIAL
                     ENLQGWVSVSWDRQAAAEAAELTSLRAKALGAQA"
     gene            complement(1176..2195)
                     /gene="hrcA"
                     /locus_tag="VN084_05400"
     CDS             complement(1176..2195)
                     /gene="hrcA"
                     /locus_tag="VN084_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWT28926.1"
                     /translation="MLDKRAQILLKTLVEHYISDGQPVGSRTLSKCSGLDLSPATIRN
                     VMSDLEELGFIASPHTSAGRIPTQRGYRFFVDTLLTIQPLQSQEIQQFQQQFSSPDPQ
                     ELIASAAGMLSNLTQFAGVVMVPRRKSVSFRHLEFLPLSEKRILVIIVTSDGNVQNRI
                     IITDKPYSASELTQASNFFNQHYAGSTFEQVQKKLHEELKQMQSDMTRLMTAAMEAST
                     KVLNEDRDNVVISGERNLLQIDDLSTNVTSLRKLFEVFERRTSLMQLLDNSQRADGIQ
                     IFIGGESGYLPLDECSMVTAPYEANGQTVGTLGVIGPTRMAYERVIPIVDVTAKLLSN
                     ALNNS"
     gene            2413..>2832
                     /locus_tag="VN084_05405"
     CDS             2413..>2832
                     /locus_tag="VN084_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWT28927.1"
                     /translation="MKSTFKTVALIGKYMHPQMRDQIVALAQFLTGRQHAVLIEEKTA
                     ENSGIGGYTTVHVNAIGMHADLAIVVGGDGTMLTAARALVDHKIPLVGVNRGRFGFLT
                     DLSSESMIEGLGRILDGAYDSEQRILLSASITRQGKVI"
BASE COUNT          524 a          798 c          896 g          614 t
ORIGIN      
        1 accgccgttc cgcctctgga tttacctgtt tcaggcctgt gcgcccaagg ccttggcgcg
       61 caggctggtc agctcggcgg cctctgctgc cgcttgcctg tcccaggata cgctcaccca
      121 gccctgcagg ttctccagcg cgatgtcgca catggcatgg atccaggctt cctgctcgtt
      181 gagtgccggg atgtaatggt aatcgccgcc gccggccgcg gtgaacaggt gcttgccttc
      241 catggcgatc tcctccagcg tttccaggca atcgctggag aatccggggc agatgacgtc
      301 gacgcgcttc aggtgctgct tgcccagcgc ttccagcgtg ggcgccaggt agggcttgag
      361 ccattcggct ttgccgaaac gggattggaa gcacaccatg tattgatcct tggtcagccc
      421 cagcgcctcg gcgagtaacc tgccggtctt gtgacattcg caatggtatg ggtcgccctt
      481 gagcaggctg aacttgggca cgccgtggaa gctcatgatc agtttgtccg gccggctgtg
      541 ctcagcccag tgcgccctga cgctttgcgc cagagcttca atgtaggccg ggtggtcatg
      601 ataatgacgg atgctgcgga tggccggcac attgcgcata cgcagcaact tctgccatac
      661 cgcatcgagc gctgaagcgc tgctgctggc cgcatattgc ggatacaggg ggaacaccag
      721 gatacggtcg caatgctgcg ccttaagctt gtcgatggcg gattcgatcg aggggttgcc
      781 gtaacgcata ccgagttcga cctggaaagg cgatgcgatg cgcgtgccga ggaacccctg
      841 cagcagcttg gcctgctttt gtgcatgcgc cagcaatggc gaaccttcac tggaccagat
      901 ctgcgcatac ttctccgcag atttgcgggg gcgcaggttg aggatgatga gattgaggat
      961 gcaccaccag atcagccgcg ggatctcgac tacgcggcga tcgctgagaa actgcttgag
     1021 atagggacgc aaggccttgg cagttggcgc gtcgggcgtg ccgagattgg ccagcaatat
     1081 gcccacgcgg ggctggtcgc catgttgata gggcggttcc tgatggtaat aggccatggt
     1141 tcttctttga aagtggattg gcggtgcgag atgcttcagc tgttattgag cgcgttggac
     1201 aacagttttg cggtgacgtc tacgatgggg atgacccgct cgtaagccat gcgtgtcggg
     1261 ccgatcacgc ccagcgtgcc gacggtctgg ccgttggctt cgtagggcgc agtgaccatg
     1321 ctgcattcgt ccagcggcag atagccggat tccccgccga tgaagatctg gataccgtcg
     1381 gcgcgctggc tgttgtcgag gagttgcatg agtgacgtcc ggcgctcgaa gacttcgaac
     1441 agcttgcgca aggaagtcac gttggtcgaa agatcgtcga tctgcagcag gttgcgttcg
     1501 cccgagatga cgacattgtc cctgtcctcg ttcagcacct tggtgctggc ctccatggcg
     1561 gcagtcatca ggcgtgtcat gtccgattgc atctgcttca gctcttcatg cagcttcttc
     1621 tggacttgct cgaaagtgct gccagcatag tgctggttga agaaattgct tgcctgcgtc
     1681 agttcggaag cggagtaggg cttgtcggtg atgatgatgc gattctgcac attgccgtcg
     1741 gacgtgacga tgatgaccag gatgcgcttt tccgatagcg gcaggaattc gaggtgacgg
     1801 aaggagaccg atttgcggcg cggcaccatg acgacgccgg cgaattgggt caggttggac
     1861 agcatgccgg cggcagaggc gatcagttcc tgcgggtcgg gcgacgagaa ttgctgctgg
     1921 aattgctgga tctcttggct ctgcaatggc tggatcgtga gcaatgtgtc gacaaagaag
     1981 cgatagccgc gctgggtcgg gatacggccg gccgaggtgt gcgggctggc gatgaagccc
     2041 agctcctcca ggtccgacat gacgttgcgg atggtcgccg ggctcaggtc cagtccggaa
     2101 catttcgaaa gcgtgcgcga gccgaccggc tggccgtcac tgatgtagtg ttcgaccagg
     2161 gtcttgagca ggatctgtgc gcgtttatct agcatggcga tatggtgtgt agatgcgggc
     2221 cagaagctaa aacttcaagc tggcatccgg ctgcaaaatt atccggcagg cgccctgtat
     2281 gcgcaataca tattcttgcc cgcaagtgcg catgccggcg ccgctcccga tcgagccggc
     2341 catatcacat aggctgcgcc agccgagacc tggcgcttct atgccaacgt catctatgct
     2401 ttaatgccag ccatgaaaag cactttcaaa acggtcgccc tcatcggcaa atatatgcat
     2461 ccccagatgc gggaccagat cgttgccctg gcccagttcc tcactggtcg ccagcatgcc
     2521 gtcctgatcg aggagaagac ggcggagaac tcgggtatcg gcggctatac caccgtccat
     2581 gtcaatgcca tcggcatgca cgccgatctt gccatcgtag tcggtggcga cggcaccatg
     2641 ctgaccgcgg cccgtgcgct cgtcgatcac aagatcccgc tggtcggcgt caatcgtggc
     2701 aggttcggtt ttctcaccga cttgagctcc gaatccatga tcgaaggcct gggccgcatt
     2761 ctcgatggtg cctacgatag cgagcagcgc atcttgctct ccgccagcat cacccgccag
     2821 ggtaaagtca tc
//
LOCUS       DATMTW010000276         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_561533, whole genome shotgun sequence.
ACCESSION   DATMTW010000276 DATMTW010000000
VERSION     DATMTW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561533"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="ribD"
                     /locus_tag="VN084_05410"
     CDS             <1..967
                     /gene="ribD"
                     /locus_tag="VN084_05410"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWT28928.1"
                     /translation="GEGAHLRAGEHHAEVDALRQAGDLSRGADVYVTLEPCSHYGRTP
                     PCANALIQAGVKRVFAAMQDPNPDVAGSGLKLLEAAGIEVHAGLLQPEAEAMNPGFIS
                     RMTHGMPYVRSKIAASLDGRTALANGASQWITGEAARTDVQHWRARSCAILTGSATVR
                     HDDPQMTVRSLDIGRQPLRVVADSRLGISPHARILADGNALVAYASDPDGKAEILRAA
                     GVELVHLGDAQGRVCLSSLLRALAARGINEVLVEAGEGLNGALLQHDLIDECLFYYAP
                     VLMGSSAKGMFDIPALTEMAQRKHLEILDVRRIGPDLRVQARRQK"
     gene            984..>1697
                     /locus_tag="VN084_05415"
     CDS             984..>1697
                     /locus_tag="VN084_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWT28929.1"
                     /translation="MLIDTHCHLDAAEFDADRAAVAQDAPAHDVRLIVVPAVERGNFT
                     TVIQLADAYPHCAYALGIHPLYIERAVDDDLVQLRALAETHRPVAIGEIGLDYFVEGY
                     DGERQRHYFVEQLKIAQALALPVILHVRRSIDDILKYLRRHRPVGGIAHAFNGSRQQA
                     EEFIKLGFKLGFGGAITYPRALKIRELAATLPAESIVLETDAPDIAPEWLGHHGRNAP
                     AQLARIAQVLAELRGVDMAE"
BASE COUNT          307 a          532 c          551 g          307 t
ORIGIN      
        1 cggcgagggc gcgcacctgc gagccggcga gcatcacgcg gaagtcgacg ccttgcgaca
       61 ggcgggcgat ctctcccgtg gcgccgatgt ctatgtcacc ctcgaaccct gcagccatta
      121 cggccgcacg ccgccctgcg ccaacgcctt gatccaggcg ggcgtcaaac gcgtattcgc
      181 ggccatgcaa gaccccaatc ccgatgtcgc aggtagcggc ctcaaactgc tcgaggccgc
      241 cggtatcgaa gtccatgccg ggctgctaca gcctgaagcc gaagccatga acccgggctt
      301 catctcgcgc atgacacacg gcatgcctta cgtacgcagc aagatcgccg ccagtctcga
      361 tggccgtacg gcattggcga acggcgccag ccagtggatc acgggcgagg cggcgcgtac
      421 ggatgtgcaa cactggcgtg cccgctcctg tgccatcctc accggcagtg cgacggtgcg
      481 gcacgatgat ccgcagatga cggtgcgtag tctcgatatc ggtcgccagc ccttgcgcgt
      541 ggtggccgat agtcggctgg gcatctcgcc gcatgcgcgt atcctcgccg acggcaatgc
      601 attagtggcc tatgccagcg atcctgacgg caaggccgag attctgcggg cggccggcgt
      661 ggaactggtg catcttggtg atgcgcaagg tcgcgtctgc ctgtcgagtc tgctgcgcgc
      721 ccttgccgcg cggggcatca acgaggtgct ggtcgaagca ggcgagggcc tcaatggggc
      781 gctgctgcag cacgacctga tcgatgagtg tctcttctat tacgcgccgg tgctcatggg
      841 gagcagcgcc aaaggcatgt tcgacatccc tgcgctgacc gagatggcgc agcgcaaaca
      901 tctggagatc ctcgatgtcc gtcggatcgg tccggatctg cgcgtgcagg cgagacgaca
      961 gaaataggac gcggagccag cgcatgctga tcgataccca ctgccatctg gatgctgcgg
     1021 agttcgatgc cgaccgggcg gccgtagcgc aagacgcccc cgcgcatgac gtccgactga
     1081 tcgtcgtgcc agctgtcgag cgcggcaatt tcacgaccgt catccagctc gcggacgcat
     1141 atccgcactg tgcctatgcg ctgggaatcc atcctttata tatcgagcgc gcggtcgacg
     1201 acgatcttgt gcaattgcgc gcgctggccg agacgcaccg gccagtggcg atcggtgaga
     1261 tcggcctcga ttattttgtc gagggttatg atggcgagcg ccagcgtcat tacttcgtcg
     1321 aacagctgaa gatcgcccag gcgcttgcct tacccgtcat cctgcatgtg cgtagatcga
     1381 tcgacgatat cctcaagtat ctgcgacggc atcggccggt aggcggcatc gcgcacgcct
     1441 tcaacggcag ccgtcagcag gcggaggaat tcatcaagct cggtttcaag ctgggttttg
     1501 ggggtgccat tacctatccg cgggcgttga agatccgtga actggccgcc accctgccgg
     1561 ccgagagcat cgtgctggag accgacgccc ccgacatcgc ccctgaatgg ctggggcacc
     1621 atggccggaa tgcgccggca cagttggcaa ggatcgccca agtgctggca gaattgcggg
     1681 gtgtggatat ggcggaa
//
LOCUS       DATMTW010000277         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_292007, whole genome shotgun sequence.
ACCESSION   DATMTW010000277 DATMTW010000000
VERSION     DATMTW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292007"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..314)
                     /locus_tag="VN084_05420"
     CDS             complement(33..314)
                     /locus_tag="VN084_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWT28930.1"
                     /translation="MNKILVALLLSIGLSLNAYAAVDLNTASKAELESLKGVGPAKAD
                     AIIEYRKKSGGFKSVNDLDNVPGFGEKTVEGLKKEVTVGAAKSAAVQKK"
     gene            complement(411..1109)
                     /gene="pyrF"
                     /locus_tag="VN084_05425"
     CDS             complement(411..1109)
                     /gene="pyrF"
                     /locus_tag="VN084_05425"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWT28931.1"
                     /translation="MPDPKIVVALDYADAASALKLVDQLDPSLCRLKVGKELFTAAGP
                     RFVETLVAKDFGVFLDLKFHDIPNTVAKACEAASRLGVWMLNVHASGGLAMMQAAKEG
                     VARSGHSPLLIAVTVLTSMDQATLDQIGVAGSLQDQVLNLARLTQRAGLHGVVCSAQE
                     AAMLRQALGDTFCLVTPGIRPAEASLDDQNRVVTPAKALQMGSSYLVIGRPITQAPDP
                     LAALQAIQREISVN"
     gene            complement(1115..2278)
                     /gene="lapB"
                     /locus_tag="VN084_05430"
     CDS             complement(1115..2278)
                     /gene="lapB"
                     /locus_tag="VN084_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HWT28932.1"
                     /translation="MEFEYWWLLALPLFFTLGWIAARVDINQLLSESTTLPAAYFKGL
                     NFLITDQHDKAIEAFVEAVEANADSLELHFALGSLFRRRGEVDRAIHLHLNLLERREL
                     PEPQKMAVMAELAQDYLKAGLLDRAEELFKSLDDSRYKQPALRALLEIYVREREWERA
                     IESAKELERLSGVPFRKEIAQYYCEMAMKSILTNDHHTARHELEKALDANKGCVRANI
                     LLGDMEAADGAHKVAISMWKRVEFQKPEYLGLIAARLLTSYRAIGKLDEGMAQLRSYL
                     QTYKLPSLQAVVYEAVLAEQGPESAAVLAREELSKKPSLQTLDQLLKARMLFDKHDQQ
                     DTNLTQQTVRAAIGTRNAFVCDQCGFRAKNFHWQCPACNAWEAIAPEPNEPNA"
     gene            complement(2314..2607)
                     /locus_tag="VN084_05435"
     CDS             complement(2314..2607)
                     /locus_tag="VN084_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HWT28933.1"
                     /translation="MRYVYLTLVFLLFVLLLGFALKNAEIVELHYYLGFIWRAPLSLM
                     LLIAFFFGTVAGMIACLNPLIRQRRQLIAMQRELRQLDPHTQAAARGTTATGH"
BASE COUNT          487 a          739 c          842 g          583 t
ORIGIN      
        1 gcggggcttt ttgttgatct gatgcaagca gattatttct tctggactgc tgcggatttc
       61 gcggcaccga cagtcacttc cttcttgaga ccttcgacgg tcttttcacc gaagcccggt
      121 acgttgtcga ggtcgttcac cgacttgaag ccgccgctct tcttgcggta ctcgatgatg
      181 gcgtcagcct tggcaggccc tacgcccttc agcgattcga gttctgcctt ggaagcggtg
      241 ttgaggtcga cggccgcata ggcgttgagc gagaggccga tgctcagcag aagcgcgact
      301 aggatcttgt tcatattgtt ctcccattaa tagttttaga tgcaggccgt atctggcgct
      361 gcgtctgcat cttaacgacg ggagaggtgt tgcggaccct cgcagggttg ctagttgacg
      421 ctgatctcac gctggatcgc ctgcagggca gccagcgggt caggcgcttg cgtgatgggg
      481 cgtccgatga cgagataact cgatcccatc tgcaaggcct tggcgggtgt cacgacccga
      541 ttctggtcat caaggctggc ttcagctggg cgtatgccgg gtgtgaccag gcagaaggtg
      601 tcgccgagtg cctgacgcag catggcggct tcctgtgccg agcagactac gccgtgcagg
      661 ccggcgcgct gggtcaggcg ggcaagattg aggacctggt cctgcaggct gcccgcaacg
      721 cctatctggt cgagcgtggc ctggtccatg ctggtgagga ccgtgaccgc gatcagcagg
      781 ggcgaatggc cgctacgggc aaccccttct ttcgcggctt gcatcatcgc cagcccaccg
      841 ctggcatgca cgttgagcat ccagacgccg agccggctcg cggcctcgca agccttggca
      901 acggtattcg ggatgtcatg gaacttgaga tcgaggaaca cgccgaaatc tttcgcgacc
      961 agtgtttcga cgaatcgcgg gccagcggcg gtgaacagtt ccttgcccac cttgaggcgg
     1021 cataggctcg ggtcgagctg atcgaccagt ttcagggcgc tggcggcatc cgcgtaatcc
     1081 agcgcaacga cgattttcgg atcaggcatg gtcatcaggc gttcggttcg ttaggttctg
     1141 gcgcaatcgc ctcccaggcg ttgcaagccg ggcattgcca gtggaagttc ttggcgcgga
     1201 agccgcactg gtcgcagacg aaggcgttac gggtgccgat ggcggcgcgt accgtttgct
     1261 gcgtcagatt ggtatcttgc tggtcgtgct tatcgaacag catgcgggcc ttgagcagtt
     1321 ggtccagtgt ttgcaggctg ggtttcttgc tcagttcttc gcgagccagg acggcggcgc
     1381 tttcgggtcc ttgctcggcc aggacggctt catagacgac ggcctgcagg gacggcagct
     1441 tataagtctg caaatagctc cgcagctggg ccatgccttc atcgagtttg ccgatggcgc
     1501 ggtagctggt gagcaggcgc gcggcgatca gtccgagata ttccggcttc tggaattcca
     1561 cgcgcttcca catcgagatc gcgaccttgt gcgcaccgtc ggcggcttcc atgtcgccca
     1621 ataggatatt ggctcgtacg catcccttgt tcgcatcgag cgctttttcc agttcatggc
     1681 gcgcggtatg gtgatcgttg gtgaggatgg acttcatcgc catctcgcag tagtactgtg
     1741 cgatctcctt gcggaacggc acgccggaca ggcgttccag ctccttggcg ctctcgatgg
     1801 cgcgctccca ttcccgctca cgcacataga tctcgagcag ggcgcgcaag gcgggctgct
     1861 tgtagcggct atcatccagg gatttgaaca gctcttccgc gcgatcgagc aggccggctt
     1921 tcaaatagtc ctgcgccagt tctgccatca cggccatttt ttgcggctca ggcagctcgc
     1981 gtctttcgag caggttgagg tgcaggtgga tggcgcgatc gacttcgcca cgcctgcgga
     2041 acaggctacc gagcgcaaaa tgtagttcca gtgagtcggc gttggcttcg acggcctcga
     2101 caaaggcctc gatcgccttg tcgtgctgat ccgtgatgag gaagttgagc cccttgaagt
     2161 aagcggcagg cagggtcgtg gattccgaca acagctggtt gatgtcgaca cgtgcggcga
     2221 tccatcccag cgtgaaaaag agtggaagcg ccagtagcca ccagtattcg aattccatgg
     2281 ttatccgtct ttattgtttg agatgaatcg gcatcaatgg ccggtggcgg ttgtaccgcg
     2341 ggcggccgcc tgcgtgtgcg ggtcgagttg gcgcaattca cgctgcatcg cgatcaattg
     2401 gcgacgttgc cggatcagcg ggttgaggca ggcgatcatg ccggctacgg tgccaaagaa
     2461 gaaggcgatg agcagcatga gcgacaaagg cgctcgccag atgaaaccca ggtaatagtg
     2521 gagctcgacg atctccgcat ttttcaaggc gaatccgagc agcagcacga acagcaaaaa
     2581 caccaaggtc aggtaaacgt aacgcatggc ccgattctac ctaattcagg ggtgcaacgc
     2641 caaaatatgc c
//
LOCUS       DATMTW010000278         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_24426, whole genome shotgun sequence.
ACCESSION   DATMTW010000278 DATMTW010000000
VERSION     DATMTW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24426"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..514)
                     /locus_tag="VN084_05440"
     CDS             complement(56..514)
                     /locus_tag="VN084_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWT28934.1"
                     /translation="MRYWLMKSEPADVSIDDLAKRPNQTVDWYGVRNYQARNFMRDQM
                     KLGDGVLFYHSNCDEPGIVGIAEVSALAYPDRLQFIKGHKYHDPKATPENPRWFNVDV
                     KLVRKTRLLSLKEMRENPKLANLRILQRGNRLSITPVDPADWETIMALLA"
     gene            583..942
                     /locus_tag="VN084_05445"
     CDS             583..942
                     /locus_tag="VN084_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HWT28935.1"
                     /translation="MAKNVLGTTLQVCGLDPLTGFTRNGCCETGPEDTGSHTVCAQVT
                     EEFLAFSYSRGNDLSTPRPEYGFEGLAPGDRWCLCAARWQEAADAGLAPPVILEATHE
                     RALEIISLADLKYHALR"
     gene            complement(1186..1491)
                     /locus_tag="VN084_05450"
     CDS             complement(1186..1491)
                     /locus_tag="VN084_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWT28936.1"
                     /translation="MSDMQGVDVNIMGREFKVACSDDEREGLINAVNFLDRKMRDIRD
                     TGKVVGVERIAIMAALNLAHELLTTRSGGFDIGDFQRRIVAMQEQIDEAVAEQDNLF"
     gene            complement(1488..1688)
                     /locus_tag="VN084_05455"
     CDS             complement(1488..1688)
                     /locus_tag="VN084_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28937.1"
                     /translation="MEADLKALEDKLKQLIALCQSLREENLELRQDLAQAQDDVKQLR
                     DNMTEASVRLEALIQRLPEESV"
     regulatory      1804..2014
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2150..>2610
                     /locus_tag="VN084_05460"
     CDS             2150..>2610
                     /locus_tag="VN084_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWT28938.1"
                     /translation="MKRTALACLLGSLFSSTTHAAPLEFQTGDIVVTASRIPQPRESV
                     IGDTTVIEREEIQRAGAQTITDLLQAQPGIEINSNGGPGKVSGIFMRGTNTDHVVVLI
                     DGMRVNSATLGTTAFENIPLAQIDRIEILRGPASSLYGADAIGGVIQIFTR"
BASE COUNT          594 a          756 c          714 g          546 t
ORIGIN      
        1 gtttttcatt ggtgggtcac aagcgctcgt gcatgcgcca ggcctcgtga tcagatcagg
       61 ccagaagcgc catgatggtt tcccagtcgg caggatccac aggcgtgatc gacagacgat
      121 taccgcgctg caggatacgc agattggcga gcttcgggtt ctcgcgcatc tctttcaaac
      181 tcaacaggcg cgtctttcgc accagcttca catcgacatt gaaccagcgt ggattctccg
      241 gcgtcgcctt aggatcatgg tacttatgcc ccttgatgaa ctgcaggcga tcaggataag
      301 ccagcgccga cacctcggcg ataccgacaa taccaggctc atcacagttg gaatggtaga
      361 acagcacgcc atcgccgagc ttcatctggt cgcgcatgaa gttgcgcgct tggtaattac
      421 gcacgccata ccagtcgacg gtctgattcg gccttttcgc aagatcgtcg atcgagacgt
      481 cggcgggttc ggatttcatg agccagtaac gcattcttgt tctcgtggga tgggataatt
      541 tcgtatcata aacgggacga ccagaaaatc gaaggcaagc gcatggcaaa gaatgttctc
      601 ggcacgacac tgcaagtctg cggcttggac ccgctgaccg gcttcacacg caacggctgt
      661 tgcgagaccg gccccgaaga tacgggcagc catacggtct gcgcgcaggt gacggaagaa
      721 ttcctcgctt tttcgtattc acgtggcaat gacctcagca ctccgagacc ggagtacggc
      781 ttcgaagggc tggcaccagg cgaccgctgg tgcttatgcg cagcaaggtg gcaagaagcc
      841 gcggatgccg ggctggcgcc gccagtgatc ctggaggcga cccatgagcg cgcgcttgag
      901 atcatcagtc tagctgacct gaaataccac gcgctgaggt gagagatgtc cctgcaaaca
      961 tcacttaggc cagcatcctg aaccaaaaag gttcaagtgg taacggacta gagcacttca
     1021 ggcacacccg cgctttgaaa gctgagcgcg cacaccgcga ctcgattgac cgaaaccgat
     1081 gccagagact agttcaagga atatatagcc taagcaacat cgcagggaac aaaccggaaa
     1141 ccagcaactg aagatgacaa ctgccggctt taagaaactt tgaagtcaga acaaattgtc
     1201 ttgctcggct accgcctcgt caatctgttc ctgcatggct acgattctac gctggaaatc
     1261 cccgatgtca aagccgccgc tacgtgtggt gagcaactca tgcgcgaggt tgagtgccgc
     1321 catgatggcg atacgctcga ccccgacgac cttaccggta tcacggatat cgcgcatctt
     1381 gcgatcgagg aaattgaccg cgttgatcag gccttcgcgt tcgtcatcgc tgcaagcgac
     1441 cttgaattcg cggcccatga tattgacatc gacgccctgc atatcgctca tacggattcc
     1501 tctggcaagc gctggatcag cgcctcgagc cggacgctgg cctcggtcat gttatcccgc
     1561 agctgcttga cgtcatcctg tgcctgggca aggtcctggc gcagctcaag gttttcctcg
     1621 cgcaaggatt ggcatagcgc aatcaattgc ttgagcttgt cctccagggc tttcagatcg
     1681 gcttccatgg ctgcactata atttgaaatt ccctgtatgg tcaactgcta acagggattt
     1741 ttgaaagtaa tacagcatgt tatatgaaaa ataaatattc agcttcgact atgcataatc
     1801 tataatcgat gcaagttgtc aggtgcccgc atcgcatttc ttgcgttcgg gtgaaactgg
     1861 gaaacaggtg cgcttcatgg actggagcaa tgcctgtgct gcccccgcaa cggtaagcga
     1921 gtgaggttcc acattcagcc actgggcttc tgcctgggaa ggcgtggatc aagactcgtg
     1981 agcccggaga ccggcctgag aacccaagtt tggaataacc gcggggtgac ggttctgaca
     2041 cgcatgcatc gaggtcttcg atgcgccatg gtctctactt tcgttcccgg tatttccacg
     2101 atcgcaacca gaacatgcgg ggtcgcatgt gattaggaaa accagaacaa tgaaaagaac
     2161 cgccttggcg tgcctgctcg gctcgctatt ctcaagcacc acccacgctg cccctttgga
     2221 attccagacc ggtgacatcg tcgtcacggc gagccgtatt ccccaaccgc gggaatccgt
     2281 catcggcgac accaccgtca tcgagcgcga ggagatccag cgcgccggcg cccagaccat
     2341 caccgacctc ctgcaagccc agcccggcat cgagatcaac agtaatggcg gccccggtaa
     2401 agtctccggc atcttcatgc gcgggaccaa caccgatcat gtggtggtgc tgatcgacgg
     2461 catgcgcgtc aactcggcga cgcttggcac cacagccttt gagaacatcc cgctggccca
     2521 gatcgaccgc atcgagatcc tgcgtggccc agcctccagc ctgtacggtg cggacgcgat
     2581 tggcggcgtg atccagatct tcacgcgcaa
//
LOCUS       DATMTW010000279         5243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_325885, whole genome shotgun sequence.
ACCESSION   DATMTW010000279 DATMTW010000000
VERSION     DATMTW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325885"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VN084_05465"
     CDS             complement(<1..1028)
                     /locus_tag="VN084_05465"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWT28939.1"
                     /translation="MDIKQYMEGLGQQARLASRAMAKANANTKNLALLTIAKAIRRDQ
                     AQLLAANQADLEAARKNGLEPAMLDRLTLSEKSIATMAEGLEQIASLPDPIGEMSDFK
                     YRPSGIQVGRMRVPLGVIGIIYEARPNVTVDAAGLCIKSGNAAILRGGSEAIQCNRAL
                     AALVDEGLTAAGLPHTAVQVVETTDRAAVGELITMKHYVDVIVPRGGKGLIERISNEA
                     RIPVIKHLDGNCHIYVDDDADMAKALPILENSKTQRLGTCNTAESLLVARSIAADALP
                     KLASMLLAKGVEIRGCAETRALVKEAKAATDEDYYTEYLDAIISVKVVSGVDEAIAHI
                     NQYSSQHT"
     gene            complement(1065..2084)
                     /gene="holA"
                     /locus_tag="VN084_05470"
     CDS             complement(1065..2084)
                     /gene="holA"
                     /locus_tag="VN084_05470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWT28940.1"
                     /translation="MRLQQSQLQQHLAKELRPLYVLMGDEPLAQRECLDAIRATARQQ
                     GYDERTSLIVERNFNWQQVAAFGQAISLFSSRRILELNIPSGKPGTDGSKALQALAEQ
                     PMADTVVIVILPKLDRDGKNSAWYAALESAGVSLALLDVPTDQLPRWISDRLAVQGQH
                     TDADTLAFLAHQVEGNLLAAHQEIQKLGLLYPQGALDGQAVREAVLNVSRYDAFQLGD
                     AVLAGDAERTIRILQGLKDEGAQPVAVMNPLSWVIRPLARIKQAEANGENLANVMQQA
                     RIYGDRQALVRRAISRLSLKQLLAAQAKLSEIDRTAKGLLQGDAWLEISRLCFGLARI
                     QKRSR"
     gene            complement(2092..2586)
                     /gene="lptE"
                     /locus_tag="VN084_05475"
     CDS             complement(2092..2586)
                     /gene="lptE"
                     /locus_tag="VN084_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWT28941.1"
                     /translation="MSVKRLLHIILLLSLLALTACGFQMRGYADLSFRKLYINGNISI
                     SKDLKKSLAVNGVEVVTEKEDAELMMDLMNEVSEKRILSLSGTGVVREFDLIYKVTYR
                     IKQPDEDLWGEPKTIETRRDFSYSDREVLATGYEEQRLNAEMRQETTQQLVRSLVAYK
                     VTKK"
     gene            complement(2591..5221)
                     /gene="leuS"
                     /locus_tag="VN084_05480"
     CDS             complement(2591..5221)
                     /gene="leuS"
                     /locus_tag="VN084_05480"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWT28942.1"
                     /translation="MQQQYPFKELEQAVQQTWEQNQSFVAKPDSDKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYHRMKGYNVLQPMGWDAFGLPAENAAIQNKVPPAKWTY
                     DNIAYMKKQLKSLGFAIDWSRELATCQPEYYKWNQWLFLRMLEQGIAYKKTQVVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKREIPGYYLNITGYAQQLLDDLDKLPGWPERVKT
                     MQANWIGKSVGVRFAFTHDIKDTSGQLIDDGKLWVFTTRADTIMGVTFCAVAAEHPLA
                     TLAASSNPALAAFIEKCKQGSVMEADMATMEKEGMDTGLTVAHPLTGEQVPVWVGNYV
                     LMTYGEGAVMAVPAHDERDFEFAKKYSLPIKQSIAIEGEEFSLAGWQEWYGDKTRGTC
                     THSGKYDGLAYEAAVDAIAADLKAKDLGDKQINWRLRDWGISRQRYWGCPIPIIHCER
                     CGDVPVPDDQLPVKLPEDCVPDGSGNPLHKRADFIDCTCPKCGAPAKRETDTMDTFVD
                     SSWYYARYASGFSDKCMVDEQTNHWMPVDQYIGGIEHAILHLLYSRFWTKVMRDLGLV
                     NYDEPFANLLTQGMVLNHIFSRRTDKGGIEYFAPEEVELVHDEHGKVTGAKALKDGLP
                     VDFQGMGTMSKSKRNGVDPQSLIEQYGADTARLFMMFAAPPEQTLEWSDSGVEGSHRF
                     LRRVWSFGHVLSNEASIEMPAKLGGSLANARREIHATLKQANYDLARQQFNTVASAAM
                     KMLNTLEKLLKDAEPGWQAVAQEGFSILLRLLSPICPHITQALWKELGYENEGSGDIL
                     AANWPEPLEAALVQDEIELVVQVNGKLRGSITVARTLEKAAIEQHAVSQPFVQKFIEE
                     GATVRKIIVVPNKLVNIVVG"
BASE COUNT          888 a         1534 c         1638 g         1183 t
ORIGIN      
        1 tcggtgtgct gggaggaata ctggttgatg tgggcgatgg cctcgtcgac gccgctcacc
       61 accttgaccg agatgatggc atccaggtat tcggtgtaat agtcctcgtc cgtcgccgcc
      121 ttggcttcct tcaccagggc ccgcgtttca gcgcagccgc ggatctccac gcctttggcc
      181 aacagcatgg atgcgagttt cggtaatgcg tccgctgcga tgctgcgcgc gaccagcagg
      241 gattccgcgg tattgcaggt gccgagccgc tgtgtcttgg agttctccag gatcggcagc
      301 gctttagcca tgtcggcatc gtcatctaca tagatgtggc aattgccgtc cagatgcttg
      361 atgaccggga tgcgcgcctc gttcgagata cgctcgatca ggcccttgcc gccgcgtggc
      421 acgatgacgt cgacataatg tttcatggtg atgagctcgc ccacggcggc acgatcggtg
      481 gtctcgacga cctgcaccgc cgtgtgtggc aggccagccg cagtcaggcc ttcgtccacc
      541 agcgcggcca gcgcacgatt gcactggatg gcttcggaac cgccgcgcag gatggccgca
      601 ttgccggatt tgatgcacag cccggcagca tctaccgtca cgttcggacg tgcttcgtag
      661 atgatgccga tgacgcccag cggcacgcgc atgcggccga cctggatgcc ggaaggacgg
      721 tatttgaaat cgctcatctc gccgatcggg tcaggcaggc tggcgatctg ctccaggcct
      781 tcagccatgg tcgcgatgga tttttcgctc aaggtcagac gatcgagcat cgctggctcc
      841 agcccattct tgcgtgccgc ttcgaggtcg gcctggttgg cggcgagtag ttgagcctgg
      901 tcgcggcgga tcgccttggc gatcgtcagc aacgccagat tcttggtgtt ggcattggcc
      961 ttggccatcg cgcgcgatgc cagtcgggct tgctggccca ggccttccat gtattgcttg
     1021 atatccatag cgtgtctttt tatctggcgc tgttaccagc gcgattaacg tgagcgtttc
     1081 tgtatcctgg cgaggccgaa gcacagtcgg gagatctcga gccaggcgtc cccttgcagc
     1141 agccccttgg cggtacggtc gatctctgac agcttggctt gggccgccag cagctgtttc
     1201 aggctcagtc ggctgatggc gcgtcgtacc agcgcctgac ggtcgccgta gattctcgcc
     1261 tgttgcatga catttgccaa gttctcgcca ttggcctcgg cctgcttgat cctggccagc
     1321 ggtcgaatca cccaggaaag cgggttcatc acggccactg gctgggcgcc ttcgtctttg
     1381 agtccttgca ggatccgtat tgtacgttcg gcatcgcctg ctagcactgc atcgccgagc
     1441 tggaaggcat cgtagcgcga gacattgagc acggcttcgc gcacagcctg accgtcaagc
     1501 gcgccttgcg ggtagagcag accgagtttc tggatctcct gatgggccgc cagcagattg
     1561 ccttcgacct gatgcgccag aaacgccagt gtatcggcat cggtgtgctg accctgcaca
     1621 gccagtctat cgctgatcca gcgtggaagc tggtcggtcg gcacgtcgag caaggccagc
     1681 gagacgccgg cggattcgag agcggcatac caggcgctgt tcttgccgtc acggtcgagc
     1741 ttgggcagga taacgatgac tacggtatcg gccatgggct gctcggccaa tgcctgcaag
     1801 gccttgctgc cgtcggtgcc tggcttgccc gaagggatgt tcagttcaag gatgcggcgg
     1861 ctggagaaca gggagatcgc ctgaccgaag gccgcgacct gttgccagtt gaaattgcgc
     1921 tcgacgatca ggctagtgcg ttcgtcatat ccctgttggc gtgcagtagc gcggatggca
     1981 tccagacatt cgcgctgtgc cagcggttcg tcgcccatca agacatagag cggacgtaat
     2041 tccttggcga ggtgctgctg aagctgcgac tgttgcaggc gcacggctgg cttatttctt
     2101 ggtgaccttg taggccacca ggctgcgtac cagctgctgt gtggtttcct ggcgcatctc
     2161 ggcgttcagg cgctgttctt cgtaaccggt cgcaaggact tcacggtcgc tgtacgagaa
     2221 atcgcgacgc gtctcgatgg tcttgggctc tccccagagg tcttcatccg gctgcttgat
     2281 gcgataggtc accttgtaga tcaggtcgaa ttcgcggacc acaccggtac cgctcaacga
     2341 caggatgcgt ttctcgctga cctcgttcat gagatccatc atcagctcgg cgtcctcctt
     2401 ctcggtgacg acttcaacgc cgttcaccgc cagcgatttc ttcaggtcct tggagatgga
     2461 gatgttgccg ttgatgtaga gtttgcggaa cgacaggtct gcatagcctc gcatctggaa
     2521 gccgcaggcg gtcagcgcca gcaggctcaa aagcaggatg atgtgcagta gacgcttcac
     2581 ggacattggc ttatcccacg acgatattga cgagtttgtt cggtaccacg atgattttac
     2641 gcacagtcgc accttcttcg ataaattttt gcacgaaggg ctggctgacc gcgtgctgtt
     2701 cgatggccgc tttctctaac gtgcgggcga cagtgatact gccgcgcagc ttgccgttga
     2761 cctgcaccac gagctcgatc tcgtcctgca ccagggcagc ttccagcggt tcaggccagt
     2821 tggcggcgag gatgtcgccg ctgccctcat tttcgtagcc aagttccttc cagagcgcct
     2881 gcgtgatgtg cgggcagatc ggcgacagca ggcgcagcag gatgctgaag ccttcctggg
     2941 cgacagcctg ccagccgggt tccgcgtcct tgagcagttt ttccagtgtg ttcagcatct
     3001 tcatcgcggc cgaggcgacg gtattgaatt gctggcgtgc aaggtcgtaa ttggcttgct
     3061 tgagggtggc gtggatctcg cgacgggcgt tggcgaggct acctccgagc ttggccggca
     3121 tctcaatgga cgcttcattc gagagtacat ggccgaatga ccagacgcgt ctcaggaatc
     3181 ggtgcgaacc ctcgacgccg ctatccgacc actccagcgt ttgctccggc ggtgccgcga
     3241 acatcatgaa caggcgagcg gtatctgcgc cgtattgctc gatcagcgat tgggggtcga
     3301 cgccgttgcg tttggatttg gacatggtgc ccatgccctg gaagtccacc ggtagaccgt
     3361 ctttgagcgc cttggcgccg gtgaccttgc catgctcgtc atgcaccagc tcgacctctt
     3421 ccggcgcgaa gtattcgata ccgcccttat cggtgcggcg cgagaagata tggttgagca
     3481 ccatgccctg cgtcagcagg ttggcgaacg gttcatcgta attgaccagc ccgaggtcgc
     3541 gcatgacctt ggtccagaag cgtgaataca agagatgcag gatggcgtgc tcgatgccgc
     3601 cgatgtactg gtcgaccggc atccagtggt tggtctgctc gtcgaccatg catttgtcgc
     3661 taaaaccgct ggcatagcgc gcgtagtacc agctggaatc gacgaaggta tccatggtgt
     3721 cggtctcgcg cttggcgggc gcgccgcact ttgggcaggt acagtcgatg aaatcggcac
     3781 gcttgtgaag cggattgccg gagccatcgg gcacgcagtc ttccggcagc ttcaccggca
     3841 gctggtcgtc cggcaccggc acgtcgccac agcgctcgca atgaatgatc gggatcgggc
     3901 agccccagta gcgctggcgc gagatacccc agtcgcgtag acgccagttg atctgcttgt
     3961 cgccaaggtc cttggccttg aggtcggcgg caatggcatc gactgcggct tcgtaagcga
     4021 ggccgtcata cttgccggaa tgcgtgcagg tgccacgggt cttgtcgcca taccattcct
     4081 gccaaccagc cagtgagaac tcttcgcctt cgatggcgat gctctgcttg atcggcaggc
     4141 tgtacttctt ggcgaattcg aaatcgcgtt catcgtgtgc cggcacggcc atgacggcgc
     4201 cttcgccgta ggtcatgagc acatagttgc cgacccagac cggcacctgt tcgccggtca
     4261 acggatgggc gacggtgaga cccgtatcca tgccttcttt ttccatagtc gccatgtcgg
     4321 cttccatcac cgagccttgc ttgcacttct cgatgaaggc ggcgagggcg ggattcgagc
     4381 tcgcggctag cgtcgccagc ggatgttcgg cggccacggc gcagaaggtc acgcccatga
     4441 tggtgtcggc gcgtgtggtg aatacccaca gcttgccgtc gtcgatcaat tggccgctcg
     4501 tgtccttgat gtcatgggtg aaggcgaaac gcacgcccac gctcttgccg atccagttgg
     4561 cctgcatggt cttgacgcgc tcgggccagc ccggcaattt gtccaggtcg tccagcagtt
     4621 gttgtgcgta accggtgatg ttgaggtagt agccggggat ctcgcgtttc tccaccagcg
     4681 cgccggtgcg ccagccgcgg ccgtcgatca cctgctcgtt ggcgagtacg gtctggtcga
     4741 ccggatccca gttcaccacc tgcgtcttct tgtaggcgat gccttgttcc agcatgcgca
     4801 agaacagcca ctggttccac ttgtaatact cgggctgaca agtcgcgagt tcgcggctcc
     4861 agtcgatggc gaagcctaac gacttcagct gcttcttcat gtaggcgatg ttgtcgtaag
     4921 tccacttcgc cggcggtacc ttgttctgga tcgccgcgtt ctcggccggc aggccgaaag
     4981 catcccagcc catgggctgc agcacgttgt agcccttcat gcggtggtag cgcgtcagca
     5041 cgtcaccgat ggtgtagttg cgcacgtgcc ccatgtgcag cttgccgctg gggtagggga
     5101 acatggacag gcagtagtac ttcggcttgt ctgaatcggg cttggcgacg aaggattggt
     5161 tttgctccca agtctgctgg acggcttgtt ctagttcttt gaatggatac tgctgctgca
     5221 tggtggtcgc ggtcggataa atg
//
LOCUS       DATMTW010000280         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_628917, whole genome shotgun sequence.
ACCESSION   DATMTW010000280 DATMTW010000000
VERSION     DATMTW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628917"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VN084_05485"
     CDS             <1..428
                     /locus_tag="VN084_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28943.1"
                     /translation="TRSANMSIKRPRSFRVMLGLLATAGLVACASPDGDHGRHHADQS
                     AETAGNGLPGGPQSTGGMMGAGMTGSNGSCPMTDCGNATASTQRQPMMDKDAMCAMYR
                     GMRDAPTEQARQAMMERNMQAMSPEMRLQHMEMMRQQCQ"
     gene            complement(422..>1747)
                     /locus_tag="VN084_05490"
     CDS             complement(422..>1747)
                     /locus_tag="VN084_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HWT28944.1"
                     /translation="GFLAGNDRGASCLGPHSGLKMQEVRFKAAMDALTDPGRTTIILV
                     ARADKSAIAEAARTSEELHTLGLNNQRLVINGVFHASDPDDMVAVAIWTLEQRALDEM
                     PTCLQSLPQDQVPLRSFDTVGLAALRALFTANASPTIKSNAANDPIEPLPGLDALADE
                     LALAEHGLIMVMGKGGVGKTTLAAALALGLIRRGKTVHLSTTDPAAHLAGTLDAEVSG
                     LHVDRIDPKVETQRYIAKVMASKSSGLNAQEKALLLEDLRSPCTEEVAVFHAFSKIVN
                     EARSAFVVLDTAPTGHSLLLMDATGAYHRQTLREFEGLGSGRVTTPLMRLQDGTYTKI
                     ILVTLPEVTPVSQAVALQDDLRRAGVEPYAWVINKSILAAGTLDPLLQARMAGEREQM
                     RRVADGLAERLFVVPWRTTPPVGNLELSRLVSKPQDKEDADDGRPASVH"
BASE COUNT          355 a          537 c          522 g          333 t
ORIGIN      
        1 gaacaaggag cgcgaacatg tcaatcaaac ggccccgttc ttttcgagtg atgcttggat
       61 tgctcgcgac agcgggcctt gtcgcgtgtg ccagtcctga cggcgaccat ggtcggcatc
      121 acgcggatca atctgcagaa accgctggca atggcttacc gggtggcccc cagtcgactg
      181 gcggaatgat gggcgccggc atgacaggaa gcaatggcag ctgcccgatg acggattgcg
      241 gaaatgccac cgcctctacg caacgacagc cgatgatgga taaggatgcg atgtgcgcca
      301 tgtaccgcgg catgcgcgac gctccgacag agcaggctcg gcaagcgatg atggaacgga
      361 acatgcaagc gatgtctccc gagatgcgcc tacaacatat ggaaatgatg cggcagcaat
      421 gtcagtgaac cgacgctggc cgcccgtcgt cagcatcttc cttgtcctgc ggtttactga
      481 ccagcctgga taactcgagg ttgccaactg gtggcgtagt acgccagggc acgacgaaca
      541 aacgctctgc caatccatca gccacgcgtc tcatctgctc gcgctcgccg gccatccgcg
      601 cctgtagaag cgggtcaagc gtgccggcag caagtatgct cttgttgatc acccacgcat
      661 agggttcgac gccggcgcgg cgcaagtcgt cctgtaacgc cactgcttgc gaaacgggcg
      721 tcacttccgg caaggtcacc aggatgatct ttgtgtaggt tccatcttgt agccgcatca
      781 agggcgtggt cacgcgtcca ctgccaaggc cctcgaattc gcgcagcgtt tgccgatgat
      841 aggcaccggt agcatccatc aacagcaggg agtgcccggt tggcgccgtg tcgagcacga
      901 cgaaggcact ccgggcttcg ttgacgattt tcgagaaggc gtgaaaaacc gctacttctt
      961 cggtgcacgg cgagcgcaaa tcttccagca acaaggcttt ttcctgcgca ttcaaccccg
     1021 acgatttgct tgccatcacc ttggcgatgt agcgctgggt ttcaaccttc gggtcgatgc
     1081 gatcgacatg cagaccagac acctccgcat ccaacgtgcc ggcaaggtgc gctgccggat
     1141 ccgtggtgct gaggtggacg gttttcccgc gtcggatcag accgagcgcc aaggcggcgg
     1201 ccagggtcgt cttgcccacc ccgcccttac ccatcaccat gatcaaaccg tgttcggcaa
     1261 gtgccagctc gtcggccaac gcgtccaggc cgggcagcgg ctcgatggga tcattggcgg
     1321 cattgctctt gatggtcggg ctcgcatttg ccgtaaaaag cgcacgcaag gcagcaagcc
     1381 cgaccgtgtc gaacgatcgc agcggcacct gatcttgcgg taatgattgc aggcatgtcg
     1441 gcatctcatc caatgcccgc tgttccagag tccagatggc aaccgcgacc atatcgtcgg
     1501 gatcgctggc gtggaacacc ccattgatga ccagacgctg gttgttcagg ccgagcgtgt
     1561 gcaattcctc cgacgtgcgc gccgcctcgg caatcgcact cttgtcggcg cgggcgacca
     1621 gaatgattgt cgtccggccc ggatcggtca gggcatccat cgcagccttg aagcgtactt
     1681 cctgcatctt caagcccgaa tgcgggccaa gacaggaggc gccacgatcg ttaccggcca
     1741 gaaaacc
//
LOCUS       DATMTW010000281         1746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_325984, whole genome shotgun sequence.
ACCESSION   DATMTW010000281 DATMTW010000000
VERSION     DATMTW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325984"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="VN084_05495"
     CDS             complement(<1..1729)
                     /locus_tag="VN084_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HWT28945.1"
                     /translation="MLVGTCDNVLVAVSLLVAMLASYTALDMAGRVATVQGRAAWWWL
                     GGGAFAMGFGIWSMHFVGMLAFSLPIPLGYDIKITLLSLVIAIGVSAFALRQVCAAEL
                     SWQRLVRGALLMGIGIAAMHYTGMAAMLMMPPIQYDPWLFALSIVIAVVASGAALWIF
                     FQLRKNRAYLVLYRAGAAVVMGVAIAGMHYTGMAAANFPSNSFCGAASGSFDTHWLAL
                     LVVVMTLAVLAIALITSVLDARMESRTSMLAKSLAKANRELSELALQDTLTKLPNRIL
                     LEDRLEHALSLAKRESTVFALMFMDLDGFKAVNDAYGHDVGDRLLIEVAGRVRDLLRV
                     QDTVARLGGDEFVMLLEQTDPDGAASVADKLIYALSQPYRIGKREMRVSASVGIAMYP
                     GDGENAKTLMTNADAAMYHAKNSGRNGYFFFETSMNENAHDQLQLMQDLQVALERNEF
                     VLHYQTKYVAPAGPITGAEALLRWQHPTRGLVPPDRFIPYAEKTGLIVPIGEWVLNEA
                     CRQMREWYLQGCRNWKVAVNLSPVQFSHPNLINVVRNALESHRLPPHCLTLEVTETTA
                     MRNADVSLAI"
BASE COUNT          343 a          562 c          547 g          294 t
ORIGIN      
        1 gaatcgcgag gctcacgtcg gcattgcgca tggccgtggt ttcggtcact tccagcgtca
       61 ggcagtgcgg cggcaagcgg tggctctcca atgcattgcg cacgacattg atcaggttcg
      121 ggtgcgagaa ctgcaccggc gacagattca ccgccacctt ccagttgcgg cagccctgca
      181 ggtaccactc gcgcatctgg cggcaggctt cgttgagcac ccattcgccg atgggcacga
      241 tcaggccggt cttttccgcg tagggaatga agcggtccgg cggaaccagg ccgcgcgtcg
      301 ggtgctgcca gcgcagcagg gcttcggcgc cggtgatcgg accggccggc gcgacgtact
      361 tggtctggta gtgcagtacg aattcgttgc gctccagcgc cacctgcagg tcctgcatca
      421 gctgcaactg gtcgtgcgca ttctcgttca tcgacgtttc gaagaagaag tagccgttgc
      481 gaccggagtt cttggcgtgg tacatggcgg catcggcatt cgtcatcagc gtcttggcgt
      541 tttcgccgtc gcccggatac atggcgatgc cgacgctcgc agacacgcgc atttcgcgtt
      601 tgccaatgcg atacggctgg ctgagtgcat agatcagctt gtcggcgacg ctggcggcgc
      661 cgtcgggatc ggtctgctcc agcagcatca cgaattcgtc gccgccgagc cgcgccaccg
      721 tatcctgcac gcgcagcaga tcgcgcacgc ggccggcgac ctcgatcagc aggcggtcgc
      781 ccacatcgtg cccgtaggca tcgttgaccg ccttgaagcc atcgaggtcc atgaacatca
      841 aggcaaacac ggtggattcg cgcttggcca gactcagcgc atgctccagc ctgtcttcga
      901 gcaggatgcg gttgggcagc ttggtcagcg tgtcttgcaa ggccagctcg ctgagctcgc
      961 ggttggcctt ggccagcgat ttcgccagca tggaagtgcg cgactccata cgcgcgtcca
     1021 gcaccgaagt aatcagggca attgccagaa ccgccagggt catgacgacg acgagcagcg
     1081 ccagccagtg cgtgtcgaag gagccgctcg ccgcgccgca gaaactgttc gagggaaagt
     1141 ttgccgcggc catgccggtg taatgcatgc cggcgatagc cacgcccatg accacggcgg
     1201 cgcccgcgcg atacagcacc agataggcgc ggttcttgcg gagctggaag aagatccaca
     1261 gcgcagcgcc cgaggccacg acggcgatga cgatggagag cgcgaacagc cacgggtcgt
     1321 actggatcgg cggcatcatc agcatggccg ccatgcccgt gtagtgcatc gccgcaatgc
     1381 cgatgcccat gagcagcgca cctctgacca ggcgttgcca cgacagctcg gcggcgcaca
     1441 cctgacgcag cgcgaaagcc gatacgccaa tcgcgatgac cagcgacagc aatgtaattt
     1501 ttatatcgta gccaaggggt atcggcaggc tgaaggcaag catgccgacg aagtgcatgg
     1561 accagatgcc gaagcccatc gcgaacgcac cgccgcccaa ccaccaccaa gccgcacgcc
     1621 cctgcaccgt tgccacacga ccggccatgt cgagcgccgt gtaggacgcc agcatggcca
     1681 ccaggagcga cacggcgaca agtacattgt cacaagttcc gaccaacatc cagcttcctc
     1741 caagca
//
LOCUS       DATMTW010000282         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_662818, whole genome shotgun sequence.
ACCESSION   DATMTW010000282 DATMTW010000000
VERSION     DATMTW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662818"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VN084_05500"
     CDS             complement(<1..736)
                     /locus_tag="VN084_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWT28946.1"
                     /translation="MENQLQNVDHLKNTLIAIAFQFGPKVIVASLIIVVGVYVGRWIG
                     RLLEGGLKKFHLEAPVEQLLVRIVRILVLALFVIMALQNLGIELLPLIAGLGVAGAGV
                     ALAMQGVLGNIVAGLTIIFTKPFKLGEYISIVGEEGRVESISLFTTELSHTDLSRVVI
                     PNRKIVGEILHNYGRIRQLDLEVSVAYDSDLDHALAAVREILDHNPRVLKMPPAVVNV
                     RVLGASSISIAVKPWIEVPDYVPAVGE"
     gene            complement(758..1510)
                     /locus_tag="VN084_05505"
     CDS             complement(758..1510)
                     /locus_tag="VN084_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWT28947.1"
                     /translation="MNSAPIKVLIVDDEAPARNRLRDLLADIGQVQVVGEARNGEEAV
                     QLATAHQPQLVLLDIRMPGVDGIEAAGHLQKMQPPPAIIFTTAYDTYAMQAFDMNAID
                     YLLKPVRQERLESAIGKARALLPAQWQALKPLQTQRTHFSIVERGRVILVPVADVIYL
                     RAELKYLTVRTREREYLMEDSLTAIEQEFGNRFLRLHRNCLVAESSILGYEKRHDAGG
                     ETRWVALLRELPDTIAVSRRQHHVLRNLDHRQ"
     gene            complement(1507..2496)
                     /locus_tag="VN084_05510"
     CDS             complement(1507..2496)
                     /locus_tag="VN084_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWT28948.1"
                     /translation="MSKLPDFRNLGTCLRILLSANLLALLAALLLAMDPAAILTVLMQ
                     ISVVVQPILLLSLSLLYGLSPQLRRLNYQAGIVSILLLCQFVGLSVQLGASFILPDAG
                     VPDPARVSLLTLVASGMVLFYLHLRQRALTPAISEARLQALQARIRPHFLFNSINAVL
                     SIVRSEPARAETALMDMADLFRVLMADNRDLVPLADELALCRQYLALEKLRLEERLQV
                     SWQIDNMPPDALVPPLILQPLLENAVYHGIEPLPAGGKIVINIFADRDGAHLILSNPY
                     VPQSAHPNGNKMALANIRERLALHFDAEASLKSELAGDQYQVHIVLPYRRSAA"
BASE COUNT          481 a          723 c          820 g          472 t
ORIGIN      
        1 tttcccccac ggccggcaca taatccggca cctcgatcca aggtttgacc gcgatgctga
       61 tggaggaggc gccgagcact ctgacattga ccacggctgg cggcatcttg aggacgcgcg
      121 ggttgtggtc gaggatctcg cggacggcgg ccagggcgtg gtcgagatcg ctgtcataag
      181 cgacgctgac ttcgagatcg agctggcgaa tcctgccgta gttatgcagg atctcgccga
      241 cgatcttgcg gttggggatg accacgcgag agaggtcagt gtggctgagc tcggtggtga
      301 agaggctgat gctctcgacg cgaccttcct cgccgacgat ggagatgtat tcgccgagct
      361 tgaagggctt ggtgaagatg atggtgaggc cggcgacgat attgccgagc acaccttgca
      421 tggccagcgc gaccccggcg ccggcgacgc cgagcccggc gatcagcggc aagagttcga
      481 tcccgaggtt ctgcagcgcc atgatgacga atagcgccag caccaggatg cgcacgatac
      541 gcaccagcag ctgctccacc ggcgcctcga ggtggaattt cttgaggccg ccctcaagca
      601 gtcggccgat ccagcggccg acatagacgc cgacgacgat gatgaggctg gccacgatga
      661 ccttgggacc gaactggaag gcgatggcga tcagggtgtt cttgaggtga tccacgttct
      721 gcagctgatt ttccatacga ggctctcttt gtcgtgatta ttggcggtga tccaggttgc
      781 gcaacacatg atgttggcgt cggctgacgg cgatggtatc cggcaactcg cgcagcagtg
      841 ccacccagcg cgtctcgcca ccggcgtcat ggcgtttctc gtaaccgagg atgctgctct
      901 cggcgaccag acagttacga tgcagtcgca ggaaacggtt gccgaactcc tgttcgatgg
      961 cggtcagcga gtcttccatc aggtactcgc gttcccgcgt gcgcaccgtc aggtacttga
     1021 gttccgcgcg caggtagatg acgtccgcca ccggcaccag gatcacgcga ccacgctcga
     1081 cgatgctgaa atgcgtgcgc tgggtctgca atggtttcag ggcttgccac tgggccggta
     1141 gcaaggcgcg ggccttgccg atcgccgact ccaggcgctc ctgccgaacc ggtttcagca
     1201 ggtagtcgat ggcgttcatg tcgaaggctt gcatggcgta ggtgtcgtag gccgtagtga
     1261 agatgatcgc agggggcggt tgcatcttct gcagatggcc ggcggcttcg atgccgtcca
     1321 cgccgggcat gcggatatcg agcaacacca gttgcggctg gtgggccgtc gctagctgca
     1381 ccgcttcctc gccgttccgc gcttcaccga ccacctgtac ctgtccgatg tctgccagca
     1441 ggtcgcgcag acgattgcgc gcaggcgctt cgtcatccac gatcaggact ttgatcggcg
     1501 cggagttcaa gcggcgctcc tgcgataggg cagcacgata tgcacctggt actgatcgcc
     1561 ggccaactcg cttttgagcg aggcttccgc atcgaaatgc agggcaagtc gctcccggat
     1621 attggccagg gccatcttgt taccgttcgg atgggcactt tgcggcacat aagggttgct
     1681 caggatgagg tgggcgccat cacgatcggc aaagatattg atgacgatct taccgcccgc
     1741 gggcaagggt tcgatgccgt gatagacggc gttttccagc agtggctgca ggatcagcgg
     1801 cggtaccagg gcgtccggcg gcatgttgtc gatctgccag ctgacctgca gtcgctcctc
     1861 cagccgcagc ttttccagcg caaggtattg ccggcacagc gccaattcgt ccgcgagcgg
     1921 caccaggtcg cggttatccg ccatcagcac gcggaacagg tctgccatgt ccatgagtgc
     1981 ggtctcggcg cgcgccggtt cgctgcgcac gatggaaagc acggcgttga tgctgttgaa
     2041 caggaaatgc gggcgaatac gcgcttgcaa ggcttgcagg cgggcttcgg agatggccgg
     2101 ggtaagtgct cgctgacgta gatgcagata gaacagcacc atgccgctag cgaccagcgt
     2161 caacaggctc acgcgtgccg ggtccggtac gccggcatcc ggcaggatga agcttgcgcc
     2221 gagttgcacg ctaaggccga cgaactggca cagcagcaat atgctgacga tgccagcctg
     2281 gtagttgagt cgacgcagct ggggcgagag cccatagagc aggctcagcg acaacaataa
     2341 aatcggctgc acgacgacgg aaatctgcat gagaacggtt aaaatcgcag ccggatccat
     2401 cgccagtagt agcgcagcca gcaaagccag caggttggcg ctcagcagga tgcgcaggca
     2461 agtgcccaga ttgcggaaat ccggcagttt gctcag
//
LOCUS       DATMTW010000283         5095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_461096, whole genome shotgun sequence.
ACCESSION   DATMTW010000283 DATMTW010000000
VERSION     DATMTW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461096"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VN084_05515"
     CDS             complement(<1..461)
                     /locus_tag="VN084_05515"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWT28949.1"
                     /translation="MVGHVPVGGDAPVVVQSMTNTDTADAAATVQQVFELWQAGSEVV
                     RITVNSPEAAAQVATIRRQLDAMGCNVPLVGDFHYNGHKLLAQYPECAEALAKYRINP
                     GNVGKGSKRDEQFSAMIETAIKYDKPVRIGVNWGSLDQEKMARMMDENAKRA"
     gene            complement(507..1397)
                     /locus_tag="VN084_05520"
     CDS             complement(507..1397)
                     /locus_tag="VN084_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWT28950.1"
                     /translation="MSDTEKTDTAEVVGQQAAIGASLKAAREARGLSVEDVASHLRLS
                     PRQIHALENDEFSALPETTITRGFIRNYARLLEIAAEPLLDAYKAYSPAGGPKAITIQ
                     SANILISGGDRRPWVAYISASIVIAVLLGAWLLYVEPARVGAPKAIAVAPAPTVSDTA
                     SNEVTEPLPVAALPAAERAQAAEPVPDAAAANTETAPAPAPEANATSAPMDPQDASRA
                     LAKVELRFTESSWVSVVDRNDREILNKTKPAGTAEQIEGQPPLKLIIGNAAGSVVVFN
                     GKTVDLGPYTKLNVARITLE"
     gene            complement(1397..2143)
                     /gene="pilW"
                     /locus_tag="VN084_05525"
     CDS             complement(1397..2143)
                     /gene="pilW"
                     /locus_tag="VN084_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HWT28951.1"
                     /translation="MKWLSLLLALMLTACAGQPERPAYGGESNLSERARIHTELGAGY
                     YAQNQLGIALEEFKEAVQIDPSYSLGHNGLGLVYAALREDAKADASFKKAIELAPNNS
                     ESRNNYGTFLCSRGRYDESITQFLEAVKNPLYVTPGVAYMNAGVCSLRKKDVVNAEVY
                     LQRALQVQPVLYQASYQLAQIEYDRGQPMRARQYLQTPLANDPTAEMLWLGIKVERLL
                     GGRDAEASYALQLRKKYPNSEQTKALLSGQ"
     gene            complement(2140..3258)
                     /gene="rlmN"
                     /locus_tag="VN084_05530"
     CDS             complement(2140..3258)
                     /gene="rlmN"
                     /locus_tag="VN084_05530"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWT28952.1"
                     /translation="MVNLLDLNQSQLAEYFQKLGEKPFRAKQLMRWMHHFGVADFEQM
                     TDIAKSLREKLSLEASITPPEVQFEQVSDDGTRKWLIGAGGGNGVETVFIPEADRGTL
                     CVSSQVGCALECTFCSTGRQGFNRNLSVAEIIGQLWVANRSLRQADGYDMLPPGERII
                     SNVVMMGMGEPLANYDNVVTAMQIMLDDSAYGLSRRRVTLSTSGMVPAMDRLKEDCPV
                     ALAVSLHAPNDALRDEIVPINKKYPLKALMAACERYLVKAPRDFVTFEYVMLDGINDT
                     VEHARQLLDVVRDVPCKFNLIPFNPFPNSGYGTSKPDSIRRFRDVLMQAGYVVTTRKT
                     RGDDIDAACGQLAGQVQDKTKRSERHRTFKNIAIEVTQ"
     gene            complement(3273..3680)
                     /gene="ndk"
                     /locus_tag="VN084_05535"
     CDS             complement(3273..3680)
                     /gene="ndk"
                     /locus_tag="VN084_05535"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWT28953.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGKIYTRFEDAGLKIVNAKMAQLSQ
                     ADAEGFYAVHKERPFFKDLVKFMISGPVMIQVLEGEGAVAKNRELMGATNPKEAAAGT
                     IRADFAESIDANAVHGSDSAENAAIEIAYFFGK"
     gene            3924..>5095
                     /locus_tag="VN084_05540"
     CDS             3924..>5095
                     /locus_tag="VN084_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWT28954.1"
                     /translation="MATYRSTFSIRRLWLILASSMVLMFAILLYFGHEIYQEAPPIPD
                     KVESDGGEVLYTNADIARGQNVWQSIGGMQQGSIWGHGGYLAPDWSADWLRREAEALR
                     DLLAGTAGQEAPEVADALLRKQLRTNTYDPASGILRVSEARAQAIRQVAEHYRGLFQA
                     DAAYLDLRRAYAMPLHATLDENDAHALTAFFFWTAWSAVTNRPGDDISYTSNWPHDPL
                     VGNTPSAGVLLWSLISILVLLGGIGAIVWYYARQYDDWRNDQAPEEGYAQEDRMASLT
                     ITPSMRATAKFFWVVTALFLMQIGLGVITAHYAVEGQGLYGLPFAEYLPYVVTRTWHT
                     QLAVLWIATAWLATGLYVAPLLSGHEPRFQRLGVNFLFWSLLLIVAGSFAGEWLAV"
BASE COUNT          977 a         1518 c         1541 g         1059 t
ORIGIN      
        1 gcacgcttgg cattctcgtc catcatgcgc gccatctttt cctggtcgag gctaccccag
       61 ttgacgccga tgcgcaccgg cttgtcgtac ttgatcgcgg tctcgatcat ggccgagaac
      121 tgttcgtcgc gcttcgatcc cttgccgaca ttgcccggat tgatacggta tttggccagt
      181 gcctcggcac attcaggata ctgcgccagc agcttgtggc cgttgtaatg gaaatcgccg
      241 accaaaggca cattgcaacc catggcgtcc agctggcgtc ggatcgtcgc gacctgggct
      301 gcggcttcgg gagaattgac ggtaatacgg accacttcgg aacccgcctg ccaaagctcg
      361 aatacctgct gcacggtggc tgcggcatcg gcggtgtcgg tattggtcat cgactgcacg
      421 accaccgggg catcgccccc gaccggcaca tggccgacca tggcctggag gctctgacga
      481 cgttggatgg ctaagctgtt caagatctat tccagagtga ttcgcgcaac gttgagcttg
      541 gtgtaggggc caagatcgac cgtcttgcca ttgaaaacga cgactgaacc tgcagcattg
      601 ccgatgatga gcttgagcgg cggctggccc tcgatctgtt cggcagtgcc cgcgggcttg
      661 gtcttgttga ggatctcacg atcgttacga tcgaccacac tcacccagct ggattcggtg
      721 aaacgcaatt caaccttggc cagcgcacgg gatgcgtcct gcgggtccat aggcgcagag
      781 gtggcgttcg cttccggcgc tggagccggt gctgtttcag tattggccgc agctgcgtct
      841 ggcacaggtt ctgcggcttg cgcacgctca gcagccggca aggctgcgac aggcaaaggt
      901 tcggtaacct cgttggatgc cgtgtcggac acggtcggcg ccggggctac tgcgatggcc
      961 ttcggcgcac cgacgcgcgc cggctcgaca tacagcagcc atgcgcctag caagaccgcg
     1021 atgacgatac tggccgagat gtaagcgacc cagggtcggc ggtcgccgcc agagatcagg
     1081 atattggcgg actggatggt gatagctttt gggccgccgg ctggcgagta agccttgtag
     1141 gcgtcgagca aaggctctgc ggcgatctca agcaatcgtg cgtaattgcg gatgaaaccg
     1201 cgggtgatgg tggtttcagg cagggccgag aattcatcgt tttccagcgc gtggatctgg
     1261 cgcgggctga gacgcaggtg gctggccaca tcctcgacag aaagccccct ggcctcccgg
     1321 gctgccttta gactggcgcc gatggcggct tgctggccca ccacttccgc cgtgtcggtc
     1381 ttttccgtat cgctcatcac tgccccgaca ataacgcttt ggtttgttct gaattgggat
     1441 atttcttgcg caactgcaac gcgtagctcg cctctgcgtc gcggccgccc agcaatcgct
     1501 cgaccttgat gccgagccac agcatctctg cagtcggatc gttcgccagt ggcgtctgca
     1561 gatactgccg ggcgcgcatg ggctggccac ggtcatactc gatctgcgct agctggtaag
     1621 acgcctggta tagcacgggc tgcacctgca gggcgcgctg cagatagact tcggcattga
     1681 cgacatcctt cttgcgcaag gagcagacgc cggcgttcat gtaggccacg ccgggcgtca
     1741 catacagcgg gttcttgacg gcttcgagga actgcgtgat ggattcgtcg taacgcccac
     1801 gtgaacaaag gaatgtgccg taattgttgc gcgattcaga attgttaggc gccagttcga
     1861 tcgctttctt gaagctggcg tcggccttcg catcttcacg cagcgccgca tagaccagcc
     1921 ccaagccgtt atggcccagc gagtaagagg gatcgatttg caccgcctcc ttgaactctt
     1981 caagagcgat gcccagctgg ttctgggcat agtaccctgc gcccagctcg gtatggatgc
     2041 gcgcacgttc actgagattg gactcgccgc cataggcagg acgctcaggt tgcccagcgc
     2101 aagcagtcag catgagggca agcagcaggg acagccattt cattgagtta cctcgatagc
     2161 aatattcttg aaagtccggt gacgttcgct gcgtttggtc ttgtcctgca cctggccggc
     2221 caattggcca cacgcggcat cgatatcgtc accgcgtgtc ttgcgcgtgg tcacgacgta
     2281 accggcttgc atcaacacat cgcggaaccg gcggatactg tccggcttgg aagtcccgta
     2341 accactgttg gggaaagggt tgaacgggat caggttgaac ttgcagggca catcgcgcac
     2401 gacatccagc aattggcggg cgtgctcgac cgtgtcgttg atgccatcca gcatcacgta
     2461 ttcgaatgtg acgaaatccc gcggcgcctt taccaggtaa cgctcacagg ctgccatgag
     2521 ggctttcagc ggatatttct tgttgatcgg cacgatctcg tcgcgcaacg cgtcattcgg
     2581 cgcgtgcagc gaaacagcca aggcgaccgg gcaatcttcc ttgagccgat ccatggccgg
     2641 caccataccg gaggtagaca gggtcacgcg gcggcggctc aggccataag ccgaatcgtc
     2701 gagcatgatc tgcatggcgg tgacgacatt gtcgtaattg gccagcggct cgcccatacc
     2761 catcatgacc acgttactga tgatgcgctc gcctggcggc agcatatcgt agccatcggc
     2821 ttgccgcagc gatcggttcg cgacccaaag ctggccgatg atctcggcga cactgagatt
     2881 gcgattgaag ccttggcggc cggtggagca gaacgtgcac tctagggcgc aaccgacctg
     2941 ggaagagacg cacagggtgc cacgatcggc ttcggggatg aacacggtct cgacaccatt
     3001 gccaccgcct gcaccgatca gccatttacg cgtgccgtca tccgacacct gctcaaactg
     3061 gacctcaggc ggcgtgatac tggcttccag cgacagcttc tcacgcaggg atttagcgat
     3121 atcggtcatc tgctcgaaat cggccacgcc gaaatgatgc atccagcgca tcaactgctt
     3181 ggcgcgaaac ggcttctcgc cgagcttctg gaagtactcg gcgagttgcg actggttcag
     3241 atcgagcagg ttgaccaata ctggatgcct ggttacttac cgaagaagta agcgatctcg
     3301 atcgctgcgt tttcggcgga atcgctaccg tgtacggcgt tggcgtcgat gctctcggcg
     3361 aaatcggcgc gaatggtgcc cgcagcggct tccttcgggt tggtggcacc catcagctcg
     3421 cgattcttgg cgacagcgcc ctcgccttcc agcacctgga tcatgactgg accggaaatc
     3481 atgaacttga ccagatcctt gaagaaagga cgctccttat gcacggcgta gaagccctct
     3541 gcgtcagctt ggctcaattg ggccatcttg gcgttgacga tcttcaagcc cgcgtcttcg
     3601 aaacgggtgt agatcttgcc gatgacgttc ttggcgactg cgtcgggttt gatgatggaa
     3661 agggtgcgtt caacagccat atttgctccg aaaattcaag tggatgaaag ggtgcaagaa
     3721 taccattttg acgcgcttaa ataaaccttg aatccgcgca tcaacggctt ttttgcggca
     3781 gcgcacgcat gtcatcgaca tccgcacgcc gacacgatac ttggcgagat cactgccttg
     3841 tgctttaaga tgtcggcaag cgaagcagct tcaccgcaac cctaccgccg ggcaaaccgg
     3901 ccatcacttc cgggaggcat gacatggcga catatcgcag caccttctcc atcaggcgtc
     3961 tctggctgat ccttgccagc agcatggtcc tcatgttcgc gatcctgctc tacttcgggc
     4021 atgagatcta ccaagaggca ccacccattc cggacaaggt cgagagcgat ggcggcgagg
     4081 tgctctacac gaacgccgac atcgcccgtg gccagaacgt ctggcaatcg ataggcggca
     4141 tgcagcaggg ttcgatatgg gggcatggcg gctatctcgc ccccgactgg agcgccgact
     4201 ggttaaggcg tgaagccgag gcgctacggg acctgctagc aggcactgcc ggccaagaag
     4261 cgccggaagt ggccgatgcc ttgctgcgca agcagctacg gacgaacact tatgacccgg
     4321 ccagcggcat actgcgcgtg agtgaagcgc gggcccaggc cattcgccag gtcgccgagc
     4381 attatcgcgg cttgttccag gcagatgcgg cttatctgga cctgcgacgg gcctatgcga
     4441 tgccgctgca tgccacgctg gacgagaatg acgcgcatgc gctgacggcc ttctttttct
     4501 ggactgcctg gagtgcagtg accaaccgtc ccggcgacga catcagctac accagcaact
     4561 ggccgcacga cccgctggta ggcaacacgc caagtgccgg tgtgcttttg tggagcctga
     4621 tctccatcct ggtgctgctg ggcgggatcg gcgccatcgt ctggtactac gctcgccagt
     4681 acgacgattg gcgcaacgac caggcaccgg aggaaggtta cgcccaggaa gaccgcatgg
     4741 cttcgctcac catcacaccc tccatgcggg cgaccgcgaa attcttctgg gtggtgactg
     4801 cgctgttcct catgcagatc gggctgggcg tcatcaccgc acactatgct gtcgagggcc
     4861 aaggcttgta tggcctgcct tttgccgagt acctgcccta tgtggtgacc cgtacctggc
     4921 acacacagct cgcggtgcta tggatcgcca ccgcctggct cgccaccggg ctctacgtgg
     4981 ctcccttgct gtcgggccat gaaccgagat tccagcgcct gggggtcaac ttcctgttct
     5041 ggagcctgct attgatcgtg gcaggctcat ttgcaggcga gtggctcgcg gtgca
//
LOCUS       DATMTW010000284         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_595886, whole genome shotgun sequence.
ACCESSION   DATMTW010000284 DATMTW010000000
VERSION     DATMTW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595886"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VN084_05545"
     CDS             <1..196
                     /locus_tag="VN084_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWT28955.1"
                     /translation="GLTVWRALIERGVYVNLVLPPATPGAVTLVRCSVSAAHTPEQID
                     RIIEAFASLRGAMGQVTAGA"
     gene            255..1982
                     /locus_tag="VN084_05550"
     CDS             255..1982
                     /locus_tag="VN084_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWT28956.1"
                     /translation="MRIIFAFGRSYPARTVLMLLSLLLAGVVESVSLSTMLPLISAML
                     EDQPHSKLAVYVFGAMNSIGLQPTIANMLVVIVVGMTLSNLLILLGNRQVGYTVAHVA
                     TDLRVSLIKALLGSRWEYYLRQPSGALANAVATEAFRASTAYEHAANMLALGIQAAAY
                     ALIALFVSWQAAILSLVIGFVLLAAVRGLVKTSRRVGKSQTRLMRELLVHLTDTLGSV
                     KPLKAMARDDIADSLLRRHAGELNTALEREVMSRETLRALQEPMLAILAAAGLYAGLV
                     IGGMPLSSVMVLIFVVVRMLGLLHKAQQRYHRMAAQESAYWAINESIDNAIADAEPVG
                     GTCTPSLQRHIAFENISFGYGGRQVLDNVSMNFPVGSFTTMIGPSGSGKTTVLDLICA
                     LLAPQSGRIRVDDCDLHALDRKAWRRMIGYVTQETLLLHDSILANVTLGDRALNAADA
                     ERALRQAGAWQFVQECPGGLQSIVGERGAKLSGGQRQRIVIARALAHRPRLLILDEAT
                     SALDPESEAEICQVLKNLTPGITVIAVSHRPAVIRIADQILEVRDGKVLATHHAVDPD
                     GTRADEAKA"
     gene            complement(2158..>2910)
                     /locus_tag="VN084_05555"
     CDS             complement(2158..>2910)
                     /locus_tag="VN084_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWT28957.1"
                     /translation="LIGKTVLVTGASSGIGAHLARVASDAGARVVLGARRVERLQQLA
                     DEIRQTGGEAMAVALDVTDRDSVEAAFDAAQAAFGVVDVVLNNAGIGNGQRVLEISEA
                     DWRAMLSTNLDGVWRVAQCAAQRLAKAQRPGSIVNIASMLGLRVGTGYSHYCAAKAGV
                     VQLSKSLALELARYQIRVNAIAPGYFKTEMNDEYFDSEKGQAYIRDTVPMRRLGSLQE
                     LEGPFLLLASEAGAFMTGAVLAVDGGHLVGSL"
BASE COUNT          521 a          913 c          900 g          576 t
ORIGIN      
        1 gggcctgacc gtttggcggg cgctgataga gaggggcgtc tatgtcaatc tggtccttcc
       61 tcccgcgacc cccggcgccg tcaccctggt gcgttgcagc gtcagtgcgg cccatacacc
      121 tgaacaaatt gaccggatca tcgaggcctt cgcttcattg cgtggcgcga tgggccaggt
      181 cacggcgggc gcttgatgag tgtcgtgagc tgtcggtgag ctaatcttta cctacgtcat
      241 agtgaaccac agtcatgcgg attatttttg cctttggccg ctcctacccg gcccgcaccg
      301 tgctgatgct cctgagcctg ctgctggcag gggtggttga aagcgtcagt ttgagcacga
      361 tgctgccctt gatatcggcc atgcttgaag accagcctca ttcgaagctg gcggtctacg
      421 tcttcggggc aatgaactcg atcggcctgc agccgacgat cgcgaacatg ctggtggtca
      481 ttgtcgtggg catgacgctg agcaacctgc tgatactgtt gggcaaccgt caagtgggct
      541 ataccgtggc acacgtggca accgacctgc gtgtgagcct catcaaggca ttgctgggta
      601 gccgctggga atattacctg cgccaaccct ccggagcact ggccaatgcc gtggcaaccg
      661 aagccttccg ggcttcaaca gcctatgagc atgcggccaa tatgctggcg ctgggtatcc
      721 aggcggcggc ctatgcacta atcgcgcttt ttgtctcgtg gcaggccgcg atcctgtcac
      781 tggtgatcgg cttcgtcctg ctggctgccg tgcgaggtct ggtcaagaca tcgcggcgtg
      841 tcgggaaaag ccagacccgc ctgatgcgcg agttgctggt ccacctgacg gacacccttg
      901 gctcggtgaa accgctcaaa gccatggcac gcgatgacat cgccgattcc ttgctgcgtc
      961 gtcacgccgg cgaactcaat accgcattgg aacgcgaagt gatgagccgc gaaacgctgc
     1021 gggccttgca ggagcccatg ctggcgatcc ttgccgcggc gggcctctac gcggggctgg
     1081 tcatcggcgg catgccgctg tcttcggtga tggtgttgat cttcgtggta gtgcgcatgc
     1141 tggggttgct gcacaaggca cagcagcgct atcaccggat ggccgcgcaa gagagcgcct
     1201 attgggccat taacgagtcg atcgacaacg ctattgccga tgccgagcct gtcggaggga
     1261 cttgcacgcc ttccttgcag cggcatatcg cttttgaaaa tatcagtttt ggctacggcg
     1321 ggcggcaggt tcttgacaac gtgtcgatga atttcccggt cggttcattc accaccatga
     1381 tcggtccttc cggctccggc aagaccaccg tgctcgacct gatctgtgcg ctgctcgcgc
     1441 cacagtccgg acgcatcagg gtcgacgact gtgacttgca tgccctggac aggaaggcct
     1501 ggcgccgaat gatcggctac gtcacccagg aaaccctact gctgcacgat agcatcctgg
     1561 cgaacgtcac cttgggagac agggccttga acgccgccga cgcagagcgc gcgctgcgtc
     1621 aggccggtgc atggcagttt gtgcaggaat gccctggcgg actgcaatcg atcgtcggcg
     1681 agcgcggggc caaactctca ggagggcaaa ggcagcgtat cgtcatcgcg cgtgccctgg
     1741 cccatcgccc caggctgctg atcctggacg aagccaccag cgccctcgat cccgagtcgg
     1801 aggccgaaat atgccaggtg ctgaagaatc tcacgcctgg catcacggtc atcgcagtgt
     1861 cgcacaggcc tgccgtgatc cgcattgcgg atcagattct cgaggtccga gacggcaagg
     1921 tactggccac gcaccatgcc gtcgatcctg atggcactcg ggctgacgag gcgaaagcct
     1981 gagaaaagcg caggggtagg gggcgtccgg tctgtaggag cgagcatgct cgcgaaaaac
     2041 ctgagagcac cgcggcgtgt caggctcctc gcgtcatcgt tgacctccat cgtcggaacg
     2101 ccgcccggag catgctcgct cctacagaga tatccgttca ttcgcgggag ctagcgttta
     2161 caggctgccg accagatggc cgccgtccac tgccaggacc gcgcctgtca tgaaggcgcc
     2221 ggcctcgctc gcgagcagga gaaacgggcc ttccagttcc tgcaggcttc ccagtcgtcg
     2281 cattggcacg gtgtcgcgta tatacgcctg gcccttttcg ctgtcgaaat actcatcgtt
     2341 catttcggtc ttgaagtaac ccggcgcaat ggcattcacg cggatctggt agcgcgccag
     2401 ttccagcgcc agggatttgc tcagttgcac cacgccagcc ttcgccgcgc agtagtggct
     2461 gtagccagtg cctacgcgca gcccgagcat cgaggcgatg ttgacgatgc tgccaggtcg
     2521 ctgcgccttt gcgaggcgtt gcgccgcaca ctgggcaacg cgccagacgc cgtcgaggtt
     2581 ggtcgacagc atcgcccgcc aatcggcttc gctgatctcc agcacgcgct ggccgttgcc
     2641 gatgccggca ttgttgagca ctacatccac cacaccgaac gcagcttggg cggcgtcaaa
     2701 cgctgcttcg acgctgtcgc gatcggtaac gtccaaggcc acggccatag cctcaccgcc
     2761 agtctggcga atttcgtctg ccagttgctg caagcgctcc acgcgccggg cgcccagtac
     2821 tacgcgcgca cccgcatcgc tggccacgcg cgccagatgc gcgccaatgc cgctggaggc
     2881 accggtgacc agtaccgtct tgccaatcag
//
LOCUS       DATMTW010000285         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_157951, whole genome shotgun sequence.
ACCESSION   DATMTW010000285 DATMTW010000000
VERSION     DATMTW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157951"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VN084_05560"
     CDS             <1..602
                     /locus_tag="VN084_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28958.1"
                     /translation="HLTARCLDVVVPIAGKRMTPWFEPLRLELLAASMEHAFPALLWP
                     VKRWLCPTSWGAFTLLRGSPTEAGSNAPPLVRQALQLAPLVSDFLDVWRAALRFPVTY
                     APMRWRGPTILPPQAAVHAFRMIRDAQNSRLRLSADIIDLCMHRVFIHPHLPRHPEIQ
                     DDIQAAVAGQSTLQARFDNYDDFAWQRIVASLPPAANYT"
     gene            599..>3118
                     /locus_tag="VN084_05565"
     CDS             599..>3118
                     /locus_tag="VN084_05565"
                     /inference="COORDINATES: protein motif:HMM:NF041559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector BTH_I2691 family protein"
                     /protein_id="HWT28959.1"
                     /translation="MSISKTLTQARRQAAPNPTGTCSACERTGLPILPLRLAVMPLTD
                     GLKQHGALSHESIQTALRVLRQGYVYVLLDKEIWHAYQVTPEGYLRQFNPFSMPRAAP
                     EPLANACLDAGHDLRSAFINIDTAAHKQAWIAFSQDPWPKATLDAYRTGKHADGRALP
                     ADYRQRFRVINLITLKQDPTGGDQALALEHGDDLAQHVAEYAIGTRDFGSVHDWHPRH
                     ARLEAMHAQLRMLEKQHGLAKGVAGLILPDPVGMAAELNSLRLSVNTMRQRWREEPTR
                     RYAYLTSQCLLGIKAYIAQQVAAAHPPPPPPGDYWTSSESGVPPVFHDPAQQRAGVVN
                     CETGRRMARLEDRYWETGPAGRKTFQDAYDKEDDRFQKQIDAYATDWADLVAGASWRR
                     IMALDYADADARSQQCRLSTTAACLAGGITDAPLQHTAPADESQPGEKPPPQALGPSS
                     KAWKDLLSDAGSPAYIALNGQYTDLQQAFEPLFSNAMPSEAGKKYYDAIKMIATSKEG
                     SDWRRTLVVESADQLLAAIHDASNRLENTLSIGAKAALDNLQSGAAWLYGKAKFTQVT
                     IQLTLGECFELLSSEIREHANSAQKAAGKRARAMLLGSLITIPNANVRNVLIDVTLWA
                     HGTAEEVKKRILDFSREAKEGAQDIRQAAGALGQEINREAKSALRQVNAGLKNLEPAA
                     AAVLEGLQVGAAGARRVAGSTFASLRSLAVGSVDGGLSFIGLYFLQDSLKSTLADLDA
                     KVGAKHPEAVAAFYSVALGFMGASVEAAGLAMRVPADAARGFVLRWGVEKTPVLTRVV
                     GLGSKMVLVGGAISALAGIADAGASFRAAARVSKAGDVGAAK"
BASE COUNT          613 a          980 c          957 g          568 t
ORIGIN      
        1 atcacttgac tgcgcgctgc ctggatgtcg tcgtgcctat agctggaaaa cgcatgacgc
       61 cgtggttcga gccgttgcgg cttgaactgc tcgcggcatc aatggaacac gcctttcctg
      121 ctctgctgtg gcccgtcaaa cgttggcttt gcccgaccag ctggggcgcc ttcaccttac
      181 ttcgcggctc ccccaccgaa gccggctcga atgcccctcc actcgttcga caggcactgc
      241 aacttgcacc gctcgtcagc gatttcctgg atgtctggcg cgccgcgctc cgattccccg
      301 tgacgtatgc cccaatgcgc tggcgtggcc ccacgattct tccgccccag gccgccgtgc
      361 acgctttccg gatgatccgt gacgcacaga actcacgctt gcgcctgagc gccgacatca
      421 tcgacctgtg catgcatcgc gtcttcattc atccccatct gccacggcat ccagaaatac
      481 aggacgacat ccaagccgcc gtcgcagggc agtccacatt gcaagcgcgt tttgacaact
      541 acgacgactt tgcgtggcag cgcatcgttg cgtcattacc tcccgccgcg aactacacat
      601 gagcatcagc aagaccctga cccaggcacg gcgccaggcg gcgcccaatc ccaccggcac
      661 gtgcagcgcg tgtgaacgta ccggcttacc gatcctgccg ctacggcttg cagtcatgcc
      721 gctcacagac ggactcaagc aacatggcgc actgagtcac gaatccattc agaccgcatt
      781 gcgtgtattg cggcaggggt atgtctatgt gctgctggac aaagaaatct ggcatgccta
      841 tcaggtcacc ccggaaggct atctgcgcca gttcaatcca ttctccatgc cacgggccgc
      901 gcccgagccc ctagccaacg cgtgcctgga cgccggacac gatctgcgct cggcattcat
      961 caacatcgac accgcagcgc acaagcaggc ctggatcgcg ttttcacagg acccttggcc
     1021 gaaggcgaca ctcgatgcct accgcaccgg caagcacgct gatggaaggg cactgcccgc
     1081 tgattatcgg cagcggttca gggtgatcaa tctgatcacg ctcaagcagg atccgacagg
     1141 tggcgaccag gcgcttgccc ttgagcacgg tgatgatctt gcccagcatg tcgccgagta
     1201 cgccatcggc acgcgcgact tcggtagcgt tcatgactgg catccccgtc atgcccgcct
     1261 tgaagccatg catgcgcaat tgcgcatgct cgaaaagcag catgggcttg cgaagggggt
     1321 tgcaggactc atcctgcccg acccggtagg catggccgca gagctcaaca gccttcggct
     1381 gtctgtcaac acgatgcgcc agcggtggcg tgaagagccg acaaggcggt acgcctacct
     1441 cacctcgcag tgcctgctcg gcatcaaggc ttacatcgcc cagcaggtag cggctgcaca
     1501 cccgccgccg ccgccacctg gcgactactg gacgtccagc gaatccggcg tcccgcccgt
     1561 attccatgac cccgcgcaac aacgtgctgg cgtggtcaat tgcgagaccg ggcgtcgcat
     1621 ggcccggcta gaagatcgtt actgggaaac tggccctgca gggcgaaaga cttttcagga
     1681 tgcgtacgac aaggaagacg accggtttca aaagcagatc gacgcttatg caaccgactg
     1741 ggccgacctg gtggctggcg ccagttggcg acggatcatg gctttggact atgcggacgc
     1801 cgatgcgcgt tcgcagcaat gccgcctgag caccaccgcg gcttgtctcg cgggcggcat
     1861 cacggatgcc ccgctacagc acacagcgcc ggcggacgag agccaaccag gcgaaaagcc
     1921 cccgccgcaa gcgttggggc ctagcagcaa ggcatggaaa gacctgctgt ccgacgcagg
     1981 tagtccggcc tacatcgcgc tcaatgggca atacaccgac ttgcagcagg cgttcgaacc
     2041 cttgttctcc aacgcgatgc cctcggaggc aggcaagaaa tactatgacg cgataaagat
     2101 gatcgccacg agcaaggagg gaagcgattg gcgccggacg ctcgttgtcg aatctgccga
     2161 tcagttgttg gcggccattc acgatgcgag caatcgcctc gaaaacaccc tgagcatcgg
     2221 cgccaaggcg gctttagaca acctccagtc cggcgccgcc tggctgtacg gtaaggccaa
     2281 gttcacccag gtcaccatcc agctcacgtt gggggagtgc ttcgagctgc tgtcgagcga
     2341 gattcgcgag cacgccaact ccgcccaaaa ggccgccggc aaacgggcac gggcgatgtt
     2401 gctgggcagt ctgatcacca tcccgaatgc caatgtccgc aatgtgctca tcgacgtcac
     2461 gctatgggcg catggaactg cagaagaggt caagaagcgc atcctggatt tcagccgcga
     2521 agccaaggag ggcgcgcaag acatcaggca ggctgctggc gcgcttggac aagagatcaa
     2581 cagagaagcc aagtctgcgc tacgccaggt caacgccggc ctgaagaatc tggaacccgc
     2641 tgccgccgcg gtgctcgagg gcctgcaagt tggcgcggcg ggggctcgtc gtgtcgccgg
     2701 cagcaccttt gcaagcctga ggagcctagc cgtcggtagc gtggatggcg gcttgtcttt
     2761 catcggcctg tactttctgc aggacagctt gaaaagcacg ttagctgacc tggatgcgaa
     2821 agtcggggca aagcatccgg aggccgtggc ggcgttttat agcgtggcgc tgggcttcat
     2881 gggagcgagt gttgaggcgg ccgggctggc gatgagggtt ccggcggacg ctgcccgagg
     2941 gttcgtgctg cgctggggtg tggagaaaac tcctgtcctc acgcgcgttg tcgggttggg
     3001 gtcgaagatg gtgctggtgg ggggagcgat ttcggccttg gcggggattg cggatgctgg
     3061 tgctagtttt cgtgctgccg ctcgcgtgtc aaaagctggt gatgtagggg ctgcaaag
//
LOCUS       DATMTW010000286         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_158162, whole genome shotgun sequence.
ACCESSION   DATMTW010000286 DATMTW010000000
VERSION     DATMTW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158162"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VN084_05570"
     CDS             <1..712
                     /locus_tag="VN084_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT28960.1"
                     /translation="VSIDLHAGTTLAVVGESGSGKSTLARCLLQLLPLDSGEVLFQGV
                     DLAKLTAAELRQRRRDMQMVFQDPFASLNPRMRIGEIVAEGLLIHGLGNADERRQKAL
                     GMLKRVGLSEADMNKYPHQFSGGQRQRIGIARALVLEPKLVVCDEPVSALDVSIQAQI
                     LLLLKELQQEMALSYLFISHDLRVVRHIADEVAVMHHGKLIEHGQVEALYAAPQQEYT
                     KNLLSAIPGRKGMAAQTA"
     gene            731..1357
                     /locus_tag="VN084_05575"
     CDS             731..1357
                     /locus_tag="VN084_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT28961.1"
                     /translation="MAYDLEEQEQLDAIRAWWRTNGNKVLSVIAVAAVVYAGIQGWKI
                     YQHKQAAGASAEYQTLTKTDIKDTKAIKTIAGDLMDNYRMTPYAGRAALLAAKANYQD
                     KDTKTATAQLEWAIKNAKEDAVKALAALQLAAIQYETKAYDAALKTLSEKHDAAFDGL
                     FADLRGDILMAQGKKVEAKSAYVEALAKLDPQGRYHAYTQHKLEALGS"
     gene            1374..2543
                     /gene="bamB"
                     /locus_tag="VN084_05580"
     CDS             1374..2543
                     /gene="bamB"
                     /locus_tag="VN084_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HWT28962.1"
                     /translation="MKLRTTLLALLALMLCACSSLFDLKNDMSERMFGRDLAEPPQEL
                     AQFKPTMTPKIVWHTNLGESQEFDFTPAVDGGMVYAASASGEIVKVDAATGKEVWRVN
                     AGEPLSGGVGLGPNLVLVGTPKAYVMAYDQSGKLLWKSKVSSEVLSIPQVSDDLVIVR
                     AGDSRIFGISAKDGSRKWVYERVNPPLSLRSSAGVVLADGAVYAGFAGGKMIALRAED
                     GKIIWEVSVALPKGTTEIERIADITSQPFVDGPLVYAVAYQGKIAGVDRATGKVVWNR
                     DISSYTGLSAEDARVYVSHAVGAVYALEYSNGKTFWRQGALKNRLLSAPLALGDVIAV
                     GDVQGYVHFLSREDGAFAARINVQDSPIMPRLTGLGSNGLLAQTRKGGLYALSTK"
     gene            2547..>3046
                     /locus_tag="VN084_05585"
     CDS             2547..>3046
                     /locus_tag="VN084_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWT28963.1"
                     /translation="MIPTIVLVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGR
                     GIGGSRPYLVVDTGGFEPAADTGILKAMARQTLLAIDEADVIIFLVDGRQGVSPQDHV
                     IADRLRKSQRPVLLAVNKTEGIQRAIASAEFHELGLGEPLSISSAHGEGVRDLVELAL
                     DNVPAE"
BASE COUNT          660 a          856 c          934 g          596 t
ORIGIN      
        1 tgtcagtatc gatctacatg cgggtacgac tctggctgtg gtgggcgagt cgggaagcgg
       61 caagtctacg ctggcccgtt gtctgctaca attgctgccc ttggatagcg gcgaagtgct
      121 tttccagggg gtcgacctgg caaagttgac ggccgcggag ttgcggcagc ggcgacgcga
      181 tatgcagatg gtgttccagg atcctttcgc ctcgctcaat ccacgcatgc gcatcggcga
      241 gatcgttgcc gaaggcttgc tgatccatgg actgggcaat gctgacgaac gtcggcaaaa
      301 agcactcggc atgcttaaac gcgtcggttt gagcgaagcc gatatgaaca aatatcccca
      361 tcagttctcg ggcgggcagc ggcagcgcat cggtatcgcg cgtgcgctgg tactggaacc
      421 caagctggtg gtgtgtgacg agccggtatc ggcgctggac gtgtcgatac aggcgcagat
      481 cctcttgttg ctgaaggagt tgcagcaaga gatggcattg agttatctct ttataagtca
      541 cgatctgcgc gtcgtcaggc atatcgcgga tgaagtggcg gtgatgcatc atggcaagct
      601 gatcgagcac ggccaggttg aagcgctcta cgcagcgccg caacaggaat atacgaagaa
      661 tctattgagc gcgatcccgg gccgaaaagg catggccgcg caaacagcat aaacgagact
      721 aggaacgggt atggcatacg atttggaaga acaggaacag ttagacgcga tcagagcgtg
      781 gtggcggacc aatggcaaca aggtgttgtc ggtgatcgcg gtcgcagctg ttgtctatgc
      841 gggcatccag ggctggaaga tctaccaaca caagcaggct gcaggcgcat ccgccgaata
      901 ccagaccttg accaagacgg acatcaagga caccaaagcg atcaagacga tcgcaggcga
      961 tctcatggat aactaccgca tgactccgta tgccggtcgt gcggccctgc tggcggctaa
     1021 agccaactat caggacaagg acaccaaaac ggcgaccgca cagctcgaat gggctatcaa
     1081 gaacgccaag gaagatgcgg tgaaggcact tgcggctcta cagctggcag ccatccagta
     1141 tgagaccaag gcctacgatg ctgcgctcaa gaccttgtcc gaaaagcatg acgctgcctt
     1201 cgacggcctg tttgccgacc ttagaggcga tatcctcatg gcgcagggca aaaaggtcga
     1261 ggccaagagc gcttatgtcg aagcgcttgc caaactggat ccgcaaggcc gttaccatgc
     1321 ctatacccag cacaagctgg aagccttggg cagttaaatt gcctgtagtg aacatgaaac
     1381 tacgaaccac actcctggca ctgctcgcgc tcatgctctg cgcctgcagt tcgctctttg
     1441 acctgaagaa tgacatgtcc gaacgcatgt tcggccgtga tctcgccgag ccgccgcagg
     1501 agcttgcaca gttcaagccg accatgaccc caaagatcgt ctggcatacg aatctgggag
     1561 aatcacagga atttgatttt accccggctg ttgacggcgg catggtctac gcggccagcg
     1621 caagtggcga gatcgtcaag gtcgacgcag ccaccggcaa ggaagtgtgg cgtgtcaacg
     1681 ccggtgagcc gctatccggc ggtgtcggcc ttggccccaa tcttgttttg gtgggcacac
     1741 cgaaggctta cgtcatggcc tacgaccaat caggcaagct gctgtggaaa tccaaggtga
     1801 gcagcgaagt gctcagcatc ccccaggtca gtgacgacct tgtcatcgtt cgtgcgggcg
     1861 acagccgtat ctttggtatc agtgccaagg acggcagtcg taaatgggtc tacgaacgcg
     1921 tgaatccgcc actgagccta cgtagctcgg caggcgtggt gctggctgac ggcgccgtct
     1981 atgcaggttt tgccggcggc aagatgatcg ccctgcgtgc ggaagacggc aagatcatct
     2041 gggaagtgtc ggtggctctg cccaaaggta cgaccgagat cgaacgcatc gccgatataa
     2101 ctagccagcc attcgtcgat ggcccgctgg tttatgccgt cgcctatcag ggcaagatcg
     2161 ccggtgtcga ccgcgctacc ggcaaagtcg tctggaaccg cgatatctcc agctacactg
     2221 gcctcagcgc agaggatgcg cgcgtttacg tatcgcatgc cgtcggcgcg gtctacgcac
     2281 tcgaatactc caacggcaag accttctggc gccagggtgc gcttaagaat cgcctgttaa
     2341 gcgcaccgtt ggcactaggg gatgtgattg ccgtcggcga cgtgcagggt tatgtccatt
     2401 tcctctcgcg agaagacggc gcctttgccg cacgcatcaa tgtacaggat agtccgatca
     2461 tgccccggct caccgggctg ggcagcaacg gattgctggc gcagacccgc aagggcggcc
     2521 tgtacgcgct ttcgaccaaa taacgcatga ttcctaccat cgtccttgtc ggccggccga
     2581 atgtcggaaa gtcgaccctg ttcaatcgcc tgactcgtag tcgggatgca ctggtcgctg
     2641 acttgccggg tttgacgcgt gaccgccatt acggtcgcgg catcgggggc agtcgcccct
     2701 acctcgtggt cgataccggt ggcttcgagc cagccgcaga taccggtatc ctgaaggcga
     2761 tggcacgtca gactttgctc gcgatcgacg aagcggatgt catcatcttt ctggttgacg
     2821 gacgccaagg tgtctcgcct caggaccatg tgatcgctga ccggctgcgc aagagccagc
     2881 gcccggtatt gctggcggtg aacaaaaccg aaggtatcca gcgcgcgatc gccagcgccg
     2941 agttccatga gcttggtctg ggtgaaccgc tgtccatctc ctcagcacac ggcgaggggg
     3001 tgcgcgacct ggtcgagctg gccctcgata acgtgccggc agagat
//
LOCUS       DATMTW010000287         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_461530, whole genome shotgun sequence.
ACCESSION   DATMTW010000287 DATMTW010000000
VERSION     DATMTW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461530"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VN084_05590"
     CDS             <1..576
                     /locus_tag="VN084_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HWT28964.1"
                     /translation="TFIEVYLEDYHVEPFHPGLGNFVTCDDLRWEFGEHYSVQTVGVR
                     SLEKHGSPVYQTWQQQLTQYMQGRQPKFGAIWMVYYPYLMIEWYPNVLVVSHLIPRGV
                     DACTNVVEFYYPEDIALFEREFVEAQQAAYQETALEDEEICRRMHQGRRALHAQGIDE
                     RGPYQHPMETGLEHFHLWLRNRLEPHLKRPY"
     gene            736..1428
                     /locus_tag="VN084_05595"
     CDS             736..1428
                     /locus_tag="VN084_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28965.1"
                     /translation="MVTIAHTYRWLLLALASLSLGGCFSGSLAAQIASSMATHIADKA
                     ISTSLDAQQRAEDAKGIALEDTTPDEYWAAFLTSGFSRVAPTAETLPPDYRQAAYTPP
                     HASANILASRLLKIETWNVVIGEEKTRILENARRVGATLPDKLDWPNWQLASGPIDAS
                     GKKTIYFLVSPDVGKVRTGSRVVVEISEVGGLHMARYLLDPVSTQAATPVKDRSDPNA
                     SQSARFQLSYSR"
     gene            complement(1364..>1938)
                     /locus_tag="VN084_05600"
     CDS             complement(1364..>1938)
                     /locus_tag="VN084_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWT28966.1"
                     /translation="LESASDIKVIAEAENGEQAVKLYMEHKPDVVVMDITMPGIGGLE
                     AIERIMAKDNTARVLVLSAHEDSVHPKRVLNAGAMGYLTKRSAAEELIKAIRTVAGRK
                     MYLEASIAQQMAIQQLSGEQNPVDVLSDREFEVFMALAKGKTTNDIAETLCLSPRTVG
                     THLYNIKQKLNASNSAEIALIAMRSGLIDP"
BASE COUNT          415 a          616 c          533 g          374 t
ORIGIN      
        1 accttcatcg aagtctatct ggaggattac cacgtcgagc cctttcatcc gggcctgggc
       61 aacttcgtca cctgcgacga tctgcgctgg gaattcggcg agcactacag cgtgcagacc
      121 gtgggcgtga gatcgctgga gaagcatggc tcgccggtct accagacctg gcagcaacag
      181 ttgacgcaat acatgcaagg ccggcaaccg aaattcggtg ccatctggat ggtctactac
      241 ccctacctca tgatcgagtg gtaccccaac gtgctggtgg tctcgcacct gattccgcgc
      301 ggcgtcgacg catgcaccaa tgtcgtggag ttctactacc cggaagacat cgcgctgttc
      361 gaacgcgaat tcgtcgaagc gcagcaggct gcgtatcaag agacggcact cgaagacgaa
      421 gagatctgtc gccgcatgca tcaaggacgc agggcactgc atgcacaagg tatcgacgag
      481 cgcggacctt accagcaccc gatggaaacc gggctggaac acttccacct gtggctgcgc
      541 aaccgcttag aaccgcacct gaaaagaccc tactagcccg tctggcggca accgcaagcg
      601 ctcgcaatgc caatggtcag gacggcagcg aacgaatcaa aacaatcact tgggctggac
      661 aaacgtattt cgcagggata cgcttacctt cccgcgttag aaacgtatcg atgccgacga
      721 cgaatcatcc aggccatggt gacgattgcc catacatacc gctggctatt gctggcgcta
      781 gcaagccttt ccctcggcgg atgcttcagc ggcagcctgg cggcgcagat cgcctcatcg
      841 atggccaccc atatcgcgga caaggcgatc tccacatcgc tcgatgcaca gcaaagagcc
      901 gaagacgcca aggggatcgc gctcgaggat acgacgccgg atgaatattg ggcagcgttc
      961 ctgacctccg gcttctccag agttgcgcct accgccgaaa cactgccgcc agactaccgc
     1021 caagccgcct acaccccccc gcatgcatcc gccaacatcc tggcctcccg cctgctgaag
     1081 atcgagacat ggaacgtcgt catcggtgaa gagaagaccc gtatcctgga aaacgcccgg
     1141 cgtgtcggcg ccaccctgcc agacaagctc gactggccga actggcagct ggcgagtggc
     1201 ccgatcgacg cgtccggcaa aaagaccatc tatttcctgg tatcgcccga tgtgggcaag
     1261 gtccgcaccg gctcacgcgt tgtggtcgag atatcggaag tcggcggcct gcatatggcc
     1321 cgctacctcc tggacccggt atccacgcag gcagcaacgc ctgttaagga tcgatcagac
     1381 ccgaacgcat cgcaatcagc gcgatttcag ctgagttact cgcgttgagt ttttgcttga
     1441 tgttgtagag atgggtaccg acggttcgcg gcgagaggca caatgtctcc gcgatatcgt
     1501 tggtggtctt gcctttggcc agggccatga agacttcgaa ttcccggtcc gatagcacgt
     1561 ccaccggatt ctgctcgccc gacaactgct ggatcgccat ctgctgtgcg atgctggctt
     1621 ccagatacat cttgcgcccg gccacggtac ggatcgcctt gatcagttct tccgccgcgc
     1681 tacgtttggt caggtaaccc atggccccgg cattcaagac gcgcttggga tgcaccgagt
     1741 cctcatgcgc agacaacacc agcacccgcg cggtattgtc cttggccatg atgcgctcga
     1801 tagcttccag cccgccgatc ccaggcatgg tgatatccat gaccaccaca tccggtttgt
     1861 gctccatgta caacttgacg gcttgctccc cattctcggc ctcggcgatc accttgatat
     1921 ccgaagccga ttcgagca
//
LOCUS       DATMTW010000288         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_226959, whole genome shotgun sequence.
ACCESSION   DATMTW010000288 DATMTW010000000
VERSION     DATMTW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226959"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="VN084_05605"
     CDS             complement(<1..1474)
                     /locus_tag="VN084_05605"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWT28967.1"
                     /translation="MSQVALPPNAQDLHWLLVSVGLVMALVLPYLSPWIAVFIAVFFG
                     WRYLVERNAWHLPSLWLLLPITVLGGIGVMITYRGFIGRDASISLLVIMLALKLMETR
                     SKRDFTLVVFIGYFTAITAFLFDQTMLIGAFLLLPLLALTITLIGINHPAGTLDWRFK
                     FKLARNLLLQSVPMMLLLFLLFPRIPGPLWGVPKDAYEGMTGLSDSMEPGNISQLSQS
                     GKTAFRVQFEGKIPPNNALYWRGPVLWHFDGRKWSMSSPDLPIGQESLALQGDPVRYS
                     LTLEPHNRNWLLMLEMPGALPLNAVQTRDMQVLSKRPVRTRIRYDGTAYFNYRLGETL
                     PERTRELTLQIHDGENPKTAALAQRWRDEGKSPQAMIDAALQMFRNERFFYTLSPPLL
                     GSNPIDDFLFNTRRGFCEHYASSFVYLMRAAGVPARVVTGYQGGEVNPVGNYLIVRQS
                     DAHAWAEVWLAGKGWTRVDPTAAVAPQRVNAGIDAALPDNA"
     gene            complement(1471..2427)
                     /locus_tag="VN084_05610"
     CDS             complement(1471..2427)
                     /locus_tag="VN084_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWT28968.1"
                     /translation="MAAILNIPDWRKWLRVARADHAPAVLTRRHIYILPTRYGLLYGV
                     ILIGMLIGSINYTLSLGFFLTFLLAGMGNIAMLHTWRNLANLRIRHHRADPVFAGEAA
                     IFELMVAETQLRARHAIGAHFDDMPPVHIDIPAGSEQPIRLALTSKRRGWLNAGRITF
                     FTEFPLGLFHVWSYVTIDMRCLVYPHPASVSAALPESADMGATGSLDRSSGDDDFAGH
                     RNYQLGDSPRRVDWKASSREQGLLTKQFQGEGQSTLWLDWAQTAGADGEQRIRQLTRW
                     VVDAQAAHKSYGLRLPAVSISPGNSEAHYHRCLEALALMELQ"
     gene            complement(2443..3354)
                     /locus_tag="VN084_05615"
     CDS             complement(2443..3354)
                     /locus_tag="VN084_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWT28969.1"
                     /translation="MHNVTQSIIAAASRVILGKEPQIRMALACILARGHLLIEDLPGM
                     GKTTLAHTLAQVLGLEFRRVQFTSDLLPADILGVSVYDRNSASFKFHPGPVFTNVLLA
                     DEVNRATPKTQSALLEVMEEGQVTLEGETRKLPQPFFVIATQNPTHHIGTFPLPESQL
                     DRFLMRIHLGYPDVRSERELLKHNGGRAAIDDLPPCIDGAGLIALQRAVDGIHVSDAL
                     LDYIQALLDYSRNAARYSAGLSPRAGLALKQCAQAWAMMQGRDHALPEDVQAILPGIA
                     GHRLQAASGMEGTGDVSAHLLSAVAIP"
     gene            complement(3366..3818)
                     /locus_tag="VN084_05620"
     CDS             complement(3366..3818)
                     /locus_tag="VN084_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWT28970.1"
                     /translation="MPRELHGINRFAVRAMIWLNGFAHIIIGLALAIAVLMFTWLFFQ
                     DVRQAAGAHNLVHGFLHALGTLMLLWSVSALITAEIRYLLGSQLEVEAFIEVAMIVVL
                     RKLIVMPVEDATPLPTDVALWVGAALMLGIMYVLVRWSDRQTIAAIKK"
BASE COUNT          708 a         1109 c         1326 g          676 t
ORIGIN      
        1 tcgcgttatc cggcagcgcc gcgtcgatgc cggcattgac gcgttggggc gccacggcgg
       61 cagtcgggtc gacccgcgtc cagcccttgc ccgcaagcca cacttcggcc caggcgtggg
      121 catcggattg cctgacgatc aggtaattgc cgaccggatt gacctcgccg ccctggtagc
      181 cggtgaccac gcgggcaggg acgccggctg cgcgcatcag gtagacgaag ctgctggcgt
      241 aatgttcgca gaagccgcgc cgggtgttga acaggaaatc gtcgatcggg ttgctgccca
      301 atagcggcgg tgaaagcgta tagaagaagc gctcgttacg gaacatctgc agggccgcgt
      361 cgatcatcgc ctgcggcgat ttgccctcgt cgcgccagcg ttgcgccagg gctgcagtct
      421 tcgggttctc cccgtcatgg atctgcaagg tcagttcccg cgtccgttca ggcagtgtct
      481 cgcctagccg gtaattgaaa taggcggtgc cgtcgtagcg gatacgggtg cgtaccggcc
      541 gtttcgatag cacctgcatg tcgcgcgtct gtacggcgtt gaggggcagc gcgcccggca
      601 tctccagcat cagcagccag ttgcggttgt gcggttccag cgtcaggcta taacgcaccg
      661 gatcgccctg cagggccagt gactcctgac cgataggcag atccgggcta ctcatgctcc
      721 atttgcggcc gtcaaaatgc cagagcaccg gcccgcgcca gtagagtgcg ttgttgggcg
      781 ggatcttgcc ctcgaactgc acacggaacg ctgtcttgcc ggattggctc agctggctga
      841 tgttgcccgg ttccatcgaa tccgagagcc cggtcatgcc ttcataggcg tccttgggca
      901 caccccacaa ggggccggga atgcgtggga agagcaagaa cagcaatagc atcatcggca
      961 cagattgcag caagaggttg cgtgccagct tgaatttgaa gcgccagtca agcgtcccgg
     1021 cagggtggtt gataccgatc agcgtgatgg tgagcgccag caaggggagc agcaggaaag
     1081 cgccgatcag catggtctgg tcgaacagga aggcggtgat ggcggtgaag tagccgatga
     1141 acaccaccag cgtgaaatca cgtttgctgc gcgtttccat gagtttcagc gcgagcatga
     1201 tcaccagtag cgagatgctg gcgtcgcggc cgatgaagcc gcgataagtg atcatgacgc
     1261 cgatgccgcc gagcacggtg atcggcagca gtagccaaag cgaaggcagg tgccaggcgt
     1321 tgcgctcgac caggtagcgc cagccgaaga atacggcgat gaagaccgcg atccagggcg
     1381 acaggtaggg cagcaccagc gccatgacca ggccgacgga gaccagcagc cagtgcaggt
     1441 cctgggcgtt gggcggcagc gcgacctggc tcactgtagc tccatcagtg ccagtgcttc
     1501 caggcatcgg tggtaatggg cctcgctatt gcccggactg atgcttacgg caggcaggcg
     1561 gagaccgtag cttttgtggg cagcctgggc atccactacc cagcgcgtca gttggcggat
     1621 gcgttgctcg ccgtctgcgc ccgcggtctg tgcccagtcg agccacagcg tgctctggcc
     1681 ttcgccctgg aactgtttgg tgagcaggcc ttgttcgcgt gaggaggcct tccagtcgac
     1741 gcgccgtggc gagtcgccga gctggtagtt gcggtggccg gcgaaatcgt catcaccgct
     1801 ggagcgatcg agcgagccgg tggcccccat gtcggccgat tcaggcaggg ctgcggatac
     1861 gctggcaggg tgcgggtaga cgaggcaacg catgtcgatc gtgacatacg accagacatg
     1921 gaacagcccc agcgggaact cggtgaagaa cgtgatgcgc ccggcgttga gccatccgcg
     1981 ccgcttgctg gtcagggcga gccggatcgg ctgttcgctg ccggcgggga tgtcgatatg
     2041 gacgggcggc atatcgtcga aatgcgcgcc gatcgcatgc cgcgcgcgca attgcgtttc
     2101 ggcgaccatg agttcgaaga tcgcagcttc gccagcgaac acggggtcgg cgcgatggtg
     2161 gcggatacgc aggttggcca ggttacgcca ggtatgcaac atggcgatgt tgcccatgcc
     2221 ggcgagcagg aacgtcagga agaaccccag gctcagcgtg tagttgatcg agccgatcag
     2281 catgccgatg aggatgacgc cgtagaggag gccatagcgc gtcggcagga tgtagatatg
     2341 gcgacgcgtc agcaccgcgg gggcatgatc ggcacgcgcg acacgcagcc acttgcgcca
     2401 gtcagggatg ttcaggatgg cggccatgcc aggctcaagc catcagggga tggcgaccgc
     2461 actcagcaag tgtgccgata cgtcgcctgt gccttccatg ccgctcgcgg cctgcaggcg
     2521 gtggccggcg atgcccggca atatcgcttg cacgtcctct ggcagcgcat gatcgcggcc
     2581 ctgcatcatg gcccaggctt gcgcgcattg cttgagggcg agcccggcgc ggggcgaaag
     2641 gcctgccgag tagcgggcgg cgttgcgtga gtagtcgagc agcgcctgga tgtaatcgag
     2701 caaggcatcg gagacatgga taccgtccac cgcccgttgc agggcgatga gtccggcacc
     2761 gtcgatgcag ggtggcagat cgtcgatggc ggcgcggcca ccattgtgct tcagcagttc
     2821 gcgttccgag cgcacgtcag ggtagccaag atggatacgc atgaggaaac ggtcgagctg
     2881 cgactcgggt aaggggaaag tgccgatgtg gtgtgtcggg ttctgggtgg cgatgacgaa
     2941 gaacggctgc ggcagcttgc gcgtctcgcc ttcgagcgtg acctggcctt cttccatgac
     3001 ttcgagcagc gcgctttggg tcttgggcgt ggcgcggttg acttcgtcgg ccagcagcac
     3061 gttggtgaac acaggccccg gatggaactt gaaactggcg ctgttgcggt cgtagaccga
     3121 gacgccgagg atatcggccg gcagcaggtc gctggtgaac tgcacgcgac ggaattccag
     3181 gcccagtacc tgcgccagcg tatgcgccag cgtggtcttg cccatgcccg gcaggtcctc
     3241 gatcaggaga tggccgcgcg ccaggataca ggccagggcc atgcggatct gcggctcctt
     3301 gccgaggatg acccggctcg ctgcggcgat gatggattgt gtgacgttat gcataggggg
     3361 cggtttcact tcttgatggc ggcgatggtc tgacggtctg accatctgac cagtacgtac
     3421 atgatgccga gcatgagcgc ggcgccgacc catagcgcga cgtcggtcgg caacggcgtc
     3481 gcgtcttcga ccggcatcac gatcagcttg cgcaagacga cgatcatggc gacttcgatg
     3541 aaggcctcga cttcgagctg gctgcccagc aggtagcgga tctccgccgt gatcagcgcc
     3601 gacaccgacc atagcagcat cagcgtgccg agtgcgtgca ggaagccatg gacgaggttg
     3661 tgcgcccccg ccgcctggcg tacgtcctgg aagaacagcc aggtgaacat gagtaccgcg
     3721 atcgccagcg ccagcccgat gatgatatgg gcgaagccgt tgagccagat catggcgcgc
     3781 acggcgaaac ggttgatgcc atggagttca cggggaatt
//
LOCUS       DATMTW010000289         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_629732, whole genome shotgun sequence.
ACCESSION   DATMTW010000289 DATMTW010000000
VERSION     DATMTW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629732"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1183)
                     /locus_tag="VN084_05625"
     CDS             complement(53..1183)
                     /locus_tag="VN084_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HWT28971.1"
                     /translation="MNNHPLATRLRAASMVAILASASVLGACTKEPETPPEPAPAPEA
                     STMKHDNVAYVTNQDGGVTVIDLATMETVSDIDVKGEGPRGVGVTDDGKLLITANKDN
                     GNISVIDTATGKLVKHIEVGKNPEFVRVKGDYAFVSFEPSSKGGPPPKPGTVVKEEED
                     DDENAEPARIAVVDLKAGEKIREIVGGPETEGIEFSKDGKHLIITNEADNTITVHNME
                     TGELEQTISTKDYGLRPRGIKASPDGSFYVATLEFGNSLLVLDSDFKPVKAVKTGLSP
                     YGVSFDRDGKRIYVAANKAKTMEVYDAKTFDKIKEIPTGNRCWHFTFTPDDKQILLAC
                     GKSDEVLVIDTDKLEVTKHIGDKKMPWGLVTYPKSMGSLDQP"
     gene            complement(1426..1731)
                     /locus_tag="VN084_05630"
     CDS             complement(1426..1731)
                     /locus_tag="VN084_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT28972.1"
                     /translation="MGKLHLRHLVGGHAPQAEHEQVYRFEFPEKPGALMKFLDTMGHD
                     WNISLFHYRNHGADYGRVLVGMQVPPAEKVEFKTFLDQLGYPYWDESDNPAYRLFLG"
     gene            complement(1726..3219)
                     /gene="ilvA"
                     /locus_tag="VN084_05635"
                     /pseudo
     CDS             complement(1726..3219)
                     /gene="ilvA"
                     /locus_tag="VN084_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
     gene            3290..>3562
                     /locus_tag="VN084_05640"
     CDS             3290..>3562
                     /locus_tag="VN084_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HWT28973.1"
                     /translation="MATQDELKQQVAEAAVAYVKGGIIGVGTGSTANYFIDALAKVKG
                     KIDGAVASSEATAERLRAHGIKVYDMNQVDSLDIYVDGADEITEHMH"
BASE COUNT          676 a          977 c         1088 g          821 t
ORIGIN      
        1 cggccctgat gggccgtttt tcattggtgc acgctaatgc ttacaagtgc gcttaaggtt
       61 ggtccaaact acccatggat ttcggatagg tgaccagacc ccatggcatc ttcttgtcgc
      121 cgatgtgctt ggtaacttcc agcttgtcgg tgtcgatcac caggacttca tccgacttgc
      181 cgcaggccag caggatctgc ttgtcgtccg gcgtgaaggt gaagtgccag caacgattgc
      241 cggtggggat ctccttgatc ttgtcgaagg tcttggcatc gtagacttcc atggtctttg
      301 ctttgttggc cgcgacatag atgcgcttgc cgtcacggtc gaaggagaca ccgtaggggc
      361 tcaggccagt cttcacggct ttcactggct tgaagtcgct gtcgaggacc agaaggctgt
      421 tgccgaattc cagcgtggcg acgtagaagc tgccatctgg cgaagccttg atgccgcgtg
      481 ggcgcaagcc gtagtccttg gtggagatgg tttgttccag ttcaccggtc tccatgttgt
      541 gcacggtgat ggtgttgtct gcctcattgg tgatgatgag gtgcttgcca tccttggaga
      601 attcgatgcc ttcggtctct gggccaccga caatctcgcg gatcttttcg ccagctttca
      661 gatcgacgac ggcgatacgt gccggttcgg cgttttcatc gtcatcctct tcttccttga
      721 ccacggtgcc tggcttcggt ggcgggccac ccttggagct gggttcgaac gagacgaagg
      781 catagtcgcc cttgacgcgc acgaactccg gattcttgcc gacttcgata tgtttcacga
      841 gtttgccggt agcggtgtcg atcaccgaga tattgccgtt gtccttgttg gcggtaatga
      901 gcagtttgcc gtcatccgtc accccgacgc cgcgcgggcc ttcacccttg acgtcgatgt
      961 cgctgacggt ttccatcgtt gccaggtcga tgactgtcac gccgccgtcc tgattggtga
     1021 cgtaagcgac gttgtcatgc ttcattgtgc tcgcctcagg ggctggtgcc ggctctggcg
     1081 gagtctccgg ttccttggta cacgcgccga gcacgctggc cgaagccagg atcgcgacca
     1141 tggacgctgc acgcaacctg gtggcgaggg ggtggttatt catcgctgtt atctcctctt
     1201 cttggtttct gaaaacgtat caaaacgaat gtgtagggtt ggtctgtctt gcgccgcata
     1261 ggttcattat gaatcgatgc cgatctgcgt cgcggtcacg tcgctacatc aagcaatatc
     1321 cgttcttgtc cacattggca aaaatatgat tgataaacag ctgcttgcat gagcggttca
     1381 aggtgggcga cggtcgctgg ctgcagtcag ccaccaactg gtggcttatc cgaggaacag
     1441 gcggtaggcg ggattgtcgc tctcatccca atacggatag ccgagctggt cgaggaaggt
     1501 cttgaattcg accttttcgg ctggcggtac ctgcatgccg accagcacac gaccataatc
     1561 ggcgccgtga ttgcggtagt ggaagaggct gatgttccag tcatggccca tggtgtcgag
     1621 gaacttcatg agcgcgcccg gcttctcggg aaattcgaag cgatagacct gctcatgctc
     1681 ggcctgcggc gcgtggccgc cgaccaggtg gcgcagatgc agcttgccca tttcgttgtc
     1741 ggtcaggtcc agcgccggaa gatcatgccg ttcgagcatt tcgaccagct tggatgattc
     1801 tccctgctgg tgcgtggtga cgccgacgaa aatatgcgct tggctcggat ccgagtaacg
     1861 atagttgaac tcggtgatgt tgcggtggcc gagcaggtta cagaatttct tgaacgagcc
     1921 cggtttttcc ggaaactcga agcggtatac ctgctcatgt tcggcttggg gggcgtggcc
     1981 gccgacgagg tggcgcagat ggagtttggc gacttcgtta tcggtgaggt cgagcgcacc
     2041 gaggccatgg tcctgcagca tatgcacgag tcgtgtggcc tcgccccgat catggatcgc
     2101 gatgccgacg aagatgtggg ccttgtgcgg gtcggaatag cggtagttga attccgtgat
     2161 gctgcggtcg ccgagtagcc ggcagaactt tttgaacgag ccgggtactt cagggatggt
     2221 cactgccaag actgcttcac gcatttcacc gatctcggcg cgttcggcga cgaaacgcag
     2281 gcggtcgaaa ttcatgttgg cgcccgaggc gatgccgacg aacgtcttgc cactgacgcg
     2341 gttacgcttg acgtattcct tgaggccggc aacggccaac gcgccggccg gttcgaggat
     2401 ggagcgtgta tcctcgaaca catccttgat cgcggcacag atggcgtcgt tatcgaccag
     2461 gatcatctca tcgacgtatt cctttgccag cgccagcgtg tgcacgccga cctgcttgac
     2521 cgcggcgccg tcagcgaaca ggccgacctg ttccagcatc acccgttccc cggtcttcaa
     2581 cgaacgtgcc atggcatcgg catccaccgc ttcgacaccg atgatcttga tctccgggcg
     2641 gacctgcttg acgtaggcgg cgatcccggc gatcaggccg ccaccgccga cacagcagaa
     2701 gatggcttcg atcggctggt gatagtcatc gaggatctcc atggcgatgg tgccctggcc
     2761 ggcgatgacg tccgggtcgt cataaggatg gacgaaggtc aggccctgcg aggcttccag
     2821 ttccagtgca tgcacatagg catccgaata cgagtcacca tacagcacga cttcagagcc
     2881 gcgggagcgc actgcgtcga tcttgatcag cggtgtggtg gttggcatga cgatgacggc
     2941 gcggcaaccg agtttttgcg cagccagcgc gaccccctgc gcatgattgc ccgccgaggc
     3001 cgcgatcacc ccgcgctgta gcgcttctgg cggcaaatgg gccatcttgt tgtaggcgcc
     3061 gcgcagcttg aatgagaaaa cgggctgcgt atcctcgcgc ttcagcagca cctgattgcc
     3121 aaggcgtgcc gagagattgg gctggagatc cagcgcggtc ttctcggcga catcatagac
     3181 gcgggcagag cggatttttt ccaggtagtc gtttttcatt tggaggctgg ggggtgtatc
     3241 attcaaaatt gcactcgatt ataaagaaaa cgtaagggtt aaagtaaaaa tggcaacaca
     3301 agacgaatta aaacaacaag tagcagaagc tgcggtcgct tacgtcaagg gtggcatcat
     3361 cggtgtcggt accggttcca ccgccaacta tttcatcgat gcgctggcta aagtcaaagg
     3421 taagatcgac ggcgctgtcg ccagttcgga agccacggcc gagcgcctgc gcgctcatgg
     3481 catcaaggtc tacgacatga accaggtcga cagcctcgat atctatgtcg atggtgccga
     3541 tgagatcacc gaacacatgc ac
//
LOCUS       DATMTW010000290         5315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_92920, whole genome shotgun sequence.
ACCESSION   DATMTW010000290 DATMTW010000000
VERSION     DATMTW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92920"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..540)
                     /locus_tag="VN084_05645"
     CDS             complement(145..540)
                     /locus_tag="VN084_05645"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HWT28974.1"
                     /translation="MLFALHLPQAQALEPRPGMWNLTATTTADGGGKPMGPFYRSQCL
                     NKEDIRDPEQLFAQSGMSDCQFKGTNQPDGRYDFTIQCGGALPMSGSGNVSYGAEQFQ
                     GEINLVTEIQGMDIATTSVVSGKRVGDCQ"
     gene            complement(572..1846)
                     /locus_tag="VN084_05650"
     CDS             complement(572..1846)
                     /locus_tag="VN084_05650"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWT28975.1"
                     /translation="MKIHIKNGRLIDPSNGIDEQRDLYIAAGKIVGLGQAPSGFVPNQ
                     VIDASGLVVCPGLVDLSARLREPGFEYKATLESEMRAANAGGVTSLACPPDTDPVLDE
                     PGLVEMLKHRAKQLNMAHVYPLGALTRQLEGQHLTEMCELTEAGCVGFSQADVAIIDT
                     QVLWRAMQYAATFGFTVWLRPEEPYLARNGVAHDGEIAARLGLRGIPAAAETLALATM
                     LRLAKETGAKLHLCRLSTAEAIDMVRNAKQEGVKLSCDISAHHLHLTEHDIGFFDANC
                     HLRPPLRSQRDRDALRMGLKDGTIDSVCSDHAPVDDDAKLLPFAEAEAGATGLELLLP
                     LTLKWAAEHKVPLVDALSRITNHPSRVLGIRAGTLSIDTDADVCIFDPDHYWKVEAKA
                     LHSQGKNTPFLGLELPGKVRYTLLHGHVVYPC"
     gene            complement(1849..2796)
                     /locus_tag="VN084_05655"
     CDS             complement(1849..2796)
                     /locus_tag="VN084_05655"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWT28976.1"
                     /translation="MHNPQLTEDGKLKHLLSIEGLPKTILNQILDTAESFVGVAEREV
                     KKVPLLRGKTVCNIFFENSTRTRTTFEIAAKRLSADVINLNVSTSSQSKGETILDTVD
                     NLTAMHADMFVVRHAQSGAAHLIARHVEPHIHVINAGDGRHAHPTQGLLDVFTIRRFK
                     PEMHNLRVAIVGDVLHSRVARSEIHALTTLGVPEVRVIAPKTLLPHDVEKLGVQVFHD
                     MEAGLRGVDVVMMLRLQNERMTGALLPSAQEYFKTYGLTPQKLALAKPDAIVMHPGPM
                     NRGVEIDSAVADGRQSVILPQVTYGIAVRMAVMSILAGN"
     gene            complement(2937..3452)
                     /gene="pyrR"
                     /locus_tag="VN084_05660"
     CDS             complement(2937..3452)
                     /gene="pyrR"
                     /locus_tag="VN084_05660"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWT28977.1"
                     /translation="MKPSDLTLPDAELLLANMAAKLKSIVQPDNALVGIHSGGVWIME
                     RLLPALGMDLAQGKLDVSFYRDDFNQRGLHHEPKPSEIPFDVEGKHIVLIDDVFYTGR
                     TIRAAMNELFDYGRPASISLAVLINRGGRELPITPQITAHDMTLPPEQNLQLQRHDDG
                     RFYLTLEESIG"
     gene            complement(3439..3933)
                     /gene="ruvX"
                     /locus_tag="VN084_05665"
     CDS             complement(3439..3933)
                     /gene="ruvX"
                     /locus_tag="VN084_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWT28978.1"
                     /translation="MTAAPDPIASAPDQPNTPALLQPQGYVLAFDFGERRIGVAIGEH
                     LLGIAHPLTTIATEANDERFRRIGELIQEWQPQRLVVGLPAHLDGEEHAMTQLCRKFA
                     RRLNGRFGLPVTLVDERLTSAEASQSLKQAGVSGRKQKVMLDQVAAQHILQSYFDSYA
                     NETQ"
     gene            complement(3938..4498)
                     /locus_tag="VN084_05670"
     CDS             complement(3938..4498)
                     /locus_tag="VN084_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWT28979.1"
                     /translation="MENVNLTGHFLIAMPAMTDPYFAKSVTFICEHNQDGAMGVVINR
                     PIDMTLDALFEQINLSLDDNPLAASPVHFGGPVQIDRGFVLHQPAGDWDSTIAINGDT
                     ALTTSKDILEAVAKGQGPDKILITLGYAGWSAGQLEQEMAQNAWLSVQASDTVIFDLP
                     SENKLHAAMNLLGLDFARLSEEAGHA"
     gene            complement(4544..>5315)
                     /locus_tag="VN084_05675"
     CDS             complement(4544..>5315)
                     /locus_tag="VN084_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWT28980.1"
                     /translation="QAAVARQASPKDIVSPGHVFPLVAENGGVLVRAGHTEAGCDLAS
                     LAGLEPAAVICEILKDDGSMARLPDLMAFASQHQLKIGTIADLIHYRSQHECLIQRAA
                     ERTVQTPYGEMQLIAYADRIAKETHLALVKGTPSADQEVLVRVHEPLSVFDLLDSTSN
                     SHSWNMLKAMQAIQRSTCGVMVLLHHNEDGADLVERILHADEPIRPRQDLRNYGIGSQ
                     ILLDLGVRKMKLMATPRKMPSMTGFGLEVTGYLEAGQA"
BASE COUNT          948 a         1528 c         1717 g         1122 t
ORIGIN      
        1 tcaatgggcg gcagaccgat tcgccgatga tatctcccaa gaggatctcg cggccttgat
       61 acgtgaacac aaggtgcata cccgcaccga gaccacttga cgtaacgggt gcccgatcag
      121 ctcaggcaaa cagcgcttga ggggtcactg acagtctccc acccgtttcc ctgaaaccac
      181 gctggtggtg gcgatatcca tgccctggat ctcagtcacc aggttgatct ctccctggaa
      241 ttgttctgcg ccatacgaca cgttccccga acctgacatg ggtagtgcgc cgccgcactg
      301 gatcgtgaag tcgtatcgcc cgtctggctg gttggtacct ttgaattggc aatcggacat
      361 gccggactgc gcgaagagtt gttccgggtc gcggatatct tccttgttca ggcattgact
      421 gcggtagaaa ggccccatgg gctttccgcc gccatcggcc gtggtcgtcg ctgtcaggtt
      481 ccacatgccc ggtctgggtt caagggcctg tgcctgcggc aggtgcagcg caaacagcat
      541 tagtaatgcc aggcgtgcgg attggcgcat atcagcacgg gtagacgaca tggccatgca
      601 ggagggtata gcgcaccttg cccggaagct ccaggcccag gaagggggtg ttctttccct
      661 ggctatgcag ggcttttgct tcgactttcc aatagtgatc cggatcgaat atgcaaacat
      721 cggcatcggt gtcgatgctc agcgtgccgg cacggatacc cagcacgcgc gatggatggt
      781 tggtgatgcg tgagagcgcg tcgaccagcg ggactttatg ctcggctgcc catttcaagg
      841 tcagcggcaa caataactcg agcccggtcg ctcccgcctc ggcttcggca aaaggcaaca
      901 gtttggcgtc atcgtcgacc ggcgcgtgat cggagcagac gctgtcgatg gtgccgtcct
      961 tcaggcccat gcgcagagcg tcgcggtcgc gctggctgcg taggggtggg cgcaggtgac
     1021 agttggcgtc gaagaagccg atatcatgct cggtcaggtg caggtggtgg gcgctgatgt
     1081 cgcagctgag tttcacgcct tcctgcttgg cgttacgcac catgtcgatc gcttccgcgg
     1141 tggataggcg gcacaggtga agcttggcgc cggtctcttt cgcaagccgc agcatggtcg
     1201 ccagcgcgag cgtctcggct gcggctggga tgccgcgtaa accgagccgg gcggcgatct
     1261 cgccatcatg ggcaacaccg ttcctggcta ggtagggttc ttccggccgc aaccagaccg
     1321 tgaatccgaa ggtggccgca tattgcatgg cacgccacaa cacctgagtg tcgatgatgg
     1381 cgacatcggc ttgcgagaaa ccgacacaac cggcttcggt caattcgcac atctcggtca
     1441 ggtgctggcc ttctaactga cgggttagcg cgcccagagg atagacatgc gccatattga
     1501 gttgtttggc ccgatgtttg agcatctcca ccaggcccgg ctcatcaagt accggatcgg
     1561 tgtctggcgg gcaggcgagg ctggtgacgc caccggcatt ggctgcgcgc atctcgcttt
     1621 ccagcgtcgc tttgtattcg aagccaggtt cacgtaagcg ggcggatagg tcgaccaatc
     1681 cagggcagac gacgaggccg ctggcgtcga tcacctggtt ggggacgaag ccactcggcg
     1741 cctggccgag gccgacgatc ttgccggcgg cgatatagag gtcgcgctgt tcgtcgatgc
     1801 cgttactggg gtcgatcagc cggccgttct tgatatggat cttcatgctt aattccctgc
     1861 gagtatcgac atgacggcca tgcggacggc gatgccatag gtgacttgcg gcaggatcac
     1921 cgactgacgg ccatcagcga ctgccgagtc gatctcgacg ccgcgattca tggggcccgg
     1981 atgcatgacg atggcatccg gcttcgccag tgccagcttt tgcggcgtaa ggccgtaagt
     2041 cttgaaatac tcctgcgcgc tgggcagtag cgcgccggtc atgcgttcgt tctgcaggcg
     2101 cagcatcatc accacatcta cgccgcgcaa accggcctcc atgtcgtgga acacctgcac
     2161 gccgagtttc tccacatcgt gcggcagcaa ggtcttgggc gcgatgacgc ggacttccgg
     2221 cacacccagc gtggtcaggg cgtggatctc tgagcgggcg acgcgagagt gcagtacgtc
     2281 gccgacgatg gcgacacgca ggttgtgcat ctcgggcttg aagcgacgga tggtaaagac
     2341 gtcgagcaga ccttgagtcg ggtgcgcatg gcgaccatcc ccggcgttga tgacatggat
     2401 gtgcggttcg acatggcgtg cgatcaaatg tgcggcaccg gattgcgcgt ggcgcactac
     2461 gaacatgtcc gcatgcatcg ccgtgagatt gtcgacggtg tcgaggatgg tctctccctt
     2521 ggactgcgaa gaggtgctga cgttgagatt gatgacgtcc gcggacagac gcttggcagc
     2581 gatctcgaac gtggtccggg tacgtgtgct gttctcgaag aagatattgc agaccgtctt
     2641 gccgcgtagc agtgggactt ttttcacttc gcgctcggcg acgccgacga aggactcggc
     2701 ggtgtcgagg atctggttga gtatggtttt gggcaagccc tcgatcgaca gcaggtgctt
     2761 gagcttaccg tcttcggtca actgcgggtt atgcatgcgc agccaccttc gcatctggtc
     2821 cgcagccatc ttgcgatggc gttacgcaca tggcgcaccg catcgttcga tcgttcgcct
     2881 tgctcacttt acggctcctt gcgcgtcatg ccatcccggc aaatgtctgt tcatgctcat
     2941 ccgatacttt cttccaaggt caggtaaaag cggccgtcat catggcgctg caattgcagg
     3001 ttctgttccg gcggcagggt catgtcgtgg gcagtgatct gtggcgtgat cggcagttcg
     3061 cggccgccgc ggttgatcaa tacggcaagc gagatgctcg ctggccgccc gtaatcgaac
     3121 agctcgttca tggcggcgcg gatggtgcgg ccggtgtaga agacgtcgtc gatcagcacg
     3181 atgtgcttgc cttcgacatc gaacgggatc tcggacggct tcggctcgtg atgcagaccg
     3241 cgctggttga aatcgtcgcg gtagaacgag acgtcgagct tgccctgcgc cagatccatg
     3301 cccagcgcag gcaacaggcg ttccatgatc cagacgccac cgctgtggat gcctaccagc
     3361 gcgttatcgg gctggacgat ggatttgagt ttcgcggcca tgttggcgag caggagctcc
     3421 gcgtcgggca aggtcaggtc actgggtttc attggcatag ctgtcgaaat aggattggag
     3481 gatatgctgg gccgctacct ggtccagcat gactttctgc ttgcgacccg aaacgccagc
     3541 ctgcttcaaa gactgactgg cttcggccga ggtcagccgc tcgtcgacca gggtcaccgg
     3601 caggccgaag cggccattga ggcgccgcgc gaacttgcgg cagagctggg tcatcgcatg
     3661 ctcttcgcca tccagatgtg cggggaggcc tacgaccaat ctttgcggtt gccattcctg
     3721 gatcagttcg ccgatgcgac ggaagcgctc atcgttggct tccgtggcga tcgtggtcag
     3781 cggatgcgcg atgccgagca ggtgttcgcc gatggcgacg ccgatgcgac gttcgccgaa
     3841 atcgaaggcc agtacatagc cctgtggctg taacagcgct ggggtgttcg gttgatcggg
     3901 agcggaggcg atggggtcgg gggcggcagt catgcggtca ggcatggccg gcctcctctg
     3961 agagtcgtgc gaaatcgaga ccgagcaggt tcatggcggc atgcagcttg ttctcgctcg
     4021 gcaggtcgaa gatcacggtg tcgctagcct gcaccgacag ccacgcgttc tgcgccattt
     4081 cctgctccag ctggccggcg gaccagcctg catagccgag tgtgatgagg attttgtccg
     4141 gcccctggcc cttggccacg gcttccagga tgtccttgga ggttgtcagc gcagtatcgc
     4201 cattgatggc gatggttgaa tcccaatcgc cggcgggttg atgtagcacg aaaccgcgat
     4261 cgatctggac cgggccgcca aaatgcacgg ggctggcagc gagggggttg tcatcgaggg
     4321 aaaggttgat ctgttcgaac agcgcgtcca gggtcatgtc gatcgggcgg ttgatgacca
     4381 cgcccatggc gccgtcctgg ttgtgttcgc agatgaaagt gaccgatttt gcgaaatacg
     4441 ggtcggtcat cgcgggcatc gcgatgagga agtggccggt aagattcaca ttttccacga
     4501 ggcgtattat ctcattgata tcagtcggtt ggctatctat ttcctaggcc tggccagctt
     4561 cgaggtagcc tgtcacctca agcccgaacc cggtcatgct cggcatcttg cgcggcgtcg
     4621 ccatcagttt catcttgcga acccccaggt caagcaagat ctgcgatccg atcccatagt
     4681 tacgcaggtc ctgcctgggg cggatcggtt cgtccgcgtg caggatgcgc tcgaccagat
     4741 cggcgccatc ctcgttgtga tgcaacagga ccatgacgcc acaagtcgag cgctggatcg
     4801 cctgcatggc cttcagcata ttccacgaat ggctgttgct ggtactgtcc agcaggtcga
     4861 atacggatag cggctcgtgc acccgcacca atacctcctg gtcagcgctc ggcgtaccct
     4921 tgactagtgc caggtgtgtc tccttggcga tgcggtcggc ataagcgatc agctgcatct
     4981 cgccgtacgg cgtctgcacg gtgcgctcgg ctgcgcgctg gatcaggcat tcgtgctggc
     5041 tacgatagtg gatgaggtcg gcgatggtgc cgatcttcaa ttgatgctgg ctggcgaagg
     5101 ccatgaggtc gggcaggcgc gccatgctgc cgtcgtcctt gaggatctcg cagatcactg
     5161 ccgccggctc cagccctgcc agcgaagcca ggtcacaacc cgcctcggtg tgaccggcgc
     5221 ggaccagcac gccgccgttc tcggcgacca gtgggaacac atgaccgggg ctgacaatgt
     5281 ccttgggcga tgcctgtctg gctacggcgg cctgg
//
LOCUS       DATMTW010000291         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_629812, whole genome shotgun sequence.
ACCESSION   DATMTW010000291 DATMTW010000000
VERSION     DATMTW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629812"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..2520)
                     /locus_tag="VN084_05680"
     CDS             complement(34..2520)
                     /locus_tag="VN084_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWT28981.1"
                     /translation="MRILLAWQQLRSQWHSGEVRVLFLALVLAVAATTAVGFFTDRIQ
                     SALIRQGGLLLGADLVVSADHALPPHFAEAAAKADLNSARTIEFPSMAIHGELSRLAE
                     IKAVSERFPLRGTLTIADSVGGPGRAASYIPQPGTAWLEARLASLLALKVGDEVEVGD
                     RKLRVSAILQREPSRGGDMFSIAPRLMMNIADVRSTGLIQFGSRIKYQLLLAADARDI
                     QAYADWAKTRLARGEKVEDVRSARPEMKSALDKAQQFLGLSAMVSVLLAMVAMCLASL
                     PYVQRSLDSFALMRCFGASRADITAILLYQTVFMGVLGSAVGCVVGYAAQAGLAALAG
                     SLFLETLPPAGWMPVIAGFVTGLATMLSVVWPQLSRLRDVPALRILRRDLGERSVLGR
                     FSYLPSIAILCGLVMWHAQSVKLGSVTLATFFGLLLVIGLIAAACGKLLYRYANEGVG
                     GWRLGLANLRRRSRMTFAQVAGFSLGLMALILLALVRSDLLQSWQASLPADAPNRFVI
                     NIQPDQIDGIQEFFVRSGLHGASVHPMVKGRLVEVNGKPLVVARYTDEQARRLAEREF
                     NLSWAADMQLDNTLVAGRWWSLQEHGKPIISLEEGLAKKLGLKLGDTLTYDIAGTRIK
                     LTVTSLRKVEWDTMRANFFAVTPPGVLDRFAASYITSFHLPLGDEITLNQLVKRFPNL
                     TVIDIAELMQQVRGIMQKMTNAVEYVFAFSLLAGLAVLYAALVATREERIREVALLRV
                     LGASRKQLLAALLTEFTAIGIVAALVATLVASGVAWYVSVSLLDIPYQFNLQLALLAL
                     AGAALLVPLAAWLGLRGFLDRPPRTLLHSI"
     gene            complement(2520..3227)
                     /locus_tag="VN084_05685"
     CDS             complement(2520..3227)
                     /locus_tag="VN084_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWT28982.1"
                     /translation="MQTPHAIEAINLDKQILTSEANLVILRKLDLQVARGEKLAIVGS
                     SGSGKSTLLGLLAGMDNPSAGKVLVNAESLSDLDEDGRAALRGRSMGFIFQSFQLLPA
                     LTALENVMLPLQLAGRPQAAETARLALKRVGLQDRLTHYPRQLSGGEQQRVAIARAFA
                     PKPAILLADEPTGNLDQATGQRIIDLLFELNQEAGTTLILVTHDLALASRCERILRLE
                     DGVLHEVALDDSGMGNA"
     gene            3243..>3677
                     /locus_tag="VN084_05690"
     CDS             3243..>3677
                     /locus_tag="VN084_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWT28983.1"
                     /translation="MLFVFVLTLLTSYILPSSALAENERPVKTILVYGDSLSAAYGIP
                     REQGWVSLLEQRIRSQKLPYRVVNASVSGETTSGGLSRFPADLRQHAPDLVILELGAN
                     DGLRGLPVNEMQNNLGKMIQAAKAAKARVLLLGMMMPPNYGHR"
BASE COUNT          743 a         1181 c         1134 g          619 t
ORIGIN      
        1 gatctaccac cgccagcggg ctgtcagcaa ccactagatg ctatgcaaca aggtccgtgg
       61 cgggcggtcg agaaagccgc gcaaacccag ccaggcggcg agaggcacca gcaatgccgc
      121 accggcaaga gccagcaggg caagctgcag gttgaactgg taagggatat ccagcagcga
      181 aacgctgaca taccaggcga caccgctcgc gaccagcgta gcgaccagcg ccgccacgat
      241 cccgatggcc gtgaattcgg tcagcaatgc cgccaatagc tgcttgcgcg aggcaccgag
      301 cacgcgcaac agcgcgacct cgcggatacg ctcctcgcgc gtcgcgacga gcgccgcata
      361 gagcaccgcg agacctgcga gcaaactgaa ggcgaacacg tattcaacgg cgttggtcat
      421 tttctgcatg atgccgcgga cctgctgcat gagttccgcg atatcgatca ccgtcaggtt
      481 gggaaagcgc ttgaccagct ggttaagggt gatctcgtcg cccagcggca ggtgaaagct
      541 ggtgatgtag ctggcggcga aacgatccag cacaccgggc ggagtgacgg caaagaaatt
      601 cgcgcgcatg gtatcccact ccaccttgcg caggctggtg acggtgagct tgatccgcgt
      661 accggcgata tcgtaggtca gcgtatcgcc gagcttgagg cccagcttct tcgcaaggcc
      721 ttcttccagc gagatgatcg gcttgccgtg ctcctgcagc gaccaccagc ggcctgccac
      781 caaggtgtta tcgagttgca tgtccgccgc ccatgacagg ttgaactcgc gctcggccag
      841 ccgccgtgcc tgttcatcgg tgtagcgggc gacgaccaag ggcttgccgt tcacctcgac
      901 cagccgaccc ttgaccatgg ggtgcacact cgcaccatgc agtccggatc gaacgaaaaa
      961 ctcctggata ccgtcgatct ggtcaggctg gatattgatg acaaagcgat tcggcgcatc
     1021 tgccggcaag gacgcctgcc aactttgcaa cagatcgcta cgcaccagcg ccagcaggat
     1081 cagtgccatc aggcccaggc tgaacccggc gacttgcgcg aacgtcatgc gcgagcgccg
     1141 gcgcaggttg gcaagcccca ggcgccagcc gcccacgcct tcattggcat accggtaaag
     1201 cagcttgccg caggcggccg cgatgagacc gatgaccagc aacagcccga agaaggtcgc
     1261 cagcgtgacg ctgccgagct tcacgctttg cgcatgccac atcaccagcc cgcagaggat
     1321 cgcgatactc ggaaggtagc tgaaacggcc gagcacggag cgctcaccca gatcgcgacg
     1381 caggatgcgc aaagccggca catcgcgcaa gcggctcagt tgaggccaga cgacgctgag
     1441 catggtagcg agccccgtga cgaagcctgc gatcacgggc atccaacccg ccggcggcaa
     1501 ggtctcgagg aacaggctgc cagccagcgc ggcgagtccg gcttgtgccg cgtaaccgac
     1561 cacacagccg actgcgctac cgaggacgcc catgaacacg gtctggtaga gcaggatggc
     1621 cgtgatatcc gcccgcgagg cgccaaagca gcgcatgagg gcaaagctgt cgagactgcg
     1681 ctgcacataa ggcaggctgg ccaggcacat ggcgaccatg gcgaggagga cgctgaccat
     1741 cgccgacaaa ccgaggaact gctgcgcctt gtccagcgca ctcttcatct cggggcgtgc
     1801 tgagcgcaca tcctcgactt tctcgccacg cgccagcctg gtcttggccc agtcggcgta
     1861 ggcctggata tctctggcgt ccgcggcgag cagcagctgg tacttgatac ggctaccgaa
     1921 ctggatgagg cccgtggatc gcacatcggc gatattcatc atcagacgtg gcgcgatact
     1981 gaacatatcg ccgccacgcg agggttcgcg ctgaaggatc gcgctgacac gcagtttacg
     2041 atccccgacc tcgacttcgt cgccgacctt gagcgcgagc aggctcgcca gacgcgcctc
     2101 cagccaggcc gttccgggtt gtggaatata gctcgcagcc cgaccggggc cgccgacgct
     2161 atcggcaatg gtgagcgtgc cgcgcaaggg gaagcgttcg ctgacagcct tgatctcggc
     2221 caggcgactc agctcaccgt ggatcgccat actggggaac tcgatggtgc gggcgctgtt
     2281 gagatcggcc ttggccgccg cctcggcgaa atgcgggggc aatgcgtggt ctgcactgac
     2341 gacgaggtca gcgccgagca gcaagccgcc ctgccgaatc agcgccgact ggatacgatc
     2401 ggtgaaaaat cctaccgccg tggtcgcggc gacggccagc accaacgcca ggaataacac
     2461 gcgcacctcg ccagaatgcc attggctgcg cagctgctgc catgcaagca ggatacgcat
     2521 caggcgttcc ccatgccgct atcgtcaagc gcaacttcat gcagtacgcc gtcttccagc
     2581 cgcaggatgc gctcgcagcg cgatgccagc gccagatcat gcgtcaccaa gatcaaggtg
     2641 gtcccggctt cctgattgag ttcgaacagc agatcgatga tgcgctgacc ggtcgcctga
     2701 tcaagattgc cggtaggctc gtccgccagc aggatcgccg gcttcggcgc gaacgcccgg
     2761 gcgatcgcca cccgctgctg ctctccgccg gaaagctgcc gtgggtaatg tgtcaaccgg
     2821 tcctgcaggc cgacccgttt cagggccaag cgcgcggtct cggcagcttg cggacgccct
     2881 gccagctgta gcggcagcat cacgttctcc agcgccgtca gcgctggtag cagctggaag
     2941 gactggaaga tgaaccccat gctgcgacca cgcagggccg cgcggccatc ttcgtccagg
     3001 tcgctcaggc tctctgcatt gaccaggact tttcccgcgc ttgggttgtc cattcctgcc
     3061 agtaagccca ataatgtcga cttgcccgaa ccggagctgc cgacgatcgc gagcttttcg
     3121 cctcgcgcga cctgcagatc gagctttcgc aggatcacaa gattggcttc gctggtaagg
     3181 atctgcttat cgagattgat cgcttctatt gcgtgaggtg tttgcatcca atgaaatccc
     3241 gcatgttgtt cgtgtttgta ctgactttac tgacttccta catcctgccg agttcggccc
     3301 tggcggaaaa cgagcgcccg gtcaaaacca tcctggtcta cggcgacagc ctgtccgcgg
     3361 cttacggcat cccgcgtgag caaggctggg tgagcctgct ggagcagcgt atccggtcgc
     3421 agaagttgcc ttatcgcgtc gtcaacgcta gcgtcagcgg cgaaaccacc agcggcggcc
     3481 tgagccgctt tccggccgat ctacggcaac atgcgccgga cctcgtcatc ctcgaacttg
     3541 gcgccaacga cgggctgcgt gggctgcctg tcaacgagat gcagaacaac ctgggcaaga
     3601 tgatccaggc ggcaaaggcg gcaaaggcac gtgtgctgct actgggcatg atgatgccgc
     3661 ccaactatgg tcatcgc
//
LOCUS       DATMTW010000292         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_227300, whole genome shotgun sequence.
ACCESSION   DATMTW010000292 DATMTW010000000
VERSION     DATMTW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227300"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VN084_05695"
     CDS             <1..577
                     /locus_tag="VN084_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HWT28984.1"
                     /translation="IGEPRAIIDPRLDRWVIMATAATCLAWSLPLPHWLVLPAAVIAA
                     ALHLRRLSGWRSRHVLGRPLLWILHLAYAWIPLGLLLLGLSAIGVVTQSAAFHALTVG
                     AMAGLIVGMITRTALGHTGRALTARLPERCMYVLIMAAALARLAAAMLPAQYHAGALV
                     VSLLCWVSTFVIYVAIYGPYLMRAREDGMEG"
     gene            complement(883..1962)
                     /gene="mutY"
                     /locus_tag="VN084_05700"
     CDS             complement(883..1962)
                     /gene="mutY"
                     /locus_tag="VN084_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWT28985.1"
                     /translation="MTDTAFSARLVDWQKHHGRHDLPWQNTRDPYAIWVSEIMLQQTQ
                     VSAVIGYYQRFLQRFPDIASLAAAEQEEVLQYWSGLGYYSRARNLHTAAQQIMDEHGG
                     VFPQAFADVQALPGIGRSTAAAIVSFAYDSPHPILDGNVKRVFARHFLIEGWPGTPKI
                     EQRLWALAEALLPQQDMVAYTQGLMDLGATLCTRSRPQCTRCPVSQSCQAFAQGRTAE
                     LPTPKPRKSAPQRQTTMLLLLRGNDILLEKRPPSGIWGGLWSLPEAAVDEVPQEIAAR
                     RYGLETEALPAMTPLQHAFTHFRLAITPQPLRVLQSAKADMTNGREPTMTAATIWMPL
                     EDAIGAALPTPVRTLLLRLRQHGLA"
     gene            complement(2058..3083)
                     /gene="arsB"
                     /locus_tag="VN084_05705"
     CDS             complement(2058..3083)
                     /gene="arsB"
                     /locus_tag="VN084_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWT28986.1"
                     /translation="MNLFERYLSLWVGVCIAVGIVLGQALPGLFQAIGQLEFAQVNLP
                     VGLLIWVMIIPMLVKVDFSALHEVRQHVRGIGVTLFVNWLVKPFSMALLAWLFIRQLF
                     APWLQADQLDSYIAGLILLAAAPCTAMVFVWSRLTDGDPLFTLSQVALNDSIMIFAFA
                     PIVGLLLGISAISVPWVTLLISVLLYIVIPVLLAQRWRRALLRRGQVAFDTAMARIGP
                     WSIAALLMTLVLLFAFQGQAILRQPLVIALLAIPILIQVLFNAGLAYWLNRKLGERHD
                     VAGPSCLIGASNFFELAVAAAISLFGFNSGAALATVVGVLIEVPLMLLVVHVVNRSKA
                     WYQHGSP"
     gene            complement(3089..3592)
                     /locus_tag="VN084_05710"
     CDS             complement(3089..3592)
                     /locus_tag="VN084_05710"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWT28987.1"
                     /translation="MQDRTFNVLILCTGNSARSIMAEALFNILGNGRFKAYSAGSHHT
                     GKVNPFAIEQVQAIGYPVDGLRSKNWDEFATPDAPQMDFVITVCDNAAGEVCPVWPGQ
                     PLSAHWGFEDPAAVEGTDDEKRAAFRKIFHQIRNRVGIFASLPLANLDRIAIKREMDQ
                     IGNRQPE"
     gene            complement(3678..>3985)
                     /locus_tag="VN084_05715"
     CDS             complement(3678..>3985)
                     /locus_tag="VN084_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWT28988.1"
                     /translation="LAALAQESRLSVFRLLVEAGPQGMPAGKISETTGIPPSSLSFHL
                     KELAHAGLLTSRNEGRFVIYAASFTQMNALLAYLTENCCGGNPCTPVSSPACDCEAG"
BASE COUNT          755 a         1202 c         1293 g          735 t
ORIGIN      
        1 tatcggcgag ccgcgcgcaa tcatcgatcc gcggctcgat cgctgggtca tcatggcgac
       61 ggcggcgaca tgcctggcgt ggagcctgcc actgccgcac tggctggtac tgccggcggc
      121 ggtcatcgca gccgcactgc atctgcggcg gctcagtggc tggcgttcac ggcatgtgct
      181 gggtcgaccg ctgctctgga tcctgcatct cgcatatgcc tggatcccgc tcggactcct
      241 gctacttggg ctgtcagcca ttggggtggt gacgcagagc gcggcattcc atgcgctgac
      301 ggtcggcgcg atggcgggcc tcatcgtcgg catgatcacg cgcaccgcac tcggccatac
      361 cgggcgggcg ctcactgcgc gcttgcccga gcgttgcatg tatgtgctga tcatggcggc
      421 agccctcgcc aggctggctg ctgccatgtt gccggcccag tatcatgcgg gcgcgctggt
      481 ggtcagcctg ctgtgctggg tcagcacatt cgtcatctac gtggcgatct atggccctta
      541 cctgatgcgg gctcgtgagg acgggatgga gggttgatcc ccggacgcga gccataaaaa
      601 aacgccagcg agacgctggc gtttctggat cgatcctgct gaacgtgcgc gcgctcaatc
      661 cacctttttc gagatcgcgt aaagaatgac gccgatgacg atcagaatgc cgatcagggt
      721 atagatacca gtagaacctt gaaattccat gctgcctccc gcgttgtcga tgatcgaata
      781 gttgtggttg aatcgtcgac cgatcgcgct gatcggccgt ttccggacag tgcatatgca
      841 aaccgtgccg aggccattat ggcccaaaat cgcggccggg gtctaggcca ggccatgctg
      901 ccgcaagcgc agcagcagtg tgcgtacagg ggtcggcagt gcggcgccga tcgcgtcttc
      961 gagcggcatc cagatggtgg ctgccgtcat agttggctca cggccgtttg tcatgtcagc
     1021 cttggcgctc tgcagcacac gcagcggctg aggtgtgatg gcgagcctga aatgcgtgaa
     1081 tgcatgctgc aacggcgtca tggcaggcaa cgcttctgtc tccaggccat agcgccgcgc
     1141 cgcgatctcc tgtggcactt catccaccgc agcctccggc aagctccaca gcccacccca
     1201 gatgccgctg ggtgggcgtt tttcaagcag gatatcgttc ccgcgcaaca gcaacagcat
     1261 ggtggtttgt cgctgtggcg cggacttgcg cggtttcggc gtcggcagtt cggcggtccg
     1321 gccttgtgcg aatgcctggc aactctgcga gaccggacaa cgtgtgcact gcggccggct
     1381 gcgcgtgcac agcgtggcac cgagatccat cagtccctgc gtataagcga ccatgtcctg
     1441 ttgcggcaac aaggcttcag ccagcgccca tagtcgctgt tcgatcttgg gcgtgcccgg
     1501 ccagccttcg atgaggaaat ggcgcgcgaa cacccgtttg acattgccgt ctaggatggg
     1561 atgtgggctg tcataagcga aggagacgat cgccgccgcc gtcgagcgac cgatgcccgg
     1621 caaggcctgc acgtcagcaa aggcctgggg aaacacgccg ccgtgctcgt ccatgatctg
     1681 ttgtgcggcg gtgtgcagat tgcgtgcccg cgagtaataa ccgagcccgc tccagtactg
     1741 caggacttcc tcctgctcgg ctgcggcgag gctggcgata tccgggaagc gctgcaagaa
     1801 gcgctggtag tagccgatga ccgccgacac ctgcgtctgc tgcagcatga tctccgacac
     1861 ccagatggca tacgggtcgc gcgtgttctg ccaaggcagg tcgtgccggc catgatgctt
     1921 ttgccaatct accagccggg cggaaaatgc ggtatcggtc atgcggttca gcttgagttc
     1981 ttgcctgggt tgagtatcgg agggtgcgcg tctgcccgca ttatcgcatc ttggcagacg
     2041 cagcaggcgt tcagcgttca gggcgatccg tgttgatacc aggctttgct gcgattgacg
     2101 acatgcacta ccagcaacat cagcggcact tcgatcagca ccccgacgac ggtcgccagg
     2161 gcagcgcccg agttgaagcc gaacagactg atggcggcgg ccaccgccag ctcgaagaaa
     2221 ttgctggcgc cgatcaggca cgatgggccg gcgacgtcgt gcctttcgcc cagcttgcgg
     2281 ttgagccagt aggcgagacc cgcattgaac agcacctgga tcaggatggg gatggcgagc
     2341 aaggcgatga ccagcggctg ccgcaagatg gcctgaccct ggaaagcgaa cagcagcacc
     2401 agcgtcatga gcaacgctgc gatcgaccag gggccgatgc gtgccatggc ggtatcgaag
     2461 gcgacctggc cacgccgcag cagcgcacgc cgccagcgct gggccagcaa caccgggatc
     2521 acgatgtata gcaggacgga gatcagcagc gtcacccagg gcacactgat cgcagagatg
     2581 ccgagcagaa ggccgacgat gggcgcaaag gcgaagatca tgatgctgtc gttgagggcg
     2641 acctgcgata gcgtgaacag tggatcgcca tcggtcagcc ggctccagac aaataccatg
     2701 gcggtgcagg gtgccgccgc cagcaggatg agcccggcga tataactatc gagctggtcg
     2761 gcctgcagcc agggcgcgaa cagttgccgg ataaagagcc aggccagcaa ggccatgctg
     2821 aagggtttca ccagccagtt gacgaacagg gtgacgccga tgccgcgaac gtgctggcgc
     2881 acctcatgca gcgcgctgaa atcgaccttg accagcatgg ggatgatcat gacccagatc
     2941 agcaggccca ccggcaggtt gacctgcgcg aattcaagct ggccgatcgc ctggaacaga
     3001 cccggcaacg cctgaccgag tacgatgccg acggcgatac agacccccac ccaaagcgaa
     3061 agatagcgct cgaacagatt catgcgttct actccggttg gcggttgccg atctggtcca
     3121 tctcgcgctt gatcgcgatg cgatcaagat tggccagggg gaggctggcg aatatgccga
     3181 cgcggttgcg gatctggtgg aagatcttgc ggaaggcggc acgcttctcg tcatccgtgc
     3241 cctcgaccgc ggccgggtcc tcgaaacccc agtgagcgct cagcggctga cccggccaga
     3301 ccgggcacac ttcgccggcg gcgttgtcgc aaacggtgat gacgaaatcc atctgcggcg
     3361 catccggcgt ggcgaattca tcccagttct tgctgcgcag gccatcgacc ggatagccga
     3421 tcgcctgcac ctgctcgatg gcaaacggat tgactttgcc cgtgtggtgg ctgcccgcgc
     3481 tataggcctt gaatcgacca ttaccgagga tgttgaacaa ggcttcggcc atgatgctgc
     3541 gggcagaatt gccggtgcac agaatgagta cgttgaaagt cctgtcttgc atggggtttc
     3601 cagtcaaagg tgatgatgtg aacagctatt ctggtgcgcg cgtggcgtct cgcacgccgt
     3661 cagtcgattg cctgcgctca acctgcctca cagtcgcacg ctggcgaact gacgggcgtg
     3721 caggggttgc cgccacagca gttctcagtg aggtacgcca gcaacgcgtt catctgcgtg
     3781 aaactggcgg catagatgac gaatcggccc tcgttgcgcg acgtgagcag ccctgcatgc
     3841 gccagctcct tcagatggaa cgacagcgac gatggcggga tgccggtggt ctcgctgatc
     3901 ttgcctgcag gcatcccctg cgggccggcc tcgaccaaca gccggaagac gctgagacgg
     3961 gattcctgcg caagcgcggc aagtg
//
LOCUS       DATMTW010000293         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_529026, whole genome shotgun sequence.
ACCESSION   DATMTW010000293 DATMTW010000000
VERSION     DATMTW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529026"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /gene="rimI"
                     /locus_tag="VN084_05720"
     CDS             complement(<1..424)
                     /gene="rimI"
                     /locus_tag="VN084_05720"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWT28989.1"
                     /translation="MNALLKDALCFRPMQMADLDTVMAIEPTIYPYPWTRGNFSDSLN
                     AGYSAWVYEEHGHIIGYAVVMLVLDEAHLLNLSIAHTHQGRGLGRALLEYMMQISRGH
                     GAANIFLEVRPSNTPAIRLYEQVGFSEMAVRRGYYPAAH"
     gene            complement(481..1176)
                     /gene="tsaB"
                     /locus_tag="VN084_05725"
     CDS             complement(481..1176)
                     /gene="tsaB"
                     /locus_tag="VN084_05725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWT28990.1"
                     /translation="MKLLALDTSTEYLTLALSLDGEIRTRELHAGQTHSQRILPLLRE
                     LLDETGMALTQLDGIAFGAGPGSFTGLRIGCGVAQGLAFGANLPVVGVCTLQALAEAS
                     GSDKVIACLDARMGEVYHAVYVRQAGVWQEVLAPGLYKPEAVPAVEGEGWIGAGSGWK
                     TYAEILGDIHAGHLTKTFPDAYPHAAAIAQLAVPAFAAGQGKPAAEAAPIYVRNKVAL
                     KTSEREAGMKLGA"
     gene            complement(1173..3116)
                     /locus_tag="VN084_05730"
     CDS             complement(1173..3116)
                     /locus_tag="VN084_05730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWT28991.1"
                     /translation="MSDLDLVFAGDGPLAETIPGYRMRQQQLEMAQAIEDAIKHGKQL
                     VAEAGTGTGKTFAYLIPALLSGGKVIVSTGTKTLQDQLFNRDLPAVRDALKVPVTVSM
                     LKGRANYVCHFHLERAMHDGRFLSREDANYVHKIKAFAEHTDTGDKSELTDVPENATI
                     WPAVTSTRDNCMGQECNFYKDCFVMEARKRALAADVVVVNHHLFFADVMLRDEGVAEL
                     LPSANTVIFDEAHQLPEVAGLFFGEDVSTSQLIELARDTHAEAVTTAKDCLALPEATA
                     ALEKAVRDFRLIFAYEGSRMPVQKALALKGFETAYATMQEKLKALSAILETQAERDPA
                     LENCWQRSLALEVQLQRWSRAENENLVRWVEVFTQSVQLHATPLSVAEGFGKQLNAQP
                     RSWIFTSATLAVKSDFSHYLAQMGLEQADTGYWSSPFDYGQQALLYVPKEMPEPNSPG
                     YTASVAAAALPVIQASQGRAFVLCTSLRAMREVHALLKDAFSSNGMEYPLLLQGDSAR
                     TELLERFRKLGNAVLVGSQSFWEGVDVRGEALSAVIIDKLPFAPPDDPVLSARIDKMN
                     AEGRNAFMEYQLPYAVITLKQGAGRLIRDETDTGVLMICDPRLISKPYGRRIWQSLPP
                     FRRTRELTDVVEFFDNMAAIKAE"
BASE COUNT          609 a          993 c          997 g          642 t
ORIGIN      
        1 catgcgctgc cgggtaatac ccgcgacgca cggccatctc actgaagccg acctgttcat
       61 agagccggat cgcgggtgtg ttggaaggcc gcacttcgag aaaaatattg gctgcgccgt
      121 gtccgcgcga gatctgcatc atgtattcca gcagcgcgcg gccgagaccg cggccctgat
      181 gggtatgggc gatactgaga ttgagcaggt gcgcttcgtc cagcaccagc atcaccacag
      241 cgtagccgat gatatgacca tgctcttcat agacccaggc gctgtaaccg gcattcaacg
      301 aatcgctgaa attgccgcgc gtccacggat aaggatagat ggtcggctcg atcgccatca
      361 cggtatcgag atcggccatt tgcatgggcc gaaaacataa ggcatctttc aacaaggcgt
      421 tcatcgctgg ggcagttttc ctggattggt ttgccgggat tcgtttcctg aaagcgtctt
      481 ttaagcaccg agcttcatgc cggcttcgcg ctcgctggtc ttcagcgcga ccttgttacg
      541 cacatagatg ggcgccgctt cggcagcagg tttgccctga cccgcggcaa acgccggtac
      601 cgccaattgc gcgatggccg ccgcatgtgg ataggcgtcg gggaacgtct tggtgagatg
      661 gccggcatgg atatcgccga ggatctccgc ataagttttc cagccgctgc cggcaccgat
      721 ccagccttcg ccttcaacgg caggcacggc ttcaggctta tacaaaccgg gagccagcac
      781 ttcctgccag acgccagctt gtcgcacata gaccgcgtga tacacctcac ccatgcgcgc
      841 gtcgaggcag gcgatgacct tgtccgagcc gctcgcttcc gccaacgctt gcagcgtgca
      901 gacgccgacc accggcaggt tggcgccgaa cgccaggccc tgggccacgc cgcagccgat
      961 gcgcaagccg gtgaacgaac cggggccggc gccgaaggcg atgccgtcta gttgcgtgag
     1021 cgccatgcca gtctcgtcga gcagttcgcg cagcaagggc aggatccgct gtgagtgggt
     1081 ctggcccgca tgcagttcgc gcgtgcgtat ctcgccatcg agcgagagcg ccaatgtcag
     1141 gtattcggtg gaggtatcga gtgcaagtag cttcattcgg ctttgatcgc ggccatgttg
     1201 tcgaagaatt ctactacgtc cgtcagttcg cgcgtgcgcc tgaacggcgg caggctctgc
     1261 cagatacgcc tgccgtaggg tttgctgatg agtcgcgggt cgcagatcat gagcacgccg
     1321 gtatcggtct cgtcgcggat cagcctgccc gcaccctgtt tcagcgtgat gacggcataa
     1381 ggcagctggt attccatgaa ggcgttacgg ccctcggcgt tcatcttgtc gatgcgggcc
     1441 gacaacaccg ggtcgtccgg tggcgcgaac ggcagcttgt cgatgatgac ggcgctcaag
     1501 gcttcaccac ggacgtccac gccttcccag aagctttgcg accccaccag cacggcatta
     1561 cccagtttgc ggaagcgttc aagcagctcg gtgcgcgcgc tatcgccctg cagcaatagc
     1621 gggtactcca tgccgttgct ggagaacgca tccttgagca gggcgtgcac ttctcgcatg
     1681 gcacgcaggc tggtacacag cacgaatgcc cggccctggc tcgcctggat caccggtaac
     1741 gctgcggcgg cgaccgaggc cgtatagccg gggctgttgg gttccggcat ctccttcggc
     1801 acatacagca gcgcctgctg gccgtaatcg aaggggctgc tccagtaacc ggtatcggcc
     1861 tgttccaggc ccatctgcgc caggtaatgg ctgaaatcgc tcttcaccgc gagcgtcgcc
     1921 gaggtgaata tccaggaacg cggctgggcg ttgagctgct tgccgaaacc ttccgctaca
     1981 gacaacgggg tggcatgtag ctgcaccgat tgcgtgaaga cttccaccca gcgcacgagg
     2041 ttctcgttct cggcacgcga ccagcgctgc agctgcacct ccagcgccag gctgcgctgc
     2101 cagcaattct caagcgccgg gtcgcgctcg gcctgggttt cgaggatcgc gctcaacgcc
     2161 ttgagctttt cctgcatcgt cgcataagcc gtttcgaacc ccttgagcgc cagcgccttt
     2221 tgcaccggca tgcgtgagcc ttcgtaggcg aagatcagcc ggaaatcacg caccgccttc
     2281 tccagcgcgg cggtcgcttc cggcaaggcc aggcaatcct tggcggtagt caccgcctcg
     2341 gcatgggtat cccgcgccag ctctatcaat tggctggtcg agacatcctc accgaagaaa
     2401 aggccggcca cttccggcaa ctgatgagcc tcgtcgaaga tgacggtgtt ggcacttggc
     2461 aacagctcgg ccacgccttc gtcgcgcagc atgacatcgg cgaagaacag gtgatggttg
     2521 accaccacga catccgctgc cagcgcgcgc ttgcgggcct ccatcacgaa acaatccttg
     2581 tagaaattgc actcctggcc catgcagtta tcgcgggtgg aagtcacggc cggccagatc
     2641 gtcgcgtttt cgggcacatc ggtcagttcg ctcttgtcgc ccgtgtccgt gtgctcggcg
     2701 aacgccttga tcttgtgcac gtagttggcg tcttcgcgag acaggaaccg cccatcgtgc
     2761 atggcgcgct cgagatggaa atggcagacg tagttggcgc gccccttgag catggagacc
     2821 gtgaccggta ccttgagcgc gtcgcgcacg gccggcaagt cgcggttgaa caattggtcc
     2881 tgcagggtct tggtgccggt agagacgatg actttgccgc ccgacagcaa ggccggaatc
     2941 aggtaggcga aggtcttgcc ggtgccggtc ccagcctcgg cgaccagctg cttgccgtgc
     3001 ttgatggcat cctcgatggc ctgcgccatc tccagctgct gctggcgcat gcggtagccg
     3061 ggaatggtct ccgcaagggg gccatcaccg gcaaaaacaa ggtctaggtc actcacggga
     3121 atccggggat caagcataga tgaatggaca agtgtccatt ttaactggcc tgtcgaaaca
     3181 ggaatgaatt atgttgtgat gcgggtacgt ttttacctgt cagaagctac aagcagggat
     3241 a
//
LOCUS       DATMTW010000294         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_529139, whole genome shotgun sequence.
ACCESSION   DATMTW010000294 DATMTW010000000
VERSION     DATMTW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529139"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VN084_05735"
     CDS             <1..666
                     /locus_tag="VN084_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HWT28992.1"
                     /translation="EDMNGSAVALGILLAATQAELPVKLDCCLAIAENHIGPKAYKQN
                     DVVTALNGKTIEIVHTDAEGRMVLADTLTLASRYKPELLIDFATLTGSMVTAVGDRYS
                     GILGNRPELLCKAVGAGVVAGERVNAFPFDEDYDEDLDSDIADIKQCTLDSEADHVLA
                     ARFLSKFIEHDVPWLHVDLSACRRKGGLGAVDTDVTGFGVGFALTFIAQWMAERTPSK
                     GGK"
     gene            666..1328
                     /locus_tag="VN084_05740"
     CDS             666..1328
                     /locus_tag="VN084_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWT28993.1"
                     /translation="MQTTETLAWLAQFPALMDIEPEARALLARHARIVEAPVGTIGYS
                     EGMPCKGYVLRLAGQSRVYKISSSGREILLYRVAAGETCVLTTTCLLGRSDYPASTVV
                     EEPIRDVVVPAAIFHQLMIESPVFRRFVMENYGALISDLIVLLDEVAFHSLDTRLAKL
                     LLDESGPQIHRTHQQLADELGTAREVVSRQLKRFEQKGWVSLGRGQIEVLDRDALERA
                     AR"
     gene            complement(1381..>1816)
                     /locus_tag="VN084_05745"
     CDS             complement(1381..>1816)
                     /locus_tag="VN084_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWT28994.1"
                     /translation="IGTLGNVDMRDANVDKGRFGLDIGNQPMGAMRFDAVGGKFAHTN
                     FEGADINRANLQFADFRGANLRHTDLFRADLSKADLTGADITGANLKEAVLDGTVMTE
                     VKGLAAIEGYDIKKGKCIDCKVTADAAKTAGKDAAACVMDKK"
BASE COUNT          346 a          525 c          581 g          364 t
ORIGIN      
        1 gaagacatga atggttcggc ggtggcgctg ggcatcctgc tggcggctac ccaagcggaa
       61 ttgccggtga agcttgactg ttgtttggcc atcgccgaga accatatcgg ccccaaagcc
      121 tacaagcaga atgacgtggt caccgcgctc aacggcaaga ccatcgagat cgtgcatacc
      181 gacgccgaag gccgcatggt actggccgac accctgacgt tggcctcacg ctacaagccg
      241 gaactgctga tcgatttcgc caccctgacc ggcagcatgg tcacggcagt gggagaccgt
      301 tacagcggga tactcggtaa ccgaccggag ctcttgtgca aggcggtggg agccggcgtg
      361 gttgccggcg aacgcgtcaa cgcctttccg ttcgacgaag actatgacga ggacctggac
      421 agcgacatcg ccgacatcaa gcaatgcacg ctggattcgg aagccgacca tgtgctggct
      481 gcacgattcc tgtcgaagtt catcgagcac gatgtgccct ggctgcatgt cgatctttcc
      541 gcgtgtcgac gcaaaggggg gctgggtgcc gtcgacaccg atgtcaccgg gttcggtgtc
      601 ggattcgcgc tgacgttcat cgcgcaatgg atggctgagc gtaccccatc aaaagggggc
      661 aagtgatgca gacgactgaa acgctggcct ggctggcgca attcccggcc ttgatggaca
      721 tagagccgga ggccagggcg ttgctggccc ggcatgccag gatcgtcgaa gcaccggtcg
      781 gcactatcgg ctattcggaa ggcatgccgt gcaagggcta tgtactgcgg ctggccggcc
      841 agtcccgggt gtacaagata tccagcagtg gccgcgagat cctgctgtac cgcgtggcgg
      901 caggcgagac ctgcgtgctg accacgacct gcctgctcgg tcgcagcgat tacccggcgt
      961 cgacggtagt cgaggagccg atccgtgacg tcgtcgtgcc tgcggcgatc ttccaccagc
     1021 tgatgatcga atcaccggtg ttccggcgct tcgtgatgga gaattatggc gccttgatca
     1081 gtgacctcat tgtgttgctg gatgaagtcg cttttcatag cctggacacc cggcttgcca
     1141 agttgctgct cgatgaatcc ggaccgcaga tccatcgcac tcaccagcaa ctggcagacg
     1201 aactcgggac agcacgcgag gtggtcagtc gacagttgaa gcgtttcgaa cagaaaggct
     1261 gggtcagcct ggggcggggg cagatcgaag tgttggatcg cgatgcgctg gagcgcgccg
     1321 cacgctgaga tacattaaat ggccaggcgc ggtatcgcgc ccggccagct tggcattgac
     1381 ctacttcttg tccatgacgc aggcggcagc atccttgcct gcggtctttg cagcatccgc
     1441 agtgacttta caatcgatgc acttgccttt cttgatgtcg tagccttcga tcgccgccag
     1501 gcctttgact tctgtcatga ccgtgccgtc gagcacggct tccttgaggt tggcgccggt
     1561 gatatcggct cctgtcaggt cggccttgct aaggtcggcg cggaacaagt cggtgtgccg
     1621 caggttggca ccacggaagt cggcgaactg cagattggcg cggttgatgt cggcgccttc
     1681 gaaattggtg tgagcgaact tgccgccaac ggcatcgaat cgcatggcgc ccatcggctg
     1741 gttgccgata tcgaggccga agcgaccctt atcgacattg gcgtcgcgca tgtccacatt
     1801 gcccagagtg ccgatc
//
LOCUS       DATMTW010000295         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_495653, whole genome shotgun sequence.
ACCESSION   DATMTW010000295 DATMTW010000000
VERSION     DATMTW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495653"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VN084_05750"
     CDS             <1..1491
                     /locus_tag="VN084_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWT28995.1"
                     /translation="LLTATVARSPVFGGKVKSFDDSATRKVRGVRNVVQVPTGVAVIA
                     DHYWAAKQGRDALKVEWDLGENAQLDTTTMLAEYRKLAGEKGLPAAKVGDSQAAMAKA
                     VKTVEAEYVAPYLAHSPMEPLNCAVRINDDGCEVWTGTQMQGTDQQAVAKITGFKPEQ
                     VKIHTMFLGGGFGRRANPAADFVSEAAHVAKAAGAPVKTVWSREDDVKGGYYRPMFVH
                     KARIGLAADGKPVAWEQSIVGQSIALGTPFEGFMIKNGVDSTSVEGVADSPYLKEIQN
                     HNIELHTVKATIPVLWWRSVGHSHSGFVMESLVDEAAHAAGRDPLIYRRELLKDHPRH
                     LAALNLAAEKADWGKPLPKGVARGIAVHESFGSFVAQVAEVSVEDGTVKVHRMVCAID
                     CGLAVNPESLRAQMESSIAFGLGAALQSEITFKGGQVQQSNFHDYLVLRMNQMPKVEV
                     HIVPSQEKMGGVGEPALPPVAPAVTNAIFALTGKRIRSLPIGSQLA"
     gene            1497..>1880
                     /locus_tag="VN084_05755"
     CDS             1497..>1880
                     /locus_tag="VN084_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWT28996.1"
                     /translation="MQALDHEVLQRATEWLAQGRGVHLFTVVQTWGSAPRLPGAVLAI
                     RDDGHLVGSVSGGCIEDDLADKARHGQLPETASLIEYGVSRDEAQRFGIPCGGRLLIF
                     AEPLRDSVALIQIMHGMQQRKLIKRS"
BASE COUNT          359 a          576 c          606 g          339 t
ORIGIN      
        1 ctgctgacgg cgaccgtcgc ccgttcgccg gtgtttggcg gcaaggtcaa atcgtttgac
       61 gatagcgcca cgcgcaaggt gcgcggcgta cgcaacgtgg tgcaagtgcc gacaggcgtg
      121 gccgtcatcg ccgatcacta ctgggctgcc aagcagggcc gtgacgcgct caaggtggaa
      181 tgggacctgg gcgagaacgc ccagctcgat accacgacca tgctcgccga atacaggaag
      241 ctggccggcg agaagggtct gccggcggcc aaggtcggtg atagtcaggc agcgatggcg
      301 aaggccgtga agacggtcga ggccgaatac gtcgcgccgt atctcgccca ttcgccgatg
      361 gagccactca attgcgccgt gcgcatcaac gatgacggtt gtgaagtctg gaccggcaca
      421 cagatgcaag gtaccgatca gcaagccgtg gccaagatca cgggcttcaa gccagagcag
      481 gtcaagatcc acaccatgtt cctcggtggc ggtttcggac gtcgcgccaa tccggctgcg
      541 gatttcgtct ccgaagctgc gcacgtcgcc aaggcggccg gcgcaccggt aaagacggtc
      601 tggagccggg aggatgacgt gaagggtggc tactatcgtc cgatgtttgt gcacaaggcg
      661 cgcatcggcc tcgccgccga cggcaagccg gttgcctggg agcaatccat cgttggccaa
      721 tccatcgcgc tcggtacgcc attcgagggg ttcatgatca agaacggcgt cgattcgacc
      781 tctgtcgaag gcgttgccga ttcgccctat cttaaggaga tccagaacca caacatcgag
      841 ttgcacacgg tcaaggcgac catcccagtg ttgtggtggc gatcagtcgg tcatagccat
      901 agcggtttcg tcatggaaag cctggtcgat gaggcagcac atgccgccgg gcgtgacccg
      961 ctgatctatc gtcgcgaatt gctgaaagac catccgcggc atctggctgc actcaacctg
     1021 gcggcagaga aggccgattg gggtaagcct ttgcccaaag gcgtcgcgcg cggcatcgcc
     1081 gtgcatgaat cgttcggcag tttcgtcgcg caggtggccg aagtgtcggt ggaagacggc
     1141 actgtcaaag tacaccgcat ggtctgcgcc atcgattgcg gtctcgccgt gaatcctgaa
     1201 tcgctcaggg cgcagatgga gtcatccatc gccttcggcc tcggcgctgc cctgcagagc
     1261 gagatcacgt tcaagggagg ccaggtgcag caatccaact tccatgatta cctggtcttg
     1321 cgcatgaacc agatgccgaa ggtcgaggta cacatcgtgc cgagtcagga aaagatgggc
     1381 ggcgttggcg aacccgcctt gccgccagtg gcgccggctg tcaccaatgc catcttcgcg
     1441 ctcaccggca agcgtatccg cagcttgcct atcggcagcc agctggctta ggcctcatgc
     1501 aggcactcga tcacgaggtc ttgcagcgcg ccaccgaatg gctggcgcag ggccgcggcg
     1561 tccacctgtt caccgtggtg cagacctggg gttcggcccc gcgcctgccc ggtgcggtgc
     1621 tggccattcg cgacgacggc catctggtcg gttcggtgtc cggcggctgc atcgaggacg
     1681 acctggccga caaggcgcgc catggccagt tgcccgagac cgcctcgctc atcgagtacg
     1741 gcgtcagccg cgatgaagcg caacgcttcg gcatcccctg cggcggtcgc ctgctgatct
     1801 tcgccgaacc cctgcgtgac agtgtggcgc tcattcagat catgcatggc atgcaacaac
     1861 gcaagctgat caagcgttct
//
LOCUS       DATMTW010000296         5181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_495880, whole genome shotgun sequence.
ACCESSION   DATMTW010000296 DATMTW010000000
VERSION     DATMTW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495880"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VN084_05760"
     CDS             <1..935
                     /locus_tag="VN084_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWT28997.1"
                     /translation="WQKLSRNGIAGVRRHYTWDGHVAKYLKSVGQVLHQSRKRMRREM
                     ALSLRLKRTPIPLAQKALISDIDNTLLGDERGLRELITLLRKHSDQMAFGIATGRSID
                     SAVKILKKWQVPIPDVLITSVGSEIHYWPSQRLDLGWSHHISYQWRRDALENFFSAIP
                     GLTLQAAENQREFKLSYNVDPEQMPPIKELYKQLRQLRLKAKLIYSHGSFLDILPERA
                     SKGQAIRYLAYKWGLSLKQFVVAGDSGNDEAMLVGDTLGVVVGNHSPELECVRGLDQV
                     YFAEAHYAAGIVEGLRHYRFIEEHSEPAQEETAA"
     gene            932..3313
                     /locus_tag="VN084_05765"
     CDS             932..3313
                     /locus_tag="VN084_05765"
                     /EC_number="2.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016157 - sucrose synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005985 - sucrose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose synthase"
                     /protein_id="HWT28998.1"
                     /translation="MIQTLTEFTEHHRDAIYTLLRRYFALDRQLLLQSDMRGELDALK
                     AQLKLDEAALKPLVDFVTQLHEGVMISPWAYFTVRPAVGQFDYLRIHQDNLIPERVSP
                     AEFLMFKEQLAHHARPASSLEIDFGPFSREFPKLRETRSIGQGLLFLNRQLSSGMFTH
                     PQRGAEKLLHFLGVHTIEGQQMMFLSTPRDTATAPSVLRSAIDFLETQDETTPWTAFA
                     DRFAKLGFAPGWGNNAGRVAETMNLLMDILEAPSPAALESLLARIPMISRILIVSPHG
                     YFGQANVLGLPDTGGQVVYILDQVRALEKEMRERLLEQGVEVMPKILIGTRLIPDSAG
                     TTCSQRLEKVNGCENTWILRVPMQRANGEIVQQWISRFEIWPYLEDYSYQLDREALAE
                     FSGRPDLIIGNYSDGNLVASLLSQRLNVTQCNIAHALEKTKYLHSDLFWQDSDDKYHF
                     SCQYTADLMAMNCADFIITSTYQEIAGTEHIEGQYESYRTFSMPGLYRVINGVDIFDP
                     KFNIVSPGANADVYFPYSAEERRLSSLWPEIENMIYGGAMPSARGVLKDKKKPLIFTM
                     ARLDRIKNLAGLVEWYGRSEDLRKRANLLVVGGYVDASHSSDHEEQAQIRQMHALMDQ
                     YKLDGEMRWLGMRLDKNQSGELYRYVADRHSVFVQPALFEAFGLTIIEAMASGLPTFA
                     TCYGGPREIIQHNRSGFHINPNEGEAAAALISDFFDRCKANPDEWQRISDGALARVAS
                     HYTWEKYAQRVMTLSRIYGFWKFVSGLERQETARYIDMFYHLQFRPLAERIPR"
     gene            3382..4257
                     /locus_tag="VN084_05770"
     CDS             3382..4257
                     /locus_tag="VN084_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWT28999.1"
                     /translation="MHLSAPAFHILIILVLAAIAFRLSRRLIRVLKEFMLQRAEDNPE
                     EFKRIETLGRVFRYITSVVITIVAGMLILSELGISIAPILAAAGVLGLAIGFGAQSLV
                     KDYFSGFFLLLENQIRQGDVVNVADKGGFVEEMTLRYVKMRDYEGNVHFIPNGIITTV
                     TNMSRQFAYAVIDIGVSYREDVDEVIAVMEQVGKELREDEALAPKILANIEIAGVDQF
                     ADSSVIIKCRFMVQALEQWTVKRAYLKRIKEVFDERGIEIPFPHLTIYPGVLKDGSAP
                     ALRIGAAQEAMQTPS"
     gene            4323..4922
                     /locus_tag="VN084_05775"
     CDS             4323..4922
                     /locus_tag="VN084_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HWT29000.1"
                     /translation="MNPIRMLRYVTHRSDTWHTLAAEQPSVMKMLWSYIVPMSLIPPV
                     MLNIVARNHPRIFMDILPGDRPLIVGLILFLALVVGVLVMAWISKFLAEMVDLKPSYR
                     DALLVIAVAATPFWVASICYLVPNLALNLVLHGVAGVFSVALVYQGILTIFHLHRRGA
                     AAMLTTAIGCTGAIGFGVLLVGMLMTWGVVQDLQFAVKA"
BASE COUNT         1049 a         1658 c         1486 g          988 t
ORIGIN      
        1 aatggcagaa actctcccgc aacggcatcg ccggtgtccg gcggcattac acctgggatg
       61 gccatgtcgc caaatacctg aaatccgtcg gccaggtgtt gcaccagtcg cgcaagcgca
      121 tgcggcgcga gatggcgctg agcctgcgcc tcaagcgcac gccgatcccg ctcgcgcaga
      181 aagccctgat cagcgatatc gacaacacct tgctgggtga cgagcgcggc ttgcgcgagc
      241 tcatcacgct gttgcgcaag cacagcgacc agatggcgtt cggtatcgcc accggccgca
      301 gcatcgatag cgcggtgaag atcctgaaga aatggcaggt gccgatcccc gatgtgctca
      361 tcacctcggt cggcagcgag atccattact ggccctcgca gcgactcgat ctgggctgga
      421 gccaccacat cagctaccag tggcgtcgcg acgcgctgga aaatttcttc agcgcgatac
      481 cgggcctgac cttgcaggcc gccgagaacc agcgtgaatt caaactcagt tacaacgtcg
      541 accccgagca gatgccgccg atcaaggagc tgtacaagca gctgcgtcag ttaaggctca
      601 aggccaagct catctactcg cacggcagtt tcctcgacat cctgccggag cgtgcttcca
      661 agggccaggc catccgttac ctcgcctaca aatggggctt gtcgctcaag cagttcgtgg
      721 tcgccggtga ttccggcaac gacgaagcca tgctggtggg cgacacgcta ggtgtcgtcg
      781 tcggcaacca cagccccgag ctggaatgcg tacgtgggct ggaccaggtg tatttcgccg
      841 aggcgcacta cgcagccggc atcgtcgagg gcttacgcca ctatcgcttc attgaagaac
      901 attccgaacc tgcccaggag gaaacagccg catgatccaa acgctgaccg aattcaccga
      961 acaccaccgg gacgccatct acaccctgct acgccgttat ttcgcgctgg atcggcaact
     1021 gttgctgcaa tccgacatgc ggggcgaact tgatgcgttg aaagcgcaac tgaagctgga
     1081 cgaggccgcg ctcaaaccgc tggtcgattt cgtcacccag ttgcacgagg gcgtgatgat
     1141 ctcgccctgg gcctatttca cggtgcgacc ggcagtgggg caattcgatt acctgcgtat
     1201 ccaccaggac aacctgatcc cggagcgcgt cagcccggcc gagttcctca tgttcaagga
     1261 acagctggcg caccacgcac gcccggcgtc gagcctggag atcgatttcg ggccgttcag
     1321 ccgcgaattc cccaaactca gggagacgcg ctccatcggc cagggcctgc tgttcctcaa
     1381 tcgccagctg tcgagcggca tgttcacgca cccgcagcgc ggtgccgaga aacttctgca
     1441 cttcctcggc gtgcatacca tcgaggggca acagatgatg ttcctcagca cgccgcgcga
     1501 caccgcgacc gcccccagcg tgctgcgcag cgctatcgat tttctggaaa cacaggacga
     1561 aacgacgccg tggacggcgt tcgccgaccg cttcgccaag ctcggtttcg cgccgggttg
     1621 gggcaacaat gccgggcgag tggccgagac catgaacctg ctcatggata tcctcgaagc
     1681 accttcgccg gccgcgctcg aaagcctgct ggcgcgtatc cccatgatct cgcgcatcct
     1741 catcgtctcg ccgcatggct atttcggcca ggccaacgtg ctcggcctgc cggacaccgg
     1801 cggacaggtc gtctatatcc tcgaccaggt acgcgcgctg gagaaggaga tgcgcgagcg
     1861 tctgctggaa cagggcgtcg aagtcatgcc caagatcctg atcggcaccc gcctcatccc
     1921 tgattccgcc ggcaccacct gcagccagcg gctggagaag gtcaatggct gcgagaacac
     1981 ctggatcctg cgcgtgccga tgcagcgcgc caatggcgag atcgtgcagc aatggatctc
     2041 gcgcttcgag atctggcctt acctggagga ttattcctac cagctggatc gcgaggcgct
     2101 ggccgaattc agcgggcgcc ccgatctcat catcggcaat tattccgacg gcaatctggt
     2161 ggcttcgttg ctgagccagc ggctcaatgt cacgcagtgc aacatcgcgc acgcgctgga
     2221 aaagaccaag tacctgcatt ccgatctctt ctggcaggat agcgacgaca aataccattt
     2281 ctcgtgccag tacacggccg acctcatggc catgaactgc gccgatttca tcatcaccag
     2341 cacctaccag gagatcgccg gcaccgagca tatcgaaggc cagtacgaga gctaccgcac
     2401 cttcagcatg cccgggctat accgtgtgat caacggcgtc gatatcttcg accccaagtt
     2461 caacatcgtc tcgcccggcg ccaacgccga tgtctatttc ccctactcgg cggaagagcg
     2521 gcgtctgtcg agcctgtggc ccgagatcga gaacatgatc tacggcggcg ccatgccatc
     2581 ggcgcgcggg gtgctcaagg acaagaagaa accgctgatc ttcaccatgg cgcggctcga
     2641 ccgcatcaag aacctggccg gcctggtcga gtggtatggc cgctcagaag atttgcgcaa
     2701 gcgcgccaac ctgctcgtgg tcggcggtta tgtggacgcc agccactcga gcgaccacga
     2761 agagcaggcg cagatccgcc agatgcatgc gctgatggac cagtacaagc tcgacggcga
     2821 gatgcgctgg ctcggcatgc gcctcgacaa gaaccagtcg ggcgagctct atcgctatgt
     2881 cgcagaccgc cacagcgtgt tcgtacaacc agccctgttc gaagccttcg gcctcaccat
     2941 catcgaagcc atggcatccg gcctgccgac cttcgccacc tgctacggcg ggccgcgcga
     3001 gatcatccag cacaatcgct ccggtttcca catcaacccg aatgaaggcg aagccgcggc
     3061 ggcgctgatc agcgatttct tcgatcgctg caaggccaac ccggatgaat ggcaacgcat
     3121 ctccgacggc gcgctggcgc gcgtcgcctc gcattacacc tgggagaaat atgcgcagcg
     3181 cgtgatgacg ttgtcgcgca tctacggctt ctggaaattc gtcagtgggc tggagcgcca
     3241 ggagacggcg cgctacatcg atatgttcta tcacctgcag ttcagaccgc tggccgaacg
     3301 tatcccccgc tgaatcaacc ataaggagac ccgatgacca cctattttgc agacttcaac
     3361 ctggccaccc tgcgggagct gatgcacctg tccgcgccag ctttccatat cctcatcatc
     3421 ctcgtgctgg cggcgatcgc gttccgcctc tcccgcaggc tgatccgcgt tctcaaggag
     3481 ttcatgctgc agcgcgccga ggataatccg gaggaattca agcgcatcga aacgctgggg
     3541 cgggtgttcc gctatatcac ttcggtcgtc atcaccatcg ttgcgggcat gctgatcctg
     3601 agcgagctcg gcatctccat cgctccgatc ctcgccgctg ccggcgtcct cggtctggcc
     3661 atcggcttcg gcgcccagag cctggtcaag gattacttca gcggcttctt tctgctgctg
     3721 gagaaccaga tccgtcaggg cgacgtagtc aacgtcgccg acaagggcgg ctttgtcgag
     3781 gagatgacgc tacgttacgt caagatgcgc gactacgaag gcaatgtgca tttcatccct
     3841 aacggcatca tcaccaccgt caccaacatg agccgccagt tcgcctatgc cgtgatcgat
     3901 atcggcgtca gctatcgcga agacgtggat gaagtgatcg cggtcatgga acaggtgggt
     3961 aaggaattgc gcgaggacga agcgctggcg cccaagatcc tcgccaacat cgagatcgcc
     4021 ggggtagacc agttcgccga ctcgagtgtg atcatcaaat gccggttcat ggtgcaggca
     4081 ctggaacaat ggacagtgaa gcgcgcctac ctgaagcgca tcaaggaagt gttcgacgag
     4141 cgaggcatcg agattccgtt cccgcatctg actatctacc cgggcgtgct gaaggacggc
     4201 agcgccccag cgctgcgcat cggtgccgcg caggaagcga tgcagacgcc atcgtgatac
     4261 cgagcccggc tcgatcatgc gcacgaatat gacaacccga ccctgataag gagaggccca
     4321 tgatgaaccc gatcagaatg ctgcgttacg tcacgcatcg cagcgatacc tggcataccc
     4381 tggccgcaga acagccgagc gtgatgaaga tgctgtggag ctatatcgtc cccatgtcct
     4441 tgatcccgcc ggtgatgctc aacatcgttg cacgcaatca ccccaggata ttcatggata
     4501 tcctgcctgg cgaccggccg ttgatcgtcg gcctcatcct gttcctggcg ctggtcgtcg
     4561 gcgtactggt gatggcctgg atcagcaagt tcctggccga gatggtcgat ctgaagccgt
     4621 cataccgcga tgcgcttttg gtcatcgccg tcgccgccac gccgttctgg gtagcttcga
     4681 tctgctacct ggtcccgaac ctagcattga acctggtgct gcacggcgtg gccggcgtct
     4741 tctcggtcgc cctggtctat cagggcatcc tcacgatctt ccatctccac cgacgcggcg
     4801 ccgcggccat gctcaccacc gccatcggct gcactggcgc gatcggcttc ggcgtgctgc
     4861 tggtcgggat gctgatgaca tggggcgtgg tgcaggactt gcagttcgcc gtcaaagcct
     4921 gacggctttg ctgtcacacc ctcaagaata acggccgcga tcgcggccgt tttcgcatcc
     4981 aatcgtcact ggatttgatc tatgtcaatt gtgatcaaaa ttatccttgc tagcatcaga
     5041 cccactgccg catggcattc actttgatgt acgacaagga ttgccgatgg cctcacaaaa
     5101 attaagaatt ctggtagcag gcagtctgtc cctgctgttc ccgcaagccg tgttcgccga
     5161 ccaggcgact tcaccgcaac c
//
LOCUS       DATMTW010000297         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_428816, whole genome shotgun sequence.
ACCESSION   DATMTW010000297 DATMTW010000000
VERSION     DATMTW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428816"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VN084_05780"
     CDS             <1..1245
                     /locus_tag="VN084_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWT29001.1"
                     /translation="FNEPLTRVLCIQASTPDDWKLTTIGWIFSLAIVALGLSAAFAGK
                     WLEEVGPRRTMFVAACCFGGGLVLSAIGVHTHQIWLLYLGYGVIGGCGLGLGYVSPVS
                     TLIKWFPDRRGMATGMAIMGFGGGAMIGSPLSVALMKHFESATSVGVAETFLVMGVLY
                     FISMTIGSLAIRIPPEGWRPEGWTPKEKPKKLVTQNNVHIDEALKTPQFYLLWVMLCM
                     NVTAGIGILSQASVMIQEMFKGGITPEAAAGFVGLLSVFNMGGRFIWSSASDYLGRQM
                     TYFIFFALGAVLYVMLPGFSHAGNVVLFIAASAIILSMYGGGFSTIPAYLADIFGTRY
                     VGGIHGRLLTAWATAGVLGPVLVNYIREYQLDHGMAKADAYTTTLYIMAALLAVGFIS
                     NLLMRPVDPKHHLKDDSPATAN"
     gene            1256..1384
                     /locus_tag="VN084_05785"
     CDS             1256..1384
                     /locus_tag="VN084_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29002.1"
                     /translation="MSDHHDHPVMRTALLVLFWAYVLIPLAWGVFETMKKAMALFA"
     gene            complement(1432..2511)
                     /locus_tag="VN084_05790"
     CDS             complement(1432..2511)
                     /locus_tag="VN084_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWT29003.1"
                     /translation="MINVNLYPRWLVAVDDAEAREINVFALLQGIADCGKLTQAARDA
                     GISYRHAWQLLDQWGKYFDSPLVEKRQGHGVTLTALGEALLWAEKRVNARLTAQLSNL
                     ASELQSELNRVIHDTKQIVRMHASHGYAVAELRDFLHASTQTQLDLQYRGNFDALYDL
                     ARGDCDIAGFHIPEGSLQQAAILKYEKWMNTPDNRVVHFVRRTQGLILQKGNPLQITG
                     IADLVRNGVRLVNREPSSGTRMLFDMLCHEAGIEASGINGYRREELTHNAVGALIASG
                     MADVGFGVEAAAREFGLDFIPVAAEQYYLIGHKDTLLRPALQSILELLRSDAFKRAII
                     AIPGYSAPRSGEIETSQAIYTRIAA"
BASE COUNT          545 a          866 c          833 g          607 t
ORIGIN      
        1 ttcaatgaac cactgacgcg ggtattgtgc atccaggcct cgacgcctga cgactggaag
       61 ctcaccacca tcggctggat cttcagtctc gccatcgtcg cactcggttt gtctgcggcc
      121 tttgccggca aatggctgga agaggtcggc ccgcgccgca ccatgttcgt cgctgcctgc
      181 tgcttcggcg gtggcctcgt gctgtccgcc atcggtgtgc atacccacca gatctggctg
      241 ctctacctcg gttatggggt gattggcggc tgtggcctcg gcctgggtta tgtttcaccg
      301 gtctccacgc tcatcaagtg gttcccggat cgccgcggca tggcgaccgg catggccatc
      361 atgggcttcg gcggcggcgc catgatcggc tcgccgctga gtgtcgcgtt gatgaagcat
      421 ttcgaatccg ccacctcggt cggtgtcgcc gagaccttcc tggtcatggg cgtgctctac
      481 ttcatctcca tgaccatcgg ctcactggcc atccgcatcc cgcccgaggg ctggagaccc
      541 gaaggctgga cccccaagga gaaaccgaag aaactggtca cgcagaacaa cgtgcatatc
      601 gacgaagccc tgaagacgcc gcagttctat ctgctgtggg tgatgttatg catgaacgtg
      661 actgccggta tcggcatcct cagccaggca tcagtgatga tccaggagat gttcaagggc
      721 ggcatcacgc cagaagctgc ggccgggttc gtgggactgt taagcgtgtt caacatgggc
      781 gggcgtttca tctggtcttc ggcatcggac tacctcggtc gccagatgac ctatttcatc
      841 ttcttcgcgc taggcgcggt gctttacgtc atgctgccgg gcttcagcca tgccggtaac
      901 gtcgtcctgt tcatcgccgc gtctgcgatc atcctcagta tgtacggcgg cggcttctcg
      961 accatcccgg cgtaccttgc cgatatcttc ggcacacgct acgtcggggg catccacgga
     1021 cgcctgttga cggcatgggc cacggcaggc gtgctggggc cggtgctggt gaactacatc
     1081 cgtgaatacc agctcgacca cggcatggcc aaggccgacg cctacaccac aacgctgtat
     1141 atcatggcgg cactactggc ggtgggcttc atcagcaacc tgctgatgcg ccctgtggac
     1201 ccgaaacatc atttgaaaga cgacagtccg gcgactgcga actaaggatc agaccatgag
     1261 cgatcatcac gaccaccccg tcatgcgcac cgcactgctg gtgctgttct gggcctatgt
     1321 gctcatcccg ttggcatggg gcgtattcga gaccatgaag aaagcgatgg cgctgttcgc
     1381 ctgaacactg ccatcgggcc tgatccaggc cgattcaacc cgtccaggcg gtcacgcggc
     1441 gatgcgcgtg tagatcgcct ggctggtctc gatctcgccg gagcggggcg ccgaatagcc
     1501 cggaatggca atgatggcgc gcttgaaggc gtcgctacgc aatagctcga ggatactctg
     1561 caaggctggc cgcagcaggg tgtccttgtg gccgatcagg taatactgtt ctgccgccac
     1621 cgggatgaaa tcgagcccga actcgcgtgc ggctgcctcg acgccgaacc ccacatcggc
     1681 catgccgctc gcgatcaagg cgccgacagc attgtgcgtg agctcttcgc gtctgtagcc
     1741 attgatgccc gaggcctcga tgccggcctc atggcacagc atgtcgaata gcatacgcgt
     1801 acctgaactg ggctcgcggt tgaccagcct caccccgttg cgaacgagat cggcgatgcc
     1861 cgtgatctgc agcggattac ctttctgcag gatcagcccc tgggtacgac gcacgaaatg
     1921 cacgacccgg ttgtccgggg tattcatcca cttctcgtat ttcaggatcg ccgcttgctg
     1981 cagtgagcct tcagggatgt ggaaaccggc gatatcgcaa tcgccgcgcg ccagatcata
     2041 gagcgcatcg aagtttccac ggtactgcag atcgagctgt gtctgcgtac tggcatgcag
     2101 gaaatcccgc agttccgcca ccgcgtaacc atggctggca tgcatgcgca cgatctgctt
     2161 ggtgtcatgg atgacgcgat tgagctcaga ctgtagctcg gaggcaagat tgctgagttg
     2221 ggccgtgagg cgcgcgttga cgcgcttctc tgcccaaagc agcgcctccc ccagggcggt
     2281 gagcgtgacc ccatgtccct ggcgcttttc caccagcggg ctatcgaaat atttccccca
     2341 ttgatcgagc agttgccagg catggcgata tgagatgccg gcatcgcgtg cggcttgcgt
     2401 cagcttgccg caatcggcga tgccttgcag cagggcgaag acattgatct cgcgggcttc
     2461 ggcatcatcg accgccacca gccagcgagg gtagagatta acattgatca tatgagatta
     2521 agaacatatg ttgtgtgggg atggatttca tattttcacc gtgccaatga ttgcataaaa
     2581 tgcctgccgt accaatatct taagttaaca acccgcgaat ttttacatat gttgatattt
     2641 gcatatgtcc tagcccttac ggaacccagc atcgtccatg gataaaaacg ccaaagtcac
     2701 tgattaccac caccccgccg gcggctgggg tgcgctcgtc agcaccgcca agcacctgat
     2761 gttccagggc atccccatca agggcgcgat gacgctgctc aagaacaacc agaagcatgg
     2821 tttcgattgc cctggctgcg cctggcccga c
//
LOCUS       DATMTW010000298         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_630504, whole genome shotgun sequence.
ACCESSION   DATMTW010000298 DATMTW010000000
VERSION     DATMTW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630504"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /gene="glmM"
                     /locus_tag="VN084_05795"
     CDS             complement(<1..1231)
                     /gene="glmM"
                     /locus_tag="VN084_05795"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWT29004.1"
                     /translation="MSRKYFGTDGVRGRVGEAPITPDFVMRLGYAAGRVLTNIDTHTG
                     SANPTVLIGKDTRISGYMLEAALEAGLSAAGVDTLLTGPMPTPAVAYLTRALRAQVGI
                     VITASHNPYEDNGIKFFSAEGTKLPDAIEFAIEAELDQPMVVMPSAKLGKARRLDDAA
                     GRYIEFCKSTFPNALDLRGLKIVLDCANGATYHVAPPVFHELGAEVVAIGNQPDGLNI
                     NQDCGSTHTKALCKAVVEHKADLGIAFDGDGDRVMMVDAKGKLLDGDQLLYIIAMHRK
                     TRGELGGGIVGTLMTNLALEHALAKADIPFARAKVGDRYVLELLNQNAWQVGGENSGH
                     ILALDKHSSGDGIIAALQVLNAMRASGKSLAELGASLTLYPQVLVNVKVKKRIEISGN
                     PVLQKAVKDAESELNGSG"
     gene            complement(1403..2239)
                     /gene="folP"
                     /locus_tag="VN084_05800"
     CDS             complement(1403..2239)
                     /gene="folP"
                     /locus_tag="VN084_05800"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWT29005.1"
                     /translation="MTIAPRFHCGRYALDLSRPHIMGIVNVTPDSFSDGGKFVAVDNA
                     VAHAMQLVEDGADILDIGGESTRPGAVPVSLEDELRRVIPVIEALAKVTRVPLSIDTY
                     KPQVMQAALDAGASLINDIRALQEPGALEIAASTDAGICLMHMQGLPATMQVDPRYDD
                     VVAEVRAFLAERMAAAEAAGIASERILLDPGFGFGKRTEHNIALLQALPQLLDLGRPL
                     LVGLSRKSVLGQLTGGDVSVRLHSSIAASVLSVMKGARIVRVHDVKATADALKVVAAV
                     LD"
BASE COUNT          442 a          660 c          707 g          446 t
ORIGIN      
        1 tgccgctacc gttcaattct gactcggcgt ccttgacggc tttctgcagg accgggttgc
       61 ccgagatttc gatacgcttt ttcactttga cgttgaccag cacctgcgga tacagggtca
      121 ggctggcgcc aagttcggca agggatttgc cgctcgcgcg catggcgttc agcacctgca
      181 gggcggcgat gatgccgtca ccgctactgt gcttgtccaa agcgaggata tggccggagt
      241 tctcgccacc gacttgccag gcattctgat tcagcagttc cagcacatag cggtcgccga
      301 ccttggcacg tgcgaacggg atatccgcct tggccagggc atgctccagt gccaggttgg
      361 tcatgagggt accgacgatg ccgccgccca gttcgccacg cgtcttgcga tgcatggcga
      421 tgatatagag cagctggtca ccgtcgagca acttgccctt ggcgtcgacc atcatgacgc
      481 ggtcaccgtc gccatcgaac gcgataccga ggtcggcctt atgttcgacc acggccttgc
      541 acaacgcttt ggtgtgggtc gagccgcagt cctgattgat gttgaggcca tccggctggt
      601 tgccgatggc gacgacttcg gcgccgagtt cgtggaacac cggcggggca acatggtagg
      661 tcgcgccgtt ggcgcaatcc agcacgatct tcaggccgcg aagatccagc gcgttgggga
      721 acgtgctctt gcagaattcg atatagcggc cggccgcatc gtcgaggcga cgtgccttgc
      781 ctagcttggc ggaaggcatg acgaccatgg gctggtcgag ttcggcttcg atggcgaact
      841 cgatggcgtc tggcaatttg gtaccttcgg cagagaagaa cttgatgcca ttatcttcgt
      901 agggattgtg cgaagcggtg atgacgatac cgacttgcgc gcgcaaagca cgcgtcaggt
      961 acgccacagc gggcgtcggc atggggccgg tgagcagggt atcgacaccg gctgcggaaa
     1021 gcccggcttc gagggctgct tccagcatgt atccggagat gcgggtgtcc ttgccgatca
     1081 gcaccgtcgg gttggcgctg ccagtgtggg tatcgatgtt ggtcaagacg cggccagcag
     1141 cgtagccgag ccgcatgacg aaatccggcg tgatcggggc ctcgcccacg cgaccacgga
     1201 caccatcggt accaaaatat tttctactca tcttttatgc tcttaaataa ccgcgaatca
     1261 acgcaaatgc gcgccaatca ggcaaacacc gcattgtaat acttgccgca cagccccagc
     1321 cagtaatgct aaagaataaa acggatgcac tggaacatca gctccaggtt tatccgcatt
     1381 catcggcggc tgacaggcgt tttcaatcca gcaccgcagc gactaccttg agggcatccg
     1441 cggtggcttt cacgtcatgc acacgcacga ttctagcgcc tttcatcacc gacagcacgg
     1501 aagctgcgat actcgaatgc aagcgcacac tgacgtcgcc gcccgtgagt tggcccagta
     1561 cggatttgcg cgacaggccg accagtagcg ggcggccgag gtcgagcagc tggggcaggg
     1621 cctgcagcag cgcgatattg tgttctgtgc gcttgccgaa gccgaagccc ggatcgagca
     1681 agatgcgttc gctggcgata cctgccgcct cggcggccgc catgcgctca gcgaggaagg
     1741 cacggacctc ggccacgaca tcatcgtatc tgggatcgac ttgcatggtc gcaggcaggc
     1801 cttgcatgtg catgagacag atgccggcat cggtactggc ggcgatctcg agtgcgcccg
     1861 gctcctgcag ggcacggata tcgttgatga ggctggcgcc ggcatcgagg gccgcctgca
     1921 tcacttgtgg cttataggta tcgatcgaca gtggcacccg ggtcacttta gcgagcgcct
     1981 cgatcaccgg gatcacacgg cgcagttcgt cttcgagcga gacaggtacg gcgccagggc
     2041 gggtcgattc accaccgata tcgaggatgt cggcaccgtc ttccaccagc tgcatggcat
     2101 gcgcgacggc attgtcgacc gctacaaact tgccgccgtc tgaaaacgaa tcgggcgtga
     2161 cattgacgat gcccatgatg tggggccgcg aaaggtcgag cgcatatcga ccgcagtgga
     2221 accggggagc aatggtcatg tcagttcagt cggat
//
LOCUS       DATMTW010000299         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_293680, whole genome shotgun sequence.
ACCESSION   DATMTW010000299 DATMTW010000000
VERSION     DATMTW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293680"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VN084_05805"
     CDS             complement(<1..417)
                     /locus_tag="VN084_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HWT29006.1"
                     /translation="MSALTFKLKQALAQRVDCAPLTPDLLAGKSLTEIAAIELQCGRF
                     KLRADSVFAISGDDASEIIFEQGSAKLDSIGQGMSQGRITVKGDAGSYLGLQMKKGEI
                     VVHGNADAFVASGMAGGMIQVHGDVGDFLAAALPGDR"
     gene            complement(414..1322)
                     /gene="fhcD"
                     /locus_tag="VN084_05810"
     CDS             complement(414..1322)
                     /gene="fhcD"
                     /locus_tag="VN084_05810"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HWT29007.1"
                     /translation="MQINGVHIDETFAEAFGMRGTRVIITAQNHKWARNAAAAMTGFA
                     TSVIACGVEAGIERELSEDETPDGRPGISVLLFAMGSAVLMKQLETRMGQCVLTCPTT
                     AAFSGIDEGYPEITRINLGKNLRFFGDGFQISKQFGGKRYWRVPVMDGEFMTEETTGM
                     VRAVGGGNFLILAESQPQALAACEAAIEAMRKMPNVIMPFPGGVVRSGSKVGAKYKGM
                     IASTNDAFCPTLKGITKTELSPEIESVMEIVIDGLTAEDINRAMRAGITAVCELGAAQ
                     GIKRISEGNYGGKLGQFHFKLREIMA"
     gene            complement(1467..3143)
                     /locus_tag="VN084_05815"
     CDS             complement(1467..3143)
                     /locus_tag="VN084_05815"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HWT29008.1"
                     /translation="MLIKLSGGTVYDPAHQIDGKVMDLYVRDGRIVERPYDHEKIDQE
                     YDLRGKVVMSGAIDMHTHIGGGKVNIARTMLPEDHRADPAIHTEFCRAGCGHAAPSTL
                     TTGYRYAEMGYTAGFEPAMMAVNARQTHLEMGDTPIIDKGGYAMIGSDDYFLRMLAAK
                     KDQNAINDYVAWTLHASQAMGIKVVNPGGISAFKFNQRALDLDELNVHYQVSPREVLK
                     ALSRAVHELGIPHPLHVHGNNLGVPGNVETTLKTMGASEGYPMHLTHIQFHSYGTEGD
                     RKFSSGAAAIAEAINKHKHISADVGQILFGQTVTASGDNMRQFANAGHASPSKSVLMD
                     IECDAGCGVVPFKYKDQNFVNALQWAIGLEIFLLVDDPWRIFLTTDHPNGAPFTSYPH
                     LIRLLMDKSFRNDMFTKLNLDAQAMSTLSSLDREYSLYEIAIMTRAGAAKLVGLHDRG
                     HLGAGAAADITVYTDDADREKMFSKPDYVFKDGELVVRNGQVVKVTWGTTHTVKPEFD
                     RGIEKDLKQYFARYHTMDMEHYKISADEIADGGRGRVQVHECKGRREKTE"
     gene            complement(3144..>3904)
                     /locus_tag="VN084_05820"
     CDS             complement(3144..>3904)
                     /locus_tag="VN084_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HWT29009.1"
                     /translation="GTREVVYLGGRDLDTRAGVSPKGKLPEVLPCDLARLPEVLAALR
                     AIAAGKPVKADAIAGIPAAALVSLAGRLKAAKYGVIAWSASALDFPHAELAIQSITGL
                     VADINQTTRCAGLPLSGNEGDLGAYNTSAWISGYPLRSSYRRDHPDYDPYHYEGARML
                     AEGEADVLLWINSYSPKRLPADGRVPDIVIGHPATVFEQAPAVFIPVATPGIEIKGIQ
                     FRSDSSVALPLKQLCQTTLPALADVLAAIESGLR"
BASE COUNT          684 a         1146 c         1283 g          791 t
ORIGIN      
        1 gcggtcgccg ggcagggcgg cggcgaggaa atcaccgaca tcgccatgca cctggatcat
       61 gccacccgcc ataccgctgg cgacgaaagc atcggcattg ccatgaacga cgatctcgcc
      121 ttttttcatc tgcaggccca ggtaagagcc ggcatcgccc ttgacggtga tgcgaccttg
      181 cgacatgccc tggccgatac tatcgagctt ggcgctgccc tgctcgaaga tgatctcgct
      241 ggcgtcatcg ccgctgatgg cgaacacgct gtcagcacgg agtttgaagc gcccgcattg
      301 cagttcgatg gccgcgatct cggtgaggga tttccccgcg agcaggtcag gcgtgagcgg
      361 cgcgcaatcg acacgctgcg ccagtgcttg cttgagtttg aaggtcaatg cgctcatgcc
      421 atgatctccc gcaacttgaa gtggaactga ccgagcttgc cgccatagtt gccctccgat
      481 atgcgcttga tgccctgggc cgcgcccagt tcgcagactg cagtgatgcc ggcgcgcatg
      541 gcgcggttga tatcttcggc ggtgaggcca tcgatgacga tctccatcac cgattcgatc
      601 tcgggcgaga gctcggtttt ggtgatacct ttcagtgtgg ggcagaaggc atcgttggtc
      661 gaggcgatca tgcccttgta cttggcgccg accttggagc cggatcgcac cacgccgccc
      721 gggaacggca tgatgacatt gggcatcttg cgcatggctt cgatcgcggc ttcgcaggca
      781 gccaaggctt gcggctggga ttcggcgagg ataaggaaat tgccgccgcc gaccgcgcgt
      841 accatacccg tggtctcctc ggtcatgaat tcgccgtcca tcaccggcac gcgccagtag
      901 cgcttgccgc cgaactgctt ggagatctgg aagccgtcgc cgaagaagcg cagattcttg
      961 ccgaggttga tgcgcgtgat ctcggggtag ccttcgtcga taccggagaa cgccgctgtg
     1021 gtcgggcagg tcagcacgca ctggcccatg cgggtctcga gttgcttcat gagcaccgcg
     1081 ctacccatgg cgaacagcag caccgagatg ccgggacgac cgtccggggt ctcgtcctcc
     1141 gatagttcgc gttcgatgcc ggcctcgaca ccgcaggcga tcaccgaggt ggcgaagccg
     1201 gtcatggcgg ctgccgcatt gcgtgcccat ttgtggttct gggcggtgat gatgacgcgc
     1261 gtgccgcgca tgccgaacgc ttcggcgaag gtctcgtcga tgtgtacgcc gttaatctgc
     1321 ataggcgcct ttccaaactg tcatgagcag tgccgagcac aagtcgccgc ggagtgagtt
     1381 gtgagacata tcaatgagat aggcaaacag cgaacgagca tgcaacgccg tgaccggctt
     1441 gcgcaataag tttgtaagga tgcctatcat tcggttttct ctcggcgacc tttgcattca
     1501 tgcacctgca cacggccacg accgccgtca gcgatctcgt cggcgctgat cttgtagtgc
     1561 tccatatcca tggtgtgata gcgggcgaag tactgcttga ggtctttctc gatgccacgg
     1621 tcgaattccg gcttgaccgt gtgcgtcgtg ccccaggtga ccttgaccac ctggccgttg
     1681 cgcaccacca gttcgccgtc cttgaagacg taatccggct tggagaacat cttctcgcgg
     1741 tcagcatcgt cggtgtagac cgtgatatcg gccgcggcac ccgcgcccag gtggccacgg
     1801 tcgtgcaggc ccaccagctt ggccgcgcct gctcgggtca tgatggcgat ctcgtacagg
     1861 ctgtattcgc gatcgaggct ggagagcgtg ctcatggcct gcgcatcgag gttgagcttg
     1921 gtgaacatgt cgttgcggaa gctcttgtcc atgagcaggc ggatcaggtg tggatagctc
     1981 gtgaacggcg cgccattggg gtgatcggtg gtgaggaaga tccgccaggg gtcatccacc
     2041 agcaggaaga tctcgagacc gatggcccat tgcagggcat tgacgaagtt ctggtccttg
     2101 tacttgaacg gcaccacgcc gcaaccggcg tcgcactcga tatccatcag caccgacttg
     2161 ctggggctgg catggccggc attggcgaac tgccgcatgt tatcgccgga agcggtgacg
     2221 gtctggccga acaggatctg gccgacgtcg gccgagatgt gcttgtgctt gttgatggct
     2281 tcggcgatgg cggcggcgcc agaggagaac ttgcggtcgc cctccgtccc gtaactgtgg
     2341 aactggatat gcgtcaggtg catgggatag ccttcggagg cacccatggt cttcagcgtg
     2401 gtctcgacgt tgcccggcac gccaaggttg ttgccgtgca cgtgcagcgg atgcgggata
     2461 ccgagctcat gtacggcgcg cgacaaggcc ttgagcacct cacgtggcga gacctggtaa
     2521 tgcacgttga gttcgtcaag gtcgagcgcg cgctggttga acttgaaggc gctgatgcca
     2581 cctgggttga ccaccttgat gcccatggcc tggctcgcgt gcaaggtcca ggcgacgtaa
     2641 tcgttgatgg cgttctggtc ttttttcgcc gccagcatgc gcaggaagta atcgtcgctg
     2701 ccgatcatgg cgtagccacc cttgtcgatg atgggcgtat cgcccatctc gaggtgcgtc
     2761 tgacgcgcgt tgacggccat catcgcgggc tcgaagcctg cggtatagcc catctcggca
     2821 tagcggtaac cggtggtcag ggtcgaaggc gcggcgtggc cgcagccagc gcggcagaat
     2881 tcggtatgga tggccgggtc ggcacggtga tcctcgggca acatggtgcg ggcgatattg
     2941 accttgccgc caccgatgtg ggtgtgcatg tcgattgcac cggacatgac gaccttgccg
     3001 cgcaagtcgt attcctggtc gatcttctcg tggtcataag ggcgttcgac gatgcggccg
     3061 tcacggacgt agagatccat gaccttgcca tcgatctgat gggccgggtc atagacagtg
     3121 cctccggaga gtttgatgag catttatcgc aatcctgatt cgatggcagc caggacgtcg
     3181 gccaaggccg gcagcgtcgt ctggcacagt tgcttgagtg gcagcgcgac tgagctatcg
     3241 ctgcggaact ggatgccctt gatctcgatg ccgggcgtgg cgacgggaat gaacaccgcc
     3301 ggcgcctgct cgaacaccgt cgcgggatgg ccgatgacga tgtcaggcac gcggccgtct
     3361 gcaggcaagc gcttcgggct gtagctattg atccagagca ggacgtcggc ttcgccttcc
     3421 gccagcatgc gcgcaccctc gtaatggtaa gggtcgtaat ccggatgatc gcggcgatac
     3481 gaactgcgca acggataacc gctgatccag gcgctggtgt tataggcgcc gaggtcacct
     3541 tcgttgccgc tcaggggcaa gcctgcgcaa cgtgtggtct ggttgatgtc ggcgaccagg
     3601 ccggtgatgc tctgaatggc gagttcggca tgcgggaaat ccagcgcgct ggcggaccat
     3661 gcgatgacac cgtatttcgc ggccttgagg cggcctgcca gcgagacgag ggctgccgcg
     3721 gggatgcctg cgatggcgtc ggctttcacc ggcttgccgg ccgcgatggc ccgcaacgcc
     3781 gccagcactt ccggtagacg cgccagatcg caaggcagca cctcgggtaa cttgcccttg
     3841 ggagagacgc cggcgcgcgt atcgaggtcg cggccaccca ggtagacgac ctcgcgcgtg
     3901 cccg
//
LOCUS       DATMTW010000300         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_362684, whole genome shotgun sequence.
ACCESSION   DATMTW010000300 DATMTW010000000
VERSION     DATMTW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362684"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="pyrE"
                     /locus_tag="VN084_05825"
     CDS             complement(<1..581)
                     /gene="pyrE"
                     /locus_tag="VN084_05825"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWT29010.1"
                     /translation="MSDFTQEFIQFAIQKQVLRFGEFKTKAGRNSPYFFNAGLFNDGD
                     SLMRLGEFYAAAILQSGIRFDMLFGPAYKGITLAASIAIALARQGVNVPFAYNRKEAK
                     DHGEGGTIVGAPLQGRVLIVDDVISAGTSVRESVEIITAAGAIPAGVAIAIDRQERGL
                     GALSAVQEVERDHGLPVCAIANLDGLIRYLHDQS"
     gene            597..1385
                     /locus_tag="VN084_05830"
     CDS             597..1385
                     /locus_tag="VN084_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWT29011.1"
                     /translation="MDLLRIISLNLNGIRSAANKGLYAWLKAQDADIVCMQEIKAQAG
                     DMTREMLQPEGYYGYFHYAEKKGYSGVGIYAKKQPDAIIEGLGMADIDAEGRYIEARF
                     GNLSVVSLYLPSGSSGEERQTFKFDVMARILPHLEALKASGREVVICGDWNIAHKEAD
                     LKNWRGNKKNSGFLPEERAWLTDLFGRVGWVDVYRLLYPDTLDECYTWWSNRGQALAK
                     NVGWRIDYQIATPGIAATAKQASIYKAERFSDHAPLVIDYDSAT"
     gene            complement(1374..2153)
                     /locus_tag="VN084_05835"
     CDS             complement(1374..2153)
                     /locus_tag="VN084_05835"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWT29012.1"
                     /translation="MRNWVIGSLSCCLLWATPNVQACSVSSTDQRVSLLELYTSEGCS
                     SCPPADQWLSRLQAAGYPTDKVIPLALHVDYWDYIGWKDRFARPEFTARQRQAASRDL
                     ASFVYTPQVLLNGRDFRGWQQGNRFVQALEAYRSQAARVQLRLDVTPPPSGQYRLKLD
                     AKALDADSRKQGDVYIAVYENGLKTKVNAGENSGRELSHDHVVREWRGPYRLQDATGA
                     SWESLIMLDKSWQGRDAGVAAFVQNRSSGEVLQAVATDLCG"
     gene            complement(2242..3534)
                     /locus_tag="VN084_05840"
     CDS             complement(2242..3534)
                     /locus_tag="VN084_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HWT29013.1"
                     /translation="MTDSTTLRQALFNRRMLICIFTGFSSGLPLYILISLLPAWLRSE
                     GVDLKAIGLFALIGLPFTWKFLWAPFFDRYSPLGFLRGIGRRRGWLLISQTALLLSIP
                     AFGAFDPKLDIWTIAYLAGVVAFFSASQDVVIDAYRRELLPDMELGLGNAIHVNAYKI
                     AGLIPGSLSLILADLMPWDLVFLITALFMLPGLLMTFMVKEPQLLAGAPRTLRQAVVE
                     PFHEFMTRHGWRYAAMILAFLFFYKLGDSMAVALATPFYLDMGFSKTEIGLIAKNAGL
                     WPSVIGGLLGGVWMVKLGINRALWVFGVLQMIAILGFAWLAWLGPFPAPGAAELTELA
                     IVIGIEAFGVGLGTTAFVAFIARTTHPLYTATQFALFTSLMAVPRTFANALTGYLVDW
                     LGWAAFFLFCFAIAIPGMLMLIKVAPWNAAEPRFGERS"
     gene            3627..4292
                     /locus_tag="VN084_05845"
     CDS             3627..4292
                     /locus_tag="VN084_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWT29014.1"
                     /translation="MLEYSLTAALLVGLLGGGHCAGMCGPIVGAVTVTMPGNRPRLDL
                     VLVYNLGRILTYTLAGALAGAIGASSYFLDHILPVQQLLYGLASLMLIVLGLYLAGIW
                     HGVTAIEGIGRHLWRYLQPLSGKLLPVRHLPQALGLGLVWGWLPCGLVYSVLVAAIAT
                     GDPLKGASLMLAFGLGTLPALMAMGMAAVRLKRWLQDLWVRRISGLTVLAFGVVGLVR
                     LWQ"
     gene            complement(4320..4937)
                     /gene="metW"
                     /locus_tag="VN084_05850"
     CDS             complement(4320..4937)
                     /gene="metW"
                     /locus_tag="VN084_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HWT29015.1"
                     /translation="MNTRTEKAMPITEFRQDFAIIADWVTLGAKVLDLGCGDGSLLHF
                     LRGSLDVRGYGVEKDDANWLQCIDKQLNVIQMDLEDGLSGFEAQSFDVVVLSQTLQAM
                     HNTEGIVQEMLRVGREAIVTFPNFGYWRSRSQIAAGQMPVSKELPYQWYDTPNVHLCT
                     IKDFDEFCVNHRIHVMERRVLTNGKLVTFMPNLLGSLAMYRLRNS"
     gene            complement(4934..6112)
                     /locus_tag="VN084_05855"
     CDS             complement(4934..6112)
                     /locus_tag="VN084_05855"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWT29016.1"
                     /translation="MSEIASVGIVQSQVAHFTEPLALKSGETLPAYDLVYETYGTLNA
                     DRSNAVLICHALSGNHHVAGRYQQTDKHPGWWDNLVGPGKPLDTDRFFVIGLNNLGGC
                     HGSTGPSSINPATGQPWGAAFPLVTVEDWVTSQARLADRLGIHQLAAVVGGSLGGMQA
                     LQWAIAYPERLRHALVIASAPNLTAQNVAFNEVARQAIITDPEFYGGDYYAHGTVPRR
                     GLRIARMLGHITYLSDDAMGEKFGRKLRAGDIQYSFDVEFEMESYLRYQGDKFAGEFD
                     ANTYLRMTRALDYFDPALLHGGDLSRALAAVRAKFLVVSFTSDWRFSPARSREIVKAL
                     LDNELSVSYAEVTAAHGHDAFLMPDPHYHAILRSYLDHIALEQTPSSEHAQAINGGAA
                     "
     gene            6149..>6381
                     /locus_tag="VN084_05860"
     CDS             6149..>6381
                     /locus_tag="VN084_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWT29017.1"
                     /translation="MPPHISSYQGISPRIGPDVYIHDTAVVIGDVQLAQDVSVWPTVV
                     IRGDVNSIHIGAGSNVQDGSVLHVNHRSAEDPAG"
BASE COUNT         1230 a         1908 c         2070 g         1173 t
ORIGIN      
        1 gattgatcgt gcaagtatcg gatcaggccg tcgaggttgg cgatggcgca gactggcagg
       61 ccgtggtcgc gttccacctc ctggaccgcg gacagcgcgc cgaggccgcg ctcctgccgg
      121 tcgatggcga tcgccacgcc agcggggata gcgccggcgg cggtgatgat ctccacggat
      181 tcgcgtaccg aggtgccagc cgatatcacg tcgtcgacga tcagtacacg gccctgcaac
      241 ggcgcaccga cgatggtgcc accttcgccg tggtccttgg cttccttgcg gttgtaggcg
      301 aacggcacgt tgacgccctg gcgggcgagc gcgatggcga tgctggccgc cagcgtgatg
      361 cctttgtaag ccgggccgaa cagcatgtcg aagcggatgc cggattgcag gatcgccgcg
      421 gcgtagaact cccccagccg catcaggctg tcgccatcgt tgaacagacc ggcgttgaag
      481 aaatacgggc tattacgacc ggccttggtt ttgaattcac cgaagcgcaa gacctgcttt
      541 tggatggcga attggataaa ctcttgggtg aaatcagaca taacgctcga aaaggaatgg
      601 atttgctaag aattatatcg ttaaacctga acggcatccg ctctgccgcg aacaagggtc
      661 tctacgcatg gctaaaggca caggatgccg acatcgtctg catgcaggag atcaaggcac
      721 aggcaggcga catgacgcgc gagatgctgc agccggaagg ctattacggc tacttccact
      781 acgccgagaa aaagggctac agcggcgtag gtatctatgc caagaaacag cctgacgcca
      841 tcatcgaagg tctgggcatg gcggatatcg atgccgaagg ccgttacatc gaagcgcgct
      901 tcggcaatct gagcgtggtc tcgctgtacc tgccgtcagg ctcgagcggt gaggagcgcc
      961 agaccttcaa gttcgacgtc atggcgcgta tcctgccgca tcttgaagcc ctcaaggcca
     1021 gcggccgcga ggtggtgatc tgcggcgact ggaatatcgc gcacaaggaa gccgatctca
     1081 agaactggcg cggcaataaa aagaattccg gtttcctgcc ggaagagcgg gcctggctga
     1141 cggatctctt cggccgggtc ggctgggtag acgtctaccg cctgctgtac ccggacacgt
     1201 tggacgaatg ctatacgtgg tggagcaacc gtggccaagc cttggccaag aacgtgggct
     1261 ggcgcatcga ttaccagatc gccacgcccg gcatcgcagc aaccgcgaag caagcgtcga
     1321 tctataaggc ggaacgcttc agcgatcacg cccctctggt catcgactac gattcagcca
     1381 cataggtcgg tcgcgactgc ctgcaatacc tcgccgctgc tgcggttctg cacgaaagcg
     1441 gccacgcccg catcgcggcc ctgccatgat ttatccagca tgatcagcga ctcccagctg
     1501 gcgccggtcg catcctgcag ccggtatggg ccgcgccatt cgcgcaccac atgatcatgg
     1561 ctgagttcgc gaccgctgtt ctcgccggca ttgaccttgg tcttgaggcc attctcatag
     1621 acggcgatat acacatcgcc ctgcttgcgg ctatcggcat cgagtgcttt ggcatcgagc
     1681 tttaagcgat actgcccgga tggcgggggt gtgacgtcca gccgcaactg gacgcgcgca
     1741 gcctggctgc ggtatgcctc tagggcctgg acgaagcgat tcccttgttg ccagcccctg
     1801 aagtctcgac cattgagcag cacttgcggg gtatagacga aactcgccag gtcgcgactc
     1861 gctgcctggc gctggcgtgc ggtgaattcg ggacgcgcga agcgatcctt ccagccgatg
     1921 taatcccagt agtcgacatg cagcgccagc ggaatgacct tgtccgtagg atagccggcg
     1981 gcctgcaggc gacttagcca ttgatcggca ggcgggcagc tgctgcaacc ctccgaagtg
     2041 tagagctcga gcaggctgac gcgctgatcg gtgctgctga cgctgcaggc ctgcacatta
     2101 ggtgtggccc agagcaggca acacgacagg ctgccgatga cccagtttcg catgatgttc
     2161 tccgctgtgt tgatgctcgt tggtcgtggc agctggcgcc accttacatc aacgagccag
     2221 cgcgcaggcg catgccgcag gtcaggaacg ttcgccgaaa cgcggctccg ccgcgttcca
     2281 tggcgccacc ttgatcagca tcagcatgcc ggggatggcg atggcgaaac agaacaggaa
     2341 gaaggcggcc cagcccagcc agtcgaccag ataaccggtc aaggcattgg cgaaggtgcg
     2401 cggcacggcc atcaggcttg tgaacagcgc gaactgtgtg gccgtgtaga gcgggtgcgt
     2461 ggtgcgcgcg atgaacgcga cgaaagccgt ggtgccgagg ccgaccccga aagcctcgat
     2521 gccgatgacg atggccagtt cggtgagctc cgccgcgccc ggcgccggga aaggcccgag
     2581 ccacgcgagc caggcgaaac cgaggatggc gatcatctgc agcacgccga acacccacaa
     2641 ggcgcgattg atgccaagct tgaccatcca cacgccaccc agcagcccac cgatcacgct
     2701 gggccagagc cccgcgttct tggcgatgag gccgatctcg gtcttgctga agcccatgtc
     2761 gagatagaac ggcgtcgcca gcgccacagc catgctgtcg ccgagtttgt agaagaacag
     2821 gaaagccagg atcatggctg cgtagcgcca gccgtggcgc gtcatgaatt cgtggaacgg
     2881 ttcgaccacc gcctggcgta gcgtgcgcgg cgcgccggcg agtagttgcg gttccttgac
     2941 catgaacgtc atcaacaggc ctggcagcat gaacagcgcg gtgatcagga acacgagatc
     3001 ccagggcatg aggtctgcca ggatcagcga cagcgagcct ggaatgagac cggcgatctt
     3061 gtaggcgttg acgtggatgg cgttgcccag ccccagttcc atgtccggca ggagttcgcg
     3121 gcgataggcg tcgatcacga catcctgcga ggcgctgaag aacgcgacca ccccagcgag
     3181 gtaagcgatg gtccagatat ccagtttagg gtcgaatgcg ccgaaggccg ggatcgatag
     3241 cagcaaggcg gtctgcgaga tcagcagcca gccgcggcgg cggccgatgc cgcgcaggaa
     3301 gccgagcggc gagtagcggt cgaagaacgg cgcccacagg aatttccagg tgaagggcag
     3361 gccgatgagc gcgaacaggc cgatggcctt gagatccacg ccttccgaac gcagccatgc
     3421 aggtaacagg ctgatcagga tatagagcgg taaccccgag ctgaagccgg tgaagatgca
     3481 gatcagcatg cgccggttga agagggcctg cctgagcgta gtggaatcgg tcatggccgc
     3541 taccatacct gaaatcccgc acggcccaaa tccttgcgca ccagaaatcg ccttacgaca
     3601 tcgctgtgcc catgccacaa tgacgcatgc tcgaatattc cctgactgcg gccttgctcg
     3661 tcggcctgct tggcggcggc cattgcgccg gcatgtgcgg ccccatcgtc ggtgccgtca
     3721 ccgtcaccat gcccggcaac cgtccgcggc tcgatctggt gctggtctac aacctcggtc
     3781 gtatcctgac ctataccctg gctggcgcgc tggccggcgc gatcggcgcc agcagctact
     3841 ttctcgatca tatcctgccg gtgcaacagc tcctgtacgg gctggcgagc ctcatgctga
     3901 tcgtgctcgg gctttacctg gcaggcatct ggcacggagt gacggcgatc gaggggatcg
     3961 gccggcacct gtggcgctat ctgcaaccct tgtccgggaa actgttgccg gtgcgtcatc
     4021 tgccgcaggc gctaggtctg gggctggtct ggggctggct gccctgcggc ctggtctaca
     4081 gtgtgctggt ggcggccatc gccacgggcg acccgctcaa gggggcctcg ctgatgctgg
     4141 ctttcgggct cggaaccttg cctgcgctga tggccatggg catggcggca gtgcggctca
     4201 agcgctggct gcaggatctc tgggtgcggc ggatcagcgg attgacagtg ctggcgttcg
     4261 gggtggtcgg cttggtgcgc ttgtggcagt gagcaggcgg cctgtgcttg aggccccgct
     4321 caggaattgc gcaggcgata catcgcgaga ctacccagca agttgggcat gaaggtcacg
     4381 agtttgccat tggtcagcac gcgacgttcc atgacgtgga tgcggtggtt gacgcagaat
     4441 tcgtcgaaat ccttgatcgt gcacaggtgc acgttgggcg tgtcgtacca ctggtagggc
     4501 agttccttcg ataccggcat ctggcccgcg gcgatctgcg agcggctgcg ccagtaaccg
     4561 aaattgggga aggtgacgat ggcttcgcgg ccgacgcgca acatctcctg cacgatgcct
     4621 tcggtgttgt gcatggcctg gagggtctgc gagagcacga ccacgtcgaa ggactgggct
     4681 tcaaacccgg acaacccgtc ttccaggtcc atctggatga cgttgagctg cttgtcgatg
     4741 cactgcagcc agttggcgtc gtctttttcc acaccatacc cgcgcacatc gagcgacccg
     4801 cgcaggaagt gcagcagcga gccgtcaccg catcccaggt ctagtacctt ggcgcccaat
     4861 gtcacccagt ctgcgatgat cgcgaagtcc tgccggaatt ccgtgatcgg catagctttt
     4921 tctgtgcgtg tattcatgcc gcgcccccgt tgatggcctg tgcgtgctcg ctcgaaggcg
     4981 tctgttccag cgcgatgtga tccaggtaac tgcgcaggat ggcgtggtaa tgcggatccg
     5041 gcatcaggaa cgcatcatgg ccgtgcgcgg cggtcacttc ggcatagctt accgacagct
     5101 cgttatccag cagcgccttg acgatctcgc gcgaacgcgc cggcgagaat cgccagtcgc
     5161 tggtgaacga gaccaccagg aatttggccc gcacggcggc caatgcccgg ctgagatcac
     5221 cgccatgcag cagcgcagga tcgaaataat cgagcgcgcg cgtcatgcgc aggtaagtgt
     5281 tggcgtcgaa ctcgccggcg aatttgtcgc cctggtagcg aaggtaggac tccatctcga
     5341 attcgacatc gaagctgtac tgaatgtcgc cagcacgcaa cttacggccg aatttctcgc
     5401 ccatggcgtc atccgacagg taggtgatat ggcccagcat gcgcgcgatg cgcaggcccc
     5461 gacgcggcac cgtgccgtgg gcataatagt cgccgccgta gaactcgggg tcggtgatga
     5521 tggcctggcg agcgacttca ttgaaggcga cgttctgtgc cgtcaggttg ggggcagagg
     5581 cgatcaccag tgcatgccgc aggcgttccg ggtaggcgat cgcccattgc agtgcctgca
     5641 tgccgcccag actaccgccg acgaccgcgg ccagctggtg gatgcccaga cgatctgcga
     5701 ggcgggcctg tgaagtcacc cagtcttcga cagtgacgag cgggaacgca gccccccagg
     5761 gttggccggt ggccggattg atcgaggagg ggccggtcga gccatggcaa ccaccaagat
     5821 tgttgaggcc gatgacgaag aaacggtcgg tgtccagagg cttgcccggg ccgaccaggt
     5881 tatcccacca gcctggatgt ttatcggtct gctggtagcg accggcgacg tgatggttgc
     5941 ccgagagcgc gtgacagatg agcaccgcgt tggatctgtc ggcattgagc gtgccgtagg
     6001 tctcgtagac caggtcgtac gcaggcaagg tctcgccgct cttcagcgct agcggttcgg
     6061 tgaagtgcgc gacctgcgat tgaacgatgc cgacggatgc gatttctgac atggcccgaa
     6121 ttatacacgg acggctatac tacggcttat gccgccccat atctccagct atcagggtat
     6181 cagcccgcgc atcgggccag acgtctatat ccacgatact gccgtcgtca tcggcgacgt
     6241 ccagctcgcg caggatgtct cggtgtggcc gaccgtggtg atccgcggtg acgtcaacag
     6301 catccatatc ggcgctggca gcaatgtgca ggacggttcg gtgctgcatg tcaatcatcg
     6361 ttcggcagag gacccggccg g
//
LOCUS       DATMTW010000301         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_563446, whole genome shotgun sequence.
ACCESSION   DATMTW010000301 DATMTW010000000
VERSION     DATMTW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563446"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..737
                     /gene="clpP"
                     /locus_tag="VN084_05865"
     CDS             111..737
                     /gene="clpP"
                     /locus_tag="VN084_05865"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWT29018.1"
                     /translation="MSMQSQWEPQGLGYIPMVIEQSGRCERAYDIYSRLLKERVIFLV
                     GPVNDMTANLVVAQLLFLEAENPDKDISLYINSPGGSVTAGMAIYDTMQFIKPDVSTL
                     CIGQAASMGSLLLTAGAKGKRYCLPNSRVMIHQPLGGFQGQASDIEIHAKEILYLREK
                     LNGILANHTGQPVKTIERDTDRDNFMSAEQSVAYGLVDKVIANRADAA"
     gene            775..2043
                     /gene="clpX"
                     /locus_tag="VN084_05870"
     CDS             775..2043
                     /gene="clpX"
                     /locus_tag="VN084_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWT29019.1"
                     /translation="MTTKADGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDLCND
                     IIREEVQDENGLKNRDNSLPTPKEICTILDQYVIGQTQAKKNLAVAVYNHYKRLDQTA
                     NKDDVEIAKSNILLIGPTGSGKTLLAQTLARLLDVPFVMADATTLTEAGYVGEDVENI
                     MQKLLQKCDYEIEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTV
                     ASVPPQGGRKHPNQEFVQLDTTNILFICGGAFDGLEKIIRARSEKGGIGFGAEVKSKD
                     DARAVGEVLRDVEPEDLIKFGLIPEFVGRLPAVATLESLDEAALMKILVEPKNALTKQ
                     YTKLFKMEGVDLEFRDSGLELIAKKALERKTGARGLRSIMEHALLEIMFELPSLPNLS
                     KVVVDDGVISGDTPPILIYSEQARLAESAS"
     gene            2164..>4309
                     /gene="lon"
                     /locus_tag="VN084_05875"
     CDS             2164..>4309
                     /gene="lon"
                     /locus_tag="VN084_05875"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWT29020.1"
                     /translation="MTEQTLPAFSPDSDLLPLLPLRDVVVYPHLVIPLFVGRAKSVRA
                     LELASEHNKQILLVAQKSPNEDEPTAADLYHVGTIATVLQMLKLPDGTVKVLVEGVHR
                     AKVSEFVETSDCFAAKAERIEHAPQDDNETQALMRTVFTQFDQYVKLNKKIPPEILTS
                     LATIDDAGRLADTITAHLSLKLEEKQSILEMFSVAERLEHLLSLLEAEIDILQVEKRI
                     RGRVKRQMEKSQREYYLNEQVKAIQKELGEQDENADIEELEKRIKSARMPKEALTKVN
                     AEMKKLKMMSPMSAEATVVRNYIETLVNLPWKKKTKISQDLLAAEAVLDADHYGLEKV
                     KERIVEYLAVQQRVDKLKAPILCLVGPPGVGKTSLGQSIAKAVNRKFVRVALGGVRDE
                     SEIRGHRRTYIGSMPGKVLQSMTKVGVKNPLFLLDEVDKMGQDFRGDPSSALLEVLDP
                     EQNHTFVDHYVEVEYDLSDVMFVATANSLNIPAPLLDRMEVIRLAGYTEDEKVNIAMR
                     YLLPKQLKTHGLKETEIAISEATIRDVVRYYTREAGVRSLDREISKISRKVVKELLTT
                     KSKSAKAPRKVTVSPKNLEKYLGVHRFDYGVAAKDNQVGQVTGLAWTEVGGELLTIES
                     VLLPGKGKVITTGKLGEVMQESIQAAMSVVRSRAKRLGIAEDFYEKNDIHIHLPEGAT
                     PKDGPSAGIAITTALVSVLANIPVRADVAMTGE"
BASE COUNT          995 a         1219 c         1263 g          832 t
ORIGIN      
        1 gacgagccgg ctgcgctggc gaccgaagag aacgtcgtta gctgggtgct ggaagctgcc
       61 aaggtgaccg acaagaaagt caaattcgac gacctgatgg gaaatgcgtg atgagcatgc
      121 aaagccagtg ggaaccacaa ggcctggggt atatccccat ggtcatcgag cagagcggtc
      181 gttgcgaacg cgcctatgac atctattccc gcctgctgaa ggagcgggtc attttcctgg
      241 tggggccggt caatgacatg actgccaacc tggtcgtggc gcagctgttg ttcctggaag
      301 cggagaatcc ggacaaggat atctcgctct acatcaattc cccaggcggc tcggtgacag
      361 ctggcatggc gatctacgac accatgcagt tcatcaagcc agacgtgagc accctgtgca
      421 tcggccaggc tgcgagcatg ggctccctgc tattgaccgc gggtgccaaa ggcaagcgtt
      481 attgcctgcc caactcccgc gtcatgatcc accagccgct cggcggtttc cagggccagg
      541 cctcggatat cgagatccac gccaaggaga tcctctactt gcgtgaaaag ctcaacggta
      601 tcctggccaa ccataccggt cagccagtca agaccatcga gcgcgacacg gatcgcgaca
      661 acttcatgag cgctgaacag tcggtggctt atggcctggt ggacaaggtc attgccaacc
      721 gtgcggatgc cgcataacat tcataacgtc tgtatcaagt tctaggatag tttcatgacg
      781 accaaggccg atggcgaaaa attactgtat tgctcctttt gcggtaagag ccagcatgaa
      841 gtcaggaaac tgatcgcagg tccctccgta ttcatctgcg atgaatgtgt cgatctgtgc
      901 aatgacatca tccgcgagga agtgcaggac gagaacggcc tcaagaatcg cgacaatagc
      961 ctgccgacgc cgaaagagat ctgcactatc ctcgaccagt atgtgatcgg tcagacccag
     1021 gccaagaaga atctcgcggt ggccgtctac aatcactaca agcgcttgga ccagactgcg
     1081 aacaaggatg acgtcgagat cgccaagagc aacatcctgc tgatcgggcc gaccggctcc
     1141 ggcaagacct tgctggcgca aacgctggcc aggttgctcg atgtgccatt cgtcatggcc
     1201 gatgccacga ccctgaccga ggccggctat gttggcgaag acgtcgaaaa catcatgcag
     1261 aagcttctgc aaaagtgtga ttacgagatc gaaaaggcac agcgcggcat cgtttacatc
     1321 gatgagatcg ataagatctc gcgcaaatcg gacaacccgt ccatcacgcg tgacgtatcg
     1381 ggcgagggcg tacagcaggc cctgctgaag ctgatcgaag gtacggtcgc ttctgtgccg
     1441 ccacagggcg gtcgcaagca cccgaaccag gagttcgtgc agcttgatac caccaacatc
     1501 ctattcattt gcggcggtgc gttcgacggt ctggaaaaga tcatccgtgc ccgttcggaa
     1561 aagggcggta tcggtttcgg ggccgaagtg aagagcaagg acgatgcccg tgcggtaggc
     1621 gaagtcctgc gtgatgtcga gcctgaagat ctgatcaagt ttgggctgat ccctgaattc
     1681 gtcggccgtc tgccggccgt cgccacgctg gaatcgctgg acgaagcggc gctcatgaag
     1741 atcctggtcg agcctaagaa cgccctgacc aagcagtaca ccaagctgtt caagatggag
     1801 ggcgtcgatc tcgagttccg cgattctggg cttgaactca tcgccaaaaa agcgctagaa
     1861 cgaaagactg gcgcgcgcgg cctgcgatcc atcatggagc atgccttgct cgagatcatg
     1921 ttcgaactgc cttcgttgcc gaacttgagc aaagtggtcg tcgatgacgg cgtcatcagt
     1981 ggcgacacgc ctcccatttt gatttattct gaacaagcac gattggctga atctgccagc
     2041 taacacctga tccagccagt ctgtgttttt gcagatggct acttgaatcg tccgagtcgg
     2101 tccccatctg caagttatgc ggcgcggtat cttgccgtgc catatcaaac ttagggtcta
     2161 ctcatgacag aacaaacttt accggctttc tccccagact ccgatctact gccgctgttg
     2221 ccgctgcgcg acgtggtggt ctatccgcat ctggtcattc cgctgtttgt tggccgggcc
     2281 aagtctgtcc gtgcactgga actggcatcg gaacacaaca agcagatcct gctggtcgcc
     2341 cagaagtcac cgaacgaaga tgagccgacc gcggctgatc tctatcatgt cggcaccatc
     2401 gctaccgtgt tgcagatgct gaagttaccc gacggtaccg tcaaggtgct ggtcgagggc
     2461 gtacatcggg ccaaggtcag cgagttcgtc gagaccagcg actgctttgc cgccaaagcg
     2521 gagcgcatcg agcatgcacc gcaggacgac aacgagaccc aggcgctcat gcgtaccgta
     2581 ttcacgcagt tcgatcagta cgtcaagctc aacaagaaga tcccgccgga gatcctgacc
     2641 tcgctcgcga ccatcgacga tgccgggcgc cttgccgata ccatcaccgc acacctctcc
     2701 ctcaagctgg aagagaaaca gagcatcctt gagatgttca gcgtggctga gcgcctggaa
     2761 catctgctca gcctgctcga ggccgagatc gatatcctgc aggtggaaaa gcgtatccgc
     2821 ggccgcgtca agcgccagat ggagaagagt cagcgcgagt attacctcaa cgaacaggtc
     2881 aaggctatcc agaaagagct gggcgagcag gacgagaacg ccgacatcga agagctggag
     2941 aagcgcatca agtcggcacg catgccgaag gaggcgctga ccaaggtcaa cgccgagatg
     3001 aagaagctga agatgatgtc gccgatgtcc gctgaagcga ccgtcgtgcg caactacatc
     3061 gagaccctgg tcaacctgcc atggaagaag aagaccaaga tcagccagga tctgctggcg
     3121 gcggaagcag tactcgatgc cgatcattac ggtctggaaa aggtcaagga acgtatcgtc
     3181 gagtaccttg cggtacagca gcgtgtggac aaactgaagg cgccgattct ctgtctcgta
     3241 gggccgccag gcgtcggcaa gacctcgctc ggccagagta tcgccaaggc ggtcaatcgc
     3301 aagttcgtgc gtgtcgccct gggtggcgtg cgcgatgaat ccgagatccg cggtcaccgc
     3361 cgtacctata tcggctccat gcccggcaag gtgctgcaaa gcatgaccaa ggtcggcgtc
     3421 aagaatccgc tgttcctgct cgacgaagtc gacaagatgg gccaggattt ccgcggcgac
     3481 ccatcgtcgg ccttgctcga agtgctcgat cccgagcaga accatacctt cgtcgatcat
     3541 tatgtcgaag tcgaatacga cctgtctgac gtcatgttcg tcgcgaccgc caactcgctg
     3601 aatatccctg cgccgttgct ggatcgtatg gaagtgatcc ggcttgctgg ctacaccgaa
     3661 gacgagaagg tcaacatcgc catgcgttac ctgctgccca agcagttgaa gacccatggc
     3721 ctcaaggaga ccgaaatcgc tatctcggaa gcgacgattc gcgatgtcgt gcgctattac
     3781 acccgcgaag ctggggtgcg tagcctggac cgcgagatct cgaagatctc gcgcaaggtg
     3841 gtcaaggaac tgctgaccac caagagcaag tccgccaagg cgccgcgcaa ggtcacggtc
     3901 tctccgaaga atctggagaa atatctgggc gtacatcgtt tcgactacgg cgtcgcggcc
     3961 aaggacaacc aggtcggtca ggtgaccggt ctggcctgga ctgaggtcgg cggcgaattg
     4021 ctgaccatcg agtccgtgct gttgccgggc aagggcaagg tcatcaccac cggcaagctg
     4081 ggcgaagtca tgcaggagtc catccaggcg gcgatgagcg tcgtgcgtag ccgtgccaag
     4141 cgtcttggca tcgctgagga tttctacgag aagaacgaca tccacatcca tttgccggaa
     4201 ggcgctactc cgaaggatgg tcctagtgcg ggcatcgcca tcactacggc gttggtttcg
     4261 gtgctagcca atataccggt tcgggccgat gtcgcgatga caggtgaga
//
LOCUS       DATMTW010000302         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_93952, whole genome shotgun sequence.
ACCESSION   DATMTW010000302 DATMTW010000000
VERSION     DATMTW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93952"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1545
                     /gene="amt"
                     /locus_tag="VN084_05880"
     CDS             34..1545
                     /gene="amt"
                     /locus_tag="VN084_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWT29021.1"
                     /translation="MPAQSEEATAGPAVPYHQAYTRFESVVKPLQVAAAETPAAPTEE
                     VVVEQEATSETPTAATEEAEQPAVAEPLATPVPAPTPADPAEEAAAPSGPHMVAADQI
                     SAGDTAWMMTSAALVLLMTIPGLALFYAGMVRKRNVLSTTLQSFTICCLVTIVWVVAG
                     YSLAFTPNNLYIGGFDRLMLDGLAFLNADGQLSVSHLATSIPESVFIMFQLTFAIITP
                     ALITGAFAERIRFPAMVVFMVLWSLLIYAPVAHWVWEPGGWLASQGVLDFAGGTVVHI
                     NAGVAGLMCAYVLGPRHGYGKEPFPPYNLIYTLIGASLLWVGWFGFNAGSAVASDGRA
                     GMAMLVTQVATAAAVLTWMAIEWIRRSRATLLGACSGAIAGLVAITPASRFIDPKGAL
                     IIGVAAGLFCYWGATGLKRVLGADDALDVFGIHGVGGIVGAILTGVLANKAIGGADGD
                     PMVQCIGVIATLLYSGIGTLIILKLIDWTIGLRVSTEDEQDGLDISQHAERVL"
     gene            1545..1883
                     /locus_tag="VN084_05885"
     CDS             1545..1883
                     /locus_tag="VN084_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29022.1"
                     /translation="MTEAEKTGIAAHLYVSLRRSANRVIDVEWMSRNEEYAREVIQLA
                     RQQGSEEIRHYADRYEALLSGAPAPIPASPAKVAVAETKTDVGQLPVFEEPAEEDLSA
                     TASRYIGALR"
     gene            1958..2155
                     /locus_tag="VN084_05890"
     CDS             1958..2155
                     /locus_tag="VN084_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HWT29023.1"
                     /translation="MQETNGETVQSPCVGICAMSEESGYCLGCYRTIDEIRNWWDMTD
                     AERKDTVSACEQRQIDLAVFD"
     gene            complement(2369..3817)
                     /locus_tag="VN084_05895"
     CDS             complement(2369..3817)
                     /locus_tag="VN084_05895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT29024.1"
                     /translation="MDKDKIIPLTKSAESSQEADFRIVAEDAPVMLWLTNAEGKVVFT
                     NSKWKRFVGREKQPGQVGGDAWIEALHPDDLAACMHIFNEAFANHQHFDMEYRLRRAD
                     GQYRYVLDTGEPYINKEGKFAGFIGSSTDITDRKNHENQLRLSQLEMAQHNREMQFIN
                     ELNSYLQVCRSLEETHAIIQHYARKIFADYSGVLYLFDENHATVEAVVHWGDEEHPPA
                     QFIAPDDCWGLRQGKLHSADNTDNVIVCNHIQGPCEHGYVCAPVIAQGEMIGVLSVIL
                     GDGREGAVPRSPESVTRLIAMAADNLAMALVSLKLREALRSRSVRDPLTTLFNRRYLE
                     ETLTRELSNSQRNNQNLGLIMLDIDHFKHYNDTHGHDAGDFVLQQIAELLRSKLRLGD
                     IACRYGGEELIMVMPGATKQITANRAELMRAAIAQHEFVYKGKPLDRVTASFGVSNYP
                     TDGEHGETLIKRADEALYDAKAAGRNCVKLTE"
BASE COUNT          724 a         1185 c         1235 g          875 t
ORIGIN      
        1 cttgggctgg cggcacttgg cgtcatcatg cccatgccgg cgcagtccga ggaggcgact
       61 gcaggccctg cggtacctta ccaccaggct tatacccgct tcgaaagcgt tgtgaaaccc
      121 ttgcaagtgg cggccgcgga aacgcctgcg gcaccgacag aggaagtggt tgtagagcag
      181 gaagcgacat cggagacgcc gacagcagca acggaagagg ctgagcagcc ggccgtcgcc
      241 gaacccctgg ccacgcccgt cccggcacct acgccggcgg atcctgccga ggaggcagcc
      301 gcgccgagtg gaccgcatat ggtggcggcc gaccagatca gcgcaggcga cactgcctgg
      361 atgatgacct ctgccgcgct cgtgctgctg atgacgatcc cgggtctggc tttgttctat
      421 gccggcatgg tgcgtaaacg taacgtgctc tccacgacct tgcagagctt cacaatctgt
      481 tgcctggtga ccatcgtctg ggtcgtcgcc ggttacagtc tcgcattcac gccgaacaac
      541 ctctacatcg gcggtttcga tcgcctgatg ctcgatggtc tggcctttct caatgccgat
      601 gggcaattgt ccgtcagcca tcttgccacc agcattccgg aatcggtctt catcatgttc
      661 cagctgacct ttgccatcat cacgccggcc ctgatcacgg gtgcattcgc cgagcgtatc
      721 cgtttcccgg cgatggtggt gttcatggtg ctgtggtcct tgctgatcta cgcgccggtg
      781 gcgcattggg tatgggaacc cggcggctgg ctagcgagcc agggcgtgct cgacttcgcc
      841 ggcggcactg tggttcacat caatgccggc gtggccgggc tcatgtgtgc ctatgtactg
      901 gggccacgcc atggctacgg taaggagcca ttcccgcctt ataacctgat ctacacgctg
      961 atcggggcct ccctgctgtg ggttggctgg ttcgggttca atgcgggctc ggccgtcgct
     1021 tcggatgggc gtgccggcat ggcgatgctg gtgacccaag ttgcgaccgc ggcggctgtg
     1081 ctgacctgga tggcgatcga atggatccgt cgttcccgcg cgacgctgct cggcgcctgc
     1141 tcgggcgcga tcgccgggct agtggcgatc acgccggcct caaggttcat cgaccccaag
     1201 ggcgccttga tcatcggcgt agccgctggc ctcttctgtt attggggcgc aaccggcctc
     1261 aagcgtgtcc tgggtgcgga tgacgcgctg gatgtgttcg gcatccacgg tgtcggcggc
     1321 atcgtcggtg cgatcctcac cggcgtgctg gccaacaagg ccattggcgg ggccgacggc
     1381 gaccccatgg tgcagtgcat cggcgtcatc gccaccttgc tctatagcgg tatcggtacc
     1441 ctgatcatcc tcaagctgat cgactggacc atcggtctgc gcgtatcgac ggaagacgaa
     1501 caggacggtc ttgatatctc ccaacatgct gaaagggtac tgtgatgact gaagccgaaa
     1561 agaccgggat cgctgctcac ctgtatgtga gcctgcggcg cagcgccaac cgcgtgatcg
     1621 atgtcgaatg gatgagccgc aacgaggaat acgcgcgtga ggtgatccag ctggcgcgtc
     1681 agcaagggtc tgaggaaatc aggcactatg cagaccgtta cgaagcgctg ctgtccgggg
     1741 ctccggcacc catcccggcg tctccggcca aggttgcggt cgctgaaacc aaaaccgatg
     1801 tcggccagct gccggtgttc gaggagccgg cagaggagga tctgtcggcg acggcgagcc
     1861 gctacatcgg tgccttgcgc tgaatcacgc gcaacgccgg tggccgcttt tcgctataat
     1921 gccgctttgg cttttttgaa tcggtgaagc gatgagcatg caggaaacca atggcgagac
     1981 ggtccaatcc ccttgtgtcg gcatctgtgc catgagtgaa gagagcggtt actgtctcgg
     2041 ttgttaccgg acgatcgacg agatccgtaa ctggtgggat atgactgatg ccgaacgcaa
     2101 ggacacggtc agcgcctgcg agcaacgcca gatcgatctc gcggtattcg actaaagcac
     2161 agccttggcg gtgcaccaca aagaaccggc gtttgccggt ttttttgttt caatgcttca
     2221 ttgagcgatg tgcatgttgt gacgtcgcag tcgttttcta gcgcgcagcc agcagtggac
     2281 tgcacctgca gccgaacttg tgcgctcatc ttgcgcttca tacgaccagt gatcaggatg
     2341 cctgctcggc tgcgcctcta ttgaggggct attcggtcag tttcacgcag tttcgcccgg
     2401 cagccttggc gtcgtacagg gcttcgtcgg cacgcttgat cagggtctca ccgtgctcac
     2461 cgtccgtcgg gtaattggag acgccaaaag aggcggtgac tctgtccagc ggttttccct
     2521 tgtagacgaa ttcatgctgt gcgatggccg cacgcatcaa ctccgcgcgg ttcgccgtga
     2581 tctgtttcgt cgcgcccggc atcaccatga tcaactcctc accgccataa cggcacgcga
     2641 tatcgccgag gcggagcttg gatcgcagta gctcagcgat ctgctggagc acgaagtcac
     2701 cggcgtcatg accatgggta tcgttgtagt gcttgaaatg atcgatatcc agcatgatga
     2761 gtcccaggtt ctggttgttg cgctggctgt tcgataattc gcgcgtcagg gtctcttcca
     2821 ggtagcggcg gttgaacagt gtcgtcaagg ggtcgcgcac cgagcgactg cgcaatgcct
     2881 cgcgcagttt cagactgacc agcgccatgg ccaggttatc ggccgccatc gcgatcagtc
     2941 gcgtcaccga ttccggtgag cgcggcaccg cgccctcgcg gccgtctccc agaatcacgg
     3001 agagcacccc gatcatctca ccctgggcga tcaccggcgc gcaaacgtag ccgtgttcac
     3061 aaggcccctg gatgtgatta cacacaatga cattatccgt gttgtcggcg ctgtgcagct
     3121 tgccctggcg caagccccag caatcgtcgg gggcgatgaa ctgggccggc ggatgctcct
     3181 catcccccca gtgcacgaca gcttcgacgg tcgcgtggtt ttcgtcgaac aggtagagca
     3241 cgcccgaata gtcggcgaat atcttgcgcg cgtaatgctg gatgatggcg tgcgtctctt
     3301 ccagcgaacg acagacttgc aggtaggagt tgagctcgtt gatgaactgc atctcgcggt
     3361 tatgctgcgc catctcaagc tgggagagcc ggagctggtt ctcgtggttc ttgcgatcgg
     3421 tgatatcggt cgacgatccg atgaatccgg cgaacttgcc ttccttgttg atatacggct
     3481 cacctgtgtc cagtacataa cgatattggc catccgccct gcgcaggcga tattccatat
     3541 cgaagtgctg atggttggcg aatgcttcgt tgaagatatg catgcaagcc gcgagatcgt
     3601 cgggatgcag ggcctcgatc caggcgtcgc cgccgacctg gcctggctgt ttttcgcgcc
     3661 cgacgaagcg tttccacttg gagtttgtga agacgacctt gccttcggca ttggtcagcc
     3721 agagcatgac gggcgcatct tcggcgacga tgcggaaatc cgcttcctgt gagctttccg
     3781 cggacttggt taacggtatg atcttgtcct tgtccataaa agtcttctcc tcgacatcta
     3841 aaagactccc cgatcgcagc aggtgccgca cagggcggca aaagaccgat ttataagacg
     3901 gattatttga tgcagacggc gcggacctgt ttcatgtccg tgacgccttg caataatttc
     3961 tcgatcccat cctgtttcag cgtgcgcatg ccgtcctcca gcgctgtggc gaacatgtc
//
LOCUS       DATMTW010000303         7058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_159509, whole genome shotgun sequence.
ACCESSION   DATMTW010000303 DATMTW010000000
VERSION     DATMTW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159509"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VN084_05900"
     CDS             complement(<1..818)
                     /locus_tag="VN084_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWT29025.1"
                     /translation="MITGKVVQCIGAVVDVEFPRDQMPKVYDALIIDEENSTAEAGLT
                     LEVQQQLGDGIVRTIAMGSSDGLSRGMKFKSTGGQISVPVGEGTLGRIMDVLGRPIDE
                     VGPIESDERRSIHQAAPTFEELSPSVDLLETGIKVIDLVCPFAKGGKVGLFGGAGVGK
                     TVNMLELINNIAKQHSGLSVFAGVGERTREGNDFYHEMAEAGVIKLDNMKESKVAMVF
                     GQMNEPPGNRLRVALSGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGR"
     gene            complement(887..1753)
                     /gene="atpG"
                     /locus_tag="VN084_05905"
     CDS             complement(887..1753)
                     /gene="atpG"
                     /locus_tag="VN084_05905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWT29026.1"
                     /translation="MANSKEIRTKIKSVENTRKITKAMEMVAASKMRKAQDRMRHARP
                     YAEKIRNVAAHMSKANSEYKHPFLIKREQVKNIGLIVVTSDKGLCGGLNTNVLRLSLN
                     QMKTWESEGKGVQVTAIGNKGFGFMGRVGAKVRSHVTGLGDAPHMDKLIGPVKVMLDA
                     YIAGEIDQLFICYTGFVNTMKQEPTMQQLLPLAGEALGSAEGHWDYIYEPDAKSVIDE
                     LMMRYVESLIFHAVAENMASEQSARMVAMKAASDNAKNVIGELKLVYNKARQAAITKE
                     ISEIVGGAAAVS"
     gene            complement(1798..3339)
                     /gene="atpA"
                     /locus_tag="VN084_05910"
     CDS             complement(1798..3339)
                     /gene="atpA"
                     /locus_tag="VN084_05910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWT29027.1"
                     /translation="MQLSTSEISELIKSRLENFSSSAEARTQGTVISVTDGIVRIHGL
                     SNVMAGEMIEFPGNTYGLALNLERDSVGAVVLGAYEHITEGDTCKCTGRILEVPVGPE
                     LIGRVVNALGQPIDGKGPVNAKITDKIEKVAPGVIARKSVSQPVQTGLKSIDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGQNMTCIYVAIGQKASTIANVVRKLEENGAL
                     EYTIVVAASASDSAALQFLAPYAGCTMGEYFRDRGQDALIVYDDLTKQAIAYRQISLL
                     LRRPPGREAYPGDVFYIHSRLLERAARVNEEYVEKFTNGEVKGKTGSLTALPVIETAA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLDRGRMFTELMKQAQYAPLSVSDMAVTLLA
                     ANKGYIDDVPVNKVLAFESALHSFMKSKYKNLLDKIESSKDLAGDDEKAVEAAIQDFK
                     ATNAY"
     gene            complement(3363..3899)
                     /locus_tag="VN084_05915"
     CDS             complement(3363..3899)
                     /locus_tag="VN084_05915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HWT29028.1"
                     /translation="MAEAVTIVRPYAVAAFRLAKEQKALAKWSEMLGFAAAVAADAQM
                     QAYIADPKVSSADQEKLFLAVAGDKLDEAGKNLIKLLVEYDRLALLPEVVAAFEELKA
                     QDEGVLEAEITAAAKPSDAEVKALVKRLESKFGKKIEASVKVDPEIIGGIKILVGDTV
                     IDASVRGQLQELAYTLKG"
     gene            complement(3913..4383)
                     /locus_tag="VN084_05920"
     CDS             complement(3913..4383)
                     /locus_tag="VN084_05920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWT29029.1"
                     /translation="MNINATLIAQAIMFAVFVWFCMKFVWPPIMNALAARQKQIADGL
                     AAADRGKHELELAAKKATQELHAAKEKASDIIAQAEKRATEIVEESKTNAKTEGDRII
                     AGAKAEIEQEVNRAKEGLRQQVSELAVAGAEKILRKEIDAKAHADMLKTIANEL"
     gene            complement(4429..4662)
                     /gene="atpE"
                     /locus_tag="VN084_05925"
     CDS             complement(4429..4662)
                     /gene="atpE"
                     /locus_tag="VN084_05925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HWT29030.1"
                     /translation="MEQALLFIAGAIMLGLGALGAAVGIGILGGRFLEGAARQPELIP
                     MLRTQFFIVMGLVDAVPMIAVGIAMYVLFAVAG"
     gene            complement(4711..5577)
                     /gene="atpB"
                     /locus_tag="VN084_05930"
     CDS             complement(4711..5577)
                     /gene="atpB"
                     /locus_tag="VN084_05930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWT29031.1"
                     /translation="MASETLTSSEYIKHHLQNLTCTINEGHLHCAHSAQEAKEMGFWA
                     INLDTVLMSLLLGVVFLFFFTRAARKASAGVPSGVVNFVEWVIEFIDSSVRGSFSFKN
                     SLVAPLALTIFMWIFLMNLMDLVPIDFFGQFIIHQIDPHMFFKIVPTTDPNATFGMAI
                     SVFFIVLFYSVKMKGLGGFIGELTLQPFGKFGLPANIFLEGVNLIAKPISLALRLFGN
                     MYAGEMIFILIAIMGSTWAGFTIANATLFSSQILLSLVWAIFHILIITLQAFIFMTLT
                     IVYLDMAHQEHH"
     gene            complement(5595..6008)
                     /locus_tag="VN084_05935"
     CDS             complement(5595..6008)
                     /locus_tag="VN084_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HWT29032.1"
                     /translation="MSGAKIEIAGKNHGLIVLGLQILLITISAGVTYLIDNPQSGISV
                     IWGEACALVNVSLLSWRMLQGKHRDATAGRQLLWMYRSALERFFVVIGLLAFGMLRLH
                     LPAGQVILGFVMGQAILMLVPLVRGLLNRSRVEVK"
     gene            complement(6077..6937)
                     /locus_tag="VN084_05940"
     CDS             complement(6077..6937)
                     /locus_tag="VN084_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWT29033.1"
                     /translation="MVKSKGLGRGLDALLAGDVGSVGEADSLRMLKVSQLQPGKYQPR
                     TQMDQESLASLAASIKAQGIMQPILVREIADDRFEIVAGERRWRASQLAGLPEVPVLV
                     REIPDDSALAMALIENIQRENLNPLEEANGIKRLIDEFAMTHQAAAEAVGRSRAAVTN
                     LLRLLNLAAPVQEMLMHNQIDMGHARALLALDPARQVMAAQQIVHKGLSVREAERLAN
                     AEQKPVKKTARPKPDRDLLRLEDIAADRVGARVSIKQGAKGAGKLVIEYRDLDQLDGI
                     LSLMGVSCRE"
     gene            complement(6930..>7058)
                     /locus_tag="VN084_05945"
     CDS             complement(6930..>7058)
                     /locus_tag="VN084_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWT29034.1"
                     /translation="NIRLAEAPSYGMPALIYDRASKGAQAYLALADEIIKRGVVNG"
BASE COUNT         1491 a         2045 c         1918 g         1604 t
ORIGIN      
        1 atacgaccca gcagtgcgga tacttcggta ccagccaggg tgtagcggta gatgttatcg
       61 acgaagaaca acacgtcacg accttcgtca cggaacttct cagccatggt cagaccggac
      121 agtgccacac gcagacggtt gcccgggggt tcgttcatct gaccgaacac catcgccacc
      181 ttggattcct tcatgttatc cagcttgatg acgccagctt ccgccatttc gtggtagaag
      241 tcattacctt cacgggtgcg ctcaccgaca ccagcgaaca cggaaaggcc cgagtgctgc
      301 ttggcgatgt tgttgatcag ttccagcatg ttcacggtct tgcctacacc agcaccaccg
      361 aacaggccaa ccttaccacc cttggcgaac ggacaaacca ggtcgatcac cttgatgccg
      421 gtttccagca ggtcaacgga aggagagagt tcttcgaaag taggggcagc ctggtggatg
      481 gagcggcgct catcggattc gatcgggccg acttcgtcga tcgggcggcc caatacgtcc
      541 atgatgcggc ccagggtacc ttcaccgacc ggtacgctga tctggccacc ggtggatttg
      601 aacttcatac cgcggctcaa gccgtcggat gaacccatgg cgatggtacg gacgatgccg
      661 tcacccagct gttgttgcac ttccagcgtg aggccggctt ctgcggtgga gttctcttcg
      721 tcgatgatca acgcatcgta cactttaggc atctggtcac gtgggaactc gacgtcgacc
      781 accgcaccga tacactgaac tactttacct gtaatcattt taaattcctt atccaaactt
      841 gattcgctga acttgatccg ctactgcttt gggtaaatcg ttatctttag gaaaccgccg
      901 ccgcgccacc gacgatttcc gagatttcct tggtaatcgc tgcctgacgg gccttgttgt
      961 acaccagctt gagttcgccg atgacgttct tcgcattatc cgaagccgct ttcatcgcca
     1021 ccatccgcgc gctctgttcg gaagccatat tctcggcgac cgcatggaag atcaacgatt
     1081 cgacatagcg catcatcaat tcatcgatca cgctcttggc atccggctcg tagatgtaat
     1141 cccaatggcc ttccgcactg cccagcgctt caccggccag cggcagcagt tgctgcatgg
     1201 tcggctcctg tttcatcgtg ttgacgaaac cggtgtagca gatgaacagc tggtcgatct
     1261 cgccggcgat ataggcatcc agcatcacct tcaccgggcc gatcagcttg tccatgtgag
     1321 gcgcatcgcc cagaccggtg acatgggatc tgaccttggc accgacacgg cccatgaagc
     1381 caaaaccctt gttaccgatg gcagtgacct gcacgccctt gccttcgctt tcccatgtct
     1441 tcatctggtt cagcgaaaga cgcaatacgt tggtattcaa accaccgcac aggcccttgt
     1501 cggacgtgac cacgatcaga ccgatattct tgacttgctc gcgcttgatc aggaacgggt
     1561 gcttgtattc ggagttcgcc ttggacatgt gcgcggccac gttgcggatc ttctccgcgt
     1621 atggacgggc atggcgcatg cgatcctgcg ccttgcgcat cttggatgca gccaccatct
     1681 ccatcgcctt ggtgatcttg cgcgtattct ctacgctctt gatcttggta cggatttctt
     1741 tgctattggc cattgttcag ttgcctgtct tttcttgcaa tcggtctcgc acgcagctta
     1801 gtacgcgttg gttgccttga agtcctggat agcagcttca acagccttct cgtcgtcgcc
     1861 agccagatcc ttgctgcttt cgatcttgtc gagcaggttc ttgtacttgg acttcatgaa
     1921 ggagtgcagg gcggattcga acgccagcac cttgttgacc ggcacgtcat cgatataacc
     1981 cttgttggcg gccagcaaag tgacagccat gtcggatacg ctcaatggtg cgtactgtgc
     2041 ttgcttcatc agttcggtga acatgcgacc gcgatccagc tgtttgcggg tcgcttcgtc
     2101 caggtcggaa gcgaactgcg cgaacgcagc cagttcacga tactgggcca gcgccagacg
     2161 cacaccgcca cccagcttct tgatgacctt ggtctgagct gcaccaccga ctcgggatac
     2221 cgagataccg gcgttgatcg cgggacggat accagcgttg aacaagtcgg tttccaggaa
     2281 gatctgaccg tcggtgatcg agatcacgtt ggtcggaacg aacgcggaaa cgtcaccggc
     2341 tgcggtttcg atcactggca gcgcggtcag ggaaccggtc ttgcccttga cttcaccgtt
     2401 ggtgaacttc tcgacgtact cttcgttgac gcgagctgcg cgctcgagca gacgggagtg
     2461 gatgtagaac acgtcacctg gataagcttc gcggcctggt gggcggcgca gcagcaggga
     2521 gatctgacga taagcgatcg cttgcttggt caagtcatcg taaacgatca gtgcgtcttg
     2581 accacggtca cggaagtatt cgcccatggt gcaaccggca tagggtgcca ggaattgcag
     2641 agcggcggag tcggaagcgg aagcggcaac gacgatggtg tattccagcg cgccgttctc
     2701 ttccagcttg cgtaccacgt tggcgatggt cgaagccttc tggccgatcg caacgtagat
     2761 acaggtcatg ttctgaccct tttgattgat gatggcatcg acagcgacag cggtcttgcc
     2821 agtctgacgg tcgccgatga tcagttcgcg ctgtccgcga ccgaccggca ccatggagtc
     2881 gatggacttc aggccagtct gcactggctg ggaaaccgac ttacgggcga tcacgcctgg
     2941 cgccaccttt tcgatcttgt cggtgatctt ggcattgacc ggacccttgc cgtcaatcgg
     3001 ctggcccagt gcgttgacca cgcggccgat cagctcaggg cctacaggca cttccagaat
     3061 gcggccggta cacttgcagg tatcgccttc ggtgatgtgc tcataggcgc ccagaaccac
     3121 ggcaccgacg gagtcacgct ccaggttcag cgcgaggcca taggtgttgc ccggaaattc
     3181 gatcatttcg ccggccatca cgttggacag gccatggata cgaacgatac cgtcggtcac
     3241 cgagatcacg gtaccttggg tacgtgcttc agcgctcgag gaaaaattct ctaatctgct
     3301 cttaatcagt tcactgattt ctgatgtgga taactgcatt actgtctcct gacctttaag
     3361 gcctaacctt taagcgtata agccaattct tgcaattggc cacggacgga agcgtcaatg
     3421 accgtatcgc cgactaaaat tttgatcccg ccgatgatct ccgggtccac tttgacgctt
     3481 gcttcaatct ttttaccaaa cttgctttcc aggcgtttca ccaacgcctt gacttcggca
     3541 tcgcttggtt tggcagctgc ggtaatctca gcctccagca cgccttcgtc ctgcgccttg
     3601 agttcttcaa aagctgcaac gacctcaggc aacagggcca gacggtcgta ttcaaccaac
     3661 agcttgatca gattcttgcc ggcctcgtcg agcttgtcgc cagcgacggc cagaaacagt
     3721 ttctcctggt cggcactcga caccttgggg tcggcgatat atgcctgcat ctgggcatcg
     3781 gcagcgacgg ccgctgcaaa gcccagcatc tcggaccact tggccagcgc cttctgctct
     3841 ttggcaagac ggaaagccgc gactgcgtaa ggtcttacta tcgttaccgc ttcagccatg
     3901 atcgcttgct tcctatagct cgttagcgat ggttttcagc atatccgcat gtgccttggc
     3961 atcgatctct ttgcgcagga tcttctctgc gccagccacg gccagttcgg agacttgctg
     4021 acgcaggcct tccttggcgc gattgacttc ctgttcgatc tcggccttgg caccggcgat
     4081 gatgcgatca ccttcggtct tggcgttagt cttggattct tcgacgatct cggtcgcgcg
     4141 cttttcagcc tgggcgatga tatcggaagc tttttcctta gctgcatgca gttcctgggt
     4201 cgccttcttg gcggccagct ccagctcgtg cttaccgcga tctgcagcgg cgaggccgtc
     4261 tgcgatctgc ttctggcgtg ctgccagtgc gttcatgatc ggtggccaca cgaacttcat
     4321 gcagaaccag acgaacaccg caaacatgat cgcctgtgca ataagggttg cattgatatt
     4381 catgcaaata cctctcttag tttgctttta tctagggttg ccgacggatt agcctgcaac
     4441 tgcaaacaga acgtacatgg cgataccgac agcgatcatt ggtaccgcgt cgaccagacc
     4501 catgacgatg aagaactggg tacgcagcat cgggatcagt tctggctgac gtgcagcgcc
     4561 ttcgaggaag cggccaccca ggataccgat accgacagcg gcacccagtg cgcccagacc
     4621 cagcatgatc gcgccagcga taaacaacag tgcttgttcc atttgtttct atctcctgta
     4681 aaaaatctac tgctaaaaat tgattgaaat ttagtgatgc tcttgatgcg ccatatccag
     4741 atacacaatg gtcaaggtca tgaagatgaa cgcttgcaga gtgatgatca gaatgtggaa
     4801 gattgcccat accagcgaca gcaggatctg agaactgaac agcgtcgcat tggcaatggt
     4861 gaacccggcc caggtactac ccatgatggc gatcaggatg aagatcattt cgccggcata
     4921 catgttgccg aacaaccgca gggcgaggga gatgggcttg gcgatcaggt tgacgccttc
     4981 aaggaagata ttggccggca ggccgaactt gccgaatggt tgcagcgtca gttcgccgat
     5041 gaacccgccc aagcccttca tcttgacgct gtagaacagc acgatgaaga acaccgagat
     5101 cgccatgccg aaagtggcgt tgggatcagt ggtcggcacg atcttgaaga acatgtgcgg
     5161 atcgatctgg tggatgatga actgaccgaa gaaatcgatc ggcaccagat ccatcaggtt
     5221 catgaggaag atccacatga aaatggtcaa tgccaatggg gcaacgagag aattcttgaa
     5281 actgaaggag ccgcggacgc tggaatcgat gaattcgatg acccattcga cgaaattgac
     5341 cacaccactg ggaacgccgg cgctggcttt tctggctgcg cgggtgaaga agaacaggaa
     5401 cacgacgcca agcagcagcg acatgaggac ggtgtcgagg ttgatcgccc agaagcccat
     5461 ttccttcgcc tcctgcgcac tgtgcgcaca atgaaggtgg ccttcattga tggtacaggt
     5521 cagattctgc aggtggtgct tgatatattc tgaagaggtt aaggtttcgc tagccatttt
     5581 ttacttaaca gtctttattt aacttccaca cgcgatcgat tcaataatcc gcgcaccaaa
     5641 ggcacaagca tcaatatcgc ctgacccatc acaaaaccca ggatcacctg gcctgccggc
     5701 agatgcagac gcaacatgcc aaaagccagc aacccgatta caacaaaaaa ccgttccaaa
     5761 gcggaacggt acatccatag caattgcctg cccgcagtcg cgtcccggtg cttgccctgc
     5821 aacatgcgcc aacttaaaag cgacacgttc accaaggcgc acgcttcgcc ccagatgacg
     5881 gatattcccg actgcggatt atcgatgagg taagttacac cggcgctgat tgttatgaga
     5941 aggatttgca ggccgagcac gatcaagccg tgattcttgc ccgcaatttc tatttttgcg
     6001 ccagacaaca aggacttgga tactagcaat gaatgtaaac gatgtcaaac aaaggccggt
     6061 cttacagggt atccggctat tctctgcaag acacgcccat caggctcaga atgccgtcga
     6121 gctggtcaag atcccggtat tcgatgacga gcttgcctgc tcctttggct ccctgtttga
     6181 tgctgacgcg ggcgccgaca cgatcggcgg cgatgtcttc caggcgcaac aggtctcggt
     6241 caggcttcgg cctggcggtc tttttcaccg gtttttgctc ggcgttggcc agtcgttcgg
     6301 cttcgcgcac ggacaaaccc ttgtgcacga tctgctgggc agccatcacc tgacgcgcag
     6361 gatcgagcgc caatagcgcg cgggcatgac ccatgtcgat ctggttgtgc atgagcatct
     6421 cctgcacagg cgccgccagg ttgagcaggc gcagcaggtt ggtcaccgcg gcacgcgagc
     6481 ggccgacagc ctccgccgcc gcctgatgcg tcatggcgaa ttcgtcgatg aggcgcttga
     6541 taccgttggc ctcttccagc gggttgaggt tctcgcgctg gatgttctcg atcaacgcca
     6601 tcgccagcgc cgaatcgtcc gggatctcgc gcaccagcac cggcacttcc ggcaggccgg
     6661 caagctgcga ggcacgccag cggcgttcac cggcgacgat ctcgaaccga tcatcggcga
     6721 tctcgcggac caggataggc tgcatgatgc cctgcgcctt gatcgaggcc gcaagactgg
     6781 ccagtgactc ctgatccatc tgtgtacgcg gttgatactt gcccggctgc aactggctga
     6841 ctttcagcat acgcaatgaa tctgcctcgc cgacgctgcc gacgtcgcct gccagaaggg
     6901 catcgagacc gcgccccaaa cctttggatt taaccattga ccactccacg tttgatgatt
     6961 tcgtctgcca gcgccaggta agcctgcgca cccttggagg cgcgatcata gatcagcgcc
     7021 ggcatcccgt agctcggggc ttccgccagc cggatatt
//
LOCUS       DATMTW010000304         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_429325, whole genome shotgun sequence.
ACCESSION   DATMTW010000304 DATMTW010000000
VERSION     DATMTW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429325"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VN084_05950"
     CDS             complement(<1..601)
                     /locus_tag="VN084_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HWT29035.1"
                     /translation="MTEFTFVLLIPVVILIWKGIRIVPQGEEWVVERLGKFNAVLGPG
                     LHVINPIFSKISYKVTTKDIILDIPEQEVITRDNAVILANAVAFIKVSNIQRAVYGIE
                     NFQEAMRNMIQTNLRSIIGGMDLNHALTSRDRIKAELKAAIADEAADWGLTVKSVEIQ
                     DIKPSQNMQHAMEQQASAERERVAVVTRAEGEKQSLILNA"
     gene            complement(617..1174)
                     /locus_tag="VN084_05955"
     CDS             complement(617..1174)
                     /locus_tag="VN084_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWT29036.1"
                     /translation="MKCSSSLGRGFSKAKSGKIGTASDKKRPINDTEDDKMLEGTIWS
                     WGVLGLILLAIEMATGTFYILWFGIAALCVALALLILPGLPLSVQLVMFAALSLSSLA
                     IWKLHYKKEGLQLRVGQSQGDEIGRVGVITEAVSARQNGKIAFAQGVMGSREWTAIAD
                     HDLAVGDEAVIVQVEGNALRVQKNT"
     gene            1346..>2049
                     /locus_tag="VN084_05960"
     CDS             1346..>2049
                     /locus_tag="VN084_05960"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWT29037.1"
                     /translation="MHAPIHIVWFKRDLRIEDHLPLAEACRQGPVLPLFAWEPEVIAG
                     EDHARQHQAFAEECLAELEAALAQLGLRLLRWPHGVIDALETIRRQYAVAAIWSHEET
                     GNAITFNIDKRVAAWCRTYGVTWHEFPQHGVVRRLAHRDRWNGHWERRMQAPQTSLPD
                     AITGVTWLQPRDMAPIRASGEDKPSRQHGGRTRAHRILDGFLTTRASHYRPCMSSPIT
                     AGQACSRLSPYLAWGVL"
BASE COUNT          368 a          653 c          613 g          415 t
ORIGIN      
        1 cggcgttgag gatcagagac tgcttctcac cttcggcacg cgtcaccacc gcgacccgct
       61 cgcgttcggc cgaggcttgc tgctccatcg catgttgcat gttctgcgaa ggcttgatgt
      121 cctggatctc gaccgatttc acggtcaggc cccaatccgc ggcttcatcc gcgatggcgg
      181 ctttcagttc ggccttgatg cgatcgcgcg aagtcagggc gtggttaagg tccatgccgc
      241 cgatgatgga gcgcagattg gtctggatca tgttgcgcat cgcttcctgg aaattctcga
      301 tgccgtagac ggcacgctgg atgttgctga ccttgatgaa cgcgaccgcg ttggccagga
      361 tcacggcgtt gtcgcgcgtg atgacctcct gctctgggat gtcgaggatg atgtccttgg
      421 tggtgacctt gtagctgatc ttgctgaaga tcgggttgat gacatgcagg ccaggcccaa
      481 gcacggcgtt gaacttgcct agccgctcca cgacccattc ctcgccttgc ggcacgatgc
      541 ggataccctt ccagataagg atgacgacag gtatcagtaa tacgaaggtg aattcagtca
      601 tgcgggttcc cctgggttaa gtgtttttct gtacgcgcag ggcattgccc tcgacctgca
      661 cgatgacggc ttcgtcgccg acggcgaggt catggtcggc gatcgccgtc cattcgcggc
      721 tgcccatcac gccctgtgca aaggcgatct tgccgttctg gcgagcactg actgcctcgg
      781 tgatgacgcc gacgcggccg atctcatcgc cctgcgactg gcccacgcgc aactgcaatc
      841 cttctttctt gtaatgcagc ttccagatcg ccagcgaact cagcgacaag gcggcgaaca
      901 tcaccagttg cacgctcagc ggcaggcctg gcaggatgag cagggcgagc gcgacgcaga
      961 gggcggcgat gccgaaccac aggatgtaga acgtgcctgt ggccatctcg atcgctagca
     1021 ggatcagccc cagcaccccc caactccata tcgtaccctc gagcatcttg tcgtcctccg
     1081 tgtcgttgat cggtcgcttc ttgtcggatg ccgtgcctat cttaccggac tttgccttgc
     1141 tgaaccctcg gcccagactg ctgctgcact tcacgtatcg cagccgctcc gcggcgatca
     1201 cggcaacatg ctatcagaat tcgccgaggg tggatttctg ccgtctgccg tcgtccgcag
     1261 atcgaacgaa gccaaacgga accgtcagcc ggtgacaggc tccaacaccc aacttccatc
     1321 cagtcctgaa tgccgtcact agccaatgca tgcaccgatc cacatcgtct ggttcaagcg
     1381 cgacctacgc atcgaagacc atctgccctt ggccgaagcc tgcaggcagg gtccggtgct
     1441 gccgctgttc gcctgggagc ctgaggtcat cgccggcgag gatcacgcac gccagcacca
     1501 ggcgttcgcc gaggaatgcc tcgcagagct cgaagccgca ctggcgcaac tcggtttgcg
     1561 cctgttgcgc tggccgcatg gcgtcatcga cgcgctggag acgatccgcc ggcaatatgc
     1621 ggtggcggcg atatggagtc atgaagagac tggcaatgcg atcaccttta acatcgacaa
     1681 gcgcgttgcg gcctggtgtc gtacctacgg cgtgacatgg cacgaattcc cgcagcatgg
     1741 cgtggtccgg cggttggcgc atcgcgatcg ctggaacggt cactgggaac gtcgcatgca
     1801 ggcgccgcag acatcgctgc cggatgccat cacaggcgtc acctggctgc agcctcgcga
     1861 tatggcgcct atccgcgcta gcggcgagga caagccatct cggcaacacg gtggccgcac
     1921 acgcgcacac aggatcctcg acggcttcct gacgacgcgc gccagccatt atcggccctg
     1981 catgtcgagc ccgatcacgg ctggccaggc ctgctcgcga ctgtcgccgt atctcgcctg
     2041 gggtgtgct
//
LOCUS       DATMTW010000305         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_159629, whole genome shotgun sequence.
ACCESSION   DATMTW010000305 DATMTW010000000
VERSION     DATMTW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159629"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VN084_05965"
     CDS             <1..626
                     /locus_tag="VN084_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWT29038.1"
                     /translation="DWFSALKNTLRQAPDVILIGEIRDRETMEFALAFAETGHLCMAT
                     LHANSANQALDRIVNFFPEERHAQLHMDLSLNLRAFVSQRLVPKKGGGRCAAIEILLN
                     SPLVADLILKGETNMVKEAMAKSVELGMQTFDQALFQLCEEGRITEEDALRNADSINE
                     LRLRFKLHSKSASSKDAGSRIDHLSLHEDEPEVEEEEAAQPSAVTPA"
     gene            626..940
                     /locus_tag="VN084_05970"
     CDS             626..940
                     /locus_tag="VN084_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HWT29039.1"
                     /translation="MMEITLLILCLLAGWFWLDTIGARETAIARGRDLAERCNLQLLD
                     ETVACDKLRLGRNSRGHLQLMRIYEFEVSADGSNRMPCQLVLLGRELQSWYIPPYLQP
                     LH"
     gene            1036..1929
                     /gene="gluQRS"
                     /locus_tag="VN084_05975"
     CDS             1036..1929
                     /gene="gluQRS"
                     /locus_tag="VN084_05975"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWT29040.1"
                     /translation="MYIGRFAPSPTGPLHFGSLVAAVASYLEAKRAGGKWLVRMEDLD
                     GPREMPGAADDILRTLQAFGLHWDDSVVYQSQRLAHYAEALDRLQQRGLVYPCACSRK
                     EIADSAMSGIDGLVYPGTCRDGLPAGRQPRAWRIRVEDTEIGFRDRIQGLLSQNLARD
                     IGDFVLKRADGFFAYQLAVVVDDGLQGVTDIVRGADLLDSTPRQIHLQQLLGLPPTSY
                     THVPVAANQRGEKLSKQTLARPLDPHQPQQQLWQALDYLRQNPWPELRQASLGTLWSW
                     ALTHWQVSRIPSQRSITTNLE"
     gene            complement(1959..>2959)
                     /locus_tag="VN084_05980"
     CDS             complement(1959..>2959)
                     /locus_tag="VN084_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWT29041.1"
                     /translation="VSVHNLSQMHEMTSLLQSCSDIAESLEVIQQFASRLFPAGGGVL
                     YLFRESRNQLSAALSWGAPAHSKEIFQPEDCWALRRGEPHKLDHTRHSLACSHMQHAE
                     DIVSLCIPVIAKGNVLGILYLQRLPDNPLNQVEYRLAETMSSQIALALSNIKLRDTLR
                     DLSVRDPLTGLFNRRYMEESLQREIATAKRKERQLGLAMLDVDHFKKFNDTFGHDAGD
                     LLLREMGQLLVQRSRAGDIACRFGGEEFVMIYPEASPEIVIKLAEELRQVIYDLQIQH
                     FGRSLGQTTASIGLAFFPQHGNTTDALLRAADKALYSAKGAGRNCVQVAESPPDWS"
BASE COUNT          600 a          941 c          856 g          562 t
ORIGIN      
        1 acgactggtt ctcggcactg aagaacaccc tgcgccaggc gccggatgtc atcctcatcg
       61 gcgagatccg cgaccgcgag accatggaat tcgcgctggc tttcgccgag accggccact
      121 tgtgcatggc gacgctgcac gccaacagtg ccaaccaggc gctggatcgt atcgtcaact
      181 tctttcccga agagcgtcat gcgcaattgc acatggacct ctcactcaac ctgcgcgcct
      241 tcgtctccca gcggctggta ccaaaaaagg gaggcggccg ctgcgccgcc atcgagatcc
      301 tgctcaattc accgctggtc gccgatctta tcctcaaggg cgagaccaac atggtcaagg
      361 aagccatggc aaaatccgtc gagctcggca tgcagacttt cgaccaggcc ctgttccagc
      421 tctgcgaaga aggccgtatc accgaagaag acgcattgcg taacgccgat tcgatcaacg
      481 aattgcgcct gcgtttcaag ctgcacagca agagcgcttc gtccaaggat gccggctcac
      541 gtatcgacca tctgtccttg catgaagatg agcctgaggt agaagaggaa gaagctgcgc
      601 aaccatcagc tgtaacgccg gcctgatgat ggagattact ctgctcatcc tctgcttgct
      661 ggccggctgg ttctggctcg acaccatcgg cgcgcgcgag acggccatcg cccgcggccg
      721 ggatcttgcc gaacgctgca acctgcaatt gctggacgag accgtggcct gcgacaagct
      781 tcgcctggga cgtaacagtc gcggccatct gcagctcatg cgcatctacg aattcgaggt
      841 cagcgccgac ggcagcaacc gcatgccctg ccaactggtg ctgctgggtc gggaactgca
      901 aagttggtac atcccaccct acctgcaacc cttgcactga aagccagcca tcatgaccgt
      961 gcggattgaa aggcccctgg cgatacgcac gcccgatgtg atggcggcgc acctgtcctg
     1021 ctcaggccat taggcatgta tatcggtcga tttgcgccat cgcccaccgg tccattacat
     1081 ttcggctcgc tggtcgctgc cgtcgccagc tatctggaag cgaaacgcgc cggcggcaaa
     1141 tggctggtac gcatggaaga cctcgacggc ccgcgagaga tgccgggcgc cgcggacgat
     1201 atcctgcgca ccctgcaggc attcggcctg cactgggacg acagcgtcgt ctaccagagc
     1261 cagcggctcg cgcactatgc cgaagcgctc gatcgcctgc agcagcgggg cctggtctac
     1321 ccttgcgcct gctcacgcaa ggagatcgcc gactcggcga tgagcggcat cgatgggctg
     1381 gtctatcccg gcacctgccg cgatggcctg cctgcaggcc gccagcctcg tgcctggcgc
     1441 attcgcgtcg aggataccga gatcggcttc cgcgaccgca tccagggcct gctaagtcag
     1501 aatcttgcgc gggatatcgg cgactttgtg cttaagcgtg ccgatggttt ctttgcctat
     1561 cagctggcgg tcgtcgtcga tgacgggctc cagggcgtga ccgacatcgt gcgtggggcc
     1621 gacctgctgg attccacacc acggcagatc catcttcagc agctactcgg cctgccgccc
     1681 accagctaca cccatgtgcc ggtcgccgcc aaccagcgtg gcgaaaagct cagcaagcag
     1741 acgctggcca ggccactcga cccgcaccag ccacagcagc aactctggca ggcactggat
     1801 tatctgcgtc agaacccctg gcctgaactc aggcaagcca gtctcggcac gctctggtca
     1861 tgggcgctga cacactggca agtctcgcgc atccccagcc agagaagcat cacgacaaac
     1921 ctggagtgag gccgaccggc caagacaatc ccgcgggcct aagaccagtc aggcggtgat
     1981 tcggccactt gtacacaatt tcgcccggcc cctttggctg agtacagcgc tttgtcagca
     2041 gccctcagca aggcgtcggt ggtgttgccg tgctgtggga agaacgccaa gcctatcgat
     2101 gccgtcgtct gccccagcga gcgcccgaaa tgctggatct gcaagtcgta tatcacttgt
     2161 cgaagttctt cagccagctt gatcacgatc tccggagagg cctccgggta gatcatgacg
     2221 aactcctcac cgccgaagcg gcatgcgata tctccggcac gagaacgctg caccaacaac
     2281 tgccccatct cacgtaacag caagtcgcca gcgtcatggc cgaaggtatc gttgaatttc
     2341 ttgaaatgat cgacatccag catggcaagc cctagctgac gctccttgcg tttggcggtc
     2401 gcgatctccc gttgcaacga ctcttccatg tagcggcgat tgaacagccc cgtcaatggg
     2461 tcacgcactg acaggtcacg caaggtatct cgcaacttga tattggagag cgccagtgcg
     2521 atctggctcg acatcgtctc ggccagccgg tactcgactt ggttaagagg attgtccggc
     2581 agcctttgca gatacagaat gcccagcaca ttgcccttgg cgatcacagg tatgcaaagg
     2641 ctaacgatat cctcggcgtg ctgcatgtga ctgcaggcga gcgagtgacg ggtgtgatcg
     2701 agtttgtgtg gttcgcctcg ccgaagtgcc cagcagtcct cgggctggaa tatttccttg
     2761 ctgtgggcgg gcgctcccca gctcagcgct gccgataact ggttgcgtga ttcacggaac
     2821 agatagagca cgccaccccc tgccgggaag aggcgcgacg cgaactgctg gatgacctcc
     2881 agggactcgg caatatcgct gcaggattgc aacaagctgg tcatttcgtg catctgcgag
     2941 aggttatgga cggacacat
//
LOCUS       DATMTW010000306         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_429551, whole genome shotgun sequence.
ACCESSION   DATMTW010000306 DATMTW010000000
VERSION     DATMTW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429551"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VN084_05985"
     CDS             complement(<1..309)
                     /locus_tag="VN084_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWT29042.1"
                     /translation="MPTMHMPTLHTKIGIACLVLLLALSGCSKPEDNAKKGPAATLIS
                     TAVAQAAALEVREQTVGTLEGFIDPTIAAEVAARVVQISATPGRAVKKGELLAVLDVT
                     D"
     gene            complement(342..884)
                     /gene="ampD"
                     /locus_tag="VN084_05990"
     CDS             complement(342..884)
                     /gene="ampD"
                     /locus_tag="VN084_05990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HWT29043.1"
                     /translation="MNVPLEINAAGIAHEALWVDSPNRDDRPVAEEIVLAVIHNISLP
                     PGEYGGEGIIELFTNRLDPTAHPYYATIHQLRVSAHFLIRRDGQLVQFVPCGERAWHA
                     GVSSWQGRERCNDFSVGIELEGSDEDAFEAAQYLTLNRLLAGLKQRYPIRDVVSHSEI
                     APGRKTDPGPYFDWSQVARP"
     gene            978..>1828
                     /locus_tag="VN084_05995"
     CDS             978..>1828
                     /locus_tag="VN084_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HWT29044.1"
                     /translation="MVFLTLFLMLIVILIAAEVFTNALEHLGEKLKISEGVTGSLFAA
                     VGTALPETSVPLLAIFAGTSDPHINDEIGVGAILGAPLMLATLSICLMALSVLARRGR
                     HGHLRPERNGLIRDLNFFIMAFALATLAMFIPHEVRAMRIGLSFGMVLIYFIYIMMTI
                     RASKALVSEGHGTEADEPMFLCRVGLPNNMPVIFLQLALGLVLLLAGAKGFINGVEEA
                     AEIIGISTLLLSLLIIPIATELPEKVNSILWIRKGKDTLAFGNITGAMVFQGTLLPAI
                     GILLTPWA"
BASE COUNT          307 a          548 c          562 g          411 t
ORIGIN      
        1 gtcggtgacg tcgagcacag ccagcagctc gcctttcttc actgccctgc cgggcgtggc
       61 cgagatctgc accacccgcg ccgcgacttc ggcggcgatc gtcgggtcga tgaaaccttc
      121 cagcgtgccg accgtctgtt cgcgaacttc cagcgcggca gcctgcgcca cggctgtgct
      181 gatcagtgtc gccgccgggc cttttttggc gttatcttcc ggcttgctgc agccgctgag
      241 tgccagcagc aggacaaggc aggcgatccc gattttcgta tgcagcgttg gcatgtgcat
      301 tgtgggcatg ctgggctttc tataattgtc ttgtttggcg ctcagggccg ggcgacctgt
      361 gaccagtcga aatacgggcc gggatcagtt tttcggcccg gtgcgatctc tgaatggctg
      421 accacatcgc ggatcgggta acgttgcttc agccctgcca gcaaacggtt cagggtcagg
      481 tactgcgcag cttcgaaagc gtcttcgtcg cttccctcca gctcgattcc gaccgagaaa
      541 tcgttgcagc gttcacggcc ctgccagctg gagacgccgg catgccatgc gcgctcgcca
      601 caggggacga actgtacaag ctgaccatct cggcggatga ggaaatgggc agacacgcgc
      661 aactggtgga tggtggcgta ataggggtgt gcggtcgggt cgaggcgatt ggtgaagagc
      721 tcgatgatgc cttcgccgcc atattcgccg ggcggcaggc tgatgttatg gatgacggcg
      781 aggacgatct cctctgcgac gggccggtca tcccgattgg gcgaatcgac ccacagcgct
      841 tcgtgcgcga tgcctgcggc gtttatttcc aacgggacgt tcattttctg cgggtccatt
      901 atttccagcg gggccatgca gctgtccgcg ttatccgata gaatccgtgt tcaacccgca
      961 tcatcaggtc attccccatg gtttttctga cgcttttcct catgctgatc gtgattctaa
     1021 tcgcagcgga ggtgtttacc aacgcacttg agcacctggg cgagaagctc aagatctcgg
     1081 aaggcgtcac cggctcgctg ttcgctgccg tgggtacggc actgccggag acttcggtgc
     1141 ccttgctggc gatctttgcc ggtaccagcg acccgcatat caatgacgag atcggcgtcg
     1201 gcgccattct cggcgcgccg ctgatgctgg cgacactgtc gatctgcctc atggcgctat
     1261 ccgtgctggc gcgtcgtggg cgtcatggcc atctgcgccc ggagcgcaac gggctcatcc
     1321 gcgaccttaa tttcttcatc atggccttcg cgctagccac cctggccatg ttcatcccgc
     1381 atgaggtgcg agccatgcgc atcggcttga gcttcggcat ggtgttgatc tatttcatct
     1441 acatcatgat gaccattcgc gcctcgaaag cgctggtgag cgagggccac ggcacagaag
     1501 ccgacgagcc catgttcctg tgccgcgtcg gcctgccgaa caacatgcct gtgatcttcc
     1561 tgcaactggc gttaggtctg gtgcttctgc tggcaggagc gaagggcttc atcaacggtg
     1621 tggaggaggc ggccgagatt atcggcatct ccaccttgct gctgtcgctc ttgatcattc
     1681 cgatcgccac cgagctgccg gagaaagtga acagtatcct gtggatacgc aaaggcaagg
     1741 acacgctggc cttcggcaac atcaccggcg ccatggtgtt ccagggtacc ttgctgcctg
     1801 cgatcggtat cctgctgacg ccgtgggc
//
LOCUS       DATMTW010000307        10051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_294396, whole genome shotgun sequence.
ACCESSION   DATMTW010000307 DATMTW010000000
VERSION     DATMTW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294396"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2748
                     /gene="purL"
                     /locus_tag="VN084_06000"
                     /gene_synonym="purI"
     CDS             <1..2748
                     /gene="purL"
                     /locus_tag="VN084_06000"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HWT29045.1"
                     /translation="EFGRPNIAGYFRTLELESAGEVRGYHKPIMLAGGVGNISAKHSK
                     KNPIPPGAALIQLGGPAMLIGLGGGAASSMDTGANTENLDFDSVQRGNPELERRAQEV
                     IDRCWQLGDDNPILSIHDVGAGGISNAFPELVNDAGVGALFRLRNVHNEEPGMSPREL
                     WSNEAQERYVMAVAQDDLPRFAEICERERCPFAVVGHATEARHLTVEDSHFDNKPVDM
                     ELSVLLGKPPKMTRDVQHIERRQASFDTSHIDLKEAAQRVLRLPGVADKTFLITIGDR
                     TVTGLIARDQMVGPWQVPVADVAVTLAGYETYRGEAFAIGEKAPLALIDAPASGRMAI
                     GEAITNIAASQIENLGDLKLSANWMAPAGHPGEDAALFDTVHAVGMELCPQLGISIPV
                     GKDSMSMKTVWEEGGEKKAVTAPISLVVTAFAPTPDARKTLTPQLRTDRGDTKLILID
                     LGHGKNRMGGSALAQVYGEVGDLAPDVDDAARLKMFFAAIQNLNSDGKLLAYHDRSDG
                     GLFVTLVEMAFAGRCGLDIELSRLAADTASVLFNEELGAVIQVAADEATAIWTALDAV
                     MPGCIHIIGTAVANNHGIVIRKHGEIVFAEMRANLHRLWSETTYQMQKLRDNPLCAQQ
                     EYDRLLDMGDPGIHHHLTFDLNARVEAPYISTGAKPTMAILREQGVNGQVEMAAAFDR
                     AGFQSHDVHMSDIISGRVSLKDFAGVVACGGFSYGDVLGAGEGWAKSILFNSRARDEF
                     SAFFQRSDAFALGVCNGCQMMSNLHSIIPGAEHWPHFVRNKSEQFEARFAMVEVLDSP
                     SLFFGGMAGSRMPIAVAHGEGYAEFSSATAVNEVLADKLVSMRFVDNSGKATETYPFN
                     PNGSPQGITGLTTRDGRFTIMMPHPERVFRSVQHSWHPDGWGEDGPWLRMFRNARKYV
                     G"
     gene            2789..3274
                     /locus_tag="VN084_06005"
     CDS             2789..3274
                     /locus_tag="VN084_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWT29046.1"
                     /translation="MKIIKVLLVAVAMMTTLSAWALTDDEQVEFMDAVTTGKVAVAKK
                     FIESGTVNVNQTFFAWSPLLSAASKGQLAVVKMLVEHGANMNYQHPVTKMTPLAHAAY
                     EGNDAIVKYLLEKGADPNIKMRGGVSLVRAARDEGHDETVKLLEQFGAKDDGCKDEKC
                     L"
     gene            3343..3657
                     /locus_tag="VN084_06010"
     CDS             3343..3657
                     /locus_tag="VN084_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWT29047.1"
                     /translation="MLSISMMSLGSAQAADDAIHIRTLAASCAACHGTNGNSVGGTPV
                     LAGLDQGHFVSQMMAFRSGARASTVMHHHAKGLTPEEIQQLAAYFAHQKRVTTSTPTT
                     VE"
     gene            3666..4928
                     /locus_tag="VN084_06015"
     CDS             3666..4928
                     /locus_tag="VN084_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWT29048.1"
                     /translation="MAINRRDFLKLTASATLISSPLRVFAKDKPLGRVVVIGGGYAGA
                     TAAKYIRMWSDGAIEVVVIEPNQQFISCPLSNLVLGGSKHIGELMFSYDQLKHNHGIQ
                     WVQDEVTAIDPQARKVRMLRGELSYDKLIVAPGVDFIYDQLPMLQSLEAQILVPHAWK
                     AGPQTVNLHKQLKSMPENGVFTISIPKAPYRCPPGPYERISQVAFYLKQHKPRAKIIA
                     LDANPEITSKKGLFTKAWADLYPGMIDYRPNSSVVDVELSSRTVKTEFETVQSDVLNV
                     IPPQRAGKPAQMAGAVNIDKRWCEVDFVTYESKVVPGVHILGDAVSAALPKSAHIATS
                     QARVCASAIVAQMQGQPVDAAPVFANTCYSYVSDKLAMHVAHVYRYDPAKHAMVAADG
                     GGLSEHPSEQEGDYAQYWAKNIWSDVLT"
     gene            5119..6183
                     /locus_tag="VN084_06020"
     CDS             5119..6183
                     /locus_tag="VN084_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWT29049.1"
                     /translation="MVYADLNQQGASYFSGPRFMAFDAQGQLYLSLGMQNKVVMLPDR
                     DRDGRADEVVLVADKLSAPQGLAFVDGALLVANQDGVVRLEDDAGRWPARKVTPWIKD
                     LPSGGHTLKSLKLGADGYLYLNVGSSCNVCVESDPLRATILRYTKTGQPAGALVTLGR
                     HAQSPVWARGLRNAQGFDWHLSGAMYATNDGSDMRVASKGGKVDDTLPPEHLNRIEGG
                     QHYGWPHCWGQRETDPAFPGSEGFCAATQGPAATFPAHSTPIGIAFLDKSAFPQSYRN
                     SAIVALHGSWNRQEPSGYKLVQVRFEGDQPVAVTDFMTGWLSQSGAWGRPVDVVVGPD
                     GALYVSDDRAGLVYRVTYKP"
     gene            6255..6863
                     /locus_tag="VN084_06025"
     CDS             6255..6863
                     /locus_tag="VN084_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29050.1"
                     /translation="MQRDLIKSIIGGVGGLALMCLLTGAAQSKEADAPAMLVFIHPQD
                     YSSEVSLRHYYYNYWVTQGPQVESAAKEVLGQAFGDVGMCQGNNPGKTLVWLRPSIFY
                     NPLMKQYHGKIRASVYSGSGKPLATYIGEGQADGYLDMRLDRERKIATVYKLAMAKVA
                     EKMKADAALQQELKTNVAPDNAPSPCAMVTLLPAPAVSVMSF"
     gene            6872..7744
                     /locus_tag="VN084_06030"
     CDS             6872..7744
                     /locus_tag="VN084_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWT29051.1"
                     /translation="MSLNKKHIVTLCLGGLLVSIGLSVQAHQGKTVTGLKTPESVVEA
                     PDGRVFVSEIGEFGKDGDGEISYIDKDGKRHPFASGMDDPKGLALIGDDLYVADKTRV
                     LKVGSDGKWQVFAAATAFPTTPKFLNDLEADPHGMLYVSDSGNLEGVGGAIYSIDRDG
                     KVTLLIDGAKDKRVLAPNGLLADDTGKALLEVDFVSGILYKLDLATGEMQELASGFGG
                     GDGLVHHPKGLMFVSDWKNGKVFSVDAVKDEVKLLKAGFKASADIAMSRDGRYVIVPD
                     MKAGEVVWLPIHIK"
     gene            complement(7882..8748)
                     /gene="serB"
                     /locus_tag="VN084_06035"
     CDS             complement(7882..8748)
                     /gene="serB"
                     /locus_tag="VN084_06035"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HWT29052.1"
                     /translation="MTWNPMRFVIQHPAMLPQHLQALSAIAQAHAFTRINADAYYLKF
                     DSEPQAPLLLRLRAYCAEHGLDCALVPDSQTLQHFGLAVMDMDSTLISIECIDEIADM
                     CGLKKEISMVTESAMRGEIEFAESLRQRVALLKGLEESALQRVIDERLTLNPGALQWI
                     QACKQHGITTLLVSGGFDFFAERVRAMTGIDHIRANTLEIVDHRLTGKVLGHIIDGQA
                     KADSLIGLRDQLGLQASQVIAIGDGANDLKMMSVAAVGVAYHAKPIVQQQATYALNQM
                     GLDGIANLFTVI"
     gene            8881..10050
                     /gene="sucC"
                     /locus_tag="VN084_06040"
     CDS             8881..10050
                     /gene="sucC"
                     /locus_tag="VN084_06040"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWT29053.1"
                     /translation="MNLHEFQAKHLLQHYGLAIPKGEVVDTADAATLAAKQIGGDAWV
                     VKAQIHAGGRGKSGGVKLVRSNAELRTAVESLIGKPLVTYQNAPDGQLVTRVLVEQTL
                     PIARELYLSLLVDRSLERVVMVASASGGMDIEEIAAHHPEQILQEVCDPLNGLVDFQA
                     RNLAFGLGLVGDQVAAFTRLAKGLYRLFKENDLSLLEINPLVVTTDGALVALDCKISI
                     DDNALYRQKALAEQRDWSQDDAKEAQAHSSGLNYIALNGNIGCMVNGAGLAMATMDII
                     KLYGAEPANFLDVGGGASKEKVTAAFKIITADPNVKGILVNIFGGIMRCDIIAEGVVA
                     AVKEVGLKVPLVVRLEGTNVTEGKAIINNSGLNVIAANDLADAAEKVVKAVRSAA"
BASE COUNT         2013 a         2997 c         3133 g         1908 t
ORIGIN      
        1 gaattcggcc ggcccaacat cgccggctat ttccgcacgc tggagcttga gtccgcgggc
       61 gaagtgcgcg gttaccacaa gcccatcatg ctggcgggcg gcgtgggcaa tatctccgcc
      121 aagcactcga agaagaaccc gattccgcct ggcgcggcgc tcattcaatt gggcggcccg
      181 gcgatgctga tcggcctcgg cggcggcgcg gcgtccagca tggataccgg cgccaatacc
      241 gagaaccttg atttcgattc cgtacagcgc ggcaaccccg agctggaacg ccgcgcgcag
      301 gaagtcatcg accgctgctg gcaactgggc gacgataatc ccatcctcag catccacgat
      361 gtcggcgcag gcggcatctc caatgccttc cccgagctgg tcaacgatgc cggtgtcggc
      421 gccttgttcc ggctgcgcaa cgtacacaac gaagagccgg gcatgagccc gcgcgagttg
      481 tggagtaacg aggcgcagga gcgttatgtc atggctgtcg cgcaggatga cctgccgcgc
      541 ttcgccgaga tctgtgaacg cgagcgctgt ccgttcgccg tcgtcggcca tgccactgag
      601 gcgcggcacc tgacggtgga agacagccat ttcgacaaca agccagtcga tatggagctt
      661 tcggtactgc ttggcaagcc accgaagatg acgcgtgacg tgcagcatat cgaacgtcgc
      721 caggcgagct tcgataccag ccacatcgac ctcaaggagg ccgcgcaacg tgtgctgcgc
      781 ttgccgggcg tcgccgacaa gaccttcctc atcaccatcg gtgaccgtac cgtcaccggc
      841 ctcatcgccc gcgaccagat ggtcggcccg tggcaagtgc cggtggccga tgtcgccgtg
      901 acgctggcgg gctacgagac ctatcgcggc gaagccttcg ccatcggcga aaaagccccg
      961 ctggccttga tcgacgcgcc agcttccggt cgcatggcga tcggcgaggc catcaccaat
     1021 atcgccgcga gccagatcga gaacctgggc gacctcaagc tttccgccaa ctggatggcg
     1081 ccggccggcc atccgggcga agacgccgcc ctgttcgata ccgtgcacgc ggtcggcatg
     1141 gagctgtgtc cgcagctcgg catcagcatc ccggtgggca aggattcgat gtcgatgaag
     1201 acggtgtggg aagaaggcgg cgagaagaag gccgtgaccg cgccgatctc gctggtggtg
     1261 acggcgttcg cgccgacgcc tgacgcccgc aagacgctga cgccgcaact gcgcaccgac
     1321 cgtggcgaca ccaagctgat cctcatcgac ctcggtcatg gcaagaaccg catgggcgga
     1381 tcggccttgg cgcaggtcta cggcgaggtc ggcgacctcg cgccggacgt cgacgatgcc
     1441 gcgcgcttga agatgttctt cgccgccatc cagaacctca atagtgacgg caagctgctg
     1501 gcttaccatg atcgttccga cggcggcctc ttcgtcaccc tggtggaaat ggcgttcgcc
     1561 ggccgttgcg gccttgatat cgaattgagc cgcctcgccg cggacacggc cagtgtgctg
     1621 tttaacgaag agctgggcgc agtgatccag gtcgcagccg atgaagcgac cgccatctgg
     1681 accgcacttg atgccgtgat gcccggttgc atccacatca tcggcactgc ggtcgccaac
     1741 aaccatggca tcgtcatccg caagcatggc gagatcgtct tcgccgagat gcgcgccaac
     1801 ctgcaccgcc tgtggtccga gaccacctac cagatgcaga agctgcgcga taacccgctg
     1861 tgcgcgcagc aggaatatga tcgtctgctg gacatgggcg accctggcat ccatcaccac
     1921 ctgacattcg acctcaatgc gcgtgtcgaa gcgccctaca tcagcaccgg cgcgaagccg
     1981 acgatggcga tcctgcgcga gcagggcgtc aacggccagg tcgagatggc ggcggccttc
     2041 gaccgtgccg gtttccagag ccatgacgtg cacatgagcg acatcatcag tgggcgcgtc
     2101 agcctgaaag acttcgccgg tgtcgtggct tgtggcggct tctcctatgg tgacgtactc
     2161 ggtgccggcg agggctgggc caagtccatc ctgttcaaca gccgtgcccg tgacgagttc
     2221 tcggccttct tccagcgcag cgatgccttt gcgcttggcg tctgcaacgg ctgccagatg
     2281 atgagcaacc tgcacagcat catcccgggc gctgaacact ggccgcattt cgtacgcaac
     2341 aagtcggagc agttcgaggc gcgattcgcc atggtcgagg tgctggactc gccatcgctg
     2401 ttcttcggcg gcatggcggg tagccgtatg ccgatcgctg tcgcgcatgg tgaaggttat
     2461 gcggaatttt cttcggcaac cgctgtcaat gaggtgcttg cagacaaact ggtcagtatg
     2521 cgctttgtgg acaactcggg gaaagcgacg gagacctatc cattcaaccc caacggctcc
     2581 ccgcagggga tcacgggtct gacgacacgt gacgggcgat tcacgatcat gatgccgcat
     2641 ccggaacgcg ttttccgtag cgtgcagcac tcgtggcacc cggatggctg gggcgaggat
     2701 ggaccgtggc tgaggatgtt ccgcaatgca aggaagtatg tagggtaatt acatttattt
     2761 aacgataatc agcaggagat agatcagcat gaaaataatc aaagtgttac tggtcgcagt
     2821 tgcaatgatg accaccttgt ccgcctgggc gttgaccgac gacgaacagg tcgaattcat
     2881 ggacgccgtg accaccggta aggtcgctgt cgccaagaaa ttcatcgaat ccggcacggt
     2941 caatgtcaat caaaccttct tcgcctggtc tccgctgctg tctgctgcga gcaaggggca
     3001 gctggctgta gtcaagatgc tggtcgaaca tggtgccaac atgaactacc aacatcccgt
     3061 gaccaagatg accccgctgg cacacgctgc ctacgaaggt aatgacgcca tcgtgaagta
     3121 cctgctggaa aagggtgcgg accctaacat caagatgcgt ggcggtgttt ccctggtacg
     3181 tgccgcacgt gacgaaggcc atgacgaaac cgtcaagctg ctcgagcagt tcggcgccaa
     3241 ggacgatggc tgcaaagatg agaagtgcct gtaactatgc ttaaatagca tcatccgacc
     3301 acgcagggga gccgatatat gcagaagcgt cacttggcat ggatgctgag tatctcgatg
     3361 atgtccctgg gttcggcgca agccgcggat gatgccatcc atatccggac actggctgcc
     3421 tcctgcgcag cctgtcacgg aaccaacggc aacagcgtag gcggcacgcc agtgcttgcc
     3481 ggcctcgatc aggggcattt tgtcagtcag atgatggcgt tccgttccgg cgcgcgagct
     3541 tccacggtca tgcatcacca cgccaaaggc ctcacgcctg aggagatcca gcagcttgct
     3601 gcctacttcg cgcatcaaaa gcgtgtcacg accagcaccc ccacgactgt ggaataggag
     3661 tcgacatggc catcaaccga cgtgatttcc tcaagctgac cgccagcgcg accctgatct
     3721 ccagtccgct tcgggtattc gcaaaagaca agccgctggg ccgcgtcgtc gtgatcggcg
     3781 gcggctacgc cggggcgacc gctgcgaaat atatccgcat gtggagcgac ggcgcgatcg
     3841 aagtcgtcgt catcgagccc aatcagcagt tcatctcctg tccgctcagc aatctcgtcc
     3901 ttggcggcag caagcacatc ggcgaactga tgttcagtta tgaccagctc aagcacaacc
     3961 acggtatcca gtgggtgcag gacgaagtca cggcgatcga tccgcaggcg cgcaaggtgc
     4021 gcatgctgcg cggcgagctc agctatgaca agctgatcgt cgctcccggc gtcgatttca
     4081 tctacgacca gctccctatg ctgcaaagcc tggaggcaca gatacttgtg ccacacgcct
     4141 ggaaagcggg gccgcagacc gtcaacctgc acaagcagtt gaagtcgatg ccggagaacg
     4201 gggtgttcac catcagcatc cccaaggcgc cttaccgctg cccgccaggc ccgtatgagc
     4261 ggatcagcca ggtcgctttc tacctcaagc aacacaagcc cagggccaag atcatcgccc
     4321 ttgatgccaa tcccgagatc acctcgaaga aggggttatt caccaaagca tgggccgacc
     4381 tttatccggg catgatcgat taccgcccga acagcagcgt ggtcgatgtc gaattgtctt
     4441 cgcgtaccgt caagaccgag ttcgagaccg tgcaatccga cgtccttaac gtgatcccgc
     4501 cgcagcgtgc gggcaaaccc gcgcagatgg ccggcgcggt caatatcgac aagcgctggt
     4561 gcgaggtcga tttcgtcact tatgagtcga aggtggtgcc tggcgtgcat atccttggtg
     4621 atgcagtctc cgctgccctg cccaagtccg cacatatcgc gacctcgcaa gcccgagtct
     4681 gcgccagcgc gatcgtggcg cagatgcagg gccagcccgt tgacgcagca cctgtgttcg
     4741 ccaatacctg ctacagctat gtcagcgaca agctggcgat gcatgtcgcc catgtctatc
     4801 gctatgatcc tgccaagcat gcgatggtcg cggctgatgg cggtggcctg tcggaacatc
     4861 cttccgagca ggaaggcgac tatgcgcaat actgggcgaa gaacatctgg tcggatgtcc
     4921 ttacctgaag aaggctgcgc cttgcgttat tcgatttccg catcccgttt ggtgagtcat
     4981 ctcaggggta ttttgacttg cgttgtggcg cttgcgcttt gcgccatcgc gctcccaggt
     5041 caggccgagc cagcagagac gttgcctttg ccgcaagacc cggcagcggc gctcaacttg
     5101 ccggcgggct ttcatgcaat ggtctacgct gacctcaacc agcagggcgc gtcctatttc
     5161 agcggcccgc gcttcatggc gttcgatgcc caggggcagc tctacctctc gctcggcatg
     5221 cagaacaagg tcgtgatgct gccggaccgc gaccgtgatg gtcgcgctga cgaggtcgtg
     5281 ctcgtcgccg acaagctcag cgcaccgcag gggctagcct tcgttgatgg cgcgctgctg
     5341 gtggcgaatc aggatggggt cgtgcgcctg gaagacgatg cgggccggtg gccggcgcgc
     5401 aaggtcacac catggatcaa agacctgccg agcggcgggc acaccctgaa atccctgaag
     5461 ctgggcgctg acggctatct ctacctgaat gtcggctcaa gctgtaacgt ctgtgtcgag
     5521 tctgacccgc tgcgtgcgac catcttgcga tataccaaaa ccgggcagcc cgccggtgcc
     5581 ctggtgactc tggggcgcca tgcccagtcg cctgtctggg cgaggggcct gcgcaatgcg
     5641 caagggttcg attggcatct gtccggtgcc atgtatgcca cgaacgacgg ttcggacatg
     5701 cgcgtggcaa gcaaaggcgg caaggtagac gataccctgc cgccggaaca cctgaaccgg
     5761 atcgagggcg ggcaacatta tggctggccg cattgctggg gccagcgcga gaccgacccc
     5821 gcttttcccg gcagtgaggg attctgcgcc gctacccagg gtccggcagc gaccttcccg
     5881 gcgcattcga ccccgatcgg catcgctttt ttggacaaat cggcattccc gcagtcatac
     5941 cgtaacagcg cgatcgtcgc cttgcatggc tcgtggaacc ggcaggagcc ttccggttac
     6001 aagctggtgc aggtcaggtt cgaaggcgat cagccggtcg cggtcacgga tttcatgacc
     6061 ggctggttga gtcagtccgg agcctggggc agaccagtgg atgtcgtggt cggcccggat
     6121 ggcgcactgt atgtgtcgga tgaccgggca ggcctggtct accgtgtgac ctacaagcct
     6181 tgaggcttgt ccgaacatga ggcctgtgtg aacgacaggc tcgtcagaat agccatatga
     6241 aaggatgtaa catcatgcag cgcgatctca ttaaaagcat catcggcgga gtcggcggac
     6301 tggccctgat gtgcctgctt acgggtgcgg cccagtccaa ggaagctgat gccccggcga
     6361 tgctggtgtt catccatccg caggattact cgagcgaagt cagcctgcgg cattactact
     6421 acaactactg ggtgacgcag ggcccgcagg tcgaaagcgc ggccaaggaa gtgctcggcc
     6481 aggcgttcgg cgatgtcggc atgtgccagg gaaataatcc gggcaagaca ctggtttggc
     6541 tgcggccttc gatcttctat aatccgctga tgaagcagta tcacggcaag atccgcgcga
     6601 gtgtctattc cggtagcggc aagcccctgg caacctatat cggcgaaggt caggccgatg
     6661 gttatctcga tatgcgcctc gatcgcgaac gcaagatcgc caccgtctac aagctggcga
     6721 tggccaaggt cgccgagaag atgaaagccg acgctgccct gcaacaggaa ctcaagacca
     6781 acgtggcgcc ggacaacgcg ccgagcccat gtgccatggt gaccttgtta cccgcccccg
     6841 ctgtatccgt catgtcgttt taggagaagt tatgtcattg aacaagaaac atatcgttac
     6901 cctctgtctc ggtggcctgt tggtcagcat cggcttgagc gtacaagcac accagggcaa
     6961 gaccgtcact gggctgaaga cgcctgagtc ggtggtcgaa gcgcccgacg ggcgtgtctt
     7021 cgtttccgag atcggcgagt tcggcaagga tggcgacggc gagatcagct atatcgacaa
     7081 ggatggcaag cgccatccat tcgccagcgg catggatgat cccaagggcc tggccttgat
     7141 cggtgacgat ctctacgtgg ccgacaagac ccgcgtactg aaggtcggct ctgacggcaa
     7201 atggcaggtg ttcgcggctg cgacggcatt cccgacgact cccaagttcc tcaacgacct
     7261 ggaggccgac cctcatggca tgctttatgt cagcgatagc ggcaatctgg aaggtgtcgg
     7321 cggcgcgatc tacagcatcg atcgagatgg caaggtgacc ctgctcatcg atggcgccaa
     7381 ggacaaacgc gtgctcgcgc ccaacggcct gctggccgac gatacgggca aggccctgct
     7441 ggaggtggat ttcgtgtcag gcatcctcta taagctcgat ctcgccaccg gtgagatgca
     7501 ggaactggcc agcggcttcg gtggcggcga tggactggtc catcatccca agggcttgat
     7561 gttcgtcagc gactggaaga acggcaaagt gttcagtgtc gatgccgtga aggacgaagt
     7621 gaaactgtta aaagccggtt tcaaggcgtc ggcggacatc gccatgagcc gggatgggcg
     7681 ctacgtcatc gtgccggaca tgaaggcggg cgaagtcgtc tggttgccga tacatatcaa
     7741 gtgatcgttc gatgcaatcg gccgtgcatg acaggcggtc ttgcaggact ggcggtctag
     7801 cgctagccat gaaaaaagcc gcgggtttcg cggctttttt catgggcacc atggcgttat
     7861 gcgcggggtg gcgcgtctgc ttcagatcac cgtgaagaga ttggcgatgc cgtccaggcc
     7921 catctggttc aaggcataag tggcttgctg ctggacgatg ggcttggcgt ggtaagccac
     7981 gccaaccgcg gctaccgaca tcatcttcaa atcgttggcg ccgtcaccga tggcgatcac
     8041 ctgcgaagcc tgcaggccga gctggtcgcg caggccgatg agcgaatccg ccttggcctg
     8101 gccatcgatg atatgtccca gcaccttgcc ggtgagccgg tgatcgacaa tctccagcgt
     8161 gttggcgcga atatggtcga tgccggtcat ggcgcgtacg cgttcggcga agaagtcgaa
     8221 cccgccggac accagcaggg tggtgatgcc gtgctgcttg catgcctgga tccattgcaa
     8281 ggcgccggga ttgagcgtga gtcgttcgtc gatcacgcgc tgcaaggccg attcctccag
     8341 ccccttgagc aacgcgacac gttgacgcag gctctcggcg aattcgatct cgccgcgcat
     8401 ggcgctctcg gtcaccatcg agatctcttt cttgagaccg cacatgtcgg cgatctcgtc
     8461 gatacattcg atgctgatca gcgtcgaatc catgtccatc accgctaggc caaaatgttg
     8521 caaggtctgg ctgtcgggca ccagcgcgca gtcgaggccg tgttctgcgc aataggcgcg
     8581 caaacgcagc agcagcggcg cttgcggttc gctgtcgaat ttaaggtagt aggcgtcggc
     8641 gttgatgcgg gtgaaagcat gcgcttgcgc gatggcggag agtgcttgca gatgctgcgg
     8701 caacatggcc ggatgctgta tgacgaaacg catgggattc caggtcatca aggatttttg
     8761 tgaaccggat tatacaccgt gccagagccg tgccgagggc gttggccgcg ggtatttggc
     8821 tggctaattg ccccgaattc ggccaaaata agctattttg aaaaacaaca aagcatttcc
     8881 atgaatctac acgaattcca ggccaaacac ctgctgcaac actatggttt ggcgatcccc
     8941 aagggtgaag tcgtcgatac cgccgatgcc gccacgctgg ctgccaagca gatcggtggc
     9001 gatgcctggg tggtcaaggc gcagatccac gcgggtggcc gcggcaagtc cggcggcgtc
     9061 aagctggtga ggtccaacgc cgaactgcgt accgcggtcg aaagcctgat cggcaagccg
     9121 ctggtgactt accagaacgc gccggatggc caactggtga cgcgcgtgct ggtcgagcag
     9181 accctgccta tcgcgcgtga actctatctg tcgctgctcg tcgatcgcag tctggaacgt
     9241 gtggtcatgg tggcttccgc ttctggcggc atggatatcg aggagatcgc ggcgcatcac
     9301 cccgagcaga tcctgcagga ggtgtgcgat ccgttgaatg gcctggtgga tttccaggca
     9361 cgcaacctcg cgtttggtct tggcttggtc ggcgaccagg tcgcggcatt cactcggttg
     9421 gccaagggac tctaccgcct gttcaaggag aacgatctgt ccttgctgga gatcaatccg
     9481 ctggtggtca ccaccgatgg cgcgctggtt gcgctcgatt gcaagatcag catcgacgat
     9541 aacgcgctat accgtcagaa ggcgctcgct gagcagcgcg actggagcca ggatgacgcc
     9601 aaggaggcgc aggcgcattc cagcggtctc aattacatcg cgctcaacgg caatatcggc
     9661 tgtatggtca acggcgcagg gctggccatg gcgaccatgg acatcatcaa gctgtatggc
     9721 gccgagccgg ccaacttcct cgacgtcggc ggcggcgcca gcaaggagaa ggtcaccgcg
     9781 gccttcaaga tcatcaccgc cgaccccaac gtgaagggca tcctggtcaa catcttcggc
     9841 ggcatcatgc gctgcgacat catcgcggag ggcgtggtcg cggcggtgaa ggaggtgggg
     9901 ctgaaggtcc cgctggtggt gcggctggaa ggcaccaacg tcaccgaagg caaggccatc
     9961 atcaacaact ccggcctgaa cgtcatcgcg gccaacgacc tggcggacgc cgccgagaag
    10021 gtcgtcaagg ccgtgcggag cgctgcgtaa g
//
LOCUS       DATMTW010000308         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_160030, whole genome shotgun sequence.
ACCESSION   DATMTW010000308 DATMTW010000000
VERSION     DATMTW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160030"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VN084_06045"
     CDS             complement(<1..1446)
                     /locus_tag="VN084_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWT29054.1"
                     /translation="MRFAVSLLTVIGIASIIGTVLKQNEPYANYIIQFGQFWFDFFEM
                     LGLYDVYHSAWFLLILLFLVISTSLCIYRNSPLMLRELQTYRETATEKSLRAFSHKRV
                     YQSIHSAADTRQRLQQFLTLRGFRFKTTQHADGSELIAAKAGSYQRLGYIFTHAAIVI
                     ICIGGLLDGNVPFKVQELLGYKKVETLDIPVSQVAKESRLTTANLSFRANMTLPEGTG
                     ADVAFQRMRDGYLVQELPFRIELKDFRIEHYATGQPKSFESDLLIVDPDLKEPLARTI
                     SVNHPLIYKGIAIYQSDFQDGGTKLKLNVWGLFGTQTKPLAVEGTIFKKTTLGEGDDA
                     LTLEFNDFRLFNILNLSADGKGKPHNVGPSVTFKVRDTQGQAREYINYIQPFLLSGKA
                     YFVSGMRENPQQDFRYLRIPADEDFGIEGFMNLRATLFDQDAHAEIARRFAKAAFGQR
                     PSAELTQQQFEASVKQLLKAFAAGGYTRVADM"
BASE COUNT          344 a          418 c          441 g          336 t
ORIGIN      
        1 catatccgcg actcgggtat acccaccggc cgcaaaagct ttcaacaact gcttcacgct
       61 ggcttcgaac tgctgctgcg tgagctcagc cgacggccgt tgaccgaagg ccgccttggc
      121 gaatctccgc gcgatctcgg catgcgcatc ctgatcgaac aacgtcgccc gcaggttcat
      181 gaacccttcg ataccgaaat cctcatccgc cgggatacgc agataacgaa aatcctgttg
      241 cggattctca cgcatgccgg agacaaaata ggcttttccg ctcagcagga aaggctgtat
      301 gtaattgata tattcgcgcg cctgcccctg ggtatcgcgc accttgaagg tgacgctcgg
      361 gccaacgtta tggggcttgc ctttgccgtc tgccgacagg ttgaggatgt tgaacagacg
      421 gaaatcgttg aattccaggg tcagcgcatc gtcaccttcg ccaagggtcg ttttcttgaa
      481 gatcgtgcct tcgaccgcca gcggtttggt ttgggtgccg aacaggcccc agacattgag
      541 ttttagcttg gtaccaccgt cctggaaatc cgactggtag atggcgatgc ccttgtagat
      601 cagcggatga ttgacgctga tggtgcgggc caaaggctct ttcaagtccg ggtcgacgat
      661 cagcagatcg ctttcgaagg acttgggctg accggtcgcg taatgctcga tacgaaaatc
      721 cttgagctcg atacggaatg gcagctcctg caccaggtag ccgtcccgca tgcgctggaa
      781 tgcgacatcg gcaccagtac cttccggcaa ggtcatgtta gcccggaatg acaggttggc
      841 cgtggtcagg cggctctcct tcgccacttg cgacaccggg atatcgagcg tctccacttt
      901 cttgtagcca agcaactcct gcaccttgaa cggcacattg ccgtcgagca ggccaccgat
      961 gcagatgatg acgatcgcag catgcgtgaa gatgtagccc aatcgctggt agctaccagc
     1021 cttggccgcg atcaattcac taccgtcggc atgctgtgtg gtcttgaaac ggaaaccgcg
     1081 caatgtcagg aactgctgca ggcgctgccg ggtatccgct gcagaatgta tcgattgata
     1141 gacgcgcttg tgactgaagg cgcgtaagga tttctcagtc gcggtttcac ggtatgtctg
     1201 cagctcacgc agcatcaagg ggctgttgcg atagatacag agactggtgg agatcacgag
     1261 gaacagcagg atcaacagga accaggcgct gtggtagacg tcgtacagcc ccagcatctc
     1321 gaagaaatcg aaccagaact ggccgaactg gatgatgtaa ttggcgtaag gctcgttctg
     1381 cttgagtacg gtgccgatga tggaagcgat gccgatcacc gtcagcaagc tgacagcgaa
     1441 acgcatcgag ctcattagct cataaaatcg tctggaggtg gtgattgctg gcttcaaagc
     1501 tggctctgga aaaatttaag ggtggtcacg ccaccctta
//
LOCUS       DATMTW010000309         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_27225, whole genome shotgun sequence.
ACCESSION   DATMTW010000309 DATMTW010000000
VERSION     DATMTW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27225"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VN084_06050"
     CDS             <1..1123
                     /locus_tag="VN084_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWT29055.1"
                     /translation="IMPATSAFAAKAQDFKLNYSGGNGTRIGVFSMMTGVPGSYWPVF
                     LQSHRSAAMIDVLQQQDYQMSFYTSARFSYPEFDQSIFSKVPKDQLHWIDAPLAGWEK
                     DRKNVSDMLQFIDQRDPSRPFFTFMFFESPHARYYFPPESVIRKPYRDDINYATMDRG
                     SLKGDMTLIKNRYLNAVHHLDSQFQRVFDYLEAHQLLDSTIVVAVGDHGEEFMEHGYW
                     GHNSTFSDQQTRTPLVLWIPGQKPAVHEELTSHLDLPATIMPRLGVTNPASDYSVGHD
                     LLSGVHRDHVILSDWSSVGYKDSQVKIRLPMNAGGFASKQITGPHDEMLNIADAQSRF
                     KVAQPELVDMMRELGKFSVKASAKTQAKVASTPAQEQAP"
     gene            1120..>2691
                     /locus_tag="VN084_06055"
     CDS             1120..>2691
                     /locus_tag="VN084_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HWT29056.1"
                     /translation="MSAVTQTPGQPRAFTRPLPFVLVLSLVAVSCWTLLRLFLWLEVG
                     PEELTLRESASSFLLGSWFDLWTLAYLVSPLLLVSSLLGNRLRASFTMQVVRCGVLWL
                     AVAALLFGITAEYLFWQEFTTRFNFIAIDYLIYTNEVIGNIQESYPVGVILGVIGLIS
                     ALVVWLCRQYVQFDNAPRSGIQRLRLLGIALILPLAANIVADLDQTRLGGNNYAQELA
                     GNGLYTLAAAMRRNDLDYDRFYQTMPKTVAQQTLDALGLRRDPAAKLVRYLRPQEASM
                     GPFYKRPRNVVLITVESLSARYLGAYGNTQKLTPNLDRLANEGYRFDKLYATGTRTVR
                     GLEALSLGTPPVPGQAIVRRPHNQHLATVGEYLEAQHYTTQFIYGGYGYFDNMNAYFR
                     GNDYQVIDRTDFDPKHIAAENIWGVADESLFDNTLHVLDQDAAKQRPFFVQIMTTSNH
                     RPYTFPAGRIDLPQGTRKSAVKYTDYAIGRFIAEASTKPWFDDTLFVIVADHCASVAG
                     KTSLPVDKYHIPMIFY"
BASE COUNT          528 a          882 c          764 g          517 t
ORIGIN      
        1 catcatgccg gccaccagcg ctttcgccgc caaggcgcag gatttcaaac tcaactacag
       61 cggcggcaac ggtacgcgca tcggcgtgtt cagcatgatg accggcgtcc ccggcagcta
      121 ctggccggtg ttcctgcagt cacaccgcag tgcggcgatg atcgacgtgc tgcagcagca
      181 ggattatcag atgtcgttct acaccagcgc ccgtttctct taccctgagt tcgaccagag
      241 catcttcagc aaggtaccga aggatcagct gcactggatc gatgcgccgc tcgccggctg
      301 ggaaaaggac cgcaagaacg tcagcgacat gctgcagttc atcgatcagc gcgatccgtc
      361 gcggccgttc ttcactttca tgttcttcga gtcaccgcat gcgcgttatt acttcccgcc
      421 ggagagcgtg atccgcaagc cttaccgcga cgacatcaat tacgcgacca tggaccgcgg
      481 ctcgctcaag ggcgacatga ccctgatcaa gaaccgctac ctgaacgccg tgcatcatct
      541 cgacagccag ttccagcgcg tcttcgatta ccttgaggca catcagctgc tggatagcac
      601 catcgtcgtc gccgtcggcg accacggcga ggagttcatg gagcatggct actggggcca
      661 taactcgacc ttctctgacc agcagacgcg tacgccgctg gtgttgtgga ttccgggcca
      721 aaagccggcc gtgcacgagg agctgaccag ccacctcgat ctgccggcga ccatcatgcc
      781 gcggctgggt gtgaccaatc cggccagcga ttattccgtg ggccatgacc tcttgtccgg
      841 cgtgcatcgc gatcacgtca tccttagcga ctggtcgagt gtcggataca aggacagcca
      901 ggtgaagatc cgtctgccga tgaacgccgg cggtttcgcc agcaagcaga tcaccggtcc
      961 gcatgacgag atgctgaaca tcgccgatgc ccagagccgg ttcaaggtcg cgcagccgga
     1021 gcttgtcgac atgatgcgcg agctgggcaa attctcggtc aaggcatccg ctaaaacgca
     1081 ggcaaaagtg gcgtcgacgc cagcacaaga gcaggctcca tgagcgccgt cacgcagacg
     1141 ccgggtcagc ctcgcgcatt cacgcggcca ttgccgttcg tgctggtctt gagcctggtg
     1201 gctgtcagct gctggacgct gctgcggctg tttctctggc tcgaagtcgg gccggaggag
     1261 ctgaccctgc gtgaatccgc cagcagtttc ctgctcggta gctggttcga cctatggacg
     1321 ctggcttacc tggtcagccc gctgttgctg gtgtcgagcc ttctcggcaa ccgcctgcgc
     1381 gcttcgttca cgatgcaggt ggtacgttgc ggcgtactct ggctggcggt ggctgcgctg
     1441 ttattcggca tcaccgccga atacctgttc tggcaggaat tcaccacccg cttcaatttc
     1501 atcgccatcg actacctgat ctataccaat gaagtcatcg gcaatatcca ggaatcgtat
     1561 ccggtcggcg tgatcctggg cgtgatcggc ctcatctctg cgttggtggt ctggctttgc
     1621 cggcagtacg tgcaattcga taacgcgccg cgcagtggca tccagcgcct gcgcttgctg
     1681 ggtatcgcgc tgatcctgcc tttggccgcc aacatcgtcg ctgatctcga ccagaccagg
     1741 ctaggcggca acaactatgc gcaggaactc gcggggaacg gcctctatac cctagcggcc
     1801 gccatgcgca ggaatgacct cgactacgac cgtttctacc agaccatgcc gaagaccgtc
     1861 gcccagcaga cgctggatgc gctcggcctg cgccgcgacc cggcggccaa actggtgcgt
     1921 tacctcaggc cgcaggaagc gtccatgggg ccgttctaca agcggccgcg caatgtcgtg
     1981 ctgatcacgg tggaaagcct gtccgcccgt tatctcggcg cctacggcaa tacccaaaag
     2041 ctgacaccca acctcgatcg cctggccaac gagggctacc gtttcgacaa gctctacgcc
     2101 accggcacgc gcaccgtgcg cgggttggaa gcgctctcac tcggtacgcc gccagtgccg
     2161 ggccaggcca tcgtgcgccg accccataac cagcacctgg ccaccgtcgg cgaatatcta
     2221 gaagcccagc attacacgac gcagttcatc tacggtggct acggctattt cgacaacatg
     2281 aacgcctact tcaggggcaa cgattatcag gtgatcgatc gcaccgattt cgatcccaag
     2341 catatcgccg ccgagaacat ctggggcgtc gccgacgaga gcctgttcga caacaccttg
     2401 cacgtgctcg accaggatgc cgccaaacag cggccgttct tcgtgcagat catgaccacc
     2461 agcaaccacc ggccctatac cttccccgca gggcgcatcg acctgccgca gggcacacgc
     2521 aagtccgcgg tgaaatacac cgattacgcc atcggccgct tcatcgcgga agccagcacc
     2581 aaaccctggt tcgacgatac gctgttcgtt atcgtcgccg accactgcgc atcggtcgca
     2641 ggcaagacca gcctgccggt ggacaagtac cacatcccga tgatcttcta t
//
LOCUS       DATMTW010000310         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_229075, whole genome shotgun sequence.
ACCESSION   DATMTW010000310 DATMTW010000000
VERSION     DATMTW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229075"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3861
                     /locus_tag="VN084_06060"
     CDS             <1..3861
                     /locus_tag="VN084_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT29057.1"
                     /translation="FAQVTVVCPGAVLNIFRGSFQLPNVEFIESHPEDQSRWHWHSTT
                     MSVPLAMHTTLNNLPADVPYLVAPVNRVQHWAAQLEKLDHGQKPRVGLVWAGGNLLRD
                     DKKRSIPPEQFLPLTAHRELTWVSLQKANDDRKKARSHHADRLIDWMDDIRDFADTAA
                     LIQNLDLVISVDTAVAHLAGALGKSVWMLNRYAGDFRWMFDREDSPWYPGMRIFRQPT
                     PGDWASVLAKVQLALEAWRPGGDEAPKAAATPTLQDDMQRLFDQFTRGVPAYNAIRDR
                     LFADYRQARGIDLSQHYVELLVQFGYGFFKNHNLSQACKFWREVLDLRPDHHYALHHL
                     GVALAALGRHAEACAFFRRAIAVEPERHYAHYCLAASLLALGQWEEGWRYYEYRWYGS
                     DQAMRHRTKKGAISLPDSLDATLKRWRGESPAAGEALLLYAEQGYGDQMQFVRLLPQV
                     AARFARVDLCCSPAVRALFANYLHEVGDVRLIDEIPQDRSAWQWHCPLLSLPLALDLR
                     PDSIPPAPYLTPPADRQTAWSQRLQPYRDHRPRIGFAWSGSAGLGADQQRSIDPLLLI
                     GLLADTRYRWFSLQKADTKTHGVMPESLRPLVIDFMPEVRDFADTAALIAQLDLVISV
                     DTAVAHLAGAMGKPVWLLNRYAGDWRWVGHEDRSPWYSSMRIFHQRIAGQWQEVIARV
                     AQALDTLYPASSDTAADASGHGTTVISASPPGAALTSPAEGVAQHLVALFCSGQADAL
                     AQAAAAVRPTTRDAGLARMMSQVARLLSEQKATPGDALPALQDVRDDAWPADLTRAVS
                     AIHTAAGHFQQCLTTRQDRPLALHKLAAILLTSGLYAAAATYYRQALSLRPDFIVART
                     NLGTAHMRLGQLETALDDYQRALELDPGYVPALNGMAVTCKHLGRFEQAIATFGSALA
                     IQPGNAELLNNLGATLLDMHRVTEAIDLFRRAITADPTYHAAYMGLALALLFNGEWPE
                     GWRYYEHRWQGSNFALQGMVHKTDTNLPHWQGEPVKADARLLVFCEQGLGDNLQFVRY
                     LPLAAARFAQVTLFCPAALHNILAGSFCAYANIEVRKVLPNDHSAWDFHCYIMSMPLA
                     FGTTPDNIPDQLPYLRSPTNRVQHWAERLQASDASGLRVGLVWAGGSRLRDDRQRSIP
                     LTKFAGLLHVKGVTWVSLQKERRAEDADGATAPASLIDWMPEISDFADTAALIDNLDL
                     VISVDTAVAHLAGGLGKPVWLLNRYAGDWRWMYEREDSPWYPGMRIFNQTAHDDWDDV
                     LQRVEARLEALASSAVMTRSLRPV"
     gene            3891..4418
                     /locus_tag="VN084_06065"
     CDS             3891..4418
                     /locus_tag="VN084_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HWT29058.1"
                     /translation="MQTTPSNARLSTMSTILFGSRWLQLPLYLGLIVAQAVYVYHFMM
                     ELLHLVQGIASLNEEHIMLVVLGLIDVVMISNLLIMVIVGGYETFVSRLNLKGHPDEP
                     DWLSHVNANLLKVKLATAIIGISSIHLLKTFINAANLEPKVLIWQTVIHITFVLSAIS
                     IAWIDRLMLHPDQKH"
     gene            complement(4419..>5254)
                     /locus_tag="VN084_06070"
     CDS             complement(4419..>5254)
                     /locus_tag="VN084_06070"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HWT29059.1"
                     /translation="RAPYMLLQFLFGSLLSALFILYFRSASHVMAILWSLGLAGLLVA
                     NEFLEDHYKRQTLSWALLGFCAILLFNFSVPFLIGSIHATWFYCSTLIGALLVHWLHE
                     RSGQAGRIVFVWIIAGVLSLAYLVDAIPPVPLVKRDVQIGLRLESHDGTITLMTESTP
                     FWQRWQPWQDDVRLPPGEKLYCVSSVFAPIGLETQLFHRWQYKNPQRGWVTMSRIGFA
                     LTGGRQQGFRGYTYKQNVQPGSWRVAVETENGRTVSVYHFKVVAADASTMAQLHPYTV
                     D"
BASE COUNT         1027 a         1727 c         1557 g          943 t
ORIGIN      
        1 ttcgcgcagg tcacggtggt ctgtcccggg gccgtgctca atatcttccg cggctccttc
       61 cagttaccca atgtcgaatt catcgaatcc catccggagg atcagtcgcg ctggcactgg
      121 cattccacga cgatgtccgt gcccctggcg atgcatacca cactgaacaa cctgcccgcc
      181 gacgtccctt acctggttgc gccagtgaac cgcgtgcagc actgggctgc acagctggaa
      241 aaactcgacc atgggcaaaa gccgcgcgtc gggctggtct gggccggtgg taacctgctg
      301 cgcgacgaca agaagcgcag tatcccgccg gaacagttcc tgccgctgac cgcgcaccgg
      361 gaactgacct gggtcagtct gcagaaggcc aatgacgaca gaaagaaagc ccgatcgcac
      421 catgcggaca ggctcatcga ctggatggac gatatccggg atttcgccga taccgccgcc
      481 ctgatccaga acctcgacct ggtcatcagc gtcgatacag ccgtggccca tctggccggc
      541 gcactcggca agtcggtatg gatgctgaat cgctatgccg gcgattttcg atggatgttc
      601 gatcgcgagg atagcccatg gtatccaggc atgcgcatct tccgccaacc gacccccggg
      661 gactgggcga gcgtgctggc caaggtgcaa ctggcgctcg aagcctggcg gcccggtggc
      721 gacgaagcac cgaaagccgc agcgacgccg accttgcaag acgacatgca gcgcctgttc
      781 gatcagttca cgcgcggggt tccggcttac aacgccatcc gcgatcgcct gttcgccgat
      841 taccgccaag cgcgagggat cgacctcagc cagcattatg tcgagctgct ggtgcagttc
      901 ggttatggct tcttcaagaa ccataacctg tcgcaagcct gcaaattctg gcgcgaagtg
      961 ctcgacctgc gacccgacca tcattacgcc ttgcatcatc tcggcgtcgc cctcgcggcg
     1021 ctgggtcgcc atgcggaagc ctgcgccttc ttccgccgcg ccatcgccgt cgaacccgaa
     1081 cgccattacg cgcattattg cctcgccgcc tcgctgctgg cgctgggaca atgggaggaa
     1141 ggctggcgct attacgaata ccgctggtac ggatcggatc aggcgatgcg acaccggaca
     1201 aaaaaggggg cgattagcct gccagactcc ctggatgcca cgctcaaacg ctggcgaggc
     1261 gaatcaccgg ctgcaggcga ggcgctgttg ctatatgccg aacagggcta tggcgaccag
     1321 atgcagttcg tgcgcctgct gccgcaagtg gcagcccggt tcgcgcgcgt cgatctctgt
     1381 tgcagcccgg cggtgcgcgc gctattcgcc aactacctgc atgaggtagg cgacgtcagg
     1441 ctgatcgatg agattccgca agaccgcagc gcctggcaat ggcattgccc gctgctctcg
     1501 ctgccgctag cgctagacct gcggcccgac agcatcccgc cggcacctta tctcaccccg
     1561 ccagccgacc gccagacagc ctggtcgcag cgtctgcaac cctaccggga ccacagaccc
     1621 cgcatcggct tcgcctggtc aggtagcgcg gggctaggcg ccgaccagca acgctccatc
     1681 gacccgctgc tcctaatcgg cttgctcgcg gatacgcgct accgctggtt cagcctgcaa
     1741 aaagcggata ccaagaccca tggcgtcatg ccggagagtc tgcggccgct ggtcatcgac
     1801 ttcatgcccg aggtcaggga ctttgccgac actgcggcgc tcatcgcaca attggatctg
     1861 gtcatctcgg tcgataccgc tgtcgcgcat ctggccggcg ccatgggcaa accggtatgg
     1921 ctgctcaacc gctacgcagg cgactggcgc tgggtcggcc acgaagaccg ctcgccctgg
     1981 tatagcagca tgcgcatctt ccatcagcgc atagcgggtc agtggcaaga ggtgatcgcc
     2041 cgtgtggcgc aggcgctgga tacgctttac cccgcctcgt cggacaccgc agcggacgca
     2101 tccggtcatg gcaccaccgt tatctccgcc agcccgcccg gggccgcact gacctccccg
     2161 gcagaaggag tagcccaaca cctggtcgca ctgttctgtt cgggtcaggc ggatgcactg
     2221 gcgcaagccg ctgcagcagt gcggccaacg acgcgtgatg ccggcctggc acgcatgatg
     2281 tcgcaagtcg cgcgtctgct atcggaacaa aaggccacac ctggagatgc gctgccagcg
     2341 ctgcaggacg tacgcgacga tgcctggcca gccgatctca cgcgggccgt ctcggcgatc
     2401 cacaccgccg ctgggcattt tcagcaatgc ctcaccacac gccaggatcg tccactggcc
     2461 ttgcacaaac tggcggcgat actgctgact agcggactct atgcggcggc ggccacgtat
     2521 tatcggcagg cgctgagcct gcgcccggat ttcatcgtag cccggaccaa cctcggcacc
     2581 gcgcacatgc ggctgggcca actggagacc gcgctggacg attaccagcg cgccctggag
     2641 ctagacccgg gctatgtgcc ggctctcaac ggtatggctg tcacctgcaa acatctcgga
     2701 cgcttcgaac aggcaatcgc cacctttgga tccgcgctgg cgatccagcc cggcaacgcc
     2761 gagctgctaa acaacctcgg ggcgaccttg ctcgacatgc atcgggtcac ggaagcgatc
     2821 gacctcttcc gccgcgccat caccgctgac cccacatatc atgccgcgta catggggcta
     2881 gcgttggcgc tgctgttcaa tggcgaatgg cccgaaggct ggcgctacta tgagcatcgc
     2941 tggcagggct ccaatttcgc cttgcaaggc atggtgcaca aaaccgacac caacctgccc
     3001 cactggcagg gcgaaccggt caaggccgac gcccggctgc tggtcttttg cgaacagggc
     3061 ctgggcgaca atctgcagtt cgtgcgctac ctgccgctcg cggcagcccg cttcgcccag
     3121 gtcaccttat tctgcccggc agcgctccat aacatcctgg caggttcctt ctgcgcttac
     3181 gccaacatcg aggtgcgcaa ggtcttgccc aacgaccata gcgcctggga tttccactgc
     3241 tacatcatgt ccatgccgct ggcgttcggc accacccctg acaacatccc cgatcagctg
     3301 ccctatctgc gttcgcccac caaccgggta cagcattggg cagaacgcct gcaggcgtca
     3361 gatgccagcg ggctgcgcgt tgggctcgta tgggcgggcg gcagccggct gcgcgacgat
     3421 cggcagagaa gcatcccgct gacaaaattc gccgggctgc tgcatgtgaa aggcgtcacc
     3481 tgggtcagcc tgcagaagga gcggcgtgcg gaagatgcgg atggcgcgac agctccggca
     3541 tcgctgatcg actggatgcc ggagatctcc gattttgccg ataccgcggc cctgatcgac
     3601 aacctcgatc tcgtcatcag tgtcgacaca gcggtagccc atctcgctgg cgggctcggc
     3661 aagccagtct ggctgctcaa ccgctacgcc ggtgactggc gctggatgta cgaacgcgag
     3721 gatagcccct ggtatccggg catgcgcatc ttcaatcaga ccgcacacga cgattgggat
     3781 gacgtactgc aacgcgtcga agcaaggttg gaagcgctgg cgagctcagc ggtgatgacg
     3841 cgtagcctcc gcccggtata atacgcacgc atttttcgat aaggacatac atgcaaacca
     3901 ccccgagcaa cgcccgtttg agcaccatga gcacgatcct cttcggcagc cgctggctgc
     3961 aactgccgct ctacctgggg ctgatcgtcg cgcaagcggt ctatgtctat cacttcatga
     4021 tggaattgct gcacctggtg cagggcatcg cctcgctcaa cgaggaacac atcatgctgg
     4081 tggtgctggg attgatcgac gtggtgatga tctcgaacct cttgatcatg gtcatcgttg
     4141 gcggctatga gaccttcgtc tcgcgtctca acctcaaggg gcatccggac gagccggact
     4201 ggctgtcgca tgtcaacgcc aacctgctca aggtcaagct ggcgacggcg atcatcggca
     4261 tctcgtcgat ccacctgctg aagactttca tcaacgcggc caacctcgaa cccaaggtgc
     4321 tgatctggca gaccgtgatc cacatcacct ttgtcctgtc tgccatctcc atcgcctgga
     4381 tcgaccggct gatgctgcat cccgaccaga agcactgatc agtcgacggt gtagggatgc
     4441 agttgtgcca tcgttgacgc atccgcggca acgaccttga agtgatagac cgagacggtc
     4501 cgcccgttct cggtctcgac agccacccgc cagctgcctg gctgcacatt ctgcttgtag
     4561 gtgtagccac ggaagccctg ttgtcgcccg cccgtcagcg cgaacccgat acgcgacatc
     4621 gtcacccagc cacgctgcgg attcttgtat tgccaacgat ggaacaactg cgtttccaga
     4681 ccgatgggcg cgaacacgga agacacgcag tagagttttt cgcccggcgg caggcgcaca
     4741 tcgtcctgcc aaggctgcca gcgctgccag aatggcgtgc tctcggtcat cagggtgatc
     4801 gtgccgtcat ggctctccag ccgcaacccg atctgcacgt cacgtttcac caggggtacc
     4861 ggcgggatcg cgtcgaccag atacgccagc gacagcacac cggcgatgat ccagacgaag
     4921 acgatgcgtc cggcctgacc cgaacgctca tgcagccaat ggaccagcaa ggcgccgatc
     4981 agcgtactgc agtagaacca ggtggcatgg atactgccga taaggaacgg cacgctgaaa
     5041 ttgaacagca ggatggcaca gaaccccagt agcgcccagc tcagtgtctg gcgcttgtag
     5101 tggtcctcga ggaactcgtt ggcgaccagg aggccagcca gcccaaggct ccagaggata
     5161 gccatgacat ggctggcgct cctgaaatag agaatgaaca aggcgcttag caagctgccg
     5221 aagaggaact gcagcagcat gtatggcgcg cgca
//
LOCUS       DATMTW010000311         1619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_328667, whole genome shotgun sequence.
ACCESSION   DATMTW010000311 DATMTW010000000
VERSION     DATMTW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328667"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /gene="argS"
                     /locus_tag="VN084_06075"
     CDS             complement(<1..711)
                     /gene="argS"
                     /locus_tag="VN084_06075"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWT29060.1"
                     /translation="MKAHLTELLAQAAKSLSLESADIAIHLERPKTAQHGDFSTNLAM
                     LLAKPLRQNPRAIAERIIAALPDSAYIEKVEIAGAGFINFFLAARARQTVINDIQAQG
                     ARYGHNHNGQGQKLQIEFVSANPTGPLHVGHGRGAAVGDCLARLLAANGWDVTREFYY
                     NDAGAQIDNLTLSVQARCKGISPDDRAFPADGYRGEYIIDIAKAYLAKETVTADDMQV
                     TASGDAEDAESIRKFSVAY"
     gene            815..>1619
                     /locus_tag="VN084_06080"
     CDS             815..>1619
                     /locus_tag="VN084_06080"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWT29061.1"
                     /translation="MSAQPLNPPAHILRVALDVPLDRQFDYLASGIDAEVGQRVVVPF
                     GRRRQVGIVLGHAAQSEFDESRLKPVTQVFGDEPPLDAETLALLKFCADYYQYPYGQA
                     LLAALPTRLRQTEPAVTRKQFLYSLSEDARAAGPTLIPKRQAIQSRIYTALHEHGTLD
                     EGALAACSPGWRKAVSAMQMQGWLEAREIIAGLTPSSASPTLAPALNADQQAACDAIT
                     QSLHDFKPWLLYGITGSGKTEVYMRLIEQVLMDATAQVLVLVPEINLTPQ"
BASE COUNT          313 a          497 c          499 g          310 t
ORIGIN      
        1 gtaggctacg gagaacttgc ggatggactc cgcgtcctcg gcatcgccac tggcggtgac
       61 ttgcatgtcg tcggcggtca cggtctcctt cgccaggtaa gctttggcga tatcgatgat
      121 gtactcgcca cggtagccat ccgcagggaa tgcccgatca tccgggctga tgcccttgca
      181 gcgcgcctgc accgacaagg tcaaattatc gatctgcgca ccggcgtcgt tgtaatagaa
      241 ttcgcgcgtg acatcccagc cattggccgc cagcaggcgt gctaggcaat cgccgacagc
      301 ggcaccgcgg ccatggccga catgcagtgg gccggtcggg ttggcggaga cgaactcgat
      361 ctgcaacttc tgcccctggc cattgtggtt gtggccatag cgcgcgccct gggcctggat
      421 gtcgttgatg accgtctgcc tggcacgcgc ggccagaaag aaattgatga aaccggcacc
      481 ggcgatctcc accttttcga tatacgcgct gtccggcaag gcggcgatga tgcgctcggc
      541 gatcgcccgc gggttctggc gcaaaggctt ggccagcagc atggccaggt tcgtggagaa
      601 atccccatgt tgcgcggtct ttggccgctc aagatggatc gcgatatccg ccgattcgag
      661 ggaaagggat tttgcggcct gggcgagtaa ttcggtaaga tgtgctttca aggcggacga
      721 tcaataaaag tatgaataaa aaagcgtatt ttaacctact gcgtgcacgc gctgaaaacc
      781 cattgtcgaa ttccgccgcg tcacgaccat cctgatgtcc gcccagcctc tgaacccccc
      841 tgcgcatatc ctcagggtcg cgctggatgt ccccctcgac agacagttcg attatcttgc
      901 gagcggcatc gatgccgagg tcggccagcg cgtggtcgtc cctttcggac gccgtcggca
      961 agtcggcatc gtgctcggcc atgccgcaca aagcgaattc gacgagagca gattgaagcc
     1021 ggtgacgcag gtgtttggcg acgagccgcc gctcgatgcc gagacgctgg cgttgctgaa
     1081 gttctgtgcc gactattatc agtatcccta cggccaggcc ttactggcgg ccttgccgac
     1141 acgtctgcgc cagaccgagc cggccgtcac gcgcaaacag ttcctctata gtctatcgga
     1201 ggacgccagg gcggctggcc cgaccctaat cccgaagcgg caggcgatcc agtcacgcat
     1261 ctacacggcg ctgcacgaac acggcacgct ggacgagggc gccttggcag cctgctcgcc
     1321 gggctggcgc aaggccgtat cggcgatgca gatgcaaggc tggcttgaag cgcgtgagat
     1381 cattgccgga ttgacgccat ccagcgcatc gccgacgctg gcccctgccc tcaacgccga
     1441 ccagcaggca gcctgtgacg ccatcacgca gtcgctgcat gacttcaagc cctggctgtt
     1501 gtatggcatc acggggagcg gcaagactga agtctacatg cggctgatcg aacaggtgct
     1561 catggatgcg acagcgcagg tactggtgct ggtgcccgag atcaatctca cgccacaac
//
LOCUS       DATMTW010000312         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_27436, whole genome shotgun sequence.
ACCESSION   DATMTW010000312 DATMTW010000000
VERSION     DATMTW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27436"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VN084_06085"
     CDS             complement(<1..644)
                     /locus_tag="VN084_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWT29062.1"
                     /translation="MLNALYVDFNSYFASVEQQERAKLRGKPVGVLPVMAETTCCIAA
                     SYEAKAFGVKTGTNVREARKLCPDIVFVEARPKLYIEYHHRLVEIVESCTHVERTLSI
                     DEMVCMLTGSQQVRENALALADRIKQAISRQAGDHIRCSIGIGPNVFLAKLASNMQKP
                     DGCVVIEQHELPERLYPLQLRDFNGIGPRMAERLKRQGITTVRQLCDLDRQRLRT"
     gene            complement(685..1254)
                     /locus_tag="VN084_06090"
     CDS             complement(685..1254)
                     /locus_tag="VN084_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HWT29063.1"
                     /translation="MRTRSLLLSIAFVALLAPLPPSAYADPPSWAPAHGWRKKHDPYY
                     VGYSGDHWDRDYGIIEGRCNRQAIGTVLGGAVGGAIGTTADREYRAVAVIVGSVLGAV
                     IGNQIGKSMDEADRGCIGHALELAADRRAVTWRNPDSGLDYKVTPLRGFNDGGHKCRE
                     YRLDINGGGINDSRKERACQTAPGKWEAD"
     gene            1347..1898
                     /gene="msrA"
                     /locus_tag="VN084_06095"
     CDS             1347..1898
                     /gene="msrA"
                     /locus_tag="VN084_06095"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWT29064.1"
                     /translation="MDSNPSRIETAIFAGGCFWCLEPVFSQLRGVMKVVSGYIGGYTV
                     DPTYKQVCTGTTGHAEAIEISYDPHEISFDELLEIFFVAHDPTTPNRQGNDIGTQYRS
                     GVFYLNVAQKEAAERMIAELDAQRLWPDPIVTEVTSAARFYPAEDYHQYYYANNMHQP
                     YCMAVARPKVAKVRAKYADKLKN"
     gene            complement(1903..>2882)
                     /gene="corA"
                     /locus_tag="VN084_06100"
     CDS             complement(1903..>2882)
                     /gene="corA"
                     /locus_tag="VN084_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWT29065.1"
                     /translation="FEYDASEVSERTLTANDAATLAMPGEGKLWLNVHGLHDANLMKQ
                     LGETFKLHPLTMEDILNTTQRPEVDRYDDYLFIVTRFFYYDERRMAISSEQISLVLGK
                     HFVLSFQERATGSFEPIRERLRTNRGHIRGLGVDYLTYALLDMVVDRYFGVLEEMGED
                     CETLEEVLLRKSNAKLLKNIHQLKRSSMELRRAVWPLREVINTLVRNDDKVFTDNTVL
                     YLRDVYDHIVHFIESLESIRDMLGGMMDIYLSSISNRVNMEVRALTVVTMLFMPATLI
                     SGIFGMNFEVMPWLKHPDGFWFALGLMGAIAAMMGLIFWRRQWLSRSQK"
BASE COUNT          617 a          845 c          812 g          608 t
ORIGIN      
        1 gtacgcaacc gttgtcgatc aagatcgcat aattgccgga cagtcgtgat cccttgccgc
       61 ttcagtcttt ccgccatgcg cgggccgatg ccattgaaat cccgcagttg caagggatac
      121 aggcgctccg gcagttcgtg ctgttcgatc acgacacagc catccggttt ctgcatgttg
      181 gaagcgagtt tggccaggaa cacattcggg ccgatgccga tggaacagcg gatatgatcg
      241 ccggcctgtc gcgagatcgc ttgtttgatg cgatcggcga gggctagcgc gttctcccga
      301 acctgctggc taccggtgag catgcagacc atctcgtcga tggacaaggt gcgttccaca
      361 tgggtacagg attcgacgat ctcgaccagt cgatgatggt attcgatata aagcttgggc
      421 cgtgcctcga cgaatacgat gtccggacac aacttgcgtg cttcgcgaac attggtgccg
      481 gtcttcacgc cgaaggcctt ggcttcatag ctggcggcga tacagcaggt ggtctcggcc
      541 atcaccggca acacgcctac aggtttgccg cgtagttttg cacgctcctg ttgctcgaca
      601 gaggcgaagt aggaattgaa gtcgacgtaa agcgcgttta acaaacagcg gcatccgatc
      661 taaacgcagg cgacctgctc gtcatcagtc ggcctcccac ttccctgggg cggtctggca
      721 agcgcgctcc ttgcgggagt cgttgatgcc accgccattg atgtccagcc tgtattcccg
      781 acatttgtga ccaccgtcgt taaagccgcg caggggagtc accttgtaat cgagaccgct
      841 atcagggttg cgccaggtca ccgcgcggcg atccgctgcc agctcgagtg cgtggccgat
      901 acagccacgg tcggcctcat ccatggattt cccgatctga ttgccgatca ctgcgcccag
      961 cacgctgccg acgatgaccg cgacggcgcg atactcgcga tccgctgtcg tgccgatggc
     1021 cccgccgacg gccccaccga gcacggtacc gatggcctgg cgattgcaac ggccttcgat
     1081 gatgccgtaa tcgcgatccc agtgatcacc ggaataccct acataatatg gatcatgttt
     1141 cttgcgccag ccatgcgccg gtgcccacga aggcggatcg gcataagcac ttggcggcaa
     1201 aggtgccagc aaggcgacga aggcgatgga aagcagtagg gatctggtgc gcatgatgaa
     1261 cctttccggg tgggtattgc cgaaattcta atcctgtttc atggtacaaa agaatatttt
     1321 cggatcaagc agaaggaaaa tcagccatgg attccaaccc atcccggatc gagactgcca
     1381 tcttcgcagg tggatgtttc tggtgcctgg aaccggtctt ctcgcaactg cgaggtgtca
     1441 tgaaagtcgt ttcaggctac atcggcggtt acacagtaga ccccacctac aagcaggtct
     1501 gtaccggcac gaccggtcac gccgaggcga tcgagatcag ctatgatccg cacgagatca
     1561 gcttcgatga gttgctggag atcttcttcg tcgcacatga tccgaccacg ccgaaccggc
     1621 aaggcaacga tatcggcacg cagtatcgct ccggcgtgtt ctatctcaat gtggcgcaga
     1681 aagaggctgc cgagcgcatg atcgccgaac tggatgccca acgcctgtgg ccggacccga
     1741 ttgtcaccga agtgactagc gccgcacgct tttacccagc cgaggattac caccagtatt
     1801 actatgccaa caacatgcac cagccttact gcatggccgt ggccaggccc aaggtcgcca
     1861 aggtgcgtgc caaatacgcc gataagctga aaaactagcg ccttacttct ggctacggct
     1921 cagccactgc cggcgccaga agatcaaccc catcatcgca gcgatcgcgc ccattagccc
     1981 cagcgcgaac cagaagccgt ccggatgctt gagccacggc atcacctcga aattcatgcc
     2041 gaagatacct gagattaagg tcgcaggcat gaacagcatg gtgaccacgg tcagcgcccg
     2101 cacttccatg ttgactcgat tgctgatgct ggaaaggtag atgtccatca tgccgcccag
     2161 catgtcgcgg atcgactcca gcgattcgat gaaatgcacg atatggtcgt atacatcccg
     2221 caggtagagc acggtgttgt cggtgaacac cttgtcgtca ttgcgcacca gcgtgttgat
     2281 cacctcgcgc agtggccaca ccgcacggcg caattccatg ctggaacgct tgagctgatg
     2341 gatgttcttc agcagtttgg cgttggattt gcgtagcagg acctcttcca gcgtttcgca
     2401 gtcctcgccc atctcttcca gcacgccaaa atatcgatcc accaccatgt cgagcagggc
     2461 ataggtcagg taatcgaccc cgagacctcg aatatggccg cgattggtgc gcaggcgttc
     2521 gcggatgggt tcgaatgaac cggtcgcgcg ttcctggaaa ctcagcacga aatgcttgcc
     2581 gagcacgagg ctgatctgct cggagctgat cgccatgcgc cgttcatcgt agtaaaagaa
     2641 gcgtgtgacg atgaacagat aatcgtcata gcggtcgacc tcgggtcgct gcgtggtgtt
     2701 gaggatgtcc tccatcgtca gcggatgcag cttgaaggtc tcccccagct gtttcatcag
     2761 attggcgtca tgcaggccat gcacattgag ccagagcttg ccttcgcctg gcattgcaag
     2821 tgttgcagcg tcatttgccg taagcgtccg ctcactgact tcgctggcgt catattcaaa
     2881 ga
//
LOCUS       DATMTW010000313         5468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_261487, whole genome shotgun sequence.
ACCESSION   DATMTW010000313 DATMTW010000000
VERSION     DATMTW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261487"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VN084_06105"
     CDS             <1..416
                     /locus_tag="VN084_06105"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWT29066.1"
                     /translation="HLPEDLKRFRALTSGHHIIMGRKTYESLNRLLPDRTTVIVTRNP
                     AYHVPGALVAHSLPAAVALCAKDPEAFLIGGAELYEEGLKLADTLYLTEVHQAFAGDA
                     EFPAWDRRQWQEVARERHTSVKGLPFSYVTYRRVV"
     gene            complement(540..2780)
                     /locus_tag="VN084_06110"
     CDS             complement(540..2780)
                     /locus_tag="VN084_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HWT29067.1"
                     /translation="MKFRFPVVIIDEDFRSENSSGLGIRVLAKAIEDEGFEVLGVTSY
                     GDLTSFAQQQSRASAFILSIDDEEFIEESPDALDNLRKFVEEIRFRNEEIPIFLHGET
                     RTSRHIPNEILRELNGFIHMFEDTPEFVARYIIREAKTYLDSLPPPFFKALTHYAADG
                     SYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAASE
                     RNAARIFNCDHLFFVTNGTSTSNKIVWNSTVAPGDVVVVDRNCHKSVLHSIIMTGAIP
                     VFLMPTRNHFGIIGPIPKEEFAWENIQKKIQRNPFATDKNAKPRVLTITQSTYDGVLY
                     NVEEIKEMLDGKIDTLHFDEAWLPHATFHDFYGDYHAIGADRPRCKESMIFSTQSTHK
                     LLAGLSQASQILVQDAQDIQLDRDVFNESYLMHTSTSPQYSIIASCDVAAAMMEAPGG
                     TALVEESLMEALDFRRAMRKVGSEWGADWWFKVWGPDDLSEEGIEEREAWMLKAGERW
                     HGFGNLAPGFNMLDPIKATIITPGLDVDGDFSEDCGIPAAIVTKYLAEHGVIVEKTGL
                     YSFFIMFTIGITKGRWNTMVAALQQFKDDYDKNQPLWKVLPEFVAKQPRYERIGLKDL
                     CTQIHEVYKANDVARLTTEMYLSDMVPAMKPTDAFAKMAHRNIDRVQIDELEGRITAV
                     LLTPYPPGIPLLIPGERFNRIIVNYLRFAREFNERFPGFETDVHGLVKEIVDGKACYF
                     VDCVAQ"
     gene            complement(2872..3447)
                     /gene="dcd"
                     /locus_tag="VN084_06115"
     CDS             complement(2872..3447)
                     /gene="dcd"
                     /locus_tag="VN084_06115"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWT29068.1"
                     /translation="MSIKSDKWIRRMAEQHGMIEPFEPKQIKQDAEGKRLVSYGTSSY
                     GYDIRCSKEFKLFTNLNSTIVDPKNFDPNSFVEVNADYCIIPPNSFALARTVEYFRIP
                     RNVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGCAQVLF
                     FEADADDVCETSYKDRGGKYQGQVGVTLPKI"
     gene            complement(3508..4986)
                     /gene="zwf"
                     /locus_tag="VN084_06120"
     CDS             complement(3508..4986)
                     /gene="zwf"
                     /locus_tag="VN084_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWT29069.1"
                     /translation="MKQIDPCTLVLFGASGNLSRVKLMPGLFRLDALGRLPENMAILS
                     VGRGEVSQEAWHADIKGMLDAKFKKGYDQEVFKRFIARNHYHANPPTDPDAFKKMKAV
                     LTNDKVFPQNFAYFLSVRPSDFAQVVDQLASVDLLNEDKYWRRVVIEKPFGTDLPSAK
                     ALQASITKHLKESQIYRIDHYLGKSALQNILLQRFANTVLEPIWNKDYIDHVQITNTE
                     MLGVGDRTQFYDATGALRDMIQSHVLQTLALTTMELPKDLSPDAVRAEKIKVLEAIRP
                     IPVNDLKHHAFRAQYAAGEINGEKVPGYLEELGNPDSVVETYAALKLFIDNPRWQGVP
                     FYIRTAKRLHEADTRIAIRFKKAPLQIGDGQQQNWLIIGIQPRECIKLEIQSKIPGLD
                     INTRTIELDAANRQDGDETVDAYEALLLNLMEGDNSQYLHISEVEAQWRLVDPVIEAW
                     AKDRAPVHQYTAGSRDPEASKVIFEAEDQFWRYSIALGGDQH"
     gene            complement(5137..>5468)
                     /locus_tag="VN084_06125"
     CDS             complement(5137..>5468)
                     /locus_tag="VN084_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HWT29070.1"
                     /translation="LLKGKEEFNLDLAQITELWRHGSVVRSWLLDLTADALKVDQTLD
                     TIVPYVADSGEGRWTVVEAVEQGVAAPVLTVALQARFRSQDAKGYSYKLLSLMRNAFG
                     GHAVKTK"
BASE COUNT         1031 a         1500 c         1730 g         1207 t
ORIGIN      
        1 ggcatctgcc ggaagacctc aaacgcttcc gtgcattgac cagcggccat catattatca
       61 tgggtcgcaa aacctacgag tccctcaacc ggctcctgcc ggaccgcacc acagtcatcg
      121 ttacgcgtaa tccggcgtat cacgtgccgg gcgcgctggt cgcgcattct ctgccggcgg
      181 ccgtcgcgct gtgtgccaag gacccggaag cattcctgat cggcggcgcg gagttgtatg
      241 aagaaggcct gaagctggcg gataccctgt atctgacaga agtgcaccag gcgttcgccg
      301 gcgatgccga gttcccagcg tgggatcggc gacaatggca agaagtcgca cgtgaacgtc
      361 acaccagcgt caagggtctg cctttcagtt acgtcaccta ccgtcgggtg gtttgaaccc
      421 tatcctggcg ggccatcctt gaacgccagc cttgcacgcc aatcccttga ataccagcgc
      481 ttgaatgcca gtctagaacg ctgcccttgc gcgcccgcgc aaggtcgcaa acgctaaggc
      541 tactgggcga cgcagtcgac gaagtaacaa gccttgccgt cgacgatctc tttcaccagg
      601 ccatgcacat ctgtctcgaa accaggaaag cgttcgttga actcgcgcgc gaaccggaga
      661 tagttgacga tgatccggtt gaagcgctca cccgggatca gcaatgggat gcctggcgga
      721 tagggcgtca gcaacacggc ggtgatacgg ccttccagtt cgtcgatctg cacacggtcg
      781 atattgcgat gcgccatctt ggcgaaggcg tcggtcggct tcatcgcggg caccatgtcc
      841 gacaggtaca tctcggtcgt cagacgcgcg acgtcattgg ctttatagac ctcgtggatc
      901 tgagtgcaca ggtccttgag gccgatacgc tcatagcgcg gctgcttggc gacgaactcc
      961 ggcagcacct tccatagcgg ctgattcttg tcgtaatcgt ccttgaattg ctgcagggcg
     1021 gcgaccatgg tgttccagcg acccttggtg atgccgatgg tgaacatgat gaagaaggaa
     1081 taaagaccgg tcttctcgac gatgacgccg tgctcggcca ggtatttggt gacgatggcc
     1141 gcggggatgc cgcagtcttc cgagaaatcg ccgtcgacgt ccagccccgg ggtgatgatg
     1201 gtggccttga tcggatccag catgttgaag ccaggggcga ggttgccgaa gccgtgccaa
     1261 cgttcacctg ccttcagcat ccaggcttcg cgttcctcga tgccttcttc cgacaggtca
     1321 tcaggtcccc agaccttgaa ccaccaatcg gcgccccatt cgctgcccac cttgcgcatg
     1381 gcgcggcgga aatccagggc ttccatcagc gattcttcga ccagcgcggt gccgcccggc
     1441 gcttccatca tggcggctgc cacatcgcag gaggcgatga tggaatattg cgggctggtt
     1501 gaggtatgca tgaggtaaga ctcattgaat acgtcgcggt cgagctggat gtcctgcgcg
     1561 tcctgcacca ggatctgtga agcctgactc aggccagcca gcagcttgtg cgtggattgc
     1621 gtggagaaga tcatcgactc cttgcagcgt gggcggtcgg cgccgatggc atggtaatcg
     1681 ccgtagaaat cgtggaaggt cgcgtgcggc agccaggcct catcgaaatg cagggtatcg
     1741 atcttgccgt ccagcatctc cttgatctct tccacgttat acaacacgcc gtcataggtc
     1801 gactgggtga tcgtcagtac gcgcggcttg gcgttcttgt cggtggcgaa ggggttgcgc
     1861 tggatcttct tctggatgtt ctcccaggcg aactcttcct tggggatcgg cccgatgatg
     1921 ccgaagtgat tgcgggtcgg catcaggaac acggggatcg cgccggtcat gatgatggaa
     1981 tgcagcaccg acttgtggca gttacggtcg acgacgacga catcgccggg cgctacggtt
     2041 gagttccaga cgatcttgtt cgaggtcgat gtgccgttgg tgacgaagaa caggtggtcg
     2101 cagttgaaga tacgggccgc gttgcgctcg gaagcggcca cagggccggt atggtccagc
     2161 aattggccga gttcgtcgac cgcgttgcag acgtcggcac gcagcatgtt ctcgccgaag
     2221 aactgatgga acatctggcc caccggggac ttgaggaacg ccacgccacc cgagtgaccg
     2281 gggcagtgcc aggaatagga gccgtcggca gcgtaatggg tcaatgcctt gaagaacggt
     2341 ggcggcagac tgtcgaggta ggtcttggcc tcgcggatga tatagcgagc gacgaattcc
     2401 ggggtgtcct cgaacatgtg gatgaagccg ttcagctccc gcaggatctc gttcgggata
     2461 tggcggctgg tgcgcgtctc gccgtgcagg aagatcggga tctcctcgtt gcggaaacgg
     2521 atctcctcga cgaacttgcg caggttatcg agcgcgtcag gcgactcctc gatgaactcc
     2581 tcgtcgtcga tcgacaggat gaaggccgac gcgcggctct gctgttgcgc gaacgaggtc
     2641 aggtcgccgt agctggtgac gccgaggact tcgaaaccct catcctcgat ggctttggcc
     2701 agcacgcgga tgccgaggcc tgaagaattt tcagaacgga agtcttcgtc aatgatgacg
     2761 acagggaatc ggaatttcat cagggcctcc taacggctgc cgataaaaag aatgggacgg
     2821 aatatagcac gctattgtgc tgtgaccatg acttgggcaa ttcgatctgg atcagatctt
     2881 gggcagggtg acgcctactt gcccttgata cttaccgccg cgatccttat aggaagtttc
     2941 gcagacatcg tccgcgtcag cttcgaagaa caatacctgc gcacaacctt cgttggcgta
     3001 gatcttggcc ggtagtgggg tggtgttgct gaattccagc gtgacgtagc cttcccattc
     3061 cggttcgaac ggggtcacgt tgacgatgat gccgcaacgg gcataggtgg attttccgag
     3121 gcagacggtg agtacgttac gcgggatacg gaagtactcg acggtgcgcg ccagcgcgaa
     3181 cgagttcggc gggatgatgc agtaatcggc attcacttca acgaaggaat tggggtcgaa
     3241 attcttgggg tcgacgatgg tgctgttgag gttggtgaac agcttgaact ccttcgaaca
     3301 gcggatatcg tagccgtagc tcgaggtgcc gtaggagacc aggcgcttgc cttcggcgtc
     3361 ctgcttgatc tgtttcggct cgaacggctc gatcatgccg tgctgctcgg ccatgcggcg
     3421 tatccacttg tcagacttga tgctcatcgt gatcccttgt gcattgatgg caggcgcatt
     3481 gaccatgcgc ctgcttgtta tgggtgctta atgctggtcg ccgccgaggg cgatgctgta
     3541 gcgccagaac tggtcttcgg cttcgaagat gactttggaa gcttccgggt cgcggctgcc
     3601 cgcggtgtac tggtggaccg gcgcacgatc cttggcccag gcttcgatca ccgggtcgac
     3661 caggcgccat tgggcttcca cttcgctgat gtgcaggtat tgcgagttat cgccttccat
     3721 caggttcagc agtagggcct cgtaagcgtc gacggtctcg tcgccatcct ggcgattggc
     3781 cgcgtcgagt tcgatggtgc gggtgttgat gtccaggccc gggatcttgg actggatctc
     3841 gagcttgatg cattcgcgtg gctggatgcc gatgatcagc cagttctgct gctggccatc
     3901 gccgatctgc agtggcgcct tcttgaagcg gatggcgatg cgcgtgtcgg cttcgtgcag
     3961 gcgcttggcc gtgcggatgt agaacggcac gccttgccag cgcggattgt cgatgaagag
     4021 cttgagcgcg gcgtaggttt cgaccacgct gtccgggttg cccagttctt ccaggtagcc
     4081 cggtaccttt tcgccattga tctcgccagc ggcatactgt gcgcggaagg cgtgatgctt
     4141 gagatcgttg accgggatcg ggcggatggc ttccagcacc ttgatcttct cggcgcgtac
     4201 cgcatccggg ctgaggtcct taggcagttc catcgtggtc aaggccaggg tctgcaacac
     4261 atgactctgg atcatgtcgc gcagcgcgcc ggtggcgtca tagaactggg tgcggtcgcc
     4321 cacgcccagc atctcggtat tggtgatctg gacgtgatcg atgtaatcct tgttccagat
     4381 cggctctagc acggtgttgg cgaagcgctg cagcaggatg ttctgcaggg cggatttgcc
     4441 gaggtagtgg tcgatccggt agatctggct ttccttcagg tgcttggtga tcgaggcctg
     4501 caacgccttg gcggaaggca ggtcggtgcc gaatggcttc tcgatgacca cgcgacgcca
     4561 gtatttgtct tcgttcagca ggtcgacgga cgccagctgg tcgaccactt gtgcgaagtc
     4621 ggaaggacgc acagaaagga agtaagcgaa attctgcgga aagaccttgt cgttggtcag
     4681 cacggctttc atcttcttga acgcatccgg atcggtcggc gggttcgcgt ggtaatggtt
     4741 acgtgcgatg aagcgcttga acacttcctg gtcgtaaccc ttcttgaact tggcatcgag
     4801 catgcccttg atgtcggcgt gccaggcttc ctgactcact tcaccgcggc cgacggaaag
     4861 aatcgccatg ttttctggca ggcggccgag cgcgtccagt cggaacaggc cgggcatgag
     4921 tttgacgcgg gagaggttac cgctggcgcc aaacaaaacc agggtacatg ggtcgatttg
     4981 tttcatgggt gcaagttttc cgtaggtcta atttcgaatc aaagctagtt cgtgatttta
     5041 cagcttttcg cccgctttta ataatggccg gcgcataaag gaaaatggcg cctttgcggg
     5101 tggcaaacgc gccattctcg ggcagtgaaa accgggttac ttggttttga ctgcgtggcc
     5161 gccgaatgcg ttacgcatca aggagagcag cttgtagctg taacccttgg cgtcctggct
     5221 tctgaatcgg gcttgcaggg ccacagtcaa cactggcgca gcgacacctt gctcgacggc
     5281 ttcaaccacg gtccagcgac cttcgccaga gtcggcgacg taagggacga tggtatcaag
     5341 cgtctggtcg actttcagtg cgtccgcggt caggtcgagc agccagctgc gcacgacgga
     5401 accgtgacgc cagagctcgg tgatctgcgc gaggtcgagg ttgaactctt ccttgccctt
     5461 cagcaggt
//
LOCUS       DATMTW010000314         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_497068, whole genome shotgun sequence.
ACCESSION   DATMTW010000314 DATMTW010000000
VERSION     DATMTW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497068"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..681)
                     /gene="pgsA"
                     /locus_tag="VN084_06130"
     CDS             complement(115..681)
                     /gene="pgsA"
                     /locus_tag="VN084_06130"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWT29071.1"
                     /translation="MFWNVPNSLTVLRIVLIPVFVALFYLPTDILASHWLNLTATLVF
                     ALAAVTDWLDGYLARVLNQTSAIGAFLDPVADKLMVAAALIVLVEFERVGALVALIII
                     GREIAISALREWMAGVGQSGSVAVAFIGKVKTAAQMAAILFLLYYDPVGGLDIKFIGE
                     VLIDIAAILTLLSMAYYLRAAWPAMKAR"
     gene            complement(682..2514)
                     /gene="uvrC"
                     /locus_tag="VN084_06135"
     CDS             complement(682..2514)
                     /gene="uvrC"
                     /locus_tag="VN084_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWT29072.1"
                     /translation="MPDENPVFDPKPILKNLPGLPGVYRMINAQDEVIYVGKAKDLKK
                     RVSSYFVKNLPSPRTRMMVSNIARIETTVTRSEAEALLLESNLIKGLMPRYNVLFRDD
                     KSYPYIALTGDDFPRLAFHRGTQRKGNDYFGPFPNAVAVRESIQLLQKVFLLRTCENT
                     VFANRSRPCLLYQIERCTAPCVGFISPEEYARDVSHAAMFLQGKEQQVMDALEAKMTD
                     AARAQEYERAAVYRDRMQSLRQVQAKQFVSDFNVSDADVIACTEQAGHYCINLVMIRG
                     GRHLGDKSFFPRNTEDCDAAMTVEAFLAQHYAGQAIPPLIIVGVPLETADLEAVFSEQ
                     ANRKIRINTNPVGDKRVWMKMAQTNAELALQQRAAQAANQEARLAALREALDIPESTE
                     RIECFDISHTMGEATVASCVVFDKGAMQNAEYRRYNITGITPGDDYAAMRDALTRRYT
                     KIAAGVAAGEAVKVPDLIFIDGGKGQLGVAVDVMQEVGLPDILLVGIAKGEERKPGLE
                     QLFFPDRETPVSLKKDHAGLHLLQQIRDEAHRFAITGHRARRGKARMHSSLEDIGGIG
                     AKRRQRLLTRFGGLEGVKNASVDELAQVEGISQMLAEKIYQELH"
     gene            complement(2544..2726)
                     /locus_tag="VN084_06140"
     CDS             complement(2544..2726)
                     /locus_tag="VN084_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HWT29073.1"
                     /translation="MPYINVKLAGSLTREQKDKIAKEITDTMERIAHKPKSYTYIVFD
                     EVAEENWAVSGDLLDQ"
     gene            complement(2727..3383)
                     /locus_tag="VN084_06145"
     CDS             complement(2727..3383)
                     /locus_tag="VN084_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWT29074.1"
                     /translation="MDAAHTMTPIFDSQCRRCDRLASFLDGVRSSHPDYYARPVPPFG
                     DAHPRLLIVGLAPGMHGANRTGRPFTGDYAGVLLYETLHKFGYASRPQSVSADDGLVL
                     SGCRISNAVKCLPPENKPTGGEINTCNRYLAAELAALPSATVVLALGNIAHQAVLKAL
                     GLKVGAYKFGHAARHALPSGMVLYDSYHCSRYNTQTRRLTEPMFHQVFESIDTELRRT
                     "
     gene            complement(3398..>4027)
                     /locus_tag="VN084_06150"
     CDS             complement(3398..>4027)
                     /locus_tag="VN084_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWT29075.1"
                     /translation="VGGIVYEALARLQHPEPVRGLEVMVIAALGLVINVIVALRLHRH
                     QGNLNHRAALLHVLGDLLGSVAALVAGGIIYFTGWTPIDPILSLFISALILVSTVRLL
                     REVLHVLMEGVPHHIEIDQVTARLAAIPKVHEIHSVHVWALSSEVTALSAHVVLEDIN
                     AWHEVLTACRTVLQAEFDIDHVTLQPETVSALSNGEVACWLIQPHTHSS"
BASE COUNT          800 a         1242 c         1199 g          786 t
ORIGIN      
        1 gaactcgcga cctcaacctt ggcaaggttg cgctctacca actgagctat tcccgcgtaa
       61 agagacgtgc attttaatga ttctcggcgg gctgtcaaca ccgaaacgcg cctcttatct
      121 agctttcatg gcaggccatg ccgctctcag gtagtaagcc attgacagca atgtcaaaat
      181 cgccgcgatg tcgatcagca cctccccgat aaatttgata tcgaggccac cgactgggtc
      241 gtaatagagc aaaaacagga tcgcagccat ctgtgccgcg gtcttcacct tgccgatgaa
      301 agcgaccgcg acgctaccgg actgccctac gccagccatc cattcacgca gtgcgctgat
      361 ggcgatctcg cggccgatga tgatgagcgc gaccagcgcg ccgacgcgtt cgaactcgac
      421 caggacgatc aacgcggcag ccaccatgag cttgtcggcg accgggtcga ggaacgcgcc
      481 gatggccgaa gtctggttga gcacgcgtgc caggtagccg tccagccagt ccgtcaccgc
      541 agccaacgca aacaccagcg tcgccgtgag gttgagccag tgcgacgcga ggatatccgt
      601 cggcaggtag aacaacgcga cgaataccgg gatcagcacg atccttaata ccgtcaacga
      661 gttggggacg ttccagaaca tctagtgcag ctcctgatat attttttccg ccagcatctg
      721 gctgatgcct tccacttggg cgagctcgtc gacgctcgcg ttcttcacac cctcaagccc
      781 gccgaaacgg gtgagcagtc gctgcctgcg cttggcgccg atgccaccga tatcttccag
      841 cgacgaatgc atgcgcgcct tgccacgacg ggcacggtgc ccagtgatgg cgaagcgatg
      901 cgcttcgtca cggatctgct gcaacaggtg caggcccgca tggtccttct ttaggctgac
      961 gggcgtctcg cgatcgggga agaacaattg ctccaggccc ggcttacgct cctcgccctt
     1021 ggcgatacct accagcagga tatcaggtaa gccaacctcc tgcatgacat cgaccgcgac
     1081 gccgagctgg cccttgccgc cgtcgatgaa gatcaggtcc ggcactttga cagcctcgcc
     1141 cgccgccacg ccggctgcga tcttggtata acgacgggtc agcgcatcgc gcatagcagc
     1201 atagtcgtcg cccggcgtga tgccagtgat gttgtaacgc cgatactccg cgttctgcat
     1261 cgcacccttg tcgaacacca cgcacgacgc cacggtggct tcacccatgg tatggctgat
     1321 gtcgaagcat tcgatgcgct cggtggattc cgggatatcc agcgcttccc gcaaggcggc
     1381 cagccgcgct tcctggttcg ccgcctgtgc cgcccgctgc tggagcgcca gctcggcgtt
     1441 ggtctgcgcc atcttcatcc agacacgttt atcgcctacc gggttggtgt tgatgcggat
     1501 cttgcggttg gcctgctcac tgaacacagc ttccagatcc gcggtttcca gcggcacgcc
     1561 gacgatgatc agcggcggga tggcttgccc agcataatgt tgtgccagga aagcttcgac
     1621 agtcatcgcc gcgtcgcagt cctcggtatt gcgcgggaag aaactcttgt cgcccagatg
     1681 ccgtccgcca cggatcatga ccaggttgat gcagtaatga cccgcctgct cggtacaggc
     1741 gatgacatcg gcatcgctga cattgaaatc gctgacgaac tgcttggcct gcacctggcg
     1801 caagctctgc atgcggtcgc gatacacggc ggcgcgctcg tattcctggg ctcgggcggc
     1861 atccgtcatc ttggcttcaa gcgcgtccat cacctgctgc tccttgccct gcaggaacat
     1921 cgccgcatgg ctgacatcgc gtgcgtattc ctctggcgag ataaagccga cgcagggtgc
     1981 cgtacagcgc tcgatctggt agagcaggca aggccgcgag cggttggcga atacggtgtt
     2041 ctcacaggtg cgcagcagaa agaccttctg cagtaactgg atgctctcgc gcactgccac
     2101 cgcattcggg aacggcccga agtaatcgtt gcccttgcgc tgggtgccgc gatgaaaagc
     2161 cagccggggg aaatcatcgc cggtcagcgc gatataagga taggatttgt catcgcgaaa
     2221 caagacgttg taacgcggca tcaagccctt gatcaggttg ctctcgagca gcagggcttc
     2281 ggcttcggag cgcgtgacag tggtctcgat gcgcgcgatg ttggatacca tcatgcgcgt
     2341 gcgcggcgac ggcaggttct tgacgaaata ggatgagacc cgcttcttca gatccttggc
     2401 cttgccgaca tagatcacct catcctgcgc gttgatcatg cggtatacgc cgggcaggcc
     2461 cggcaggttc ttcaggatcg gcttggggtc gaatacggga ttttcgtcag gcatggcaag
     2521 gcttcaacgg cgtagcgacc ggctcactgg tccagcagat ctccagacac cgcccagttc
     2581 tcctccgcca cctcatcgaa cacgatatag gtataggact tgggcttgtg cgcgatgcgc
     2641 tccatggtat cggtgatctc cttggcgatc ttgtcctttt gttcacgcgt cagggaaccg
     2701 gcaagcttga cattgatata aggcattcac gttctcctca gttcggtgtc gatcgactcg
     2761 aatacctgat ggaacatggg ttccgtcagg cgcctggtct gcgtattgta gcggctgcag
     2821 tggtagctat catagagcac catgccgctg ggcaaagcat ggcgcgcggc atggccgaac
     2881 ttgtaggcgc cgaccttcag ccctagcgcc ttcaataccg cctggtgcgc gatattaccc
     2941 agcgccagaa ccactgtggc ggacggcaac gctgccagtt cggcggcaag gtaacggtta
     3001 caggtattga tctcgccacc ggtcggcttg ttctccggcg gcaggcattt gaccgcgttg
     3061 ctgatgcgac aaccgctcag caccaggcca tcgtcggcag aaaccgactg cgggcggctg
     3121 gcatagccga atttgtgcaa cgtctcatag agcagcaccc cggcatagtc gccggtgaaa
     3181 ggccggccgg tgcgattggc gccatgcatg cccggcgcca ggccgacgat cagcaacctt
     3241 ggatgcgcgt caccgaacgg cggcaccggt cttgcgtaat agtctggatg gctggatcgg
     3301 acaccgtcga gaaagctggc gagccgatcg caacgtcggc attggctatc gaagataggc
     3361 gtcatggtgt gggcggcatc catcgctgac ctcccgctca cgaagaatgc gtatgcggct
     3421 ggatgagcca gcaggcaact tcaccattac tgagtgccga cacggtctcg ggctgcaagg
     3481 tgacgtggtc gatgtcgaac tccgcctgca acaccgtgcg gcaggcggtc aatacctcat
     3541 gccaggcgtt gatgtcttcc agcaccacat gtgccgagag cgccgtgact tccgacgaca
     3601 aggcccagac atggacgctg tggatctcgt gcaccttggg gatggcagcc agacgggcgg
     3661 tgacctggtc gatctcgata tggtgcggca ccccttccat cagcacatgt agcacttcgc
     3721 gtagcagccg cacggtcgag accaggatca gggcggagat gaacagcgac aggatggggt
     3781 cgatcggcgt ccaaccggtg aaataaatga tgccgccggc caccagcgct gccaccgagc
     3841 caagcaggtc acccagcaca tgcaacagtg cggcccgatg gttgagattg ccttggtgcc
     3901 gatgcaaccg cagggcgacg atgacattga tgaccagccc cagcgccgcg atcaccatca
     3961 cttccaggcc acgcaccggt tccggatgct gcaggcgcgc cagcgcctca tagacgatcc
     4021 cgccgac
//
LOCUS       DATMTW010000315         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_363997, whole genome shotgun sequence.
ACCESSION   DATMTW010000315 DATMTW010000000
VERSION     DATMTW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363997"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VN084_06155"
     CDS             <1..794
                     /locus_tag="VN084_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWT29076.1"
                     /translation="TYLGFNLKHKPFDDVRVRQAINYAIDKQELIDGVLLGLGEPVSS
                     PYKPGTRWTNPKLKPYPYDTAKARALLQAAGYEDHDGDGILDKNGKPLSFEILTNQNK
                     QREMSAVLIQRRLKEIGIDVSIRVLEWASFVGRYIKTGDFDVVLLGWGLSLDPDQYSI
                     WHSSQQAPGQFNFIGYNNPRVDRLLEQGRLELDPDKRMAIYHEFAEILLADSPIVYLF
                     AGYGLPAIHKRVKGIDDPAPPAGIGHNSYAWYIPSALRRKEITAE"
     gene            791..1810
                     /locus_tag="VN084_06160"
     CDS             791..1810
                     /locus_tag="VN084_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWT29077.1"
                     /translation="MIGYLLKRLFWMIPMLIGISLISFLIMHLAPGDITTTESGFNPK
                     ASEESRQKLRELYNLDKPVIVQYGLWLHRMVRLDFGTSFASHQRPVFWETKDAEGNVT
                     KGMIQEALPVTLLINLLSLGLIIVVALPLGVISSLTQNRWPDRSITLFVFVGFAIPGF
                     WLALLLMYWTGVAHDWLPISGLHSLDYDNLGIFGQLGDTLRHLALPVFISALSGLAGI
                     SLFVRNGMLEVLHQDYITTARAKGLAENTVVYRHALRNALLPLITLLGLAIPGLIGGS
                     VIAESIFAIPGMGKLFYDAVLMRDFPVIMGILTIGSALTLLGNLLADLAYAWADPRVR
                     RGVMR"
     gene            1807..>2621
                     /locus_tag="VN084_06165"
     CDS             1807..>2621
                     /locus_tag="VN084_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWT29078.1"
                     /translation="MSRDHVIIKALSNPLALVGFVIILGIAVVAVLAPYIAPYDPNAI
                     DVKAILLEPSALHYMGTDGLGRDVFSRMLHGARISLLVGIVAVGIATAIGVVLGALAG
                     YYRGWVDILIMRTVDVMLSIPTFFLILAVIAFLTPSIWNIMIVIGLTSWMGVTRLVRA
                     EFLSLREREFVMASQTLGAKDRRLIFRHLLPNSLTPIIVSSVLGVASAVLVESGLSFL
                     GLGVQAPQASWGNILTDGKEYILFAWWLSLFPGLAILITVLGYNLLGEGLRDAL"
BASE COUNT          492 a          793 c          735 g          601 t
ORIGIN      
        1 acacctatct ggggttcaat ctaaagcaca agcctttcga cgatgtgcgg gtacgtcagg
       61 ccatcaatta cgccatcgac aagcaggaac tgatcgatgg ggtactgctc ggcctcggcg
      121 agccagtctc ctctccttac aaaccgggga cacgctggac caacccaaaa ctcaagcctt
      181 atccctacga tacggcaaaa gcccgggcat tgctgcaagc cgccggttat gaagaccatg
      241 atggcgacgg catcctcgac aagaacggca aaccgttgag tttcgagata ctgaccaacc
      301 agaacaagca gcgtgagatg agtgcggtac tgattcagcg gcggttgaag gagatcggta
      361 tcgacgtcag cattcgtgtg ctcgagtggg cgagctttgt cggccgctat atcaagaccg
      421 gcgatttcga tgtcgtgctg ctcggatggg gcctgtcgct cgatcctgac caatacagca
      481 tctggcattc ctcgcagcag gcacccggcc agttcaattt catcggttac aacaatcccc
      541 gggtcgatcg tttactggag caaggccgtc tagaactcga tccggacaaa cgcatggcga
      601 tctatcatga attcgctgag atcctgctcg cggacagccc tatcgtctac ctgttcgccg
      661 gctatggtct accggcgatc cacaagcgcg tgaaaggcat cgacgaccct gccccaccgg
      721 ccggtatcgg ccataattcc tacgcgtggt atatcccgag tgcgctgcgc cgcaaggaga
      781 tcacggcaga atgatcggct accttctgaa acggttgttc tggatgatac cgatgctgat
      841 cgggatcagc ctgatctcgt tcctgatcat gcacctcgct ccgggcgaca tcacaaccac
      901 ggagtcgggc ttcaacccga aggccagcga ggagtcacga caaaagttgc gcgaactcta
      961 taacttggat aaaccggtta tcgtgcaata tggcttgtgg ctgcatcgca tggtacgcct
     1021 cgatttcggc acttcattcg ccagccatca gcgccccgtc ttctgggaaa ccaaagatgc
     1081 cgaaggcaat gtgaccaaag gcatgatcca ggaagccctg cctgtgacct tgcttatcaa
     1141 tctattgagc ctgggcttga tcatcgttgt ggccttgccg ctgggggtga tctcctccct
     1201 gacgcagaat cgctggccag accgctccat caccctgttc gttttcgtcg ggttcgccat
     1261 ccccggcttc tggcttgccc tgctactcat gtactggacc ggcgtggcgc atgactggtt
     1321 gcccatctcc ggcttgcata gcctggatta cgacaacctc ggcatcttcg gccaactcgg
     1381 cgacaccttg cggcatctgg ccctgcctgt gttcatctcc gccttgagcg gcctggccgg
     1441 tatctcgcta ttcgtacgca acggcatgct ggaagtgttg caccaggatt acatcactac
     1501 agcccgcgcc aaaggcttgg ctgagaacac tgtggtctat cgccacgcct tgcggaatgc
     1561 actgttgccc ctgatcacgc tgctgggcct ggctatccct ggcctgatcg gcggctctgt
     1621 gatcgctgaa tcgatctttg ccatccccgg catgggcaag cttttctacg acgcggtatt
     1681 gatgcgtgat ttcccggtca tcatgggcat cctgaccatc ggttctgccc tgaccttgct
     1741 aggcaatctg ctcgcggatc tggcctatgc ctgggctgat ccgcgcgtgc gcaggggggt
     1801 catgcgatga gtcgcgacca cgtgatcatc aaggccctgt ccaatccgct ggcactcgtc
     1861 ggcttcgtca tcattctcgg catcgcagtc gtggcagtgc ttgcaccgta tatcgcgcct
     1921 tacgacccga acgcgatcga cgtcaaggcg atcctgcttg agccttcagc attgcactac
     1981 atggggacgg acgggctggg gcgcgatgta ttctcacgca tgctgcatgg cgcgcgcatc
     2041 tccctgctgg tcggcatcgt tgccgtgggc atcgcgacgg ccatcggcgt cgtgctcggt
     2101 gcgttagcgg gatactaccg gggctgggtc gatatcctca tcatgaggac ggtcgatgtc
     2161 atgctgtcga tcccgacctt tttcctgatc ttggctgtga tcgcgttcct tacgccgtcc
     2221 atctggaaca tcatgatcgt catcggcctc acatcctgga tgggcgtgac acgcctggta
     2281 cgcgccgaat tcctcagtct gcgcgaacgt gagttcgtca tggcctcgca gacgctggga
     2341 gcgaaagacc gtcggctcat cttccgccac ttgttaccga acagcctgac gccgatcatc
     2401 gtcagctcag tgctgggtgt cgccagcgcc gtactggtag agtcgggcct gagcttcctc
     2461 gggctcggcg ttcaagcgcc acaggcttcc tggggcaaca tcctgaccga cggcaaggag
     2521 tacatcctgt tcgcctggtg gctttcgctg ttccctggtc tcgccatcct cattacggtg
     2581 ttggggtata atttgctcgg tgaagggttg agagatgcgc t
//
LOCUS       DATMTW010000316         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_396255, whole genome shotgun sequence.
ACCESSION   DATMTW010000316 DATMTW010000000
VERSION     DATMTW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396255"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VN084_06170"
     CDS             complement(<1..422)
                     /locus_tag="VN084_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWT29079.1"
                     /translation="MTAPDSTSVATGSLNRAVTLLTTIARGSRKGSTLSELVARSGLP
                     RPTIHRVLDSLIALGWVERDAQSARYNLGSDLAAMGYSAIARHPIARIADTTLSRLAE
                     TLDQQVYLSVRSGLDMVCIGRYDSQSQIQIGRGYVGMRG"
     gene            568..1224
                     /locus_tag="VN084_06175"
     CDS             568..1224
                     /locus_tag="VN084_06175"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWT29080.1"
                     /translation="MSDIYGAQHRAMQAQFDTTRLADRVSEQIITPEIPAEHRGFIES
                     RDFFFLTTIDHRGFPTCSYKGGDQGFVRVLDDKTLAFPSLDGNGMYLSMGNITGNNKV
                     GMLFIDFETPHRVRVHGTATVSRDDPLRETFHEAELVVRVEISEIFVNCPRYIHRYQR
                     TAASPYVPQPGCPTPKAQWKRIDEMQDALPARDSDLAHQLGGVITLEEYFEMVANGKG
                     "
     gene            1247..1789
                     /locus_tag="VN084_06180"
     CDS             1247..1789
                     /locus_tag="VN084_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HWT29081.1"
                     /translation="MTRINAACLSAAVLTAVVLAMAQPFPALPVWSLFITWACFFHLG
                     GDQDPMAAFRVTVTHIGLGVVTAWLTALLLFANPWQASGFGQLWGPVVIGLVIGVLVR
                     LSVIGYFAVTPAIIYGYASVWAFLSIGEHFNLQVLQSFSLQNVIVAIPPCVLVGAIMG
                     YINVRMVNLLSKAEHIALEP"
     gene            1945..>2389
                     /locus_tag="VN084_06185"
     CDS             1945..>2389
                     /locus_tag="VN084_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWT29082.1"
                     /translation="METLAPQNQLADWRAHALQLEQAVQSVIVGQDKAIRHINIAIFA
                     RGHVLLEGGVGVGKTTLLQSIARGIGGAYERIEGTVDLMPNDLVYHTYLGNDGRPQID
                     EGPLLRAGEQLSVFFFNEINRARPQVHALMLRVMAERHLHAFKREY"
BASE COUNT          458 a          772 c          682 g          477 t
ORIGIN      
        1 ccgcgcatgc cgacatatcc gcgaccgatc tggatctgtg actggctgtc atagcggccg
       61 atgcagacca tatcgaggcc ggagcgcacg ctcagataca cctgctggtc cagggtctcc
      121 gcgagtcggc tcaacgtggt gtcggcgata cgcgcgatgg ggtgacgagc gatcgccgaa
      181 tagcccatcg ccgcgaggtc gctgccgagg ttgtagcgtg ccgattgtgc gtcgcgctcg
      241 acccagccga gtgcgatgag gctgtcgagc acacgatgga tggtcggtcg tggcaatccg
      301 ctgcgcgcca cgagctcgga cagcgtactg cccttgcgcg agccgcgtgc gatcgtcgtc
      361 agcaaggtca ccgcccgatt caggctgccc gtagccacgg atgtgctgtc tggtgctgtc
      421 atgtctcgcc tcgtttttta tgtccgaata ccggacaact taatcatgcc tgcggattga
      481 ccgcattaaa gcatgattat taatattgac catataaaaa ataacggcta gtccggctat
      541 cggactagcg ttttcgagga gtacaccatg agtgacatct acggcgccca gcatcgcgcc
      601 atgcaagccc agttcgacac cacccgactg gcggaccggg tcagcgaaca gatcatcacg
      661 cccgagatcc cggcggagca tcgggggttc atcgaatcgc gcgatttctt cttcctcacg
      721 accatcgatc atcgcggttt ccctacctgc tcctacaaag gcggtgacca gggtttcgtc
      781 cgcgtgctgg acgacaagac cctggcgttc cccagcctgg acggcaacgg catgtatctc
      841 tccatgggca acatcaccgg caacaacaag gtgggcatgc tgttcatcga tttcgagacg
      901 ccgcatcgcg tccgcgtcca tggcaccgcc acggtgagtc gtgatgaccc gctgcgggag
      961 accttccacg aggccgagct cgtcgtgcgt gtcgagatca gcgagatatt cgtcaattgc
     1021 ccgcgttata tccaccgcta ccagcgcacg gcagcctcgc cttacgtgcc gcaaccgggt
     1081 tgccccacac ccaaggctca gtggaagcgc atcgacgaga tgcaggatgc gctgccggcg
     1141 cgcgatagcg acctcgccca tcaactgggt ggcgtcatca cgctggagga atatttcgaa
     1201 atggtcgcca acggcaaggg ctgacacacc catcgggaga atcaggatga cacgcatcaa
     1261 cgctgcctgt ttgagtgccg ccgtactgac cgccgtggtc ttggcgatgg cgcagccctt
     1321 cccggcactg ccggtctggt cattgttcat cacctgggcc tgcttcttcc acctgggcgg
     1381 cgaccaggac cccatggccg ccttccgcgt cacggtgacg catatcggcc tgggcgtggt
     1441 cactgcctgg ctgacggccc tgctgctgtt cgccaacccg tggcaagcca gcgggttcgg
     1501 ccaactctgg ggacctgtcg tcatcgggct cgtgatcggc gtgctagtgc gcctgagcgt
     1561 gatcggatac ttcgccgtga caccggccat catctacggc tatgccagcg tctgggcctt
     1621 tctcagcatc ggcgagcact tcaacctgca ggttttgcag tcgttctccc tgcaaaacgt
     1681 catcgtcgcc atcccgcctt gcgtgctcgt cggcgcaatc atgggctaca tcaatgtgcg
     1741 catggtcaac ctgctcagca aagcggagca catcgccctc gaaccctgat cacgaccggc
     1801 ttcgggccta gctaccagtc cggtcatcac aacgcgccgg catgatgcat aggtttacgc
     1861 ttgtgcaatc gcaaaaatca cggtaaggtg tatacaggtc tgtatatttt gtcacatgtt
     1921 cattcaatca cccatcagga acccatggaa actctagccc cgcagaacca actcgccgat
     1981 tggcgcgccc acgcgctgca acttgagcaa gccgtgcaaa gcgtcatcgt cggccaggac
     2041 aaggccatcc gccatatcaa tatcgccatc ttcgcccgcg gccacgttct gcttgaaggc
     2101 ggcgtcggcg tcggcaagac cacgctgctg caatccatcg cccgtggtat cggcggcgcc
     2161 tatgagcgga tcgagggcac cgtggacctg atgccgaacg acctcgtcta ccacacctat
     2221 ctgggcaacg acggccgccc gcagatcgac gaaggtccct tgctgcgtgc cggcgagcag
     2281 ctttccgtgt tcttcttcaa cgagatcaat cgcgcccgtc cgcaagtcca tgcgctgatg
     2341 ctgcgcgtga tggcagagcg ccatctgcac gccttcaagc gtgaatacc
//
LOCUS       DATMTW010000317         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_62077, whole genome shotgun sequence.
ACCESSION   DATMTW010000317 DATMTW010000000
VERSION     DATMTW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62077"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /gene="rfaE1"
                     /locus_tag="VN084_06190"
     CDS             complement(<1..1440)
                     /gene="rfaE1"
                     /locus_tag="VN084_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HWT29083.1"
                     /translation="MSQQRLIDTVREQFGQRGPWVLVIGDLMLDRYLIGDVQRISPEA
                     PVPVVLLRQHHERAGGAANVAANLAGLGIETRMAGCIGDDAEGRALLMMLDGDDIRSD
                     AVFVSSHRPTVTKTRILGGHQQMLRLDQESAAAFSEDETRALQALLSSQLQDRPAMVI
                     LSDYAKGVLGSTICRDVIGQASQLGIPVLVDPKGRDYGKYEGATALTPNKRETAEACG
                     ASMDDHTALLSGAKKLRDALGLEFLAVTRGEEGISLIDGNNILHIPATAKQVFDVSGA
                     GDTVISTLAAGMIHGLNHIEALQLANIAAGIVVGKVGTVPISREELLHELISQSSAVQ
                     ADKICDLSGLLTRVAAWKAAGQRIVFTNGCFDLLHAGHVTYLEAARKTGDRLILGLNT
                     DRSVSALKGPTRPVIHEADRARVLAALEAVDAVILFDEDTPLQLIEAVKPDVIVKGSD
                     YTEDQVVGGQQVRSWGGKIALIPIVPGRST"
     gene            complement(1437..2003)
                     /locus_tag="VN084_06195"
     CDS             complement(1437..2003)
                     /locus_tag="VN084_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HWT29084.1"
                     /translation="MNDIDTLRERLAEHRAVFAEMDALLPAIAEVAEVWRDCIAKGGK
                     IILMGNGGSAADSQHIAAEIVGRYKKERRGLPAISLTVDTSALTAIGNDYGFDAIFSR
                     QVEAHCNPGDVVVGLSTSGNSANVIRAIEKARDLGGITVLLSGGSGGKLAEMCDYKLV
                     MPSHDTPRVQEAHIFVYHSLCELLEATH"
     gene            complement(2013..>3005)
                     /gene="rfaD"
                     /locus_tag="VN084_06200"
     CDS             complement(2013..>3005)
                     /gene="rfaD"
                     /locus_tag="VN084_06200"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HWT29085.1"
                     /translation="IIITGGAGMIGSMIAWHVNHFLGRDDIIIVDRFTHPDQWQNLSH
                     RRYANYLDKDELLPWLNDPARGNGERIEAVIHMGAISATTERDFNKLVQDNIRYSQQL
                     WAWCAQRDVPFLYASSAATYGAGENGYDDDETELDQLRPLNGYGYSKQFFDQWALRQV
                     KHRLPTPPRWYGFKFFNVYGPNEYHKERMASVAFHSFNQFREFGTVKLFKSAKEGYAD
                     GMQLRDFVYVKDAAAVVAHFLQHAGDSGIYNIGSGEARAFKDLATAVMTSLGKKPSIT
                     YIDMPQDLQGKYQYFTEANIAKLRAAGYDKSFHTLEEGVADYVQHYLTQPDPYC"
BASE COUNT          539 a          878 c          962 g          626 t
ORIGIN      
        1 agtgctgcgg cccggcacga tcgggatcaa agcgatcttg ccgccccagg atctcacttg
       61 ctgaccaccg acgacctgat cttcggtgta gtcactaccc ttgacgatga catccggttt
      121 tactgcttcg atcagctgca agggggtgtc ttcgtcgaaa aggatgacgg catcgactgc
      181 ttccagcgcg gccaacacgc gtgcacggtc ggcttcgtgg atgacaggcc gggtcggccc
      241 cttgagagcg ctgaccgaac gatccgtatt aaggcccagg atcaggcgat cgccggtctt
      301 gcgcgccgcc tccaggtagg tcacatgccc cgcgtgcagc agatcgaagc agccgttggt
      361 gaagacgatg cgttgtccgg ccgctttcca cgcggcgaca cgcgtcagca ggcctgacag
      421 gtcgcagatc ttgtcggcct ggacagctga gctttgcgag atgagctcat gcagcagctc
      481 ctcacgcgag atgggtacgg tgccgacctt gccgaccacg ataccggcgg cgatattggc
      541 cagctgcagc gcctcgatgt ggttgaggcc atggatcatg ccggccgcca gtgtactgat
      601 gacggtgtcg ccagcaccgg agacatcgaa cacctgtttc gccgtggccg ggatatgcag
      661 gatgttattg ccgtcgatca gcgagatccc ttcttcaccg cgggtcacgg caaggaattc
      721 gaggccgagc gcgtcgcgca gtttcttggc gccagagagc agcgccgtat ggtcatccat
      781 gctggcgcca caggcttctg ccgtctcgcg cttgttcggc gtcaatgcgg tagcgccctc
      841 atatttgcca taatcgcggc ccttggggtc gaccaatacc gggataccca gctggctggc
      901 ctggccgatg acatcccggc agatcgtgct gccgagcacg cccttggcgt aatcggacag
      961 gatgaccatg gcaggtcgat cttgcagttg gctgctgagc aatgcctgca gcgcccgggt
     1021 ctcgtcttct gaaaaggccg cggcgctctc ctggtcgaga cgcagcatct gctggtgtcc
     1081 gccgaggata cgtgtcttgg tcacggtggg gcgatgactg gatacgaaga cggcatcgga
     1141 gcggatgtcg tccccgtcca gcatcatgag cagcgcccgg ccttcggcat cgtcaccgat
     1201 gcagccggcc atgcgcgttt cgataccgag ccctgcgaga ttggccgcga cattggctgc
     1261 tccgccagcg cgttcgtgat gctgccgcag caaaaccaca ggcaccggcg cctctggcga
     1321 gatgcgctgt acgtcgccga tgaggtagcg gtcgagcatg aggtcgccga tgaccagcac
     1381 ccacgggccg cgctggccga actgttcgcg gacggtgtcg atgagtctct gctggctcaa
     1441 tgcgtggcct ccagcaactc acacaggctg tgataaacga agatgtgcgc ttcctgcacg
     1501 cgcggggtgt cgtgtgacgg catgacgagc ttgtagtcgc acatctcggc gagttttccg
     1561 ccgctaccgc cggacagcag gacggtgatg ccgcccagat ccctggcttt ctcgatggca
     1621 cggatgacgt tggcgctgtt gcccgaagtg gagagaccga cgaccacgtc gcccggattg
     1681 cagtgggctt cgacctggcg cgagaagatg gcatcgaagc catagtcgtt gccgatggcg
     1741 gtcagcgcgg aggtgtccac ggtgagggag atggcgggca agccgcggcg ctccttcttg
     1801 tagcggccga cgatctcggc ggcgatgtgc tgactgtcgg cggcgctgcc gccgttcccc
     1861 atgaggatga tcttgccacc tttggcgata caatcgcgcc agacttcggc gacttcggcg
     1921 atcgcaggca gtaacgcgtc catctccgcg aaaacggcgc gatgctcggc aaggcgttca
     1981 cgtaaggtgt cgatgtcgtt cacggttttt cctcaacagt acgggtcagg ctgtgtcagg
     2041 tagtgttgca cgtaatcggc cacaccttct tccagcgtgt ggaatgattt gtcatagccg
     2101 gcggcacgca acttggcaat gttggcttcg gtgaaatact ggtacttgcc ttgcaggtcc
     2161 tgcggcatgt cgatgtaggt gatcgacggc ttcttgccca gactcgtcat cacggctgtc
     2221 gccagatcct tgaacgcgcg cgcctcgccg ctaccgatgt tgtagatccc ggaatccccg
     2281 gcgtgctgca ggaagtgtgc gaccacggcg gcggcatctt tgacgtagac gaaatcacgc
     2341 agttgcatgc catcggcata gccttccttc gcgcttttga acagtttcac agtcccgaac
     2401 tcgcggaact ggttgaagct gtggaaagcg acgctggcca tacgttcctt gtggtattcg
     2461 ttgggtccgt agacattgaa gaacttgaaa ccgtaccagc ggggcggggt cggcagccgg
     2521 tgttttacct ggcgtaaagc ccactggtcg aagaactgct tggaataacc gtagccattg
     2581 agcgggcgca attggtcgag ctcggtctcg tcgtcatcgt agccattttc gccagcaccg
     2641 tatgtggcgg cggagctggc atagaggaat ggcacatcgc gctgtgcaca ccatgcccac
     2701 agttgctgac tgtaacggat gttgtcctgg accagcttgt tgaaatcacg ttcggtggtg
     2761 gcgctgatcg cgcccatgtg gatcaccgcc tcgatgcgtt caccattacc gcgcgcggga
     2821 tcattgagcc agggcaggag ctcgtccttg tcgaggtaat tggcatagcg gcggtggctg
     2881 aggttttgcc attgatccgg atgcgtgaag cgatcgacga tgatgatatc gtcccggccg
     2941 aggaagtgat tgacgtgcca ggcgatcatg ctgccgatca tgccggcgcc gccggtgatg
     3001 atgat
//
LOCUS       DATMTW010000318         9906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_632086, whole genome shotgun sequence.
ACCESSION   DATMTW010000318 DATMTW010000000
VERSION     DATMTW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632086"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /gene="mnmD"
                     /locus_tag="VN084_06205"
     CDS             complement(<1..1241)
                     /gene="mnmD"
                     /locus_tag="VN084_06205"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HWT29086.1"
                     /translation="MPLAPPDQRLVWRDGQPYSAEFGDIYFSSDNGLEETRHVFLQHN
                     RLAQRWQTLTAVHFTIVETGFGTGLNFLCAWALWRKSAPKTARLHFVSVERYPLTHED
                     LRRALALWPELAELSTSLLEAYDTTAPGWHRLQFDAGRVCLTLVIGDALDMLPRLKAG
                     VDAWFLDGFAPARNPEMWQDALFREMARLSRPGATFATFTSAGAVKRGLRAAGFEVLK
                     TAGFGRKREMLCGSLSEDIQASLATPDHAATGMKRTTAPVPCALALPDAAGKAAPHAI
                     VIGAGSAGASSAHALARRGWQVTLLERHEAVAREASGNPQAMLYPRLAGQDIVLSRLA
                     MHGYLNTLRLITSCGLSQAHYAQCGLLQTAFDADERVRCEAVAARRLPETLVHYVDAE
                     TASDIAGLPLQHSALYFPKGG"
     gene            1386..1940
                     /locus_tag="VN084_06210"
     CDS             1386..1940
                     /locus_tag="VN084_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWT29087.1"
                     /translation="MAHFSHLINADIPKPGAVEAPSDWVREHARLFKPGSRVLDLACG
                     HGRNARWLASQGWQVEAVDRDADALRSLQGIAGIRTTLADLEAAAWPYPDTLFDGIVV
                     CRYLHRPLLVTLAESLAPGGVLIYETFMAGQAQFGRPHNPAFLLQPDELHDTYASRLT
                     VVAFEQGRFDHPKPAMLQRICACK"
     gene            2022..3056
                     /locus_tag="VN084_06215"
     CDS             2022..3056
                     /locus_tag="VN084_06215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWT29088.1"
                     /translation="MIPVPIPANEAERLASLHRMQILATPGEAAFDKITHVTQFIFKT
                     PVVYITIMDEHRQWFKSAIGLGIKETSREESFCAHVVYHGEIMVIENAVEDERFVDHP
                     AVCSGIKLRFYAGRPLRNHEGFVIGTLCIVDNKLRSLTAEEIKIFDYLGSWVESVFSG
                     RGLSTVVGSLLEELDATKRDSMVDPLLNVWNRRAIMDIFKKEADLASRQQSALSVLMI
                     DVDNFKRINDVYGHGVGDRVLARIADTLRMSLRTYDSVGRYGGEEFLIILPHTELDQG
                     LKLAERLRSEVANQPIPVHSESIRCTISIGIRSLKAGEESSLKDNLIADADHALLRAK
                     DLGKNRVEQL"
     gene            complement(3071..4345)
                     /locus_tag="VN084_06220"
     CDS             complement(3071..4345)
                     /locus_tag="VN084_06220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWT29089.1"
                     /translation="MNYPPVRDTLTMKLGHKKRSAWLQDLARSMVFARLREIHGGRIH
                     IVEDGVTEDFGHEPGPACKIHVHDPRFYGEIAFGGSIGAGEAYMLGYWTCDNLTNLIR
                     VMALNQQAMDSLEGGFAWLTRPLLKSLHWLNRNTRDGSRRNIAAHYDLGNAMFQLFLD
                     PTMMYSSAIFDTPDMSLEQASISKLDRICRKLALKPEDHVLEIGTGWGGFAIHAAGRY
                     GCKVTTTTISQAQYDLACERVASAGLSHIVTILLKDYRELEGQFDKLVSIEMIEAVGH
                     QFYDTYFATCARLLKPDGMFLLQAITIADQRYYAARKSVDFIQRYIFPGSCIPSVTAL
                     LDSVTRASDLRLFNLEDIGPHYARTLAEWRKNFFANIEQVRALGYSEEFIRMWEFYLC
                     YCEGGFEERALGDVHMLLVKPGNRRASIHPPL"
     gene            complement(4342..5148)
                     /locus_tag="VN084_06225"
     CDS             complement(4342..5148)
                     /locus_tag="VN084_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HWT29090.1"
                     /translation="MLASAIYTGRVRHRRFLPVAHAFRYRLFMMYLDLDELPTVFAGR
                     WFWSIGRANLACFRREDYYGDASLPLKTAIADLVHRETGRRPGGPIRVLTHLRYFGHC
                     FNPVSFYYCFAPDGERLEAIVAEITNTPWKERHAYVLDCAREPAQLPYRFAFSKDFHV
                     SPFMPMEMEYDWAFGRPGPQLDVHMRNIRSDGKQFDATLEMTRQPLTGAALAKVLLAY
                     PWMTVTVVFAIYWQALRLWLKRVPFYPHPKKQGFEPTTQERTTDYARKRT"
     gene            complement(5138..6388)
                     /locus_tag="VN084_06230"
     CDS             complement(5138..6388)
                     /locus_tag="VN084_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWT29091.1"
                     /translation="MKIAIIGSGIAGNTAAYHLHQAHDVTVFEAGNHVGGHTHTHHIG
                     LLGEDHAVDTGFIVFNDRTYPNFIQLLDTLGVASQPSEMSFSVRCEKTGLEYNGTSLN
                     ALFAQRRNLLRPRFYRMIRDILRFNTECLELLANGSEIRLGDYLATKGYGQDFVDLYI
                     VPMGSAIWSTDAKSMLDFPARFFVRFFHHHGMLTVNDRPQWRVIKGGSASYVDQLIKG
                     FRSNIRLNNPVLKVRRLKRSVRVTSLHGGEEAFDWVFFACHSDQALAMLHDPSPQERE
                     ILAAIPYQENNVFLHHDERMMPRRKLAWAAWNYHVTPTPSDRVAVTYNMNILQGLRSK
                     QPLLVTLNHTDQINPAKVIKRLTYHHPVYTTAGAAAQARHAEISGHNRTAYCGAYWRN
                     GFHEDGVVSALAALKHFEEATHAG"
     gene            complement(6401..7363)
                     /locus_tag="VN084_06235"
     CDS             complement(6401..7363)
                     /locus_tag="VN084_06235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HWT29092.1"
                     /translation="MMPAEPKRPFLLRLLRWLDSGAASESNAVENKVDWLRVLPFLLM
                     HLACFAVIWVGWSAFAVVFAAVIYAVRMFAITAFYHRYFSHKAFNTSRLGQFVFALFG
                     ATAAQRGPLWWASMHRHHHAHSDRQEDAHSPVRHGFFWSHLGWFLSDAHFATRTERVK
                     ELAKYPELRLLDRFDVIVPLLFALAIYGLGAWLETAAPGLGTNGWQLLVWGFVISTVV
                     LYHATFSVNSLAHGWGGRRYETPDHSRNNWLIALFTLGEGWHNNHHHYPGSARQGFYW
                     WELDLTYYGLRMLAAMGLIWNLRGVPQEIRESRKVRRDTTANKG"
     gene            complement(7360..7911)
                     /locus_tag="VN084_06240"
     CDS             complement(7360..7911)
                     /locus_tag="VN084_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWT29093.1"
                     /translation="MKRLRRALMAACATLLTSCASQNLPAIATVPQVDLQRFMGDWYV
                     IACIPTAIETEAYNAVESYRLLEDGTIDTVFTFRSGSIDGKPKRYNPHGFVQPGTGNA
                     LWGMQFIWPIKAEYRIAYLNPDYTQTVIARNARDYVWIMARTPSISDDDYQRLVKFVA
                     DLGYDTAKLRKVPQDWTLSPERK"
     gene            complement(7944..8567)
                     /locus_tag="VN084_06245"
     CDS             complement(7944..8567)
                     /locus_tag="VN084_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HWT29094.1"
                     /translation="MRLIIALFLLFCALPSQAREQEWAFKVYLDKSLIGQHVFRLDTQ
                     GEDKTLSSAARFKVKVLFIEAYSYIHDAQEQWHGDCLTSLESRTVENKEISDVKGALQ
                     DDGFKLNGPKGALSLPQCVMTFAYWNPQMLKQRKLLNPQTGEWLDVRIQSLGMEEIEV
                     RGQKTQAEHYRLEATKMKIDLWYGKDQQWLGLQSTTPEGYHIIYKLK"
     gene            complement(8568..9353)
                     /locus_tag="VN084_06250"
     CDS             complement(8568..9353)
                     /locus_tag="VN084_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWT29095.1"
                     /translation="MFDWSIYLGGLAGMMVFGVLGWLLSLWRNNVTHVDSMWSLFFLL
                     AALLYAWHTWFEGARLTLVIVLVDLWALRLCGYLTLRNWGPHEDHRYVVIRRNNEPYF
                     WLKSLYIIFGLQALLAWIVSLPLLGAIHSSGELNWLDLVGMVVMLFGLAWESVADWQL
                     AKFKANSANRGKVLDQGLWAYSRHPNYFGECCVWWGFYLIAVAAGAWWSLPGPLLMTL
                     LLLKVSGVALLEKDIGERRPAYAAYIARTNAFFPGPQKQARDR"
     gene            complement(9346..>9906)
                     /locus_tag="VN084_06255"
     CDS             complement(9346..>9906)
                     /locus_tag="VN084_06255"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HWT29096.1"
                     /translation="AIICSSGRADMLVNLVGFQLGWFACVLGAAYGHPLLGPIVALPI
                     IGWHLARQADKGMELRLLLLTVLIGSAFDQSLMLLDLVRYSANLWQAGWLPIWMMTLW
                     ALFATTLNVSLRWLERRPAIAILFGLVGGPLAYWGGARLGAIQWQQPIALLIALAIGW
                     AVLMPVLGKLASTLRARRLTAGALHV"
BASE COUNT         1994 a         2870 c         3118 g         1924 t
ORIGIN      
        1 cagccgcctt tggggaaata aagcgccgaa tgttgcagtg gcaggccggc gatgtcgctg
       61 gcggtttcgg cgtcgacata gtggaccagc gtttccggca agcgacgtgc cgccaccgct
      121 tcgcagcgga cgcgctcgtc agcgtcgaac gcggtctgca atagcccgca ttgcgcataa
      181 tgagcctgcg agaggccgca actggtgatg aggcgtaacg tattgaggta accgtgcatg
      241 gcgagccgac tcaatacgat gtcctggccg gcaaggcgcg ggtagagcat ggcctgcgga
      301 ttacccgagg cttcgcgtgc gaccgcctcg tgacgctcga gcagtgttac ctgccagcca
      361 cgacgggcca gcgcatgagc gctcgaggcc ccggcgctgc cagcgccgat cacgatggca
      421 tgcggtgctg ccttgccagc agcatccggc agggctagag cgcatgggac aggggccgtc
      481 gtgcgcttca tgccggtagc ggcatgatcg ggggtggcta ggctagcctg gatatcctcg
      541 gaaagacttc cgcagagcat ttcgcgcttg cggccgaacc ctgctgtctt gagcacttca
      601 aaaccggcgg cgcgcaagcc acgcttgacc gcgccggcac tggtgaatgt ggcgaaagtg
      661 gcgccggggc gggaaagtcg tgccatttcc cggaacaggg cgtcctgcca catctcgggg
      721 ttccttgccg gtgcgaagcc gtcgaggaac cacgcatcga ctcctgcttt caaacgcggc
      781 agcatgtcca gtgcatcgcc gatgaccaac gtgaggcaga cgcgccccgc atcgaactgc
      841 aggcgatgcc agccaggcgc cgtcgtgtcg taagcctcaa gcaaggacgt gctgagctcg
      901 gcgagttctg gccagagggc gagtgcccgg cgcaagtctt catgcgtgag cgggtagcgc
      961 tcgacgctga caaagtgcag cctggccgtc ttgggagccg atttgcgcca taatgcccag
     1021 gcacagagga aattgaggcc ggtgccgaag ccagtctcga cgatggtgaa gtgcaccgcg
     1081 gtcagggttt gccagcgctg cgccagtcga ttgtgctgca ggaacacgtg acgggtctct
     1141 tccaggccgt tatcgctgga gaagtagatg tcgccgaatt cggcggaata gggttgtccg
     1201 tcacgccata cgaggcgttg atccggtggg gcaagcggca tgtgactccg ggttcgatgc
     1261 gatgagaggc gggcatggtg gccgacagcc tcttttgcca gagattatgc atgaaaagcg
     1321 cctgctggcg cagtaccgcg cgatgacgag gctcgtcaac agcgctgatg gaaaggatcg
     1381 ccggaatggc acatttcagt catttgatca acgcggacat accgaagccg ggtgccgtcg
     1441 aagcgccctc ggactgggtt cgtgaacacg cccggctatt taagccaggc agccgcgtgc
     1501 tcgatctcgc ctgcggccat ggccgcaatg cgcgctggct ggcttcgcag ggctggcagg
     1561 tcgaggccgt ggatcgtgat gccgacgcgc tacgatcgtt gcagggcatc gccggcatcc
     1621 ggaccacgct ggccgatctg gaagcggctg cctggcctta tccagatacg ctgttcgacg
     1681 gcattgtcgt ttgtcgctac ctgcaccggc cgcttttggt gacattggcg gaaagcctcg
     1741 cgcctggcgg cgtgctgatc tacgagacct ttatggcggg gcaagcccaa ttcggtcgac
     1801 cgcacaatcc ggcgtttctg ttgcagccag acgagctgca tgacacctat gcaagtcggc
     1861 tgaccgtggt cgcattcgaa caggggcgct tcgaccatcc gaagccggcc atgctgcagc
     1921 ggatctgcgc atgcaaatga cgctcatatc cgatgaataa cggctaggca taaggcggca
     1981 agaagttcag aataccgatt aagactgaat agaacagagc catgatccct gtcccaatcc
     2041 ctgccaatga agccgaacgc ctggcatcgc tgcatcggat gcagatcctc gccactccgg
     2101 gtgaggcggc gttcgacaag atcacacatg tcacccaatt catcttcaag acgccggtgg
     2161 tttacatcac gatcatggac gagcaccggc aatggttcaa atccgccatc gggctgggca
     2221 ttaaagagac atcgcgagag gagtctttct gcgcccatgt ggtgtaccac ggcgaaatca
     2281 tggtgatcga gaatgccgtc gaggatgaaa ggttcgtcga tcatcccgcg gtatgttccg
     2341 gtatcaagct gcgattctac gcaggccgtc cgctacgcaa tcacgaaggt ttcgtgatcg
     2401 gcaccctatg catcgtcgac aacaagctgc ggtctctcac cgcagaagaa atcaaaatct
     2461 ttgattacct cggcagctgg gtagagtcgg tgttttccgg cagaggcctg agcaccgtcg
     2521 tcggcagctt gctggaagag ctggatgcca cgaaacggga cagcatggtt gatccgctgt
     2581 tgaacgtctg gaaccgtcgc gcgatcatgg atatcttcaa aaaggaggcg gatctggcga
     2641 gccggcagca atcggctctt tctgtgctca tgatcgatgt cgataatttc aagcgaatca
     2701 atgacgttta cggtcatggc gtaggcgata gggtgctggc caggatcgcg gatactctgc
     2761 gtatgtccct gcgcacctat gattctgtcg gccgctatgg cggcgaggag ttcctgatca
     2821 tattgccgca taccgaactc gaccaaggtt taaagctcgc cgagcgcttg cgctccgagg
     2881 tcgctaatca gccgatccct gtacattcag agtcgatccg ttgcaccatc agtatcggta
     2941 tccgcagcct gaaggcaggc gaagagtcat cattgaaaga taacctcatc gccgatgctg
     3001 accacgcact acttagagcg aaggatctgg gcaagaaccg cgtagagcag ttgtagcgtc
     3061 gattcgtctt ctacagcggc gggtgaatgg acgcacgacg gttgcccggc ttcaccagca
     3121 gcatgtgcac atcgcccagg gcgcgttcct cgaagccacc ctcgcaataa cacaggtaga
     3181 actcccacat gcggatgaat tcttccgagt agcccagcgc gcgcacctgt tcgatgttgg
     3241 cgaagaagtt cttgcgccat tctgccaggg tgcgtgcgta atgcggaccg atgtcttcga
     3301 ggttgaacag ccggagatcg gaagcccgcg tgactgaatc gagcagcgct gtgacggaag
     3361 ggatgcagct gccgggaaag atgtagcgct ggatgaagtc cacggacttc cgcgccgcgt
     3421 agtaacgctg gtcggcgatg gtgatggcct gtagcaggaa catgccgtcc ggtttcagca
     3481 ggcgggcaca ggtcgcgaaa taggtgtcgt agaactgatg cccgacggct tcgatcatct
     3541 cgattgaaac cagtttgtca aattgtcctt ccaactccct gtaatctttc aataatatcg
     3601 ttacaatgtg cgataggccg gcactggcga cacgttcgca agccaggtca tactgcgcct
     3661 gcgagatggt ggtcgtcgtc actttgcagc cgtatcgacc ggcggcatgg atggcgaatc
     3721 cgccccagcc ggtgccgatc tccagcacat ggtcttctgg cttcagggcc agcttgcggc
     3781 agatgcgatc gagcttgctg atcgacgcct gttccaaaga catgtccggc gtatcgaaga
     3841 tcgccgacga atacatcatg gtcggatcca ggaacagctg gaacatggcg ttacccagat
     3901 catagtgcgc tgcgatattg cggcggctgc catcgcgcgt attgcggttg agccagtgca
     3961 ggctcttgag caatggccgg gtgagccagg caaagccgcc ctcgagacta tccatcgcct
     4021 gctggttgag agccatgacg cggatcagat tggtcaggtt gtcacaggtc cagtaaccca
     4081 gcatgtaagc ttcaccagcg ccgatggagc caccgaacgc gatctcgcca tagaagcgcg
     4141 gatcgtgcac atggatcttg caggccggac ccggttcatg gccgaaatct tccgtcacgc
     4201 cgtcctcaac gatatggatg cggccgccgt ggatctcgcg caatcgcgcg aacaccatgg
     4261 accgggcaag atcctgcagc caggcggaac gtttcttgtg ccccagtttc atggtgaggg
     4321 tgtcgcggac gggcgggtag ttcatgtacg tttccttgcg taatcagtgg ttcgctcttg
     4381 cgtggttggc tcgaacccct gctttttcgg gtgtgggtag aacgggacgc gcttgagcca
     4441 gagtctcagc gcctgccagt agatggcgaa cacgaccgtg acggtcatcc acggataagc
     4501 gagcaggacc tttgccagcg cggcgccggt cagtggctgg cgtgtcattt ccagcgtcgc
     4561 gtcgaactgt ttgccgtcac tgcggatatt gcgcatgtgc acatcgagct gcggaccagg
     4621 cctgccgaat gcccagtcgt actccatctc catgggcatg aacggcgaga catggaaatc
     4681 cttgctgaag gcgaagcgat aaggcagctg tgccggttcg cgtgcgcagt ccagcacata
     4741 cgcgtgacgc tctttccagg gcgtgttggt aatctcggca acgatggctt cgaggcgctc
     4801 gccgtccggg gcgaagcaat agtagaaact caccggattg aagcaatggc cgaagtagcg
     4861 caaatgcgtg agcacgcgta tgggcccgcc cggacgcctg cccgtctcgc ggtgcacgag
     4921 gtcggcgatg gcggtcttca atgggaggct ggcgtctccg tagtagtctt cgcgacggaa
     4981 acaggcgaga ttcgcccgcc cgatggacca gaaccagcgg ccggcgaaca ccgtcggcaa
     5041 ctcgtcgagg tcgagataca tcatgaacag gcggtagcgg aacgcatggg ccacaggcag
     5101 gaagcgccga tggcgcaccc gtcccgtata gatggcgcta gccagcatgc gtggcttcct
     5161 cgaaatgctt taacgcggcc agggcgctga ccacgccatc ctcgtgaaag ccattgcgcc
     5221 agtaagcgcc gcaatacgcc gtgcgattgt ggccactgat ctcggcgtgg cgagcctgtg
     5281 ccgccgctcc ggcagtggtg tagaccggat ggtggtaggt caggcgcttg atgactttgg
     5341 ccgggttgat ctgatccgta tggttgagcg tcactagcag gggttgcttg gagcgcaggc
     5401 cctgcaggat attcatgttg taggtgacgg cgacccgatc cgacggggtt ggggtcacat
     5461 gatagttcca ggcggcccat gccagtttgc gccttggcat catgcgttcg tcatgatgca
     5521 ggaacacgtt gttctcctga taggggatgg cggcgaggat ctcgcgttcc tgcggtgagg
     5581 ggtcgtgcag catggccagc gcctggtcgc tgtggcatgc gaagaacacc cagtcgaagg
     5641 cttcttcgcc gccgtgcagc gaggtgacgc gcaccgaacg cttcaaccgg cgcaccttaa
     5701 gcacagggtt gttgagccgg atgttgctgc gaaacccctt gatcagttga tcgacatagc
     5761 ttgccgagcc gcccttgatc acgcgccatt gcgggcgatc gttgacggtc agcatgccat
     5821 gatggtggaa aaagcggaca aaaaagcggg ccgggaaatc cagcatgctt ttcgcgtccg
     5881 tcgaccaaat cgccgatccc atgggaacaa tgtagagatc gacgaagtct tggccgtaac
     5941 ctttcgtggc caggtaatct cccagccgga tctcgctgcc gttcgccagc agctcgagac
     6001 attcggtgtt gaatcgcagg atgtcgcgga tcatgcgata gaagcgtggc cgcagcaggt
     6061 ttcgccgctg cgcgaacaag gcattgagcg acgtgccgtt gtattccagg ccggtctttt
     6121 cgcaacgcac gctaaagctc atctcgcttg gctgtgaagc cacgcccagc gtatccagca
     6181 actggatgaa attggggtag gtgcggtcgt tgaagacgat gaaaccggta tcgacggcgt
     6241 ggtcttcgcc cagcaggccg atatggtgcg tgtgggtatg tccgccgaca tggttgccgg
     6301 cctcgaacac cgtgacgtca tgggcctgat gcaaatggta ggcggccgtg ttgcccgcga
     6361 tccctgaacc gatgatggcg atcttcatga tgttccgcgc ttatcccttg ttcgccgttg
     6421 tatcgcgcct gaccttgcgg gattcgcgga tctcctgcgg gacgccacgc aggttccaga
     6481 tcagccccat ggccgcgagc atgcgcagcc cgtagtaagt gaggtcaagc tcccaccagt
     6541 agaatccctg gcgcgccgag cccgggtagt ggtgatgatt gttatgccag ccttcgccca
     6601 gcgtgaatag ggcgatcagc cagttgttgc ggctatggtc cggcgtctcg taacgacgcc
     6661 cgccccagcc gtgggccagt gaattgacgc tgaaggtggc gtggtagagc acgaccgtgg
     6721 agatcacgaa gccccacacc agcagttgcc agccgttggt ccccaagcct ggcgccgctg
     6781 tttccagcca ggcgccaagg ccatagatgg cgagggcgaa cagcaacggt acgatcacat
     6841 cgaagcgatc gagcaggcgc aattcaggat atttcgcgag ttccttcacg cgctcggtgc
     6901 gcgtggcgaa atgcgcatcc gacagaaacc agccgagatg gctccagaag aagccgtgtc
     6961 ttaccggtga gtgcgcatcc tcctgacggt ctgaatgcgc gtgatggtgg cggtgcatcg
     7021 acgcccacca cagcggacca cgctgggcag ccgtggcgcc gaacagcgcg aatacgaatt
     7081 gtcccagccg cgaggtattg aacgccttgt gcgaaaaata gcggtgatag aaagcggtga
     7141 tggcgaacat gcgcaccgcg tagatcaccg cggcgaatac gaccgcgaat gcgctccagc
     7201 cgacccagat gactgcgaaa caggcgagat gcatgagcag gaaaggcaat acccgcagcc
     7261 agtcgacctt gttctcgacg gcgttgctct cgctcgctgc gccgctatcc agccagcgta
     7321 gcaagcgcaa caggaagggg cgcttgggct cggcaggcat catttgcgct ccggtgagag
     7381 tgtccagtcc tgcgggactt tgcgcagctt cgcggtgtcg tagccgagat cagccacgaa
     7441 cttgaccagg cgctggtaat cgtcgtcact gatcgagggt gtgcgcgcca tgatccagac
     7501 atagtcgcgg gcgttccgcg cgatcacggt ctgggtgtaa tccggattga gataggcgat
     7561 acggtattcc gccttgatcg gccagatgaa ttgcatgccc cacaaggcat tgccagtacc
     7621 cggctgcacg aaaccgtgcg ggttgtaacg cttgggcttg ccgtcgatgc tgccgctgcg
     7681 gaaggtgaac accgtgtcga tggtgccgtc ttcgagcagg cggtaggact cgaccgcgtt
     7741 gtaggcctcg gtctcgatcg ccgtcgggat gcaggcgatg acataccagt cacccatgaa
     7801 gcgttgcaga tcgacttgcg gcacggtggc gatcgccggc aggttctggc tggcgcagct
     7861 tgtcagcaag gtcgcgcagg cagccatcag ggcccggcgt aagcgtttca tgggagcgtg
     7921 tgtcttcatg gttcttgggt ccgtcatttc agcttgtaga tgatgtggta gccttccggc
     7981 gtcgtcgatt gcaggcccag ccactgctga tccttgccgt accagagatc gatcttcatc
     8041 ttggtcgcct ccagccgata gtgttcagcc tgcgtcttct ggccgcgtac ttcgatctct
     8101 tccatgccca gtgactggat gcgtacatcg agccattcgc cggtctgcgg gttgagcagt
     8161 ttgcgttgct tgagcatctg gggattccag taggcgaagg tcatcacgca ctgcggcaga
     8221 ctgagcgcgc ctttcgggcc gttgagcttg aacccgtcgt cctgcagcgc ccccttgacg
     8281 tcgctgatct ccttgttctc taccgtgcgc gattcgagcg atgtcaggca gtcgccatgc
     8341 cattgttcct gggcatcgtg gatgtagctg taggcctcga tgaacaggac cttgaccttg
     8401 aatctggcgg cgctgctgag cgtcttgtct tcgccttgcg tgtccagccg gaacacatgc
     8461 tgaccgatga ggcttttgtc gaggtagacc ttgaacgccc actcttgctc acgggcctgc
     8521 gacggcaggg cgcaaaataa aaggaataac gcaatgatca gacgcatcta tctgtcccta
     8581 gcttgttttt gcggaccggg gaagaacgcg ttggtcctgg cgatgtaagc ggcataggct
     8641 gggcggcgct cgccgatatc cttttccagc agggcgacgc cgctcacttt gagcagtagc
     8701 agcgtcatga gcagcgggcc aggcaggctc caccatgcgc cggcggcgac cgcgatcagg
     8761 taaaaccccc accagacaca gcattcgccg aaataattgg gatggcggct gtaagcccac
     8821 aggccctggt ccagcacctt gccgcgattc gcgctgttcg ccttgaactt cgccagctgc
     8881 cagtcagcga cgctctccca tgccaaaccg aacagcatga cgaccatgcc caccagatcc
     8941 agccagttga gttcgccact gctgtgaatg gcgccgagca gcggcagcga cacgatccag
     9001 gccagcaggg cctgcaggcc aaagatgatg tagaggctct tgagccagaa atacggttcg
     9061 ttattgcggc ggatgacgac atagcgatga tcctcgtgcg gcccccagtt acgcagggtg
     9121 agatagccac agagtcgcaa ggcccagagg tcgaccagca cgatcaccaa ggtcaggcgc
     9181 gcgccctcga accaggtgtg ccaggcgtag agcaatgccg ccagcaagaa gaaaaggctc
     9241 cacatgctat cgacatgggt cacgttgttg cgccacaggc tcagcagcca gcccagcacg
     9301 ccgaagacca tcatgcctgc caggccgcca agatagatcg accagtcaaa catgcagggc
     9361 tcctgctgtg agtctgcgcg cgcgcagcgt cgaggccagc ttgccgagga caggcatgag
     9421 cactgcccag ccgatggcca gggcgatcag caaggcgatc ggctgttgcc actggatcgc
     9481 gccaaggcgc gcgccgcccc agtaggccag tggaccgccg acgagaccga acaggatggc
     9541 gatcgcgggg cgtctttcca gccagcgcag gctgacattg agcgtggtgg caaacagcgc
     9601 ccagagggtc atcatccaga tcggtagcca acccgcttgc cagagattgg cgctgtaacg
     9661 caccaggtcg agcagcatca atgactggtc gaatgcgctg ccgatcaaca ccgtcagcag
     9721 cagcaggcgc agctccatac ctttatcggc ttgtcgggca agatgccagc cgatgatggg
     9781 cagggccact attggcccta gcagcgggtg gccgtaggcg gcgcccagca cgcaagcgaa
     9841 ccaccccagc tgaaacccga cgagattgac cagcatgtca gcgcggccgc tcgaacagat
     9901 aatggc
//
LOCUS       DATMTW010000319         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_229618, whole genome shotgun sequence.
ACCESSION   DATMTW010000319 DATMTW010000000
VERSION     DATMTW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229618"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VN084_06260"
     CDS             <1..806
                     /locus_tag="VN084_06260"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HWT29097.1"
                     /translation="DQIVALAQFLTGRQHAVLIEEKTAENSGIGGYTTVHVNAIGMHA
                     DLAIVLGGDGTMLTAARALVDHRIPLVGVNRGRFGFLTDLSSESMIEGLGHILDGAYD
                     TEQRILLSASITRQGKVISQGCALNDVVVNKNGVARLIELEVNIDGQFVHRQRADGLI
                     LATPTGTTAYSLSAGGPILHPTLDAIALVPICPHTLSNRPIAVHSASKVEVIVIHAED
                     AQVHFDGQMQLSLLEGDKVLVQCAENKVTLLHPEGHSHYGMLREKLHWG"
     gene            813..2465
                     /gene="recN"
                     /locus_tag="VN084_06265"
     CDS             813..2465
                     /gene="recN"
                     /locus_tag="VN084_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWT29098.1"
                     /translation="MLQSLSIRDFVIVDRLDLEFHDGFTVLTGETGAGKSILVDALSL
                     ALGARGEGGVTRAGCEKAEILASFDIRDNSGLRLWLTENEISVEDDTLLLRRVIHADG
                     RSRAFINGMASTVQQLRDAGEFLVDIYSQHAHHSLLKQSYQRQTLDAYAGLSELAQQV
                     ADSYHYWFGLHKQRIEMEQNAAAYAEELAELRDQVRELQAISADAADWEALQQEHSRL
                     AHGASLLSGGEECRNLLSESDFSALRQLTQAQHKLQQLLDYDAALQEALDTLDSAIIQ
                     LEETDRFLNRYLNHADLDPERLAEIESRIQALHAAARKHRVRPEELVQLLADSQQRMS
                     ELEVLGNDGELARQEEAAKASYMRLAEELSAGRQHAADRLSRKISEEMQRLALAGGSF
                     VVELARQAPAAHGLEQVEFLVAGHAGVAPRPLSKVASGGELARISLAIRVVTAQQGDI
                     PTMIFDEVDVGIGGGVAEIVGQLLGQLGGKRQVLVITHLPQVAAQGAQHLKVSKTQAD
                     GQTLSRIHLLEQEARVEEVARMLGGVEITATTRKHAEEMLGY"
     gene            complement(2478..>2834)
                     /gene="fur"
                     /locus_tag="VN084_06270"
     CDS             complement(2478..>2834)
                     /gene="fur"
                     /locus_tag="VN084_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HWT29099.1"
                     /translation="LFENSKERHLSAEDVYKILLTSGEDVGLATVYRVLTQFEQAGLL
                     MRHHFESGKAVFELNQGGHHDHIVCLQCGRVEEFCDEEIEKRQKMAAEERGFKIHEHS
                     LYIYADCTKQPCPHKG"
BASE COUNT          538 a          844 c          905 g          547 t
ORIGIN      
        1 gggaccagat cgttgccctg gcccagttcc ttacgggtcg ccagcatgcc gtcctgatcg
       61 aggagaagac ggcggagaac tcgggtatcg gcggttacac caccgtccat gtcaatgcca
      121 tcggcatgca tgccgatctc gccatcgtgc tcggtggcga cggcaccatg ctgaccgcgg
      181 cccgtgcgct cgtcgatcac cggatcccgt tggtcggcgt caatcgcggc aggttcggtt
      241 ttctcaccga cttgagttca gagtccatga tcgaaggcct gggccacatt ctcgatggtg
      301 cgtacgatac tgaacagcgc atcctgctct ccgccagcat cacccgtcag ggtaaagtca
      361 tcagccaggg ttgtgcgctc aacgacgtag tcgtcaacaa gaatggcgtc gcccgcctga
      421 tcgagctgga agtgaacatc gacggtcagt tcgtccatcg gcagcgcgct gatggcctga
      481 tcctggcgac ccccaccggc accacggcgt attcgctgtc cgccggcggg ccgatcctgc
      541 atccgacgct ggatgcgatc gcgctggtgc cgatctgtcc gcacaccctc agcaaccgcc
      601 cgatcgcggt gcacagcgcg agtaaggtcg aagtcatcgt catccatgcg gaagatgccc
      661 aggtgcattt cgatggtcag atgcagcttt ccctgctcga aggcgacaag gtattggtgc
      721 aatgcgccga gaacaaggtc accttgctgc atcccgaagg tcacagccac tacggcatgc
      781 tgcgcgaaaa attgcactgg ggctgaccgg gcgtgctgca aagtctatcc atccgcgatt
      841 ttgtcatcgt cgaccggctc gaccttgagt tccatgatgg cttcaccgta ttgactggcg
      901 agaccggcgc cggcaaatcc atcctggtcg atgccttgtc gctggcgctc ggtgcgcgcg
      961 gcgagggcgg cgtcacccgc gctggttgcg agaaggcgga gatactcgcc agtttcgata
     1021 tccgcgacaa ctcgggcttg cgtctgtggt tgaccgagaa cgagatatcc gtcgaggacg
     1081 ataccctgtt gctgcgacgc gtcatccatg ccgatggccg ttcacgcgct ttcatcaatg
     1141 gcatggcctc caccgtgcag caactgcgcg atgccggtga gttcctggtc gatatctaca
     1201 gccagcacgc gcatcattcc ttgctcaagc agagctatca gcggcagacg ctggacgcct
     1261 atgccggctt gagcgagctg gcgcaacaag ttgccgatag ctatcactac tggttcggct
     1321 tgcacaagca gcgtatcgaa atggagcaga atgctgcggc ttatgccgag gagctggccg
     1381 agttgcgcga ccaggtgcgg gagctgcaag cgatctctgc tgatgccgcg gactgggagg
     1441 ccttgcagca ggaacacagt cgtcttgccc atggcgccag cctgttaagc ggtggcgagg
     1501 aatgccgtaa cttgctgagc gaaagcgatt tttcagcact acgccagctc acgcaggccc
     1561 agcacaagct gcagcaactg ctggattacg atgccgcgtt gcaggaggcc ctggatacgc
     1621 tggactccgc catcatccag ctggaagaga ccgaccgttt cctcaaccgt tacctgaatc
     1681 atgccgatct cgacccggaa cggctggctg agatcgagag ccgcatccag gctttacatg
     1741 cggcggcgcg caagcatcgt gtgcggccgg aagaattggt gcaactgctc gcggacagcc
     1801 agcagcgcat gtcggaactc gaagtgctgg gtaacgatgg cgaactcgca cggcaggaag
     1861 aggcggccaa ggcgagctat atgcggttgg cggaagagct cagtgcaggc cgtcagcatg
     1921 cggctgaccg gctctcgcgg aagatcagcg aggagatgca gcgactggcg cttgccggtg
     1981 gcagtttcgt cgtcgagttg gcgcgtcagg cacctgccgc ccatgggctg gaacaggtgg
     2041 agttcctggt cgcggggcac gcgggggtcg ctccccgccc cttgagcaag gtcgcttccg
     2101 gcggcgagct agcccgcatc agtctggcga tccgcgtggt caccgcgcaa caaggcgata
     2161 tcccgaccat gatctttgat gaagtcgatg tcggtatcgg cggcggcgtg gccgagatcg
     2221 tcggtcagct actcggccag ctcggcggca agcgccaggt gctggtcatc acccatctgc
     2281 cgcaggtggc ggcccagggc gcgcagcatc ttaaggtgag caagacgcag gccgacggtc
     2341 agaccttgag tcgcatccat ctactggagc aggaggcgcg cgtggaagag gtggctcgca
     2401 tgctgggcgg ggtggagatc acggcaacga cgcgcaagca tgccgaggag atgctggggt
     2461 actgaggccg agggcgctta gcccttgtgc gggcaaggtt gcttggtgca gtcggcgtag
     2521 atgtagagcg aatgttcgtg gatcttgaat ccgcgctctt cggcagccat cttctgacgt
     2581 ttttcgatct cttcgtcaca gaactcctcg acgcggccgc attgcaagca gacgatgtgg
     2641 tcgtgatgcc cgccctggtt cagttcgaat acggctttgc cgctctcgaa atgatggcgc
     2701 atgagcaagc cggcctgctc gaactgggtc agtacgcgat agaccgtcgc caagccgaca
     2761 tcctcgccgg aagtgagcag gatcttgtag acatcctcgg ccgacaggtg acgttccttg
     2821 ctgttctcga acag
//
LOCUS       DATMTW010000320         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_464149, whole genome shotgun sequence.
ACCESSION   DATMTW010000320 DATMTW010000000
VERSION     DATMTW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464149"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /gene="obgE"
                     /locus_tag="VN084_06275"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             <1..232
                     /gene="obgE"
                     /locus_tag="VN084_06275"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899545.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWT29100.1"
                     /translation="DMVEDPKQVAKDFVKAYGWDGPCFAISALGGIGTKELTYAIMEH
                     LEATKRLEAETLEQSRIDSSNTEQSRNEFPTT"
     gene            213..1331
                     /gene="proB"
                     /locus_tag="VN084_06280"
     CDS             213..1331
                     /gene="proB"
                     /locus_tag="VN084_06280"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWT29101.1"
                     /translation="MSSLLLDARCLVVKVGSSLVTNNGEGLDQTAIAAWADQISTLVK
                     QGRQVVLVSSGAVAEGMQRLGWKKRPTAVNELQAAAAVGQMGLVQMYERCFASHGLHT
                     AQVLLTHDDLADRKRYLNARSTLRTLLDMKVIPIINENDTVVTDEIRFGDNDTLGSLV
                     ANLIEADALVILTDQSGLYSADPRKNPTAEFIQRAIAGDVALEAMAGGAGSTVGTGGM
                     LTKILAAKRAARSGAHTVIASGREPNLLARLAQGEVIGTHLKAEQIKTIAKKQWLADH
                     LRLSGQVVLDDGAIKALRQDGKSLLPIGVVSVEGHFERGEVVACVDTGGHEIARGLVN
                     YSAAETARILRKPSSEIEKILGYVDESELIHRDNMILL"
     gene            1404..1904
                     /gene="purE"
                     /locus_tag="VN084_06285"
     CDS             1404..1904
                     /gene="purE"
                     /locus_tag="VN084_06285"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWT29102.1"
                     /translation="MTQEKPVVAIIMGSDSDWPVMKAAAQMLADFGISYQAQVVSAHR
                     TPDLMFKFAESAAANGFKVIIAGAGGAAHLPGMVAAKTTLPVLGVPVPSKHLQGQDSL
                     LSIVQMPKGIPVATFAIGEAGAANAGLFAASMLANNDSALAEKLAQFRARQTDKVLSM
                     ELADKP"
     gene            1938..3077
                     /locus_tag="VN084_06290"
     CDS             1938..3077
                     /locus_tag="VN084_06290"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWT29103.1"
                     /translation="MLSKHTILPPAMLGMLGGGQLGRFFVIAAHELGYKVTVLDPDKH
                     SPAGKIADVHLCAAFDDQAALAEMARTCEAVTTEFENVPAATLEFLAQSRTVRPSASA
                     VAIAQHRISEKTFFRDAGLPVGQFVVVNQETDLPADDSALYPAILKVARFGYDGKGQA
                     RVKSRAEALAAFATFKQEPCVLEQMLPLDYEVSVVLARDAQGKIAAFPVAENSHLNGI
                     LDVSIAPARGSDVIKAKAQALAIEIAGKLDYVGVLGVEFFVSEGKLLVNEMAPRPHNS
                     GHYTIDACVTDQFEQQVRALTGLPLGDASLHSQAVMVNILGDVWKDGEPAWQKALAHT
                     ALKLHLYGKHEARPGRKMGHFTVLGQDRDTVVNQALIARAELGIG"
BASE COUNT          646 a          942 c         1021 g          582 t
ORIGIN      
        1 cgacatggtg gaagacccga agcaggtcgc gaaagacttc gtcaaggcat acggctggga
       61 tggtccctgt tttgccattt cggcactggg cggcatcggc accaaagagc tgacctatgc
      121 catcatggaa catctggaag ccacgaaaag actggaagcg gaaaccttgg agcaatccag
      181 gatagactca tccaacacag aacaatcacg caatgagttc cctactactt gatgcacgct
      241 gcctggtcgt gaaggtcggc tccagcctcg tcaccaataa cggtgagggg ctggatcaaa
      301 ctgcgatcgc cgcctgggcc gaccagatct cgacgctggt caagcagggc cggcaagtgg
      361 tgctggtctc cagcggtgcc gtcgccgaag gcatgcaacg gctgggctgg aagaagcgtc
      421 cgaccgccgt caacgaactg caggccgccg ccgccgttgg ccagatgggt ctggtgcaga
      481 tgtatgagcg ctgttttgcc agccatgggc tgcacacggc gcaggtgctt ctgacccatg
      541 atgacctggc cgaccgcaag cgttatctca atgcccgcag tacgctacgc accttgctgg
      601 acatgaaggt catccctatc atcaacgaga acgataccgt ggtgacggac gagatccgct
      661 tcggcgacaa cgatacgctg ggctcgctgg tggctaacct gatcgaggcc gatgcgctgg
      721 tcatcctcac cgaccagtcc ggactctatt cggccgatcc gcgcaagaac ccgacagcag
      781 aattcatcca gcgcgccatc gccggcgacg tcgccctgga ggcgatggcg ggcggcgccg
      841 gtagtaccgt cggcaccggc ggcatgttga ccaagatcct cgccgccaaa cgcgcggccc
      901 gcagtggcgc gcataccgtg atcgcctcag gccgcgagcc gaatttgctg gctaggctgg
      961 cccagggcga agtcatcggc acgcatctga aagccgagca gatcaagacc atcgccaaga
     1021 agcaatggct ggctgaccat ttgcgcctct caggtcaggt ggtgctggat gacggtgcca
     1081 tcaaggcgct gcgccaggat ggcaagagcc tgctaccgat cggtgtggtg tcggtggaag
     1141 gccattttga gcgtggcgag gtcgtcgcct gcgtcgatac cggcggccat gagatcgcgc
     1201 gcggcctcgt gaattacagt gcggcggaaa cagcgcggat cttgcgcaag ccgagtagcg
     1261 agattgaaaa gatactgggt tatgtcgatg aatcggaatt gatccatcgg gacaatatga
     1321 ttttgcttta acggattcat gtcgcgctgc aggcggttga gcacgcgata ccggaatctt
     1381 gtttcgcttt aaaggggtag ctaatgacac aagaaaaacc agtggtcgcc atcatcatgg
     1441 ggtccgacag cgattggcca gtgatgaaag cggctgccca gatgctggcg gatttcggca
     1501 tcagctatca ggcgcaggtc gtgtccgcgc accgtacgcc cgatctcatg ttcaagttcg
     1561 cggaaagtgc cgccgctaac ggcttcaagg tcatcatcgc cggtgccggc ggcgcagcgc
     1621 atctgccggg catggtcgcg gcaaagacca ctttgccggt gctgggcgtg ccagtacctt
     1681 ccaaacacct gcaggggcag gattccctgc tctccatcgt gcagatgccc aagggcatcc
     1741 cggtcgccac gttcgccatc ggcgaagccg gcgccgccaa tgccggcctg ttcgcggcgt
     1801 ccatgctggc caataacgat agcgcgctgg cggagaagct ggcgcagttc cgcgcccggc
     1861 agacagacaa ggtcttatcc atggaattgg ccgacaagcc ctgagcgctt gccagccatg
     1921 tttgaaatta cggagttatc ttgagcaagc acaccattct tcccccggcc atgctgggga
     1981 tgctcggcgg cggccaactt ggtcgctttt tcgtgatcgc agcccatgag ctgggttaca
     2041 aggtgaccgt cctcgatccg gacaagcaca gcccggcggg caagatcgcc gacgtccacc
     2101 tgtgcgccgc tttcgacgat caggcggccc tggctgagat ggccaggacg tgtgaagccg
     2161 tgacgaccga gttcgagaac gtgccggcag ccacgcttga gttcctggca caaagccgta
     2221 ccgtgcgtcc gagcgcttcg gcggtggcga tcgcgcaaca tcgcatttcc gaaaagacct
     2281 tcttccgtga cgcgggcctg ccagtcggcc agttcgttgt cgtcaatcag gaaacggatc
     2341 tgccggcgga cgatagcgcg ctctacccgg cgatcctcaa ggtggcgcgt ttcggttatg
     2401 acggcaaggg ccaggcacgc gtgaaaagcc gcgcagaagc attggcagca ttcgcgacct
     2461 tcaagcagga gccttgcgtg ctggagcaga tgctgccgct ggattacgaa gtctcggtcg
     2521 tgctggcgcg tgacgcgcaa ggcaagatcg ccgcgttccc ggtggcggaa aacagccacc
     2581 tgaatggcat cctcgatgtc agtatcgcgc cggcgcgcgg ttcggacgtg atcaaggcca
     2641 aggcgcaggc gctggcgatc gagatcgccg ggaaactgga ttatgtcggc gtgcttggcg
     2701 tcgagttctt cgtcagcgaa ggcaaattgc tggtcaacga gatggccccg cggccgcata
     2761 actccggcca ttacaccatc gacgcctgcg tcaccgacca gttcgagcag caagtgcgtg
     2821 ccttgaccgg tttgccgctg ggcgacgcca gcttgcacag ccaggcggtg atggtgaata
     2881 tcctgggcga cgtctggaag gacggcgagc ccgcctggca gaaggcgctc gcgcatacgg
     2941 cgttaaagct gcacctctac ggtaagcacg aggcgcggcc aggacgcaag atgggacact
     3001 tcacggtgtt gggccaggac cgcgatacgg tcgtgaacca ggccttgatc gcgcgcgcag
     3061 agctaggcat cggttagggc ggggctgcct gcgccattca gacgcttgct ctctgggtgg
     3121 gtccgggatg ggaccttagc gcctagaaaa tctatcctgg cctaggaatt cgtggcgcca
     3181 gaaatcggag a
//
LOCUS       DATMTW010000321         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_329133, whole genome shotgun sequence.
ACCESSION   DATMTW010000321 DATMTW010000000
VERSION     DATMTW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329133"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..277)
                     /gene="pqqA"
                     /locus_tag="VN084_06295"
     CDS             complement(206..277)
                     /gene="pqqA"
                     /locus_tag="VN084_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HWT29104.1"
                     /translation="MWTKPAATEMRFGFEVTMYVCNR"
     gene            495..989
                     /locus_tag="VN084_06300"
     CDS             495..989
                     /locus_tag="VN084_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HWT29105.1"
                     /translation="MLVRVCKIFLIQCIGLFALVVAYNNVVDYQTNFAFVQHVLSMDT
                     ISPDAQLKTRAITLPYLHHAVYVSIILGEFVTALLCLLGSVQLVRKVRAGALVFNKAK
                     AMAIAGLVNGFILWFFGFMTVGAEWFLMWQSEDWNGQEAAFRFIVCIGIVLIAVLHDD
                     KPLI"
     gene            1009..3078
                     /locus_tag="VN084_06305"
     CDS             1009..3078
                     /locus_tag="VN084_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HWT29106.1"
                     /translation="MNKHLKTFLWILVAVLGASAVGGIALNRGESINALWFVTAAVCV
                     YAIAYRFYAAWIAAKVLVLDPTRATPAERLNNGRDFIPTNKWIVFGHHFAAIAGPGPL
                     VGPTLAAQFGYLPGTLWILIGAVLGGAVQDMVTLFYSTRRNGRSLGQMARDELGPVGG
                     AAALIGTFAIMIILIAVLGLVVVNAMKHSPWATSTVAATIPIAMIVGLYMRNFRVGKV
                     LEASALGVILLLLAVAAGGWIDHQPALRVLFDHEGLSLAWVVIAYGFAAAILPVWLLL
                     APRDYLSTFMKLGTVILLAIAIIILQPEVKMPALTQFIDGSGPIFGGKLFPFVFITIA
                     CGAISGFHALISSGTTPKLLENERDIRMIGYGGMLLESFVAIMAMIAATVLEPGVFFA
                     INSPAGVVGKEAVDAVATINGWGFAVTVEQMQQLAHAMGESTLFARTGGAPSLAVGMA
                     SIFGSAFGEHLLALWYHFAIMFEAIFILTTLDAGTRVGRFMLQDMLGNLWPRMAETSW
                     YPSVLISSALVVAGWGYFLYIGVIDPNGGVNILWPLFGISNQMLAAIALSVATGILVK
                     SGKLRFAWVTGLPLAWLVLITTSAAWEKMFSTDVRVGFFAAANDMAQKLASGALSPEK
                     AAVAPQLIFNQHLDAWLTMFFVVVLWVVVLDMLRVCYRFLARKPVPPLTETAHIPSRL
                     VEDWVRD"
     gene            3078..3257
                     /locus_tag="VN084_06310"
     CDS             3078..3257
                     /locus_tag="VN084_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HWT29107.1"
                     /translation="MLRRLKRLWQAVRRLSGDDAYERYLQQFAEHGHEGEPLSREAFF
                     KQWQDGKWKGVKRCC"
     gene            complement(3284..3541)
                     /gene="infA"
                     /locus_tag="VN084_06315"
     CDS             complement(3284..3541)
                     /gene="infA"
                     /locus_tag="VN084_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWT29108.1"
                     /translation="MAKEELIEMNGQVMEVLPDSRFRVTLENGHELVAYTGGKMRKNH
                     IRILAGDKVTLELSPYDINNGRIVFRHIESRGPAPAPRRRY"
BASE COUNT          620 a         1071 c         1107 g          816 t
ORIGIN      
        1 agaaaagatg cgggagatgg taccgtccta tggtcagtcg ctagtgcagg atgctgactt
       61 gaccgccgtg gtgcggcaac ggacattgaa gacattgaag ctgggatgag atcccgggcc
      121 aggaaatgaa aaacgggagt ctgagctccc gttttttgca ttcagcgttc gaattaacga
      181 tgacgctgtc gattcacgca caaccttagc ggttgcagac gtacatcgtg acttcgaagc
      241 caaaacgcat ttcagtagca gctggtttgg tccacatgat gcatctcctt aaagttctat
      301 ggttaaaatg atgttgtaat gctatgactg cattatgcgc gccaggtttg ccgacagcac
      361 tgcacgcatt catgaatgcg gcctcatgat tttcatgaat ccgcatgtgc ggatggcgtc
      421 atgcctgcct gcatccgccc tgccgggctt gcggagggcg ggggcacaac aataacgatg
      481 atagggagag aagtatgctg gtgagagtct gcaagatatt cctgatccaa tgtatcggcc
      541 tgttcgcgct ggtcgtcgcc tataacaacg tggtcgatta ccagaccaac ttcgccttcg
      601 tgcagcacgt gttgtcgatg gatacgatct ctcctgacgc acagctgaaa acacgggcca
      661 tcacgctgcc ctatctgcat cacgcggtct atgtctccat tatcctcggt gaattcgtca
      721 ccgccttgct ctgcctgcta ggctccgtgc agctggtgcg caaggtgcgc gccggtgcgc
      781 tggtgttcaa caaggccaag gcgatggcca tcgctggcct cgtcaacggc ttcatcctgt
      841 ggttcttcgg cttcatgacg gtcggtgccg aatggttcct gatgtggcag tcggaggact
      901 ggaacggtca ggaagcggcc ttccgtttta tcgtttgtat cggtatcgtg ctgattgccg
      961 tgctacatga cgataaaccc ctgatctgat tttgctgtcc tcctccttat gaataaacat
     1021 ctcaagactt tcctgtggat actggtggct gtcctcggcg ccagcgcggt cggcggcatc
     1081 gcgctcaatc gcggcgagag catcaatgcc ttgtggttcg tcaccgccgc cgtctgcgtc
     1141 tatgccatcg cctaccgttt ctatgccgcc tggatcgcgg ccaaggtgct ggtgctggat
     1201 ccgacccggg cgacgcccgc cgagcgcctg aacaacggcc gcgactttat cccgaccaat
     1261 aaatggatcg tcttcggcca tcatttcgcc gcgatcgctg gcccggggcc gctggtcggc
     1321 ccgacgctgg cggcgcaatt cggttacctg ccaggcacgc tctggatcct gatcggcgcc
     1381 gtgctcggcg gcgcggtgca ggatatggtc accctgttct attccacccg gcgcaacggc
     1441 cgcagcctgg gccagatggc gcgcgatgag ctgggaccgg tcggtggcgc tgcggccctg
     1501 atcggcacct tcgccatcat gatcatcctc atcgccgtgc tcggtctggt ggtggtcaat
     1561 gccatgaaac acagcccgtg ggcgacctcc accgtcgcag cgaccattcc catcgccatg
     1621 atcgttggcc tgtatatgcg taacttccgc gtcggcaagg tgctcgaagc ttccgcgctg
     1681 ggcgtcatcc tgctcttgct ggcggtcgcc gcgggcggct ggatcgacca tcagccggca
     1741 ctgcgcgtgc tgttcgacca cgaaggcctg tcgctggcct gggtcgtcat cgcctacggc
     1801 ttcgccgccg ccatcctgcc ggtgtggctg ttgctggcgc cgcgcgacta cctgtcgact
     1861 ttcatgaagc tgggcacggt gatcctgctc gccatcgcca tcatcatcct gcaacccgag
     1921 gtgaagatgc cggcactgac gcagttcatc gatggcagtg gccccatctt cggcggcaag
     1981 ctcttcccct tcgtcttcat caccatcgct tgcggcgcca tctcgggctt tcacgcactc
     2041 atctcgtccg gcaccacgcc caagttgctg gagaacgagc gcgatatccg catgatcggt
     2101 tacggcggca tgctgctcga atccttcgtc gccatcatgg ccatgatcgc cgccaccgtg
     2161 ctcgagcctg gcgtgttctt cgccatcaac agcccggccg gtgtcgtcgg caaggaggcc
     2221 gtcgatgcgg tagcgaccat caatggctgg ggcttcgcgg tcacggtcga gcagatgcag
     2281 cagctggcgc atgccatggg cgaatcgacg ctgttcgccc gcactggcgg ggcgccctcg
     2341 ctggcggtag gcatggccag catcttcggt agcgcgttcg gtgaacatct tctggccttg
     2401 tggtaccact tcgccatcat gttcgaggcg atcttcatcc tgaccacgct cgatgccggc
     2461 acgcgcgtcg gccggttcat gctgcaggac atgctgggta atctatggcc gcgcatggcc
     2521 gagacgtctt ggtatccgtc ggtgttgatc agcagtgcgc tggtggtggc aggctggggc
     2581 tatttcctct acatcggcgt catcgacccg aacggcggcg tcaatatcct gtggccgttg
     2641 ttcggcatct ccaaccagat gctggcggcg atcgcgttgt cggtggcgac cggtatcctc
     2701 gtcaaatccg gcaagctgcg cttcgcctgg gtgacagggc tgccgctggc ctggctggtg
     2761 ctgatcacga cctcagctgc ctgggagaag atgttcagca cggatgtgcg tgtcggcttc
     2821 ttcgccgccg ccaacgacat ggcgcagaag ctggccagtg gcgccttatc gccggagaag
     2881 gccgcagtcg cgccacagct tatcttcaac cagcatctgg atgcctggct gacgatgttc
     2941 ttcgtagtcg tattgtgggt ggtggtgctc gatatgctac gggtttgcta tcggttcctc
     3001 gccagaaagc cggtgccacc cctgaccgaa acggcgcaca tcccgagccg gctggtggaa
     3061 gactgggtgc gcgactgatg ctgcggcgat tgaaacgctt gtggcaggcg gttcgccgcc
     3121 tgtcgggaga cgatgcctac gaacgctatc tgcagcaatt cgctgagcat ggtcatgaag
     3181 gcgagccgct gtcacgcgag gccttcttca agcagtggca ggacggaaaa tggaagggtg
     3241 tgaagcgttg ctgctaaagc gggagctcag ttcaaagcta ggctcagtag cgacggcgcg
     3301 gagctggtgc cgggccacgg ctctcgatat gacggaacac gattcggccg ttgttgatgt
     3361 cgtagggcga gagttccagg gtcaccttgt cgccggcgag gatgcggatg tggttcttgc
     3421 gcatcttgcc gccggtgtag gcgaccagtt cgtgaccatt ctcgagggtg acgcggaaac
     3481 gggaatccgg cagcacctcc atgacctggc cgttcatttc aatcaattct tcttttgcca
     3541 tcgccggctc tcctttagat gattcaagtt gctgcctgtt tggcaggtgt cgggctacgc
     3601 cctgagcaat cggg
//
LOCUS       DATMTW010000322         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_195845, whole genome shotgun sequence.
ACCESSION   DATMTW010000322 DATMTW010000000
VERSION     DATMTW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195845"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..708
                     /locus_tag="VN084_06320"
     CDS             184..708
                     /locus_tag="VN084_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWT29109.1"
                     /translation="MSLYLDRMSEWDSSLCIRVNRTSHNLWVRQWFRLISRLGDGIFW
                     YTVMLGIVLTQGDAGVVPAIHMGLAGLTGTLVYKWLKGKTLRPRPYEVHQDIWLTGRP
                     LDRFSFPSGHTLHAVAFCSVGLTYYPELTWLLLPFTVMVGMSRVILGLHYPSDVVAGA
                     VIGGAIAALSFLLL"
     gene            complement(884..1726)
                     /locus_tag="VN084_06325"
     CDS             complement(884..1726)
                     /locus_tag="VN084_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HWT29110.1"
                     /translation="MAKRHLALLCWLLPLVLHTGEAHAWGLVTHLYFAHSLLWAMPLL
                     DPRLQRAIKRFPELVMAGACLPDLAIVSPSFRDTHDWEYARRMLQAAKTDEETAIAVG
                     YASHLYVDVIAHNHFVPAHEAMWLDNDTMLTHIASEWAMDAHLSPLLDTSPGRLLRSH
                     QALLVAFISPLFRCSRRRTLKAVRALAFWDQVLRTVKLPHIIYRSARLVDKRVGKNFI
                     YYIAKTQSALAEIGIVLEGVRPSWEPEISDMDSDSLAAWRQVCVTHLAALHPTPIRYF
                     DKAS"
BASE COUNT          336 a          522 c          524 g          372 t
ORIGIN      
        1 gtggtgaaaa aagccgcagc ctgatccttt caggcggcat ccctgccgcc tgaaatttct
       61 ccttaagatt catcaatctg tcatataggc gtgccatcct gcacgcatga aaacaccatt
      121 catccatagc aaaggttcct acccggagcc tacaaacaag gtggtctcgc tatgcctcac
      181 aagatgagtc tttacctgga tcgcatgagc gaatgggaca gttcgctttg catccgtgtc
      241 aatcgcacca gccataacct atgggtgcgc cagtggttcc gcctcatcag ccggctgggg
      301 gatggcatct tctggtatac cgtcatgctc ggtatcgtgc tcacccaagg cgatgccggt
      361 gtcgtgcccg cgatccacat gggtctggcc ggcctcaccg gtacgctggt ctacaagtgg
      421 ctcaagggca agaccctgcg cccgcgcccg tatgaagtgc atcaggatat ctggctcacg
      481 ggccgcccgc tcgaccgctt cagtttccct tccggacaca cgctacacgc cgtcgcattc
      541 tgtagcgtag gcctcaccta ctatccggag ctgacctggt tgctgctgcc gttcacggtg
      601 atggtcggca tgtcgcgcgt gatcctgggc ctgcattacc cgagtgatgt ggtggctggc
      661 gccgtcatcg gtggcgccat cgccgcgctg agtttcctgt tgctatgaat atcctgacct
      721 gaatcgtttg ctgtactgat cggcgctgct ctgtctgaac ggcccgatcg ctccccagcc
      781 tcaccggacc tgtgtccggt ttttttatgc gcatgttcgc gcgctagcag gttcttgcgc
      841 cgggtctttt acactgggtt ttcttgcgct aggacttcct aagctaagac gccttgtcga
      901 aatagcggat cggcgtggga tgcaaggcag caagatgcgt cacgcagacc tgccgccagg
      961 cggcgaggct atcgctgtcc atatcgctga tctccggctc ccaggatggc ctcacgccct
     1021 caagcacgat gccgatctcg gccagcgccg attgggtctt ggcgatgtaa tagatgaaat
     1081 tcttgccgac acgcttatcc accaggcgcg cactgcggta gatgatatgc ggcagcttca
     1141 ccgtgcgcag tacctgatcc cagaaggcga gcgcgcgcac agccttgagc gtacgacgtc
     1201 tcgagcagcg gaacagcggc gagatgaatg cgaccagcaa cgcctgatgg ctgcgcagca
     1261 agcgcccggg tgaggtatcc agcagtggcg acaggtgggc atccatcgcc cactccgagg
     1321 cgatatgtgt gagcatggtg tcgttatcca gccacatcgc ttcatgcgcg ggcacgaagt
     1381 ggttatgggc gatgacatcg acatacagat ggctcgcgta gccgacggcg attgccgtct
     1441 cttcgtcggt cttggcggct tgcaacatgc gccgggcata ttcccaatcg tgcgtgtcgc
     1501 ggaacgatgg cgagacgatg gcgagatcgg gcaggcaggc acctgccatg accagctcgg
     1561 ggaaacgctt gatggcgcgc tgtagccgcg ggtcgagcaa gggcatggcc caaagcaggg
     1621 aatgcgcgaa ataaaggtgg gtgacgaggc cccaggcatg ggcttcgcct gtgtgcaaca
     1681 ccagcggcag aagccagcac agcaatgcga gatgtcgttt tgccatgctg cgcaatttgc
     1741 cgccacagcg tgta
//
LOCUS       DATMTW010000323         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_364429, whole genome shotgun sequence.
ACCESSION   DATMTW010000323 DATMTW010000000
VERSION     DATMTW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364429"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VN084_06330"
     CDS             <1..539
                     /locus_tag="VN084_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29111.1"
                     /translation="WYVGNCHKWLFAPKGCAFLWAAPGSRDFLQPPVVSLRMEEGFPA
                     TFDWVGTRDPSAWLSISAAIAFYREMGGEAIPAYLHAKVLAAADLICAAWQVELPAKR
                     EDYGAMVTLPFPFEGEATLERANQLRDQLWQAHRIEVPLFAFGGRLWLRISAQLYNVI
                     DDYVALAAAVKRLTSHRA"
     gene            complement(626..1429)
                     /locus_tag="VN084_06335"
     CDS             complement(626..1429)
                     /locus_tag="VN084_06335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWT29112.1"
                     /translation="MKVTTGALLMLLVSGLSQPALADNAVATVNGKPIKQSIYDYIVK
                     DATARGQKVDDNVREIIVNKLISSELVYQEAQRVGLDKQPDFQAKEELARRELLVNAY
                     LQDYIKKNPISEADTKAAYEQYKKELGDKEYSARHILVATEAEAKDLIAQLNKGADFA
                     KLAKEKSKDPGSKEKGGDLGWFSLGGMVKPFSDAVAKLQKGGISTEPVQTQFGWHVIK
                     LEDTRTTQAPAYDKVKDGLQKQLQQRQLEKLLTDLRTKAKIVDNTAAKK"
     gene            complement(1450..1719)
                     /locus_tag="VN084_06340"
     CDS             complement(1450..1719)
                     /locus_tag="VN084_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWT29113.1"
                     /translation="MTLHDEIRERLTSALQPARLELRDDSALHAGHAGNRGGGHFHVV
                     IVSSQFCGKSQIMRHRAIYQALSGLMPDRIHALSIQAIASDEPSP"
     gene            complement(1743..2075)
                     /locus_tag="VN084_06345"
     CDS             complement(1743..2075)
                     /locus_tag="VN084_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWT29114.1"
                     /translation="MWYAIVAEDHPDSLEKRMAARPQHLARLQALQDEARLLLAGPFP
                     AIDSEDPGVAGYSGSLIIAAFNNLASAQAWAQADPFVTAGVYAKVTVKPFRKTLPAMP
                     VLPDIGLD"
     gene            complement(2075..>2455)
                     /locus_tag="VN084_06350"
     CDS             complement(2075..>2455)
                     /locus_tag="VN084_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HWT29115.1"
                     /translation="SGVIITVFGGATLLLHDESFIKWKPTILYWFFSAGLLLSPLLFK
                     RNLIRSLMEKQISMPEPIWARLNLAWATFFAALGVLNLYVAFNFSTDTWVDFKLFGTM
                     GLMFAFIIGQTLLLNRYITEKDPE"
BASE COUNT          424 a          691 c          789 g          551 t
ORIGIN      
        1 actggtatgt cggcaattgc cataaatggt tgttcgcccc caaaggttgc gcgttcctct
       61 gggcggcgcc gggttcgcgg gatttcctgc agccgcccgt ggtgtcgttg cgcatggagg
      121 aaggcttccc tgccaccttc gattgggtcg gcacacgcga ccccagcgcc tggctctcaa
      181 tctctgccgc catcgctttc tatcgcgaga tgggtggcga ggccatcccc gcctacctgc
      241 acgccaaggt cctggcggcc gcggatctga tatgcgcggc ctggcaggtc gaattgccgg
      301 ccaagcgtga agactatggc gcgatggtga ccttgccatt tccattcgaa ggtgaggcga
      361 ccttggagcg tgccaaccag ctgcgtgacc agctttggca agcgcaccga atcgaggtgc
      421 cgctgttcgc gttcggtggg cgcctctggc tgcgcatctc ggcacaactg tataacgtga
      481 tcgacgatta cgtcgcgctg gcggcagcgg tgaagcgact gacaagccat cgggcctgac
      541 gtggacaaca aaaaagcccg cagatgcggg ctttttcaat caaaccgagg attcaatcaa
      601 accgaggcgt gatcctcagc gcagcttatt tcttggctgc cgtattgtcg acgatcttgg
      661 ctttcgtgcg cagatcggtc agcagcttct ccagctgacg ttgctgcaat tgcttctgca
      721 aaccgtcctt gaccttgtcg taggccgggg cttgcgtggt gcgggtgtct tccagcttga
      781 tcacgtgcca gccgaactgg gtctgtaccg gttcggtgga gatgccgccc ttttgtagct
      841 tggcgactgc gtcgctaaaa ggcttgacca tgccgccgag tgagaaccag ccgaggtcac
      901 cgcctttttc cttggagccc gggtccttgg atttctcctt ggccagcttg gcgaaatcgg
      961 cgcccttgtt cagttgcgcg atcaggtcct tggcttcggc ttcagtggcg accaggatat
     1021 ggcgtgcgct gtattccttg tcgccgagtt ccttcttgta ttgctcgtag gcggccttgg
     1081 tatcggcttc gctgatcggg ttcttcttta tgtaatcctg caggtaggcg ttgaccagca
     1141 gttcgcggcg ggccagctct tccttggcct gaaagtccgg ctgtttgtcg aggccgacgc
     1201 gctgggcttc ctggtacacc agctcgctgc tgatcagctt gttgacgatg atctcgcgta
     1261 cgttgtcgtc gaccttctgg ccacgggcgg tcgcatcctt gacgatatag tcatagatcg
     1321 attgtttgat cggtttgccg ttgacggtgg cgacggcatt gtcggcgagc gccggttgcg
     1381 acagcccgga tacgagcagc atcagcaatg caccagtggt aactttcatg aagggtcctt
     1441 agggtcgggt tatggggagg gctcatcgct ggcgatggcc tggatactca atgcatggat
     1501 acggtcgggc atgaggccgc tgagcgcctg atagatggcg cgatgccgca ttatctgcga
     1561 ttttccgcaa aattgcgaac tgacgatcac gacatgaaaa tggccgccac cgcggttgcc
     1621 ggcatggcca gcgtgcagcg cgctgtcgtc gcgtaattca agtcgggccg gctgtagcgc
     1681 gctggtcagg cgctcgcgga tctcgtcgtg gagtgtcatg ggcaagtccc cggcagggct
     1741 cctcagtcga gcccgatatc cggcaatacg ggcatcgccg gtaaggtctt gcggaacggt
     1801 ttgacggtga ccttggcata gacgccggcg gtcacgaagg ggtcggcttg ggcccaggcc
     1861 tgtgccgaag ccagattgtt gaaggcggcg atgatgaggc tgccgctgta accggcaacg
     1921 cctgggtctt cgctgtcgat ggcagggaag gggccggcaa gcagcaggcg cgcctcgtcc
     1981 tgcagcgcct gtagccgtgc gagatgctgc ggacgggccg ccatgcgttt ttccaggcta
     2041 tccgggtggt cttcggcgac gatggcgtac cacatcattc agggtccttt tccgtgatgt
     2101 atctgttgag cagcagggtc tggccgatga tgaacgcgaa catcaggccc atggtgccga
     2161 acagcttgaa atccacccag gtgtcggttg agaagttgaa ggcgacatac aggttgagca
     2221 cgcccagtgc ggcgaagaag gtcgcccagg ccagattgag ccgcgcccag atcggttccg
     2281 gcatgctgat ctgtttctcc atcaggctgc ggatcaggtt gcgcttgaac agcagtgggc
     2341 tgagcagcaa gccagcgctg aagaaccagt agaggatcgt gggcttccac ttgatgaaac
     2401 tctcgtcatg cagcagcaac gtcgcgccgc cgaacacggt gatgatgacg ccgct
//
LOCUS       DATMTW010000324         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_396688, whole genome shotgun sequence.
ACCESSION   DATMTW010000324 DATMTW010000000
VERSION     DATMTW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396688"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1067
                     /gene="mch"
                     /locus_tag="VN084_06355"
     CDS             78..1067
                     /gene="mch"
                     /locus_tag="VN084_06355"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HWT29116.1"
                     /translation="MTETNQALDTRQWPSVNQLTNPLVKQLVDNARQYRVGVEQLPNG
                     TTIVDAGIRFPGGLEAGRLIAEICMGGLGHVSLQTSNVFPHWPWMLSVHSSNPILSCL
                     GSQYAGWSLAHEKFFSLGSGPGRALAGREELYKELGYRDSAEAACLVLESEKAPPMEV
                     IEKVSRDTGVKPENLTFILTPTRSLAGTVQIVARVLEVAMHKIHTLHFPLAHVVDGMA
                     SAPVPPPAPDFLIGMGRTNDAILFGGHAHVFVLGSDEAASQLARELPSSASRDYGRPF
                     AEVFKAVNMDFYKIDPMLFSPAAVTVTAVESGNSFVGGKLDAALLDQSFGYPA"
     gene            1051..1989
                     /locus_tag="VN084_06360"
     CDS             1051..1989
                     /locus_tag="VN084_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HWT29117.1"
                     /translation="MDILPESRRIPVFTDDPGWHGKALQRAFAARGHEAVFVSLKDCA
                     LDLSGSAPRILIPGFEAGLPAGAFVRGVPGGTLQQVIMRLDILHALSMLGVTVYNDGR
                     AIERTVDKAMTSLRLLLEKVPTPATWVCEARQQAQVVLSRETMAARPLVIKPVFGSQG
                     QGVRRLEPGMSLPVPMEAHVDGLYYLQQYVDSGEGAWHDYRVFVVAGEAVAAMKRYGS
                     QWINNVAQGGRCEAVATDDEVAQLAIAAAKAVAIDYCGVDIIRDRQGQLFVLEVNSIP
                     AWRGLQGVTHIDIAQLLVDDFIRRMQAGRRLSAVSA"
     gene            1986..>2540
                     /locus_tag="VN084_06365"
     CDS             1986..>2540
                     /locus_tag="VN084_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWT29118.1"
                     /translation="MSDAQRLALLFKQACLAEIEALKPGNVHIFADGHGMTVEDFIHS
                     AEAACVPIAASGRGVGERVRDAVEATWAEVGCNTNLGILLLCAPLLKAAEDRTGEGLL
                     SALRQVLARLTIADAEYAFRAIVRASPAGLGTAATEDVHAPAQVTLLQAMQVAQDRDR
                     IAMQYVSGFADVFTAAAFYEEVLAK"
BASE COUNT          458 a          769 c          823 g          490 t
ORIGIN      
        1 atgcggcaca gcgctgcctg ctcgaacgtg cgaacgaatt acaaaccctg ttcaatgcaa
       61 tggaaagcaa atagagaatg actgaaacca atcaagcact ggatacgcgc cagtggccta
      121 gcgtcaacca gttgaccaat ccgctggtga agcagctcgt ggataacgcg cggcagtatc
      181 gcgtcggcgt cgagcaactg cctaacggca ccaccatcgt cgatgctggt atccgcttcc
      241 ccggcggcct ggaagccggt cgcctgatcg ccgagatctg catgggcggt ctcggccacg
      301 tcagcctgca aacctccaat gttttcccgc actggccatg gatgttgtct gtccactcca
      361 gcaatccgat cctgtcctgc ctgggcagcc agtatgctgg ctggagcctg gcgcacgaaa
      421 agttcttttc gcttggctca ggcccgggcc gcgcgctggc gggccgtgaa gaactgtata
      481 aggaactggg ttatcgggac tcggccgagg cagcatgcct ggtgctggaa agcgagaaag
      541 ccccacccat ggaggtgatc gagaaggtgt cgcgtgacac gggcgtcaag cccgagaacc
      601 tgaccttcat cctgacgccg acccgcagcc tcgccggtac ggtgcagatc gtggcgcgtg
      661 tgctcgaagt ggcgatgcac aagattcata ccctgcattt cccgctggcc catgtggtcg
      721 acggcatggc cagcgcgcca gtgccgccac ccgcgccgga ttttcttatc ggcatgggcc
      781 gcaccaatga cgccatcctg tttggcggac atgcgcatgt gttcgtcctg ggcagcgacg
      841 aagccgcttc ccagctggcc cgcgagctgc cgagtagcgc ctcgcgcgat tacggcaggc
      901 cgttcgccga agtgttcaag gcggtcaaca tggattttta caagatcgac ccgatgctgt
      961 tcagcccggc tgccgtcacc gtgaccgcgg tcgaatccgg caacagtttc gtcggcggca
     1021 aactcgatgc agcactgctg gaccagtcct ttggatatcc tgcctgagtc gaggcgcatc
     1081 ccggtcttca ccgatgaccc gggctggcat ggtaaggcgt tgcagcgcgc ctttgctgcc
     1141 cggggccatg aagccgtttt tgtctcgctc aaggattgcg cgctcgacct ttcgggtagc
     1201 gcgccacgga tcctgatccc gggattcgag gcaggcctgc ctgcaggcgc gttcgtccgt
     1261 ggtgtgccag gcggcacctt gcaacaagtg atcatgcggc tcgatatcct gcatgcactg
     1321 agcatgctcg gcgtgaccgt ctacaacgat ggccgcgcga tcgagcgcac ggtggacaag
     1381 gccatgacca gcctgcggct cctgctggag aaggtgccga cacctgcgac ctgggtctgc
     1441 gaagcgcgtc agcaggcgca agtcgtcttg tcacgcgaaa ccatggctgc ccggccgctg
     1501 gtcatcaagc cagtgttcgg ctcacagggc cagggcgtca ggcggttgga accgggcatg
     1561 tcgctgccgg tgccgatgga agcccatgtc gatggcctct attacctgca gcaatacgtc
     1621 gacagtggcg agggcgcgtg gcatgactat cgcgtgttcg tcgtcgcggg ggaggcggtg
     1681 gccgccatga agcgttatgg cagccaatgg atcaataacg tggcccaggg aggccgttgc
     1741 gaggccgtgg ccaccgatga cgaggtggcc cagcttgcca tcgccgccgc gaaagccgtg
     1801 gcgatcgatt actgtggcgt cgatatcatc cgtgaccggc agggacaact gttcgtgctt
     1861 gaggtcaaca gcatcccggc ctggcgtggt ttgcagggcg ttacccatat cgatatcgcc
     1921 cagctgctgg tcgacgactt catccggcgc atgcaggctg gccgacgctt gtcggcggtg
     1981 tctgcatgag cgatgcgcag cgtctggcgc tgctgttcaa gcaggcttgt ctggcagaga
     2041 tcgaggcgct gaagccaggt aacgtccata tcttcgccga cgggcacggc atgaccgtcg
     2101 aggatttcat ccatagcgca gaagctgctt gtgtgccaat cgccgccagt gggcgcggtg
     2161 tcggcgagcg cgtgcgggac gccgtcgaag ccacctgggc tgaggtcggt tgcaacacca
     2221 acctcggtat cctgttgttg tgtgcgccgc tgctgaaagc cgcggaagac cgaacagggg
     2281 aagggttgct atcggcgttg aggcaagtgc tggcgaggtt gaccattgcg gacgccgagt
     2341 atgcgttccg tgccatcgtc cgcgcgtcgc ctgcgggtct tggcacggca gcgacggaag
     2401 acgtgcacgc gccagcccaa gtcaccctct tgcaggccat gcaggtggcg caggatcgtg
     2461 atcgcatcgc tatgcaatac gtcagcgggt tcgccgacgt cttcactgcc gcggcttttt
     2521 acgaggaggt tctagccaag
//
LOCUS       DATMTW010000325         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_396700, whole genome shotgun sequence.
ACCESSION   DATMTW010000325 DATMTW010000000
VERSION     DATMTW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396700"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1394)
                     /gene="gltA"
                     /locus_tag="VN084_06370"
     CDS             complement(99..1394)
                     /gene="gltA"
                     /locus_tag="VN084_06370"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWT29119.1"
                     /translation="MKKPTKVTLTFDDGSAPIELPVVSGNLGPNVIDIRQLGKHGVFT
                     YDPGFLSTASCGSEITFIDGDEGLLYYRGYPIQQLAEHCDFLEVSYLLMHGELPDVQE
                     KADFVSTINRHTMLHDQLNNIFRGFRRDAHPMAVMVGVVGSMSAFYNDSMDVRDPAVR
                     QVAAYRLLAKVPTVAAWSYKYNLGQPFMYPQKRYNFAQNFMHMMFATPTEDYEPNPVL
                     AKAFDSILILHADHEQNASTSTVRLAGSSGANPFACVAAGIASLWGPAHGGANEATLK
                     MLEEIGDVSRIGEYIKRAKDKSDSFRLMGFGHRVYKNMDPRAAIMRQTCHQVLDELGL
                     NDDPMFKLALKLEQIALEDDYFVSKQLYPNVDFYSGIVMRAMGIPNSMFTAIFALART
                     AGWVAQWNEMLSDPEARIGRPRQLYTGAKRRDFVPLEQR"
     gene            complement(1431..2525)
                     /locus_tag="VN084_06375"
     CDS             complement(1431..2525)
                     /locus_tag="VN084_06375"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWT29120.1"
                     /translation="MNGELFIGLMSGTSLDGVDAVVLGLSQPHHRILHTAFTPYPDAL
                     REQLLALHAPTHDELHQTALTSNQLAQLYADAVSQVLASSGLTPAQITAIGCHGQTVR
                     HRPEAGYTLQIGNAALLAELTGITVVSDFRSRDIAAGGQGAPLVPAFHAAVFGSPSHE
                     RAILNIGGIANITMLPRRDSIIGFDTGPGNLLMDAWAGLHLGQRYDANGEWAASGKVI
                     EDLLSRCLSDPFFDLPPPKSTGRDLFNMDWLTSKLRGDETPADVQRTLLELTAVSIAQ
                     AIRRCCPDVDALYVCGGGAHNRALLGSLHDKLPEVEVQLTDTLGIDADWVEAAAFAWL
                     ASQAMRRAPGNLPAVTGAKGERILGAVYAS"
     gene            complement(2522..>3036)
                     /locus_tag="VN084_06380"
     CDS             complement(2522..>3036)
                     /locus_tag="VN084_06380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWT29121.1"
                     /translation="KSFLRSPLEFTRISSGFSLGRFHPILQRMRAHKGVDYAAPTGTR
                     VKASADGVVDFVGKKGGYGNVIVLRHANGINTVYGHLSRFAAGLRRGQRVDQGEIIGF
                     VGMTGLATGPHLHYEFLIHGQHRDPLKVALPAAQPIKNKYLADFEANSAGLVAQINLL
                     SASNLASLEE"
BASE COUNT          642 a          846 c          935 g          613 t
ORIGIN      
        1 gggctgatat aagtctgatt gatctaagtc tgagccatgg atcgacgaca acattaaagg
       61 cgctgattgc gccttttttg ttgaccgtcg ccagcgcgct agcgttgctc tagcggcaca
      121 aaatcacggc gcttggcacc ggtatagagc tgccggggtc tgccgatcct ggcttcggga
      181 tcggacaaca tctcgttcca ctgggcgacc cagccagcag tgcgggccag tgcaaagatg
      241 gcggtgaaca tggaattggg aatacccatg gcacgcatca cgataccaga gtagaaatcg
      301 acgttcgggt agagttgctt gctgacgaag taatcatctt ccagcgcgat ctgttcgagc
      361 ttgagcgcga gcttgaacat gggatcgtcg ttcagcccca gttcatcgag tacctgatgg
      421 caggtctggc gcatgatggc ggcacgtgga tccatgttct tgtagacgcg gtggccgaag
      481 cccatgagcc ggaaggaatc gctcttgtcc ttggcgcgct tgatgtattc accgatacgg
      541 ctaacgtcgc cgatctcttc cagcatcttc agcgtggctt cgttggcgcc accgtgggcc
      601 ggaccccaga gcgaggcgat acctgcggcg acgcaggcga acggattggc accactggaa
      661 cctgctaggc gcacggtcga ggtcgaagcg ttctgttcgt ggtcggcgtg caggatcagg
      721 atactgtcga aggccttggc cagaaccggg ttgggttcat agtcttccgt cggtgtggcg
      781 aacatcatgt gcatgaagtt ctgcgcgaag ttatagcgct tttgcggata catgaacggt
      841 tgcccgagat tgtacttgta gctccaggcc gcgacggtcg gcaccttggc caatagccgg
      901 taagcggcga cctggcgcac agctgggtcg cgtacatcca tcgaatcgtt atagaaggcc
      961 gacatcgagc cgacgacgcc gaccatgacg gccatcggat gcgcgtcacg gcggaacccg
     1021 cggaagatgt tgttcagctg gtcgtgcagc atggtatgcc gattgatcgt gctgacgaaa
     1081 tcggcctttt cctgcacatc cggcagttcg ccatgcatca ggaggtacga gacctcgaga
     1141 aaatcacagt gctccgccag ctgctggatg ggatagccac gataatagag caggccctca
     1201 tcaccatcga taaaggtgat ctctgaaccg cacgaagcgg tcgacaggaa acccggatca
     1261 taggtaaaaa ccccatgctt tccgagttgg cgtatatcga tgacgttggg gccgagattg
     1321 ccggaaacga caggcaattc gatcggcgca gagccgtcat cgaatgtcag ggtgacttta
     1381 gtaggtttct tcataacggt atcgttggtt tcaaattgga atgcttcaaa tcagctagca
     1441 taaaccgcac ccagtattct ctcacctttc gcaccagtga cggcaggtaa gttgccgggc
     1501 gcgcgacgca tcgcctggct ggcaagccag gcaaaagcgg cggcttccac ccaatcggcg
     1561 tcgatgccca gcgtgtcggt cagctgcact tcgacctcag gcaacttgtc atgcaagctg
     1621 cccaacagtg cgcggttgtg cgcgccgcca ccgcagacat agagcgcgtc cacatctggg
     1681 cagcatcgac ggatggcttg agcgatactg accgcggtca gctccagcaa ggtgcgctgc
     1741 acgtctgccg gcgtttcatc tccccgcaac ttcgatgtca gccaatccat gttaaagaga
     1801 tcgcgcccgg tactttttgg cggtggcaga tcaaaaaagg gatcgctgag acatctggac
     1861 agcaagtcct cgattacctt gccgctggct gcccactcac cattcgcatc gtaacgctgg
     1921 cccaagtgca gaccggccca ggcgtccatc aagaggttgc ctggtcccgt atcgaagccg
     1981 atgatgctgt cccgcctcgg cagcatcgtg atattggcga tgccgccgat gttgaggatg
     2041 gcgcgctcgt gcgaggggga tccaaacact gcggcatgga aggctggaac aagcggtgca
     2101 ccttgcccgc cagcggcgat gtcgcgactg cggaaatcgc tgacgacggt gatgccagtg
     2161 agttcggcca gcaatgcagc attgccgatc tgcagcgtgt agccggcttc cgggcgatga
     2221 cgtacggtct ggccatgaca accgatggcg gtgatctgcg ctggcgtcaa acccgaagaa
     2281 gccagtacct ggctaacggc atcggcatag agctgggcaa gctggttgga ggtcagtgcc
     2341 gtctggtgca gttcgtcatg ggtgggcgca tgcaaggcga gcagctgctc gcgcagggcg
     2401 tccgggtaag gcgtaaaagc ggtatgcagg atccgatgat gcggctgcga caacccgagt
     2461 acgacagcat cgaccccatc caggctggtg cccgacatca agccgataaa cagctcacca
     2521 ttcactcttc aagtgacgca aggttgcttg cactgagcag attgatctgg gccaccagac
     2581 ctgcgctgtt ggcctcgaaa tcagcgaggt acttgttctt gatcggttgc gcagccggca
     2641 gggcgacttt cagtggatcg cgatgctggc cgtggatcag gaactcataa tgcaagtgcg
     2701 gccccgtggc caggccggtc atgccgacaa agccgatgat ctcgccctga tcgacgcgct
     2761 gacccctacg caggccggca gcgaagcggg agagatgacc gtatacggta ttgatgccat
     2821 tggcgtgacg caagacgatc acgttaccgt agccgccctt tttgccgacg aagtcgacga
     2881 ccccgtctgc cgatgccttg acccgggtgc cggtcggtgc ggcgtaatcg acacctttat
     2941 gagcgcgcat tctttgcagg atggggtgga agcgaccgag actgaaaccg gagctgatgc
     3001 gcgtgaattc cagtggagag cgcaggaaag acttat
//
LOCUS       DATMTW010000326         6552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_431149, whole genome shotgun sequence.
ACCESSION   DATMTW010000326 DATMTW010000000
VERSION     DATMTW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431149"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..950)
                     /gene="nikB"
                     /locus_tag="VN084_06385"
     CDS             complement(42..950)
                     /gene="nikB"
                     /locus_tag="VN084_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWT29122.1"
                     /translation="MARRLLGILPVVFGVLLLTFLLVHLVPGDPVEVMLGESASGADR
                     AQLRAELGLDQPVVVQFGHYLARLAQGDFGTSIHSGKAISSLLAERYPATLKLALLAL
                     CIALCIGLPLGIVAALKADRWPDHVATTLSLTLSAMPHFWLGPLLMLVFALWLGWLPV
                     SGMENGASILLPALTLGFGLAAILTRMTRASLLEVLNEDFVRTARAKGLDESTVILRH
                     ALRAALLPVVTVVGLQMGSLLAGTVITETVFAWDGIGRLLVESIEKRDYPVTQACVLV
                     IALSYVLVNLGTDLLYARLDPRVRLD"
     gene            complement(943..1422)
                     /locus_tag="VN084_06390"
     CDS             complement(943..1422)
                     /locus_tag="VN084_06390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWT29123.1"
                     /translation="MQIEISIAQQLLTVLDDDGELRAQYPVSTALKGTGEIKGSYQTP
                     RGRHVVRAKIGGDAPVNSVFVGRRPTGEIWTTMLGEQHPGRDWILTRILWLSGCEVGR
                     NRLGNVDSMQRYIYIHGTPDDQTIGEPHSHGCVRMRNDDVIALFDMVPVGTEVLING"
     gene            complement(1412..2860)
                     /locus_tag="VN084_06395"
     CDS             complement(1412..2860)
                     /locus_tag="VN084_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWT29124.1"
                     /translation="MLRVLVFLLSLTLAACAPDARDTDIRMALSQAPINLDPRYATDA
                     ASERVNRLLYRSLVDFDAHFKPAASLATWQQLDALHYRFKLGDSGRTFHDGSRLEATD
                     VAVTYNSLRTLRDSPHATEYANIREVQAVDADTVEFTLDAPDDQFPARLIIGILPARL
                     IAAKHDFSHQPLGSGPLRFVSWASALKLQRVSDGQGIVLSEVKDPTVRILKLMRGEVD
                     LLQGDLPPELVRYLRKQPHIAVATSPGANFSYLGFNTEDFALQNPLVRRAVAHAIDRQ
                     AIIDTAMVEGTRVAGAILPPEHWAGNPNLVSYDYDPKEARSLLERAGIQLPLKLVYKT
                     STDAQRVRLATILQAQMRPAGIELEIRSLDWGTFFDDIKQGKFELYGLTWVGIKTPEI
                     YRLAFHSQSIPPKGANRGRYRDPMLDALIDVQDWVGVTERVHQQLPYVPLWYEGQFAA
                     MREGLIHYPLRPDGNWDGLATIERQYANQHAD"
     gene            2894..3406
                     /locus_tag="VN084_06400"
     CDS             2894..3406
                     /locus_tag="VN084_06400"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HWT29125.1"
                     /translation="MSRIHSFPPVTSPDMRVLVLGSMPGEKSLQMQQYYAHPQNVFWK
                     IMGELAGASRELEYPQRIERLKQNGIGVWDVLASCHRPNSSLDSAITDMQVNDFATLF
                     LQHPLLTHVFFNGGKAEETYKRCVLKSLPETYGYLRYTRLPSTSPAHAGMPYLQKLDR
                     WKQLLGPLDN"
     gene            complement(3517..4875)
                     /gene="rlmD"
                     /locus_tag="VN084_06405"
     CDS             complement(3517..4875)
                     /gene="rlmD"
                     /locus_tag="VN084_06405"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HWT29126.1"
                     /translation="MKNSAPQAPLPIAHIESLDQEGRGVAHVEGKTIFIDGALPGEEV
                     TYKTHRNKKSYEVADVVDVLKPSIQRVKPQCKHFGYCGGCAMQHLEFSAQVAAKQRLL
                     ENDLWHIGRVHPETILPPIYGPAWGYRHKARLRVRNVPKKGGVLVGFNEKASSYVAHM
                     DSCEVLPKHLSDLIVPLQQLVGKLSVIDRLPQIEVAVGEHATVLVLRILEPLQPQDTP
                     HLEAFADEHGVQIWTQSKGPDTVKPFYPLDGAQLSYSLPEYGLTYPFKPTEFTQVNPH
                     INKVMLRRAMQLLSPQKGERIADFFCGLGNFTLPIARSGANVLGMEGSSVLVARAVES
                     ADLNQLASQVEFREADLFKMTPEALQGLGSFDKWLIDPPRDGALELIKSLPDAMNEAG
                     EPQVKWGPERIVYVSCNPATLARDAGVLVNVKGYRLKAAGVINMFPHTAHVESIALFE
                     KG"
     gene            5023..6105
                     /gene="aroG"
                     /locus_tag="VN084_06410"
     CDS             5023..6105
                     /gene="aroG"
                     /locus_tag="VN084_06410"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HWT29127.1"
                     /translation="MNQIQKFATDDVRIKEIKELRPPSELLDSLQASTSATENILATR
                     SAIHGILHSADDRLLVIIGPCSIHDYDAGVEYARKLLAERERLSRDLLIVMRVYFEKP
                     RTTVGWKGLINDPHLDGSFDINAGLALARKFLLEVNELGMPAGTEFLDTITPQYTAAL
                     VSWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNIRIAVDAIKAASSPHHFLSVTK
                     AGHSAIISTSGNEDCHVILRGGKTPNYDAAHVDKAAKELASGGLNAKVMIDCSHANSR
                     KEYKLQMEVAADVAGQLAHGDDRIMGVMVESHLNEGKQDHTPGCTLEYGKSITDPCLG
                     WEDTVKLLDTLANGVRARRQRDEEVE"
     gene            6114..6335
                     /locus_tag="VN084_06415"
     CDS             6114..6335
                     /locus_tag="VN084_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29128.1"
                     /translation="MKRLFYIDYPQESYEGQMHRYRCAFCKEETTKINGKLEGHLPTC
                     EYRIKMEQEGHDCAGKSVEPASHDTDDYD"
BASE COUNT         1367 a         2052 c         1960 g         1173 t
ORIGIN      
        1 cgcgatccag aacaacagga tgaggtaagg cagacgccgc atcagtcgag tctcactcgt
       61 ggatccagac gcgcatatag gaggtccgtg cccaggttga ccagcacata actgagcgcg
      121 atcaccagca cacaggcctg ggtcaccggg tagtcgcgtt tctcgatgga ttcgaccagc
      181 aagcgaccga tgccgtccca ggcgaacacg gtctcggtga tgacggtgcc ggcgagcagg
      241 ctccccatct gcaggccgac caccgtcacc acgggcagaa gcgccgcgcg caaggcatgg
      301 cggaggatca cggtcgactc atccaacccc ttggcccgcg cggtacgcac gaaatcctcg
      361 ttgagcactt ccagcaggct ggcgcgcgtc atgcgcgtga ggatggccgc aaggccaaac
      421 ccaagcgtca gcgcgggcag caggatcgac gcgccgttct ccatgccgct caccggtagc
      481 cagcccagcc acagggcgaa caccagcatg agcagaggcc caagccagaa atgcggcatg
      541 gccgagagcg tcaggctgag cgtggttgcc acatggtcag gccagcgatc cgccttgagt
      601 gcggcgacga tgcccagcgg cagcccgata cagagggcga tgcacaaagc cagcagggcc
      661 agcttcagcg tcgccgggta gcgttcggcc aacagcgacg agatcgcctt gccgctatgg
      721 atggatgtgc cgaaatcgcc ctgtgcgagc ctggccagat aatggccgaa ctgcacgacg
      781 acaggttgat ccagccccag ttcggcacgc agttgcgcgc ggtcggcgcc gctcgccgat
      841 tcgcccagca tcacctcaac cgggtcgccc ggcaccagat gcaccagcag gaaggtcagc
      901 agcaggacgc cgaaaacgac cggcaggata cccagcaacc gtctagccat tgatcagtac
      961 ttcagtcccg accggcacca tgtcgaacaa ggcgatcaca tcgtcattcc gcatgcgtac
     1021 gcagccatgg gaatggggtt cgccgatggt ctggtcgtcc ggcgtgccgt ggatatagat
     1081 gtaacgctgc atgctgtcga cattgccgag ccggttgcgg ccgacctcgc agcccgagag
     1141 ccagaggatg cgcgtcagga tccagtcgcg ccccggatgt tgttcaccga gcatggtggt
     1201 ccagatctcg ccggtgggac ggcggccgac gaagacgctg ttgaccgggg catcgccgcc
     1261 gatcttggca cgcacgacat gacgcccgcg cggcgtctgg tagctgccct tgatctcgcc
     1321 agtgcccttg agcgcggtcg aaaccgggta ttgcgcgcgc aattcgccgt cgtcatcgag
     1381 cacggtcaat aactgctgcg ctatggaaat ctcaatctgc atgctggttc gcgtactgtc
     1441 tttctatggt ggcgaggcca tcccaattgc cgtcgggcct caaagggtaa tgtatcaagc
     1501 cttcacgcat ggcggcaaac tggccctcgt accacaaggg cacatacggc aactgctgat
     1561 gcacgcgctc ggtcacgccg acccagtcct gcacgtcgat cagcgcatcc agcatggggt
     1621 cacgataacg cccgcggttg gcgcccttgg gcgggatgga ttgcgaatgg aaggccaggc
     1681 ggtagatctc cggcgtcttg atgccgaccc aggtcaggcc gtaaagctcg aacttgccct
     1741 gcttgatgtc atcgaaaaaa gtgccccaat ccaggctgcg gatctccagc tcgatgccgg
     1801 ccggccgcat ctgcgcttgc aggatggtcg ccagccgcac ccgttgggcg tcggtcgagg
     1861 tcttgtagac cagtttcagc ggcagctgga taccggcgcg ttccagcagc gaacgggctt
     1921 ccttggggtc gtagtcgtaa ctgaccaggt tagggttccc ggcccaatgc tcgggcggca
     1981 ggatcgcccc tgcgacacgg gtgccctcca ccatcgccgt gtcgatgatg gcctggcggt
     2041 cgatcgcatg cgccacggca cggcgcacca gcgggttctg taaggcaaaa tcctcggtgt
     2101 tgaacccaag gtaggagaaa ttggcgcccg ggctggtcgc caccgcgata tgcggctgtt
     2161 tgcgcaggta tctcaccagt tccggcggca ggtcgccctg cagcagatcg acctcgccgc
     2221 gcatgagctt gaggatgcgc acggtggggt ccttcacctc ggaaagcacg atcccctgcc
     2281 cgtccgacac ccgctgcagt ttgagggcgc tcgcccagct gacgaaccga agcggcccac
     2341 tacccaacgg ctggtgcgag aaatcatgct ttgccgcgat cagacgcgca ggcaggatgc
     2401 cgatgatgag ccgcgcaggg aactgatcat ccggcgcatc cagcgtgaat tccaccgtat
     2461 ccgcatccac tgcctgcact tcgcggatat tggcgtactc ggtggcatgc ggcgaatccc
     2521 gcagcgtgcg caaggagttg taggtcaccg cgacgtctgt cgcttccagt cggctgccgt
     2581 catggaacgt gcggccgctg tcgccaagct tgaagcggta atgcaaggca tccagctgtt
     2641 gccaggtggc aaggctggcc gccggtttga aatgcgcgtc gaaatccacc agcgaacggt
     2701 acagcaggcg attcacgcgc tccgaagcgg catccgtcgc atagcgcggg tcaaggttga
     2761 tgggggcttg cgagagggcc atgcggatgt cggtgtcgcg ggcatccggc gcgcaggcgg
     2821 ccagtgtcag cgacaacaag aaaacgagca ctctcagcat gtgcgcagtc tacttgagat
     2881 tagaatccat gacatgtccc gaatccacag tttcccgccc gtcacttcgc cggatatgcg
     2941 cgtattggtg ctgggctcga tgcccggcga gaagtccttg cagatgcagc agtattacgc
     3001 gcatccgcaa aacgtgttct ggaagatcat gggggaactc gcaggcgcca gccgcgagct
     3061 ggaatacccg caacgcattg aaagattgaa acaaaacggc atcggcgtgt gggatgtgct
     3121 cgcctcatgc catcgcccca acagcagcct ggattcggcc atcaccgata tgcaagtcaa
     3181 tgatttcgct acgctatttt tgcaacaccc attattgacg catgtgttct tcaacggcgg
     3241 gaaggcggag gaaacctaca agcgttgcgt gctgaaatcg ctgccggaaa cctacggcta
     3301 cctgagatat acgcgactgc cttcgaccag ccccgcacat gcagggatgc cttatctaca
     3361 aaaacttgat cgatggaaac aactgctagg gccgctagat aattgatttt gtgagatgca
     3421 atgcaaggcg ccaggagcac agcttccgca gacagtacat aaagtacggc aaggaagcgc
     3481 gcaccgcaca acgcagcagt gcgctcaaaa aatcgattat cccttctcga acagggcgat
     3541 agactcgacg tgtgccgtat gcggaaacat attgatcacg cccgccgcct tgaggcgata
     3601 gcctttcacg ttgaccagca caccggcatc gcgtgccagc gtcgccgggt tgcatgacac
     3661 atagacgatg cgctccggcc cccacttcac ctgcggctcg cccgcttcgt tcatcgcatc
     3721 tggtagcgat ttgatgagtt ccagcgcgcc gtcgcgcggc ggatcgatca gccatttgtc
     3781 gaagctgccg aggccttgca gggcttcagg cgtcatcttg aacaggtcgg cctcgcggaa
     3841 ctccacctgc gatgcaagct gattcaggtc ggcactttcc acggcgcgcg cgaccaacac
     3901 tgaggagcct tccatgccca gcacgttggc gccgctgcgg gcgatgggca gcgtgaagtt
     3961 acccaagccg cagaagaagt cggcgatgcg ctcgcccttt tgtggcgaca gcagttgcat
     4021 ggcgcgccgc agcatcacct tgttgatatg cgggttcacc tgcgtgaatt cggtcggctt
     4081 gaacgggtag gtcaggccat attctggcag cgagtaactg agctgcgcgc catccagcgg
     4141 gtagaacggc ttgaccgtgt ccggaccctt gctctgcgtc cagatctgca cgccgtgttc
     4201 gtcggcgaag gcttccagat gcggcgtatc ctgcggctgc aagggttcga ggatgcgtag
     4261 caccagcacc gtggcgtgtt cgcccaccgc cacttcgatc tgcggcagac ggtcgatcac
     4321 gctcaactta ccgaccagtt gctgtagcgg cacgatgagg tcggatagat gcttcggcag
     4381 cacctcgcag ctatccatgt gtgcgacata gctgctggcc ttttcgttga agcccaccag
     4441 cacgccgcct tttttcggca cattgcgcac tctcagacgc gctttgtggc gataacccca
     4501 ggccgggccg tagatcggcg gcaggatggt ctcaggatgc acgcggccga tatgccacag
     4561 atcgttctcc aatagccgtt gcttggccgc cacctgcgcg gagaactcca gatgctgcat
     4621 ggcgcagccg ccgcaataac cgaaatgctt gcattgcggc ttcacgcgct ggatcgacgg
     4681 cttgagcaca tcgaccacat cggcgacttc atagctcttc ttgttgcggt gggtcttgta
     4741 ggtgacttcc tcgcccggca acgcgccgtc gatgaagatg gtcttgccct cgacatgggc
     4801 gacgccgcgg ccttcctggt cgagcgattc gatatgggcg atgggcaatg gtgcttgtgg
     4861 tgctgaattt ttcatggccg cgattttaca ggacttttcc gccacctttc gtccccagcg
     4921 caacgggggc tcggacggca ggatgaaaaa acccgctgtg atccgggcca tccatagtaa
     4981 aatcagggac ttgaaacgtt gagacaacgg taatcccaag caatgaacca gattcaaaaa
     5041 ttcgccactg acgacgtccg catcaaggaa atcaaggagt tgcgtccgcc gtccgagctg
     5101 ctcgatagcc tgcaggccag cacgtccgcc accgagaaca tcctggcgac gcgttctgcc
     5161 atccacggca tcctgcatag cgcggacgat cgcctgctgg tcatcatcgg cccctgctcc
     5221 atccacgatt acgatgccgg cgtggaatac gcccgcaagc tgctggccga acgcgaacgc
     5281 ctgtcgcgcg acctgttgat cgtcatgcgc gtgtatttcg aaaagccgcg caccaccgtc
     5341 ggctggaaag gcctgatcaa cgacccgcac ctggacggca gtttcgatat caacgccggg
     5401 ctggcgcttg cccgcaagtt cctgctcgaa gtgaacgaac tcggcatgcc cgccggcacc
     5461 gagttcctcg ataccatcac gccgcaatac accgcagccc tcgtgagctg gggcgccatc
     5521 ggcgcgcgca ccaccgaatc gcaagtgcac cgtgagctgg cctccggcct ctcgtgcccg
     5581 gtcggcttca agaacggcac ggacggcaac atccgcatcg cggtcgatgc gatcaaggcc
     5641 gccagcagcc cgcaccattt cctgtcggtg accaaggccg ggcattccgc catcatctcc
     5701 accagcggca atgaagactg ccacgtcatc ctgcgcggcg gcaaaacgcc gaactacgat
     5761 gccgcgcatg tggacaaggc cgccaaggaa ctcgccagcg gcgggctcaa cgccaaggtg
     5821 atgatcgact gctcgcacgc caacagccgc aaggaataca agctgcagat ggaagtcgcc
     5881 gccgatgtcg ccgggcaact ggcgcatggc gatgaccgca tcatgggcgt catggtcgaa
     5941 tcgcacctca acgaaggcaa gcaggaccat acccccggct gcacgctgga atacggcaaa
     6001 tccatcaccg acccctgcct aggctgggaa gacaccgtaa aactgctgga caccctggcc
     6061 aatggcgtca gggcaagacg ccagcgcgac gaagaagtgg aatagaaagc accatgaaaa
     6121 gactgtttta tatcgactac ccgcaagagt cctacgaagg ccagatgcac cgttaccgct
     6181 gcgccttctg caaggaagag acgaccaaga tcaacggcaa gcttgaaggc cacctgccca
     6241 cctgcgaata ccgcatcaag atggaacagg aaggccacga ctgcgcaggc aaatcggtcg
     6301 agcccgcctc gcacgacacg gatgactatg attgagtgag cacgattgag cgactgtgat
     6361 cgagtaagca cggtcgagcg atcacgcact tagcacttcg tctcgacacc aaaaagcccg
     6421 gcaatgccgg gctttttcat gactgcaact gcatcactgg atggtcgtgg tcgtcatcca
     6481 gtcgatcatg gcctggtcgc cagggcgatc aacatgcacc ttgccgtctt ccagcgaggt
     6541 cacccagccg at
//
LOCUS       DATMTW010000327         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_28815, whole genome shotgun sequence.
ACCESSION   DATMTW010000327 DATMTW010000000
VERSION     DATMTW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28815"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VN084_06420"
     CDS             <1..573
                     /locus_tag="VN084_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HWT29129.1"
                     /translation="TRGFSMRPSMFGRLLRSEARSDSPRCSMPAADRTSVRALLTGFR
                     ADTSGVALVETALVAPFLAIILMGTVDAARYGAAKMKIQQAVNRGLEMSWMGGTDVVA
                     TDIQAQAAAQANVPTSAVTVTQTLECGGTATTWTTNANPCASGETARYTQIQISTTFT
                     PSFALGSLAKMLANSNGVVPISATGVLRIQ"
     gene            570..977
                     /locus_tag="VN084_06425"
     CDS             570..977
                     /locus_tag="VN084_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWT29130.1"
                     /translation="MTRFFRLLHQLACDCRGAGVLEFALVAPVLLGMLIGIPALGILF
                     YAQAGLRSTVEDAARYATTWPKPSEADIQARIASKQFGMNSANIVAPTVTFTTSSKPN
                     YVTITMGYNVTINFLLGSKTIALSETRRAYVTS"
     gene            994..>1865
                     /locus_tag="VN084_06430"
     CDS             994..>1865
                     /locus_tag="VN084_06430"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWT29131.1"
                     /translation="MPLLNCLSRLRTLWSDARGNALMLTALALPVMIGAAGLGVHTMQ
                     LSHVKRELQREADSAAMAGAYSLYQSQGNSAATAQANKALTQNALVPGATSTITPGAY
                     TTSGGTTYTSAMYVRLTSTQPTALMALLGMSTSTVAAEARAAVVSDGKVCFLAKESGN
                     NTGITFAGNSTLDLGCGAGTNSISSSAVNVNGQPTVKVSPIVAMGGITSSTAYAAGTM
                     LMPNHAALTDPFAGTNLDPSTNDVSNGKCKNGSNWNVISIASSDPPVNDISGSLYPPG
                     CYGTINVQGTLTLKP"
BASE COUNT          372 a          553 c          561 g          379 t
ORIGIN      
        1 acgaggggat tttcaatgcg tccttctatg tttggccgat tgttgcgcag cgaggcgagg
       61 agtgattcgc cgcgttgctc catgcctgcc gccgatcgga cgtctgtccg ggcgttgttg
      121 accggattca gggccgatac atcaggcgtc gcccttgttg aaacggcgct ggtcgcgccg
      181 ttcctggcca tcatcctcat gggcacggtc gatgcggccc gttacggcgc ggccaagatg
      241 aaaatccaac aagccgtcaa tcgcggtctg gaaatgtcat ggatgggcgg cacggacgtc
      301 gtcgcaaccg acattcaggc gcaggcggca gcgcaggcga atgtgccgac cagtgccgtg
      361 acagtcactc agacacttga gtgcgggggc acggcaacaa cctggaccac taatgccaat
      421 ccctgcgcct ccggcgaaac cgctcgctac acgcagattc agatttcaac gaccttcacg
      481 ccatcctttg cgctggggtc gctggcgaag atgctggcca actcgaatgg cgtcgtgccg
      541 atatccgcga cgggggttct gagaatccaa tgacacggtt ttttcgcctt cttcatcagc
      601 ttgcgtgcga ttgccgtggc gccggtgtgc tcgaattcgc gctggtagcg cctgtcctgc
      661 tgggcatgct gatcgggatc ccggcgctgg ggatactctt ctatgcgcag gctgggctca
      721 ggagcaccgt cgaggatgcc gcgcgttatg caacgacgtg gcccaagccg agtgaggccg
      781 acatacaggc aagaatagcg tcaaagcagt tcgggatgaa ctcggccaac atcgttgcgc
      841 cgaccgtcac gttcacgacc agttcgaagc ccaactatgt gaccataacg atgggctaca
      901 atgtcaccat caacttcctg cttggaagca agacgatcgc gctgtccgaa acgcgcagag
      961 cctatgtcac gagctaaagc cgaggagctg acgatgccat tgctcaattg cttgtcccgt
     1021 cttcgcacgc tttggtccga tgcgcggggc aatgcgctca tgctgacggc gctggcgctt
     1081 cccgtcatga tcggagcggc cgggcttggc gtccacacca tgcagctttc gcatgtgaag
     1141 cgggaattgc agcgggaggc cgactccgcg gcgatggcag gcgcctattc gctttatcag
     1201 agccaaggca atagcgcggc gacggctcag gcgaacaagg cgctgacaca gaatgctctt
     1261 gtcccgggag ccacgtctac gatcacgccg ggagcttata ccaccagtgg tggcaccact
     1321 tacacctcgg caatgtatgt ccgtttgacc agcactcagc ctacggcctt gatggccctg
     1381 cttggcatgt ccacgtccac cgtggctgct gaggcccggg cggcggtcgt atccgatggc
     1441 aaggtctgtt tccttgccaa ggagagtggc aacaacaccg ggatcacctt tgcgggcaac
     1501 tcgacactcg atctgggatg tggtgcgggc accaactcga tcagcagcag tgcggtcaac
     1561 gtgaacggcc agccgaccgt gaaggtttcg ccgatcgtag ctatgggtgg aatcacgtcg
     1621 tccaccgcct atgccgctgg caccatgctc atgcccaatc atgcagcgct gactgatccg
     1681 tttgcgggta ccaatcttga cccgagcacc aacgatgttt ccaatgggaa gtgcaagaac
     1741 ggcagcaatt ggaatgtcat aagcatcgca tcgagcgatc cgccggttaa tgatattagt
     1801 gggtctcttt atccgcctgg ctgctatgga acgatcaatg tccagggcac cctgacgctg
     1861 aagcc
//
LOCUS       DATMTW010000328         7109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_566085, whole genome shotgun sequence.
ACCESSION   DATMTW010000328 DATMTW010000000
VERSION     DATMTW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566085"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1191)
                     /locus_tag="VN084_06435"
     CDS             complement(277..1191)
                     /locus_tag="VN084_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HWT29132.1"
                     /translation="MRFLCLLCWIATLTIPGVSHAALAMQEVSPGIYVHQGVHEDLSE
                     GYHGDIANLSFVVGSKGVAVIDSGGSHQVGTQLRDAIRKVTSLPVLYVINTHVHPDHI
                     FGNTAFLEENPTYIGHAKLADAMERRRDSYMRNNASWMGEAFAGSAMVKPTLAVADKM
                     EIDLGGRRLQIQAYPIAHTNTDITVMDLDSATVWTGDLLFVDRTPSVDGDIKGWIEVI
                     DQLKELPAKRLIPGHGHIPEDGKAALEKEQHYLKVLLGDIRSSIKNGVVMEKAMDTAA
                     SSEKDKWLLFDIVNRRNVNNLYPALEWE"
     gene            1573..2172
                     /locus_tag="VN084_06440"
     CDS             1573..2172
                     /locus_tag="VN084_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWT29133.1"
                     /translation="MKNAVTRYTKTAVVLHWLIGIAIIGMLALGWFMSELPKEAPKQT
                     AYDLFNLGVYTWQLAEPASPRTFYFNLHKSIGITLLALILLRILWRLTHRPPALLASL
                     KPWERKLATAGHHLLYLLMIAMPVTGLLQALYSKYGVKWFGMPFLGGLDDKGMRDIFN
                     EAHEVIGFIFAAIIVVHILGALKHKFIDRDQTMSRMTLG"
     gene            complement(2259..3056)
                     /locus_tag="VN084_06445"
     CDS             complement(2259..3056)
                     /locus_tag="VN084_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HWT29134.1"
                     /translation="MSMLKYWVSSALLAATLLGMSQGAWAEADPKLWPVVKEAFFANR
                     TIEDADFMQITGPKRAESGAQVPITLSVDNSKGITIKKVYIIVDANPIQLAATYHLTD
                     LLGNLQLSTRIRMETDSYVRAIGEAADGKLYMANVVIRAAGGCGGTIENDEAAVRASA
                     GKIKMHVETPVKFGQATPTTFIIKHPMRTGLQRDLVSQGTIPAFYINKATFSFNDKEV
                     MAVDFGVGTSEDPYLRFDFVPSAPGVLEVKATDNEGKAFEHQIEVKS"
     gene            3228..3635
                     /locus_tag="VN084_06450"
     CDS             3228..3635
                     /locus_tag="VN084_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWT29135.1"
                     /translation="MSVQGFNHYNLRAPRDLLDTLRDFYCEVVGLSLGERPAFRSFGY
                     WLYAGGRDLLHLSECSPEEVRVTHVETTLDHVAFTCTDFAGTLARLERHGIAYGQREV
                     AATGIRQLFFKDPAGNGIEFNFAASEPAVDLKR"
     gene            complement(3632..4909)
                     /gene="glp"
                     /locus_tag="VN084_06455"
     CDS             complement(3632..4909)
                     /gene="glp"
                     /locus_tag="VN084_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWT29136.1"
                     /translation="MTTPLSDLVHNASCLDDYDPNAMPVAQARRFIRQYLSPVSTKTS
                     VPLRDSLGRVLAVDIHSPCNVPNHDNSAMDGYALNADDLQANGETRLKIVGTAYAGNA
                     YDGTLGQGDCVRIMTGAVMPAGCDTVVVQERVTVDGDHIRFNDAIKRGMNVRTAGDDL
                     KQGQVVLPRGHLMRAADLGLVASLGIGELQVYRKLCVAFFSTGDELASIGQPLAPGQV
                     YDSNRYTLYGMLTRLGVDVIDMGVVADDPVRLEQALMDASQCADVILTSGGVSVGEAD
                     FMKQLLEKLGQVLFWKIAMKPGRPLAYGKVGNAHYFGLPGNPVSVMVTFYQFVREALL
                     VLMGQPEPAATPLLRAVCTSDIRKLTGRTEFQRGVLYPDSDGQWKVKPTGSQSSGVLR
                     SMSEANCFIVLDETVGNIAAGATVSVQLLDGVI"
     gene            complement(4923..5522)
                     /gene="mobB"
                     /locus_tag="VN084_06460"
     CDS             complement(4923..5522)
                     /gene="mobB"
                     /locus_tag="VN084_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HWT29137.1"
                     /translation="MVGAGSIGFDEYISFPLPLLGVCAYGSDIGKTTLLTRLIPALAT
                     RGLRVSVIKHAHHRFDIDHPGKDSYRLREAGAVQTLIGSRHRWAVMTELARVPEAIRP
                     ETDLAILLARIEPAYADLVLIEGFKQAPIAKIEVHRPALGMPLLADHDPHIIAIAADA
                     AVITDRPVLDLDDPEAVADFVMQWLASQPTARLPVPISS"
     gene            complement(5501..6079)
                     /gene="mobA"
                     /locus_tag="VN084_06465"
     CDS             complement(5501..6079)
                     /gene="mobA"
                     /locus_tag="VN084_06465"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HWT29138.1"
                     /translation="MSISGVVLAGGKGRRMGGVDKGLVAFLGKPLIEHVVQRLRPQVD
                     EIFINANRELAQYGNLGYPVIEDDISGFAGPLAGLHKALQVSEHAYVLTVPCDSPFLP
                     HNLVERLMNGLVERDADLAVAKTGQQAHPVFCLCRKALLPNLEAYLQSGGRKFETWYS
                     TLEVAEVLFNDNAKAFANVNTPEELLWLEQAA"
     gene            complement(6323..>7109)
                     /gene="moaA"
                     /locus_tag="VN084_06470"
     CDS             complement(6323..>7109)
                     /gene="moaA"
                     /locus_tag="VN084_06470"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019008 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWT29139.1"
                     /translation="GEPLVRKNALKLFEEVGQLPGLRELVVTTNGSQLEHQAAALKRA
                     GVRRINISLDSLDAERFRKITRVGDLDKVLRGVAAARHAGFEGIKLNTVLMRGVNDDE
                     ALPLLRFAIEQEIDIAFIEEMPLGEVDHGRLDTYVSNEATLKQLQAHYPMVSSAETTG
                     GPARYWRIPGTRTKVGFISPHSHNFCESCNRVRITCKGELYLCLGQEDKVELMPLLRA
                     HPEDDQPLIAAIRDSMRIKPKGHDFDLQRAAPAVVRFMSHTGG"
BASE COUNT         1424 a         2037 c         2082 g         1566 t
ORIGIN      
        1 gtcttgtcct gcggcttctg atcgaatttg aaattgggca gctggctgag cgccgccaca
       61 tgcaggctga tagataaaac caatgcccag acaaacgtct ggtggcggtc ttcgcgagca
      121 cgggtggggg aattgaccaa ggctggactg cgcaggtgat gggttgaggt tcgtcgggta
      181 aaaaaccaaa gcgccgatta tacagtaagt ccttgcccag gctggtacca gcgcccgctt
      241 gctagcgaac gcctgtgccg ggtagccggc accgtctcat tcccactcaa gcgcgggata
      301 gaggttgttg acgttgcggc ggttgacgat atcgaacaac agccatttgt ccttttccga
      361 actggccgcc gtatccatgg ctttttccat gactacgcca ttcttgatgc tgctgcggat
      421 atcgccgagc aagaccttga ggtaatgctg ctccttctcc agcgcggcct taccgtcttc
      481 ggggatatga ccgtggccgg ggatcaggcg tttcgcaggt aattccttca gctggtcgat
      541 gacctcgatc caccccttga tgtcaccatc cactgagggt gtgcggtcaa cgaatagtag
      601 atcgccggtc caaaccgtgg cgctatcgag atccatcact gtaatgtcgg tattggtatg
      661 ggcgatgggg taggcctgga tctgcaggcg tctgccgcca agatcgatct ccatcttgtc
      721 tgctacggct agagtcggtt tgaccatcgc actgccggcg aaagcttcgc ccatccagct
      781 cgcgttattg cgcatgtaac tgtcgcgccg acgttccatg gcatcggcca gcttggcgtg
      841 gccgatatag gtcgggtttt cttcaaggaa ggcggtgttg ccaaaaatat ggtccggatg
      901 cacatgggtg ttgatgacat acaggacggg cagtgatgtc accttgcgga tggcatcgcg
      961 aagctgggtg ccgacctggt ggctgccgcc gctgtcgatg actgccacac ctttgctgcc
     1021 caccacgaaa ctcaggttgg cgatatcacc gtgatagcct tcggaaaggt cttcgtgcac
     1081 cccctgatgc acatagatac ccggagagac ctcctgcatg gcaagcgcgg cgtgtgacac
     1141 accgggtatg gtcagggtgg caatccagca aagcagacac aggaatcgca atcgggctct
     1201 cctctttgat acagttaggt cacaaggtac gtacgataaa taccacaccg ttagtcgcgt
     1261 ggcatcaagc gcacaccctg gttgctacta tgcggcatat tcggcgaaca ctcacgtcgc
     1321 catgctgccg caagcgtggc atgctgctga atgggacgct ggtatacaat gagcgcagtt
     1381 cgttccaggc attatgggcc gcataccgct tatgttcaat gcggttaggg ccactgcaac
     1441 gaaggttgaa ctagaacgct ctgtatacgt ctgaatagca ggtaaccgat gcatgacgca
     1501 tgttcttaca gtagagacct gcgacagtat gtgacgttcc ataaaaactc aattctaaat
     1561 agggaagaag cgatgaagaa cgctgttacg cgctatacca aaaccgccgt tgtgctgcac
     1621 tggctcatcg gcattgccat catcggcatg ttggcactgg gctggttcat gagtgaattg
     1681 cctaaagagg cccccaagca gactgcctac gatctgttca acctcggggt ctatacctgg
     1741 cagctggctg agccagcttc accacggacc ttctacttca atctgcacaa gtcgatcggt
     1801 atcaccttgc tggcactgat cctgctgcgt atcctatggc gccttacgca tcgcccgccc
     1861 gcgctgttgg cctcgctcaa gccctgggaa cgcaagcttg cgaccgcggg acatcatctg
     1921 ctgtatctgc tcatgatcgc gatgccggtg accggactgt tacaggcgct gtacagcaag
     1981 tatggcgtca agtggttcgg tatgccgttc ctcggcgggc tcgacgacaa gggcatgcgg
     2041 gatatcttca atgaggcgca cgaggtgatc ggtttcatct tcgccgccat catcgtggtg
     2101 cacatcctgg gcgcgctcaa gcacaagttc atcgatcgcg accagaccat gagccgcatg
     2161 acgctgggtt aaacaccctg ccaaggcaat aaaaaagggg gcatagcccc cttttttatt
     2221 gatgctgtcg tcaaggatgt tgtcggctgc cgtcgtgctc agcttttcac ttcgatctgg
     2281 tgctcgaagg ccttgccttc gttatcggtc gctttcactt ccagtacgcc aggtgcgctg
     2341 ggtacgaaat cgaaacgcag atagggatct tcgctggtgc ccaccccgaa atccaccgcc
     2401 atgacttcct tgtcgttgaa gctgaaggtc gccttgttga tgtagaaggc ggggatcgta
     2461 ccctgtgaga ccagatcacg ctgcaaacca gtgcgcatcg ggtgcttgat gataaaggtc
     2521 gtgggcgtgg cctggccgaa tttgaccggc gtctccacgt gcatcttgat cttgcctgcg
     2581 ctggcgcgta ccgcggcctc atcgttctcg atcgtgccgc cacagccgcc ggccgcacgg
     2641 atcacgacat tcgccatgta gagcttgcca tccgccgctt cgccgatcgc gcgcacatag
     2701 gaatcggtct ccatacggat acgggtggac agttgcaggt tacccaaaag atcggtgagg
     2761 tgataagtcg cggccagctg gatggggttt gcgtcgacga tgatgtatac cttcttgatc
     2821 gtgatcccct tgctgttatc gacagacagc gtgattggca cctgtgcccc gctttctgcc
     2881 cgcttgggtc ctgtgatctg catgaaatcg gcatcttcga tagtgcgatt ggcgaagaac
     2941 gcttccttga ccacgggcca cagcttcgga tccgcttctg cccatgcccc ctggctcatg
     3001 cctagcaaag tcgctgcgag cagcgcggac gacacccagt acttcaacat gctcatctcc
     3061 tcaagttctt gttatattgt ggattacacg ctctaagacg cctagtataa aagcaagttc
     3121 agattgccgc ctttttgtct tcacgtatca tcgatcatca tggacgcgct gtctgtgacc
     3181 atgatcaccg aacgcaatct ccttttaacc acaagcaaag ggaaaccgtg tctgttcagg
     3241 gcttcaatca ctacaatcta cgcgcaccga gggatttgct cgacactttg cgcgattttt
     3301 attgcgaggt cgtcggcctg tcgctcggcg aacgacctgc ctttcgtagc ttcggctatt
     3361 ggctctatgc cggtggccgc gacctgctac acctgagtga gtgctcacca gaggaagtgc
     3421 gcgtgaccca tgtcgagacc acgctcgacc atgtggcttt cacctgcacc gattttgcgg
     3481 ggacgcttgc taggcttgag cgccatggca tcgcttatgg ccagcgtgaa gtagcggcga
     3541 ccggtatccg tcagttattc ttcaaggatc cggctggcaa tggcatcgag ttcaattttg
     3601 ccgcgagtga gcccgccgtc gatctcaagc gctagatgac gccatccagt agttgtacgg
     3661 acacggtagc cccggccgct atgttgccta ccgtttcgtc gagcacgata aaacagttgg
     3721 cctcggacat cgagcgtagc acccccgaac tctggctgcc ggtcggtttc accttccatt
     3781 gcccatcgct gtccggatag agtaccccac gctggaattc agtgcggccg gtgagcttgc
     3841 gtatatcaga cgtgcatacc gccctcagta gtggcgtggc cgctggttcc ggctggccca
     3901 tcaacaccag caaggcttca cgcacgaact ggtagaacgt caccatcacc gagaccgggt
     3961 taccaggcag accgaagtaa tgcgcgttgc cgactttgcc gtaggccagc ggtctaccgg
     4021 gcttcatggc gatcttccag aacaggacct ggcccagttt ttccagcaac tgcttcatga
     4081 aatccgcttc gcccaccgag acgccgccgc tggtgagtat gacatcggca cattgcgaag
     4141 cgtccatcag cgcctgttca agccgcaccg gatcgtcagc cacgacgccc atgtcgatga
     4201 cgtccacgcc aagtcgcgtc agcatgccat acagtgtgta gcggttactg tcatagacct
     4261 gacctggcgc cagaggctga ccgatgctgg caagctcgtc tccggtagag aagaaggcga
     4321 cacataactt gcgatagacc tgcagttcgc cgatgccgag cgaagcgacg aggccgagat
     4381 cggcggcgcg catgagatgg ccacgcggca ataccacctg cccctgcttc aggtcatccc
     4441 ctgctgtgcg tacgttcatc ccgcgcttga tggcatcgtt gaagcggata tggtcgccgt
     4501 cgaccgtgac gcgctcctgt actacgaccg tatcgcagcc tgcaggcatg acggccccgg
     4561 tcatgatgcg tacgcaatca ccttggccca aggtaccgtc gtatgcgttg ccggcgtagg
     4621 cagtaccgac gatcttgagc cgggtctcgc cgttcgcctg caggtcgtcg gcattgaggg
     4681 catagccgtc catagccgaa ttgtcgtggt tgggtacatt gcatggggag tggatatcca
     4741 ccgccagtac acggccaaga ctgtcgcgca agggtacgga tgttttggtc gataccggac
     4801 tcaagtattg gcggatgaag cggcgtgcct gtgccacggg catggcattg gggtcgtagt
     4861 catcaaggca gctggcgtta tgtacgaggt cggaaagtgg tgtggtcatg gtggtccatc
     4921 gatcatgaag atatgggaac aggtaggcgg gccgtgggtt ggctggcaag ccattgcatc
     4981 acgaaatcgg cgaccgcttc cgggtcgtca agatcgagca cggggcgatc agtgatgacg
     5041 gcagcgtcgg cggcgatggc gatgatatgc ggatcgtgat cggccaagag gggcatgccc
     5101 agagccgggc gatggacttc gatcttggcg atgggggctt gcttgaaacc ttctatgagg
     5161 accaggtcgg cgtaagccgg ctcgatgcgc gctaacagga tggccaggtc ggtctccggc
     5221 cggatagcct ccggcacgcg cgcgagctcg gtcatcaccg cccatcggtg gcgcgagccg
     5281 atcagcgtct gcaccgcccc tgcttcgcgc aggcggtagc tatccttgcc tggatggtcg
     5341 atatcgaagc ggtggtgcgc atgtttgatg accgatacgc gcaaaccgcg cgttgccagc
     5401 gcagggatca ggcgcgtgag cagggtggtc ttgccgatgt cgctgccgta agcacaaaca
     5461 ccgagcaggg gcagcggaaa agagatgtat tcgtcgaacc ctatgctgcc tgctccaacc
     5521 acaataattc ctcaggggtg ttcacgttgg cgaaggcctt ggcattgtcg ttgaacagca
     5581 cttcggctac ttccagcgtg ctgtaccagg tttcgaactt gcggccgcca ctttgcagat
     5641 aagcctcaag gttgggcagc agcgccttgc ggcacaggca gaataccgga tgcgcttgct
     5701 gaccggtctt ggcaacggcc agatcggcgt cacgctccac caggccgttc atcaggcgct
     5761 cgaccaggtt atgcggcaaa aagggcgagt cgcagggcac cgtcaataca taggcatgct
     5821 ccgagacttg caacgccttg tgcagacctg ccagggggcc ggcgaaaccg ctgatgtcat
     5881 cctcgatcac tggataacca agattgccat attgtgcgag ttcgcgattg gcattgatga
     5941 agatctcgtc gacctggggc cgcaagcgct gtaccacatg ctcgatcaga ggtttgccga
     6001 ggaacgcgac cagccccttg tcgacgccac ccatacggcg ccctttgccg cctgccagaa
     6061 ccacaccgct gatactcata atccatgaac cttattactg gcaacgcgca tcgcgcactt
     6121 gccgatcatt gaaaattctt gccgggccct gtctgagcga ccgatatgat gctgactcac
     6181 atcacgggta tgtcgatcac cttgaaagga gcgggcaagt gacgataaag ccctctatta
     6241 tccagcaaaa caagcgtctt gccgcatctg aacgctggcc gacggtgtct ggctggcctg
     6301 tcgcataccg gcaggcatgc gctcatccac ccgtgtgtga catgaagcgc acgaccgctg
     6361 gcgctgcccg ctgcagatcg aaatcgtggc ctttgggttt gatgcgcatg gagtcgcgta
     6421 tcgcagcgat caatggctgg tcatcttcgg ggtgtgcacg cagcaagggc atcagctcga
     6481 ctttgtcttc ctggcccagg cagaggtaga gctcgccttt gcaggtgatg cgcacacggt
     6541 tgcaggattc gcagaaatta tggctatgcg gcgagatgaa cccgaccttg gtgcgggtgc
     6601 cggggatgcg ccaatagcgt gccgggccgc cggtggtttc ggcgctgctg accatggggt
     6661 aatgtgcttg cagctgcttt agcgtggcct cattgctgac ataggtgtcg agccggccat
     6721 ggtcgacctc gcccagcggc atttcctcga tgaaggcgat atcaatctct tgttcgattg
     6781 caaagcgcaa tagtggtaac gcttcgtcat cgttgacgcc acgcatcagt acggtattga
     6841 gcttgatgcc ctcgaacccg gcatgcctcg ctgcagcgac tccgcgtaag accttgtcca
     6901 gatcgccgac ccgggtgatc tttcggaaac gctcggcgtc gaggctatcg aggctgatgt
     6961 tgatgcgacg aacgccggcc cgcttcagtg cggcggcttg atgctcgagc tgcgaaccgt
     7021 tggtggtgac gacaagctca cgcaggcctg gtagctggcc gacctcttcg aacagtttca
     7081 gggcattctt gcgcacgagc ggctcgcca
//
LOCUS       DATMTW010000329         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_196921, whole genome shotgun sequence.
ACCESSION   DATMTW010000329 DATMTW010000000
VERSION     DATMTW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196921"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VN084_06475"
     CDS             complement(<1..504)
                     /locus_tag="VN084_06475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWT29140.1"
                     /translation="MLKKRLLQWASEIRQRTDLHARLVLWDGQHFDLGRFNVPEVVIH
                     IKEMAAIPYLLGASMSKLGEAYVKGKIDVEGKLSDIIQLGFNLVRHTPSDSRKLQRLA
                     GYFTHTRRSDMQAIEFHYDLSNYFYQLWLDPNMVYSCAYFEHGDEDLATAQLKKIDHI
                     LTKLQLRP"
     gene            655..2196
                     /locus_tag="VN084_06480"
     CDS             655..2196
                     /locus_tag="VN084_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWT29141.1"
                     /translation="MAAVPPHALATRLDLHDYPATFPLARSLARRIHFHLGPTNSGKT
                     YEALQVLQRAESGVYLAPLRLLAMEVRDRLMEAGVPCNLVTGEERVLVPGARHTASTV
                     EMMNPARQVAVAVIDEIQMLQDESRGYAWTAALIGAPAKDVFVCGSEAVTAPCVSAIE
                     AMGEAYEIHRLERKTPLVLETTSLSGARYNRWRVKRHLQKGDAVIAFSRKDVLTLSAR
                     FRQWGFGVASIYGALSPEVRRTEARRFSGGEADILVATDAIGMGLNLPIRRVVFSATH
                     KFDGVAPRNLDATEVRQIAGRAGRYGIYPTGYVSAFEDEELLHIAQMLSTSDLSTMRK
                     LPITPNLMHMQVLSELQHNGRLGELLAYFSERVGIDSPIFETSTLNVHVAQGHLIDTH
                     APALSLRDKFTFSCAPVSLDKSHERDYFLICLKRYAAGQATALPAAPEWLSSTSPRHL
                     EAAENLGKDLSLYAWLSFKYPRIFYQGAEIPALRSRISRYIEAALLTQAGFGDTSREL
                     LYVYK"
     gene            complement(2272..2343)
                     /gene="pqqA"
                     /locus_tag="VN084_06485"
     CDS             complement(2272..2343)
                     /gene="pqqA"
                     /locus_tag="VN084_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HWT29142.1"
                     /translation="MWTKPAATEMRFGFEVTMYVCNR"
     gene            complement(2507..>2652)
                     /locus_tag="VN084_06490"
     CDS             complement(2507..>2652)
                     /locus_tag="VN084_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWT29143.1"
                     /translation="FGKGDLVTFPAGLKASWEVKQPLHKHYKLDGSVLCQIFRRIKLK
                     LGF"
BASE COUNT          515 a          779 c          802 g          556 t
ORIGIN      
        1 cggtcgcagc tgcagcttgg tcaggatgtg gtcgatcttt ttcagctgtg cggtcgccag
       61 atcttcgtca ccgtgctcga aataggcgca ggaatagacc atgttggggt cgagccagag
      121 ctggtagaaa tagttggata gatcgtagtg gaactcgatc gcctgcatgt cggagcgccg
      181 cgtatgcgtg aaataaccgg caaggcgttg cagttttcgc gaatccgacg gcgtatgccg
      241 taccaggttg aaaccgagct ggatgatgtc actgagcttg ccttcgacgt cgatcttgcc
      301 cttgacgtag gcctcgccga gtttgctcat gctggcgccg agcaggtagg ggatggcggc
      361 catctccttg atgtggatga ccacctctgg cacgttgaat cggccaaggt cgaaatgctg
      421 gccgtcccag agcaccagtc gcgcgtgcag gtcggtgcgc tgccggatct cggaagccca
      481 ctgcaagagt ctcttcttca acatgaatgc cactccttat cggttggagg caaggtcacg
      541 ccgggggaat catcatgcca gaaaataata tggaaatcat ggtcttgcaa aagttcccct
      601 ggcgcaagta gcgtatccgc tgcacgctcg gatgaaattt tgctacgctg cgccatggct
      661 gccgttcccc cgcatgcgct agcgacccga ctcgacctgc acgattatcc cgcgaccttt
      721 ccgcttgcac gttcgctcgc gcggcggatc catttccatc tggggccgac caattccggc
      781 aagacctatg aggccttgca ggtgctgcag cgcgcggaat ccggggtcta cctggcgccg
      841 ctacggctgc tggccatgga agtccgcgat cgcctgatgg aagccggagt gccctgcaat
      901 ctggtgacgg gcgaggagcg tgtgctggtg ccgggtgcgc ggcacacggc gagcacggtg
      961 gagatgatga acccggccag gcaagtcgcg gtggctgtca tcgacgagat ccagatgctg
     1021 caggacgaat cgcgcggcta tgcctggacc gctgccttga tcggcgcgcc agccaaagac
     1081 gtgttcgtct gcggttccga agcggtgacc gcaccctgcg taagtgcgat cgaagccatg
     1141 ggtgaggcat acgagatcca tcgactcgaa cgcaagacgc cgctggtgct tgagaccact
     1201 tcgctgagcg gcgcccgcta caaccgctgg cgcgtgaagc ggcatttgca gaagggtgat
     1261 gccgtcatcg ctttcagtcg caaggacgtg ctgacactga gcgcgcgttt caggcaatgg
     1321 ggattcggcg tcgccagtat ctacggggcg ctgtcgcccg aagtacgccg taccgaagcg
     1381 cgacgtttca gtggcggcga ggcggatatc ctggtggcca ccgacgccat cggcatggga
     1441 ctcaacctgc cgatccgtcg cgtggtgttc tctgccaccc ataagttcga cggcgtcgcg
     1501 ccgcgcaacc ttgacgcgac cgaagtgcgg cagatcgccg gccgtgccgg tcgctacggt
     1561 atctacccga cgggctatgt cagcgccttc gaggacgagg agctgctgca tatcgcccaa
     1621 atgttgagta cgtccgatct ctccaccatg cgcaaactgc cgatcacgcc caatctcatg
     1681 cacatgcagg tgctttccga actgcagcac aatggacgcc tgggtgagtt gctggcctat
     1741 ttttcagaac gtgtgggcat cgacagcccg atcttcgaaa cgtcgacgct gaatgtgcat
     1801 gtcgcgcaag gtcacctgat cgacacgcat gcgcccgcac tctcgttacg cgacaagttc
     1861 acgttctcct gcgcgccggt gtcgctcgac aagtcccacg aacgcgatta tttcctcatc
     1921 tgcctcaagc gctacgctgc gggacaggcg acggccctgc ctgcagcgcc ggaatggctg
     1981 tcatccacca gcccacgcca tctggaagcg gcagaaaacc tgggcaagga cttgagcctc
     2041 tatgcctggc tctcgttcaa gtatccgcgg atcttctatc agggggcgga gatccccgcc
     2101 ctgcgcagtc gcataagtcg atatatcgaa gccgccttgc tgacccaggc tgggttcggc
     2161 gatacctcca gggaactgtt gtatgtctac aaatgacaac ggcacccgca ggtgccgttg
     2221 atcgacgtag ctttgacctg acgagcggtt gcgccgccgg gtcatgcaac cttagcggtt
     2281 gcagacgtac atcgtgactt caaaaccgaa acgcatttca gtagcagctg gcttggtcca
     2341 catgatcgtt ctcctagttg gttggtcttg tactacgaac cgtgtcgctt gttcgtatgg
     2401 gtccactata cccagccctt ggatgtttcc ctatgtcagg gtcatgagtc gggcgtcgtg
     2461 ataatcatga atgacacctg catctaaacc ttagggtcag gaaggatcaa aagcccagct
     2521 tgagcttgat gcgacgaaag atctggcaca gtacgctgcc atcgagcttg tagtgcttat
     2581 gcaggggctg tttcacctcc cagctggctt tcaggccggc ggggaacgtg accaggtcgc
     2641 ccttgccgaa gg
//
LOCUS       DATMTW010000330         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_667238, whole genome shotgun sequence.
ACCESSION   DATMTW010000330 DATMTW010000000
VERSION     DATMTW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667238"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VN084_06495"
     CDS             complement(<1..1264)
                     /locus_tag="VN084_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWT29144.1"
                     /translation="MIQLKNLTLSRGVRVLVEGASFQLHPGHKVGLTGANGAGKSSLF
                     AMLRGELHAESGDLEIPPHWVIAHVTQETPALSEPAIEFVLDGDEELRVVERALQAAE
                     TAHRGDEVGELHARLGDIGGYSARSRAAALLHGLGFSDGDLVRPVSDFSGGWRVRLNL
                     ARALMCRSDLLLLDEPTNHLDLDAVIWLEGWLKDYRGTLLLISHDRDFLDAVVNHIAH
                     IEQQRLTLYRGGYSDFERQRAERLAQQQSLYERQQREVAHLHSYIDRFRAKATKARQA
                     QSRIKALERMELISAAHVDSPFSFAFRPTLAAPDPLLVLDEVDAGYRQQAVLRQIMLT
                     IRPGERIGLLGRNGAGKSTLVKVLAGAMAPIGGERIEGKDLAIGYFAQHQLEQLRPEE
                     SPLQHMLRLDQEHNQPQAREQDHRNYLGG"
     gene            1525..>4101
                     /locus_tag="VN084_06500"
     CDS             1525..>4101
                     /locus_tag="VN084_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HWT29145.1"
                     /translation="MVKKSLIWIYRATLTLLWLIIIVLACSVLLLRYVVLPKVDDYKD
                     QIAQRASEVAGQKITIGDIDASWDGLNPHFALFNVELYDAQQRPALTLKHVETSVSWL
                     SIPLLQPRLSAITIHKPELTIRREADGVLYIAGISMGGPARPEFPNWLLKQSRIDVVD
                     ATVLWQDDLRKAPPLTLNQLNLQILSPAWESLIGHHRFGLRATPSAGSSYPIDIRGNV
                     YGRDVSQPENWRGTLYGKLEGTDIAAWRNWISYPIDLREGFGAARFWLDFDQGQPQRV
                     TSDVLLSNVVTRISRSSPEATLNNLSGRLAWIRHSDGQEVRAEGIKIATAGGLNMQNG
                     NASVRERKVDGKDQVEGSIKLDEIRLQSLRTLATYLPIPKQTLDDINEIAPAGLVHAL
                     EASWKGDRESPDQYTVRGQFSELGMQAYQHFPGFTNLRGTLDADEKNGTLTIDAQRAE
                     IDMPKVLRWPIPADKLSGQVRWRKNAGKTDIRFSNVAVANAHLTGTINGSYQLNGIKG
                     GYLDLNARFGQADGKYAHYYYPRILGESTLHWLDSSILAGRGEDVNVVVRGNLDEFPY
                     IGNKNGLFRVSAKIKDGVLDYAAGWPRIDDLSLDLLFEGARMELNATGGRLLGSQIVK
                     GKVSIAELHAAQPVLDVNADGQGPVAEGIKYINNSPLVETLGGFSRNLRTAGNGKLHL
                     ELHIPLHDTHNTKIKGSYTVANAAMASDSIPELTRLTGKVDFTETGVRAQNVTAVAYG
                     GPVAFNVSTGSDGTTQIAARGRVTDTGLQQALGPGLADRLDGMTDWYADIAVHKTGTD
                     LLLRSNLLGMASTLPPPLAKAATDRMVLRVEKKQTAADQDLISLHLDNLLNAKLARVD
                     HGG"
BASE COUNT          867 a         1342 c         1184 g          708 t
ORIGIN      
        1 atcccccgag gtaattgcgg tgatcctgtt cgcgcgcctg aggctggttg tgttcctggt
       61 cgagtcgcag catatgctgc agcggcgact cttctggccg caattgttcc agctggtgct
      121 gagcgaaata gccgatggcg aggtccttgc cctcgatccg ctcgccgccg atcggtgcca
      181 tcgcgccagc cagtaccttg accagcgtgg atttgcccgc gccattgcgg cccagcaagc
      241 cgatgcgctc tcccgggcgt atggtcagca tgatctgacg caacaccgcc tgctgccggt
      301 aaccggcatc gacctcgtcc aataccagca gtgggtccgg ggctgccaag gtcggccgga
      361 aagcgaaact gaatggcgaa tcgacatggg cggcagagat cagctccatc cgttccagcg
      421 ccttgatgcg gctctgcgcc tgacgcgcct tggtcgcctt ggcacggaaa cggtcgatat
      481 aactgtgcaa gtgcgcgact tcgcgctgct gacgctcata caacgattgc tgctgcgcca
      541 gccgttcggc acgctggcgc tcgaaatcgg agtagccgcc gcggtacagg gtcaggcgct
      601 gctgttcgat gtgcgcgatg tggttgacca ccgcatcgag aaaatcgcga tcatgcgata
      661 tcaacagcaa ggtgccgcga taatccttga gccagccttc gagccagatg acggcatcca
      721 ggtcgaggtg attggtaggc tcgtccagca acagcagatc ggatcggcac atcagcgccc
      781 gcgcgagatt gagccgcaca cgccagccgc cggagaaatc cgagaccggt cgtacaaggt
      841 cgccgtcgct gaaaccaagc ccatgcaaca aggccgccgc gcgcgaccgg gccgaatagc
      901 cgccgatatc gcccaggcgc gcgtgcaatt cgcccacctc atcgccgcga tgagcggttt
      961 ctgcagcttg caacgcgcgc tccacgaccc gcaactcctc atcaccgtcg agcacgaatt
     1021 caatcgcagg ctcgctgagc gccggggtct cctgcgtgac atgggcgatc acccagtgcg
     1081 gcgggatctc aagatcgccc gactcggcat ggagttcacc gcgcaacatg gcgaacaggc
     1141 tcgacttgcc ggcgccgttg gcgcccgtga ggccgacctt gtgccccgga tgcaattgga
     1201 acgaggcgcc ttcgaccagg acgcgcacac cgcgggacaa cgtgagattc ttaagctgga
     1261 tcaagggagg acgccggaaa ggttttgtaa aaatacgtaa aagggcatca ttttaatgca
     1321 ttccatgcgg cccactcgca actttaggct gcaactttgt ctcacaggtg tcctgcaacc
     1381 gggattaact ggcaataatg aagtaaaatc aggtcatcta ttgatcgaag ccgcagatcg
     1441 agctttaaaa cgatgcccag ggcgaagcca ggggccagag catcgagccg gctttcaata
     1501 caacacctac ccgcaacaaa ggttatggtc aaaaaatcac tgatctggat ctaccgtgcc
     1561 acgctcaccc tgctctggct catcatcatc gtccttgcat gctcagtcct cttgttgcgt
     1621 tatgtcgtgt tgcccaaggt cgatgattac aaggaccaga tcgcccagcg cgcgagcgaa
     1681 gtcgccggcc agaagatcac catcggcgat atcgacgcca gctgggatgg cctcaatccg
     1741 cacttcgcgc tattcaacgt cgagctttac gatgcccagc agcgcccggc gctgacgctc
     1801 aagcatgtcg agaccagcgt ctcctggctc agcatcccct tgctgcagcc gcgtctgtcg
     1861 gcaatcacca tccacaagcc ggaactcacc atccgccgcg aagccgacgg cgtgctctat
     1921 atcgctggca tctcgatggg cgggccggca cggccggagt tccccaactg gctacttaag
     1981 caatcacgca tcgatgtcgt cgatgccacc gtgctctggc aagacgatct gcgcaaggca
     2041 ccgccgctga cgctgaacca gctcaatctg cagatcctca gcccagcctg ggaatcgctc
     2101 atcggccatc accgcttcgg cttgcgcgcc acgccatcgg ccggctcttc ctatcccatc
     2161 gatatccgcg gcaatgtcta tggccgcgac gtgagccaac ccgagaactg gcgcggcacg
     2221 ctatatggca aactcgaagg caccgatatc gccgcctggc gcaactggat cagctatccg
     2281 atcgatctgc gcgagggatt cggagcggcc aggttctggc tggatttcga ccagggccaa
     2341 ccgcaacgcg tgaccagtga cgtgctgttg agcaacgtcg tcacgcgcat ctcccgcagt
     2401 agccccgaag ccacactcaa taacctttcc ggccggctcg cctggatacg gcacagcgat
     2461 ggccaggaag tccgcgcgga gggcatcaag atcgccaccg ccggcggcct gaacatgcag
     2521 aacggcaacg ccagcgtgcg agaacgcaag gtcgacggca aggatcaagt cgagggcagc
     2581 atcaagctcg acgagatccg cctgcaatca ctgcgaacgc tcgcgaccta cctgcccatc
     2641 cccaaacaga cactggacga tatcaacgag atcgcgcccg ctggtctggt ccatgcgctc
     2701 gaggcaagct ggaaaggcga ccgtgaatcg cctgaccaat atacggtgcg cggccagttc
     2761 agcgagcttg gcatgcaggc ctaccagcat tttccgggat tcacgaatct gcgcggtacg
     2821 ctcgacgccg atgagaagaa cggcacgctg accatcgacg cccagcgtgc cgagatcgat
     2881 atgcccaaag tgctgcgctg gccgatccct gccgacaagc tcagcggcca ggtcaggtgg
     2941 cgcaagaacg ccggcaagac cgatatccga ttcagtaacg tggctgtcgc caacgcgcac
     3001 cttaccggca ccatcaacgg cagctaccag ctcaacggca tcaagggcgg ctatctcgac
     3061 ctcaatgcgc gtttcggcca ggccgacggc aaatatgccc attactacta tccgcggatc
     3121 ctcggcgaaa gcaccctgca ctggctggac agctcgatcc tggccggccg cggtgaagac
     3181 gtgaatgtgg tggtgcgtgg caatctcgat gaatttccct acatcggcaa caagaacggc
     3241 ctgttcaggg tcagcgccaa gatcaaggat ggcgtcctgg actacgctgc cggctggccg
     3301 cgcatcgacg acctcagcct cgatctgctg ttcgaaggcg cacgcatgga attgaatgcc
     3361 actggcggtc gcctgctcgg tagccagatc gtcaagggca aggtgtcgat tgccgagctg
     3421 cacgccgcgc aacccgtgct cgacgtcaac gccgatggcc agggcccggt cgccgagggc
     3481 atcaagtaca tcaacaacag cccgctcgta gagacccttg ggggcttcag ccgtaacctt
     3541 cgcacagccg gcaacggcaa gctgcacctg gagctgcata tcccgctgca tgacacccac
     3601 aacaccaaga tcaagggcag ctacaccgtg gccaacgcgg cgatggcaag cgacagcatc
     3661 cccgaactca cgcgcctgac aggcaaggtc gatttcaccg aaaccggcgt acgcgcgcag
     3721 aacgtcaccg ctgtcgctta tggcggtccg gtcgccttca acgtcagcac cggcagcgat
     3781 ggtacgaccc agatcgccgc ccgtggccgc gtcaccgata ctggcctgca acaggcactc
     3841 gggccggggc tggccgaccg cctcgacggc atgaccgact ggtatgccga tatcgccgtg
     3901 cacaagaccg gcaccgacct cttgttgcgc tccaacctgc tcggcatggc ttccacgcta
     3961 ccgccgccgc tggccaaggc tgcgaccgat cgcatggtgc tacgtgtcga gaaaaagcag
     4021 acggctgcgg atcaagacct catcagcctg catctcgaca acctgctcaa cgcaaaactg
     4081 gcgcgcgtgg atcatggtgg c
//
LOCUS       DATMTW010000331         1994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_667340, whole genome shotgun sequence.
ACCESSION   DATMTW010000331 DATMTW010000000
VERSION     DATMTW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667340"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VN084_06505"
     CDS             <1..263
                     /locus_tag="VN084_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWT29146.1"
                     /translation="HQLTRAFGVHIDEEGLALRGTFVINPEGVIKTQEIHDNAIARDV
                     KETLRKLKAAQYVANNPGQVCPAKWNEGAATLTPSLDLVGKI"
     gene            340..1896
                     /gene="ahpF"
                     /locus_tag="VN084_06510"
     CDS             340..1896
                     /gene="ahpF"
                     /locus_tag="VN084_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HWT29147.1"
                     /translation="MLDANIKNQLQAYLEKVTLPIEIVANLDDSDKSREMLEMLQDIA
                     GLSAKITLEQRHDKDERAPSFSLNRPGENIGLRFAGIPMGHEFTSLVLALLQVGGHPP
                     KVEPEVIEQVKHLEGTFHFETFVSLTCHNCPEVVQALNLMAVLNPNITHVMIDGGLYQ
                     QEVEARQIMAVPTIYLNGQDFGAGRMELGEILAKIDTGAAKRDAEKLSTKAPYDVLII
                     GGGPAGASAAVYAARKGIRTGIVADRFGGQVMDTLGIENFISVKATEGPKLVMALEEH
                     VKSYEVDIMNLQRAQALAKHGNSDYLELKLENGATLKSKSVIIATGARWREMNVPGEK
                     EYRGKGVAYCPHCDGPLFKGKNVAVIGGGNSGVEAAIDLAGIVGHVTLIEYDTKLKAD
                     AVLQNKLFTLPNVTVVINAQTTEITGDGQKANGLLYKDRATGNEHRLAVEGIFVQIGL
                     LPNTDWLKGTVDLSKYGEIKVDAHGQTSLPGVFAAGDVTTVPYKQIIIAMGEGSKAAL
                     GAFDYLIRQD"
BASE COUNT          458 a          608 c          585 g          343 t
ORIGIN      
        1 cccaccagct gacccgcgct ttcggtgtac acatcgacga agaaggcctg gcactgcgtg
       61 gcactttcgt catcaatccg gaaggcgtga tcaagaccca ggaaatccac gacaacgcca
      121 tcgcgcgtga cgtcaaggag accctgcgca agttgaaggc cgcgcaatac gtggctaaca
      181 atccaggcca ggtctgcccg gccaagtgga acgaaggcgc agcgaccctg accccgtccc
      241 tggatctggt cggcaagatc taagtaacaa gcatggagca atagacgccc gggcatctcg
      301 cccgggcagt actacccaga cattttaagg aacctcatca tattggacgc caatatcaag
      361 aatcagctgc aggcttatct ggaaaaagtg accctgccga tcgagatcgt cgccaatctg
      421 gacgacagcg ataaatcgcg cgaaatgctg gagatgctcc aggatatcgc cgggctgtct
      481 gcgaagatca cgctcgaaca gcgtcatgac aaggatgagc gtgccccttc gttctcattg
      541 aaccgccccg gggaaaacat cggcctgcgt ttcgccggca tccccatggg ccacgaattc
      601 acatcactgg tgctggcact gctgcaggtc ggcggccacc caccgaaagt cgagcccgag
      661 gtcatcgagc aggtgaaaca tctcgaaggc accttccatt tcgagacctt cgtttcgctc
      721 acctgccata actgcccgga agtcgtgcag gcgctgaacc tgatggccgt gctcaacccg
      781 aatatcaccc acgtgatgat cgacggcggc ctgtaccagc aggaggtgga ggcgcgccag
      841 atcatggcgg tgccgaccat ctacctcaac ggccaggact tcggcgctgg ccgcatggag
      901 ctgggcgaga tcctcgccaa gatcgacacc ggtgccgcca agcgcgatgc cgagaagctc
      961 tcgaccaagg cgccttatga cgtattgatc atcggtggcg gcccggcagg cgcttctgcc
     1021 gcagtgtatg ccgcccgcaa gggtatccgt accggtatcg tcgccgaccg tttcggcggc
     1081 caggtaatgg atacgctcgg catcgagaac ttcatctcgg tgaaagcgac cgaaggcccc
     1141 aagctggtga tggcgctgga agagcacgtc aagagctacg aggtcgacat catgaacctg
     1201 cagcgtgcgc aggcgctggc caagcacggc aacagcgact atctcgaact caagctcgag
     1261 aacggcgcca cgctgaagag caaatcggtc atcatcgcca ccggcgcacg ctggcgcgag
     1321 atgaacgtgc ccggcgaaaa ggaataccgc ggcaagggag tcgcctactg cccgcactgt
     1381 gacggcccgc tgttcaaggg caagaatgtc gccgtgatcg gcggcggcaa ctccggtgtc
     1441 gaagcggcga tcgacctcgc cggcatcgtc ggccatgtca cgctcatcga gtacgatacc
     1501 aagctcaagg ccgatgccgt gttgcagaac aagctgttca cgttaccgaa cgtgacggtc
     1561 gtcatcaacg cgcagaccac cgagatcacg ggcgacggtc aaaaggcgaa cggcctcctc
     1621 tacaaggatc gcgcaaccgg taacgaacat cgcctggcgg tcgaagggat cttcgtacag
     1681 atcggcttat tgccgaacac cgactggctg aaaggtaccg tcgatctcag caagtatggc
     1741 gagatcaagg tcgatgccca tgggcagact tcgttgccgg gcgtattcgc cgcgggtgac
     1801 gtgacgaccg tgccttacaa gcagatcatc atcgccatgg gcgaggggtc caaggccgcg
     1861 ctcggcgcct tcgattacct gatccggcag gattgagcgg cagcatactg atctgtgagt
     1921 cgtaccatga aaaagccagc gcaagctggc tttttctttg tcctccggat tgatgcaagg
     1981 caaaaacccg tcac
//
LOCUS       DATMTW010000332         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_498786, whole genome shotgun sequence.
ACCESSION   DATMTW010000332 DATMTW010000000
VERSION     DATMTW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498786"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1849
                     /locus_tag="VN084_06515"
     CDS             <1..>1849
                     /locus_tag="VN084_06515"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWT29148.1"
                     /translation="TRKQFLYSLSEDARAAGPTLIPKRQAIQSRIYAALHEHGTLDEA
                     ALAACSPGWRKAVSAMQMQGWLEAREVIAGLTPSTASPALAPALNADQQAACDAIAQS
                     LHGFKPWLLYGITGSGKTEVYMRLIEQVLMDATAQVLVLVPEINLTPQLEARFRSRLP
                     GFPLVSLHSNLGESERLQNWRLAQSGQARIVIGTRLSVLTPMPALKLIIVDEEHDGSY
                     KQQDSMRYHARDVALVRAKLLDIPIVLGSATPALETWHNAMQGKYCLLRLNERAVAQA
                     VLPQIRCIDTSRMPLQDGLSPALLQALRDRVAKGEQSLLFINRRGFAPVLLCNACQWI
                     APCSRCSSRLVVHLRQGRLRCHHCGHEERIARQCPSCGNADLHPTGQGTQRLEQTLAQ
                     LLPEARILRVDRDSTRRKDALSEMLSAVHADEVDILVGTQMLAKGHDFPNLTLVGVID
                     TDSALFSPDFRAAERLFAQLMQVAGRAGRADKAGEVLIQTAFPDHALFNALRTQDYSA
                     YAATLLHERELTLFPPYQHLALIKAEANDFGQVSRFLGDAADCARALANDDVLVYDAV
                     RPQMERLKGMERGQLLMQADNRPALQRLLRTLVPQLRAHVLSPKVRWAVD"
BASE COUNT          344 a          578 c          585 g          342 t
ORIGIN      
        1 cacgcgcaaa cagttcctct atagtctatc ggaggacgcc agggcggctg gcccgaccct
       61 aatcccgaag cggcaggcga tccagtcacg catctacgcg gcgctgcatg aacacggcac
      121 gctggacgag gcagcattgg cagcctgctc gccgggctgg cgcaaggccg tatcggcgat
      181 gcagatgcaa ggctggcttg aggcgcgcga ggtcattgcc gggctgacgc catccaccgc
      241 atcgccggcg ctggcccctg ccctcaacgc cgaccagcag gcagcgtgtg acgctatcgc
      301 tcagtcgctg catggcttca aaccctggct gttgtacggc atcacgggga gcggcaagac
      361 cgaagtctac atgcggctga tcgaacaggt gctcatggat gcgacagcgc aggtactggt
      421 gctggtgccc gagatcaatc tcacgccaca actggaagcg cgctttcgca gccgcctgcc
      481 cggttttccg ctggtcagtc tgcacagcaa tctaggcgag agtgagcgct tgcaaaactg
      541 gcgcctggca caatctggcc aggcccgcat cgtcatcggc acgcggctgt cggtgctcac
      601 gccgatgccg gcgctcaagc tcatcatcgt tgacgaagag catgacggct cctacaagca
      661 gcaggacagc atgcgctacc atgcgcgcga tgtcgcactg gtgcgcgcca agctgctgga
      721 tatccccatc gtgttgggtt cggccacacc ggctttggag acatggcata acgccatgca
      781 gggcaaatat tgcctcttgc gactgaacga gcgtgcggtg gcgcaggccg tgttgccgca
      841 gatccgctgc atcgatacat cgcgcatgcc gcttcaggac ggattatcgc cagcgttgct
      901 gcaagccttg cgtgatcgtg tggccaaagg cgagcaaagc ctgttattca tcaaccgcag
      961 gggtttcgcg ccggtactgt tgtgcaatgc ctgccagtgg atcgcgcctt gctcgcgctg
     1021 cagcagccgg ctggtcgtgc atctgcgcca agggcgactg cgctgccatc attgcgggca
     1081 tgaagaacgc atcgcgcgcc aatgtccaag ctgtggcaat gcggatctgc acccgaccgg
     1141 ccagggtacg caacggctgg agcagacgct agcccagctc ttgccggaag cccgtatctt
     1201 gcgcgttgac cgcgacagca cgcggcgcaa ggacgccttg agcgagatgc tctctgcggt
     1261 gcatgcggat gaagtggata tcctcgtcgg cacgcagatg ctggcaaagg gccacgattt
     1321 ccccaacctc acgctggtcg gcgtcatcga taccgacagt gcgctgttca gcccggattt
     1381 tcgtgcggca gagcgtctgt tcgcgcaact catgcaagtc gcggggcgcg ccggccgagc
     1441 cgataaggca ggcgaggtgc tgatccagac ggccttcccc gaccatgcgt tgttcaatgc
     1501 cctgcgtacg caggattaca gcgcctacgc agcgacgtta ttgcatgagc gcgaactcac
     1561 gctgtttccg ccctatcagc atctggcgct catcaaggcc gaagccaacg atttcgggca
     1621 ggtctcgcgc tttctcggtg atgcggccga ttgtgcacgc gctctggcga atgacgatgt
     1681 gctggtctac gatgcggtca ggccgcagat ggaacggctg aaaggcatgg agcgtgggca
     1741 gttgctgatg caggccgaca atcgcccggc cttgcaacgc ctgctacgca cgctggttcc
     1801 gcaactacgt gcgcatgtcc tgagccccaa ggtgcgctgg gcggtcgat
//
LOCUS       DATMTW010000333         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_296836, whole genome shotgun sequence.
ACCESSION   DATMTW010000333 DATMTW010000000
VERSION     DATMTW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296836"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VN084_06520"
     CDS             <1..538
                     /locus_tag="VN084_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29149.1"
                     /translation="CGVQLPEMFAQQLYFMARGALQEALDADAAETIAKVKEAASALI
                     RAQSEKEPRITKPAYAIAASLLVLSGICGAMVMHLWHDSITPAQAENSSMLRTQNALH
                     NPANTAEIYAVMERMRAGVCQFPEALLLPEAQKKVYVELIVDGLIPITPEDQAVARAL
                     IQKVRCNYTPMLMSNSVG"
     gene            732..1301
                     /locus_tag="VN084_06525"
     CDS             732..1301
                     /locus_tag="VN084_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWT29150.1"
                     /translation="MSLLREKILNVATDLFQTRGINSTGVDTIVAVAGTTKMTLYKYF
                     RSKEDLILEVLQKGHQDFQAWLSDKLSSNARKPSEKLQKLFDFIEEWVDSPDFHGMAF
                     LKASAEFPSEDNAVHQLSAEHSRQFRLYLTQLATDAGVKDADSLSLQLSLLIEGAIQA
                     EQMRRGSGAVKYAKKAAKILIDSALKDSR"
     gene            complement(1306..2952)
                     /locus_tag="VN084_06530"
     CDS             complement(1306..2952)
                     /locus_tag="VN084_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWT29151.1"
                     /translation="MNNLAELLLQWAAEPQKVLYRQYQEGAWQDYTVATLLDLAARWQ
                     EAYRGAGLRSGDRVAICMKNGVQWVLADLAALASGLVVVPLYVDDNPDNLGWCLQDAG
                     AALVVADHLRFLPRLQTLGITLPTIVLVRGEPVTSVVTLADWLPETAPPLSVADQPAE
                     ALATIVYTSGTVGRPKGVMLSHRNILSNITSILGAYDVYVSDRLLSVLPLTHMFERTA
                     GYYLALQAGAEVTYCRGINELAQDLATQQPTVIMAVPRLFERILRRLDDKLRAAPLRR
                     WLFKLAVGAGWRRFSAQARFYDALLLKTVHPMIARPLLAGLGGRLRHAVVGGAALDQR
                     VARTFIGLGVPLLQGYGLTEASPVVAVNRQDHHDPASVGEPLPGLETRVNSAGELLVR
                     GASVMLGYWNSPEATQACIDAEGWLNTGDLVDIKARRIYIRGRSKHILVLSNGEKIAP
                     EDIESAILRDPLFEQVLVVGEGRPFLVLLVVSSSGDAADLLRRANLCVRHLPRYARIR
                     RVVLADAAWEVENGLMTPTLKIKRNAVIARYQPQIEAVYR"
     gene            3025..3984
                     /locus_tag="VN084_06535"
     CDS             3025..3984
                     /locus_tag="VN084_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWT29152.1"
                     /translation="MHTLICGSLAFDTIMVFGDRFKHHILPEKIHILNVAFLVPEMRR
                     EFGGTAGNIAYNLQLLEGNPLIMGTVGEDFASYRAWLERHQLDTRHIKTIPGSFTAQA
                     FITTDLDDNQITAFHPGAMNESHQNSVKDTQDVSLAIIAPDGRDGMFQHARECHEAGI
                     PFMFDPGQGLPMFNGEELMHFIELADYMAVNDYEAQLLQEKTGHSVETLAEKLKALIV
                     TLGGQGSIIYADGQRFDIPCVEALEVVDPTGCGDAYRAGLLYGIARGWDWPTCGRLAS
                     TMGAIKIASRGPQNHKPSREEIQAIYSQALVNEVLSEEKRLNG"
     gene            3995..>4225
                     /locus_tag="VN084_06540"
     CDS             3995..>4225
                     /locus_tag="VN084_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29153.1"
                     /translation="MRHNTMIVITAVALLLGGCASSNSGSVYSRDEARRVQTVKTGVV
                     ESVRQVKLEGTKSPVGTAGGAVVGGVAGSQVGH"
BASE COUNT          953 a         1287 c         1198 g          787 t
ORIGIN      
        1 ctgtggcgtg caactgccgg agatgttcgc ccagcagcta tatttcatgg cccgcggcgc
       61 attgcaggaa gcgctcgatg ccgatgccgc cgagaccatc gccaaagtga aggaagccgc
      121 cagcgccctc atccgggccc aatccgagaa agaaccgagg atcaccaagc ctgcgtatgc
      181 catcgcagcc agcctactcg tactgagcgg gatctgcggc gccatggtga tgcatctatg
      241 gcatgacagc atcactccgg cgcaagcaga gaacagctcg atgctgcgca cccagaatgc
      301 gttgcacaat cctgccaata ctgccgaaat ctacgctgtg atggagcgga tgcgcgcagg
      361 cgtatgtcag ttccctgagg ctttgctgct gcctgaggca caaaagaagg tatatgtaga
      421 gctgatcgtc gacggcctca tccccatcac ccccgaagac caggccgttg cccgggctct
      481 gatccagaaa gtacgctgca attacacacc gatgctgatg tcgaactcgg ttggctgagt
      541 cacacaattg cacacgagcc ggcgatgccg gcttttttat cgcccttcac cttgcttatt
      601 acagaaccgc acagacgctg gcagagcaat tcgattcaac tgatgcatcg aatatcgatg
      661 caaggtatac tgtacggtat atatttttcc caatgaatac gttagactgc gaacaaacag
      721 gaagatagac catgtccttg ctcagggaaa agattttgaa tgtcgccacc gatctgtttc
      781 agactcgggg catcaactcg actggtgtcg acaccatcgt cgctgtcgcc ggcacgacca
      841 agatgacgct gtacaagtat ttccgcagca aggaagacct catccttgaa gtgttgcaga
      901 aagggcatca ggactttcag gcctggctga gcgacaaact cagcagcaat gcacgcaaac
      961 cctcagaaaa actgcaaaag ctgttcgatt tcatcgagga atgggtcgac tcacccgatt
     1021 tccatggcat ggctttcctc aaggcctctg cggaattccc cagcgaagac aatgcagtcc
     1081 atcagttatc ggctgaacat tcacgccagt tccggctata tctgacgcag ctcgcgaccg
     1141 atgcgggcgt caaggatgcg gatagcctct cgttgcaact ctcattgctg atcgaaggcg
     1201 cgatccaggc tgagcagatg cgccgcggtt ctggcgcagt gaagtatgcc aagaaagcgg
     1261 ccaagatcct gatcgacagc gcgctcaagg attcacgcta ggcggttatc ggtataccgc
     1321 ctcgatctgc ggctgataac gcgcgatcac ggcgttgcgt tttatcttca atgtcggcgt
     1381 catgagcccg ttctcgacct cccacgccgc atccgccaga acaacacgac ggatcctggc
     1441 ataacgcggc agatgccgta cgcacagatt ggcgcgtcgc aacaaatccg cagcatcccc
     1501 gctcgaactg acgaccagca acaccaggaa cggtcgtccc tcgccgacca ccaatacctg
     1561 ttcgaacaac gggtcgcgca gtatcgccga ttcgatatcc tctggcgcga tcttctcccc
     1621 attggacaag accagtatgt gcttactgcg acctcggata tagatgcggc gcgccttgat
     1681 atcgacaaga tcccctgtgt tgagccagcc ttcggcgtcg atgcaggcct gtgtcgcctc
     1741 tgggctgttc cagtagccca gcatcaccga cgcgcctcgc accagcaact cacctgctga
     1801 attcacccgc gtttcgaggc caggcaaggg ttcgccgaca gatgcagggt catgatggtc
     1861 ctggcgattg acagccacca ccggcgacgc ttccgtcaag ccatagccct gcagcaaggg
     1921 cacacccaag ccgatgaaag tccgtgcgac ccgctggtcg agtgccgcac cacccacgac
     1981 cgcatgacgc agccgcccgc ccagacccgc caacaggggt ctggcaatca ttggatggac
     2041 ggtcttcagt agcaaggcat cgtagaagcg cgcctgtgcg ctgaaacgtc gccagccagc
     2101 gccgacggcg agcttgaaca gccaacgccg caagggcgct gcgcgaagct tgtcatcgag
     2161 acgccgcagg atacgctcaa acagtcgggg aaccgccatg atcacggtcg gctgttgcgt
     2221 cgccaggtcc tgggccagtt cattgatgcc gcgacagtac gtgacctccg cgccagcctg
     2281 cagcgccaga tagtagccgg cggtccgttc gaacatatgg gtcagcggca ataccgagag
     2341 caggcgatcg ctgacataga cgtcatacgc gccgaggata ctggtgatat tgctaaggat
     2401 attgcgatgc gaaagcatga cccctttagg ccgaccgact gtgcccgagg tgtagacgat
     2461 ggtcgcgagt gcttctgcag gctgatcggc cacgctcaac ggcggcgccg tttcgggcag
     2521 ccagtctgcc agcgtaacga ccgaggtgac gggctcgccg cgcaccagca cgattgtcgg
     2581 cagcgtgatg cccagggtct gcaggcgagg cagaaaacgc aagtggtcag caaccacgag
     2641 ggcggcgccg gcatcctgca ggcaccagcc aagattatcc gggttgtcat cgacgtaaag
     2701 cggaacgacc accaggcctg acgccagcgc ggcgagatcg gcgagcaccc attgcacgcc
     2761 gttcttcatg caaatggcga cccggtcacc agatcgcagg ccggctccgc gatatgcctc
     2821 ttgccagcgc gctgccagat cgagcaaggt ggcaaccgta tagtcttgcc aagccccctc
     2881 ctgatactgc cgatagagca ccttttgcgg ctctgcggcc cactgcagca gcaattcagc
     2941 cagattgttc atgactcagc gcccaatgct tgcccctgac gctgacgctc gggataatgc
     3001 aggttttatt cgaaaagaat gtacatgcat actttgatct gcgggtcgtt agcgttcgat
     3061 accatcatgg tattcgggga ccgtttcaag catcacatcc tgcctgaaaa aatccacatc
     3121 ctcaatgtcg cgttcctggt cccggagatg cgccgcgagt tcggaggcac ggcaggcaat
     3181 atcgcgtaca acctgcagct gctggaaggc aatccgctga tcatgggcac cgtgggcgag
     3241 gatttcgcca gctaccgcgc ctggctggag cgccaccagc tggatacccg gcacatcaag
     3301 accattcccg gcagtttcac tgcccaggcc ttcatcacca ccgacctcga cgataaccag
     3361 atcaccgcct tccacccggg cgcgatgaac gaatcgcacc agaacagcgt aaaggacacc
     3421 caggacgtga gcctcgccat catcgcgccg gacggccgcg acggcatgtt ccagcatgca
     3481 cgcgaatgcc acgaggccgg catcccgttc atgttcgatc ctggtcaggg gttgcccatg
     3541 ttcaacggtg aggagctcat gcacttcatc gagcttgcgg attacatggc cgtcaacgac
     3601 tatgaggcac aactattgca agagaaaaca ggtcatagtg tagagacgct ggcagagaag
     3661 ctgaaagcgt tgatcgtcac gctgggtgga caaggttcga tcatctatgc cgacggtcaa
     3721 cgttttgaca tcccttgcgt tgaagctctg gaagtcgtgg atccgacagg ttgcggtgac
     3781 gcctatcgcg caggtttgct ctacggcatc gcccgtggct gggattggcc gacctgtggc
     3841 aggctggcat cgaccatggg ggcgatcaag atcgccagcc gcggcccgca gaaccacaag
     3901 ccgtcgcggg aagagatcca ggcgatctac agccaggccc tggtcaatga agtattgtcc
     3961 gaagagaaaa gactgaatgg ataaggagtc catgatgcgc cataacacca tgatcgtgat
     4021 caccgctgtc gccctactgc tcggcggctg cgccagttcg aattcgggca gcgtctacag
     4081 tcgcgatgaa gcgcgacgcg tacagaccgt caagaccggg gtcgtcgaaa gcgtacgcca
     4141 ggtcaagctc gaaggcacca aatcgccggt aggtacagca ggcggcgcgg tcgtcggcgg
     4201 tgtcgccggc agccaggtcg gccac
//
LOCUS       DATMTW010000334         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_600486, whole genome shotgun sequence.
ACCESSION   DATMTW010000334 DATMTW010000000
VERSION     DATMTW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600486"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VN084_06545"
     CDS             complement(<1..1631)
                     /locus_tag="VN084_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWT29154.1"
                     /translation="MALLSIAAFAPGAWAADALTGAVQKQPLNVSAILMFVAFVGATL
                     CITYWASKKNKSASDYYAAGGKITGFQNGLAIAGDYMSAASFLGISALVFTSGYDGLI
                     YSIGFLVGWPIILFLIAERLRNLGKYTFADVASYRLGQTQIRSLSACGSLVVVAFYLI
                     AQMVGAGKLIQLLFGLDYHVAVILVGILMCMYVLFGGMLATTWVQIIKAVLLLSGASF
                     MALMVMKHVNFDFNMLFSEAIKVHPKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLP
                     HILMRFFTVSDAKEARKSVLYATGFIGYFYILTFIIGFGAILLVSTNPAFKDAAGALL
                     GGNNMAAVHLANAVGGSIFLGFISAVAFATILAVVAGLTLAGASAVSHDLYASVIKKG
                     KANEKDEIRVSKITTIALAVLAIGLGILFESQNIAFMVGLAFSIAASCNFPVLLLSMY
                     WKKLTTRGAMIGGWMGLVSAVGLMVLGPTIWVQILHHEKAIFPYEYPALFSMIIAFIG
                     IWFFSITDKSTAADNERALFFPQFVRSQTGLGASGA"
     gene            complement(1643..1954)
                     /locus_tag="VN084_06550"
     CDS             complement(1643..1954)
                     /locus_tag="VN084_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HWT29155.1"
                     /translation="MNDSIYLSIQNSPRFKELVSKRERFAWILSAIMLGLYSGFILLI
                     AYGPQVLGAKISPESSITWGIPIGVGLIVSAFILTGIYVRRANGEFDDLNNAILKEAQ
                     Q"
BASE COUNT          493 a          628 c          607 g          385 t
ORIGIN      
        1 gccccactcg cccccaaacc agtctgcgaa cgaacgaact gcgggaagaa cagcgcccgc
       61 tcgttgtcag ccgccgtcga cttgtcggta atcgagaaga accagatccc gataaaggca
      121 atgatcatcg agaacagtgc agggtactca tacgggaaga tcgccttctc gtgatgcagg
      181 atctgcaccc agatggtcgg gccaagcacc atcaagccca cagcgctcac caggcccatc
      241 cagccaccaa tcatcgcgcc gcgagtggtc agctttttcc agtacatgga aagcagcagc
      301 accgggaagt tgcagctcgc cgcgatagag aatgccaggc cgaccatgaa cgcgatgttc
      361 tggctttcga aaaggatgcc gaggccgatc gccagcaccg ccagggcgat ggtggtgatt
      421 ttcgacacgc ggatctcgtc cttctcgttg gccttgccct tcttgatcac gctggcgtac
      481 agatcatggg acaccgccga ggcaccggcg agggtcaggc cggccaccac tgccaggatg
      541 gttgcgaacg ctaccgccga gatgaagccc aggaagatac tgccaccgac cgcattggcc
      601 aggtgcaccg ccgccatgtt gttaccgccc aacagcgcgc cagccgcatc cttgaaggcc
      661 gggttggtgc tgaccagcag gatcgcgccg aagccgatga tgaaggtcag gatatagaag
      721 tagccgatga agccggtggc gtacaacacg ctcttgcgcg cttcttttgc gtcactcacg
      781 gtgaagaagc gcatcaggat gtgcggcagg ccagcggtac cgaacatcag cgccaacccc
      841 agggagaatg ccgagatcgg atctttcacc aggccgcccg ggctcatgat cgcttcacct
      901 ttagggtgaa ccttgatcgc ctcggagaac aacatgttga agtcgaagtt gacgtgcttc
      961 atcaccatca gcgccatgaa ggaagcgccg gacagcagca acacagcctt gatgatctgc
     1021 acccaggtgg tcgccagcat gccgccgaac agcacgtaca tgcacatcag gataccgacc
     1081 aggatcaccg caacgtggta gtcgagaccg aacagcagct ggatcagctt gccggcaccg
     1141 accatttgcg cgatcaggta gaacgccacc acgaccagtg aaccgcaagc ggacagcgag
     1201 cggatctggg tttgccccag gcgataggac gccacgtcgg caaaggtgta tttgcccagg
     1261 ttgcgcaggc gctcggcgat caggaacaga atgatcggcc agcccaccag gaagccgatc
     1321 gagtagatca ggccatcgta gccggaggta aacaccagcg cggaaatacc caggaaggac
     1381 gccgccgaca tgtagtcacc agcaatcgcc aggccgttct ggaagccggt gatcttgccg
     1441 cccgccgcgt agtaatcgga ggccgacttg tttttcttgg acgcccagta ggtgatgcac
     1501 agggtcgcgc caacgaatgc gacgaacatc agaatcgccg acacgttaag cggttgtttc
     1561 tgcacggcgc cggtcagggc gtcagccgcc caggcgcccg gtgcgaaggc tgcgatgctc
     1621 aagagagcca ttagacgccg gatcattgct gagcctcctt gagaatcgca ttgttcaagt
     1681 cgtcgaattc gccattggcg cgtcgtacgt agatcccggt caggatgaag gctgaaacaa
     1741 tcagcccgac acctatcggt atgccccagg taatcgatga ctcagggctg attttcgccc
     1801 ccagcacctg cggcccgtaa gcaatcaaaa ggatgaatcc ggagtaaagc cctagcatga
     1861 tcgccgaaag aatccaggcg aatcgttccc tcttgctaac cagctccttg aatcgcgggc
     1921 tgttttgaat cgagaggtaa atgctgtcgt tcattgtttt tatcctcgca gcacagattt
     1981 agttggaacg gtatccactc tatgcggctg cggggcaggt tccagacgac cttagtctta
     2041 gaaccacatg acgttttcgc caattgcagg agcgagcttg ctcgcgatga atttgaaggc
     2101 gccgcacttt ttc
//
LOCUS       DATMTW010000335         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_667670, whole genome shotgun sequence.
ACCESSION   DATMTW010000335 DATMTW010000000
VERSION     DATMTW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667670"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..498
                     /gene="greB"
                     /locus_tag="VN084_06555"
     CDS             4..498
                     /gene="greB"
                     /locus_tag="VN084_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HWT29156.1"
                     /translation="MADQKNYITPEGFATLEAEFHQLLKVERPEVVRTVAWAASNGDR
                     SENGDYIYGKKRLRQIDSRLRFLMKRMDNAEVVDPKTQQGLEKVYFGAWVTLYSLDRD
                     TEHTYRIVGQDELEPSKGYISWVSPLARSMLGKQVGDVVRVQTPGGEEAYEITAIDYL
                     AAEH"
     gene            604..975
                     /locus_tag="VN084_06560"
     CDS             604..975
                     /locus_tag="VN084_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29157.1"
                     /translation="MRTQMQLDQLQGAAETHMLASHPCHDMPGMQVSGHDDHATHGAM
                     AMHDMEKPGATDAGKQDRNGHCQGHDCFACGICLHATNFTLTVMPPMPAPMLSHAKPV
                     THETVFASLRYPPALKPPIDA"
     gene            1013..1669
                     /locus_tag="VN084_06565"
     CDS             1013..1669
                     /locus_tag="VN084_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29158.1"
                     /translation="MNRNNLATLALLALALPLPGHAAALMGFADSTMFMGEWSADYQE
                     IMANYSPSFGHAIGIESMQMRDKDGMRSGIAAINYTGIFKRWNLPQGQANLWFTGGIG
                     ESYGSDRQGFAYTPGLQFDYETTRVYFLARARMMRAPGMNFDTAAVQGGFSFYETSYE
                     HTQPWFVVEARTMRNMDPAMQVTPALRLINKDYFLELGLTNPWDEGVRMPRINFMLTF
                     "
     gene            1710..2030
                     /locus_tag="VN084_06570"
     CDS             1710..2030
                     /locus_tag="VN084_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HWT29159.1"
                     /translation="MLGMTIAFSTLMFSSSAFATSSIKAKVNGMVCAFCAKGIEKKLK
                     ALPQTQSVFVDLKARVVALELKDGQTVPIEDFRKIIRDAGYEVAEAEVVDRSADQIKA
                     EVTQ"
     gene            2027..2431
                     /locus_tag="VN084_06575"
     CDS             2027..2431
                     /locus_tag="VN084_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29160.1"
                     /translation="MSTIEPLATDTALAASRRLNVFSLFTSFSTLICCALPALLVALG
                     AGAALSTLVSNVPQLVWISDHKPEVFGAAGLMLLVAGYFQWRARHAPCPADPALAAVC
                     RRARRNARWTYWISVGIYAVGAWFAFVAPLLL"
     gene            2517..3701
                     /gene="earP"
                     /locus_tag="VN084_06580"
     CDS             2517..3701
                     /gene="earP"
                     /locus_tag="VN084_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HWT29161.1"
                     /translation="MTAPDKPQRWDIFCRIVDNYGDIGVCWRLARQLTDDHGIRVRLW
                     VDDLAVAQRLIPAIDLQLPSQRIEDVEIAHWVADFPDTTVADVVIEAFACELPPRYLN
                     AMASARPVWLNLEYLSAESWVADFHLRPSPHPRLPLTKYFYFPGFVEESGGLLRERGL
                     ITAREAFQRSPQAQAAFCRRLGIDRLRSATSGDRDSANSDSMTMRVSLFCYGHAAIDT
                     LLADMAASAQPIHCLVSDPQLTARVEAWLGQPMAPGTTESRGNLHVLRLPFLSQDDYD
                     RLLWLCDLNFVRGEDSWLRALWAGKPAIWQPYRQEEDAHLAKLEAYLVGKDADLEDEL
                     RAELQAIHRAWAEGRWHAGSIAWLQRNLAALQAHAEHYCHRLARQPDLATKLVIFCRN
                     LF"
     gene            3775..>4097
                     /locus_tag="VN084_06585"
     CDS             3775..>4097
                     /locus_tag="VN084_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWT29162.1"
                     /translation="MKTAQELRVGNVFMVGNDPMVVLKAEYSKSGRNSSVVKMKFKNL
                     LTESPSEAVYKADDKFDVVVLEKKEVTYSYFADPMYVFMDAEYNQYEVEAETMEDALN
                     FLEDG"
BASE COUNT          848 a         1255 c         1183 g          811 t
ORIGIN      
        1 cttatggcag accaaaagaa ctacatcacc cccgaaggat tcgccacttt ggaagcggaa
       61 ttccaccagc tactgaaagt ggaacgcccg gaagtggtcc gtaccgtggc ctgggccgcc
      121 agcaatggcg atcgctcgga gaacggcgac tatatctatg gcaagaagcg tctgcggcag
      181 atcgactcgc gcctgcgttt cctcatgaaa cgcatggaca atgccgaagt ggtcgacccc
      241 aagacccagc aaggcctgga aaaggtctat tttggcgcct gggtgacgct gtattcgctc
      301 gatcgcgaca ccgagcacac ctaccgcatc gtcggccagg acgaacttga accgagcaag
      361 ggctatatca gctgggtctc gccgctggcg cgcagcatgc tcggcaagca ggtgggcgat
      421 gtggtcaggg tgcagacgcc gggaggcgaa gaggcttacg agatcacggc catcgattac
      481 ctggcggcgg aacattgatc gggtgaacta atgccagcca ctgccctctc atatctcatg
      541 agaaaattcc tctgcctgct actggtacta tggctgccgt tatttaccgg ctaggcctgg
      601 gccatgcgca cgcagatgca gctcgatcag ctgcaaggcg cggcagaaac gcacatgctc
      661 gccagccacc cttgccatga catgccgggc atgcaggtca gcgggcatga cgatcatgcg
      721 acccacggcg ccatggccat gcatgacatg gagaagccgg gtgccaccga cgccggcaag
      781 caagaccgca acggtcattg ccaaggccac gattgcttcg cctgcggaat atgtctgcat
      841 gcgacgaatt tcaccctcac cgtcatgcca cccatgccgg caccgatgct ttcccatgcc
      901 aagcccgtca cacacgagac ggtcttcgct tccctgcgct atccacccgc attgaaaccg
      961 ccgatcgacg cctgaacccg ctttagtggt ttaacgtttt ttcggaggaa tcatgaatcg
     1021 caacaatctg gcgacgctcg ccctgctggc gctggcgttg cctttgcccg gccatgcagc
     1081 tgcgctgatg ggctttgccg acagcacgat gttcatgggt gaatggagcg ccgattatca
     1141 agagatcatg gccaactatt cgcccagctt cggtcatgcc atcggtatcg aatccatgca
     1201 gatgcgcgac aaggatggca tgcgcagcgg catcgccgcc atcaattaca ccggcatctt
     1261 caagcgctgg aacctgccac aaggccaagc caacctctgg ttcaccggcg gtatcggcga
     1321 atcctacggc agtgaccggc aaggctttgc ctatacgcca ggcctgcaat tcgactatga
     1381 gacgacgcgc gtctatttcc tggccagagc caggatgatg cgcgcaccgg gcatgaactt
     1441 cgataccgct gcagtgcagg gcgggttctc gttctacgag accagctacg agcacaccca
     1501 gccctggttc gtagtcgagg cacgcaccat gcgcaatatg gacccggcga tgcaggtcac
     1561 gccggctcta cgcttgatca acaaagacta ttttctcgaa ctcgggctca ccaacccttg
     1621 ggatgagggg gtgcgcatgc cgcgcatcaa cttcatgctg acgttctagt gcccattcaa
     1681 tcaaggatcc aatcatgaaa aagatcgtaa tgttaggtat gacaatcgca ttctccaccc
     1741 tgatgttctc gtccagcgcg ttcgccacat cgagcatcaa agccaaggtc aacggcatgg
     1801 tctgcgcgtt ctgcgccaag ggcatcgaaa agaagctcaa ggccttgcca cagacccagt
     1861 ccgtgttcgt cgacctcaag gcacgggtgg tggcgctgga gctgaaagac ggccaaacgg
     1921 tgccgatcga ggatttccgc aagatcatcc gcgatgccgg ctatgaggtg gctgaagccg
     1981 aggtcgtcga tcgttcggca gaccagatca aggccgaggt gacgcaatga gcacgatcga
     2041 gccgctagcg accgatactg cgctggccgc cagcagaagg ctgaatgtgt tctcgctgtt
     2101 caccagcttc tccacattga tctgctgcgc gctgccggcg ctgctggtcg cactgggcgc
     2161 cggggccgca ttgtcgaccc tggtcagcaa cgtgccgcag cttgtctgga tcagcgatca
     2221 caagccggaa gtgttcggcg cggcgggctt gatgctactg gtcgcaggct acttccagtg
     2281 gcgcgcccgg catgcgccct gccctgccga cccggcgctg gcggcggttt gcagacgcgc
     2341 gcgcaggaac gcacgctgga cctactggat ctccgtcggc atctatgccg tcggtgcctg
     2401 gttcgctttc gttgcgccat tactgctttg aggctgtagt tccaaggcaa gcccggtcgc
     2461 acgctgtcgc gaccgggcga tcggcgcgcc tgccatgatt gatttaaact gcacgcatga
     2521 ctgcgccgga caagccacaa cgctgggata tcttttgccg catcgtcgac aattacggcg
     2581 atatcggcgt ctgctggcgc ctggcacgcc aactcacgga tgaccacggc atcagggtcc
     2641 gcctctgggt cgacgacctg gcggtggcgc aacggctgat ccccgcgatc gacttgcagc
     2701 tgcccagcca gcgcatcgaa gacgtcgaga tcgcccactg ggtggccgat tttccggata
     2761 cgacggtggc agatgtcgtc attgaggctt tcgcatgcga actcccgccg cgttatctca
     2821 acgctatggc cagcgccaga ccggtatggc tcaatctcga atacctgtcg gccgaatcct
     2881 gggtcgctga tttccatctc agaccttcgc cgcatccacg cctgccgctg accaaatact
     2941 tctattttcc gggatttgtg gaggagtccg gcggcttgct gcgcgaacgg ggcttgatca
     3001 cagcccgcga ggccttccag cgatcgccgc aggcccaggc cgcattctgc cggcgcctcg
     3061 gcatcgatag gcttcggagc gccacttccg gtgatcgcga cagcgcaaat tcagacagca
     3121 tgaccatgcg ggtatcgctc ttttgttacg gtcatgctgc gatcgacacg ctgctggcgg
     3181 acatggccgc gtcggcgcaa cccatccatt gcctggtcag cgacccgcaa ttgacggcgc
     3241 gggtcgaggc atggcttggc cagccgatgg ctcccggcac caccgaaagt cgcggcaact
     3301 tgcatgtgct acgcctgccc ttcctcagcc aggacgatta tgaccgcctg ctgtggctgt
     3361 gcgacctcaa tttcgtgcgt ggcgaggata gctggctacg cgccctgtgg gcgggcaagc
     3421 ccgccatctg gcaaccctac cgccaagagg aggatgccca tctagccaag ttggaggctt
     3481 atctcgtcgg caaagacgcc gatctcgaag acgaactgcg tgccgaactg caggctatcc
     3541 atcgcgcatg ggcagaaggt cgctggcatg ccgggagcat cgcctggtta cagcggaatc
     3601 tggccgcgct gcaggcgcat gcggaacact attgccatcg cctggcgcga cagccggacc
     3661 tcgccactaa gcttgtgatt ttctgcagga atcttttttg atcttcaggt ataattcgcg
     3721 actttttacc gctaacccct tcaagcagca cccttattca gagatcgcca cgatatgaaa
     3781 acagctcagg aactccgcgt aggtaacgtg tttatggtgg gcaacgaccc aatggtcgtg
     3841 ctcaaggccg agtacagcaa atccggtcgc aactcttccg tcgtcaagat gaagttcaag
     3901 aatctcttga ccgagtcgcc gagcgaagcc gtttacaagg ccgacgacaa gttcgacgtg
     3961 gtcgtgctgg aaaagaagga agtcacttac tcctatttcg ctgacccgat gtatgttttc
     4021 atggatgccg agtacaacca gtacgaagta gaagctgaaa ccatggaaga cgcgctgaac
     4081 ttcctggaag acgggat
//
LOCUS       DATMTW010000336         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_29620, whole genome shotgun sequence.
ACCESSION   DATMTW010000336 DATMTW010000000
VERSION     DATMTW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29620"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN084_06590"
     CDS             complement(<1..207)
                     /locus_tag="VN084_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfG"
                     /protein_id="HWT29163.1"
                     /translation="MDRTIIRHAVVTALILTAFTVVGTALLSGTFEATKSPIADSERQ
                     ATLHLLSQVMPASLHDNDLLADSLD"
     gene            complement(223..1242)
                     /gene="rsxD"
                     /locus_tag="VN084_06595"
     CDS             complement(223..1242)
                     /gene="rsxD"
                     /locus_tag="VN084_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HWT29164.1"
                     /translation="MNRSPYLVEKTSVSIIMLKVLLALIPGIVAYVWIYGTGILVTLT
                     IASVTALASEAIVLKLRQRPVLPFLLDGSALVTAWLLALALPPLAPWWLIVVGTLFAI
                     VVAKQLYGGLGYNPFNPAMVGYAVLLISFPLLMTQWPAPAVLAQAHLGFMDQLAYIFG
                     RHLPAGVALDAISSATPLDYLKTQLMLQHPVADITQSSIFGFMGAKGGELVTAAYLLG
                     GLYLWQQRVISWHIPVAFLGTLAVMAGVFQWADATHFASPQFHLFSGATMLCAFFIAT
                     DPVSGPTTPKGKLIFAAGIAVITLLIRVYGGYPDGVAFAVLLMNLCVPLIDMYTQPRV
                     FGHEQ"
     gene            complement(1293..3056)
                     /gene="rsxC"
                     /locus_tag="VN084_06600"
     CDS             complement(1293..3056)
                     /gene="rsxC"
                     /locus_tag="VN084_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HWT29165.1"
                     /translation="MNAITELSKLFKFNGGVHPPENKLQSCATPIRKLPMPAKLVLPL
                     RQHVGNLPKLRVAVGEQVHKGQLLAEAEGTISAAVHAPTSGRVASIGDALIPHPSGLP
                     DVCITLEPDGLDEWLPHTPPDWRTADKQQLVASLRSSGIVGLGGAAFPSHIKLRADGS
                     SGIEILVINGAECEPFITCDDLLMRERAEEVVRGIEIAYYLLGAKQCLIGIEDNKPEA
                     AAAMRAACEKSEYPLQVVVVPTIYPSGDARRLIHVLTGREIPSFKRSTDMGIQCFNVG
                     TVQAIYRYFQYGEPALSRIVTVTGNVAAPGNFEVLLGTPVTTLLAAAGGALPDTDDYL
                     MGGPMMGFSLPSAEVPVTKATNCIIAGAPKLFPAPEPALPCIRCARCADACPVNLQPQ
                     ELYWFAKSSNLEKARDYKLFDCIECGCCSYVCPSHIPLVQYYRFAKSEIIAQDRAKQA
                     ADVARERNEFRLMRIEREKQERAQKHAQKAAGAKTEGAAGEGKTLEDAKKAAIEAAVA
                     RAQAQKAAVEPQNVSDLPPAVQSEIAEIDARRSEAALQVEKRQLQEAIGTNQANAAHQ
                     APDEQSVAERATPGSTPTTEQ"
     gene            complement(3053..3631)
                     /gene="rsxB"
                     /locus_tag="VN084_06605"
     CDS             complement(3053..3631)
                     /gene="rsxB"
                     /locus_tag="VN084_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HWT29166.1"
                     /translation="MLTALLVMVGLALILGLLLGYSALKFKVEGDPLVDRIDAILPQT
                     QCGQCGFPGCKPYATAIAKGEADINQCPPGGDAGVHALADLLGVEYKPLNAEHGLPKP
                     KSVAFIDENTCIGCTLCIQACPVDAILGAAKHMHTIIASECTGCELCLAPCPVDCITM
                     EPIAETPDNWKWRYPVFAIQPAAPEIKDSKAA"
     gene            complement(3631..>4158)
                     /gene="rsxA"
                     /locus_tag="VN084_06610"
     CDS             complement(3631..>4158)
                     /gene="rsxA"
                     /locus_tag="VN084_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HWT29167.1"
                     /translation="IVLIKVLGLCPFMGVSKKLEASIAMGLATAFVLTVGSGTSYLIS
                     EYLLGTDLFYLRTLSFIVVIAAVVQLTEMWMEKNSPVLYQVLGIYLPLITTNCAVLGV
                     PLLNVQADHNLVESLLFGLGGAVGFSMVLIIFASIRERLEAADVPVIFRGSAIAFVTA
                     GLMSLAFMGFAGLVR"
BASE COUNT          815 a         1295 c         1295 g          753 t
ORIGIN      
        1 gtccaggcta tcggccagca ggtcgttgtc atgcaggctg gccggcatca cctgcgacaa
       61 cagatgcagt gttgcctgcc gctcgctgtc tgcgatcggc gatttggtcg cttcgaaggt
      121 gccggacagc agcgcggtcc cgacgaccgt gaatgccgtc aggatcaagg cggtgacgac
      181 agcatgtctg atgatggttc tatccatggc ggtctagtcg actcactgtt cgtgcccgaa
      241 gactcggggc tgggtgtaca tgtcgatcag cggcacgcaa aggttcatca gcagcacggc
      301 aaacgccacg ccatccggat agccgccata aacgcggatc aacagggtga tgacggcgat
      361 gccggcagcg aagatgagct tgcctttagg cgtcgtcggg ccactgaccg gatcggtggc
      421 gatgaagaac gcgcagagca tggtggcgcc actgaacagg tggaactgcg ggctggcgaa
      481 atgcgtcgcg tcagcccact ggaagacacc tgccatcact gccagcgtgc cgaggaaggc
      541 gaccgggata tgccagctga tgacgcgttg ctgccacaga tacaagccac ccagcagata
      601 ggctgccgtc acgagctcgc cgcccttggc gcccatgaag ccgaagatcg acgattgcgt
      661 gatgtcggcc acgggatgct gcagcatgag ttgcgtcttc aggtagtcga gcggcgtcgc
      721 cgaactgatg gcgtccagcg ccacgccagc cggcaggtgg cggccgaaga tataggccag
      781 ctgatccata aagcccagat gtgcctgggc cagcacggcg ggcgcaggcc attgcgtcat
      841 cagcaacggg aacgagatca gcagcacggc ataaccgacc atcgccgggt tgaatgggtt
      901 atagccaagc ccgccataga gttgcttggc gaccacgatc gcgaacaagg tgccgacgac
      961 gatcagccac caaggcgcca gtggcggcaa ggccaacgcc agcagccagg cggtgaccaa
     1021 ggcactgcca tcgagcagga atggcagcac ggggcgctgc cgcaacttca atacgatcgc
     1081 ttcgctggca agcgctgtca ccgaagcgat cgtcagcgtg accaggatac cggtgccgta
     1141 tatccagaca taggcgacga tgcccgggat caaggcgagc aggaccttca gcatgatgat
     1201 gctgacactg gttttctcta ccaaataggg cgatctgttc atcttcttct cgtctctacc
     1261 cgacccgccg cgaggcggca ggcttggcca gtttattgtt cagtggtcgg cgttgagccg
     1321 ggtgttgcgc gctcggctac ggactgttca tccggagcct ggtgggcggc attggcctgg
     1381 ttggtaccga tcgcttcctg cagttgccgc ttttccactt gcagggcggc ctcgctgcgc
     1441 ctggcgtcga tctcggcgat ctcactctgc accgctggcg gcaagtcgct gacgttctgt
     1501 ggctcgacag cggccttctg cgcctgcgcg cgggcgaccg cggcctcgat agccgctttc
     1561 ttcgcgtctt ccagggtctt gccttcgcca gcggcacctt ccgtcttggc tccggccgct
     1621 ttctgcgcat gcttctgcgc gcgctcctgc ttctcgcgct cgatccgcat caggcggaac
     1681 tcgttacgct cgcgagcgac atctgccgcc tgtttggcgc gatcctgagc gatgatctcg
     1741 ctcttggcga aacggtaata ctgcaccagc gggatatggc tgggacacac ataactgcag
     1801 catccgcact cgatgcaatc gaacagcttg taatcgcgcg ccttctccag attactggac
     1861 ttggcgaacc agtagagctc ctgcggctgc aggttgaccg gacaagcatc cgcgcaacgc
     1921 gcgcagcgga tgcatggcag ggccggttca ggcgccggaa acaacttggg tgcaccggcg
     1981 atgatgcaat tggtggcctt ggtcaccggc acttcggccg agggcagcga aaaacccatc
     2041 atcggcccgc ccatcaggta atcgtcggta tccggcaagg cgccgcctgc cgctgccagc
     2101 agcgtcgtca ccggcgtgcc gagcaacact tcgaaattgc ccggcgcagc gacattgccc
     2161 gtgaccgtga cgatgcgcga caaggctggc tcgccatact ggaagtaacg gtagatggcc
     2221 tgcaccgtgc cgacgttgaa gcactggata cccatgtcgg tcgatcgctt gaagctcggg
     2281 atctcccgac ccgtcagcac gtggatcaac cgacgcgcat ccccgcttgg atagatcgtc
     2341 ggcacgacga ccacctgcag tggatattcg ctcttttcgc aggctgcgcg catcgcagcg
     2401 gctgcctccg gcttgttatc ctcgatgccg atcaggcact gtttcgcccc gagcaggtaa
     2461 taggcgatct cgataccccg caccacctct tcggcacgct cgcgcatgag caaatcgtca
     2521 caggtgatga agggctcgca ctcggcgccg ttgatcacca ggatctcgat gcctgaactg
     2581 ccgtccgcgc gcaacttgat gtggctgggg aatgccgccc cgccaagtcc gacgataccg
     2641 gagctgcgca ggctggcgac cagctgctgc ttgtcggctg tgcgccagtc cggcggcgta
     2701 tgcggcaacc attcgtccag gccgtccggc tccaatgtga tacagacgtc tggcagaccc
     2761 gacgggtgcg ggatcagcgc atcgccgatg ctggcgacac ggcccgaggt cggcgcgtgt
     2821 acggcagccg agatcgtgcc ctcggcttcg gccagcaact gccccttgtg cacctgttcg
     2881 ccgaccgcca cacgcagctt gggcagatta ccgacatgct gacgcaaggg cagaaccagc
     2941 tttgccggca tcggcaactt gcggatgggc gtcgcgcagg actgcagctt gttctcgggc
     3001 ggatgtacgc cgccgttgaa cttgaacagt ttcgataatt cggtgatcgc gttcatgccg
     3061 ctttcgagtc cttgatctca ggtgctgcag gctggatggc gaacaccggg tagcgccact
     3121 tccagttatc cggcgtctcg gcgatcggct ccatggtgat gcaatccacc gggcacggcg
     3181 cgaggcacaa ctcgcagccc gtacattcgg aggcgatgat ggtgtgcata tgcttggccg
     3241 cgccgagaat ggcgtctacc gggcaggcct ggatacacag cgtgcaaccg atgcaggtgt
     3301 tttcgtcgat gaacgccacc gacttcggct tgggcagccc atgctccgcg ttcagcggct
     3361 tatattccac gccgagcagg tcggccagcg cgtgcacgcc ggcatcaccc cccggggggc
     3421 actggttgat atctgcctcg cccttggcga tcgccgtcgc atagggcttg catccgggga
     3481 aaccgcactg gccgcactgt gtctgcggca ggatggcgtc gatacgatcg accagcggat
     3541 ccccctcgac cttgaactta agggccgaat aaccaagcaa caaccccagg atgagggcga
     3601 gcccgaccat caccagcaag gccgtcaaca ttagcgcacc aacccggcaa agcccatgaa
     3661 ggcgaggctc atcaaccctg cggtgacgaa cgcgatggct gaaccgcgaa agatcacggg
     3721 tacgtcggcg gcttccaggc gttcacggat ggacgcgaag atgatcagca ccatcgaaaa
     3781 accgaccgcc ccacccaggc cgaacagcaa ggactcgaca aggttgtggt ccgcctgcac
     3841 gttgagcagc ggcacgccca acacggcaca gttggtggtg atcagcggca gatagatgcc
     3901 gagcacctga tagagcacgg gggaattctt ctccatccac atttctgtga gctggacgac
     3961 agccgcaatg acgacgatga aagacaatgt gcgcagatag aacaggtcgg tgcccagcag
     4021 gtattcgctg atgaggtaac tggtaccgga gccgacggtg agtacgaagg cggtggccag
     4081 ccccatggcg atagaggctt ccagcttctt ggagaccccc atgaacgggc agagaccgag
     4141 caccttgatc aacacgat
//
LOCUS       DATMTW010000337         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_29827, whole genome shotgun sequence.
ACCESSION   DATMTW010000337 DATMTW010000000
VERSION     DATMTW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29827"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..3358)
                     /locus_tag="VN084_06615"
     CDS             complement(215..3358)
                     /locus_tag="VN084_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT29168.1"
                     /translation="MDLKWLDSLRVRLILLICLSSIPPLVVVIYNAVYEGERIESDAL
                     ENAYKLAQLASQYQGGLVEGTQELLVGISHTPAVIAGDWPKCERYLTNVLGKNTRYSN
                     LGVANLKGDILCSGEPMTRKLNVRDRQYFIRALATGSFAVGDYHVGRVTGIPSLGMAL
                     PVYEADGRIVAIAYAAINLETLKQTVDEIELPTDGILFMFDRNGKLLVQSRQQGSHWV
                     LDHAREIWARAAPGASANAEIYRAQDDMSWIFATSRLRGILDRDAISIVIATPMESML
                     KESRRYYFLVLGIMFFSALLVFAISLFGVQAFVLRRIQTLIEATKAIGQGQFGLQVQP
                     RVKGGELGQLAHHIDDMADKIQRSLVREQQYRYLFERNPNPMWIFARAEFAYLAVNEA
                     AIKHYGYSRDEFLRMGVTDILPRNELQRAQSFVEAIRENELRTDTWRHLKKNGEEILV
                     EVATYPIDFEDTPAIIASIKDITHETQVRARLADRDEQIRSMLESTAEAICGVDLQGR
                     CTFANPALARLLGYDDPEALAGRDMLALMQPGRPADEGLPTEDSEIFRALQAGRRAHS
                     EEEIFSRSDGSTFPVEYWSYPIYRDDQVSGAVITFIDITERKLQQQALEFQAQHDSLT
                     GLPNRRMLNQRLQELCAKPNKSFHLMMIDLDRFKEVNDALGHHAGDLLLMQIASRLKQ
                     WLRRGYFLARLGGDEFAIIMPSLPRNMTAIDLAQDLVSLIEQPVEITGIRVQIGASIG
                     ITAFPEDGEQSGGLLRCADVAMYSAKRSGAMVAMYDSTSDINTAEHLALMSDLHQAIE
                     QNQLTLHFQPKIRLRDRSIDSFEALIRWNHPQKGMIPPSQFIPLIELTSLIRPFTAWV
                     LEHAMRTCREWRAHGLNAGVAVNVSTRNLLDMALPGQIDDLLANYALSADYLELEITE
                     SAIMSDPVRSRDVLARIRESGVHVSIDDFGTGYSSLEYLQKLPINAIKIDQTFIRQLL
                     HQQEAISIVRAVIGLAHSLKIEVVAEGVEDEPTMEILNDLGCDIIQGYFISRPLGVEE
                     LLGWIDERIGRDAATGR"
     gene            3590..4996
                     /locus_tag="VN084_06620"
     CDS             3590..4996
                     /locus_tag="VN084_06620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWT29169.1"
                     /translation="MNQPLTQQEQLRLAEIHRYEILDTPPENDFDDLVKLAALIFKVP
                     ISTVTIVDEHRQWFKAAVGLDVPETPRDISFCTHAIELEQPLVVEDTAKDPRFSANPL
                     VLGAPHLGFYAGIPLRTSENYAIGTFCIMDQAPRQFSKAELEILRVLANQAMKLLELR
                     AEKIKLRESEQRWKFALEGAGDGVWDWNIQTGETVFSPQWKAMLGYADAELENSYETW
                     ESLVHPDDRSPLRSQLEDYLHGKAENYRIEHRLRCKNGEWKWILARGMVVARDAHGKP
                     LRMVGTHTDISVRKASEELIWRQANFDALTSLPNRRMFFDRLKEAIKRAARNRHTFAL
                     LFIDLDGFKQVNDTLGHQAGDKLLIQVSKRIAATIRASDTLSRLAGDEFTVILSDIDH
                     IDQVGKIADKILAQLNRPFKIGKSHASISGSIGISLYPMDGVDADTLINAADTAMYAA
                     KEQGKHCWVHLPTTQAVS"
     gene            complement(5127..5693)
                     /locus_tag="VN084_06625"
     CDS             complement(5127..5693)
                     /locus_tag="VN084_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HWT29170.1"
                     /translation="MASLTRIIYISRSTFAPVGNGSDIEPNVARILAKSRVNNRKNGL
                     VGVLYFGDGNFFQCLEGESSAVDTLYAKLQKDPRHKDLKLLSRKSIQKLSFIDWAMKY
                     VPLEVQMNQLLKQHGHASFDPYKFDAEMVRQVMLLLHANQQADDGAEDGPEFNRKQRM
                     QFMLNLAAVIMSALALVLSATALAVALS"
     gene            complement(5716..6024)
                     /locus_tag="VN084_06630"
     CDS             complement(5716..6024)
                     /locus_tag="VN084_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29171.1"
                     /translation="MKEQFQPAQISKVIDQALVYQCACPAQVCRTIFELRELYEYQMN
                     CANDTINDQLVHNTIAAAAEQAHALMEECLKQILEIEGWDPENLVMPDALRKKPAKTL
                     "
     gene            complement(6021..7055)
                     /locus_tag="VN084_06635"
     CDS             complement(6021..7055)
                     /locus_tag="VN084_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWT29172.1"
                     /translation="MMQTPRLLLVDSDSYTSSVILDDLQRRNITDVQHVAHALALPRV
                     LQTTKPDVVIFNYHSQYPDSLIACSTIKLISPHSAIIAIVSPGPALKAVRELAKQAEC
                     IDIIIEKPLSDERFYMAVEDLLKVKLVTRELENRADKLAQLVPEGALTALDNEFDSEA
                     ELFEAAVLFTDIRGSSQLITEMPPRDFFETLNRLLSAQAKKIQQYEGSVIKYTGDGVM
                     AIFKGMGRSYLALRCGLELAEDSKDQRLACGIGITEGLVLAGLIGDSHHAGQRRQYDV
                     IGANVHLAARLCSMANAGEVITTKRVNSIAHLRNPEPRQIGGISIRGFDNEIDCVAFD
                     LNSSPRRLLK"
BASE COUNT         1486 a         2161 c         2167 g         1524 t
ORIGIN      
        1 gagcaaaaac cgcgccagtg ctcatgcgcc tgtttcatat acggtttttt gatgcaccat
       61 tttggaaacc gcactttaac tgtgcatatc aaagtgcatt gcactattaa cgtgcgtttt
      121 ggctttgccg tgagggcact gagtcgctag ccgcctgcct gcgtcccgac tggaccgctc
      181 tggcgtcgct cgcgaccgag ctggcgacac agccctagcg accggtcgcg gcatcgcgac
      241 cgatacgctc gtcgatccag ccgaggagtt cttccacacc caagggccgg ctgatgaaat
      301 acccctggat gatgtcgcag ccgaggtcat tgaggatctc catcgtcggt tcgtcttcca
      361 caccttccgc gacgacttcg atcttcaggc tatgggcgag gccgatcacg gcgcgcacga
      421 tcgagatggc ctcctgctgg tgcagcaatt gacggatgaa ggtctggtcg atcttgatgg
      481 cattgatggg gagcttctgc aagtactcca gcgaggaata gccggtaccg aaatcgtcga
      541 tcgagacgtg caccccactc tcgcggatac gggcgaggac gtcgcgcgag cgcacagggt
      601 cggacatgat ggcgctctcg gtgatctcca gctcgaggta gtctgccgat agcgcgtaat
      661 tcgccagcag gtcgtcgatc tgcccaggca aggccatgtc gaggaggttg cgtgtggaga
      721 cgttgaccgc gacgccggcg ttcaggccat gtgcacgcca ttcccggcag gtgcgcatgg
      781 catgctccaa cacccaggcg gtgaatggac ggataagcga tgtcaactcg atcagcggga
      841 tgaactggct gggcgggatc atgcccttct gcggatggtt ccagcggatc agcgcctcaa
      901 agctgtcgat gctgcggtcg cgcaggcgga tcttgggctg aaaatgcagg gtcaactgat
      961 tctgctcgat ggcttggtgc aggtcggaca tcagtgccag gtgctcggcc gtattgatat
     1021 ccgaggtact gtcatacatg gctaccatgg cccccgaacg tttcgcgctg tacatggcga
     1081 catcggcgca gcgcaacagc ccaccggact gctcgccatc ctccgggaaa gcggtgatgc
     1141 cgatactggc gccgatctga acgcggatac ccgtgatctc gaccggctgt tcgatcaggc
     1201 tgaccaggtc ctgcgcgagg tcgatggcgg tcatgttgcg aggcaggctg ggcatgatga
     1261 tggcgaactc gtccccgccc aggcgtgcca ggaaatagcc gcggcgcagc cactgcttga
     1321 gccttgacgc gatctgcatg agcagcaagt cgccggcatg atggcccagt gcatcgttga
     1381 cttccttgaa gcgatcgaga tcaatcatca tcaggtggaa actcttgttg ggcttggcac
     1441 atagctcctg caggcgctgg ttcagcatcc tgcggttagg caggccagtg agcgaatcgt
     1501 gctgcgcctg gaactccagc gcctgctgct gcagcttgcg ctcggtgata tcgatgaagg
     1561 tgatgaccgc gccactgacc tgatcgtcac gatagatcgg atacgaccag tattcgaccg
     1621 ggaaggtgct gccgtcgctg cgcgagaaga tctcctcctc cgaatgcgcc cttcgaccgg
     1681 cctgcagcgc acggaatatc tcgctatcct cggtcggcag accctcatca gcgggtctac
     1741 cgggctgcat cagtgccagc atatccctgc cggccagcgc ttccggatcg tcatagccca
     1801 acaggcgcgc cagcgccggg ttggcgaacg tacagcgccc ctgcaggtcg accccgcaaa
     1861 tggcttccgc cgtcgattcg agcatcgagc ggatctgctc gtccctgtcc gcgagtcggg
     1921 cccttacttg cgtctcgtgc gtgatgtcct tgatcgaggc gatgatcgca ggcgtgtcct
     1981 cgaaatcgat cgggtaggtc gcgacctcga ccaggatctc ctccccgttc tttttcaggt
     2041 gtcgccaggt atccgtgcgc agctcattct cccggatcgc ctcgacgaag gattgcgcgc
     2101 gctgcaactc gttcctgggc aggatgtctg tcacacccat gcgcaggaac tcgtcgcggc
     2161 tgtagccata atgcttgatg gcggcctcat tgaccgccag gtatgcgaac tccgcgcgcg
     2221 cgaatatcca catcggattg ggattgcgct cgaacaggta ccggtattgc tgctcgcgca
     2281 ccagcgagcg ctggatcttg tcagccatgt cgtcgatgtg atgggccagc tgccccaatt
     2341 cgccgccctt cacccgtggc tgcacttgca ggccgaactg accctggccg atggccttgg
     2401 tcgcctcgat gagagtctgg atgcgcctca gaacaaaagc ctgtacgccg aacagcgaga
     2461 tcgcgaacac cagcaaggcg ctgaagaaca tgatgccgag cacgaggaaa taatagcgtc
     2521 gcgattcctt cagcatcgat tccatcggcg tggcgatgac gatggagatc gcgtccctgt
     2581 caaggatgcc gcgcaagcgt gaagtggcga agatccacga catgtcgtct tgcgcccggt
     2641 agatctcggc attggccgac gcgccaggcg cagcccgtgc ccagatctcc cgggcatgat
     2701 ccaagaccca gtgcgacccc tgctgacgcg actgcacgag cagcttgccg ttacggtcga
     2761 acatgaagag gatcccgtcc gtgggcagct cgatctcatc gaccgtttgt ttcaatgttt
     2821 cgagattgat ggcggcatac gcgatggcga cgatgcggcc atcggcttca tagactggca
     2881 acgccatgcc caggctgggg atgccggtga cacgaccgac gtgataatcg cccacggcaa
     2941 agctgcccgt tgcgagcgcg cggatgaaat actggcggtc cctgacgttc agcttgcgcg
     3001 tcatgggctc gccgctgcag aggatatcgc ccttcaggtt cgccacgccg agattgctgt
     3061 agcgcgtgtt cttaccgagc acgttggtga ggtaacgctc gcatttcggc caatcaccgg
     3121 cgatcaccgc cggcgtatgg ctgataccga ccagcaattc ctgtgtccct tcgaccaggc
     3181 cgccctggta ttgggaagcg agctgggcga gtttgtaagc gttctccagc gcatcggatt
     3241 cgatacgttc gccttcatag acggcgttgt agatgacgac gaccagtggc gggatggatg
     3301 acaggcagat cagtaaaatc aggcgaacgc gtaagctatc gagccatttg agatccaaaa
     3361 cgaattctcc gtctccccga tgtcatgtag gttgttttta tttttcggtc tcttgcacgc
     3421 gaggcctatt caacatttat ccatagttcg attatttagt aaacgcctgc aaagatggca
     3481 ctaccttaga ttgtcagtag gtgccgaaat caattcgcgg atacggtaac tttcgctgcg
     3541 gatttacgtc atactgcata tgcaaactcg tgcgcaacaa ggtattgcca tgaaccaacc
     3601 gctcactcag caagaacagc tacgcctggc ggaaatccat cgctacgaga tcctcgacac
     3661 cccaccggaa aacgattttg acgacctggt caaactcgct gcgctgatct tcaaggtccc
     3721 catctccacc gtcaccatcg tcgatgaaca tcgccaatgg ttcaaggccg cggtcggcct
     3781 cgacgtgcca gagacgcctc gcgacatttc attctgcacg catgcgatcg agctggagca
     3841 accgctggta gtggaagaca ccgccaaaga tccacgtttc tcggcgaacc cgctggtgct
     3901 cggcgcaccg cacctggggt tttatgccgg gatcccgcta cgcaccagcg agaactacgc
     3961 catcggcacc ttctgcatca tggatcaggc gccgcgacag ttcagcaagg cagagcttga
     4021 gatcctgcgc gtcctggcca atcaggccat gaagctactg gagctgcgcg ccgagaagat
     4081 caagctgcgc gagagcgaac aacgctggaa attcgcgctg gaaggcgcag gcgacggggt
     4141 ttgggactgg aacatccaga ccggcgaaac cgtcttcagt ccacagtgga aagccatgct
     4201 cggctatgcg gacgcggagc ttgaaaacag ttacgagacc tgggaatcgc ttgtgcatcc
     4261 agacgacagg agccccctac ggtcacaact ggaggattac ctgcacggca aagccgagaa
     4321 ctaccgtatc gagcatcgcc tgcgctgcaa gaatggcgaa tggaaatgga tcctggcgcg
     4381 cggcatggtg gtcgcgcgtg atgcgcacgg caagccgctg cgtatggtcg gcacgcatac
     4441 tgacatctct gtccgcaagg cctcggaaga gctaatctgg cgccaggcca acttcgatgc
     4501 gcttaccagc ctgcccaatc gccgcatgtt cttcgaccgg ctgaaggagg cgatcaagcg
     4561 cgcggcacgc aaccggcata ccttcgcgtt actgttcatc gacctcgacg gcttcaagca
     4621 ggtcaacgac acgctggggc atcaggctgg cgacaagcta ctgatacagg tcagcaaacg
     4681 catcgccgcg accattcgcg ccagcgacac cttgtcacga ctggcaggcg acgaattcac
     4741 cgtcatcctg agcgacatcg atcacatcga tcaggtcggc aagatcgccg acaagatcct
     4801 cgcccagctc aaccgcccgt tcaagatcgg taaatcgcat gccagcatct ccggcagcat
     4861 cggcatctcg ctctacccga tggatggtgt agatgcggat acgctcatca atgccgctga
     4921 tacggcgatg tacgccgcca aggaacaggg caaacactgc tgggtacatc tgccgacaac
     4981 acaagctgta agctgaatcg gccggctgca agcacaggca tcagcgaagt ctgccggcac
     5041 gctggctacg ccgcaaccga tccgctcggg tcgatcgcat cgtgtagcat tgcgccgcct
     5101 ggcattacac ttcctggcat cacaccctag ctgagagcga ccgccagggc tgttgcagat
     5161 agcaccaacg ccagcgcaga catgatcacg gcagcgaggt tgagcatgaa ctgcatgcgt
     5221 tgcttgcgat tgaattccgg cccgtcttcc gcaccatcgt ccgcctgctg gttggcatgc
     5281 agcaataaca tcacttgacg caccatctcg gcatcgaact tataggggtc gaagctcgca
     5341 tggccgtgct gcttgagtag ctggttcatc tgcacctcaa gcggcacata cttcatcgcc
     5401 cagtcgatga acgacagctt ctggatggat ttgcgcgaca gcagcttgag atccttgtga
     5461 cgcggatcct tctgcagttt ggcgtacaag gtatcgaccg cggacgattc gccttccagg
     5521 cactggaaga aattaccgtc accgaaatag agcacgccga ccaggccgtt cttgcggttg
     5581 ttgacgcggg attttgcaag gatgcgggcg acgttgggtt cgatatcgct gccgttgccg
     5641 accggggcaa aggtcgagcg actgatgtag atgatgcggg tcaatgaggc catgggatta
     5701 ccttgtcagt gggcgttaga gggttttggc gggttttttc ctgagggcat ccggcatgac
     5761 cagattttcg ggatcccaac cctcgatctc caggatctgc ttcaggcatt cctccatcaa
     5821 ggcatgcgcc tgctcggcgg cggcggcgat ggtgttgtgc acaagctggt cattgatggt
     5881 gtcgttcgcg caattcatct ggtactcata cagctcgcgc aactcgaaga tggtgcggca
     5941 gacctgagct ggacaggcgc actggtagac cagcgcctgg tcgatcacct tgctgatctg
     6001 ggcgggttgg aattgttctt tcatttaagc agtctcctgg gggatgagtt gaggtcgaaa
     6061 gcgacgcaat cgatttcgtt gtcgaagcct cgaatggaaa taccgccgat ctgtctcggc
     6121 tcggggttgc gcagatgcgc gatactgttg acgcgcttgg tggtgatgac ttcgccggcg
     6181 ttcgccatgc tacacagccg cgcggccaga tggacattgg caccgatgac gtcgtactgc
     6241 cggcgctggc cagcatggtg cgaatcaccg atcaacccgg ccagcaccag gccttcggtg
     6301 atgccgatgc cacaggctag tcgctgatct ttgctatcct cggcgagctc cagcccgcag
     6361 cgcagggcca ggtacgagcg ccccatgccc ttgaagatgg ccatgacgcc gtcgccggta
     6421 tatttgatga ccgagccttc gtattgctgg atcttcttcg cctgcgcgct gagcagtcgg
     6481 ttgagcgtct cgaagaaatc gcgtggcggc atctcggtga tgagctggga ggagccgcgg
     6541 atatcggtga acaacaccgc cgcctcgaac agctcggctt cgctgtcgaa ttcgttatcg
     6601 agcgccgtca gcgcgccttc cggcaccagt tgtgcgagtt tgtccgcccg gttctccagt
     6661 tcacgcgtga ccagcttgac cttgagcagg tcttccaccg ccatatagaa gcgttcgtcc
     6721 gagagcggtt tctcgatgat gatgtcgatg cattccgcct gtttagccag ctcgcgcaca
     6781 gccttcagtg ccggaccggg cgaaacgatg gcgatgatgg cgctgtgcgg gctgatcagc
     6841 ttgatggtgc tacaggcgat caggctgtcc ggatattgcg agtgatagtt gaagatcaca
     6901 acatccggct tggtcgtctg caagacgcgc ggcaatgcca gtgcgtgcgc gacatgctgc
     6961 acatcggtga tattgcgccg ctggagatca tcgaggatga ccgaggaggt atagctgtcg
     7021 ctatccacga gtaataacct tggggtttgc atcatcctgt ctttcattgg tcgctatcac
     7081 gaggtaggtg gcgccagatt gcgccaccgt cctacgaccc tggcaagggc aaaaagattc
     7141 catgcggccg tatcgatcgt ggtcatcact agcgccagct gaccaagtca tctcgacagg
     7201 cgcacacggc ggctattggc cttacgccga gttttactct gggcctctga catagcgcag
     7261 acttaaaaaa atcctacaga cgcttcagcg ttagccctac atcgtcgcag tatgtcagtc
     7321 tttaggatgt gcacatgg
//
LOCUS       DATMTW010000338         6561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_398369, whole genome shotgun sequence.
ACCESSION   DATMTW010000338 DATMTW010000000
VERSION     DATMTW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398369"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..854)
                     /locus_tag="VN084_06640"
     CDS             complement(228..854)
                     /locus_tag="VN084_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HWT29173.1"
                     /translation="MSRRSAATLLACLLAACATTSGNTYYSLSRAGENGVQSDGAGKE
                     SIAHADDDRPAVELAPIRLPVAVDRSQIVLRRDDRVQVLETSRWSQPLKYELGSALAD
                     DLSRILPGYQVYSVAHALRRQPDIRIGLDIQRFDSERGVAATVEARWTIRDLRTQQTP
                     ISGHALVREPVADGSLDALAGAHARALREISKAISKSISEQIDQTIHP"
     gene            complement(851..2494)
                     /locus_tag="VN084_06645"
     CDS             complement(851..2494)
                     /locus_tag="VN084_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWT29174.1"
                     /translation="MSEDNPIPEAVLINRRRRLPQLVWLIPAIAVLISGWLLINTLAQ
                     RGPTVTITFLTAEGIEAGKTRIRHKDVDIGEVQSVKLSDDRSHVVVTAQLAKEAESFI
                     AADTRFWVVRPRVTGGKVLGIGTLLSGAYIGVDVGKSKETSTEFVGLETPPVLTGGMP
                     GSHFVLHAKDVGSLDIGSPVYFRHIQVGSVVAYELDKDGARAIIKIFINAPYDKFVAS
                     NSRFWNDSGVNLSMDANGIKLQSQSVVSVLLGGIAFESPDFGPGTTKAKENSHFDLHA
                     SYAEALKTPDSAPLVFHLQFNDSLRGLAPGAPVDFRGITVGEVLSVGVEYEQKREWFY
                     FPVTIALYPERIRLHGSALQDATNTADHQETIKQGLRQAINHRGFRAQLRNSSLLTGQ
                     LYIALDFFPDAEKKTVSWRKDPMEIPTVRGNLEELQATLNRVLAKLEKIPVDQISGDL
                     RKALKSMDQTAQSIDKLAKRIDGETVPEVSAGLQDLRSTLNTLDKLLASDSPLQQDTR
                     DAMREVGRAAESLRTLADTIERNPDALIWGLKYKKENKP"
     gene            complement(2469..3113)
                     /locus_tag="VN084_06650"
     CDS             complement(2469..3113)
                     /locus_tag="VN084_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWT29175.1"
                     /translation="MPAADPSTYRLTACHTCSQVSRLPGNNGVYACSRCGQALHARKP
                     YSVERAWALLITALVLFVPANLLPVMETGSLFGSQKDTIMSGVIYLWTSGSWPLAVVV
                     FLASIVIPLAKIVILAMLLFSVQFGAHWWPYERTRLYRLVELIGRWSMLDIYVVTMLV
                     ALVQMQTLAVVHAGPGALAFGAVVVLTMLAAMAFDPRLIWDNLKEDDVRRQPHP"
     gene            complement(3100..3705)
                     /locus_tag="VN084_06655"
     CDS             complement(3100..3705)
                     /locus_tag="VN084_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWT29176.1"
                     /translation="MRSAGLIACHDCDLLQREIALPPGAAAACGRCGAVLYRRNRTSQ
                     EVTLALTITALIVFVIANLFPIVTIAAQGQFHSTTLWGAVVALWQDDMRLVASLVLFT
                     TIVMPLLELVITTYLLAVPRPTPLALRLLTAISPWAMVEVFMLGLLVSVQKLSHLATI
                     IPGIGIWAFALLMLLFAAINSRFSISSLWQQTNHEVAHASR"
     gene            complement(3878..4627)
                     /locus_tag="VN084_06660"
     CDS             complement(3878..4627)
                     /locus_tag="VN084_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HWT29177.1"
                     /translation="MDTPPTTFAPGIYDIGITAYLNEHEHVSSSMLKRVLESPEALQR
                     YLARRHESAPVLDFGTAVHCALLEPERFEAEYLALPVERADLFHAADLALIEAERREV
                     HFITEAQMQAVRTIQNRVRQMPEVMALLEEGLAERSLFWQDEATGIRCKIRPDLLVLP
                     HLILELKTTFNPALAVFQRTALMQRYHLSAAMYLDGVRQVTGHSPNYMYLVAGRHAPH
                     EVLTFVPGEAMLREGERLYRRALLAIQDLPI"
     gene            complement(4633..4980)
                     /locus_tag="VN084_06665"
     CDS             complement(4633..4980)
                     /locus_tag="VN084_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWT29178.1"
                     /translation="MTGRIRRKLIADKPAQPTETAALVCPLCERVIPASQRDAHHLIP
                     KSKGGRQTEYLHRICHRQIHALFTETELARQYNHVEALLAHPKMARFVAWVKTKPEHF
                     YERTQKSLRLKSK"
     gene            complement(5024..5458)
                     /locus_tag="VN084_06670"
     CDS             complement(5024..5458)
                     /locus_tag="VN084_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWT29179.1"
                     /translation="MDIAEVAKLSGLPASTLRFYEEKGLIESRRSQGLRRRYADDILQ
                     RLALISLGRSAGFSLDEIVAMFTPAGPEIDRSLLMAKADTLDRRIAELTAMRDGLRHA
                     AACKAPDHLACPKFLRLLKLAANPRFRKAAELTPKKLKPRTS"
     gene            5582..6226
                     /gene="tmpT"
                     /locus_tag="VN084_06675"
     CDS             5582..6226
                     /gene="tmpT"
                     /locus_tag="VN084_06675"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HWT29180.1"
                     /translation="MEANFWHQKWQRGDIGFHLSQANPLLVAHLDSLKLKAGDRVFLP
                     LCGKTLDIGYLLHLGYRVTGAELSEIAIEALFQSLQLQPTVTEHGRLKHYRAETLEVY
                     VGDIFELKATQLGPIDAIYDRAALVALPAEVRQRYAAHLVEITHAAPQLVITYEYDQS
                     QIDGPPFSVDANEVERLYGKSYALNARETRAVEGGMKGKVAATETVWKFEKLKP"
     gene            complement(6271..>6561)
                     /locus_tag="VN084_06680"
     CDS             complement(6271..>6561)
                     /locus_tag="VN084_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29181.1"
                     /translation="KQWVFLAATAVFLTLSTYPAFSAESNETDLNKQRVTPPGSTRAS
                     SPAKDDVIYHPRTGTFKTGEELQEDRDRKLNPDTYDKTPKPTDVPPPMPKTQ"
BASE COUNT         1269 a         1940 c         2034 g         1318 t
ORIGIN      
        1 gctggctcac gcactacctg ggcgcgctgc agcatgacag cttgcgcatc ctgttcacca
       61 cgctgatcgt ggttgtgctt atgatctatg tggtgatgcc gcgctacacc cggctggtga
      121 gatcctggct gatgcggtga ggagaattct ggcctcgtcg ctgacgattt tgcctggccc
      181 gcgactgttt ggtcggtcag aggacgaatc tgccaacggc agatccgtta cgggtggatg
      241 gtttgatcga tctgttcgct gatcgatttg ctgatcgctt tactgatctc gcgcaatgcc
      301 cgcgcatggg cgccagccaa ggcatcgagg ctgccgtcag ccacgggctc gcgcaccagc
      361 gcatgacctg agatgggggt ctgttgtgta cgtagatcgc gaatcgtcca gcgcgcctcg
      421 acggtcgcgg cgacgccgcg ctcggaatcg aagcgctgaa tatcgagacc gatgcggata
      481 tccggctggc gccgcaaggc atgggcaacg ctgtagacct ggtagccggg cagtattcgg
      541 gaaagatcat cggccagcgc gctgcccagc tcgtatttga gcggctgcga ccagcggctg
      601 gtctccagca cttgtacgcg atcgtcgcgg cgcagcacga tctggctgcg atcgaccgcc
      661 accggtagcc ggatcggtgc cagttcgacg gctggccgat cgtcgtcggc atgggctatc
      721 gattctttac ctgcaccatc cgactgcacg ccgttttcgc cagcacggct cagcgagtag
      781 taggtgttgc cgctagtggt ggcgcaagcc gcgagcaggc atgccagcaa ggttgccgca
      841 gagcgccggc tcacggttta ttctccttct tgtacttcag gccccagatc aaggcatcgg
      901 gattacgttc gatggtgtcg gccaaggtgc gcagtgattc agccgcgcgg ccgacctcgc
      961 gcatggcgtc gcgcgtgtcc tgctggagcg gcgaatccga agccaacagt ttgtcgagcg
     1021 tattgagcgt gctgcgcaga tcctgcagtc cggcgctcac ttccggcacg gtttcgccgt
     1081 cgatgcgttt ggcgagcttg tcgatgctct gcgcggtctg gtccatgctc ttcagggcct
     1141 tgcgcagatc gccgctgatc tggtcgaccg ggatcttctc aagcttggcc aacacgcggt
     1201 tgagcgtggc ctgcagttct tcaagattgc cgcgcacggt tgggatctcc atcggatcct
     1261 tacgccagct gacggtcttc ttttccgcat ccgggaagaa atcgagcgcg atatagagct
     1321 ggccggtgag caggctactg ttgcgcagct gcgcgcggaa accgcgatgg ttgatggcct
     1381 ggcgcagccc ttgtttgatc gtctcctggt gatcggcagt gttggtggca tcctgcaggg
     1441 cgctgccatg cagccggatg cgctcaggat agagtgcgat ggtgacgggg aaatagaacc
     1501 actcacgctt ttgctcgtat tccacgccga cggatagcac ctcgccgacc gtgatgccgc
     1561 ggaaatcgac cggtgcgccc ggagccagcc cgcgcagcga atcattgaac tggagatgga
     1621 agaccagtgg cgcgctgtca ggcgtcttga gcgcctcggc atagctggcg tgcaggtcaa
     1681 aatgactgtt ttccttggct ttggttgtcc ccgggccgaa gtccgggctc tcgaaggcga
     1741 tgccgccgag cagcaccgac accactgatt gcgactgcag cttgatgccg ttggcgtcca
     1801 tcgagagatt cacgccgctg tcgttccaga agcggctatt gctggcgacg aacttgtcgt
     1861 agggcgcgtt gatgaagatc ttgatgatgg cgcgtgcgcc gtccttgtcg agttcgtagg
     1921 cgactaccga acctacctgg atatgtcgga aatacactgg cgagccgata tcgagcgaac
     1981 cgacatcctt ggcatgcagc acgaaatggc tgccgggcat gccgcccgtg aggactggcg
     2041 gggtttccag gccgacgaat tcggtgctgg tctctttcga tttgccaaca tcgacgccaa
     2101 tgtaggcacc cgacaacaac gtgccgatac cgagcacctt gccccccgtg acgcgcggcc
     2161 gcaccaccca gaaacgggtg tcggcggcga tgaagctctc ggcttctttc gccagttgtg
     2221 ccgtcaccac cacatgcgag cggtcgtccg acagcttcac gctctgcact tcgccgatat
     2281 cgacatcctt gtggcggata cgcgtcttgc cggcttcgat gccttctgcc gtgagaaagg
     2341 tgatggtcac agtggggcca cgctgcgcca gtgtgttgat caatagccag ccgctgatca
     2401 gcaccgcgat cgccgggatc agccagacca gttgcggcaa gcgacggcgg cggttgatga
     2461 gcaccgcttc agggatgggg ttgtcttctg acatcgtctt ccttcaggtt atcccagatc
     2521 agtctcggat cgaaagccat cgccgccagc atcgtcagca cgacgacggc gccaaaagcc
     2581 agggcaccgg gtccggcatg gaccacggca agggtttgca tctgcaccag cgccaccagc
     2641 atggtgacca catagatatc gagcatcgac cagcggccga tgagctccac caggcgatag
     2701 aggcgtgtgc gttcataagg ccaccaatgc gcgccgaatt gcaccgagaa caatagcatg
     2761 gcgaggatga cgatcttcgc cagcgggatg acgatactgg caaggaacac caccaccgcc
     2821 agcggccatg agccggaagt ccagagataa atgacgccac tcatgatggt atctttctgc
     2881 gagccgaaca gcgagccggt ttccatcacc ggcagcaggt tggccggcac gaacagcacg
     2941 agcgccgtga tgagcaaggc ccaggcccgc tcgacgctat agggcttgcg cgcatgcagg
     3001 gcttgtccac aacggctgca agcatagacg ccattattgc cgggcaagcg cgagacttgg
     3061 ctgcaggtgt ggcatgcagt cagccggtag gtcgatggat cagcggctgg catgggccac
     3121 ctcatggttc gtctgttgcc acaggctgct gatgctgaag cggctattga tggcggcgaa
     3181 cagcagcatg agcaaggcaa acgcccatat gccgataccg gggatgatcg tcgccaggtg
     3241 cgagagcttt tgcaccgaga ccagcaggcc cagcatgaac acctcgacca tggcccacgg
     3301 gctgatcgcc gtcagcaacc gcagggcgag cggcgtcggt cgcggcactg ccagcagata
     3361 ggtcgtgatc accagttcca gcagcggcat gacgatggtg gtgaacaaga ccagcgaagc
     3421 caccagccgc atatcgtctt gccaaagcgc gacgacggcg ccccacagcg tcgtgctgtg
     3481 gaattgccct tgtgccgcga tggtgacgat gggaaacaga ttggcgatga cgaacacgat
     3541 cagcgcggtg atggtcagcg ctagcgtgac ttcctggctg gtgcggttgc ggcggtacag
     3601 taccgcgcca cagcggccac aggctgcggc agcacccggt ggcaaggcga tctcgcgttg
     3661 caacaggtcg caatcatggc aggcgatgag cccggcggat ctcatggggc gttcttcggc
     3721 atgggtctgg acatgctatt ggtacttttt atgcattgca acccgtgtct gccagtggct
     3781 gcgcggtgag cagcctgtcg acagccggca tcggaaagct ccgcattggc aagcccagca
     3841 ttggcaagcc cggagccatc agccggttca ccaaccctta gatcggcaaa tcctgtatcg
     3901 ccagcaaggc acgccggtac aggcgctcgc cctcgcgcag catggcctcg cccggcacga
     3961 aggtcagcac ttcatgcggc gcgtggcggc cggccaccag atacatatag ttagggctat
     4021 ggccggtgac ttgccgcacg ccatcgagat acatcgccgc gctcaagtga tagcgctgca
     4081 tcagcgccgt acgctggaac acggccagcg cggggttgaa agtggtcttc agttcgagga
     4141 tgagatgcgg cagcaccagc agatcgggcc ggatcttgca gcggatgccg gtggcttcgt
     4201 cctgccagaa caggctgcgc tcggcgaggc cctcttctag cagggccatg acttcaggca
     4261 tttgccgcac ccgattctgg atagtgcgca cggcctgcat ctgtgcctcg gtgatgaaat
     4321 gcacctcgcg gcgctccgct tcgatcagcg cgaggtcggc cgcatggaac aggtccgcgc
     4381 gctccaccgg caaggccaga tactcggctt cgaaacgctc gggctccagc aaggcgcaat
     4441 gcacggcagt accaaaatcc agcacgggtg cagattcatg ccgacgggcg agataacgtt
     4501 gcaaagcctc gggcgattcg agcacgcgct tcagcatcga gcttgaaaca tgctcgtgct
     4561 cgttgaggta ggccgtaatg ccgatatcgt agatgcccgg cgcaaatgtg gtgggtggcg
     4621 tatccatgcc cgctattttg acttgagccg caggcttttt tgcgtgcgct cgtaaaaatg
     4681 ctccggcttg gttttgaccc aggcgacaaa gcgcgccatc ttggggtgcg ccagcaaggc
     4741 ctcgacatgg ttatattgcc gcgccagctc ggtttcggtg aacagtgcgt ggatctgccg
     4801 atgacagatg cggtgcaggt attcggtctg cctgccgccc ttggatttgg ggatcaagtg
     4861 gtgcgcatcc ctctgcgacg cagggatcac gcgctcgcac aacgggcaca ccagcgcggc
     4921 cgtttctgtt ggctgcgcgg gcttatcggc gatcagtttg cggcggatgc ggccagtcat
     4981 cgcgggctga tccctccgct ttgctccaat gcaacggcca atctcatgac gtgcgtggtt
     5041 tcagtttttt cggcgtaagt tctgcggctt tcctgaatcg tggattggca gccagcttga
     5101 gcaggcgcaa gaatttcggg caagccagat ggtccggcgc cttgcatgcc gccgcgtggc
     5161 gcaggccatc gcgcatcgcc gtcagttccg cgatcctacg gtccagcgta tcggccttgg
     5221 ccattagcag cgaacggtcg atctccgggc ccgccggcgt gaacatggcg acgatctcat
     5281 cgagcgagaa ccccgcgctg cggcccagcg agatcaaggc cagcctctgc aagatatcat
     5341 ccgcatagcg ccgccgcagg ccctggctac ggcgtgattc gatcaggcct ttttcctcgt
     5401 agaatcgcaa tgtggaagcc ggcaacccgg agagttttgc aacctcggcg atatccatcg
     5461 cagccctttc gcataagacg cttgacttaa agtcgacttg aacttgtatt ctgtcccatg
     5521 tcgaacgctg caacaactgc agcggcatta ttcaacgatc aatcggaatc agggagtgaa
     5581 catggaagcg aatttctggc accaaaaatg gcaacgtggc gatatcgggt ttcacctcag
     5641 ccaggcaaac cccttgctcg tcgcgcatct cgatagcctc aagctcaagg cgggcgatcg
     5701 cgtgtttctg cccctgtgcg gcaaaacgct cgatatcggc tatctgctac accttggcta
     5761 ccgcgtcacc ggcgcggagc tgagcgagat cgccatcgaa gccttgtttc agtcactgca
     5821 gctgcaaccc accgtcaccg agcatggccg cctcaagcac tatcgtgcag agacgttgga
     5881 agtctatgtc ggcgatatct tcgaactcaa ggcaacgcaa ctagggccca tcgacgccat
     5941 ttacgaccgc gccgccctcg ttgcactgcc cgctgaggtt cgtcagcgct atgccgcaca
     6001 cttggtggag atcacgcacg ccgccccgca gctcgtcatc acctatgaat acgaccaatc
     6061 gcaaatcgac ggcccgccct tttcggtgga tgccaacgaa gtcgaacggc tatacggaaa
     6121 atcgtatgcg ctcaacgcca gggaaacgcg ggcggttgaa ggtggcatga aaggcaaagt
     6181 cgctgcgacg gaaacagtgt ggaagtttga aaagctgaaa ccctgaacta ctccaaatca
     6241 atcgtacgat cttggcgttt caggattgtg ttattgcgtc ttcggcatcg gaggcggcac
     6301 atcggtgggc ttcggcgtct tgtcgtaagt gtccggattc aatttacggt cgcgatcttc
     6361 ctgcaattct tcacctgtct tgaatgtgcc ggtacgcggg tggtagatca catcgtcttt
     6421 agcgggcgat gatgcgcgtg tcgaacccgg cggcgtcaca cgctgcttgt tgaggtcggt
     6481 ttcgttggac tcggcactga aagcggggta agtggatagg gtcagaaaaa ccgcagttgc
     6541 ggcgagaaaa acccattgtt t
//
LOCUS       DATMTW010000339         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_64230, whole genome shotgun sequence.
ACCESSION   DATMTW010000339 DATMTW010000000
VERSION     DATMTW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64230"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VN084_06685"
     CDS             complement(<1..609)
                     /locus_tag="VN084_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HWT29182.1"
                     /translation="MAKRHVSLLCWLLPLAMYTGDAHAWGLVTHLYFAHSLLWAMPLL
                     DPRLQRAIKRFPELVMAGACLPDLAIVSPSFRTTHDWEYARRMLQAAKTDEETAIAVG
                     YASHLYVDVIAHNHFVPAHEAMWLDNDTMLTHIASEWAMDAHLSPLLDTSPGRLLRSH
                     QALLVAFISPLFRCSRRRTLKAVRALAFWDQVLRTVKLPHIIY"
     gene            737..1105
                     /locus_tag="VN084_06690"
     CDS             737..1105
                     /locus_tag="VN084_06690"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWT29183.1"
                     /translation="MESPYKGKTGLRRLINAFGYSVEGLKAAYKNEDAFRQEVRLAIV
                     LIPLAIYLGKSGIEDALMIGSVLLVVIVELLNSSIEATVDRISLENHMLAKRAKDIGS
                     AAVLISLVNLAVVWGCLIFG"
     gene            1249..2451
                     /locus_tag="VN084_06695"
     CDS             1249..2451
                     /locus_tag="VN084_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT29184.1"
                     /translation="MKILFISDVYFPRVNGVSTSIRTFVGEMRAMGHEVHLIAPDYGI
                     YTEDEHWIKRVPARSIYFDPEDKLMKYGAAIDRLIELRRESYDIIHIHTPFVAHYLGL
                     KLAQLLDVPCVETYHTFFEDYLHHYLPVIPTPMAKGLARMISRRQCNSVAAIVAPSKP
                     MLDVLRQYGVKTPGEVIPTGLQQHSFERADGAAFRAKYGIAHDRPVILFVGRVAHEKN
                     IGFLLRMTAELRKQQPNVLLVVAGEGPAEKSLHAMAAELNLQENIRFIGYLDRNTELN
                     ACYRAADVFVFSSKTETQGLVLLEAMAQAVPVVALAELGTKSILIEGQGALIAQEDEA
                     QFAQRVFTLLADEKKRKDLGEEARSYAMKRWSSRTQAERMLQFYQAVLSEDEDIQFID
                     RRKVARAA"
BASE COUNT          538 a          729 c          754 g          485 t
ORIGIN      
        1 gtagatgata tgcggcagct tcaccgtgcg cagtacctga tcccagaagg cgagcgcgcg
       61 cacagccttg agcgtacgcc gtctcgagca gcggaacaac ggcgagatga atgcgaccag
      121 caacgcctga tggctacgca acaagcgccc gggtgaggta tccagcagcg gcgaaaggtg
      181 ggcatccatc gcccactccg aggcgatatg cgtgagcatg gtgtcgttat ccagccacat
      241 ggcttcatgc gcaggcacga agtggttgtg ggcgataaca tcgacataca gatggctcgc
      301 gtagccaacg gcgatcgcag tctcctcgtc ggtcttggcg gcttgcaaca tgcgccgggc
      361 atattcccaa tcgtgcgtgg tgcggaacga tggcgagacg atggcgagat cgggcaggca
      421 ggcaccggcc atgaccagct cggggaaacg cttgatggcg cgctgtagcc gcgggtcgag
      481 caagggcatg gcccaaagca gggaatgcgc gaaatagagg tgggtgacca atccccaggc
      541 atgggcgtcg cccgtgtaca tcgccagcgg cagcaaccag cacagcaggg agacatgtcg
      601 ttttgccatg ctgcgcaatt tgccgccaca gcgtgtagcg attgtgacaa gatcatgatt
      661 ttattgtgac aagccatctg gcccagccag ctttgctata ctgcgcggct tgtcaccgac
      721 tcatgcaggg gaacgtatgg aaagcccata caaaggtaag actggtttac gacgcttgat
      781 caacgccttc ggttactcgg tcgaaggcct gaaagccgcc tacaagaacg aggacgcctt
      841 ccgccaggaa gtcaggctcg ccatcgtgtt gatcccgctg gccatctatc ttggcaagag
      901 cggcatcgag gacgccctga tgatcggcag cgtcctgctc gtcgtcatcg tcgagctgct
      961 caattcgtcg atcgaagcca cggtcgatcg catctcgctc gagaatcaca tgctggcgaa
     1021 acgcgccaag gatatcggta gcgccgccgt gctgatcagc ctcgtcaacc tggccgtagt
     1081 ctggggttgt ctgatctttg gataaggatt gagcgatcag gcatgcgcat tgtgactgat
     1141 cggtgacaag cgcaagtcat tcatccgatt gtcataatcg ctcgctaata tgccctcagc
     1201 tatggggctg ctgcttcgcg gcccctctaa aaccaaagag ggcagctagt gaagatacta
     1261 ttcatttccg atgtttactt cccccgcgtc aacggcgttt ccacctccat tcgcaccttt
     1321 gtcggtgaga tgcgggcgat gggccacgag gtccacctga tcgcgccgga ttacggcatc
     1381 tataccgagg acgagcactg gatcaagcgc gtgcccgctc gcagcatcta tttcgatccg
     1441 gaagacaagc tcatgaagta cggtgcggcg atcgaccggc tgatcgaact gcggcgcgaa
     1501 agctacgaca tcatccatat ccacacgccg ttcgtcgcgc actacctcgg gctcaagctc
     1561 gcgcagttgc tcgatgttcc ctgtgtcgag acctaccaca cctttttcga ggattatttg
     1621 caccactacc tgccggtgat ccccacgccg atggccaagg gcctggcccg catgatctct
     1681 cggcgccagt gcaacagcgt cgccgccatc gtcgcgcctt ccaagcccat gctcgatgtg
     1741 ttgcgccagt acggcgtgaa gacgccgggc gaggtcatcc cgactggctt gcagcagcac
     1801 agcttcgaac gtgccgacgg tgcggctttc cgcgccaagt acggcatcgc ccatgatcgg
     1861 ccggtgatcc tgttcgtcgg tcgcgtggca catgagaaga acatcggctt cctgctcagg
     1921 atgaccgctg aactgcgcaa acagcaaccg aacgtgttgc tggtggtcgc cggcgaaggc
     1981 ccggctgaaa agagcctgca tgccatggcg gccgagctga acctacagga gaacatccgt
     2041 ttcatcggct atctcgaccg taatactgaa ctcaatgcct gctatcgcgc ggcggacgtc
     2101 ttcgtgttct cgtccaagac cgagacccag ggcctggtgc tgctcgaagc catggcacaa
     2161 gccgtgcctg tggtcgcgct ggcggagctg ggcaccaaat ccattctcat cgaagggcag
     2221 ggcgcgctga tcgcgcagga agacgaagcc cagttcgcgc agcgcgtatt cacgctgctg
     2281 gcggatgaga agaagcgcaa ggatctgggt gaagaagcac gcagctatgc gatgaaacgc
     2341 tggtcgtcac gtacgcaggc ggagcgcatg ctgcagttct accaagcggt gttgagcgag
     2401 gatgaggata tccagttcat tgatcggcgg aaggtggcga gagcagccta actgtcattc
     2461 ccgcgaaggc gggaatccag gcaaaaataa ctatcgaaat ctggat
//
LOCUS       DATMTW010000340         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_264194, whole genome shotgun sequence.
ACCESSION   DATMTW010000340 DATMTW010000000
VERSION     DATMTW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264194"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2098
                     /locus_tag="VN084_06700"
     CDS             <1..2098
                     /locus_tag="VN084_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWT29185.1"
                     /translation="TTPWTLPANQAVSVNPGVFYYVVETIDEYFVLAEDLFEAALKRY
                     NKEAIGAGVKHCLGTALEGLLLQHPFDNRQVPVITGEHVTTDAGTGLVHTAAAHGNDD
                     WLVMRANYPEEKPRILIGGDGRFFTSELVELEAIRGLNRQEANKVILGVMQENGTLLA
                     SARLQHSFPHCWRHKTPLMQLATHQWFIGMNSTDAQGTSLREHANKAVDDTRFFPSWG
                     RARLEAMIKNRPDWCVSRQRNWGVPMPLFVHKETGEPHPKTLELLETVAKQVEQQGIE
                     AWFSLDGAAFLAQHAPGDADQYKKVTDTLDVWFDSGTTHAAVLKRRADLAFPADLYLE
                     GSDQHRGWFQSSLLTGCAIDGRAPYEALLTHGFVVDGAGHKMSKSKGNVVAPQKVMDT
                     YGADILRLWVASTDYSGELTISDEILKRVAEGYRRIRNTLRFLLANLADFDAGKDLLP
                     VEQWLEIDRYALKLTQDLQDSVRADYDRYEFHLAVQKLVSFCSEDLGGFYLDILKDRL
                     YTTGETSHPRRAAQSALHHITHTFMRLIAPILSFTADEIWQTLGMDADKTVFEDVWYA
                     LPEHGLSNERVADWQTLLEVRAMSNKAIEEKRGEGLVGSSLQSELDIYAQDATYRVLQ
                     SFGDDLRFLLIASRATVHEEAGSPLRIDVSASAHAKCDRCWHYRADVGADPAHPHICG
                     RCVSNLFGRGEPRRFA"
     gene            2108..>2304
                     /locus_tag="VN084_06705"
     CDS             2108..>2304
                     /locus_tag="VN084_06705"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWT29186.1"
                     /translation="MTLNLSRWLGLSAVVLALDLYTKQLVLKAFAYGEHVTVTSFFDL
                     VRYHNEGAAFSFLAAAGGWQR"
BASE COUNT          455 a          710 c          693 g          446 t
ORIGIN      
        1 cacaaccccc tggacattac ccgcaaatca ggcagtgtca gtaaatcctg gtgtttttta
       61 ttacgttgta gaaaccattg atgagtattt tgtattggcc gaggatttat ttgaggctgc
      121 actaaaaaga tataacaaag aagcaatagg cgcaggtgtt aagcattgtt taggaactgc
      181 tttagagggc ttgctactac agcatccatt cgacaaccgc caggtgccag tcatcaccgg
      241 cgagcacgtc accaccgacg ccggtaccgg gctggtgcat accgccgccg cccacggtaa
      301 cgacgactgg ctggtgatgc gcgccaacta tccggaagag aagccgcgta tcctgatcgg
      361 cggcgacggc cggttcttca ccagcgagct ggtcgaactg gaagcgatcc gcggcctaaa
      421 ccgccaggaa gcgaacaagg tcatcctggg ggtcatgcag gagaacggca cgctgctcgc
      481 cagcgcccgc ctgcagcata gcttcccgca ttgctggcgc cacaagacgc cgttgatgca
      541 gctggcgacc caccagtggt tcatcggcat gaattcgacc gacgcgcagg gaacaagctt
      601 gcgcgaacac gccaataagg ctgtcgacga cacgcgattc ttcccgagtt gggggcgtgc
      661 gcggctagaa gccatgatca agaaccgccc ggactggtgt gtctcgcgtc agcgcaactg
      721 gggcgtgccc atgccgctgt tcgtgcacaa ggagaccggc gagccgcatc caaagacact
      781 ggagctgctg gagaccgtcg ccaagcaggt cgagcagcaa ggcatcgaag cctggttctc
      841 gctcgacggc gccgcgttcc ttgcgcaaca tgcgcctggc gatgccgatc agtacaagaa
      901 ggtcaccgat accctggatg tctggttcga ttccggcacc acgcatgccg ccgtgctcaa
      961 gcgccgtgcg gatctcgcgt tcccggcgga cctttatctt gagggctcgg accagcatcg
     1021 cggctggttc cagagttcgt tgttgacggg ctgcgccatc gacggccgcg cgccctatga
     1081 ggcactgctc acccacggct tcgtggtcga cggcgccggt cacaagatga gcaaatccaa
     1141 gggcaacgtg gtcgcgccgc aaaaggtcat ggatacctat ggcgccgaca tcctgcgctt
     1201 gtgggtggcc tccaccgatt attccggcga gctgaccatc tccgacgaga tcctcaagcg
     1261 tgtggctgag ggctaccgcc gcatccgcaa caccttgcgc ttcctgctcg ccaacctggc
     1321 cgatttcgat gccggcaagg atctgctgcc ggtcgagcaa tggctggaga tcgaccgcta
     1381 cgcgctcaag ctgacgcagg atctgcagga tagcgtgcgc gccgattacg atcgctacga
     1441 gttccacctc gcggtgcaga agctggtcag tttctgctcg gaggacctcg gcggtttcta
     1501 tctcgatatc ctgaaagacc ggctctacac gacgggcgag acttcgcatc cgcgccgcgc
     1561 cgcgcagagc gcgctgcatc acatcacgca cacgttcatg cgcctgatcg cgcctatcct
     1621 cagcttcacc gctgacgaga tctggcagac gctgggcatg gacgccgaca agaccgtatt
     1681 cgaggatgtc tggtatgcgc tacccgagca tggcttgagc aacgagcgtg tcgcggactg
     1741 gcagaccttg ctggaggtac gcgccatgag caacaaggcc atcgaagaga agcgcggcga
     1801 aggcctggtc ggctcctcgc tccaatccga gctggatatc tatgcgcagg atgcgacgta
     1861 tcgtgtgctg caaagcttcg gcgacgatct gcgtttcctg ctcatcgcct cgcgcgccac
     1921 ggtgcatgaa gaagctgggt ctcctttgag aattgatgtc tcggcgagtg cgcatgccaa
     1981 atgcgatcgc tgctggcatt atcgcgccga cgtcggcgcc gatccggcgc atccgcacat
     2041 ctgcggtcgc tgcgtcagca acctgttcgg tcgcggcgaa ccgcgtcgct ttgcctgagc
     2101 gaatcccatg acactcaatc tgtcgagatg gctgggcttg agcgccgtgg tgttggcgct
     2161 cgacctctat accaagcaac tcgtgctcaa agccttcgct tacggcgagc acgtcacggt
     2221 caccagtttc ttcgaccttg tgcgttacca caacgagggc gcggcgttca gcttcctggc
     2281 tgcggccggg ggctggcagc gcat
//
LOCUS       DATMTW010000341         5508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_264199, whole genome shotgun sequence.
ACCESSION   DATMTW010000341 DATMTW010000000
VERSION     DATMTW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264199"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VN084_06710"
     CDS             <1..809
                     /locus_tag="VN084_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWT29187.1"
                     /translation="WRWWVVHLWVEGIFEVFATAIISVLFVLMGLLRGSVATTIVLFS
                     TIIFLGGGVLGTFHHLYWSGTPISVMALGATISALEVVPLMVVGFEAYSHAKLEKQAA
                     WQATYHWPFMFFSAVLVWNLIGAGLFGFLINPPIALYYMQGLNTTANHGHAALFGVYG
                     MLGLGLMLFCLRGLTDVTRWNRKLLSLSFWSLNIGLAIMTFLSLLPQGLWQTYASIKH
                     SYAYARSAEFMHSRLMEGLVWARVPGDVVFAIGAIAFALFVWQAFRSNPR"
     gene            806..1531
                     /locus_tag="VN084_06715"
     CDS             806..1531
                     /locus_tag="VN084_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29188.1"
                     /translation="MKSKRLPSYEWDAIAGILAAVVALVLHMLHVVDESIVLPILLAL
                     LGLLFLNFMRHTHSNERTAEQVDMIANELTQMQQWLKAPEVLLVGPRQLRSVNEQFVR
                     QMRGDTLWYNLCLSMYAPAPLFDALLRPALENPLVTSVQFVLDTSQRALWQDVIQPRI
                     ASCSGNGKVREPRWCKLDKTLSFIMADSQASGGTEALLSFWGEPFMAQSNARDIPRFI
                     FHVQKDSTLLPHLVEVQRNALTA"
     gene            complement(1564..2319)
                     /gene="rlmB"
                     /locus_tag="VN084_06720"
     CDS             complement(1564..2319)
                     /gene="rlmB"
                     /locus_tag="VN084_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWT29189.1"
                     /translation="MSDTRILFGFHAVLSRLRQHAASIQEILIDRDRVDARMKDLLGM
                     AEAAGVRLMQVERSRLDGMAGANGRHQGVIARVVDTPIPYRDIHDILESDLKEPPFFL
                     VLDGVEDPHNLGACLRVADAMGVHAVIAPKDRAAGLNATVRKVACGAAETVPFIAVTN
                     LARTLRELKEAGVFVVGASAEANHDLFSTKLTGPLALVLGAEGTGLRRLTAETCDALV
                     GIPMFGSVESLNVSVASGICLYEARRQRNSTTG"
     gene            complement(2316..4700)
                     /gene="rnr"
                     /locus_tag="VN084_06725"
     CDS             complement(2316..4700)
                     /gene="rnr"
                     /locus_tag="VN084_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HWT29190.1"
                     /translation="MSTRKKTSDHKKHSQRPADPHAAREAANYEHPLPSRELIMQQMD
                     EQGVPLSVEKLYELLGIHEEEREIFNRRLNAMEREGQIIRNRKGALCLAQKIHLLAGK
                     VQGHPDGFGFFIPDSGGEDLFLSPKEMSQVLHGDRVMVRQSGFDRRGRPEGKIVEVLE
                     RTNKNLVGRVMRGFGFTIVAAEDKRVNQDIIIPYHLDMDAKDGQVVMVELTEQPSPHG
                     KPIGKIVEILGNYADSGMEIEIALRKHKLPYEFSPQAEKQAEAYPKLVQAKDWKGRID
                     LRELPLITIDGETARDFDDAVFCEPQGQGWRLVVAIADVSFYVRPGDALDKDAYERGN
                     SVYFPRRVIPMLPEALSNGLCSLNPDVERMCMVCDMQIDGQGNVKRYQFYPSVMRSKA
                     RMTYTKVYDMLTNPRGEAAREHAWLMPHVQHLYTLFKVMLSAREKRGAIEFESSETVM
                     VFNDNGKIDRIEASTRNDAHRLIEECMLAANVCASDFLKAHEHPALYRIHEGPTPEKL
                     EALRTFMGEFGFGIGGGDTPHAKDYAKVLARIKGRPDELLLQTVLLRSMQQAVYSPDN
                     VGHFGLAYESYTHFTSPIRRYPDLLIHRAIKAVIEGRKYTPGDWQALGQHCSMTERRA
                     DDATRDVQAWLKCYYMQDKIGEVFDGTVAGVTSFGLFIALDGVYVEGLLHVTELGNDY
                     FNYDKARHEMAGERTGVRYRLGDRLRVKVARVDLETSKIDFILVAATTKLGKDESAPA
                     AVTAAKDRPRPRQSADGGEKARSGKSRADGTRPAKSGKAPAAGKSTNRKGSKRK"
     gene            4751..4837
                     /locus_tag="VN084_06730"
     tRNA            4751..4837
                     /locus_tag="VN084_06730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4785..4787,aa:Leu,seq:cag)
     gene            5094..>5508
                     /locus_tag="VN084_06735"
     CDS             5094..>5508
                     /locus_tag="VN084_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWT29191.1"
                     /translation="MPLRLRHLFAATLLATSLSANATTDSYGTLLAGSFQPSDTFATL
                     TYSNVGNLYSFTLNALDLDALFTPGAFIGAIAVDAASQPVVSNVSGDTVVSVSPAGGP
                     TGTYDFRFDLTGPKQARLTANESVSFDALFSQAVSL"
BASE COUNT         1007 a         1701 c         1579 g         1221 t
ORIGIN      
        1 actggcgctg gtgggtcgtc catctgtggg tcgagggcat cttcgaggtc ttcgccaccg
       61 ccatcatctc ggtgctgttc gtgctcatgg gtttgctgcg ggggtctgtc gcgaccacga
      121 tagtgctgtt ctccaccatc atctttctcg gaggcggcgt actcggcacg ttccaccatt
      181 tgtactggag cggcaccccg atctcggtca tggcgctagg cgcgacgatc tcggcgctgg
      241 aggtggtgcc gctgatggtc gtcggcttcg aggcctacag ccatgcgaaa ctggaaaagc
      301 aggccgcatg gcaagcgacc tatcattggc cgttcatgtt cttcagcgcc gtgctggtct
      361 ggaacctgat cggcgctggc ctgttcggct ttctcatcaa cccgcccatc gcgctctact
      421 acatgcaggg cctcaatacc accgccaatc atggccatgc cgccctgttc ggcgtctatg
      481 gcatgctggg gcttggcctg atgctcttct gcctgcgcgg gctcaccgat gtgacgcgct
      541 ggaatcgcaa actgctgtcc ttgagcttct ggagcctgaa tatcggcctt gccataatga
      601 ccttcctctc cttgctgccg caagggctat ggcagaccta tgccagcatc aagcattcct
      661 acgcctatgc gcgttcggca gagttcatgc atagcagatt gatggaaggc ctggtctggg
      721 cacgcgtgcc cggcgacgtc gtgttcgcga tcggcgccat cgcgttcgcc ctctttgtct
      781 ggcaagcctt caggagtaac ccgcgatgaa gtcgaaacgc ctacccagct atgaatggga
      841 tgccatcgcc ggcatcctcg ccgccgtggt tgccctcgtg ctgcatatgc tgcatgtggt
      901 ggatgagtcc atcgtgctgc ccatcctgct cgcattgctg ggcctactgt tccttaactt
      961 catgcggcat acgcacagca acgagcgcac ggcagaacaa gtcgacatga tcgctaatga
     1021 gctgacgcag atgcaacagt ggctgaaagc gccggaagtc ctgctggtcg gcccgcgcca
     1081 gctacgatcc gtcaacgagc agttcgtgcg acagatgcgc ggcgacacgc tctggtacaa
     1141 cctgtgcctc agcatgtatg cgccagcgcc gctgttcgat gccctgcttc gcccggcact
     1201 ggagaatccg ctcgtgacct cggtgcaatt cgtgctcgac acgagccagc gagcgttatg
     1261 gcaagacgtg atacagccgc gtatcgcatc ctgttcgggg aacggcaagg tgcgggagcc
     1321 gcgctggtgc aagctggaca agacattgtc cttcatcatg gcggatagcc aggcgagcgg
     1381 cggtactgaa gcgctgctaa gtttctgggg tgaacctttc atggcacaga gcaacgcacg
     1441 tgatatcccg cgtttcatct tccatgtgca gaaggattcc acattgctgc cgcatctcgt
     1501 cgaggtgcag cgcaacgcct tgacggcctg agcctgcatg cgccgagatt cggcgccatc
     1561 aggtcagccg gtcgtcgaat tgcgctggcg gcgcgcttcg taaagacaga tgccgctagc
     1621 gaccgagacg ttgaggctct cgaccgagcc gaacatcggg ataccgacca aggcatcaca
     1681 ggtttcggcg gtcagtcggc gtaaacccgt gccttccgcg cccagcacca gcgccaatgg
     1741 cccggtcagc tttgtcgaaa acaagtcatg attcgcttca gccgatgcgc cgacgacgaa
     1801 cacgccggcc tctttcaatt cgcgcaaggt gcgcgcgaga ttggtgactg cgataaaagg
     1861 taccgtctcg gccgcgccgc aggcaacctt gcgcaccgtg gcgttcaaac cggccgcccg
     1921 atccttgggc gcaatcaccg catgcacgcc catggcgtcg gccacacgca agcaggcgcc
     1981 gagattgtgc gggtcttcca cgccatccag caccagaaag aacggcggtt ccttgagatc
     2041 ggattcgagg atgtcatgga tatcacggta cgggatcggc gtgtcgacca cccgggcgat
     2101 cacgccctga tgacgcccat tggcacccgc catgccgtcg agccgcgaac gctcgacctg
     2161 catcaggcgc acgcctgcgg cttctgccat gcccagcagg tctttcatgc gcgcgtcgac
     2221 gcgatcacga tcgatcagga tctcctggat actggcggca tgctggcgca aacggctaag
     2281 cactgcatgg aagccaaata ggatacgggt gtcgctcatt tacgtttact gccttttcta
     2341 ttcgttgatt tgccagctgc cggcgccttg ccggattttg ctggcctggt gccatccgcg
     2401 cgggatttac cggaccgagc cttctccccg ccgtcagccg actgccgcgg ccgcggcctg
     2461 tcttttgccg ctgttaccgc cgcaggcgcc gattcatcct tgccaagctt ggtcgtcgcc
     2521 gcaaccagta tgaaatcgat cttgctggtc tccaggtcga cgcgcgcgac tttcacgcgc
     2581 aagcgatcgc ccaggcgata acgcacgccg gtccgctcgc ccgccatctc gtggcgcgcc
     2641 ttgtcatagt tgaagtaatc attccccaac tcggtgacat gcagcaggcc ttcgacatag
     2701 acgccgtcga gcgcgatgaa gaggccgaag ctggtgacgc ctgccaccgt gccatcgaat
     2761 acttcgccga tcttgtcctg catgtaataa cacttgagcc atgcctggac gtcacgcgtc
     2821 gcatcgtcag cgcgccgctc ggtcatggag cagtgctggc ccagtgcttg ccagtcgccc
     2881 ggcgtgtatt tcctgccctc gatcactgcc ttgatcgccc gatggatcag aagatcgggg
     2941 taacggcgga tcggcgacgt gaaatgcgta tacgattcat aggccaggcc gaaatggccg
     3001 acattgtccg ggctgtagac cgcctgttgc atcgaccgca acaataccgt ctgcaacagc
     3061 agttcgtctg gacgtccctt gatgcgggcg agcaccttag cataatcctt ggcatgcggg
     3121 gtatcgccgc cgccgatgcc gaaaccgaat tcccccataa aggtgcgcaa cgcttcgagt
     3181 ttttctggcg tcgggccttc atggatgcgg tataacgcag ggtgttcgtg cgccttgaga
     3241 aaatccgaag cgcagacatt ggcggcgagc atgcattcct cgatcagccg gtgggcgtcg
     3301 ttgcgcgtgg aggcttcgat ccggtcgatc ttgccgttgt cgttgaagac catgacagtc
     3361 tcgctgcttt cgaactcgat cgcgccgcgt ttctcacgcg cgctcagcat caccttgaac
     3421 agggtataaa gatgctgcac atgcggcatc agccaggcgt gctcgcgagc ggcctcgccg
     3481 cgcgggttgg tcagcatgtc gtagaccttg gtgtaagtca tgcgggcctt ggagcgcatc
     3541 accgacggat agaactgata gcgcttgacg ttgccctggc catcgatctg catatcacag
     3601 accatgcaca tgcgctcgac atccggattc aacgagcaaa ggccgttgga cagggcttca
     3661 gggagcatgg gaatgacgcg gcgtgggaaa tataccgagt tgccgcgctc gtaagcgtcc
     3721 ttatcaagcg cgtcgccagg tctgacatag aagctgacat cggcgatcgc caccaccaga
     3781 cgccagcctt ggccctgagg ctcacaaaat acggcatcgt caaagtcgcg tgcggtttcg
     3841 ccgtcgatgg tgatgagcgg cagttcgcgc aggtcgatgc ggcctttcca gtccttggcc
     3901 tgcaccagct tggggtaggc ttcggcctgc ttctcggctt gcggcgagaa ctcgtacggc
     3961 agcttgtgct tgcgcaatgc gatctcgatc tccatgccgg aatctgcata gttgccgagg
     4021 atctcgacga tcttgccaat cggtttgcca tgaggcgacg gctgctcggt cagctcgacc
     4081 atgaccacct ggccgtcctt ggcgtccata tcgaggtggt aagggatgat gatgtcctgg
     4141 ttcacgcgct tgtcttcggc cgccacgata gtgaacccga agccgcgcat gacacggccg
     4201 accaggttct tgttggtacg ttccagcacc tcgacgatct tgccctctgg ccgaccgcga
     4261 cggtcgaacc cggactggcg caccatgaca cggtcgccgt gcaacacctg cgacatctcc
     4321 ttggggctca ggaacaggtc ctcgccgcca ctgtcgggaa tgaagaagcc aaagccgtcc
     4381 ggatggccct gcaccttgcc ggccagcaga tggatctttt gcgccagaca caaggcgccc
     4441 ttgcggttac ggatgatctg cccctcgcgc tccatggcgt taaggcggcg gttgaatatc
     4501 tcgcgctctt cttcgtggat gcctagcaat tcgtagagtt tttcgacact gagtggcacg
     4561 ccctgctcat ccatctgttg catgatgagc tcgcggctgg gtaatggatg ttcgtagttg
     4621 gcggcttcgc gagccgcatg aggatcggcg gggcgctggc tatgtttttt atggtcggat
     4681 gttttttttc tcgttgacaa agtatgtctt tcctatagaa ttcgcgttct tcgttttacc
     4741 ccatttgatt gcccagatgg cggaattggt agacgcgcta ggttcaggtc ctagtggtgg
     4801 caacactgtg gaggttcgag tcctcttctg ggcaccaaag ttcatgcgtt tctgaaccct
     4861 tacttggttt ccccaccaca agttgtttca taatttatga cgcagaccat gcattttaca
     4921 tgaatctgcc atacccgtct gacgaagccg cttgaatcca cgctgttctc cctgcttagc
     4981 gccttggccc gaggccacaa aaaactattt ctcgttgcgt gtccgtccga acatacccgt
     5041 gtcgtaccac atgctaaaag gggacttctt tcctcacgct catggagttc atcatgccct
     5101 tacgacttcg ccatcttttc gcagcgacgc tcctcgcaac cagtctatcc gcaaacgcta
     5161 ccaccgatag ctacggcacc ttgctcgccg gtagtttcca gccgtccgat acgtttgcca
     5221 cccttaccta ttccaatgtc ggcaaccttt acagcttcac cctgaacgcg ctcgacctgg
     5281 atgcgctatt cacgccgggt gcattcattg gcgccatcgc cgtcgatgcc gccagccagc
     5341 ccgtcgtttc gaatgtcagc ggcgataccg tcgtcagcgt cagtcccgct ggcggcccaa
     5401 ctggcaccta cgatttccgc ttcgacctga ccggtcccaa gcaggccaga ttgaccgcga
     5461 acgaatcggt gtcgttcgat gcgttgttca gccaggcggt ctctttga
//
LOCUS       DATMTW010000342         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_64497, whole genome shotgun sequence.
ACCESSION   DATMTW010000342 DATMTW010000000
VERSION     DATMTW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64497"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1875
                     /locus_tag="VN084_06740"
     CDS             <1..>1875
                     /locus_tag="VN084_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWT29192.1"
                     /translation="LQPGKMLLIDMEQGRIIDDAEVKQQLAEAKPYKQWIEKSRYFLG
                     DLPKVERKPELAASLLDVQQAFGYTQEDIKFILQPMIANSEEASGSMGNDSALPVLSN
                     KPKQLYNYFKQLFAQVTNPPIDPIREELVMSLVTFIGPKPNLLGIDETDPPLRLEASQ
                     PVLTLDELEQLKAIDTLTQNRYRALTLDITYPVAQGKQHMAAAIEALNKAAEKAVNDG
                     FNVLILSDRQVSAERLAIPALLACSATHQHLVKAGLRTSTGLVIDTGSAREVHHFALL
                     GGYGAEAVCPWLAFETISELAAAHGTDAKEAGKKFVKAIGKGLYKVMSKMGISTYQSY
                     CGAQIFEAVGLNSRFVKQYFVGTATNVEGIGLDEVAEESVRLHDAAFGDDPVLAAALD
                     AGGEYAFRIRGEEHMWTPDSIAKLQHATRSGQFDTYKEYAKLINDQTKRHMTLRGLFD
                     IKPAGSPVPLEEVEAAKEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGG
                     EDPKRFIPVASDTSMASVLGAEKIEKDIALKAGDSMRSKIKQVASGRFGVTAEYLANA
                     DQIQIKMAQGAKPGEGGQLPGHKVSPYIASLRFSVPGVGLISPPPHHDIYSIEDLAQL
                     I"
BASE COUNT          408 a          597 c          546 g          324 t
ORIGIN      
        1 ctgcagccgg gcaagatgct gctcatcgac atggaacagg gccgcatcat cgatgatgcc
       61 gaagtcaagc agcagttggc tgaagccaaa ccctacaagc aatggatcga gaagtcgcgt
      121 tacttcctcg gtgacctgcc caaggtcgaa cgcaagcctg aactggccgc cagcctgctc
      181 gacgtgcagc aggcctttgg ctatacccag gaggacataa agttcatcct gcagccgatg
      241 atcgccaaca gcgaagaggc ctccggctcc atgggtaacg acagtgcgct gcctgtgctc
      301 tccaacaagc ccaagcagct ctacaattat ttcaagcagc tgttcgcaca ggtgaccaac
      361 ccgccgatcg acccgatccg cgaagagctg gtcatgtcgc tggtgacctt catcggtccc
      421 aagcccaacc tgctcggcat cgacgagact gacccaccgt tgcgtctgga ggccagccag
      481 ccagtgctga ccctggacga gctggaacag ctcaaagcca tcgacaccct gacgcagaat
      541 cgctaccgcg ccctgacgct cgacatcacc tacccggtgg cgcaaggcaa gcagcacatg
      601 gcagcagcta tcgaagccct gaacaaggcc gcggaaaagg ccgtcaatga cggcttcaac
      661 gtgttgatcc tgtctgatcg ccaggtcagc gccgaacggc tggcgatccc tgcgctattg
      721 gcctgttccg cgacgcatca gcatctggtc aaggcgggtc tgcggaccag caccggtctc
      781 gtcatcgaca ccggttcggc acgcgaagtc caccatttcg cgttgcttgg cggttacggt
      841 gcagaagcgg tctgcccgtg gctcgccttc gaaaccatca gcgagctcgc ggcagcgcat
      901 ggcaccgatg cgaaggaagc cggcaagaaa ttcgtcaagg cgatcggcaa gggcctgtac
      961 aaggtcatgt ccaagatggg catctccact tatcagtcct actgcggtgc gcagatcttt
     1021 gaagcggtcg gccttaacag ccggttcgtg aaacagtatt tcgtcggcac agcaaccaat
     1081 gtcgaaggca tcggcctcga tgaggtggct gaagagagcg tgcgtctgca cgacgcagcc
     1141 tttggcgacg acccggtgct ggccgccgca ctcgatgccg gcggcgaata tgccttccgt
     1201 atccgcggtg aagagcacat gtggacgccg gattccatcg ccaagctgca gcatgccacg
     1261 cgctctggcc agttcgacac ttacaaggaa tacgccaagc tgatcaacga ccagaccaag
     1321 cgccacatga cgctgcgtgg gctgttcgat atcaagccgg ctggttcgcc tgtaccgctc
     1381 gaggaagtcg aagcggccaa ggagatcgtc aagcgcttcg ccacgggtgc gatgtcgcta
     1441 ggctcgatct cgaccgaggc gcacgcgacc ctggccatcg ccatgaaccg tatcggcggc
     1501 aagtcgaata ccggcgaggg tggcgaagat cccaagcgat tcatcccggt cgccagcgac
     1561 accagcatgg ctagcgtcct cggcgccgag aagatcgaaa aggatatcgc gctcaaggct
     1621 ggcgattcca tgcgctccaa gatcaagcaa gtggcttccg gtcgtttcgg cgtcaccgcc
     1681 gaatacctgg ccaacgccga ccagatccag atcaagatgg cgcaaggcgc caagcctggt
     1741 gaaggcggcc agctgccggg ccacaaggtg agcccctaca tcgccagcct gcgcttctcg
     1801 gtgcctggcg tcggcctcat ctcgccgccg ccgcaccatg atatctattc gatcgaagat
     1861 ctggcgcagc tgatc
//
LOCUS       DATMTW010000343         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_533538, whole genome shotgun sequence.
ACCESSION   DATMTW010000343 DATMTW010000000
VERSION     DATMTW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533538"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="mtgA"
                     /locus_tag="VN084_06745"
     CDS             complement(<1..679)
                     /gene="mtgA"
                     /locus_tag="VN084_06745"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWT29193.1"
                     /translation="MSLKGFLWRAVLTLSLLVVLYQLWIFIHICWWIDHNPSRSAFMN
                     SSLAEMQAIDPDATLKHQWVDYKRISPNLKRALIAAEDTKFIDHEGFDWEGIQKAYEK
                     NLKKGRIVAGGSTISQQLAKNLFLSTRRTPWRKLEEAAITFMLEQMMTKRRIFEIYLN
                     IIEWGNGIFGAEAAARHYYGTSAANLNAYQAARLAAMVPKPKFYQKHPSARGLARRTA
                     TIQARMHL"
     gene            complement(676..1503)
                     /gene="aroE"
                     /locus_tag="VN084_06750"
     CDS             complement(676..1503)
                     /gene="aroE"
                     /locus_tag="VN084_06750"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWT29194.1"
                     /translation="MTARYAVIGNPIEHSKSPLIHHAFARQTGQDIVYERVLAPLDAF
                     PQVLQAMVAQGYAGANVTVPFKLEAFRCAGANLTERAMDAGAVNTLVFTEQGIVGDNT
                     DGIGLVTDIQQNLKVPLAGKRVLLIGAGGAAEGVLHPLLEQGPSLLVIANRTLDKALR
                     MVKKIEEQGDFRYVSVNAYAFDDLQGQSFDIVINATSAGLSDSTLPLPPGIFAPGALA
                     YDMMYGRETPFMRFARTHGAARIADGLGMLVEQAAEAFALWRGVRPETAPVIAQLRT"
     gene            complement(1506..1961)
                     /locus_tag="VN084_06755"
     CDS             complement(1506..1961)
                     /locus_tag="VN084_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWT29195.1"
                     /translation="METLNQILQRARDRAMQHELPYAGALTPVEAAHILQLASNAVLV
                     DVRSRAELELVGRIPAAQHIEWAFYPGMVPNPDFATQLDAQVDKELLTMFICRTGGRS
                     HNAAAVAAKLGFTEAYNVLEGFEGEATPDTRQRSRINGWKAADLPWTNG"
BASE COUNT          398 a          593 c          601 g          419 t
ORIGIN      
        1 ccagatgcat gcgcgcctgg atggtcgcgg tacgccgtgc cagtccgcgc gctgacggat
       61 gtttctggta aaacttgggc ttcggcacca tggccgccag ccgcgctgcc tgatacgcgt
      121 tgagattggc ggcactggtg ccgtaataat ggcgtgcggc ggcttcggca ccgaagatgc
      181 cattgcccca ttcgatgata ttgagataga tctcgaagat gcgacgctta gtcatcatct
      241 gctccagcat gaaagtgatc gccgcttctt ccagcttgcg ccacggcgtg cgcctggtcg
      301 acaggaacag gttcttcgcc aattgctggc tgatggtaga gccgccagca acgatcctgc
      361 ccttcttcag gttcttttca taagctttct ggatcccttc ccaatcaaag ccctcgtggt
      421 cgatgaactt ggtgtcctcg gccgcgatca aggcgcgctt gagattgggt gagatacgtt
      481 tgtaatcgac ccattgatgc ttgagcgtgg catcggggtc aatggcttgc atctcggcca
      541 gcgacgaatt catgaatgcg ctacgagacg gattgtgatc gatccaccag cagatgtgga
      601 taaagatcca tagctgataa agcacgacca gcagcgagag cgtcaatacc gcacgccaga
      661 ggaagccctt caagctcatg tgcgtagctg ggcgatgacg ggcgcggtct ctggtcgtac
      721 accgcgccag agtgcaaagg cttctgcggc ctgttcgacc agcatgccaa ggccatcggc
      781 aatacgcgcg gcgccatgtg tgcgcgcaaa gcgcatgaag ggcgtctcgc gcccgtacat
      841 catgtcatag gccagcgccc cgggggcgaa gatgccgggc ggcaggggta aggtactatc
      901 gctgaggccg gccgaggtgg cattgatgac gatatcgaag gattggcctt gcaggtcatc
      961 gaaggcatag gcgttgaccg agacatagcg aaaatccccc tgctcctcga tcttcttcac
     1021 catgcgcaaa gccttgtcca acgtacgatt ggcgatcacg agcagactcg gcccctgttc
     1081 cagcaatgga tgcagcacgc cctccgctgc gccgccggca ccgatcagca gcacgcgctt
     1141 gccggccaat ggcaccttga ggttttgctg gatatcggtc accaggccga taccgtcggt
     1201 gttatcgcca acgatacctt gttcagtaaa aaccagtgta tttaccgcgc cagcatccat
     1261 tgcacgttcg gtcagatttg ctcccgcgca cctgaaagcc tccagcttga acggcaccgt
     1321 aacattggca cccgcgtaac cttgcgccac catcgcctgc aagacctgcg ggaaggcgtc
     1381 gagcggcgcc agtacgcgct cgtagacgat atcctgcccg gtctggcgag cgaacgcatg
     1441 gtggatcagg ggagacttgc tatgttcgat cggattgccg atgaccgcgt aacgcgccgt
     1501 catggtcaac cgttggtcca gggcaggtcg gccgctttcc agccgttgat ccggctccgc
     1561 tgccgggtat ccggcgtggc ttcgccttca aaaccttcaa gcacgttgta ggcctcggtg
     1621 aagccgagct tggccgctac cgcggctgcg ttgtgtgaac gcccccctgt gcgacagatg
     1681 aacatggtca gcaattcctt gtcgacctgg gcatcgagct gcgtggcgaa atccggattg
     1741 ggaaccatcc ccggatagaa ggcccattcg atatgctgcg cggcggggat acgtcctacc
     1801 agctccagct ctgcacggct gcggacatcg accaggaccg cgttggacgc gagctgcagg
     1861 atgtgagcgg cttccactgg cgtcaatgcg ccggcatagg gcaattcatg ctgcatcgca
     1921 cggtcgcgcg cgcgttgcag gatctgattg agggtttcca tggcatccac ctttcttgtg
     1981 cctgaacaag caggaagtat agcgttatta c
//
LOCUS       DATMTW010000344         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_30277, whole genome shotgun sequence.
ACCESSION   DATMTW010000344 DATMTW010000000
VERSION     DATMTW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30277"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1217)
                     /gene="ruvB"
                     /locus_tag="VN084_06760"
     CDS             complement(183..1217)
                     /gene="ruvB"
                     /locus_tag="VN084_06760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWT29196.1"
                     /translation="MIETDRLIAADAQSPQEEALERALRPKLLDEYIGQEKARGQLEI
                     FINAARGRSEALDHVLLFGPPGLGKTTLAHIIAKEMGVNLRQTSGPVLERAGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPAMEDYRLDIMIGEGPAARSVRLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVSRLEFYTSEELGRIVHRSAGLLEVEMQEAGALEIAKRSR
                     GTPRIANRLLRRVRDYAQVKADGIVSPEIADAALKMLDVDKLGFDVMDRKLLQAVLEK
                     FGGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGYLMRTPRGRVATQAAYQHFGLPMP
                     ATLERQLFDQ"
     gene            complement(1221..1802)
                     /gene="ruvA"
                     /locus_tag="VN084_06765"
     CDS             complement(1221..1802)
                     /gene="ruvA"
                     /locus_tag="VN084_06765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWT29197.1"
                     /translation="MIGRLSGILLEKTPPQVLIDINGVGYECEVPMSTFYNLPAIGEK
                     VVLLTQFIVREDAQLLYGFGSDAERMTFRQLLKVNGIGAKSALSILSGLSVDDLMQAV
                     ALQEPALLTRVPGIGKKTAERLLLELKDKFTIDGAVPGAAGHGKSASGDVLNALIALG
                     YNEREALAAVKQLPAEATVADGIKHALKTLSKT"
     gene            complement(1816..2367)
                     /gene="ruvC"
                     /locus_tag="VN084_06770"
     CDS             complement(1816..2367)
                     /gene="ruvC"
                     /locus_tag="VN084_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWT29198.1"
                     /translation="MQPLTTRILGIDPGLRLTGFGVIEKIGEKLAYVASGTIKTTTGK
                     GADIEDLPSRLKIILESLAEVIETYQPQQVAVEKVFVNVNPQSTLLLGQARGAAISAA
                     VIKHLPVAEYTALQVKQSVVGNGHAAKEQVQEMVKRLLNLPGVPKPDSADALACAICH
                     AHGGQGLGKLATAGFRVRGGRLV"
BASE COUNT          453 a          804 c          771 g          497 t
ORIGIN      
        1 tcggtatcct cgtaatagac ccgcaccggc cagaggaatt ccgtcgtctc ggctgccccg
       61 cccgtatgat cgggatgcgc gctcaatcgc agctcacccg gtgacgagcc ttgtgtcggc
      121 aagccgcgtg cagcggctcg caagtcaatc cgccacgatt cgggcgggct ggatatccgg
      181 cgctactgat caaacaattg cctttccaat gttgcaggca tcggcaatcc gaaatgctga
      241 tacgccgcct gggtagcgac acgcccgcgc ggcgtacgca tgaggtagcc ctgctggatc
      301 aggtaaggct ccagcacatc ttcgatggtg tcgcgttcct cgccgatggc cgcagcgaga
      361 ttgtcgagac ccacaggccc gccgccgaac ttctccagca cggcctgcag caactttcgg
      421 tccatgacgt cgaagcccag cttgtcgaca tccagcatct tcagggccgc gtcggcgatc
      481 tcaggcgaaa cgatgccgtc ggccttcacc tgggcatagt cacgtacgcg ccgcagcaag
      541 cggttggcga tccgtggcgt gccgcgcgaa cgcttggcga tctccagcgc gccagcctcc
      601 tgcatctcca cctccagcaa gccggctgat cgatgcacga tgcgacccaa ctcttccgag
      661 gtgtagaact ccaggcggga gacgataccg aagcggtcac gcaaagggtt ggtcagcatg
      721 ccggcgcgcg tggtcgcccc caccagggtg aacggcggca ggtcgaggcg caccgagcgc
      781 gccgccggac cctcgccgat catgatatcg agacggtaat cttccatcgc cgggtacagg
      841 atctcctcca ccacgggcga caaacgatgg atctcgtcga tgaacagcac gtcgttgggt
      901 tccagattgg tcagcagggc agcgaggtca ccggcacgct ccagcaccgg acccgaggtc
      961 tggcgcaggt taacccccat ctccttggcg atgatgtgcg ccagcgtggt cttgcccaac
     1021 cccggcgggc cgaacagcag cacatggtcc agcgcctcgc tgcgaccgcg cgcggcgttg
     1081 atgaagatct ccagttggcc acgcgctttt tcctggccga tatactcatc cagcagcttg
     1141 gggcgcagcg cacgttcgag cgcctcttct tgcgggcttt gcgcgtctgc ggcgatgagg
     1201 cggtcggttt cgatcatggc ttaggttttt gagagggtct tgagcgcgtg cttgatgccg
     1261 tccgctaccg tcgcctcggc aggcaactgc ttgacggcgg ccagcgcttc acgctcattg
     1321 tagccaagcg cgatcaatgc gttcaatacg tcgccggatg cggattttcc atggcccgcg
     1381 gcaccgggaa cggcgccatc gatggtgaat ttatccttga gctccagcag caagcgctct
     1441 gcggtcttct tgccgatgcc gggcacacgc gtcagcaatg ccggctcttg cagcgcgacg
     1501 gcctgcatca ggtcatcgac ggaaagcccg ctgaggatgg aaagcgccga cttggcgccg
     1561 atgccgttga ccttgagcaa ctggcggaaa gtcatgcgtt cggcatcgct accgaacccg
     1621 tagagcagtt gcgcgtcctc gcgcacgatg aactgcgtca gcaacaccac tttctcgccg
     1681 atggcaggca ggttgtagaa cgtgctcatc ggcacttcgc attcgtagcc gacaccattg
     1741 atgtcgatca acacctgcgg cggggtcttt tccagcagga tgccgctcag acgcccgatc
     1801 atgcttggcc acccttcata cgagccggcc tcccctgacc cggaagcctg cggtcgccag
     1861 cttgcccaac ccctgaccgc cgtgggcgtg acagatggcg caggccagtg catcagcgga
     1921 gtcgggtttg ggcaccccgg gaagattgag cagccgtttc accatttcct gcacttgctc
     1981 cttggcggcg tggccgttgc cgaccaccga ttgtttgact tgcaatgccg tatattctgc
     2041 cacaggcagg tgcttgatga ccgccgcact gatggcggcg cctcgtgcct gccccagcaa
     2101 aagcgtagat tggggattga cgttgacgaa cactttttcg accgcgacct gctggggctg
     2161 ataggtctca atgacctcgg ccagactttc caggatgatc ttgagccggg atggcagatc
     2221 ctcgatgtcg gcgcccttgc ccgtcgtggt cttgatggtg ccgctggcga cgtacgccag
     2281 tttctcgccg atcttttcga tgacgccgaa tcccgtcagt ctgaggccgg ggtcgatgcc
     2341 gaggatgcgc gtagtcaatg gctgcatatc atccgacagg ctggcgaacg gcacgccagg
     2401 caagctttgg cgctcgacgg aaactcaatg cccttgaaca gcaggcccca ggggccgctt
     2461 tgatgcgcgc aaaggtggat ggcgaagtaa ttttggggat tactcctcga tcaccgctgt
     2521 ggtgt
//
LOCUS       DATMTW010000345         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_398985, whole genome shotgun sequence.
ACCESSION   DATMTW010000345 DATMTW010000000
VERSION     DATMTW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398985"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VN084_06775"
     CDS             complement(<1..339)
                     /locus_tag="VN084_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HWT29199.1"
                     /translation="MTYCVALLLDGGLVFASDTRTNAGVDQVAVFPKMHVFEVKGERM
                     LTLLTAGNLGITQSVVNRLREAIKANEDRHLQNVGSMLEAAELVGDEMRRAYERDAEH
                     LKNHNAEFNAS"
     gene            complement(444..1682)
                     /locus_tag="VN084_06780"
     CDS             complement(444..1682)
                     /locus_tag="VN084_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29200.1"
                     /translation="MRHDKHPPLDSLAFTRHGDKQNSPFFEEYLTRLLEERDRTGLTA
                     MIHEIDALMITVDPGHSVQYIAELALMTPYHYLVTLESESHWTHVLRIDLESPDILVR
                     EVKDATLRGIFRSLNEVYPVGAKKPNSRYMGEIIRVSDLHDVVKLQHEREFRFFSQDE
                     VRKLELPGNLAVSKPSPYTHNIVAYLERKPDELRVYALGVSQIHAEAQAAYEQAKTLQ
                     IKLRLDDLLMPIDHLATRVYSQNREVAILEYLSWSSYHFWGAYDIKDQNSSTNVTKSV
                     HPVPESRSPAKVFTANNTPYFVNHLEHLPSPTETFVRNYGPRLHHMAIAVKDGERDGV
                     EHIDFVVNAIAEQGKGFLLDVIGSKDQGLKQIFSNASEHSSLIIEYVQRFGSFDGFFT
                     RENVAALTEAAGMQEGVKAL"
     gene            complement(1679..2482)
                     /locus_tag="VN084_06785"
     CDS             complement(1679..2482)
                     /locus_tag="VN084_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWT29201.1"
                     /translation="MELSIEHRTSYEYSESVNYTIQQLRLTPQDGFGQQVRHWEIRVN
                     GHLHRFDDAFGNAAHTLVLDNPHQAIHIVASGQVETGLAYSATEDRLPLGVYLRMTPL
                     TEVHGELARFAAAYQPHVGGDDSRWIDAMAEAIVMQVPYARGNTGVDTPASDAFILGQ
                     GVCQDHAHIFVACCRWLGLPARYVSGYLFTEDGSLMESHAWADVWLGEAGWRSIDVSN
                     RCPTGGQHVRLAVGLDYRDACPVSGMRVGGGLETMGANVRISQSEQLQQ"
     gene            complement(2482..>3312)
                     /locus_tag="VN084_06790"
     CDS             complement(2482..>3312)
                     /locus_tag="VN084_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWT29202.1"
                     /translation="SEAALWGPALRIAGDVIAYEAEYGPITAAGVIRYLAMDDENPSS
                     IFSSLRSARENARAVRVAMSSETWENINALWLEFSQFFGANLSEGGLHEFCDWVKSRS
                     HLFRGVSFGTMLRDDAFRFVRLGTFIERADNTARLLDVKYHLLLPSDEEVGGAVDYYE
                     WSAVLRSVSAFQAYQKVFSDSIEPWRVAELLVLRDDMPRSLHACLDEIVPILEQLSRR
                     NSECRRLAGEVHAKLHYGRMDEIFQEGLHEFLSDFIEYNNNLGFEIQRTFLNPPAVTN
                     "
BASE COUNT          667 a          972 c          996 g          677 t
ORIGIN      
        1 gctggcgttg aattcggcat tgtggttctt gaggtgctcg gcgtcgcgtt cgtaggcgcg
       61 gcgcatctcg tctcccacca gttcggccgc ttcaagcatg ctgcccacat tctgcaggtg
      121 cctgtcttca ttggccttga tcgcttcgcg cagacgattg accacggatt gcgtgatgcc
      181 cagattgcct gcggtcagca gcgtaagcat gcgctcacct ttgacttcga acacatgcat
      241 cttggggaac accgccacct gatcgacacc ggcattggta cgggtatcgg aggcgaacac
      301 aaggcctcca tcaagcaaca gggcaacaca ataggtcatg tcagcggcaa taggattcgt
      361 tgggagaaag agcctcaatc ataccagcca accgctcggc aaaacccttg cggagacaaa
      421 aatcagcccg gtgagtggtc gttctagagg gccttgaccc cttcctgcat gccagccgct
      481 tcagtcaggg cagcgacgtt ctcacgcgta aagaagccat cgaaactacc gaaacgctgc
      541 acgtattcga tgatgagcga tgaatgttcg gaagcgttgg agaagatctg cttcagcccc
      601 tgatccttgg agccgatgac atccagcagg aagcccttgc cctgctcggc gatggcgttg
      661 acgacgaaat cgatatgctc gacgccgtcg cgttcaccgt ccttcactgc gatcgccata
      721 tggtgcagcc gtggcccata gttgcgtacg aaggtctcgg tcggcgaagg cagatgctcc
      781 agatgattga cgaaatacgg ggtattgttg gcagtgaata ccttggccgg actgcgggac
      841 tccggtaccg gatgcacgct cttggtgaca ttggtggaag agttctggtc cttgatatcg
      901 taggcgcccc agaagtgata actcgaccac gacaggtatt cgaggatcgc cacctcacgg
      961 ttctggctgt agacccgcgt cgccaggtgg tcgatcggca tcagcaggtc gtccagacgc
     1021 aactttatct gcagggtttt ggcctgctca tacgcagctt gcgcttcggc atggatctgg
     1081 ctcacaccaa gcgcgtacac gcgcaattcg tccggcttgc gctcaagata ggcaacgatg
     1141 ttgtgcgtgt aaggtgaggg cttgctcacc gcaagattgc ccggcaattc gagcttgcgc
     1201 acttcatcct ggctgaagaa cctgaactcg cgttcatgtt gcaacttgac cacgtcgtgc
     1261 aagtcactga cgcgaatgat ctcgcccatg taacggctgt tcggcttttt cgcgccgacc
     1321 gggtagacct cgttgagact gcgaaagatg ccacgcaagg tcgcgtcctt gacttcgcgc
     1381 acgaggatat cgggcgattc gagatcgatg cgcagcacgt gcgtccagtg cgattcgctc
     1441 tccagagtga ccaggtaatg ataaggggtc atcagtgcca gttcggcgat gtattgcacc
     1501 gaatggccgg ggtccacggt gatcatcaac gcgtcgatct cgtggatcat ggcggtcaac
     1561 cctgtgcgat cacgctcttc cagcaggcgg gtcaggtatt cctcgaagaa gggcgaattc
     1621 tgcttgtcac catgccgcgt gaacgccaga gaatcaaggg gtggatgctt gtcatgcctc
     1681 attgctgcaa ctgctccgat tggctgattc taacgttggc gcccatggtt tccagaccgc
     1741 cgccgacacg catgccactc accgggcatg cgtcgcgata atccagcccc accgccaggc
     1801 gcacatgttg cccgccggta gggcaacggt tcgagacatc gatgctgcgc cacccagcct
     1861 cgcccagcca cacatcggcc caggcatgac tttccatcag gctcccgtcc tcggtgaaca
     1921 ggtaaccgct gacgtatcgc gcgggcagac ctagccagcg gcagcaggcg acgaagatgt
     1981 gggcatggtc ctggcagacg ccttgaccaa ggatgaaggc atcggaggct ggcgtatcga
     2041 ccccggtgtt acctcgggcg tacggcacct gcatgacgat ggcctccgcc atggcatcga
     2101 tccatcgact gtcatcgccg ccgacatggg gctggtaggc agcggcgaat cgcgccagct
     2161 cgccatgcac ttcggtcagt ggggtcatgc gtagatagac gccgagcggc aggcggtcct
     2221 ccgtcgccga atacgcgagg ccagtttcca cctgcccact cgcgacgata tgaatcgcct
     2281 ggtgcgggtt atccagcacc agggtgtgcg cagcattgcc aaaagcatcg tcgaaacgat
     2341 gcagatgtcc gttgacgcgg atctcccagt gcctgacctg ctggccgaaa ccgtcctgcg
     2401 gggtcagccg caattgctgg atcgtgtagt tgaccgactc gctgtattcg tagctggtgc
     2461 ggtgctcgat actgagttcc atcagtttgt gacagcgggt gggttaagaa aggtccgctg
     2521 gatctcgaaa ccgaggttgt tgttgtactc gatgaagtcg ctgaggaatt cgtgcaggcc
     2581 ttcctggaag atctcatcca tgcggccgta atgcaatttg gcgtgcactt cgcctgccag
     2641 cctgcgacat tcgctgttgc gacgggagag ttgctcgagg atgggcacga tctcatccag
     2701 acaggcgtgc agcgagcgtg gcatatcgtc gcgcagtacc agcaattccg ccacacgcca
     2761 gggttcgatg ctatcgctga agaccttctg gtaggcctgg aaggccgata cggaacgcaa
     2821 cacggcgctc cattcgtaat agtcgaccgc accgccgacc tcttcgtcgc tcggcagcag
     2881 cagatggtat ttgacgtcga gcaggcgcgc ggtattgtcg gcgcgctcga tgaaagtgcc
     2941 gagccgcacg aaacggaagg cgtcgtcgcg caacatggtg ccgaagctga cgccacggaa
     3001 caggtgcgag cgcgacttca cccagtcgca gaattcgtgc aaaccgccct cggataggtt
     3061 ggccccgaag aactggctga attccagcca tagcgcgttg atgttttccc aggtctcgga
     3121 ggacatggcg acacgcacgg cgcgggcgtt ctcgcgggcg ctgcgcaacg aactgaagat
     3181 gctggaggga ttctcgtcat ccatcgccag gtagcggatc acgccggcgg cagtgatcgg
     3241 gccatattcc gcctcgtagg cgatgacgtc tcccgcgatg cgcagggccg gaccccacaa
     3301 ggcagcctcg ct
//
LOCUS       DATMTW010000346         1934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_466820, whole genome shotgun sequence.
ACCESSION   DATMTW010000346 DATMTW010000000
VERSION     DATMTW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466820"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VN084_06795"
     CDS             complement(<1..455)
                     /locus_tag="VN084_06795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWT29203.1"
                     /translation="MALHPFSALIRKPQRSELLGLEYTAKSRYSGITDHALTVMLYLL
                     WGVIALVLVYTVRHYFFTLNRLFGEQRHPYLDIDTATWPSVSVLVAAHNEEAVIAEAL
                     QALLDVDYPADRLQIIPVNDRSTDRTQEIIDAIAAQHPGRLAPLHRRDGA"
     gene            complement(729..1562)
                     /locus_tag="VN084_06800"
     CDS             complement(729..1562)
                     /locus_tag="VN084_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWT29204.1"
                     /translation="MSLTQSLLGQMPGFAVQVLRLIIWLVLLSAIFVPLERLFALRPN
                     KRSFSETSQDLAYYFLSSLLPMLLLGLPLAIIAVAAYRLLPEVYLTAIGALPTTLRLL
                     LTFVIGEIGFYWGHRLSHEVSCLWRFHSIHHRPEHLYYLANTRAHPVDLIVTRLFGLV
                     PLYALGLVGPGVGGSAVPVALILVGTLWGFFVHSNLRLRMGPFEWLLSTPAFHHWHHS
                     RTDHINHNYASMLPVLDRVFGTHYLPATWPAQYGIQTEAPNTITEQLLTPFSGAARDR
                     R"
     gene            complement(1635..>1934)
                     /locus_tag="VN084_06805"
     CDS             complement(1635..>1934)
                     /locus_tag="VN084_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWT29205.1"
                     /translation="WRVNLGNDPSTWQATGIYTNASHSSSNWMQFSMVFTATSTSEML
                     SFLAAGTPSGEPPFSLLDGVSMYEVPEPASWLLMLLAMATIMAMRRRRRGCDTSR"
BASE COUNT          428 a          523 c          633 g          350 t
ORIGIN      
        1 gctccatcgc gccgatgcag cggcgcgaga cgccccggat gctgcgcggc aatcgcatcg
       61 atgatttcct gcgtgcggtc ggtcgagcga tcgttgaccg gaattatttg cagacggtct
      121 gccggatagt cgacatccag cagcgcctgc aaggcttcag cgatgacggc ctcttcgttg
      181 tgcgcggcga ccagaacgct gaccgaaggc caggtcgcgg tatcgatatc caggtaggga
      241 tgccgctgtt cgccgaacaa gcgattgagg gtaaagaaat aatggcgaac cgtgtacacc
      301 agcacgagcg cgattacccc ccacagcagg tagagcatca ccgtcagcgc gtggtccgtg
      361 atgccggaat aacgcgattt ggcggtgtat tcaagaccca gcaattcgct acgctggggt
      421 ttcctgatca gtgcagagaa ggggtgcagc gccaatgggt cgggcgtatg cgcagtcctt
      481 gcagatagga agtctgccgg ggcgacagca gtggcatccg tatctgcaac ggacgctgtc
      541 ggagctgatt cttgaacgtt ggcgcaatgg cccatcccga gccagcatga aaacgcgagc
      601 gcagcaagat atcggcccag gcggggcaac actgtcatcc ggccggcagt ggtcgaagtc
      661 atggacagaa agattattcg cattccgggg gcgtgttctt ggggagagct ggacctgggc
      721 cgtgaagttt atcgtctgtc tcgggctgcg ccggaaaaag gcgtcagcaa ttgttctgtg
      781 atggtgttcg gcgcttccgt ctggattccg tattgcgccg gccaggtagc gggcaggtag
      841 tgggttccga aaactcgatc gagcaccggc agcatcgagg catagttatg gttgatgtga
      901 tcggtgcgac tgtgatgcca gtggtgaaac gccggcgtgg acagcagcca ttcgaatggc
      961 cccatcctca ggcgcaggtt cgagtgaacg aagaatcccc acagcgtccc caccagaatc
     1021 agggcaacgg gaacggcgct gccgccgaca cccggcccga cgaggccaag cgcatatagc
     1081 ggcaccaggc cgaacaggcg cgtcacgatc agatcgacgg gatgtgcccg ggtattggcg
     1141 agatagtaaa ggtgttccgg cctatggtgt atcgagtgaa agcgccacag gcaggaaact
     1201 tcatgactca gacggtggcc ccaatagaaa ccgatttccc cgatgacgaa ggtcagcagc
     1261 agtctcagcg ttgtcgggag ggcgccgatt gccgtgagat agacctcggg tagcagccga
     1321 tacgcagcta ctgcgatgat cgccaatgga agcccgagca acagcatggg caacaggctg
     1381 ctgaggaagt agtaggcgag atcctgcgac gtttcgctaa aactccgctt gttcgggcgg
     1441 agcgcgaaga gacgctcaag cggcacgaaa atggcgctga gcagtaccaa ccagattatc
     1501 aaacgaagca cctgcactgc gaatccgggc atttgtccca gcagggactg ggtcagactc
     1561 atgaggaact ccgacttctg aaaatgaaag agccggtcga atcgcttcga ccggctttca
     1621 gcgcatacgg aaatctagcg cgacgtatcg caaccgcggc gtctgcgccg cattgccatg
     1681 atcgttgcca tggccagcag catcagcagc cagctggccg gctcggggac ttcatacatg
     1741 gatacgccgt cgagcagcga aaagggcggt tcgccggagg gcgtgcccgc ggcgaggaag
     1801 gacagcatct cgctggtgga ggtggcggtg aagaccatgc tgaactgcat ccagttactg
     1861 gagctgtgac ttgcgttggt atagatgccg gtggcctgcc aggtggaagg atcgttgccg
     1921 aggttgaccc gcca
//
LOCUS       DATMTW010000347         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_264553, whole genome shotgun sequence.
ACCESSION   DATMTW010000347 DATMTW010000000
VERSION     DATMTW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264553"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VN084_06810"
     CDS             <1..288
                     /locus_tag="VN084_06810"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29206.1"
                     /translation="DLDHALAAVREILDHNPRVLKMPPAVVNVRVLGASSISIAVKPW
                     IEVPDYVPAVGEINKAIVERFRVQGIAIPLPQREIRLLEGTSATPAGGKQP"
     gene            363..1316
                     /gene="hemC"
                     /locus_tag="VN084_06815"
     CDS             363..1316
                     /gene="hemC"
                     /locus_tag="VN084_06815"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWT29207.1"
                     /translation="MQPVAPKKLVIASRESALAMWQARHIQATLQALYPQTVVEILGM
                     TTTGDQILDSPLARIGGKGLFVKELEQALADGRADLAVHSMKDVPMHLPEGFAMAAIG
                     EREDPRDAFVSNDYERLQDLPAGSIVGTSSLRRQSQLQARFPDLRIESLRGNLQTRLR
                     KLDDGQYAAIILAAAGLKRLGLAARIRQPIATEDSIPAVGQGALGIEINAARTDLLPI
                     LAPLNHADTAACVRAERGMSRALAGSCTVPLGAYAEKRGERLHMTGFVASVDGREFLR
                     ESIAGDAETPETLGQQLADRLLARGADRILAALADATHDSR"
     gene            1303..2085
                     /locus_tag="VN084_06820"
     CDS             1303..2085
                     /locus_tag="VN084_06820"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWT29208.1"
                     /translation="MTVGKLQDRHIVITRPPGQASRLSRLIVAQAGIAHLFPLIEIVP
                     LEDYTAFDGVIAALEDYDWAIFISSNAVEHAMPRLLARSAGMPSGLRFAAIGPVTATS
                     LAGYGVTQTLTPSQRFDSEALLALPEMQAVAGRKILIFRGVGGREVLAETLRARGASV
                     TFAECYRRINPQRNADSLQQLWQNGSLDAIVVTTSEAMRHLLALAGDNAPWLAATPLC
                     VNHARIGELASSYGLTTAVADAPGDDAMLACLIRTMDHPKDR"
     gene            2096..3175
                     /locus_tag="VN084_06825"
     CDS             2096..3175
                     /locus_tag="VN084_06825"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWT29209.1"
                     /translation="MTEQTPSTENISEASPSQSRRSSLFARVALLLVIVALLTLAWQW
                     LNTRHRFNQVERALSERLEQLQAINQQSLALARNADERSIETAARSAQLEQRLAESQD
                     QQEALQTLYMELANNREERLISEVEQLLIIANQQLQLVGNIKSALIALQTADSRLQQL
                     DTPQVIQLRKQVSQDLQHLQSLPTVDIVGMSLKLANLAESIDTLPLVSERHPTSSRPP
                     QPAWQGSPLRQFAQELWHDLKQMVRIERVDRPEPPLLAPDQTFFLRENIKLRLLTARI
                     ALLQHDEASYRNDLDAARTWLNSHFDQREQATQSALHTLEQLSANDISIQLPDISDSL
                     ALVSKYKLTLERKSAGTTPPAERKR"
BASE COUNT          642 a         1106 c          940 g          544 t
ORIGIN      
        1 gatcttgacc acgccctggc cgccgtccgc gagatcctcg accacaaccc gcgcgtcctc
       61 aagatgccgc ctgccgtggt caatgtcaga gtgctcggcg cctcctccat cagcatcgcg
      121 gtcaaacctt ggatcgaggt gccggattat gtgccggccg tgggggaaat caacaaggcc
      181 atcgtcgagc gcttccgggt gcaaggcatc gccatcccct tgccgcagcg cgagatccgc
      241 ctgctggaag gcacttcggc aacgcccgca ggcggtaagc agccctagcc ctgtgttaaa
      301 atccgggctc tctccatcaa cgcttttggt cttaagtgaa tcctgccgcc gtgaacgccg
      361 ccatgcaacc tgtcgctccg aagaaactgg tcatcgcctc gcgcgaaagc gcgcttgcca
      421 tgtggcaagc acgccatatc caggcgacct tgcaagccct ttacccgcaa accgtcgtcg
      481 agatcctcgg catgaccacc acaggtgacc agatcctgga ctcgccgctg gcccgcatcg
      541 gcggcaaggg gctgttcgtc aaggagctgg agcaggcgct ggccgacggt cgtgccgatc
      601 tggccgtgca ttccatgaaa gacgtcccca tgcacctgcc ggaagggttc gccatggcgg
      661 ccatcggcga acgcgaagac cctcgcgatg cattcgtctc caatgattac gaacgcctgc
      721 aagacctgcc cgcaggcagt atcgtcggca cctcgagcct gcgccgccag agccagctgc
      781 aagcccgctt ccctgacctc aggatcgagt cgctgcgcgg caacctgcag acgcgtctgc
      841 gcaaactcga tgacggccaa tatgccgcca tcatcctggc tgccgcgggc ctcaaacgcc
      901 tcggattggc ggcacgtatc cggcaaccga tcgccaccga agacagtatc ccggccgtcg
      961 gccaaggtgc gctgggcatc gagatcaatg ccgcacgcac ggatctgcta cccatccttg
     1021 cgcctctcaa ccacgccgat accgcagcct gcgtgcgcgc cgagcgcggc atgagccggg
     1081 ccctggcggg aagctgcacc gtgcccctgg gcgcctacgc cgagaaacgt ggcgagcgcc
     1141 tccacatgac gggtttcgtc gctagcgtcg acggccggga gttcctgcgt gaaagcatcg
     1201 cgggtgatgc cgaaaccccg gagacgcttg gccagcagct ggctgaccga ttgctcgccc
     1261 gcggcgcgga tcgcatcctc gccgcactgg ccgatgcgac gcatgacagt aggtaagctg
     1321 caagaccgtc acatcgtcat cacccggcct ccaggccagg ccagccggtt gagccggctg
     1381 atcgtggcgc aagcgggtat tgcccacttg ttcccgttga tcgagattgt gcccttggag
     1441 gactacacgg cgttcgatgg ggtgattgcc gcgctcgaag actacgactg ggcgatcttc
     1501 atcagttcca acgccgtcga gcatgccatg ccgcgcctgc tggcacgttc ggctggcatg
     1561 ccctccggct tgcgcttcgc cgcgatcggt cccgtcaccg cgaccagtct cgctggctac
     1621 ggggtgaccc aaaccctgac accgtcgcag cgcttcgata gcgaagccct gctcgcgcta
     1681 ccggagatgc aggccgttgc cggacgcaag atcctgatct tccgtggcgt cggcggccgc
     1741 gaagtattag cggagacctt gcgcgcgcgc ggcgccagcg tcaccttcgc tgaatgttac
     1801 cggcgcatca atccacagcg gaatgccgac agcctgcagc agctatggca gaatggaagc
     1861 ctggacgcca tcgtcgttac caccagcgag gccatgcggc acctgctcgc gctggccggc
     1921 gacaacgcgc catggttagc ggctacgccg ctttgcgtca atcatgcacg catcggcgag
     1981 ctggcgagca gctacggcct gaccaccgcg gtggccgatg cccctggcga cgacgccatg
     2041 ctggcctgcc ttatccgcac catggaccat cctaaggatc ggtagagaac cacgtatgac
     2101 agaacaaaca ccttccacag aaaacatttc cgaagccagt ccaagccagt cacggcgttc
     2161 ttccttattc gcccgcgtgg ccctgctact ggtgatcgtc gcgttactga cgctggcctg
     2221 gcaatggctc aacacgcgcc accgtttcaa ccaggtcgaa cgtgcgctga gcgaacgcct
     2281 ggagcaactg caggcgatca accagcaaag cctggcgctt gcccgaaatg ccgacgaacg
     2341 cagcatcgag accgcggcac gcagtgcgca actggagcaa aggctagctg aatcacagga
     2401 ccagcaggag gccctgcaga cgctttacat ggaactcgcc aacaaccgcg aggaacgcct
     2461 gatctccgaa gtcgagcaac tgctgatcat cgccaaccag cagctacaac tggttggcaa
     2521 catcaaatcc gcgctgatcg cgctgcagac ggccgacagc cgcctgcagc aactcgatac
     2581 gccccaggtc atccagctgc gcaaacaggt gagccaggac ttgcagcatc tgcagagcct
     2641 gccaacggtg gatatcgtcg gcatgagcct caagcttgcc aacctcgccg agagcatcga
     2701 tacgctgccg ctggtgagcg aacgccatcc gaccagtagc cggccaccgc agccggcgtg
     2761 gcaaggtagc ccgctacgcc agttcgctca ggagctatgg cacgacctca agcagatggt
     2821 ccgcatcgaa cgcgtcgacc gtccggagcc gcctttgctt gcgcctgacc agaccttttt
     2881 cctgcgcgag aacatcaagc tgcgcttgct gacggcacgc atcgccttgc tgcaacacga
     2941 tgaagccagc taccgcaacg atctcgatgc ggcccggacg tggctcaaca gccattttga
     3001 ccaacgtgag caggcaaccc agagcgcgct tcacaccctt gagcagctat ccgccaacga
     3061 catttcgatc cagttaccgg acatcagcga cagccttgca ctggtgagca agtacaaact
     3121 gaccctggag cgcaagtcag ctggcacgac cccgcctgct gagcggaaac gataatgcgc
     3181 tggctgttct ggatcctgtt gacgctggcg ctcgccatcg gcgtatcgat gg
//
LOCUS       DATMTW010000348         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_433296, whole genome shotgun sequence.
ACCESSION   DATMTW010000348 DATMTW010000000
VERSION     DATMTW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433296"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VN084_06830"
     CDS             <1..1156
                     /locus_tag="VN084_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HWT29210.1"
                     /translation="MCSRADFARWAGKGKSLRMEFFYRWMRKRHDVLMEDGAPVGGVW
                     NFDADNRQSFGKRGPQDLPAMPPVNIDAVTHAVLAEVEHQFPEHPGSPAHFVWPVTRA
                     QALQFLDDFIRYRLAGFGPHQDAMWQSPDGAPTPFLWHSLLSSSLNLKLLDPREVIAA
                     AEQAWRQGDLPLASVEGFIRQVLGWREFIRGVYWLDMPQMASDNHYGHARALPAWYWT
                     GDTGMNCLRQTIRQTLQYGYAHHIQRLMVTGLFGLLAEIDPRQVEAWYLAVYVDAVEW
                     VELPNTAGMALYANGGRFTSKPYVASGAYIKRMSNYCDGCRYRPEVKTGPQACPLTTL
                     YWHFLIRHHAAFANNPRTALMAKNVDRMSAEQQAAIQQYASQLLQKLESL"
     gene            complement(1202..1696)
                     /locus_tag="VN084_06835"
     CDS             complement(1202..1696)
                     /locus_tag="VN084_06835"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HWT29211.1"
                     /translation="MTELRHRLVKRSIRAIAIFEGGKGLLALIWTTLFISVFHADAHR
                     LAELLIRDLHYDPMDYYPHLLLMIADTFTGSRLIMLIACAYLYALIKTSEGYGLWRER
                     EWGRTLGILSIGVLIPFEVYENVQHASLGKFTVLAINVGIVAILFLGMGRVTARNASH
                     PDQR"
     gene            complement(1741..3375)
                     /locus_tag="VN084_06840"
     CDS             complement(1741..3375)
                     /locus_tag="VN084_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWT29212.1"
                     /translation="MKPQFQPARQPSQMEVQPLLNLLNSGQLPQAEHMAKSLLQRYPT
                     AFILHNVLGLALEGQQRYPEAEQAYRKTIQIDGKIGEIHFNLGVVLANQGKSEEAIGS
                     YRKAIALKPNLAVAHFNLGIALQGLGRNEEATAAYRKAIGYEPGFYEACGNLGAVLQA
                     QGRLEDAVSAYRKAIAIHADARGYFNLGTALRNQGAAQEAISCFNQALALDPRFVDAW
                     TNLGDVFFHQGKANEALDCYGKALSIDPSHPATNYNLGIFYYDGGELEKAIPCFQAAG
                     IADWRERTLYCLYKTGQYDAFREGMQAMLNEPHLSPFLATLSTHYATNFGVEDVYDFC
                     KTPMDFVFHSQITELAGEDSALRAELIRDIEQAEIAERKQGRLHNGIQSSGNLFKRPE
                     ASFRKLAELVKRQVQAYQAAFAGADNSLMRHFPREIEFSSSWYVKMRQGGHLTSHIHE
                     EGWLSGSLYLSMPTRAPGSDAGSIEFSTHGDDYPQQHTDFPRRAIAPSVGDIVIFPSS
                     LFHRTIPFEADEARICIAFDIRPNEPSSVSSLMSGS"
     gene            3526..>4794
                     /gene="htpG"
                     /locus_tag="VN084_06845"
     CDS             3526..>4794
                     /gene="htpG"
                     /locus_tag="VN084_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HWT29213.1"
                     /translation="MSTDTTKKKAAGQQKMAFQAEVKQLLQLMIHSLYSNKEIVLREL
                     ISNASDAADKLRFEALSNNVLYENESELKIRIAFDKASRTVTISDNGIGMSRQEVIDN
                     IGTIAKSGTKEFFQSLTGDQARDANLIGQFGVGFYSAFIIADKVTLITRRAGEQEAVK
                     WESAGEGDFTLEAAEKPTRGTDIVLHLREGEDEFLNDWKLKSIIRKYSDHITLPIVMK
                     ASEWKDGEQVPTDEDETVNKASALWARNKNDITEEEYKEFYKHVSHDFEEPLAWSHSR
                     VEGKQEYISLLYIPTQAPFDLYDRERRHGIKLYVKRVFIMEDAEKLMPQYLRFVRGVI
                     DSADLPLNVSREILQHSKDIDAIKAGSVKKVLGLLEDLAENKPEEYAKFWNAFGRVLK
                     EGPGEDFANKDKIAGLLRFASTHLDTEEQSV"
BASE COUNT          977 a         1407 c         1478 g          932 t
ORIGIN      
        1 catgtgcagt cgtgccgact tcgcccgttg ggccggcaag ggcaagagcc tgcgcatgga
       61 gttcttctac cgctggatgc gcaagcggca tgatgtgctg atggaggatg gggcaccagt
      121 gggcggcgtg tggaatttcg atgccgacaa ccggcaatcc ttcggcaagc gcgggccgca
      181 agacttgcct gccatgccgc cagtcaacat cgatgccgtc acgcatgccg tgctcgccga
      241 ggtcgaacat caatttcccg agcatccagg cagccctgcg catttcgtct ggcccgtgac
      301 gcgtgcacaa gctctgcagt tcctcgatga tttcattcgc tacaggctcg ccggtttcgg
      361 cccgcatcag gacgccatgt ggcaaagccc ggacggcgcg ccgaccccct ttctctggca
      421 ttccctgcta tcgagctcgc tcaacctcaa actgctcgat ccgcgcgagg tgatcgcagc
      481 agcggaacag gcctggcgcc agggtgacct gccgctggcc agtgtcgaag ggttcatccg
      541 tcaggtgctg ggctggcgcg aattcatccg cggggtgtac tggctggaca tgccgcagat
      601 ggcgtcagac aaccattacg gccacgctcg cgccttgccg gcctggtact ggaccggcga
      661 caccggtatg aactgtctgc gccagaccat ccgtcagacg ctgcaatatg gctacgcgca
      721 tcatatccag cggctcatgg tcacgggtct gttcggcttg ctggcggaga tcgatccgcg
      781 gcaggtggag gcttggtatc tggcggtgta tgtggatgcg gtggaatggg tggagttacc
      841 caacaccgcc ggcatggcac tctatgccaa cggcggccgc ttcaccagca aaccctatgt
      901 cgccagcggc gcctatatca agcgcatgag caattactgt gacggctgcc gctaccggcc
      961 agaggtcaaa accggcccgc aagcgtgtcc cctgacgacc ttgtactggc atttcctgat
     1021 tcgtcaccat gcggcatttg ccaacaatcc gcgtaccgcg ctcatggcga agaacgtcga
     1081 ccgcatgtca gccgaacagc aggcggcgat ccagcaatat gccagccaat tgctgcagaa
     1141 gctggaaagc ctctaaggcc aggcggcggc aactgccggc gcattggcgg tgaacccttg
     1201 gctagcgttg gtcggggtgg ctggcgttgc gggcagtcac tctgcccatg ccaaggaaca
     1261 ggatggcgac gatgccgaca ttgatcgcca ggaccgtaaa tttgccgagg ctggcgtgct
     1321 gcacgttctc atagacctcg aacgggatca gcacgccgat actcaggatg ccgagcgtac
     1381 gtccccattc gcgctcacgc cacaaaccgt agccttcgct ggtcttgatg agcgcataga
     1441 gataggcgca ggcgatgagc atgatcagtc ggctgccagt gaaggtgtcg gcgatcatca
     1501 acaacaggtg cggataataa tccatgggat cgtaatgcag gtcgcggatc aatagttcgg
     1561 ccagacgatg cgcatcggca tggaagaccg agatgaacaa cgtcgtccag atcagcgcca
     1621 gcagcccttt gccaccctca aagatggcga tggcgcgaat gctgcgcttg accaggcggt
     1681 gccggagttc ggtcatcgga tcgtccccga tccttgccgt ggtcgatcca tcgccggcac
     1741 tcagctgccg ctcatcaggc tagagacgct ggaaggctcg ttgggccgga tatcgaaagc
     1801 gatgcagata cgggcttcgt cggcttcgaa cgggatggtg cggtggaaca gcgatgacgg
     1861 gaagatgacg atgtcgccga cgctgggcgc gatggcgcgc cgcgggaaat cggtgtgttg
     1921 ctgggggtaa tcgtcgccgt gcgtgctgaa ctcgatgctg ccggcgtcgc taccgggcgc
     1981 acgcgtcggc atcgacaggt agagcgagcc gctgagccag ccttcctcat ggatgtgtga
     2041 ggtgagatgg ccgccctggc gcatcttgac gtaccaagaa ctgctgaact cgatctctcg
     2101 cgggaaatgc cgcatgaggc tgttatcggc gccggcgaac gctgcctgat aagcctgtac
     2161 ctggcgtttg acgagttcag ccagcttgcg gaacgaggct tccggacgct tgaacaaatt
     2221 accggatgac tggatgccgt tgtgcagtcg gccttgtttg cgttccgcga tctcggcctg
     2281 ctcgatgtcg cggatcagct cggcgcgaag tgcgctatct tcaccggcga gttccgtgat
     2341 ctggctgtgg aagacgaaat ccatcggggt cttgcagaaa tcgtagacat cttctacccc
     2401 gaaattagtc gcgtagtggg tcgacagggt tgccaggaac ggcgataggt ggggttcgtt
     2461 cagcatcgcc tgcatgccct cgcggaaagc gtcgtactgc ccggtcttgt agaggcaata
     2521 caaggtgcgc tcgcgccagt cggcgatgcc cgccgcctgg aagcagggga tggctttttc
     2581 cagctcgccg ccgtcgtaat agaagatgcc cagattgtaa ttcgtcgccg gatggctggg
     2641 gtcgatgctc aacgccttgc cataacagtc cagcgcttcg ttggccttgc cctgatggaa
     2701 gaacacgtcg ccgaggttgg tccaggcatc gacgaatctg ggatcgagcg ccagtgcctg
     2761 gttgaagcaa ctgatggctt cctgcgcggc gccctggttg cgcaatgcag tgccaaggtt
     2821 gaaatagccg cgtgcgtcgg cgtggatggc gatggcttta cggtaggcgc tgaccgcgtc
     2881 ttccagcctg ccctgggcct gcagcacggc gcccaggttg ccgcaggctt cgtagaagcc
     2941 aggctcataa ccgatggcct tgcggtaggc ggccgtggct tcttcgttgc ggccaagacc
     3001 ttgcagggcg atcccgaggt tgaaatgcgc gacggcgagg ttgggcttga gcgcgatggc
     3061 cttacggtag ctgccgatgg cttcctctga tttgccctgg ttcgccagta ccacgcccag
     3121 attgaaatgg atctcgccga tcttgccgtc gatctggatg gtcttgcgat aggcctgttc
     3181 ggcctcggga tatcgttgct gcccttccag cgccaggccg agcacgttat gcaggatgaa
     3241 tgccgtcggg tagcgctgca acaaggactt ggccatatgc tctgcctgcg gcaactggcc
     3301 gctattgagc aggttgagta gcggttgcac ctccatctgc gaaggctgcc gggctggctg
     3361 gaattgcggt ttcatcgata tccccgtgcg tagttgcccc acattctaaa ctgaatgcgg
     3421 cggcacttga acacggccgc ggccgcgcct gacgacatct cttgaattcc atggcagcga
     3481 cctcaaatac agggctatct ttatctgaca atcacgggag aagacatgtc cacggatacg
     3541 accaagaaga aagctgcggg ccagcaaaag atggctttcc aggccgaggt caagcagctg
     3601 ctgcaattga tgatccattc gctctacagt aacaaggaga tcgtgctgcg cgagctcatc
     3661 tccaacgctt ccgacgccgc cgataaactg cgtttcgaag ccctgagcaa caacgtctta
     3721 tacgagaacg agagcgagtt gaagatccgc atcgcgttcg acaaagccag ccgcaccgtc
     3781 accatctccg acaacggtat cggcatgagc cgtcaggaag tcatcgacaa catcggcacc
     3841 atcgccaaat ccggtaccaa ggaattcttc cagtccctca cgggcgacca ggcaagggac
     3901 gccaacctca tcggccagtt cggtgtcggc ttctattccg ctttcatcat cgccgacaag
     3961 gtcacgctca tcacccgccg tgccggcgag caggaagccg tcaaatggga gtcggcaggc
     4021 gagggcgact tcacgctcga agctgccgag aaaccgacgc gcggtactga tatcgtcctg
     4081 catctgcgcg aaggcgagga cgagttcctc aacgactgga agctcaagtc catcatccgc
     4141 aagtattccg accacatcac actacctatc gtcatgaaag cttccgaatg gaaggacggc
     4201 gagcaggtgc ccacagacga ggacgagacc gtcaacaagg catctgccct gtgggcgcgc
     4261 aacaagaacg acatcaccga agaggagtac aaggaattct acaagcacgt atcgcatgac
     4321 ttcgaggagc cgctggcctg gagccacagc cgtgtcgaag gcaagcagga atacatctcg
     4381 ctgctctata tcccgaccca ggcgccgttt gacctttatg accgcgagcg ccgccatggc
     4441 atcaagctct acgtgaaacg cgtgttcatc atggaagatg ccgagaaact gatgccgcaa
     4501 tacctgcgct tcgtgcgggg ggtgatcgat tccgccgacc tgccgctcaa cgtttcgcgc
     4561 gagatcctgc agcacagcaa ggatatcgac gccatcaagg cgggttcggt gaagaaggtg
     4621 ctgggcctgc tggaagacct ggccgagaac aagccggaag aatacgccaa gttctggaat
     4681 gccttcggcc gtgtgctgaa ggaaggcccg ggcgaggact ttgccaacaa ggacaagatc
     4741 gccgggctcc tgaggtttgc ttccacgcat ctggataccg aggagcagtc ggtc
//
LOCUS       DATMTW010000349         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_366879, whole genome shotgun sequence.
ACCESSION   DATMTW010000349 DATMTW010000000
VERSION     DATMTW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366879"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3096
                     /locus_tag="VN084_06850"
     CDS             <1..3096
                     /locus_tag="VN084_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWT29214.1"
                     /translation="TRPIFASVLSIIIVLAGFAASMKLPIAQYPQIAPPTVIITATYP
                     GASAETLSKTVAAPIEEQLSGVEGLLYFNSSADSSGTLQITATFEIDTDVDQATFNVS
                     NRVNIALPRLPEDVRRTGVIVQKRSNDILLVVMLTSPSKKFEPLYLSNYATLNVLDEL
                     KRIKGVGDATIFGAQDYSMRIWLRPDKMAQLGVTTTEIADAIRAQNAQYAAGKIGQDP
                     APSDQQIVYTVTAKGRLVDPEQFGNIIVRADGPRGVLYLKDVARIELGSQNYNVRTAL
                     GGQPGVAIPIFLQSGANALDTAKAIKAKMDELKDRFPEGMEYTIPYDTSDFVKASIKE
                     VVKTLGEAMVLVVLVVFLFLQSWRATLIPIIAVPISLIGTFAGLWVFGFSINTLTLFA
                     MVLSIGIVVDDAIVVLENIERLMSEEKLSPVNAAIKSMQQVSGAVVAIVLVLCAVFVP
                     VAFLGGIAGELYRQFAVTVAVAVVISGIVALTLTPALCSLILRSVHGESAFFRPFNNA
                     FSRLTRLYMRVVDLTLHHRIIGAVVFAAIIALAVVMLRSVPGSFVPPEDQGYVVTAVI
                     LPDGATLARTSKTTESIRAEIDKDPAVWTEFAVNGFDLIGGGNKTSSATMFVRLKDWD
                     QRKTTAEEIVKKLIGIGMKQTDGMAIAFNPPAIRGLGTSGGFELYVQSRGDTNPMKLS
                     ATVNNFMDALRKEPRLTGINTFFRPTVPQLFVEVDEAKAISLGVPVSDIYATLQTTMG
                     SLYVNDFNRSGRTYRVQLQAEPEFRTKPEDLGKVYVRSKTGVMVPMSALSTVTSIVGA
                     EQLERYNGLVSAKVLGSGAPGVSSGDAIKLVEKIAKENLPDEYQIAWTGQAYQEKRTG
                     SAAIFAFSFAIIMVFLILAAQFETWALPLAVIMAVPFALTGALLAVFVRGMPNDIYFQ
                     IGLITLIGLAAKNAILIVEFASQKLEEGMPIMEAAVEAARLRFRPIVMTSMAFVLGIV
                     PLVVATGAGSAARRSMGTGVFGGMLLATFVATIFIPLFFTWLTGKKKEKGAEHAGKGG
                     V"
BASE COUNT          619 a          924 c          963 g          686 t
ORIGIN      
        1 accaggccga tcttcgcatc ggtgctgtcg atcattattg tcctggccgg ctttgcggcc
       61 tcgatgaagc tgccgatcgc gcaatatccg cagattgctc cgccaacggt catcatcact
      121 gcgacctatc cgggcgccag tgccgagacg ctatcgaaga cagtggccgc gccaatcgaa
      181 gagcagctca gcggggtcga aggtttgctt tacttcaact cgagcgccga ctccagcggc
      241 accttgcaaa tcactgccac attcgagatc gacacggatg tggaccaggc gaccttcaac
      301 gtcagtaacc gcgtcaatat cgctttgcct cgcctgccgg aagatgtgcg gcggacaggc
      361 gtgatcgtgc agaagcgttc gaacgatatt ctgctggtcg tcatgctgac gtctccgagc
      421 aagaagttcg aaccgctgta tctcagcaac tatgcgaccc tgaacgtgct ggatgaattg
      481 aagcgtatca agggtgtggg cgacgctacg atcttcggcg cgcaggacta ctccatgcgg
      541 atctggctgc ggcctgacaa gatggcgcag ctgggtgtga cgacgacaga gatcgcagac
      601 gccattcgcg cgcagaacgc gcagtacgct gccggcaaga tcggccagga ccccgcgccc
      661 agcgatcagc agatcgtcta caccgttacg gctaaaggcc gcctggttga tccggagcag
      721 ttcggcaaca tcatcgtccg cgctgatggc ccacgtggtg tgctttacct gaaggatgtc
      781 gctcgtatcg agctcggttc tcagaactat aacgtgcgca cagcgctcgg cgggcagcct
      841 ggcgttgcca tcccgatctt cctgcagtct ggcgccaatg cgctcgatac ggccaaggcc
      901 atcaaggcca agatggatga gcttaaggac cgtttcccag aggggatgga atacaccatt
      961 ccttacgaca ccagcgattt cgtcaaggct tcgatcaagg aggtcgtcaa gacactgggc
     1021 gaagccatgg tgctggtcgt gctggtcgtg ttcctcttcc tgcagagctg gcgtgcgacg
     1081 ctgatcccga tcatcgccgt gcccatctcg ctgatcggta cctttgccgg tctgtgggtc
     1141 ttcggcttct cgatcaatac cctgacgcta ttcgccatgg tgctgtcgat cgggatcgtg
     1201 gtcgacgatg cgatcgtcgt gctggagaac atcgagcgct tgatgtccga agagaagctg
     1261 tctccggtca atgccgcgat caagtctatg cagcaggtat ccggggccgt ggtcgctatc
     1321 gttctcgtcc tgtgtgctgt gtttgtgcca gtcgcgttcc tcggcggtat agcaggcgag
     1381 ctctatcgtc agtttgcagt gacggtagcg gttgcggtcg tcatctccgg catcgttgcg
     1441 ttgacgctga caccggcatt gtgctcactg atcctgcgct cggtgcatgg cgagtcggcc
     1501 ttcttccgac ccttcaataa cgccttctcg cgcttgacac gtctctatat gcgcgtcgtc
     1561 gatctgacgc tgcatcaccg catcatcggt gccgtcgtct ttgccgcgat catcgccctg
     1621 gcggtggtga tgttaaggag cgtgcccggc agcttcgtgc cgccggaaga ccaggggtat
     1681 gtcgtcacgg ctgtgatcct gccggatggc gcaacattag cccgtacgtc caagaccaca
     1741 gaatccatcc gtgcagagat cgacaaggat ccggccgtct ggaccgaatt cgcagtcaat
     1801 ggattcgacc tgatcggtgg cggtaacaag accagctctg cgaccatgtt cgtgcgactg
     1861 aaggactggg accagcgcaa gacgaccgcg gaagagatcg tcaagaagct gatcggcatc
     1921 ggcatgaagc agaccgacgg catggcgatc gccttcaacc cgccagccat tcgtggcctg
     1981 gggacttccg gtggcttcga actgtatgtc cagagtcgtg gcgacaccaa cccgatgaag
     2041 ctgtcggcga ccgtcaacaa cttcatggac gcattgcgca aggagccgcg cctgaccggc
     2101 atcaatacct tcttccgtcc cacggtgccg cagctgttcg tcgaagtgga tgaggccaag
     2161 gcgatctcgc tcggcgtacc ggtctctgac atctacgcta cgctgcaaac cacgatgggt
     2221 tcgctttacg tcaacgactt caaccgttca ggacgtactt accgtgtaca actccaggca
     2281 gagcctgaat tccggaccaa gcctgaagac ctcggcaagg tctacgtgcg gtcgaagacc
     2341 ggcgtcatgg tgcccatgtc cgcactgagc acggtcacta gcatcgtcgg tgccgagcag
     2401 ctcgaacgct acaacggcct cgtctcggcc aaggtgctgg gtagtggcgc gccgggtgtg
     2461 agctcgggtg atgcgatcaa gctggtggaa aagatcgcca aggagaacct gccggatgag
     2521 taccagatcg cctggaccgg tcaggcttat caggagaagc gtacgggttc tgcggcgatc
     2581 ttcgccttca gtttcgccat catcatggtg ttcctgatcc tggcggcaca attcgagacc
     2641 tgggcgttgc cactggccgt catcatggcg gtgccgttcg cgttgacagg cgcattgctg
     2701 gcggtattcg tgcgtggcat gccgaacgac atctatttcc agatcggctt gatcacgctg
     2761 atcggtctgg cagccaagaa cgcgatcctg atcgtggagt tcgccagcca gaagctggaa
     2821 gagggcatgc ccatcatgga ggctgcggtc gaagccgcgc gcttgcgctt ccgcccgatc
     2881 gtcatgacgt ccatggcgtt cgttctgggg atcgtgccgc tcgtggttgc taccggcgcc
     2941 ggctcggctg cgcgtcgttc gatgggtacg ggggtgtttg gaggcatgtt gctggcaacc
     3001 ttcgttgcca ccatcttcat tccgttgttc ttcacatggt taacaggcaa aaagaaagaa
     3061 aagggtgctg agcatgcagg aaagggtggc gtatgaagcc taatatgaaa cttctgcagc
     3121 ttgcggtcgc aatcacgctt ttgcctggat gcatgctgat gggcccggat tataaaaggc
     3181 cggaaaccca gg
//
LOCUS       DATMTW010000350         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_668618, whole genome shotgun sequence.
ACCESSION   DATMTW010000350 DATMTW010000000
VERSION     DATMTW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668618"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN084_06855"
     CDS             <1..275
                     /locus_tag="VN084_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWT29215.1"
                     /translation="EGIAERADLGGLSFDRAALVLSAAADGLGIALESTRLAETELRQ
                     GSLVVLNGHQPPLQREMHFLCYRGSMKGSRKIERFKEWIMNQIHEA"
     gene            complement(319..1272)
                     /locus_tag="VN084_06860"
     CDS             complement(319..1272)
                     /locus_tag="VN084_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWT29216.1"
                     /translation="MYTVLAIVLPIFALILAGFICRKTNRLGDQAASEINKMVVWLCL
                     PALLFKVTATASWAEIWQPGFIISFGAGTLAIFVATLIWRLSTGHSLATSSIDGLSAG
                     YANTGYIGIPLCAIVFGHPGLQPALISTIIVVCLLFAIAVICIEIGLQEERHLGRAVG
                     VVVKALSKNPLVIAPIAGAVWAMTGIPLAHPVMSFLDMLATATTPCALVSLGLFLGQK
                     TVKSTGGKSNPWPLVTIKLVGQPLLTWFLAFKVFALQSMWANSALLLSALPTGTGPFM
                     LAEFYRREAGLISRTILISTLGSLATLTGLMIWMNTGIAGA"
     gene            1343..2263
                     /locus_tag="VN084_06865"
     CDS             1343..2263
                     /locus_tag="VN084_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWT29217.1"
                     /translation="MKYQIDTSGASMIEHRHLRYFLALADHLHYGKASAQVNVAQPSL
                     SRQIADLESQLGCRLFARTSRSVELTAAGSEFQRSAQLVIQAMDSAIRSTQAVARGEK
                     GELRIAFTSMIAWSGLPKMIKSYAVNAPGVNLELSELIPGALMKAVETGECDIGLTFK
                     ATVQPPYQYRELQRESLSIAVPGDHPLARKKSVTVQDLIDEPFILSPRDTASALHDTI
                     FKLCRDSGFEPRVRMYTQLQGTIVNLVGEGLGVSIIPTSMSKSNPDSVLFIPITNAPE
                     ITLGMVWNMENSNPCLSAFIQSVGTRLDPP"
     gene            complement(2292..3605)
                     /locus_tag="VN084_06870"
     CDS             complement(2292..3605)
                     /locus_tag="VN084_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWT29218.1"
                     /translation="MPNALDAACVPDSRSIPDESKLYARLTLRLIPFLFLCYVFAYLD
                     RVNVGFAKLQMLDELKFSETVYGLGAGIFFFGYFIFEVPSNMMLHKIGARRWIARIMV
                     SWGVLSACMVFVQTPAMFYMLRFLLGVAEAGFFPGIILYLTYWFPSSRRGKITAMFLT
                     GIPMAGVLGGPLSGWIMSTTHGWHGYSAWQWLFALEAIPSIIAGFVVLFYLRDNPDDA
                     KWLTTEEKAVLKRNLQGEDQSHHGHSAFSAFTNPKVWLLSAGYFGVMMGLNGITFWLP
                     SLIKNSGVQNAAEVSLLTTIPYALATIAMVMTGRHSDNVLERRWHAAVPAVIGALGLV
                     LATTTAQNPMLTVAFLSVGAMGVMTAMSQFWCYPPAFLGGVAAAAGIAMINSIGNLAG
                     FISPYLIGWVKDTTGSTSIALYCIAAALVCAAVVCLAMPKSVVNR"
BASE COUNT          880 a         1064 c         1051 g          708 t
ORIGIN      
        1 atgaagggat tgccgaacgc gcagacttgg gcggtttgtc gttcgaccgt gccgcgctcg
       61 tcctctccgc cgcagcggat gggctgggta ttgccctgga gtccacccgt ctggcagaga
      121 cagagttgcg ccaaggctct ctggtcgtac tgaatggaca tcagcctccc ttgcagcggg
      181 agatgcattt cctctgctac cgtggctcga tgaaagggag ccgcaaaatt gagcggttca
      241 aggaatggat catgaaccag atccatgaag cttgatgctc gcccttcacc gctccactgt
      301 cagcaacaac ggctaaccct aggcccctgc aatacccgta ttcatccaga tcatcagccc
      361 ggttagggta gccagggaac ccagggtgga gatcaggatg gtgcgggaga taagccctgc
      421 ctcccgacga tagaattcag ccagcatgaa gggcccagta ccggtgggca aggcgctgag
      481 caacagcgcg gaattggccc acatcgactg cagagcgaag actttgaacg ccaggaacca
      541 ggtcagtagc ggctgtccca cgagcttgat ggtgaccagc ggccaagggt tgctcttccc
      601 tccagtcgac ttgaccgttt tctggcccaa gaacaggcca agcgaaacga gagcacaagg
      661 cgtggtagcc gtagcgagca tgtcgaggaa gctcatgact gggtgagcca gcggaatacc
      721 tgtcattgcc catacagccc cagcgatggg cgcaatcacc agagggtttt tggacagcgc
      781 cttcactacg acccctactg ctctgccgag atgccgctct tcctgcaagc cgatttcgat
      841 gcagatcacc gcaatggcga agagcaggca cacaacgatg atcgtcgaaa tgagcgccgg
      901 ttgaagacct ggatggccga agacaatcgc acacagcgga atgccgatgt agccagtgtt
      961 cgcatagcca gcgctcaagc cgtcgatgct tgaagtggca aggctgtggc cggtactcaa
     1021 ccgccaaatc aaagtagcga caaaaatggc cagcgtgccg gctccgaagg aaatgatgaa
     1081 gccaggctgc cagatttcag cccacgacgc ggtcgcggtc accttgaaca gcagcgcggg
     1141 taggcagagc cataccacca tcttgttgat ctcggaggcg gcctggtctc cgagtcggtt
     1201 ggtcttgcgg cagatgaagc ctgcgaggat gagggcaaag atcggcaaaa cgattgcaag
     1261 gacggtgtac atgacttcca cgtttggagg ggattttagg cctgcataga ttaaaatcgc
     1321 cgaccaatgc agtccaatga ctatgaagta tcagatcgat acctctggag catcaatgat
     1381 cgaacatcgc cacttacgct actttcttgc cttggccgac cacctgcact acggcaaagc
     1441 atcagcgcag gtaaacgtag cgcagccctc gctgagtcgg cagattgctg atcttgaatc
     1501 tcagctaggc tgccggctgt tcgctcgcac ctcccggtcg gtggagctca ccgcagcagg
     1561 ttccgagttt caacgaagcg cgcaactagt catacaggct atggactcgg cgattcgctc
     1621 cactcaagct gtcgctcggg gcgagaaggg ggagttgcgt atcgcattta cgtccatgat
     1681 cgcctggtct ggcctaccca agatgatcaa gtcctacgct gtaaacgctc ccggtgtgaa
     1741 ccttgaactg agcgagctga tacctggcgc cttgatgaaa gctgtcgaga ccggagagtg
     1801 cgatattggc ctgaccttca aagccacggt tcagccaccg tatcaatatc gggaattgca
     1861 gcgtgagtcc ctgagtatcg cggtgccggg agatcatccg ctggcacgca aaaagtcggt
     1921 aacagtccaa gacctcattg atgagccctt catcttgagc cctcgcgaca ccgcctccgc
     1981 cctgcacgac acaatcttca aactatgtcg agacagtgga ttcgaacctc gcgtgcggat
     2041 gtacacccag ctgcaaggga cgatagtcaa tctggttggt gaaggtctcg gcgtctcgat
     2101 cattccaacg tccatgtcga aatcgaatcc agactcggtt ctgttcattc caattaccaa
     2161 cgcacccgag atcaccttgg ggatggtctg gaacatggag aactccaacc catgcctctc
     2221 cgccttcatt caatcggtcg gtacccgcct cgatccgcca tgatcactcg gcgggtatcg
     2281 acatttgttt gctagcgatt caccaccgat ttgggcatcg ccaggcagac caccgcagca
     2341 cacaccagcg cagccgcgat gcagtagagc gcgatgctgg tagaaccagt ggtgtctttc
     2401 acccagccga tcaagtaggg gctgatgaac cccgccaggt tgccaatgga gttgatcatc
     2461 gcgattcctg ccgccgccgc tacaccgcct aggaatgcag gcggatagca ccagaactgg
     2521 ctcatagcag tcatcacgcc catggcaccc accgagagaa atgccaccgt caacatgggg
     2581 ttttgcgctg ttgtggtggc gagcaccaac cccaaggcgc cgatcaccgc aggcaccgcc
     2641 gcatgccagc gacgctctag tacattgtcc gaatgacgcc cggtcatgac catggcaatc
     2701 gtcgccaggg catacgggat agtggttagc agtgagactt ccgccgcatt ctgaactccc
     2761 gagtttttga tcagcgacgg aagccagaag gtgattccgt tcaagcccat catcacgccg
     2821 aagtagcctg cactcagcag ccacaccttg ggattggtga aggcgctgaa agcagagtgt
     2881 ccatggtgcg actggtcttc tccctgaaga ttacgcttta aaacggcctt ctcttctgta
     2941 gtgagccact tggcatcgtc cggattgtcc cgcaggtaga agaggacgac gaagccggcg
     3001 atgatcgaag gtattgcttc aagggcgaac agccactgcc aggctgaata gccatgccac
     3061 ccgtgagtgg tcgacatgat ccaccccgag agtggcccgc ccaggacacc cgccatgggg
     3121 atgccagtga ggaacatcgc agtaattttc ccgcgccggg aagacgggaa ccagtaggtc
     3181 aggtaaagga tgataccggg gaagaatccg gcctcagcaa cccccagcag gaatctcagc
     3241 atgtagaaca tggcgggcgt ttgtacgaac accatacagg ctgacagtac accccagctg
     3301 accatgatgc gggcgatcca ccgacgagcg ccgatcttgt gcagcatcat gttgctcggc
     3361 acctcaaaga tgaagtaacc gaagaaaaag atcccggccc caagaccgta cacggtctcg
     3421 ctgaacttga gttcgtcgag catctgcaac ttggcaaagc cgacgttcac ccgatccagg
     3481 taagcgaaca cgtagcacag aaagagaaag gggatcagtc gaagggttaa gcgcgcgtac
     3541 agctttgact cgtccggaat ggaacgcgaa tcggggacgc aggcagcatc aagagcatta
     3601 ggcatctgcc tatctccagt cattgttatt gtgcggtaag gaagaggcgc cgcccggacg
     3661 gacagcgcca gagggcgtta cgagaacatg tcaggctgct cag
//
LOCUS       DATMTW010000351         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_30772, whole genome shotgun sequence.
ACCESSION   DATMTW010000351 DATMTW010000000
VERSION     DATMTW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30772"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..864)
                     /locus_tag="VN084_06875"
     CDS             complement(70..864)
                     /locus_tag="VN084_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWT29219.1"
                     /translation="MNMYVMASERILPIAGFPVLEATPKALASHLLCILRHQQKVTLL
                     YANSNFIVKCKRLLRRMHDDSVIIVNDGVGMDIAARMFHGRSFKANLNGTDFTPYLFR
                     ESVRPLRVYLLGGRLDVVMKAAIHAEQALGQIVVGYCDGYQGMQDPSLIDRINASGAE
                     VLLVAMGNPMQEAWILEHRERLTVPIVSGVGALFDFWAGDKPRAPRYVQRLRLEWLYR
                     LCHEPRRLLRRYTVDVLVFFADCFRYRKATAPTRVMQEIDPQGKPQ"
     gene            complement(1287..>2186)
                     /locus_tag="VN084_06880"
     CDS             complement(1287..>2186)
                     /locus_tag="VN084_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWT29220.1"
                     /translation="FAPNSFWYSPIPVDAPLHPNSAGFTADFLRQKKAYYDTVNIQTS
                     DYASPIYTVGADVPTVKVQQWDCHKNGLTDPALGEQWAAVPIPAYAQPSKGTDGEMTI
                     YQPATQTVWEFWQTRKIGGQWQACWGGRLQNAQRSQGVFPGHYGTTATSLPFLGGQIT
                     ADELRRGEIRHAIGIALVETEAASIYSWPANRSDGLNPKKLPNRIPEGIRFRLDPRVD
                     VDALPMSRAGKIIAKAAQKYGFIVWDKSGAIAIRAQNSVSYTALGQPNPYPELFGGKP
                     NYSVLQGFPWDKLQFLPMHFGKP"
BASE COUNT          425 a          626 c          689 g          446 t
ORIGIN      
        1 cctttctaga gaactggata gatggaatac gcgatcgatc gcagatttga tcgatcgtaa
       61 aatcggacgt cattgcggct taccttgcgg atcgatctcc tgcatgaccc gggtcggcgc
      121 ggtggccttg cgataacgga aacagtcggc gaagaagacc agcacatcga cggtatagcg
      181 ccgaagcaaa cggcgtggct cgtggcacaa ccggtagagc cattcgagac ggagtctctg
      241 gacatagcgt ggcgccctgg gcttgtcgcc cgcccagaaa tcgaacaagg cgccgacgcc
      301 gctgacgata ggcacagtca aacgctcacg gtgctccagg atccaggctt cctgcatggg
      361 gttacccatc gccaccaaga gcacttccgc gccagaagca ttgatgcggt cgatcaggct
      421 ggggtcctgc atcccctggt agccatcgca atagccgacg acgatctggc ccagcgcctg
      481 ctcggcatgg atcgccgcct tcatcaccac gtcgagtcgc ccgcctagca gatagacgcg
      541 cagcggccga accgactcgc gaaacaggta gggcgtgaaa tcggtgccgt tgaggttggc
      601 cttgaagctg cgaccatgga acatgcgcgc ggcgatatcc atgccgacgc catcgttgac
      661 gatgatcacc gaatcatcgt gcatgcgccg caacaggcgc ttgcatttga cgatgaagtt
      721 actgttggca tagagcaagg tgactttctg ctgatgacgc aggatgcaga gcaagtggct
      781 cgccagtgct ttcggtgtcg cttccagcac ggggaatccc gcgatcggca gaatgcgttc
      841 ggacgccatg acgtacatat tcatgaagcg tctccgtgca tgtgtacctg tgatcctgtc
      901 tggcggcgca ctgactcgac gcggcggatg gtgtgacctg atggcatgtg gctccccttg
      961 tggtgcgttc ggagtactga atcgcaaaga ccatgccaga gatgtaacgc tatgttttat
     1021 atggacttat cgtcgatccg ccgccattcc gccccttttg tatgtctgcg tatgacgaca
     1081 gcaaccgcgg ggatataact aagtaaatga ttcttatcgg taaattatgt ctcttgcggc
     1141 agacgctttg cctgccctgc gtttcggtac cggcggccgc caccgcatcg cccgcgacgc
     1201 gatgcgatga ggcagtcagg gcctcgcagt cagcgcggtg ggctgaagcg cgtctgacta
     1261 aagcgcggca ggcaaagctg ggttgatcaa ggcttgccga aatgcatggg caggaactgc
     1321 agcttgtccc aggggaaacc ctgtaatacc gaatagttag gcttgccgcc gaacagctcg
     1381 ggataagggt tgggttgacc gagcgcagtg tagctgacgc tgttctgcgc gcgtatggcg
     1441 atggcgccgg atttatccca gacgatgaag ccatatttct gcgcggcttt ggcgatgatc
     1501 ttgccggcgc ggctcatggg tagcgcgtcg acatcgacgc gtggatcgag cctgaagcgg
     1561 atgccctcag ggatacggtt aggcagcttt ttcggattga ggccatccga ccgattggcc
     1621 ggccaggaat agatgctggc agcttcggtc tcgaccagcg cgatgccgat ggcatgccgg
     1681 atctcgccgc gtcgcaattc atccgccgtg atctggccgc cgaggaatgg caggctggtg
     1741 gccgtcgtgc cgtagtggcc cgggaacacc ccctggcttc gctgggcgtt ctgcaaacgg
     1801 ccaccccagc atgcctgcca ctggccgccg atcttgcggg tctgccagaa ttcccacacg
     1861 gtctgcgtcg ccggctggta gatggtcatc tcgccgtcgg tacccttgga aggctgggca
     1921 taggccggga tgggcaccgc cgcccactgc tcgccgagcg cagggtcggt gagcccgttc
     1981 ttgtgacaat cccactgctg gaccttgacg gtcggcacgt cggcgccgac ggtatagatc
     2041 gggctggcgt aatcgctggt ctggatattg accgtgtcgt aatacgcttt cttctgccgc
     2101 aggaagtccg ccgtgaaccc ggcagaattg gggtgcagtg gcgcatcgac cgggatcggg
     2161 ctgtaccaga aactattggg cgcgaa
//
LOCUS       DATMTW010000352         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_65069, whole genome shotgun sequence.
ACCESSION   DATMTW010000352 DATMTW010000000
VERSION     DATMTW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65069"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="kdsA"
                     /locus_tag="VN084_06885"
     CDS             <1..526
                     /gene="kdsA"
                     /locus_tag="VN084_06885"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWT29221.1"
                     /translation="VLQTPAFLCRQTDFITACAKSGKPVNIKKGQFLAPGDMRNVVQK
                     AKEANGGADTIMVCERGVSFGYNTLVSDMRSLAIMRETHCPVVFDATHSVQQPGGQGD
                     KSGGQREFVPVLARAAVASGIAGIFMETHPDPAKALSDGPNAWPLPKMEALLQVLIDM
                     DKLVKKAGFIEATL"
     gene            593..1879
                     /gene="eno"
                     /locus_tag="VN084_06890"
     CDS             593..1879
                     /gene="eno"
                     /locus_tag="VN084_06890"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWT29222.1"
                     /translation="MSAIVDIIAREIIDSRGNPTVEADVLLESGIIGRAAVPSGASTG
                     TKEAVELRDGDKSRYLGKGVLQAVENVNTEICEAIIGLDVEEQSFIDNTLIDLDGTEN
                     KSRLGANAILAVSMACARAAAEESGLPLYRYLGGSSFMQLPTPMMNIINGGAHAENSV
                     DMQEFMIIPAGLPSFREALRAGAEVFHALKKTLHKNGLATTVGDEGGFAPDLPSNEAA
                     IQYILEAISDAGYEPGRDILLGLDCASSEFYKDGKYHLESEGVQLTSAQFTDYLAAWV
                     DKYPIISIEDGMSEHDWDGWKLLTDRMGKNVQLVGDDLFVTNAKILREGIKRGVANSV
                     LIKVNQIGTLTETFTTIETAKRAGYTSVISHRSGETEDTTIADISVATNALQIKTGSL
                     SRSERIAKYNQLLRIEEELGDATSYAGMDAFYHLGR"
     gene            1905..2291
                     /gene="ftsB"
                     /locus_tag="VN084_06895"
     CDS             1905..2291
                     /gene="ftsB"
                     /locus_tag="VN084_06895"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HWT29223.1"
                     /translation="MRVLTLIFVILIASLQYPLWLGKGSWLRVWDLNRQITEQKDKNK
                     LLKARNDTLDAEVRDLKQGYAAIEERARSELGMIKQDEVFYQVLQAGAPTTYAPPPVD
                     PALKAAGKEAGHEAGTKSESKPAPTP"
     gene            complement(2429..>2972)
                     /locus_tag="VN084_06900"
     CDS             complement(2429..>2972)
                     /locus_tag="VN084_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HWT29224.1"
                     /translation="TLENGVILRLPGSVPRFEVFANAERATALWQTLAETHMPAGKAV
                     WDWLEIQAGIPDVYPATQEAFVPQMLNLDAIGGISFKKGCYTGQEIVARTHYLGKVKR
                     RTQIAHIGSAQPPQAGDDIVTAAGGEAAGKIVRSAAAPAGGYDVLTEVRLESLSEPLN
                     WQGHVLTFKPLPYQLPDKAA"
BASE COUNT          607 a          927 c          886 g          552 t
ORIGIN      
        1 tgtgctgcag accccggcct tcctctgccg ccagaccgat ttcatcaccg cctgcgccaa
       61 atccggcaag ccggtcaaca tcaagaaggg ccagttcctc gcacctggcg atatgcgtaa
      121 tgtcgtgcag aaagccaagg aagcgaacgg cggcgcagac accatcatgg tctgcgagcg
      181 tggcgtctcc ttcggttaca acacgctggt gtccgacatg cgcagcctcg ccatcatgcg
      241 cgagacccat tgtcctgtgg tgttcgacgc cacccattcg gtacagcaac cgggcgggca
      301 gggcgacaag agcggcggcc agcgcgagtt cgtgccggtg ctggcgcgcg cagcggtggc
      361 cagcggcatc gccggcatct tcatggaaac ccatccggac ccggccaagg ccttgtccga
      421 cggccccaac gcctggccgc taccgaagat ggaagccttg ctgcaggtgc tgatcgacat
      481 ggacaagctg gtcaaaaaag caggattcat tgaagccacg ctctaagtgg actattgcct
      541 tccggctggg aacattttca aatcttaggt ttaattcaaa agggtaggaa acatgagtgc
      601 aatcgtagac atcatcgcgc gtgagatcat cgactcccgc ggcaacccca ctgtcgaagc
      661 ggacgtgttg ctggaatccg gcatcatcgg ccgtgccgcc gtgccttctg gcgcctccac
      721 cggcaccaag gaagcggtcg aactgcgtga cggcgacaag tcccgttatc tgggcaaggg
      781 cgtgctgcag gcggtagaga acgtcaacac agagatctgc gaagccatca tcggcctcga
      841 tgtcgaggaa cagagcttca tcgacaacac cctgatcgac ctcgacggca ccgagaacaa
      901 gagccgcctg ggtgccaacg ccatcctcgc cgtttccatg gcctgcgccc gcgctgcggc
      961 cgaggagtct ggcctgccgc tgtaccgcta ccttggtggt tccagcttca tgcaattgcc
     1021 gacgccgatg atgaacatca tcaacggtgg tgcccacgcc gagaacagcg tcgacatgca
     1081 ggaattcatg atcatcccgg ccggcctgcc gagcttccgc gaagcgttgc gtgctggcgc
     1141 tgaagtgttc catgcactga agaagacgct gcacaagaac ggcctcgcca ccaccgttgg
     1201 cgacgaaggc ggtttcgcgc ctgacctgcc ttccaacgaa gcggctatcc agtacatcct
     1261 ggaagcgatc tccgacgctg gctacgagcc gggtcgcgac atcctgctgg gtctggattg
     1321 cgccagctcc gagttctaca aggacggcaa gtatcacctt gagtccgaag gtgtgcagct
     1381 cacctccgcc cagttcaccg attacctggc ggcctgggtc gacaaatacc caatcatcag
     1441 tatcgaagac ggcatgagcg aacatgactg ggacggctgg aagctgctca ccgaccgtat
     1501 gggcaagaac gtgcagctgg tgggtgacga cctgttcgtg accaacgcca agatcctgcg
     1561 cgaaggcatc aagcgtggcg tggcgaactc tgtgctgatc aaggtcaacc agatcggcac
     1621 cctgaccgag accttcacca ccatcgagac cgccaagcgc gccgggtata cctcggtcat
     1681 ctcgcatcgt tccggcgaga ccgaagacac caccatcgcc gatatctcgg tcgcgaccaa
     1741 cgcgctgcag atcaagaccg gttcgctgtc gcgctccgag cgtatcgcca agtacaacca
     1801 gctgttgcgt atcgaggaag agctcggcga cgccaccagc tacgccggta tggacgcgtt
     1861 ctaccatctg ggtcgttgag cgtctggctc actaagcgcg ttttgtgcgt gtcctgacgc
     1921 tgatcttcgt catcctcatt gcctcgctgc agtatccgct gtggctgggc aaggggagct
     1981 ggttgcgggt ctgggacctg aaccgccaga tcacggagca gaaggacaag aacaagttgc
     2041 tgaaagcgcg taacgatacg ctggatgccg aagtgcgcga cctcaagcag ggatatgccg
     2101 cgatcgaaga gcgtgcgcgc agcgagctcg gcatgatcaa gcaggatgaa gtcttttatc
     2161 aggtcttgca ggcaggggcg cccaccacct atgcgccacc cccggtcgac ccggcgctca
     2221 aggcggccgg aaaagaggct gggcatgagg ccgggaccaa atcggaaagc aagccggcac
     2281 cgacaccctg acgctgccta gtttcgcgcc gaccgctttc ggcaatcccc cgtggcgatg
     2341 tcgctacgtc tttccaattg aactgatgaa tcaacccgcc tgacagggcg tcatcgacgt
     2401 ggcgtctgca atagcgccga tttcgggttt atgccgcttt atccggcaac tgatagggca
     2461 atggcttgaa cgtcagtaca tgcccttgcc aattgagcgg ctccgacagg ctttccagcc
     2521 gtacttccgt caatacgtcg taaccaccgg caggtgccgc tgcactgcgg acgatcttgc
     2581 ctgcggcttc gccgccagcc gcggtgacga tatcgtctcc agcttgcggg ggttgtgccg
     2641 aaccgatgtg ggcgatctgc gtgcggcgtt tgaccttgcc cagataatgc gtgcgcgcca
     2701 cgatctcctg gccggtgtaa cagcctttct tgaagctgat accgccaatg gcatccaggt
     2761 tgagcatctg tggcacgaag gcttcctgcg tggccgggta gacatccggg atgccggcct
     2821 ggatctcgag ccagtcccag accgccttgc cggcgggcat gtgcgtctca gccagcgttt
     2881 gccacaaggc tgtggcacgt tcggcgttgg cgaagacctc gaagcgcggc accgatcccg
     2941 gcaggcgcaa gattacccca ttctccagag tg
//
LOCUS       DATMTW010000353         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_399315, whole genome shotgun sequence.
ACCESSION   DATMTW010000353 DATMTW010000000
VERSION     DATMTW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399315"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..490
                     /locus_tag="VN084_06905"
     CDS             89..490
                     /locus_tag="VN084_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWT29225.1"
                     /translation="MKTSEDSDGKCRLDKWLWAARFFKTRSLASDAIDGGKVHVDGDR
                     VKPAKEVRLGAVIHIRRKEFEIEVTVRALSTQRRGAPEAALLYEETPASLARRENEAI
                     TREHDHARRERGEGRPTKRQLRDIRKFTGNY"
     gene            complement(508..1566)
                     /locus_tag="VN084_06910"
     CDS             complement(508..1566)
                     /locus_tag="VN084_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWT29226.1"
                     /translation="MIFKGRGSASNPTGRFEHHQHADIDDGWGSLDADPAPLRTEVIV
                     DDTRSIINWVRSPDIPFDRSINPYRGCEHGCTYCFARPSHTYLGYSAGLDFESRLLVK
                     PNAAALLRQELAKPGYLCQPMALGTNTDPYQPIERQHQITRQVLEVLAEHRHPLSIVT
                     KSSLVERDIDLLAPMAQAGLAKVFVSITTFDRDVSRLLEPRAAAPQRRLETIRRLHDA
                     GIPVGVMTAPIIPVLTDSEMETILQQAADAGAESAGYVLLRLPLEVAPLFEEWLRLHY
                     PLKAEHVMSVVRQSRGGKANDPRFFNRMRGQGLFADMIAQRFRLATARLGLNRQRRAL
                     DTTRFNPPAVVKPQMELF"
     gene            complement(1599..>2077)
                     /locus_tag="VN084_06915"
     CDS             complement(1599..>2077)
                     /locus_tag="VN084_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWT29227.1"
                     /translation="GVPIDVLINNAGVYPMGKEAFGNVDYQAWAETFRINSMAPLKMA
                     EAFIDQLLLGKDRKIINITSKMGSIDDNTSGHHYLYRSSKTALNMVTKSLAIDLAPKG
                     IVAIVMHPGWVQTDMGGSSAPTTIPQSISGMRSVIAHLTPADAGKFYAFDGKEIPW"
BASE COUNT          389 a          596 c          650 g          442 t
ORIGIN      
        1 cgaagttctc tgcctatctg ctgggcgcga gtttcgttgc atacgccgca ttggcgctag
       61 cgtatctctg gttcagcctg cacacctcat gaaaacttcg gaagacagcg acggcaaatg
      121 ccggctcgac aagtggctat gggcggcaag attcttcaag acgcgtagcc tcgcgtcgga
      181 tgccatcgac ggcggtaagg tccatgtcga tggtgatcgt gtcaagccgg ccaaggaggt
      241 ccgtcttggt gccgtgatcc atatccggcg caaggaattc gagatcgagg tcacggtgcg
      301 cgccctgtcc acgcagcgac gcggcgcccc tgaagcggcg ctgctctatg aagagacccc
      361 ggccagtctc gcgcgtcgtg agaacgaggc catcacgcgc gagcacgacc atgcgcgccg
      421 tgaacgtggc gaagggcgtc ccaccaagcg ccagctgcgc gatatccgca aattcaccgg
      481 caattactga gacagcgcga ttccaggtca gaacaattcc atctgcggtt tcaccactgc
      541 aggcggattg aaccttgtgg tatccagcgc ccttcgctgc ctgttcaaac cgagcctggc
      601 cgtcgccagc ctaaagcgtt gcgcgatcat gtcggcaaaa agcccctggc cgcgcatgcg
      661 gttgaagaat cggggatcgt tggccttgcc cccgcggctt tgccggacca cgctcatgac
      721 atgctcggct ttgagcggat aatgtaatct cagccactcc tcgaacagcg gcgccacctc
      781 cagcggcagc ctaagcagca catagcctgc gctctcggcg ccggcatcgg cggcttgctg
      841 caggatggtc tccatctcgc tatcagtcag cactggaatg atgggcgctg tcatcacacc
      901 gaccgggatc ccggcgtcgt gcagtcgcct gatggtttcc aatcgccttt gcggcgctgc
      961 agcacggggt tccagcaggc gtgagacatc acgatcgaaa gtcgtgatgg aaacgaagac
     1021 tttggccagg ccagcctgtg ccataggcgc gaggaggtcg atgtcccgtt ccaccagcga
     1081 ggatttggtg acgatgctca aagggtgacg atgttcggcc agcacttcga gcacttgacg
     1141 ggtgatctgg tgttggcgct cgatcggctg ataggggtcg gtattggtgc ccagcgccat
     1201 gggttggcag aggtagccag gtttcgccag ctcctgcctg agcaatgcgg cggcattggg
     1261 tttgacaagt aatcggctct cgaaatcaag cccggcggag taaccgagat aagtgtggct
     1321 tggcctggcg aagcagtatg tgcagccgtg ttcgcagcca cgatagggat tgatggagcg
     1381 gtcgaaagga atgtcaggcg aacgtaccca gttgatgatg ctgcgcgtat cgtcgacgat
     1441 cacctcagtt cgtaagggcg ctgggtctgc atcgaggctg ccccagccgt cgtcgatatc
     1501 ggcatgctgg tgatgttcga aacgaccggt tgggttgctc gccgatccgc ggcccttgaa
     1561 gatcaacggc cgtagcggcg tggcgaggtt cgaccggttt accaggggat ttcttttcca
     1621 tcgaacgcat agaacttgcc tgcatcggcc ggcgtgagat gcgcgatgac gctgcgcatg
     1681 ccgctgatgc tctgcgggat ggtggtcggc gcgctactgc cgcccatgtc cgtttgtacc
     1741 cagcccgggt gcatgacgat cgccacgatg cctttgggtg cgagatcgat cgccaggctt
     1801 ttggtgacca tgttcaaggc ggttttgctt gagcggtaga ggtagtgatg gccgctggtg
     1861 ttgtcgtcga tgctgcccat cttgctggtg atgttgatga tcttccgatc cttgcctagc
     1921 aacagttgat cgatgaacgc ttctgccatc ttcaagggcg ccatgctgtt gatgcgaaac
     1981 gtctctgccc aggcctgata atcgacgttg ccgaacgctt ccttgcccat cggatagacc
     2041 ccggcgttgt tgatcagcac atcgatcgga acccctg
//
LOCUS       DATMTW010000354         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_500240, whole genome shotgun sequence.
ACCESSION   DATMTW010000354 DATMTW010000000
VERSION     DATMTW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500240"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..886
                     /locus_tag="VN084_06920"
     CDS             260..886
                     /locus_tag="VN084_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWT29228.1"
                     /translation="MTTLSDRFSQAVDYARIAHAAQVRKGSNIPYLYHLLGVASLVIE
                     FGGNEDQAIAGLLHDTIEDCGTGHEAAIRKQFGDAVANIVVDCTDGTAEGKASHTDPE
                     ARRRDWIERKLAYLAHLKQAPDATLLVSACDKLHNARAIVQDLEDPEVATRVFDRFTG
                     GRDGTLGYYQALVEIFSSRGTKVARVLDSTVDLMHALAGAGERKALAG"
     gene            973..1217
                     /locus_tag="VN084_06925"
                     /pseudo
     CDS             973..1217
                     /locus_tag="VN084_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
     gene            1243..1497
                     /locus_tag="VN084_06930"
     CDS             1243..1497
                     /locus_tag="VN084_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HWT29229.1"
                     /translation="MQLIFSDAAWEDYLHWQAHDRKLVARINKLTDDTMRHPLEGIGK
                     PEPLRHGLADYWSRRINGEHRMVYKPVADALLIAQLRYHY"
BASE COUNT          354 a          533 c          511 g          277 t
ORIGIN      
        1 agccgcaggc aggccccgca gtgcgcagcg cgaaggggtt tggcccatgg atgggcccaa
       61 caatccccgc ggacggcacg caacagcgca atgtaatgac ccagtgattt tctgctcagg
      121 tgccaacttt ttacggagag cacgatgagc caagtgatgg aagaagagat caagcgttga
      181 acggcgtccg ctacgctctc agcccttgcg acgcgccgcc atcatcattg gctacgatga
      241 aaccgtcaca gggggcagca tgaccacgct atccgatcgc ttcagccagg ccgttgacta
      301 cgcccgcatc gcccacgccg cgcaggtgcg caagggctcg aacatcccgt acctctatca
      361 cctgctcggc gtggccagcc tggtgatcga gttcggcggt aacgaagacc aggccatcgc
      421 cggcctgctg cacgacacca tcgaggattg cggtaccgga catgaggcgg cgatccgcaa
      481 gcaattcggt gatgccgtcg ccaacatcgt ggtggactgc accgatggca ccgccgaagg
      541 caaggccagc cacaccgatc cggaagccag acgccgcgac tggatcgagc gcaaactcgc
      601 ctacctcgca cacctgaaac aagccccgga cgccacgctg cttgtctccg cctgcgacaa
      661 gctgcataac gcccgcgcca tcgtccagga cctggaagat cccgaagttg ccacgcgcgt
      721 attcgatcgg ttcaccggcg gacgcgacgg gaccttgggc tactaccagg cgctggtaga
      781 aatcttctcg agtcgtggca cgaaggtcgc cagggtgctc gacagcacgg tggacctgat
      841 gcatgcgctg gcaggcgcgg gggaacgcaa ggcgcttgct ggctgattcg ccggcttgga
      901 cccgcggcat ttgaccggct gcgggccgtc ggctagatca tgtacagaat aatgtacata
      961 tcgcgatagc ccatggacac catcacctac accaaggccc gttccagcct cgccgagacg
     1021 atggagcgcg tctgcgagga tcatgcgccg gtgatcatta ccaagaagga cgagtggtga
     1081 tgatgtcgct ggccgattgc cagtcgttgg atgagaccgc gtacctgctg cgtagcccca
     1141 ggaacgcgcg ccgcctgctg gaatcgatgg cgcaactgga atccggtggc ggcaaaccac
     1201 tcaggctggc ccgctgaacc taactcgctg agcttggccg gcatgcagct gatcttctcc
     1261 gacgcggcct gggaggatta cctccactgg caggcccacg accgcaagct ggtggcgcgc
     1321 atcaacaagc tgaccgacga caccatgcgc caccccctcg aaggcatcgg caaacccgaa
     1381 cccttgcgac acgggctggc cgattattgg tcgcgccgca tcaacggcga gcaccgcatg
     1441 gtgtacaagc ccgttgccga cgcgctgctg attgcgcagt tgcggtatca ctattgagca
     1501 acaggcaacg gtcgcggctg gctttgaaaa accacccacg cggcggtgta aagacatcga
     1561 catgcgccgt cgactggtcg gggtcgatcc aacgccctat gctctggcgt gttcgagtcg
     1621 ggggagtcgc cagagtcatg cttcatggat aagaaaaaac tgtccgaagc ggaca
//
LOCUS       DATMTW010000355         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_232598, whole genome shotgun sequence.
ACCESSION   DATMTW010000355 DATMTW010000000
VERSION     DATMTW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232598"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VN084_06935"
     CDS             <1..568
                     /locus_tag="VN084_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWT29230.1"
                     /translation="IASLADDVLFVTFNGLSKNYRTCGYRAGWLVVSGEKRHATDYIE
                     GLNMLASMRLCANVPGQLAIQTALGGYQSINDLVAPSGRLCKQRDLAYKLLTEIPGVT
                     CTKPAAAMYLFPKLDPKVYPIKDDQQFILDLLLEEKVLLVQGSGFNWKTPDHFRVVFL
                     PNVDDLTEAIGRIARFLKAYRMKHGAKA"
     gene            603..1913
                     /locus_tag="VN084_06940"
     CDS             603..1913
                     /locus_tag="VN084_06940"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWT29231.1"
                     /translation="MTIKVGLLGIGTVGGGTYTVLTRNQQEIQRRTGKRIEIVKVADR
                     NLELAKKVTEGKVEVTDDAFSVVNDPNVDVVVELIGGYGIAKDLVLKAIENGKHVVTA
                     NKALIALHGSEIFAAAQKQGVMVAFEAAVAGGIPIIKALREGLAANRIEWIAGIINGT
                     TNFILTEMREKGLAFADVLGEAQRLGYAEADPTFDVEGIDAAHKLTIMSAIGFGIPTQ
                     FDKAYTEGITKLESKDIRYAEELGYRVKLLGITKRTAAGVELRVHPTLIPEKRLVANV
                     NGAMNAVVVKGDAVGPTLYYGAGAGAEPTASAVVADLIDVARLATASADQRVPYLGFQ
                     PDQMADLPILPISEIDSAYYLRMRMLDKPGVLADVTKILGDRNISIDAMMQKEPEENE
                     TEADIVILTHVTKEKNMDAAIVAIEALAAIDGKVTRIRMEELSK"
BASE COUNT          461 a          619 c          639 g          375 t
ORIGIN      
        1 gatcgcctcg ctggcggacg acgtgctgtt cgtcactttc aacggcctct cgaagaacta
       61 ccgtacctgc ggctaccgcg ccggctggct ggtggtgtct ggcgagaagc ggcacgccac
      121 cgattacatc gaaggcctta acatgctggc ttccatgcgt ttgtgcgcca acgtgccggg
      181 gcagttggcg atccagaccg cgcttggcgg ttaccagagc atcaacgacc tggtcgcgcc
      241 gagcggacgc ctgtgcaagc agcgcgacct cgcttacaaa ttgctgaccg agatcccggg
      301 cgtgacctgt accaagccgg cagcggccat gtacctgttc cccaagcttg atcccaaggt
      361 ctatccgatc aaggacgacc agcagttcat cctcgacctg ttgctggaag agaaggtcct
      421 gctggtgcag ggcagcggct tcaactggaa gacgcctgac catttccgcg tggtgttcct
      481 gccgaatgtg gacgacctga cggaagccat cggccgtatc gcccgcttcc tcaaggcgta
      541 tcgcatgaag catggcgcca aggcttaagt cgtaacgaat cagaaaagtg ggtaatcaag
      601 gcatgacaat caaagtaggc ttgctgggta tcggcaccgt aggtggcggc acatacaccg
      661 tgctgacacg caaccagcag gagatccagc gccgcaccgg caagcgcatc gagatcgtca
      721 aggtcgccga ccgtaatctc gaactggcga agaaagtgac cgaaggcaag gtcgaggtca
      781 ctgatgacgc gttcagcgtg gtcaacgatc cgaacgtcga tgtcgtcgtc gagttgatcg
      841 gcggttacgg catcgccaag gatctggtgc tgaaagccat cgagaacggc aagcatgtgg
      901 tcaccgccaa caaggcgctg atcgcgctgc atggcagcga gatcttcgcc gccgcgcaga
      961 agcagggcgt catggttgcc ttcgaggcgg ccgtcgcggg tggtatcccc atcatcaagg
     1021 cgctgcgtga gggcctcgcg gccaatcgca tcgaatggat cgccggcatc atcaacggca
     1081 ctaccaattt catcctcacc gagatgcgcg agaagggtct ggcctttgcc gacgtgctcg
     1141 gtgaagcaca gcgcctgggt tatgccgagg cagacccgac gttcgacgtc gaaggcatcg
     1201 acgccgcgca caagctcacc atcatgtcgg cgatcgggtt cggtatcccg acgcagttcg
     1261 acaaggccta caccgagggc atcaccaaac tggagagcaa ggacatccgc tatgccgagg
     1321 agctcggcta ccgcgtgaaa ctgttgggca tcaccaagcg caccgcggct ggtgtcgagt
     1381 tacgcgtaca cccgaccctg atcccggaga agcgtctggt cgccaacgtc aacggcgcca
     1441 tgaacgccgt ggtggtgaag ggcgatgctg tcggcccaac cttgtattac ggcgcaggtg
     1501 ccggtgccga accgaccgcc agtgcagtcg ttgccgatct gatcgatgtc gcccgcctgg
     1561 ccacggcttc cgccgaccag cgcgtgcctt acctgggctt ccagccggat cagatggctg
     1621 atctacctat cctgccgatc agcgagatcg acagcgccta ttacctgcgc atgcgcatgc
     1681 tggacaagcc gggtgtattg gcggacgtca ccaagatcct cggcgaccgc aacatctcca
     1741 tcgatgccat gatgcagaag gagccggagg agaacgagac cgaggccgat atcgtcatcc
     1801 tcacgcatgt gaccaaggaa aagaacatgg atgccgccat tgtcgcgatc gaggcgctgg
     1861 ccgccatcga cggcaaggtg acgcgtatcc gtatggaaga gttaagtaag tagatcgaga
     1921 aaattcgctt tgcgggatga agcgcaagga gccgcactgc gaatgacgat gacatatgtg
     1981 ttgcataggc gaggaataag cgagcacgcg acgctgcgat tcgcccgcaa agaggatttg
     2041 aatgaaatac attagtaccc gcgggcaatc gcccgccatc accttcagtg agat
//
LOCUS       DATMTW010000356         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_298297, whole genome shotgun sequence.
ACCESSION   DATMTW010000356 DATMTW010000000
VERSION     DATMTW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298297"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VN084_06945"
     CDS             <1..397
                     /locus_tag="VN084_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWT29232.1"
                     /translation="SIQAKGRRIYDITPQVLDWANRSGLQTGMLTLYIQHTSASLMIN
                     ENYDPEVLIDMERFFARLVPDGDPLFIHTAEGPDDMPAHVRTALTQTSLSIPLLDGKV
                     ALGTWQGIFLYEHRLASHTRKVLLHLIGE"
     gene            422..1612
                     /locus_tag="VN084_06950"
     CDS             422..1612
                     /locus_tag="VN084_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWT29233.1"
                     /translation="MHISPFTLSGFPKVVFGDRTLGQVPQLASEFGRRALVMTGARSF
                     IETPAWPIFRAQLQDAGLHVEHAVVDGEPSPELADQIAAQFRNAGIEVVIGIGGGSVL
                     DAAKAVAGLLRIANSVMDFLEGVGPELPYPGPEVPFIAIPTTAGTGSEATKNAVLSKQ
                     GADGFKKSFRHERLVPKYAVIDPQLLATCPKPLIAANGMDAFTQLLESYVSTRANPVT
                     DALAMSGIRAVRDSLPGWYHDEGDVAQHRANMAYAALLSGITLAQVGLGSVHGLAAPL
                     GAFFPIPHGVVCGTLVAECTRVNIALLQQRQPNSDALWRYAHVGKVLQRDMSLSERDG
                     LQALIAVLERWTEELALPKLSAYGMRETDIPQVVANCRGSSMKTNPIVLTDDEVAAIL
                     HARC"
     gene            1606..2217
                     /locus_tag="VN084_06955"
     CDS             1606..2217
                     /locus_tag="VN084_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HWT29234.1"
                     /translation="MLSERRYHGVLLATLAAVFIWSLVRPHDGFTWLLEAFPVIIGLP
                     ILLLTYRRFPFSRLAYGLLLLHAVILLVGAHYTYAEMPLFNWLRDSYDLDRNYYDRLG
                     HLAQGFIPAIVVREILLRQSPLKPGKWLAFVVLSICLAISALYEMLEWWVAVASGSDA
                     VAFLATQGDVWDTQWDMFLCLLGAIASLTLLSHRHDRSLTRLH"
     gene            2296..3609
                     /locus_tag="VN084_06960"
     CDS             2296..3609
                     /locus_tag="VN084_06960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWT29235.1"
                     /translation="MDGHNTPPVAGKLRLVLVTETWPPEINGVAMTLSRLIKGLAQRH
                     WQVTLVRPRQRGEGRLAADVAIDHMLVPGFPIPGYSALRIGAPMFEKLRHAWKYDRPH
                     VVHIATEGPLGWAALKAARSLGIPVSSSFHTNFHRYCRHYRMGWLVGLVSRHLRQFHN
                     QCALTMVPNTALGRLLEADRYRNVVILGRGVDTALFSPMRRDETLREDWGLAPADIGV
                     LYVGRIAAEKNLELVVRTFDAIRQVMPTARMIWVGDGPESKRLRKRHPDHVFCGSQVD
                     EALAAHYASADLFLFPSMTETFGNVVPEAMASGLAVVAYDDAAASLLIEHGSNGELVA
                     LGDAAGFMDTAVRIARERDYLQRLRAEAPRISQAHSWGRVCDEFDRYLRLAMAGNSTL
                     HPAPASGAAGLDADGTDLTDVRLSREHLEDTVLLEGAHTAGDGRR"
     gene            complement(3502..5988)
                     /locus_tag="VN084_06965"
     CDS             complement(3502..5988)
                     /locus_tag="VN084_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWT29236.1"
                     /translation="MNIDRLWGVMEEYMDTLLEWPTPPYFKDATAGAADQAETCVLYM
                     RDGRKILGDLLQFSPEAAQLVFLPTRDEASETIALDRIKSVRLVRSLMLKPQSIALEK
                     RTEEIHPPSERQQYTVEFKDKETLRGETLGYANTANGLYLFTPGEDQKIIRSFIPHLA
                     MEQFQIGPFIGEMLVEDKLASKAEVDAALDRQRELRVQRIGDYLSEHQIVSRDQLSLA
                     IKHQESQPILKLGEALLQLGLLSEAQLEDALTKQKENRKIQLGQILVGMGVIDERTLK
                     GALAKKLGIPYVSLGKFNFDPNALRLTNASFARRHLLIPLYLHGDRLVVAFENPLDTK
                     AIEELHFMAQVKTIPVMASRDDILSAIGRNYEASSTLHFPSEAAVSASGKDEYAFHQN
                     SDVKIDDLANKLFDEGIELELVEKAIPESDNTLVQLVNKIILDAYHEGVSDIHIETYA
                     GRQNTRVRFRKDGALTPYLEIPPNFRNAVISRIKVMSNLDISERRKPQDGKLDFQHFG
                     PAKIELRVATIPTSSGLEDIVMRILAAAKPVPIDGLGLAEDALETLKRLMLRPYGLIL
                     VCGPTGSGKTTTLHSLLGYINTPERKIWTAEDPIEIAQPGLRQVQVNSKIGWTFANAM
                     RSFLRGDPDVIMVGEMRDQETTKVGIEASLTGHLVLSTLHTNTAPESVVRLLDLGMDP
                     FNFSDALLAILAQRLARALCPECKTAHEASTAEIEALVAEYCEGTPLDAGEALQAWKA
                     TYSKNGKFTLYQPNGCKHCNHTGYRGRMGLHELMVITPEVKKRIQTRAPVSELLPAAI
                     AGGMRTLKQDGILKVLAGKTDISQIRAVCI"
     gene            complement(6074..>7087)
                     /locus_tag="VN084_06970"
     CDS             complement(6074..>7087)
                     /locus_tag="VN084_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWT29237.1"
                     /translation="ISETNRKLYELSKQHKQNRNTLASLQQQKLETEGTVKEQQDLLG
                     AQLYQQYLHGQQGYLQILLQQQDPGAIARQLHYYSYVAKARATLIASMQQNLGKIAKL
                     NDETASTLKTLTELKQQQEKERRELQAQKNERNKVLKQLSAKITSQRGQISKLKRDEK
                     RLSQLVERLSRIIPKTLPKNRGKSAKASEPVARNETLPTDAFDGGDFAALKGKLNLPV
                     RGDITNRFGDSREDTGVSWKGLFIKSSEGSEVKSIASGRVVFADWLRGFGNLLIVDHG
                     DGYMSLYGNNQTLLKQVGDTVSGGDTIAAVGNSGGNETDGLYFELRYRSKPLDPLSWS
                     RLR"
BASE COUNT         1186 a         2147 c         2240 g         1514 t
ORIGIN      
        1 cagcatccag gccaaaggcc gccgcatcta cgacatcacg ccgcaggtac tcgactgggc
       61 caacaggagc ggcctgcaga ccggcatgct gacgctctac atccagcata cctcggccag
      121 cctgatgatc aacgagaatt acgatcccga agtgctgatc gacatggagc gcttcttcgc
      181 gcggctggtg ccggacggcg accccttgtt catccatacc gccgaaggtc cggacgacat
      241 gccggcgcat gtgcgtacgg cgctgaccca gaccagcctt tccatccctt tgctcgacgg
      301 caaggtggcg cttggcacct ggcagggcat cttcctctat gaacatcgcc tggcatcgca
      361 tacgcgcaag gtgctgttgc acctgatcgg cgaataagcc gccgctgtat ccggtagcgt
      421 catgcatatc tcacctttta cgctcagcgg tttccccaag gtcgtcttcg gcgaccgcac
      481 gctggggcag gttccccagc ttgcatcgga gttcggccgc cgcgcgctgg tgatgacggg
      541 cgcgcgctcc ttcatcgaga cgccagcctg gcccatattt cgcgcccagc tccaagatgc
      601 cggattgcat gtcgaacatg ccgtcgtcga cggcgagcct tcgccggaac tggcggatca
      661 gatagcggca caattccgta acgcagggat cgaggtcgtc atcggcatcg gcggtggtag
      721 cgtgctcgac gcggcgaaag ccgtggccgg cctgctgcgc atcgccaaca gcgtgatgga
      781 tttcctcgag ggcgtggggc cggagttgcc gtatcccgga ccggaagtgc cgttcatcgc
      841 gatccccact acggcaggca ccggctccga ggcgaccaag aacgcagtgc tttcgaagca
      901 gggcgctgac ggtttcaaga aatcctttcg ccatgaacgg ctggtgccga agtatgcggt
      961 gatcgatccg caattgctcg cgacctgccc taaaccactg attgccgcca acggcatgga
     1021 tgccttcaca caattgctcg aatcctatgt ctccacgcgt gccaacccgg tgactgatgc
     1081 gttggcgatg tccggcatcc gcgctgtgcg cgacagtctg ccgggctggt atcacgatga
     1141 gggcgatgtg gctcagcacc gtgccaacat ggcgtatgcc gcgttgttgt caggaatcac
     1201 gctggcccag gtggggctgg gatctgtgca tgggctggcg gcgccgctgg gcgccttttt
     1261 cccgatcccg catggggtgg tctgcggcac gctggtggcc gagtgcaccc gggtcaacat
     1321 cgccctgctg cagcaacgcc agccgaatag cgacgcgctc tggcgctacg cccatgtcgg
     1381 caaggtgctg cagcgtgaca tgagcctgag cgagcgcgac gggctccagg ccctgatcgc
     1441 cgtactcgag cgatggacgg aagaactggc gctgcccaag ctctccgctt acggcatgcg
     1501 cgagaccgat atcccgcaag tggtggcgaa ttgccgcggc agcagcatga agaccaaccc
     1561 catcgtgctg accgacgacg aggtcgccgc gatcctgcac gcgcgatgct gagcgaacgc
     1621 cgttaccatg gggttctgct ggcgacgctc gcggccgtct tcatctggtc attggtgcgg
     1681 ccgcatgacg gttttacctg gctgctggaa gccttcccgg tcatcatcgg attgccgatc
     1741 ctgctgctga cctatcggcg tttcccgttt tcgcgcctgg cctatggcct attattgctg
     1801 cacgccgtca tcctgctggt cggggcacat tacacctacg ccgagatgcc gctgttcaac
     1861 tggttgcgcg atagctacga cctcgatcgc aattattacg accggcttgg ccatcttgcg
     1921 cagggcttca tcccggccat cgtcgtgcgg gagatcctgc tgcgccagtc gccgctgaag
     1981 ccgggcaagt ggctggcgtt cgtcgtgctc agtatctgcc ttgccatcag cgcgctctac
     2041 gagatgctgg agtggtgggt ggcggtggcg tccggcagcg acgccgtggc gttcctcgcg
     2101 acgcagggcg acgtctggga tacccagtgg gacatgttcc tctgcctgct cggggcgatc
     2161 gcgtcgctga cgctgctctc gcatcggcat gatcgctcac tgacgcgact gcactgacac
     2221 gaccgcactg acactgtcac cccggtgtca cgctgatgtc atggactggt cacgccccgg
     2281 cgccagaatc cagccatgga tggacacaat accccgcctg tagccggcaa gttgcgtctg
     2341 gtactggtca ctgaaacctg gccacccgag atcaacggtg tggccatgac cctgtcccga
     2401 ttgatcaagg gcttggcgca gcgacactgg caggtgacgc tggtgaggcc gcgacagcgt
     2461 ggcgaaggca ggctcgctgc tgatgtcgcg atcgatcaca tgctggtgcc cggcttcccc
     2521 atccccggtt acagcgcgtt gcgcatcggc gcgcccatgt tcgagaaact gcgccatgca
     2581 tggaagtacg accggccaca tgtcgtgcat atcgccaccg aggggccgct gggctgggct
     2641 gcgctgaaag cggcgcgtag cctcggcatc ccggtcagca gcagcttcca taccaatttc
     2701 caccgctatt gccgccacta ccgcatgggc tggcttgttg ggctggtgag ccgccacctg
     2761 cggcaattcc acaaccaatg tgccttgacc atggtgccta atacggcgct cggaaggttg
     2821 ctggaggcgg atcgctatcg caatgtggtg atcctcggac gcggcgtgga tactgccctg
     2881 ttttcgccca tgcgccgcga cgagaccttg cgcgaagact ggggcttggc gcctgccgac
     2941 atcggggtgc tgtatgtcgg ccgtatcgcc gctgagaaga acctggagct ggtggttcgg
     3001 acattcgacg ccatccggca ggttatgccg acagcccgca tgatctgggt cggcgacggc
     3061 ccggaatcga aacggctgcg caaacggcat ccggaccatg tcttctgcgg cagccaggtg
     3121 gacgaagccc tggccgcaca ctatgcctct gccgacctgt tcctgttccc cagcatgacc
     3181 gagactttcg gtaacgtcgt gccggaagcc atggcgagcg gcctcgcagt cgtggcctat
     3241 gacgatgcgg cggcttcgct attgatcgag catggcagca acggcgaact cgttgccctg
     3301 ggggatgccg cgggcttcat ggatactgct gtgcggatcg cgcgcgaacg cgactatctg
     3361 cagcgattgc gcgccgaagc gccgcgcatc tcgcaggcgc attcatgggg gcgggtctgc
     3421 gacgaattcg accgttacct gcggctagcc atggccggta atagcacatt gcatccggcg
     3481 ccggcgagtg gagctgcggg tctagatgca gacggcacgg atctgactga tgtccgtctt
     3541 tcccgcgagc accttgagga taccgtcctg cttgagggtg cgcataccgc cggcgatggc
     3601 cgcaggtaat agttcgctga ctggcgcccg cgtctggatg cgctttttca cttcaggggt
     3661 gatgaccatc aactcatgta gccccatgcg ccctcgataa ccggtatggt tgcaatgttt
     3721 gcaaccattc ggctggtaga gggtgaactt gccgttcttg ctgtaagtgg ccttccacgc
     3781 ctgcagcgct tcgccggcat ccagcggggt gccttcacaa tattcggcga ccagcgcctc
     3841 gatctccgcc gtgctcgctt catgggcagt cttgcattcc gggcacaacg cgcgtgccag
     3901 ccgctgggcg aggatcgcca gtaaggcatc ggagaaattg aacggatcca tccccaggtc
     3961 gagcaacctg accacactct cgggtgcggt gttggtatgc agtgtggaga gcaccagatg
     4021 gccggtgagc gaggcttcga tgccgacctt ggtggtctcc tggtcgcgca tctcaccgac
     4081 catgatgaca tcggggtcgc cacgcaggaa actgcgcatg gcgttggcga aggtccagcc
     4141 gatcttgctg ttgacttgca cctgacgcag gcccggctgg gcgatctcga tcggatcctc
     4201 agccgtccag atcttgcgtt ccggcgtgtt gatatacccc agcagggagt gcaaggttgt
     4261 cgtcttgccg gacccggtcg ggccgcagac caggatgagg ccataaggcc ggagcatgag
     4321 tcgtttcaat gtctccagcg cgtcttccgc cagccccagc ccgtcgatcg gtaccggttt
     4381 ggccgccgcc aggatgcgca tgacgatatc ctccagcccg ctgctggtgg ggatggtcgc
     4441 gacacgcagt tcgatcttcg ccggaccgaa atgctggaaa tcgagcttgc cgtcctgtgg
     4501 tttgcgccgt tcggagatgt cgagattgga catcaccttg atgcgcgaga tgacggcatt
     4561 gcggaagttg ggcgggatct ccagatatgg cgtgagcgcg ccgtccttgc ggaaacggac
     4621 ccgcgtgttc tgcctgccgg cgtaggtttc gatatggatg tcggaaacgc cttcatggta
     4681 agcatcgagg atgatcttgt tgaccagttg cacgagggtg ttgtcagact ccgggatggc
     4741 tttctcgacc agttcgagtt cgatcccctc atcgaagagc ttgttggcga ggtcgtcgat
     4801 cttgacgtcg ctgttctggt gaaaggcgta ctcatccttg cctgaggcgc tcacagcggc
     4861 ctcggaaggg aaatgcaagg tggaagaggc ctcatagttg cggccgatcg cactaaggat
     4921 gtcatcgcgc gacgccatca ccgggatggt cttgacctgc gccatgaaat gcagctcctc
     4981 gatcgccttg gtgtcgagcg ggttctcgaa ggcgaccacc agccggtcgc catgaaggta
     5041 aagcggtatc agcagatgcc gcctggcgaa actggcgttg gtcagacgta gcgcgttggg
     5101 gtcgaagttg aacttgccca gactgacata agggatgccg agtttcttcg ccagcgcacc
     5161 cttgagcgta cgttcgtcga tcacgcccat gccgaccagg atctggccga gttgtatctt
     5221 gcggttctct ttctgcttgg tcagggcgtc ctcgagttgc gcttcgctca acagccccag
     5281 ttgcagcagc gcttcgccga gtttgaggat gggctggctc tcctggtgct tgatcgcgag
     5341 actgagctga tcgcgcgaga cgatctggtg ttccgagagg taatcgccga tgcgctgcac
     5401 ccgcagttcg cgctggcgat cgagcgcggc gtccacctcg gccttgctcg ccagcttgtc
     5461 ttcgaccagc atctcgccga tgaaagggcc gatctggaac tgttccatgg ccaggtgcgg
     5521 gatgaagctt cggatgatct tctggtcttc gcccggggtg aacaggtaaa gaccgttggc
     5581 ggtattggca taccccaggg tctcgccccg cagggtctcc ttgtccttga actccaccgt
     5641 gtattgctgg cgctccgaag gtggatggat ctcttccgtg cgtttttcca gtgcgatgga
     5701 ctggggtttc agcatcagcg atctgaccag gcggacgctc ttgatgcggt cgagcgcgat
     5761 ggtttcgctg gcttcatcgc gggtcggcag gaaaacgagc tgcgcggcct ctggcgagaa
     5821 ctgcagcaga tcacccagta tcttgcggcc gtcgcgcatg tacagcacgc aggtctccgc
     5881 ttgatcggcg gcgccggcgg tcgcgtcctt gaagtagggc ggtgtcggcc attcgagcag
     5941 ggtatccatg tactcctcca tcactcccca taggcgatcg atgttcatgg gctatctctt
     6001 gtctttgccg cgatcgggat acgcgaccgg atagtcccgg taggcactgc aaacgccgct
     6061 tggcgttacg gcattatctt aatctgctcc agctcagcgg gtcgagcggt ttgctgcggt
     6121 agcgcagctc gaaatagagg ccatcggttt cgttgccgcc actgttgccg accgccgcga
     6181 tcgtatcgcc gccgctgacc gtgtcgccga cttgtttgag caaggtctgg ttgttgccgt
     6241 aaaggctcat gtagccatcg ccgtgatcga cgatcagcag gttgccgaaa ccgcgcagcc
     6301 agtcggcgaa gacgacgcga ccgctggcga ttgatttgac ctcgctgcct tcactcgatt
     6361 tgatgaacag gcctttccag gagacgccgg tatcctcgcg tgaatcgccg aaccggttgg
     6421 tgatgtcgcc gcgcaccggc agattgagct tgcctttgag cgccgcgaaa tcgccaccgt
     6481 cgaaggcatc ggtcggcaag gtctcgttgc gggccacggg ttcgctggcc ttggccgatt
     6541 ttccgcgatt tttcggcaag gtcttgggga tgatgcgcga cagccgctcg accaattgcg
     6601 acaagcgctt ctcgtcgcgc ttgagtttgc tgatctggcc gcgctgtgag gtgatcttgg
     6661 ccgagagctg cttcagcacc ttgttgcgct cattcttctg cgcttgcagt tcgcggcgct
     6721 ctttctcctg ctgctgcttg agttctgtca gggtcttgag ggtgctggcg gtctcgtcat
     6781 tgagcttggc gatcttgccg aggttctgct gcatgctggc gatcagtgtc gcgcgggcct
     6841 tggcgacata actgtaatag tgcagctggc gggcgatggc tcccggatcc tgctgttgca
     6901 gcaagatctg cagatagccc tgctggccat gcaggtactg ctggtaaagc tgggcgccaa
     6961 gcagatcctg ttgctccttg accgtgccct cggtctcgag tttctgctgc tgcaggctgg
     7021 ccagcgtgtt acggttctgc ttgtgctgct tgctcaactc gtagagcttg cggttggtct
     7081 cgctgat
//
LOCUS       DATMTW010000357         7965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_98307, whole genome shotgun sequence.
ACCESSION   DATMTW010000357 DATMTW010000000
VERSION     DATMTW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98307"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..354)
                     /locus_tag="VN084_06975"
     CDS             complement(70..354)
                     /locus_tag="VN084_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HWT29238.1"
                     /translation="MTKSELIAKLAARFPQLVVKDAELSVKAILDSMSDNLATGERIE
                     IRGFGSFSLNYRPPRLGRNPKTGTKVQVPEKYVPHFKAGKELRERVDLSQ"
     gene            complement(367..2079)
                     /gene="rpsA"
                     /locus_tag="VN084_06980"
     CDS             complement(367..2079)
                     /gene="rpsA"
                     /locus_tag="VN084_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWT29239.1"
                     /translation="MANVSTNTSSTESFAALFEESLARQEMRSGEVITAEVVSIDDDF
                     IIVNAGLKSESIIATSEFRNDRGELEVAVGDFVKVAIESLEDGYGSTKLSREKAKRLA
                     AWLDLEEAMNEGRIIKGMVNGRVKGGLTVMVNGIRAFLPGSLVDLRPVKDTTPFENKE
                     WDFKVIKLDRKRNNVVVSRRAVLEESMGADREALLANLKEGAVVKGIVKNITDYGAFV
                     DLGGIDGLLHITDLAWRRVKHPSEVLTVGEEVEAKILKFDQEKNRVSLGIKQLGDDPW
                     VALSRRYPVSTRLFGKVTNLTDYGAFVEIEPGIEGLVHVSEMDWTNKNVHPAKIAQLG
                     DEVEVMILEIDEDRRRLSLGMKQCQANPWDDFAASHKKGDKVSGQIKSITDFGVFIGL
                     PGGIDGLVHLSDLSWNQSGEEAISNFKKGDEVTAVVLAIDVEKERISLGVKQLDSDPF
                     NAYTSLNDKGAIVKGTVKSVDAKGAVVTLDGEVEGYLRASDISKEKVEDATSVLKAGE
                     EIEARITNIDRKNRNINLSIKAKDIAEEADAMQKFSNDNAGAAGTTNLGALLKAKLDG
                     KNNE"
     gene            complement(2187..2861)
                     /gene="cmk"
                     /locus_tag="VN084_06985"
     CDS             complement(2187..2861)
                     /gene="cmk"
                     /locus_tag="VN084_06985"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWT29240.1"
                     /translation="MNNDTSIPVIAIDGPSASGKGTVAQIVADRLGYHYLDSGALYRI
                     VALAARRQGLAWQDEPALAAMALKLEIRFDQGRIWLGNEDISEAVRTEEMSRGASEVA
                     VHPAVRAALLDLQHSFRKAPGLVADGRDMATVVFPDAGTKIFLTASAEVRAERRYKQL
                     MSKGNHANLADILKDLQARDARDRQRSVAPLQQSRDAELLETSALSIEQAVDSVLAHH
                     LKPRAK"
     gene            2968..3174
                     /locus_tag="VN084_06990"
     CDS             2968..3174
                     /locus_tag="VN084_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HWT29241.1"
                     /translation="MTKTLTFAAVHFSVAFTVGYALTGSVTVGGAIALVEPACNTVAY
                     FFHEKIWQRIEQGEHGSHALFHAH"
     gene            complement(3304..4596)
                     /gene="aroA"
                     /locus_tag="VN084_06995"
     CDS             complement(3304..4596)
                     /gene="aroA"
                     /locus_tag="VN084_06995"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWT29242.1"
                     /translation="METLDLSPLTRACGSIRLPGSKSISNRTLLLAALAQGTTQIHDL
                     LASDDVDRMLDALKALGVALSQTGEHDWQVEGTGGTFPVKSADLFMGNAGTAIRPLTA
                     ALAFSDGSYKLSGVPRMHERPIGDLVEGLRQAGAQVTYLGNAGYPPLQIEPAAIDVTQ
                     AIRIRGDVSSQFLTALLMALPLTGKQATIEVVGELISKPYIEITLNLMARFGVVVARD
                     GWQRFTIPAASRYRSPGKVYVEGDASSASYFLAAGAIAGGPVRVEGIGKDSIQGDVRF
                     TEALSAMGANIAWGDNWIEASAPASGKLRGVDLDCNHIPDAAMTLATAALFADGPTRL
                     RNIASWRVKETDRLSAMATELRKVGATVEEGSDYLLITPPAQLTPNAKIDTYDDHRMA
                     MCFSLVALGHTPVTINDPGCVAKTFPDYFTRFAAVTQA"
     gene            complement(4614..5525)
                     /locus_tag="VN084_07000"
     CDS             complement(4614..5525)
                     /locus_tag="VN084_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWT29243.1"
                     /translation="MNEPQSNLALHTRKLAIFGVGLIGGSLALALKRHHAIGQIVGVG
                     RQSDNMQAALDLGVIDAVAHDAADALTGADIVMIAAPVAQTPDILRKIAPHLGANTVV
                     TDAGSTKSDVQRCAQDILGARNKRFVPGHPIAGAEKSGVTAARADLYDGKNIVLTPGA
                     ETDADAVALVRTLWTAAGAHVTTMSAAEHDGIFAAVSHLPHLLAFALVDELAARDNAA
                     QLFDFAASGFRDFSRIAGSSPEMWRDISLANRPALLDELDAYAAELQKIRTLLQAADG
                     QGLQALFERASQARNAWAHKRLSPNKD"
     gene            complement(5829..6578)
                     /locus_tag="VN084_07005"
     CDS             complement(5829..6578)
                     /locus_tag="VN084_07005"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWT29244.1"
                     /translation="MTLPNDPPLQRLQFYATTPYPCGYLEGKLAQSLIAAPHHLIDAE
                     AYSGLIQLGFRRSGKFAYRPHCEHCNACIPVRLPVDRFTPNRSQRRALKQHLDLRVTV
                     SELRYSEEHFALYTAYQTARHHNGDDAESAEQYRNFLVQSNVESVMVEFRDNDALRIV
                     SVVDIVRDGLSAVYTFYDASDRSNSFGTFNVLWLIEWCRSLGLPYLYLGYWIDQSRKM
                     HYKKHFLPQEGLIDGEWQPLHHHLHPDSAAG"
     gene            complement(6585..7262)
                     /gene="aat"
                     /locus_tag="VN084_07010"
     CDS             complement(6585..7262)
                     /gene="aat"
                     /locus_tag="VN084_07010"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWT29245.1"
                     /translation="MIPWLTSGAAFPSLSHALREPNGLLAAGADLSPARLLQAYRQGI
                     FPWYNPGQPILWWSPDPRMVLFPTELKVSRSLARRLRRTDYEIRVDSAFRQVMLHCAS
                     TPRPGQDGTWITPEIVEGYCRLHAMGYAHSVETWISGTLVGGIYGVAIGRMFYGESMF
                     HHVTDASKLAFVHLVRKLAESGFGMLDCQMQTTHLASMGAREISRDEFAQRLSELIHS
                     PHPPGTW"
     gene            complement(7279..7479)
                     /locus_tag="VN084_07015"
     CDS             complement(7279..7479)
                     /locus_tag="VN084_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HWT29246.1"
                     /translation="MYHYRVEDMSCGHCSSTITKAILATDKHAKVEIDLASKSVNVES
                     EEDFEVIREAIVEAGYSAAFVN"
     gene            7707..>7965
                     /locus_tag="VN084_07020"
     CDS             7707..>7965
                     /locus_tag="VN084_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HWT29247.1"
                     /translation="MARQTTSMLTGEADLAITGMSCAACVGRVEKALRKLPAVATVSV
                     NLATERAHVTLNAPTTADALEGAVSTAGYEAHWLGEGATASK"
BASE COUNT         1487 a         2309 c         2445 g         1724 t
ORIGIN      
        1 actggcgagg tggctgggca aaaaaagcgg cagcctccgg ggagactgcc gcttcggtat
       61 agctatcaac tattgagaca aatcaacgcg ttcgcgcaat tccttgccgg ccttgaagtg
      121 cggtacgtat ttctcgggta cctgcacctt ggtgccagtc ttcggattac gacccaggcg
      181 cggcggacgg taattcagac tgaaactacc gaaccccctg atttcgatgc gctcgcccgt
      241 tgcgagattg tccgacatgg agtccaggat cgccttcacg gaaagctcgg catccttgac
      301 caccagttgc ggaaaacgcg cagcaagctt ggcaatcagc tctgatttgg tcatcgcgcg
      361 taggccttat tcgttgttct tgccgtcaag cttggctttc agcagcgcac ccagattggt
      421 ggtgcctgca gcgccagcgt tgtcgttgct gaacttctgc atggcatccg cttcttcagc
      481 gatgtccttg gccttgatcg acaggttgat attgcggttc ttgcggtcga tgttggtgat
      541 gcgggcttcg atctcttcac cggccttcag cacgctggta gcgtcttcca ctttctcctt
      601 ggagatgtcg gacgcacgca ggtagccttc gacttcgccg tccagggtca cgacagcacc
      661 cttggcatct acggacttga cggtgccctt gacgatggcg cccttgtcgt tgaggctggt
      721 gtaagcgttg aaaggatcgc tgtccagttg cttgacgccg agggagatac gctccttctc
      781 gacatcgatc gccagtacga cagcagtgac ttcgtcaccc ttcttgaagt tgctgatcgc
      841 ttcttcgccg gactgattcc aggacaggtc ggacaagtgg acgaggccgt cgataccgcc
      901 aggcaagccg atgaacacac cgaaatcggt gatggacttg atctgaccgc tgaccttgtc
      961 acccttcttg tgggaggctg cgaagtcatc ccatgggttg gcttggcatt gcttcatgcc
     1021 gagcgacaga cgacgacggt cttcgtcgat ctcgaggatc atgacttcga cttcgtcacc
     1081 cagttgggcg atcttggcag gatgtacgtt cttgttggtc caatccattt cggagacgtg
     1141 gaccaggcct tcgataccag gctcgatctc gacgaacgcg ccgtagtcgg tcaggttggt
     1201 caccttgccg aacaggcggg tgctgacagg gtaacggcgg ctcagtgcaa cccatgggtc
     1261 gtcgcccagt tgcttgatgc cgagggatac gcgattcttt tcttgatcga acttgaggat
     1321 cttggcttcg acttcttcgc caacggtcag cacttcggat gggtgcttga cacgacgcca
     1381 tgccaaatcg gtgatgtgca gcaggccatc gatgccgccg aggtcgacga acgcaccgta
     1441 atcggtgatg ttcttgacga tacccttgac cactgcacct tccttcaggt tggccagcag
     1501 cgcttcgcgg tctgcgccca tgctctcttc gaggacggca cggcgggaga cgacgacgtt
     1561 gttacgcttg cggtcgagct tgatgacctt gaaatcccat tccttgttct cgaacggggt
     1621 ggtgtccttc acagggcgca ggtcgaccag cgaacctggc aggaaggcgc ggatgccgtt
     1681 gaccataacg gtcagaccac ccttgacgcg gccattgacc atgcccttga tgatacggcc
     1741 ttcgttcatg gcttcttcca gatccagcca tgccgcgagg cgcttggcct tttcgcgcga
     1801 caacttggtg gagccgtagc cgtcttccag tgactcgatc gcgaccttga cgaaatcgcc
     1861 aacggcgact tccagttcgc cacggtcgtt acggaattca ctggtagcaa tgatactttc
     1921 ggatttgagg ccggcattga cgatgataaa gtcatcatcg atcgacacga cttctgcggt
     1981 gatcacttca ccggagcgca tttcctggcg cgccaggctc tcctcgaata aagctgcgaa
     2041 actttcagtg gaggaggtgt tggtggaaac gttagccatt aaaaggggta cctaaataat
     2101 attcccgcct agcagcgggg ttgattgatg aaaccacgaa ccccagagat ccgtggtacc
     2161 tgcactacgt tactgcaact accgcattac tttgcccgag gtttcaggtg gtgggccagg
     2221 acgctgtcga cggcttgctc tatactgagc gcgctggttt caagcaattc ggcatcacga
     2281 ctctgctgca agggcgcaac cgatctttgt cggtctcggg catcgcgcgc ctgcaaatct
     2341 ttcaagatgt cggctagatt agcatggttt ccttttgaca tcaactgctt ataacgtcgt
     2401 tcggcccgca cttccgcgct tgccgtgagg aatattttgg tcccggcgtc cgggaaaacc
     2461 acggtcgcca tatcgcgtcc gtcggcgacc agtcccggcg ccttgcggaa gctgtgctgc
     2521 aggtcgagca gggccgctcg cactgccgga tgcaccgcga cctccgaggc tccccggctc
     2581 atctcttcgg tacgcaccgc ctcgctgatg tcttcattcc ccagccatat ccgtccctga
     2641 tcgaagcgga tctccagttt caacgccatg gctgcaagag caggttcgtc ttgccaggct
     2701 aagccttgcc tgcgtgcggc cagcgcgacg atgcgataga gtgcgccgga atccaggtag
     2761 tgataaccca gacgatctgc gacgatctgc gccacggtgc ccttgccaga ggcggacggg
     2821 ccgtcgatcg caatgactgg gatggaggta tcgttattca tcgatcaatt tttcttatct
     2881 ataagttttt aacaatcaat tagatttatc tggtgagctc gctatcatgg gggtgagtct
     2941 ttgcatctat cccaaggagc catgattatg actaaaaccc tcacctttgc cgctgtccac
     3001 ttctccgtgg cgttcacggt cggctacgca ttgaccggca gcgtcacggt tggtggtgcc
     3061 atcgcccttg tcgaacctgc atgcaacacg gtcgcctatt tcttccatga gaagatctgg
     3121 cagcgcatcg aacagggtga acacgggtcg cacgcgttgt tccatgccca ctagccgatt
     3181 agcccgctag tcgattagcc tactagcgga gtcgcccacc acgccgtcgg ggagcggcta
     3241 tcgctagccg attgacattg attgccatga cccccatccc acgcgagtga tcccaggcag
     3301 gcatcaggcc tgcgtgaccg cagcgaatcg ggtgaaatag tcggggaagg tcttggccac
     3361 gcagcccgga tcgttgatcg tgaccggcgt atggcccagc gctaccagtg aaaagcacat
     3421 cgccatgcgg tggtcgtcat aggtatcgat cttcgcattc ggcgtcagct gcgccggcgg
     3481 cgtgatcagc aggtaatcgc taccttcctc caccgtcgcg ccgaccttcc tcaactcagt
     3541 cgccatcgcg ctcagccggt ccgtctcttt cacgcgccag ctggcgatgt ttcgcaggcg
     3601 cgtcgggcca tccgcgaaca gcgcggcggt cgccagcgtc atcgccgcat ccgggatatg
     3661 gttgcagtcg agatccaccc cgcgcaactt gccagatgca ggcgcgctgg cctcaatcca
     3721 gttatcgccc caggcgatat tggcgcccat ggccgaaaga gcttcggtga atctgacgtc
     3781 gccctggatg ctgtccttgc cgatgccttc gacgcgcacc ggtccgccag cgattgcgcc
     3841 ggccgcgagg aaatacgaag ccgatgaggc gtcaccttcg acgtagacct tgcctgggct
     3901 ccgatagcga ctggctgcgg ggatagtgaa acgctgccag ccatcgcgcg cgaccactac
     3961 gccaaacctg gccatgaggt tgagcgtgat ctcgatataa ggcttggaga tgagttcgcc
     4021 gacgacttcg atcgtcgcct gcttgccggt cagcggcagg gccatgagca acgcggtgag
     4081 gaactggctt gagacatcgc cacggatgcg gatcgcttgc gtgacgtcga tcgccgctgg
     4141 ttcgatctgc aagggcgggt agccggcgtt accaaggtag gtgacctgcg cgccagcctg
     4201 gcgcaagccc tcgaccaggt cgccgatggg gcgctcatgc atgcggggca cgccggaaag
     4261 cttgtaactg ccatcggaga aagcgagcgc ggcggtcaat ggccggatcg ccgtgccggc
     4321 gttgcccatg aagagatcgg ctgacttgac cgggaacgtg ccgccagtgc cttccacctg
     4381 ccagtcatgc tcgccggttt ggctcagcgc gacgccgagc gccttcagcg catcgagcat
     4441 acggtcgaca tcgtccgacg ccagcagatc gtggatctgt gtcgtgcctt gcgccagtgc
     4501 tgccagcagc agcgtgcggt tggagatgct cttggagccc ggtaatcgga tgctgccgca
     4561 tgcccgggtc agggggctga ggtcgagtgt ttccatgtca gttcttgggt ggatcagtct
     4621 ttgtttgggg atagtcgttt gtgtgcccac gcgttccgtg cctggctggc acgctcgaac
     4681 agggcttgca ggccttgccc atcagcggct tgcaatagcg tccggatctt ctgtagttcg
     4741 gctgcgtagg catcgagttc gtcgaggagt gccggccggt tggcaaggct gatatcgcgc
     4801 cacatctcgg gcgaactgcc ggcgatgcgg ctgaagtcgc ggaagccact ggcggcgaaa
     4861 tcgaagagct gggctgcgtt gtcgcgtgcg gccagttcat cgaccagcgc gaacgccagt
     4921 aaatgtggca ggtgactgac tgcggcgaag atgccgtcat gttcggcggc tgacatggtc
     4981 gtgacatgcg cgcctgccgc tgtccagagg gtgcgtacca gcgctacggc gtcggcatcg
     5041 gtctccgccc ctggggtgag tacgatgttc ttgccgtcgt aaaggtcagc gcgggccgca
     5101 gtgacgccgc tcttctcggc gccggcgatc ggatggccgg gcacgaagcg tttgttgcgt
     5161 gcgcccagaa tgtcctgcgc gcatcgctgt acatctgatt tggtactgcc ggcatcggtg
     5221 acgaccgtat ttgctcccaa gtgcggcgcg atcttgcgca ggatatccgg ggtctgggcg
     5281 acgggtgcgg cgatcatcac gatgtcggcg ccggttaagg cgtcggcagc atcgtgggca
     5341 acggcgtcga tcacgccgag gtcgagtgcg gcctgcatat tatcgctttg acgcccgacg
     5401 ccgacgatct ggccgatcgc atgatggcgc ttgagcgcga gcgcgagcga cccgccgatc
     5461 aggccgacgc caaagatcgc gagtttacgt gtatgcaagg caaggttgga ttgcggctca
     5521 ttcatgcgcg gattgtaaca tgggcatgcc acgagctcgc gcgcactggg cgccatgact
     5581 ggttgcggcg cgccatagca aaacggcgtt ttagctggtt gagagctaaa acgccgcatg
     5641 cttggagatg acattgaata ggacgaaaac ttatgcgctt agttcaaatc ctgtccaaat
     5701 attttttatc catggctgaa tcccccccca ttgccgaatc caattgccga gtcgcatcgc
     5761 tgcatcccat ggctctcccg cattgttcga gttggcatgg caggtcttgc gcgcctgcag
     5821 caaacagctc agcctgcggc ggagtctggg tgcaggtgat gatgcaacgg ctgccattcg
     5881 ccgtcgatca ggccctcctg cggcaggaaa tgcttcttgt aatgcatctt gcggctctgg
     5941 tcgatccagt aaccgaggta tagatagggc aggccaagtg agcggcacca ctcgatcagc
     6001 cagagcacgt tgaacgtgcc gaagctgttg gaacggtctg aagcgtcgta aaaggtgtat
     6061 accgcagaga gcccgtcgcg gacgatatcc accacgctga cgatgcgcag cgcgtcattg
     6121 tcgcgaaact cgaccatcac gctctcgacg ttgctctgca ccaggaaatt acggtattgc
     6181 tcggcgcttt cggcatcgtc gccgttgtga tggcgggctg tctgatacgc cgtgtagagc
     6241 gcgaagtgtt cttcgctgta gcgcagctcg ctcaccgtca cacgcagatc gaggtgctgc
     6301 ttcagggcgc ggcgctggct gcggttcggc gtgaaacgat cgaccggcag gcgcaccggg
     6361 atgcaagcgt tgcagtgctc gcaatggggg cgataggcga acttgccgct gcgccggaac
     6421 ccgagctgga tcagcccgct gtaggcttcg gcgtcgatca ggtgatgcgg tgcggcgatg
     6481 agcgactggg cgagtttgcc ctctaggtaa ccacacgggt aaggtgtggt cgcatagaac
     6541 tgcaaacgct gtagcggcgg atcattcggt aatgtcacga cggtttacca tgtgccaggt
     6601 ggatgcggtg agtgtatcaa ttctgacaag cgttgggcga attcgtcacg cgaaatctcg
     6661 cgggcgccca tcgaggccag gtgggtggtc tgcatctggc aatcgagcat gccgaatccc
     6721 gattcggcaa gttttcgcac caggtggacg aacgccaatt tggaggcgtc ggtgacatga
     6781 tgaaacatgg attccccata gaacatgcgg ccgatggcga cgccgtagat gccgcccacc
     6841 agtgtgccgc tgatccaggt ctccacgctg tgcgcgtagc ccatggcatg caggcggcaa
     6901 tagccttcga cgatctccgg cgtgatccat gtgccgtcct gccccggccg tggggtcgac
     6961 gcgcaatgca acatcacctg ccggaatgca ctgtcgacac ggatctcata gtctgtcctg
     7021 cgcaggcgtc gagcaaggga gcgtgacacc ttgagttcgg tggggaacag caccatgcgt
     7081 gggtctgggc tccaccacag gatgggctgg cctgggttat accaggggaa gatcccttgc
     7141 cgataggctt gcagcaggcg tgccggtgag aggtctgcgc ccgctgccaa caagccgttc
     7201 ggttccctga gcgcgtggga tagcgacgga aacgccgcgc cagaggtcag ccaggggatc
     7261 atgcgcggcc aggcagggtc agttgacgaa agcggcggaa taccccgctt cgacgatcgc
     7321 ttcgcggatc acttcgaaat cctcctcgct ctccacattg accgatttgc tggcgaggtc
     7381 gatctcgacc ttggcgtgct tgtcggtggc gagtatggct ttggtgatcg tgctgctgca
     7441 atggccgcag ctcatatctt ctacgcgata atggtacatg gtcgttcctt gtcagtgggg
     7501 tggtcgatca ttttgatcct taccgccggg gcaaggtcaa gtatccggca agatcagcgc
     7561 atggtcgagt acctgtgctg gcgacctacg gcacataccc tgatatggcc tgcgtatctc
     7621 aatgccgggc caaatgcttg accttgacat catggaaagg tttaggctgt catattctta
     7681 agccacaatc aatgaaggag tcgtcagtgg caagacaaac gacatcgatg ttgaccggcg
     7741 aggcagacct tgcgatcacc ggcatgagtt gcgccgcctg cgttggccgg gtggagaagg
     7801 ctttgcgcaa gcttcctgcg gtcgccactg tcagcgtcaa tctagcgacc gagcgcgcgc
     7861 atgtcacgct caatgcgccg accaccgcgg atgcgctgga aggcgccgtc tccactgccg
     7921 gctatgaggc gcactggctg ggcgaagggg ccacggccag caagg
//
LOCUS       DATMTW010000358         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_534329, whole genome shotgun sequence.
ACCESSION   DATMTW010000358 DATMTW010000000
VERSION     DATMTW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534329"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VN084_07025"
     CDS             complement(<1..231)
                     /locus_tag="VN084_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWT29248.1"
                     /translation="MALRLTLYSTSACHLCEQAEQLLEALPPTLACWQTIEITEDEAL
                     LAGYGTRIPVVRREDTQAELGWPFDTAQLLAFL"
     gene            372..1808
                     /gene="mgtE"
                     /locus_tag="VN084_07030"
     CDS             372..1808
                     /gene="mgtE"
                     /locus_tag="VN084_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWT29249.1"
                     /translation="MAEIQESKESLQESLQQVITLLNKHRLVEGLVHKQDMPKHELVE
                     SLVHKQNLAELHNKLDALHPADVAYILEALPLEQRLDVWDMVKAERDGEILLEVSDAV
                     RQTLIADMDSDELLAAAEQLDTDELADLAPDLPKDVLQDLLDSLDIQNRERLQSALAY
                     PEDAVGALMDFDIVTIREDISLEVALRYLRRIGSLPDHTDKLFVVDRHDILRGVLPLK
                     RLVVSDLDLGVAEIMAGDAVVFHPEDKADEAAKAFERYDLVTAPVVDENNKLVGRITV
                     DAVMDFIRDEAESDMLSMAGLREEEDLFSSVWKSVQNRWSWLAINLITALVASRVIGL
                     FEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRGLALGQISSHNMQSLLKKELGVS
                     LMNGLIWGSVLGLVAYWLYGNVALGVVMMSAMTLNLLLSAVMGVLIPLLMNRSGRDPA
                     VGSSVLITAMTDSGGFFIFLGLATIFLI"
     gene            1826..3109
                     /locus_tag="VN084_07035"
     CDS             1826..3109
                     /locus_tag="VN084_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWT29250.1"
                     /translation="MWQEFLGDLATPAFAWQIGMLVASLLLAWSINGLLRAYVMRRAP
                     ETWKVAIGGINRVLFPLSTLAFVTVGKLLLSYWEHTSLLRLATILLIAMAVIRLAVYA
                     LRFIFAPSGWLKTMESAISTTVWILLALHLSGLLPQVLMVLEDVSFRIGKSPVNLLQV
                     LQALLTILVTLFFALWLSRILENKLMRSEHLGINMRVVLSKVVRIALSFTAILMALSA
                     VGLDITLLSVFGGALGVGLGFGLQKIASNYVSGFIILTDKSMQIGDVITIDSHYGVVD
                     DLRSRYMVLRKLDGTQVIIPNENLITNPVINHSLAEHKARVQLFVQVSYESPLKLAMR
                     LMCEAADKQPRVLVEPKPEARVEAFGESGIDLSLNIWIPDPEEGSASLRSAIYLDIWE
                     AFQANGISIPYPQREIRILGTVHTSSGDQSGKGID"
     gene            3135..>4306
                     /locus_tag="VN084_07040"
     CDS             3135..>4306
                     /locus_tag="VN084_07040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWT29251.1"
                     /translation="MEFWWITLPWWGYVLVALGLTHVTIAAVTIYLHRCQAHRALDLH
                     PAVSHFFRFWLWLTTGMVTKEWAAIHRKHHARCETAQDPHSPQVLGIAKVLREGAELY
                     RSEAGKQETMDKFGSGTPDDWLERHVYTRRNGLGIYLMMAIDIGLFGPIGLTIWAVQM
                     AWIPITAAGIINGIGHYWGYRNYAADDASTNILPWGILIGGEELHNNHHSYASSARLS
                     SKWYEFDIGWLYIRTMQAAGLAKVKKVAPRIRFDWAKTRCDLDTLQAVITHRFDVMGK
                     YGRLLKKVYKEEIHKIHAAYQHHGQESVTMERIRHWLHLDAAHLHEQEKAALQQALQH
                     STRLQTLYQLKQDLSAIWQRSAVTQEQLLKHLEDWCHCAEASGIQALEDFSYRLRC"
BASE COUNT          885 a         1213 c         1268 g          940 t
ORIGIN      
        1 gaggaaggcc agcagttgtg ctgtatcgaa tggccagcca agttcagcct gcgtatcttc
       61 acgcctgacg accgggatgc gggtgccata gcctgccagc aacgcctcat cttcggtgat
      121 ctcgatcgtt tgccagcagg ctagcgttgg gggcaaggcc tccaaaagct gctccgcctg
      181 ttcgcaaaga tggcaggcag aggtgctgta cagagtgagt cggagagcca agcgcgtgtt
      241 tccggtgagg gcaggagagc gcaacaaaat tcgacgttaa cgttttcgtg gcgcataaaa
      301 aacaccataa tagcaggtgt tgcgagcgct gaatccagaa caagtttact taattctctg
      361 cgggctgacg aatggctgaa attcaagaat caaaagaaag cttacaagag agtctgcaac
      421 aggtgatcac cctgctcaac aagcacaggc tggtcgaagg cctcgtgcac aagcaggata
      481 tgcccaagca cgaacttgtc gaatctctcg ttcataaaca gaacctcgct gagctgcaca
      541 acaagctgga tgcgctgcac ccggccgacg tcgcctacat ccttgaggcc ttgccgctcg
      601 agcagcggct tgatgtctgg gatatggtca aggccgagcg cgacggcgag atcctgcttg
      661 aagtctccga tgcggtccgc cagaccctta tcgccgacat ggattcggac gaactgctgg
      721 ctgccgccga acagctcgat accgacgaac tggccgacct ggcgcctgac ttgccaaagg
      781 acgtgctcca ggatttgctg gacagcctgg atatccagaa ccgtgagcgc ctgcaatccg
      841 cgttggccta tcctgaagat gctgtcggcg cgctcatgga cttcgatatc gtcaccatcc
      901 gcgaagacat cagtctcgaa gtggcgttgc gttacctgcg ccggatcggc agcctgccgg
      961 atcacaccga caagctgttc gtggtcgacc gtcatgacat cctacgtggc gtactacccc
     1021 tgaagcggct ggttgtcagc gacctcgacc tcggcgtggc ggagatcatg gccggtgacg
     1081 cagtcgtttt tcatccggaa gacaaggccg acgaagcggc caaggctttt gagcgctatg
     1141 acctcgtgac tgcgccggtg gtcgacgaga acaacaagct ggttggacgt atcaccgtcg
     1201 atgcggtcat ggacttcatc cgggatgaag ctgaaagtga catgttgtcg atggctggct
     1261 tgcgcgagga agaggatctg ttctcttcgg tctggaagtc ggtgcagaac cgctggtcgt
     1321 ggctggccat caacctcatt acggcactgg tggcgtcgcg cgtcattgga ctctttgaag
     1381 gctcgatcga gaagattgtt gcattggcgg cgttgatgcc tatcgtggcc ggcatcggcg
     1441 gcaactcggg taaccagacc atcaccatga ttgtacgtgg cctggcgcta ggccagatct
     1501 cgtcgcacaa tatgcagtcg ctgctcaaga aggaactagg cgtgagcctg atgaacgggc
     1561 tgatctgggg cagcgtgctg gggctggtcg cttactggtt atacggtaac gtggcattgg
     1621 gtgtggtcat gatgagcgcc atgactctca acctactgct ctccgccgtg atgggtgtcc
     1681 ttatcccgct actcatgaat cgctccggcc gtgaccccgc agtcggatcc agcgtactca
     1741 tcactgccat gaccgatagc ggcggcttct tcatcttcct tggtctcgcc accattttcc
     1801 tgatatgaat cccgagatgc acctgatgtg gcaagaattc cttggcgacc tcgcgactcc
     1861 ggcgttcgcc tggcagatcg gtatgctggt tgcgagcctg ctgctggcat ggtctatcaa
     1921 tggcttgctt cgcgcgtatg tcatgcgacg cgcgccggag acctggaagg tggcgatcgg
     1981 tggcatcaat cgcgtgttgt tcccgctgtc caccttggcg ttcgtcacgg tcggcaagct
     2041 gctgctgagc tattgggagc atacgagctt gctgcggcta gcgacgatcc tgctcatcgc
     2101 catggcggtg atccggctcg cggtttatgc cctgcggttc atcttcgcgc ccagcggctg
     2161 gctcaaaacc atggagagtg cgatctcgac caccgtctgg attctgctcg cgttgcactt
     2221 gagcggtttg ttgccgcaag tgctgatggt gctggaagat gtcagcttca ggatcggtaa
     2281 gagcccagtc aacctgctgc aggtattgca ggcgctactg acgatccttg tgaccctgtt
     2341 cttcgccttg tggctgagcc gcatccttga gaacaagctg atgcgttccg agcatctcgg
     2401 catcaatatg cgcgtggtcc tgagcaaggt cgtgcgtatc gcactctcat tcacggccat
     2461 tttgatggcc ctgtcggctg tcgggctcga cattaccctc ttgtcggtgt tcggtggggc
     2521 cttgggtgta ggtctcggct tcggtctgca gaagatcgcc agcaactatg tcagcgggtt
     2581 catcattctg accgataaat cgatgcagat cggagacgtg atcaccatcg acagccacta
     2641 tggcgttgtg gatgacctac gttcgcgcta tatggtgctg cgcaaactcg atgggacaca
     2701 ggtcatcatc cccaatgaga acctgatcac caacccggtc atcaatcatt cactggccga
     2761 gcacaaggcg cgcgtccagc tattcgttca ggtcagttac gagagcccgc tcaagctggc
     2821 gatgcgcctc atgtgcgagg ctgcagacaa gcagccgcga gtgctggtcg aacccaagcc
     2881 ggaagcgcgc gtcgaggcgt tcggcgagag cggtatcgat ctcagcctga atatctggat
     2941 ccccgatccg gaggagggtt cagcgagcct gcgttcagcc atctatctcg acatctggga
     3001 agccttccag gccaacggca tctcgatccc ctacccgcag cgcgagatcc gtatcctggg
     3061 caccgtgcat accagtagtg gcgatcagtc gggaaaaggt atagattgag tcaagcaaac
     3121 aacttggagt attgatggaa ttctggtgga tcacacttcc ctggtggggc tatgtattgg
     3181 tggcacttgg cctgactcat gtcacgatcg ccgcagtgac catttacctg catcgttgcc
     3241 aggcgcaccg tgcactcgat ctgcatccgg ctgtgagtca tttcttccgt ttctggctct
     3301 ggcttacgac gggtatggtc accaaggagt gggcagctat ccaccgcaag catcatgcgc
     3361 gttgcgagac tgcacaagat ccgcatagcc cgcaagtcct cggcatcgca aaagtcctcc
     3421 gtgaaggcgc cgagctatac cgctccgaag ccggcaagca ggagaccatg gacaagttcg
     3481 gcagtggcac gccggatgac tggctggagc gtcatgtcta cacccgtcgt aacggcttgg
     3541 gcatctacct gatgatggcg atcgacatcg gcttgttcgg ccccatcggg ctgacgatct
     3601 gggccgtgca gatggcctgg atccccatca ctgccgctgg catcatcaat ggtatcggac
     3661 actactgggg ctaccgtaac tatgcggcgg atgatgccag taccaatatc ctgccttggg
     3721 gtatcctcat cggcggcgaa gaattgcaca acaatcatca ctcctatgcc agttccgcgc
     3781 ggctctccag caaatggtac gagttcgata tcggctggct gtatatccgc accatgcagg
     3841 ccgctggtct cgccaaggta aagaaggtcg cgccccgtat ccggttcgac tgggccaaga
     3901 cccgctgcga cctcgatacc ctgcaggctg tgatcacgca tcgtttcgac gtgatgggca
     3961 agtacggtag attgttgaag aaggtctaca aggaagagat ccacaagatc catgcggctt
     4021 accagcacca tggtcaggaa tccgtgacca tggagcgcat ccgacactgg ttgcatctcg
     4081 atgcggcgca cctgcacgaa caggaaaagg ctgccttgca gcaggcgctg caacacagta
     4141 cgagactgca aaccctgtat cagctgaagc aggacctgag cgcgatctgg cagcgttctg
     4201 cggtgacaca ggaacagttg ttgaagcatc tggaagactg gtgccattgt gcggaagcga
     4261 gcggtatcca ggctctggag gacttctcct accgtctacg ctgtta
//
LOCUS       DATMTW010000359         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_199015, whole genome shotgun sequence.
ACCESSION   DATMTW010000359 DATMTW010000000
VERSION     DATMTW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199015"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="ubiB"
                     /locus_tag="VN084_07045"
     CDS             complement(<1..1195)
                     /gene="ubiB"
                     /locus_tag="VN084_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HWT29252.1"
                     /translation="MRFLRLFRIFTVTLRFGLDEFILSHTKLRACQRLLHGLLFWRDV
                     SQPRGVRLRLAFEALGPIFVKFGQMLSTRRDIIPLDIANELAKLQDRVPPFPYAQVQR
                     TIETAFEQPLTTLYRQFSEAPVASASVAQVHFAELPDGTPVAVKVLRPGIGAVIEKDL
                     ALLDAAAWLVHKLWHEGKRLKPREIVAEFARHTHAELDLMLEAANCSQLRRNFANSKM
                     LLVPDVHWDWCRHEVMTMERMQGTPVSQVDALRAAGVDIPKLARDGVEIFFTQVFRDG
                     FFHADMHPGNIQVATDQAHLGRYIALDFGIMGTLNDTDKEYLARNFLAFFRRDYREVA
                     KAHVESGWVPADTAIDEFETAIRAICEPIFDKPLKEISFGRTLLALFQMARRFGVIIQ
                     PQLIML"
     gene            complement(1202..1807)
                     /locus_tag="VN084_07050"
     CDS             complement(1202..1807)
                     /locus_tag="VN084_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWT29253.1"
                     /translation="MFKHILTRLLNHLVNQNAWAREQLAPHGGKSVRFLVPPVDTTLT
                     ILEDGGLAMAGETALADATVTIPLPVALRLLANDDSATTLASLEGDTELAATLAKVLR
                     GMHWEYEEDLSRIIGDAPAHELAALGRKTVDGVRKQSLNVAEMFAEYWQEEQPLIAKK
                     RHVMHFVEAVDTLREDTERLEKRLESLARKVADSTSPGKKD"
     gene            complement(1814..2551)
                     /gene="ubiE"
                     /locus_tag="VN084_07055"
     CDS             complement(1814..2551)
                     /gene="ubiE"
                     /locus_tag="VN084_07055"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HWT29254.1"
                     /translation="MSDNTTHFGFKTVDEKEKAHKVGEVFHSVARKYDLMNDVMSAGM
                     HRGWKRFAVEVSGVREGDRVLDIAGGSGDLSKLFARKVGDSGEVILTDINASMLSVGR
                     DRMIDAGLAVPTMLCDAEQLPFPDQHFDCVMVAFGLRNMTHKDRALAEMQRVLKVGGR
                     LLVLEFSQIWKPLAPIYDAYSFKLLPRMGKLFAKDAESYQYLAESIRMHPDQETLKQM
                     MQDAGFAKVDYYNMAAGAVALHKGWKL"
     gene            complement(2570..2947)
                     /locus_tag="VN084_07060"
     CDS             complement(2570..2947)
                     /locus_tag="VN084_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWT29255.1"
                     /translation="MSGLNPTTPRPVDIRLHQASRLLELRFDNGTEAMLSCELLRVYS
                     PSAEVRGHGIGQEVLQIGKEFVNITGVEPVGNYAVRLIFSDGHDTGLYSWDYLYDLAR
                     NYESLWQVYLSRLDAAGHVRVAG"
     gene            3161..>3979
                     /locus_tag="VN084_07065"
     CDS             3161..>3979
                     /locus_tag="VN084_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HWT29256.1"
                     /translation="MAENKLGQQFAAYTQWRKALVDTICDYRSWLNEQELNDAQIDQR
                     ISHLLDRLREDRLNVAFVAEFSRGKSELINAIFFADYGQRLLPSSAGRTTMCPTELMY
                     DPAKPTSVQLLPIETRASDATTTEYKRYPEEWKVFEFNVDNNDSMIEAFKEVSRIKTV
                     TQDEAQRYGLFDPTSSDDAMSLNSDGTINIPSWRYAMINFPHPLLEQGLVILDTPGLN
                     AIGTEPELTLNMLPNAHAVLFILAADTGVTKSEIDVWRQYISGTRWKQKGRLAVL"
BASE COUNT          807 a         1155 c         1203 g          814 t
ORIGIN      
        1 gcagcatgat gagttgcggc tggatgatga cgccgaaacg gcgggccatc tggaacaacg
       61 ccagcaaggt gcgcccgaat gagatctctt tcagtggctt gtcgaagatc ggttcgcaga
      121 tagcgcggat cgcggtctcg aactcatcga tggcggtatc ggcaggcacc catccggatt
      181 cgacgtgggc ttttgccacc tcgcgataat cacgccggaa gaacgccagg aagttacggg
      241 ccaggtattc cttatccgta tcgttgagcg tgcccatgat gccgaaatcc agcgcgatat
      301 agcgaccgag gtgggcctgg tcggtggcga cctgaatgtt gcctggatgc atgtcggcat
      361 ggaaaaagcc gtcacggaag acttgggtga agaagatctc gacgccatcc cgggccaatt
      421 tggggatgtc gacaccggcg gcgcgcagcg cgtcgacttg actcaccggc gtgccttgca
      481 tgcgttccat ggtcatgact tcgtggcggc accaatccca gtgcacatcc ggcaccagca
      541 gcatcttgct attggcgaaa ttgcgccgta gctggctgca gttggcggcc tccagcataa
      601 gatcgagttc ggcatgggta tgacgcgcga attcggcgac gatctcgcgt ggtttcaggc
      661 gcttgccttc gtgccagagt ttatgcacga gccaggcggc ggcgtcgagt agcgcgaggt
      721 ctttctcgat cacggcaccg atgcccggac gcagtacctt gactgctacc ggcgtgccgt
      781 caggcagctc ggcaaaatgg acttgtgcga ctgaagcgct ggccaccggg gcctcggaga
      841 attgccggta gagtgtggtc agcggttgtt cgaacgcagt ctcgatggtc cgctggacct
      901 gcgcgtaagg gaaaggcggt acacgatcct gcagcttggc gagctcattg gcgatatcga
      961 gcgggatgat gtcgcggcgt gtcgagagca tctggccgaa cttgacgaag atcggcccca
     1021 gcgcctcgaa ggccaaacgc aggcgaacgc cacgcggctg gctgacgtcg cgccagaata
     1081 gcaggccatg caggagccgt tggcaggcac gcagcttggt gtggctgagg atgaattcgt
     1141 cgagcccgaa gcgcagggtg accgtgaaaa tcctgaaaag acgcaggaag cgcatggatg
     1201 ctcagtcttt cttgcccggc gacgtgctgt cggcgacttt tctcgcgagg ctctccaacc
     1261 gtttctccag ccgttcggta tcttcgcgca gggtatcgac tgcttcgacg aaatgcataa
     1321 cgtggcgctt cttggcgatc agaggttgtt cttcctgcca gtattcggcg aacatctcgg
     1381 cgacattgag cgactgcttc ctgacgccat caactgtctt gcggccaagg gcggccagct
     1441 catgggccgg cgcgtcgccg atgatgcggc tcaggtcttc ttcgtattcc caatgcatgc
     1501 cgcgcagcac tttggccagt gtggccgcca gttcagtgtc gccctccagg ctggcaagcg
     1561 tggtggcgct gtcgtcattg gcgagcaggc gcaaggccac gggtagcgga atggtgactg
     1621 tggcgtcggc gagggcggtt tctccagcca tggcaagacc gccgtcctca aggatggtga
     1681 gtgtcgtgtc cacagggggt acgaggaagc ggacgctctt gccgccgtgt ggcgcgagtt
     1741 gctcgcgtgc ccaggcgttc tggttgacga gatgattgag caggcgcgtc aggatgtgct
     1801 tgaacatagg cgcctagagc ttccagcctt tatgcagggc gaccgccccg gccgccatgt
     1861 tgtagtaatc cactttggcg aaaccggcgt cttgcatcat ctgcttcagg gtctcctggt
     1921 ccgggtgcat gcggatggat tcggccagat actggtagct ctctgcatcc ttggcgaaga
     1981 gcttgcccat gcgtggcaac agcttgaagg agtaggcgtc gtagatcggc gccaggggct
     2041 tccagatctg cgagaactcc agcaccagca gacgcccacc aactttgagc acacgttgca
     2101 tctcggccag ggcacggtcc ttgtgcgtca tgttgcgcag accgaaggcc accatgacgc
     2161 aatcgaaatg ctggtcggga aagggcaact gctcggcgtc acagagcatg gtgggtacgg
     2221 ccaggccagc atctatcatg cgatcgcgac cgacgctcag catggaggcg ttgatatcgg
     2281 tcaggatcac ctcgccgcta tcccccacct tgcgcgcgaa cagcttggac aggtcgccgc
     2341 tgccgcctgc gatgtccagc acacggtcgc cctcgcgtac gccgctgact tcaaccgcga
     2401 aacgtttcca gccgcgatgc atgccggcgg acatgacgtc gttcatgaga tcatatttgc
     2461 gtgcgacgga atggaagact tcgccgacct tgtgggcctt ttccttttca tcgacggtct
     2521 tgaaaccgaa atgggtggtg ttgtcgctca tgccgttcct ttacaaacgt tatcctgcca
     2581 cacgtacatg gccagccgcg tcaaggcggc tgaggtatac ctgccagaga ctctcgtagt
     2641 tcctggccag gtcgtaaaga taatcccagg agtagagccc ggtatcgtgg ccatcggaaa
     2701 agatgagccg cacggcataa ttgccgaccg gctcgacgcc agtgatgttg acgaactcct
     2761 tgccgatctg cagcacttcc tgacctatac catgtccacg gacttccgcc gatggcgagt
     2821 agacacgcag gagttcgcac gagagcatgg cttccgtgcc gttatcgaac ctgagctcca
     2881 gcaggcgcga tgcttgatgc aggcggatat cgacgggacg cggggtcgtg gggttgaggc
     2941 cgctcataag actgctgttt ccgaaaacga cgatgatagc agaatgtgcc cagcgggtct
     3001 gcgcgtggcg ccgccttccg cggcattttc aaccgttaat cgcaactttg gctccgctta
     3061 tctgaaaacc gcatgaaatt tggccattag ccgtatgatt aatttgacta ttttaggcaa
     3121 actgctacag ttgcagcaaa cgaaggcatc gggaacggac atggctgaga ataagctggg
     3181 acaacagttc gctgcatata cgcagtggcg caaggcattg gtcgatacca tctgcgatta
     3241 ccgcagttgg ctcaatgagc aggagctcaa cgatgcccag atcgatcagc gcatctcaca
     3301 cctgctcgac cgcctgcgcg aagaccgctt gaatgtcgct ttcgtcgccg aattctcacg
     3361 cggcaaatct gaactcatca acgccatctt cttcgctgac tatggtcagc gcctgctgcc
     3421 gtcgagtgcc ggacgtacca cgatgtgccc gaccgagctg atgtatgacc cagccaagcc
     3481 gacttcggtg caattgctgc cgatcgagac acgcgcctcc gacgccacta ccaccgaata
     3541 caagcgttac ccggaagaat ggaaggtctt cgaattcaat gtcgacaaca acgacagcat
     3601 gatcgaagcc ttcaaggagg tcagccgcat caagaccgtg acgcaggacg aagcccagcg
     3661 ctacggcttg ttcgacccga ccagctccga tgacgccatg agcctcaata gcgacggcac
     3721 catcaatatc ccgagctggc gctatgccat gatcaacttc ccccatccct tgctcgaaca
     3781 ggggctggtc atcctcgata ctcccggtct caacgccatc ggtaccgagc cagagctgac
     3841 gcttaacatg ttgccgaatg cccatgccgt gcttttcatc ctggctgcgg ataccggtgt
     3901 caccaagtcc gagattgacg tctggcgtca gtacatcagc ggcacccgtt ggaagcagaa
     3961 gggccgactc gcggttctc
//
LOCUS       DATMTW010000360         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_367458, whole genome shotgun sequence.
ACCESSION   DATMTW010000360 DATMTW010000000
VERSION     DATMTW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367458"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..425
                     /locus_tag="VN084_07070"
     CDS             51..425
                     /locus_tag="VN084_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWT29257.1"
                     /translation="MIHIKQLMHAGLIVSDLKRSREFYEGLLGLAPNPNRPDFSFEGV
                     WYDIGANQLHLMVVPNPYEHVTRPEHGGRDNHVAFSVEDVAPIREALDKAGVAYSMSK
                     SGRAAVFCRDPDGNALEFSAVR"
     gene            complement(578..2368)
                     /gene="feoB"
                     /locus_tag="VN084_07075"
     CDS             complement(578..2368)
                     /gene="feoB"
                     /locus_tag="VN084_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HWT29258.1"
                     /translation="MKRIALIGMPNTGKSTLFNRISGASARVGNWPGITVDLMSAKIL
                     LGGHIVELIDLPGLYDLHGYSDDEQVVRDFLATNAVDLVMITLNGSQIDRQLSLALQI
                     RELGLPAILMLNMVDESRRNGITIDTPVLSRQLNMPVMQISAKHGEGTAEALRLAAKH
                     LDQQTGTVSPEQISQLLHEDHRLVEEMEALVKDTVHIPAQLNDQLTARIDHLLLHPWF
                     GIPIFFAAMLLLFQFIFTAGAPLQDGVAWLLDTLRVDVLEPLFTGAPAWLKGLLLDGI
                     YNGVGTVAAFIPIIILFFLIMTMVEDSGYLSRAAFLMDTLMAKMGLDGRGFVMMLMGF
                     GCNVPALMGTRVMRSRALRLLTMLVIPLSLCSARLQVFVFITAILFSPSKAPFVLFFL
                     YLASFAAIFLTALLFKGKYASHEPFILELPPYRFPTPRQVWLRGWHEVRHFLHRATKF
                     IVIGVVMVWLLTNFPPGVHPASSETLAGQIGTFFAPVLSPIGIDTQLAIALIFGFVAK
                     EIVVGSLAVIYGLQGDALMHLMGQQLDWVQGMSFMLFTLIYTPCLSTVATLKSESKST
                     GFMLLSIGWSLALAWVVSFIFYQSARALGF"
     gene            complement(2365..2607)
                     /locus_tag="VN084_07080"
     CDS             complement(2365..2607)
                     /locus_tag="VN084_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HWT29259.1"
                     /translation="MTSLDLLPPGENGIIRSITADASLQKRLAALGFRIGKRVELIRR
                     ASFNGPLHVRLGTTDVILRAREAQLIEISTSTQAQQ"
     gene            complement(2715..>3384)
                     /locus_tag="VN084_07085"
     CDS             complement(2715..>3384)
                     /locus_tag="VN084_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWT29260.1"
                     /translation="WIKLYKPDENTPNAQVSYYLKGSLTALALDLTLRQARHSLDEVM
                     HRLWQLHGDSGIGLAEGALADLVRNVTGVDVDALLDSALRSTEALPLERLLGDFGIDY
                     QETVDQERTTDLGIKLGPGEEARIATVLSGSPAERAGLAGGDVVIAVDGLRVNNKTLE
                     KRTGKRKAGSTITVHAFRRDELMCFEVTLTAARPDLCKLAVKRRIGLAMRARQDAWLG
                     PASA"
BASE COUNT          673 a          998 c         1067 g          646 t
ORIGIN      
        1 gtgcctcatg ggggccgtct atttcatgtt cagaaccacg acataaacct atgatccata
       61 tcaagcaact catgcatgcc ggcctgatcg tctctgatct caagcgctcc cgtgaattct
      121 acgaaggcct gctcggcctt gcgcccaatc ccaatcgccc ggatttcagt ttcgagggcg
      181 tctggtacga catcggcgcc aatcagctcc acctgatggt ggtgcctaac ccctatgagc
      241 atgtgacgcg cccggaacat ggcggccgtg acaaccatgt ggcattctcg gtggaagatg
      301 tcgcccccat cagggaggcg ctagataaag ctggcgtggc ctacagcatg agcaagtccg
      361 gtcgtgccgc ggtgttctgc cgcgacccgg atggtaatgc cttggagttc tcggccgtca
      421 ggtgaccctg tagcctgggt ttgcgctgtt caatggtgac tttgcgcagc acgaattttc
      481 cggctgtaat tcctatgtgg gctggtactt gcggtctcta gcccgtgtgg cctcaaaaat
      541 gcgctactgg catcgacgcc ctggcgtaac cccgtgatca aaaccccaaa gcgcgcgccg
      601 actggtagaa gataaagctt accacccagg ccagtgccag cgaccagcca atggagagca
      661 gcatgaagcc ggtgctcttc gattccgatt tcagtgtggc cacggtggac aggcagggcg
      721 tgtagatcag cgtgaacaac atgaagctca tgccttgcac ccaatcgagt tgctggccca
      781 tcaggtgcat cagcgcatcg ccctgcaaac catagatcac ggccagcgag ccgaccacga
      841 tctccttggc gacgaagccg aagatgagcg cgatcgcgag ctgggtgtcg atgccgatcg
      901 gactgagcac cggcgcgaag aaggtgccga tctggccggc cagcgtttcg ctgctggcgg
      961 ggtgcacgcc tggcgggaag ttggtcagca gccagaccat gaccacgccg atgacgatga
     1021 acttggtcgc ccggtgcagg aagtgccgca cttcatgcca gccgcgcaac cagacctgtc
     1081 ttggcgtcgg gaagcggtag gggggcagtt ccaggatgaa gggctcgtgg ctggcatatt
     1141 tgcccttgaa cagtagcgcc gtgaggaaga tcgcggcgaa gctcgcgagg tagaggaaga
     1201 acagcacgaa cggcgccttg ctcgggctga acaggatggc cgtgatgaaa acgaacacct
     1261 gcaggcgcgc cgagcacagc gagagcggga tcaccagcat ggtcagcagg cgcagcgccc
     1321 gcgagcgcat gacgcgtgtg cccatcagcg ccggcacgtt gcagccaaat cccatcagca
     1381 tcatgacgaa gccgcggccg tcgagcccca tcttggccat cagcgtgtcc atgaggaaag
     1441 ccgcgcgcga cagatagccg ctatcctcga ccatggtcat gatcaggaag aacaggatga
     1501 tgatgggaat gaacgccgcc acggtgccga ccccgttata gatcccgtcg agcagcaaac
     1561 ccttgagcca ggccggggcg cccgtgaaca gcggttccag gacatccacg cgcagggtgt
     1621 cgagcagcca ggccacgcca tcctgcaacg gagcgcccgc ggtgaagatg aactggaaca
     1681 gcagcagcat cgcggcgaag aagatcggga ttccgaacca tggatgcagc agcaggtggt
     1741 cgatgcgtgc ggtcagctgg tcgttgagtt gcgccgggat atgcaccgta tccttgacga
     1801 gcgcttccat ctcttccacc agtcggtggt cttcatgcag tagttggctg atctgctccg
     1861 gcgataccgt gccagtctgc tggtcaagat gcttggccgc cagacgcagc gcttcggcag
     1921 tgccttcgcc atgcttggcg ctgatctgca tcactggcat gttgagttgc cgcgacagga
     1981 ctggcgtatc gatggtgatg ccgttgcgcc gagattcgtc caccatgttc aacatgagga
     2041 tggcgggcag gccgagttct cgaatctgca gggcgagcga aagctggcga tcgatctgcg
     2101 agccgttgag cgtgatcatg accaggtcaa cggcgttggt cgccaggaaa tcgcgcacca
     2161 cctgctcgtc gtccgaatag ccatgcagat cgtagaggcc gggcaggtcg atcagctcga
     2221 cgatatgtcc gccgagcagg atcttcgcgc tcatgaggtc gaccgtgatc cccggccagt
     2281 tgccgacgcg cgccgacgca ccgctgatgc ggttgaacag ggtcgatttg cccgtattgg
     2341 gcatgccgat gagggcgatg cgtttcattg ctgagcctgg gtggaggtgc tgatctcgat
     2401 gagttgcgct tcgcgtgcgc gcaagatgac gtcggtcgtg cctagcctga catgcagcgg
     2461 cccgttgaaa ctggcgcggc ggatcagttc gacccgttta ccgatgcgaa agccgagcgc
     2521 cgccagccgc ttctgtagcg aagcgtccgc cgtgatactg cggatgatgc cattttcacc
     2581 tgggggaagc aggtcaagac tggtcacgag ggactcgcat ataggtaaat aagaacaatt
     2641 cttattatcc catagattgt cggacaagca tatcgtgccc atgcgcatgg ccatatgcat
     2701 gcaaagcgat gcgatcaggc tgaagccggg ccgagccagg catcctggcg ggctcgcatg
     2761 gcaagcccaa tgcggcgctt gacggccagt ttgcacaggt caggacgggc ggccgtcagc
     2821 gtcacctcaa agcacatcag ttcatcgcgg cgaaatgcat ggacggtgat ggtgctaccg
     2881 gccttgcgct tgccggttcg cttctcgagg gtcttgttgt tgacgcgtaa gccatcgact
     2941 gcgatcacga catcgccacc ggcgagcccg gcgcgctctg cagggctgcc gctcagcaca
     3001 gtggcgatcc tggcttcttc gcctgggccc agcttgatac cgagatcggt ggtccgttcc
     3061 tgatcgaccg tctcctggta gtcgataccg aaatcgccca gcaggcgctc gagcggtagt
     3121 gcctcggtgc tgcgcagggc cgagtccagc aaggcatcga cgtcgacgcc ggtgacgtta
     3181 cgcaccaggt cggccagcgc gccttcggca aggccgatgc cgctatcgcc atgcaactgc
     3241 catagcctgt gcatcacttc gtcaaggcta tgtctggcct gccgcagcgt cagatccagc
     3301 gccagcgcgg tgagcgaacc tttgagatag tagctgacct gggcgttggg cgtgttctcg
     3361 tccggcttgt agagcttgat ccag
//
LOCUS       DATMTW010000361         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_367514, whole genome shotgun sequence.
ACCESSION   DATMTW010000361 DATMTW010000000
VERSION     DATMTW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367514"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..300
                     /locus_tag="VN084_07090"
                     /pseudo
     CDS             1..300
                     /locus_tag="VN084_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480424.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            319..1125
                     /locus_tag="VN084_07095"
     CDS             319..1125
                     /locus_tag="VN084_07095"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWT29261.1"
                     /translation="MHHRTVFFISDGTGLTAQALGQLLAHFPNTEFRQIRLPFTNTLQ
                     QVYEAMARIDMARAEDNVPPVVVMSLGNVDHRSLIKQCTAYYIDLFESFINPLGIELN
                     ETPLTRAGIAHSIQGQNYHERMEAINFTLGHDDGMTEEGLEEAQVILIGVSRCGKTPT
                     SLYMAMQFGIKAANYPLIPEDLERGRIPSVLENFPHKLFGMTIKPERLHSVRSERRPN
                     SFYASLENCRNEVRMAEEMMRNSGVHWIDSTSRSIEELSTTILQELKLSN"
     gene            1190..>2071
                     /locus_tag="VN084_07100"
     CDS             1190..>2071
                     /locus_tag="VN084_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HWT29262.1"
                     /translation="MNKPAETLTAVPCPACGLLCDDLTVSVQDTQLHVTDKGCAKSHA
                     FFEMPLAETPPHIDGKPASLSDAIAAVVDRLREARHPLIGGLATDITGMRTIMNLAGT
                     ARATLEHMNSYSSMRNLLVMQNSGWQTTTLTEVRNRVDLLVIVGTDIVSSFPRFFERM
                     VWNDETLFGQDTATREVVYLGGRDLDTRAGVSPKGKLPEVLPCDLARLPEVLAALRAI
                     AAGKPVKADAIAGIPAAALVSLADRLKAAKYGVIAWSASALDFPHAELAIQSMTGLVV
                     DINQTTRCAGLPLSGNAG"
BASE COUNT          430 a          694 c          576 g          371 t
ORIGIN      
        1 gaatatttcg acggcttctc catcggctcc aatgacctca cgcaatagtc gctgggtatg
       61 gaacgtgatt ccggtctcat ggcccatggg ttcgatgagc gcgaccctgc ggtcacggtg
      121 ctgctcaaca tggcgatcag tacggccaac cggctcgaca agtacgtcgg tatctgcggt
      181 cagggtccgt cggatcatcc ggacttcgcc gaatggctgg tcaaggctgg catcaagtcg
      241 gtatcgctca atccggatac cgtggtcaag acctggcaac gcctggccaa gctgagctaa
      301 cggacccgac cctaacctat gcatcaccgt acggtattct tcatttcgga cggcactggc
      361 ctgaccgcgc aagcgctcgg ccagctgctg gcgcattttc caaacacgga attccgccag
      421 atccgcctgc cgttcaccaa cacgttgcag caggtgtacg aggccatggc gcgcatcgac
      481 atggcgcgcg ccgaagacaa cgtgccgccg gtggtggtca tgtcgctcgg caatgtcgac
      541 caccgcagcc tgatcaagca gtgcaccgcg tattacatcg acctgttcga gtccttcatc
      601 aatccgctgg gcatcgaact caacgagacg ccgctgacac gcgccggcat cgcccatagc
      661 atccagggcc agaactacca tgaacgcatg gaagccatca atttcacgct cggccatgac
      721 gacggcatga cggaagaagg cctggaagaa gcccaggtca tcctcatcgg cgtctcccgc
      781 tgcggcaaga cgccgaccag tctctacatg gccatgcaat tcggtatcaa ggcagccaac
      841 tatcccctga tcccggaaga cctggagcgc ggccgtatcc cgtccgtcct ggagaatttc
      901 ccgcacaagc tgttcggcat gaccatcaag cctgaacggc tgcattcggt gcgtagcgaa
      961 cgtcgcccca acagtttcta tgcctcgctc gaaaactgcc gcaacgaagt gcgcatggca
     1021 gaagagatga tgcgtaacag cggtgtgcac tggatcgatt ccaccagtcg ctcgatcgag
     1081 gagttgtcga ccaccatcct gcaagagctg aagctgtcca actgaagccg tccgagatcc
     1141 accctcaatc cccggaagtc ctggcctcgc caggtgttaa aatgtcgtca tgaacaaacc
     1201 cgctgaaacg ctgaccgccg ttccctgccc tgcctgcggc ctgctgtgcg atgaccttac
     1261 ggtcagcgta caagacacgc aactccacgt caccgacaaa ggctgcgcca agagccacgc
     1321 cttcttcgaa atgccactgg cagagacccc gccccatatc gatggcaagc ctgccagctt
     1381 gtcagatgcg atcgccgccg tagttgatcg cctgcgcgag gcccgccacc cgctcatcgg
     1441 tggcctcgcc accgacatca ccggcatgcg caccatcatg aacctcgccg gcacagcgcg
     1501 cgcgacactg gagcacatga acagctacag ctcgatgcgc aatctgctgg tcatgcagaa
     1561 ttccggctgg cagacgacga cgctgaccga agtgcgcaac cgcgtcgacc tgctggtgat
     1621 cgtcggcacc gatatcgtca gcagtttccc gcgcttcttc gaacgtatgg tctggaacga
     1681 tgaaaccctc ttcggccagg atacggccac gcgcgaggtg gtctacctag gtggccgcga
     1741 cctcgatacg cgcgccggcg tctcgcccaa gggcaagtta cccgaggtgc tgccttgcga
     1801 cctggcgcgc ctaccggaag tgctggcggc gttgcgggcc atcgcggccg gcaagccagt
     1861 gaaagccgac gccatcgccg gcatccccgc ggcagccctc gtctcgctgg cagaccgtct
     1921 caaggccgct aaatacggtg tcatcgcatg gtccgccagc gcgctggatt tcccgcatgc
     1981 cgaactcgcc attcagagca tgaccggcct ggtcgtcgac atcaaccaga ccacacgttg
     2041 cgcaggcttg cccctgagcg gcaacgcagg t
//
LOCUS       DATMTW010000362         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_635347, whole genome shotgun sequence.
ACCESSION   DATMTW010000362 DATMTW010000000
VERSION     DATMTW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635347"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..976)
                     /gene="rfaP"
                     /locus_tag="VN084_07105"
     CDS             complement(143..976)
                     /gene="rfaP"
                     /locus_tag="VN084_07105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HWT29263.1"
                     /translation="MGLACKSLVLPAEMRANFGDAAAFERIMALEGDVFRDVRGRRTL
                     RFDLQGRSYFAKLHYGVGWGEILKNLLTLRLPIVSAATEWRAIHRLNEIGILTTPAVA
                     YGCEGASPAALRSFIITEDLGDIVSLETLCADWARHPPEASFKRRLIVAVAELARKLH
                     DNGLNHRDFYLCHLCLDQPLLRQGQIRLYLIDLHRVGIRARIRPADRMKDIAALYFSA
                     MDCGLGSRDILRFIRHYRPQGLRKSWLGERPFWRAVGVRAQALYDKFQRHRQRAKIQP
                     G"
     gene            complement(960..2114)
                     /locus_tag="VN084_07110"
     CDS             complement(960..2114)
                     /locus_tag="VN084_07110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWT29264.1"
                     /translation="MRFAFLIFKYFPYGGVQRDMLRIANALALAGHNVDIFTMSWEGE
                     APQNGIRLRLIDARGMINHKRYDNFIRQAREEIAKVNYSLVVGFNRMPGLDVYFAADP
                     CFAERAQNQRGFFYRLSGRYRWFSKCERAVFAPEANCQVLLLSEREKADFQRWYRTPE
                     HRFHLLPPYLSQARMALHDKQAMRTRLREAFGFDEEDRVLLLVGSGFRTKGLDRAIDA
                     LAALPGKQRVKTRLLAVGQDNPKAFLRMAADKRLSKRVQILPGRNDIPQLMQGADLLI
                     HPARNELAGHVLLEAMASGLPVLVTDVCGYAFHIARAQAGVVLASPFEQQALNHALAS
                     MLDSEHLPTWAAQGIAYARAIMANNQGEAEANILTDIVRNKWGGADGPGL"
     gene            2276..3244
                     /gene="waaC"
                     /locus_tag="VN084_07115"
     CDS             2276..3244
                     /gene="waaC"
                     /locus_tag="VN084_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HWT29265.1"
                     /translation="MPRLLIVKTSSLGDVVHNLPIVEDIRAQHPDMVIDWLVEEAFAD
                     IPALHPAIDRVIPVAIRRWRKQLFQGSTWQEIGAMRAQIKRHDYDLVLDTQGLLKSAL
                     LCCLATRTRHGQDRRSAREPLAACFYQRRHASAKGQHAVIRNRALAAAAFGYAMPEAL
                     PDYGLRTVASRPSASDGMSYVVGLHATSRDSKLWPVDRWIRLGRHLASLELSLLLPWG
                     NETERQRAQQIAEAVPHASVLPRMSLHELAAVLAGARAAIGVDTGLVHLAVALGLPTI
                     ALYTDTDPRLTGIYPGAGGQAINLGGQDAAPDTELVIATVNDLLST"
     gene            3329..3859
                     /gene="grpE"
                     /locus_tag="VN084_07120"
     CDS             3329..3859
                     /gene="grpE"
                     /locus_tag="VN084_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWT29266.1"
                     /translation="MQQENQHPESTEETSTESLQDGAAGSLDDRISELENQLSEAQAA
                     VLYAKAEGENIRRRAHDDIDKARKFALEKFAGELLAVKDSLDAALHVENATVESYKSG
                     VELTVKQLSSVFDKFSIGEVNPVGEKFDPNKHQAISTVDSDQEPNTVVSVLQKGYTLN
                     ERVLRPALVMVAKAKS"
     gene            3966..>4964
                     /locus_tag="VN084_07125"
     CDS             3966..>4964
                     /locus_tag="VN084_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWT29267.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGGKPRVIENAEGARTTPSIIAYQD
                     DGEILAGAPAKRQAVTNPKNTLYAVKRLIGRRFEEKEVQKDIGLMPYTISKADNGDAW
                     VEVRGEKMAPPQISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKTGKGDRKIAVYDLGGGTFDISIIEIADVDGEMQFE
                     VLSTNGDTFLGGEDFDQRLIDYIIEEFKKINGLDLSKDAIALQRTKASAERVKIELSS
                     SQQTEINEPYIAMANGAPVHLNLKITRAKLESLVEDLIAKSIEPCRTAIKDAGIKVS"
BASE COUNT         1099 a         1540 c         1453 g          872 t
ORIGIN      
        1 ctgccggagg aactccagcg cgcttctagt aagctctagc gtcttgtaat tcaccatcac
       61 gatgctgaaa tcgcccaaag cgtcatctgt cgctgaggta ttcgggttat gcatgtaacg
      121 cgtcttcctg gggttcgttc atttaaccgg gctgtatttt cgcacgttgc ctgtgccgct
      181 ggaacttatc atacaaagcc tgcgccctga caccgaccgc tcgccagaac gggcgttcgc
      241 ccaaccacga cttgcgcaga ccctgcgggc ggtaatgccg gatgaaccgc aggatatcgc
      301 gcgagcccag gccgcaatcc atggccgaga aatagagggc cgcgatatct ttcatgcggt
      361 cggcaggccg gatgcgggca cggatgccaa cgcgatgcag gtcgatcaga tacagcctga
      421 tctgtccctg ccgtagcagc ggctgatcca ggcacagatg acacagatag aaatcacgat
      481 gattgaggcc gttatcgtgc agcttgcggg caagttcggc gacggcgacg ataagtcttc
      541 gcttgaagct tgcctctggg ggatgccgtg cccaatctgc gcatagcgtt tcgagcgaga
      601 cgatatcacc caggtcttcg gtgatgatga aagagcgcaa cgcggccggg ctcgcgcctt
      661 cgcaaccgta agccacggct ggcgttgtca ggataccgat ctcgttgaga cgatggatgg
      721 cgcgccactc ggtggcggcg ctgacgatgg gcaggcgcaa cgtcagcagg ttcttgagga
      781 tctcgcccca gcccacgcca tagtgcaatt tggcgaaata ggaccggccc tgcaggtcga
      841 atcgcagcgt acggcggccg cgcacgtcgc ggaacacgtc gccttccagt gccatgatgc
      901 gttcgaacgc agcagcgtca ccaaagttgg cacgcatctc cgcgggcagc accagcgact
      961 tacaggccag gcccatcggc acctccccat ttgttgcgca cgatgtccgt caggatattg
     1021 gcctcggcct cgccctggtt gttagccatg atcgcacggg cataggcgat accttgggcc
     1081 gcccaggtcg ggagatgctc ggagtccagc atgctggcca acgcatggtt cagcgcctgc
     1141 tgctcgaagg gtgacgccag cacgacgccc gcttgcgcgc gggcgatatg gaacgcataa
     1201 ccgcagacat cggtgaccag cacaggcaag ccgctggcca tcgcttccag cagcacatgg
     1261 ccggcgagtt cgttgcgcgc gggatggatc agcaggtcgg cgccctgcat cagttgcggg
     1321 atatcgttac gacccggcaa tatctgcaca cgcttgctaa gccgtttatc ggccgccatg
     1381 cgcaggaacg ccttggggtt atcctggccg accgccagca ggcgggtctt cacccgctgt
     1441 ttgcccggca aggccgccag ggcgtcgata gcacgatcca gccccttggt gcggaacccg
     1501 gaacccacca gcaacaacac ccgatcttct tcatcgaaac cgaacgcctc acgcaacctg
     1561 gtacgcatcg cctgtttgtc atgcagggcc atgcgcgcct gcgagagata aggcggcaag
     1621 agatggaaac gatgttctgg cgtgcgatac cagcgctgga aatccgcctt ctctctctca
     1681 gagagcagca gcacctggca attggcttcg ggcgcgaaga ccgcacgttc acacttggag
     1741 aaccagcgat atcgtccgga aagtcgatag aagaaaccac gctggttctg cgcgcgctcc
     1801 gcgaagcagg gatctgcggc gaaatacaca tcgaggccgg gcatgcgatt gaagcctacg
     1861 acaaggctgt aattgacctt ggcgatctct tcccgcgcct ggcggatgaa attgtcatag
     1921 cgcttgtggt tgatcatgcc gcgggcatcg atcaggcgca gacggatgcc gttctgcggc
     1981 gcctcgcctt cccaggacat ggtgaagata tccacattat gacccgccag cgccaaggca
     2041 ttggcgatgc gcagcatgtc gcgctggact ccgccgtagg ggaaatactt gaagataagg
     2101 aatgcgaatc gcaattcgga acgctggcta tgaaagattt aattgtatgc gaaaataggc
     2161 ccaagcccct gattaagtcc cgcatgaacc ccatcaccga caaaaacaag atgtcccacg
     2221 aacgcctgac caggcccctc gccagcagca tccccagcat gacaagagac tgcagatgcc
     2281 gcgcctgctg atcgtcaaaa cgtcttcgct gggcgatgtg gtacataacc tgccgatcgt
     2341 cgaggatatc cgcgcgcagc atccggacat ggtcatcgat tggctggtgg aggaagcctt
     2401 cgccgacatc ccggccttac atccggcgat cgaccgcgtc atcccggtag cgatccgccg
     2461 ctggcgcaag cagctgtttc agggcagtac atggcaggag atcggggcga tgcgcgcaca
     2521 aatcaagcgc catgattatg atctcgtgct ggatacgcaa gggctgttaa agagcgcact
     2581 gctgtgctgt ttggccacta ggacccgtca cggccaggat cgccgctctg cacgcgaacc
     2641 cctcgccgcc tgcttctacc agcgacgcca tgcgagcgcc aagggccagc acgccgtcat
     2701 ccgcaaccgg gcactagcgg ccgccgcgtt cggatatgcg atgccggaag cgttgccgga
     2761 ctatggcttg cgcaccgtcg cctcccgacc gtccgccagc gacggcatgt cctatgtcgt
     2821 cggcctgcac gccaccagcc gcgacagcaa attatggcct gtggatcgct ggatacgcct
     2881 tggacggcac ctggcctcac tggaactgtc attgctgctg ccctggggca acgagaccga
     2941 acgccagcgt gcgcaacaga tcgccgaagc agtgccgcat gcctccgtgc tgccgcgcat
     3001 gtcgttacac gaactggccg cggtgctggc tggggcgcgc gccgcgatcg gcgtcgatac
     3061 cgggctggtg cacctcgctg tggcgctggg cctgcccacg atcgcgctct acaccgacac
     3121 cgaccctagg ctcaccggca tctacccggg tgccggtggc caggccatca acctcggtgg
     3181 gcaagacgcc gcgcccgata ccgagctcgt catcgccacc gtcaatgacc tgctgagcac
     3241 ctgagagaaa aatcgcgccg tgcacttgaa atcgaaaagc ccatccgcat ttaagggttt
     3301 attacggtct tttttcagag ggaaacccat gcagcaagag aatcaacatc cagaatccac
     3361 agaagagaca tccaccgaat ccctgcagga cggtgctgcc ggctcgctcg atgaccgtat
     3421 cagcgaactg gagaaccagt tgtccgaagc gcaagccgcc gtactgtatg ccaaggccga
     3481 gggcgagaac atccgtcgcc gtgcacatga cgatatcgac aaggcacgca agttcgcgct
     3541 ggagaagttc gccggcgagc tgctcgccgt gaaagacagt ctcgatgcgg cgctccatgt
     3601 ggagaacgcg accgtcgaga gctacaagag cggcgtcgaa ctgacggtca agcaactgtc
     3661 cagcgtattc gacaaattca gcatcggcga agtcaatccc gtgggcgaga agttcgatcc
     3721 caacaagcac caggccatca gcaccgtcga ttcagaccag gaacccaata ccgtggtcag
     3781 tgtgctgcag aaaggctaca cgctgaatga acgcgtgctg cgccctgccc tggtgatggt
     3841 cgccaaggcc aaatcctagt tcaaacgatt tacttacata cagtgggctt gaattcgctc
     3901 aggcaatccc cacttttgca acatcggtat taatttttca aagtattcgc acaggaagac
     3961 acatcatggc aaagattatc ggtattgatt tgggcaccac caactcctgc gttgcggtca
     4021 tggaaggcgg caagccgcgc gtgatcgaaa acgccgaagg cgcccgcacc accccatcca
     4081 tcatcgccta tcaggacgac ggcgagatcc tggctggcgc acccgccaaa cggcaggcag
     4141 tgaccaaccc caagaacacc ctctacgcgg tgaagcgcct gatcggtcgc cgctttgaag
     4201 agaaggaagt ccagaaggac atcggcctca tgccctacac catcagcaag gccgacaacg
     4261 gcgatgcctg ggtggaagtg cgcggcgaga agatggcgcc tccacagatc tccgcagaag
     4321 tcctgcgcaa gatgaagaag accgctgaag attacctggg cgaagaagtg accgaagcgg
     4381 tcatcacggt gccggcctac ttcaacgact cgcagcgcca ggcaacgaaa gacgccggcc
     4441 gcatcgccgg tctcgacgtc aagcgcatca tcaacgaacc gaccgcggct gcactggcgt
     4501 tcggcctgga caagaccggc aagggcgacc gcaagatcgc cgtgtatgac ctcggcggcg
     4561 gcaccttcga catttcgatc atcgagatcg cggacgtcga cggcgagatg cagttcgaag
     4621 tgctgtcgac caacggcgat accttcctcg gcggcgaaga cttcgaccag cgcctgatcg
     4681 actacatcat cgaggaattc aagaagatca acggcctcga cctgtcgaag gacgcgatcg
     4741 cgctgcagcg taccaaggca tcggcagaac gcgtcaagat cgaactgtcg tcgtcgcaac
     4801 agaccgaaat caacgagccg tatatcgcga tggcgaacgg cgcgccggtc cacctgaacc
     4861 tgaagatcac gcgcgccaag ctggaatcgc tggtggaaga cctgatcgcc aagtcgatcg
     4921 agccttgccg caccgcgatc aaggatgccg gcatcaaggt atcc
//
LOCUS       DATMTW010000363         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_31228, whole genome shotgun sequence.
ACCESSION   DATMTW010000363 DATMTW010000000
VERSION     DATMTW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31228"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VN084_07130"
     CDS             <1..1077
                     /locus_tag="VN084_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWT29268.1"
                     /translation="VELQDNHPERHVVVKGDTLWGISAKFLKDPWQWPKVWRMNREQI
                     KNPHRIYPGDVVVLDMSSGDPQLRLLSENTVMLEPGIREEPLEKAAIPTIQPNVISPF
                     LTQPLVINNDELNAAPMIVSGPENRLVLAPGTNIYVSRIDEADGRQWHIYRPGKVLID
                     PDSKAVLGTEAVYLGDAQVKKFGEPATVEVVRAKEEIFKQDRLVPAPETLQNSFIPRA
                     PEQEVLGRILSVHGGVAEAGANSIVSINLGTAEGMEEGHVLAIKRAGKLVQMPSGNRR
                     EYKPYRWPANKNKPYDWSTGAYASATESSQPAAGENTDPSLIKLPSERIGLLMIFRTF
                     EHVSYALVMQASDSINVLDLVETP"
     gene            1090..>2024
                     /gene="dprA"
                     /locus_tag="VN084_07135"
     CDS             1090..>2024
                     /gene="dprA"
                     /locus_tag="VN084_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWT29269.1"
                     /translation="MAIVDAEDAKRWLALSLIPGLRSQGLCKLLQAFGGPAAIFATPI
                     QRLKEITNPEIARLIAHGPDAERLQPAVDWLSQPHNHLITLSDREYPQTLLEIPDPPP
                     VLYAKGDLTLLNRPALAMVGSRNATPQGEKNSEDFAAALCDAGLCIVSGLALGIDGAA
                     HRGALRANGATIAVVGTGLDIVYPARHRDLAHQIVERGLIISEFPIGTPSRAQNFPRR
                     NRIISGLSLGTLVVEANVQSGSLITAKLAAEQGREVFAIPGSIHSPVSKGCHQLIKQG
                     AKLVDNIQDIVDELAIFTTSSSPAGSTKTTPEAHPV"
BASE COUNT          456 a          623 c          579 g          366 t
ORIGIN      
        1 gtcgagcttc aggataacca ccccgagcgt catgtggtcg tcaagggcga caccctctgg
       61 ggcatctccg ccaaattcct caaggatcca tggcaatggc ccaaggtctg gcgcatgaac
      121 cgcgaacaga tcaaaaatcc ccatcgcatc tatcccggcg atgtcgtcgt gctggacatg
      181 agttcgggcg atccgcaatt gcggctactg agcgagaaca ccgtcatgct ggagccaggc
      241 attcgcgaag agccgctcga gaaagcggcg atccccacca tccagccgaa tgtcatctcg
      301 cctttcctca cacagccgct ggtcatcaat aacgacgaac tcaatgccgc ccccatgatc
      361 gtcagtgggc cagagaaccg gctggtgttg gcgccaggta ctaatatcta tgtcagcagg
      421 atcgatgaag ccgacggtcg ccagtggcat atctaccgcc cgggcaaggt gctgatcgat
      481 ccggacagta aagcggtgct cggcactgaa gccgtctatc tcggcgacgc gcaggtgaag
      541 aagttcggcg aaccggctac ggtggaggtg gttcgggcca aggaagagat cttcaagcag
      601 gacagactag tgccagcacc ggagaccctg cagaacagct tcatcccgcg tgcgccggag
      661 caagaagtcc tcggccgcat cctgtcggtg cacggtggcg tcgcagaggc cggcgcgaat
      721 tcgatcgtca gcatcaacct gggaacggcg gaaggcatgg aagaaggcca tgtgcttgcg
      781 atcaagcgcg caggcaaact ggtgcagatg ccctccggca atcgtcgcga gtacaagcct
      841 taccgttggc cagcaaacaa gaacaaacct tatgactgga gcacaggcgc ctacgccagc
      901 gcgactgaaa gctcgcaacc tgcagcaggc gagaataccg acccttcgct catcaaattg
      961 ccgagcgaac gtatcgggct gctgatgatc ttccgtactt tcgagcacgt ctcatacgcc
     1021 ctggtgatgc aggcaagcga ttcgatcaac gtactcgatc tggttgagac cccataagcc
     1081 atcgcgcaga tggcgatcgt tgacgcggaa gatgcgaagc gctggctagc gctaagcctg
     1141 attccagggc ttaggagcca aggcctgtgc aagctgcttc aggccttcgg cgggccggca
     1201 gcgatcttcg ccacacccat ccaacggctc aaggaaatca ccaacccgga gatcgcaagg
     1261 ctcatcgcgc atggtccgga tgccgaacgg ctacagcctg ctgtcgattg gctgtcgcag
     1321 ccgcataatc acctaatcac gttatcggac agggaatatc cgcagacctt gttggagatc
     1381 ccggatccgc caccggtcct ctatgccaaa ggcgacctga ccctgctcaa tcgaccggcg
     1441 ctcgccatgg tgggcagtcg caatgccaca ccacaaggcg agaagaattc ggaggacttc
     1501 gccgccgcgc tctgtgatgc cggcctctgc atcgtcagcg gtcttgcgct cggaatcgac
     1561 ggcgctgctc atcgcggcgc cttgcgcgcc aatggcgcaa ccatcgctgt cgttggtact
     1621 gggctcgata tcgtctaccc ggcccggcat cgtgatctcg cccatcagat cgtcgaacgt
     1681 ggcctgatca tctctgaatt cccgatcggc acgccatcgc gtgcgcagaa cttcccgcga
     1741 cgcaaccgca tcatcagcgg actgtcgctg ggcacgctgg tggtggaagc caatgtccag
     1801 agcggctccc tcatcacggc aaaactggcg gctgagcagg ggcgagaggt gttcgccatt
     1861 ccgggctcca tccattcacc ggtatccaaa ggttgtcacc agctcatcaa gcagggcgcg
     1921 aaactggtcg acaacataca agatatagtg gatgaattag ctatttttac aacaagtagt
     1981 agtcctgccg gcagtaccaa aaccacgcca gaagcccatc cggt
//
LOCUS       DATMTW010000364         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_635376, whole genome shotgun sequence.
ACCESSION   DATMTW010000364 DATMTW010000000
VERSION     DATMTW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635376"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VN084_07140"
     CDS             <1..788
                     /locus_tag="VN084_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWT29270.1"
                     /translation="TLFERSRTDISMTPIGEQIVEQATRVLEEANRIKEIARQGNDQL
                     SGPIKLGVIYSAGPYLLPEIIPILRRTAPDMPLDVEENLTHVLETQLRNGVIDAAIVA
                     LPFDIPGIQVQPLYDESFVVVVPADHSWAARRSIHPDELAEQKVLLLNSGHCFSNQLI
                     QACPDLTRKGEVLQGNSLETIRNMVASNLGITVLPCSATVERYQNPLIKAIPFQQPIP
                     SRRMALAWRKSYGREKAIECLVEAIRQIDSDCMKPVKQNKKEA"
     gene            complement(818..889)
                     /gene="pqqA"
                     /locus_tag="VN084_07145"
     CDS             complement(818..889)
                     /gene="pqqA"
                     /locus_tag="VN084_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HWT29271.1"
                     /translation="MWTKPAATEMRFGFEVTMYVCNR"
     gene            complement(1056..1727)
                     /locus_tag="VN084_07150"
     CDS             complement(1056..1727)
                     /locus_tag="VN084_07150"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HWT29272.1"
                     /translation="MDRLGEVDATTTSEVAQPAPQPAPLTAAAPDVAPARLTPSPVAK
                     PAETPGIFGEVKSKKRIAGRRKITWPYYVFALLLLMTGIVQVLYFMRTPLSTQWPELK
                     PFLVSTCSFVGCKVDLPRNIDQLVIDDSDLQEDAEHEDVIRLSTLLINNAPYAQAYPL
                     LELTLTDRNDRPVLRRAFTAAEYLNKGRDVNAGIAPGEELQIKLALTSSDVDVSGYRV
                     YLSYP"
     gene            complement(1841..2728)
                     /gene="prmA"
                     /locus_tag="VN084_07155"
     CDS             complement(1841..2728)
                     /gene="prmA"
                     /locus_tag="VN084_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWT29273.1"
                     /translation="MSWVSLNIEASDSSADVLTEALMSLGALSASIEDANAETVDEQP
                     IFGEPGDPPPGIWRQNVVSALFDADTDVAAIMAALAEATGYADLRYTTEHIAEQDWVR
                     ATQSQFDPIRIRDDLWIVPSWHDAPNPAGLNIVLDPGLAFGTGSHPTTHLCLSWLADT
                     IKGEEQVLDYGCGSGILAIAAKKLGAQRVIGVDIDGQAIQSSCYNAERNDVLAEFFMA
                     DRFSGTGYDIVVANILSSALSVLAPALAAACRQQGRIALSGILLEQASQVSGIYDEWF
                     DMDAPVTMESWVLLTGRKR"
     gene            complement(2745..>3321)
                     /locus_tag="VN084_07160"
     CDS             complement(2745..>3321)
                     /locus_tag="VN084_07160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146762.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWT29274.1"
                     /translation="IVAKAMADMGYRGAGTIEFLWENGEFYFIEMNTRLQVEHPVTEM
                     ITGIDIVQQQIRIAAGEKLPFRQKDIQFHGHAIECRINAEDPFNFVPSPGRISTFHMP
                     GGPGIRVDTHAYQNYLVPPHYDSMICKLIAYGDTREQAIAKMRIALSEMVVEGIKTNV
                     PLHSELMADAAFHQGGTSIHYLEQKLGISSK"
BASE COUNT          730 a          914 c          975 g          702 t
ORIGIN      
        1 ttacactctt cgagcgcagc cgtaccgata tcagcatgac accgatcggc gagcagatcg
       61 tcgagcaagc gacgcgagtg ttggaagagg caaaccggat caaggagatc gcccggcagg
      121 gcaatgatca gcttagtgga ccgataaaac tcggggtcat ttattccgca gggccttacc
      181 tgttgcccga gatcattccc atcctgcggc gtaccgcccc ggatatgccg ctggatgtgg
      241 aagagaatct gactcatgtg ctggagacgc agctgcgtaa cggcgtgatc gacgccgcca
      301 tcgtggcctt gcctttcgat atcccgggta tccaggtcca gcccttgtat gacgaatcct
      361 ttgtcgtcgt ggtcccagcc gaccattcct gggcggcgcg caggtctatc cacccggatg
      421 agctggccga acaaaaggtc ttgctgttga acagcggcca ttgtttcagc aatcagctga
      481 ttcaggcatg tccggatctg actcgcaaag gggaggtgtt gcagggtaac tcactggaaa
      541 ccatcaggaa catggtggcg tccaacttgg ggataacggt attaccgtgc agcgcgacgg
      601 tggaacgtta ccagaacccc ttgatcaagg cgatcccgtt ccagcagcca atcccgtcgc
      661 gcaggatggc gttggcatgg cgcaagagtt atggacggga gaaagcgatc gaatgtctgg
      721 tggaagcgat ccggcagatc gattcggatt gcatgaagcc ggtgaagcaa aacaaaaagg
      781 aggcctaggc ctcccttttt agtctatctg caatcgctta gcggttgcaa acgtacatgg
      841 taacttcgaa accgaaacgc atttcagtag cagctggctt ggtccacatg gcgttatctc
      901 ctaaatgttt attgatcgag accaacgaag tcgacctctg atggacacat agtacagaag
      961 cgcatggcaa gccgcgtccg catactcatg aatacggcct aatgaaaatc atgagatggc
     1021 atgctacgaa tcacacatga aagagcaagg acaggctaag ggtagcttag atagacccgg
     1081 tagccggaga catcgacatc gctggaagtc aaggccagct tgatttgtag ctcttcccca
     1141 ggcgcaatgc cagcgttcac gtcgcgaccc ttattcagat attcggcggc cgtgaaagcg
     1201 cgccgcagca ccgggcggtc gttcctgtcg gtaagcgtga gctcaagtaa aggataagcc
     1261 tgcgcgtacg gcgcattgtt gatgagcagt gtgctcaatc gaatgacgtc ctcgtgctcc
     1321 gcatcttcct gtagatccga atcatcgatc accagctgat cgatattacg tggcaaatcg
     1381 accttgcagc cgacgaagga gcacgtagaa acgaggaaag gcttgagttc aggccactgc
     1441 gtcgacaagg gcgtccgcat gaaatacaac acctgcacaa tcccggtcat caataacagt
     1501 agcgcgaaca catagtaggg ccaggtgatc ttgcgtctgc ctgcaatacg ctttttggat
     1561 ttcacctcac cgaagatgcc gggcgtctcg gccggcttcg ccacaggcga tggtgtaagc
     1621 ctggcgggag cgacgtcggg tgcggcggcg gttagtggtg ctggctgcgg ggccggctgc
     1681 gctacttcgc tggttgtcgt tgcatcgact tcgcccaggc ggtccatggc attgaataca
     1741 tggctgcatt taccgcagcg aacatcacca tgatgcgcgt tgagttgtgc cgtgttgacg
     1801 aagaaatgag tcgaacaggc gggacaggcg gtgaccaggc tcatcgttta cggcccgtga
     1861 gcagtaccca gctttccatg gtgacggggg catccatatc gaaccattcg tcatagatgc
     1921 ctgagacttg agatgcctgt tcgaggagga taccggatag ggcgatacgt ccctgttgcc
     1981 tgcacgcagc agcgagcgca ggtgccagga cggaaagcgc gctggagagg atattcgcca
     2041 caacgatatc gtaccctgta ccggaaaaac ggtctgccat gaaaaattcc gctaagacgt
     2101 catttctctc tgcattgtag cagctcgatt gaatcgcctg accgtcgatg tcgacgccga
     2161 tgacacgctg cgcgcccagc ttcttcgctg cgatcgcgag gatgccggag ccgcaaccat
     2221 aatcgagtac ctgttcttca cctttgatcg tgtccgcgag ccagctcagg caaaggtggg
     2281 tggtcggatg gctaccagta ccgaaggcca gtccgggatc gagcacgata ttgaggccag
     2341 cggggttggg cgcatcgtgc caggacggca cgatccatag atcgtcgcgg atccggatag
     2401 gatcgaactg ggattgcgtg gcacgcaccc aatcctgttc ggcgatgtgc tcggtcgtgt
     2461 agcgcaggtc tgcatagcct gttgcttccg ccagagccgc catgatcgcg gcgacgtcgg
     2521 tgtctgcgtc gaacagagcg ctcaccacgt tttgacgcca gatgcctggt ggcggatcgc
     2581 cgggctcgcc aaagatcggc tgttcgtcga ccgtctctgc attggcatcc tcgatgcttg
     2641 cagacaaggc cccaagactc atcaacgcct cggtcaatac gtcggcgctg ctgtcgctgg
     2701 cttcgatgtt cagcgagacc caactcatgg taaaccccgc ttcattactt ggacgagata
     2761 cccagcttct gctccagata atggatgctc gtgccgccct gatggaaggc agcatcggcc
     2821 atcagctcgc tgtgtagcgg cacgttggtc ttgataccct cgaccaccat ctcggacagc
     2881 gcgatccgca tcttggcgat cgcctgctcg cgcgtatcgc cataagcgat gagcttacag
     2941 atcatggaat cgtaatgcgg cggcaccagg taattctggt aagcgtgggt atcgacgcgg
     3001 atgccaggtc caccaggcat gtggaaggta ctgatgcggc ccggtgacgg cacgaaattg
     3061 aatgggtcct cggcattgat acggcattcg atggcgtggc catggaactg gatatccttt
     3121 tggcggaaag gcaacttctc gccagccgcg atgcggatct gttgctgcac gatatcgata
     3181 cccgtgatca tctcggtcac cgggtgttcg acctgcagtc gcgtgttcat ctcgatgaag
     3241 tagaactcgc cgttttccca caggaattcg atcgtgcccg caccgcgata gcccatatcg
     3301 gccatcgcct tggcgacgat c
//
LOCUS       DATMTW010000365         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_534603, whole genome shotgun sequence.
ACCESSION   DATMTW010000365 DATMTW010000000
VERSION     DATMTW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534603"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1216)
                     /locus_tag="VN084_07165"
     CDS             complement(77..1216)
                     /locus_tag="VN084_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWT29275.1"
                     /translation="MKCGDYSYIWQAPDWPNWRYDLAALASPLAQVSQAQGLLLGRLT
                     DIGLGLRDQASLAALTDDVIKTSEIEGEHLNVASVRSSIARRLGVDIGSLAPVDRHVE
                     GVVEMVLDATINSASPLTQTRLFAWHAALFPTGYSGLSKIAIGRWRDDAQGPMQVVSG
                     PIGRQRVHYEAPPAEYLSNEVERFLEWLNAPLQVEPALIRAGLGHLWFVTLHPFDDGN
                     GRIARAIGDLLLARADGSPQRFYSLSAQIQRERKAYYEILERTQKGNLDVTEWLLWFL
                     QMLGEALRLANHALDAVLDKARFWQRLSGIALNERQVKVLNRLLDGFEGKFTSSKWAA
                     LAKCSPDTALRDIQELVTQGVLQRSGAGGRSTSYDLVKKGPNPPP"
     gene            complement(1543..>2430)
                     /locus_tag="VN084_07170"
     CDS             complement(1543..>2430)
                     /locus_tag="VN084_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWT29276.1"
                     /translation="ADLAQRHEQAAKRVAAQLAKLVPADQPTPYMVAKGLGPQPGTFT
                     DKDGKKTCLPAIDANGKLWTMQYVGEDGTKRFAKDSRKEGCFHVIGQGLDELANVPAI
                     VIAEGYATAATLRQTLGYATVSAFDSGNLAPVAQALHQKYPDKPVVVACDDDRHLELT
                     QGVNPGRSKGEEAARLVGGIALLPIFAPGEHAYPAGLDPVTPELFRKHQRNGATLSEE
                     QLAALDRMKQFTDFNDLANRSVLGKDGIERQVKAACNTSVANGASVEQQEVQRIRQLA
                     QDTAQEQKPKRRRKAATIA"
BASE COUNT          439 a          747 c          728 g          516 t
ORIGIN      
        1 atgttcgctc tcgacttccg gcaagatctg ctggaaggtg tcgcgtgttc cgtctccgcc
       61 gctgtccaac cagcagttag ggcggaggat tcggcccctt ctttaccaag tcatagctgg
      121 tactgcgccc accggctcca gaacgctgca ggactccctg agttaccagt tcctggatgt
      181 cccgtagcgc ggtatcgggc gagcatttgg ccagcgcggc ccatttgcta ctggtgaatt
      241 tgccttcgaa accatcgagc aggcggttga ggacctttac ctgacgttcg ttaagcgcga
      301 ttcccgacag gcgctgccag aaacgggcct tgtccagcac cgcatcgaga gcgtgattgg
      361 ccagccgcag ggcctcgcca agcatctgca ggaaccacag gagccactcc gtcacatcca
      421 ggttcccctt ctgggtgcgc tccaggatct cgtaataggc tttgcgttca cgctgtatct
      481 gcgccgacag gctgtagaag cgctggggac tgccatccgc ccgcgccagc aaaagatcgc
      541 caatcgcgcg tgcgatgcgg ccgttaccgt cgtcgaacgg atgcagtgtg acgaaccaca
      601 ggtgtcccag tccggcgcgg atcagcgctg gctctacctg gagcggcgca ttgagccatt
      661 ccaggaagcg ctctacttcg ttggacagat actcggcggg tggcgcttcg tagtgtacgc
      721 gctggcggcc aatggggccg gagaccacct gcatcgggcc ttgggcatcg tcgcgccacc
      781 taccgatggc gatcttgctc aggccggaat agccggtcgg gaaaagagca gcatgccagg
      841 caaacagacg tgtctgcgtg agtggcgacg cactgttgat cgtcgcgtcg agcaccattt
      901 cgaccacgcc ttccacgtgc cggtctaccg gggccagaga gccgatatca acgccgagac
      961 gacgcgcgat ggacgagcgt accgatgcaa cgttcagatg ttcgccctcg atttcgctag
     1021 tcttgatgac gtcatcggtc agcgcggcaa ggctggcctg gtctcgcaaa cccaggccaa
     1081 tatcagtgag ccgacccagc aatagcccct gtgcctgact gacctgcgca agaggcgatg
     1141 ccagtgccgc cagatcgtag cgccagttcg gccaatcagg agcttgccag atataagaat
     1201 aatctccgca tttcatgcgg tgattgtaag tcgtaatcgc cgcatgagca atttaaacaa
     1261 cgcagaaatt gcggtgattg ctccagtatt ccccgcagtg actttcgcta tctctacaac
     1321 ggaacgacaa cggagacgaa gtcctcatga tattggcggt cgactcttgg cggcagggca
     1381 cgttgcagtt agcggtccac gaggcgtcca ttcggaatgc gacaatgcga aggtttcggg
     1441 gctcgctgca ggaccggctt ggcacaacgg tcgaccatgc caatgatgat gacgttgcag
     1501 ctgtccggac cgccctatag agcgcggccc agaaacttag gctcaagcga tcgtcgcggc
     1561 cttccgacgt cgcttaggtt tctgctcctg tgccgtgtct tgtgccagct ggcgaatccg
     1621 ctgcacttcc tgttgttcga cggaggcacc gttagcgact gacgtgttgc acgcagcttt
     1681 tacctggcgc tcgatgccat ccttaccgag cacgctgcgg ttcgcgagat cattgaagtc
     1741 agtgaactgc ttcatgcggt cgagcgcggc cagctgttcc tcgctcagtg tcgcgccgtt
     1801 tcgttgatgc ttgcgaaaca gttcgggtgt tacagggtca agccccgcag ggtaggcgtg
     1861 ctcgccgggt gcgaagatcg ggagcagggc gattccccca accagtcttg cagcttcctc
     1921 gcccttgctg cggcccggat ttacaccttg ggtcagttcg agatgacggt cgtcgtcgca
     1981 tgcgacgacg actggcttgt ccggatactt ctggtgcagc gcctgcgcca ctggcgcgag
     2041 attgccggag tcgaaagccg acacggtcgc atatcccagc gtctggcgta gcgtggcggc
     2101 ggtcgcgtag ccctccgcga tcacgatcgc cggcacgttg gcgagttcat ccaggccttg
     2161 gccgatcacg tgaaagcagc cttccttcct gctgtctttc gcaaaccgtt tcgtaccgtc
     2221 ctcgccgacg tattgcatcg tccatagctt gccgttcgca tcgatcgccg gaaggcaggt
     2281 cttcttgcca tccttgtcgg tgaacgtgcc tggctgcggc ccgagtccct tcgctaccat
     2341 gtacggcgtc ggttggtcgg ccggcaccaa cttggccaac tgggcggcca cccgctttgc
     2401 tgcctgttca tggcgctgcg ccagatctgc
//
LOCUS       DATMTW010000366         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_399877, whole genome shotgun sequence.
ACCESSION   DATMTW010000366 DATMTW010000000
VERSION     DATMTW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399877"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VN084_07175"
     CDS             <1..411
                     /locus_tag="VN084_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29277.1"
                     /translation="SWLGLALVVAFGMDAYALGWKGVSDYCMSPGYWGLIPTYAMVWG
                     AGRWLARREKPFAALPYALFGWAAVSIAFVVSNAFWYAFSDKVATMPVSAFSAAVSAY
                     YLPYAGYSLMYLALAWVAYGVLASGILRPKTSKV"
     gene            408..1013
                     /gene="cobO"
                     /locus_tag="VN084_07180"
     CDS             408..1013
                     /gene="cobO"
                     /locus_tag="VN084_07180"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWT29278.1"
                     /translation="MNETDKTGRHVARMQRKKALVDAHIEAARVSRGLLLIHTGNGKG
                     KSTAAFGLLARALGHGKRAAVIQFIKSRSDTGEEAFFRTHPSVQWHVMGDGFTWETQD
                     AESDRKSGRAAWVQVCRYLQDPAIDLLILDEFTYALKYGWLSLEEVAEALHKRPAMQH
                     VVITGRAAPPELIALADTVSEALPIKHAFSAGVEAMPGIEF"
     gene            1053..>2086
                     /locus_tag="VN084_07185"
     CDS             1053..>2086
                     /locus_tag="VN084_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWT29279.1"
                     /translation="MPSVSCPALLLSAPASGQGKTMTSAALARAWRDRGLRVQVFKCG
                     PDFIDPMILEVASGHPVYNLDLGMCGEADVRERLYRAAQTSDVLLIEGVMGLFDGQPS
                     SADIAIRFGIPVALTIDASAMAQTFGALASGLLGHDPAIRPAGAIANNIGSAGHAEFL
                     HSSLPAHIPWLGALPKDTRYHLPERHLGLHLAHEIADLEARIALAAKTLTANAKLTLP
                     EPVPFHAETLPAMPRLLDGKTIAVARDEAFCFLYPANLDCLQALGARLTFFSPLHDSA
                     LPEAEAVWLPGGYPELHMSTLSANQGMRDSLRQALAGDIPMLAECGGMMALSASIDDR
                     PAFGLLPGKSR"
BASE COUNT          369 a          687 c          624 g          406 t
ORIGIN      
        1 agctggctgg gccttgccct agtggtcgcg ttcggtatgg acgcttacgc gctgggctgg
       61 aaaggcgtat ccgattactg catgtcaccc ggctactggg gcttgatccc gacctacgcg
      121 atggtctggg gcgccggccg ctggctggcc agacgcgaga agcctttcgc cgcgctgccc
      181 tatgccttgt tcggctgggc tgccgtcagc atcgctttcg tcgtctcgaa tgccttctgg
      241 tacgcgttct ctgacaaggt cgccaccatg ccggtgtctg ccttctccgc agcggtcagc
      301 gcgtactacc tgccttatgc aggctacagc ctgatgtacc tggcattggc ctgggtcgct
      361 tacggcgtcc ttgccagcgg tatcctgcgg cctaaaacca gcaaggtttg aacgaaaccg
      421 acaagaccgg tcgccacgtt gcgcgcatgc aacgtaaaaa agcgctggtc gatgcgcata
      481 tcgaagcagc ccgtgtcagt cgcgggttgc tactgatcca caccggcaac ggcaagggca
      541 agtcgaccgc cgccttcggc ttgctggcaa gagcgctcgg tcatggtaaa cgggccgccg
      601 tcatccagtt catcaaaagc cgttcagata ccggcgagga agcctttttt cgcacccacc
      661 ccagtgtgca atggcacgtc atgggcgacg gtttcacctg ggagacacag gacgccgagt
      721 ctgaccgcaa gtcgggtcgg gccgcctggg ttcaggtttg ccgctatctg caagatccgg
      781 cgatcgatct cctgatccta gacgaattca cctatgcgct gaaatatggc tggctcagtc
      841 tcgaagaggt ggccgaagcc ctccacaagc gccccgccat gcagcacgtc gtcatcacgg
      901 gtcgcgccgc gccgcctgag ctgatcgcgc tggcggatac ggtcagcgaa gcgctgccca
      961 tcaagcacgc cttctcggcc ggggtagaag ccatgcccgg tatcgagttc tagcctgtgc
     1021 gcactgacgc cagcaatact gaagagccga tcatgcctag cgtaagctgt cctgccctgc
     1081 tgctttctgc gcctgcttca gggcagggca agaccatgac ctccgctgct ctcgcccgcg
     1141 cctggcgcga tcgtgggcta cgcgtacagg tgttcaagtg cggaccggat ttcatcgacc
     1201 cgatgatcct cgaagtcgcc agcggccatc cggtctacaa ccttgatctt ggcatgtgtg
     1261 gtgaagcgga tgtgcgcgaa cgcctatacc gtgccgcgca aaccagcgac gtactcctga
     1321 tcgagggcgt catgggcctg ttcgacggtc agccttccag cgcggatatc gctatccgtt
     1381 tcggcatccc ggtggcattg accatcgacg ccagcgccat ggcgcagacc ttcggtgcgc
     1441 tggcctcagg gctgttgggt catgacccgg cgatccggcc tgcaggcgcc atcgccaaca
     1501 atatcggcag cgcggggcac gccgaattcc tgcacagcag ccttcccgcc cacatcccat
     1561 ggctgggcgc actgccaaag gatacacgct atcacctgcc agaacggcat ttgggcttgc
     1621 atcttgcgca tgagatcgct gacctggaag cgcggatcgc gctagcggcg aaaacattga
     1681 ccgcgaacgc caagctgacg ctgcccgagc cggtgccgtt ccacgccgaa accttgccgg
     1741 ccatgccacg tttgctagac ggaaaaacca ttgccgtcgc ccgtgacgag gcgttctgct
     1801 tcctctaccc ggccaacctc gattgcctgc aggcgctcgg cgcgcgcctg acgttcttct
     1861 cgccgctgca cgacagcgcg ttgccggagg ccgaagcggt ctggctgccc ggcggctatc
     1921 ccgaactgca catgtctacg ctctccgcca accaaggcat gcgagattcc ttgcgacagg
     1981 cgctggccgg cgacattccc atgcttgcgg aatgcggcgg catgatggcc ctatctgctt
     2041 cgatcgacga caggcccgca ttcggactgc tgccaggcaa gagccg
//
LOCUS       DATMTW010000367         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_265535, whole genome shotgun sequence.
ACCESSION   DATMTW010000367 DATMTW010000000
VERSION     DATMTW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265535"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1170)
                     /locus_tag="VN084_07190"
     CDS             complement(295..1170)
                     /locus_tag="VN084_07190"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HWT29280.1"
                     /translation="MPLHRRLAALLLCLALATAGACSLLKMSYNRAPALTYWWLDGYL
                     DFDAAQKPVMQRALARLHAWHYVHELPAYLHLLDDLQQLVNETVTPEQVCRIVDRVRG
                     HALALNTQAAGITMEIALSLTARQLVRLAQKFEDRNEDWRENWMEGTLKERQAYRLKQ
                     AIKRARYLYGSVEDAQESLLSKAIAESSFDPEVSYAAVLQREHAALDLLHAIAERRLA
                     GNAAAAAITQFYDTLLTPQDPAYASYIRTLTLESCRLIAEFHNTTTPAQRQHATERLH
                     GYRDDLSSVRAPVTP"
     gene            complement(1177..>2510)
                     /locus_tag="VN084_07195"
     CDS             complement(1177..>2510)
                     /locus_tag="VN084_07195"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWT29281.1"
                     /translation="YCAFIFDTEILDQLTDKADRRVEFLWESVRELKAALQASGGDLL
                     VRHAPASSAIPALASELGVAAVFSNQDYEPAAIERDRRVAKALEKQCIDFLQYKDQVI
                     FEKDEVLTQAGRPFGVFTPYKNAWLKKLDDFHVRAYPVDRYLGHLAKPALDAGLPTLE
                     AMGFRRTNLADMQLPTGMAGGQRLFADFLDRIDRYKDARDFPAIKGPSYLSVHLRFGT
                     VSIRHLARTAWQLGGKGAEGWLNELIWRDFYFNILHHHPRVAAGHSFKPEFERLPFPN
                     DDRLFAAWCDGRTGYPLVDAAMRQINQTGWMHNRLRMVAASFLVKDLLIDWRRGEAYF
                     ADKLIDFDLSANNGGWQWAASTGCDAQPWFRIFNPVTQSERFDPQGKFIRKYVPELAA
                     CDDKEIHAPWLIPPLRQQALRLQIGSDYPAPIVDHASQRQSALSLYKQAGA"
BASE COUNT          484 a          702 c          782 g          542 t
ORIGIN      
        1 ttggcctgag aggcacaccc cgtaagcgat aacgcgagaa ccatgctggc gatcagagtt
       61 gttgtattta tatttatacg catgctttat tccgaaattg tttcgttgtg ctgaggtttc
      121 cagcatccat cgcgaacttt aaccgagcga taccccattg tccactgttt taggcccatg
      181 cgcgacaggt gttgcaaaaa tgtaacaccc tgataaacca taatattatc aatgggatat
      241 aggggtgttt tgacgatatt ttcgacctgg aagcgggctt tcgacagact gtcatcaagg
      301 tgtcacaggc gcgcgaactg atgacagatc gtccctgtag ccatgcagcc gctcggtcgc
      361 atgctgacgc tgtgccggcg tggtcgtatt gtggaattcg gcgatcaggc gacatgactc
      421 caaggtcagg gtccgtatgt agctggcgta ggcggggtct tgtggcgtga gcagcgtgtc
      481 atagaactgc gtgatggccg ctgctgcggc attcccggcc aatcgacgct cggcgatcgc
      541 atgcaatagg tccagtgccg catgttcccg ctgcaggacg gcggcatacg aaacctcggg
      601 gtcgaagctt gattctgcga tcgccttgct gagcagcgat tcctgcgcgt cttcgacact
      661 gccgtagaga tacctggccc gcttgatcgc ctgtttcaat cggtaagcct gtcgttcttt
      721 caatgtgcct tccatccagt tttcccgcca gtcctcgtta cggtcctcga atttttgcgc
      781 aaggcgcacg agctggcgcg cggtcagact cagcgcgatc tccatggtga tgccggcggc
      841 ctgggtgttg agggctaggg cgtgtccgcg cacccggtcg acgatcctgc acacttgttc
      901 gggcgtgacg gtctcgttga ccaactgttg cagatcgtcg agcaagtgca ggtaagccgg
      961 cagttcgtgt acgtaatgcc aggcatgcaa ccttgccagt gctcgctgca tgaccggttt
     1021 ctgtgcggca tcgaaatcca gatagccgtc cagccaccaa taggttagcg ccggcgcccg
     1081 gttgtagctc atcttgagca ggctgcaagc gccagcagtg gccagcgcaa ggcagagtaa
     1141 gagtgctgcc agacggcgat gcagcggcat gcgggcttat gccccggcct gcttgtagag
     1201 tgacagcgcc gattgccgct ggctggcgtg atcgacgatc ggcgcaggat agtcgctgcc
     1261 gatctgcagg cgcaaggcct gctgtcgcaa gggcgggatg agccagggcg catggatctc
     1321 cttgtcgtca catgcagcca gttcggggac atatttgcgg atgaatttgc cttgcgggtc
     1381 gaatctctcc gattgcgtga ctggattgaa gatgcggaac cagggctgcg cgtcacagcc
     1441 ggtagaggcc gcccattgcc agccgccatt gttggcgctg agatcgaagt cgatgagctt
     1501 gtcggcgaaa tacgcttccc cccggcgcca gtcgatgagc aagtccttca ccaggaaact
     1561 ggcggcgacc atgcgcaggc ggttgtgcat ccagccggtc tggttgatct ggcgcatggc
     1621 ggcatccacc aggggatagc cggtgcgccc atcgcaccag gcggcgaaca gcctgtcgtc
     1681 attggggaag ggcagtcgct cgaattccgg cttgaaacta tgcccggcgg cgacgcgcgg
     1741 atgatggtgc aggatattga aatagaaatc gcgccagata agctcgttga gccagccttc
     1801 tgcgcccttg ccgccgagtt gccaggccgt gcgcgccagg tgacggatag agacggtgcc
     1861 gaagcgcagg tgcacggaaa ggtaggaggg gcctttgatt gccgggaagt cgcgcgcatc
     1921 cttgtaacgg tcgatacgat caaggaaatc cgcgaacagg cgctgtccgc cggccatgcc
     1981 ggtcggtaac tgcatatccg cgagattggt gcgcctgaat cccatggctt ccagtgtcgg
     2041 cagcccggca tcgagcgccg gttttgctag atgaccaagg tagcgatcga ccgggtaagc
     2101 ccggacatgg aaatcatcaa gttttttcag ccaggcgttc ttgtacggcg tgaagacacc
     2161 gaacggcctg cctgcctgcg tgaggacttc atccttctcg aagatgacct gatccttgta
     2221 ttgcagaaaa tcgatacact gcttttccaa ggccttggcc acgcggcggt cgcgttcaat
     2281 ggcggccggt tcgtagtcct ggttgctgaa caccgcagcc acgcccaact cgcttgccaa
     2341 tgcgggtatc gcgctactgg ccggcgcatg ccgtacgagt aggtcgccgc cggacgcttg
     2401 caaggctgcc ttgagttcgc gcacgctctc ccagaggaac tcgacgcgac ggtcggcctt
     2461 gtccgttagc tggtccagga tctcggtgtc gaagataaag gcgcaataga
//
LOCUS       DATMTW010000368         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_399970, whole genome shotgun sequence.
ACCESSION   DATMTW010000368 DATMTW010000000
VERSION     DATMTW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399970"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="lolA"
                     /locus_tag="VN084_07200"
     CDS             complement(<1..593)
                     /gene="lolA"
                     /locus_tag="VN084_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HWT29282.1"
                     /translation="MNLGIVLRSTLVALSLSVPVLAHAGGVDSLRTFFQTTQSLRADF
                     QQTVVDNQGRKVQEVTGTMRLQRPGKFRWDYNKPYVQVIVGDGARVWLYDPELSQVTV
                     RSLDKALGNTPAALLAGSKEMEKSFTLKDEGRQDDLDWVQATPKTPESGFEKVFLGFR
                     NDRLQEMELHDSFGHMTVIEFSKLETNPKLSPEAFRFV"
     gene            complement(610..2910)
                     /locus_tag="VN084_07205"
     CDS             complement(610..2910)
                     /locus_tag="VN084_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWT29283.1"
                     /translation="MFFRKKTVPLNSRREPVEPVKTHGIVREAWWMILLLVGLYLTVI
                     LFSYHRDDPSWSHSASDSTLIHNAGGAVGAWLSDILLYLFGFSAWWCVVLAFYAIWLV
                     YLRLEVVAAHHKPVLLFNLVGFGILLVSSCALEAGHVIPLPADLPLGAGGMLGDVVDG
                     ILRSMLGFDGSTLVLLMLMAIGFSLFTGWSWIMMTEKLGAGIEISYAFLRDKWQDRQD
                     RKAGRIAEQLRTEFVDNERKRTEDRMPVQIETPAVEIVKSERVQREKQAPLFETLPDS
                     PLPPLHLLDEPHGEVELQSAETLEFTSRLIERKLMDFGIEVKVLTALPGPVITRYELD
                     PAPGVKGSQVANLAKDLARALSVISIRVVETIPGKSCMGLEIPNPKRQVVFLSEILGS
                     QVYADVHSPLAIALGKDIAGKPAVADLAKMPHVLVAGTTGSGKSVAINAMILSLLYKA
                     EPNKVRLILIDPKMLELSVYEGIPHLLAPVITDMRQAGNALNWSVAEMERRYKLMSVL
                     GVRNLAGYNQKIRDAKKEGEPIPHPFSLTPESPEPLDELPMIVVVIDELADLMMVVGK
                     KVEEPIARLAQKARACGIHLVVATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTIL
                     DQMGAEALLGQGDMLYQPPGTSYPQRIHGAFVSDQEVHRVVDNLKSRGEPNYVEGILT
                     GASEGEGEGGELSGEGGGESDPLYDEAVAIVLKSRRASISSVQRHLRIGYNRAARLIE
                     EMERAGLVSAMQSNGNREVLAPKGNE"
     gene            3193..3657
                     /locus_tag="VN084_07210"
     CDS             3193..3657
                     /locus_tag="VN084_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HWT29284.1"
                     /translation="MDIGINEKDRKKTAEGLSHLLADTYTLYLKTHYFHWNVTGPMFN
                     TLHLMFEQQYTELALAVDAIAERIRSLDFYAPGTYRDFIKLASIKESESVPKANDMIR
                     ELVAGHEAVVRTARGVFPAAEKAGDEATADLLTQRLQLHEKTAWMLRSLLEK"
BASE COUNT          763 a         1121 c         1041 g          742 t
ORIGIN      
        1 acgaaacgga acgcctctgg cgaaagtttg ggattggttt ccagtttcga gaattcgatg
       61 acggtcatgt gtccgaaact gtcatgcagc tccatttcct gcagccgatc gttgcgaaag
      121 cccagaaaga ccttttcaaa accgctttca ggcgtcttcg gtgtggcctg tacccaatcg
      181 agatcgtcct ggcgaccctc atccttgagc gtaaaactct tttccatctc cttcgacccc
      241 gccagcagtg cggccggggt gttgcccagc gccttgtcga gcgaacgcac ggtgacctgg
      301 ctcaactccg ggtcatatag ccagacgcgt gcaccatcgc cgacgatgac ctgcacataa
      361 ggcttgttgt aatcccagcg gaacttgccc gggcgttgca atcgcatggt gcccgtgacc
      421 tcctgcactt tgcgcccctg gttatcgacc acggtctgct ggaaatcggc acgcagcgat
      481 tgcgtggtct ggaagaaggt acgcagacta tccacgccgc ctgcatgggc aagcacaggg
      541 acgctgaggc tcagggcgac cagtgtcgat cgcaggacga ttccgagatt catgatgttt
      601 tcattcacgc tattcattcc ctttgggggc caacacttcg cggttgccgt tgctctgcat
      661 cgccgacacg aggccggcac gttccatttc ttcgatcagg cgggcagcac ggttataccc
      721 gatacgtagg tggcgttgta ccgatgagat cgatgcgcga cgcgacttga gcacgatagc
      781 gaccgcttcg tcgtacaagg ggtcggattc cccaccgcct tcgcccgata gctccccacc
      841 ttcaccctca ccctcgctgg cgccggtgag gatgccctcg acatagttcg gctcaccacg
      901 tgacttgagg ttatccacta cacggtggac ttcctggtcg gagacgaacg cgccatggat
      961 acgctgcgga tagctggtcc ctggcggctg gtagagcatg tcgccctggc cgagcagggc
     1021 ttcggcgccc atctggtcca ggatcgtgcg cgaatcgatc ttgctcgaca cctggaaagc
     1081 gatacgcgta gggatattgg ccttgatcag gcccgtgatg acatcgaccg atggccgctg
     1141 tgtcgccacc accaggtgga taccgcaagc ccgggctttt tgcgcgaggc gcgcgatcgg
     1201 ctcttcgacc ttcttgccca ccaccatcat aagatcggca agttcgtcga tcacgaccac
     1261 gatcatcggc aactcgtcca atggctccgg gctttccggc gtgagactga acgggtgcgg
     1321 gatgggctca ccctcttttt tcgcgtcacg gatcttctgg ttgtagcccg cgagattacg
     1381 cacgccaagc actgacatca gcttgtagcg ccgttccatc tcggcgacgc tccagttgag
     1441 cgcgttaccg gcctggcgca tatcggtgat gaccggtgcc agcaaatgcg ggatgccttc
     1501 gtagaccgag agttccagca tcttgggatc gatcaggatc agccgcacct tgttcggttc
     1561 agccttgtat aacaggctga ggatcatggc gttgatggcc accgacttac cggaaccggt
     1621 ggtccctgcg accagcacat gtggcatctt ggcgaggtcc gccaccgcgg gcttgccggc
     1681 gatatccttg cccagtgcga tggccagtgg cgaatgtacg tcggcataga cctgggaacc
     1741 gaggatctcc gaaaggaaca ccacctggcg tttgggattg gggatctcga gacccatgca
     1801 gctcttgccc gggatggtct cgaccacacg gatgctgatg accgatagcg cgcgcgccag
     1861 gtctttcgcc agattcgcca cctggctacc cttgacgcca ggcgccgggt ccagctcata
     1921 acgcgtgatg acagggcctg gcaaggccgt caacaccttg acctcgatac cgaagtccat
     1981 gagcttgcgc tcgatcaggc gtgaggtgaa ttcgagggtc tcggcagatt gcagctcgac
     2041 ctcgccatgc ggctcatcca gcagatgcag cggcggcagt ggcgagtcag gcaaggtctc
     2101 gaacaagggc gcctgcttct cacgctggac gcgttcgctt ttgacgatct cgaccgcagg
     2161 cgtctcgatc tggaccggca tgcggtcttc agtgcgcttg cgttcgttat cgacgaattc
     2221 ggtacgcaat tgctcggcga tgcgccctgc cttgcgatcc tgccgatcct gccatttgtc
     2281 gcgcaggaat gcgtaactga tctcgatgcc tgcgcccagc ttctcggtca tcatgatcca
     2341 cgaccaaccc gtgaacaggc tgaatccgat cgccatcagc atcagcagga ccagggtcga
     2401 gccatcgaaa cccagcatcg atcgcagaat accgtcgacc acatcgccga gcatgccgcc
     2461 cgcgcccaat ggcaggtcag ctggcaaagg gatgacgtgc ccggcttcca gcgcgcagga
     2521 cgacaccagc aggatgccaa agcccacaag attgaacagc agcacgggtt tgtgatgcgc
     2581 ggcgaccact tccaggcgaa ggtagaccag ccagatggca tagaacgcca gcacaacgca
     2641 ccaccatgca gaaaacccga acagatagag caggatatcc gagagccagg caccgactgc
     2701 gccacccgca ttgtggatca gggtgctgtc gctggcgctg tgcgaccatg aagggtcgtc
     2761 gcgatggtag ctgaacagga tcaccgtgag gtagagcccg accagtagca ggatcatcca
     2821 ccaggcctca cggacgatgc cgtgtgtctt tacgggctcc acaggttcgc ggcggctatt
     2881 gagcgggacg gttttttttc ggaaaaacaa gatgttttac atgcccagaa caaggttgga
     2941 atcaggcccg attatagcag tatcgattgc ccgcggtgtg tcgcctcgcc agatgtcgcc
     3001 atgccatgcg cgacccgcgt tcggctgcgc cggagctatc atgcacgcct caagcatgcg
     3061 cgcctcaagt caccccataa accgcgccta tcgcgccgat ttgtacaatc aatttgcgcc
     3121 atcatcgcca tgaacctaag ataacggcaa ttgtcatttt tagtatcgaa tacccaccat
     3181 caggagaagc aaatggatat cggcatcaac gaaaaagatc gcaagaagac cgctgaaggc
     3241 ctgtcgcact tgctggcaga cacctatacc ctctacctga agacgcacta tttccactgg
     3301 aatgtcacgg ggccgatgtt caacaccctg catctgatgt tcgagcagca atatactgaa
     3361 ctcgcactgg cagtggatgc catcgccgag cgcatccgct ctctcgactt ctacgcgccg
     3421 ggtacttatc gtgatttcat caagctcgcc tcgatcaaag agagtgaatc cgtccccaag
     3481 gccaatgaca tgatccgcga actcgtggcc ggccacgaag cggtggtacg cacggcgcgt
     3541 ggcgtcttcc ccgctgccga gaaagctggt gacgaagcga ccgccgacct cctgacccag
     3601 cgcctgcaac tgcatgaaaa aacggcctgg atgttgagaa gcttgctgga aaaatgaggc
     3661 cgctttc
//
LOCUS       DATMTW010000369         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_65957, whole genome shotgun sequence.
ACCESSION   DATMTW010000369 DATMTW010000000
VERSION     DATMTW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65957"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VN084_07215"
     CDS             complement(<1..750)
                     /locus_tag="VN084_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWT29285.1"
                     /translation="MYYAHFGLKQPPFKITPNTDFFFSGGNRGAVLDALIYAITSGEG
                     IIKVVGEVGSGKTMLCRMLQTLLPEKVESIYLANPSVAPEDVLHAIAFELQLKLPKNA
                     DRLQVMQVLQKYLLDRHEQGKQVVIFVEEAQGMPIATLEEIRLLSNLETKHDKLLQIV
                     LFGQPELGINLNQTQIRQLRERITHGFNLGPLEEKDVGEYLIFRLRTAGYFGPHLFNK
                     ASIRRLYKASQGLVRRVNILADKALLATFAEN"
     gene            complement(763..2196)
                     /locus_tag="VN084_07220"
     CDS             complement(763..2196)
                     /locus_tag="VN084_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWT29286.1"
                     /translation="MAAQRPVLIIIDDDPLITDTLHYVLSKYFEVCVAESRAQAKSLL
                     RQLETPPSLALVDLGLPPVPHKPDEGFKVIGELLAHSPTMKILVLSGQNEEVNVKHAL
                     ALGAVDFIPKPCDVERLNELLQNALMMQDAELGAISREEDDGGLLGSSLPMQTLRTQI
                     SQFADAPFPVLIEGESGSGKELVANCLHRLSKRSKQPYLSVNCAAISPMLAESILFGH
                     GKGAFTGAANSHTGYFEDAGEGTLFLDEIGELPLELQAKLLRVLENGEYQRIGETQTR
                     KSRARIVAATNRDLRDEVRQGYFRIDLYHRLSVFSVRVPPLRELEDDRYLLLEHFSEF
                     YARETNQKSFELEPKAKQRWLDYHFPGNVRELRNIIIRLIAKYAGQSVSDTQLAAELD
                     LAQQLAAPNEMPLVNDENGLANYAHRHLQTQANVNLDVVLKAWEKAYVDAALKITHGN
                     LSQAAKLLGINRTTLYSRIQLQEDFKD"
BASE COUNT          440 a          637 c          690 g          507 t
ORIGIN      
        1 attttcggca aaggtagcga gcaaggcctt gtcagccagg atgttcacac gccggaccaa
       61 gccctgcgag gctttgtaca aacgccggat cgaggcctta ttgaatagat gtggtccgaa
      121 atagcccgcc gtccgcaatc ggaagatcag gtattcgccc acatccttct cttccagcgg
      181 gcccagattg aagccatgcg tgatgcgctc cctgagctgg cggatctgcg tctggttgag
      241 gttgataccc agctcgggct ggccgaagag aacgatctgc aacagtttgt cgtgcttggt
      301 ttcaaggttg gacaagagcc ggatctcttc cagcgtggcg atcggcatac cctgcgcctc
      361 ctcgacgaag atcaccactt gcttgccttg ctcatggcga tcgagcaggt atttctgcag
      421 cacttgcatg acctgcaggc gatcggcgtt ctttggcagc ttgagctgca actcgaaggc
      481 gatggcatgc agcacgtcct cgggcgcaac gctggggttg gcgaggtaga tgctttcgac
      541 cttttccggc aacaaggtct gcaacatgcg gcagagcata gtcttgccgc tacctacttc
      601 gcccacgacc ttgatgatgc cttcgccact ggtgatggcg tagatcagcg cgtcgagtac
      661 cgccccgcga ttgccgcctg aaaaaaagaa atcggtattg ggtgtgatct tgaacggcgg
      721 ttgcttgagg ccgaaatggg cgtagtacat aagtgtgcgg ccctagtcct tgaaatcctc
      781 ttgtagctgg atgcggctat acagcgtagt gcgattgatg ccgagcagct tggcggcctg
      841 actcaggttg ccgtgggtga tcttgagcgc ggcatcgacg taggcctttt cccaggcctt
      901 gagcacgaca tcgagattga cgttggcctg ggtctgcaga tgtcggtggg catagttggc
      961 cagcccgttc tcgtcgttga ccagcggcat ctcgttgggt gctgccagtt gctgggcaag
     1021 gtccagctcg gccgcgagct gggtatcgct gaccgattgt cccgcatatt ttgcgatcag
     1081 ccggatgatg atattgcgca gctcgcgcac attgccgggg aagtgataat ccagccagcg
     1141 ctgtttcgct ttcggttcaa gctcgaaact cttctggttg gtctcgcgcg catagaactc
     1201 gctgaaatgc tccagcagca ggtaacgatc gtcttccagc tcccgcaagg gcggtacacg
     1261 gacgctgaac acgctcaagc gatgatagag gtcgatgcgg aaatagcctt gcctgacctc
     1321 gtcgcgaagg tcgcggttgg tcgccgccac gatacgcgca cggctcttgc gggtctgggt
     1381 ctcaccgatg cgctggtatt cgccgttttc cagcacacgc agcaacttgg cctgcagttc
     1441 gagcggcaac tcgccgatct cgtccagaaa gagcgtgcct tcgcctgcat cctcgaagta
     1501 gccggtatgt gaattcgccg cgccggtgaa ggcgcccttg ccgtggccga acaggatgga
     1561 ttccgccagc atgggggaga tcgcggcaca gttgaccgag agatacggct gtttggatcg
     1621 cttgctcaaa cggtgcaggc aattcgccac cagctccttg ccgcttccgg actcgccctc
     1681 gatcagcaca ggaaaaggcg catcagcgaa ctggctgatc tgggtgcgca aggtctgcat
     1741 gggcaggctg ctgccgagca ggccgccgtc gtcttcctcg cgactgatgg cgccaagctc
     1801 ggcgtcctgc atcatcagcg cgttctgcaa cagctcgttc aggcgctcga catcgcaagg
     1861 cttgggaatg aaatcgacag cgcccaatgc cagggcgtgc ttgacgttga cctcttcgtt
     1921 ctggcctgaa agcaccagga tcttcatggt cggcgaatgg gccagcagtt caccgatgac
     1981 cttgaagccc tcatccggct tgtgcggcac cggcggcaat ccgagatcca ccaatgccaa
     2041 tgatggtggc gtttcgagct gacgcagcag gctcttggct tgggcacggg attccgccac
     2101 acagacttcg aaatacttgc tgagcacgta atgcagagta tcggtgatca ggggatcatc
     2161 gtcgatgatg atgaggacgg gacgttgtgc agccatcaaa cagactttct gttgtgtgcg
     2221 aggcgattga tcttaggttt caacgcgtac tatagccgca ttgcggtgtg ctat
//
LOCUS       DATMTW010000370         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_133571, whole genome shotgun sequence.
ACCESSION   DATMTW010000370 DATMTW010000000
VERSION     DATMTW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133571"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..>3463)
                     /locus_tag="VN084_07225"
     CDS             complement(12..>3463)
                     /locus_tag="VN084_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB pore-forming domain-containing
                     protein"
                     /protein_id="HWT29287.1"
                     /translation="LIGLVRQALAEEKLIARTVAPVVKAALGSSVSEATGGVLQLANV
                     GFDIYQLSTASSEIERARFGTQLAFDSAGLVLAAGALGLGATTAGAFLGGAAVILGGL
                     AVGVAAVTQGFAAIAEEAKQVGLFFDELSKAHLQAYRFDTHSDAWLPRPSLIVGTLDL
                     VRGELVLDSPRLYPLRDHFGVPTFDDDYDEAIDIRRELRLVDRVRFSPSAGQAIVLPC
                     VPRTCYRYEYKALPFAGLRHDVGFDTARRLEKKKADGGWLFLFSFYSFPSDYILYRLL
                     PDYRPTVIDVVLDEAERSLVVPVLPAQWQQKISYRIKGAGKRCTVGLNPGVSLTLESL
                     NRQKSSWVLDAPWANEGDIRVERYAKLFVGTVQVSFAGTGLHDVLLRIAGQQVFQVGL
                     GERTLTLVEQEVPAGLDPQALQPHLRSLAQGHRLVMPYTPLHRYLVPFEKLDEPRHVT
                     AWYDAKEDRFLYIRNEIPGTDDVLLGAVAGDSAYFYDPKGLIVWQADATTGLLSHRYW
                     LRSARDAATTISRVEADAQGVVHVEQRITRADQTRDVLVYVIHDGQLLLSSVTREAGP
                     ALESVFSASETLADWSQVLGNGYPFTPYVDNEAAYITVSWQPAAFVSVCWKIDAQWRD
                     MAWVRRSDQLIIRPAPKHNHLRGWPDSIKNMTDLTLLTPKDGSDVFIVYDRPAKALCR
                     RRRILVADKWQWSSKWKQHEKLENIIAIDDGYLVLTSDGLFYTLTDQGELSLGGMNAP
                     WFKNRTHWWSALEPLARRHATERLALVGLTNFSGDAKLCAWYIGNRLLLAEPGHGKEM
                     RLLSVTPDGEAAWLFDVSTGGVYRQAFIDPQKLESVFEQGTRVLQADALPTAQREWAS
                     WTFVEVTVEGAGLRGVTSGGVVVSLRNRELALITGVTREWVIAQDGREQKALEQLADQ
                     PSCSALLTVEDPDDLKWFVTERKRLVRVSRADIPGTFDVLGTQRQDNVLLHEQSKSRL
                     LSYPAIEEAGSVGYAQREGEVLVVGAEKMKIDDLLPDDVTTLVLRMGQGSVSCRLTKA
                     AWLRLESVVLDCRPSLDGTSAEPGKLIWELDDPDRLLLSIVDEHLVIIDPDTGHSVIV
                     REMYAGDINFRGEVLLSFEEHRRYAVSKLIKRLDAQPNPRNSITLKELLGVSQRMESN
                     AVT"
BASE COUNT          689 a         1165 c          971 g          638 t
ORIGIN      
        1 ttatagcctg atcaggtcac cgcattactt tccatcctct gcgaaacgcc cagcaattcc
       61 ttcagcgtga tgctgttgcg gggattcggc tgggcgtcca agcgttttat cagctttgaa
      121 acggcatagc gtcgatgttc ctcgaaactg agcaagacct caccgcgaaa gttgatatcg
      181 ccggcataca tctcacgaac aatcacactg tgcccagtgt caggatcaat gatcaccaag
      241 tgctcatcga caatgctcag cagcagccgg tccgggtcat ccagctccca gatcaacttg
      301 ccgggctcgg cagacgtgcc atccagagaa ggccggcaat cgagaacgac cgattcgagc
      361 ctcagccagg ccgccttcgt cagccgacaa ctgacactcc cctgccccat gcgcaggacc
      421 agggtcgtaa catcgtccgg cagcagatca tcgatcttca tcttctccgc gccgacgacc
      481 aggacctcac cctcacgctg ggcgtagccg acggatcccg cctcttctat ggccgggtag
      541 ctcagcagcc tgcttttgct ctgttcatgc agcagcacgt tgtcctgtcg ctgggtcccc
      601 agtacatcga aggttcccgg gatatcggcc ctggacaccc gaaccaggcg cttgcgctca
      661 gtgacgaacc atttcaggtc gtcgggatcc tccacggtca gcaacgcgct acaagacggc
      721 tggtccgcca attgctcgag cgccttctgc tcccgaccat cctgggcaat gacccattca
      781 cgcgtcacgc ccgtgatcaa cgccagctcg cgattgcgca gcgaaaccac gacacctccg
      841 gatgtcacgc cccgcagccc ggcgccttcg accgtcacct cgacaaatgt ccaggacgcc
      901 cattcacgct gcgccgtggg taacgcatca gcctgcagca ctcgcgtgcc ctgctcgaag
      961 accgactcaa gcttctgcgg atcgatgaag gcctggcgat acactccgcc agtcgagaca
     1021 tcgaacagcc aggccgcctc gccatctgga gtgacgctca gcaggcgcat ttccttgcca
     1081 tgccccggtt cagccagcag cagcctgttg ccgatgtacc aggcacacaa cttcgcgtca
     1141 ccgctgaaat tggtcagacc cacaagggcc agacgctcgg tggcgtgtcg tcgggccagc
     1201 ggctccaatg cagaccacca atgtgtccga ttcttgaacc atggcgcgtt catgccgccc
     1261 aggctcagtt cgccctggtc cgtgagggtg tagaacagcc cgtcggaagt cagtaccagg
     1321 tagccatcgt cgatggcgat gatattttcc agtttctcgt gctgcttcca cttactcgac
     1381 cattgccact tgtccgccac caggatgcgt cgccgacggc acagcgcctt tgcgggtctg
     1441 tcgtatacga tgaacacatc gctgccatct ttcggcgtga gcaaggtcag gtccgtcatg
     1501 ttcttgatcg agtccggcca gccgcgcaga tgattgtgtt tcggcgcggg gcggatgatc
     1561 agttggtcgc tgcgacgaac ccaggccatg tcccgccact gcgcatcgat tttccagcag
     1621 accgacacga aggcggcagg ctgccaactg acggtgatgt aagccgcctc gttgtcgaca
     1681 tacggtgtga aggggtaacc gttgcccagg acctggctcc agtcggccag tgtttcgctg
     1741 gcgctgaaca ccgattccag cgccggcccc gcttcccggg tcaccgaact caacagcaat
     1801 tgcccgtcat ggatcacata gaccagcaca tcgcgggtct ggtcggcgcg ggtgatgcgc
     1861 tgctcgacat ggaccacgcc ctgggcgtcc gcctcgacgc ggctgatggt ggtcgccgca
     1921 tccctggcac tgcgtagcca ataacgatga ctcaacagcc cggtggtcgc gtcggcctgc
     1981 cagacgatca aaccctttgg gtcgtagaaa taggcgctgt cgcccgccac cgcccccaac
     2041 agcacgtcat cggtaccggg gatctcgtta cggatgtaga ggaaacggtc ctccttcgcg
     2101 tcataccagg ccgtgacgtg acggggctcg tcgagcttct cgaaaggaac caggtagcga
     2161 tggagcggcg tgtagggcat caccaggcga tgaccttgcg ccaggctcct gaggtgcggc
     2221 tgcagcgcct gtggatccaa gcccgcaggc acctcttgct cgacgagcgt gagtgtgcgc
     2281 tcacccaacc cgacctggaa cacttgttga ccagcgatcc gcagcaacac gtcatgaaga
     2341 cccgttcccg cgaagctcac ctggacggta cccacgaaca gcttcgcgta gcgctcgacc
     2401 cggatgtcgc cctcattggc ccagggagcg tccagtaccc agctcgactt ctgacggttc
     2461 agcgactcga gggtgaggct cacgccggga ttgagcccca ctgtgcagcg tttgcccgcg
     2521 cctttgatcc gatagctgat tttttgctgc cactgcgctg gaagcacagg caccaccagc
     2581 gaccgctcgg cctcatcgag caccacgtcg atgacggttg gccgatagtc cgggagcagg
     2641 cgatacagga tgtagtcgct cggaaacgaa tagaacgaaa acaggaacag ccatccgcca
     2701 tccgcctttt ttttctccag ccgccgtgcc gtgtcgaagc cgacgtcatg acgcaatcct
     2761 gcgaatggca acgccttgta ctcatagcga taacaagttc tcggcacgca aggcagtacg
     2821 atggcctggc cagcggacgg gctgaacctg acccgatcga ccagccgcag ctcccgacgg
     2881 atatcgatcg cctcgtcata atcgtcatcg aacgtcggca cgccaaaatg atcccgcaac
     2941 ggatacagcc tcgggctgtc cagaaccagc tcgccacgca ccagatccaa cgtgccgacg
     3001 atcagcgacg ggcgcggcaa ccaggcatcg ctatgggtat cgaaacgata ggcctggaga
     3061 tgggccttgc tcagttcatc gaagaacagc ccgacctgtt tggcttcttc ggcgatggcg
     3121 gcgaacccct gggtcactgc cgctacgcct accgccaggc cgcccaggat cacggcagcc
     3181 ccgccaagaa acgctccagc ggtggtggcg ccgaggccga gcgctcctgc cgccagcacc
     3241 agcccggccg aatcgaacgc cagctgcgtg ccgaaccggg cgcgctcgat ctcgctggac
     3301 gcggtcgaca actgatagat atcgaacccg acattggcca gttgcagcac gcctccggtc
     3361 gcctcgctca cgctggatcc cagcgccgcc ttgaccacag gcgcaacggt gcgggcaatc
     3421 agtttttctt ccgccagcgc ttgccggacc aaaccgatca agc
//
LOCUS       DATMTW010000371        10139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_400344, whole genome shotgun sequence.
ACCESSION   DATMTW010000371 DATMTW010000000
VERSION     DATMTW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400344"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /gene="glyS"
                     /locus_tag="VN084_07230"
     CDS             <1..1878
                     /gene="glyS"
                     /locus_tag="VN084_07230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWT29288.1"
                     /translation="LDAEGKATPALLKKLAALGADEGAVATLKRENDGKADILFLDAM
                     IQGATLQEGLQKALDEALAKLPIPKVMTYQLADGWSNVNFVRPAHGLVALHGSDIVAI
                     ETLGLKAGKTTQGHRFEAKHPIVTLKDADSYAAQLKDEGAVIASFAERRAEIVRQLSA
                     AAAKENLKPIDDDALLDEVTALVERPNVLLGQFEQEFLEVPQECLILTMKANQKYFPL
                     LDSKGKLTNKFLIVSNIAPSDASAVIGGNERVVRPRLADAKFFFDQDRKKSLASRVPG
                     LAKVVYHNKLGTQGERTERVRKIAGWVAQKLGADPKLADRAAELAKADLLTDMVGEFP
                     ELQGIMGGYYARHDGENELVAAAIADQYVVKRDDEESASNLVSESLLIADRVETLVGI
                     WAIGLKPTGEKDPFALRRHALGVIRSFELCHAAARIGKQAFALKLSELLAYAASTFDS
                     VKIEPEILAEIHSFIYERYRNQLAADYKPQTLDAVISLNPELHEVIIRSEAVKAFQQL
                     SESESLAAANKRVGNILKKADETVALTVVDHLLKEPAEAALAQALSKVKPQADQAFEA
                     GDYAGSLKSLAVLKAPVDDFFDNVMVNAEDPALRANRLALLSQLHAAMNRVADLSKLA
                     A"
     gene            1915..2532
                     /gene="gmhB"
                     /locus_tag="VN084_07235"
     CDS             1915..2532
                     /gene="gmhB"
                     /locus_tag="VN084_07235"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HWT29289.1"
                     /translation="MKLVILDRDGVINYDSAQFIKSPDEWKPIPGSLEAIALLNQSGY
                     RVALATNQSGIGRGLFDMATLNAIHDKMHRALAQLGGRIDALFYCPHAADAKCDCRKP
                     KPGMIEEIGRRFAVDLAGVPSVGDSLRDLEASAALGAQPILVKTGKGECTINNGGLPA
                     GTWICDDLAQAVQRIIAEDKPVAEKHARDKSPAKAVKDKSSKESR"
     gene            2532..3242
                     /locus_tag="VN084_07240"
     CDS             2532..3242
                     /locus_tag="VN084_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWT29290.1"
                     /translation="MIVLRSLLFTLGQWLFTPLFTLFAILTFPFKPVTRYRFISHWAR
                     IMLWWLRVTCGVRYRVIGREHIPPHASVILSKHQSAWETLAFQEIFPPQVWVLKRELL
                     WVPFFGWGLAMTSPIAIDRSAGREALKQMVAQGRDRLQQGFWVVIFPEGTRIAPGKKG
                     KYHIGGAWLATHTASTVVPVAHNAGEFWPKNAFIKRPGTITVSIGPPIDSSGLKADEL
                     NQRVERWIESQMPLLEHT"
     gene            3239..3961
                     /locus_tag="VN084_07245"
     CDS             3239..3961
                     /locus_tag="VN084_07245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWT29291.1"
                     /translation="MSEHLLTLADGAQIPYLLERRPRRTVGMRIGAQGLVVHAPTRLS
                     LSHLETLLLGKAQWILNKLQLHATHAVAPFQWRDGDNLLWLGNEMLLSVRHDSRSRAI
                     MHEPGQLHVALPTPHDAATVSRKVLLWYRKQAQADFSRRLQILAARLGVDTPPLYLSN
                     ARSRWGSCNSRGEIRINWRLIQAPPHIINYVICHELAHLKEMNHSARFWAVVAQLHPD
                     YKRAEQELKALSARLHQLDIHS"
     gene            complement(3944..5812)
                     /locus_tag="VN084_07250"
     CDS             complement(3944..5812)
                     /locus_tag="VN084_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HWT29292.1"
                     /translation="MKAQHGSSTAALTLGAIGVVYGDIGTSPLYTIREIFSASTGIPL
                     TPANLVGAVSTVFWALMLVVTLKYVILVLRANNHGEGGVMALLALAVTKAKQQHRPWL
                     LLLGVMGGALFYGDSVLTPAISVLSAIEGLQVVSPGLASFVMPLSAAILFGLFAVQRF
                     GTQAVGKLFGPVIIVWFLVLGAIGLWHIVHAPAILAALNPMHALTFLMARGSGVFLAV
                     GAVVLAITGAEALYADMGHFGKPAIRIAWNGLVLPGLALNYMGQGALLLDHPAAVSNP
                     FYLSFPPALLVPAIILATMATIIASQAVISGAYSITRQAIQLGFLPRMRIVHTSATES
                     GQIYLPAVNWLLLVAVLTVTVSFGNSSALATAYGIAVTGTMLITTILTFFVLRYGWRY
                     PLWLAAGATGCFVALDSLLLVSCSVKFLQGGWFPVVVAAGMLLLMWTWKQGRDALLKS
                     IRQDDPLLQNFVSSMADSTLPRIERTAVFLVSDPHSAPQALMHNLKHNQVLHRTNLVL
                     TVEYADTPTVASNARSHHQALGHGFWQMTLRYGFMENPDIPKALAQCPLPGVVIDPFT
                     TSYFVSREVVVPSAGGRMAKWRDSLFAAMSRNAGSVVEYFNIPINSVIELGSRVYI"
     gene            complement(5890..6579)
                     /locus_tag="VN084_07255"
     CDS             complement(5890..6579)
                     /locus_tag="VN084_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWT29293.1"
                     /translation="MTDNATVLVVEDDREISRFLAASLQAAGFKPVSARTLAEALHQM
                     VSLTPNMVILDLGLPDGDGADFIRSLRKHSDIPILVLSARQSERQKVDSLNLGADDYL
                     QKPFGVEELLARVRVALRHVSLMTLRDQVYEVGGLRIDRGSGTVTLEGHPVHLSKTEH
                     KLLMSLAQRPGRIYPHRELLATVWGPEYVDDTHYLRIHMGRLRAKLEADPGQPRYLLT
                     ELGIGYRLANQ"
     gene            complement(6572..8029)
                     /locus_tag="VN084_07260"
     CDS             complement(6572..8029)
                     /locus_tag="VN084_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWT29294.1"
                     /translation="MRPSFALLAIAVLTIVVSAVVYLNQDTLGMAGTVTLYLLVVLAG
                     AYYLQVTLALSLALCATLLINFLVVEPRYTFRIASVESWVALGGFLTVSLVVTSLVRR
                     LQERTAEAEAADRQSKFARMLAERLAQETQPDIVLAAAAEMLQDYLEAPVCICWQAAG
                     DLQCVPQAAAMPDRDAVNWTMQNAKTLGAGSSNWPDSPDVMLPFSRLPGQSPVLVCRL
                     PRPDAERRQPELRSLCDQIALAYARASSAQQAQQAELNARAEAMQNALLASVSHDMRT
                     PLTAIIGAVSALDSQDAMLDESSRAQLLQSIRGEAEHLCRAVENILSLARLDALGSRG
                     LTLDWQSPEEIIGSTLARYRIRPRPCRLASAISSQALIRADAVLLSQALANLLDNAIA
                     VHQGPDPILLSASQEGETLVIAVADHGPGFPADFQVADIQKFRSGSTQGFGLGLIIVS
                     TIAALHDAQLVCRQRASGGAEVQLRFPVQRPDGDD"
     gene            complement(8087..>10139)
                     /locus_tag="VN084_07265"
     CDS             complement(8087..>10139)
                     /locus_tag="VN084_07265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWT29295.1"
                     /translation="EVSVNYASHRARIRWDDHRIQLSTILTEIRKLGYHAHPFSAEQQ
                     DALRQRQRKADFRRLAVAGLAAGQVMMIAVALYAGPAQGLEFATAQTLRWFSLVLTVP
                     AMTYCAWPFFRAAWRGLANRRIGMDVPISLGLLTGFVGSVWHTLNGAGTVYYDTLTML
                     IFFLLGTRYLERNARERSIEAAENLLRLVPAIVTRLEAGQATLVPLVEIDDDDLLLVK
                     PGETIAADGLVAEGESSADESLLTGESQPVAKRAGSEVYAGSINYESPLTIRVTATGE
                     RTVLAGISRLLDRAQAEKPRLAQVADKVAAYFTYALLAAVVVIGLVWWQLAPERTLEV
                     VLAVLVVSCPCALSLAAPAAFAAAGSHLVGRGVLLTRGHALEALAKVTHVVIDKTGTL
                     TLGRPALLKTIPLAELPAQACLQIAASLEQHSEHPLARSFLEAMQDAPMLAVDGVVNH
                     PGQGVSGQVAGHTYRLGNARLVEAEMPDLSADDLPASATIVLLRDTERLLAAFVLDDK
                     PRPQAAELIRELHAAGVAVSIFSGDAEPVVRHFAQAVGISQWRAGMSPEQKLAALEAL
                     QAQGQIVAMVGDGINDAPVLAKAQISIAMGSGTQMARTTADVVLLSEHLLEIRHAMKT
                     SRFGIRVIRQNFAWALIYNGIALPFAAAGLLAPWMAAIGMSVSSLVVVVNALRLRQTQ
                     A"
BASE COUNT         2062 a         3239 c         3094 g         1744 t
ORIGIN      
        1 ctggacgctg aaggcaaggc gacgccagcg ctgctgaaga aactcgccgc gctcggcgcg
       61 gatgaaggcg ccgtcgctac gctcaagcgc gagaacgacg gcaaggccga catcctgttc
      121 ctcgatgcca tgatccaggg cgcgacgctg caggaaggct tgcagaaagc gctggacgaa
      181 gccctggcca agctgccgat tcccaaggtc atgacgtacc agttagccga tgggtggagc
      241 aacgtcaact tcgtgcgccc tgcccatggg ctggtcgccc tgcacggcag cgatatcgtc
      301 gcaatcgaaa ccctgggcct gaaggccggc aaaacgacac aaggccatcg cttcgaggcg
      361 aagcacccaa tcgtcaccct caaggatgcc gacagctatg ccgcacagct gaaggacgaa
      421 ggtgccgtca tcgccagctt cgcagagcgc cgcgccgaga tcgtgcgcca gctctccgct
      481 gcggccgcaa aggagaacct caagccgatc gatgatgacg ccctgctcga tgaagtcact
      541 gcgctggtcg aacggcccaa cgtattgctc ggccagttcg agcaggaatt cctcgaagtg
      601 ccgcaggaat gcctgatcct gaccatgaag gccaaccaga aatatttccc gctgctcgac
      661 agcaagggca agctcaccaa caaattcctc atcgtcagca acatcgcgcc aagtgacgcc
      721 agtgccgtca tcggcggcaa cgagcgcgtg gtccgcccac ggctcgcaga tgccaagttc
      781 ttcttcgacc aggaccgcaa gaagtcactc gcatcgcgcg tgccaggcct ggccaaagtg
      841 gtctaccaca acaagctagg cacccagggc gagcgcaccg aacgcgtacg caagatcgcg
      901 ggctgggttg cgcagaagtt gggtgccgac cccaagctgg ccgaccgcgc agccgagctg
      961 gccaaggctg acctgctgac cgacatggtc ggcgagttcc cggaactgca aggcatcatg
     1021 ggcggctact atgcccgcca cgatggcgag aacgagctgg tcgccgctgc catcgccgat
     1081 caatatgtcg tgaagcgcga cgatgaagaa agcgcgtcca acctggtctc cgaatccctg
     1141 ctcatcgctg atcgcgtcga gactctggtc ggcatctggg cgatcggcct caagcccacg
     1201 ggcgagaagg atccattcgc cctgcgccgc catgcgctcg gcgtcatccg ctccttcgag
     1261 ctatgccatg cggccgcacg tatcggcaaa caagccttcg cgctcaagct ttccgagttg
     1321 ctggcctatg ccgcaagcac tttcgatagc gtcaagatcg agcccgagat actggccgag
     1381 atccatagtt tcatctatga acgctatcgc aatcagctgg ctgccgatta caaaccgcag
     1441 acgctggatg ccgtgatcag cctcaatccg gagctacatg aagtgatcat tcgttcagaa
     1501 gcggtcaagg ccttccagca gctgtccgaa tcggagagcc tcgccgcagc caataagcgg
     1561 gtcggcaaca tcctgaagaa agccgacgag accgttgcgc tgactgtggt cgatcacttg
     1621 ctcaaggagc cagccgaagc cgcattggca caggccctgt ccaaggtcaa accgcaggcg
     1681 gaccaggcct ttgaggctgg cgactatgcg ggttcgctga aatccctcgc cgtacttaaa
     1741 gcgccggtgg acgatttctt cgacaacgtc atggtcaacg ccgaagaccc tgccttgcgc
     1801 gccaatcgcc tggccctgct ctcccagcta catgccgcga tgaatcgcgt ggctgactta
     1861 tccaagctag cggcgtaggt ccagtgattt tcagcctgat agtgagatga cgcaatgaaa
     1921 ttagtgatcc tcgaccgcga tggcgtcatc aactacgaca gcgcgcaatt catcaagagc
     1981 ccggacgaat ggaaacccat tcccggcagc cttgaggcga tcgcgctgct taaccagagc
     2041 ggttatcgcg ttgcgctggc cacgaaccag tccggcatcg gccgcggcct gttcgacatg
     2101 gcgacattga acgccatcca cgacaagatg caccgcgcgt tggcgcaact tggcggccgc
     2161 atcgacgccc tgttctattg cccgcacgcg gccgacgcca agtgcgactg ccgcaaaccc
     2221 aagcccggca tgatcgagga gatcggccgc cgcttcgccg tcgatctcgc gggcgtgccc
     2281 agcgtcggcg actccctgcg tgacctggaa gccagcgccg ccctcggcgc gcaacctatt
     2341 ctcgtgaaaa caggcaaggg cgagtgcacc atcaacaacg gcggcctgcc ggcagggacc
     2401 tggatctgtg acgacctggc ccaggctgtg cagcggatca tcgccgagga caagccggtg
     2461 gcggaaaagc acgcgcgcga caaatcgccg gcgaaagcgg tcaaggacaa aagctccaaa
     2521 gaaagccggt gatgattgtc ttgcggtcct tgctcttcac cctgggacag tggctgttca
     2581 cgccactgtt cacgctgttc gccatcctga ccttcccgtt caagccggtc acccgctatc
     2641 gtttcatctc tcactgggcg cggatcatgc tctggtggtt gcgcgtcact tgtggcgtgc
     2701 gttatcgcgt catcggccgc gaacatatcc cgccacacgc gtccgtcatc ctctccaagc
     2761 atcagtcagc ctgggagacg ctcgccttcc aggagatctt cccgccgcag gtgtgggtgc
     2821 tgaagcgcga actcttgtgg gtcccctttt tcggctgggg tctggcgatg acctcgccga
     2881 tcgccatcga ccgcagcgcc gggcgcgagg ccctgaagca gatggtggct cagggcaggg
     2941 atcgtttgca gcaaggcttc tgggtggtga tcttccccga aggtacccgt atcgcgccgg
     3001 gcaagaaagg caaataccat atcggtggcg cctggctggc gacacacacg gcttcgaccg
     3061 tcgtccccgt cgcgcataac gctggggaat tctggccgaa gaacgctttc atcaagcgcc
     3121 cggggaccat cacggtcagt atcggcccgc ccatcgacag tagcggcctc aaggcggatg
     3181 aactcaatca gcgtgtggag cgatggatcg aatcacaaat gccgctgctg gaacacacat
     3241 gagcgagcat ctgctgacgc tggcggatgg cgcgcagatc ccgtatctgc ttgagcgccg
     3301 gccacgccgt accgtcggca tgcgtatcgg cgcgcaaggc ctggtggtgc atgcaccgac
     3361 cagactctcg ttgagtcatc tcgaaaccct gctgctgggc aaggctcaat ggatcctgaa
     3421 caagctacag ctgcacgcga cccatgcggt ggcgcctttc cagtggcgcg atggcgacaa
     3481 tctgctgtgg ctggggaacg agatgctgct gagcgtccgg cacgatagcc gcagccgcgc
     3541 catcatgcac gaacccggtc agctgcatgt cgccttgcct accccacatg acgcggccac
     3601 cgtcagtcgc aaggtgctac tgtggtatcg caagcaggca caagccgatt tcagccgccg
     3661 tttgcagatc ctggccgcac gcctgggcgt cgacacgccg ccactctacc tatcgaacgc
     3721 gcgttcgcgc tggggcagct gtaattcacg cggggagatc cgcatcaact ggcgcctgat
     3781 ccaggcgccg ccccatatca tcaactacgt catctgccac gaactggcgc atctcaagga
     3841 aatgaaccat tcggcccgat tctgggcggt ggtcgcacaa ctgcatcccg attacaaacg
     3901 ggccgagcag gaactcaaag ccctgtcggc gcgactgcat cagctagata tacactcttg
     3961 accccagctc gatgacgctg ttgatcggga tattgaaata ctcgacgaca ctgcccgcgt
     4021 tgcgcgacat ggccgcaaac agactgtcgc gccatttcgc catgcggcca ccagcgctcg
     4081 gcacgacaac ctcgcgactg acgaagtacg acgtcgtgaa cggatcgatg acgacgcccg
     4141 gcaaagggca ttgcgccaat gctttcggga tatccggatt ctccatgaag ccgtaacgca
     4201 aggtcatctg ccagaaccca tggcccaggg cctggtgatg cgatcgtgcg ttcgacgcga
     4261 ccgtgggggt atcggcatat tccaccgtca ggaccagatt ggtccggtgc agcacctgat
     4321 tgtgcttgag gttatgcatc agggcctgtg gcgcgctatg cggatcggac acaaggaata
     4381 ccgcagtgcg ctcgatgcgc ggcagtgtgc tgtctgccat gctggacacg aagttttgca
     4441 gcagcgggtc gtcctgcctg atcgatttca gcaacgcatc gcggccctgc ttccatgtcc
     4501 acatcaggag caacatcccc gcagccacga ccactgggaa ccagccgccc tgcaggaatt
     4561 tgaccgaaca cgagaccagc agcagactat ccagggcaac gaaacagccg gtcgcacccg
     4621 ccgccagcca cagcggatac cgccatccgt agcgaagcac gaagaaggtc aggatcgtcg
     4681 tgatgagcat ggtgcccgtg acggctatgc catatgccgt agccagcgct gacgagtttc
     4741 cgaagctgac ggtgactgtc agcaccgcca ccagcaataa ccagttcacc gcaggcaagt
     4801 agatctggcc ggactcggtc gctgaagtat gcacgatgcg catccgcggc aagaacccca
     4861 actggatcgc ctgacgcgtg atggaatagg cgccggagat caccgcctgc gaggcgatga
     4921 tcgtcgccat ggtggccagg atgatggcag gcaccagcag ggctggcggg aaagacaggt
     4981 agaagggatt gcttacggcg gcgggatgat cgagcaacaa ggcgccctgc cccatgtagt
     5041 tcagggccag ccccggcaat accaagccat tccaagcgat ccggatggcc ggcttgccga
     5101 aatgccccat atccgcgtat agcgcttccg caccagtgat cgccagtacg acggcgccca
     5161 cagcgagaaa cacgccactc ccgcgtgcca tgaggaaggt cagcgcgtgc atggggttca
     5221 acgcggcaag tatggcgggg gcgtggacga tgtgccagag accgatcgcc cccagcacca
     5281 ggaaccagac gatgatgacc gggccgaaca acttgcccac cgcctgtgtg ccaaagcgct
     5341 gtacggcgaa caggccgaac aggatcgccg ccgacaacgg catgacgaag ctcgccaggc
     5401 ccgggctcac gacctgcagc ccttcgatcg ccgacaacac cgagatcgcc ggcgtcagca
     5461 cgctatcgcc gtaaaagagc gcgcccccca tgaccccgag taaaagcagc catggccgat
     5521 gctgctgttt cgccttggtg accgcaagcg ccagtagcgc catgacgccg ccttcgccgt
     5581 gattgttcgc gcgcaggaca aggatcacgt atttgagcgt aaccaccagc atcagcgccc
     5641 agaacacagt ggatacggca ccgacaagat tggcaggcgt gagcgggatg ccggtcgatg
     5701 cgctgaagat ttcgcgaatg gtatacagcg ggctcgttcc gatatcgcca tacaccaccc
     5761 cgatcgcacc cagcgtcagt gcggcggtcg aacttccatg ttgtgctttc ataccggcct
     5821 cgtaagatga agatgaggcc attaggcaat agtcaacgta tcaaacgcat aaacgcacca
     5881 aagtcattct cattgattcg ccaggcgata gccgatgccg agttccgtca gcaggtagcg
     5941 tggctggccc gggtcggctt ccagtttggc gcgcaatcgg cccatatgga tacgcaggta
     6001 atgcgtgtca tcgacatact ccgggcccca caccgtcgcc agtaattcac gatggggata
     6061 gatacgtccg gggcgctgcg ccagcgacat cagcagcttg tgttccgtct tgctgagatg
     6121 cacagggtgt ccctccagcg tcacggtgcc gctaccacga tcgatgcgca gaccgccgac
     6181 ctcatatacc tgatcgcgca gtgtcatcag gctcacatgg cgcaacgcga cacgtacccg
     6241 tgccagcaat tcctccacgc cgaagggctt ttgcagataa tcatccgccc ccaggttcag
     6301 gctatcgacc ttttgtcgct cggactggcg tgcagagagc acgaggatcg ggatatccga
     6361 atgtttgcgc aaactgcgga tgaagtcggc gccgtcaccg tccggcaggc caagatcgag
     6421 gatcaccata ttcggcgtca gggacaccat ctgatgcagg gcttcagcca gcgtccgtgc
     6481 cgatacgggc ttgaatccgg cagcctgaag ggatgcagcc agaaagcggc tgatctcacg
     6541 gtcgtcctcg accaccagga ccgtggcgtt gtcagtcatc gccatctggt cgctggacgg
     6601 ggaaacgcaa ctggacttct gccccgcctg aggctcgttg ccggcagacg agctgtgcat
     6661 catgcagggc cgcgatcgtg gagacgatga tcagacccag cccaaaaccc tgggtcgatc
     6721 ctgaacggaa cttctgtatg tccgccacct ggaaatcggc ggggaatccc gggccgtggt
     6781 ctgcgaccgc aatgaccaat gtttcgccct cctggctcgc gctgagcaag atgggatccg
     6841 ggccctgatg cacggcgatg gcgttatcca gcaggttggc cagcgcctgc gacaagagga
     6901 cggcgtcagc ccgtatcaat gcctggctac tgatggccga tgccagccga catggtcgcg
     6961 ggcgtatccg ataccgtgcc agcgtactgc caatgatctc ttccggggat tgccagtcaa
     7021 gcgtcagacc ccggctgccc aaggcatcaa gtcgcgccag ggacaggatg ttctcgacgg
     7081 cgcgacacaa atgctcggct tcgccgcgga tggactgcaa cagctgtgcg cgactactct
     7141 catcgagcat cgcgtcctga ctgtcgagcg cactcaccgc accaataatt gccgtcagcg
     7201 gcgtgcgcat gtcatgggag accgaggcca gcaaggcgtt ctgcatggct tccgcgcgcg
     7261 cgttaagttc tgcctgctgg gcctgctgcg cgctactggc ccgcgcgtag gcaagggcga
     7321 tctggtcgca caggcttcgc aactcaggtt gccgtcgttc tgcatctggc cggggcaaac
     7381 gacagaccag caccggagac tgtccgggaa gccgactgaa agggagcatg acatcgggcg
     7441 aatccggcca gttgctgctg cctgcgccga gcgtcttggc gttctgcatc gtccaattca
     7501 ccgcatcgcg atcaggcatg gcggcggctt gcggcacaca ctgcagatca ccagctgcct
     7561 gccagcagat acacaccggg gcctcaagat agtcctgcag catctccgct gctgcggcca
     7621 gcacgatgtc gggttgtgtc tcctgtgcca gtcgctctgc cagcatgcgt gcgaacttcg
     7681 attgccggtc ggcagcctcg gcttcggccg tgcgctcctg caggcgcctg accagcgaag
     7741 tcaccaccag cgataccgtc agaaagccgc cgagggcgac ccaggattcg acgctggcga
     7801 tccggaacgt gtatcgcggt tcgacgacca ggaaattgat gagcaaggtg gcgcacaagg
     7861 ccagcgataa cgcgagggtg acctgcagat aataagcgcc ggccagcacc accagcaggt
     7921 agagcgtcac cgtgcccgcc atgccgaggg tgtcctgatt cagataaacg acagcgctga
     7981 cgacgatggt caggactgcg atggctagca atgcgaaaga tggacgcatg accgggtcat
     8041 gataacgcga tgcgtgtatc aatcgcgtaa aggatggaag aggcgcctaa gcctgcgtct
     8101 gccgcagccg taacgcattg acgacgacca ccagcgaact cactgacatg ccgatggcag
     8161 ccatccaggg cgcgagcaac cctgccgccg cgaacggcag cgcgatcccg ttatagatca
     8221 gcgcccaggc aaagttctgg cggatgacac ggatgccgaa gcggctggtc ttcatcgcgt
     8281 gacggatctc cagcaggtgc tcgctgagca gcaccacatc ggccgtggta cgtgccatct
     8341 gcgtgcccga gcccatggcg atggagatct gggccttggc cagcacgggc gcgtcattga
     8401 tgccgtcgcc gaccatggcg acgatctggc cttgcgcctg cagggcttcc agtgctgcga
     8461 gtttctgttc cggcgacatg ccggcgcgcc attggctgat gccgacggct tgcgcgaaat
     8521 ggcgtaccac cggttcggca tccccactga agatcgacac cgcgacgccc gccgcatgca
     8581 gttcgcggat gagctctgcc gcttgcggac gcggtttgtc gtcgagcacg aacgccgcca
     8641 gcaggcgctc tgtgtcgcgc aatagcacaa tggtggcact ggcgggaaga tcgtccgccg
     8701 acaggtccgg catttcggcc tcgaccaggc gcgcattgcc gagccgataa gtgtggcctg
     8761 cgacctggcc ggagacgccc tgccccggat ggttgactac gccgtcgacc gcaagcatcg
     8821 gtgcatcctg catcgcttcc aggaaactgc gcgcgagcgg gtgctcggag tgctgttcca
     8881 gactggcggc gatctgcagg catgcctgtg cgggcaactc tgccagcggg atcgttttga
     8941 gtagcgccgg gcgtcccagg gtcagggtgc cggtcttgtc gatcaccaca tgtgtgacct
     9001 ttgccaacgc ttcaagcgcg tgaccgcgcg tgagcagcac gccgcgaccg acgagatgcg
     9061 acccggccgc ggcaaaagct gccggcgccg ctagtgacaa ggcgcagggg cagctcacca
     9121 ccagcaccgc cagcacaacc tccagcgtcc gttcgggcgc gagctgccac cacaccaggc
     9181 caatcacgac cacggctgcg agcaaggcat aggtgaaata ggcggcgacc ttgtccgcca
     9241 cttgcgcgag ccgaggtttc tccgcttgcg cccgatcgag cagccgcgag atccccgcca
     9301 gtacggtacg ctcgccagtg gccgtgacac ggatggtgag cgggctctcg taattgatgc
     9361 tgccggcata gacttcactg ccggctcgct tggcgactgg ctggctctcg cccgtcagca
     9421 acgattcgtc ggcgctgctc tcgccttcgg ccacaagccc gtcagcggcg atggtctcgc
     9481 ccggcttgac caggagcagg tcgtcgtcgt caatctcgac cagcggcacc agtgtcgctt
     9541 gccctgcctc caggcgggtg acgatggccg gcaccaggcg caacaggttc tcagccgctt
     9601 cgatggacct ctcgcgcgcg ttacgttcca ggtagcgcgt gccaagcagg aagaagatga
     9661 gcatggtgag cgtgtcgtaa tacaccgtgc cggcaccatt gagcgtatgc cagacactgc
     9721 cgacgaagcc cgtgagcagt cccaggctga tcggcacgtc catgccgata cgacggttgg
     9781 cgagcccccg ccaggcggcc ctgaagaacg gccaggcgca gtaggtcatg gcaggcaccg
     9841 tcagcaccag gctgaaccag cgcaaggtct gcgccgtggc aaactccagc ccctgcgccg
     9901 ggccggcata gagcgcgacg gcgatcatca tgacctgccc ggctgcaagc ccggcgacgg
     9961 cgaggcgacg gaaatcggct ttgcgctggc gctggcgcag ggcgtcctgc tgttcggcgc
    10021 tgaatgggtg ggcgtggtag cccagcttgc ggatctcggt gaggatggtg ctgagctgga
    10081 tacgatggtc gtcccagcgg atgcgtgcgc gatgcgacgc ataattcacc gagacttcg
//
LOCUS       DATMTW010000372         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_99064, whole genome shotgun sequence.
ACCESSION   DATMTW010000372 DATMTW010000000
VERSION     DATMTW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99064"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /gene="ttcA"
                     /locus_tag="VN084_07270"
     CDS             complement(<1..863)
                     /gene="ttcA"
                     /locus_tag="VN084_07270"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWT29296.1"
                     /translation="MKHYPDDASGNFLKLRDRLISATGKAIGDYNMIEQGDTILVCMS
                     GGKDSHAMLMLLLALQERAPVDFKLIAMNLDQKQPGFPEDILPEYLKKLGVDYRIVEA
                     DTYSIVKEKIPEGKTTCSLCSRLRRGVIYTTAKELGANKIALGHHRDDMVETLFLNMF
                     FGAKLKAMPPKLSANDGQLIVIRPLAYCAEKDIAAYARGMAFPIIPCDLCGSQENLQR
                     QRVKEMLQVWEREQPGRINNIFRAMANVEPSHLADTDLYDFKGMSQALATDEDPLFGD
                     IAKEDNTALSLA"
     gene            complement(984..1736)
                     /locus_tag="VN084_07275"
     CDS             complement(984..1736)
                     /locus_tag="VN084_07275"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HWT29297.1"
                     /translation="MNSGLQDKVVLITGGAKRVGASICRLLHAGGANLMIHYRSSVGE
                     ARALQSELNLLRPNSAAIIQGDLLNLSILPSLVQETIHHFGKLDVLINNASSYYPTEI
                     GEIDATQWEDLIGSNLKAPLFLAQAAAAELRKQHGNIVNITDMHVERPKRGYIVYSVA
                     KAGLMTLTKSLALDLAPDVRVNAVAPGPVLWPEDNPQFDEVYRQRVISQTLLKRIGEG
                     DDVAKAVRFLIQDAPFITGQVIAVDGGRSLNL"
     gene            1772..2920
                     /locus_tag="VN084_07280"
     CDS             1772..2920
                     /locus_tag="VN084_07280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWT29298.1"
                     /translation="MTPLPIPGSEALRHSQSLVAHIREEIARSGGWIDFAHYMQLALY
                     APGLGYYSGGAEKFGAAGDFVTAPELSPLFAQCLARQAAQVCAQTKGDILELGAGTGR
                     LAVELLLELQRLDQLPAHYLILEVSAYLQDLQRQTLMAQLPPDLFARITWLDHLPSGF
                     DGLVLANEVLDALPVHIVGQREHAFERGVSFEGDALVWSDRHPLPPTLNALAAPLALA
                     AGYLTELCPLAGALVASLAQSLRRGVLLFIDYGFPRREYYHPQRDHGTLMCHYRHHAH
                     GDPFLYPGLQDITAHVDFTAAAEAGVANGLRLAGYTTQAQFLINCGITELLGQIAAGS
                     AAYLRLASQAQKLLSPAEMGELFKVLAMSKGQDAPLLGFVRGDKSHAL"
     gene            2975..3676
                     /gene="trmB"
                     /locus_tag="VN084_07285"
     CDS             2975..3676
                     /gene="trmB"
                     /locus_tag="VN084_07285"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HWT29299.1"
                     /translation="MSMQDQDNNVKHRPIRSFVLRQGRLTDAQQRALETGMPLFGVPY
                     DPAAPLDLAALFGRSDSLKILEIGFGMGESTARIAQAQPERDFLAVEVHTPGVGSLLK
                     QIQEAGLSNLRIIQHDAVEVLDHMIADASLDGVHIFFPDPWHKKRHHKRRLIQGEFVR
                     RLCRKLKVGAYLHVATDWQEYAEWVLEVLQAEPALANTAEKYAEKPDYRPLTKFENRG
                     IKLGHGVWDIIFRRR"
     gene            complement(3748..>3872)
                     /locus_tag="VN084_07290"
     CDS             complement(3748..>3872)
                     /locus_tag="VN084_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HWT29300.1"
                     /translation="TIWGNCSVQLFSSLKKQGVEEAESVIGKWLFQAESDDQPD"
BASE COUNT          737 a         1132 c         1179 g          824 t
ORIGIN      
        1 gccaaactca aagctgtgtt gtcttccttg gcgatgtctc cgaacaacgg gtcttcatcc
       61 gtcgccagcg cctggctcat gcccttgaaa tcatagaggt cggtatccgc cagatgcgag
      121 ggttcgacat tggccatggc acgaaagatg ttgttgatac ggccgggttg ctcgcgctcc
      181 cagacctgca gcatctcttt caccctttgt cgctgcaggt tctcctgcga gccgcagaga
      241 tcgcagggaa tgatggggaa cgccatcccg cgggcgtagg cagcgatatc cttttcagcg
      301 cagtaagcca gcgggcggat gacaatcaac tgaccgtcgt tggcggagag cttgggcggc
      361 atggccttga gcttggcacc aaagaacatg ttgaggaaca gcgtctctac catgtcgtcg
      421 cggtgatggc caagggcgat cttgttggca ccgagctcct tggccgtggt gtagatgacg
      481 ccgcggcgca gacgtgagca cagagaacaa gtggtcttgc cctcggggat cttctccttg
      541 acgatggagt aggtgtcggc ttcgacgatg cggtaatcca cgcccagctt cttcaggtat
      601 tccggcagaa tatcctccgg gaaccccggc tgcttctggt cgaggttcat ggcgatcagc
      661 ttgaagtcga ccggtgcgcg ctcctgcaag gccagcagca acatgagcat ggcatgcgaa
      721 tccttgccgc cgctcatgca caccaggatc gtgtcaccct gttcgatcat gttgtagtcg
      781 ccgatggcct tgccagtggc gctgatcagg cggtcacgca gcttgaggaa attgccggag
      841 gcatcgtctg ggtagtgttt catctgagca tcattcctac tacggatgga cacggatcaa
      901 cagggattgg aaccgggaat gcgactgcgc agttttcgcg gcggtgcatc cgtatttatc
      961 catgatcatc cgtggttaaa gccttatagg ttaagtgaac ggccgccgtc gacggcgatg
     1021 acctggccgg tgatgaacgg cgcgtcctgg atcaggaaac gcactgcctt ggcgacatcg
     1081 tccccttcgc cgatacgttt gagcagggtc tgcgagatga cgcgctgacg atacacctcg
     1141 tcgaattgcg gattgtcctc cggccacagc accggtcccg gcgcgacggc attgacgcgg
     1201 acgtcaggcg ccagatccag cgccagcgat ttggttaacg tcatgaggcc ggccttggcc
     1261 acgctgtaga cgatgtaacc gcgcttgggg cgttcgacgt gcatgtcggt gatgttgacg
     1321 atgttcccat gctgcttgcg caattctgcc gccgcggctt gcgcgaggaa caatggtgcc
     1381 ttgaggttgg agccgatgag gtcttcccat tgcgtagcgt cgatctcgcc gatctcggtc
     1441 ggataatagc tggaggcgtt gttgatgagc acgtcgagct tgccgaaatg gtggatcgtt
     1501 tcctgcacca ggcttggcag gatgctgaga ttgagcaggt ccccctggat gatggccgcg
     1561 gaattcggcc gcagcaggtt gagttcggat tgcagggcgc gcgcctctcc caccgaactt
     1621 cgataatgga tcatcaggtt cgccccgccc gcatgcagca gacggcagat agaagcgcca
     1681 acgcgtttcg cgccgccggt gatcaatacg actttgtctt gcagaccaga attcatgcgg
     1741 ggttccatcc aggcattata atccattcaa tatgacacca ttgcccattc caggctcgga
     1801 agcgctgcgc catagccaat cccttgtagc gcatatccgc gaagagatcg cccgttctgg
     1861 aggctggatc gatttcgcgc attacatgca gcttgcgctc tatgcgccgg gccttgggta
     1921 ttacagcggc ggcgcggaaa agttcggggc cgccggagat ttcgtcacgg cgccagaact
     1981 gtcaccactg ttcgcgcagt gtctggctcg gcaggcggcg caggtctgcg cacagaccaa
     2041 gggcgatatc ctagagctgg gcgccggtac cggacgactg gccgtcgagc tcctgctgga
     2101 gctgcaacga ctcgatcagc taccggcgca ttacctgatc ctggaagtga gcgcatattt
     2161 acaggatctc cagcgccaga ccttgatggc acagctgccg cccgacctgt tcgccaggat
     2221 cacctggctc gaccacttgc cgtccggctt tgatggtctg gtgctggcca atgaggttct
     2281 ggatgccttg ccggtgcata tcgtcggcca gcgagaacat gcgttcgagc gcggtgtatc
     2341 gtttgaaggt gacgctctgg tctggtcaga ccggcatccg ctgccgccga cattgaatgc
     2401 gctggctgcg ccattagcct tggcggccgg ctacctgacc gagctttgcc cattggccgg
     2461 cgcgctcgtc gcgagtcttg cccaaagtct gcgaaggggc gtgctgctgt tcatcgatta
     2521 tggattcccg cgccgcgagt actatcatcc acagcgggac catggcacct tgatgtgtca
     2581 ttacagacac cacgcgcatg gcgatccgtt cctctatcca ggtctgcaag acatcacggc
     2641 acatgtcgat ttcacggccg cagcggaagc cggcgtcgcc aatggcctgc gcctcgctgg
     2701 ctacaccacg caggcgcagt tcctcatcaa ctgcggcatc acagagcttc ttgggcaaat
     2761 cgcggccggg agcgcggctt acctgcggct ggcgtcgcaa gcgcagaagt tattgtcgcc
     2821 ggccgagatg ggtgaactgt ttaaggtgct cgccatgagc aagggccagg acgcgccgct
     2881 gctcgggttt gtgcgtggcg ataaatcaca tgcgctgtaa gcccgcaccc cgcgggacga
     2941 gtccgctata atcgcgcgtt cctcattcga agccatgtcc atgcaagacc aagacaacaa
     3001 cgtcaagcac cgcccgatca gaagcttcgt gctgcgtcag gggcgtctga ccgatgcgca
     3061 gcagcgtgcg ctggagaccg gtatgccatt gttcggcgta ccttatgatc cggccgcgcc
     3121 actggatctc gccgccttgt tcggcaggtc cgacagcctc aagatcctgg agatcggttt
     3181 cggcatgggc gagagcacgg ccaggatcgc gcaagcccag ccggagcggg attttctggc
     3241 agtggaagtg cataccccag gcgtaggtag tctactcaag cagatccagg aggccggcct
     3301 cagtaacctg cgcatcatcc agcacgatgc ggtggaagtg ctcgaccaca tgatcgcgga
     3361 tgccagtctt gatggcgtgc atatcttctt cccagatccc tggcacaaga aacgccacca
     3421 caagcgtcgc ctgatccagg gcgagttcgt ccggcggctg tgccgcaagc tgaaagtggg
     3481 tgcttacctg catgtcgcaa ctgactggca ggaatatgcg gaatgggtac tggaagtgtt
     3541 gcaggccgag ccagcgctgg ccaacaccgc cgagaagtat gccgaaaagc cggattaccg
     3601 accgctgacc aaattcgaga accgcggtat caaactaggc cacggcgtct gggatatcat
     3661 ctttcgccgc aggtgattgc tggcgcgcat catgccttgt cgcagtgagg gctcttgccc
     3721 attgcgactt ttctcgcgcc tgtcgcttca atccggttga tcgtcgcttt cggcctggaa
     3781 cagccatttg ccgatgacgg attctgcttc ttccacgcct tgcttcttga ggctggaaaa
     3841 gagttgcacc gagcagttgc cccagatcgt ct
//
LOCUS       DATMTW010000373         5117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_199926, whole genome shotgun sequence.
ACCESSION   DATMTW010000373 DATMTW010000000
VERSION     DATMTW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199926"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1251
                     /locus_tag="VN084_07295"
     CDS             46..1251
                     /locus_tag="VN084_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWT29301.1"
                     /translation="MPTYNYKAVDQKGLPAQGQLDASNDIDLELRLDRIGLSLLTFRE
                     AKKSAPVFNTNKVGLQDLVMFCFQLEQLSSAGVPLLEGLTDVRESTLNPYFQKIIGAV
                     VSEVEGGKMLSQALAEHPRVFSDVFVSLVRAGEQTGKLPQVFENLATTLKWQHELISQ
                     TKRLIAYPLFVLFVVLGAVVFLMIYLVPQMVSFLGNLGQELPTQTKVLIFISGVFVDY
                     WWLLLALPIVAVLGVGIALKRSARFRYQFDYMMLHLPVTGPILHKIIMARFARYFALM
                     YETGIPVLDAVKTCQDIVANRVVADALERAHQQINAGDSMSESFRNAGLFPPLVVRMI
                     KVGENTGALDKSLANIGYFYDRDVNESIEKMLKLIEPALTVVLGGILAFIMFSVLGPV
                     YDSFSKLKL"
     gene            1254..2837
                     /locus_tag="VN084_07300"
     CDS             1254..2837
                     /locus_tag="VN084_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29302.1"
                     /translation="MLAKIILYATNDGLTAGLWQSGQCRESRMFACDEAGVQAFEQYL
                     QQHLNVSIYILCDSIEEDYRLESLPHTSGSSRQELLTRKLAQAYRNSPFRVAHFINRE
                     KTKRKDDRFLFAALNKEEFLQKWLACIERQQAPLVGVYLISMISQLIVRRHKIMDPHI
                     LLSEKLSSGFRQSYLHNGRLRISRLAPIPDSAQNQLNYFYNVETEKTRLYLVSQRFIT
                     RETPLTIVLPALADTVEAVKRSLEQEQGIECKVIDLAAFAKTLKLDPEAVRRNPELVH
                     MHLLATGNVPDNLAPASLIKNHQVNALRQWTNLASGLVLLIGLVLAALNFNQALDDEA
                     AISQAALETRVQEQRYAEVAKDFPATPLPAADLQQAVEIHHQLVEAARSPRMVMEAIG
                     RGLAQTPEIDVVRLHLMRSEDIDVKDTSALNATAPVAVSTQANAAPAGTLYQICFLNG
                     EIRAFTGDYRAALASVTRLVDKLKADPAIEQVSIAQEPVNLSSFSNLSGSTVDEQAAR
                     LQAAAFQIKIVFKPEPTSP"
     gene            2834..3454
                     /locus_tag="VN084_07305"
     CDS             2834..3454
                     /locus_tag="VN084_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29303.1"
                     /translation="MKLTPEDLQKLRLPAIILVVVVVAMALLVWLTEQYKTDAGGRLQ
                     IQQQKLAQAGQRFRTSGEEKQTIIKYLPEYQRLIDIGFIGQEKRIEWVDGLRRIHKAN
                     KLFNISYTIAPQEPYKLGMLPNLGQFTLNRSVMKLELNMLHEGDLLTLMEQLRTEQGS
                     PFIMRDCELTRVGTAKADSFVPNMLAKCEIDWLTLREPVTTGSPTP"
     gene            3451..3948
                     /locus_tag="VN084_07310"
     CDS             3451..3948
                     /locus_tag="VN084_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29304.1"
                     /translation="MTRRILLLLCLLFGAHTAMADMGRVFFTPAERARLDELRANAKS
                     VDISEQADPQAMIEVAPPSDVSVQGYVKRGDGKKSTVWVNGKPVQENASGAGVEVGKL
                     GNSNQVPITLPNSGKSLKLKAGQVYSPATDSVSEAAGNSAMQSDPAQAGKIGTQPAGE
                     RPASP"
     gene            4026..>5117
                     /locus_tag="VN084_07315"
     CDS             4026..>5117
                     /locus_tag="VN084_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29305.1"
                     /translation="MILIMFMLGLGVIAFMLKSVGTSTLEFQQKDRTTQALNGAKEAL
                     IAWAVSHSDYPGQLPWPDRNGDGNYDGGSDCATTSFSTSMFIGQLPISTSTNPCINYP
                     GMGSDFLDGAGNRLWYAVSRNLVRNYQSGTNPIINPGIIDSPTYSWMRVLDRNGNLIS
                     DRVPAVIIAPGLPLPNQDRAGVPDATEYLDSLQIGATVYSNQDDDSDNEDFIMAPDSA
                     HHPGSDGTYQAPYYFNDRLVYITIDELMAATEKRVAAEVKAALNSYRSTYGAYPYAAP
                     LGATLNYSCKDNVTAGFLPVDAPVAFSSPVSCSSNTNCSGMSFVNDISRVAYTNPFIN
                     WTGTTGACTSSGRTCTCTGTGSCTIFGVID"
BASE COUNT         1088 a         1565 c         1489 g          975 t
ORIGIN      
        1 cgtgatcgac ctgacaagcc gcctcaacgt gggcaaatag ccgggatgcc gacctacaat
       61 tacaaggcgg tcgaccagaa ggggctcccg gcccaagggc aactcgacgc ctcgaacgat
      121 atcgatctcg aactgcggct ggaccgcatc gggctcagcc tgctgacatt tcgtgaggcc
      181 aagaagagtg caccggtctt caataccaac aaggtcggct tgcaggatct ggtgatgttc
      241 tgcttccagc tggagcaact gagcagcgct ggcgtgccgt tgctggaagg cctgactgac
      301 gtgcgggaga gtacgctcaa tccgtatttc cagaagatca tcggcgccgt ggtgtccgag
      361 gtcgaaggcg gcaagatgct gtcgcaggcc ctggccgagc atccacgcgt gttcagcgat
      421 gtgttcgtca gcctggtgcg ggccggcgaa cagaccggca aactgcccca ggtgttcgag
      481 aatcttgcga ctacgctcaa gtggcaacac gaattgatct cgcagaccaa gcgcctgatc
      541 gcctatcccc tgttcgtgct gttcgtggtg ctgggcgcag tggtcttcct catgatctac
      601 ctggtgccgc agatggtaag tttcctcggc aacctcgggc aggagctgcc gacccagacc
      661 aaggtgctga tcttcatctc cggtgtcttc gtcgattact ggtggttgtt gctggcgctg
      721 ccgatcgtcg ctgttctcgg cgtcggcatc gcgctcaagc ggagcgcgcg tttccgttac
      781 cagttcgatt acatgatgct gcatctgccg gtcaccgggc ccattctgca caagatcatc
      841 atggcgcgtt tcgcacgcta tttcgcgctg atgtacgaga ccggcatccc cgtactcgat
      901 gccgtaaaga cttgccagga catcgtggcc aaccgagtgg tggccgatgc gctcgagcgt
      961 gcccaccagc agatcaacgc gggcgacagc atgagcgaga gtttccgcaa cgctggcctg
     1021 ttcccgccat tggtggtacg aatgatcaag gtgggcgaga acacgggcgc actcgataaa
     1081 tcgctggcca acatcggcta tttctatgat cgtgacgtca acgaatcgat cgagaagatg
     1141 ctcaagctca tcgagccggc attgacggtg gtgctgggcg gcatcctcgc cttcatcatg
     1201 ttctcggtcc tcggcccggt ctacgatagt ttcagcaagc tcaagctctg accatgctcg
     1261 ccaagatcat cctctacgcg accaacgacg gcctcacggc tggcctctgg cagtccggcc
     1321 agtgcaggga gagtcggatg ttcgcctgcg acgaggccgg ggtgcaggcc tttgagcaat
     1381 atctgcaaca gcatctgaat gtttccatct acatactgtg cgattccatc gaagaggatt
     1441 accggctgga atccttgccg catacctctg gaagctcgcg gcaggagttg ctcacccgca
     1501 aactggcgca ggcctaccgc aacagcccgt tccgcgtcgc gcatttcatc aatcgcgaaa
     1561 agaccaagcg caaggacgac cggttcctgt tcgcagcact gaacaaggaa gagttcctgc
     1621 agaaatggct cgcctgtata gaacgccagc aggcgccatt ggtcggtgta tacctgatct
     1681 ccatgatcag tcagctcatc gtgcggcgtc acaagatcat ggatccgcat atcctgctca
     1741 gcgagaagct gagttccggg ttccgtcaga gttacctgca caatggccgc ctgcgcatca
     1801 gccggctcgc gccgattcca gacagcgcgc aaaaccagct caattacttc tataacgtcg
     1861 agaccgaaaa gacccggctc tatctggtca gccagcggtt catcacgcgt gagacgccgc
     1921 tgaccatcgt gctgccggca ctggcagaca cggtcgaggc ggtgaagcgc agcctcgagc
     1981 aggagcaggg catcgaatgc aaggtgatcg atctcgccgc tttcgccaag accttgaagc
     2041 tggatccgga agccgtgcgc cgcaacccgg agctggtgca catgcacctg ctggcgacgg
     2101 gcaacgtgcc ggacaatctg gcgccggcat ccctgatcaa gaaccatcag gtcaacgcgc
     2161 tacgtcagtg gaccaatctg gccagcgggc tggtgttgct gatcgggctc gtgctcgcag
     2221 cgctcaattt caatcaggcg ctcgacgacg aagccgcgat cagccaggct gcgctggaga
     2281 cccgcgtgca ggaacagcgc tatgccgagg tggccaagga tttccccgct acccccctgc
     2341 cggctgccga tttgcagcag gcggttgaga tccatcatca gctggtagaa gcggcgcgat
     2401 cgccacgcat ggtgatggaa gccatcggcc gtggcctggc gcagacgccg gagatcgacg
     2461 ttgtgcgcct gcacctgatg cgcagcgaag atatcgacgt caaggacacc agcgcgctga
     2521 acgccaccgc gccagttgcc gtttccacgc aggctaatgc agcgcctgct ggcacgctct
     2581 atcagatctg cttcctaaac ggcgagatcc gcgctttcac gggcgattac cgcgctgcgc
     2641 tcgccagcgt gacgcggttg gtcgacaagc tcaaggctga ccctgcgatc gagcaggtca
     2701 gcatcgccca ggaaccggtc aacctcagtt cgttctccaa cttgagcggc agtacggtcg
     2761 acgagcaggc ggcgagactg caagccgccg ctttccagat caagatcgtg ttcaaaccgg
     2821 agccgacctc gccatgaagc tgacgccaga ggatctgcag aagctcaggc tacccgcgat
     2881 catcctggtg gtcgtggtgg tggcgatggc cttgctggtc tggctgaccg agcaatacaa
     2941 gaccgatgcc ggcggccgtc tgcaaatcca gcagcagaag ctcgcgcagg cgggtcagcg
     3001 cttccgcacc tccggcgaag agaaacagac catcatcaaa tacctgcccg aatatcagcg
     3061 actcatcgac atcggtttca tcggccagga aaagcgcatc gagtgggtcg atggcctgcg
     3121 ccgcatccac aaagccaaca agctgttcaa tatcagctac accatcgccc cgcaggagcc
     3181 ttataagctg ggcatgctgc cgaatctcgg ccaattcacc ctgaatcgct cagtgatgaa
     3241 gctggaactc aacatgctgc acgagggcga tctgctgacc ctgatggaac agcttcgcac
     3301 ggagcaaggc tcgccattta tcatgcgcga ttgtgagttg acccgggtcg gcactgccaa
     3361 ggcggatagt ttcgtgccga acatgctggc caaatgcgag atcgactggc tgacgctgcg
     3421 tgagcctgtg accacgggaa gccccacgcc atgacgcgcc gtatccttct gctgctttgt
     3481 ctcctgtttg gcgcgcatac cgccatggcc gacatggggc gcgtgttctt cacgccggcg
     3541 gagcgcgcca ggctcgatga actgcgcgca aacgccaaat cggtggatat atcggagcaa
     3601 gccgatccgc aagcgatgat cgaggtcgcg ccgccgagtg acgtatcggt gcaaggctac
     3661 gtcaagcgcg gcgacggcaa gaagagcacg gtctgggtca acggcaagcc agtgcaggag
     3721 aacgccagcg gcgcgggcgt cgaggtcggc aagctgggta attcgaacca ggtgccgatc
     3781 acgctgccga atagcggcaa atccctcaag ctgaaagccg gacaagtcta ctcaccagcg
     3841 accgacagcg tcagcgaagc ggccggcaat tcagccatgc agagcgaccc tgcgcaagca
     3901 ggcaagatcg gcacccagcc tgctggtgaa cgcccggcaa gcccctagct gccgtcatgc
     3961 gacgcccaga tacaatcgcg tcgactcgcc tgcgaaaggc gcgcaacagg caatatggtg
     4021 cagccttgat cctgatcatg ttcatgctcg gtttgggcgt gatcgccttc atgctcaaat
     4081 ccgtcggtac cagcacattg gagttccagc agaaggacag gacgacgcag gctttgaatg
     4141 gcgcaaagga agcattgatc gcttgggctg tcagtcatag cgattatcct ggccagttgc
     4201 catggcccga tcgcaatggc gacggcaatt acgatggcgg cagcgattgt gccaccacgt
     4261 cattttccac ctccatgttc atcggccagc taccgatcag cacgtcgacc aatccatgta
     4321 tcaattatcc gggtatggga agtgattttc tggacggcgc aggcaatcgc ttgtggtacg
     4381 cggtctccag gaacctggtg cgcaactatc aatccggcac caacccgatc attaatcctg
     4441 gcatcatcga ttcaccgaca tattcttgga tgcgcgtgct cgaccgcaac ggcaacctga
     4501 tctccgatcg cgtccctgcg gtcatcatcg cgccaggact gcccctgccc aaccaggatc
     4561 gcgctggcgt gccggatgcg acggaatatc tcgatagcct acagatcggc gcaaccgtct
     4621 attcgaacca ggacgatgac agcgacaacg aggatttcat catggcgccg gacagcgcgc
     4681 accacccggg ttcagacggc acctatcagg caccttatta tttcaacgac aggctggtct
     4741 atatcaccat cgacgagttg atggccgcca cagaaaaacg cgtcgcggcg gaagtgaaag
     4801 cggccttgaa cagttaccgt agcacttacg gcgcttatcc ctatgctgcg ccgctgggtg
     4861 cgacgctcaa ttacagctgc aaagataatg tcacggcggg attcctgccg gtggatgctc
     4921 ctgtcgcgtt cagttcgcca gtctcgtgca gctccaacac caattgcagc gggatgagtt
     4981 tcgtcaacga tatatccaga gtcgcctata caaatccctt catcaactgg accgggacca
     5041 ccggtgcctg cacgagctct ggccgaacct gcacctgcac gggcaccggt agctgcacca
     5101 tcttcggcgt gatcgac
//
LOCUS       DATMTW010000374         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_299244, whole genome shotgun sequence.
ACCESSION   DATMTW010000374 DATMTW010000000
VERSION     DATMTW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299244"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VN084_07320"
     CDS             <1..538
                     /locus_tag="VN084_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29306.1"
                     /translation="LLLLLGWLWRKRIGLLEIVIALSVVVFYIYASNFIDHLMTRFGG
                     NDPSLSFKMQGINTYLDAGVEEKFTGFGYGTDPCIGCHYQDIGVILNLVSRGGIIVTL
                     PLALILLRIFALHGIILAAIIVLIPAYCIMYFYEAPIWIFLLFAISSRQMLDKRVQPG
                     TRSTAPGAIAPAGMVAHH"
     gene            563..>1506
                     /locus_tag="VN084_07325"
     CDS             563..>1506
                     /locus_tag="VN084_07325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWT29307.1"
                     /translation="MTRTRVNEIDLLRFLAALSVVLFHYAYRGPAGGFSAMPYPLLAP
                     VAEYGFLGVELFFMISGFVVLMTAAASARLRDFCISRVVRLYPAFWTCCTITFLASIA
                     LAARPGVTFVQYAVNMTMVADLFRVPLLEGAYWSLLVEIRFYALVAALLVIGKIHQAQ
                     RYLVMWLLACIALALVALFPDAWLPAGALHAVRTLLIFDYAAYFIAGATCFLIWKQGA
                     AVSRLTVLAGAWGLALFQSEVIVRKVRAENAMSTLNGAIVIGILTLFFLVMLLIALGR
                     TGSIARYRWPLAGAISYPLYLLHQNIGYLVFNRFYPTV"
BASE COUNT          233 a          488 c          437 g          348 t
ORIGIN      
        1 gctgttgctg ctgctcggct ggctgtggcg caagcgcatc ggcttgctcg aaatcgtcat
       61 cgcactgtcc gtcgtcgttt tctacattta cgcctccaat ttcatcgacc acctgatgac
      121 ccgcttcggc ggcaacgatc ccagcctgtc gttcaagatg cagggcataa acacatacct
      181 ggatgccggc gtggaagaga aattcacggg gttcggctat ggaacggatc cttgcatcgg
      241 atgccactac caggacatcg gcgtcatcct gaacctggtc agccgcggcg gcattatcgt
      301 gacgttgccg ttggcgctga tcctgctgcg catattcgcc ctgcacggca tcatcctggc
      361 agcgatcatc gtcctgatcc ccgcatattg catcatgtat ttctacgagg ccccgatctg
      421 gatattcctc ctgtttgcga tctcgagcag acagatgctg gacaagcgcg tccaacccgg
      481 cacgcgaagt acggcaccgg gggccatcgc gcctgcgggc atggtcgcgc accattgaat
      541 ccgccgcctg gaggttcacg ccatgacacg caccagagtc aacgaaatcg atcttctgcg
      601 attcctggcg gcgctctccg tcgtcttatt tcactacgcc taccgtgggc cggccggcgg
      661 cttctcggcg atgccgtacc cgctgctggc gcccgtcgcc gagtatggct tcctcggggt
      721 cgaactgttt ttcatgatca gcgggttcgt cgtcctgatg accgcggccg ccagcgcgcg
      781 cctgcgcgac ttttgcattt cccgcgtcgt acggctttac cccgcgttct ggacgtgctg
      841 cacgattacc ttcctcgcca gcatcgcact ggccgcgcgg cccggcgtca cgttcgtcca
      901 gtacgccgtc aacatgacga tggtcgccga cctgttcagg gtgccgttgc tggaaggggc
      961 ttactggtcg ttgctggtcg agatccggtt ttatgcactg gtggccgcgc tcctggtcat
     1021 cggaaagatt caccaggcgc agcgctacct cgtcatgtgg ctgctggcat gtatcgcact
     1081 cgcactcgtc gcgctgttcc cggacgcgtg gctgccggca ggtgcgctgc atgccgtccg
     1141 caccctgctg atcttcgatt acgcggcgta tttcatcgcc ggtgccacct gcttcctgat
     1201 ctggaaacag ggtgcggccg tgagcaggct cacggtactc gcaggcgcct ggggcctcgc
     1261 cctcttccag tcggaagtta tcgtgcgcaa ggtgcgcgcc gagaacgcca tgtcgacctt
     1321 gaacggcgct atcgtcatcg gcatcctgac cctgttcttc ctggtgatgc tgctgatcgc
     1381 attgggccgg acgggctcga tcgcccgcta tcgctggccg ttggccgggg cgatcagcta
     1441 tccgctgtat ctgctgcatc agaacatcgg ctatctcgtg ttcaatcgct tctacccgac
     1501 tgtaag
//
LOCUS       DATMTW010000375         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_501376, whole genome shotgun sequence.
ACCESSION   DATMTW010000375 DATMTW010000000
VERSION     DATMTW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501376"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VN084_07330"
     CDS             <1..783
                     /locus_tag="VN084_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWT29308.1"
                     /translation="TIPSLKREDLQRFYTTYYGARNAVIAMIGDMDRAQAERLAERIS
                     SGLPSSPAVVPLPSVSYPEHAVEQRIPHPAKQSHILLGYPGVKRGDPDYFPLYVGNYV
                     LGGGGFVSRLTEEVREKRGLVYSVYSYFMPMAELGPFQIGLQTKREQAEQALKVVHEA
                     LDKFMKDGPTEAELKAAKQNITGGFPLRLDSNSKILDYLAVIGFYRLPLTYLDDFNKQ
                     VNAVTTAQIRDAFKRRLDTNKLVTIIVGGDTAGNDSKDNTAN"
     gene            817..1371
                     /gene="rsmD"
                     /locus_tag="VN084_07335"
     CDS             817..1371
                     /gene="rsmD"
                     /locus_tag="VN084_07335"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWT29309.1"
                     /translation="MASQKNKVRIGGGEWRSRLLAFPDVPGLRPTPDRVRQTLFNWLG
                     QDLHGLNCLDLFCGTGVMGFEALSRGAHSVTMVEKASQAYRALLENQRALGAENARIV
                     QMDALQFLAGNQQMFDVIFLDPPYQQGWLDKLLPVIAPHLASGGMVYAEAEFALADSD
                     DWHIHKQGRAGNVFFHLLKSAHHD"
     gene            1393..1878
                     /gene="coaD"
                     /locus_tag="VN084_07340"
     CDS             1393..1878
                     /gene="coaD"
                     /locus_tag="VN084_07340"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWT29310.1"
                     /translation="MRTKAIYPGTFDPITLGHEDVVRRAAHLFDEVVVAVAQSHSKRP
                     LFSLDERVALAAEVLADCPNVKVVGFTGLLMQFAREEGARVVIRGLRAVSDFEYEFQL
                     AGMNRHLYPEVETLFLTPAEQYMFISASLVREIAQLGGDIGQFVSPRVQERIADKLVA
                     R"
     gene            1885..2136
                     /locus_tag="VN084_07345"
     CDS             1885..2136
                     /locus_tag="VN084_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HWT29311.1"
                     /translation="MALMITDECINCDVCEPECPNGAISQGEEIYVIDPDLCTECVGH
                     FDRPQCQEVCPIDCIPLDPAVVESKEQLQQKYLRLIAAK"
     gene            2164..>2466
                     /gene="gloA"
                     /locus_tag="VN084_07350"
     CDS             2164..>2466
                     /gene="gloA"
                     /locus_tag="VN084_07350"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HWT29312.1"
                     /translation="MRMLHTMLRVGNLDRSIDFYTNILGMQVLRKDDYPDGKFSLAFI
                     GYGDEKDHTVIELTYNWGVESYDMGNAYGHIAIEVDDAYVACEKVKQAGGKVIREAG"
BASE COUNT          538 a          739 c          717 g          472 t
ORIGIN      
        1 accatcccat cgctcaaacg cgaggacctg cagcgcttct atacgactta ctatggcgca
       61 cgcaacgcag tgatcgccat gatcggtgat atggatcgtg cacaagccga acggctggcg
      121 gagcgtatca gcagcggctt gccgtcgtcg ccagcggtcg tgccgctgcc ctcggtaagc
      181 tatcccgaac atgcagtgga gcagcgtatc ccgcatccgg ccaagcaatc ccacatcctt
      241 ctcggttatc cgggcgtgaa acggggtgac ccggattact tcccgctgta tgtcggcaac
      301 tatgtgctgg gcggcggcgg tttcgtgtcg cgcctgactg aagaggtgcg cgaaaagcgc
      361 ggcctggtct atagcgtcta tagttatttc atgccgatgg ccgagctcgg accgttccag
      421 atcgggctgc agaccaagcg cgagcaggcg gaacaggcgc tcaaggtggt gcacgaggcc
      481 ctggacaagt tcatgaagga tggccccacc gaagccgaat tgaaggcagc caaacagaac
      541 atcaccggcg gattcccgtt gcggctcgac agcaacagca agatcctcga ttacctggcg
      601 gtcatcgggt tctacagatt gccgctgact tacctcgacg atttcaacaa gcaggtcaat
      661 gccgtcacca ccgcgcagat ccgcgacgca ttcaagcgcc gcctggatac caacaagctc
      721 gtgacgatca tcgtcggcgg cgacacggca ggcaacgaca gtaaggacaa tacggctaac
      781 taacccaggc ggcatttatt cgctacactg acgcccatgg cgagtcagaa gaataaagta
      841 cgcatcggtg gcggggaatg gcgcagccgg ctgctggcat tccccgatgt gcccggcttg
      901 cggccgacgc ctgaccgggt ccggcagacc ctgttcaact ggctggggca agacctgcat
      961 ggcctgaatt gcctggatct gttttgcggc accggtgtca tgggcttcga ggcgctgtcg
     1021 cgcggagcgc attcggtgac catggtcgaa aaggcgtcgc aggcctatcg ggccctgctc
     1081 gaaaatcagc gagcgctcgg cgcggaaaat gcccggatcg tccagatgga cgcgctacag
     1141 ttcctcgccg gcaatcaaca gatgttcgat gtcatctttc tcgacccccc ctaccagcaa
     1201 ggctggctcg acaaactgct gccagtcatc gcgccgcacc ttgcctctgg cgggatggtc
     1261 tatgccgagg ccgaattcgc cctcgcggat agtgacgatt ggcatatcca caaacagggc
     1321 agggcgggca atgttttctt ccacctgtta aaatcggccc atcacgattg agccactgtg
     1381 ccgagcgacc ccatgcggac caaagccatc taccccggaa ccttcgaccc tatcaccctc
     1441 ggccacgagg atgtggtgcg ccgtgccgcg cacctgttcg acgaagtggt ggtcgcagtg
     1501 gcgcagagcc acagcaaacg ccccttgttc agcctggatg aacgcgtggc gctggcggcc
     1561 gaagtgctgg cggattgtcc caacgtcaag gtcgtgggct ttaccggatt gctcatgcag
     1621 ttcgcccgcg aagaaggcgc gcgcgtggtg atccgtggcc tgcgtgccgt atcggatttc
     1681 gaatacgagt tccagctggc aggcatgaac cggcatcttt atcccgaagt ggaaacgttg
     1741 ttcctcaccc cggcagaaca gtacatgttc atctccgcca gcttggtgcg cgagatcgcg
     1801 cagctcggcg gcgatatcgg ccaattcgtc tccccgcgcg tacaagaacg tatcgctgac
     1861 aaacttgtag caaggtaatc ctgtatggca ttgatgatta ccgacgaatg catcaactgt
     1921 gacgtctgcg agccggaatg cccgaatggc gccatctcgc aaggcgaaga gatctacgtg
     1981 atcgacccgg acctatgcac cgaatgtgtc gggcatttcg atcgaccgca atgccaggag
     2041 gtctgcccga tcgactgtat cccgctcgac ccggctgtgg tggagagcaa ggaacaactg
     2101 caacagaaat acctgcgact catcgcagcc aaatagtcac atatacaatt caggagaaac
     2161 ttcatgcgaa tgctgcatac catgctgcgc gtcggcaacc tcgatcgttc catcgatttc
     2221 tataccaata tcctcggcat gcaagtgctg cgcaaggatg attatcctga cggcaagttc
     2281 tcactggcct tcatcgggta tggcgacgag aaggaccata ccgtcatcga gctcacctat
     2341 aactggggag tcgaaagcta cgacatgggc aacgcctacg gccacatcgc gatcgaggtc
     2401 gatgacgctt acgtcgcctg cgaaaaggtc aagcaggccg gtggcaaggt gatccgcgaa
     2461 gcaggc
//
LOCUS       DATMTW010000376         5054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_569292, whole genome shotgun sequence.
ACCESSION   DATMTW010000376 DATMTW010000000
VERSION     DATMTW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569292"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1507
                     /locus_tag="VN084_07355"
     CDS             242..1507
                     /locus_tag="VN084_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWT29313.1"
                     /translation="MSARLCIALTISVGIVSLASALPLHAAGYSLLEQGASGLGNAFA
                     GGAASAEDASTIYANPAGMTYLPDRQLVTTLHAVRVSSRFSDRGSIAAFGRPLGNEGG
                     DAGGFVAVPNIYYSQRIRPDLNIGIAINAPFGLKTDYDKQWIGRYQAVRSEIATLNIN
                     PAVAFKLNDRLSLGFGLSIMKMDAELTRVVNFGPAGDGFAQLKGDDWGYGYNFGTLWQ
                     ATADTRIGVAYRSRVRQHIEGDATFTRPPLIPSALVPNGNITTQLTLPESASISLFHQ
                     ASDKLALMADITWTGWHRLQDLKIQRSDGSLLVNIPENWQDTMRYSVGASYQLTPAWK
                     LRGGLAYDQESIKDRFRTVSIPGDDRTWLTIGAGYRLSERNQIDIAYAHLYVKDTSID
                     NDQGLVNARLRGDYHNKIDVLSLQYIHNF"
     gene            complement(1585..2604)
                     /gene="aroF"
                     /locus_tag="VN084_07360"
     CDS             complement(1585..2604)
                     /gene="aroF"
                     /locus_tag="VN084_07360"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWT29314.1"
                     /translation="MIIVMHADATEAQIEAVVQRIREHGLDINVSRGMERTLVGAIGD
                     ERKLSQELFETMPGVEQALHIVKPYKIVAREWHRLDSVIDIAGIPLGGKQIQIIAGPC
                     SVETPAQMDASALAVKAAGARLMRGGAFKPRTSPYTFQGAGIEGLEMLRTAARKHGLP
                     IVTELMDVRQLDTFIDYGVDVIQVGTRNMQNFDLLKALGKIDVPVILKRGMSATIAEW
                     LMAAEYIAAGGNHNIIFCERGIRTFETAYRNVLDVTSIPVLKRETHLPVIVDPSHAGG
                     KAWMVPALSRAAIAAGADGLLVEMHPKPCEAWCDADQAIDPVELHALMTELRGIAQVI
                     GREIA"
     gene            complement(2601..3719)
                     /gene="hisC"
                     /locus_tag="VN084_07365"
     CDS             complement(2601..3719)
                     /gene="hisC"
                     /locus_tag="VN084_07365"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWT29315.1"
                     /translation="MTNLCDLAPSYIRSIAPYQPGKPISDLAREMGLDPSQIVKLASN
                     ENPLGISPKARMAMEDAMEDIARYPDGNSFALRDAVRERFGVDSAQIVFGNGSNDILE
                     LAARAFLTAGTEAIYAQHAFAVYALVTQAVGARGVVVPAKDYGHDLEAMLAAITDKTR
                     MIFVANPNNPTGTLLDKTELLAFLRQVPPNVLVVLDEAYDEYLPLEIKSESIGWLGEF
                     PNLVISRTFSKAYGLAGLRIGFGLGAAEVIDIMNRVRQPFNVNSVAQAAAVASLQDEA
                     FVERSRALNQAGMLQITRGLEALGLEYIPSFGNFVSFRLANASGAYRQLLQRGVIVRP
                     IANYDMPDYLRVSIGLFDENTRFLDAMREIVAGAPGAS"
     gene            complement(3806..4969)
                     /locus_tag="VN084_07370"
     CDS             complement(3806..4969)
                     /locus_tag="VN084_07370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWT29316.1"
                     /translation="MLIGQNLLEHSSLHKWTLRFKTRRLELHYRLYALPRLRQQARIA
                     LVVGAVMYGLYGFLDVLFLPADAVLKVWYIRSGVILLALAALCFTFFKKYERYSQLCL
                     AVTGIVGGIGLMAKMSFLPDWALTYFYGGLILITFWVYCFCGLRFPNALLSGLALLAA
                     FNAAFLWIKTLPLIPMVSYNFFIVSANVVGAFACYTAEAQDRALFLREMELEKERRLQ
                     QDRALHDNLTSLPNRELLMDRVEQAINYAVRNDQICAGLYLDLDRFKPINDTYGHGVG
                     DQVLQEVARRFQRVLRDSDTLSRLSGDEFFVLARDIGSRPAAEVMAKKLLQQLEQPFE
                     IKQLPKIEHLSASIGICIFPYRDLSAVDVLRRADQAMYEVKRGSKAGYAFAAT"
BASE COUNT         1116 a         1528 c         1418 g          992 t
ORIGIN      
        1 aaaaccgaaa cgcatttcgg tagcagctgg cttggtccac atagtagctc tccttagcaa
       61 gtgattgaaa aatacagcga cccggtaact ccgtgatcag catgtgtagt gcattctccg
      121 catccagcct ggctcgcgac agtgcaaaaa tcacgaaact ctagtcatga ttatcatgag
      181 caggctttgc ccgcgcgggg tttggcacta gaatcgacaa aacaacaacg atgggccatc
      241 aatgtccgca agactttgta ttgcactcac catcagtgtc ggtatcgtca gcctggcgag
      301 cgcactaccc ttacacgccg caggctattc tctgcttgag caaggcgcca gcgggctagg
      361 gaatgctttt gccggcggcg cagccagtgc cgaggacgcc agcaccatat acgccaaccc
      421 ggccggcatg acctacttgc cggatcggca gctggtcacc acgctgcacg cggtccgcgt
      481 atcctcgcgt ttcagtgacc gcggatccat cgccgcgttc ggccgcccgc tagggaatga
      541 aggtggcgac gctggcggtt tcgtcgccgt gcccaacatc tattacagcc agcgcatccg
      601 gcccgatctc aatatcggca tcgccatcaa tgcccccttt ggtctcaaga ccgattacga
      661 caagcaatgg atcggccgct atcaggccgt aagatccgag atcgccacac tcaacatcaa
      721 cccagcggtc gcgttcaagc tcaacgatag actctccctt ggattcgggc tatcgatcat
      781 gaaaatggat gccgaactca cgcgcgtggt caattttggc ccggctggcg acggtttcgc
      841 ccagctcaag ggcgatgact ggggctacgg ttacaacttc ggcacgctat ggcaagccac
      901 ggctgacacc cgcatcgggg tcgcttaccg ctctcgcgtg cgccagcata tcgaaggcga
      961 cgcaacgttc actcggccgc cgctgatccc ctctgccctt gtgccgaatg gcaatatcac
     1021 gacacagctg actttgccgg aaagcgcctc gatcagcctg ttccatcagg ccagcgacaa
     1081 gttggcgctg atggccgata tcacatggac aggttggcat cgcctgcagg acctcaagat
     1141 ccagcgcagc gatggctcct tgctggtgaa catcccggag aactggcagg acacgatgcg
     1201 ctattccgtc ggcgccagct atcagctcac accagcctgg aagctgcgtg gcggacttgc
     1261 ctatgaccag gagtcgatca aggatcggtt ccgtacggtc agcatccccg gagacgaccg
     1321 aacctggctc acgatcggcg ccggttaccg gctcagcgag cgcaatcaga tcgacatcgc
     1381 ctacgcgcat ctctatgtca aggacacctc catcgataat gatcaaggcc tggtgaatgc
     1441 cagactgcgt ggggattacc acaacaagat cgatgtcctg agtctgcagt acatccataa
     1501 tttctgatcg acgggaaatc caacaaccgg caataaaaaa cgcgccaagg cgagctcttt
     1561 attgccgggt gcaaagggtc agcatcaagc gatctcgcgg ccgatcacct gcgcgatccc
     1621 tcgcaattct gtcatgagtg catgcagctc tacagggtcg atggcctgat cagcatcgca
     1681 ccaggcctcg catggcttgg ggtgcatctc caccagcagt ccgtcggcgc cggccgcgat
     1741 cgctgcacgc gataacgcgg gcaccatcca ggcttttccg cccgcatgcg aaggatcgac
     1801 aatcaccggc aggtgggtct cgcgcttgag tacagggatc gacgtcacat ccagcacgtt
     1861 gcgatatgcc gtctcgaacg tgcggatacc gcgctcgcaa aagatgatgt tgtgattgcc
     1921 gccagccgcg atatattccg cggccatgag ccattcagcg atcgtggctg acatcccgcg
     1981 cttcaggatc accggcacat cgatcttgcc cagggctttc aagagatcga aattctgcat
     2041 attgcgcgtg ccgacctgga tgacgtctac cccatagtcg ataaatgtat ccagttgtcg
     2101 cacatccatg agttcagtca cgatcggcag cccatgctta cgcgctgcgg tgcgcaacat
     2161 ctccaggcct tcgatgcccg ctccctggaa agtgtagggg ctggtgcggg gcttgaaggc
     2221 gccgccgcgc atcaatcgcg cccctgcggc cttgacggcc agcgccgaag catccatctg
     2281 tgctggcgtc tccaccgagc aaggcccggc gatgatctgg atctgcttgc cgcccagcgg
     2341 aatgcccgcg atatcgatga cactatccaa cctgtgccat tcgcgagcga cgatcttgta
     2401 aggcttgacg atatgcaggg cctgttcgac tccgggcatg gtctcgaaca gctcctggct
     2461 gagcttgcgc tcgtcaccga tggcgccgac cagggtccgt tccatgccgc gcgacacgtt
     2521 gatgtccagc ccgtgttcgc ggatacgctg aacgaccgcc tcgatctggg cctccgtcgc
     2581 gtcggcatgc atgacgatga tcatgaggcg cctggagctc ccgcaacgat ctctcgcatc
     2641 gcatcgagga agcgcgtgtt ttcgtcaaag aggccgatac tcacgcgcag gtagtccggc
     2701 atgtcatagt tggcgatcgg tcgcacgatg acgccacgct gcaacaactg gcgataggct
     2761 ccgctggcat tggccagacg gaagctgacg aaatttccga acgacgggat atattcaagc
     2821 cccagcgctt ccaacccgcg cgtgatctgc aacatgccgg cctgattcag cgcacgactc
     2881 cgctcgacga aggcttcatc ctgcagcgaa gcgacggcag cggcctgcgc cacgctattg
     2941 acgttgaacg gctgtcgcac gcgattcatg atgtcgatca cctctgccgc gccgagaccg
     3001 aatccgatac gcaggccggc gaggccatag gccttggaaa aggtgcggga gatgaccaga
     3061 ttggggaatt cgcccagcca gccgatggac tcggatttga tttcgagcgg caggtactca
     3121 tcgtaggctt cgtcgagcac caccagcaca ttaggcggca cttgtcgcag gaacgccagc
     3181 aattccgtct tgtccagcaa ggtgccggtc ggattgttgg gattggcgac gaagatcatg
     3241 cgcgtcttgt cagtgatcgc agccagcatg gcttcaaggt cgtggccata gtccttggcc
     3301 ggcaccacca ctcctctggc gccaaccgcc tgcgtcacca gcgcataaac ggcaaaggca
     3361 tgctgggcgt agatcgcctc ggtccctgct gtcagaaagg cccgtgccgc cagctccagg
     3421 atatcgttgg agccgtttcc gaacacgatc tgcgcgctat cgacaccgaa tctctcacgc
     3481 acggcgtcac gtagcgcaaa gctgttgccg tccggatatc gtgcgatatc ctccatcgca
     3541 tcctccattg ccatgcgggc tttgggactg atgccgagtg ggttctcgtt ggaggcgagc
     3601 ttgacgatct ggctcggatc cagtcccatc tcgcgcgcta ggtcagagat cggtttgccc
     3661 ggctggtaag gcgcgatcga gcggatatag gatggggcca ggtcacacaa attggtcacg
     3721 gttcgaacct cggatggaat tgggtcacaa ataaaaatgc ggcttacgcc gcgtggtgtc
     3781 aaagatattt tagcatcagc gcctgtcagg tggcggcaaa tgcatagccg gccttgctac
     3841 cacgcttgac ctcgtacatc gcctggtcgg cgcgacgcag cacatcaacg gcactgagat
     3901 cacggtaagg gaagatacag atgccgatgc tcgctgaaag gtgttcgatc tttggcaact
     3961 gcttgatctc gaacggctgc tccagctgtt gtaacagctt cttggccatc acctctgccg
     4021 cagggcggct gccgatatcc ctcgccagta cgaagaactc gtctccgctc aggcgtgaaa
     4081 gcgtatcgga atcgcgcaac acgcgctgaa aacgccgggc cacttcctgc agcacctgat
     4141 cgccgacccc atggccatag gtgtcgttga taggcttgaa gcgatcaagg tcaagataca
     4201 ggccggcaca gatctggtcg ttgcgtacgg cgtagttgat ggcttgctca acacgatcca
     4261 tcaacagctc gcgattaggt agcgaagtca gattgtcatg caatgcgcga tcctgctgca
     4321 ggcggcgctc cttctccagc tccatctcac gcaggaacag cgcccggtcc tgtgcctcgg
     4381 ctgtgtaaca cgcaaatgca ccgaccacgt tggccgagac gatgaaaaag ttataactca
     4441 ccatgggaat caacggcaac gtcttgatcc agaggaacgc agcattgaaa gccgccagca
     4501 acgccaaccc actcagcaac gcatttggga aacgcaggcc acaaaaacaa tagacccaga
     4561 aagtgatgag gatcagcccg ccatagaaat aagtcagtgc ccaatcgggc aaaaacgaca
     4621 tcttggccat gagaccgatg ccgccgacga tgccggtcac cgcgaggcaa agctggctgt
     4681 agcgttcgta tttcttgaag aaagtgaaac agagtgcggc gagggctagc aggatgacgc
     4741 cgctacgaat ataccagacc ttgagcaccg catctgccgg caggaacagg acatcgagaa
     4801 agccatacag gccatacatg acagccccga ccaccagcgc gatacgcgcc tgctggcgaa
     4861 gtcttggcaa ggcatagaga cggtaatgca gctcaagacg ccgggtttta aagcgcaacg
     4921 tccatttgtg cagggaggag tgttcgagta ggttttgacc gatcagcatg aatcgctaac
     4981 gacatccctg aatcgcttgt tgtactgcta tctaagcacc tttgtcagat cactgcaact
     5041 gggtaggagc ctaa
//
LOCUS       DATMTW010000377         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_164937, whole genome shotgun sequence.
ACCESSION   DATMTW010000377 DATMTW010000000
VERSION     DATMTW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164937"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..352
                     /locus_tag="VN084_07375"
     CDS             137..352
                     /locus_tag="VN084_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29317.1"
                     /translation="MKAMLLGGSACVVLGIALGLLQLWFAPWSPEVFVKVELTLAALF
                     AIALVIWFTRSEYREYKKQRNDPRLDD"
     gene            466..711
                     /locus_tag="VN084_07380"
     CDS             466..711
                     /locus_tag="VN084_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29318.1"
                     /translation="MNVLSSTLTVCWDAEQESLLILHPDRMQFEFPLIEIRDSTLNSM
                     DWPTACQFIGERLVLLIPALREKYIDTETGRLRGVEP"
     gene            714..1076
                     /locus_tag="VN084_07385"
     CDS             714..1076
                     /locus_tag="VN084_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29319.1"
                     /translation="MALIRPRARWLTAWLCMLLAGYCAAIILIWIHAGVSPADLLADT
                     SPLYASGIYLLVCILVGLGVASVLLSRQEISQPARLAILAGLALTSLPSAWLMQLVVP
                     LWLLPLWYAWSFYRDADA"
     gene            1125..1307
                     /locus_tag="VN084_07390"
     CDS             1125..1307
                     /locus_tag="VN084_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29320.1"
                     /translation="MSALVWYFGFAALLAFYALPLPSLASQAEVAKKHPEFADALYRT
                     SRALSGIAAARSRREC"
BASE COUNT          260 a          507 c          417 g          317 t
ORIGIN      
        1 agctcatgtg cgaacaggcg cggcgcaaca aggccaccgc cgtcatcgcg gtcaagtacg
       61 acgccaccga agtcatgcgc ggcgtcaccg aagtgctctg ctacggcacc gccgtgatcg
      121 tgcggccgaa gcaggcatga aagccatgct cctcggcggc agcgcctgcg tcgtcctcgg
      181 catcgcgctc ggcctgctcc agctctggtt cgccccgtgg agccccgaag tcttcgtcaa
      241 ggtcgaactc acgctggccg ccctcttcgc catcgccctg gtgatctggt tcacgcgcag
      301 cgaataccgc gaatacaaga agcaacgcaa cgacccgcgc ctcgacgact gatagcgaat
      361 tgccaccggt aatcgcaagg gcgatgccga cgccacacgc acctccggcg gcgcgctcct
      421 tgctctccgc cccgccccgc ccccctttgc cgaaccggag ccgccatgaa cgttctttct
      481 tccacgctca ccgtctgctg ggatgcggag caggaatcgc tgctgattct gcacccggat
      541 cggatgcaat tcgagtttcc cctgatcgag attcgcgact ccacgctgaa ttcgatggat
      601 tggccgaccg catgccagtt catcggcgag cgactggtct tgctgatccc ggcgctgaga
      661 gaaaaatata tcgacaccga aacggggaga ctacgcggag tcgaaccttg agcatggcct
      721 tgatacggcc tcgtgcgcgc tggctaacgg cctggctgtg catgctgctt gccggctact
      781 gtgcagcgat tatcctgatc tggattcatg ccggagtttc gcccgccgat cttcttgccg
      841 ataccagtcc gctgtatgcg agcggcatct atctgcttgt ctgcatcctg gtcggactcg
      901 gagtcgccag cgtactcctg tcgcggcaag agattagcca gcctgcacgc cttgccatcc
      961 tggcaggact cgccttgact tcgctgccct ctgcctggct tatgcaactg gtcgtccccc
     1021 tctggctgtt gccgctctgg tatgcatgga gcttctatcg cgatgccgat gcctgatagc
     1081 gcattcctgt acgaagccgc acccaatctt tcgctggagc ctgtatgagc gcgctggtct
     1141 ggtacttcgg ttttgcggcg ctgctggcat tctatgcgtt gcccctgccc tcgctggcaa
     1201 gccaggccga agtcgcaaag aagcacccgg aattcgccga cgcgctttac cgcacaagca
     1261 gagcgctttc tggcatcgcg gctgcccgat caagacgcga gtgctgatat tccatgcacc
     1321 ggccgaagcc gatgaggcca tccggctgat gcgcttctgc gcctttgtcg gctatttttt
     1381 cctgctggca accatactca catgggcgtt cgatggataa agcctctctc cgcccttcct
     1441 gaatccgagg ttacatacac atgccacccg tagcgcgcac gccgctctcg cttcacgaga
     1501 c
//
LOCUS       DATMTW010000378         1866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_368492, whole genome shotgun sequence.
ACCESSION   DATMTW010000378 DATMTW010000000
VERSION     DATMTW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368492"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VN084_07395"
     CDS             complement(<1..483)
                     /locus_tag="VN084_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWT29321.1"
                     /translation="MTNLKQAVGQWLANLTRRLRHHFYLYLAALLTLLLLLDAGVFGI
                     GERISQRTFDAMLHSRILVPPADTDILIVDVNEASLSAMAGEYGQWPWPRQVFGEFLE
                     NIEAQRPKAVVFDILFSEADIDNPDSDAYFNETIGDTSNTYFPFLRLPAEQDKLSEVK
                     P"
     gene            603..>1866
                     /gene="rmuC"
                     /locus_tag="VN084_07400"
     CDS             603..>1866
                     /gene="rmuC"
                     /locus_tag="VN084_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWT29322.1"
                     /translation="MIETLLLILVTLCLLVLLWLAWRQANAADSTTVLLHQLEDKHRA
                     MLLDLHNGLNTLGDRLQESAVNNSERLRAAVAEELKQTRDAMLALQSTQTASLAQTRE
                     NILERLHTTLAEQGKAEQELIQATMRHATLQLTTSMEALTKTVDGRLEQIGGKVSERL
                     EEGFKKTNETFTNVMTRLATIDEAQKKIDGLTTNVVSLQELLGDKRSRGAFGEVQLEG
                     LVRNLLPETAFEFQYTFANNTRADCVLKLPEPTGLVAVDSKFPLESYEAMFLDGAERT
                     GTGVFKTAVRKHIDDIAGKYIIPNVTSDGAVMFIPAEAVFAEIHAHHRDLVEHAQKKR
                     VWIVSPTTLMAVLNTARAVLKDTETRKQVHIIQDELGKLGKDFARFDERMKKLATHIQ
                     QASKDVDEVHVSSRKITERFAKIERVELE"
BASE COUNT          409 a          549 c          574 g          334 t
ORIGIN      
        1 gggcttcacc tcactcagct tgtcctgttc ggcaggcagg cgcaggaagg ggaaataagt
       61 attgctggtg tcgccgatgg tctcgttgaa ataggcgtca ctatcgggat tgtcgatatc
      121 cgcctcgctg aacaggatat cgaatacaac ggcttttggc cgttgtgcct cgatgttctc
      181 caggaattcg ccgaacacct ggcgcggcca gggccattga ccgtattcgc cggccatggc
      241 gctgaggctc gcttcgttga catcgacgat gaggatgtcg gtatcggccg gaggcaccag
      301 gatgcgacta tgcagcatgg cgtcgaaagt gcgctggctg atgcgctcgc ctatcccgaa
      361 tacgccggca tcgagcagca gcaatagcgt gagcagagcg gcaagataca gatagaagtg
      421 atggcgcagg cgtcgggtca ggttcgccag ccactggccg accgcttgtt tgagattggt
      481 cacggcagcg cttgccccag tgaaaagatg cccgagtttg gcataaaccc cgacagattt
      541 gaagtttacg cgcacgggcc gctcgaggtg gatgcgccgt gtcatggagt ataattccgc
      601 cgatgatcga gaccctcttg ctgatcctag tcacactttg cctgctggtg ctgctctggc
      661 tcgcctggcg ccaggcgaac gctgccgatt ccactacggt gctgctgcat caactggaag
      721 acaagcatcg cgccatgctg ctcgatctgc acaacggcct caatacgctc ggcgaccgat
      781 tgcaggaaag cgccgtcaat aacagcgagc gcctgcgtgc cgcagtcgcc gaagagctga
      841 aacagacccg cgacgccatg ctcgcgctac agtcgacgca aaccgccagc ctggcgcaga
      901 cgcgcgagaa catcctggag cgcctgcata ccacgctggc cgagcagggc aaggccgagc
      961 aggagctgat ccaggccacg atgcgccacg ccacgctgca attgacgacg agcatggagg
     1021 ccctgaccaa gacggtggac ggccgcctgg agcagatcgg cggcaaggtc tcggagcgac
     1081 tggaagaagg cttcaagaag actaacgaga cctttaccaa tgtcatgacg cggcttgcca
     1141 ccatcgacga ggcgcagaag aagatcgatg gcctcaccac caacgtcgtc agcctgcagg
     1201 agttgctggg cgacaagcgt tcgcgcggcg ctttcggtga agtgcaactt gaaggcctgg
     1261 tgcgcaatct gctgccggaa acggcattcg agttccagta caccttcgcc aacaacacgc
     1321 gcgccgactg cgtgctcaag ctgcccgagc cgactggcct ggtggcggtg gattccaagt
     1381 ttccgctgga aagctatgag gccatgttcc ttgatggcgc tgagcgtacc ggcactggtg
     1441 tattcaagac cgccgtgcgt aagcatatcg atgatatcgc aggcaagtac atcattccca
     1501 acgtgacgtc agacggcgcg gtcatgttca tcccggccga agccgtgttc gccgagatcc
     1561 acgcgcatca ccgcgatctc gtagagcatg cgcagaagaa gcgtgtctgg atcgtctcgc
     1621 cgaccacgct gatggcggtg ctcaacaccg cgcgtgccgt gctcaaggac accgagacgc
     1681 gcaagcaggt gcatatcatc caggacgaac tgggcaagct gggcaaggat ttcgcgcggt
     1741 tcgacgaacg catgaagaag ctcgccactc acatccagca ggcgagcaag gatgtcgacg
     1801 aagtccatgt cagcagccgc aagatcaccg agcgcttcgc caagatcgag cgtgtggaac
     1861 tggaag
//
LOCUS       DATMTW010000379         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_636396, whole genome shotgun sequence.
ACCESSION   DATMTW010000379 DATMTW010000000
VERSION     DATMTW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636396"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VN084_07405"
     CDS             complement(<1..772)
                     /locus_tag="VN084_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWT29323.1"
                     /translation="MTTDTHTPQAPAESLAKSFDPKDIEGRWYQFWEGRGYYAAGADA
                     SKQDNFCILLPPPNVTGTLHMGHGFNQTIMDALTRYHRMRGANTLWQPGTDHAGIATQ
                     IVVERQLDAQGVSRHDLGREKFLEKVWEWKAYSGGTITQQMRRLGTSPDWSRERFTMD
                     AGLNKVVTESFVRLYNEGLIYRGKRLVNWDVKLGTAVSDLEVVQEEEDGHLWHIRYPL
                     SDGSGHLTVATTRPETMLGDVAVMVHPEDERYRKLIGQT"
     gene            complement(785..1699)
                     /locus_tag="VN084_07410"
     CDS             complement(785..1699)
                     /locus_tag="VN084_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWT29324.1"
                     /translation="MLRKWCSRYLNAITLSLHAGLLLAALIAFDEAGAEVWLLFLFAS
                     LFARLYAWHFNLRRLLAIVEHPTSTIAAAAQGYVELRGRARQLLPTRSPLTQTPCVWF
                     RYWVYVRDQNNVWRLADYLSSEQHFELEDLTGRCVVDPANAEVMLAERHARTQHDHKY
                     IELLIREGKPLYVLGELETVTAQTAARQLSRETSELLADWKKAPERLKKRFDLNGDGE
                     IDLQEWAQAREVAAQEVLTRHGLADRQEQHKIRAPRDGRLFLISAVSPEQLRHRYRFW
                     VSLHLALAALAAVLVLLISYRHALGVLW"
     gene            complement(1703..2269)
                     /locus_tag="VN084_07415"
     CDS             complement(1703..2269)
                     /locus_tag="VN084_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWT29325.1"
                     /translation="MVVIGLVVLGVVLVYVIMVYNSLVTIRNNVDKAWANIDVLLKQR
                     HDELPKLVDTCKQYMKHEQETLEKVIQARARVSEAQAAHDVAALGVAEGALRAGLGSL
                     FALAEAYPELKANQNFAHLQTRITGLENAIADRREFYNDSVYINNVRVQQFPEMVVAR
                     LFGFVEQQMLKFAAAELKDVDIARRFNA"
     gene            complement(2363..2860)
                     /locus_tag="VN084_07420"
     CDS             complement(2363..2860)
                     /locus_tag="VN084_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWT29326.1"
                     /translation="MTTALQSPVTTLLDTNAIAYEVIEIPLSEDKKPIRNLEALLSNA
                     GLDPRSVVRSVLFRAESGQFVLLAAAGGGRADWALLRNHLNERKVRMAEFDEVPEATG
                     YVVGAVPPIALPDSVRVLVDAGVKAYDKVVIGSGVLGYALALGSADLLALMADAETGA
                     FLKAE"
     gene            complement(2917..>3943)
                     /locus_tag="VN084_07425"
     CDS             complement(2917..>3943)
                     /locus_tag="VN084_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HWT29327.1"
                     /translation="YLRHGCLTLREAFDHVLEKTDGQAQKLLFEFAWRDYWRQVWYRQ
                     GEAILSDMEPAKVLRGTQPLPQDVLDGDTGLPCMDAFIHDLQTQGYVHNHARMWFAAW
                     LLHWRKVDWRAAADWYEAELLDGDVASNHLSWQWVASTFSAKPYIFNKENLARYSGER
                     YCRDCRCACPFDASYEQLQAQLFSAPAPMVARRYDNHPPAPVRLSPSGISAVLFHDEM
                     LSPAHALLQSGDLKLFVFDPSLQGAWSRKRLQFVADCLIEMEAVEVWQGGLADVLHHL
                     GVAEVVTQRTPNPDLQALLHDQRVTWHAEPAFAPVSLGENALKRFSRYWQKVGPALLG
                     DRHYRKP"
BASE COUNT          726 a         1240 c         1237 g          740 t
ORIGIN      
        1 cggtctgacc gatcagcttg cgatagcgtt cgtcttccgg atgcaccatc acggcgacgt
       61 cgcccagcat ggtctctggc cgcgtggtag cgaccgtcag gtgccccgaa ccatcactta
      121 gcggatagcg gatatgccac aagtggccgt cttcttcctc ctgcaccact tcgaggtcgg
      181 acacggcggt accgagtttg acgtcccagt tcaccaggcg cttgccgcga tagatcaggc
      241 cttcgttgta gagccgtacg aagctctcgg taaccacctt gttgaggccg gcatccatgg
      301 tgaagcgctc gcgcgaccag tcaggcgagg tgccgaggcg acgcatctgc tgggtgatgg
      361 tgccgccgga ataggctttc cactcccaga ccttttccag gaacttctcg cggccaaggt
      421 catgccgcga cacgccttgc gcatcgagct gacgctcgac gacgatctgc gtggcgatgc
      481 cggcatggtc ggtgcccggc tgccacaggg tattggcgcc gcgcatgcgg tgatagcgcg
      541 tcaacgcgtc catgatggtc tggttgaacc cgtgccccat gtgcagcgtg ccggtgacgt
      601 ttggcggcgg cagcaggatg cagaaattgt cctgtttcga ggcatcggcg ccggcggcat
      661 agtaaccgcg gccctcccag aactggtacc agcggccttc tatgtccttg ggatcaaacg
      721 attttgccag cgattcggcc ggcgcttgcg gggtatgggt gtcggtagtc ataagcgcgc
      781 gattttacca cagcaccccc aacgcatgcc ggtagctgat gagcagcacc agcacggcgg
      841 cgagcgccgc cagcgccaga tgcaagctca cccagaaccg atagcgatga cgcagctgct
      901 cgggcgaaac ggccgagatg aggaacaggc gaccatcgcg cggcgcgcgg atcttgtgct
      961 gctcctgccg atcggcaagg ccatggcgcg tcagcacctc ctgcgccgcc acttcgcgcg
     1021 cctgcgccca ttcctgcagg tcgatctcgc cgtcgccatt gagatcgaac cgcttcttca
     1081 atcgctccgg ggccttcttc cagtccgcca gcagctcgct ggtctcccga ctgagctggc
     1141 gcgccgccgt ttgcgcggtg acggtctcca gctcacccag tacgtacagg ggcttgccct
     1201 cacggatcag tagctcgatg tacttgtggt catgctgggt cctggcatgg cgctcggcca
     1261 gcatgacttc ggcgttggcc ggatcgacca cgcagcgacc ggtcaggtct tccagttcga
     1321 aatgctgctc gctcgataga tagtccgcca gccgccagac attgttctga tccctgacgt
     1381 agacccagta gcggaaccag acgcatgggg tttgcgtgag cggactgcgc gtaggcagca
     1441 attgccgcgc acggcccctg agttcgacat agccctgcgc cgcagcggcg atcgtggaag
     1501 tgggatgttc gacgatggcg agcaggcggc gcaggttgaa atgccaggca tagagcctcg
     1561 cgaacaggct ggcgaacagg aacagcagcc agacctcggc cccggcctca tcgaacgcga
     1621 tcagcgcggc cagcagcagg ccggcatgca ggctcagggt gatcgcgttg aggtagcgac
     1681 tgcaccactt gcgcagcatc gctcaggcgt tgaagcgtct ggcgatatcg acgtccttga
     1741 gttcggcggc ggcgaacttg agcatctgtt gctcgacgaa accgaacagc cttgccacga
     1801 ccatctccgg gaattgctgc acgcgcacgt tgttgatata gacgctatcg ttgtagaact
     1861 cgcggcgatc ggcgatggcg ttctcgagac cggtgatgcg ggtctgcaag tgggcgaaat
     1921 tctggttggc cttgagctcc gggtaagcct cagccagtgc gaacaggcta ccgagcccgg
     1981 cgcgcaacgc accttccgcc acgcccaagg ccgcaacatc atgcgcggcc tgtgcctcgg
     2041 agacccgggc gcgcgcctgg atcacctttt ccagggtctc ctgctcatgc ttcatgtatt
     2101 gcttgcaggt atccaccagc ttcggcagct cgtcgtgacg ctgcttgagc agcacgtcga
     2161 tattggccca ggccttgtcg acgttgttgc ggatggtcac gaggctgtta tagaccatga
     2221 tgacgtagac gagcacgacc ccgagcacga ccagaccgat gactaccatg attgctgcct
     2281 cccctgttgt ttcgcagagt gtaatctgca gcggcgagcg aaacaatcat gcggtgccgc
     2341 ctcagtcggc atgttcaggt catcactccg ccttgaggaa ggcgccggtc tcggcatcgg
     2401 ccatgagtgc cagcaggtcc gccgatccca gggccagcgc gtagccgagg acgccgctac
     2461 cgatgacgac cttgtcatat gctttcacgc ctgcgtccac cagcacccgc acactgtccg
     2521 gcaaggcgat cggcggcacg gcgccgacca catagccggt cgcttccggc acctcgtcga
     2581 attcagccat gcggactttg cgttcgttga gatggttgcg cagcaaggcc caatcggcac
     2641 gaccgccgcc tgcggcggca agcagcacga actggccgga ttcggcgcgg aacaatacgc
     2701 tacggaccac tgatctgggg tccaggcctg cattcgagag caacgcttcc agattccgga
     2761 tcggcttctt gtcctcgctc aacgggatct cgatcacctc ataggcgata gcgtttgtgt
     2821 ccagcagtgt ggtgacaggc gattgcaggg ctgtggtcat gtgggcttcc cgaggataag
     2881 tgacgatggc gtcattgtgg catagcccag cccgcgtcag ggcttgcggt aatgccgatc
     2941 gccgagcaag gccgggccga ccttttgcca gtagcgtgaa aaccgcttga gcgcattctc
     3001 gcccagactc acaggggcaa aggcgggctc ggcgtgccag gtcacgcgct gatcatgtaa
     3061 cagcgcctgc agatctgggt tgggcgtgcg ctgggtgacg acttcggcca cgcccaagtg
     3121 atgcagcaca tcggcgaggc cgccctgcca gacttcgacc gcttccatct cgatgaggca
     3181 atcggcgacg aactgcagcc gctttcgcga ccaggcgcct tgcagagatg ggtcgaagac
     3241 gaacagtttg agatcgcctg actgcaacag cgcatgtgcg ggcgacagca tctcgtcatg
     3301 gaacagcacc gccgagatgc cgctgggtga aagtcgcaca ggtgcaggcg gatggttgtc
     3361 gtagcgcctc gccaccattg gtgccggcgc actgaagagc tgcgcctgca actgctcgta
     3421 gctcgcgtcg aacgggcacg cgcagcgaca gtcccggcaa tagcgctcgc cgctatagcg
     3481 cgcaagattc tccttgttga agatataggg cttggcagaa aacgtggacg ccacccattg
     3541 ccaggaaaga tggttcgagg cgacgtctcc atccagcagc tcggcctcgt accaatccgc
     3601 cgcggcacgc caatccacct tgcgccaatg cagcagccag gcggcgaacc acatgcgcgc
     3661 gtggttgtgc acgtagccct gcgtttgcag atcgtgaatg aacgcatcca tgcatggcaa
     3721 gccggtgtcg ccgtcgagca cgtcctgcgg aaggggttgg gtgccgcgca agaccttggc
     3781 gggttccatg tcgctgagga tggcctcacc ctgccggtac cagacctgtc gccagtagtc
     3841 gcgccaggcg aattcgaaca gcagcttctg cgcctggccg tcggtcttct ccagcacatg
     3901 gtcgaaggct tcgcgcaggg tcaggcagcc atgccgcagg taa
//
LOCUS       DATMTW010000380         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_535881, whole genome shotgun sequence.
ACCESSION   DATMTW010000380 DATMTW010000000
VERSION     DATMTW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535881"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VN084_07430"
     CDS             complement(<1..410)
                     /locus_tag="VN084_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWT29328.1"
                     /translation="MTTAKNNDKPAMRLLTGAFILSCLVSCTQPKDAQKVDYDKAYPV
                     ESGGSLVEATRGEPSGLIPMIAGESAASAVAANIFNTLLKYDKNLDIEGDLAQSWEVS
                     QDQKTITFKLKPGMKWADGQPLTSADALFTWKLVT"
     gene            complement(427..1797)
                     /locus_tag="VN084_07435"
     CDS             complement(427..1797)
                     /locus_tag="VN084_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWT29329.1"
                     /translation="MPELDSEYTRNHESWYAARPDYVKRMVARSQRYLYHIVEEVEKR
                     GMPTEIALLPMVESAFNPQAYTRSKAAGIWQFIPSTGKDFGLKQNWWVDNRRDVTAAT
                     DAALNYLQRLHVMFGTWDLALAAYNAGEGTVRRAIERNRKQGLPTDYESLPLPPETRN
                     YVPKLQAVKNIMTHPEQYGLEILPIPNRPYFTKVNAPEQIDAHLAAKLAEISYEEFSS
                     LNPEYNRPVITASGKVHEILLPVGTAETFKSNLEGYDKPLVSWQTYHAKRGERMDSIA
                     RKFGISLAQLRDINDLPTRKKIAMSRQILVPNAKASADTGNLVAVATDMSDTPAASEA
                     GASEAGGSNLHRVAKGDSLAMIAKRYGTTSRALMQRNHLKSSKLKIGMTLAIAPASGK
                     ITQAQRAEKPLRTAHRYVVRKGDTLSGIARKFDVGLEDLQRWNNIDGTQINPGNRLYV
                     VNPGEA"
BASE COUNT          312 a          538 c          622 g          451 t
ORIGIN      
        1 gtgaccagtt tccaggtgaa gagcgcgtcg gcgctggtta agggctggcc atcggcccat
       61 ttcataccgg gtttaagctt gaaggtgatg gtcttctggt cttgcgatac ttcccaggat
      121 tgcgccaggt cgccttcgat gtcgaggttc ttgtcgtact tgagcaaggt attgaagata
      181 ttggctgcga cggcagaggc ggctgactcg cccgcgatca tggggatcag gccgcttggt
      241 tcgccacggg tcgcctccac cagggaaccg ccactctcga ccggataggc cttgtcgtag
      301 tcgaccttct gcgcatcctt cggctgggtg cagctgacga gacacgacaa aataaatgcg
      361 cctgtcagaa ggcgcatggc gggcttgtca ttgtttttgg ccgttgtcat cgcatcctag
      421 agacaactag gcttcgcccg ggttcactac atacagtctg ttcccgggat tgatctgggt
      481 accatcgata ttgttccagc gttgcaggtc ctcgagcccg acgtcgaact tgcgtgcgat
      541 gccgctcagg gtgtcgcctt tgcgcacgac atagcgatgg gcggtgcgta acggtttttc
      601 cgcgcgctgc gcctgggtga tcttgccgct tgccggagcg atggcaagtg tcatgccgat
      661 cttgagtttg ctgcttttca ggtgattgcg ttgcatcagt gctcgcgagg tggtcccgta
      721 gcgtttggcg atcatggcca ggctatcgcc cttggcgacg cgatgcagat tcgagccgcc
      781 agcctcgctg gcacctgcct cgctggcggc aggggtgtcg gacatgtcgg tagcgacggc
      841 caccagattg ccggtatcag ccgacgcttt ggcgttcggc accaggatct ggcgggacat
      901 ggcgatcttc ttgcgcgtgg gcagatcgtt gatgtcgcgc agctgggcca ggctgatgcc
      961 gaacttgcgg gcgatactgt ccatgcgttc gccacgcttg gcatggtagg tctgccagct
     1021 gaccagcggt ttgtcatagc cttccagatt ggatttgaag gtctctgccg tgccgaccgg
     1081 cagcaggatc tcgtgcactt tgccacttgc ggtgatcacc gggcggttgt attccggatt
     1141 gagcgaggag aactcttcgt aactgatctc cgccagcttg gccgccagat gcgcgtcgat
     1201 ctgctccggc gcgtttacct tggtgaaata ggggcgattg gggatcggca ggatctcgag
     1261 gccatattgc tcgggatggg tcatgatgtt cttcaccgcc tgcagtttgg ggacgtaatt
     1321 gcgcgtctcc ggcggcaggg gcaggctctc gtaatctgtc ggcaggccct gcttgcgatt
     1381 gcgctcgatg gcgcggcgca cagtgccttc gccggcgtta taggcggcaa gtgccagatc
     1441 ccaggtgccg aacatgacat gcaggcgctg caggtagttg agcgcggcat cggtggccgc
     1501 cgtgacgtca cggcgattat ccacccacca gttctgcttc agtccgaagt ccttgcctgt
     1561 cgagggaatg aattgccaga taccggcggc tttgctgcgc gtatacgcct gcgggttgaa
     1621 agcgctctcg accatgggca gcaacgcgat ctcggtcggc atgccgcgct tttccacttc
     1681 ttccacgatg tgatagagat agcgctggct acgcgcaacc atgcgtttca catagtctgg
     1741 ccgggccgca taccaggatt catggttgcg cgtgtattcg gaatcgagtt ccggcatgcc
     1801 gtacccctcg cggatacgca gccacaggtc gtcttccggc gatagctcgg ctggattttc
     1861 cagcgcggcg atctcgctgt cgtcaggcag gtaactgggg acgggggtcg atcggggtca
     1921 ggc
//
LOCUS       DATMTW010000381         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_469014, whole genome shotgun sequence.
ACCESSION   DATMTW010000381 DATMTW010000000
VERSION     DATMTW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469014"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VN084_07440"
     CDS             complement(<1..797)
                     /locus_tag="VN084_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWT29330.1"
                     /translation="MSLLLVASLGMFVGLLMGLTGAGGGIVAVPLLMVFLPVTFANAV
                     PVAMLATAMAAGVGTILGLRSGLVRYRAAGLMAAVGILIAPFGIWLSHRLDPMILTVL
                     FAGVLLLVACRTICNRNPELPHAADETLPCLVDRNTGRLIWNSRCSSSLMLSGGLAGL
                     LSGMIGVGGGFVIVPALQRLSVLSMHSVLHTSLAVIFLVSTSVVIGYAGLGAIDWQLA
                     IPFSMTAVFGLALGRRFVSRWSPRVLRLAFAVLAVGVAVMLLVRAFA"
     gene            complement(794..1213)
                     /locus_tag="VN084_07445"
     CDS             complement(794..1213)
                     /locus_tag="VN084_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HWT29331.1"
                     /translation="MKLLVSFVSGLLFGLGLILSGMTDPRNIVDFLDVSGAWNPRLLF
                     VMAGAVFVSFFAFRYAGRRSRSMLDEPMQIPTNRKLDMRLVVGATLFGIGWGLAGYCP
                     GPALASVATGLMQPLVFVLAMLVGMACFEVLEQRRKP"
     gene            complement(1213..1647)
                     /locus_tag="VN084_07450"
     CDS             complement(1213..1647)
                     /locus_tag="VN084_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HWT29332.1"
                     /translation="MNGLDILAIQQSLGGGLLIGLAVSLLIVLNGRIAGISGILGGLL
                     NAETVDVAWRVAFLAGLVLAPMVYGHFGRLPVVEIDANTTMLVIAGLLVGLGTRYGAG
                     CTSGHGICGVARLSPRSLVATAVFMSSAMLTVYIARHVIGAV"
     gene            complement(1644..>1828)
                     /locus_tag="VN084_07455"
     CDS             complement(1644..>1828)
                     /locus_tag="VN084_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWT29333.1"
                     /translation="RVGLRQPSLSQQLTVLRNEALVATRREGKQVYYRIASEDALAIM
                     QVLYRKFCVDKSEVGK"
BASE COUNT          441 a          615 c          521 g          251 t
ORIGIN      
        1 caggcgaacg cccgcaccag caacatcacg gccacgccga cggccaatac ggcaaaggcg
       61 agcctgagca ctctcggtga ccatctgctg acgaaccggc gacccaaggc aagcccaaaa
      121 acggcggtca tcgagaacgg gatcgccagt tgccaatcga tcgcacccaa gcccgcataa
      181 ccgatcacga ccgatgtgct gaccaggaag atgacggcca gcgacgtatg caggacactg
      241 tgcatggaca gtacggagag ccgctgcagt gcaggcacga tgacgaatcc gccgcccacg
      301 ccgatcatgc cggagagcag gccggccaat ccgccagaca acatcagcga gctcgaacag
      361 cggctgttcc agatcaggcg cccggtattg cggtcgacta ggcaaggcaa ggtttcgtcc
      421 gcagcgtgag gcagttccgg gttacggttg cagatggtac ggcaagccac cagtaacaag
      481 acgccagcga acagcacggt caggatcatc ggatccaatc ggtgcgatag ccagatgccg
      541 aatggcgcga tcaggatgcc gacggccgcc atcaagccag cggcacgata gcgcaccaag
      601 cccgagcgca gccccagaat ggtaccgaca cccgccgcca tcgcggtggc gagcatcgcc
      661 accggcaccg catttgcgaa agtcacaggc aggaatacca tcaacaacgg tacggcaacg
      721 atgccgccgc cggcgccggt tagccccatc aataagccaa cgaacatgcc tagcgatgcc
      781 accagcaaca ggctcatggc ttgcgtcttt gctccagcac ttcaaagcag gccataccga
      841 ccaacatcgc cagcacaaag accagcggct gcatcaggcc tgtcgccact gaggccagtg
      901 caggccccgg gcaatacccg gccaggcccc agccgatgcc gaacagcgtc gcgccaacca
      961 ccaatcgcat atccagcttg cggttggtcg ggatctgcat gggctcgtcc agcatcgaac
     1021 ggctgcgccg gccggcatag cggaacgcga agaagctgac gaacacggcg cctgccatca
     1081 cgaacaacag cctgggattc catgcgccgc tgacatcgag gaaatcgaca atgttgcgcg
     1141 ggtccgtcat gccggagagg atcagcccga ggccgaacaa cagcccgctg acaaagctca
     1201 cgagtaattt catcacacag ctccaatcac atggcgggcg atatagacgg tcagcatggc
     1261 actcgacatg aacactgccg tcgccaccag cgacctcggc gagaggcgcg ccacaccgca
     1321 gatgccatga ccactggtgc aacccgcgcc gtagcgggta ccaagcccga ccagcagacc
     1381 ggcaatcacc agcatcgtgg tgttggcatc gatctcgacg accggcaaac ggccgaagtg
     1441 gccatacacc atcggcgcca gcaccagacc ggcaaggaag gcgacgcgcc aggcgacgtc
     1501 tacggtctct gcattcaaaa gcccgccgag gatgccactg atcccggcga tacgcccgtt
     1561 gagcacgatc agcaagctga ccgcgagtcc gatcagcaag ccgccgccga gcgattgttg
     1621 tatcgccaag atatccagac cgttcattta cccacctccg atttatctac gcagaatttt
     1681 cggtacaaca cctgcatgat cgccagcgcg tcttcgctgg cgatccggta atagacctgc
     1741 ttgccttccc tgcgggtggc caccagcgcc tcattgcgca agacggtcag ctgctgcgag
     1801 aggctcggct gacgcaagcc gacacgcg
//
LOCUS       DATMTW010000382         4781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_569907, whole genome shotgun sequence.
ACCESSION   DATMTW010000382 DATMTW010000000
VERSION     DATMTW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569907"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VN084_07460"
     CDS             complement(<1..486)
                     /locus_tag="VN084_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWT29334.1"
                     /translation="MTQKLSCLIVDDETDIRELIALTLERMGIIAETASNLDDARHLL
                     SKRDFALCLTDMRLPDGNGLDLVRYISQHHPGLPVAVITAYGSADNAVSALKAGAFDY
                     LSKPISLKQLRPLVESALKLHAPGMPASGGLKLIGESAAITQARAMIEKLARSQAPVY
                     IS"
     gene            complement(483..2093)
                     /locus_tag="VN084_07465"
     CDS             complement(483..2093)
                     /locus_tag="VN084_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWT29335.1"
                     /translation="MTDAVTRDDQGVAKTYWRSLGYLGYYRITIALLFLGAYLFLGRD
                     IWWNNYNSQLYFALSSSYLFFGLFASGLTFVQWPRFNHLLTLQISIDIVFIVSLMYSV
                     GGIKSGVGLLLVVAIAAASLISQGRLALFYAALASIALLLEQSYRMLTVQGDVNDYSH
                     AVMLSLSCFATAWLAHSLAKRSDQSEALASQRGIDLENLAQVNELITQEMQDGVVVID
                     RELRLRHHNLQAEGLLKPLQPNWEAMQVDHCLPELARLMRQWLEDGLDASTVRLQAEQ
                     RELILRFMPVGHTRDQGAVIFIEDWSQIQAQAQQLKLAALGKLTANIAHEIRNPLSSI
                     SHASQLLQEDEGRDPASARMLQIIDDNVQRMDLMVKDILELNRRDRTRQEVIEVQAFL
                     LEFHAEFCQVEKIPRHGFLLELAGEALAIHFDRRHLNQILWNLCRNGWRHSQQVDGSL
                     RLHLRLSQLSHGVLLEIADDGPGVAADMRQHLFEPFFTTESTGTGLGLYIARELCEAN
                     AASIHYTGHDFGGSFTIHIKQSVSETLP"
     gene            complement(2090..2317)
                     /locus_tag="VN084_07470"
     CDS             complement(2090..2317)
                     /locus_tag="VN084_07470"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HWT29336.1"
                     /translation="MAKILLLAIAIWLLYTVVKRYLGSVASSEQPPAAEDIVPCAHCG
                     VHLPRSDSIAVDQRHYCGEACREAYRREHQV"
     gene            2467..3057
                     /locus_tag="VN084_07475"
     CDS             2467..3057
                     /locus_tag="VN084_07475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWT29337.1"
                     /translation="MATAKELSDFLAQVERRAFKQTVYAVRDDHAALDIVQDAMLKLA
                     EKYAAKPAAEYPMLFQRILQNTMRDYWRRQKVRNLWTSLLSSFGAGNDDDDERDPLET
                     LEVDDEAAQPMEQLERKQTIAIIEQALQRLPTRQREAFVLRYWEEMDVAETAAVMGCS
                     EGSVKTHCSRAVHALALILEKEGLSKQGIGLSGDAR"
     gene            3054..3434
                     /locus_tag="VN084_07480"
     CDS             3054..3434
                     /locus_tag="VN084_07480"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HWT29338.1"
                     /translation="MRQERDTAKDIVTLLDASTQQLDTQIVSRPEQARHAAVARAQAT
                     GPMLAGHAGLLQGFLDYLAHHRMVMPAAMMCGAVMLAFVVTQQFVGQDGYEQGDAFLL
                     ASDLPPEAYLDKGFDEWLERTSEF"
     gene            3407..3853
                     /locus_tag="VN084_07485"
     CDS             3407..3853
                     /locus_tag="VN084_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HWT29339.1"
                     /translation="MVRAYVGVLILLSGLLSGVAVAQDEVLKPTWTQLSDEQRQVLAP
                     LSAEWDTLRPWQRERMLDIARDYPKMKPEQQERVQKRLNKWSRMTPFERENARKRYQE
                     FKALSPEKKEAFRKKWQEYRQLPAEERAKWRKQNPDMYGPDDPDSE"
     gene            3910..4338
                     /locus_tag="VN084_07490"
     CDS             3910..4338
                     /locus_tag="VN084_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWT29340.1"
                     /translation="MQLSHNSGLLRRLASMVYDGLLLVALLFVVTFLFTLVFGSVLQR
                     QERYVLQALLWLVAGIYFIAYWTHGGQTLAMQTWRIKLVDHRGALLTPGKAALRYLAA
                     TAGLLFFGAGLVWAVFDHEGLFLHDRLVGTRIVRSVDNPH"
     gene            complement(4396..>4781)
                     /locus_tag="VN084_07495"
     CDS             complement(4396..>4781)
                     /locus_tag="VN084_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWT29341.1"
                     /translation="PELLNVLLVVPEKMSAWNLYFYIHHLSANKQKTSRHEIALWSKM
                     IYPLACLVMVVLALPFGFLHQRSGGASAKIFAGIMLGIVYQVMNRVFVHLGLLNDWSA
                     LLSAVVPTLLFLVAGVCMLFWVERR"
BASE COUNT          988 a         1367 c         1503 g          923 t
ORIGIN      
        1 gctgatatag accggtgcct ggctgcgggc gagcttctcg atcatggcgc gcgcctgtgt
       61 gatcgccgcc gattcgccga tcaacttgag gccgcctgat gcgggcatgc cgggcgcatg
      121 cagtttaagg gccgattcca ccagcggccg cagctgtttc aaggagatcg gtttgctcag
      181 gtagtcgaat gcccctgcct tgagtgcgga gacggcattg tcggcactgc cgtaggctgt
      241 gatcaccgcg accggcaatc ccggatgatg ctggctgatg tagcggacga gatcgagccc
      301 attgccgtcg ggtagtcgca tgtcggtgag gcagagcgcg aaatcgcgct tgctaagcag
      361 atggcgcgcg tcatccaggt tacttgcggt ttcggcgatg atacccatgc gttcgagcgt
      421 gagggcgatc agctcgcgga tatcggtttc atcatcgacg atcaggcagg agagtttctg
      481 ggtcatggca aggtctcgga cacggactgc ttgatgtgga tagtaaagga accaccgaaa
      541 tcatggccgg tgtaatggat gctggcggca ttggcttcgc agagttcacg tgcgatgtag
      601 aggccaaggc cggtgcccgt gctttcggtg gtgaagaaag gttcgaacag gtgctggcgc
      661 atgtcggcgg cgacaccggg tccatcgtcg gcgatctcca gcaagacgcc gtgacttagc
      721 tgtgatagtc gcaggtgcag tcgcaggctg ccgtcgacct gctggctgtg gcgccagccg
      781 ttgcggcaca ggttccacag gatctggttg agatgcctgc gatcgaaatg gatggccagg
      841 gcttcgcctg cgagttcgag caggaagcca tgtcgcggga tcttttccac ctggcagaac
      901 tcggcatgga attccagcag gaaggcctgc acctcgatca cctcctggcg ggtacggtcg
      961 cgacgattga gttcgaggat atccttcacc atgaggtcca tgcgctggac gttatcgtcg
     1021 atgatctgca gcatgcgcgc ggatgccggg tcgcgccctt catcctcctg cagtagctgg
     1081 ctggcatggc tgatcgagct taatgggttg cggatctcgt gagcgatgtt ggccgtgagc
     1141 ttgcctagcg cggccagttt gagctgctgg gcctgcgcct ggatctggct ccagtcctcg
     1201 atgaagatca ctgcgccctg atcgcgggta tggccgactg gcatgaatcg caggatgagt
     1261 tcgcgttgct cggcctgcag cctgacggtg ctggcgtcca ggccatcttc cagccactgc
     1321 cgcatgagtc tggccagttc gggcaggcaa tggtccacct gcatggcttc ccagttcggc
     1381 tgcaggggct tgagcaagcc ttcggcctgc aagttgtggt ggcgcaggcg aagttcgcgg
     1441 tcgatgacga ctacgccgtc ctgcatctcc tgggtgatga gctcgttgac ctgcgccagg
     1501 ttctcgaggt cgatgccgcg ctgcgatgcg agcgcttcac tctggtcaga gcgcttagcc
     1561 agactgtgcg ccagccaagc ggtggcgaaa cagctcaggc tgagcatgac cgcatgtgag
     1621 taatcgttga cgtcgccctg cacggtcagc atgcggtacg actgttccag tagtagcgcg
     1681 atgctggcga gcgcagcata gaacagggcg agccgccctt ggctgatgag gctggcggcg
     1741 gcaatcgcca ccaccagcag caggccgacc ccgctcttga tgccgccgac ggagtacatc
     1801 aggctgacga tgaaaacgat atcgatgctg atctgcaagg tcagcaggtg attgaaccgg
     1861 ggccactgga cgaaggtcag accggaagcg aagagtccga agaagagata gctggaggat
     1921 agtgcgaaat agagctggct attgtagttg ttccaccaga tatcgcgccc aaggaagagg
     1981 taggcgccca ggaacagcag cgcgatggtg atccggtagt agccgaggta accgagcgag
     2041 cgccagtaag tcttggcgac cccctgatca tcgcgcgtca ccgcgtcggt catacctgat
     2101 gctcgcggcg gtaggcttcc cggcatgcct cgccgcaata gtgccgctga tcgacggcga
     2161 tgctgtcgct gcgcggcagg tgcacgccgc aatgcgcgca aggtacgata tcctccgcgg
     2221 caggcggctg ctccgaagac gcgacgctac caagataacg tttcacgacc gtatagagta
     2281 accagatggc aatcgccaat aatagaatct tcgccatatc gtctgccagc agtggagtcg
     2341 gggagagtcc gtcgattgta agcgaaaagc caatccgggg acaaaaatca aacctgctat
     2401 actctccgcg gaacagcccg tgctcacggg gcttcaactc atggcagcag gtgtgacaga
     2461 cacaggatgg caacagcaaa ggaactatcg gattttctcg cgcaagtaga gcgtcgtgcg
     2521 ttcaagcaga cggtctacgc agtgcgtgac gatcacgctg cgctggatat cgtgcaggat
     2581 gccatgctca aactggccga aaagtacgca gcgaagccgg ccgccgaata tcccatgctt
     2641 ttccagcgta tcttgcagaa cacgatgcgc gattactggc gcaggcagaa ggtgcgcaat
     2701 ctgtggacca gcctgctgtc ctcgttcggt gccggtaacg acgatgacga cgaacgcgat
     2761 cccctcgaga ccctggaagt cgatgacgaa gccgcgcagc ccatggagca gcttgagcgc
     2821 aagcagacca ttgctataat cgagcaagcg ctgcaaagat taccgacgcg tcaacgtgag
     2881 gccttcgtct tgcgttactg ggaggaaatg gatgtcgccg agaccgccgc cgtgatggga
     2941 tgttccgagg gcagcgtgaa gacgcattgc tcgcgagccg tgcatgcatt ggcgctcatc
     3001 ctcgaaaaag aaggattaag caagcagggg atcggactat caggagacgc gcgatgagac
     3061 aggaacgaga caccgccaaa gacatcgtca cattgctgga cgccagcacg caacagctcg
     3121 acacacagat cgtgtcgcgc ccggaacagg cacgccatgc ggcagtcgcc cgtgcgcagg
     3181 cgaccggccc catgctggcc ggccacgcgg ggcttttgca agggttcctc gattatctcg
     3241 cgcaccatcg tatggtcatg cctgcggcca tgatgtgcgg cgccgtgatg ctggcattcg
     3301 tggtgacgca gcaatttgtc ggtcaggacg gctacgagca gggcgacgcc ttcctgctcg
     3361 cttccgacct gccgcccgag gcatatttgg ataaagggtt tgatgaatgg ttagagcgta
     3421 cgtcggagtt ctgatactgc tctccggatt actttccgga gtcgcggtcg cccaggacga
     3481 ggttttgaaa cccacctgga cccaactcag cgacgaacaa cgccaagtgc tggctccgct
     3541 atcagccgaa tgggatacgc ttcgaccatg gcagcgcgaa cggatgctgg atatcgcccg
     3601 ggattaccca aagatgaagc ccgagcagca agaacgggtg caaaagcggc tgaacaaatg
     3661 gagccgcatg acgcctttcg agcgtgagaa cgcgcgcaag cgttatcagg agttcaaggc
     3721 actctcgccg gaaaagaaag aagccttccg caaaaaatgg caggaatatc gccagttgcc
     3781 agcagaggaa cgcgccaaat ggcgcaagca gaatccggac atgtatggcc cggatgaccc
     3841 ggactcggaa taagcgcaga cgttagcgcg gtcgatcgcc ttatcgtccg ttccctttca
     3901 tcactccaag tgcaactctc ccacaattcc ggtctgctca ggcggctcgc cagcatggtc
     3961 tatgatggcc tgttgctggt cgcgctgctg ttcgtcgtga cattcctctt cacactggta
     4021 ttcggtagtg tcctgcaacg ccaggagcgt tacgtcctgc aggcgctgct ctggctggtg
     4081 gccggcatct atttcatcgc ttattggacc cacggcggcc agaccctcgc catgcagacc
     4141 tggcgcatca aactcgtgga tcaccgcggg gcgctgctca cgccgggcaa ggcggcgcta
     4201 cgttaccttg ccgccactgc cggcttgctg ttcttcggcg caggcctagt ctgggccgtg
     4261 ttcgatcacg aaggcttgtt cctgcatgac cggttggtcg gcacgcgcat cgtccggagc
     4321 gtcgacaacc ctcattaaga atacgctttt agcgaacgcc tgcaggcacc tctcagcggc
     4381 tggtcctgcc agacgtcagc gtcgttctac ccagaacagc atgcacaccc ccgccaccag
     4441 gaacagcagt gtggggacca ccgcgctcag tagcgcagac cagtcgttaa gtagtcccag
     4501 gtgcacgaag acgcgattca tgacctggta gacgatcccc agcatgatgc cggcgaatat
     4561 cttggcgctg gcgccgccgg agcgctgatg caggaagccg aacggcagcg cgagcacgac
     4621 catcaccagg caggcgaggg gatagatcat cttcgaccag agcgcgatct cgtgacgcga
     4681 ggtcttttgc ttgttggcgc tcaagtgatg gatgtagaag tacaggttcc acgcggacat
     4741 cttctcaggg acgaccagca gcacattgag caactccggt c
//
LOCUS       DATMTW010000383         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_200710, whole genome shotgun sequence.
ACCESSION   DATMTW010000383 DATMTW010000000
VERSION     DATMTW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200710"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VN084_07500"
     CDS             <1..480
                     /locus_tag="VN084_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HWT29342.1"
                     /translation="HTQKLLHLDIKPANIYIRIDGSPVLLDFGSARQTLSETESKLPP
                     SYTPGFASPEQYYDRSLLGPWSDIYSVGATMYACLVRAAPVAADQRLKKDTLVPATKL
                     GKEVYSNNLLELIDRCMALDHMQRPQSMFALQKALLEDMPLQDKKTGLMDKIKTYLK"
     gene            517..1440
                     /locus_tag="VN084_07505"
     CDS             517..1440
                     /locus_tag="VN084_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWT29343.1"
                     /translation="MKFTIHQGSRQGPRPYNQDRLAYSYSKDALLIVLADGMGGHRHG
                     EVAAQLAVKTLTDAFQEEAQPKLKNPAGFLKHAIQYVHDAIGALTIAHDMLEAPRTTI
                     VAAILQDDHLYCAHVGDSRLYHFREGRLKFKTEDHSLVQVLYNRGQLDKDDMLTHPQR
                     HKIYNCLGGDKQPQIALTQTDDLFEGDTLLLCSDGLWSVLDDTEIADMLHSGLVTETV
                     AALLDEAERRSQVEGDNMSAIGLQWGDPHHHLFAVSTVTMPLSATTTIMNPLTHQPGQ
                     GEAAAPADLTDDEIEHAIAEIQAALKKSARN"
     gene            1507..2223
                     /gene="rph"
                     /locus_tag="VN084_07510"
     CDS             1507..2223
                     /gene="rph"
                     /locus_tag="VN084_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWT29344.1"
                     /translation="MRPSHRAPNELRTVEIIRHYTKHAEGSVLVKFGDTHVLCTASVE
                     EKVPGFLKGKNQGWVTAEYGMLPRSTGSRMDREAARGKQSGRTQEIQRLIGRSLRAII
                     NLEKLGERSIQIDCDVIQADGGTRTASITGAYVALHDAVTLLLKKGLITESPLIDAVA
                     AVSVGVYQGQPVLDLDYIEDSDCDTDMNVVMTGNGGFVEIQGTAEGEPFDRKAMDAML
                     DLAAAGIRELVAQQRSVLEG"
     gene            2225..2824
                     /gene="rdgB"
                     /locus_tag="VN084_07515"
     CDS             2225..2824
                     /gene="rdgB"
                     /locus_tag="VN084_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWT29345.1"
                     /translation="MLPFDKLVIATGNAGKLREIRQLLAPLAVEVLPQSDFAVPEAEE
                     PYCTFIENALAKARHASLHTGLPALADDSGICVDALQGAPGVYSARYAGEPRSDARNN
                     EKLLTAMTGHSNRHAHYYCVMVLVRHAEDPQPLIAEGLWQGEIMTAPRGDGGFGYDPV
                     FLDAKTEKTGAELPLEIKNRISHRGQALTRMLHMLERLQ"
     gene            2821..3039
                     /locus_tag="VN084_07520"
     CDS             2821..3039
                     /locus_tag="VN084_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29346.1"
                     /translation="MSDNQWPKWRRDDQSVVSCTEKVKVMKENFDELKQMAQDALEDG
                     LLMEVSEAQMREALHQLVDKLVNPYNKK"
     gene            3036..>3179
                     /locus_tag="VN084_07525"
     CDS             3036..>3179
                     /locus_tag="VN084_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880827.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HWT29347.1"
                     /translation="MIPIIPADAAPSSGQATLSGLSTPPPLALYIHIPWCVRKCPYCD
                     FNSH"
BASE COUNT          694 a         1024 c          927 g          534 t
ORIGIN      
        1 cacacgcaga aactgctgca cctcgatatc aagccggcca atatttatat tcgcatcgac
       61 ggttcacccg tgttgctgga tttcggttcg gcacgccaga ccctgagcga gaccgagagc
      121 aagctgccac cgagctacac gcccggcttc gcctcgcccg aacagtatta cgaccgcagc
      181 ctgctcggcc cctggagcga catctacagt gtaggcgcca ccatgtacgc ctgcctggtc
      241 agggcggcgc cagtggcggc ggatcaacgc ctgaaaaaag acacgctggt gccggcgacc
      301 aagctcggta aggaggtcta ctcgaacaac ctgctggaac tgatcgaccg ttgcatggcg
      361 ctcgaccata tgcagcgccc gcagagcatg ttcgccctgc agaaagcgct gctggaggat
      421 atgccgctgc aggacaagaa gaccgggctc atggacaaga tcaagaccta tctcaaatag
      481 gctgatcgat gaacggcaac gcatgagaac cagcgcatga aattcaccat ccatcagggc
      541 agccgccagg gcccacgccc ctacaaccag gaccgtctgg cctattccta cagcaaggac
      601 gccttactca tcgtgctcgc cgacggcatg ggcggccatc ggcacggcga agtcgcggcc
      661 cagctcgcgg tgaagacgct gaccgacgcc ttccaggaag aggcgcagcc caagctgaag
      721 aatcccgccg gcttcctcaa gcatgccatc cagtatgtgc atgacgccat cggcgcactg
      781 accatcgccc atgacatgct ggaagcgccg agaaccacca tcgtcgccgc catcctgcag
      841 gatgatcacc tgtattgcgc gcatgtcggt gattcacgcc tgtatcattt ccgcgagggt
      901 cggctcaagt tcaagaccga ggaccactcg ctggttcagg tcctctataa ccgcggtcag
      961 ctcgacaagg acgacatgct gacgcatccc cagcgacaca agatctacaa ctgcctgggc
     1021 ggcgacaagc aaccccagat cgcattgacg cagaccgacg atctgttcga aggcgatacc
     1081 ttgctactgt gcagcgatgg gctctggtcg gtgctggacg ataccgagat cgccgacatg
     1141 ttgcatagcg gcctcgtcac cgagactgtg gctgcactgc tcgacgaggc cgagcgacgt
     1201 agccaggtcg agggcgacaa catgagcgcg atcggtttgc agtggggcga tccgcaccac
     1261 cacctgttcg cggtctctac cgtgaccatg ccgctgagcg cgacgaccac catcatgaac
     1321 ccgctcaccc accagccggg ccagggcgaa gcggcggccc ccgccgacct gacagacgac
     1381 gagatcgaac acgccatcgc cgagatccag gctgcgctga agaagtctgc gcgcaactga
     1441 gcctggctgt cagggtcgaa tcggcaatgg cttataatgc cctcttcgaa cattcaggaa
     1501 attgccatgc gtcccagtca ccgcgccccc aacgaactcc gcaccgttga gatcatccgt
     1561 cattacacca agcatgccga gggctcggtc ctggtgaagt tcggcgatac ccatgtgctg
     1621 tgcacggcca gcgtcgaaga gaaggtcccg gggttcctca agggcaagaa ccagggctgg
     1681 gtcaccgctg aatacggcat gttgccgcgc tcgaccggca gccgcatgga tcgcgaagcc
     1741 gcccgtggca agcaatccgg ccgcacacaa gagatccagc gcctgatcgg gcgcagcctg
     1801 cgtgccatca tcaacctgga aaagctaggc gaacgcagca tccagatcga ctgtgacgtc
     1861 atccaggcag atggcggcac gcgcacggcc agcatcaccg gcgcttatgt cgcgctgcat
     1921 gatgcggtca cgctgctgct gaagaaaggc ctgatcacgg aatcgccgct gatcgacgcc
     1981 gtcgccgcag tctcggtcgg cgtctatcag ggccagcctg tgctcgatct cgattacatc
     2041 gaggattccg actgcgatac cgacatgaac gtggtcatga ccggcaacgg cggttttgtc
     2101 gagatccagg gtacggcaga gggcgagccg ttcgaccgca aggccatgga cgcgatgctc
     2161 gacctcgccg ccgccggtat ccgcgagctc gtcgcccaac aacgatcggt gctggagggc
     2221 tgaagtgctg ccattcgaca agctcgtcat cgccaccggt aacgccggca agttgcgcga
     2281 gatccggcaa ctgctggcgc cactggcggt cgaggtgctg ccgcagtcgg attttgccgt
     2341 gcccgaggcc gaagagccct attgcacctt catcgagaac gcgctggcca aggcccgcca
     2401 cgcgagcctg cataccggcc tgccagccct ggccgacgac tccggcatct gtgtagacgc
     2461 cctgcagggc gcgcccggag tctactccgc acgctatgca ggggaaccgc gctccgatgc
     2521 gcgcaacaac gaaaagctgc tgacggccat gaccgggcac tcgaatcggc atgcgcacta
     2581 ttattgcgtg atggtactgg tacgccacgc cgaggacccg cagccgctga tcgccgaagg
     2641 cctctggcaa ggcgagatca tgaccgcccc gcgcggcgac ggcggtttcg gttatgaccc
     2701 ggtattcctc gatgccaaga ccgaaaagac cggcgccgag ctgccgctgg agatcaagaa
     2761 ccgtatcagc caccgtggtc aggcgctgac caggatgctg catatgctgg aaaggctgca
     2821 atgagcgata accaatggcc taaatggcga cgcgacgacc agtcggtggt ctcatgcacc
     2881 gaaaaggtca aggtcatgaa agagaacttc gatgagctga aacagatggc ccaggatgcc
     2941 ctggaagatg gcttgctcat ggaagtctcc gaagcgcaga tgcgcgaagc cttgcatcag
     3001 ctggtggata agctggtcaa tccctacaac aaaaaatgat ccccatcatt ccggccgatg
     3061 ctgcgccttc atccgggcag gcaacacttt ccgggctatc gaccccaccg ccgctcgcac
     3121 tctatatcca tatcccctgg tgcgtgcgca aatgcccgta ttgtgatttc aattcacat
//
LOCUS       DATMTW010000384         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_435583, whole genome shotgun sequence.
ACCESSION   DATMTW010000384 DATMTW010000000
VERSION     DATMTW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435583"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VN084_07530"
     CDS             <1..635
                     /locus_tag="VN084_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HWT29348.1"
                     /translation="LCPHGFARVMPWDLMESYTLENGATRLILQIMHTPVAQKQLSYP
                     YNLSLTIDIGATLKIALTTTNRGNQPFVIGEAFHTYFQVSDVGNIAVTGLEGCEYADK
                     VLNYDRDTQQDAIHFDGEFDRVYVNTKADCVIEDAGLNRRIRIAKTGSNATVIWTPWR
                     EKAHGLGDMGLDESWRNMICVESANAMENIVMVSPNQSHVLAVEYSTESM"
     gene            730..>1921
                     /locus_tag="VN084_07535"
     CDS             730..>1921
                     /locus_tag="VN084_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HWT29349.1"
                     /translation="MKRITPILMLCLAMGFFAMAVQPAQAASLGRLTVISDLDQPLIA
                     EVELLGVNVTQLGEYSAQLASEQAYRIQGIEKTPPQYSIRVEVVRKFDDSPVLKLTSG
                     QPLHSRSTNILLQLDWPNGRIMREYALLLEPSQVANSAPVPVDSVVFTKNRVAPVADV
                     PPASSQPTSQTAAQPVQMPGNDPGERASSTDTSKDSRRPDKLGSERPSAEPKSVSPDS
                     VAAKSGYTTNAGDTLSRIAGRMQVDGVSLDQMLRALYLANKDAFIDGDINKLRVGAEL
                     KSPSAADFAAATAPAVTAHSEDWRSYRNQVAQMTADSPSNETGEGELPSGGKLAAVED
                     KAQVAKPGTHDVLKLSRSERIDEKWLSREAQFEQEKQALEEEVTVRENRLEDANQRIA
                     ALEKQ"
BASE COUNT          422 a          610 c          553 g          336 t
ORIGIN      
        1 cgctctgccc gcatggtttc gctcgcgtca tgccgtggga tctcatggag tcctacacgc
       61 tggaaaacgg cgccacgcga ctgatcctgc agatcatgca tacgccggtc gcgcagaagc
      121 agttgtccta tccctacaac ctgtcactca ccatcgacat cggcgccacc ctcaagatcg
      181 cgctgactac gaccaatcgc ggcaaccagc ccttcgtcat cggcgaggcc ttccatactt
      241 acttccaggt cagcgatgtc ggcaatatcg ccgtcaccgg tcttgagggc tgtgaatatg
      301 ccgacaaggt gctgaattac gaccgtgata cgcagcaaga cgccatccac ttcgatggcg
      361 agtttgaccg tgtctacgtc aataccaaag ccgattgcgt catcgaggac gctggcctca
      421 accgccgtat ccgtatcgcc aagaccggca gcaacgcgac cgtgatctgg acgccctggc
      481 gtgaaaaagc ccatggcctt ggcgacatgg ggctggatga gagctggcgc aacatgatct
      541 gcgtcgaatc ggcgaatgcc atggaaaaca tcgtcatggt gagccccaac cagtcgcatg
      601 tcctggccgt ggagtacagc accgaatcca tgtaaggcaa gcggccggca gttagcgcat
      661 gcctgcgatc cgatccatac tcccccatgc agttgcacgc atgaggttga ctgcgcgata
      721 gcgagagcca tgaaacgcat aacccctatt ctgatgctct gcctcgccat gggctttttc
      781 gccatggccg ttcaacctgc ccaggcggcc agtctcggca ggttgaccgt gatttccgat
      841 cttgaccagc cactgatcgc cgaagttgaa ctgctcggcg tcaacgtgac gcaacttggt
      901 gaatattcgg cccagctcgc ttccgagcag gcctatcgga ttcagggcat cgagaagacg
      961 ccgccgcaat acagcatccg cgtcgaagtg gtccgcaagt tcgacgacag ccccgtactc
     1021 aagctgactt ccggccagcc cttgcacagc cgttccacca atatcctgct tcagctcgac
     1081 tggcccaacg gacgcatcat gcgtgaatat gccctgctgc ttgaaccgag tcaggtcgcc
     1141 aatagcgctc cggtgccggt cgacagcgtg gtcttcacca agaatcgcgt agcgccagtg
     1201 gcggatgtac cgcctgcgtc atcccagcca acgagccaga cggccgccca gccagtccag
     1261 atgccgggca acgatccagg cgaacgggct tcgtcgaccg atacgtccaa agacagtcgt
     1321 cgcccggaca agctggggag cgagcggcca tccgctgaac cgaagtcagt cagtcctgac
     1381 agcgtcgcgg ccaagtccgg ctacaccacg aacgccggcg ataccttgag caggatcgcc
     1441 ggccgcatgc aggtcgatgg cgtcagcctc gatcagatgc tgcgcgcgct ttacctggcg
     1501 aacaaggatg ccttcatcga cggcgatatc aacaagctga gagtcggtgc cgagctgaaa
     1561 tccccttcgg ccgccgattt cgccgctgcg accgcccccg ctgtcacggc gcattctgaa
     1621 gactggcgca gttaccgcaa tcaggtcgcc cagatgacgg ccgacagccc atcgaatgag
     1681 accggtgaag gcgagttgcc cagtggcggc aagctggccg cggtcgagga caaggcgcaa
     1741 gtggccaaac cgggaacgca cgatgtgctc aagctttcgc gcagcgaacg catcgacgag
     1801 aaatggctga gccgcgaagc gcaattcgag caggagaaac aggcgttgga ggaagaggtc
     1861 acggtgcgcg agaatcgcct ggaagatgcc aaccagcgta tcgcagcgct ggaaaagcaa
     1921 g
//
LOCUS       DATMTW010000385         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_502288, whole genome shotgun sequence.
ACCESSION   DATMTW010000385 DATMTW010000000
VERSION     DATMTW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502288"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VN084_07540"
     CDS             <1..1030
                     /locus_tag="VN084_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWT29350.1"
                     /translation="VVDVLNGIQGATDVAAIRISGNAELDIRLDRAKLARYGMSVNDV
                     NTTVQTALAGSAANTFYDGDRRFDVTLRLDRTYRDAVDDIAELPVALPDQAGTIPLSA
                     IADIEIKQGASRVSREGVSRIVSIKANLIGRDQGSFVKEAMAKVKQKVKLPPGYTMTW
                     GGQFENQQRAMKRLEVIVPLSLMLIFILLFTAFRSMRNALLVLLMIPFTLIGGLAGLG
                     LAGLHLSISAAVGFIALAGISVQNGVIMVEQIKHLIREGKSLADSVLEGAVARLRPII
                     MTALMAGLGLLPAALSHGIGSETQRPFAVVIVGGLVTATFFTLLLLPLLFPYFTDEAR
                     TPKEPATS"
     gene            complement(1058..1540)
                     /gene="ispF"
                     /locus_tag="VN084_07545"
     CDS             complement(1058..1540)
                     /gene="ispF"
                     /locus_tag="VN084_07545"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWT29351.1"
                     /translation="MIRIGQGFDVHQLVEGRRCVIGGVEIPHENGLDGHSDADVLLHA
                     ICDALLGAAALGDIGKHFPNTDERYKDIDSRQLLTHVAKLLEAKHYSIVNIDATVIAE
                     APKLAPYIDQMRANIAADCQILLDTVNVKATTTEKLGFTGRGEGIAAEAICLIQCYRL
                     "
     gene            complement(1537..2250)
                     /gene="ispD"
                     /locus_tag="VN084_07550"
     CDS             complement(1537..2250)
                     /gene="ispD"
                     /locus_tag="VN084_07550"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWT29352.1"
                     /translation="MASFHVIIPAAGSGSRMGSELPKQYLPLLGHPLIAHTLSVFSVS
                     PRIASVHVVLSEDDVDWQRLMPVLDAKIKIWRCGGETRAATVLNGLEAIAGQLGDEDW
                     VLVHDAARPGLNQALLDRLLDSLQEDAVGGLLAIPLADTLKRADAESRVAKTESRENL
                     WQAQTPQMFRYGLLRQALQAAGGAPTDEAQAVEAQGFRPKLVPGELRNLKVTYPQDLA
                     MAEAILTADNQRQASRRTT"
BASE COUNT          459 a          770 c          719 g          476 t
ORIGIN      
        1 agtggtcgac gtgctgaacg gcatccaggg cgcgaccgat gtcgctgcca tccgcatctc
       61 gggcaacgcc gaactcgata tccgtctgga tcgcgccaag ctggcgcgct acggcatgag
      121 cgtcaacgac gtcaacacca ccgtgcaaac ggccctggcc ggcagtgccg ccaatacctt
      181 ctatgacggc gaccgccgct tcgacgtcac cctgcgcctc gaccgcacct atcgcgatgc
      241 ggtcgacgat atcgccgaac tgccggtcgc tttgccggac caggccggta ccatcccgct
      301 cagtgccatc gccgacatcg agatcaaaca gggcgcctca cgcgtcagcc gtgaaggtgt
      361 ctcgcgtatc gtctccatca aggccaatct gattgggcgc gaccagggca gtttcgtcaa
      421 ggaagccatg gccaaagtga aacagaaggt caagctgcca ccgggctaca ccatgacctg
      481 gggcggtcag ttcgaaaacc agcaacgcgc catgaagcgg ctggaagtca tcgtgccgct
      541 ctcgctgatg ctgatcttca tcctgctgtt cacggctttc cgctccatgc gtaacgccct
      601 gctggtgctg ctgatgatcc cgttcacgct gatcggcggg ctcgcgggac ttgggcttgc
      661 cgggctgcac ctgtcgatct cggccgcagt cggctttatc gccctggcgg ggatatcggt
      721 acagaacggg gtcatcatgg tagagcagat caaacatctg atccgtgagg gtaaatcact
      781 ggcggattcg gtgctggaag gggcggtggc acggctacgc cccatcatca tgacggcatt
      841 gatggccggc ctgggcttgc tgccggcggc gttgtcgcac ggcatcggct cggagacgca
      901 acggcccttt gccgtggtga tcgtcggcgg cctcgtgacc gcgacgttct tcacgctgct
      961 gctgctgccg ctgctgttcc cctacttcac cgacgaggcc cgcacgccca aggagcctgc
     1021 gacaagctag cttagccgcg atatcgaggt cgctcgacta gagccggtag cactggatga
     1081 ggcagatggc ttccgccgcg atgccttcgc cacggccggt gaagccgagc ttctcggtgg
     1141 tggtggcctt gacgttcacg gtatcgagca agatctggca atcggctgcg atattggcgc
     1201 gcatctggtc gatataaggc gcgagcttgg gcgcctcggc gatcaccgtg gcgtcgatat
     1261 tgacgatgga atagtgcttg gcctccagca acttggccac atgcgtcagc aattgccgag
     1321 aatcgatgtc cttgtagcgt tcgtcggtgt tggggaaatg cttgccgata tcgcccagcg
     1381 ccgcggcacc aagcaaggcg tcgcaaatcg catgtaggag cacatcggca tcggagtgcc
     1441 catccagccc attttcgtgg gggatctcga ccccgccaat aacgcagcgt cggccttcca
     1501 ccaactgatg cacatcgaag ccctgtccta tcctgatcat gtcgttctcc ttgatgcttg
     1561 acgttggtta tccgcagtca ggatggcttc cgccatcgcc aggtcctgcg ggtatgtgac
     1621 cttgagattg cgtaactcgc ccggtaccag tttgggcctg aacccttgcg cctcgaccgc
     1681 ctgcgcttca tccgtcggcg caccgccagc cgcttgcagg gcctgccgca acagaccgta
     1741 acggaacatc tgcggcgtct gcgcctgcca caggttttcg cgcgactcgg tcttggccac
     1801 cctgctctcg gcatcggcgc gcttcaatgt atccgccagc gggatcgcca gtagcccgcc
     1861 gaccgcgtct tcctgcaagc tgtcgagcaa ccggtcaagc aaggcctgat tcaacccagg
     1921 gcgggccgca tcgtgcacca gcacccagtc ttcgtcaccc agctggccgg cgatggcctc
     1981 cagcccattg agcacggtcg cggcacgcgt ctcgccgcca caacgccaga tcttgatctt
     2041 cgcatccagc acaggcatca agcgctgcca atcgacatcg tcttcgctga gcacgacatg
     2101 gacgctggcg atgcgggggg aaacggaaaa aaccgacaag gtgtgggcaa tgagcggatg
     2161 cccgagcaag ggcaggtatt gcttgggtaa ttcggacccc atgcggctcc cggagccggc
     2221 tgcagggata atgacgtgaa aactggccat gccagaatct tagcatgtcg aaagtgcatg
     2281 tcggcgcgca gaatataagt cgaatgtatg taaacacctg cccttatggg catcgcagat
     2341 cccatgaaag cgcactgtcc agccatataa gtaacacgct gcgccttatg ggctttgccc
     2401 atgatcgact tcaacctatc ccgc
//
LOCUS       DATMTW010000386         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_536246, whole genome shotgun sequence.
ACCESSION   DATMTW010000386 DATMTW010000000
VERSION     DATMTW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536246"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VN084_07555"
     CDS             <1..876
                     /locus_tag="VN084_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWT29353.1"
                     /translation="AEETLRSQQDSYDLIQRKFELGLGSALDLRQAQTTVEAARVEIA
                     RYKSLVKLDRNALTLLVGTPLPQDLATPAALVPVSALAELPAGLPSEVLQQRPDVLQA
                     ERALRAANANIGAARAAFYPSISLTASVGSVSSSLSGLFGAGSGVWSFIPQISLPIFD
                     GGRNRANLTLSEVRKNIAIAQYEKAIQVAFREVADALAQHETMAQQLDAQTALVKASD
                     EGLQLSDARYRGGIDSYLNVLDAQRSLYAAQQNLITLKLTALSNQVTLYKVLGGGWQE
                     RTTAGGSAASDRSGQ"
     gene            918..2195
                     /locus_tag="VN084_07560"
     CDS             918..2195
                     /locus_tag="VN084_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HWT29354.1"
                     /translation="MSLKPQDKSSAPPSGVLLPTSTLLAMMLAALAMLGPFSVDTYLP
                     AFPNIEQSLGTDLIGVQQTLTAYMFAFAVMILWHGALSDAFGRRNIILVSLAVFAIGT
                     LGCAASHSIEYLWAFRVLQGVSAGAGVVVGRAIIRDLYADAEAAKLLSLVTMIFATAP
                     AAAPIFGGWVVKHYDWRTIFLAIFAYTAMLLWYCYKRLPETLPASQRTPFEPQFLWRS
                     YTQIFRSLAFQLKAGAIAFNFAGLFLYVAAAPVFITQHLGLGPDQFAWQFVPAVAGIF
                     LGALTANRLAGKVSIPRLLFLGYTLLIGAGIGNVLYHLYFPPAIPWSVLPLFFYTFGM
                     SVVAPILTLLVMDLFPEIRGTVASCQSFTQTMLGAAVSGLIAPFLVHSVLWLALGQLA
                     FALIGLCLWLGGRYHHYRHIVPAQEVNAWESVE"
     gene            complement(2292..2933)
                     /locus_tag="VN084_07565"
     CDS             complement(2292..2933)
                     /locus_tag="VN084_07565"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWT29355.1"
                     /translation="MATLRLGDTAPDFTQDSSIGPIAFHQWLGDSWGVLFSHPADFTP
                     VCTTELGATAKLAEEFKKRNVKALALSVDPVESHHGWIKDINETQGCTVNFPILADAD
                     KKVATLYDMIHPNSLANATVRSVFIIDPSKKIRLTLTYPASTGRNFNEILRVIDSLQL
                     TDSHSVATPANWKDGDDVVIVPSLQDPDVLKAKFPKGFKAVKPYLRMTPQPNK"
     gene            complement(3066..>3780)
                     /locus_tag="VN084_07570"
     CDS             complement(3066..>3780)
                     /locus_tag="VN084_07570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWT29356.1"
                     /translation="DLPTPIGTMRTYVHRPAAEGRYAAILFYSEIFQQTGPIERAAKI
                     MAGHGYAVLVPEVFHELNPIGTVLGYDDAGREKGNADKAAKDVQGYDSDNRAMIEWVQ
                     AQPWCSGKIGAMGFCIGGHLAFRAALQPEVKATACFYATDLHTQIIPNKAGQHSMERL
                     ADIQGELQMIWGKQDNHIPTAGRAQVYQQLTDAKLTFTWHEFNGQHAFMRDEGERYDP
                     ELQMLGYQLALSMFTRTLR"
BASE COUNT          647 a         1199 c         1160 g          774 t
ORIGIN      
        1 gcggaggaga cgctacgtag ccagcaggat tcctatgatc tcatccagcg caagttcgag
       61 ctcggactgg gttcggcgct ggatctgcgc caggcgcaga ccacggtgga ggcggcgcgc
      121 gtcgagatcg cgcgctacaa gagcctggtc aagctggacc gaaacgcttt aaccctgctg
      181 gtcggtacgc cgctgccgca ggacctggcg acaccggccg cgttggtgcc agtcagcgcg
      241 ctggccgaac tgccggccgg tctgccctct gaggtactgc agcaacgccc tgacgtactg
      301 caggccgagc gggcgctacg cgcggccaac gccaacatcg gcgcggcacg ggcagcgttt
      361 tacccgtcca tcagcctgac cgcgagcgtc ggcagcgtga gcagttcgct gtcgggcctg
      421 ttcggcgccg gttccggtgt ctggtccttc attccgcaga tcagcctgcc gatcttcgat
      481 ggcggccgca atcgcgccaa cctgacgctg tctgaggtgc gcaagaacat cgccatcgca
      541 caatacgaga aagccatcca ggtcgctttc cgcgaggtgg cagacgccct ggcgcagcac
      601 gagacgatgg cgcaacagct cgatgcccaa accgcgttgg tgaaggccag cgacgaaggc
      661 ctgcaactgt ccgacgcccg ttatcgcggc ggcatcgaca gctacctcaa cgtgctcgat
      721 gcccagcgca gcctctatgc cgcgcaacag aacctgatca cgctgaagct gacggccctg
      781 agtaaccagg tgaccctgta caaggtgctg ggcggcggct ggcaggagcg cacgacggcc
      841 ggcggcagcg cagccagcga caggtccggc caatgagcgc gatttccgcg gccagggctt
      901 cattgttata gttgcgcatg tcactcaaac cccaagataa atcatccgcg ccgccatccg
      961 gcgtcctgct gcccaccagc accctgctcg ccatgatgct ggcggccctt gccatgctcg
     1021 gcccgttctc ggtggatacc tacctgccgg cgttccccaa catcgaacaa tcgctgggta
     1081 ccgacctcat cggcgtgcaa cagacgctca ccgcctacat gttcgccttc gccgtgatga
     1141 tcctctggca tggcgctttg tcggacgctt tcggccggcg caacatcatc ctcgtctcgc
     1201 tggcggtgtt cgccatcggc acgctgggct gcgccgcctc gcacagcatc gaatatctgt
     1261 gggcgttccg cgtgctgcag ggggtctctg ccggtgccgg cgtagtggtg ggccgcgcca
     1321 tcatccgcga tctctatgcc gatgctgagg ccgccaagct gctctcgctg gtgaccatga
     1381 tcttcgccac cgcaccggcg gcggcgccga tcttcggcgg ctgggtggtc aagcactacg
     1441 actggcgcac catcttcctc gccatcttcg cctacaccgc catgctgctc tggtactgct
     1501 acaagcgact gcccgagacc ttgccggcat cgcagcgcac cccgttcgag ccacaattcc
     1561 tctggcgcag ttacacgcag atcttccgtt cgctggcgtt ccagctcaag gcaggcgcca
     1621 tcgccttcaa tttcgccggc ctgttcctgt atgtggcggc ggcacccgtc ttcatcaccc
     1681 agcatctcgg cctggggccg gaccagttcg cctggcagtt cgtacccgcc gtcgccggca
     1741 tcttccttgg cgcgctgacc gccaaccggc tggctggcaa ggtctccatc ccgcgcctgc
     1801 tgttcctcgg ctataccctg ctgatcggcg caggtatcgg caatgtgctc tatcacctgt
     1861 atttcccgcc ggccatcccg tggtcggtgc tgccgctgtt cttctacacc ttcggcatgt
     1921 cggtggtggc gccgatcctg accctgcttg tgatggacct gtttccggag atccgcggca
     1981 cagtggcctc ctgccaatcg ttcacgcaga ccatgctggg cgcggcggtc tccggcctga
     2041 tcgcgccatt cctcgtgcat tcggtgctat ggctggcgct gggccaactg gccttcgcgc
     2101 tgatcggcct ctgcctgtgg ctgggcgggc gttaccacca ttaccggcat atcgtcccgg
     2161 cacaggaagt gaatgcctgg gagagcgtgg aataacgaag gtcgatcctt caaaaggcct
     2221 tgcgggtccc aataaaaaag ccaccctgcg gtggcttttt tattgcaagg ggctctattg
     2281 cagcgaacag cctatttatt gggctgcggc gtcatgcgca gatagggctt cacggccttg
     2341 aagcctttgg ggaacttggc cttgagcacg tccggatcct gcagcgatgg cacgatcacg
     2401 acatcgtcgc cgtccttcca gttggcgggc gtggccacgc tgtggctatc ggtcagctgc
     2461 agtgaatcga tcacgcgcag gatctcgttg aaattgcgcc cggtactggc agggtaggtc
     2521 agggtcaggc ggatcttctt gctggggtcg atgatgaaca ccgaacgcac cgtggcgttg
     2581 gccagcgaat tggggtggat catgtcgtac agcgtggcga ccttcttgtc ggcatcggcg
     2641 aggatgggga aattcacggt gcaaccctgg gtctcgttga tgtccttgat ccagccgtgg
     2701 tgggattcca ccggatcgac cgataacgcc agcgccttga cgttgcgctt cttgaattcc
     2761 tcggcaagct tggcggtggc gccgagctcg gtggtgcaga ccggcgtgaa atcggccgga
     2821 tgcgagaaca gcacacccca gctatcgccc agccattgat ggaaggcgat ggggccgatg
     2881 ctggaatcct gcgtgaaatc aggggcggta tcgcccagac gtaatgtagc catttactgt
     2941 ttcctttcga tcctgagact agtgctacag catggcaccg aacggccatg cagggaaata
     3001 atctttgatt ttaaggatat agtgaaccgg catatagccg gttcactatg cgttcgatga
     3061 tttgcctagc gcagggtacg cgtgaacatc gacagcgcga gctggtagcc cagcatctgc
     3121 agttccgggt cgtaccgctc gccttcgtcg cgcatgaagg catgctggcc gttgaattcg
     3181 tgccaggtga aggtgagctt ggcgtcggtc aattgctgat agacctgggc gcggcctgct
     3241 gtcgggatat ggttgtcctg cttgccccag atcatctgca actcgccctg gatatcggcc
     3301 agccgttcca tgctgtgctg gcccgccttg ttggggatga tctgcgtgtg caggtccgtg
     3361 gcgtagaagc aggccgtggc cttcacttcc ggctgcaagg ccgcacggaa cgccaagtgg
     3421 ccgccgatac agaagcccat ggcgccgatc ttaccggagc accatggctg agcctgcacc
     3481 cactcgatca tggcacgatt gtcgctgtcg taaccctgca cgtccttggc cgccttatcg
     3541 gcattacctt tctcgcggcc ggcatcgtca taaccgagga cggtgccgat cgggttcagc
     3601 tcgtggaaca cttctggcac caatacggcg tagccatggc cagccatgat cttggcggca
     3661 cgctcgatcg gaccggtctg ctggaagatc tcggaataga acaggatggc cgcataacga
     3721 ccttctgccg ccgggcggtg gacataagtg cgcatggtac cgatgggcgt tggcaaatca
//
LOCUS       DATMTW010000387         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_570308, whole genome shotgun sequence.
ACCESSION   DATMTW010000387 DATMTW010000000
VERSION     DATMTW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570308"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VN084_07575"
     CDS             complement(<1..1251)
                     /locus_tag="VN084_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit TatB"
                     /protein_id="HWT29357.1"
                     /translation="MMENTLKRYAELEDKHVELLTELVAQMRPGRQQHSDHAVHAIQA
                     LCHVLHRHPEYVPLLRTAILTLIGKHKPVSLIADAGIHPNSGFFSELWRRIGHKFLPE
                     AVNPAYLKDLFAQIFNDPEDERWVLVVPDAVWIELMSALRLQEASPELLLACRKNLFD
                     AAQVLSYRLSALGLEPELIRHFPELEDHSSPFIIQNAEFGSFLAAPEEKLEAIRHILV
                     MLDQCRKVVARVRRSSSQTGTSIRLTFLLQRISQHIQRLELLLDIIARQVADEDVAAL
                     YVKLLKILVRAECHKNDVRQHWRENMELLALRVTENASRTGEHYITETRSEYFALMRS
                     AMGAGVIVGVMAMIKVITAGHHYAPLTEAIFFSLNYGLGFVLIHMLHFTVATKQPAMT
                     AAAIAASIDGSDGKSKNMDKLVTII"
     gene            complement(1373..2182)
                     /locus_tag="VN084_07580"
     CDS             complement(1373..2182)
                     /locus_tag="VN084_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWT29358.1"
                     /translation="MRSTTTTCLALMMTLGLGACAASKMEKQEAPADMNLAQPKDSNP
                     YIGNYTSRNRPSLGLQPEPGAPKLYRGVVETADYQRMLEDGYDLLGYSKFEANTVSPE
                     LSLEQAKQLKADVVLVYSKVVGNVPATVQLQQMRERARQHTDADKATKQKAMGDVLAE
                     AEDRQLYEYFATYWAKLAPPLIGVHITSLKDEDRKSEVGLDVLAVIKRSPADKAHIES
                     GDVLLKIGDVALNKPELLSQAASRYAGQTVEVSYLRGNKPFKTTMTLNKPQ"
     gene            2346..2963
                     /gene="mog"
                     /locus_tag="VN084_07585"
     CDS             2346..2963
                     /gene="mog"
                     /locus_tag="VN084_07585"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HWT29359.1"
                     /translation="MNATPLRIGLVSISDRASSGVYEDKGIPTLKAWLERALTSPWES
                     ISRVIPDEQGEIESTLCDLVDHEGCHLVLTTGGTGPALRDVTPEATLAVGDRVMPGFG
                     EQMRQISLHFVPTAILSRQVAVTRKQSLIINLPGQPKSIAQTLEGLKDEAGNILVHGI
                     FAAVPYCLDLLGTDKHPMPYLETDGDIVKAFRPKSAARPAADPAS"
     gene            2963..3934
                     /gene="thiL"
                     /locus_tag="VN084_07590"
     CDS             2963..3934
                     /gene="thiL"
                     /locus_tag="VN084_07590"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWT29360.1"
                     /translation="MASEFDLIRRYFTRPTPGAVLGVGDDAALLKVSDGMELAISADM
                     LVAGTHFFADTDAYLIGWKSLAVNVSDMAAMGAVPKWATLAIALPDADEAWVRRFADG
                     FFACASRYQIELIGGDTTRGPLAISVQIMGEVAAGKAMRRAGARVGDEVWVSGTLGSA
                     ALALAALQGRYPLSDDEREACLPALHRPQPRVELGLLIGASAHSAIDISDGLLADLGH
                     ILRASNVGAEIRLRDIPVSRAVSAHFADKTVQQMVLAGGDDYELCFTASARQHGEIAK
                     LTSLLALPLTCIGHITEAPGLIVRGLDDAPLELGMHGYDHFSDKAAS"
     gene            3931..4425
                     /locus_tag="VN084_07595"
     CDS             3931..4425
                     /locus_tag="VN084_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HWT29361.1"
                     /translation="MSTRPDLRFMLRNPMHLLALGGGSGLSPIAPGTMGTLLAAPLFL
                     VLMQTPEMAHYAIIAGLFLIGIPACGLTGKHLGVADHGSIVWDEIVAFMLVLEFAPLS
                     WQWWLTAFLLFRLFDIWKPFPISYFDARLKGGFGVMFDDLLAAVYAVACMKGLQWWML
                     NSSI"
     gene            4401..4898
                     /locus_tag="VN084_07600"
     CDS             4401..4898
                     /locus_tag="VN084_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HWT29362.1"
                     /translation="MDAELFDLSRALGVALKERQWLLALAESCTGGWAAESVTAVPGS
                     SAWFDCGFVTYSNTAKQEQLGVTEQTLELHGAVSEHTALEMALGALHHSHADITAAIT
                     GIAGPDGGTEEKPVGTVCFAWATAEGVAESVTLHFSGDREAVRRASVKTVLEGLLQLT
                     LATDL"
     gene            4946..5977
                     /gene="recA"
                     /locus_tag="VN084_07605"
     CDS             4946..5977
                     /gene="recA"
                     /locus_tag="VN084_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWT29363.1"
                     /translation="MDDNKSKALAAALSQIEKQFGKGSIMRMGDTDVAGDIQAVSTGS
                     LGLDIALGIGGLPRGRIVEIYGPESSGKTTLTLSVIAQMQKLGGVAAFIDAEHALDPQ
                     YAQKLGVNVSELLISQPDTGEQALEIADMLVRSGSVDIVVVDSVAALTPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRTGAIKKGDEVIGSETRVKVVKNKVAPPFKQAEFDILYGEGISREGEILE
                     MGSNLKLVEKAGAWYSYKGEKIGQGKDNAREYLKEHPEVADEIDAKIRDNAKVITAAV
                     APVDNESDD"
     gene            5958..6425
                     /gene="recX"
                     /locus_tag="VN084_07610"
     CDS             5958..6425
                     /gene="recX"
                     /locus_tag="VN084_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HWT29364.1"
                     /translation="MTNLTTKPVISLRERALGYLSRREYSCKELAQKLSAYAEPEDDV
                     PALIEDFKQRGWLSEERYAEQIVHARKSKFGSMRVAHELREHGVAEELVEQALAEVRQ
                     DELGNARAVWQKKFKTAPKNREEWAKQARFLQGRGFSFELIKKVLNDTPDETI"
     gene            6447..>6789
                     /locus_tag="VN084_07615"
     CDS             6447..>6789
                     /locus_tag="VN084_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWT29365.1"
                     /translation="MNSKPSSKEIRQAFLDYFASKGHQVVASSSLVPVGDPTLLFTNA
                     GMNQFKDVFLGFDKRPYTRAASSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFG
                     DYFKKDAIHFAW"
BASE COUNT         1339 a         2130 c         2045 g         1275 t
ORIGIN      
        1 gatgatggtc accagcttgt ccatgttctt cgatttgcca tcgctgccgt cgatgctggc
       61 ggcgatcgcg gccgccgtca tggctggctg tttggtcgcc accgtgaaat gcagcatgtg
      121 gatgagcacg aatccgagac cataattgag actgaagaag atcgcctcgg tcagcggtgc
      181 gtagtggtgg ccagcggtga tcaccttgat catggccatg acgccgacga tgacaccggc
      241 acccatcgcg gagcgcataa gcgcgaaata ctcgctgcga gtctcggtga tgtaatgctc
      301 gccagtgcgg ctggcgttct cggtcacacg caacgccagc agctccatgt tctcgcgcca
      361 gtgctgacgc acgtcgttct tgtggcactc ggcgcgcacc aggatcttca gcagcttgac
      421 atagagcgcc gcgacatcct cgtcggccac ttgccgggcg atgatatcga gcaacagttc
      481 caggcgctgg atatgctgac tgatgcgctg caacaggaac gtgagacgga tgctggtacc
      541 ggtctgagag ctcgaacggc gcacgcgcgc gacgaccttg cggcactggt ccagcatcac
      601 caggatatgg cgaatggctt cgagtttctc ttccggcgcg gcgaggaaac tgccgaactc
      661 ggcgttctgg atgatgaaag gcgagctatg gtcttccagc tccggaaagt gacggatcag
      721 ctctggctcc agtcccagcg ccgacaagcg gtacgagagc acctgggccg catcgaacag
      781 gttcttgcga caggccagca gcaactccgg cgacgcctcc tgcagacgca atgcactcat
      841 cagttcgatc cagaccgcat cgggcaccac cagtacccag cgttcatcct cggggtcgtt
      901 gaagatctgg gcgaacagat ccttcaggta agctgggttg accgcctccg gcagaaactt
      961 gtggccgatg cggcgccaga gctcgctgaa gaagccgctg ttcggatgga tgccggcatc
     1021 tgcgatcagc gagaccggct tatgcttgcc gatcagggtc aatatcgccg tgcgcagcag
     1081 gggcacgtat tccggatggc ggtgcaacac atggcataag gcctggatgg cgtgcaccgc
     1141 gtgatccgaa tgttgctgcc ggccaggacg catctgcgcc accagttcgg tgagcagctc
     1201 gacatgcttg tcctcgagct cggcataacg tttgagggtg ttttccatca tgatcttgtc
     1261 ttgggcggga cgctagattt aaccacgacc ttccgaggaa agccaaacgg ctagagataa
     1321 ggcgaagtgc ggcgcgcccg accgccgatg ggatgcgacg ggcgcgatag ggttattgcg
     1381 gcttgttgag cgtcatcgtg gttttgaagg gtttgttgcc ccgcaaatag ctcacttcca
     1441 cggtctggcc ggcataacgg ctggcagcct ggctcagcaa ttccggcttg ttcagcgcga
     1501 catcaccgat cttcagcagc acgtccccgc tttcgatatg ggccttgtcc gccggcgatc
     1561 gcttgatcac cgccagcacg tcgagcccga cctcgctctt gcggtcttcg tccttgaggc
     1621 tggtgatatg gacgccgatc aaaggtggcg cgagcttggc ccagtacgta gcgaaatact
     1681 cgtagagctg gcggtcttcc gcttcagcca gcacatcgcc catggccttc tgcttggtcg
     1741 ccttgtcggc gtccgtatgt tgccgggcac gctcgcgcat ctgttgcagc tgcacagtgg
     1801 ccgggacgtt acccacgacc ttgctgtaga ccagcacgac atccgccttc agttgcttcg
     1861 cctgctccag cgacagttcg ggcgacaccg tattggcttc gaatttggaa tagccgagca
     1921 ggtcgtagcc atcttccagc atgcgctggt agtcggctgt ctcgaccaca ccacgataga
     1981 gcttcggcgc gccaggctca ggctgcaaac cgagcgaggg gcgattgcgg ctggtgtaat
     2041 ttccgatgta ggggttgctg tccttcggct gcgccagatt catgtcggca ggcgcttcct
     2101 gcttctccat cttgctggcg gcacaggcgc cgaggccaag cgtcatcatc aaggccaggc
     2161 atgtagtggt ggttgaacgc atctgtatcc ctcttcgata ttcgcggcaa ctggcaatcc
     2221 tgcatatgac gccgtcgcct catgttggtg agcgtaatga tatgattagc gtatctgccg
     2281 gcggttccag ccgagagaca aatattacgc cgcaaccact acgtccggac atccacacga
     2341 cccatatgaa cgccacacct ttacgcatcg gcctcgtctc catcagcgat cgcgcttcga
     2401 gcggcgtcta cgaggacaag ggcatcccta cgctcaaggc atggctggag cgggcactga
     2461 ccagcccctg ggaatcgatc agccgcgtca tcccggacga acagggcgag atcgaatcga
     2521 cgctctgtga ccttgtcgac catgagggct gccacctggt gctgactacc ggcggtacag
     2581 gcccggcgct gcgcgacgtc acgccggaag cgacactggc cgtcggcgat cgtgtcatgc
     2641 cgggcttcgg cgaacagatg cgccagatca gcctgcattt tgttccgacc gccatcctct
     2701 cgcgccaggt cgccgtcacc cgcaagcaga gcctgatcat caacctgccg gggcagccaa
     2761 aatccatcgc gcagacactc gaaggcctca aggacgaggc tggcaatatc ctcgtgcatg
     2821 ggatcttcgc agcggtgccc tactgcctgg acctgctcgg caccgacaag caccccatgc
     2881 cctacctcga aaccgacggc gacatcgtca aggccttccg ccccaaatcc gcggcaagac
     2941 ctgccgccga cccggccagc tgatggcttc ggaattcgat ctcatccgcc gctatttcac
     3001 gcgccctacg ccgggcgcgg tgctcggtgt aggcgatgac gccgccctgc tcaaggtcag
     3061 cgatggcatg gagctggcca tctcggctga catgctggtc gcggggacgc acttcttcgc
     3121 ggataccgac gcttatctca tcggctggaa atcgctggcg gtcaatgttt cggacatggc
     3181 cgccatgggc gccgtaccga aatgggcgac gctcgccatc gccctgcccg atgccgacga
     3241 ggcttgggtg cggcgattcg cagatggctt cttcgcctgc gccagccgct accagatcga
     3301 gctcatcggc ggcgacacca cacgcggacc gttggccatc agcgtgcaga tcatgggcga
     3361 agtcgctgcc ggcaaggcca tgcgccgtgc cggcgccagg gtcggtgacg aagtctgggt
     3421 gtccggcacg ctgggcagcg ccgcgctggc gctcgctgcc ttgcagggcc gctatccgct
     3481 cagtgacgat gagcgcgagg cctgcctgcc ggccttgcat cgaccacaac cacgggtcga
     3541 gcttggcttg ctgatcgggg ccagcgcgca cagcgccatc gacatctctg atggcttgct
     3601 ggccgatctc ggccacatcc ttcgcgcatc aaacgtgggc gcagagatcc ggctccgcga
     3661 catcccggtc tcgcgtgcgg taagcgccca cttcgccgac aagactgtgc agcagatggt
     3721 gctggccggc ggcgatgact acgaactgtg cttcaccgca tcggcgcgac aacatggcga
     3781 gatcgccaaa ctgaccagcc tgctcgccct gccgctgacc tgcatcggcc acatcaccga
     3841 ggcgcccggc ctcatcgtac gcggccttga cgatgcgccg ctcgagttag gcatgcacgg
     3901 ttacgaccat ttctccgata aggccgcctc atgagcacac gtcccgatct gcgcttcatg
     3961 ctccgcaacc ccatgcacct gctggcgctg gggggcggca gcggcctctc ccccatcgcc
     4021 cccggcacca tgggcaccct gctggccgcg cccctgttcc tggtactcat gcagacaccg
     4081 gaaatggcgc attacgcgat cattgccggc ctcttcctga tcggcatccc ggcctgtgga
     4141 ctcaccggca aacacctcgg cgtcgccgac cacggcagca tcgtctggga cgagatcgtc
     4201 gcgttcatgc tggtgctgga attcgccccc ctcagctggc aatggtggct cacggccttt
     4261 ttgctgttcc gtctgttcga tatctggaaa cccttcccca tcagttattt cgacgcgcgc
     4321 ctcaagggcg gctttggcgt catgttcgac gacctgctgg ccgcggtgta tgcggttgcc
     4381 tgcatgaaag gattgcagtg gtggatgctg aactcttcga tctgagccgc gcgctgggcg
     4441 tcgcgctcaa ggaacgccaa tggctactgg cgctggctga atcctgcact ggcggctggg
     4501 ccgctgaatc cgtcaccgcg gtgccaggta gctcggcctg gttcgactgc ggcttcgtca
     4561 cctacagcaa caccgccaag caggaacagc tcggcgtcac cgaacagacc ctggagctgc
     4621 atggtgcagt gagtgagcac acggcgctgg aaatggcgct gggcgcattg catcacagcc
     4681 acgccgacat caccgccgcg atcacgggta tcgccggccc ggacggcggt accgaggaaa
     4741 agccggtagg cacggtttgc ttcgcgtggg cgaccgcgga aggggtcgcc gaaagcgtca
     4801 ccctgcactt ttccggagac cgcgaagcgg tgcgccgggc ctcggtaaaa accgtgctgg
     4861 aagggctgtt gcagcttacc ttagctactg atttatgaaa taattatgcg ctttatgaag
     4921 tgcacaaaaa caagggaccg aacacatgga tgacaacaaa agcaaagcgc tggcagcggc
     4981 actctcgcag atcgagaagc aattcggcaa aggctccatc atgcgcatgg gcgataccga
     5041 cgttgccggc gacatccagg ctgtctccac tggctcgcta gggctcgata tcgcgctcgg
     5101 catcggcggc ttgccgcgtg gccgcatcgt cgagatctac ggtccggaat cctcaggcaa
     5161 gaccaccctg accctctccg tcatcgccca gatgcagaaa ctgggtggcg tcgccgcctt
     5221 catcgacgcc gaacacgcgc tcgatccgca atatgcgcaa aagctcggcg tcaacgtctc
     5281 agagctattg atctcacagc cggataccgg cgagcaagcg ctcgagatcg ccgatatgct
     5341 ggtgcgttcc ggttcggtcg atatcgtcgt cgtcgactcc gtcgccgcgc tgacgcccaa
     5401 ggccgagatc gaaggcgaga tgggcgattc gcacatgggc ctgcaggcgc gcctgatgtc
     5461 gcaggcgctg cgcaagctca ccgccaacat caagcgcacc aacacgctgg tcatcttcat
     5521 caatcaaatc cgtatgaaga tcggcgtcat gttcggcaat cccgagacca ccaccggcgg
     5581 taacgcgctc aagttctatg cctccgtgcg tctcgatatc cgtcgcaccg gcgccatcaa
     5641 gaagggggac gaagtcatcg gctccgagac ccgcgtcaag gtcgtcaaga acaaggtcgc
     5701 gccgccgttc aagcaggccg agttcgacat cctctacggc gaaggcatct cgcgcgaagg
     5761 cgagatcctc gagatgggct ccaacctcaa gctggtggag aaggccggcg cctggtacag
     5821 ctacaagggc gaaaagatcg gccagggtaa ggacaatgcc cgcgaatacc tgaaggaaca
     5881 tccggaagtc gccgacgaga tcgacgccaa gatccgagac aatgccaagg tgatcacggc
     5941 ggctgtagcc ccggtggata acgaatctga cgactaagcc tgtcatcagt ctgcgcgaac
     6001 gtgcgttagg gtatctctcc cggcgcgagt attcctgcaa ggagctggcg cagaagctca
     6061 gtgcctatgc cgagccggaa gacgatgtgc cggccctgat cgaagacttc aagcagcgcg
     6121 gctggctctc cgaagaacgt tatgccgaac agatcgtgca tgcgcgcaag agcaagttcg
     6181 gcagcatgcg cgtggcacat gagctgcgcg aacatggcgt ggccgaggaa ctggtcgaac
     6241 aggcgctggc ggaagtgagg caggacgaat tgggcaatgc ccgcgccgta tggcaaaaga
     6301 aattcaagac cgccccgaag aaccgcgagg aatgggccaa gcaggcgcgg ttcctgcaag
     6361 gccgcggatt cagcttcgag ctgatcaaga aagtactgaa cgatacaccg gacgaaacca
     6421 tttaagcgtg agcgtgaaag cgattgatga acagcaagcc aagcagtaaa gagatccgcc
     6481 aggcctttct ggactatttt gcctcgaaag gccatcaggt cgtggcatca agctcgctcg
     6541 tacccgtcgg cgatcctaca ctgctgttca ccaacgccgg catgaaccag ttcaaggacg
     6601 tcttcctcgg cttcgacaaa cgtccctata cccgcgccgc ttcaagccag aagtgcgtgc
     6661 gcgccggcgg caagcacaat gacctcgaga acgtcgggta tacggcacgc caccacacct
     6721 tcttcgagat gctgggcaat ttcagtttcg gcgattactt caagaaagac gccatccact
     6781 ttgcctggg
//
LOCUS       DATMTW010000388         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_234844, whole genome shotgun sequence.
ACCESSION   DATMTW010000388 DATMTW010000000
VERSION     DATMTW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234844"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VN084_07620"
     CDS             complement(<1..125)
                     /locus_tag="VN084_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWT29366.1"
                     /translation="MKAMILAAGRGERMRPLTDVTPKPLLEVGGKPLIVWHLERL"
     gene            complement(125..1120)
                     /locus_tag="VN084_07625"
     CDS             complement(125..1120)
                     /locus_tag="VN084_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWT29367.1"
                     /translation="MQTRSEQLQSWLSEVLPQGGHSLSPASADASFRRYFRVDLGDRT
                     LIVMDAPPAHEDCEPFVRIAQLFLKAGLHVPQVLAQDLRQGFLLLTDLGTRTYLEALA
                     PENAGDMYREAYQALVTLQLASKPGVLPDYDEALLTREMQLFPDWYVSRHLGRELTAA
                     QQQVLGQVFATLNRNILGQGQVYVHRDYHSRNLMVCDTNPGVLDFQDAVHGAITYDLV
                     SLLKDAYIAWPEEQVLDWAVRYWQAARTAHLPVPADFSEFYRDFEWMGAQRHIKILGI
                     FARLYYRDGKENYLKDMPLVMDYLRRVCARYVELRPLLRLLDELHDSKPEVGYTF"
     gene            1258..>3655
                     /locus_tag="VN084_07630"
     CDS             1258..>3655
                     /locus_tag="VN084_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HWT29368.1"
                     /translation="MVKTAVKTLTVALVFLIQVHAHAEETGTQVASEAAPPADIITPD
                     TAIVEGDKLELYFDRKLKSIGNAAIHKADQNIYGDLIEYDVQNENVHVLGNAKLELND
                     SEINGPELNMKIGETIGEMKNPKFTLKKQFSNILQLGKSRNTTVDANTTTVTDARTGV
                     SQQQYDDDSMDDGLGVGQRAGVSRGDASVVFFEGENKKRLKDARYTTCSADSDDWYLK
                     ASELEIDGYTKTVVAHHATVEFQGVPILYTPWMNFSFANQRQSGFLAPTWGSTSLSGF
                     QIVTPYYWNIAPNMDATFGPRYLSKRGVQFVSEFRYLGETYSGIDNIEYLPSDSTTGA
                     TRYYAKLQHQQTFGGGLSGGYNLEKTSDDKYFSENLYFSRLSTGISSTSRVILPQNAY
                     LNYRFGDNWNLNGLVSKYQTLDNLSFPYERLPQLTLTNNQDFGPATFNLTSQWVQFDR
                     DQRAPIAVTGSRFVANPSVSTTFARPYGYITPKVGLHYTDYKLNDASIAQNGVANYDS
                     TNRTLPTFSLDSGLFFDRNTRIVNTRYTQTLEPRLFYVYIPYKDQSNLPIFDTSEATL
                     SYGSLFLENQFTGYDRVNDANQVTMALTTRMIESRTGEQRLAASIGQRFYFSDQRVTL
                     PGQVPSGDNTSDYFAAITARLKTHWNVDLAGQYSSDRRKLVRSNISARYNPEPGKAFN
                     FGYRYAESRLATGGVTSLCTIDPRYSCGLDQLNVSAQWPLGRGFFGLGRWNYSLEESK
                     PIEALAGLEYDAGCWQARGVLQRIATPTADANYGIFFQLELGGLASIGQNPLRLLTRG
                     I"
BASE COUNT          830 a         1181 c          963 g          681 t
ORIGIN      
        1 ctcagtcgtt ccagatgcca tacgatcaac ggcttgccac cgacctcgag caagggtttc
       61 ggcgtcacat cggtcagagg gcgcatacgt tcgccacgcc cggcggccag gatcatggcc
      121 ttcatcagaa ggtatacccg acttcaggct tgctgtcgtg aagctcgtca agcagacgca
      181 acagtggtcg caattcgaca taacgcgcac agacacgacg caggtaatcc atcaccagcg
      241 gcatatcctt aaggtaattc tccttgccat cgcgataata gaggcgggcg aatatcccca
      301 gtatcttgat atggcgctgc gcgcccatcc actcgaaatc ccgatagaac tcggagaaat
      361 cagccggcac cggcaagtgt gccgttcttg cggcctgcca gtagcgtacg gcccaatcca
      421 gcacctgctc ttccggccat gcgatgtaag catccttgag cagcgacacc aggtcgtagg
      481 tgatcgcgcc atggacggca tcctgaaaat ccagcactcc cgggttggtg tcgcatacca
      541 tgaggttgcg ggaatggtag tcgcggtgca catacacctg cccctggcca aggatgttgc
      601 ggtttagcgt ggcgaacact tgccccagca cttgctgctg cgctgccgtc aactcccggc
      661 ccagatgccg cgacacatac cagtcgggga agagttgcat ctcgcgtgtg agcagcgcct
      721 cgtcgtaatc cggaagcaca cccggcttgc tggccagttg cagcgtcacc agtgcctggt
      781 aggcctcgcg atacatgtca ccggcgttct ccggcgccag ggcctcgaga tacgtacgcg
      841 taccaagatc ggtcagcaac aggaacccct gccgcaagtc ttgagcgagt acctgcggca
      901 cgtgcaaccc tgccttgagg aagagttgcg cgatgcgcac gaacggttcg caatcctcgt
      961 gggcgggcgg cgcatccatg acgatcaatg tccgatcgcc aagatcgacg cggaaataac
     1021 ggcggaagct cgcgtcggca gaagcgggcg agagcgaatg gccgccctgt ggcagcactt
     1081 cggatagcca ggattgaagt tgttcggatc tggtttgcat gaggttcccg ggcttggtgg
     1141 acacgcctgc gccatgcgcg gcggggcaaa ttatggattg ccatgacatc gaatttatgc
     1201 gattttatca cccacaaaag aaacacgcgc tcacagcctc gagctgaata aaattacatg
     1261 gtaaaaactg cagttaaaac cctcaccgtt gcgcttgtat tcttgatcca ggtccatgcc
     1321 cacgcagaag agacgggcac gcaagtcgca tccgaagccg cgcccccagc cgacatcatc
     1381 accccggaca ccgccatcgt cgagggcgac aagcttgagc tatatttcga ccgcaaactg
     1441 aaatccatcg gcaatgcggc gatccacaaa gccgaccaga acatctacgg cgacctcatc
     1501 gaatacgatg tgcagaacga gaatgtccac gtcctgggta atgcgaagct cgaactgaac
     1561 gatagcgaaa tcaacggccc cgagctcaac atgaagatcg gcgagaccat cggcgagatg
     1621 aagaacccca agttcacgct gaagaaacag ttctccaata tcctgcagct gggcaaatcg
     1681 cggaatacga ccgtggacgc caacactact acggtcaccg atgcgcgcac cggcgtcagc
     1741 cagcagcagt acgatgacga cagtatggat gacggcctag gggtcggcca gcgcgccggc
     1801 gtctcgcgtg gggatgccag cgtggtcttt ttcgaaggcg agaacaagaa acgtctgaaa
     1861 gatgcccgct ataccacctg ctccgcagac agcgacgatt ggtacctgaa ggcgtctgaa
     1921 ctcgagatcg acggctacac caagacggtg gtcgcacacc atgccacggt cgaattccag
     1981 ggtgttccca tcctctatac gccatggatg aacttctctt tcgccaacca gcgccaatcc
     2041 ggtttccttg ctccgacctg gggttcgacc tcgctgagcg gcttccagat cgtcacgccg
     2101 tattactgga acatcgcacc gaacatggac gcgaccttcg gcccgcgcta cctgagtaaa
     2161 cgcggcgtgc aattcgtcag cgagttccgc tacctcggcg agacctattc gggcatcgac
     2221 aacatcgaat acctgccgag cgacagcacc accggcgcta cccgttatta cgccaagctg
     2281 caacatcagc agaccttcgg cggcggcctt tcaggcggtt acaatctgga aaagacctct
     2341 gacgacaaat acttttccga gaacctctat ttctcgcggc tatccaccgg tatcagcagc
     2401 accagccgcg tgatcctgcc gcaaaacgct tacctcaact accgtttcgg cgacaactgg
     2461 aacctcaacg gcctggtcag taagtaccag accctggaca acctgtcttt cccttacgaa
     2521 cggctaccgc agctgaccct gaccaacaac caggatttcg gcccggcgac gttcaacctg
     2581 accagtcagt gggtgcaatt tgaccgcgac caacgcgccc cgatcgcggt caccggtagc
     2641 cgcttcgtgg ccaaccccag cgtcagcacg accttcgccc ggccctatgg ctatatcacg
     2701 ccgaaagtcg gcctgcacta caccgactac aagctgaacg acgcaagtat cgcccagaac
     2761 ggtgtggcaa attacgattc taccaatcgc accttaccga ccttcagcct cgatagcggc
     2821 ctcttcttcg accgcaatac tcgcatcgtc aacacccgct acacccagac cctcgagcct
     2881 cgactgttct atgtctacat cccctacaag gaccagagca acctgccgat cttcgatacg
     2941 tcggaagcga cgctgtctta cggctcccta ttcctcgaga accagttcac tggttacgat
     3001 cgtgtcaacg acgccaacca agtcaccatg gcactgacga cgcgcatgat cgagtcgcgt
     3061 accggcgagc aacgcctcgc ggcctcaata ggccagcgct tctacttcag cgaccagcgt
     3121 gtcaccttgc caggccaagt accctcgggc gataacacct cggattactt cgcggccatc
     3181 accgcacgcc tgaagaccca ctggaacgtg gaccttgctg gccagtacag tagcgaccgt
     3241 cgcaagctgg tgcgctccaa catctccgca cgctacaacc cggagcccgg caaagctttc
     3301 aacttcggct accgttatgc agagagtcgt ttggcaacag gcggtgtcac tagcctctgt
     3361 acaatcgatc cacgctactc ctgcggcctc gaccagctca acgtctcggc ccagtggccg
     3421 ctcggtcgcg gattcttcgg cctggggcgc tggaactatt cgctggaaga atcgaaacca
     3481 atcgaagccc ttgccggtct agaatatgac gcgggctgct ggcaagcgcg cggcgtgctg
     3541 cagcggatcg ccacaccgac cgccgatgcc aactacggta tcttcttcca gctcgagctg
     3601 ggtggcctag cctccatcgg ccagaaccca ttgcgcctgc tgacccgcgg catcc
//
LOCUS       DATMTW010000389         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_100545, whole genome shotgun sequence.
ACCESSION   DATMTW010000389 DATMTW010000000
VERSION     DATMTW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100545"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..505)
                     /locus_tag="VN084_07635"
     CDS             complement(173..505)
                     /locus_tag="VN084_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29369.1"
                     /translation="MPTPSELDTLLQFRMVITPSPVYRANSQKVLRIRWIPEVWHVDR
                     GEVWQGEAVGDHAAAYAAAEAEWQVIVEAAFRNEFEEWYDDLPPAERKELDSRGAIQS
                     ELDRSREK"
     gene            complement(518..745)
                     /locus_tag="VN084_07640"
     CDS             complement(518..745)
                     /locus_tag="VN084_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWT29370.1"
                     /translation="MLYDEMYQRILVYQFVNGRDPQQIVLSPRRYAAWRAMVSKMYRD
                     SDVPQPSTGEPDSFCGIQVRCCELVPDIITR"
     gene            complement(745..2961)
                     /locus_tag="VN084_07645"
     CDS             complement(745..2961)
                     /locus_tag="VN084_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HWT29371.1"
                     /translation="MAPQLVESWLPGGKYRGREYLIGDLRGSPGESLSINVDTGKWCD
                     FAAGMRGGDMISLYAAINSLSQIDAARRLAEDHHVASAPARPVARAAEPTMRLGKPPQ
                     GTPVPTRYRRQSGGRAGEMVDLVVDVLDAYPDVDGQPIMYIGRIEARGDRSKVIVQLS
                     WNLDAGEWWGAGFPDPRPLFGLDLLAARPDAPVMVVEGEKTAKAARSLIGHAYVVVTW
                     PGGTNAWQKADWTPLHGRKALFFPDADAPGVNCMGSIIRAHHEKLKQTKVVDTTGMPE
                     GWDLADAVTEGWTTAKLVEWAKPRAKGYVPVAAAPAPVSVPAPVVPDVQVTVMKPQVI
                     EAEESDDVDPLPGSAFAAQADLGLAVNNKGTPHANLDNMARILKNWEPFRHICWFDTF
                     HQRKFTKRGGKVREWTEEDDIAITEIVQRDIGIARMGLETVRQAIQKMAMAQPKDEPR
                     DWMKSLVWDGVKRISRFCPDYLGSADTEYARGVGRNFFVSMVARTFKPGCKVDNMLVL
                     EGAQGARKSTALEAVGGAWFCEVAAEIGSQKFIEQIQGKLLVEIAELDSLNKADVSKI
                     KQAITCRDDRFRPSYGRYAKDHPRRCVFTGSTNEDRYLRDSTGARRFWPIKCGTVDAA
                     KIKADREQMFAEAVAEFMANSDWYQMPGEETAREQESRRSADSWEEILADYLEPRRAR
                     EMTMSAILQEAMGMDPMKQTRAEQQRAAGCMRVLGWVCEQKWVDGASRRVWKKVKR"
     gene            complement(2997..3314)
                     /locus_tag="VN084_07650"
     CDS             complement(2997..3314)
                     /locus_tag="VN084_07650"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HWT29372.1"
                     /translation="MPSEKAIMHDIMLAVSKAGHRVWRNNVGVATTRDGSVIRFGLAV
                     GSSDLIGVTKDGRFLAIECKTDSGRATDEQLNFISLVRSMGGRAGIARSISDAFTIIE
                     GLQ"
BASE COUNT          611 a         1128 c         1041 g          651 t
ORIGIN      
        1 tacatggaat atgtctacga ccacgagggg gactgacccc tcgccaccaa cgaattgccc
       61 caggggtgag cctggggttt cgggtcttgt tatcgtggac cagtccgtac tggttaggag
      121 agccaggttc ctatggttgc gagccctcgg gcatctgcac ctggccctag tgttacttct
      181 cgcggctgcg gtcaagctct gactggatgg ctccacggct gtccagctcc ttgcgctcgg
      241 ctggcggcag atcgtcatac cactcctcga attcgttgcg gaacgccgcc tccacgatca
      301 cctgccactc ggcctcagcc gcagcgtagg ctgcagcatg gtcgccgact gcttcaccct
      361 gccagacctc accgcgatcg acgtgccaca cctcggggat ccagcggatc cggagcacct
      421 tctggctgtt cgctcgatac accggtgacg gcgtgatcac catgcggaac tggagcagcg
      481 tgtcgagttc ggatggcgtt ggcatatgac ctccaggtta gcgggtgatg atgtcgggaa
      541 cgagttcgca gcagcggact tgaatgccac agaaggaatc tggctcccca gtcgacggct
      601 gagggacgtc actgtctcga tacatcttgc tcaccatcgc ccgccaagcc gcatacctcc
      661 gcggcgacaa cacgatctgc tgcggatcgc gtccgttcac gaactggtag accaggatgc
      721 gctgatacat ctcgtcgtag agcattagcg tttcaccttc ttccagacgc gtcgcgacgc
      781 gccgtcgacc cacttctgct cgcagaccca gcccaggacg cgcatgcatc cagcggcgcg
      841 ctgctgttcg gcgcgcgtct gcttcatggg atccatgccc atggcctcct ggagaattgc
      901 agacatggtc atctcgcgag cccggcgcgg ctccaggtaa tcggccagga tttcctccca
      961 cgagtcggca ctgcggcggg actcctgctc acgggccgtc tcctcgcctg gcatctgata
     1021 ccagtcggag ttggccatga attcggccac ggcttcagcg aacatctgct cgcgatccgc
     1081 cttgatcttc gcggcgtcga ccgttccgca cttgatcggc cagaagcggc gtgcccccgt
     1141 cgagtcgcgg agataccggt cctcgttcgt tgacccggtg aacacgcagc gccgcgggtg
     1201 atccttggca tagcgtccat aggatgggcg gaaccgatca tcgcggcagg tgatcgcctg
     1261 cttgatcttg ctgacgtccg ccttgttcaa cgagtccagt tcggcgatct cgaccaggag
     1321 cttgccctgg atctgctcaa tgaatttctg gctgccgatc tcagcggcga cctcgcagaa
     1381 ccacgcgcca ccaacggcct ccaaggctgt ggacttccgc gcgccctgag cgccttcgag
     1441 caccagcatg ttatcgacct tgcagcccgg cttgaacgtg cgggcgacca tcgagacgaa
     1501 gaagttgcgg ccgacgccgc gggcatactc cgtgtccgca ctgccaaggt agtccggaca
     1561 gaaccgactg atgcgcttca cgccgtccca gaccagggac ttcatccagt cgcgcggctc
     1621 gtccttcggc tgggccatgg ccatcttctg gatcgcctgg cgtacggtct ccagacccat
     1681 gcgagcgatg ccgatgtcgc gctggacgat ctcggtgatg gcaatgtcat cctcttcggt
     1741 ccactcacgg accttgccgc cacgcttggt gaacttgcgc tggtggaacg tatcgaacca
     1801 gcagatatgg cggaacggct cccagttctt caggatacgg gccatgttat cgaggttcgc
     1861 gtgcggcgtg cccttgttgt tgaccgccag gccgaggtca gcctgagccg cgaacgcgct
     1921 gccaggtagc gggtcgacgt cgtcagactc ctcggcctcg atcacctgcg gcttcatgac
     1981 ggtgacctgg acatcgggca ccacgggtgc cggcacggac acgggagcag gggcggccgc
     2041 cacgggcaca taccccttcg cccgcggctt ggcccactcg accagcttcg ccgtcgtcca
     2101 gccttcagtc acggcgtcgg cgagatccca gccctcgggc atgccagtgg tatcgaccac
     2161 cttggtctgc ttcagcttct cgtggtgggc gcgaatgatc gaccccatgc agttcacgcc
     2221 aggagcgtct gcatcaggga agaacagcgc cttgcgcccg tgcagtggcg tccagtcggc
     2281 cttctgccag gcgttcgtgc caccaggcca agtcaccacg acgtaggcat gaccgatcag
     2341 ggagcgagcg gccttcgccg tcttctcgcc ctcgaccacc atcaccgggg catcaggacg
     2401 ggcagccagg agatcgaggc caaagagcgg acggggatcc gggaatcccg caccccacca
     2461 ttcgccggca tcgagattcc acgagagctg aacgatgacc ttggatcgat cgccacgcgc
     2521 ctcgatgcga ccgatgtaca tgatgggctg cccgtcgacg tccggatacg catcaaggac
     2581 atcgaccacc agatccacca tttcgcccgc cctgccccct gactggcggc gataccgggt
     2641 cggaaccgga gttccctggg gcggcttgcc gaggcgcatg gttggctcag cagcgcgagc
     2701 gacaggacga gctggggcag atgcgacatg gtggtcctcg gccaggcggc gtgcggcatc
     2761 gatctgggac agggaattga tcgcagcata cagcgagatc atgtcgccgc cacgcatgcc
     2821 agcggcgaag tcgcaccact taccagtgtc cacattgatc gataggctct ccccgggaga
     2881 accgcggagg tcgccgatca gatattcccg accgcgatac ttgccgccag ggagccacga
     2941 ctccacgagc tgcggagcca tgggcagcag cgtcgctgcg agactcggga aatcgatcat
     3001 tgcagccctt cgatgatagt gaacgcgtcc gagattgatc gggcgattcc ggcgcgtcca
     3061 cccatagagc ggacgagcga tatgaaattg agctgttcgt cggtggcgcg accggagtcg
     3121 gtcttgcact caatggcgag gaaccggccg tccttggtga cgccgatgag atcggagctg
     3181 ccgactgcga ggccgaagcg aatgacgctg ccatctctgg tcgtcgccac gccaacgtta
     3241 ttgcgccaga cccgatgccc agccttgctg acggccagca tgatgtcgtg catgatggct
     3301 ttctcagagg gcatcgggat cttccttccg cggtggtatc cgcacgccta gccgacacag
     3361 cttcgtctcg aattcccgct cgcgcttccg atatccagca ttcatcttcc gcaggcattc
     3421 gcggcaaacc g
//
LOCUS       DATMTW010000390         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_166249, whole genome shotgun sequence.
ACCESSION   DATMTW010000390 DATMTW010000000
VERSION     DATMTW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166249"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /gene="cobC"
                     /locus_tag="VN084_07655"
     CDS             <1..576
                     /gene="cobC"
                     /locus_tag="VN084_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HWT29373.1"
                     /translation="LNLYLVRHTTPDIAPGICYGQSDLDVAESYAHELEVVRSKLAAV
                     EPVAIFSSPLKRCSQLAEAIASVTCCDVQHDRRLMELHFGDWELLAWNDIPRGLVDVW
                     ADDHVMQAPPSGESFHALSLRVQDFLQEVSANHLGQNLVVMTHAGVIRALLAHALNMK
                     LADSFRLQIDYGSITQITVENGITRVAYVNR"
     gene            563..2029
                     /locus_tag="VN084_07660"
     CDS             563..2029
                     /locus_tag="VN084_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWT29374.1"
                     /translation="MSIARTLMVQGTTSDAGKSMLVAGICRVLHRKGIEVVPFKPQNM
                     ALNSAVTTDGGEIGRAQAVQAQACGLEPHSDMNPVLLKPNSDTGCQVIIHGRVVTDLE
                     AVGYHAYKPTAMRAVMESWRRLQAAYRCVIVEGAGSPAEINLREGDIANMGFAEAADC
                     PVILIADIDRGGVFAHIVGTLALLSESERARIVGFVINRFRGDIALLQPGLAWLERET
                     GKPVLGVLPYLHGLHIEAEDAVPLTPSPAKGEGALKVIVPVLPHIANHTDFDALRMHP
                     GVDFRYVGLHEAMPPADLVILPGSKNVRGDLQHLRDAGWQQALTRHLRYGGKVLGICG
                     GFQMLGEMLHDPLGIEGEAGSSQGLGLLQMETTLAATKRLARIAGKLIFADATIEGYE
                     IHMGVSNGEALARPLLALDGQPEGALSEDNRIAGTYLHGLFDHPASLAAWLQWAGQVT
                     ANGDFDYRAMREAELERLADCVERHLDWGTLAAWLPVT"
     gene            complement(2022..>2155)
                     /locus_tag="VN084_07665"
     CDS             complement(2022..>2155)
                     /locus_tag="VN084_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWT29375.1"
                     /translation="MGIVPLGEINRQFQDEQGRLNQAVAAIATKVIFVAAGLPLKLK"
BASE COUNT          393 a          648 c          700 g          414 t
ORIGIN      
        1 ttgaatcttt atcttgtcag gcacaccacg ccggacatcg cccctggcat ctgctatggc
       61 cagagcgatc tcgatgtggc cgagagctat gcccatgagt tggaagtggt gcgcagcaag
      121 ctcgccgccg tggagccggt cgccatcttc agcagcccgc tcaagcgttg cagccagttg
      181 gccgaggcca tcgccagcgt cacgtgctgc gatgtgcagc atgatcgacg cctcatggag
      241 ctccacttcg gcgattggga gttgctggcc tggaacgata tcccgcgtgg actggtggat
      301 gtctgggccg atgaccacgt gatgcaggcg cctccgtcgg gcgaatcctt ccatgcgctt
      361 tcactgcggg ttcaggattt cctgcaggaa gtgagcgcta accatctagg ccagaacctg
      421 gtcgtcatga cgcatgccgg cgtcatccgg gctttgcttg cccatgccct gaacatgaag
      481 ctcgccgaca gtttccggct gcagatcgat tacggcagca tcacgcagat cacggtggag
      541 aacggcatca cgcgcgtcgc ttatgtcaat cgctaggacg ctgatggtgc aaggcaccac
      601 atccgatgcg ggcaagagca tgctggtggc cggcatctgc cgcgtgctgc atcgcaaggg
      661 catcgaggtc gtaccgttca agccacagaa catggcgctc aattcggcgg tgaccacgga
      721 tggcggcgag atcggccgcg cccaggcggt gcaggcgcag gcctgcggtc ttgaaccgca
      781 cagcgacatg aacccggtgt tgctcaagcc gaattcggat accggctgtc aggtcatcat
      841 ccatggtcgg gtcgtcaccg atcttgaagc cgtcggttac cacgcctata aaccgaccgc
      901 catgcgtgcc gtgatggaat catggcgcag gttgcaggcg gcctatcgat gcgtcatcgt
      961 cgaaggcgcg ggcagccccg ccgagatcaa tctgcgcgaa ggcgatatcg ccaacatggg
     1021 cttcgccgaa gcggcggatt gcccggtgat cctcatcgcg gatattgatc gaggcggcgt
     1081 gttcgcgcat atcgtcggta cgctggcttt gctatcggag agcgagcgcg cccgcatcgt
     1141 gggcttcgtc atcaaccgtt tccgcggtga tatcgccttg ctgcaacccg ggctggcgtg
     1201 gctggaacgt gagacaggca agccggtgct gggtgtattg ccttacctgc acggcctgca
     1261 tatcgaagcc gaggatgccg tgccgttgac gccgtcccct gcaaagggcg agggggcgct
     1321 gaaagtcatc gtgccggtgc tgccgcatat cgccaaccat accgatttcg acgccttgcg
     1381 catgcaccct ggcgtggatt tccgctatgt cggcctgcat gaggccatgc cgccggcaga
     1441 tctcgtcatc ctgcccggga gcaagaacgt gcgcggcgat cttcagcatc tgcgagatgc
     1501 tggctggcag caagcgctca cacggcacct gcgttatggc ggcaaggtcc tcgggatctg
     1561 cggcggattc cagatgctgg gcgagatgct gcacgacccg ctgggcatcg aaggcgaagc
     1621 ggggtcgagt cagggcctag ggctgctaca gatggaaacg acactggccg cgactaaacg
     1681 gctcgcgcgg atcgcaggca aactgatctt cgcggatgcg acgatcgaag gttacgagat
     1741 ccacatgggt gtcagcaatg gcgaggcgct ggcaagaccc ttgctggcac tggacggcca
     1801 gccggaaggc gcgctgtcag aggataatcg catcgccggg acctacctgc atggcttgtt
     1861 cgaccatcct gcatcgcttg ctgcgtggct gcaatgggct gggcaggtca cggcgaatgg
     1921 ggatttcgat taccgcgcca tgcgcgaagc cgaactggaa cggctggccg attgcgtcga
     1981 gcggcatctc gactggggca ccctcgcggc ctggctgcct gtcacttgag cttgagcggc
     2041 aagcccgcgg cgacgaagat gactttcgtt gcgatcgcgg ccaccgcctg gttcaggcgc
     2101 ccttgttcat cctggaactg acggttgatc tcgccaagcg gcacgatgcc catgc
//
LOCUS       DATMTW010000391         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_166354, whole genome shotgun sequence.
ACCESSION   DATMTW010000391 DATMTW010000000
VERSION     DATMTW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166354"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VN084_07670"
     CDS             <1..811
                     /locus_tag="VN084_07670"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWT29376.1"
                     /translation="RLPIPEPPASSIAAPVAADTPAQPTPTVASEPPVQDTDADTPAG
                     DPKPAPATFIESEYELRRQVNPGAEPGKIGVAHVSYKTDATGRYSIKSVAEATGIVSL
                     FVSGRLRQESEGRITENGLQPERFLYQYGDNEAKAQHAEFDWAGGVLQLRSAKGKSSV
                     RLVPGAQDLLSFMYQFMFTPPLEEMTLNVTNGKRLSTYNYSFEGEEQIQTGLGELKTM
                     HISKSASDGKDRTDVWLAMDYQYIPVKIRKTEDNGSVIEEVVTRLSTDLMK"
     gene            817..>1651
                     /locus_tag="VN084_07675"
     CDS             817..>1651
                     /locus_tag="VN084_07675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWT29377.1"
                     /translation="MNQNLLRLAGMAMADVLTASGPADAKLGAFFRGHRELGTKDRAF
                     IAESVYGVLRRKRWLAHLADGEDPRKLLVAWLVRVQGSSLRDLDEVLNKQQKEWAQEI
                     KARSVDGLSLAEQVDLPDWLWERLLAQYGESEALAIARSMHQSAALDLRVNTVKTNRA
                     DVLARFAAESAQSGFTAQATPYAPNGIRLPQRMGINRHVLFTEGRVEVQDEGSQLLSH
                     LLAPKRGDMVADFCAGAGGKSLAIGALMRNTGRIYAFDVSEKRLHNLGQRLKRSGLSN
                     LH"
BASE COUNT          347 a          511 c          514 g          279 t
ORIGIN      
        1 gcgactcccc atcccggagc cgccggcgtc ctcgattgcc gcgccggttg cggcagatac
       61 acccgcgcaa ccgacaccca ccgtcgccag cgagccccct gtgcaggaca ccgacgcgga
      121 cacgcctgct ggggacccga agccagcgcc cgcgactttc atcgagtccg agtatgagtt
      181 gcggcgacag gtgaatccgg gcgcggaacc aggcaagatc ggcgtcgcgc atgtcagcta
      241 caagactgac gcgaccggcc gctacagcat caagagtgtc gccgaggcga ccggcatcgt
      301 ctccctgttc gtctcgggac gtttgcggca ggagagtgaa gggcgtatca ctgagaatgg
      361 cttgcagccg gagcgtttcc tctaccagta cggcgataac gaagccaagg cccagcatgc
      421 cgaattcgac tgggccgggg gagtgttgca gctgcgatcc gccaagggca aaagcagcgt
      481 gcggttggtc cccggcgcac aggatctgct gagtttcatg taccagttca tgttcacgcc
      541 gccgctggaa gagatgacgc tgaacgtgac caacggcaag cgcctcagca cgtacaatta
      601 cagctttgaa ggcgaagagc agatccaaac cgggctgggc gagctcaaga ccatgcacat
      661 ctccaagagc gccagcgatg gcaaggatag aacggatgtg tggctggcga tggattatca
      721 gtacatcccg gttaaaatcc gcaagaccga agacaatggc agcgtgatcg aagaggtcgt
      781 cacccgcctt tccaccgacc tcatgaaatg aatggtatga atcagaacct gttaagactg
      841 gccggcatgg ccatggccga tgtactcacc gcgagcggcc ctgccgacgc gaaactcggt
      901 gccttcttcc gcggccatcg cgagcttggc accaaggatc gcgccttcat cgccgaatcc
      961 gtctacgggg tgctgcgccg caaacgctgg ctggcgcatc tggccgatgg cgaagacccg
     1021 cgcaagttgc tggtcgcctg gctagtgcgc gtacagggca gtagcctgcg tgatctcgat
     1081 gaggtcctga acaaacagca gaaggaatgg gcgcaggaga tcaaggccag atcggtcgac
     1141 ggcctcagcc tggcagagca ggtcgacctg ccggactggc tatgggaacg tctactggcc
     1201 caatacggcg agtctgaagc gctggccatc gcccgtagca tgcatcagtc ggctgcgctc
     1261 gatctgcgcg tcaataccgt caagaccaac cgcgcggacg tacttgcccg tttcgccgcc
     1321 gagagtgcgc aatccggctt caccgcgcag gcgacgccat atgcgcccaa cggcatccga
     1381 ctgccgcagc gcatgggcat caaccgccat gtgctgttca cagaaggccg ggtcgaagtg
     1441 caggatgaag gtagccagtt attgagtcac ctgcttgcgc ccaagcgcgg cgacatggtg
     1501 gcggatttct gcgccggcgc cggcggcaag agtctggcca tcggcgcgct gatgcgcaac
     1561 accggtcgca tctatgcttt tgacgtctcg gaaaagcgcc tgcataacct gggtcagcgc
     1621 ctcaagcgtt cgggtctttc caatctgcac a
//
LOCUS       DATMTW010000392         5593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_166748, whole genome shotgun sequence.
ACCESSION   DATMTW010000392 DATMTW010000000
VERSION     DATMTW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166748"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VN084_07680"
     CDS             <1..1722
                     /locus_tag="VN084_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWT29378.1"
                     /translation="LGSMPGLYISNDRSWEYVGVRGFSRPGDYNSRVLLLVDGMRMND
                     NIYNQAYIGNDFLLDLDLVQRVEFSPGPGASIYGDNAFLGVINVVTKKAGDMKGVATS
                     AELSRYRGYKGSVRYGGQWDNGAELMLSATASDTAGRDWRYAEFAGKAEGLDGEDLSK
                     VFAKFSFEGLTLEAGRMRRDKNNPTAPFGTTFNDPRYETRDTHTFASATYTQALSEQT
                     EMRLHFSHGRFDYEGHYPAGVLNIDRTHGARNIMEARLTSSVFERHKLVTGIEYIRDD
                     RLDMVNFDVAPAQTYLRERRDAEKFGIYLQDEIRLHERWLLNAGLRYDHYDSFGDTAN
                     PRLALIHQPTPYDTVKLIYGRAFRAPNAYELYYGSTTMGALQKGNPDLRPEIMQSYEL
                     TWDTALASAWHAKAGLFQYRIRDTIGQVLDPVDHLWVFRNLGRVRTNGAELGLDGLLL
                     NGIRLKSSLTYQDAREIETDEWLVNSPRILAKLGLSGRFYGDWQAGAEIQHIGRRRTM
                     ADETGGATLANLTVSRRDYLPGLDVSLGFYNLFDKRYAVPADLINVQDSLRQDGRMFR
                     LKLDYRF"
     gene            1723..2253
                     /locus_tag="VN084_07685"
     CDS             1723..2253
                     /locus_tag="VN084_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HWT29379.1"
                     /translation="MLRPRLRRLLLLLLFWATLPAMAEPVHEYELKTAFLYNFILFTQ
                     WPADSTLQNSPYFNVCTIGPDPFGPVLEQLQGRKIGNKRLFLRREIGLDEARDCHLLY
                     ISANDPENLARIREQLQDASVLTVSDLNPASSERTIINMSIDNNRLVFDIDMMAAKQA
                     RLVMSSKLLRLARRVY"
     gene            2260..4137
                     /locus_tag="VN084_07690"
     CDS             2260..4137
                     /locus_tag="VN084_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWT29380.1"
                     /translation="MNVSTSPQAVAPALSKLHLLALGLALLMASLCLILVEYASLRSS
                     LAAGVEVQMRIVAGNSVGALMFKDNAAADEILSSLAASSDIDAAAIYDGSDARFAEYT
                     RARALPDIHAPATASDTTPTSSLQHTDFWQPVTYAGNRIGMLYVRANLSKLYRQLAWY
                     IATTSMVMLTTLFAAAVLLGRLQRAVVHAEERVGYLVNYDTVTHLPNRYAFNQRFQAL
                     LDEAKATQSRLALLVLDLDDFKLINDTLGHHVGDSLLRVVAQRLMSVVRHEDVIYRVG
                     GDEFAMILSHSEDVERVEVIAQKFIHALSEPLVVDERNFYIGASIGASLFPEDGNDVA
                     HLLRDADAAMYHAKSRGKNNFQMFSEEMHHKSSTRLNLESELRIAMEQQQFELHYQPQ
                     VDLASRKLVGAEALIRWRHPTRGLLLPNDFIPVAEETGLIGSIGNWVLRTACQQAKSW
                     QEQRFRPIRVSVNLSGRQFREDTLVESITTILKETEMNPDMLELEITESVLMDDAETT
                     VSRLALLRAMGMHLAIDDFGTGYSSMAYLKRFPVERLKIDRSFIRDIPQDADDVAITV
                     ATIQMAHSLKLEVVAEGIETLAQTTFLSEQNCDVGQGYLFSQALDQQQMTDLLRSGKT
                     L"
     gene            complement(4359..4964)
                     /locus_tag="VN084_07695"
     CDS             complement(4359..4964)
                     /locus_tag="VN084_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWT29381.1"
                     /translation="MSLNQQLRKTHPMVLVAAVALTIFSLLGSAAITGLLPTAHSDNA
                     APTESIGQADAAESGHATGVDQFYSKARPANGNAQPSVAMNAPPAAACADCGRIVSIR
                     AIEQEGEASGLGAVAGGVAGGVIGNQIGKGRGNTLMTVLGVGGGAYAGHEIEKNMKTK
                     TAYVIRIEMQDGSIRKVTQYNEPQFAVGDRVRLNRGQLVNA"
     gene            complement(5072..>5593)
                     /locus_tag="VN084_07700"
     CDS             complement(5072..>5593)
                     /locus_tag="VN084_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWT29382.1"
                     /translation="IMNTKANKHVMYVFALASIVLMPCLSAPAVAGTADNLNQSPLET
                     EFRKLDLNHDDRLSSEEVARDKDFAGNFARADTNGDGVLIADEYGNFKSAAQQKRLEL
                     FLDDSAVTARVKAELVKDSGVKGLNISVETYKGEVILSGFVENEAQVRRVVEIASGVR
                     GVQTVKNGLSVRG"
BASE COUNT         1108 a         1714 c         1631 g         1140 t
ORIGIN      
        1 ctcggcagca tgcccgggct ctatatcagc aacgaccgca gctgggagta tgtgggtgta
       61 cgcgggttct cgcgccccgg ggattacaac tcgcgtgtgc tactgctggt cgacggcatg
      121 cgcatgaacg acaacatcta caaccaggcg tatatcggca acgatttcct gctcgatctc
      181 gatctggtgc agcgtgtcga attctcaccg gggccgggcg cttcgatcta tggcgacaat
      241 gcctttctcg gcgtgatcaa tgtcgtcacc aaaaaggccg gcgacatgaa aggcgtggca
      301 accagcgccg agctgagccg gtatcgcggt tacaagggta gcgtgcgtta tggtggccaa
      361 tgggataacg gcgccgagct gatgctttcg gctaccgctt cggacacggc cggtcgcgac
      421 tggcgctatg ccgaattcgc gggcaaggca gaaggcctcg atggcgagga tctgagcaag
      481 gtgttcgcca agttctcgtt cgaaggcctg acgctggaag cagggcgcat gcggcgcgac
      541 aagaacaacc cgaccgcacc gttcggcacg actttcaacg atccgcgtta cgagacgcgt
      601 gacacccata ccttcgccag cgcgacctat acgcaggcgc tatcggaaca gaccgagatg
      661 cgcctgcatt tcagccacgg gcgcttcgat tatgagggcc attatcctgc cggcgtgctg
      721 aacatcgatc gcacccacgg tgcgcgcaac atcatggagg cgcgtctcac cagcagcgtc
      781 ttcgagcgcc acaagctcgt cactggcatc gaatatatcc gggacgatcg gctggacatg
      841 gtcaatttcg atgtcgctcc cgcgcagacc tatctgcgcg aacgccgcga cgccgaaaaa
      901 ttcggcatct atctgcagga cgagatccgc ttgcacgagc gatggctgct caatgccggc
      961 ttgcgttacg atcactacga cagtttcggc gataccgcca accccagact ggccttgatc
     1021 caccaaccga cgccgtacga taccgtcaag ctgatctacg gccgtgcctt ccgcgccccc
     1081 aacgcctacg agctgtatta cggcagcacg accatgggcg cgttgcagaa gggtaatccg
     1141 gatctgcgcc cggagatcat gcaaagctac gagttgacct gggacacggc acttgccagc
     1201 gcctggcatg cgaaggccgg gctgttccag taccgtatca gggacaccat cggccaggtg
     1261 ctcgacccgg tcgatcatct ctgggtgttc cgaaatctcg gtcgcgtacg gaccaatggc
     1321 gctgaactcg gcctcgacgg gctgttgctg aacggtatcc gcctgaagtc cagcctcacc
     1381 tatcaggatg cgcgcgagat cgaaaccgac gagtggctgg tcaattcgcc ccggatactg
     1441 gccaagctcg ggctatccgg ccgcttttac ggggactggc aggctggcgc ggagatccag
     1501 catatcggca ggcggcgcac catggcggac gaaacgggcg gcgccacgct ggccaatcta
     1561 accgtgagcc ggcgcgacta tctgccgggc ctcgacgtgt cgctagggtt ctataacctg
     1621 ttcgacaagc gttatgccgt cccggcggac ctcatcaacg tgcaggacag cctgcgccag
     1681 gatgggcgca tgttccgtct gaagctggac tacaggttct agatgctgcg cccccgctta
     1741 agacgcttgc tgcttttgtt gctgttctgg gcgaccctgc cagcgatggc tgagccggtg
     1801 cacgagtacg aactgaagac ggcttttctc tataacttca tcctgttcac gcaatggccg
     1861 gccgacagca cgctgcaaaa cagcccgtat ttcaatgtct gtaccatcgg cccggatccg
     1921 ttcggccctg tgctggagca gctgcagggg cggaagatcg gcaacaagcg cttgttcctg
     1981 cggcgtgaga tcggccttga tgaggcgcga gattgccatc tgctgtatat cagtgccaat
     2041 gatccggaaa atttggctag aatacgggaa cagctgcaag acgccagcgt gctgaccgtc
     2101 agcgatttga accccgcttc ttctgagaga accatcatca atatgagcat tgacaacaat
     2161 cgtctggtat tcgatatcga tatgatggct gccaagcagg ccaggctcgt gatgagttcc
     2221 aaactgctac gtcttgcccg gcgcgtctac tagctccaga tgaacgtcag cacctcgccc
     2281 caggcggtcg cgccagcctt gagcaagctg catctgctgg cgttgggcct ggcgctgctc
     2341 atggccagcc tctgcctcat tctggtcgaa tatgcctctc tgcgcagcag cctcgccgcc
     2401 ggggtcgagg tgcagatgcg catcgtcgcg ggtaacagtg tcggcgcgct gatgttcaag
     2461 gacaacgcgg ccgcagacga gatcctgtcc agtcttgccg cttcttccga tatcgacgct
     2521 gccgccatct acgatggcag cgatgcgcgt ttcgccgaat acacccgtgc tcgggcgctg
     2581 ccggatatcc atgcgccggc caccgcctcc gacaccaccc cgacctccag cttgcagcac
     2641 accgacttct ggcagcccgt gacctatgcc ggcaatcgca tcggcatgct ctatgtgcgg
     2701 gcgaacctca gcaagctcta tcgccagctc gcctggtata tcgcgaccac cagcatggtc
     2761 atgctcacca ccttgtttgc cgcggctgtc ctgctcggtc ggctgcagcg cgctgtcgtc
     2821 catgccgaag agcgtgtggg ttacctcgtc aattacgaca cggtgaccca tctaccgaac
     2881 cgctatgcgt tcaatcagcg cttccaggcg ttgctcgacg aggccaaggc gacgcagagc
     2941 cggcttgcct tgctcgtgct cgatctcgat gacttcaagc tcatcaacga tacgctcgga
     3001 catcatgtcg gtgacagcct gctgcgcgtg gtggcgcaac ggttgatgtc cgtggtgcgg
     3061 catgaagacg tcatctatcg cgtcggcggc gacgagttcg ccatgatcct gagccatagc
     3121 gaggatgtcg agcgtgtcga ggtcatcgcc cagaagttca tccatgcgct gtccgaacct
     3181 ttggtggtgg acgaacgcaa tttctacatc ggcgccagta tcggcgccag tctctttcct
     3241 gaagacggca acgatgtcgc ccatctgctg cgggatgccg atgccgccat gtatcacgcc
     3301 aagagccgcg gcaagaacaa tttccagatg ttctctgaag agatgcatca caagagctcc
     3361 acacgcctta accttgagag cgaactgcgt atcgcgatgg aacagcagca gttcgagctg
     3421 cattaccagc cccaggtcga cctcgccagc cgcaaactgg tcggtgccga agccttgatc
     3481 cgctggcggc atcccacacg tggcttgctg ttgcctaacg atttcatccc ggtcgcggaa
     3541 gagacgggct tgatcggcag tatcggcaac tgggtcctgc ggaccgcctg tcagcaggcg
     3601 aagtcctggc aggaacagcg cttcaggccg atacgcgtca gcgtcaacct gtccggacgc
     3661 cagttccgtg aagatacgct ggtcgaatcc atcaccacga tcctcaagga aacggagatg
     3721 aatcccgaca tgctcgagct ggagatcacg gaaagcgtgc tgatggacga cgccgagacc
     3781 acggtctcgc gcttggcgct gctgcgtgcc atgggcatgc atctggccat cgacgatttt
     3841 ggtacgggct attcttccat ggcttacctc aagcgcttcc cggtcgagcg gctgaagatc
     3901 gaccgcagtt tcatccgtga tatcccgcaa gatgcagacg atgtggcgat cacggtcgcg
     3961 accatccaga tggcacacag tctcaagctg gaggtggtcg cggaaggcat cgagaccctc
     4021 gcgcagacga cgttcctgtc tgagcaaaac tgcgacgtgg ggcagggcta tctattcagc
     4081 caggctctcg atcagcaaca gatgacggat cttctgcgtt ccggcaagac cctctgaaca
     4141 cgccccacgc tgtattcacc tcatgggctt ccatgccatc gctggtatga ggctacgtcc
     4201 agtcatgcgt ggcacacaag ccatgggcct cgcatggacc atcggaaaca cacgaaccat
     4261 cggacacaca caggaatagc aggcaaaaaa aagcgaacct tctcggggag atgaaggccc
     4321 gctatcagga gaacaaccta atttacgaca gggaagcgct aagcgttcac cagttggccg
     4381 cgattcagtc ggacgcgatc gccaaccgca aactgaggtt cgttgtattg tgtgaccttg
     4441 cggatactgc cgtcctgcat ctcgatgcgg atgacatagg cggtcttggt cttcatgttc
     4501 ttttcgatct catggcccgc ataggccccg ccaccgacgc cgagtacggt catcagggtg
     4561 ttgccgcgac ctttgccgat ctggttgccg atgacgccgc ctgccacacc gccagcgaca
     4621 gcgccgagac cggaggcctc gccttcctgc tcaatggcgc ggatcgatac gatcctgccg
     4681 caatcggcac aggctgctgc gggcggcgca ttcatggcca cgctgggttg tgcattgcca
     4741 ttggccggcc gggccttgct gtagaactga tcgaccccgg tcgcatgccc ggattcggct
     4801 gcgtcagcct ggccgatgct ttccgtcggc gcggcattgt cggagtgtgc cgtcggcaac
     4861 aggccggtga tcgcggcact gccgagcaga ctgaagatgg tcagcgccac ggcggcgacc
     4921 aggaccatcg ggtgggtctt gcgtaattgt tgattcaaag acatcgcttc tctcccatca
     4981 tgcatcatgt tgttgatctg ttggtattcc tgatgttgat gatgcacggt agcgctggat
     5041 caggcttgac ctgacgcacc gcgccgcagg gttagccccg caccgacaaa ccgttcttga
     5101 cggtctgcac accgcgcacg ccggaagcga tctccactac gcgacgcact tgtgcctcgt
     5161 tttcgacgaa tccgctcagg atcacctcac ccttgtaggt ttcgacgctg atgttcagac
     5221 ccttgacgcc gctgtccttg accagttcag ccttgacgcg cgcagtcacg gcactgtcgt
     5281 ccaggaagag ttccaggcgc ttctgttgtg cggcactctt gaaattgccg tactcatcgg
     5341 cgatcagcac gccgtcgccg ttggtgtcgg cgcgtgcgaa gttacccgcg aaatccttgt
     5401 cgcgagcgac ttcctcactc gacagacgat catcgtggtt gaggtccagt ttcctgaatt
     5461 ccgtctcaag tggcgattga ttcaggttgt ctgcggtgcc tgcaacggca ggtgcggaca
     5521 agcaaggcat cagcacgatg ctggcaaggg caaagacgta catcacatgt ttattggctt
     5581 tggtattcat gat
//
LOCUS       DATMTW010000393         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_301297, whole genome shotgun sequence.
ACCESSION   DATMTW010000393 DATMTW010000000
VERSION     DATMTW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301297"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1038)
                     /gene="fnr"
                     /locus_tag="VN084_07705"
     CDS             complement(139..1038)
                     /gene="fnr"
                     /locus_tag="VN084_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HWT29383.1"
                     /translation="MPATLPAMGAAFLDVNQISRTCSRRQSVHTPTAGYDMHDVIAIH
                     DDSNAVCLSDDQPSCRNMTPETIKVACSNCNLRELCMPIGFNPNDLQRLDEVVATRRR
                     IKQGEHLFSNGDPFTSLYAIRIGFFKTSISSEDGREQVTGFQMAGEIIGLDGIVSDRH
                     SCNAIALEDAEVCVMPFANVEELSREFPVLQRHVHKIMSREIVRENGVMMLLGNMRAE
                     ERLAAFLLNLVQRLHARGFSQSEMVLRMTREEIGSYLGLKLETVSRTFSKFQEEGLLE
                     VKQRQIRIVDQAGLQKLVNGAAC"
     gene            1077..2480
                     /gene="hemN"
                     /locus_tag="VN084_07710"
     CDS             1077..2480
                     /gene="hemN"
                     /locus_tag="VN084_07710"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HWT29384.1"
                     /translation="MNTEILKQPVTTLPGALLTRFDIPGPRYTSYPTADRFVEAFNED
                     AYRQALEQRRVGGLALPLSLYVHIPFCESLCFYCACNKIVTKRHERSREYLRYLGREV
                     DLHVAQLGSGQPISQLHLGGGSPTFFNDDELDELMTMLRRGFVFDPAGEYSIEVDPRT
                     VDADRLAHLHALGFNRLSFGVQDFDEEVQKAVHRIQPAEQVFELVQEARRLGFQSINA
                     DLIYGLPKQTPESFERTLQTMTKLRPDRIALYAYAHLPERFKPQRRIHAAELPLAGAK
                     VLMLSEAIAKFISAGYVYIGMDHFALPDDSLAVAKRQGRLHRNFQGYSTCSDCDLIGL
                     GVSAIGRIGATYSQNAKTLEEYYDKLDRGSFPVVRGLALSRDDLVRRAVIMALMCQGR
                     VQFESIELAYMVDFRRYFAVELRALAELAESGIIEIEEGAIQVTSTGWFFVRAIAMLF
                     DRYLQLDRNRARFSRII"
     gene            2490..3164
                     /locus_tag="VN084_07715"
     CDS             2490..3164
                     /locus_tag="VN084_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWT29385.1"
                     /translation="MALQTSLALTALFMGLAGGPHCVAMCGPACGALKSTPESPFLPL
                     LFHAGRLIGYASLGAVAAASVGGLAWFSDQTLALRPLWTFFHVAILCWGIVLLATARQ
                     PQWADRYGRAIWAWARKGTSTSTGALASGISWALMPCGLLYSALMVAALNAHALGGAV
                     SMAAFAFGTTVSLLLAPWLWLRIRQGRLGFNQATGIRIAGLLLTAAAGWAIWMDLHHG
                     IKVWCS"
     gene            complement(3265..>3921)
                     /locus_tag="VN084_07720"
     CDS             complement(3265..>3921)
                     /locus_tag="VN084_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWT29386.1"
                     /translation="EFNFSQQPVFPLASEDSEGRVIYIGNLSKILAPELRVSYVAATS
                     SIVDRLAREVALFDQHNNNAIEMTIAELMRSGEIKRHSLKMTKVYQDRRDHFQQLIDD
                     ELWQWVDYQSPQSGLAFWLRFKEAVDIKRMAKKSKEEKIHFAFGKTYSAAGEEVPAMR
                     LGYAHMNEKEMQQGFQRLKHIVVSCLVATIISCCSATELIEMSPMSSGLVKMSHTLED
                     "
BASE COUNT          678 a         1178 c         1230 g          835 t
ORIGIN      
        1 gacgcccccg ggcggcaccc ggcccccgat gcgcggcgtc cacgaacact gaatccgcaa
       61 aacgcaagcg caaaaaagcc gatggcgcct tcaaggcgcc atcggctttt ctgaggaccc
      121 gttctcgaaa attccgggtc agcaggccgc gccgttgacc agtttctgca ggccggcctg
      181 gtccacgatg cggatctggc gctgcttgac ctccagcagg ccttcctcct ggaacttcga
      241 gaaggtgcgg ctgaccgtct ccagcttgag gcccaggtag ctgccgatct cttcgcgcgt
      301 catgcgcagc accatctcgg actgtgagaa gccgcgggca tgcaagcgct gcaccaggtt
      361 gagcaggaag gcagccagac gttcttcggc gcgcatgttg ccgagcagca tcatgacgcc
      421 gttctcgcgc acgatctcgc ggctcatgat cttgtgcaca tggcgctgca gcacggggaa
      481 ttcgcgggac agctcctcga cattggcgaa cggcatgacg cagacctcgg cgtcttccaa
      541 tgcgatggcg ttacagctgt ggcgatcgct gacgatgcca tcgagcccga tgatctcgcc
      601 ggccatctgg aagccggtga cctgctcgcg gccatcctcg ctggagatcg aggtcttgaa
      661 gaaaccgatg cggatggcat agagcgatgt gaagggatcg ccgttgctga acaggtgctc
      721 gccctgcttg atccggcggc gcgtggccac cacttcgtcg agccgttgca ggtcgttcgg
      781 gttgaagccg atcggcatgc acagctcacg caagttgcag ttggaacagg cgaccttgat
      841 ggtttccggt gtcatgtttc tgcaggaggg ttgatcgtcg ctcaagcata ctgcgtttga
      901 atcatcgtgg atagcgatta catcgtgcat gtcgtagcct gctgtaggtg tatgaacgga
      961 ttgtcgccgg gaacatgttc ggctgatttg atttacatca aggaaggccg cgcccattgc
     1021 aggcaaagtg gctggcattc gcgagcactg gctcgattct ttcaagagac tgtccgatga
     1081 ataccgagat cctgaaacag ccggtgacga cgctgcccgg cgcgctgctg acccgcttcg
     1141 atatccccgg gccacgctat acctcgtatc cgacggctga ccgctttgtc gaagccttca
     1201 acgaggatgc ctaccgacag gcgcttgaac aacgtcgcgt cggcggactg gccttgcccc
     1261 tgtcgctcta tgtgcatatc ccgttctgtg aatcgctgtg cttctactgc gcctgcaaca
     1321 agatcgtcac caagcgtcac gaacgtagcc gcgaatacct gcgctacctt ggtcgggagg
     1381 tcgatctgca tgtggcgcag ctaggcagcg ggcaacccat ctcgcaactg catctgggtg
     1441 gcggttcgcc cacctttttc aatgacgacg agctggacga gctgatgacc atgctgcgcc
     1501 gcggctttgt atttgatccg gccggggaat attcgatcga ggtcgatccg cgtacggtcg
     1561 atgccgatcg cctggcgcac ctgcatgcgc tgggcttcaa tcgcctgagt ttcggcgtgc
     1621 aggatttcga cgaagaggtg cagaaggcag tgcatcgcat ccagcctgcc gaacaggtgt
     1681 tcgagctggt gcaggaagcg aggcggctgg ggttccaatc catcaatgcc gacctgatct
     1741 atggcctgcc gaagcagacg ccggagtcct tcgagcgcac cttgcagacc atgacgaagc
     1801 tgcgccctga ccggatcgcg ctctacgcct atgcccatct gccggaacgc ttcaagccac
     1861 agcgtcgcat ccatgccgcc gagctgccgc tggcgggtgc caaggtgctg atgctgtccg
     1921 aagccatcgc caagttcatc agtgccggtt atgtctacat cggcatggac catttcgcgc
     1981 tgcccgacga tagcctggcc gtggccaagc gccagggacg gttgcaccgt aacttccagg
     2041 gctacagcac ctgttccgat tgtgatctga tcggcctcgg cgtctcagcc atcggccgca
     2101 tcggcgccac ctacagccag aacgccaaga ccctggaaga gtattacgac aagctcgatc
     2161 gcggcagctt tccggtggtg cgcggcctgg cattgtcgcg cgatgatctc gtgcggcgcg
     2221 ccgtgatcat ggcgctgatg tgccagggtc gggtgcagtt cgaatccatc gagcttgcct
     2281 acatggtgga tttcaggcgc tatttcgccg tcgagttgag ggcgctggcg gagcttgcgg
     2341 aaagcggcat catcgagatc gaggagggcg ccattcaggt gacgagcact ggctggttct
     2401 tcgtccgtgc catcgccatg ctctttgatc gctatcttca gctcgaccgc aaccgcgcgc
     2461 gtttttcgcg catcatctag acgacgacca tggccttgca gacttcgctg gcgttgactg
     2521 cgctgttcat gggcctggcc ggcgggccgc attgcgtcgc catgtgcggg cctgcctgcg
     2581 gcgcactgaa gtcgacgccg gaatcgcctt tcctgccgtt gctattccat gccgggcggc
     2641 tcatcggcta tgcctcgcta ggggcggtgg cagccgcctc cgtgggcggg ctggcatggt
     2701 tcagcgacca gacgctggct ttacggccct tgtggacctt cttccatgtg gcgatcctgt
     2761 gctggggcat cgtgctgttg gcgacggcgc gacagccgca atgggctgac cgctacgggc
     2821 gcgcgatctg ggcctgggca cgcaagggta cttcgacctc gaccggcgca ctcgccagtg
     2881 gcatcagctg ggccttgatg ccttgcggcc tgttgtattc ggcactgatg gtcgccgcgc
     2941 tgaatgccca tgcgctgggt ggcgccgtca gcatggcggc attcgcgttc ggcaccacgg
     3001 tctcgctact gcttgcgccc tggctctggc tgcggatccg gcagggccgg ttgggcttca
     3061 accaggcgac cgggatccgt atcgccgggc tgctactgac ggcggcggcc ggctgggcga
     3121 tctggatgga tctgcatcac ggcatcaagg tctggtgtag ttgaacgtgg atgcgcacgc
     3181 gagtgcgtga ctgcagcatc aggccaggat ggcttttggg cagtcgagtg ggtgcctgga
     3241 gtcgcggcac ccagtcctcg tggctcagtc ttcgagggtg tgcgacatct tgaccaggcc
     3301 gctcgacatc gggctcatct cgatcagttc ggttgccgag caacaactga tgatggtggc
     3361 gaccaggcag ctgaccacga tatgtttcag gcgctgaaaa ccctgctgca tctctttctc
     3421 gttcatgtgg gcatagccca gtcgcatggc cggcacttct tcgccagcgg ccgagtaggt
     3481 cttgccgaag gcgaaatgga tcttttcttc cttcgatttc ttggccatgc gcttgatatc
     3541 cacggcttcc ttgaaacgta gccagaaagc caggccgctt tgcggcgact ggtaatccac
     3601 ccattgccag agctcatcgt cgatgagctg ctggaaatgg tcgcgcctat cctggtagac
     3661 cttggtcatc ttgaggctat gacgtttgat ctcgcctgag cgcatgagct cggcgatggt
     3721 catctcgatg gcgttattgt tgtgctggtc gaacaacgcg acctcgcgcg cgagtcgatc
     3781 gacgatggac gatgtggccg ccacatagct gacccggagt tccggcgcca ggatcttcga
     3841 caggttaccg atatagatga cgcgcccctc gctgtcttcg ctggcgagcg ggaaaacggg
     3901 ctgctgcgaa aagttgaatt c
//
LOCUS       DATMTW010000394         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate SMAG_U13023
            k141_301626, whole genome shotgun sequence.
ACCESSION   DATMTW010000394 DATMTW010000000
VERSION     DATMTW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943623
            Sequence Read Archive: SRR7457707
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (soil metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:27:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,547
            CDSs (total)                      :: 1,535
            Genes (coding)                    :: 1,525
            CDSs (with protein)               :: 1,525
            Genes (RNA)                       :: 12
            tRNAs                             :: 12
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301626"
                     /isolate="SMAG_U13023"
                     /isolation_source="soil"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..651)
                     /locus_tag="VN084_07725"
     CDS             complement(58..651)
                     /locus_tag="VN084_07725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HWT29387.1"
                     /translation="MTTTLRDLVGLGHQAPSLKDSALIMIDCQNTYRRGIMQLEGVEP
                     AIREAQKLLEMARDLKVPVIHIQHDAGEGSPYDVRADIGMISSEVSPISGESVITKNY
                     PHAFIQTEVDQRLKALGVQNIVLAGFMTHMCINSTAHGGFNLGYAPIVVASATATRAL
                     PASDGSVVPAAAVQAAALAATRDLYAVVVESAGDLVA"
     gene            complement(679..1053)
                     /locus_tag="VN084_07730"
     CDS             complement(679..1053)
                     /locus_tag="VN084_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HWT29388.1"
                     /translation="MHSPRIVATPAAEALLDELRAKHGELMFHQSGGCCEGSAPMCLT
                     AGEFLVGDSDVKIGYVHGVPFYMAKFQFQYWEHTHMTLDAIAGHGGQFSLEQGTGKHF
                     LIRSRLFTDEELADLAPVEPAP"
     gene            complement(1156..2676)
                     /gene="adh"
                     /locus_tag="VN084_07735"
     CDS             complement(1156..2676)
                     /gene="adh"
                     /locus_tag="VN084_07735"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWT29389.1"
                     /translation="MYLESLKNLTSKMPFKAKYDNYIGGKWVAPVDGQYFDNISPLTG
                     KPFCRVARSNEKDVNLALDAAHAAREAWGKTSTTTRSNILLKIADIMESNLELIATAE
                     TIDNGKPIRETMNADIPLAIDHFRYFAGAIRAQEGGISEIDHETMAYHFHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALAAGNVAVMKPAEQTPASILVVMELIEHLLPPGVLNIVNG
                     HGVEAGKPLATSPRIAKIAFTGSTSVGRLIMQYASQNLIPSTLELGGKSPNIFFEDIM
                     DADDAYFDKCLEGFTLFALNQGEVCTCPSRALIQESIYDKFMERALKRVKAIKHGNPL
                     DSNTMIGAQASSEQVEIIMSYINLGKEEGAQLLTGGVQKKLEGDLSEGYYIEPTVFKG
                     HNKMRIFQEEIFGPVLSVTTFKDEAEALAIANDTMYGLGAGVWSRDGARAFRMGRGIQ
                     AGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYSPNALGFF"
BASE COUNT          510 a          774 c          841 g          617 t
ORIGIN      
        1 ttcggggctt ttttattggg actgatcgcc aggttttttc gcttacctac cagaggccta
       61 ggccaccagg tcgccagctg attcgaccac caccgcataa agatcgcgcg tcgcagcgag
      121 tgccgctgcc tgcacggcag ccgccggtac cacgctgcca tcactggccg gtaatgcgcg
      181 cgtagccgtc gcactggcga cgacgatagg cgcgtagcca aggttgaagc cgccatgcgc
      241 cgtggagttg atgcacatgt gcgtcatgaa gccggcgagc acgatgttct gcacgcccag
      301 cgccttcaat cgctgatcga cctcggtctg gatgaaggcg tgcggatagt tcttggtgat
      361 gacggattca ccgctgatcg gcgacacttc ggacgagatc atgccgatat ctgcacgcac
      421 gtcatacggg ctgccttcgc ctgcatcgtg ctggatatgg atcacgggca ccttgaggtc
      481 gcgcgccatc tccagcagct tctgcgcctc gcggatcgcc ggttccacgc cttccagctg
      541 catgatgccg cgccgatagg tgttctggca gtcgatcatg atgagcgccg aatccttgag
      601 gcttggggcc tgatgcccaa ggccgacaag gtcacgtaat gtggtggtca ttgtgcttct
      661 cctgaattgg gttggtgact acggtgccgg ttcgacaggc gcgaggtcgg ccagttcttc
      721 atcagtgaac agtcgcgaac ggatcagaaa gtgcttgcca gtgccctgct ccagcgagaa
      781 ctgtccgcca tggccggcga tagcgtcgag cgtcatgtgc gtgtgctccc agtactggaa
      841 ctggaacttc gccatgtaga acggcacgcc gtggacatag ccgatcttga cgtcagaatc
      901 gccgacaagg aattcgccag ccgtcagaca catgggggcg ctgccttcgc aacatccgcc
      961 ggactggtgg aacatcagct cgccgtgttt cgccctgagt tcatcgagca atgcttcagc
     1021 cgccggtgtg gcgacgatgc ggggtgagtg cataaggagt cctctgttgg ttcgcttttc
     1081 aagtgtaacg caataaaaaa cgggtacgac cccgaacaga tgtcgtaccc gttgcgtgct
     1141 aactgcagtc gacgcttaga agaaaccgag cgcgttcggg ctgtagctca ccagcaggtt
     1201 cttggtttgc tggtagtgat ccagcatcat cttgtggttc tcgcggccga taccggattg
     1261 cttgtaaccg ccgaacgcag cgtgggctgg gtacaggtgg taacagttgg tccatacgcg
     1321 gccagcctgg atgcctcggc ccatgcggaa ggcacgtgcg ccgtcacggc tccatacgcc
     1381 ggcgccaagg ccgtacatgg tgtcgttggc gatagccagc gcttcagcct catccttgaa
     1441 ggtggtcacg gacagcactg gcccgaagat ctcttcctgg aagatacgca tcttgttgtg
     1501 gcccttgaac acggtcggct cgatgtaata gccttcgctc aggtcgcctt ccagcttctt
     1561 ctgcacgcca ccggtcagga gttgcgcgcc ttcttccttg cccaggttga tataggacat
     1621 gatgatctcg acctgctcgc tcgacgcttg cgcaccgatc atggtgttgc tgtccagcgg
     1681 gttgccatgc ttgatcgcct tgacgcgctt gagcgcacgt tccatgaact tgtcatagat
     1741 ggattcctgg atcagggcgc gcgatggaca ggtacagact tcgccctggt tcagcgcgaa
     1801 cagcgtgaag ccttccaggc acttgtcgaa gtacgcgtca tcggcgtcca tgatgtcttc
     1861 gaagaagatg ttgggcgact tgccgcccag ttccagggtc gacgggatca ggttctgcga
     1921 agcgtattgc atgatcaggc ggccgaccga ggtggaaccg gtgaacgcga tcttggcgat
     1981 acgtgggctg gtggccaatg gcttaccggc ttcgacgcca tggccgttga cgatgttgag
     2041 cacgcctggc ggcagcaggt gttcgatcag ttccatgacc acaaggatgg aagccggggt
     2101 ctgttcagct ggtttcatca cggcgacgtt gccggcagcc agcgcaggtg ccagcttcca
     2161 ggccgccatc aggatcggga agttccatgg aatgatctgg ccgacgacgc ccagcggctc
     2221 atggaaatgg tacgccatgg tctcgtggtc gatctcactg atgccgcctt cctgcgcacg
     2281 gatggcgccg gcgaagtaac ggaagtggtc gatcgccagc gggatgtcgg cgttcatggt
     2341 ctcgcggatc ggcttgccgt tgtcgatggt ctcggcggtg gcgatcagtt caagattgga
     2401 ttccatgata tccgcgatct tcagcaggat attggagcgt gtggtggtgg aggtcttgcc
     2461 ccaggcttcg cgggcggcat gcgccgcatc cagcgccagg ttgacgtcct tctcgttgga
     2521 gcgtgcgaca cggcagaaag gcttgccggt cagtgggctg atgttatcga aatactgacc
     2581 gtcgaccggg gcgacccact tgccgccgat gtaattgtca tactttgcct tgaacggcat
     2641 tttggacgtt aggttcttca agctctcaag atacattatc catctcctcg tgattatagt
     2701 tttccaataa caccttgacc ttgcgatatg catgagcaaa ta
//
